LOCUS       DAJTRN010000001        94923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRN010000001 DAJTRN010000000
VERSION     DAJTRN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 94923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO328_000001"
     CDS             complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO328_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5928717.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1511..2164
                     /gene="rluE"
                     /locus_tag="OO328_000002"
     CDS             1511..2164
                     /gene="rluE"
                     /locus_tag="OO328_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5928718.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2174..2635
                     /gene="nudJ"
                     /locus_tag="OO328_000003"
     CDS             2174..2635
                     /gene="nudJ"
                     /locus_tag="OO328_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5928719.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2689..3795
                     /gene="mnmA"
                     /locus_tag="OO328_000004"
     CDS             2689..3795
                     /gene="mnmA"
                     /locus_tag="OO328_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5928720.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3831..4472
                     /gene="hflD"
                     /locus_tag="OO328_000005"
     CDS             3831..4472
                     /gene="hflD"
                     /locus_tag="OO328_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5928721.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4476..5846
                     /gene="purB"
                     /locus_tag="OO328_000006"
     CDS             4476..5846
                     /gene="purB"
                     /locus_tag="OO328_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5928722.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6015..6686
                     /gene="phoP"
                     /locus_tag="OO328_000007"
     CDS             6015..6686
                     /gene="phoP"
                     /locus_tag="OO328_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5928723.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6686..8146
                     /gene="phoQ"
                     /locus_tag="OO328_000008"
     CDS             6686..8146
                     /gene="phoQ"
                     /locus_tag="OO328_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5928724.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8943..9224)
                     /locus_tag="OO328_000009"
     CDS             complement(8943..9224)
                     /locus_tag="OO328_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928725.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9238..10899)
                     /locus_tag="OO328_000010"
     CDS             complement(9238..10899)
                     /locus_tag="OO328_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5928726.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10883..11209)
                     /locus_tag="OO328_000011"
     CDS             complement(10883..11209)
                     /locus_tag="OO328_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5928727.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11265..11447)
                     /locus_tag="OO328_000012"
     CDS             complement(11265..11447)
                     /locus_tag="OO328_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928728.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11431..11871)
                     /locus_tag="OO328_000013"
     CDS             complement(11431..11871)
                     /locus_tag="OO328_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5928729.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11871..12167)
                     /locus_tag="OO328_000014"
     CDS             complement(11871..12167)
                     /locus_tag="OO328_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5928730.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12164..12502)
                     /locus_tag="OO328_000015"
     CDS             complement(12164..12502)
                     /locus_tag="OO328_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5928731.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12499..13710)
                     /locus_tag="OO328_000016"
     CDS             complement(12499..13710)
                     /locus_tag="OO328_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5928732.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13712..14284)
                     /locus_tag="OO328_000017"
     CDS             complement(13712..14284)
                     /locus_tag="OO328_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5928733.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14324..15481)
                     /locus_tag="OO328_000018"
                     /pseudo
     CDS             complement(14324..15481)
                     /locus_tag="OO328_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15785..15946)
                     /locus_tag="OO328_000019"
     CDS             complement(15785..15946)
                     /locus_tag="OO328_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5928734.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(15956..16261)
                     /locus_tag="OO328_000020"
     CDS             complement(15956..16261)
                     /locus_tag="OO328_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928735.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16258..16536)
                     /locus_tag="OO328_000021"
     CDS             complement(16258..16536)
                     /locus_tag="OO328_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928736.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16825..18573)
                     /locus_tag="OO328_000022"
     CDS             complement(16825..18573)
                     /locus_tag="OO328_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5928737.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18570..18869)
                     /locus_tag="OO328_000023"
     CDS             complement(18570..18869)
                     /locus_tag="OO328_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5928738.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18875..19108)
                     /locus_tag="OO328_000024"
     CDS             complement(18875..19108)
                     /locus_tag="OO328_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928739.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19110..19330)
                     /locus_tag="OO328_000025"
                     /pseudo
     CDS             complement(19110..19330)
                     /locus_tag="OO328_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19323..19544)
                     /locus_tag="OO328_000026"
     CDS             complement(19323..19544)
                     /locus_tag="OO328_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928740.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19534..19734)
                     /locus_tag="OO328_000027"
     CDS             complement(19534..19734)
                     /locus_tag="OO328_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5928741.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19864..20043
                     /locus_tag="OO328_000028"
     CDS             19864..20043
                     /locus_tag="OO328_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928742.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20168..20497
                     /locus_tag="OO328_000029"
     CDS             20168..20497
                     /locus_tag="OO328_000029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928743.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20547..20735)
                     /locus_tag="OO328_000030"
     CDS             complement(20547..20735)
                     /locus_tag="OO328_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5928744.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21110..22339)
                     /locus_tag="OO328_000031"
     CDS             complement(21110..22339)
                     /locus_tag="OO328_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5928745.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22588..23709
                     /gene="roxA"
                     /locus_tag="OO328_000032"
                     /gene_synonym="ycfD"
     CDS             22588..23709
                     /gene="roxA"
                     /locus_tag="OO328_000032"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5928746.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23758..24984)
                     /gene="pepT"
                     /locus_tag="OO328_000033"
     CDS             complement(23758..24984)
                     /gene="pepT"
                     /locus_tag="OO328_000033"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5928747.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25234..26370
                     /gene="potA"
                     /locus_tag="OO328_000034"
     CDS             25234..26370
                     /gene="potA"
                     /locus_tag="OO328_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5928748.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26354..27211
                     /gene="potB"
                     /locus_tag="OO328_000035"
     CDS             26354..27211
                     /gene="potB"
                     /locus_tag="OO328_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5928749.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27208..28002
                     /gene="potC"
                     /locus_tag="OO328_000036"
     CDS             27208..28002
                     /gene="potC"
                     /locus_tag="OO328_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5928750.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27999..29045
                     /gene="potD"
                     /locus_tag="OO328_000037"
     CDS             27999..29045
                     /gene="potD"
                     /locus_tag="OO328_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5928751.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29201..30022)
                     /gene="cobB"
                     /locus_tag="OO328_000038"
     CDS             complement(29201..30022)
                     /gene="cobB"
                     /locus_tag="OO328_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5928752.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30038..30949)
                     /gene="nagK"
                     /locus_tag="OO328_000039"
     CDS             complement(30038..30949)
                     /gene="nagK"
                     /locus_tag="OO328_000039"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5928753.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30978..32222)
                     /gene="lolE"
                     /locus_tag="OO328_000040"
     CDS             complement(30978..32222)
                     /gene="lolE"
                     /locus_tag="OO328_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5928754.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKQIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32222..32923)
                     /gene="lolD"
                     /locus_tag="OO328_000041"
     CDS             complement(32222..32923)
                     /gene="lolD"
                     /locus_tag="OO328_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5928755.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32916..34115)
                     /gene="lolC"
                     /locus_tag="OO328_000042"
     CDS             complement(32916..34115)
                     /gene="lolC"
                     /locus_tag="OO328_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5928756.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34377..35450
                     /locus_tag="OO328_000043"
     CDS             34377..35450
                     /locus_tag="OO328_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5928757.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35578..39024
                     /gene="mfd"
                     /locus_tag="OO328_000044"
     CDS             35578..39024
                     /gene="mfd"
                     /locus_tag="OO328_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5928758.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39167..40129
                     /gene="ldtC"
                     /locus_tag="OO328_000045"
     CDS             39167..40129
                     /gene="ldtC"
                     /locus_tag="OO328_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5928759.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40212..40424)
                     /locus_tag="OO328_000046"
     CDS             complement(40212..40424)
                     /locus_tag="OO328_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5928760.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40709..41341
                     /gene="comR"
                     /locus_tag="OO328_000047"
     CDS             40709..41341
                     /gene="comR"
                     /locus_tag="OO328_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5928761.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41403..41942)
                     /locus_tag="OO328_000048"
     CDS             complement(41403..41942)
                     /locus_tag="OO328_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5928762.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42169..43473)
                     /gene="ndh"
                     /locus_tag="OO328_000049"
     CDS             complement(42169..43473)
                     /gene="ndh"
                     /locus_tag="OO328_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5928763.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43881..44423)
                     /locus_tag="OO328_000050"
     CDS             complement(43881..44423)
                     /locus_tag="OO328_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5928764.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44446..45471)
                     /gene="nagZ"
                     /locus_tag="OO328_000051"
     CDS             complement(44446..45471)
                     /gene="nagZ"
                     /locus_tag="OO328_000051"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5928765.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45482..46306)
                     /gene="thiK"
                     /locus_tag="OO328_000052"
     CDS             complement(45482..46306)
                     /gene="thiK"
                     /locus_tag="OO328_000052"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5928766.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46287..46928)
                     /gene="lpoB"
                     /locus_tag="OO328_000053"
     CDS             complement(46287..46928)
                     /gene="lpoB"
                     /locus_tag="OO328_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5928767.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46943..47320)
                     /locus_tag="OO328_000054"
     CDS             complement(46943..47320)
                     /locus_tag="OO328_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5928768.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47323..47682)
                     /gene="hinT"
                     /locus_tag="OO328_000055"
     CDS             complement(47323..47682)
                     /gene="hinT"
                     /locus_tag="OO328_000055"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5928769.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48015..50200
                     /gene="fhuE"
                     /locus_tag="OO328_000056"
                     /pseudo
     CDS             48015..50200
                     /gene="fhuE"
                     /locus_tag="OO328_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50260..51693)
                     /gene="ptsG"
                     /locus_tag="OO328_000057"
     CDS             complement(50260..51693)
                     /gene="ptsG"
                     /locus_tag="OO328_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5928770.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51988..52785)
                     /locus_tag="OO328_000058"
     CDS             complement(51988..52785)
                     /locus_tag="OO328_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5928771.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52796..53800)
                     /gene="holB"
                     /locus_tag="OO328_000059"
     CDS             complement(52796..53800)
                     /gene="holB"
                     /locus_tag="OO328_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5928772.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53797..54438)
                     /locus_tag="OO328_000060"
     CDS             complement(53797..54438)
                     /locus_tag="OO328_000060"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5928773.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54428..55450)
                     /gene="yceG"
                     /locus_tag="OO328_000061"
     CDS             complement(54428..55450)
                     /gene="yceG"
                     /locus_tag="OO328_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5928774.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55453..56262)
                     /gene="pabC"
                     /locus_tag="OO328_000062"
     CDS             complement(55453..56262)
                     /gene="pabC"
                     /locus_tag="OO328_000062"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5928775.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56382..57623)
                     /gene="fabF"
                     /locus_tag="OO328_000063"
     CDS             complement(56382..57623)
                     /gene="fabF"
                     /locus_tag="OO328_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5928776.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57710..57946)
                     /gene="acpP"
                     /locus_tag="OO328_000064"
     CDS             complement(57710..57946)
                     /gene="acpP"
                     /locus_tag="OO328_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5928777.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58157..58891)
                     /gene="fabG"
                     /locus_tag="OO328_000065"
     CDS             complement(58157..58891)
                     /gene="fabG"
                     /locus_tag="OO328_000065"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5928778.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58904..59833)
                     /gene="fabD"
                     /locus_tag="OO328_000066"
     CDS             complement(58904..59833)
                     /gene="fabD"
                     /locus_tag="OO328_000066"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5928779.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59849..60802)
                     /gene="fabH"
                     /locus_tag="OO328_000067"
     CDS             complement(59849..60802)
                     /gene="fabH"
                     /locus_tag="OO328_000067"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5928780.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60870..61940)
                     /gene="plsX"
                     /locus_tag="OO328_000068"
     CDS             complement(60870..61940)
                     /gene="plsX"
                     /locus_tag="OO328_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5928781.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62021..62194)
                     /gene="rpmF"
                     /locus_tag="OO328_000069"
     CDS             complement(62021..62194)
                     /gene="rpmF"
                     /locus_tag="OO328_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5928782.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62246..62767)
                     /gene="yceD"
                     /locus_tag="OO328_000070"
     CDS             complement(62246..62767)
                     /gene="yceD"
                     /locus_tag="OO328_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5928783.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62966..63550
                     /gene="yceF"
                     /locus_tag="OO328_000071"
     CDS             62966..63550
                     /gene="yceF"
                     /locus_tag="OO328_000071"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5928784.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63662..64621)
                     /gene="rluC"
                     /locus_tag="OO328_000072"
     CDS             complement(63662..64621)
                     /gene="rluC"
                     /locus_tag="OO328_000072"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5928785.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65194..68385
                     /gene="rne"
                     /locus_tag="OO328_000073"
     CDS             65194..68385
                     /gene="rne"
                     /locus_tag="OO328_000073"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5928786.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68582..69524)
                     /gene="flgL"
                     /locus_tag="OO328_000074"
                     /pseudo
     CDS             complement(68582..69524)
                     /gene="flgL"
                     /locus_tag="OO328_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69536..71178)
                     /gene="flgK"
                     /locus_tag="OO328_000075"
                     /pseudo
     CDS             complement(69536..71178)
                     /gene="flgK"
                     /locus_tag="OO328_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71244..72185)
                     /gene="flgJ"
                     /locus_tag="OO328_000076"
     CDS             complement(71244..72185)
                     /gene="flgJ"
                     /locus_tag="OO328_000076"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5928787.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72185..73282)
                     /gene="flgI"
                     /locus_tag="OO328_000077"
     CDS             complement(72185..73282)
                     /gene="flgI"
                     /locus_tag="OO328_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5928788.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73294..73992)
                     /gene="flgH"
                     /locus_tag="OO328_000078"
     CDS             complement(73294..73992)
                     /gene="flgH"
                     /locus_tag="OO328_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5928789.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74045..74827)
                     /gene="flgG"
                     /locus_tag="OO328_000079"
     CDS             complement(74045..74827)
                     /gene="flgG"
                     /locus_tag="OO328_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5928790.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74965..75716)
                     /gene="flgF"
                     /locus_tag="OO328_000080"
                     /pseudo
     CDS             complement(74965..75716)
                     /gene="flgF"
                     /locus_tag="OO328_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75736..76875)
                     /locus_tag="OO328_000081"
     CDS             complement(75736..76875)
                     /locus_tag="OO328_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5928791.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76900..77595)
                     /gene="flgD"
                     /locus_tag="OO328_000082"
     CDS             complement(76900..77595)
                     /gene="flgD"
                     /locus_tag="OO328_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5928792.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77607..78010)
                     /gene="flgC"
                     /locus_tag="OO328_000083"
                     /pseudo
     CDS             complement(77607..78010)
                     /gene="flgC"
                     /locus_tag="OO328_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78014..78430)
                     /gene="flgB"
                     /locus_tag="OO328_000084"
     CDS             complement(78014..78430)
                     /gene="flgB"
                     /locus_tag="OO328_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5928793.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78585..79244
                     /gene="flgA"
                     /locus_tag="OO328_000085"
     CDS             78585..79244
                     /gene="flgA"
                     /locus_tag="OO328_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5928794.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79320..79613
                     /gene="flgM"
                     /locus_tag="OO328_000086"
     CDS             79320..79613
                     /gene="flgM"
                     /locus_tag="OO328_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5928795.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79618..80034
                     /gene="flgN"
                     /locus_tag="OO328_000087"
     CDS             79618..80034
                     /gene="flgN"
                     /locus_tag="OO328_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5928796.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80074..81609)
                     /gene="murJ"
                     /locus_tag="OO328_000088"
     CDS             complement(80074..81609)
                     /gene="murJ"
                     /locus_tag="OO328_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5928797.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81809..82642)
                     /locus_tag="OO328_000089"
     CDS             complement(81809..82642)
                     /locus_tag="OO328_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5928798.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWCIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLCD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82644..83291)
                     /locus_tag="OO328_000090"
     CDS             complement(82644..83291)
                     /locus_tag="OO328_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5928799.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYLLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83302..83886)
                     /gene="rimJ"
                     /locus_tag="OO328_000091"
     CDS             complement(83302..83886)
                     /gene="rimJ"
                     /locus_tag="OO328_000091"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5928800.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84122..85330
                     /gene="mdtH"
                     /locus_tag="OO328_000092"
     CDS             84122..85330
                     /gene="mdtH"
                     /locus_tag="OO328_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5928801.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85394..86041
                     /gene="grxB"
                     /locus_tag="OO328_000093"
     CDS             85394..86041
                     /gene="grxB"
                     /locus_tag="OO328_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5928802.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86175..86735
                     /locus_tag="OO328_000094"
     CDS             86175..86735
                     /locus_tag="OO328_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5928803.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86841..87887
                     /gene="pyrC"
                     /locus_tag="OO328_000095"
     CDS             86841..87887
                     /gene="pyrC"
                     /locus_tag="OO328_000095"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5928804.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87961..88206
                     /gene="dinI"
                     /locus_tag="OO328_000096"
     CDS             87961..88206
                     /gene="dinI"
                     /locus_tag="OO328_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5928805.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88496..88750
                     /gene="bssS"
                     /locus_tag="OO328_000097"
     CDS             88496..88750
                     /gene="bssS"
                     /locus_tag="OO328_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5928806.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88865..89983
                     /gene="solA"
                     /locus_tag="OO328_000098"
     CDS             88865..89983
                     /gene="solA"
                     /locus_tag="OO328_000098"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5928807.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90031..90144
                     /locus_tag="OO328_000099"
     CDS             90031..90144
                     /locus_tag="OO328_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5928808.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90405..90971
                     /locus_tag="OO328_000100"
     CDS             90405..90971
                     /locus_tag="OO328_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5928809.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90975..91550
                     /locus_tag="OO328_000101"
     CDS             90975..91550
                     /locus_tag="OO328_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5928810.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91592..92644)
                     /locus_tag="OO328_000102"
     CDS             complement(91592..92644)
                     /locus_tag="OO328_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5928811.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92869..93789
                     /gene="lpxL"
                     /locus_tag="OO328_000103"
     CDS             92869..93789
                     /gene="lpxL"
                     /locus_tag="OO328_000103"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5928812.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93961..94158
                     /locus_tag="OO328_000104"
     CDS             93961..94158
                     /locus_tag="OO328_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5928813.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94182..>94923
                     /locus_tag="OO328_000105"
     CDS             94182..>94923
                     /locus_tag="OO328_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5928814.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRA"
BASE COUNT        22837 a        25174 c        23432 g        23480 t
ORIGIN      
        1 atatgctcga tatgctatta ataatatcca cataaataaa acaacgggcg tgttatacgc
       61 ccgtttaaat atttaacaca tgtagtgatt acatattctt gatgatcgca tcaccaaact
      121 ctgaacattt cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct
      181 tggcgttgat cgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca
      241 tatggcgtaa catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac
      301 cggcgtattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt
      361 ttgctccagg ggcgataccg ataccgccaa cctgcgctgc cagagcgtca gaaatgtagt
      421 caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt
      481 gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggta ttcgggtttt
      541 taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaattcttca cgcgccagct
      601 ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca
      661 gggtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt
      721 tggtgccttc ttcagaacac ggcttaatac cgataccgca atgttccggg aagcgaattt
      781 tcttcacgcc catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtcag
      841 ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg
      901 tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc
      961 agatgtagag atccagttcc tgacgcaggg caacgttgag agaacgaata ccgccaccaa
     1021 ccggagtggt cagcgggcct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt
     1081 cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt
     1141 ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt
     1201 tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg
     1261 gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa
     1321 caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac
     1381 cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat
     1441 gatttgtaat tgacttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta
     1501 ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag
     1561 aaatcaccag gttaagcgat tcagctcgca acgttctacc aggcgtaaac atgaaaacca
     1621 gcccacgcgt gtgatcctct tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     1681 aaccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     1741 ccttgaccgc gatagcgaag ggttgctggt gctgaccaat aacggcgcgt tgcaggcgcg
     1801 tttaacccag ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg aaggtattcc
     1861 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     1921 gcccgcaggc gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat
     1981 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     2041 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta
     2101 tgcgatggga gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga
     2161 ttaaggatgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg
     2221 caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     2281 gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac
     2341 cggcatcagc gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     2401 aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc
     2461 tcatgacagc gatatcgact gctgccgttg ggtcagcgcc gaagaaattt tacaggcgtc
     2521 aaatcttcgt tcgccgctgg tggcggaaag tattcgttgt tatcaaagcg ggcaacgtta
     2581 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     2641 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     2701 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     2761 ttgcaacaac agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac
     2821 ggtgaggaat attgcacagc agcagcggat ctggctgatg cccaggcggt ctgcgacaag
     2881 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     2941 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaag
     3001 gagatcaaat ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc
     3061 gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt
     3121 ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg
     3181 caaagcctgt tcccggtagg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     3241 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     3301 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     3361 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa
     3421 ggtctgggta tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa
     3481 gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct
     3541 gtcgggttga ttgcccagca gctgcactgg gtcgatcgcg aaccattcac cggcactatg
     3601 cgttgcacgg taaaaacccg ctatcgccag accgacattc cgtgcaccgt caaggcgctg
     3661 gacgacgatc gcattaaagt gattttcgat gagccggttg ccgccgtgac gccgggccag
     3721 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     3781 ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga
     3841 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     3901 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta
     3961 ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     4021 tcgggctgga aaccctgctc ggcgtgctca atgccagcag ttgccagggc ttaaacgccg
     4081 aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag
     4141 gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg
     4201 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc
     4261 cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
     4321 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     4381 tcggcggcgg acgtctacaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     4441 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     4501 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     4561 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     4621 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     4681 caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca
     4741 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     4801 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     4861 tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     4921 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     4981 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct
     5041 accgtatgga gcgccagcac cgccagctta accaggtgga gatcctcggc aaaatcaacg
     5101 gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt
     5161 tcagcgaaga gttcgtcacc tcgctgggta ttcaatggaa cccgtacact acccagattg
     5221 aaccgcacga ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga
     5281 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     5341 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa
     5401 actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc
     5461 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     5521 gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag
     5581 tgaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gcagaaccaa
     5641 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctta
     5701 ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc
     5761 cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta
     5821 tcacgatggt tgatgagctg aaataagcct caaatcagtg ccggatggcg atgctgtccg
     5881 gcctgcttat taagattatc cactttttct tttttcactt tacctcccct ccccgctggt
     5941 ttatttaatg tttaccccca taaacacata atcgcgttac actattttaa taattaagac
     6001 agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc
     6061 ttaaagttca gattcaggat gctggtcatc aggtcgatga cgcagaagat gccaaagaag
     6121 ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc ggattgcctg
     6181 acgaggacgg tctgtcactg attcgccgct ggcgtagcaa cgatgtttca ctgccgattc
     6241 tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt gccggtgctg
     6301 atgattacgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg caggcattaa
     6361 tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct ccccccgttt caggttgatc
     6421 tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca
     6481 ccattatgga aacgttgata cgcaataatg gcaaagtggt cagcaaagat tcgttaatgc
     6541 tacaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac
     6601 gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt cattaccacc gttcgcggcc
     6661 agggctacct gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc
     6721 gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt cgcttgccta
     6781 cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt
     6841 acgtggcgag agcaatctgt tctataccct tgcgcagtgg gaaaacaata agttgcatgt
     6901 cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa
     6961 cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaaaatga tccagcctga
     7021 ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt
     7081 gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgatgacga
     7141 cgcggagatg acccactcgg tggcggtaaa cgtctacccg gcaacatcgc ggatgccaaa
     7201 attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg
     7261 gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt
     7321 cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga
     7381 agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg
     7441 aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct
     7501 caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc
     7561 tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg agcaaatcag
     7621 ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt
     7681 gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct cagcgctgaa
     7741 caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt
     7801 tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata atgcctgtaa
     7861 atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt
     7921 ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg
     7981 tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat
     8041 caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat
     8101 ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccttttac
     8161 cactacatca agtcattgat tcgatcaaac aattttcatt gaaaaagtgc caaaaatcgc
     8221 aaattgacta caccattaac tacaccgttc ggtgcactgt atgaaacaac gtggaacaaa
     8281 tagacacaag aaatatacag gcgggtaatc tttccagggg gaagcgcacc aattcatgag
     8341 gggcgttaat gtcgatatgg ggatccccat aacggggcta ccggattttt tttccggtta
     8401 acctttaatc aggctggtgg gttttacata cccttgatca ccgtaatgat gatccagatg
     8461 tgcacctttt ccaccaatgg cggaaaagct ccaaccgtaa cgatgatcgc tatggttgcc
     8521 tcgatggtct gcgtggtcat tatgaccgtt cagccagtga ccttaatcag aatttttcgc
     8581 taaaaaatga cgttggccac gtcatccgga aagcacaaaa tcacgtcgat ttttaatgaa
     8641 cggttaaacc gtctttttgt cacggtgtgt cactggttgt cactctccgt cacgattgag
     8701 gtggtggtca ttctgcccga ccgcgcatct ttgcgctatc gggaatatca aacagttacc
     8761 tcgccgactt cctcaaattg aggtttccga aaatatcaga gcgggttagc gattaagctg
     8821 acgagtaatt aaactgcgaa aatttcgtag tttctgaata tattccggcc tgctggttaa
     8881 cagtcctcag atgtgaggga tgtagccagc gcaattttgc gctttgcttt aatctcagtg
     8941 agttaccgcc gtaaccgctc cggcttcttc cagtggtacg tgattttctc cttctcccga
     9001 tacagttcaa cgcggcgatt gtaggccagc atttccagaa cgcggatccg tatgtcgcgc
     9061 atatccgcat cattaagctg gataccatca cggcgcatca cctcagcaac aacacgcgca
     9121 taattttcgg ctgtcacgct gtccggctgc gtggtctgtt cgtcagcctg ctggctgatt
     9181 ccagagacgc ggcggattaa tcgcagtatt tcggcttctg tcatgctgct gacctcatta
     9241 caccccgcta aattcttcca gtttctggcg gtggctgtcg cttatatcaa aagcaaaatc
     9301 ctcatgctct gcctggaatg taccaaacgc catcagcgcc gctacgctcg ggtctatctt
     9361 gttcggtgat tttttcttgt tcggcttgat attggcgttc gcgtcactct gcatcacgac
     9421 gttactcatc gaccaggaca acaccggatc gccacgatgc acaatcaccc tgcggttaac
     9481 aaaaacttcg aacgatttcg ccgccggact gaatctgagg taggtttgcg ggaacggctc
     9541 cacctcaaaa ccagccccct gtaattgcgt cctgagatgc gtggcgttcc acgtatcaaa
     9601 gcccaccagc ctgatattaa atttctccgc atcctgcatg atgtcatctc tgatccggtc
     9661 gtaatcaatg cagtcacctg gcgttgtgcg tatccagccc gctttagccc actggcgata
     9721 gacagcgcgg tttttattgg cggggttctg tagctggaac tccggcagat aatgacggga
     9781 aatcagcata atggttttac cgaccggaaa ggcatagcat acgctggaaa tatcgctggt
     9841 tgatgataag tccagccccg cgtaacactc ctgaccgtgt aaatcttcct ccgtgaacgt
     9901 tccggcacac tcagcccatg caccattacc catccacggg gtagtcccct gacaccagat
     9961 attaaatcgc tttgtcatca tctccaccca ctgcgacgga ataccccgcg ctttctggat
    10021 ggttgaggcc agtttttcac gatccacgga aacatcaata ttagggttcg cctttatcca
    10081 catcgccgga tcatcaacct cgttttcgtc gtccagttcg taaatcagta caaaaattga
    10141 atcgttaacc tcttcgccgt ccaggatctg gcagcaataa tcatagtgct gtttacaggc
    10201 tgaaacgacg ttactgcccg atgtggtaat ggcaaataac agcccttcgg gacgtgcgcc
    10261 catccccagc tcaagcgcgg aataaacgcc gttatcgggg tgtaggtggt attcgtccac
    10321 gatggaaaga ctggggtttg tcccctcgat ggttgccgct tttgctgcca gcggctttaa
    10381 caggctgttg cttttcgggt gtatcacctt atgcgcctga atattcaccc gcctgcgtaa
    10441 cggtcgggat aaaaggcaca tctgacgcgc attatcaaac acgatccgcg cctggtcacg
    10501 gctcaccgct gcggtgtaga tatcctgctg accgttctcc ataatcagaa accagttagc
    10561 cagaatcgcg gcggtcgtgg atttcgcatt tttgcgcggc acttcgataa aggcgctcgt
    10621 gtatttgcgc cgtccggtgg ccttaacctt aaagccgagg atgcacgcaa aggcgaactg
    10681 ctgccacggc tccagctcaa tgggtctgcc acgcatcggt ccttttacgt gcgggcacac
    10741 cctggaaaag gcaataaacc gctccacaac ctcacgatcg aacgtgtaaa gggggctttt
    10801 aaggtccgaa aagtaccgtt taacagcctg ttttaaccgt ttacaggccg gaattttgcc
    10861 cgtttttacg tcttctgcgt acttattcca ggcggtcaag ctcgtcctct tcttctgttt
    10921 ccggtggatt tttacggcgg cttatcgggt caaaaccgag caaggaggcc atttttatca
    10981 tcactctttc agcgtcggat tttgcgctta atgcggggtt tctgctctcg ccgccctgac
    11041 tgttaacaat gctgaacccg cgcgccgcaa ggtctgcaac ggctttccgg taaatggagt
    11101 agttgacgca atacagttcc agattgctcc agtcggcggg ggtcaggtct ccccgttccg
    11161 caagctgccg cgatttttcc cgccactgct tcacggcgat atcatccagg taggcggggt
    11221 cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc ctcatcagcg attgcggaaa
    11281 cattccataa cctcgcggtc acggtcggtt aatcgcttcg ctgtgatgcg ttctgcgcgt
    11341 cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc atgcgtcacg ctgccttata
    11401 agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgtcataca tccagttatt
    11461 gcggttagca gcccgttctt cctcttcacg aaatccacct gcggcacgct tgctttttgt
    11521 tgccggatca agccatttcg ttttctggtt atgacacgcc tggcacaatg gttgatggtt
    11581 ccattcgggc cagaagagaa catcatcacc gccattaatc gggataatgt gatccaccac
    11641 cacggcgggc gtatatatcc ccttctcaag gcatcgcacg cataacgggt ttttactcag
    11701 atacatggcg cggtatttgt cccactgtct ggaatatcca cgcgcgcggc ggtgtcctcg
    11761 tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc tcacacttgc cggatttaac
    11821 gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt tgccacggca tcagtacccc
    11881 cccacatcac ggtaagccgt ccagagtgcg cctatcgtca tgggtacgcg tgtttttgcg
    11941 ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat gcagccaatc
    12001 tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc tgcctatgtg tatctggcac
    12061 gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc gccatcgatg
    12121 cggcaatgga gtttcacttc ttccagggtg ataagttctt ctgtcatttt tccgcgccct
    12181 cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg cagcggctgc ccgatgatat
    12241 tcagtacacg ccccgccagc ggccccgtac tgacctttat ccggctggtg gcgttgatgt
    12301 ccttccggta ccgtatccag atccttacgg taccaatcgc cagctctgcg cccgatgaaa
    12361 tggcctcctt gctgctgacc atgttcacgc ttgcccagag tgtgtgaccg tcctcccacg
    12421 tttcgagtat ttcgccggtt atatccctgg tctgtttcag ggtctgaatc gttatcctgt
    12481 cacgcagtcg ccctatgttc attccggttt ttctccctcg ctgattttta cttcctgttt
    12541 ccatgcctgg ctgaactcat caccaccatc acgcggtgac agcccttcac gttcgcgggc
    12601 ttcgttcgga cacataacgt cggattttat gccgcgctca taggtggcaa agcgttcgcc
    12661 aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc tgataccgga taccaggtac
    12721 aggcgaggcc accagcaagg cgttttttat ctgctgctca aagttcgcca gccacgggcg
    12781 catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag ttgctgtagg tgctgttgct
    12841 gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg gcgatgtctt caatggtgaa
    12901 gcgacgggag gccagccatt cggcatcctg attactcatg cccagctgct gatagctcat
    12961 ccccccttca aggatgggcg tttttccggc gtttctggcc cctttgtagc gttccagtgc
    13021 ggctaatgcc tgtttgccct tcacgccgtc cagccattcg cctgacgtga taacccctgc
    13081 cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt tgttgggcca gcccaagccc
    13141 cagcgattcg cggcagatgg ttacaggtga gcgccccata aaaccgtcat ccgtggagta
    13201 acgaaggtgg agaacctccc acggtaaata gttgcggatg tttccggtgt aggcgtcagt
    13261 gatgcagtag cgccagttat gatcacctgt ctgctccacg ttcaccgact gcggcgggta
    13321 aggatgtaaa gccgccggaa aaccatcacg cccccactga ataaccgcat aagcattacc
    13381 acttaacagg cagtagcgga tcatcattcg cttaaactgg tagggtgtct gccacgcgtt
    13441 cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca agccattcgc gggcctcctt
    13501 ccccttctca ttgcgtacca ggtacaggta acacggcatg gtagccaccg cgtcagagat
    13561 gaccgtgacg gcgttcatga cggcggggag tgattccgct gtcccagatg atacgtactc
    13621 acccgccccc gtgttcgatg tgccggccag cgccataaat tcatcaagcg tcatgctgcg
    13681 ctgctctttt tttcgtctga aaggccacat atcacacccc cgctaaatcc gcccaccagc
    13741 ggcgattatc cgcacgcggc attttttcgg ggtgctgctc atacagggaa cggcgggcca
    13801 gctccacgcc ggaatcgggg taagccggta ccgatgtaac ggtaatttcg tacagttccg
    13861 ccaccagcac ggtgcgcacg catggatctg ttgtggtatc ccatacatcc ttacgggagc
    13921 gaaagccaaa gctcatcccc gagatgtccc cacgcttaac cagttcgata acgtcgcgcc
    13981 ctgtactggt atccggtggg gtcagttcaa agcgtaaccc tgtttcgtcc tcttccagtt
    14041 tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg gtcatgctca tacaggccgc
    14101 gaacgtcatt acccgccgca agccattcag taaacgcccc ccgctggaat ttttcgtaaa
    14161 actcacccca taacagttct gaaagcttat cccagcgaac aacgtagccc gtcagcgtac
    14221 cggctccgct ggtggtgatt tccgatgacc ggatttccat actcttcata attttttcac
    14281 ccataaaaca ctaaaggggc tttttagccc cttctgtatg ctgttaatcg tcgtcttccg
    14341 gcagtgccag aatcttgatc gcgttcgaat ccaccacgcc accgcctaaa tatttttgcg
    14401 tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg tctggtgcga acacctgttt
    14461 cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag ggcaataacg ttatcaggca
    14521 taaactccag atattcgacc ggaaggccca gcaacgtatc aggatcaccc gcctgtaaac
    14581 ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt ctgaagtttt gccgccgtcg
    14641 tggagttaac cactcagacc gcgtttttgc ggtatttttt acgtaatgca aatttcaggt
    14701 cgatcagcgg gtccgcagat gtccacgcca gagattcgga aggtttaatt acctgcaacg
    14761 taccaaaatc acgctctttg tcgttcttct ctgcacgggg tacggataaa aaaccttttg
    14821 cttttttgtc accgtcgccc acaaccagat cgctttcttc ggtttccgtg aaggtgtcgc
    14881 caatctcacc cgtcagccat gaaaggatgt ccacatcgga aaaatccacg atttcctgtg
    14941 tggtgcgcgg gtacgcatag accggataca gcttaatgct cacctcgtta atctgcgggg
    15001 tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt aacggtcgca ccgccagcgg
    15061 aaacaagctg cttaaactcg ttgctgctga ttttcttcac ggtacagatg cgacgcatgg
    15121 tggattcatc cgccagcatt cgcatgattt cggtgttcag ttcagggata acggtataac
    15181 caccatcagc gggaacgcct gtacttaatg cgcgggtttc accggtcaga atgtagttac
    15241 gtagttctgc ggggtcagtg gtctggctgc ttttacctgg cttgctgcgc tcttcgtctg
    15301 caatggcttc aaggcgggaa atgtctttat cgagggattc agctttagcg cgtaattcgt
    15361 caaattttgc gccctcagca tcgttaagac tgcggttttc tttttcagcg ttctccagca
    15421 tgtcgcgcat ctgattttta atggcggttt tctgctggcg taattcgatt attctctgca
    15481 taaaaaaagt cctggggtta agtaaggaac tccaggacgc ggcaaacact caaccgtttt
    15541 tcataaggaa atcagtaatc gcaccgatcg ttttcccgcc tggtaatgaa tattggcgag
    15601 cacattaaca ggcgggaaag tggccccagc gtcctggcac cacgggcgag aataatcatg
    15661 attcagttcg ggtaaaatat gccgatccgc tcagtgaaca acgtggaaca accacgaaca
    15721 aataatttac aaaaaataac aaaaagccgg attgctccgg ctgcgtggtg tggttctgat
    15781 attcctactc tgctaatttg ctcagcccca tgtctgcgta agtgcgattt actgcatttc
    15841 tcaggtctgc gtagttctcc gttggcggct ccggtgtgcc cttgctgctc tgttttacca
    15901 gcgggtcgcc caccagctgc ccgtaagctg caatttgtgc tgtcatattg ccacctcagt
    15961 gaagcgatcc ggcattgttc tttttcatct cttccatcat tttttcagtg agcattacgc
    16021 actgaaaccc catgtttttg atgtctacgg gtttcatgcc tttgtcagtg gatatcacag
    16081 gcagaatatc ctgatacaca tcgcggtcag ggtaactaag cacacaaagc tcccatgatg
    16141 ccacgggtga ggatccttcg tctgtttcaa cccaccagcc tggtgtagct gcgcttattt
    16201 gcagaatcgt gatccgtccg ttgtgcttgt aaaatttttc accaaatttt ggttcgctca
    16261 tgattccacc tctccggttt taacgttgat tgttgttacc tgttccgctt cggcaatctc
    16321 ccgctctgtc agcgtggcaa aatttgccgc cgtcgtggtc atgaatgcgc ttatcagttc
    16381 gggatgtgct ttcgcgtatc cttcccccgc gtggcggtct atcgttctga ttgccacctt
    16441 taaagcgtgc tctgtcatgt ctaacgcgcg atatttcggt tctgttctgt ctctgcgttt
    16501 tttgaatgat ttattaaatt tccctgaagt gtgcatattt atttttaccc cctcgtttaa
    16561 aaagttttga gttgtgcctc cccttgtcta ccttatctac cttagtggcc ctcatgccag
    16621 taatggcgct gctttcagcg gggtagagtg cttttatcca ctatctaccc cgtgtctacc
    16681 tccctgtctg attcaggtaa aatcaggtag agagggtaga tagtgggtag acagtaaaaa
    16741 aaggctatct acctaactta atgcactgaa ttaaatgtat ttttctttac tcaggtagac
    16801 agggtagaca gcaattacaa aaaattataa aaacgcgtcg cactcgtctg ttgttattgc
    16861 gttagtctgc gttactccct taactttccg cgtaatatat tcatgtccgt aaactttcgc
    16921 ggctggcttc atagccttgc caaagtcatt tacgtttagc ggtttgctcc tgcctgcgta
    16981 cgccataaac gccagataga cgcggtaaag gctgtttctg gtcgtgtact tcactgaatc
    17041 gccaccgccg cccatcatca ggccgcgtgc ttcctccaga aaattcagga actggcaaaa
    17101 ctcaataacc ggatccgtct gttgctttat tgccagagct tcatcaccgt cacgctgttc
    17161 cagtagtaaa gcccgtgctt tctcagagtc ggtaaagttc gccagcaatc ggcggataat
    17221 gacagggatt tcagccgcaa tcttttccgg tagctccctg tctttttcgg cctcactaac
    17281 gatattgtcg aaacggaaaa tcacgcgacg acgtgccaca cctccggccc gttcggtgaa
    17341 tatcatcggg ttattgttgg tcgccagcac caccgccctg attacagccg tgaaacgctt
    17401 ttcgtatttc gggttaattt ccacggggtc accgcccgtg attttcttga tgcccgttcc
    17461 ttcgcctgta tatttcggct ggtcagccag gacgataaga cgactcccga caacctgcgc
    17521 acgtccacca gcatcatcaa gcgatgtcat ttcagcgctt accgtgttct gtttccctgc
    17581 cagaaggctg gctatgtgtg tgaatgtact tttaccgctc ccgccgtctc cggtggcctc
    17641 aataaacatc tgccagtcgt accggttcgc cataatcatg tacagcgcgg cacatatacg
    17701 catcatcttg cgctggtctt ttccggctgc gtgctcaagc catttatgaa agtttagcgc
    17761 gttatcgcgg atgttctccc ctggtgctgg tggcgtgtac tcaatgccgt tgtgcgtggt
    17821 gatccagttc tccggcgtgt gcggggaaaa ttcccccgtt ttcaggtcaa gcgcaccatt
    17881 ggcgaacggc agcaaatcgc cggacggctc gcccattggt tcggcaataa cttttaacgc
    17941 ttccacggcg ttattgatta cgcgcttgct gaaagtggcc ctgtgctctg aatagatcac
    18001 caccatttcg cggctaagtt ccattgtgct gaccggacac cataccccgc cgcgccatac
    18061 gtgaacgatt tcactttcct gatgcacaca aacgccatca aagcgctcgg caagcagctg
    18121 cgcgcgctca ctgtctgcca tttgtgccag ttgcgtattt ttctgctccg gcatggtaag
    18181 ccctgcggca atattttcac gctcagcatt cagatagcgc cgccagtttt cacacttctg
    18241 gacgtgcatt ccttcggggt aaaaatttgc gtcctgaata tctgccgccg ccagcttctg
    18301 accaatcttt ttggtctcca ccaaatccag ttctccggcc tggtacagcc ttacgcgttt
    18361 ttttccatcc ggaacaattt tcagtgcatc aagttcggca agctgatttg gcccaagcca
    18421 cacaggcggc acattatcgc cggatgcggg gccatcctgc tcctgccatt gtttcgcgtg
    18481 cgaccaggca tcactacccg caaagataat tacctctgtg tctttgtgtt ttattccgcg
    18541 tggctgtttt tttacgttcg gtgccagttt cattttttac ccctgaatac gttaagcatc
    18601 ttttttattt cctgaatatt ggcgcgtgct ttctccctgc tggttggttt actgcggggc
    18661 gctgcctgta ccagcgtgaa atcacgccgg aattgataaa caggcatcac gcaatcatat
    18721 tcgtaacctt cacggcggta ggtgatgcgc cgttctgcca cgcctttaat cgttaccatg
    18781 ccgccgtact ggtcgcggta aatatcaccg cgcgtaaatt tagggtgagt gttgccactg
    18841 gcagttaagc cagaatattt aagtttcatt atttttattc tccggtgtgt tgttctttat
    18901 atctgtcgtg caatagatct atttcttgca gttccattat tacaggctca agaagcgtta
    18961 ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc attgtcgcca agtgtttcaa
    19021 gccatatgcg caatatttcc agcatgtttt cactgtgaga aagtgcaaga aatgcgcggt
    19081 ctattgtttc gtggtaaata tcacgcatat taatccccgt ccgtcgtttt tcttaaaata
    19141 acctctgtca cgaaatcagc ataatcggcg gcgatattca gtacatcaag cccgttgatt
    19201 tatattctct cgtggaaagt aagaaaaaag ccgctctaat aagttctggc attgacgaaa
    19261 gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg tttcagggaa ttaaaacgat
    19321 catcacgcat aacccccccc attttcacaa tcggcaacaa gaatattttt agcgtcattc
    19381 agtgaccgcg ttgcgatgta tcggatacat tccagggcga actgtgtccg gtctttctgt
    19441 tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa taagcgcgtg catcagcgta
    19501 gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt gcataggttt ccccctgacg
    19561 aatacgggcg gcgaatacag caacacaacc ggacgggcaa cggctacgcg cttcgcgttc
    19621 cgtccaagcg gttacgtgga tgatttggga ttctccggca ctcagtgcca gaaaacgcca
    19681 cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga tttacggcaa ccataacggc
    19741 tcctagttta cgttgttggt tagaagcccc gttactgctc ctacagtgcg gggctttgct
    19801 ttttgtgttg attgtgtgta atgtgtcact acacataaat acattacctg aggtgtaatt
    19861 aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc cgttccagtt ccgtttagat
    19921 cctgacttac gggaaaagat ggaaaaggca caacaacagg acggcgatga ttctcttgct
    19981 gcctggataa agcgaataat ccgcaaagaa ttacagcaac gcggcataga acccaaagga
    20041 taaaaactat cagcgccgtg gtgtgaggta ctacggcgca ttgctttaca ggcgcacaca
    20101 atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac gaaaaagacg acgcagaaag
    20161 aaagagcatg aatcagaacg attcgcgccg tgttcttttg cccttgagaa gtaccttaaa
    20221 gaatactcat caaaggagag aacttcgcag ttatggcaac gcactgaacc agactgattg
    20281 cattgcccac cagcctgata gcggctatca tccccgtgct tatgtttggg atcacacaca
    20341 caaacggcgc agcgggttat ctgttcagaa aggcggctcc atttcggggc cgcttttttt
    20401 atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac cagggcgaac gaatccccgc
    20461 ccacgttctg gcgtatattc aatcttcatg gttatagctc tgtgttcaga tgattgatgt
    20521 gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct tgcagctatc cttccaggcc
    20581 agaacctcgg ataaagacca gccaacggaa cgaccgccaa gtttacgacg tgatgggaat
    20641 tgtccggcct tttccaggcg gtagcggcat gagcggctaa ggcctgttag cttttcgcat
    20701 tctttttcac gtataaaccg atcagtgctt aacactattg ccccctttcg tttcttaaag
    20761 agttatttcg tgttctattg cgttgggatg tgtctgattg tgtcaggatg attcagagtt
    20821 ggcaaatgtt gaggccgtat ggtttacaga aagaggaata atcaggataa aatcctttaa
    20881 attcatgtta atacaaaggc ataaaatatt gtttcatgcc ttttttctcg ctatttaaag
    20941 agtgattcgc tagtgtataa aaaatcagta acacattaaa aatcagctac ttataaatct
    21001 gtacactttt tcacctcttg ttcgtagttg ttccgcattg ttgctcattg ttgcacgttg
    21061 tatctgttcg catatccagt atgcgcatac tgaaaaaaca cgaaaaaaat tattttcttc
    21121 tggctactgg tagcgtggtt acgttttcat gtgttcccgc cagtatccct aaccgctccg
    21181 tccacatatc cagcgcattg cgtttagcat ccagataacg ggaatgatta taaactcgct
    21241 gcattcctgg catctggtgg cctgtaagct gctccacgac gtgaggatcc acgcctaaat
    21301 cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag tgaccagtga gggtgattta
    21361 gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt ttcctgtttc acttccccca
    21421 gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt gacgaacggc agtattgctt
    21481 ccggtatggg ccggaatatt gccaccttcg ttttgctgtg ttctttcggc acggtccaga
    21541 gcatttcggt aaaatcccac tcgctgatct ccgataacct cagttctacc gtccggcatc
    21601 cgaacacaat caggaggcgg attaacgcga tgtagtaggg ggagaatatt tttttgtcca
    21661 gtgcctgcaa taattcgccc agttctttgg tgcttaagac acgctcgctt atatccggtt
    21721 ttttcccaac gtccgccaca ctcatatcat caagaacgtt gctgattgca tagcgccgcc
    21781 tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa gaatccggca gtaacaggcg
    21841 ttcgctttgc cacctggtca aaacaggcca gccagtgccg tagctcgcat ttatccagcg
    21901 gcatagcacc aatgtgctgt attacgtgat tattaaggcg ctttttcagg gcggcataat
    21961 ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt gagcgcatcg ccaaccgtta
    22021 ccggctttaa cgcttcctgt acggtgtaat taagctcatg acgtggattt ttcccctctg
    22081 ccagccatgc gcgacactgg gcggcttttt ccctggctga tttcaggctc agatcaggat
    22141 aatttcccag cttaatgcgt tccggtggtg ccccccttcc cgttccggcc ctgtaagtga
    22201 aataccaggt taaaaggcca ctggttgaat gcctgacgct caggtttcca ccgtcattaa
    22261 gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct cagctgtgta tcgctcagtt
    22321 tgttaagtgc tctgctcata attttgattc cggtcatcac aattaactac accattaact
    22381 acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc tggaacaagg aaaatcacaa
    22441 atctgtttat aatcaaaaac ataatgaaca atctgaaaca ttatgaaacg gcaaaaaaca
    22501 ctaattgata aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt
    22561 gcagatacca gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat
    22621 tttcttgaac gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt
    22681 attgacccga tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt
    22741 cgactggtca gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac
    22801 gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg cagtaaacca ctggcatgag
    22861 ccgaccgccg cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg
    22921 atgatttctt tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg
    22981 tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa
    23041 cagcactgcc cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa
    23101 gagctggagc ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg
    23161 ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt
    23221 agcggatttg ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatccg
    23281 gatgttccac ctcgtgctca tcctgcggat gttctgccgc aagagatgga taaactgcgt
    23341 gagatgatgc tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt
    23401 atatcccagt cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat
    23461 gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta
    23521 ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg
    23581 gcactggatg cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg
    23641 gaagatccgt cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc
    23701 gaagggtaag tttggattta agccggatgc agcatcacat gccgcatccc ttttcgctta
    23761 cttccgttgc gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc
    23821 ttccagaatc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
    23881 gcaaggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
    23941 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
    24001 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccaatt caatgtagca
    24061 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
    24121 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt
    24181 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
    24241 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
    24301 tttcgccgtg cccggatgaa cattgttacc gacaattttt atattgaccg atgcggcgtt
    24361 gaagttttca aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc
    24421 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
    24481 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
    24541 gatttctgca atacctgctt tgtcatcagc acctaacaag gttttaccat cggtggtaat
    24601 caacgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
    24661 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt
    24721 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
    24781 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
    24841 gataagcccc atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
    24901 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
    24961 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
    25021 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
    25081 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
    25141 caagcggttt gccgtagaat taccgccctc attaggagtc accaaggtgg ttaaccacaa
    25201 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
    25261 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
    25321 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
    25381 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
    25441 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
    25501 tatgtgaaca ctgttttcca aagctatgca cttttccccc acatgaccgt gttcgaaaat
    25561 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
    25621 gaagccctgc ggatggtgca gctggaaacc ttcgctcaac gtaaaccgca tcagctctct
    25681 ggtggtcaac agcaacgcgt cgccattgct cgagcggtgg ttaacaagcc tcgtctgttg
    25741 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
    25801 ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt tcgtgactca cgaccaggaa
    25861 gaagcactca ccatgtcgga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
    25921 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg gttcattggc
    25981 gaaattaata tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc
    26041 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
    26101 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
    26161 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
    26221 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgat
    26281 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
    26341 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
    26401 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
    26461 gacccgcgac gacgccagtt tcgtcaaaat ggtctttaca ctggataact acacgcgtct
    26521 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
    26581 cctcgcctgc ctggtgctgg gctatccatt tgcctggttt ctggcgaagc tgccacacaa
    26641 ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc tggaccaact cattgattcg
    26701 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
    26761 gctgggcgtt atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg
    26821 tctggtttac attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact
    26881 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
    26941 ccgcatcatt attccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct
    27001 gccagcgatg ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat
    27061 cggtaacgtc atcaaggtac agttcctcaa tattcgtgac tggccgtttg gtgcagccac
    27121 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
    27181 tttgctgaat aagaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
    27241 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
    27301 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
    27361 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
    27421 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc
    27481 ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga tgtcaccaga tatcgtgatg
    27541 gcaatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
    27601 ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg
    27661 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag cgaatttacc
    27721 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcggg ctgggtgtta
    27781 agctttaccc tgtcgatgga cgatgtggta gtttcttcat tcgtcaccgg accaagttat
    27841 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
    27901 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
    27961 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
    28021 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
    28081 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
    28141 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
    28201 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
    28261 ataaaatgcg taaagagggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
    28321 gcaatctcga tccagacatg ctcaacaagc cttttgaccc gaataacgac tactccattc
    28381 cgtatatctg gggtgcgacg gcgattggtg ttaacggtga tgcggtggat ccgaaatctg
    28441 tcaccagctg ggccgatctg tggaagccag agtacaaagg cagcctgctg ttgaccgacg
    28501 atgcccgtga agtgttccag atggcgctgc gtaagctggg ctactccggt aacaccaccg
    28561 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
    28621 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
    28681 tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac gtggtgtggc
    28741 cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
    28801 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
    28861 ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agtccagaag
    28921 tggcaaacga taagacgctc tacccggatg ctgaaaccat taaaaatggc gaatggcaga
    28981 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
    29041 gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
    29101 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccgcttgt
    29161 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc tccagcaact
    29221 tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
    29281 taccaacctg actcggttca agattcagtt caacggtgtg agcaccatgt agtttggctt
    29341 cgtgtacaaa cccagccgcc ggatagacat gcccggaagt accaatggca ataaagacat
    29401 ccgccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaaa
    29461 cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct tctggcgtaa
    29521 cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc agcaattccc
    29581 catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
    29641 ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt agccagcgca agatgcgcgg
    29701 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
    29761 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
    29821 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
    29881 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
    29941 aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt tttacgaaaa cgacttaacc
    30001 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
    30061 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgt ggaacacgag
    30121 ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg cgttgtgatt gccgggaaat
    30181 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
    30241 gagaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
    30301 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgataca
    30361 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
    30421 agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc agacgcatat
    30481 ggccaaactc gccggtaatg tagcttttac cagtaatcgg tttgccgttg aaaaccagcc
    30541 cgccgccaac gccggtgccg agaattaacc ccatcaccag tggatattgc gtaaattcat
    30601 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
    30661 caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct ggcagcgggg acattagcgg
    30721 catacagctt accatcttct gtttccggca ttcccggaat accgatgccg acagaacctt
    30781 tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct aaaaatgcgt
    30841 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
    30901 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
    30961 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
    31021 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
    31081 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaataaat atcgctggag
    31141 aggaactgat gaccgatctg cttttcaatc cactcaataa tcggggtaag ttgcagtgaa
    31201 acaactacgc cgataaccac cccacacagg ctgccgaaca gccctgccag caatccatac
    31261 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
    31321 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
    31381 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
    31441 atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt cacttcaccc
    31501 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
    31561 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
    31621 tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc
    31681 tgcatcagtt tatgctcggg attcgagttg gggatcatga tcgacaccca atcgccctgc
    31741 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
    31801 aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
    31861 tgtgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc caccagaccg
    31921 gtgaaattga tatacggcgc agcggcggca atacccggca ctttctgcac gttatccagt
    31981 gcttcctgcc agttagtcca tggctgattt accgcttcga tttcgccatg cggcaccacc
    32041 gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
    32101 aataccgcca cgccgagggc aatgccaatg gtagaaatga cagagattag cgacaccatg
    32161 ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca ataacgataa aggcatcgcc
    32221 attactccgc ccccatcagg ctcagttccg ccgtcagacg tccatcacgc atttccagtt
    32281 ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac caccaggaaa gcggtgccct
    32341 gcaagcgatt caactcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
    32401 taccggtagg ttcatccgcc agtaccagac gcggattatt gaccagcgca cgggcaatcg
    32461 ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
    32521 gccccaccgc ttttaacatc tccagtgcac agctgttgat ttcagcgggc tttttcttgc
    32581 caatcagcag tggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    32641 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgct gaagacagtt
    32701 tgctcatcgg ctgaccgtta aagatcacat cgccggaggt tggcgtatcc agcccgccta
    32761 acaggtgcag caaggtactt ttaccggaac cagagctacc gacaattgcc atcatttcac
    32821 cttcgcccac gctgaaactg acattgagca gcacatcggt ttgcacactg ccttcctgat
    32881 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    32941 ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    33001 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
    33061 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    33121 agcgccgctc cgaggattgc accgataatc ccggcgctag ccccttgcac cataaagacc
    33181 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgcgacttc gccctgcttc
    33241 tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacagc gacaatcagg
    33301 ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    33361 cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    33421 actctcagcg gctcatccag ccacaaacgc cagccggtaa tattggctgc cggataacgc
    33481 atcagacgcg aggcatcctc aatattcacc agcatttcat agccatcgac ttcactgttg
    33541 gcggcgaaag tgccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
    33601 aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattgac gcccaactgt
    33661 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    33721 acattgacca gatacggcgt tagtggatct ttttgcgccg ggtcgatacc gagcatcacc
    33781 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    33841 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    33901 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    33961 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    34021 atggtagaaa gccaggagac gaaacgaccg aagcgatcca ctgcacgccc acgcatgtaa
    34081 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    34141 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    34201 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatattt tatcatcaat
    34261 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    34321 gggtcataac cagcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gttctgatga
    34381 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    34441 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    34501 tcctgagcaa atgctggatc tatttcaatc tttaccttgc cccctttcgt atgccggttt
    34561 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    34621 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    34681 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    34741 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    34801 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    34861 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    34921 ccacgccgtg gtggggaatg aacagtgtga tacgcaattt gccttattac agccttggcg
    34981 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    35041 tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
    35101 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat
    35161 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
    35221 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
    35281 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
    35341 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
    35401 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc
    35461 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    35521 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    35581 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    35641 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    35701 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    35761 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    35821 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    35881 cagcgtggca tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    35941 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    36001 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    36061 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    36121 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    36181 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    36241 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    36301 gaacatattt accagcaagt gagtaaaggc acattacctg ctgggatcga gtactggcag
    36361 ccattgttct tcagcgaacc actgccaccg ctgttcagtt atttccctgc caataccttg
    36421 ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaggctga cacgctggcg
    36481 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    36541 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact
    36601 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    36661 gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    36721 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    36781 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    36841 gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggc gcgtaatctg
    36901 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    36961 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    37021 ccggtggtcc atctggagca cggcgtcggt cgctatgccg gaatgaccac gctggaagct
    37081 ggcggcatta ctggcgagta cctgatgctc acctatgcca acgacgccaa actgtatgtt
    37141 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    37201 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    37261 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    37321 gcgtttaaac acgatcgtga gcagtatcag ttattctgcg acagcttccc gtttgaaacc
    37381 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    37441 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    37501 gctttcctgg cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt
    37561 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    37621 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    37681 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    37741 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    37801 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt
    37861 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    37921 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    37981 atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac
    38041 attcagaaag ccgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt
    38101 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    38161 ttcaacgtgc tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac
    38221 accattatca ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    38281 cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    38341 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    38401 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    38461 gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    38521 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    38581 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac
    38641 acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    38701 atcaaagtcg agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg
    38761 gatattgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    38821 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    38881 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    38941 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    39001 ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa
    39061 catttacaaa ttctttgcac tttcctgcac tatccgacac tgtcaccatc cataattcag
    39121 gcttatctgt ttattacaat aaccttatat ttattatgta tttttggtga tgatcaaaac
    39181 gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc
    39241 ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa
    39301 atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt
    39361 cggctttctc gctctgttac aagctaaccc cggcgttgat ccttacgtac cgcgcgcggg
    39421 cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt
    39481 gatcaacatt gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt
    39541 gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac
    39601 cgtttcagac aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta
    39661 taaagcacag ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg
    39721 ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc
    39781 cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat
    39841 caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat
    39901 aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga
    39961 gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt
    40021 taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg
    40081 gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa
    40141 aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg
    40201 agggttaatg cttatttata aattactgct gttccatgga gggtattcgg accggttaca
    40261 gaagtaatac ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc
    40321 agcgatccca gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct
    40381 ggcgttgact gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc
    40441 gcagcgatga ggtttttacg tttttcataa tagtggcctt atgcagatga atgacgtcgc
    40501 aagaaattac ttaacgatcg ataactaaat gatagatgtg atctggatca catacaagat
    40561 attttttata acaatcatta attaatttaa aaatctttaa atttcagtca aatagaaagc
    40621 aattattttt atgggatctg gactggtgaa cggaggtgat tatttttata ataacctttc
    40681 attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa
    40741 aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt
    40801 gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag caaccggagc
    40861 caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc gtgccgttct
    40921 cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa
    40981 aagcgtggag tctgcgctgg ctgactattt tgctgccatc gccaactgct ttaccagcaa
    41041 agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc cagattcagg
    41101 agatatagcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt
    41161 tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cacatctggc
    41221 agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct
    41281 ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac tggtgaaata
    41341 agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc caaattgcag agtacagcct
    41401 tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat ctgcgtaccc
    41461 ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa
    41521 ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa
    41581 gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc acccagcgcc
    41641 cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca
    41701 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
    41761 tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
    41821 gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact
    41881 gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc
    41941 acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct
    42001 atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc
    42061 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
    42121 gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt
    42181 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
    42241 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
    42301 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
    42361 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
    42421 accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
    42481 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
    42541 gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
    42601 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
    42661 taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
    42721 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
    42781 cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
    42841 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc
    42901 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
    42961 cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg
    43021 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
    43081 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
    43141 acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
    43201 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
    43261 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
    43321 cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
    43381 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
    43441 gacaatcaca atctttttca atggcgtagt caacgtgccc cccttaaatt tattaaccaa
    43501 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
    43561 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
    43621 caagttttgt tgcttaaaag ataggcaact ttaccggggg agatcaaaaa atagagttta
    43681 ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat atcgagggcg
    43741 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    43801 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    43861 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    43921 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    43981 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    44041 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    44101 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    44161 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    44221 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    44281 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    44341 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    44401 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctacttt
    44461 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    44521 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    44581 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    44641 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    44701 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa accccagttc
    44761 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    44821 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    44881 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    44941 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    45001 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    45061 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    45121 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    45181 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    45241 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    45301 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    45361 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    45421 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    45481 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    45541 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    45601 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    45661 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    45721 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    45781 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    45841 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    45901 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    45961 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    46021 ccgcaccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    46081 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    46141 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    46201 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    46261 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    46321 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    46381 ccagaggcgc aggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    46441 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    46501 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    46561 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    46621 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    46681 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    46741 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    46801 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    46861 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    46921 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    46981 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    47041 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    47101 cggtaacggg ttcttgcctt tcgttataaa tcgttgagga tgctgaaggt tgaatatcag
    47161 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    47221 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    47281 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    47341 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    47401 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    47461 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    47521 cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg ttggcgcttg
    47581 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    47641 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    47701 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    47761 ttaaacctgc tctttcaatc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc
    47821 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    47881 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcag caaaattatt
    47941 cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg acaacataaa
    48001 ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat caagtcatca
    48061 ccaaaccgtc actacttgcc ggttgcatag cactggcact attaccttct gccgcttttg
    48121 ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct ccagatgatg
    48181 gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag atgactcaac
    48241 atgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat cagcagttac
    48301 aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag gcggattccg
    48361 atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt gatggtatcc
    48421 ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg gcactgtttg
    48481 aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt aatccatctg
    48541 cggcaattaa tatggttcga aaacacgcga cctgtcgtga atttaaaggc gatgtctcgg
    48601 cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc ccactcaccg
    48661 aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac tcatggctgg
    48721 accgctacaa tagtgaaaag accttcttct cgggcattgt cgatgctgat ttaggcgatc
    48781 ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc ccaacctggg
    48841 gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgc gcacgcagta
    48901 ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg accctgaagc
    48961 agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa gtcgaatttg
    49021 acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg ctggttgggc
    49081 catacagtaa ttatggacct ggctttgatc atgtcggcgg caccggttgg aacagtggca
    49141 aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag ttatgaattg tttggtcgtc
    49201 agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttca gttcatgggc
    49261 caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt tcccacaagc
    49321 cgactggtca tcacagagcc tggcgcagga cgataccaca catatgaaat cgttatatgc
    49381 tgccactcgt gtcacccttg ccgatccgct gcatctgatc ctcggcgcac gttataccaa
    49441 ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc cttacgctgg
    49501 tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct ctattttcca
    49561 gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg gtaacaacta
    49621 cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt tagccatctt
    49681 ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg gcagcaacgg
    49741 cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat ttgaactcaa
    49801 cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctatc ttgcagagga
    49861 taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa tgttcaccag
    49921 ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact ggcaaaatcg
    49981 cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag gtagctacgc
    50041 gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac aggggaacgt
    50101 caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg tctacggcgc
    50161 accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg taaaaaaggc
    50221 agccatctgg ctgccttagt ctccccaacg tcttacggat tagtggttac ggatgtactc
    50281 atccatctcg gttttcaggt tatcggattt agtaccgaaa atagcctgaa caccagaacc
    50341 agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca ctttagacac
    50401 atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa tgttttcttt
    50461 accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg cttttgcatc
    50521 ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca gcacgcggaa
    50581 gatggtgtag taaacaatcg cataaccgat accgacgatc ggtaacagcc acagtttgct
    50641 gctgttacca gacagaacga tgaagtcgat cagaccgtgc gagaacgacg taccgtcacg
    50701 catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt ggatgatgta
    50761 caggatcggc gcaacgaaca tgaagaagaa ctcgatcggc tcggtgatac cggtcaggaa
    50821 cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt ctggtttagc
    50881 agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca ggaagccacc
    50941 agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc cgtggaaaac
    51001 ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt tccagatgtg
    51061 gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa acgcaactac
    51121 cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa tcggcggcca
    51181 aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga tcggcacaaa
    51241 gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga aacggttaaa
    51301 catgtacgct gcaatcgcac cggagataat ccctccgagt acgccagtat ccgccaagtg
    51361 tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca cggccatggt
    51421 tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat cgttattggt
    51481 aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga cggaaccgcc
    51541 tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg cggaaccgac
    51601 gcccagcaga atacctgcga taggtagtac ggataccggc agcatcagcg atttaccgac
    51661 cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg agtatgggtg
    51721 ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac agggcatcta
    51781 agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt tgacggctat
    51841 cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat atgtcacaat
    51901 atttcaaacg cggttattca ccgcccaatt ttcggcggtg aactttactc gttttagcca
    51961 ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg aagcgtcgat
    52021 gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa cggcaacacc
    52081 tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat tctcttttcc
    52141 tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc gatccagggg
    52201 gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa tgccggaaaa
    52261 ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct ctgtaaaaca
    52321 gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca gcgtatcggc
    52381 acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa tctggatatg
    52441 atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat aatccagccc
    52501 ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat cttctacatc
    52561 gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt tgtcacgttc
    52621 gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg ccagacaaaa
    52681 tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat gcaaagattc
    52741 ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt ctctcttaaa
    52801 gatgaggaac cggtaacaca acgcccggtt gcaggtaatg ctcaatacgc agtaaaagat
    52861 cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca cgaatgtggc
    52921 aaacatcccc cagtatagcc tgcaggcacg agggagaaag atggtttgcc agttcggcga
    52981 ccaggcccgg cacatcaaca ttggtcacct gcgcagcgcc atgatggcgt tttagcgcat
    53041 ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga ttaagggccg
    53101 ccagcagcga ataccagtcg cccgatggca cgctatatgc caacgcctga cacaatgttt
    53161 cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc gaaccggcgc
    53221 ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt gaaagccaag
    53281 tcacggcgta ctgttctgga ggcggcgcaa ggtaatgtaa ccgacaacga ctacgtaatg
    53341 ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct gctggtggct
    53401 cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca gcatcggtta
    53461 cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg gtgacctcac
    53521 gtaccgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg tagtaatcgg
    53581 gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt ttgtggccct
    53641 gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca tcgcccatgc
    53701 ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag ctggctacca
    53761 gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact ccttcaccca
    53821 gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg tggcatcaat
    53881 ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg tgcgattaaa
    53941 gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg cgcgttttaa
    54001 gccaacttcc ggggtaacat cgagatagag cgttaagtct gggcgaaaat ccccgagaac
    54061 agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc cctgatacgc
    54121 ctgagtggaa agatcgtggc gatcgccaat cacccaggtg ccgttagcca gcgcaggttt
    54181 gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt cggctttatc
    54241 ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta acttttcggc
    54301 aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca gttgctcgag
    54361 cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc cctcaatgac
    54421 gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc acagacttgt
    54481 tatgactggc aagattggta ttaaacgtgt gaccaccttt accatcggcc acaaaataga
    54541 gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc ggcgtagcta
    54601 tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc gtttccaggt
    54661 ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg gttgggtcgg
    54721 tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc tgatcgcgtt
    54781 cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc tgatttttat
    54841 ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct ttcaccattt
    54901 tcttgtgaac tcgcttgagt aacgcgacat cggtggtatt ggcggtatac atcaaggtgt
    54961 ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc tgcgctacgg
    55021 tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc aattgcttga
    55081 ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct tctttaccgc
    55141 tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg
    55201 ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc cgtgggcgat
    55261 tgatgatctt atcagcataa agctgttcac cgagcgccag acgtccggtc cctggcttca
    55321 gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg cgaaccttcc
    55381 agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata atcaataaca
    55441 cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat ataacgttgc
    55501 tgacgaaaag gtgacatcgc cacaggcacg tacgggcatc actggcatta acgcattaca
    55561 aataaccatc tcatctgcct gcaacgcctc ctccagcgag gcttgcactt cgacaagctg
    55621 ataagaggat tgtgccagta aacggataca gaattgtcgc ataatgccgt taacacctgc
    55681 ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat tagccgcaca
    55741 gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc
    55801 aagatgagag cgaatcaata cttgctcaag ccgattaaga tgtttaatac ctgcaagatg
    55861 aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg
    55921 gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc gttgctggtc cgctgttcaa
    55981 tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca caccattttg
    56041 ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt cacaggaaat
    56101 catcaaccgc tgacaagcat cctgtagtcg caggatatgc gccgataaca aactgacttt
    56161 accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg cccgatcgct
    56221 tactgccagc gattcctgct tataaccgtt aattaagaac ataccggctc cttatcagag
    56281 aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc taagaaaaaa
    56341 ggcccgcaag cggacctttt atatgggtgg aaaatgacaa cttagatctt tttaaagatc
    56401 aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc cattccgctg
    56461 acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg gttatccagg
    56521 ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat agattctact
    56581 gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac caacacacgg
    56641 cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc gccagccggc
    56701 gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc tgcatcacgc
    56761 agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga cgtcatatga
    56821 taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc accgcgtttt
    56881 ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag tacgaagcca
    56941 tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg ggtagataat
    57001 gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc tgcgccacct
    57061 gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc aatgttgtgc
    57121 acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc atacatgata
    57181 gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg gctgatctta
    57241 cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag gccgccaatc
    57301 ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc aaggccagaa
    57361 tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc catcttgcgc
    57421 tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc agcaaatttc
    57481 gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg accggcaagc
    57541 agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc cagtccggtc
    57601 acaactacac gacgcttaga cacgtttgtc ctccaaggag ggaaaaatga ttctagtggg
    57661 acaaaaagat aaaactcagg cggtcgaacg accgcctgga gatgttcact tacgcctggt
    57721 ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat tttctcagct tcttcgtccg
    57781 gaatctcagt atcaaactct tcttccagag ccattaccag ctcaacggtg tcaagagaat
    57841 ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc ttaacgccca
    57901 gctgttcgcc gataattttc ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc
    57961 tatcaaaact cgctttcgcg atggttttcg tagtgtataa aatgttgaaa aatttgcaac
    58021 taaatcccgg caggtcttac cacgatttta cgttattttg aggccttacc ccccaataac
    58081 gcaaatcatt ttgcactaat tattactcat aaccacgcag actgcgcgca acatgagcat
    58141 tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa gtttcacccg
    58201 tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc gttggcgatt tcctgtgcgc
    58261 cgccgaggcg acccgcagga acctgcgcca ggatacccgc acgctggtca tcgctcagcg
    58321 cacgtgtcat gtccgtttca ataaagcccg gagcaacaac gtttacagta ataccgcgtg
    58381 acgcaacttc gcgcgccagt gatttactga agccgatcaa gcccgctttc gccgcagcgt
    58441 agttggcctg accgccattt cccatggtac caaccacaga accgatagtg ataatacgac
    58501 catgacgctt tttcatcata gcgcgcatta ccgcttttga cagacggaaa acagatgaaa
    58561 ggttagtttc gataatatcg ttccactctt catctttcat tcgcattaac aggttatcac
    58621 gagtgatacc ggcattattg accaggatat ccacttcacc aaattctgcg cgaatttttt
    58681 ccagaacaga ttcgatagat gccgggtcgg tcacattcaa catcagacct ttgccgttgg
    58741 cacctaaata atcactgatc gcctgagcgc cattttcact ggtcgcagtg ccaataactt
    58801 tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg
    58861 ttaccagtgc gatttttcct tcaaaattca tgattttcct cttttaaagc tcgagcgccg
    58921 ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg
    58981 tcaggccagt aagcaccttg cccgggccga cttcatagag atgttctacg ccttgcgctg
    59041 ccatgtactc aacagacttc gtccactgaa ccgggttata caactgacgt accagtgcgt
    59101 cacggatggc atcaccattg gtttcgcatt tcacatcaac gttattcaca acagaaactg
    59161 ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag tttgtcggct gctggtttca
    59221 tcagcgcaca gtgagacggt acgctcactg gtaacggcag cgcgcgtttt gcgccagcag
    59281 ctttacaggc agcgccagca cgctcaaccg cttctttatg accggcaata accacctgtc
    59341 ccggagagtt aaagtttacc ggagaaacga cctgaccttc tgcagcttct tcacacgctt
    59401 tcgcaatagg cgcatcatcc agaccgatga ttgccgccat agcgcccgtg ccttccggta
    59461 cggcttcttg catgaactta ccgcgcatct caaccagacg caccgcatca gcgaaatcaa
    59521 tcacgccagc gcaaaccagc gcggagtatt cccccaggct gtgaccggcc atcattgccg
    59581 gtgctttacc gccctgctgc tgccatacgc gatacagcgc aacagatgca gtcaacagcg
    59641 ctggctgggt ttgccaggtt ttattcagtt cttcagctga cccctgctgg gtcagcgccc
    59701 acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca attggatagc
    59761 tcgccgccat atcagccagc attccaacgg tttgagaacc ctgtccaggg aacacaaatg
    59821 caaattgcgt catgttttaa tccttatcct agaaacgaac cagcgcagag ccccaagtga
    59881 atccaccgcc aaaggcttca agcagaacca actgccccgg cttaatgcgc ccgtcgcgta
    59941 cagcttcatc cagcgcgcac gggacagagg ccgcagaggt attaccgtgg cgatccagcg
    60001 tcaccacgac attgtccatc gacataccga gttttttcgc cgttgcactg ataatacgca
    60061 ggttagcctg atgcggaacc agccagtcca gttgagaacg gtcaagatta ttcgccgcca
    60121 gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac cttgaagact tcgttgcccg
    60181 ccatcgtcag atgaattgaa ttctctggat tcacgcggtc ggcgtttggc agcgtcagca
    60241 attcaccata actaccgtcg gcatgcagat gggtggaaat gattcccggc tcttcagagg
    60301 cagccagcac cgcagcgccc gcgccatcgc caaaaataat aatagtccca cgatctgttg
    60361 gatcgcaggt gcgcgccagt acatcggaac cgacgaccag agcatacttc accgccccag
    60421 atttcacgta ttgatcggct acgcttaatg cataggtgaa acctgcacag gctgctgcaa
    60481 cgtcaaatgc cgggcaacct ttaatgtcca acatgctttg aatctgacaa gctgcgctcg
    60541 ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag gccaatctgg tctttctcaa
    60601 tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt
    60661 ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt gacaatccac tcgtcagagg
    60721 tgtccaccat tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga tagctgccag
    60781 taccaataat cttcgtatac atgtacgctc agtcactttt cggttatata ccgtcacttg
    60841 caaactgcga gttcgctggc agcgtcctgc taccgcagag ttccgctttt gccaccgtcc
    60901 agcagctcaa aaccagctgg gtatacagat tccaggcgag cggcaattcg ctgaggaact
    60961 tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg ctgattggct
    61021 gcaccatgac tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg
    61081 tcggggttga ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg ctttaataac
    61141 agtagccacc acgaccgttt tttcccttca ccctgagatt tcagcagaga aaggaacatc
    61201 ctgacaacac cttccatcgt ctttaacgtg acatttcctg taaagccgtc acaaaccagc
    61261 acatctgtct tgccagttaa caactcattg gcttcaagat agccgatata attgatagaa
    61321 gggattgttt taagcaccgc tgaggcatcc cgaatactgt caagaccctt tacttcttct
    61381 tcaccaatat tgagcaacgc cacgcgagga ttgggaattt ccaccacctc ttcagccaga
    61441 actgagccca taatggcaaa ttgcaccaac attgtgctgt cacaatcgac gttggcccct
    61501 aaatcgagga ccaccgtttt gcccttttgc tgatgtggta ataccgtcac cagcgccgga
    61561 cgctcaatcc cctccagggg cttgagtaat aattttgcca gccccatcag tgccccggta
    61621 ttaccggcac tgacacaggc ttgcgctcga ccttctttca ccagctccag ggccatacgc
    61681 attgaactcc cacggctggc gcggatagct tgcgaaggcc gggcatcact ggcgataact
    61741 gactgcgcag gaataatctg cagacgcgaa cgttgttcaa agtcagcttt agcaagtaat
    61801 ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga gttgcgaatt agaattcagt
    61861 gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat cccctcccat gacatctaac
    61921 gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct
    61981 ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga ttacttagcg atgaccttgc
    62041 ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt
    62101 tgtctacaga caggctggtg actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt
    62161 tggaacgggt tggtttattc tgttgtacgg ccatggacct tactcctcaa ttacggttta
    62221 tccccgatcc aacggggagc accaattact tacgctttaa gctggctaat acggcaaatg
    62281 ggtttggctt ttgcacttct tcaggcagtt caccaaagac catgtccgct tcggacactt
    62341 cacagtgttc agaatcatgc accggaacta ccggcaaggc gaggatgatt tcatcttcaa
    62401 ccattgcaag cagatcgatt tcaccgaatt cgttaacctc aatcggttca tacgcttccg
    62461 gcagtgcttc agcctgttcg tctgaacgca caggactaaa acaatacgtt gtgtagacct
    62521 gatgagtaaa cggcttcccg caacgctgac actcgagcgt taccgtcacc ttcgcatcgc
    62581 cgtttaacac tgcgagacgt tggttatcga tagcgaacga catggagcat tccacatcac
    62641 tgtccacact gactacggat tcggcgacgc gctcaacctg atcaggggta tagataccct
    62701 ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag agtcaggggt aattttacct
    62761 tttgcatagg gcgcgcatat taactttgta acgtcataga gtcaaagaaa aaggcagcct
    62821 gtggttgcct tttgccaata attcgcacac attgcgggtt actgctttat tttcgttcag
    62881 tgctgataac gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa
    62941 tacgctatat ctgatggaaa aaaatatgcc taaacttatt ttagcctcca cctcgccatg
    63001 gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac ctgaggtcga
    63061 cgaaacccca cgcagcgacg aatcaccgcg acagttggtg cttcgactgg cacaagaaaa
    63121 atcgcaatct ctggcgtcac gttatccgga tcatttaatt attggttcag accaggtatg
    63181 tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa gaaaatgccc gtctgcaatt
    63241 acgcaaagcc agcggcaata tcgtcacctt ctatactgga ctggcgttgt ttaattcggc
    63301 gaatgggcat ctacaaacag aagtggagcc ttttgacgtc catttccgtc acctaagcga
    63361 ggcggagatt gataattacg tgcgtaagga gcatcccctg cactgcgcgg gtagctttaa
    63421 gagtgaagga tttggcatta cgctgtttga gcgtttagag ggacgtgacc ctaacacgct
    63481 ggttggttta ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga aaaacccgct
    63541 gatgggatag ttgtttgcgc cggatgcggt tacgtcgcat ccggcaatcg gtgcaggcgg
    63601 cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat tatcaggctt atatgcttgt
    63661 attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa accttcatcc atcggcgctt
    63721 cgatacgcat cacctcaccg gtccccggat gggtaaactt caacgctgcg gcgtgcagga
    63781 acagacgatt taatcccgtg cctgcttcag tgagctgtct gtcaaattca cggtcaccgt
    63841 agcgatcgtc aaaggcaatc ggatgacccg catactgtgt atgcacacgg atctgatgag
    63901 tacgtccggt taccgggcta caacgcacca gggtggcaaa tgcatagcgt tcttccactt
    63961 taaagcgtgt ttccgacggt ttgccttcct gactgacacg cacgatacgt tcgccgcttt
    64021 gcagaatatt tttcaataac ggcgcttgaa cgctcttcac atgcgactgc cactgaccgc
    64081 gcaccagcgc caggtaatct ttttgcattc ctttttcacg taattgctca tgcagagaac
    64141 gcaacgccga gcgttttttc gctaccagca acacacctga agtgtcccgg tcgagacgat
    64201 gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg ataacgccga
    64261 agcttaaacc actgccgcca tgtaccgccg taccggaagg tttattcagc accaggatat
    64321 gatcatcttc atataagatg acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa
    64381 ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat acgcacctca tcacccgctt
    64441 cgagtttata ttcaggctta atacgttttt tgttcacccg cacttcacct ttacgcaaaa
    64501 tacggtaaat catacttttt ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac
    64561 gttgccccgc ttcgtcagcg gtgatagcaa caatttttac ggatggagtc tctgttttca
    64621 tggtgggcga ttctaattag ccaacaggat tcgcgccact catttttcta tgcttatttt
    64681 tagttttgca ccttattact tcactacgtg atcactttat tgatggttat taaaccaatc
    64741 accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
    64801 tataacaagg cttgcagtgg aataatgagg ccgtttccgt gtccatcctt gttaaaacaa
    64861 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
    64921 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
    64981 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
    65041 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
    65101 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
    65161 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
    65221 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
    65281 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
    65341 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
    65401 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
    65461 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
    65521 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
    65581 atgccaaata acccgcgcgc aggtggcatt tctcgccgta tcgaaggcga tgaccgtacc
    65641 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
    65701 accgctggcg taggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgcctgaaa
    65761 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ctccgttcct gattcatcag
    65821 gagagcaacg taatcgttcg cgccttccgc gattatttac gtcaggatat cggcgaaatc
    65881 cttatcgata acccgaaagt cctcgaactg gcacgtcagc atatcgctgc attaggtcgc
    65941 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
    66001 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc atccggtggt
    66061 tccattgtta tcgacagcac cgaagcgtta acagccatcg acatcaactc cgcacgcgcg
    66121 acccgcggcg gcgatatcga agaaactgcg tttaacacca acctcgaagc cgccgatgag
    66181 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
    66241 atgacgccag tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
    66301 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgc
    66361 cagcgcctga gcccatcact gggtgaatcc agccatcacg tctgcccgcg ctgctccggt
    66421 accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgttt gatcgaagaa
    66481 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtgcc aatcgcttct
    66541 tatctgctga atgaaaaacg ttctgcggtg aatgcgattg aaacgcgtca ggacggtgtt
    66601 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gttgcgcgtg
    66661 cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc cgaagctgca tgaagaagcg
    66721 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca acctgcactg
    66781 gcaacctttg ccatgccgga tgtaccgcca gcgccaaccc cagctgaacc tgccgcgact
    66841 gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag cagctcctgc acaacctggg
    66901 ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtggtga agaagccaaa
    66961 ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
    67021 aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
    67081 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcaa
    67141 actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc
    67201 gacgagcagc aagtgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
    67261 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
    67321 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
    67381 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
    67441 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
    67501 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
    67561 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
    67621 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
    67681 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
    67741 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
    67801 atggtgactg aggtccctgt cgccgccgct gtcgaacctg ttgttagcgc gccagttgtt
    67861 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
    67921 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
    67981 accgagtctg atgttgccgt agcccaggaa gtcgcagaac acgcagaacc gatggttgaa
    68041 ccgcaggaag agacggcaga tgcagatatt gaagaagttg ccgaaactgc tgaggttgtg
    68101 gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca
    68161 gaagttgaaa cggtggccgc ggttgaacca gagatcaccg ttgagcataa ccacgctacc
    68221 gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac
    68281 tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg
    68341 catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaaagt
    68401 aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa catattaaaa
    68461 ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa atgcccgccc
    68521 cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag
    68581 cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    68641 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    68701 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag gaatccagcg
    68761 actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca ttattcagcg
    68821 agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct ttatccgctt
    68881 cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat
    68941 tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa
    69001 ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt tgcttaatac
    69061 tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc tctgttttat
    69121 atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc acgaagcccc
    69181 tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt gctggcgtag
    69241 acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac actctcttcc
    69301 agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt ttgcgcctgt
    69361 gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg gttaacgact
    69421 cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga attggtgatg
    69481 ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc tccccttagc
    69541 gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca
    69601 ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg acaccggaaa
    69661 tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg ctactggttt
    69721 tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg tcgttaaagg
    69781 atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc tgaccgttgc
    69841 ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct tcgtcggtga
    69901 ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag ctgtcgttaa
    69961 cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta ccgttggcat
    70021 ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac tgattattat
    70081 cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc gtggcaccga
    70141 tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg atagtaaaga
    70201 aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg ttgaaagcct
    70261 cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga tcctgagaac
    70321 ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg atctcaatat
    70381 tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg ctggaaggaa
    70441 ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg gcatcgtgat
    70501 gttataagta ccgccatcct gaacgctgac ttctacgcca acaatctggt ttaattcgct
    70561 caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt
    70621 caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt tgatctgatc
    70681 aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt
    70741 ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc gcgccgccgg
    70801 gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc
    70861 cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc
    70921 ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg ttggtaataa acgtatcata
    70981 ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt
    71041 gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga
    71101 gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt
    71161 attgatcaag ctggacatgg aggttcctta taagccttca attacagagt attatcggca
    71221 gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg
    71281 ctcaccttgt cgcttatcga tttcatctgc tggatcatgt tggtgagttt acgggcatag
    71341 tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc cgcactcgcg
    71401 gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta atccgacaat
    71461 gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc ttcgccgttt
    71521 tcatattcag tcgtggtgat ttcagtgact tgccctttcc agttgccaga ggctttgaca
    71581 ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg cccccaacca
    71641 gattcaagcg cagcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc
    71701 agttgggcgg gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg cagcgaatca
    71761 tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt ttgataacgc
    71821 accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt
    71881 tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc
    71941 atctgttggg caatttgctg gtcatacata ctggtataca gacgagtgtg ctcgctgctg
    72001 aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc
    72061 ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct cgctttcgcc
    72121 tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg
    72181 atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcaaaa
    72241 tagacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc acgttattga
    72301 ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg
    72361 tttgtggagt aacaacagtc tgtccaccac caaacggtgt atctggctgg ctgacattgg
    72421 cctgacgatt aactgttact gagagatttc cctgcgctac cgcgcagctg tcgagggtca
    72481 cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta gcgtcctgcg
    72541 gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg gaactgttgc
    72601 cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac
    72661 gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt
    72721 gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac
    72781 cattggtgat ccgtccaccg ttcagttggt taacctgaac actgctaccg cctgcggagg
    72841 ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc
    72901 ccttaagcgg agtcatcaac aacgtacctc cacgcaagct tttggcatta cccatggaag
    72961 aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac gctgtcacca
    73021 ttaccgcagc gacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct
    73081 gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccag
    73141 tgccatccag ccccactacc aagccatagc caatcagtga gttttgcctt accccctgaa
    73201 cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt agaagaatta
    73261 atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa
    73321 acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat
    73381 acgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt
    73441 aaccacgccc gagaagcgaa taaattcggt accctgatta atggcaatct gtttttcacc
    73501 caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct
    73561 aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac cggaggcttc
    73621 gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag tatcaaagcc
    73681 aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt
    73741 ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag
    73801 cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt tggcgacggg
    73861 cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg tggagggtat
    73921 ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc
    73981 gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta
    74041 tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt
    74101 tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt
    74161 tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt
    74221 cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagtc
    74281 cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc
    74341 cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg
    74401 gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc ccgttctgat
    74461 ccacctggaa agagccgtca cggatatagg ctgatgagcc atctggcaac attacctgga
    74521 aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtctgcgac agattgccct
    74581 ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt aatccggagg
    74641 gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga taaagcagat
    74701 cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc aggttgttgg
    74761 caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc cataatgaac
    74821 tgatcatata atgtcctgta gtggattgct gacaagcaga gcagaagcac gcgacagtgt
    74881 gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaatagtcc gcgcgctttt
    74941 attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg cacggcctgc
    75001 gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg cgctggcaat
    75061 catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc ccgacatcac
    75121 acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt ctgcgcttaa
    75181 acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca ccagtttcag
    75241 acgccctact ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg agattgtgcc
    75301 atcggcagca atcgtgattt ccgccccttc cggcacagca attggccccg cctcgcctat
    75361 caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc tgccattacg
    75421 cgtataccct tcgctgccgt cagcggtctg cacggccagc catcctgctg caacgcaacg
    75481 tccagcgggc gagaggtgta atccatcttg ccgggcgtca tgtctgcacc cggtgttgac
    75541 gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc gcgcaaggcg
    75601 tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc ggttaccgcc
    75661 tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg atccattgag
    75721 ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg aggatctggt cctgggtttt
    75781 gatggtctgg gcgttagact tatagttacg ctgggcaacg atcatattga ccagttcttt
    75841 actgagatcg acgttggacg cttccagcgc accgttggtc agagtgccaa agtttcccgt
    75901 cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc
    75961 ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg
    76021 ggtttgttcg ttggaattgt tgccgacaac cgtaccgtca tcattgattt gataactcac
    76081 cagatcgccc ggtttgtagc cattctgggt ggttgccaca atgttgttag cgccggttgc
    76141 gatattattc gccatcgcac catccactaa tgtgccatta gcattaaatt ccagtgttgt
    76201 cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct gccagttatt
    76261 atccccggtc ttcacaaaat agacgctcat gtcatgagca ttaccctgac tgtcgaaaac
    76321 agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt
    76381 aggaagcgga tcactggaat tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc
    76441 tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct gaatagtcgg
    76501 cggcgtaccg gttaccgggt aacccgtcag ctgtaaacct tgcgtattca acaggttacg
    76561 gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac
    76621 cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt
    76681 cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga
    76741 accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt
    76801 ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc
    76861 ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc
    76921 gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt tgccgcggat
    76981 cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact
    77041 ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag tcaacgaacc
    77101 gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg tgcgcacaac
    77161 cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac
    77221 accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt
    77281 tgtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt
    77341 atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt
    77401 gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg
    77461 gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct
    77521 gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt
    77581 taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg
    77641 cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg
    77701 ttaaccatct ccccgacgac atcaacgttc ggcattttta tgtagccctt tgcatctgcc
    77761 agcggattac ctggttcata aaccagtttg tccggggcct gactttctta acatcggcaa
    77821 cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc acctgttttg
    77881 cccgatatgg ctgtccatca ggaccggtca cgctatcagc atttgccaga ttactggccg
    77941 ccacgttcag gcgctgggac tgggcagtta acgccgaccc ggcgatatca aaaatattca
    78001 gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg atttgcccgc
    78061 tcaacgcgct aaggctcatc tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc
    78121 gatccatatc gacggtatta ccgtcaagcg aaggctggtc cggaatacgg tattgcagtt
    78181 ctgcggtagg aggcgtcagc gcctgcgccg ggatgtgctg cgttgaggtc atcgtcagtg
    78241 caaccacact ggttgcatcc cgtccacgtt gcatgacttt tttaagttca ctggcaaaat
    78301 cgatatcgcg cgcctgataa ccaggggtat cggcattggc gatgtttgct gccagcactt
    78361 cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct
    78421 tatcgagcat atctcctccg caggtatcaa aattctgcca tcagcttaaa tgcctttacg
    78481 cgcgcgttat cggcggaata aacgcaaaat gggtcgctat ttatgccgtt gatggtcatt
    78541 gcggacaggt acaattcacg ttgtagaaat ggctgggggc gaaaatgctg acaataaaac
    78601 gtagcgtggc gatcatcgcg atactgttca gtccgttaag tacggcgagc aatctcacat
    78661 cgcaattgca caactttttt agcgcccaac tcgcgggggt aagtgatgag gttcgtgttt
    78721 ctattcgtac agcgcccaat ctactaccgc catgcgagca gccattgctt tcgatgagca
    78781 ataattcccg cctgtggggc aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat
    78841 atttacaggt taatgtgcag gccacaggaa attatgtggt tgccgcgatg cctattgtgc
    78901 ggggaggaaa gctggaagct ggcaatgtca aactgaaacg tggacgcctg gataccctgc
    78961 caccgcgtac ggtgctggat atcaatcaac ttgttgatgc cgttagcctg cgcgatctat
    79021 cacccgatca acctatccag ttaacccagt ttcgccaggc atggcgggta aaagcgggac
    79081 aacgcgtcaa tgtgatcgcc agcggtgatg ggtttagcgc caacgcagaa ggtcaggcgc
    79141 tgaacaatgc agccgtcgca cagaatgcgc gggtgcgcat ggtatcggaa caggtagtca
    79201 gcggcgttgt tgatgcagat gggaatattc ttataaacct gtaagctgtt aaagattacc
    79261 cgtcccttgc cgataaataa gcaacacatg ataaaagcgc cctcaatgag gaataaacca
    79321 tgagtattga tcgcacttcg cctctgaagc ctgtaagcac cgttcaaccg cgcgaaacca
    79381 ccgacgcgcc ggtaacgaac agccgggcgg caaaaacaac cgcctccacc agcaccagtg
    79441 tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat atcaatcttg
    79501 aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga actaaaaatg gacaccggca
    79561 aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac tgatcgtatg
    79621 acacgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct caaaacggta
    79681 atggatcaag agcagcaaca tctctctatg gggcagatca acggcagcca gttgcaatgg
    79741 attacagaac aaaaaagctc actgctggcg acgctggatt acctcgaaca gttacgcaag
    79801 aaagaaccca atacagcaaa tagcgttgat attagtcaac gctggcagga aattactgtg
    79861 aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga aggacagatt
    79921 gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac gctatatggg
    79981 gcgaacggtc agacctcaac accccatcgc ggcagtaaaa agatttcgat ctgaatatta
    80041 tctccggcct gcactgcagg ccggaatgca ttgttacacc gtccggcggg caaattcttt
    80101 aactttgaag cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac
    80161 cgccattaaa cgcagtaagc gccagggcat ggtacccaat gaccactccg gcatgatatg
    80221 taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac gcaacagaaa
    80281 cgccatccag ccgggttgcg gagtaaagat tttctgctta cgcaactgcc agtaaagcag
    80341 cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat gtttcaacgg
    80401 accaataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg caattttcac
    80461 tggcgtttta atgtcctggc gggaataaaa gccaggagcc aacactttca ctacaatcag
    80521 gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc
    80581 ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc
    80641 aaccgcactc ggcagtgcca acaggaaaca aagacgcaac ccccagtcca tcaaacggtt
    80701 gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca aaatggtgcc
    80761 aagcgccacg cccagcacac cggagggaaa ctccattaag cggtcggcgt aatacatcca
    80821 agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta aggagatctg
    80881 gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca ttgctccagc
    80941 atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt gcggtagctg
    81001 atacaccagc tgcagaatgc cgccgaccgt aacagcccac gccagcgcca gcaccggtgg
    81061 gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg
    81121 cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca ccagcgacgc
    81181 cagggagatc agcaagatat agggaaaggt aatctttagt agctggctgg tcagggcaaa
    81241 tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg atcacccacg gtgcggcgag
    81301 catgccagcg accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa cataagagac
    81361 aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa tcggtacaaa
    81421 tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa gtttaaaagc
    81481 gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg
    81541 tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca gcgattttaa
    81601 taaattcatc ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg caggcgctga
    81661 aagaggcgaa tagtctacct agattatggt gaattgctac cgccagatgt tacagggttt
    81721 attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc actgcctatt
    81781 cgccggcggt ttgcggaact gtctgatttt acacgcattc aatgaagtgc cgcgcacagc
    81841 cgacaaatcc acgttgctca agagtgctct gccaaccagg aatcggttta ttcactacac
    81901 cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag
    81961 tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacaa gtggtgatct
    82021 gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg tcgttagtca
    82081 gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg gtatccacca
    82141 catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg ctattgctac
    82201 gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac tcaccgtaga
    82261 gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc gccgccagtt
    82321 ccaccagcct ttcagcatcg cacagatttt ctgccagcgg tttatcgaca cagacatgta
    82381 cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg gagtgcacaa
    82441 aaaccgcatc acaactggcg gcaaggctgg ataacgaatc ggcataagga atgcaccagc
    82501 tgtcacaaat tggcagggct ttggcgcgcg taggcgacca ggctccttgt aacgtccagt
    82561 cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct aatcccacta
    82621 cgccgatacg taattttttc actttaatct cccagatggg ccagcaacga atcaagacgt
    82681 tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggtctgtaa atcaccgtca
    82741 accatgttcg acatagccgt taccgccggc tgatcttcaa cctcaccact gaaaagatgc
    82801 atgtagcggc tttcgcgttt acccggttcg cgggccagac gcaccacaaa aggaccatct
    82861 tcgcgattcg ccagttgttc cagcgtcgac tccacttccg ccatatcgct gaattcatac
    82921 attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa taacaacgtg
    82981 gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga attacaaaaa
    83041 cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag ataatgacgt
    83101 ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat gaccggttca
    83161 cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcag gtattgctcc
    83221 ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc gagcttccag ggcagtaagt
    83281 tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag ttaatgccgt
    83341 cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat agccttcttt
    83401 ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca tataattagc
    83461 cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg tcagggcttc
    83521 aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg
    83581 aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg
    83641 gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccctag
    83701 cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct cccagggctt
    83761 gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac gatcatgcac
    83821 cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc
    83881 aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac
    83941 ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat tcacatattt
    84001 gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tactgtcccc gaagtgatgg
    84061 gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga
    84121 aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat
    84181 gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca
    84241 aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt ttattcagca
    84301 aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt
    84361 taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg
    84421 gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg atccgccgcg
    84481 ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt
    84541 gatgatgcag gacagtgcca gtgcggtcat tggcgcattg ttggggagct ggctgttgca
    84601 atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctat gtgcggcgtt
    84661 caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg
    84721 catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta
    84781 ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggctgg
    84841 cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct
    84901 ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg
    84961 gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc tacaacaact
    85021 tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac
    85081 cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt ttagccgtct
    85141 gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt ttgacctggg
    85201 caaatcggcg caccagccag agcttccgtg gatgatgctg ggaattattg gcatcttcac
    85261 tttccttgcg ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg
    85321 cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac tgacagccaa
    85381 atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc tcaaagcccg
    85441 catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca acgacgacgc
    85501 agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa aagatgacag
    85561 ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg acggcaaacc
    85621 gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta
    85681 cgtcaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt tttctactcc
    85741 cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt ttgccgacct
    85801 gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg cgctggacaa
    85861 actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata ttcagctatt
    85921 tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc gcgttgctaa
    85981 ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa tggcgattta
    86041 atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag ttttttctgc
    86101 tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac gcagatttca
    86161 tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc agtggcctgc
    86221 tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt aaccagtcgc
    86281 ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct gacgcccata
    86341 ttgttctgac aaacttgacc agccaaattg gtcgcgaaga gccgaataag gttaccctaa
    86401 ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg accatgaaac
    86461 tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga
    86521 tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc
    86581 ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct tacgtcctgc
    86641 gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga
    86701 agccaggcga aattgtcatt ccgtttacgg attaatgacg agggcgcatt cgcgcccttt
    86761 atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa aacatccctt
    86821 cagccggagc atagagatta atgactgcac catcccaggt attaaagatc cgccgcccag
    86881 acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg ccgtatacca
    86941 gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg accacagttg
    87001 aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt acctgccggg cacgatttca
    87061 ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg gagcgcggat
    87121 ttaatgaagc cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc actaactcca
    87181 gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg
    87241 gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg
    87301 aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag
    87361 tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac ggaaatgaac
    87421 ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg
    87481 gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt caccaacagg
    87541 cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg gattctgcgc
    87601 cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc aacgccccaa
    87661 ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag cactttgaag
    87721 cattctgttc tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac acattcatcg
    87781 aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat gacaccctgg
    87841 tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa tcgtagcttc
    87901 ctgttgtcat taggttattt tacctgtata aataaccagt atattcaaca gggggctatt
    87961 atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc tattgacgcc
    88021 ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga aggccacgta
    88081 tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcaacaaa agaagataaa
    88141 cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc
    88201 gaataatatg cagtgatttt tgtttttgcc ggatcgcccc ggctttttta ttttacttac
    88261 gaattttgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat gctaaaaaaa
    88321 tatgaatgac atgctgtttt attgttcata taaatcttaa aagcagaaat ttatctgttc
    88381 ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt gcttcagata
    88441 aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg ccatgatgga
    88501 aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca gcaccgttga
    88561 tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt ccgagcagga
    88621 agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac aatttatttc
    88681 gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa acgagtatcg
    88741 gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc ctttaccatt
    88801 tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga gcagagagaa
    88861 aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg ccgccgggta
    88921 ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac gcccatatgc caccgcatca
    88981 acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg aaggcgaaaa
    89041 gtatgtcccg ttggtcctcc gcgcgcaaac gctgtgggat gaactttccc gccacaacga
    89101 agatgatccc atttttgtac gctctggtgt cattaacctc ggcccggctg actccgcatt
    89161 tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc aacgttgaaa agctcgatgc
    89221 gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca tcggcttatt
    89281 tgagactgat tccggttttt tgcgcagcga actggcgatt aaaacctgga tccaactggc
    89341 gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc accgcaattc gtcatgacga
    89401 tgatggcgta actattgaaa cggttgacgg tgagtatcag gcgaaaaaag cgattgtctg
    89461 cgcgggaaca tgggtaaaag acctgctccc ggagctgcct gtccagcccg tacgtaaagt
    89521 atttgcctgg tatcaggccg atggccgcta tagcgtgaag aataaattcc cggcgtttac
    89581 cggtgaactg cccaatggcg atcaatatta tggttttccg gcagaaaacg acgcgttgaa
    89641 gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg ttccgtttgc
    89701 ggaagtggcc agtgatgggt cagaagcctt cccgttcttg cgcaatgtat tgccgggtat
    89761 cggttgctgc ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg aagattttat
    89821 tatcgatacc ctacccggcc acgataatac actgctcatt accggcctga gtgggcacgg
    89881 ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag accaaaaaag
    89941 cgattttgat ttgacgccat tcaggctttc ccgcttccaa taatcatcat tacggcctcc
    90001 agaatgaggc cgtttttaca ggagtattta atgcgtcggc tgttgcacta tctcatcaat
    90061 aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc aattatggat
    90121 ctgatctatg tgttttattt ttagttttcc gcactgacat aaactgacaa atatttaatc
    90181 tccctttcat ttagcgtgaa aaattgaatg agattattcc tgcttctaca gcactcacca
    90241 gacatctctg atgctgaacc cactaaactt atgaagattc tttcattatt aattcaaaat
    90301 tattgaaata aaaataaata tcattcaatt tgttattttt gtgttgcaat tcattctccg
    90361 acacgccatt ttctttctct cgcaaattcg tattggatgc acttatgtca ttcacaaata
    90421 cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct
    90481 acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc
    90541 acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc
    90601 gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga
    90661 ctcgtcttgc cgcaaaagct ggacatctcg cattgtgcct attgctgttc gccatcggca
    90721 tcagcggcta tctgatctca actgccgatg gtaaaccgat cagcgttttt ggctggtttg
    90781 acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt
    90841 tttggctcgc gtggagcgtc gtagtactgt ctgttatgca cggatttatg gccctgaagc
    90901 atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact
    90961 atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct
    91021 ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg
    91081 ttaacttccg catacagcat cttggctata gctggttata tggcaccttt aaagatttcg
    91141 acggtacttt tacctttgat gaaaaaaatc cggctgccga taaagtgaat gtgacaatta
    91201 acaccaccag cgtcgatact aaccacgccg aacgcgataa acatcttcgc agtgcggatt
    91261 ttctcaatac cacaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg
    91321 gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc
    91381 tggaagcgaa attaattggt cagggcgacg acccatgggg cggtaaacgt gcaggctttg
    91441 aggccgaagg caaaattaag ctcaaagact tcaatatcaa gacagattta ggtccagctt
    91501 ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg
    91561 ttgccggatg gcacgcatcc ggcaatgata tttattctgt tggatcagga atacccagtg
    91621 ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc
    91681 gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac
    91741 aacctttgta tttttccgcg catactggac actgaataaa cagcaggtgg cagccatcat
    91801 ttttacagtt ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct
    91861 catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca
    91921 ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc
    91981 agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc
    92041 cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg
    92101 gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt
    92161 catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt
    92221 tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa
    92281 taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag tacccagaag gatttcccgt
    92341 catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct
    92401 gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat
    92461 gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac
    92521 gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt
    92581 cgctctcagc caacattttg gcttttaacg cgtcgttgga aatgcggttg tgtaacactg
    92641 gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt
    92701 atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat
    92761 ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa
    92821 tgcgacaata catacaattg cccgtatagg ttgaaaaaca ggattgatat gacgaatcta
    92881 cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc
    92941 gtactttggt tggtcgtgca attgccctac ccgattatct accgcctcgg ttgtggatta
    93001 ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa
    93061 ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa
    93121 tccgttggca tgggcctgat ggaaaccggt atggcgtggt tctggccgga ccgccgaatc
    93181 gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc
    93241 ggcatcctgt tagttggcat ccattttctg acactggagc tgggcgcgcg gcagtttggt
    93301 atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta
    93361 caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc
    93421 atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccagatca tgattacggc
    93481 ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga
    93541 acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag
    93601 ccagatggca aagggtatca attgattatg ctgccaccag agtgttctcc gccactggat
    93661 gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca
    93721 ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca
    93781 cgctattaaa tctcccatgc cggatgcttc agaatggcat ccggcattac cacagcaaat
    93841 ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat
    93901 tagtgtgttt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct
    93961 atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg
    94021 ctaggctgtt ttcttaccgg ggccgccttc agtctggtaa tgcccttctt acccctctac
    94081 gttgagcagc ttggtgttac cggccactcc gccctgaata tgtggtccgg tattgtcttc
    94141 agtataatgg gttcctaaac acatcatttt aggtattgaa tatgagacgg gcaatcagct
    94201 acatcagatt ttcaagtgaa cgacaactga aaggggactc tgtacgccga caatctaagc
    94261 tggttactga ctggcttgat aagaaccctg agttttactt ggattcatcc ttgagcttta
    94321 aagatttggg gaaaagtgct tttagtggta agcacctgaa aggtggtcta ggtgactttt
    94381 taaccgctat cgagaaaggg cttgttaaag ctggtgatac gctactaatc gagagtttag
    94441 accgtctgtc acgtcaggat attgatattg cttctgagct attacgccgt atcttaaggg
    94501 ctggtgtaga tgtagtcaca ctgagcgatg gagaacatta cacacgagaa agtttaaaag
    94561 acccgttagc cctgattaaa agcattctga taatgcagcg ggcgcatgag gaaagtttga
    94621 gaaagtctga gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc tccgaaggta
    94681 tcaaggtatc taggcgttgt cctgcatggt taaggctgaa tgatgaccgt cgaacattca
    94741 caatcattcc tgataaagtg gaagttgtta aacgagcatt tgacctgaga ttacagggtt
    94801 taagcttttg ggctatcacc agaactttaa acgatgaagg tcacttgtca ttgaatcaat
    94861 acacacccaa acaaaagggg tggtctgata ctgctgtaaa gaaactgctc agaaatcgtg
    94921 ctg
//
LOCUS       DAJTRN010000002        93413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRN010000002 DAJTRN010000000
VERSION     DAJTRN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 93413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            92..217
                     /locus_tag="OO328_000106"
     ncRNA           92..217
                     /ncRNA_class="other"
                     /locus_tag="OO328_000106"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            337..678
                     /locus_tag="OO328_000107"
     CDS             337..678
                     /locus_tag="OO328_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5928815.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(669..1595)
                     /locus_tag="OO328_000108"
     CDS             complement(669..1595)
                     /locus_tag="OO328_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5928816.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1685..2683
                     /locus_tag="OO328_000109"
     CDS             1685..2683
                     /locus_tag="OO328_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5928817.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2680..2898)
                     /locus_tag="OO328_000110"
     CDS             complement(2680..2898)
                     /locus_tag="OO328_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5928818.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2900..4915)
                     /gene="ligA"
                     /locus_tag="OO328_000111"
     CDS             complement(2900..4915)
                     /gene="ligA"
                     /locus_tag="OO328_000111"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5928819.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4986..5972)
                     /gene="zipA"
                     /locus_tag="OO328_000112"
     CDS             complement(4986..5972)
                     /gene="zipA"
                     /locus_tag="OO328_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5928820.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6202..6963
                     /gene="cysZ"
                     /locus_tag="OO328_000113"
     CDS             6202..6963
                     /gene="cysZ"
                     /locus_tag="OO328_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5928821.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7148..8119
                     /gene="cysK"
                     /locus_tag="OO328_000114"
     CDS             7148..8119
                     /gene="cysK"
                     /locus_tag="OO328_000114"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5928822.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8503..8760
                     /gene="ptsH"
                     /locus_tag="OO328_000115"
     CDS             8503..8760
                     /gene="ptsH"
                     /locus_tag="OO328_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5928823.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8805..10532
                     /gene="ptsI"
                     /locus_tag="OO328_000116"
     CDS             8805..10532
                     /gene="ptsI"
                     /locus_tag="OO328_000116"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5928824.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10573..11082
                     /gene="crr"
                     /locus_tag="OO328_000117"
     CDS             10573..11082
                     /gene="crr"
                     /locus_tag="OO328_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5928825.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11124..11975)
                     /gene="pdxK"
                     /locus_tag="OO328_000118"
                     /gene_synonym="thiJ"
     CDS             complement(11124..11975)
                     /gene="pdxK"
                     /locus_tag="OO328_000118"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5928826.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12080..12454
                     /locus_tag="OO328_000119"
     CDS             12080..12454
                     /locus_tag="OO328_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5928827.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12487..13210
                     /locus_tag="OO328_000120"
                     /pseudo
     CDS             12487..13210
                     /locus_tag="OO328_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13414..14325)
                     /gene="cysM"
                     /locus_tag="OO328_000121"
     CDS             complement(13414..14325)
                     /gene="cysM"
                     /locus_tag="OO328_000121"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5928828.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14459..15556)
                     /gene="cysA"
                     /locus_tag="OO328_000122"
     CDS             complement(14459..15556)
                     /gene="cysA"
                     /locus_tag="OO328_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5928829.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15546..16421)
                     /gene="cysW"
                     /locus_tag="OO328_000123"
     CDS             complement(15546..16421)
                     /gene="cysW"
                     /locus_tag="OO328_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5928830.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16421..17254)
                     /gene="cysT"
                     /locus_tag="OO328_000124"
     CDS             complement(16421..17254)
                     /gene="cysT"
                     /locus_tag="OO328_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5928831.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17254..18270)
                     /gene="cysP"
                     /locus_tag="OO328_000125"
     CDS             complement(17254..18270)
                     /gene="cysP"
                     /locus_tag="OO328_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5928832.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18574..19365)
                     /gene="ucpA"
                     /locus_tag="OO328_000126"
     CDS             complement(18574..19365)
                     /gene="ucpA"
                     /locus_tag="OO328_000126"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5928833.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19552..20352)
                     /gene="murR"
                     /locus_tag="OO328_000127"
     CDS             complement(19552..20352)
                     /gene="murR"
                     /locus_tag="OO328_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5928834.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20516..21412
                     /gene="murQ"
                     /locus_tag="OO328_000128"
     CDS             20516..21412
                     /gene="murQ"
                     /locus_tag="OO328_000128"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5928835.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21416..22840
                     /gene="murP"
                     /locus_tag="OO328_000129"
     CDS             21416..22840
                     /gene="murP"
                     /locus_tag="OO328_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5928836.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(23020..23919)
                     /gene="yfeX"
                     /locus_tag="OO328_000130"
     CDS             complement(23020..23919)
                     /gene="yfeX"
                     /locus_tag="OO328_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5928837.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24015..24590)
                     /locus_tag="OO328_000131"
     CDS             complement(24015..24590)
                     /locus_tag="OO328_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5928838.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24651..25100)
                     /locus_tag="OO328_000132"
     CDS             complement(24651..25100)
                     /locus_tag="OO328_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5928839.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25087..25512)
                     /locus_tag="OO328_000133"
     CDS             complement(25087..25512)
                     /locus_tag="OO328_000133"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5928840.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25726..26595
                     /gene="amiA"
                     /locus_tag="OO328_000134"
     CDS             25726..26595
                     /gene="amiA"
                     /locus_tag="OO328_000134"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5928841.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26599..27498
                     /gene="hemF"
                     /locus_tag="OO328_000135"
     CDS             26599..27498
                     /gene="hemF"
                     /locus_tag="OO328_000135"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5928842.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27504..28556)
                     /gene="eutR"
                     /locus_tag="OO328_000136"
                     /pseudo
     CDS             complement(27504..28556)
                     /gene="eutR"
                     /locus_tag="OO328_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28602..29102)
                     /gene="eutK"
                     /locus_tag="OO328_000137"
     CDS             complement(28602..29102)
                     /gene="eutK"
                     /locus_tag="OO328_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5928843.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29115..29774)
                     /gene="eutL"
                     /locus_tag="OO328_000138"
     CDS             complement(29115..29774)
                     /gene="eutL"
                     /locus_tag="OO328_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5928844.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30956..31747
                     /locus_tag="OO328_000139"
     CDS             30956..31747
                     /locus_tag="OO328_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928845.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32467..32862
                     /locus_tag="OO328_000140"
     CDS             32467..32862
                     /locus_tag="OO328_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5928846.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32880..33143
                     /locus_tag="OO328_000141"
     CDS             32880..33143
                     /locus_tag="OO328_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928847.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33166..33708
                     /locus_tag="OO328_000142"
     CDS             33166..33708
                     /locus_tag="OO328_000142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928848.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33847..34134
                     /locus_tag="OO328_000143"
     CDS             33847..34134
                     /locus_tag="OO328_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928849.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34173..34379
                     /locus_tag="OO328_000144"
     CDS             34173..34379
                     /locus_tag="OO328_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928850.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34691..35176
                     /locus_tag="OO328_000145"
     CDS             34691..35176
                     /locus_tag="OO328_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5928851.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35157..35435
                     /locus_tag="OO328_000146"
     CDS             35157..35435
                     /locus_tag="OO328_000146"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928852.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35428..36888
                     /locus_tag="OO328_000147"
     CDS             35428..36888
                     /locus_tag="OO328_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5928853.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36922..37731
                     /gene="murI"
                     /locus_tag="OO328_000148"
     CDS             36922..37731
                     /gene="murI"
                     /locus_tag="OO328_000148"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5928854.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37850..38544)
                     /locus_tag="OO328_000149"
     CDS             complement(join(37850..38298,38298..38544))
                     /locus_tag="OO328_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5928855.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(39063..40436)
                     /gene="eutA"
                     /locus_tag="OO328_000150"
     CDS             complement(39063..40436)
                     /gene="eutA"
                     /locus_tag="OO328_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5928856.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40411..40890)
                     /gene="eutP"
                     /locus_tag="OO328_000151"
     CDS             complement(40411..40890)
                     /gene="eutP"
                     /locus_tag="OO328_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5928857.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40903..41238)
                     /gene="eutS"
                     /locus_tag="OO328_000152"
     CDS             complement(40903..41238)
                     /gene="eutS"
                     /locus_tag="OO328_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5928858.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(41531..43810)
                     /gene="maeB"
                     /locus_tag="OO328_000153"
     CDS             complement(41531..43810)
                     /gene="maeB"
                     /locus_tag="OO328_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5928859.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44099..45049
                     /gene="tal"
                     /locus_tag="OO328_000154"
     CDS             44099..45049
                     /gene="tal"
                     /locus_tag="OO328_000154"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5928860.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            45069..47072
                     /gene="tkt"
                     /locus_tag="OO328_000155"
     CDS             45069..47072
                     /gene="tkt"
                     /locus_tag="OO328_000155"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5928861.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(47175..48218)
                     /locus_tag="OO328_000156"
     CDS             complement(47175..48218)
                     /locus_tag="OO328_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5928862.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(48344..48919)
                     /gene="nudK"
                     /locus_tag="OO328_000157"
     CDS             complement(48344..48919)
                     /gene="nudK"
                     /locus_tag="OO328_000157"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5928863.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(48987..50966)
                     /gene="aegA"
                     /locus_tag="OO328_000158"
     CDS             complement(48987..50966)
                     /gene="aegA"
                     /locus_tag="OO328_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5928864.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            51172..52889
                     /gene="narQ"
                     /locus_tag="OO328_000159"
                     /pseudo
     CDS             51172..52889
                     /gene="narQ"
                     /locus_tag="OO328_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            53091..56204
                     /gene="acrD"
                     /locus_tag="OO328_000160"
     CDS             53091..56204
                     /gene="acrD"
                     /locus_tag="OO328_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5928865.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(56303..56362)
                     /gene="ypfM"
                     /locus_tag="OO328_000161"
     CDS             complement(56303..56362)
                     /gene="ypfM"
                     /locus_tag="OO328_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5928866.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            56743..57099
                     /locus_tag="OO328_000162"
     CDS             56743..57099
                     /locus_tag="OO328_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5928867.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            57103..58230
                     /gene="dapE"
                     /locus_tag="OO328_000163"
     CDS             57103..58230
                     /gene="dapE"
                     /locus_tag="OO328_000163"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5928868.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            58258..58458
                     /locus_tag="OO328_000164"
     CDS             58258..58458
                     /locus_tag="OO328_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5928869.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(58568..59266)
                     /gene="ypfH"
                     /locus_tag="OO328_000165"
     CDS             complement(58568..59266)
                     /gene="ypfH"
                     /locus_tag="OO328_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5928870.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(59340..61355)
                     /gene="tmcA"
                     /locus_tag="OO328_000166"
     CDS             complement(59340..61355)
                     /gene="tmcA"
                     /locus_tag="OO328_000166"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5928871.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(61370..62233)
                     /locus_tag="OO328_000167"
     CDS             complement(61370..62233)
                     /locus_tag="OO328_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5928872.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(62401..63114)
                     /gene="purC"
                     /locus_tag="OO328_000168"
     CDS             complement(62401..63114)
                     /gene="purC"
                     /locus_tag="OO328_000168"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5928873.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(63327..64361)
                     /gene="bamC"
                     /locus_tag="OO328_000169"
     CDS             complement(63327..64361)
                     /gene="bamC"
                     /locus_tag="OO328_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5928874.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(64378..65256)
                     /gene="dapA"
                     /locus_tag="OO328_000170"
     CDS             complement(64378..65256)
                     /gene="dapA"
                     /locus_tag="OO328_000170"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5928875.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            65402..65974
                     /locus_tag="OO328_000171"
     CDS             65402..65974
                     /locus_tag="OO328_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5928876.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            65974..66444
                     /gene="bcp"
                     /locus_tag="OO328_000172"
     CDS             65974..66444
                     /gene="bcp"
                     /locus_tag="OO328_000172"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5928877.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            66697..67314
                     /gene="hyfA"
                     /locus_tag="OO328_000173"
     CDS             66697..67314
                     /gene="hyfA"
                     /locus_tag="OO328_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5928878.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            67314..69331
                     /gene="hyfB"
                     /locus_tag="OO328_000174"
                     /pseudo
     CDS             67314..69331
                     /gene="hyfB"
                     /locus_tag="OO328_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            69342..70289
                     /gene="hyfC"
                     /locus_tag="OO328_000175"
     CDS             69342..70289
                     /gene="hyfC"
                     /locus_tag="OO328_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5928879.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            70306..71745
                     /gene="hyfD"
                     /locus_tag="OO328_000176"
     CDS             70306..71745
                     /gene="hyfD"
                     /locus_tag="OO328_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5928880.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            71757..72407
                     /gene="hyfE"
                     /locus_tag="OO328_000177"
     CDS             71757..72407
                     /gene="hyfE"
                     /locus_tag="OO328_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5928881.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            72412..73998
                     /gene="hyfF"
                     /locus_tag="OO328_000178"
     CDS             72412..73998
                     /gene="hyfF"
                     /locus_tag="OO328_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5928882.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            73988..75702
                     /gene="hyfG"
                     /locus_tag="OO328_000179"
                     /pseudo
     CDS             73988..75702
                     /gene="hyfG"
                     /locus_tag="OO328_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            75712..76257
                     /gene="hyfH"
                     /locus_tag="OO328_000180"
     CDS             75712..76257
                     /gene="hyfH"
                     /locus_tag="OO328_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5928883.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            76254..77012
                     /gene="hyfI"
                     /locus_tag="OO328_000181"
     CDS             76254..77012
                     /gene="hyfI"
                     /locus_tag="OO328_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5928884.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            77005..77418
                     /gene="hyfJ"
                     /locus_tag="OO328_000182"
     CDS             77005..77418
                     /gene="hyfJ"
                     /locus_tag="OO328_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5928885.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            77448..79277
                     /gene="hyfR"
                     /locus_tag="OO328_000183"
     CDS             77448..79277
                     /gene="hyfR"
                     /locus_tag="OO328_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5928886.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            79278..79460
                     /locus_tag="OO328_000184"
     CDS             79278..79460
                     /locus_tag="OO328_000184"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5928887.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            79482..80354
                     /locus_tag="OO328_000185"
                     /pseudo
     CDS             79482..80354
                     /locus_tag="OO328_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(80448..81509)
                     /locus_tag="OO328_000186"
     CDS             complement(80448..81509)
                     /locus_tag="OO328_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5928888.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            81722..83185
                     /gene="bepA"
                     /locus_tag="OO328_000187"
     CDS             81722..83185
                     /gene="bepA"
                     /locus_tag="OO328_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5928889.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            83206..83565
                     /gene="arsC"
                     /locus_tag="OO328_000188"
     CDS             83206..83565
                     /gene="arsC"
                     /locus_tag="OO328_000188"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5928890.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(83698..84444)
                     /gene="hda"
                     /locus_tag="OO328_000189"
     CDS             complement(83698..84444)
                     /gene="hda"
                     /locus_tag="OO328_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5928891.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(84494..85783)
                     /gene="uraA"
                     /locus_tag="OO328_000190"
     CDS             complement(84494..85783)
                     /gene="uraA"
                     /locus_tag="OO328_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5928892.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(85869..86495)
                     /gene="upp"
                     /locus_tag="OO328_000191"
     CDS             complement(85869..86495)
                     /gene="upp"
                     /locus_tag="OO328_000191"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5928893.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            86819..87856
                     /gene="purM"
                     /locus_tag="OO328_000192"
     CDS             86819..87856
                     /gene="purM"
                     /locus_tag="OO328_000192"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5928894.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            87856..88494
                     /gene="purN"
                     /locus_tag="OO328_000193"
     CDS             87856..88494
                     /gene="purN"
                     /locus_tag="OO328_000193"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5928895.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            88666..90732
                     /gene="ppk1"
                     /locus_tag="OO328_000194"
     CDS             88666..90732
                     /gene="ppk1"
                     /locus_tag="OO328_000194"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5928896.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            90737..92278
                     /gene="ppx"
                     /locus_tag="OO328_000195"
     CDS             90737..92278
                     /gene="ppx"
                     /locus_tag="OO328_000195"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5928897.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(92317..>93413)
                     /gene="pdeF"
                     /locus_tag="OO328_000196"
     CDS             complement(92317..>93413)
                     /gene="pdeF"
                     /locus_tag="OO328_000196"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5928898.1"
                     /translation="SHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQ
                     FRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMY
                     LQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPGS
                     FLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQ
                     LLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLK
                     NVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSL
                     GIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        22611 a        24388 c        23545 g        22869 t
ORIGIN      
        1 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
       61 aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatactcaa agattcgaac
      121 ctgcagcagg tttgagttga gcgcagcgaa acaacggagc cgctcgcggc gacggcccga
      181 agggcgagcg aagcgagtca tcctgcacga cccaccaatg caaaaaagcg ccctaaaggc
      241 gcttttttgc tatctgcgat ttgtaagatt gatttttctt ctcaagttcc ttgttcttct
      301 accgatatta cctcgtacag tgacgaggac tgaacaatgg ccactatctc ttcaacttca
      361 attccttcca tacaaacaca gagtagtaac agggcatcac aaggtagcga tgttgcttca
      421 caaatcgcgc ggataagtca gcaaatcatc aaactcactc aacaaattaa agagattgta
      481 gacactagcg gcagtgctga agacaagcaa aaacaggccg aattgattca acagcaaatc
      541 accctactgg aaacacaact ggcgcagttg cagaaacagc aggcagaaaa ggcgcaggag
      601 aaagagcagc gtttgtctct gaatgtatcc cttctaaatc ctgttgaaaa aacgactcat
      661 atcgatatct atatctgata caacggatcc ccttcccgac cagccattag cgcattgccc
      721 tggtctcgta ccacgtccca taacgcttca gcggcggtgg aaagtgaacg gttcttcctg
      781 cgcaccagca tcaactggcg ttcaaccacc ggcgtaatgc gcttaaccac cagtgggcta
      841 ccttcaggca atggtagcgc cagtgcagga agaatactga tgccgatgcc tgcggctacc
      901 atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa tattcgcctg aataccattg
      961 cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat
     1021 ttcgctccct gtaatgcctg ccagggtaca taatcttcca cggccaaagc gctatcgcga
     1081 tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac attgcagatc gcccactggg
     1141 cctggatcga tgacgatgcc aaagtcgaca tctccctggc gaatactttc catcacccac
     1201 tgctgcggac gatcatgcag gacaaactga atatctggat agcggcgatg gctttcagca
     1261 atgcattgcg gaataagatg cgcggaaatg gtctggctgg cagcgacccg aacttttccg
     1321 ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc tgttaagttc atccagcagt
     1381 cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt
     1441 ctgtccagca ggcgaacacc agtatgattt tccagctcct tcacactgtg acttactgcc
     1501 gactggctca agccgatacg ctctcctgca cgactaaaac ttttctcctg cgctactgtg
     1561 acgaaaacct ttaattgttt taaagaataa ttcatctgtt ttcttcatga atggatgcaa
     1621 taaatcaatt ttatttctca aactggaaga agcacaatag aaccatcgat catctggagt
     1681 ctttatgaaa ctttttcgta tcctcgatcc tttcacctta accctgatca cggtggtgtt
     1741 gctggcctct ttctttccgg ccagaggcga tttcgtcccc ttctttgaaa atctgaccac
     1801 cgcagctatt gccctgctgt tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat
     1861 tgctggcggt ggtcactggc gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt
     1921 tccgattctg ggtgtactgt ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta
     1981 ctccggtttt ctctacttgt gcattctccc ggctaccgtg cagtctgcaa tcgccttcac
     2041 gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct
     2101 ggggattttc ctttcaccat tgctggttgg tctggtgatg aatgttcacg gtgcaagggg
     2161 cagccttgag caggtcggta aaattatgct gcaactgctg ctgccgtttg tgttggggca
     2221 tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac
     2281 tgaccagacg tccattctgt tggtggttta tacagcgttc agcgaagccg tcgttaatgg
     2341 tatctggcat aaagttggct ggggatcatt gctgtttatc gtggtggtca gctgcgttct
     2401 tctggctatc gtgattgtag ttaacgtctt tatggcacgc cgactgagct tcaataaggc
     2461 agatgaaatt actatcgtct tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat
     2521 ggcaaacatt ctgttcccca catcggtgat cggtatgatg gtgctgcccc tgatgatttt
     2581 ccatcagatc caattgatgg tctgtgcggt gctggcgcgt cgatacaaac gccagaccga
     2641 acagttacag gcgcagcagg aaagcagcgc cgataaagct taaagcggac gcttcagggg
     2701 ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc gtgatttgca ttagctcacc
     2761 gagcttccct gccgggaact catctttgcg ggcaaaccac agcagatact cctccggcag
     2821 gtcaattaag cgacgccctt tgtatttgcc aaacggcatt atcgtattgg ctatttcaat
     2881 cagctgctct ttttccatct cagctaccca gcaaacgcag catttccgct tcgtcgatga
     2941 cttcaatgcc cagttcctgc gccttcgcca gtttagatcc tgcagcttca cccgctatca
     3001 ccagatcggt tttcttcgac acgctgcccg cgactttcgc gcccagttcg accagtcgag
     3061 ctttagcgtc atcacgcgac atctggctta agctgcccgt aagcaccacg gttttaccag
     3121 caaacgggct gtcaatctct tccgcgttga taacgatcgg cgcaggccag tgaacacctt
     3181 ccgccaacag ctcgctgatg acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt
     3241 gggatgcaac gacaatgcca acatcaggca ccttttgcag ctcttcaatc gaagcggctt
     3301 ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc tgcggtggcc tcgccgactt
     3361 cacggatgcc aagtgcatag aggaagcgag caaaggtggt ttctttcgct ttttccagcg
     3421 cgttaaccac gttttgtgcc aattttggcc ccatacgctc cagtccggtc agtttgcctg
     3481 cggtgagttt gaacagatct gccggagtat ggacatattc tttttcaacc agctgatcga
     3541 tgattttgtc gcccattccg tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg
     3601 actctttacg ctgcgcaccg caaatcaggc caccggtaca gcgggcaacc gcttcacctt
     3661 ccacacgctc aacgtcagaa ccacataccg gacaatgcgt cgggaataca acctcacggg
     3721 tatcttccgg gcgttcagaa agcacgacgt taaccacctg cgggatcacg tcgccagcgc
     3781 ggcgaatcac cactttatcg ccaatgcgta aaccaagacg ttcgatttca tccgcattgt
     3841 gtaaggttgc gttactcacc agcacgccag caacatggac aggttccaga cgcgcaacag
     3901 gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc acgcacaaag gtcatctgct
     3961 cctgcgccgg gaatttaaac gcaaccgccc agcgcggggc acgcgcgaca aagccaagct
     4021 gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg
     4081 tcgggcggtc ttcttccact ttgtgataga acgccagcac ttcttccgcc gattcacaaa
     4141 gcgttacccg atcgctgacc ggcacccccc actttttaaa ttgcagtaaa cggccaagat
     4201 gagtatccgg cagctcgcca ccttccagaa caccaacgcc atagcagaaa aaagtgagcg
     4261 gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga accagctgcc gcattacgtg
     4321 ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg
     4381 cctgcggcag gaacacttca ccacgcactt ccagacgcgc cgggatattc tctccgtgca
     4441 gcttcagcgg aatggcgcga atagtacgca cattagacgt gatatcttcc ccggtggtgc
     4501 catcgccacg ggtcgcggca ctgactaaaa cgccattttc atacagaata ctgacggcaa
     4561 ggccatccag cttcagctca cagcaccagg tgactttctc gttgtttttc agacggtcct
     4621 gcacacgttt gttgaaagca agaaagcttt cttcatcaaa aacgttatcc agtgacagca
     4681 ttggtacttc atggcgtatc tggctgaaag ctgccagcgg cgcagcacct acacgttggg
     4741 taggcgaatc aggcgtaatc agttctggat gtttggtttc cagctcgcgc agttcgcgca
     4801 tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc catcacatga taaagatatt
     4861 catgatggca aagcgtcgtt cgcagttctg tcagttgttg ttcgattgat tccatatcgc
     4921 accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa
     4981 gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt cctggtactc gcgcaatttc
     5041 tgcggagtca tcatacggcg ctgatcgtca agcacgacac cgcccacttc atcggcaata
     5101 tgctgcgcag attgcagcat cagcttgaag ttctgcagct cgtcaccgta agacggtacc
     5161 tgcataaaga tagtgacacc cggagtagtg aaatccttca tttcaggatc aaaggttccc
     5221 ggtttcacca tattcgccag gctgaataac gccgggccgc tgccatccgg gctaagatga
     5281 cgatggtaaa tattcatatc gccaaaaatg aagcccgctt gttgaatgct gttaagaagc
     5341 agttcaccgt ttagctcgct accgtgatgc gccgcgacgt tcatgataat caccgcttct
     5401 ttgcgcttcg gtttatccat aactggagcc ggttccgcta caggctcagg ctgtggtgcc
     5461 gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc
     5521 tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca acggctgttc aggctgcggc
     5581 tgataggcag gctgctgcac cggctgcgct ggacgcggag catgttgcgg cggtacctgc
     5641 gcttcaggcg gctgctggac cggttgacgc ggctgcgcag acgcataagg cggtaggtac
     5701 tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc
     5761 acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa
     5821 tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt
     5881 tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca
     5941 atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc
     6001 caccacggca aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac
     6061 tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat
     6121 atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt
     6181 caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt
     6241 tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt
     6301 ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag
     6361 ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt
     6421 tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc
     6481 acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca
     6541 cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg
     6601 cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta
     6661 ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg
     6721 tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg
     6781 ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt
     6841 gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc
     6901 gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg
     6961 taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc
     7021 ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac
     7081 ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg
     7141 acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt
     7201 tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag
     7261 cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt
     7321 gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc
     7381 ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat
     7441 tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa
     7501 aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata
     7561 cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg
     7621 tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac
     7681 tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct
     7741 tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga
     7801 agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa
     7861 cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac
     7921 cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt
     7981 tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat
     8041 tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga
     8101 gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg
     8161 tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga
     8221 actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc
     8281 aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata atgtttaatg
     8341 acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc
     8401 gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga
     8461 ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt
     8521 accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct
     8581 aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc
     8641 ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa
     8701 ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa
     8761 tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt
     8821 tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca
     8881 ttgaccggaa aaaaatttct gccgaccagg ttgatcagga agttgaacgt tttctgagcg
     8941 gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg
     9001 gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg
     9061 agcaggaaat catagccctg attaaagata aacacatgac agctgacgca gctgctcatg
     9121 aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac
     9181 gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga
     9241 ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt
     9301 ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc
     9361 gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg
     9421 gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc
     9481 aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag
     9541 tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg
     9601 gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc
     9661 gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg
     9721 cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct
     9781 cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga
     9841 acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc
     9901 gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat
     9961 tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga
    10021 tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa
    10081 tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag
    10141 ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg
    10201 gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca
    10261 agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg
    10321 ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga
    10381 gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga
    10441 aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta
    10501 acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct
    10561 taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag
    10621 gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac
    10681 gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg
    10741 ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac
    10801 gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc
    10861 gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt
    10921 ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg
    10981 actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc
    11041 gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa
    11101 atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg
    11161 ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc
    11221 gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg
    11281 ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca
    11341 ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc
    11401 acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg
    11461 cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc
    11521 agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta
    11581 tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc
    11641 gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt
    11701 acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac
    11761 cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat
    11821 accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg
    11881 ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc
    11941 ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat
    12001 cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa
    12061 acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac
    12121 actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga
    12181 gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga
    12241 tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc
    12301 cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt
    12361 gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca
    12421 gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga
    12481 ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata
    12541 gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc
    12601 catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc
    12661 gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat
    12721 tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat
    12781 tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa
    12841 ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc
    12901 tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga
    12961 agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat
    13021 tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa
    13081 aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg
    13141 tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactg cttttccaca
    13201 cgaaaattaa aaactgcatt tgtcggcagc aacaactgcc gacaaatcag attattgaca
    13261 aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc
    13321 taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt
    13381 gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg
    13441 ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac
    13501 caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga
    13561 gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc
    13621 gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata
    13681 ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac
    13741 aatggtcacc ggtttggatt gttcgcgcat aaagcatgaa acgccggtga tagtgccggt
    13801 cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    13861 cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt
    13921 tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc
    13981 tttggtgaca agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct
    14041 catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag
    14101 cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc
    14161 gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg
    14221 gttattgcct tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa
    14281 cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacaatct ctatccatac
    14341 gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc
    14401 cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc
    14461 aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat
    14521 acagccgcgc atgttgcaga ccaacgaata aacgctcgcc atgctgcggg gcatcgtcgc
    14581 catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg
    14641 tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg
    14701 tacggcggct gatatccact tcccacgggc gcaggaagag atccaccggc ccctgatacg
    14761 caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg
    14821 ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc
    14881 gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac
    14941 gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca
    15001 gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg
    15061 cgccgaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg
    15121 ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa
    15181 gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct
    15241 cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc atatggcgga
    15301 acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca
    15361 cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc
    15421 gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag
    15481 gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttgatattgg
    15541 caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc
    15601 cactgcaaca tactctttaa aaacagggtg ataatcgcca tcaacgtcaa cagcgcggcg
    15661 gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg taacggcagc
    15721 gaaagtgttt cgccgcgaat cgagccggaa accaccgaca ctgcaccaaa ctcgccaatc
    15781 gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg
    15841 acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg
    15901 ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag
    15961 atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc
    16021 cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc
    16081 ggcgatacag caaacggaat gtccagtagc gtcagcagta actggcgtcc cgggaagtta
    16141 aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc
    16201 agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt
    16261 aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc
    16321 aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc
    16381 gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc
    16441 gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga
    16501 tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca
    16561 tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa
    16621 attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct
    16681 ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt
    16741 cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa
    16801 tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact ttgatatcaa
    16861 acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac
    16921 cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
    16981 ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa
    17041 tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg
    17101 tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcagaga
    17161 gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt aaagtaaagc
    17221 ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt
    17281 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg agccaaattt
    17341 gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc
    17401 cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag
    17461 ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac
    17521 ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg
    17581 tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc
    17641 caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
    17701 gtttttcagg aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt
    17761 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
    17821 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg
    17881 gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
    17941 gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt
    18001 cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt
    18061 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
    18121 ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
    18181 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
    18241 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
    18301 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
    18361 atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt tgcaggcagt
    18421 ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg atttcgagca
    18481 ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc
    18541 tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa ccgtctccgg
    18601 cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg attcatccga
    18661 tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac acagcgggat
    18721 tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact ggcgggcaat
    18781 gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc gaataccgga
    18841 ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt tcgttaaggc
    18901 atacgccgtt tcgccaggat cggccaccat atcaccagtg actgaagaca tcatcacaat
    18961 gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc
    19021 tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc
    19081 cagacgacaa acgcctgcgt tattcaccag gatatcaatg cgcccttctt tttccttcgc
    19141 gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca
    19201 gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg agatatccag
    19261 caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc caattccctg
    19321 caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa tgctctcctt
    19381 gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca aaatattgct
    19441 gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat gcatcatttc
    19501 aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac atcattgatg
    19561 ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc gggtagacat
    19621 cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat gcgccagccg
    19681 ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct gctttcgtgc
    19741 cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag cgatttgtac
    19801 atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc
    19861 cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca gcgccgatcc
    19921 gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga ttttttgcag
    19981 ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt cgcgattcag
    20041 tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc
    20101 cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca tacgcaattc
    20161 agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact ggctgatacc
    20221 gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc tggcccgcag
    20281 aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc gtattttcgt
    20341 caggtacaac atcttccact ctcctgggct ggcagcaaaa attccgctta agttcttatg
    20401 acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt caattcggga
    20461 tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac caccgatgca
    20521 actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg accgcgtatc
    20581 gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc ctgccgttga
    20641 tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg
    20701 cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    20761 cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga ttgctggagg
    20821 cgaatatgcc attcagcacg cggtggaagg agctgaagat agccgggaag gtggtgttaa
    20881 cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg ctgccagcgg
    20941 tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct gccgcacagt
    21001 gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg ccattacacc
    21061 gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggta cagcgcagaa
    21121 actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    21181 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    21241 taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg cctgcgagcg
    21301 caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa
    21361 acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat aacccatggc
    21421 caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa
    21481 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcact
    21541 ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgacca
    21601 ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct
    21661 gctgggcgat gcaccggtac aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt
    21721 aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    21781 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    21841 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc tgaattttat
    21901 gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct ataacgccgc
    21961 ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg
    22021 ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct ttggtctgcc
    22081 catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgtgggcct gcgcccgcat
    22141 tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga cctcgttaat
    22201 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gtggctggtt
    22261 attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg gttgtgcggt
    22321 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    22381 ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    22441 aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac cgcacagtgc
    22501 gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg gtgaaccgct
    22561 gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc
    22621 ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg gcttaaacag
    22681 cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac aaggcatcct
    22741 tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg ggtttatttt
    22801 caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa cggacaatgc
    22861 tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac cctgttctga
    22921 cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg ccagtcagta
    22981 atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc
    23041 cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc
    23101 acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg
    23161 cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct
    23221 ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga
    23281 ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat
    23341 catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg
    23401 cacaaacaca tagctgccgc cggaatccac gccgtctttg ataaccgcca cttcgcggcg
    23461 cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc
    23521 cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat
    23581 cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac
    23641 atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc
    23701 tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc
    23761 aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact
    23821 ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg
    23881 gcaatgttct ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataagaa
    23941 tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc gacggcggtt
    24001 tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt tttcagggta
    24061 tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg gtagctaata
    24121 tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg
    24181 caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg
    24241 tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta
    24301 atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac gttgccgttc
    24361 gtggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc aatggcttgt
    24421 tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac tttggtggac
    24481 gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag
    24541 ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat gtcacttcct
    24601 ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt
    24661 tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata
    24721 tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac
    24781 ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag cacatgccag
    24841 gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg
    24901 ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta
    24961 ccctgctcac gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat
    25021 agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga
    25081 tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg
    25141 ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat
    25201 ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt
    25261 ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg
    25321 cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa
    25381 caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg
    25441 cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac
    25501 gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac
    25561 ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt
    25621 caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa cggattacgc
    25681 gatacgatat aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa
    25741 ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc tgccctgacg
    25801 ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag caacggacac
    25861 agcaagccga aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac
    25921 ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg
    25981 gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg
    26041 cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc
    26101 gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct
    26161 tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa
    26221 cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg
    26281 cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgcttggc
    26341 tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa
    26401 gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt
    26461 atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc
    26521 acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat
    26581 tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac
    26641 cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat
    26701 agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt
    26761 ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc tgcttccgcc
    26821 accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta
    26881 gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc
    26941 gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt aacccctttc
    27001 tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgccagcca
    27061 tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa
    27121 catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa cacgccagat
    27181 ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta
    27241 ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc
    27301 taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac gctgtttggc
    27361 ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg
    27421 gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag
    27481 gtcagggatt gggtgtaact ccctcacccc cattcccgca tccgctgatg caacgtcaac
    27541 gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat
    27601 ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg
    27661 cggcgtacga cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata
    27721 gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca caaatccagc
    27781 accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga
    27841 cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc ttccagcatc
    27901 gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga
    27961 ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct
    28021 ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc
    28081 ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac tacgcccagg
    28141 atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg cgtcgctatt
    28201 tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg
    28261 ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat
    28321 tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc
    28381 ggatgcagct gatcgtaaat ctgctgctag gcggtaatcg tcaaagcatg ttcatagaca
    28441 tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt
    28501 tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg
    28561 ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga
    28621 taacgactac tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag cgcatttctg
    28681 gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat tccctgacat
    28741 actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg
    28801 ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca
    28861 tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca
    28921 caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag
    28981 cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca
    29041 tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc
    29101 atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg
    29161 gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag
    29221 tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc agatttcaac
    29281 gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg
    29341 ccaagcgtga tcccggcggt tgatgaaaga taagaaccgg tacgcgaaac cacatgcgcc
    29401 aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt ttcaatatgt
    29461 gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc
    29521 agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag cgagcgacca
    29581 tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg
    29641 tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc
    29701 gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa
    29761 tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc
    29821 agcatacgtt tcgccaaatc cacaatgacg gcacagcagc acgaaagaga agcaaaagtc
    29881 agctacctgg tacactaggc agcgggtcag caaagggggg caaatgcccc ttttttcatg
    29941 tcagataatc agatcattac ctcaataaac taataatcac atcgcggtta caaccagttt
    30001 gatagtgata gctcaaaccc agacgtggcg ctggctgcaa gcaaaataag ccgcaaaaat
    30061 gcgaccccat gtttactagc cgcaaaaatg cggtcctatt tgccagaacc ttttattatt
    30121 caattggtta gtgcaagaaa aagcgtgatt actgttcaag aagtatccag aaaggtcatg
    30181 attttgacgc tgaaaaaaaa taggtgggca tttttgccca ctcactagcc gcaaatttgc
    30241 ccactcacta gccgcatttt tgcggcccaa agtaataaat acagagaaat aataaataca
    30301 gatcagcgcc aagatttttc gtcttttcgg cggaatcatg agactagtgc ttacgtcctg
    30361 ataacctcca gagcagacct taacttctgt gcagcaggac tacgctcatt gccgctcggc
    30421 taggtcaaat cctcacgata aagctgttcg gattgtgacc ataatgttaa aggactcttt
    30481 aaatcattcc ttgacgaatt aactagctgg cttaagcgga atagcatgca ggataaaaac
    30541 aaaaccagct tagcagtggg gagagcttcg cttcgctcgt ctcctggggc caatgctcaa
    30601 tgaaacgacg taaggcacgg agcgctcacc ataggtagac acataaacat ctgagagcgc
    30661 accttctatc gcgctacgcg ctcaataaaa accaacgaca ccgccacact gcaagaccaa
    30721 tggtttttat cgcgtataca gaccagacag gaaacagcag caaagcagcg tatagccagg
    30781 tgcagacagc acactcacac gacgcacagc aggcttaagt gatcactcag gaaactttat
    30841 ccccgccgcc gatcaccgcg aactatcatg accgcgccaa attcgaaagg aatggcccat
    30901 agaacgcgat gaacacacat aagcgctaca cttacttaac gtcgccacag agcgattgag
    30961 agcgcttaaa atcgatgcga tttcattaaa tttttctcac tcgcctgaat ggtggcgatc
    31021 aggactgatc gcaacattgc cccgcctcga tattgagcgc ataatagata catacattaa
    31081 gcggaaaaga gataagttta ttgtgaaaat cagggggtta catatcatgc gcatatcaga
    31141 acaacaacgg cagatcctcg actatctggc aaaaaacgaa tcaatcaccg cacgacaagc
    31201 cgcagatctc gtttatggtg gcaatgttac ccgcgtccag gttgacgccg cccgccgctc
    31261 actcatgacg atggttagta atgggctaat caggaaacaa ggccgcgctt tcgtagctga
    31321 aagacatatc aatcacgctt atagccagga cgttgaagaa ttgcgccgcc agatcctttg
    31381 ggtattcagc aaacagggtc aggagtattt cctttctgaa cacccaaatt tcgccgccaa
    31441 agagttaaca gtaagcggat tgtcgtgcgc actggattat cttgtcggtg aaggttgcgt
    31501 tacttacaag cagctatggc acgccctgaa tacgcttgta gaagatggtt tactcattgc
    31561 cacgcgtgag aacatcgaat actggataat gccgcgttca ggtcgccgca atcatgcacg
    31621 gtatatttca ggcaacagat acagaacgcc ggactttaat ccgacacagg aagatcggga
    31681 ccgtgccgaa atgatgtgca gggaacagga gaaaagaatt atggataagt tattttcgat
    31741 gcagtgaaca caagcgggcg caatgcccgt ttttattgcc ttattccacg cctgacgaat
    31801 caatttgatc acacctgaga aaatgcatag ccagaagtta aggccgttat attgcgattg
    31861 ggagcgtttt aaacggtatc aatttaatta attctgatgt ggtgatttgc aggcaaaaaa
    31921 aacccgccgg agaagcggga aatagagaca tatcgttgta gttattaaca gatcactttg
    31981 aggtcgaggg cggctaaccc tcgctaatat tatcccgcta ttaccttgcg ggtgcaaata
    32041 atcttttatt gctttgatga tttaataaat agcacttttt gctaacaaaa accgctgtca
    32101 atatatggcc aaaaatgtca acagaaaaaa taaggtttat aatattaagt atcacatggg
    32161 taatgggttc agggaatcgg aatccaccgc ctcacggaaa ttcaaaattt gcttcttctt
    32221 tcctccgtgg gcggcttctt tttcgtatcg aggtatttat gaaagcaata acattagaac
    32281 agttcgcagc aatggcgaat gtatcgacca gaacgctgag gcgctggcta aaaaaaccat
    32341 caaccggagt ccctttgcca tttaccccac gcggtactcg ccaattattc tgggagcacg
    32401 aatgcaaaga atggttgtac agaacagaac gataataatc acgcatcaca ttttgaggta
    32461 aacatcatga ccaatatcga aagattagaa cgctgtgccg atattatgcg acgcagatgg
    32521 atttatgacc ctgaagcggg tttattgttt agccgtgaaa caaaagacgt tatcaaaggc
    32581 agcatgacca ataaagggca tttggttgta acggtacatg aaggtaaatt caaagtgaca
    32641 ctcacctatc agaaagcctg ctatgtatac acatacggcg cttatgacga gacattatat
    32701 gaagtgcacc acgttaattt gaacaaacag gataaccgca tcaagaatat tcgcctgatg
    32761 ccgaaagaaa agcaccgccg gatacataaa aacgtccgca aacttattcg gctgggccac
    32821 acacaatggc ttcaaccgca attaattgaa caatcactat aaacacagga gtaaatacca
    32881 tgcattcatt agacagttat tttcagcgca cgacagcccc taaatcagcc gcccaggaac
    32941 gccgtgagga attccatgag aaagtgatgc gttctgctga ttatattgcg gacaagttcg
    33001 tggaaacggt tcgtccactt gttgatgaag tagctgataa acttcagtct gaaatgccgg
    33061 aagatatgga aggcactgca aagcggcgac tcatttgcga attaagtcgt cgttttggcg
    33121 tgtcaataag cgcttttaaa taatcgaaaa aaggggggaa agataatgga cgcatttgag
    33181 gtactggcaa ataaaggcgc tgaactggta gcacagcgtg ataaaaccgc caacgaaggg
    33241 gaacgcgcac tgctaaataa acagattaaa gcgatccgta tggcccagtt taaattaatc
    33301 agtaatgaag ttatcgaaac aattaaaccg cagatcccac aaatcgttgc tgacgctatt
    33361 aaagccgccg gactggagaa acgcattgcc gggctaaaaa cgaacgcaga gaaaatggag
    33421 atgtacaaac agagcggtgc tccgaatccc gatgagtact tctcaccgga tgttgagttt
    33481 atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg tcgctatttt
    33541 gatggagtgg atagttcggc aggtattgac ctgaactcgt atttcggtcg tgaaattgag
    33601 cattttgatg ccgccccgtc tatgccggag ccagaaccag aaatggccac cgatcctgaa
    33661 gctgagcaac ggaaacgcgt attcaatgcg atagggattc ggtactgatc ggataaaaga
    33721 aagccgggaa ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc ggacaagaca
    33781 aacaggataa gcagtctcga aacaatgttc gggaactata ttagcacaag tccggagaaa
    33841 atcatcatgt tggcactacg catcatgcag ggcatcgcta agaccctcgc agaacatgtc
    33901 ttagacctga aacattcgcc actgagcaaa caggccatga agcgccaaac gcttcgtctg
    33961 tgggctgaat attcgcttgg aactatcaat aaaatcatcg acatgaaatc ggggccgagc
    34021 aaccagagcg cggaggaaat ggagttcatc aggcgtttaa ttctcattcg cagagacatt
    34081 catagccagt tacacagcgt gggaatcgat attaatgatg ggacgggcga ttaattttcg
    34141 gacaagtact atgcgcgcgc gaggagctga acatgaagca agatattgca gacaggctgg
    34201 agatactcga aggccagcgg gcagaagcga aacaactacg taagcaggcc agaagagcgc
    34261 acaggaacaa cgaagcagag ttactaacca aatacatcag tttcaccaat tactgcatct
    34321 acgagtgtta caaggaagat gcagaggact ggctggattc attgccagaa cagtactgag
    34381 cagaataaca ccacgtcatg ttgttctgag gtgagcatat cacctctgtt caggtggcca
    34441 aatttagtgt gccacttcag catcaccgtt gaaatcacaa taacgatttt tttgtaaata
    34501 tgctctcaca tatgtaggaa tagaccaggt tggcattgaa acacatctac catgaccata
    34561 ataaagtaat tttttgatat gtttatggtt ttatattaat taaaatatat ctaaatttga
    34621 tagttttaaa attgcaaaat ccagattgtg tttgtaatgt ttaatctgat tgtattgagt
    34681 ggcgtttatt atgaaaaata aatcaggtaa ttcttgttat agagaagcag tcgatattca
    34741 tagctcactt gagctttcat ttatattgca taaatatcat ttgcgtgtag atgagaagaa
    34801 atctcgaaat gtattcagcg ccatacgtga tgagctcatc cttgatgggg attcacgaca
    34861 aaacttagct actttctgtc aaacatggct tgatgatgaa atccgtgagt tgcttaattt
    34921 agctattgat aaaaacatga tttataaaga tgaatatcca caaatggcgg aacctgaagg
    34981 tcgctgtgtg cgtatgcttt ccgattcgtg gagtttcccc gattcgcgac acaccttagg
    35041 ttgctcaacc attggttcat ctgaagctgc tatgctcggc cgcttggcgt taaaatggca
    35101 gtggtgtaaa aaacgcgaag tacaaggtaa atcaaccgaa cctgatttgc gatcctgtgc
    35161 aaatatgctg gcataaattc tgccgttact gtgatgtgaa attgtgtgaa attccacttg
    35221 atgatcatca cctgataatg aatgctaaag aggttttgaa gcatgtcgat gaaaacacta
    35281 ttggtgtcgt tacgacttta ggtattactt atttttgtaa agcagattgc gttagtgttt
    35341 ttaagataag tgttaaagtt aatattagtt tttatattta catcgtaatt atatgtttct
    35401 attttaaaaa tgttcgagag tataaaaatg tctaatgcta atatcgcacc caaaagtaag
    35461 aaaatgacag tggggacact cgctatcatg aatataactg ctgtagtaag tctacgattt
    35521 ataccttctg aagcagaata cggcttaggt gccatttttt actatacctt tgctgcaatt
    35581 atgtttttag tacctgttgc ctttgttgcg gctgagctag ctacaacata tccacaaaaa
    35641 gggggggcat ttcgatggat aggagaagca tttggggctc gctggggttt tgtaggaatg
    35701 cttatgacct ggctacaagt catcccctac tttccaacag tactgacatt tggtgcagtc
    35761 tcggttgcat ttattgatgt tgatatgggg gtggcagaat caattgccag taataaatgg
    35821 tatattttat ttttcgtact atttgtttac tggggagcta tttgtgttgc attacgtggc
    35881 gttggtattt tctcgcaggt atctaagtgg tgcggtatta tagggacaat tattccagct
    35941 attatccttg ttattcttgg tttctcctat ctgtggttca gcggtaaacc agcggtgatc
    36001 ccattaggtt ggggtgatct tttccctaat atagctaact tccagaatat tgttcttgcc
    36061 gccagcatat ttcttgctta tgcagggatg gaaatgaatg cggtccatgt taacgattta
    36121 gataatccaa ctaaaaaata cccaattgca attaccatcg cttctatagg tactattgca
    36181 atattcctgc tcagtacttt aggggtcgcc tttattattc ctacagataa aattagtcta
    36241 actcaaagtt tgttggttgc ctacgatatg ctgttcgaat gggctggagt gccatggctt
    36301 ggctctgtga tggcatttat gctagcaatt ggcgtactgg gtggggtggt tacttggatt
    36361 gcagggccta atacaggtgt attagcgatc gcgaaagctg gttatttacc taagtttttc
    36421 caaaaaacca atcgtcatgg aatggggcac catctaatgt ttgtacaagg tataattgtc
    36481 tcagttcttt ctgttacttt tgtgataatg ccttcagttc aagcagcttt tcagatttta
    36541 tcacagttaa cagttatgct ctatttagtt atgtatatgc tcatgttcgc tagtgctatc
    36601 tatctacgac attctcaacc tgatacaaaa cgcccatatc gagcccctgc tttatttttc
    36661 tgggcaacac ttggattctt agggtctttt ctggcttttg tattaagttt tattccgccc
    36721 gagcaaatcc caactggtag tccaaccagc tatgttcttt atttgttagt attagttgcc
    36781 ctatttttat taattccaat attaatttac gccttccgaa aatctgactg gaaagatcca
    36841 aactctgatt tcgaaccatt ttcttgggaa aaaagaacta gtaattaaat tttccataag
    36901 ctacaaaaga tgaggttaaa tatgtttagt gctgaatctc caattggtgt aatagactca
    36961 ggtgtcggtg gattgacatc ggtaaaggaa ataattaatt tgcttcctgg tgaagatatt
    37021 atctattgtg gagataatat gaatgcacct tatggcaacc gttctgccga tgatatcatc
    37081 agtttgacta aaaaaatgct gactttttta cagtcaagga atgtcaaact agttgctgtc
    37141 gcatgtaata ctatatcctc aactttggaa tccgaagaat acagcggata tgctaagagc
    37201 tttccgtttc ctatcttatc catcattgaa cctgcagtag aagatgtgat tcgccagcaa
    37261 tataaaaatg ttggtattat cgctaccgag tttactatta aaacggggtg tcacaaagag
    37321 ctaataaaaa aattgaattc aacaataaat gtatttggag aaccaagcaa aaatcttgca
    37381 atgttaattg aggaaggtaa tcttaatgct cctgcaatct tgaatgacat caaaaaacat
    37441 gttaaccatt taatttccct ccatccagta aatgaaatta ttctcggctg tactcactat
    37501 ccaattgttc agaatttttt tgaagcagtt gcaccggata taaaatttat taatcctgca
    37561 catgatcagg ctatctctat taaaaatcac ttgggacagc taaatttatt aaactcctca
    37621 aatataggaa ctttgcaaat aaatactagt ggaagtatgg agatctacaa aacggtacta
    37681 ggggaattga gcattacgaa acctcataca ttcagtatca ggcagtttta aatttatccg
    37741 gtacaccgta aaatcccgtc cttctgaact gcaccccaaa agttggacac caactgagta
    37801 aggtgcattt tatggacaaa cccagttaca tactgggtag tgcatccaat tagtagaaca
    37861 tgtgtttttc gataaacgct ccgatcactt tttcgtggat ctcaactgag cgtgagaagc
    37921 agattgtttt acgacccaac cgcttaatgc gggtgcgtag cgtcagatta ttacgctcaa
    37981 tgcgttgggt gaatattttg ccggtcagat gcttattctt cggcacctcc cggccatagc
    38041 tgccccagtc atcgctggtc agcatgccga tgttgaaggg tgtaagcagt gccagtagct
    38101 cccggcacgt ttgatcggtt cggggaccaa aagtgtaggc cagtacaccg cctgttttgg
    38161 tgttgtacgc gtacccgagc caatgttgcc gggctttact gccaacgtag ctccattgct
    38221 catcaagctc gcagataaac gccacatcag catgggcaac aggcgaagaa gttattcgct
    38281 ttggcgcgag ttttttaaag tccggatgac ggtgttacta ccaattttca gtgtcctggc
    38341 ggtatcgcga accccggcac cattaaaggc catttcagtt atcagctctt tcatacccgg
    38401 cttacgtgct tgataagtat aagtgaactg aaacacacgg tggcagtcac ggcagcgaag
    38461 tctgtcacgg cctttagggt tctgaccatg gcggtaaacc tgagctgact gacaacgggg
    38521 acaatgaata ttaacgctgg tcatgagata acctcaaaag cccgtattat acatcagatt
    38581 caactaatta gaggcatcac ccatccagca ttataaaaca tactaacaat aactaaatta
    38641 taataatcac aacttacaat caaataataa atgcataaga ttaacaatga ttttttcggg
    38701 ttatatttat ctaaaaaagc agaacattat gtaacttatt attttctaag cgaaaattta
    38761 ttgtgcggtc agactaacaa acttgcgtac cacccgaaga acatgcgaag atttaagtat
    38821 tctttctttt ttggttttta tttttcattt tctcttttct tctctttccg gttctgttct
    38881 ggttttggtt ttttcctttt tatttttatt ctttcttcag ggttatgcta aaacaccaaa
    38941 acatttttca ccacaaaagc atccaatcat cttgccagac caatcacctt aaccggatac
    39001 actgccggat ataatcccac taacacccag acaccaagca ccatacctga taactaccgt
    39061 attcaggaag gaaatgcgag tgatttcacc gtcaccggca caaccgatcc gccaaaaaga
    39121 ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca cttcgtcaat gactgccaac
    39181 gggagttgtt gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa gtcctgcccg
    39241 gccaccacca gcaggggatg cggattcgga aaacgcgcga cgaaatcgac cagcgcgttg
    39301 atgaccgtca gaaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc cagcacgtac
    39361 acgtcagttt tgggatcaag atccagctga agcagcgcct gttgccaggc actcaccaga
    39421 tccgtttcat caatcgggat cgccaccggc aaattgcgca gcggcagttg tacgccctcc
    39481 agccagattg tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat caccgtggag
    39541 cgtacggttt gcgccggaaa ctgcacattc atctcacgca ggcgcggatg gtcatgcagc
    39601 gccgttgcca gaagtgggcc aatatcggca aaacagaacg ggttggcggg ctggtggcga
    39661 taacattcgc ccacgcctcc agaaagcgta atgatttcgg gcgtaacacc tgcgggcagc
    39721 aaaccggttt gcatcaatgc ctgcgcgagc ggcgaaagcg ttccgtcaat cacttcgaca
    39781 atcagcgccg ccatccgccg ggtaacctgc accagctgcg cgccggtcag cgaacgggcg
    39841 tcagtgcctg caccgaagca ctcatccaca atcatctgcc ccggtttatg agcgtaaacc
    39901 acgcgcccct ggctgtcggt ttccagcagg cgaccaccga cgttgagaca ggcagtgccg
    39961 ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat atcgatattc
    40021 agtacccgac acagccgttg ttcagaaagg gtttgcgccc cggctccgtg accggcgatc
    40081 acggattcga ggtgcggccc ggcgctggcg acgacaaaat cgcccagcga ctgtttttcc
    40141 ttcatcactt tttccatcag ctgtgcgacc aggctttcgg taaactggcc ttccggcagc
    40201 tgggcaatga tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg cacgctggcg
    40261 ggcacggacg ccgttaccgg gatcgattca tcacattcgg tgatggtaaa acccagtaga
    40321 tccgccactt cacgggcttc cggggtaata atgctggcgc gcagaaccac ggacattgcc
    40381 tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt tttcacctgc
    40441 ttcctcctgt ttggtgagag atgccagata atccaccagt tgctgcacgc tttgcgggtc
    40501 gtggctgttt agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata actttcgcgt
    40561 tgcggcgacg tcagcatctg gcatgtccgt tttactgatg acggcgattt gccgtttgct
    40621 gacgccaata tccagcaacc cggcaggcaa ccgactttcc gggtcatttg cgccatggac
    40681 atagatcagc atgtctacat cctgtagcgt ggtaattaag gcgtgatacc agcggggatg
    40741 gctaaaatat tcacccggcg tatcaatatc gcccttatca ttaaattcca cggccagtgt
    40801 ttttctggcg agggtataat tcccctgcaa cgcattaaat aacgttgttt ttcctgcccc
    40861 gacgctaccc acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt tggtcatttc
    40921 acaaagcgta taatttaata agcgacctaa accgctgacg gtttgtgata acgcttcttc
    40981 tactgcgccc accgagccat aaatcaccaa tgcaccgctg aagcggtcga gaaaaccgat
    41041 atgcacatca gcggctttca gcgctaaatc accggcgatc atcgccgttt cgccgggagt
    41101 tagcgtcata atgccaattg cgcccgcatc cggaacgccg attttcttcg ccagttcctc
    41161 accgggatgc gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa attcctgaat
    41221 gatgcgttct ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc gtgacaattt
    41281 ttcgtcagag taaataacaa aattcggcaa gcgcgtttaa aaggtgaggt agatcaataa
    41341 gaatggagag atatttactg ttatgaataa actttcatat gcgacgcatg aatataaata
    41401 aaataatatc gagatatttt atatactgat ttttttatta ttcgcaaact cagtaataag
    41461 ctttgctaat atatttcagg aaaaccgctt accctgaata ttcagggtaa gcgtgagagt
    41521 taagaatgaa ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc accatattga
    41581 cgatacgacg caccgatgcg atcggcgtta acacgtgaac cggtttcgcc acgcccatca
    41641 gcaccggacc gacagtcaca ccttccgagc tggaaacgcg cagtaagttg taactaatgc
    41701 gggcagcttc catgttcggc atcaccagaa tattggcaga acctttcaaa gggctgtccg
    41761 gcatacggtc gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt tcaccatcaa
    41821 tcatcagatc tggcgcacga tccctgacca gttccagcgc ctgacgcatt ttgctcgacg
    41881 acgggcagtc agaagaacca aagttggagt gcgacaacag agcaacgcgc ggctcaatac
    41941 caaaacgacg gacagtttct gccgccatca aggtgatctc cgccagctct tctgcgtctg
    42001 gttcgtcatt gacgtaggta tcggcaataa aggtgttacc actcggcagc agcagcgcat
    42061 tcattgcacc tgcggtgtga acgccatcgc gataaccaaa gacatttttc accacactaa
    42121 aatgttcatg ataatcgccc accgtaccgc agatcattgc atcggcttcc ccacgctgaa
    42181 ccatgatcgc gccgatcact gtcgggttac tgatcagcgc acgctgcgcc tgttcctgag
    42241 tgacaccgcg acgcttcatg atctggaagt attcggtcca gtactcttta aagcgcggat
    42301 cggattcgtt attgacgatt tcaaaatcaa cgcccgcttt gatctgcaag cccagtttct
    42361 gaatgcgcat ttcgatcacg ttcggacgac cgataaggat cggtttcgcc agtcccagcg
    42421 ttaccagttc ctgagtcgca tgcagaacgc gcgcctcttc cccttccggc agaactacgc
    42481 gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg gttttgtaaa
    42541 cgaactcagt cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc gtcgccacgc
    42601 ccgactccat cgccgctttt gcgaccgcag gagcgatctt aacgatcaag cgcggatcaa
    42661 acggttttgg aatgatgtat tccggaccaa agctcagatc ctgaacgcca tacgctgaag
    42721 ccaccacttc actctgttct gcatgggcca gttctgcaat cgcgcgtacc gccgccagtt
    42781 tcatctcttc gttgatggcg gttgcgccaa cgtccagcgc gccacggaag atgaacggga
    42841 agcaaagaac gttgttcacc tggttcgggt agtcggaacg gccggtacag atgatggcat
    42901 ccggacgcac ttctttcgcc agcggcggca gaatttccgg ttccgggttc gccagcgcca
    42961 ggatcattgg tgcccgagcc attttcttca ccatttcctg ggtcagcact ttcgggccgg
    43021 aacagcccag gaaaatatcc gcaccttcaa tcacatcatc gagggtacgt ttgccgtcat
    43081 ccaccaccgc atatgcggct ttggtttccg ccatatttgg ctcacggccc tgatagataa
    43141 cgccttttga atcgcaaacc acgatgttat gtttttgcag ccccagcgct accagcaggt
    43201 tcatacaggc gattgctgcg gcacccgcgc cggaaaccac catccgcacg tcggagatgt
    43261 ttttctccac cacgcgcaag ccgttgagga tggcggcagt gctgatgatt gccgtgccgt
    43321 gctgatcgtc gtggaatacc ggaatattca tccgctcgcg cagtttctgt tcaatataga
    43381 aacattctgg cgctttaatg tcttcgaggt tgatgccgcc gaaggtcggt tcgagcgcgg
    43441 cgacgacttc aataaatttg tccgggtcga gttcatcaac ttcaatgtca aatacatcaa
    43501 tcccagcgaa tttcttaaat agaacgccct tgccttccat caccggtttg cctgccagcg
    43561 cgccaatgtt gcctaacccc agcaccgccg taccgttaga gatcaccgca accaggttac
    43621 cgcgcgcggt atatttgtag gcttttagcg ggtctttttc gatttcaaga caaggtgcgg
    43681 caacgcctgg tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc ttggttggag
    43741 aaacctggat tttccctgga actggaaatt catggaaatc aagtgcactt tgttttaact
    43801 ggtcatccat ttggttgttc ctttcacgta acgttcacaa ataaagtgtg tgggcaacag
    43861 cccctgccca caacgtggcg cacattatta ccctgccgga gtctacagac tttgagcaag
    43921 tccaaactct caccattaat ataatgtttt ggtaataatc ctataacact gatgttacct
    43981 gcttaatcca gcaataccat gcctgtctgc tatgcttttt tgatgcgttt agcgaaattt
    44041 ctcagaagtg tgaattaacg cactcatcta acactttact tttcaaggag tatttcctat
    44101 gaacgagtta gacggcatca aacagttcac cactgtcgtg gcagacagct gcgatattga
    44161 gtccattcgc cattatcatc cccaggatgc caccactaat ccttcgctgt tactcaaggc
    44221 tgccggatta tcacaatatg agcatttaat agacgatgct atcgcctggg gtaaaaaaaa
    44281 tggcaagacc caggaacaac aggtggtcgc agcgtgtgac aaactggcgg tcaatttcgg
    44341 tgctgaaatt ctcaaaatcg tacccggttg cgtgtcaaca gaagttgatg cacgcctctc
    44401 ttttgataaa gaaaagagta ttgagaaggc gcgccatctg gtggacttgt atcagcaaca
    44461 aggcgttgag aaatcacgca ttttgatcaa actggcttcg acctgggaag gaattcgcgc
    44521 ggccgaagag ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt tttcttttac
    44581 ccaggcgcgg gcctgtgcgg aagcaggcgt ttttctgatt tcgccgtttg tagggcgtat
    44641 ttatgactgg tatcaggcac gcaagccgat ggacccgtat gtggtggaag aagatccggg
    44701 cgttaaatcg gtgcgcaata tctacgacta ctataagcaa caccactatg aaaccattgt
    44761 gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct gcgatcgact
    44821 gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa gtttctccag tggtacgtaa
    44881 attaatcccg ccttctcaga cgttcccacg cccagcaccc atgagcgaag cggagttccg
    44941 ttgggagcac aatcaggatg cgatggcggt agaaaaactg tctgaaggca ttcgtctgtt
    45001 cgctgttgat caacgcaaac tggaagatct tcttgccgcc aaactataaa ccagccacgg
    45061 agtgttatat gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt atggatgctg
    45121 tacaaaaagc caattctggt catcccggcg cgccgatggg catggctgat attgccgaag
    45181 tgctgtggaa cgattttctt aaacataatc ctaccgaccc aacctggtat gatcgcgacc
    45241 gctttattct ttccaacggt cacgcgtcga tgctgctcta cagtttgcta catctgaccg
    45301 gttacgacct gccgctggaa gaactgaaga acttccgtca gttgcattcg aaaaccccag
    45361 gccacccgga gattggctat acgccaggcg ttgaaaccac caccggcccg cttggacaag
    45421 gtttggcgaa cgccgtcggg ctggcgatag cagagcgtac actggcggcg cagtttaacc
    45481 agccagacca tgagatcgtc gatcacttca cctatgtgtt tatgggcgac ggctgcctga
    45541 tggaaggtat ttcccacgaa gtctgttcgc tggcaggcac gctgggacta ggcaagctga
    45601 ttggttttta cgatcacaac ggtatttcca tcgacggtga aacagaaggc tggtttaccg
    45661 acgatacggc aaaacgtttt gaagcctatc actggcatgt gatccatgaa atcgacggtc
    45721 acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa gataagccgt
    45781 cgctgattat ctgccgtacg gtgattggct ttggttcgcc gaataaagca ggtaaggaag
    45841 aggcgcacgg cgcaccactg ggggaagaag aagtggcgct ggcacggcaa aaactgggct
    45901 ggcaccatcc gccatttgag atccctaaag agatttatca cgcctgggat gcccgtgaaa
    45961 aaggcgaaaa agcgcagcag agctggaatg agaagtttgc cgcctataaa aaggctcatc
    46021 cgcaactggc agaagagttt ccccgacgga tgagcggtgg tttaccgaag gactgggaga
    46081 aaacgactca gaaatatatc aatgagttac aggcaaatcc ggcgaaaatc gctacccgta
    46141 aggcttcgca aaatacgctt aacgcttacg ggccgatgct gcctgagttg ctcggcggtt
    46201 cggcggatct ggctcccagc aacctgacca tctggaaagg ttctgtttcg ctgaaggaag
    46261 atccggcggg caactacatt cactacgggg tgcgtgaatt tggcatgacc gctatcgcca
    46321 acggcatcgc gcaccacggc ggctttgtgc cgtataccgc gacgttcctg atgtttgttg
    46381 aatacgcccg taacgccgcg cggatggcgg cactgatgaa agcgcggcag attatggttt
    46441 atacccacga ctcaattggc ctgggcgaag atggtccgac gcaccaggct gttgagcaac
    46501 tggccagcct gcgcttaacg ccaaatttca gcacctggcg accgtgcgat cagatggaag
    46561 cggcggtggg ctggaagctg gcggttgagc gccacaacgg accgacggca ctgatcctct
    46621 caaggcagaa tctggcccag gtggaacgta cgccggatca ggttaaagag attgctcgtg
    46681 gcggttatgt gctgaaagac agcggcggta agccagatat tattctgatt gccaccggtt
    46741 cagagatgga aattaccctg caagcggcag agaaattagc aggagaaggt cgcaatgtac
    46801 gcgtagtttc cctgccctcg accgatattt tcgacgccca ggatgaggaa tatcgggagt
    46861 cggtgttgcc ttctaacgtt gcggctcgcg tggcggtgga agcaggtatt gccgattact
    46921 ggtacaagta tgttggtctg aaaggggcaa ttgtcgggat gacgggttac ggggaatctg
    46981 ctccggcgga taagctgttc ccgttctttg gctttaccgc cgagaatatt gtggcaaaag
    47041 cgcataaggt gctgggagtg aaaggtgcct gatggtgatc gccggatgct gtatgttccc
    47101 ctcaccctaa ccctctcccc agaagggcga ggggacggtc cgtgcgcttt ttagggccgc
    47161 atctgcgatt ttgtttaacg ggtgatccac aacgtgggcc tggcgtctgg cccatgccag
    47221 ttatcgcagg taggttctgc ggcgtaacgc accaggcgaa aacgctgacc gtcaaagcgc
    47281 cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt caccagttca
    47341 cgcgatttct catcaaatgt tgcgttcatc agttccagtt cattcgtctc ctggccgttg
    47401 ttgaacggca aacgcaaccg aaccgggcgc gaggctagcg gctttttacg cgacacaatc
    47461 catgccaaat caatggtgtt ataggcccct gcctcacagc taatcatcat cagtgcttta
    47521 tcatcggtca gcgcagtgac attcacctca cgacgtaacg gatcaagcga gcagcgcaga
    47581 ccattcatcc gccagtttcc ataatccagc aaatcgttac gttcttcgag tgagagtggc
    47641 gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg tacgctgaga
    47701 ggcggttcgt cccctttctt gatccacgcg gtttcactgc caacgcgctt ttgctgagca
    47761 tcaataaaca acaacgccgc ttttaagccg ctcagagaaa tggtctgatt gccgtcgcgt
    47821 aaggtgatcg ccttcccttc ctgaattatc tgcaaaaacg cggtgatggt tgccgtatca
    47881 tcggttacta ataaccatgg tgaaatccgc cacttgtcac cacttaatgc caacggctca
    47941 ccatctaata gcagccgtgg cgctatctcc ccttcagacg catccggcga cttcaaccca
    48001 ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccag cgctgcggct cagggtcatc
    48061 accagtccat tatgatcgcc cgtattgcgc gccacacaaa aattttgatt attacaggtg
    48121 acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga cgtcggcaac
    48181 aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg acggtacgac cccagaagtt
    48241 caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcggatttgt
    48301 cggattgagc caataataaa caacgaaata ttattgctat ttttcagtcc attaaatgtg
    48361 acatttgcaa atagttaagc aataacaccg tcttaccgtc acgtatctcg ccggttttga
    48421 tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca tcttcaacac
    48481 cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag tggattagct
    48541 cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg cccacttcat
    48601 agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg ttatccagca
    48661 gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag gtagcgacac
    48721 ggaactgacg aatcagaacc acgctctttt tcttcgcgtt gtacaggagg atcgtcgcac
    48781 cattaccgcg atcgtaaact tcacgtttat ggcggataac ttcgccatct ttgcgcgtga
    48841 gatcgtaagt aatgttgtgc agggtgaaat agttatcgga gagaattttg tctttaatga
    48901 gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat attacgctgt
    48961 gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac ccccagccag
    49021 tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac cagatccgca
    49081 ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta acggtactgg
    49141 ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg cgactccagc
    49201 cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc tggcatgaca
    49261 aattcactgc cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg ctcgccaaga
    49321 cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct cattcagctc aagcgccacc
    49381 ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcttcac ctctttcttc
    49441 gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact cgcgccgtgg
    49501 cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac cacgacgtta
    49561 agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagccccat cacctgttta
    49621 gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc attgggtaaa
    49681 cccgctttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa
    49741 agcaaatcca aagagacatc tttacccact tcacaattga gttcgaagtg aatccccatc
    49801 gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa agaaggaatg
    49861 ccgaaagtga gcaagccacc gatttctgga tggcgatcgt acaccgtcac cgccacgcca
    49921 ttgcgggtca gaacatccgc acaggccagc cctgccggac ctgccccgat aatcgccacc
    49981 cgcttgtcca ctttggtgac atgacttaag tcaggacgcc aacctttcgc caacgcctga
    50041 tctgaaatat agcgttcaat gttgccgata gttaccgcgc cgtgctcatc gcgaatagta
    50101 caggcacctt cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc cggcagggtg
    50161 ttggtctggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac cagttcaatc
    50221 cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg ctcgccgaac
    50281 ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag ataaatttca
    50341 tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt
    50401 gcctgcattt gttcgacttt actcattacc ggcatttctt gtgcagcggt actggcatgc
    50461 cacggttgat gctcctgacg tgcggtgcgc aagcggcggg atttcgccat gccggagagt
    50521 gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca ggcaggaccg
    50581 ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg
    50641 acgggtgtca ggacgatttg catcgtacca aacggacagg ccaccacgca ggatttacag
    50701 ccaatacact tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat tgcgccatta
    50761 gggcagctac gggcgcaggg cgcatcttca caatggtgac aggtcactgc actacgttgc
    50821 tgctgatgtt tgataaccgt aattcgggga tgaaaatggt gttggctcag gacatgttgc
    50881 tcatcattgt gagccatgac acaggcgatt tcacaagcat gacaacccag acattgctga
    50941 ctgttggcca taataaaacg attcataacg accttctttt ttggttgtaa aaaccttatt
    51001 ctttatatga gtgttgttat tacccgactt acaggggaac cggcaatgtt catgtgccca
    51061 gaataagtaa ctatttcgct ataaactgtg gcagatcaaa taatcccatc actgactaaa
    51121 ttgcgtttca gcgaactgga acattaatga ttttttgtgg agaagacgcg tgtgattgtt
    51181 aaacgacccg tctcggccag tctggcccgg gccttttttt acattgtgct gctgtcgatt
    51241 ctttccacgg gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc
    51301 aatattgccg gatcgctgcg tatgcagagt taccgcctgg gctacgactt gcaaagtggc
    51361 agtccacaac tcaatgcaca tcgccagttg tttcagcagg cactgcattc accggtatta
    51421 accaacctca acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat
    51481 gccaactggc tggagatgaa taatcggctc agcaagggcg atttgccgtg gtatcaggcc
    51541 aatattaata attatgttaa tcagatagac ctgttcgtgc tggctttaca gcactacgct
    51601 gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg gcggtatcgg tattttcacg
    51661 ctggtctttt ttactttgcg ccgcatacgc catcaggtgg ttgccccgct gaatcagctg
    51721 gttaccgcca gtcagcgtat tgaacacggg cagttcgact cgccgccgct ggataccagt
    51781 ctgccgaatg agcttggtct gcttgcaaaa acctttaacc aaatgtcgag cgagctgcat
    51841 aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct ccacgagacc
    51901 aagcgtcgtc tggaggtgtt gtatcagtgc agtgttccca ggcgctgaac accagccaga
    51961 ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa gcagctgaat
    52021 atctggagct gaacgttggg gataactggc ggattagcga agggaagcca aaccctgagc
    52081 tgccaatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa ctgcactggc
    52141 aaaatagtca cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg atgctgggac
    52201 gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac
    52261 gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtgctttct tacttacgta
    52321 tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca
    52381 tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta
    52441 ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata
    52501 cgttacaaaa tcaaaccagc gctaaactga ccctcgactg ccgtctgcca accctggcac
    52561 tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga
    52621 tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg gacggcaatc
    52681 acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa cccgaaggtc
    52741 attatggact gaatatcatg cgcgaacgcg cggaacggct gggcgggacg ctgacttttt
    52801 cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctccgca gagggtgagg
    52861 ggaaggggaa cacgccggct gaggtgtaaa taattaccct cacaccggcc ctctcccttt
    52921 aaaggcgaga gggaaaaacg tgcccatatt gacctgtaag cgagtgtgtc agcaatctca
    52981 tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg ccgctacagt
    53041 gaagcaagtc aagcctacaa caatacgcag aaacacgagg tcctctttta atggcgaatt
    53101 tctttattga tcgccccatt tttgcctggg tgctgacaat cctgttgtgt ctgacaggta
    53161 ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca ccgaatgtgc
    53221 gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc gtgacccagg
    53281 ttatcgagca aaatatgacc ggcctcgata atctgatgta tatgtcatct cagagcagtg
    53341 gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg gacgaagccg
    53401 tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag gcggtgcaaa
    53461 atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt gccttcgtct
    53521 ctaccgatgg ctcgatggat aaacaggata ttgctgatta tgttgccagt aatattcagg
    53581 acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg caatattcca
    53641 tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct aaagatgtca
    53701 ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt ggtacacctt
    53761 ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg caaacaccag
    53821 aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta aggctgggcg
    53881 atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc ttcaatggta
    53941 agccagcctc cgggttgggg gtaaaactgg cctccagcgc aaacgaaatg gcgacagcgg
    54001 agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcacgga ctggaataca
    54061 aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg gtgaaaacgc
    54121 tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa aacttccgcg
    54181 ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc tccgtacttt
    54241 acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg gcgatcggtc
    54301 tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga acgtattatg agtgaggaag
    54361 gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg gcactggtcg
    54421 ggattgcgat ggtgctttcg gcggtatttg taccaatggc cttcttcggc ggcaccaccg
    54481 gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg tcagtactgg
    54541 tagcgatgct cctcactccg gctctgtgtg ccacgctact taagccactg aaaaaaggtg
    54601 agcatcacgg gcaaaaaggt ttttttgcct ggtttaacca gatgtttaac cgcaacgccg
    54661 aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg attgtgattt
    54721 atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg tcgttcttac
    54781 cgctggaaga ccggggcatg tttactacct cggtacaatt gcccagcggt tcaacccaac
    54841 aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat gaaaaagaca
    54901 acatcatgtc ggtgtttgcc accgttggtt ctggccctgg gggtaacggg caaaacgtgg
    54961 cgagaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc ggcacctcgt
    55021 ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct cgcgttatcg
    55081 ccagcagccc accagcaatt agcggtcttg gtagttctgc aggttttgat atggagttgc
    55141 aggaccacgc tggagcgggt cacgatgcgc tgatggcagc acgtaatcag ttgctggcgc
    55201 tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgat gacagtccgc
    55261 agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgat atcgacgata
    55321 ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt atggatcgcg
    55381 gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg ccagatgaca
    55441 tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct gctttcgcga
    55501 cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta taacggttat tctgcggttg
    55561 agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat attatggaat
    55621 cgttagtgaa gcagctgcca aacggctttg gtctggagtg gacggcgatg tcgtatcagg
    55681 agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg gtggtattcc
    55741 tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg ctggtcgtgc
    55801 cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa aacgacgttt
    55861 acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc atcctgatcg
    55921 tcgagtttgc taacgagatg aaccaaaaag gccatgacct gtttgaagcg acgctccacg
    55981 cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc ttcggcgtat
    56041 tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg ggtactggcg
    56101 taatgggcgg gatgatttca gctactattc tggctattta cttcgtgccg ctgttctttg
    56161 tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata aaaaaggcga
    56221 catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc gggttcttgc
    56281 tgtttatact ttgtctcagg aattacttac gaagcataac ttcgataaag tctttccagt
    56341 tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt ctacggaaac
    56401 aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc gggggacaaa
    56461 ccttatgtaa atactatggt cctgcggtga taatttgtat gtgatacaca gcaacatttc
    56521 gagatattca tacggcatct aatactgatt taattctggt taaaatacag acagataaca
    56581 agatgaatat tcttaatgtt tacgttaaaa atgtttaata ttatttaata gttgttaatt
    56641 tgaatacttc gataatgtta tattttctga taatcatttg caggcaaaat gttttcaccc
    56701 ttaaatgagt atttattctc ataaatcgaa aaaggattca ttatggttac actttacggc
    56761 atcaaaaatt gtgacaccat taaaaaggct cgccgttggc tggaagccaa taacatcgac
    56821 tatcgttttc atgattaccg cgtcgatggg ctggacagcg aattattgaa cggttttatc
    56881 aacgaattag gctgggaagc gttactcaac acccgtggta caacctggcg taaactggac
    56941 gaaactaccc gcaataaaat caccgatgcg gcctctgcgg cggcattaat gactgaaatg
    57001 cctgcaatta tcaaacgtcc attgctctgc gcgcctggta agcctatgct gctgggtttc
    57061 agtgattcca gttatcagca atttttccat gaggtgtagt ctatgtcgtg cccggttatt
    57121 gagctgacac aacagcttat tcgccgccct tccctgagtc ctgatgacgc aggatgtcag
    57181 gctttgttga ttgaacgttt gcaggcgatc ggttttaccg ttgaacgcat ggattttgcc
    57241 gatacgcaga atttttgggc atggcgtggg caaggtgaaa cgttagcctt tgccgggcat
    57301 accgacgtgg tgccgcctgg cgacgccgat cgttggatca atccgccgtt tgaacccacc
    57361 attcgtgacg gcatgttatt cgggcgcggt gcggcagata tgaaaggctc gctggcggcg
    57421 atggtggtag cagcagaacg ttttgtcgca caacatccca accatacggg gcgactggca
    57481 tttctgatca cttctgatga agaagccagt gcccacaacg gtacggtaaa agtcgtcgag
    57541 gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg tcggcgaacc gtcgagtatc
    57601 gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat cgttaacctg caacctcacc
    57661 attcatggcg ttcaggggca tgttgcctac ccacatctgg ctgacaatcc ggtacatcgc
    57721 gcagcacctt tccttaatga attagtggct attgagtggg atcagggcaa tgaattcttc
    57781 ccggcgacca gtatgcagat tgccaatatt caggcgggaa cgggcagtaa caatgttatt
    57841 ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca ccgaactgac tgatgagatg
    57901 atcaaagcgc aggtacttgc cctgcttgaa aaacatcaac tgcgctatac ggtggattgg
    57961 tggctttccg ggcagccatt tttgaccgcg cgcggtaaac tggtggatgc ggtcgttaac
    58021 gcggttgagc actataatga aattaaaccg cagctactga ccacaggcgg aacgtccgac
    58081 gggcgcttta ttgctcgcat gggggcgcag gtggtggaac tcgggccggt caatgccact
    58141 attcataaaa ttaatgaatg tgtgaacgct gccgacctgc agctacttgc ccgtatgtat
    58201 caacgtatca tggaacagct cgtcgcgtga tgagtggttc tgcaagagga aataagcatg
    58261 gactggctgg ctaaatattg gtggattctg gtgattgtct ttttggtagg cgtcctgctg
    58321 aacgtgatta aagatctcaa gcgcgtcgac cataagaaat ttctcgccaa caagccggag
    58381 cttcccccgc atcgtgattt caacgataag tgggacgatg acgacgactg gccgaaaaag
    58441 gatcaaccga agaagtaagc tgagtgctca accgtaggcc ggataaggcg ttaacgccgc
    58501 atccggcaat ggtgaacgat ccctgatgcg acgctcacgc gtcttatcag gccaggtaat
    58561 ctgtctttta catcatttca atcacatcat catcgccagg cttaccgcca cttagcgctt
    58621 ggtcgaaata atgtttcgga atggtataac gcaagtgatc cagggcaaat tgcatactgc
    58681 gattgtcgat cgcatggcct aaatcctcca cgatatccag cgtcacgtca ccaccggcac
    58741 tgattaacgc ttcctgcgcc gcaactgcat gcgccagatc gataaccgga tcttcaccac
    58801 catgaatcaa gtgaattgtc gtggctgtag acgcggtctc cggcaggctc gcatagcgtc
    58861 cgttaaaggc aatgacgcgc gaagcaaggc ccggttcggc tttaatgctc tccagcgcca
    58921 taatcgcgcc ttgtgagaaa ccaattagcg ccgtggcgtt agcccctact ccgctctgtt
    58981 tctgccagta gcgtaccgtt tcaataaacg tcggcatgat ggcatccaca cgcgcctggc
    59041 gattatcttc cgtaataccc tgcactgaaa accactgacg ccccgccgga ttaccgctcg
    59101 gctccgcgcc accgacactc accaccagcg catcaggaaa cagcggtgca aaccagctgc
    59161 ctatttcccc cattgccacc gggttatccc cgacaccgtg gaaaagcagt aacaactgtt
    59221 gtgcaggttt atccgggctt tgaacaacaa aatggtcatg tttcatggcg aactccttaa
    59281 ctgatgtcat taattttacg ccgctgactc acaatgacca tgggagaaaa ttgaaggagt
    59341 cagtgaaaaa attgccattg cgttatgcga tcgcgcagac gctcagtgcg aacctcattt
    59401 agtgcgaaca gcgcctgcgc ggcctcttcc cgctggcgaa ccagtaacat cttgcggcct
    59461 gaaagtttaa gtgtggtaca taactgcgcg tcactggcgt ttttctgtaa acgcccacgc
    59521 agcgccggta atgccagttc actggtttgt agcagacgca ataagcaacc taacgatgtt
    59581 aatagcggac gatgagcgaa agcaaaaccg gccagttcaa gccagtcgtc gtcagaaagg
    59641 atggcatcgt ttagtggatc aacaggaagc atttcgccat tccactgcgc gagagcttgc
    59701 gcatcgcgac gtaaacggta atgctcacgt tcagccagct gtttacccgc atcactcatc
    59761 ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt cccgatgatt acccatccgc
    59821 accagcacaa aaccgcagcg ttgccagaaa cgccataact ccccagtgta accaaaactc
    59881 accgaaagat agtcgaggtc atgcgtatat tgcaaagctc cagcaataag ttgtcgccct
    59941 gtgccttccc gctgacgagc cggatgaact gctatccggc taacccgcct tccgcgcaat
    60001 gtcgccgcca gtggattgct gccgtgcgcc gccagcgact gggccaccag attaccccgc
    60061 gggcgacgaa aacctgccca taccgcctga ctgagttctt gagataatcc gccctcatcc
    60121 accagccaca acgccccggc aatctcgttt tcgccagccg cctgtaaaaa atgttgccct
    60181 ggtgcatcca tcatccggcg taaatccagc ggcgaggtcc ggtagtgcgc accagacaat
    60241 aactgataaa cctttaacgg cgtttctggc tcgcttcgcc ataacgtttg ttcaaatgcg
    60301 gaaatgacga tattgccttg tggtgtatgg gtgaagtttt catcgttaaa aaccagtgcc
    60361 tcactaacca ttttttccag cgggcatccc tgtgcccagc ggatcggctg ttgcagttca
    60421 aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca aaaaaccacg tccggtacct
    60481 tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa aacgcgatac cagttgatgc
    60541 aacaacggcg caggtatggc tgcggcttcg tcgaccacca gccagtcggc ttgctcatcg
    60601 ctggctaaca aggcatccgg tgcaataaag cgaaacttct cgcccgcaaa ttgtgccagt
    60661 acatccgttg ccgcttttgc gggcgcggtg acaatcgcac tgcccgcaat acgagaaatg
    60721 agttgccctg ccaacgccga cttaccgcgc ccacgcgcag ccgttaccgc tgccacgccc
    60781 ggcggcatgg tcagtagctg ctgtaagagt tgctgttgtt ctggttgtgg tgcgccggtc
    60841 gcggggtgcc agtcagtacg gggagtaaaa tgcgccaacg agaacggctg gttttgccgc
    60901 cagaggatag cgtcgttatc cgccgtaagt acgcgtttga gatgctggac aaaatgcggc
    60961 gtcgcaatag ggtcagggca atcactccag cgcagcgagt cggcatcagg ttggttttcc
    61021 cactcttccc atacagggag taacaaaacc agccagcttc ccgctttcaa cgttccgcta
    61081 agtgctgcaa aggcagcggc atcaaagccg tggcgggcgt cgaataccgc atgccggaac
    61141 tcgcgcccaa gtaaagtttg tagcgccgag ggtgaacagt ggttttcagc atctagctgc
    61201 ggcgaaatcc acagccagtc gccaggtaag gcatcacgca acttaagagc atgatcaaaa
    61261 caccaaccct cttccccgct caacaccagc aagcggcaga tcccttcacg tttcatttgc
    61321 gctgttaatg tgtaaagcgc agtcagttca gccatccctg ccccggaaat taaatgcttt
    61381 taccaaaagt attgcattgt gccggatcgc cgctgtcgaa accacgttta aaccagctgt
    61441 agcgttgctg agaagtgcca tgtgtgaaac tgtctggtac tactcgcccc tgactttgct
    61501 gttgtaaacg gtcatcgccg atggcctgcg ccgcattcag cgcctcttcc agatcgccag
    61561 tttccagaac gccttgctgc tgcatactat gcccccagac accggcaaaa cagtcggcct
    61621 ggagttccat acgcacagat aagcggttta cttccgcctg cgtcgcgttt tgttgcagtt
    61681 gacgaacttt cggctcgatg cctaacagtt tctgcacatg atgaccgact tcatgggcga
    61741 taacgtaccc ctgggcaaaa tcgccatccg cgccaagttt gtctttcatg tcatcataga
    61801 aggagagatc gatataaact gtgccatccg ccgggcaata gaacggcccc ataatggact
    61861 ggcccgcccc gcagccggtg cgcgtcattc cacggtacat gaccagcttc ggttgctgat
    61921 aggtcttacc catcttttcg aactgttgtc cccaggtgtc ttccgtggtt gcctgaatca
    61981 ccgaggtgaa ttttgcggct tcgtcttcat tagggctaat tgaccgcgtt gattgttgtt
    62041 gagaaaccgg ctgcccggtc atcaacccgg ttaaatcaac gccatagtag cctgcaacca
    62101 gcacgactat cagtaaaatc agcccgcctt taccgctcgg cagacggaaa ccgggaccgc
    62161 ccattgacgg accgccagag ctgttgcgcc tgtcttcaac attgtcactt tcacgtcgcc
    62221 cttgccaacg catagatacc tcaacaatat attcattata gaaatgatcg taggtggttc
    62281 agaggaagat taccacagga aagcgggatg aaatggcgga ggaatgagcg ttcaatgcat
    62341 atattccaag agcggttgtc ttgccgtgcc agctgcacgg caagatgatg aacagaaaaa
    62401 tcagtccagc tgtacaccca ggcggcgggc gacggcttca taggcttcga tcaggccacc
    62461 gaggctctgg cggaaacggt ctttgtccat tttctccagt gtttctttgt cccacaggcg
    62521 gctgccgtcc ggggagaact catcgcccag taccacttca cctttgtaca gaccaaactc
    62581 cagcttgaag tcgaccagaa tcagaccagc atcatcgaac agttttttca gcacgtcgtt
    62641 cgctttgtag gtcagctctt tcatacgcgc caggttctct ttgctcaccc agccaaaggt
    62701 ttcgcagtaa gattcgttga ccatcggatc gtgcatggca tcgtttttca ggaacagatc
    62761 gaacagcggc gggttcagct caataccttc ttcgattcca agacgtttca ctagagagcc
    62821 agcagcacgg ttacgcacga cacactcaac cggcaccata tccagctttt tcaccagaca
    62881 ttcggtatcg gagagcagac gctccatttg agtcgggata cccgcttcag ccagtttgct
    62941 cataatgaag tagttgaact tgttgttcac catacctttg cgatcaaact gctcaatgcg
    63001 cgcgccatcc cctgctgacg tatcattgcg gaattcgagc accaacaggt ccgggttttc
    63061 cgtgctgtat acggttttcg ctttaccacg atacaactca gcttgctttt gcatctttat
    63121 cactcctggg tgtgaattaa cgttttaaaa tcttttgctg tctggtgtgc cggatgtttt
    63181 gtcggatgcg gcgtgaacgc cttatccgaa ctacgtccga caaacaaatt tcgtgcgaat
    63241 taccgctaaa atcgcgtttt cctgccgacg cacacgtttg cgtatcatat cagaaaaaag
    63301 ggccggatga ttccagccct gtatttttac ttgctaaacg cagcctggaa gacagctacc
    63361 agcgcgtcgt tctgactctg agtcagagta tgacctttcg gatcgatgaa ctgtaggctg
    63421 ctgcggttat ctaaatcgcc aacctgcagt ttatagtcac cggatgccag gcctggatcg
    63481 ctcgcgccca gttcctgcca gtcgctgtca gacagcggct tataagttac ggccatgttg
    63541 ccctgcgaac gggtgctgtc ggtcactttc atgcccactt tttccagcgc cgctggcaga
    63601 cgttgccaaa ccacattgaa cggcccgcgt acgaccagca ttggtaaacc ggtgtcatca
    63661 gctgcacttt gtacgtccat agtggtggag gcacgatttt gcgcagcgtt cgcggcgtca
    63721 gtggcagatt tatccagacc ggcggaaata acgttcatca tctccgtgct gtaacgctgc
    63781 atggaagccg cgtctgcaac cggtttgccc gcctgttcca ggttcagcag tttaaccgta
    63841 accgcctgct gataaccctg cggcttaaca gagatttgat aacgaccacg atactgctcg
    63901 tcttcgtcca gacggttcca ttgtacccaa tcggtggtca gtgtctgacc agcatcatca
    63961 cgttgggtga tggtgtagtt tttcgcctgc agcacgctaa ccacctgcgg ccacagagta
    64021 ttgccacgac cattttccac cagcaatgaa gcggtatcgc ccgtgaactg ggtacgcgcg
    64081 ccagaaacca gtgccaacgg ctgggctggt ggacgaatgt ccagcgcctt accgacagca
    64141 ccactaccgt tggtcaccgg gattgcataa tcaccggagg tcaccggcaa aatcattcca
    64201 gccggggcat gaagctccgc aagcggtgcc gcttccaggt aggcttcatc accactgacc
    64261 tgacgcttat agcgtgagtc agaactacag gcagcgagta ataaaacaag cgaaacaccc
    64321 gcaacctttg ccaggcgcga cttttgaaca gagtaagcca tcaaatctcc ctaaacttta
    64381 cagcaaaccg gcatgcttaa gcgccgctct gaccgtctca cgaccactgt cggtgattgg
    64441 tgtcattggc aggcgcagcg tatcggtcgc cacaagaccc agttccttac atgcccattt
    64501 caccgggatt ggattgggtt cgacaaatag tttgttgtgt aatggcatca gacgctgatt
    64561 aataacgcgt gcctcggcaa aatgcccttc tgctgccagt ttgcacatct gggccatatc
    64621 acgcgctgcg acgttagccg taacggaaat aactccatga ccgcccaatt gcatgaagtc
    64681 cagcgcgctc gcatcatcgc cgctcagcag aacaaaatca tctgaaacca gctctttgat
    64741 ctggtttaca cgcgttaagt tccctgttgc ctctttgatt ccgataatat tttttacttt
    64801 cgccagacgg cccaccgttt ccgggagcag atcgcagcca gtacgggacg gcacattata
    64861 cagaatttgc ggcaggtcag tatgctcagc gatggctttg aaatgctgat acaaaccttc
    64921 ttgcgacgga cgattgtagt aaggggttac cgtcaggcag ccgacgatac cactgtcatt
    64981 gaagcgctgc gtcaggctaa tggcttccgc agtagcgtta gcgccggtcc cggcaattac
    65041 cggaatgcgc ccatcagcca gatccagcgt catcatcacc acatcagcat gttcgtcatg
    65101 atttaaggta gcggactcgc cagtggtgcc aacagaaacg atcgccgaag taccgctggc
    65161 gacatgataa tcaatcagtt ttttcaagct agcccgacag acattacctt tttcatccat
    65221 cggagtaaca atcgcgacaa tacttcccgt gaacatgggc catcctctgt gcaaacaagt
    65281 gtctcaatgg tacgtttggt atggcattaa aagcaagcag acagaaccgt tctgattgtt
    65341 gtatgcatga tttttttatg ctttccttaa gaacaactca ccccttaaag gaataaccag
    65401 tttgacactg tcatcgcaac attatctggt gatcactgcg ttgggtgccg atcgccctgg
    65461 aattgtgaac accatcaccc gtcatgtcag tagttgcggc tgtaatattg aagacagtcg
    65521 cctggcgatg ctgggagaag agttcacgtt tatcatgctg ctttccggtt catggaatgc
    65581 catcactctg attgaatcaa cgttaccgtt gaaaggtgcc gaactggatc ttttaatcgt
    65641 gatgaagcgc acgacggcgc gtccgcgtcc gccaatgcca gcatctgtct gggttcaggt
    65701 cgatgttgca gactccccgc atttaattga acgcttcaca gcacttttcg acgcgcatca
    65761 tatgaacatt gcggagctgg tgtcgcgcac acaacctgct gaaaatgaac gggctgcgca
    65821 gttgcatatt cagataaccg cccacagccc cgcatctgcg gacgcagcaa atattgagca
    65881 agcgttcaaa gccctatgta cagaactcaa tgcacaaggc agtattaacg tcgtcaatta
    65941 ttcccaacat gatgaacagg atggagttaa gtaatgaatc cactgaaagc cggtgatatc
    66001 gcaccgaaat ttagcttgcc ggatcaagac ggagaacaag ttaatttgac cgacttccag
    66061 ggacagcgtg ttctggttta tttctacccg aaagccatga cccccggctg taccgtacag
    66121 gcctgcggct tacgcgataa catggatgag ttgaaaaaag cgggcgttga tgtgctgggt
    66181 atcagcaccg ataaacccga aaaactctcc cgttttgcgg aaaaagagct gcttaacttt
    66241 acgctcctgt ctgatgagga ccaccaggtg tgcgaacaat tcggcgtctg gggtgaaaag
    66301 tccttcatgg gcaaaaccta cgatggtatt catcgcatca gcttcctgat tgacgctgat
    66361 ggcaaaatcg aacatgtctt tgacgatttc aaaaccagca atcaccacga cgttgtgctg
    66421 aactggctga aagaacacgc ctgattactt tgctccattc cgtgctggct gcgcttgcgg
    66481 ccagcatacc tcacttctcg tgatcaagat cacattctcg ctttcccctg cgacacgggt
    66541 gtcgaatcca ttttttgctg aacgttaatg accatcattt ttgtaccgtt cagaatccag
    66601 ttaatacata acttattgaa tagattgagt taatcagaat ggcatccttt atacaatatg
    66661 aaatgcaatg tttcatatca ttttcaagga gccgacatga accgctttgt ggtggccgaa
    66721 ccactgtggt gtacaggatg taatacctgt ctcgctgcct gttcggacgt gcataaaacg
    66781 caaggtttac agcaacaccc gcgcctggcc ctggcgaaga cgtcaacaat cactgcccct
    66841 gtcgtgtgtc atcactgtga ggaagcccct tgcctgcagg tctgcccggt caatgccatc
    66901 tctcagaggg atgatgcgat ccaactcaac gaaagcctct gtattggctg caagctttgc
    66961 gccgtggtct gcccatttgg cgcaatcagc gcttcaggaa gccgtccggt gaatgcccat
    67021 gcgcaatatg tttttcaggc tgaaggctca ctcaaagacg gcgaagaaaa cgtgccgccc
    67081 caacatgcgt tgctgcgctg ggaaccgggt gtccagaccg tcgcggtgaa atgcgacctg
    67141 tgtgatttct tgccagaagg tccggcctgc gttcgcgctt gcccgaatca ggcgttacgg
    67201 ctgatcaccg acgatagcct gcaacgtcag atgaaagaaa aacagcgcct tgccgcaagc
    67261 tggtttgcca atggcgggga gtctcccctt tccctcactc aggagcaaca ctaatggatg
    67321 ccctgcaatt attaacctgg tcgctgatcc tctatctgtt tgccagtctg gcttcgctgt
    67381 ttttactcgg tctggacaga ctggctatta agctttccgg catcacatcg ctggtgggcg
    67441 gcgtgattgg catcatcagc ggaattacgc aattacatgc tggtgtaact ttagtcgccc
    67501 attttgccac cccttttgac tttgccgatt taaccctgcg aatggatagc ctctcggcat
    67561 ttatggtgct agttatctcc ttgctggtgg tggtttgttc gctctattca ttgacttata
    67621 tgcgcgaata cgagggcaaa ggcgcggcgg cgatgggctt ctttatgaat cttttcatcg
    67681 catcgatggt tgccctgctg gtgatggaca acgctttttg gttcatcgtg ctgtttgaaa
    67741 tgatgtcgct gtcttcctgg tttctggtca ttgccaggca ggataaaacg tcgatcaacg
    67801 ctggcatgct ctactttttt atcgcccacg ccggatcggt gctgattatg atcgccttct
    67861 tgctgatggg gcgcgaaagc ggcagcctcg attttgccag tttcgcacgc tttcactttc
    67921 tccggggctg gcgtcggcgg tgtttctgct ggcctttttc ggttttggcg cgaaagccgg
    67981 gatgatgccg ttgcacagct ggttgccgcg cgctcaccct gccgcaccat cgcacgcttc
    68041 ggcgttgatg tctggcgtaa tggtcaaaat cggtattttc ggcatcctga aagtagcgat
    68101 ggatctgctg gcgcaaacgg gtttgcctct gtggtggggc attctggtga tggcgatcgg
    68161 cgcaatctcc gcgctcctgg gcgtgctata tgcgctggcg gaacaggata tcaaacggct
    68221 gctggcctgg agtaccgtcg aaaacgtcgg cattattttg ctggcagtcg gtgtggcgat
    68281 ggacggtctg tcactgcacg acccgctgct caccgtggtt ggactgctcg gcgcactgtt
    68341 tcatctgctc aaccatgcgc tgttcaaagg gctgctattt ctcggcgcgg acgcgattat
    68401 ttcgcgtttg catacccacg acatggaaaa aatgggggca ctggcgaaac ggatgccgtg
    68461 gacagccgca gcatgcctga ttggttgcct cgcgatatca gccattcctc cgctgaatgg
    68521 ttttatcagc gaatggtaca cctggcagtc gctgttctca ctaagtcgtg tggaagccgt
    68581 agcgctacaa cttgcgggtc ctattgctat ggtaatgctg gcagtcactg gtgggctggc
    68641 agtaatgtgc ttcgtcaaaa tgtacggtat tactttctgt ggtgcgccgc gcagtacaca
    68701 cgctgaagag gcacaggaag tgccaaatac gatgatcgtc gccatgctac tgctcgcggc
    68761 actctgcgta ttaattgcgc ttagtgccag ttggctggca ccgaagataa tgcatattgc
    68821 ccatgcgttt accaataccc ctcccgtcac tgtcgccagc ggaatagcac ttgtacccgg
    68881 cacgtttcat acacgggtca ctccttcatt actgttgctg ttactactgg cgatgccttt
    68941 gctgccaggc ctttactggc tgtggtgtcg ttcgcgccgc gcagcgtttc gtcgcacagg
    69001 agatgcctgg gcatgcggct acggctggga aaacgcgatg gctccgtcag gcaatggcgt
    69061 gatgcagccg ctgcgtgtgg tcttctctgc gttatttcgt ctacgacaac agctcgaccc
    69121 tacgctgagg ctaaataaag gtcttgcgca cgtcaccgcc agggctcaga gcacagaacc
    69181 cttctgggat gagcgggtga tccgccccat cgtgagcgcc acccaacggc tggtcaaaga
    69241 aatacagcat ctgcaaagcg gcgactttcg tctctattgc ctgtatgtgg tcgccgcact
    69301 ggttgtgctg ctaatcgcta ttgccgtcta aggaaatcac catgagacaa actctttgcg
    69361 acggatatct ggtcattttt gcgttagcac aggccgtgat tctgctgatg ctaaccccac
    69421 tttttacggg tatttcccgg cagatacgcg cgcgtatgca ctcccgccgc gggccgggga
    69481 tctggcagga ttatcgcgat atccacaaac tgtttaaacg ccaggaagtt gcgccgacat
    69541 cttcaggtct gatgttccgc ctgatgccgt gggtattaat cagcagcatg ctggtgctgg
    69601 cgatggcctt accactattt attaccgttt ccccttttgc gggcggcggc gatctgatca
    69661 cccttatcta tcttcttgcc ctgtttcgtt ttttctttgc tctttccggg ctggataccg
    69721 gaagtccgtt tgcgggagtc ggtgccagtc gcgagttgac gctcggcatt ctggtcgaac
    69781 caatgcttat tctctcactg ctggtattgg cgctgatagc aggttccacg catatcgaga
    69841 tgatcagcaa tacgctggct atgggctgga actcgccgct aaccaccgta ctggcgttac
    69901 tggcctgtgg ttttgcctgc ttcattgaga tgggaaaaat tccctttgat gttgctgaag
    69961 cagaacagga attacaggaa ggcccgctga ccgaatattc cggtgccggg ctggcgctag
    70021 cgaaatgggg gctggggctg aaacaggtcg tgatggcagc actgtttgtg gccctgtttc
    70081 tgccctttgg gcgcgcgcaa gaactttctc tcgcctgcct gctgacttca cttgtcgtta
    70141 cgctgctcaa ggttttgctg atttttgtac tggcctcaat cgcagaaaac acgctggcac
    70201 gcgggcgttt tttactcatt caccatgtga cctggcttgg cttcagcctt gctgcgcttg
    70261 catgggtctt ttggttaacc ggtctgtaag gagcactgac ggaatatgga aaatcttgct
    70321 ctgacgacgt taatgctgcc ttttatcggc gcactggtcg tttcgttttc gccacaacgt
    70381 cgggccgccg aatggggggt tttgttcgcc gcgctgacca cgctgtgcat gttgtcgctg
    70441 atctccgcgt tttatcaggc cgataaagtt gccgtcacgt tgacgttggt caacgtgggg
    70501 gatgtggcat tatttggcct ggtcattgat cgcgtgagta cgctgattct gtttgtggtg
    70561 gtgtttctcg gtttgctggt cacgatctac tccacgggtt atctgacgga taaaaatcgc
    70621 gaacacccgc ataacggcac gaatcgttat tacgcatttt tgctggtgtt tatcggcgcg
    70681 atggcgggac tggtactctc ctcgacgctg ctcggtcagt tgttgttttt tgaaattacg
    70741 ggcggctgct cctgggcgtt gatcagttat taccagagcg ataaagcgca gcgttcagca
    70801 ctaaaagcgt tgcttatcac ccatatcggt tcgttggggt tgtatcttgc cgccgccacg
    70861 ctgtttttgc agaccggaac gtttgtgctt agcgcgatga gcgagttaca tggcgacgca
    70921 cgttatctgg tttatggcgg catcctgttt gccgcgtggg ggaaatcggc ccagctaccg
    70981 atgcaagcgt ggctaccgga cgcaatggaa gcgccaacac cgatcagcgc ctatctccac
    71041 gccgcatcga tggtgaaagt gggcgtttac atttttgccc gcgccattat cgacggcggc
    71101 aatatcccgc atgtgattgg cggcgttggc atggtcatga cactggtcac cattctttat
    71161 ggctttctga tgtatttgcc acagcaggat atgaagcggt tgctggcctg gtcgaccatc
    71221 actcaacttg gctggatgtt cttcggcttg tcgctctcca tcttcggctc gcggctggcg
    71281 ctggagggta gcatcgccta catcgtcaac cacgcgttcg ctaaaagcct gtttttcctt
    71341 gtagcaggtg cgctgagtta cagctgcggc acgcgcttgt tgccgcgtct gcgtggcgta
    71401 ttgcacaccc tgccgttgcc aggcgtgggt ttctgcgtgg cagcgctggc aattaccggt
    71461 gtgccgccgt tcaacggctt cttcagtaaa ttcccgctgt ttgccgccgg ttttgcgttg
    71521 tcagtggagt actggatcct gctgcccgcc atgattctgc tgatgattga atcggtcgcc
    71581 agtttcgcct ggtttattcg ctggtttggt cgcgtcgtgc cgggcaaacc gagcgaggcc
    71641 gtcgccgatg ccgcaccgct gccagggtca atgcgcctgg tgttgattgt actgattgtg
    71701 atgtcgctga tttccagcgt aatcgccgcg acctggttgc agtaaggaga tgatgaatga
    71761 ccggttctat gatcgtaaat aatctggcgg gactgatgat gctgacatcg ctgtttgtga
    71821 ttagcgtcaa aagctatcgc ctgtcatgcg gattttacgc ctgccagtcg ctggtgctgg
    71881 tgtctatttt cgccactctc tcgtgcctgt tcgccacaga gcaactgctg atctggtccg
    71941 ccagcgcctt tatcaccaaa gtgctgctgg taccgttaat catgacttac gctgcacgaa
    72001 atattcccca gaacatcccg gaaaaagcgt tattcggtcc ggcaatgatg gcactgctcg
    72061 cggcgttaat tgtcctgctt tgcgcatttg tcgttcagcc cgtgaagcta ccgatggcta
    72121 ccgggctgaa accggcgctg gcggtagcgt taggtcattt tctgcttggc ctgctgtgca
    72181 ttgtcagcca acgcaatatc ctgcggcaaa tttttggtta ctgcctgatg gaaaacggct
    72241 cccatctggt gctggcgctt cttgcctggc gagcaccgga actggtggaa ataggtatcg
    72301 ctaccgacgc catcttcgcc gtcattgtga tggtgttact ggcaagaaaa atatggcgta
    72361 cccacggcac gctggacgtg aacaacttga ccgcgctgaa gggataatga gatgagttat
    72421 tctgtgatgt tcgctttact cctgctcacg ccgctgcttt tttcgctgct ctgttttgcc
    72481 tgccggaaac gggggctctc tgcgacttgt acggtgactg tattacatag cttagggatc
    72541 acactgctgc tgattctagc actctgggtg gtccaaactg ccgctgatgc aggagaaata
    72601 ttcgctgcgg gactgtggct tcatattgat ggtctgggcg gtttgttcct cgccattctt
    72661 ggtgtgattg gctttctcac cggtgtttac tcgattggct acatgcgtca tgaagtggca
    72721 cacggcgagc tttcacccgt tacactgtgc gattactacg gtttcttcca tctgtttttg
    72781 ttcaccatgc tgctggttgt taccagcaat aacctgattg tgatgtgggc ggcgatcgaa
    72841 gccaccacct taagctcggc gtttctggta ggcatttacg gtcagcgttc atcgctggaa
    72901 tcagcatgga agtacatcat tatttgtact gttggtgtcg cttttggtct gttcggtacc
    72961 gtgctggtat acgccaacgc cgccagcgtt atgccgcagg cagaaatggc gatattctgg
    73021 agcgaggttc ttaagcaatc gtccttgctt gacccaacat taatgctgtt ggcctttgtg
    73081 tttgtgctaa ttggctttgg taccaaaacc gggctatttc ccatgcacgc ctggctgccg
    73141 gatgctcaca gtgaagcgcc gagtccggtc agcgccctgc tctccgccgt attgctgaac
    73201 tgcgcgctgt tggtgctgat tcgctattac atcattattt gccaggccat cggcagcgat
    73261 ttccccaacc ggttgttgct catcttcggc atgttgtcgg ttgccgtggc ggcatttttc
    73321 attctggtac agcgggacat taagcgtctg ctggcgtact ccagcgtgga gaacatgggg
    73381 ctggtcgcgg tggcgctagg cattggcggg ccgctgggaa tttttgccgc gctgctgcac
    73441 accttaaacc acagtctggc aaaaacgctg ctgttctgcg gttccggcaa tgtactgctc
    73501 aagtacggca cgcgcgatct caacgtcgtc tgtgggatgc tcaaaatcat gccatttacc
    73561 gccgtgctgt ttggcggcgg tgcgctggcg ctggcgctgg cagggatgcc gcccttcaac
    73621 atttttctta gcgaatttat gaccgttacc gcaggactgg cacgtaatca cctgctgatt
    73681 atcgtcctgc tgttattgct gttaacgctg gtgctggcgg gcctggtacg gatggctgcg
    73741 cgggtgttaa tggcgaaacc gccgcaggcc gttaaccggg gtgatctcgg ctggttgacc
    73801 acctcgccaa tggtgattct gctggtcatg atgctggcga tgggaacgca tattccacaa
    73861 cctgtcatca ggatcctggc gggcgcttcc actatagtcc tctcagggac gcacgacctg
    73921 cctgcacaac gtagcacctg gcatgatttt ttgccttcag gcaccgcatc tgtttcggag
    73981 aaacacagtg aacgttaatt catcgtcaaa tcgtggcgaa gcgattctcg ccgccctgaa
    74041 aacgcagttc cccggcgcgg tgctggatga agagcgacaa acgcctgaac aggtcaccat
    74101 tacggtgaaa atcaatctgc tgcctgacgt tgtacattat ctttattatc aacatgatgg
    74161 ctggcttcca gtcctgtttg gcaacgacga gcggacactt aacggtcatt acgcggttta
    74221 ttatgccctt tcaatggaag gggccgaaaa atgctggatc gtggtgaagg cactggtcga
    74281 tgccgacagt cgggagtttc cgtcagtcac accgcgcgtc cctgccgcgg tctggggcga
    74341 gcgagaaatt cgcgatatgt acgggctgat tccggttggc ctgccggatc agcgtcgcct
    74401 ggtgttgccc gatgactggc cggaagatat gcatccgctg cgcaaagatg cgatggatta
    74461 tcgactgcgc cctgaaccga cgacagattc cgaaacgtat gcgtttatca atgagggcaa
    74521 cagcgatgcg cgggtgatcc ctgtcggccc gctgcatatc acctcctatg aaccgggtca
    74581 cttccgcttg tttgtggatg gcgagcaaat tgtcgatgct gattaccgcc tgttttatgt
    74641 ccatcgcggc atggagaaac tggcagaaac gcggatgggc tacaacgaag tgaccttctt
    74701 atcggaccgc gtgtgtggga tttgcggttt tgcccacagt gtggcctata ccaactcggt
    74761 tgaaaatgca ctggggattg aggtgccgca acgagcacat actattcgct cgattctgct
    74821 ggaagtcgag cggctacaca gtcatttgct caaccttggc ctctcctgcc attttgttgg
    74881 ttttgatacc ggctttatgc aatttttccg cgtgcgggaa aagtcgatga cgatggcgga
    74941 attgctgacc gggtcgcgta aaacctacgg tctgaatctg attggtggtg ttcgccgcga
    75001 tattctcaaa gagcaacgtc tgcaaacgct gaaactggtg cgcgagatgc gcgccgacgt
    75061 gtcggagctg gtagagatgc tgcttgctac gccgaatatg gaacaacgca ctcagggcat
    75121 tggcattctc gaccgacaaa tcgcccgtga ttatagccct gtagggccgc tgatccgcgc
    75181 agtggttttg cccgtgattt gcgctttgat cacccctacg ccgactacgg caatattcca
    75241 aaaacactgt ttacctttac cggcggcgat gttttctccc gcgtgatggt ccgtgtcaaa
    75301 gagacgtttg attcgctggc aatgctggaa tttgccctcg acaacatgcc ggatacccca
    75361 ctgctgaccg aaggctttag ctataaacct cacgcattcg cgctgggctt tgttgaagcg
    75421 ccacgcggtg aagacgtgca ctggagcatg ctcggtgata accaaaaatt gttccgctgg
    75481 cgctgtcgtg ccgccaccta cgccaactgg ccggtgttgc gttacatgct gcgcggcaat
    75541 accgtttctg acgcaccgct gattatcggt agccttgatc cctgctactc ctgtaccgac
    75601 cgtgtgacgc tggtagatgt gcgcaagcgc cagtcaaaaa ccgtgctgta taaagagatc
    75661 gaacgctacg gcattgatcg taaccgttcg ccgctgaagt aaggacagaa gatgctgaag
    75721 ttactgaaaa ctattatgcg cgccggaacc gcgacggtga aatatccctt cgcgccactg
    75781 gaggtcagcc caggctttcg cgggaaaccg gacctgatgc ccagccaatg tattgcctgc
    75841 ggtgcctgcg cctgtgcttg tccggcaaat gcgctgacta tccagaccga cgaccagcaa
    75901 aattcgcgca cctggcagct ctatctgggg cgttgtattt actgcggacg ttgtgaagaa
    75961 gtgtgcccga ccagagccat ccagcttacc aataactttg aactgaccgt cacccataaa
    76021 gccgatctct atacccacgc gacgttccat ctacaacgtt gcagccgttg cgaacgcccg
    76081 tttgccccgc aaaaaaccgt cgcactggct gctgaattgt tggcacagca acaaaatgct
    76141 ccacaaaacc gcgaaatgtt atgggcgcaa gcgagcgtct gcccggagtg caaacaacgc
    76201 gcaacgctga tcaacgacga tacagatgta ccgctggtgg ctaaggagca gctatgagtc
    76261 cagtgcttac gcaatatgtc agccagccca tcacgctgga cgagcaaacg caaaagatga
    76321 agcggcattt gctacaggat atccgtcgct cggcttacgt ttatcgcatc gattgcggcg
    76381 gctgcaacgc ctgtgaaatc gaaatttttg ctgccatcac ccccgtattc gacgcagaac
    76441 gttttggcat taaggttgtt tcatcaccgc gtcacgccga tattttgtta tttactggcg
    76501 cagtcacccg ggcgatgcgt atgcctgcac ttcgggcgta tgagtctgcc cccgatcata
    76561 aaatctgtgt ttcctacggc gcgtgcggtg tcggcggcgg tattttccac gatctctaca
    76621 gcgtctgggg cggcagcgac accattgtcc ccattgatgt ttggatcccc ggctgcccgc
    76681 caacaccggc cgccaccatt cacggtttcg ccgtggcgct cggtttgctg caacagaaga
    76741 ttcacgcggt ggattatcgc gatcccaccg gggtgactat gcaaccgttg tggccgcaga
    76801 tcccgccatc acagcgtatc gccattgagc gagaagcgcg gcggctggcg ggctatcgtc
    76861 aggggcgaga aatttgcgat cggctcctgc gccatttaag cgacgatcct acaggaaatc
    76921 gggttaacac ctggttgcgc gatgccgacg atccacgtct caatagtatc gttcagcaac
    76981 tctttcgcgt actccggggg ttacatgact gaagagtgcg gggaaattgt tttctggacg
    77041 ctgcgaaaaa agtttgtcgc cagtagcgac gagatgccgg aacacagctc tcaggtaatg
    77101 tattactcgc tggctatcgg tcatcacgtt ggcgtgattg attgtctgaa tgtcgccttc
    77161 cgctgcccac tgacggaata cgaagattgg cttgcactgg tcgaagagga gcaagcccga
    77221 cgtaagatgc tgggggtgat gacttttggt gagattgtta ttgacgccag ccacaccgcc
    77281 ctgttgaccc gggcattcgc gccactggca gatgacgcga cgtctgtgtg gcaagcgcgt
    77341 agcattcaat tcattcatct gttggatgaa attgtgctgg aaccggctat ctatctgatg
    77401 gccagaaaaa ttgcgtgaga aggatttctc attaataagg actgttgatg gctatgtcag
    77461 acgaagcgat gtttgccccg ccgcaaggaa taacaattga agcggtaaac ggaatgctcg
    77521 cggagcggtt agcgcagaaa cacggcaagg cgtctttatt acgcgccttc atcccgctgc
    77581 cgccgccgtt cagcccggta caacttattg aactgcatgt tctcaaaagc aacttctatt
    77641 accgctacca tgatgatggc agcgatgtga cggcaacaac agagtatcag ggcgagatgg
    77701 tcgattattc gcgttacgcc gtccttctcg gcagtagtgg aatggcggag ctacgcttta
    77761 ttcgtaccca cggcagccgt tttactcccc aggattgcac actgtttaac tggctggcac
    77821 gcattatcac cccggttctg caatcatggc tcaatgatga agcacagcag gtggcgctgc
    77881 gtttgctgga gaaagatcgc gatcatcatc gggtactggt ggatatcact aatgcagtgc
    77941 tgtcacatct tgatctcgac gatctgatcg ctgacgtcgc tcgtgagatc catcattttt
    78001 tcggtctggc ttcagtcagt atggtactgg gcgatcatcg aaagaacgag aagttcagcc
    78061 tgtggtgcag cgatctttct gcctcacatt gtgcgtgtct gccacacaat atgcctggcg
    78121 acagtgtatt gctgacacaa acgctacaaa cccgacaacc gaccttgacg caccgtgcag
    78181 atgatctgtt tctctggcaa cgcgacccgt tattactctt acttgcatct aacggctgcg
    78241 aatctgcgct ccttataccg cttacctttg gcaaccatac accgggtgca ttgttgctgg
    78301 cgcatacctc ttccactctc tttagtgagg aaaactgcca gctactacaa cacatagccg
    78361 atcgcatcgc tattgccgtt ggcaatgccg atgcctgccg tagaatgacc gatttgcagg
    78421 aaagtttgca gcaagaaaac caccagctta gcgagcagct cctttcgaat ctgggcgtcg
    78481 gtgacattat ctatcaaagc caggcaatgg aagacctgct ccagcaggta gatattgtgg
    78541 cgaagagcga cagtacggtg ttgatttgcg gtgaaaccgg aaccggcaaa gaggtgatcg
    78601 ccagagcgat ccatcaactt agcccgcgac acgacaagcc gctggtcaaa atcaactgcg
    78661 ctgccatccc cgccagtctt ctggaaagtg agttattcgg tcatgacaaa ggggcgttta
    78721 ctggtgcgat taatacccat cgtggtcgtt ttgaaattgc cgatggcggc acgttgtttc
    78781 tcgatgaaat tggcgatctg ccgttagaac ttcagcctaa actgctgcgc gtattgcagg
    78841 aacgggagat tgagcgtctc ggcgggagta gaacgatccc ggtaaatgtc agagtcattg
    78901 ccgccaccaa ccgtgatttg tggcaaatgg ttgaagatcg ccagtttcgc agcgatctct
    78961 tttatcgcct gaatgtcttc ccactggaat tgcctccgct gcgcgaccgt ccggaagata
    79021 tccctctttt agcaaagcat ttcacgcaaa aaatggcgcg ccatatgaat cgttcaattg
    79081 acgccatccc gaccgaggcg ctacgccagt tgatgtcgtg ggactggccg ggcaacgtgc
    79141 gcgagctgga aaacgtgatt gagcgagcgg tactgctgac tcgcggtaac agtctgaatt
    79201 tacatcttaa tgtccgacaa agccgtttac tgccgacgct aaatgaagat tcagcgcttc
    79261 gcagttcaat ggcgtagttg ctgcacccga cgacgccaga gaatgacgaa gaagaacgtc
    79321 agcgcattgt tcaggtattg cgagaaacca atggcattgt tgccgggccc cgtggcgcgg
    79381 cgacacgatt agggatgaag cgcaccacgc tgctgtcacg aatgcagcgt ctgggaatct
    79441 cggttcgcga ggtgttgtaa tctgcttttg caggagtatg catgagaaac aaactctctt
    79501 tcgacttgca gttgagcgcc agaaaagcgg caatcgctga acggattgcc gcccataaaa
    79561 ttgcccgcag taaagtgtcg gtctttttaa tggcgatgtc cgctggcgtg tttatggcga
    79621 tgtccgctgg cgtgtttatg gcgatcggat ttacttttta cctttccgtt atcgccgatg
    79681 ccccgtcttc ataggcatta acccatctgg tgggcggcct ttgctttaca cttggcttta
    79741 ttttgctggc ggtttgcggc accagcctgt tcacctcgtc ggtaatgacg gtaatggcaa
    79801 aaagtcgggg cgttattagt tggcgaactt ggctgattaa cgcacttctg gtggcctgcg
    79861 gtaatctggc aggtattgcc tgtttcagtt tgttaatctg gttttccggg ctggtgatga
    79921 gtgaaaacgc gatgtgggga gtcgcgattt tacactgcgc cgagggcaaa atgcatcata
    79981 catttactga atctgtcagc ctcggcatta tgtgcaatct gatggtttgc ctggcgctgt
    80041 ggatgagtta ttgcggacgt tcgttatgcg acaaaatcgt cgccatgatt ttgtccatca
    80101 ccctgtttgt cgccagtggc tttgagcact gtatcgccaa tttgtttgtg attccgttcg
    80161 ccattgccat tcgccatttc gcccctacct ccttctggca actggcgcac agtagcgcag
    80221 acaattttcc ggcactgacg gtcagccatt ttattaccgc caatctgctc ccggtgatgc
    80281 tgggtaatat tatcggcggt gcagtgctgg tgagtatttg ttatcgggct atttatttac
    80341 gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt cgggctattt atttacgtca
    80401 ggaatcctga aaaatcagcc cggcgaaaca gttcgtcggg ctgaagatta ttcttgcgcg
    80461 atttgcccat cgggccaggc gtggatcacg gctttgatca gcgtcgccaa tggaatggcg
    80521 aagaatacgc cccagaatcc ccacaaacca ccgaagatca ccaccgataa aataatcacc
    80581 agcggatgca ggttaaccgc ttcggagaac aacaccggta ctagcaggtt gccgtccagc
    80641 gcctgaataa tcagatacac cgcgaagcag ctccagaatt cagtacctgc gccaaactgg
    80701 aatagcgcca cgccaaccac cggaatggtc accacaaatg cgccaatgta cggaataaga
    80761 accgagaagc cgaccagcac tgccagcagc agcgaatagt tcagcccgaa gagcaagaac
    80821 cccagccagg tggcgatccc caccacgatc atctccagca ctttgccgcg gatatagttg
    80881 gtgatttgtt gattcatctc cttccacacc tgtcctgcca gcccacggtt acgcggcagc
    80941 acccggcgaa cggcgttcag catctgctct ttgtctttca gcaggaagaa gaccatcaat
    81001 ggcaccagca ccagatagac ggctatggtc agcaaaccga ccagcgaggc gagggaaatt
    81061 ttcaccaccg aatcgcccat ggtcagcatc cgactgcgca tattttcggc cattgcatca
    81121 ataatgcccg cgtccattaa caccggatag cggcgcggca acgtggcggc aaagtcagaa
    81181 agcttattga gcatccccgg catatcgcgg attaagtaga tgccctgttg ccaggcgata
    81241 ggcagtacca cgaacgccat cagtagcaat ataccgacaa aaaccaccaa tacaatcgac
    81301 gtcgcccagc ggcgggagca gccaatagat tgcaggcgca cggttggcca ttccagcaaa
    81361 taagccagca caatagccac cagcaacgga gcaagcaggc cactaaagaa aaagataatg
    81421 ccaaatccgg caactaaaat aaccagcaag gcaatcgctt ccgggtcgct aaaacggcgg
    81481 cgataccatt gcatcaacat ttcgagcata aagaccttcc ctgaacctca agagcgggat
    81541 tgcgatccgc aattgtatcg aaatgtcaca aaaaagactt cgctttttat gacggattca
    81601 ggaaactgaa aagtcatttg agtgggctaa tcttcgccgt tacactcaaa ggcggcgagg
    81661 tgggaacgat atttcacagt atcggtcaaa tgactatctt cagaaataca ggatagaggt
    81721 tatgttcagg cagttgaaaa aaaacctggt tgcaaccctc attgctgcta tgaccattgg
    81781 tcaggtagcc ccggcgtttg ccgacagcgc agacaccttg ccggatatgg gaacctccgc
    81841 aggaagcacg ctttccattg gtcaggaaat gcagatgggc gactattatg tccgccagct
    81901 acgcggcagc gcgccgttaa ttaatgaccc gctgttaacg caatatatta attcgctggg
    81961 gatgcgtctg gtttcgcatg ccaattcggt taagacaccg tttcattttt ttctgatcaa
    82021 caacgacgaa attaacgcct ttgctttctt tggcggcaac gtggtgctgc actctgccct
    82081 gttccgttat tccgataacg aaagtcaact ggcttcagtt atggcgcacg aaatctccca
    82141 cgtcacccaa cgtcacctgg cgcgagcgat ggaagatcaa caacgcagcg cgccgctgac
    82201 ctgggtcggc gcgttaggtt ctattttact ggcgatggcc agtccgcagg cggggatggc
    82261 ggcgctgacc ggtacactgg cgggaacgcg ccaggggatg atcagtttca cccagcaaaa
    82321 tgaacaggaa gcggaccgca ttggtattca ggtgctgcaa cgctcgggat tcgatccaca
    82381 ggcgatgccg accttcctgg aaaaattgct cgatcaggcg cgttactcct cgcgcccgcc
    82441 ggaaatttta ttgactcacc cgttgccgga aagtcgtctg gcagatgccc gcaaccgtgc
    82501 taatcagatg cgcccgatgg tggtgcagtc gtcggaagat ttctatctgg cgaaagcgcg
    82561 cacactgggg atgtataatt ccggacgtaa ccagctcacc agtgatttgc tggatgaatg
    82621 ggcgaaagga aacgttcgtc agcaacgagc ggcgcaatat ggtcgtgctt tacaggcgat
    82681 ggaagccaat aaatacgacg aggcgcgtaa aacgctgcaa ccgttactgg cggcagaacc
    82741 tggcaacgca tggtatctcg atctggctac cgatatcgat cttgggcaaa acaaagccaa
    82801 tgaggcaatc aatcgcctga aaaatgcccg cgatttacgc accaatccgg tgttgcagct
    82861 caacctggcg aacgcttatc tgcaaggcgg tcaaccacaa gaagcggcca atattctgaa
    82921 tcgctacacc tttaataata aagatgacag caacggctgg gatttgctgg cacaggcgga
    82981 agccgcgcta aataaccgcg atcaggagct ggctgcgcga gcagaaggtt atgcgctcgc
    83041 cggacgactc gatcaggcca tttcgctgtt gagtagcgcc agttcgcagg tgaaattagg
    83101 cagcctgcaa caagcgcgtt acgatgcgcg catcgaccag ttgcgccagc tgcagaaacg
    83161 ctttaagcct tataccaaga tgtaattcgc attaaaggaa gaacaatgac caaacaggta
    83221 aaaatctacc ataacccacg ctgttcaaag agccgggaaa cgctgaacct gctgaaagaa
    83281 aacggcgtgg agccagaagt ggtgctctac cttgagacac ccgccgatgc ggcaacgctg
    83341 cgcgatttgc tgaaaatgct ggggatgaat agcgcccgtg aattgatgcg ccagaaagag
    83401 gatctttata aagaactaaa cctggctgac agttcgctta gcgaagaggc gctgattcag
    83461 gcaatggtgg ataatccgaa gctgatggag cgcccgatta tggtcgcgaa tggcaaagcg
    83521 cggattggtc gtccaccgga gcaggtactg gaaatcgtgg gttaagatgt gctccatcgc
    83581 tagttgaagc acattgccgg atgcgacgct tgccacgtct tatccggcct acgaatacta
    83641 tggattaact tcgcgtaggt caggcgttcg cgccgcatcc gacaataaac accttatcta
    83701 caacttcaga atttctttca caaacggaat ggtcagctta cgttgcgcgg taatcgacgc
    83761 acgatccaac tgatccaacg tcataaatag cgtgcgcatt tctctgtcga gccgcttcag
    83821 caagaaacgc cccacatctt ccggcagttc aaaaccacgc aaacgcgcgc gtaactgtag
    83881 cgcctgcaac ttatcttcat cagaaagtgg ctgcaatttg tagatctgcc cccagtcgag
    83941 acgcgacgcg agatccggta atcccagatt caactgccgc ggtggacgat cgccggtgat
    84001 caacaaccgt gttttgcccg attccagaat tcgattgtag agatcgaaaa tcgccatctc
    84061 ccacaactca tcgcctgcaa tacactcaat gttgtcgata cagaccagcg acaaatgctc
    84121 cataccgtcg agcacttccg gaacaaacca ggtgcgttta tccagcggga catagcccac
    84181 cgcatcgcca cgctgcgaca attccgcgca agccgcgtgc agcagatggc tgcgccccgc
    84241 gccttcgcgt gcccagagat agatgtaacc gctatgttcc tgacgcagca cgttttgcag
    84301 cgcggccagt aaagaggagt tatcccccgg ccagaaactt gcaaaggttt cgtcgtcagg
    84361 aagataaagt ggcaaagaga gctgtgccgg tgtgttcaga gatacctcaa ccaggatttc
    84421 acaaaatcgc gagaagttta ccacagaatc ccatgatgtt tgaaccgggc agcaacactg
    84481 cccggtcggt acattatttg tctgttatat ccgcgtcttc tgcgtccagc accacttctt
    84541 cagggcgcag cacactgatc agtttgaaaa tcaaacttaa gccgataccg acgatggtcg
    84601 ccagcgccat accttttaac tccgccgcgc cgatgtttac cttcgcgcca ctgacgccga
    84661 tgatcaaaat cacggaagtc aggatcaggt tctgtgcttt gttgtaatcc actttcgatt
    84721 cgatcaaaac acgaataccg gaagcaccaa tgacaccata aagcagcagc gaaacgccgc
    84781 ccatcaccgg caatgggatc atctggatag cggcagccag tttaccgacg caggaaagca
    84841 ggatagcgaa aatcgccgcc ccgccgataa cccaggtact gtaaacacgg gtgatcgcca
    84901 tcacgccaat gttttctccg taagtggtat ttggcgtaga gccaaagaag ccggagatca
    84961 cggtcgacag gccgttagca aacatcgaac ggtgcagacc tggatcgcgc agcagatctt
    85021 ttttgacgat attagccgtt actaccaggt gccctacgtg ttcggcaata accactaacg
    85081 ccgctggcag aatagtcaga atggcaaacc actcgaagcg cggcgtatag agggttggca
    85141 gtgcaaacca gtgagcatta ataatcggcg tggtatcgac aattcccatt gcgaaagaga
    85201 gcgcgtaccc caccagcacg ccaattaaaa tcgggataat tgccaggaaa ccacgaaaca
    85261 gcacggaacc taaaaccgtg accgccaggg tggtaataga gatgatgatg gttttggagt
    85321 ttggcgtttg cccttcagcc gggagtaaac ccgccatacc ggcagctacg cccgccagct
    85381 ccagaccgat gacggcaacg attgcgccca ttgccgcagg tggaaacagc acgtccaacc
    85441 agccggtccc cgctttcttc acgataaaag aaaccaggca gaacagcacg ccgcacataa
    85501 taaagccgcc cagcgcgact tcatacccta acggcaacag taacaatacc ggtgaaataa
    85561 aggcaaagct ggaaccaaga taagccggaa ttttcccttt acagatgaag agatacagca
    85621 gcgttccaat accgttaaat aacagtacag tcgccgggtt aatatgaaat aagacgggca
    85681 ccaggacggt tgcaccaaac atggcgaaca aatgttgcaa actaagcggg attgtctgta
    85741 aaagtggcgg tctttcactc accccgatag cacggcgcgt catagtatta tcctctgtat
    85801 tatgtgttat aggcgcttta ctcaaaaaaa agccgactct taaagtcggc tttaattatt
    85861 tttattcttt atttcgtacc aaagattttg tcaccggcat cgccgaggcc cggaataatg
    85921 tatccgtgct cgttcagtcc ctggtcaatc gatgcagtat acagttcgac gtccgggtgc
    85981 gctttttcca gcgcagcgat accttctggc gcagcgacca gcaccagaac tttgatgctg
    86041 ctgcagcccg cttttttcag caggtcgatg gtcgcgataa cggaaccacc ggttgccagc
    86101 attgggtcaa cgatcagcgc catacgctca tcgatgttag aaaccagttt ctggaagtac
    86161 ggtaccggct ccagcgtttc ttcattacgg tacataccga caacgctgat gcgcgcgctc
    86221 ggaacgtttt ccagcacacc gtccatcata ccaagacccg cacgcagaat tggcacaacg
    86281 gtaattttct tacctttgat ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt
    86341 actttttccg tttcgaggtc ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa
    86401 gcgagttcgc gaaagcgctt ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt
    86461 ttgacgagtg ggtgtttgac ttccacgatc ttcatactct ttctcctttg aggggcagcc
    86521 acaaaaaaat cgacggatta tacctccttt cttcagggcg gcaatattat tttcgttgac
    86581 tttagtcaaa atgataacag tttgagataa agttatttta tattcaaatg gttatgaaag
    86641 aagattattc catccgaaaa ctaacctttg ccctggcaca agtcttcttt cgccgcgcgc
    86701 ccggggaaaa gacgtgcaaa aaggttgtgt aaaacagtct cgcaaacgtt tgctttccct
    86761 gttagaattg cgccgaattt tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt
    86821 gaccgataaa acctctctta gctacaaaga tgccggtgtt gatattgacg cgggtaatgc
    86881 tctggttgga agaatcaaag gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg
    86941 tctgggcggc ttcggtgcgc tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt
    87001 ttccggcact gacggcgtag gtaccaagct gcgtctggca atggacttaa aacgtcacga
    87061 caccattggt attgatctgg tcgccatgtg cgttaatgac ctggtggtgc aaggtgcgga
    87121 accgctgttt ttcctcgact attacgcaac cggaaaactg gatgttgata ccgcttcagc
    87181 ggtgatcagc ggcatcgcgg aaggttgtct gcaatcgggc tgttctctgg tgggtggcga
    87241 aacggcagaa atgccgggga tgtatcacgg tgaagattac gatgtcgcgg gtttctgcgt
    87301 gggcgtggta gaaaaatcag aaatcatcga cggctctaaa gtcagcgacg gcgatgtgct
    87361 gattgcactc ggttccagcg gtccgcactc gaacggctat tcgctggtgc gcaaaattct
    87421 tgaagtcagc ggttgtgatc cgcaaaccac cgaacttgat ggtaagccat tagccgatca
    87481 tctgctggca ccgacccgca tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga
    87541 tgtgcatgcc attgcgcacc tgaccggcgg cggcttctgg gaaaacattc cgcgcgtatt
    87601 gccagataat acccaggcag tgattgatga atcttcctgg cagtggccgg aagtgttcaa
    87661 ctggctgcaa acggcaggta acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg
    87721 cgtcgggatg attatcgccc tgcctgctcc ggaagtggac aaagccctcg ccctgctaaa
    87781 tgccaacggt gaaaacgcgt ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg
    87841 cgtggttatc gaataatgaa tattgtggtg cttatttccg gcaacggaag taatttacag
    87901 gcaattattg acgcctgtaa aaccaacaaa attaaaggca ccgtacgggc agttttcagc
    87961 aataaggccg acgcgttcgg ccttgaacgc gcccgccagg cgggtattgc aacgcatacg
    88021 ctcatcgcca gcgcgtttga cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc
    88081 gacatgtacg cacccgatgt ggtcgtgctg gctggtttta tgcgcattct cagcccggcg
    88141 tttgtctccc actatgccgg gcgtttgctg aacattcacc cttctctgct gccgaaatat
    88201 cccggattac acacccatcg tcaggcgctg gaaaatggcg atgaagagca cggtacatcg
    88261 gtgcatttcg tcaccgatga actggacggt ggcccggtta ttttacaggc gaaagtcccg
    88321 gtatttgctg gtgatacgga agatgacatc accgcccgcg tgcaaaccca ggaacacgct
    88381 atttatccac tggtgattag ctggtttgcc gatggtcgtc tgaaaatgca cgaaaacgcc
    88441 gcgtggctgg atggtcaacg tctgccgccg cagggctacg ctgccgacga gtaatgcccc
    88501 cgtagttaaa gcgccagctc tgccgctggc gtttttcaat tcacctgtaa atcgcaagct
    88561 ccagcagttt ttttccccct tttctggcat agttggacat ctgccaatat tgctcgccat
    88621 aatatccagg cagtgtcccg tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag
    88681 ctatacatcg aaaaagagct cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg
    88741 gcggacaaat ctaacccgct gattgaaagg atgcgtttcc tggggatcta ttccaataac
    88801 cttgatgagt tctataaagt ccgcttcgct gaactgaagc gacgcatcat tattagcgaa
    88861 gaacaaggct ccaacgctca ttcccgccat ttactgggca aaattcagtc ccgggtgctg
    88921 aaagccgatc aggaattcga cggcctctac aacgagctat tgctggagat ggcgcgcaac
    88981 cagatcttcc tgattaatga acgccagctc tccgtcaatc aacaaaactg gctgcgtcat
    89041 tattttaagc agtatctgcg tcagcacatt acgccgattt taatcaatcc tgacactgac
    89101 ttagtgcagt tcctgaaaga tgattacacc tatctggcgg tggaaattat ccgtagcgat
    89161 agcatccgtt acgcgctgct ggagatccca tcagataaag tgccgcgctt tgtgaattta
    89221 ccgccagaag cgccgcgtcg acgcaagccg atgattcttc tggataacat tctgcgttac
    89281 tgccttgatg atattttcaa aggcttcttt gattatgacg cgctgaatgc ctattcaatg
    89341 aagatgaccc gcgatgccga atacgattta gtgcatgaga tggaagccag cctgatggag
    89401 ttgatgtctt ccagtctcaa gcagcgttta actgctgagc cggtgcgttt tgtttatcag
    89461 cgcgatatgc ccaatgcgct ggttgaagtg ttacgcgaaa aactgactat ttcccgctac
    89521 gactccatcg tccccggcgg tcgttatcat aattttaaag actttattaa tttccccaat
    89581 gtcggcaaag ccaatctggt gaacaaacca ctgccgcgtt tacgccatat ttggtttgat
    89641 aaagcccagt tccgcaatgg ttttgatgcc attcgcgaac gcgatgtgtt gctctattat
    89701 ccttatcaca cctttgagca tgtgctggaa ctgctgcgtc aggcttcgtt cgacccaagc
    89761 gtactggcga ttaaaattaa catttaccgc gtggcgaaag attcacgcat catcgactcg
    89821 atgatccacg ccgcacataa cggtaagaaa gtcactgtgg tggttgagtt acaggcgcgt
    89881 ttcgacgaag aagccaacat tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt
    89941 atcttctctg cgccggggct gaaaattcac gccaaactgt tcctgatttc acgtaaagaa
    90001 aacggtgaag tggtccgtta cgcacacatc gggaccggga actttaacga aaaaaccgcg
    90061 cgtctttata ctgactattc gttgctgacc gcagatgcgc gtatcaccaa cgaagtacgg
    90121 cgggtattta actttattga aaacccatac cgcccggtga catttgatta tttaatggtg
    90181 tcaccgcaaa actctcgccg tctgttatat gaaatggtag accgcgaaat cgccaacgcg
    90241 cagcaagggc tgcccagtgg tatcaccctg aagctaaata accttgtcga taaaggcctg
    90301 gttgatcgtc tgtatgcggc ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga
    90361 atgtgttcgc tgatccccaa tctggaaggc attagcgaca acattcgtgc catcagtatt
    90421 gttgaccgtt accttgaaca tgaccgggtt tatatttttg aaaatggcgg cgataaaaag
    90481 gtctaccttt cttccgccga ctggatgacg cgcaatattg attatcgtat tgaagtggcg
    90541 acaccgctgc tcgatccgcg cctgaagcag cgggtgctgg acatcatcga catattgttc
    90601 agcgatacgg tcaaagcacg ttatatcgat aaagaactca gtaatcgcta cgttccccgc
    90661 ggcaatcgcc gcaaagtacg ggcgcagttg gcgatttacg actacatcaa atcactcgaa
    90721 caacctgaat aaccctatgc caatacacga taaatcccct cgtccgcagg agtttgctgc
    90781 ggtcgatctt ggttcaaaca gttttcacat ggtcatagcc cgtgtggtag atggtgccat
    90841 gcagattatt ggccgcctga aacagcgggt gcatctggcg gacggcctgg ggccagataa
    90901 tatgttgagt gaagaggcaa tgacgcgcgg tttgaactgt ctgtcgctgt ttgccgaacg
    90961 gctacaaggg ttttctcctg ccagcgtctg tatagtcggc acccatacgc tgcgtcaggc
    91021 gctgaacgcc actgactttc tgaaacgcgc ggaaaaggtc attccctacc cgattgaaat
    91081 tatttccggt aatgaagaag cccgtctgat ttttatgggc gtggaacata cccaaccgga
    91141 aaaaggtcgc aaactggtta ttgatattgg cggcggatct acggaactgg tgattggtga
    91201 aaatttcgaa cctattctcg ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct
    91261 ttattttcct ggcggggtca tcaataaaga gaattttcag cgcgctcgca tggcggcagc
    91321 acaaaaactg gaaactttaa cctggcaatt ccgtattcag ggctggaacg ttgcaatggg
    91381 cgcttccggt accataaaag ccgcccatga agtgttaatg gaaatgggcg agaaagacgg
    91441 gataattacc ccggaacgtc tggaaaaact ggtaaaagaa attttacgtc accgtaattt
    91501 cgcatcgctg agtttaccgg gtctttccga agagcggaaa acagtcttcg ttcctggact
    91561 ggcgatttta tgtggtgtgt ttgatgcttt agccatccgt gaactgcgcc tttctgacgg
    91621 ggcgttacgc gaaggcgtac tgtatgaaat ggaaggacgt ttccgtcatc aggatgtgcg
    91681 tagtcgcacc gccagcagcc tcgccaacca gtatcacata gacagcgaac aggcccgacg
    91741 agtgctggat accactatgc aaatgtacga acagtggcgg gaacagcaac cgaagctggc
    91801 gcatccgcaa ctggaggcgc tactgcgatg ggccgccatg ctgcatgagg tcgggttgaa
    91861 tatcaaccac agcggtttgc atcgccactc cgcttatatt ctgcaaaaca gtgacttgcc
    91921 gggttttaat caggaacagc agctgatgat ggcgacactg gtgcgctatc accgtaaagc
    91981 gattaagctc gacgatctgc cgcgctttac cttgtttaag aagaaacagt tcctgccact
    92041 gatacagcta ttgcgccttg gcgtattact aaacaatcaa cgtcaggcaa ccaccacacc
    92101 gccaacattg acactgatta ctgatgacag tcactggaca ctgcgtttcc cgcatgactg
    92161 gtttagtcag aatgcgctgg tactgcttga tctggaaaag gagcaagaat actgggaagg
    92221 cgtggctggc tggcggttga aaattgaaga agaaagtacc ccagaaatcg ccgcttaatg
    92281 ctttgcgggc cgacgagaaa tgtcggcccg cattattcag gcactttcgc gaatgggttc
    92341 gatttcattc agcgtatcaa ttaacggttg cggcttacca ataagataac cctgcatata
    92401 atcgatcccc aaagagagca ccgcctcgcg gatctcttcg ttttcaacgt actctgccac
    92461 tacccgcatt ttcttcattc gcgccagatg gcaaatcgac gccacaatct gataatccag
    92521 actatttgac acaatattgc ggataaagct gccgtcaatt ttaagcagat cggcattcac
    92581 atttttaagc cgtgcatagc tggcgtagcc ggtgccgaaa tcatcaatcg caatctggca
    92641 gcctaattcc tgaagatgct gcaaggtgat ttgcgcctgc ttaacattgg tcagagcatt
    92701 actttcggtg acttcaaaaa taagttgcca cgcttcaatc tgatatttag ccagcaactg
    92761 actgacttca acaggaaaac gagcctgaca taccgaggtt ggagacagat taatagcaaa
    92821 acggtgagcg ggcatcttcg ctctgttttc agccataaat tgcagcgtat gctcaatgac
    92881 ccacatgtcg atactcgacg ataaaccaaa ttcgtgcgcg accggcaaga agctaccggg
    92941 gccgatcagt tcatcattct cacctttcat gcgcagaaga atttcatggt aaacatcacc
    93001 acgcataccg gtaatcggct gggccatcag gaaaaaatgg ttgtgttcca gcgcctgctg
    93061 tagtcgattc atcatcgcga ctttatcttt caattcgcgt tgcaaataca ttgccccgca
    93121 acgctgcata ttttccgggg cgttggtcac gatggaaagt tcggcgaccg tatttagctc
    93181 tcccagcagc aggtagatat gattcactgg cgagcgcaca tagcagtaac tgacgccaat
    93241 ctgcggttgc atcggcatcc catcccaaaa gaaacgaaat tgcttgagat gactatccag
    93301 tgcggtaatg cgctcctggt gcgattctgt attcagtcgc agcgcgagat cgttacccga
    93361 aagctgataa acatcttcac ccggttccag caagggtgac agccagtgag aaa
//
LOCUS       DAJTRN010000003        72970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRN010000003 DAJTRN010000000
VERSION     DAJTRN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO328_000197"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO328_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5928899.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO328_000198"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO328_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5928900.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO328_000199"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO328_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5928901.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO328_000200"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO328_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5928902.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO328_000201"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO328_000201"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5928903.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO328_000202"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO328_000202"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5928904.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO328_000203"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO328_000203"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5928905.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO328_000204"
     CDS             complement(4598..5320)
                     /locus_tag="OO328_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5928906.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO328_000205"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO328_000205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5928907.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO328_000206"
     CDS             6520..6738
                     /locus_tag="OO328_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5928908.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO328_000207"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO328_000207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5928909.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO328_000208"
     CDS             complement(7472..7672)
                     /locus_tag="OO328_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5928910.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO328_000209"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO328_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO328_000210"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO328_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5928911.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO328_000211"
     CDS             10101..12014
                     /locus_tag="OO328_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5928912.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO328_000212"
     CDS             12014..13036
                     /locus_tag="OO328_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5928913.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO328_000213"
     CDS             13030..13248
                     /locus_tag="OO328_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5928914.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO328_000214"
     CDS             13302..14171
                     /locus_tag="OO328_000214"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5928915.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO328_000215"
     CDS             complement(14226..14630)
                     /locus_tag="OO328_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5928916.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO328_000216"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO328_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5928917.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO328_000217"
     CDS             15615..17705
                     /locus_tag="OO328_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5928918.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO328_000218"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO328_000218"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5928919.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO328_000219"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO328_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5928920.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO328_000220"
     CDS             complement(19673..20275)
                     /locus_tag="OO328_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5928921.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO328_000221"
     CDS             complement(20265..20432)
                     /locus_tag="OO328_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5928922.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO328_000222"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO328_000222"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5928923.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO328_000223"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO328_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5928924.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO328_000224"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO328_000224"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5928925.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO328_000225"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO328_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5928926.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO328_000226"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO328_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5928927.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO328_000227"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO328_000227"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5928928.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO328_000228"
     CDS             28269..28436
                     /locus_tag="OO328_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5928929.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO328_000229"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO328_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO328_000230"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO328_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5928930.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO328_000231"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO328_000231"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5928931.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO328_000232"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO328_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5928932.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO328_000233"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO328_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5928933.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO328_000234"
     CDS             complement(33764..34849)
                     /locus_tag="OO328_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5928934.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO328_000235"
     CDS             complement(34861..36165)
                     /locus_tag="OO328_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5928935.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO328_000236"
     CDS             complement(36177..36530)
                     /locus_tag="OO328_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5928936.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO328_000237"
     CDS             complement(36541..37419)
                     /locus_tag="OO328_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5928937.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO328_000238"
     CDS             complement(37416..38642)
                     /locus_tag="OO328_000238"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5928938.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO328_000239"
     CDS             complement(38643..39806)
                     /locus_tag="OO328_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5928939.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO328_000240"
     CDS             complement(39890..40252)
                     /locus_tag="OO328_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5928940.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO328_000241"
     CDS             complement(40269..41174)
                     /locus_tag="OO328_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928941.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO328_000242"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO328_000242"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5928942.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO328_000243"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO328_000243"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5928943.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO328_000244"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO328_000244"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5928944.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO328_000245"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO328_000245"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5928945.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO328_000246"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO328_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5928946.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO328_000247"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO328_000247"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5928947.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO328_000248"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO328_000248"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5928948.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="OO328_000249"
     CDS             complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="OO328_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5928949.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="OO328_000250"
                     /pseudo
     CDS             complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="OO328_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="OO328_000251"
     CDS             complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="OO328_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5928950.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50254..50793)
                     /gene="hofN"
                     /locus_tag="OO328_000252"
     CDS             complement(50254..50793)
                     /gene="hofN"
                     /locus_tag="OO328_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5928951.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="OO328_000253"
     CDS             complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="OO328_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5928952.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51692..54244
                     /gene="mrcA"
                     /locus_tag="OO328_000254"
     CDS             51692..54244
                     /gene="mrcA"
                     /locus_tag="OO328_000254"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5928953.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="OO328_000255"
     CDS             complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="OO328_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5928954.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55289..57424
                     /gene="igaA"
                     /locus_tag="OO328_000256"
     CDS             55289..57424
                     /gene="igaA"
                     /locus_tag="OO328_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5928955.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57489..58157
                     /gene="yrfG"
                     /locus_tag="OO328_000257"
     CDS             57489..58157
                     /gene="yrfG"
                     /locus_tag="OO328_000257"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5928956.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58168..58569
                     /gene="hslR"
                     /locus_tag="OO328_000258"
     CDS             58168..58569
                     /gene="hslR"
                     /locus_tag="OO328_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5928957.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58594..59463
                     /gene="hslO"
                     /locus_tag="OO328_000259"
     CDS             58594..59463
                     /gene="hslO"
                     /locus_tag="OO328_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5928958.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59607..61358)
                     /locus_tag="OO328_000260"
     CDS             complement(59607..61358)
                     /locus_tag="OO328_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5928959.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61737..63359
                     /gene="pckA"
                     /locus_tag="OO328_000261"
     CDS             61737..63359
                     /gene="pckA"
                     /locus_tag="OO328_000261"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5928960.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="OO328_000262"
     CDS             complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="OO328_000262"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5928961.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="OO328_000263"
     CDS             complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="OO328_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5928962.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65731..66207
                     /gene="greB"
                     /locus_tag="OO328_000264"
     CDS             65731..66207
                     /gene="greB"
                     /locus_tag="OO328_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5928963.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66304..68625
                     /locus_tag="OO328_000265"
     CDS             66304..68625
                     /locus_tag="OO328_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5928964.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69063..69290
                     /gene="feoA"
                     /locus_tag="OO328_000266"
     CDS             69063..69290
                     /gene="feoA"
                     /locus_tag="OO328_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5928965.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69307..71628
                     /gene="feoB"
                     /locus_tag="OO328_000267"
     CDS             69307..71628
                     /gene="feoB"
                     /locus_tag="OO328_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5928966.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71628..71864
                     /gene="feoC"
                     /locus_tag="OO328_000268"
     CDS             71628..71864
                     /gene="feoC"
                     /locus_tag="OO328_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5928967.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72067..72954
                     /gene="rpnA"
                     /locus_tag="OO328_000269"
     CDS             72067..72954
                     /gene="rpnA"
                     /locus_tag="OO328_000269"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5928968.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17587 a        19776 c        18455 g        17152 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgccgggtaa gattagcgta
    48121 aaaaagacag caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa
    48181 gagcaccaac tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga
    48241 tgaaacacgt tcagcataaa aacattaccg cttttacggc tgtttactca ctggaaacca
    48301 gtcgtggcgt gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa
    48361 ataattgccc gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat
    48421 ttttacgggt aaaaatgccg cccagcgcca acgtttctcc gctttttacc tcgacctgcg
    48481 tttcgatctc ctgcttatca atcgccagca cttcgccatc ggcctgctgt agcacctgcc
    48541 ccggaacgtt ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg
    48601 gcgtgacctc catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc
    48661 cgctggaaac ctgatatgga atttcgctct cctgtttaat gctggcaggc tgaagatgtg
    48721 aggccagcag acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcggaaa
    48781 gctcaagatc cagcaagcgt ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg
    48841 ccgtcgctac ggagaggtcg ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt
    48901 gcgcatcggc cagcgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga
    48961 caatatgcgc cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt
    49021 caagcgcgct taacgccgtt ttgttatctc gtagcaaaag gcgattggtg cgtttatcga
    49081 cggtcatact ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccgcgt
    49141 cggcgtattg cagggttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct
    49201 gctccgcctc ctggcgggcg atattgtcat tctgccaggc aatggaatgc actgagagaa
    49261 tgttgccttc ctgccgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcctgct
    49321 tccagggaac atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacacgacca
    49381 ggttcaactt ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca
    49441 ccatcagcgt cactttttgc ggctttgccg cctgtacgcc gggtatcagc atcaacagta
    49501 gtgcggctat ccattgcttc atttgtatct ccttgccgtt gccacaacca ttgtggcggt
    49561 tcgcagtttg tcccggtacc cagcgttagt acgtctggcg tcagctgtaa aattgtccag
    49621 ccgttttcca gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt
    49681 acaccgatga tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg
    49741 ctaatccggc atatagatct tccggcgatt taaaagggtc acgcataccg gttaaaaggc
    49801 acaatgcaat acctgccaac aaccagcgtt taaccctcat gcggcatctc cagttgtagc
    49861 gtgaacaaaa gatcatcacc ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc
    49921 tctgccagcc gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactca
    49981 cctccctgcg cggatggatg ccaggaaacc agttgcgcgc cggataactg aaaatccagt
    50041 ggcgaaaagg gcagcgtttt ttcctcgctg aagggagtgg tgtctaccag gcgatacagt
    50101 gcggcccact gctggtgatg acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg
    50161 tgtgtcgatg atagaaaaat gagcgtaact aacatcagca accagaatgc ccagcaaaac
    50221 tggcggaggc ggggtgatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt
    50281 ccttgttaac tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggctccccg
    50341 ctgattgaga tgaaaagacg catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt
    50401 aatgcttgtt gtcagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag
    50461 ccaggcgtgc tcgggtaaaa gcgccgccag tgcttccagc gcagattgcc agtcgcgggt
    50521 aaattgtcgc tggcgctgcc gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa
    50581 acgtggcttc gttatctgta agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc
    50641 gtctatgcgc gcttcggcgc tgcctgtcag acgcagtatt agcgttatcc cgacggccag
    50701 cagcagaggc gcaacgaaca tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg
    50761 tcgccagggc aaaaaattaa ttggcggatt catcagtacg ccttcccaag cgccagcccc
    50821 agcgcgatgg caaagtctcc accaggcggc ggtagcggcg gctgacgaac agaaacggcc
    50881 tcccaggggt caaatccgcc ttcgccacat atcgcgacgc tgtcgggatc aacggataac
    50941 gctgccgcca gctccttcgc gctagtcatc cccaccgcca gtttgcgccc ccagctatag
    51001 cgcgtcgccc acagccactg ttcgttatca cgccaggcca gacattgctg atgagaaggt
    51061 aaaaaaggca ggaatcgctg taatgcactg gcatccgggg tgatcgcact aacatgaaca
    51121 cgcaaccttt ctgccagcgt aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg
    51181 ttataagcgg ggctgagtga gtcttcgctg taatcgaagc gcagggagtc cggatccata
    51241 tccagctcgc gggccatcgt ccctgacagc caggccgttt gctcccgctc accaagggac
    51301 atcgacgggc gcggaaatga ccgctgtaat gtgcgactgg cgggaaacgc caacataatg
    51361 tgatgacgct gcggcagttc gcgactccac ggtaacaacg ttttagccag ctgctgcgca
    51421 tcaacaatcc gcccatcttt gataatgtcg tgcgccagcg gcaaccgcca ccagcgttgc
    51481 aaaaagcatt cttttgcgcc ccgcacgatc gcaaccgctg ccgcttcttg ctgttgtaaa
    51541 tgcaaaccaa tttgccagat cttaaatgtc attgtgatga tctccttatc acccgtcact
    51601 ctgacgggta tatcaatgcg tctggcttgc ctttatacta ccgcgcgttt gtttataaac
    51661 tgcccaaatg aaactaaatg ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct
    51721 tgcagtctgt tgcattctgc tgggagcagg ctcgatttat ggcctatacc gctacatcga
    51781 gccacaactg ccggatgtgg tgacattaaa agatgttcgc ctgcaaattc cgatgcagat
    51841 ttacagcgcc gatggcgagc tgattgctca atacggtgag aaacgtcgta ttccggttac
    51901 gttggatcaa atcccaccgg agatggtgaa agcctttatc gcgacggaag acagccgctt
    51961 ctacgagcat cacggcgttg acccggtggg gatcttccgt gcggcaagtg tggcgctgtt
    52021 ctccggccac gcgtcacaag gggcgagtac cattacccag cagctggcga gaaatttctt
    52081 cctcagtcca gaacgcacga tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat
    52141 tgaacagttg ctgacgaaag acgaaatcct cgagctttat ctgaacaaga tttaccttgg
    52201 ttaccgcgcc tatggtgtcg gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca
    52261 actgacgctg aacgaaatgg cggtgatagc cgggttgccg aaagcgcctt ccacctttaa
    52321 tccgctctac tcaatggatc gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct
    52381 ggatgaaggg tatatcaccc agcaacagtt cgatcagaca cgcactgagg cgattaacgc
    52441 taattatcac gcgccggaga ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca
    52501 ggagatgtat aaccgttatg gcgaaagtgc ctatgaagac ggttatcgca tttacaccac
    52561 catcacccgc aaagtgcagc aggccgcgca gcaggcggta cgtaataacg tgctggacta
    52621 cgacatgcgc cacggctatc gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc
    52681 gtgggataac aacaagatta ccgatacgct gaaggcgctg ccgacctatg gtccgctgct
    52741 gcctgccgca gtcaccagcg ccaatcctca gggagcgacg gcgatgctgg cggacgggtc
    52801 gaccgtcgca ttgagtatgg aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca
    52861 gcagggaccg acgccgcgta aagtgaccga tgttctgcaa acgggtcagc aaatctgggt
    52921 tcgtcaggtt ggcgatgcat ggtggctggc acaagtgccg gaagtgaact cggcgctggt
    52981 gtcgatcaat ccgcaaaatg gtgccgttat ggcgctggtc ggtggctttg atttcaatca
    53041 gagcaagttt aaccgcgcca cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt
    53101 cctctacacc gcggcgatgg ataaaggtct gacgctggca agtatgttga acgatgtgcc
    53161 aatttctcgc tgggatgcag gtgccggttc tgactggcag ccgaagaact caccaccgca
    53221 gtatgctggt ccaattcgct tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt
    53281 acgtgcaatg cgggcgatgg gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt
    53341 cccggcgcaa aacattgtcc acaccgaatc gctggcgctg ggttcagcgt ccttcacccc
    53401 aatgcaggtg gcgcgcggct acgcggtcat ggcgaacggc ggcttcctag tggacccgtg
    53461 gtttatcagc aaaattgaaa acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt
    53521 agcctgcccg gaatgcgata ttccagtgat ttacggtgat acgcagaaat cgaacgtgct
    53581 ggaaaataac gatgttgaag atgtcgctat ctcccgcgag cagcagaatg tttctgtacc
    53641 aatgccgcag ctggagcagg caaatcaggc gttagtggcg aagactggcg cgcaggagta
    53701 cgcaccgcac gtcatcaaca ctccgctggc attcctgatt aagagtgctt tgaacaccaa
    53761 tatctttggt gaaccaggct ggcagggtac cggctggcgt gcaggtcgtg atttgcagcg
    53821 tcgcgatatc ggcgggaaaa ccggtaccac taacagttcg aaagatgcgt ggttctcggg
    53881 ttacggtccg ggcgttgtga cctcggtctg gattggcttt gatgatcacc gtcgtaatct
    53941 cggtcataca acggcttccg gagcgattaa agatcagatc tcaggttacg aaggcggtgc
    54001 caagagtgcc cagcctgcat gggacgctta tatgaaagcc gttcttgaag gtgtgccgga
    54061 gcagccgctg acgccgcctc cgggtattgt gacggtgaat atcgatcgca gcaccgggca
    54121 gttagctaat ggtggcaaca gccgcgaaga gtatttcatc gaaggtacgc agccgacaca
    54181 acaggcggtg cacgaggtgg gaacgaccat tatcgataat ggtgaggcac aggaattgtt
    54241 ctgattaaaa aggcgcttcg gcgccttttc agttggctga caaagtgcat ttgtttatgc
    54301 cggatgcggg tgaacgcgtt atccggctaa caaaatcgtg aaaaatcaat atattgcagg
    54361 aaccccataa gcctgataag cgttgtgcat caggcaaact tcacgcattt acactcgccc
    54421 ctgccctttc aaccattcgc gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa
    54481 gtcagggtct tccagcaaat ccatcatatg cgccagcggc cagcgcacct gtggtagcgg
    54541 ctctggctca tcgccttcca gcgattccgg gtagagatct tgcgcgacca cgatattcat
    54601 tttgctggaa aagtaagacg gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc
    54661 accaaatcca acctcttctt ttagctcgcg gttagcggct tcgtagacgc tttcacccgg
    54721 atcaattaat cctttcgaaa aacctaattc gtaggattca gttcccactg cgtattcgcg
    54781 gatcaggatc aggtgatcgt ccacaatcgg cacaatcatc actgcttccc ggttggttgg
    54841 acgcattcgt tcataaacac gccgcacgcc attgctgaac tccagatcca cgctctcgac
    54901 ggtaaacagt cgggaacggg ctacagtttc aacattcaga atggtgggtt tttgtaatga
    54961 tttgttcatc gtgggatcta tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc
    55021 tttcggcaat gtgaattgca tgttatttac atttatgtaa cttaataaat aatcgtcctc
    55081 aaatcaaatt aaaagtcaat aggttgaaat aactccagga atttgctgat attccgcctt
    55141 cagagggttt gctatgatca gcggttactg tgatgtgctt aatgatgctc aagttaagct
    55201 ccacgcttgc cgatagccaa ccgcagaatc atgtattgtg tccggtgcga ctgaccacgc
    55261 ctgacagact aagtaagatg gggaaagcat gagcaccatt gtgatttttt tagctgcttt
    55321 gctggcctgc tcactacttg cgggatggct gataaaagtg cgatccagac ggcgtcagct
    55381 gccctggacc aacgccttcg cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag
    55441 cgccgttgaa aattatcttg agagcctgac gcaggtatta caggtgcctg gcccaacgga
    55501 agctagcgcg gcaccgatct ctctggcgct gaatgccgaa agcaacaacg tcatgatgct
    55561 gacacacgct atcacacgtt acggcatctc taccgacgat ccgaataaat ggcgttacta
    55621 cctcgattcg gtagaagtcc acctgccccc cttctgggaa cagtacatca acgatgagaa
    55681 taccgttgaa ctgattcata ccgattcgct gccgttggtt atttcactca acggtcatac
    55741 gctgcaggag tacatgcagg aaacgcgcgg ctatgccttg caacctgttc cgtccacgca
    55801 ggcgtcgatt cgcggggaag agagtgagca aatcgagcta ctcaatattc gcaaagaaac
    55861 gcatgaagaa tatgcgctga gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc
    55921 ctccttcctg atgttctttt tctgcctgat taccccggat gtatttgttc cgtggctggc
    55981 aggcggcgca ttactgctgc tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa
    56041 atcctccctg cgagaaattc attgtctgcg cggtacaccc cgtcgttggg gattgtttgg
    56101 cgaaaacgat caggaacaga tcaacaatat ttcactcggt attatcgacc tggtctatcc
    56161 cgcacactgg cagccataca ttgctcagga tctcggtcaa caaaccgata ttgatatcta
    56221 tctcgaccgc cacgtagtgc gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa
    56281 ctttccgtta cagcactggc tgcgcagtac gattatcgct gcgggctcgc tgctagtgct
    56341 gtttatgctg ttattctgga tcccgctgga tatgccgctg aaattcactc tctcatggat
    56401 gaaaggcgcg cagaccattg aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg
    56461 ggtaggcgat acattgcgta ttagcggtac gggaatgtgt aatattcgca cttccggaac
    56521 ctggagcgcg aaaaccaatt cacctttttt accgtttgac tgctcgcaga tcatctggaa
    56581 tgacgcccgc tcattaccat taccagaatc tgaactggtc aacaaagcga cggcattgac
    56641 tgaagcagtt aatcgccagc tgcaccctaa accggaagat gaatctcgcg tcagtgcctc
    56701 attacgttca gcgattcaaa aatccggcat ggtattgctt gatgattttg gcgacattgt
    56761 actgaagaca gcggatttat gttctgccaa agatgactgt gtgcgactga aaaatgcgct
    56821 ggtcaatctc ggcaacagta aagactggga cgcgctggta aaacgcgcca acgccgggaa
    56881 gctcgatggc gtgaatgtgt tattacgccc ggtgagtgct gaatcgctgg ataacctggt
    56941 ggcaacctcc accgcgccgt ttatcacgca tgaaacggcg cgagcggcac aatcactaaa
    57001 cagtccggcc cccggcggat tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca
    57061 gccctggcct tcggcatcac tttacgacta cccgccgcaa gaacagtgga acgctttcca
    57121 gaaactggca caaatgctga tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat
    57181 cttcactgac gccaatggta cgcagcatat tggccttcat ccgatcccgg atcgttccgg
    57241 cctgtggcgc tatctcagca ccacattgct gctactgacg atgctgggta gcgccattta
    57301 caatggcgta caggcctggc gtcgttacca gcgtcatcgc actcgcatga tggagattca
    57361 ggcctattat gaaagctgcc tgaacccgca actgatcacc ccttcagaaa gccttatcga
    57421 ataacacgtt tgcgcggcag gttatgctac cctgtcgcgc aaattgcttc actctggaga
    57481 tttccctcat gcatatcaac attgcctggc aggacgtaga taccgttctg ctggatatgg
    57541 acggcacgtt gctcgacctc gccttcgata actatttctg gcaaaagctg gtgcctgaaa
    57601 catggggcgc gaaaaacggg gttacgccac aggaagcgat ggaatatatg cgccagcaat
    57661 atcacgacgt acagcatacg ctaaactggt actgtcttga ttactggagt gagcaactgg
    57721 gtctggatat ctgtgcgatg accaccgaga tgggaccgcg cgccgtactg cgtgaagata
    57781 ccattccgtt tcttgaggca ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg
    57841 ctcatccgca caacctggcg gtaaaacttg agcatacggg tctggacgca caccttgatt
    57901 tattactttc cacccacaca tttggttatc cgaaagagga tcagcggtta tggcatgcgg
    57961 tggccgaagc tacgggtctg aaagctgaaa gaacgctgtt tattgatgac agcgaagcga
    58021 ttctcgatgc tgccgcgcaa tttggtattc gttactgcct cggcgtgact aatcctgatt
    58081 ccgggattgc cgagaaacag tatcaacgcc atccgtcact gaatgactac cgccgcctga
    58141 tcccctcgct aatgtgaagg agacgccatg aaagagaaac ctgctgttga ggttcgactg
    58201 gataaatggc tatgggctgc ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt
    58261 gaaggcggta aggtgcatta caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg
    58321 aatgccacgc tcactctgcg ccagggaaat gacgaacgca cggtgattgt aaaggcgatt
    58381 actgaacagc gtcgccccgc cagcgaggca gccttgctgt atgaagagac tgcggaaagt
    58441 gtagagaaac gcgaaaaaat ggcgatggca cgtaaactta atgccttaac catgccgcac
    58501 ccggaccgac gcccggacaa aaaagagcgc cgcgacctgt tacgatttaa acacggcgac
    58561 agtgaataac tgtcacctgc aagagagatg attatgccgc aacatgacca attacatcgc
    58621 tatctgtttg aaaattttgc cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa
    58681 cagatccttg agaaccacga ttatccgcag cccgttaaaa acgtgctggc agaactgctg
    58741 gttgcgacca gcctgttaac cgctacgctg aagtttgatg gtgatatcac cgtacagctg
    58801 cagggcgacg gtccgatgaa tctggcggtt attaacggta acaataacca gcaaatgcgc
    58861 ggtgtggcgc gcgtgcaggg cgaaattcca gaaaatgccg acctgaaaac gctggtcggc
    58921 aatggttacg tggtgatcac cattaccccg agcgaaggcg aacgctatca gggcgtagtt
    58981 ggtctggaag gtgataccct ggcggcctgc ctagaagatt actttatgcg ttctgaacag
    59041 ctgccgacgc gcctgtttat tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt
    59101 atgttgttgc aggtaatgcc tgcgcaaaat acccaacagg acgactttga ccacctggcg
    59161 acgctaaccg aaaccatcaa aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg
    59221 tggcgtttgt atcacgaaga agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa
    59281 tgcacctgct cgcgtgaacg ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt
    59341 gatagcatcc tggcggaaga tggcgaaatt gacatgcatt gtgattactg cggtaaccac
    59401 tatctgttca atgcgatgga tattgctgaa atccgcaaca acgcgtctcc ggcagatccg
    59461 taagttcatt aatgagtatg tccggcagag agggctctgc cggattttac gacttaatgg
    59521 cctgatgtgc tgcgcttacc agcgcctatg aaataaatcg caaattattt tggtcagata
    59581 cagcgtcgta tccgataaaa gcgttgtcac ttacttaagt ttatttccag cgtttcacca
    59641 tctaccgtca gatcgcgcca cgctttcggc ctcgtcccca gttttccatc cttcgcagct
    59701 gtgagtaatt tttctttcgt tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat
    59761 tcttttctgt ctgagtcaaa gccatagaca atgactctgt catcattaaa agcaaacagg
    59821 atccgctccg cctgaccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt
    59881 tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc
    59941 ggtttaacag aaccaggggc aattaacaca ttttcggcga atactttttc cgatacctgt
    60001 tcctgaagat gttgctctcc atctaacgcc atcagcagat cccgcgcgcg tttcgggtct
    60061 ttcataaacc cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc
    60121 tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc
    60181 atatgactgt tcacgctaat acgcatactg tccagcaccg gcgaattaag aagcaccagt
    60241 atcaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt
    60301 tgcccattac gccagacgat actgacaaaa taccccagcg accacaccag taacaccagc
    60361 tccgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat
    60421 aacccccagg tggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg
    60481 caacgcaacg gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt
    60541 aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa
    60601 atcgcgctca ggcccgtaaa cggcagggtg atgataaaca tcagggttag caatgatact
    60661 aacggcagca accccgtggc gattagcgtg aacaactttt gaatagagtc gattaaacgt
    60721 gactgtgtac gcgcgaggat caccgccagt gcggtaacca gaccaaatgt aagatacata
    60781 aaccagtcgg ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag tttaaacaac
    60841 tcaccccaca gtaaaagcac cagccacgtc aggccattgg agagaaaaat caccagtaat
    60901 ataagtacgt tatgccatac tgactggtaa aagtagctat agcggctact gccattgcgg
    60961 atacgaaggc tttgttgtat ccacggtagc aacaacattg ccatcaacag cagatagcag
    61021 ccaaaatccc aaatcgcctt ttcagctctc caggggttca tgccatcagt ttgccacttc
    61081 agccaaccgc tcatccctgt cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc
    61141 ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc
    61201 acgccataaa caatccaact gtgatttttt ccggcaagcc aggcgataag caggtagcaa
    61261 accagcccct gaagtaatcc ggtagcaatc atcccctgaa gtaatccggt agcaatcatc
    61321 ccccagcgtg ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag
    61381 gtgggttaat tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa
    61441 aagttagcgt ggtgaatcga tactttaccg gttgaatttg catcaatttc attcaggaat
    61501 gcgattccac tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt
    61561 tccacaccta aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga
    61621 gttttttgtc aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa
    61681 cacccccaaa aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc
    61741 gcgttaacaa tggtttgccc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg
    61801 atatcgttta caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga
    61861 caggttatga gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct
    61921 tcaccggtcg ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt
    61981 tctggtgggc agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct
    62041 ggcagcatct gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg
    62101 acgctttctg tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg
    62161 cctggcaggc gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag
    62221 gtttcaaacc agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag
    62281 aacagggtct gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga
    62341 ttggcggcac ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc
    62401 tgctgccgct gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg
    62461 atgttgcggt gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga
    62521 aacgccgcct gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaatttcg
    62581 aaggcggctg ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca
    62641 acgctatccg tcgtgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg
    62701 actttgatga tggttcaaaa accgagaaca cccgcgtttc ttatccaatc tatcacatcg
    62761 ataacatcgt taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg
    62821 ctgatgcttt cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc
    62881 acttcctctc tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga
    62941 cgccaacctt ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg actcagtacg
    63001 cagaagtgct ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg
    63061 gctggaacgg cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca
    63121 tcctcaacgg ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga
    63181 tcccaaccga actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt
    63241 ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg
    63301 ataaatacac cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat
    63361 gatttgaagc tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt
    63421 cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcactggc
    63481 agccaggcgc gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta
    63541 tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct aatggtgcgc
    63601 gcactgtcgc cgcggacaaa cggctggaac aggtgcttac gttgttccgg cgcaattccc
    63661 ggaccgtcat cttccacctg gaaccaggcg cgattcggct ccgttccgct gctgactttg
    63721 atccagccat tgccataacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc
    63781 gacagcgggt gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc
    63841 tcatagccac tttcggcagc aatcacctca ccgagcactg cattaagatc cgccatttcc
    63901 atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac
    63961 tcttcgatat ctttattgat cgattctgcc agatagccat cctgctcgct catcatctca
    64021 gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc
    64081 agcgtgcggt catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcacgggta
    64141 acggaacgca cctccgaagc gccatattca cgcagcggcg gcggaataat ccctttacca
    64201 acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc
    64261 cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag cggagagaaa
    64321 tcgccctgat gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt
    64381 ttcagccaga cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc
    64441 agttgctgtg ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc
    64501 tcttcggcag cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg
    64561 aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac
    64621 atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag
    64681 ttcagcacca ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat
    64741 aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt
    64801 ccggtacaaa gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat
    64861 cttcttctac catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg
    64921 aatattcacg accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat
    64981 ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcatctt
    65041 cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct
    65101 cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac
    65161 gggccagcag ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca
    65221 ggcctacgat acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt
    65281 tgctctgact acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat
    65341 ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag
    65401 cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc
    65461 gcatgtcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag
    65521 gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat
    65581 ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct taagtataca
    65641 atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc
    65701 attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg
    65761 tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca
    65821 aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat
    65881 aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa
    65941 aatctcaaaa tcgtcgatta ctcccctcag caggaaggca aagtcttttt tggcgcgtgg
    66001 gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa
    66061 atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa
    66121 aaagaagtcg gcgatctggc ggtggtgaat acccctgccg gggaagcgaa ctggtatgtt
    66181 aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca
    66241 tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa
    66301 accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa
    66361 caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt
    66421 tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt
    66481 ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc catttccgag
    66541 caaggcaaac tcaccgatga tctggcgaag gccatcaacg ccaccctaag caaaaccgaa
    66601 ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt
    66661 gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa
    66721 gtcgccgctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa agccgcgctg
    66781 gatggcgcgc gctatatcct gatggaacgg tttgccgaag atgccacgct gctggcgaaa
    66841 gtgcgtgatt atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa
    66901 gaggaagggg cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct
    66961 tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtactcca gctttcgctg
    67021 aatgccgatc cgcagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg
    67081 gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aagcgtagtg
    67141 agctggacct ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggtaccgtg
    67201 cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg
    67261 atggcggccc ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg
    67321 gtaaaagtgg cggtggtcga tgccactggc aaactggtgg cgaccgatac catttacccg
    67381 cacaccggac aagccgcaaa agcagcgatg accgttgctg ccttgtgtga aaaacataac
    67441 gttgaactgg tggcgatcgg caacggtaca gcttcccgcg aaactgaacg tttctatctc
    67501 gacgtgcaga agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc
    67561 gcgtcggttt actcagcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg
    67621 ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa
    67681 atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg
    67741 gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac
    67801 accgcttctg ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac
    67861 atcgtggcct ggcgcgatga gaacggccag ttccagaacc gtcagcaact gctgaaagtc
    67921 agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt
    67981 gataacccgc tggacgcctc taccgttcat ccggaagcct atccggtggt ggaacgcatt
    68041 ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac
    68101 ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc
    68161 aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc
    68221 gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgcagtgacc
    68281 aacgtcacca actttggcgc gtttgtcgat attggcgtgc atcaggacgg cctggttcac
    68341 atctcttcat tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac
    68401 attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg
    68461 cgtctggacg agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa tgaacgcccg
    68521 caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaatagc
    68581 gcgatgatgg atgcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc
    68641 cctaaacctg cgcgataagg cgttcacgcc gcatctggca acgaacgctt tgcctgacat
    68701 aaaagtgccg gagaatatct ccggcacttt tattccacag ccaaactcac aatatactcc
    68761 gttaatattt atcacttcat taacaactga aaccttaatt aaacattagc cagtccgggt
    68821 aactcactat tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag
    68881 tcagattatt attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt
    68941 ctcattatca ttgtgttgtt gattatttaa tctctccttt gttggcaaat catctggtct
    69001 catgtcgcgg tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac
    69061 ctatgcaata cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc
    69121 cggcatatcg ccaaaaactg ctttctctag gcatgttacc tggctcctct tttgatgtgg
    69181 tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat
    69241 tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata
    69301 acaacaatga aaaaattaac cattggctta attggtaatc caaattctgg caagacaacc
    69361 ttatttaacc agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc
    69421 gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc
    69481 ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt
    69541 cactacattt tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt
    69601 gagcgtaacc tgtacctgac gctgcaactg ctggaactcg gcattccctg catcgtggcg
    69661 ctgaacatgc tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctatcg
    69721 gcacgtcttg gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg
    69781 ctcaagctgg cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca
    69841 cagccgctac tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg
    69901 aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac
    69961 gccggtgaag cgtcgcagca tctggatgcc accctcgccc gtctgcgtaa tgaaatggat
    70021 gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg
    70081 gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg
    70141 ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg
    70201 gctatcaaca tcggcggggc gttacagccg ctgtttaacg tcggctccgt ggcgctattt
    70261 gtgcatggta ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc
    70321 ctcgcccagg gcctgggtgg cggtattaac accgtgctgc cactggtgcc gcagatcggc
    70381 atgatgtacc tgttcctctc cttccttgag gactccggct atatggcgcg tgcagcgttt
    70441 gtgatggacc gcctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc
    70501 gtcggtttcg gttgtaacgt accgtcggta atgggcgcac gtacgcttga tgcaccgcgt
    70561 gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc
    70621 ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg ccctggcggt tttctcgctg
    70681 tatatgctcg gtatcgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg
    70741 cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa
    70801 agcctgatta tccagacctg gcagcgtctg aagggcttcg ttctgcgtgc tggtaaagtg
    70861 attatcatcg tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc
    70921 gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgataac ccctgtcttc
    70981 aagccgattg gcgtacatga agataactgg caggcaacgg ttggcctgtt tacgggcgca
    71041 atggcaaaag aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag
    71101 gacgaagagt tcaatccggc ggaattcaac ctcggtgaag agctgttcag tgcggtagat
    71161 gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa
    71221 gctagcaaag gcaacggtga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc
    71281 ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc
    71341 tcggtgatgg gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg
    71401 tgggggctga atatcgctta ctcactggca acattgttct atcaggtcgc cagctacagt
    71461 cagcacccaa cttacagcct ggtgtgcatt ctggcggtaa tcctgtttaa catcgtagct
    71521 atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag
    71581 tcggtaagca gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta
    71641 ttcaggtacg tgatttgctg gcgttacggg gccgtatgga agcgacccag ataagccaga
    71701 cattgaacac tccgcagcca atgattaacg ccatgctgca acagctggaa agcatgggca
    71761 aagccgtgcg gattcaggaa gaacctgacg gctgcctctc tggcagttgt aaaagctgcc
    71821 cggaaggcaa agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct
    71881 gttttgaaaa gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa
    71941 aatgcaattc ttgcagcgca acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact
    72001 cgctaatgca cagatgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg
    72061 aacgccatga gtaaaaagca gagttccacc ccacatgatg cgctgttcaa actcttttta
    72121 cgccaaccgg agacggcacg cgattttctt gcgtttcatt taccggcacc cattcacgcg
    72181 ctctgtgata tgaaaaccct caagctggag tcgaggagct ttattgatga cgatctgcgt
    72241 gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg atacatctat
    72301 tgtctgattg aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac
    72361 gctattgccg caatgcaaaa tcaccttgat gctggataca aaacgttgcc gatggtggtg
    72421 ccattgttgt tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat
    72481 tgtttcgccg atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat
    72541 gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac gtatggcgct gctggagtta
    72601 attcaaaaac atattcgtca acgcgatctg atggggctgg tagagcaaat ggcctgctta
    72661 ttaagtagtg gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa
    72721 actggcgacg ctgtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa
    72781 cacaaggaga gtttaatgac tattgcggaa agattgcggc aggaggggga acaatccaaa
    72841 gccctgcata tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcga
    72901 tttaccgggc tgtcagaaga agagttggct gcggcgagta gactggcccc ctgaatctcc
    72961 agacaaccag
//
LOCUS       DAJTRN010000004        72115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRN010000004 DAJTRN010000000
VERSION     DAJTRN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1389..2975)
                     /locus_tag="OO328_000270"
     CDS             complement(1389..2975)
                     /locus_tag="OO328_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5928969.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3330..5501)
                     /gene="glcB"
                     /locus_tag="OO328_000271"
     CDS             complement(3330..5501)
                     /gene="glcB"
                     /locus_tag="OO328_000271"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5928970.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5523..5927)
                     /locus_tag="OO328_000272"
     CDS             complement(5523..5927)
                     /locus_tag="OO328_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5928971.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRATKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5932..7151)
                     /gene="glcF"
                     /locus_tag="OO328_000273"
                     /pseudo
     CDS             complement(5932..7151)
                     /gene="glcF"
                     /locus_tag="OO328_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7162..8214)
                     /gene="glcE"
                     /locus_tag="OO328_000274"
     CDS             complement(7162..8214)
                     /gene="glcE"
                     /locus_tag="OO328_000274"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5928972.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8214..9713)
                     /gene="glcD"
                     /locus_tag="OO328_000275"
                     /pseudo
     CDS             complement(8214..9713)
                     /gene="glcD"
                     /locus_tag="OO328_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9964..10734
                     /gene="glcC"
                     /locus_tag="OO328_000276"
     CDS             9964..10734
                     /gene="glcC"
                     /locus_tag="OO328_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5928973.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10858..11999)
                     /locus_tag="OO328_000277"
                     /pseudo
     CDS             complement(10858..11999)
                     /locus_tag="OO328_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12361..14091
                     /locus_tag="OO328_000278"
     CDS             12361..14091
                     /locus_tag="OO328_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5928974.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14088..15002
                     /locus_tag="OO328_000279"
     CDS             14088..15002
                     /locus_tag="OO328_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5928975.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15035..15283
                     /locus_tag="OO328_000280"
     CDS             15035..15283
                     /locus_tag="OO328_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5928976.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15283..16454
                     /locus_tag="OO328_000281"
                     /pseudo
     CDS             15283..16454
                     /locus_tag="OO328_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16490..17569)
                     /gene="lptG"
                     /locus_tag="OO328_000282"
     CDS             complement(16490..17569)
                     /gene="lptG"
                     /locus_tag="OO328_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5928977.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17566..18636)
                     /gene="lptF"
                     /locus_tag="OO328_000283"
     CDS             complement(17566..18636)
                     /gene="lptF"
                     /locus_tag="OO328_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5928978.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18667..19227)
                     /locus_tag="OO328_000284"
     CDS             complement(18667..19227)
                     /locus_tag="OO328_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5928979.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19239..20063)
                     /locus_tag="OO328_000285"
     CDS             complement(19239..20063)
                     /locus_tag="OO328_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928980.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20063..21412)
                     /locus_tag="OO328_000286"
     CDS             complement(20063..21412)
                     /locus_tag="OO328_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5928981.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21458..22216)
                     /locus_tag="OO328_000287"
     CDS             complement(21458..22216)
                     /locus_tag="OO328_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5928982.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22248..22961)
                     /locus_tag="OO328_000288"
     CDS             complement(22248..22961)
                     /locus_tag="OO328_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5928983.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23135..23827
                     /locus_tag="OO328_000289"
     CDS             23135..23827
                     /locus_tag="OO328_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5928984.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23876..25375)
                     /gene="pitB"
                     /locus_tag="OO328_000290"
     CDS             complement(23876..25375)
                     /gene="pitB"
                     /locus_tag="OO328_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5928985.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25667..27526)
                     /gene="gss"
                     /locus_tag="OO328_000291"
     CDS             complement(25667..27526)
                     /gene="gss"
                     /locus_tag="OO328_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5928986.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27731..28597
                     /gene="yghU"
                     /locus_tag="OO328_000292"
     CDS             27731..28597
                     /gene="yghU"
                     /locus_tag="OO328_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5928987.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28720..28968)
                     /gene="hybG"
                     /locus_tag="OO328_000293"
     CDS             complement(28720..28968)
                     /gene="hybG"
                     /locus_tag="OO328_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5928988.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29102..29443)
                     /gene="hypA"
                     /locus_tag="OO328_000294"
     CDS             complement(29102..29443)
                     /gene="hypA"
                     /locus_tag="OO328_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5928989.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29436..29924)
                     /gene="hybE"
                     /locus_tag="OO328_000295"
     CDS             complement(29436..29924)
                     /gene="hybE"
                     /locus_tag="OO328_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5928990.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29917..30411)
                     /locus_tag="OO328_000296"
     CDS             complement(29917..30411)
                     /locus_tag="OO328_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5928991.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30411..32114)
                     /gene="hybC"
                     /locus_tag="OO328_000297"
     CDS             complement(30411..32114)
                     /gene="hybC"
                     /locus_tag="OO328_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5928992.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32111..33289)
                     /gene="hybB"
                     /locus_tag="OO328_000298"
     CDS             complement(32111..33289)
                     /gene="hybB"
                     /locus_tag="OO328_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5928993.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33279..34265)
                     /gene="hybA"
                     /locus_tag="OO328_000299"
     CDS             complement(33279..34265)
                     /gene="hybA"
                     /locus_tag="OO328_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5928994.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34268..35386)
                     /gene="hybO"
                     /locus_tag="OO328_000300"
     CDS             complement(34268..35386)
                     /gene="hybO"
                     /locus_tag="OO328_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5928995.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35575..35862)
                     /locus_tag="OO328_000301"
     CDS             complement(35575..35862)
                     /locus_tag="OO328_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5928996.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35981..36868)
                     /locus_tag="OO328_000302"
     CDS             complement(35981..36868)
                     /locus_tag="OO328_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5928997.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37025..38065
                     /gene="gpr"
                     /locus_tag="OO328_000303"
     CDS             37025..38065
                     /gene="gpr"
                     /locus_tag="OO328_000303"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5928998.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38105..38599)
                     /locus_tag="OO328_000304"
     CDS             complement(38105..38599)
                     /locus_tag="OO328_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5928999.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38790..39674
                     /gene="yghA"
                     /locus_tag="OO328_000305"
     CDS             38790..39674
                     /gene="yghA"
                     /locus_tag="OO328_000305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5929000.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39946..40371)
                     /gene="exbD"
                     /locus_tag="OO328_000306"
     CDS             complement(39946..40371)
                     /gene="exbD"
                     /locus_tag="OO328_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5929001.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40378..41112)
                     /gene="exbB"
                     /locus_tag="OO328_000307"
     CDS             complement(40378..41112)
                     /gene="exbB"
                     /locus_tag="OO328_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5929002.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41364..42551
                     /gene="metC"
                     /locus_tag="OO328_000308"
     CDS             41364..42551
                     /gene="metC"
                     /locus_tag="OO328_000308"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5929003.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42691..43350
                     /locus_tag="OO328_000309"
     CDS             42691..43350
                     /locus_tag="OO328_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5929004.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43390..44289)
                     /gene="yqhC"
                     /locus_tag="OO328_000310"
     CDS             complement(43390..44289)
                     /gene="yqhC"
                     /locus_tag="OO328_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5929005.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44483..45646
                     /gene="yqhD"
                     /locus_tag="OO328_000311"
     CDS             44483..45646
                     /gene="yqhD"
                     /locus_tag="OO328_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5929006.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45751..46578
                     /gene="dkgA"
                     /locus_tag="OO328_000312"
     CDS             45751..46578
                     /gene="dkgA"
                     /locus_tag="OO328_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5929007.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46778..47704
                     /locus_tag="OO328_000313"
     CDS             46778..47704
                     /locus_tag="OO328_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5929008.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47755..48012
                     /gene="yqhH"
                     /locus_tag="OO328_000314"
     CDS             47755..48012
                     /gene="yqhH"
                     /locus_tag="OO328_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5929009.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48055..50274)
                     /locus_tag="OO328_000315"
     CDS             complement(48055..50274)
                     /locus_tag="OO328_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5929010.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50385..51797)
                     /gene="ftsP"
                     /locus_tag="OO328_000316"
     CDS             complement(50385..51797)
                     /gene="ftsP"
                     /locus_tag="OO328_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5929011.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51872..52609)
                     /gene="plsC"
                     /locus_tag="OO328_000317"
                     /gene_synonym="parF"
     CDS             complement(51872..52609)
                     /gene="plsC"
                     /locus_tag="OO328_000317"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5929012.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52843..55101)
                     /gene="parC"
                     /locus_tag="OO328_000318"
     CDS             complement(52843..55101)
                     /gene="parC"
                     /locus_tag="OO328_000318"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5929013.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55647..56129)
                     /locus_tag="OO328_000319"
     CDS             complement(55647..56129)
                     /locus_tag="OO328_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5929014.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56182..56574)
                     /gene="ygiW"
                     /locus_tag="OO328_000320"
     CDS             complement(56182..56574)
                     /gene="ygiW"
                     /locus_tag="OO328_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5929015.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56726..57385
                     /gene="qseB"
                     /locus_tag="OO328_000321"
     CDS             56726..57385
                     /gene="qseB"
                     /locus_tag="OO328_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5929016.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57382..58656
                     /gene="qseC"
                     /locus_tag="OO328_000322"
     CDS             57382..58656
                     /gene="qseC"
                     /locus_tag="OO328_000322"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5929017.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58702..59034)
                     /locus_tag="OO328_000323"
     CDS             complement(58702..59034)
                     /locus_tag="OO328_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5929018.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59353..59934
                     /gene="mdaB"
                     /locus_tag="OO328_000324"
     CDS             59353..59934
                     /gene="mdaB"
                     /locus_tag="OO328_000324"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5929019.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59965..60279
                     /locus_tag="OO328_000325"
     CDS             59965..60279
                     /locus_tag="OO328_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5929020.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60327..62219)
                     /gene="parE"
                     /locus_tag="OO328_000326"
     CDS             complement(60327..62219)
                     /gene="parE"
                     /locus_tag="OO328_000326"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5929021.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62248..62829)
                     /gene="yqiA"
                     /locus_tag="OO328_000327"
     CDS             complement(62248..62829)
                     /gene="yqiA"
                     /locus_tag="OO328_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5929022.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62829..63656)
                     /gene="cpdA"
                     /locus_tag="OO328_000328"
     CDS             complement(62829..63656)
                     /gene="cpdA"
                     /locus_tag="OO328_000328"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5929023.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63681..64103)
                     /locus_tag="OO328_000329"
     CDS             complement(63681..64103)
                     /locus_tag="OO328_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5929024.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64104..64733)
                     /gene="nudF"
                     /locus_tag="OO328_000330"
     CDS             complement(64104..64733)
                     /gene="nudF"
                     /locus_tag="OO328_000330"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5929025.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64938..66419
                     /gene="tolC"
                     /locus_tag="OO328_000331"
     CDS             64938..66419
                     /gene="tolC"
                     /locus_tag="OO328_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5929026.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66567..67238
                     /locus_tag="OO328_000332"
     CDS             66567..67238
                     /locus_tag="OO328_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5929027.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVLMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67244..68404
                     /locus_tag="OO328_000333"
     CDS             67244..68404
                     /locus_tag="OO328_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5929028.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68442..69230)
                     /gene="ygiD"
                     /locus_tag="OO328_000334"
     CDS             complement(68442..69230)
                     /gene="ygiD"
                     /locus_tag="OO328_000334"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5929029.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69373..70146
                     /gene="zupT"
                     /locus_tag="OO328_000335"
     CDS             69373..70146
                     /gene="zupT"
                     /locus_tag="OO328_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5929030.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70204..70374)
                     /locus_tag="OO328_000336"
     CDS             complement(70204..70374)
                     /locus_tag="OO328_000336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929031.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70635..71288)
                     /gene="ribB"
                     /locus_tag="OO328_000337"
     CDS             complement(70635..71288)
                     /gene="ribB"
                     /locus_tag="OO328_000337"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5929032.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71393..71541)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71663..71953
                     /gene="ubiK"
                     /locus_tag="OO328_000338"
     CDS             71663..71953
                     /gene="ubiK"
                     /locus_tag="OO328_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5929033.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18045 a        19160 c        17744 g        17166 t
ORIGIN      
        1 ttacgtaccg tttaaacaaa gacaatacgt ctctttattt ctcataaaac cccaatccct
       61 tacagggctt attgaaaaat ttaaaaatca caaatttctc aatactcgaa atgagatcac
      121 ataagaaatc aaaaagcctt ctcatctatt ttaagttttc ccaacggaaa ataagagctt
      181 tcattaatac tgaaccactt tactgttcta tttgttcagg tattgcatta cggtgtgctg
      241 agatccatcg gtgcatccct gtatcccgcg atgagactaa aatagtcgtg atgtaagctc
      301 gtatcagatt cttagtgaat accacatttg ttataattaa gtgaaaagat acattgctga
      361 tttacattat ttggtttaca aaatgtcatc acttgcagag ttatcgctga cagatacctt
      421 taatctctta ctactttcgt gttattgcaa tacaaccatc cgtaaagcag ggctattttt
      481 agcaggaaat actcaggtgt caacattcga actccaactc tgaaatattc atataagtta
      541 atgattttaa tgattttaat gattttaatg attttaatga ttttaatgat tttaatgatt
      601 ttaatgattt taatgatttt aatgatttta atgatttttt catttattca cacctaataa
      661 aagtattcaa attcagatga tttttctgct caacagacac aaacaggcaa taatactctc
      721 caaagcgaac aatatttggc attacgattt aatgcttcat aatcaccaaa ttatttaacc
      781 ataaattttc ttatttctaa caaaatgatt ggtgtgttgt tggagttaaa atacatacat
      841 aatattaaca tctgccaaca tttcactttc cgcaagacgg atgatttcga atcctcatcc
      901 acgttaccct atcccaaata tctttattaa taggcagtaa cgaacactgt tatagcaaga
      961 ttaacttatt aacctaacgg catcttttat gtaacaagtg gagataaatt gcttaaaatt
     1021 gatcctcgac aaatcttaaa agttctcatt attctgcaaa atcagctttg cgaataatct
     1081 tatgtttttg ggagttttgg gaattttgat ctactcattg ttctagtgaa aaatataaat
     1141 aagagaaaaa acgccattcg tcatgaaagc aacagatgaa gcaatgtaat aacatactaa
     1201 aattgatctc atgaaagtgg gtactttata accagcacag aagccaagtt gtataaatat
     1261 taaacaattc gcatcgtaaa acggagacta cgtataaaga agccaatcgt aaaaacggtt
     1321 ccctggcgag ataaacgaca gacaaatgga aagccgggtg atatttacct tcatccggct
     1381 ttattgattt acgagactaa catcccggta aacacatacg cctgcagcag ggtgataacg
     1441 ctggcaaaaa tcagactgtg cttcacggtg tagcggaaca gttcagattc tcggcccacc
     1501 atgcccgtcg cggcgcaggc cacggcgata gattgcgggg agatcatctt gccagttacg
     1561 ccgccgctgg tatttgctgc caccagcaga gtgtcagaga cgttgatttg ctgcgccgtg
     1621 gtcgattgca gtgaaccaaa cagggcgttt gaagaagtgt ccgacccggt aaggaatacg
     1681 cccagccagc cgagaaacgg tgagaaaaac gggaacatca cgcctgtacc tgccagtacc
     1741 agcgccagcg tggtggacat gccggaatag ttggtgacga aggcgaatgc cagcaccatg
     1801 ccaatcgaca atatcggcca cttcagacta attagcattt cggcaaagac gccaatccct
     1861 ttcttgatgc ccacgccgag gatgaagata gagataatcg ccgcaataaa aatagcggtg
     1921 ccgccagccg agagagggtc gaatttaaac accgcatcca ttggcgttgg ttgggcgaca
     1981 atgggtgccg ctttcaacac ttgttgatgc aaatgaggga tctggaaatt aatcaccagt
     2041 gaataaaacg cgccgcccgg agcaaataac gctttaaacg gcttcatggt ccagatggtg
     2101 accagcaccg ttaagattaa aaacggtgac cacgcacgaa tgatttgccc cagactatat
     2161 tctgaaggca cgggaccgcc agaagatggc ttatttacca ccatcgcacc tgcggattgc
     2221 cccatgctga ttgccgtttc ggtatttttc ggccgccaga ctttaaggaa taaagcgagt
     2281 gagacgatac tcaccagcgc cgaagtaata tccggcagtt ccggaccaat atagttagag
     2341 gtaaagaact gagtgacagc gaagcttccc ccagcaacca gcgccgctgg ccacgtctct
     2401 ttcacccctt tccagccgtc catcattgct accagccaga acggcacaag aaccgacagg
     2461 aacggtaact gacgtcccgc cattgcgcca atgtggaacg gatcgattcc cgttacctga
     2521 ccggcgacca gaatcggcac gcccaacgca ccaaacgcca ccgtcgcacc aaagccagca
     2581 gctccttcca gcaacgcacc aaaggagaaa ccaatcagta acacctgcaa acgctgatcg
     2641 tcggtgatgg agataaccga gctgcggata atatcgaact gcccgctggc aacggttaat
     2701 ttatacagga acaccgccgc gacaataatc cacgcaattg gccataatcc ataaataaag
     2761 ccatagcccg cagcagcaaa tgccatatca atcggcattt taaaggcgaa tatcgcaatc
     2821 aggatagata atataagggt tattgctcca gcaacatgtc ctttcagacg taataccgcg
     2881 agtgcaacga agaagaatat tatcgggatc agggcgacca gagcggatag ccccagtcct
     2941 cccatcggca tatacatttg ggtccaggta accatattgt tcagtctctt attatcttta
     3001 tatgcttgat atacttaagg ttgtaataag caaaagagga ctgaactgta aaatataggc
     3061 gttatacttt acagcaacag tacgccgcta acgcaattgc tacctctggc ataacaagta
     3121 tatcgggtaa gggtttctgt tccgcacacg caggcgcaga gtatcgttaa gatgtccata
     3181 ttgttgtttt aggcccgcta gtaatgcgct acgggtattt aatattgtta aaccctgata
     3241 atcgctccgg ttatttccgg gataaatgta ctaccgcagt tactatcata gccccgacaa
     3301 taaaacttgc cggggctttt ttgacgctat taatgacttt ctttttcgcg taaacgccag
     3361 gcgtgtaata acggttcggt ataaccgttt ggctgtttca cgccgaggaa gattaaatcg
     3421 ctggcagctt taaaagcaca cgagttagcg aaattccccg ccatcggacg ataagccgga
     3481 tcgccagcat tttgctgatc aaccactttc gccatattct ccagcgacgc ctgcacctgt
     3541 tctttggtca gaataccgtg acgtaaccag ttggcgatat gctggctgga gatacgcagc
     3601 gttgcgcggt cttccatcag cgccacgttg tgaatatccg gcacttttga acaaccaatc
     3661 ccctgctcca cccaacgcac cacgtacccc agaatcccct gcacgttgtt atccagctct
     3721 tgttggatct cttgcgccga ccagttagcg ttttcagcca ccggaatagt cagcagatcg
     3781 tccagcagcg gttcaaattc agcactgaac tcggtctggg caatgttggc ttgcacgctc
     3841 tgtacgttgg tatggtggta gtgcagcgca tggagcgtag cagcggttgg tgacggaacc
     3901 caggctgtgt ttgccccggc acgcagttgg tcgcccttct ggctgtacat gtctgccatc
     3961 aggtccggca ttgcccacat gcctttaccg atttgcgctt taccgcgcag cccacagaac
     4021 agaccggaaa gcacgttatt acgttcgtag gctttgatcc acggcgtcga tttcatctgg
     4081 tttttacgca gcatcgggcc agcttccatc accgaatgca tttcatcgcc ggtacggtcg
     4141 aggaaaccgg tattgatgaa cgccacgcgg ttgcgcgcct gagcgataca gctacgcaag
     4201 ttcagcgagg tccggcgttc ttcatccata atgcccattt tcagggtatt cggtgccata
     4261 ccgagcattg tctcaatgcg ggtaaacagt ttgttggcga acgccacttc ctgcggaccg
     4321 tgcattttcg gtttcacaat atagacgctg ccagtgcgcg agtttttctg cacttttaaa
     4381 tcatagaggg caatcgcgcc agtcatgacg ccatcaagaa taccttccgg gatttcattg
     4441 ccttcgctgt cccaaatcac aggaatggtc atcaaatgac ccacgttgcg gataaacagc
     4501 aacgagcgtc cgtgcagaga aatttcagag ccatcggcgg cggtgtactg acgatcgtca
     4561 ttcagtttac gtacgatttg ccgaccgttt ttctccattt tctcttgcag agtcccctgc
     4621 atcaggccca gcagattgcg gtacagcagg attttatctt ccgcatcaac cgccgcgacc
     4681 gaatcttcgc agtcgagaat ggtactgata gcagcttcga cgataacatc gttgatgtgc
     4741 gccggatcgt ctttgccaat ccgcccgttg gcatcaattt gcagttcaat atgcaggccg
     4801 ttatttttca gcaaaatgca ggtcggcgca gcggcatcgc cacggtaacc gacaaactgt
     4861 gctggagtac gtaacgtggt ttctttacca tttttcaact ggatgcgtaa ttgtttatcg
     4921 accaccttaa acgccaccac atcctgatag ctgccgtttt ccagcggtag tgattcatcg
     4981 aggaaacgtc gaacccaggc gataacctgc tcaccgcgct gcggatcgta gccactgacc
     5041 atcgcccctt cctgcgggat gatgtcgctg ccgtataacg catcgtacag tgagccccag
     5101 cgagcgttcg ccgcgttcag cgcgtagcgg gcgttcattg ccggaaccac cagctgtggc
     5161 cccgcctgac tggtgatttc gctgtcaatg cccgtggttt ccaccgtcac gcgctccggt
     5221 tgcggcacca ggtagcccag ttcacgaagg aaagatttat aggccgcttt atcttttacc
     5281 ggccccggat tgctgcgatg ccactcatca agcgccgcct gaatgcgatc gcgttctgcc
     5341 agcaactgac gattttctgg tgccagatcg tgaacgatct catcaacatt gtgccagaat
     5401 gcagcagcgt ccagttccac gcccggcaaa acttctccat ccacaaaacg tttaaaattg
     5461 gcgtcaatgc gtaaacggcc ctgggttatg gtttgactca ttgtttctct cctcgttttc
     5521 gcttatttcg ccaacaccgc tgctgccact ttcgcgacct gcgcatcctg tgctccggtt
     5581 aaaccagaaa cgcccacggc accaataatt tgcccatcaa caacaaccgg tacgccgcct
     5641 tccagcgacg tcagtaacgg cgcagtcacg aacgcggtac gtccgttgtt caccatctct
     5701 tcatagccct tagttgcacg acgccccagc gcggcggtac gcgctttctc ctgggagatg
     5761 taagccgcaa tcggcgcgca atcgtccatg cgacttaacg ccagcagatg accgccgtca
     5821 tcggcaacag caatggaaac agaccagtta tttttctgcg cctcttcctg acctgcggca
     5881 ataattgcac tcgccatttg ctggctaaga atgactttag ttttcatttt tttattcctt
     5941 ttcaagggct tgttctacaa tttcaatcca gtgacgcaca gaggtacgac cggcgctcgc
     6001 cagatgcgtc tggcaaccaa tgttggcggt gacgatcatt tccggtttgc cgctttccag
     6061 cgcattcatt ttgttatccc gcagctggcg tgccagatcg ggatgcgtta acgcatatgt
     6121 tcccgctgaa ccgcagcaca gatggctgtc gggaacgtcc gttaaggtaa atccaagacg
     6181 aagtaacact ttttccactt cgccgttcag cttttgcgca tgttgtaggg tacacgggca
     6241 gtggaaggcc agttttttat cgccgcgaat tgccagtttt tccagcggtt cctcgcgcag
     6301 aagttcgact aaatcgaccg ccagttcact gacctgacgc gctttatcgg catataacgc
     6361 atcgtttttc agcatctgcc catactcttt gacgaacgcg ccgcaaccgc tggcggtttg
     6421 caaaattgcc tcggcacctg cttcaatcgc gggccaccag gcatcaatat tattgcgcgc
     6481 ccgtgccaac cctttctcct gcgcattaag atgatagtcc accgcgccac aacagcctgc
     6541 ttcgttagct gacatgacgc tgatccccag acgatccagc actcgcgcag ttgccgcgtt
     6601 ggtgttgggc gaaagcgtag gctgggcgca gccttccagc attaaaaccc gacgcttatg
     6661 gcgcagcggc ggacgcggtt tagctttcac cgtttcagca ggcagttttg ctctgacctg
     6721 ttccggtaaa aacggtcgca gcaccagccc tacctgcgtc agcgcacgga agaccgccgg
     6781 acgcggcact acctggcgca atccttcgcg cagcattcgc tccggcagtg ggcgtttcac
     6841 tttctgctcg acaatatcac gcccgatatc cagcaaattg tgatagcgca caccagaagg
     6901 acaggtggtt tcacaattgc ggcaagtgag gcagcgatcg agatgctcct gtgttttaag
     6961 cgtgacttcg ttgccttcca gcacctgttt aatcagatag atgcgcccgc gcggcccgtc
     7021 cagttcatcg cccagaagct gataggttgg gcaggttgcg gtacaaaatc cgcagtgaac
     7081 acaggcgcac aggatgctgt cggcttccag cgcgcgttct gccgcatctc ttcggttaat
     7141 cgggtttgca tagcctgctc ctcaaagttc cgcgtacatg cgaccggggt taaacacgcc
     7201 gcaagggtcg agctgctgtt taagctgctg gtgatagcgg aataaaggag ccgatagcgg
     7261 ggcaaagcca ccatctccgg cactaaagcg ggtcgcatga ccgccagcgt tgcgggcgat
     7321 gcgatggatt tgattgtcat cggctgtcga tttcagccag cgtaacgccc cgccccagtc
     7381 gatcagttgc tcgccgggta aatccatcat cggcgcatcg ctgggtaatg aaatgcgcca
     7441 taaggtacct ggtaacgaga agaacggcag ttgttgttca cgcaattgct gccagaactg
     7501 acccacaacc tcttcgccac ccagcagttc acgcgctgct tttaccgatc cttcgccgcc
     7561 ctcaaggcgg atccacaacg cattgtcgaa gtaacataag ccactaatgg gtaatggctg
     7621 gagttgccac tcggcgattt cactcatggc ttcttgcagg ctgatttccc gacgcaggct
     7681 cagagaggca cgcggtcgcg gtaacacttt cattgagatt tcagtgagca cgccaagaca
     7741 accgtagctt ccggccatta accgtgagag atcgtatccg gcaacgtttt tcatcacttc
     7801 gccaccaaaa cgcagatgtt ttccagtgcc ggtaatgatg cgcgtgccga ggacaaaatc
     7861 gcggaccgaa ccgctccacg ggcgacgcgg ccccgccagc ccgcaggcga ccatcccgcc
     7921 ccaggtggct tcttcaccat aatgcggcgg ctcgcaggga agcatttgcc cggcgctctc
     7981 cagcgccgct tcaatcgcca ccagcggcgt tccggcacga gcggttatca ccagctcggt
     8041 cgggtcgtaa ttaacaatgc cgcgatgaca acgcacatcc agcgtttgcc cggtgactgg
     8101 gcgacctaaa aaggctttgc tattgctgcc ctgaatcacc agcggcgttt tatcgctaat
     8161 tgcctgattc acctgctcca gcagcgcctg actgtaatca cactcgcgta gcatcaaaag
     8221 cgctccagtt caggaaaagg taaatgaccg tgatgcacat gcatggcacc aaattcagca
     8281 cagcggtgta gcgtgggaat gtttttccca gggttcagca aaccatcggg gtcaaacgcc
     8341 gccttgaccg catggaaggt cgtgatttca tcgctgttga actgggcgca catttgattg
     8401 attttttctc gcccgatgcc atgttcccca ctgatgctgc cgccaacttc aacgcagagt
     8461 tcgaggatct tcccgcccag ctcttccgcg cgggcaaatt caccaggttc gttggcatcg
     8521 aaaaggatta acgggtgcat gttgccatct ccggcatgaa agacgttggc aacacgtaaa
     8581 tcatattgct gcgataaacg ggcaatgcct tccagtacgc caggcagggc gcgacgcggg
     8641 atggtgccat ccatgcagta gtaatccggg gagatacgtc ctaccgccgg gaacgcattt
     8701 ttgagaccgg cccagaaacg tacgcgctct gcttcgtcct gtgccaaacg gacgtcagtc
     8761 gcgcccgctt tcaacaagat gtagttaacc cgctcgcagt cttcctgtac gtcagactcc
     8821 acgccgtcca gttcgcataa caaaatcgct tcggcgtcga cgggataacc ggcatgaata
     8881 aaatcttccg ccgcgcggat cgacaggtta tccatcatct ccagcccgcc ggggataatg
     8941 ccattggcga tgatgtcacc aaccgcaagt ccggcttttt ctaccgagtc aaagctggct
     9001 aacagaaccc gcgccacggg cggcttcggc agcagtttta ccgtcacttc ggtggtcacg
     9061 ccgagcatac cttccgatcc ggtgaacagc gccagcaggt caaaaccagg tgaatccagc
     9121 gcgtccgatc ccagcgtcag cgcctcgccg tccagcgttt gcacttcaat tttcagcagg
     9181 ttatgtacgg tcagaccata tttcaggcag tggacgccac cggcattttc cgccacattg
     9241 ccgccaattg aacaggcgat ttgtgaggaa ggatccggcg cgtaatagag attatgcggc
     9301 gcaacggcct aggagatcgc caggttacat acgcccggct gcacgcgcgc gcggcgacca
     9361 acggggttaa tgtcgaggct ctctttaaag cgcgccatca ccaacaacac acctttttcc
     9421 agcggcagcg cgccaccaga aagcccggtg cctgcaccac gggtcaccac cggtacacgc
     9481 aggcgatggc agacagccag aatcgctgtc acctgttcca tttgcttagg cagaacaacc
     9541 agtaatggac gcgtgcgata cgcgctcaac ccgtcacact cgtaaggaat gatctcctca
     9601 tcggtatgca ggatctcaag tccagggaca tgctcacgca gtgccatcag taccgatgtg
     9661 cggtcgacat cgggtaaagc gccatcaaga cgctcttcgt acaagatgct catgagtagg
     9721 cttcgctttg ttgtgttgtg tggcagctga tttttgcgcg ctgcttctgt gaacagttat
     9781 taagcgggct tttcgttttc gtctatctct ttagctaccg gtcagaccat tttttttcca
     9841 gctctgtgac cttgtcttgg ttaacttaat gttaaattga tgtaacataa tcacttgcgt
     9901 gatgtgcgtg ttttgcgagt taagaacaga aaaattggtc ctacctgtgc acgaggtccg
     9961 ggaatgaaag atgaacgtcg ccctatttgc gaagtggttg cagagagtat cgaacggtta
    10021 attatcgacg gcgtactgaa ggtcggtcag ccgcttccct cggaacgtcg actgtgtgaa
    10081 aagctcggct tctcacgctc cgcactgcgt gaagggctga ccgtgctgcg cgggcgcggg
    10141 attattgaaa cggcgcaggg tcgcgattct catgtcgcac ggcttaatcg ggagcaggac
    10201 accagcccgc tgatccatct gttcagtacg cagccgcgaa cgctgtacga tctgctcgac
    10261 gttcgcgcat tactggaggg cgaatcggca aggctggcgg caacgctggg aacgcaggct
    10321 gattttgttg tgataacccg ctgttatgaa aaaatgctcg ccgccagtga gaaccacaaa
    10381 gagatttcgc tgatcgaaca tgcgcagttg gatcacgctt tccatctcgc catttgtcag
    10441 gcttctcaca atcaggtgct ggtgtttacg ctgcaatcat tgaccgatct gatgtttaat
    10501 tcagtgtttg ccagcgtaaa taatctctac catcgaccac agcaaaaaaa gcagatcgat
    10561 cgccagcatg cgcggatcta caacgcggtg ttgcagcggc tgccgcacgt cgctcagcgc
    10621 gcagcgcgcg atcatgtacg gaccgtgaaa aagaatctcc acgatatcga gctggaaggc
    10681 caccatttga ttcgctcggc ggtgccgctg gagatgaaca aggtgggaat gtgagcagag
    10741 ttttacccta tccccaaggc gaggggaccg cccctgcact taacaggatc tggtgttaaa
    10801 tctgttcatc tataccctct cccgaatgac cgcccctgcg ctaaacagga tctggtatta
    10861 aatttgcttt tcatacaatc ggtaacgctt gtacggctcc gccccaatgc gttccagcat
    10921 gttattcatg cctgtattgg tttcgaggat ccatgacatc tccagcgcat cgatcttccg
    10981 gcgggcaaac ggatcgcgta aggcttcaat caataacagc gcaatcaccg ggccgatgcg
    11041 gctgaactga tactcgtcgc gcacgcccat cagcggtact cgcgcagttc gcacaccgct
    11101 gactttcaaa cgccacagca attttgccca gccgaaggga aagagcgatc cgttcagatc
    11161 ggcaatcgcc tcgttgatgt taggcaagcc acaataaacg cgcagggtgc agaatcaatc
    11221 tcagcgatgt agatcatatc gtccggcacc agatatttaa gttggtcgcc catggtcgcg
    11281 aattcatgtt cggtaaacgg cacaaatccc cagttgtgct gccagccaga gttgaaaatc
    11341 tcacgcagga tctgcatctc ttcggcaaac cgctgacgat tgatgcagcg aatggtcacc
    11401 tttttgcgca cctgatccat cagttttttt agcgccggag agaaagtgag atcggttcgc
    11461 tgcatccacc acgccagtaa atcaatgcct ttgtgataac ccagttgttc aatatgcgcg
    11521 gcataccacg gtttgccgtg tggcatcatc gcacagggtg gtgtgtcaaa accttcaatc
    11581 agtaatccgc tttcctgatt gatattcagg ctgaaaggcc cgctgatctt acttgcacct
    11641 tgtgacttca accacgcttc cgctgcgcca aacaacgcgg caaaatcctg cggatcatca
    11701 atggcgtcaa tcatgccgaa atgaccggta tcttcaccgt aacgctcacg gtgcaaggta
    11761 tctatttgcg cggtaatacg cccaactatc tgccccgctt tttttgccac ccacgcctgc
    11821 cagatgatat ggtccgtccc cggatttttc gcagacaaat gctcgttgcg ttcaatgaat
    11881 aaagggggta tccagtttgg atcgtcggga taaagtgatg acggaaaagc gataaatgcc
    11941 ttaaggtcat ttttattaat tactttttca attttaatca tcagcagatc gcattccata
    12001 tctctgtgga taacaaataa ttaatggtga aaaacaggtt gcaacagcaa cgagcggcat
    12061 cttaataatc aactcaatga atacaaacga cgaataaata gtattaatta atgattatga
    12121 taaggtcaga ggctacacag aaacgcctca atgccttttg tcaatttaag gcgaccggac
    12181 aaggcttctt cttaatatat tcataaatta aatatcattc ataataaaaa caaaaaactt
    12241 aaactcatta tttaaatcaa tgttcatttt cacaccgatg aaaatactat ctcatttatc
    12301 ttttatgcct atataatcca ccgcattatt gtcattttat ttttgatatc caattttttc
    12361 gtggtgtata tgtctaataa aatctttacg cattccctac ctatgcgcta tgccgatttt
    12421 ccaacgctgg ttgatgcttt ggactacgcc gctctgagta gagccggaat gaatttttat
    12481 gacagacgtt gccaacttga agatcaactg gaatatcaga cattaaaaac gcgtgccgaa
    12541 gctggtgcga agcggttgtt atcgctgaac ctgaaaaaag gcgaacgcgt ggcgctgatt
    12601 gccgaaacga gtagcgggtt tgtagaggct ttttttgcct gtcagtatgc tggcttagtc
    12661 gccgtcccgt tggcgattcc aatgggcgtc ggtcagcggg attcctggag cgccaaactc
    12721 cagggtttac tggcaagttg ccagcccgca gccattatca ctggtgatga gtggttgcca
    12781 ctggtcaatg ccgcgacgca taacaacccc gaattacatg ttttaagcca cgcttggttt
    12841 aaggcattac cggaagccga tgttgcgctc cagcgtccag ttccgaacga tatcgcctac
    12901 ctccagtaca cctccggcag cacccgtttt ccccgtggcg tcattatcac ccatcgcgaa
    12961 gtgatggcta atctacgtgc tataagccac gatggcatta aattacgccc tagcgaccgc
    13021 tgcgtctcct ggcagccttt ctaccatgat atgggactgg tcggctttct cctgcccccc
    13081 gtcgccacgc aactttcagt agattatttg cgcactcagg attttgccat gcgtcctttg
    13141 caatggctta aattgatcag taaaaatcgc ggcaccgttt ccgttgcgcc gccgtttggc
    13201 tatgaattgt gccaacgccg cgtgaatgaa aaagatctcg ctgaactgga tctttcctgc
    13261 tggcgcgtcg ctggtattgg tgctgagccg atctccgcag aacaactcca tcaattcgct
    13321 gaatgtttcc gtcaggttaa ctttgacaat aaaactttca tgccgtgcta cggactggca
    13381 gaaaatgcgc tggctgtcag cttctctgat gaagcctccg gggttgtggt taacgaagtg
    13441 gatcgcgaca tcctcgaata tcagggtaaa gccgtcgcgc cgggtgcaga gacacgcgcc
    13501 gtatcgactt tcgtcaactg cggcaaagcg ttgccggaac atggtattga aatccgcaat
    13561 gaagcaggta tgccggtcgc ggaacgtgtg gtaggccata tttgcatctc cggtcccagt
    13621 ctgatgagcg gttactttgg cgaccaggtt tcgcaagacg agattgccgc gacgggctgg
    13681 ttagacaccg gcgacctcgg ttatctgctg gacggttatc tgtatgtcac cggacgcatt
    13741 aaagatctga ttattattcg tggccgtaat atctggccgc aggatattga atatatagcg
    13801 gaacaggaac cggaaattca ttctggcgat gcgattgctt ttgttaccgc ccaggaaaaa
    13861 atcattttgc agatccagtg tcggatcagc gacgaagaac gtcgcgggca gcttatccac
    13921 gcgctggcgg cacggatcca aagcgaattt ggcgtgaccg cggctatcga tctgttgccg
    13981 ccccacagta ttccccgaac gtcctccggc aagcctgccc gtgcggaagc gaaaaaacgt
    14041 tatcagaagg cttatgctgc cagtcttaat gtgcaggaat ccctggcata aatcaaactg
    14101 tcgcggtgac gggcgctacc gggtttatcg gtaaatatat tatcgataac ctgctcgccc
    14161 gcggctttca cgttcgcgca ttgacgcgta ctgcccgcgc tcacgtcaat gataatctca
    14221 cctgggtgcg cggttcgctg gaagatactc attcacttag cgagctggtt gccggagcca
    14281 gcgtagtggt ccattgcgcc gggcaagtgc gcgggcacaa agaagagatt ttcacccgct
    14341 gtaacgttga cggcagcctg cgcctgatgc aagcagcaaa agagagcggc ttttgccaac
    14401 gttttctgtt tatctcttcg ctggcggcgc gccatcccga gctctcctgg tatgcaaaat
    14461 ccaaatacgt cgccgagcaa cgactggcag caatggcgga cgaaattacg ctgggcgttt
    14521 ttcgcccgac agccgtgtat ggtcccggcg ataaagagtt aaaaccgctg tttgactgga
    14581 tgctacgcgg cctgctgcca cgacttggtg cgccagatac acaactctct tttcttcacg
    14641 tcaccgattt tgcgcaagca gtgggtcagt ggttaagcgc cgaaactgta cagacgcaaa
    14701 cctatgaatt atgcgatggc gtcgccggtg gctatgactg gcaacgcgta cgacagcttg
    14761 ccgccaaagc ccgttgtggt tccgtgcgaa tggttgacat tcctctgccg gtactcacag
    14821 gccttgcgga tatcagtacc gcgttgagtc gcctggcagg taaagaacct atgctgaccc
    14881 gctcgaaaat tcgtgaatta acccacgccg actggtcggc aagtaataac cgtatttctg
    14941 aagatatcaa ttggtttccc gggattagcc tggaacacgc attacgcaac gggctatttt
    15001 gattgtcttg ttttaaaggt gtgttaacgt ttctatggta aatcgtgaaa tagtaatgga
    15061 ttacatcctt tcctgtttac aggatttggt cgaaaacggt gtggaaatta agcccgacag
    15121 cgatctcgtc aacgatcttg gcctggagtc gatcaaagtg atggatctct taatgatgct
    15181 ggaagacaga ttcgatattt ccattcctat taatattctg ctggatgtca aaacccctgc
    15241 gcaattaatg gaaactctac ttccatggct ggagaataaa taatggggct atacgataaa
    15301 tatgcgcgcc ttgctggcga gcgtctgcaa tttagtgaca acgggttaac accgtttggc
    15361 acctgtattg atgaagtgta ttccgccacc gaaggccgga tcggcaataa aaaagtgatt
    15421 ctggccggga ccaataatta tctcggttta acctttaatc atgatgccat cagcgaaggt
    15481 caggccgcgt tagcggccca aggcaccggc accaccggtt ctcgtatggc aaacggcagt
    15541 tatgcgccgc acctggcgtt agagaaagag attgccgagt ttttcaatcg ccctaccgct
    15601 atcgtttttc aaccggttat accgctaatt taggcgtcat cagcgcactt gccgaccata
    15661 acgccgtggt gttactggat gccgacagcc acgccagtat ttatgacgtc tgttctctcg
    15721 gtggcgcgga aattattcgt ttccgccata acgatgctaa agatctggaa cggcgcatgg
    15781 ttcgcctcgg tgaacgggcg aaagaggcga ttatcatcgt tgagggcatc tacagcatgc
    15841 tcggtgacgt tgcgccgctg gcggaaattg ttgatatcaa acgtcgtctc ggtggttatt
    15901 tgattgtcga tgaagcgcat tcgtttggcg tgttaggcgc aacggggcgc ggactggctg
    15961 aagctgtcgg cgttgaagat gatgtggata ttatcgttgg tactttcagt aaaagtctgg
    16021 cctcgattgg cggttttgct gtcgtttcgg aagcgatgga agtgctgcgc tacggcagcc
    16081 gcccgtatat ttttaccgcc tctccttctc cgtcctgcat tgcgaccgtg cgctcgtcgt
    16141 taagaactat tgccacacaa ccagagctgc ggcaaaagct gatggataac gccaaccatc
    16201 tgtatgacgg tctgcaaaaa ctgggttatg agttaagttc acatattagc ccggtagtgc
    16261 cagtaataat cggttcgaaa gaggaaggtt tacgcatctg gcgcgagctt atctcgccgg
    16321 gcgtgtatgt gaatcttatc ctgccgcctg ccgcgcctgc aggcatcacg ctgctgcgct
    16381 gtagcgtaaa tgctgcccac agtcgtgagc aaatcgatgc catcattcag gcatttgcta
    16441 ctctgaagca gtaatgtgag cccgccgtgg tgtttgcgcc acggcgggtt taatgttgtc
    16501 tgtcatacca atacaccagg ctcaacgcca ccaacacaaa cgctaccggt aaaccgagcg
    16561 cggtaacagg tgcgcttaac gcaaatagca atcccagatt aaccatgatt tgatagctga
    16621 tccaggtgag taagcccacg atgacgccta cagcgagacg gctacccatc ccaggcgaac
    16681 gcggggcact aaaggtaaac ggcacagcca gcaaaatcat cgccagggtc aggatgggtt
    16741 gccccagttt ttcccacagt gcaaggtggt attcgctgct gggttgcccg gtttcctgca
    16801 aataatggat gtaatgatta agctgacgga cagaaaaagt attccccggc attgataact
    16861 cttcaagatt catactggtg aaggccgatt gccaggcaag attatctaat gtttccagcg
    16921 tttctttgcc attcagccat tttttatgat tcacaccatg caggacccac gttttgtcgt
    16981 cttcaatggt ggcactgcgt gcataaatgt aggattcaag cgagagatcg tcgcgataat
    17041 gaaatatctc cacgcccacc ggctggccct gctcgttcag cgatttcacc gtcacaaatt
    17101 cattaccacg cctggcccac agcatattgc cggtaatgtc atcgtcctcc cccaacgcgg
    17161 tggcggtcga tttgatttgc aacgcctgtt gctgcaatgg cgacgccacc cactcatcta
    17221 tcgcgcctaa cgaaacagtc cacaatatcc cggcaaccag cgccaccagt gcaatacgga
    17281 agatagaaaa ccccgtgctg cgaatggcgg taagctcact gtttttcgat aactgcccca
    17341 gtccaacaat accacccaat aacgcgataa acggcgaaag ttcgaccagt gtgcgcggta
    17401 aggtcattag caccaccagc accgcctgag tccagcgata accgcccggg ctgacaccat
    17461 ccagttcgtt aatcaggttg aaggtggtaa aaagcggcag caatagccct gcagcggcgg
    17521 caaaaccgag aaagagatga cggattaaat agcgactgaa aacattcatc gggaaaattt
    17581 ctgcagcaaa gagaagtcac gtgcgaccag tatcagcagg cccattagca tgagtccggg
    17641 cactaaccac aaaccaggaa tgaggggaat cgcgccgtta gccaccagtg tacggcagac
    17701 gtcgccgcca taaaaaatgg caataaacag caacgtcagt ggcaataacg ttgaaaatcg
    17761 cccttgccgc ggttttaccc gacttaatga aatggccagc agcgccatca acaatgcggt
    17821 caggccacgg ctttgtcgcc attgcagttc ggcatgatcg gcaggaaagg cggagcgcgc
    17881 cagctccgtg accgatttcg ctttgcgttt gacgttcggg ctttgatcca gcggattcag
    17941 atgtaattgc agattacggt agatctgttc gttgtcgtca cgcccctgat gatcgagaag
    18001 ataggcagtg ccgttgtgca acataacggt cggtttctcg ggtgatgggt caaccacatc
    18061 aaccgaaccg gcgcggaaaa tacgggttcg attggcagta gaagtataaa tcagcgcgtc
    18121 agtcaggcga ttattatcct gatcaaccgt ctgcgaaagg atcattcgtc cgttatcgtt
    18181 agtgttaaat ttctttgccc gcaactggcg aacatccagc tccgactgcg actgctgctc
    18241 cagttgataa atctgcgcat aagcccacgg acgaccatac atcgacagaa gggtgacaaa
    18301 aatacttagc ggtatcgcca gataaagaac ggctttgtac aagcgtcccg gactgccccc
    18361 cgccgcagag atagcggtaa tttccgagtc ggtatacatc tgccctagcg tcacgccaac
    18421 tgacacatac agaccaacag gtaacaacat ctccagtgca atcagtactt tgtagaaaac
    18481 gatatccagc acaacatcaa gagctaatgt cccatttgcc gcttcggtca gataacgctg
    18541 tgcggagtag ctggcgaaaa taaagatcag aaaaccgacg ataatcagca ccagacggcg
    18601 cgtaccacgc atgatgtagt gttcaaccag cttcatctca ctaacgccct caatcctttg
    18661 agttttttat tcattgatca gtttctgcgc caacgcaatg acatgatcta cctcttccgg
    18721 cgtcaggctg ccgtcctttg cccactgaat ttgcccggct ttattcagca ccagaatggt
    18781 tgaactctgc tccggcaagc gccaggcaac gcgtccctgt ccattgccgt caataacgaa
    18841 ttgcgcccac ggatagcggc gtttattttt ttcgattttg ccaacaacaa aatagccagt
    18901 accaaagatg gcatcatcgg tgttcacaat ggtggtgggc tgaaaacgat cctgcggaaa
    18961 gtttgccgct tcaaccgctt taattaacag tgagttcttt ttcttcgccg acttacgccc
    19021 ggcgatatac tgaataatgc ggacttttcc cgctaattgt gtgctgttcc acttttggta
    19081 gtgaatttcg tcgtcgctat tcagaagcag ctcgccgcct tcctggatat aaacaggggt
    19141 aaccgttttg ccagtgacga aattgtgtgc ctgcacaccc ggggctaaga gcatgataat
    19201 caacgcaata atcaaacgag atgacatcat tgctccggct atagcggaaa acgcgaaatc
    19261 agcagctcta agcgctgaca aatatcgcta tccagcgcca acggtgcata atacgactgg
    19321 cggttttcca tatcatccgc caccatgtcg tcgtgtttat ctcccatcag gccattaatg
    19381 gcggcgagga aacgggaaat ctgtaacgcc tctttatttt caggatcaag cgatcttaag
    19441 gtgtcgtaat aagaaacctg ctggctgatg atttttcgca catcatgaaa ttccctgccg
    19501 gttagtcgcg gtttggcaat aatctctttc agggtatgca tttgcttttg ccgataggca
    19561 ataaaactgc ttacgctttc taactggcta tggcgtaatg aatagttcac caacgaccag
    19621 accggagagc ttaaataaat ccgtaacagg ttgccgtaat acgacacaat atttttctta
    19681 ccgttacgaa agccgtcctg aaaacgcccc agaaaaacgg tcgttttgcc gaataaaaat
    19741 ccggcctgat gcttctttaa atacagccgt tgcgcaaaac gcaggtgctt gtagcgcgct
    19801 cgcatgtatt taaattgttt gcgctcatca gcagtcagct catcgccttt ggcttgtttg
    19861 cgaactaaac gcagtaattc ctgacgatca acgccattga gccagaattg caggcataac
    19921 gcgtaattat catgaatgat ttcggtggta caacttagtg caatagtcgg cggcaaacta
    19981 acctgtgcat caacgacatc atcttcttca atcgcaacaa atagacgctc gctcgcctca
    20041 agcgggaaat attttctcat catcaggatt tctctccggc tgcgttataa agcaaggaat
    20101 ggcgctgaaa aaatgccttt aaggttggaa ttaacgagag cgccacgtcc agacattgcc
    20161 caccgaagta agcaaacgct gctcccagca caccaaacag atgacagaac atgaacagca
    20221 atacgatgta gccgattgag gcgatggttt gcgccacgag aaaagcgcgc tgttttcccg
    20281 ccatcaatag caatgactcc tgcgggaagc ccagcatcga aatcacaatt gcgcccagca
    20341 tcacctgaat caggtcatac gcttcgagat acttaacgcc aaacaccagc gaaatgagcg
    20401 gcttgccgac gatcaacacc gcgagcgcca ccagaatacc gattccgccc gccagtaaac
    20461 cggacttcac acctaacaac cacggtctgg tggtgcgcgg atctaaacgc atcacctctg
    20521 ggtaaaaact tttacccagc aaacccgctg gcgttccggc ggcgtcgaag aatgtcatgg
    20581 cgattttaaa taacccggcg gcagcgggtc ctaacacgat ccccaccaac actgtgctgc
    20641 acgagttacg cgccgaccag atggagtggg caatgttggt tgaccagaca aaactccacg
    20701 cgcctttaat gtgtcgggca gactcaaaca gattcaattt gaaggcgtta tggatatttc
    20761 tacggcgtaa ttcgcgcgcg gcaaaccacc agtacatggt gccgccaacc agattcgaca
    20821 cgtaccaagc aataacaaaa cccacaaaac caaagtcaaa ataccaggct gcgacgctcc
    20881 ccgctgcgcg cagaaaaggt ttcgtcgcct gctgtacagc aatcaaatca aagcgatcta
    20941 ccgcacgcag aatgccggtc ggcgtggagg aagccattga aggaatgagc gtgcaataca
    21001 gcgccgccag ccaaaagctt tggtcatcca gacctaatga atgggaaagg aatggcagta
    21061 aggcaatgcc gccgacaatc gccaccgcgc cgctgacgat atccagcgag aaggaaaatg
    21121 agacgacatt gcgaaattgc tgcggattat tgttggtcaa ggctggcgtt ccgtactgaa
    21181 ccaccagctg ccatgtctga aacttaataa aatcgctgat cgacttggcg tacgattgca
    21241 caatcaccag tacaccaaac atggcgggcg tcatcccttt accggcacac gagagcgcca
    21301 acagccccag caaggcgctc tcgacattac tggagcctaa ccaggcgcta ttgcgaataa
    21361 tggtgcgaaa cgcgccatct gcaaaccaat gtttgatgtt aaaacccgcc aattcagcct
    21421 gaccttatcg ttgatagtaa aaaatatccc gccagcctta agttaaactt cggcggtcag
    21481 aaacgatggc aaccagagaa accgccttct gtacctgttc cagcacttcg ctgtagggcg
    21541 ctctggaatc aatctcaaga atttttgtgc cgttatagcc aatcgtcgac atgacgccga
    21601 ttttgtcctg cagctcggca tactcatggt caggcttgcg ggagatggca gtatcaatat
    21661 caatccccaa acgaatgatt aattcaggac ggtattgcgc cattttttgg tataaactcc
    21721 gttcgcgctg cgccagaaac atgctgattt tcccggtcgc acgttcgacg ccgatccccg
    21781 gtccgtcata ataaaagccc gaaatttcag cctgcgggaa gcgatcgctg accaccagaa
    21841 cgccactttg cgccagtcgc tgaaccttgc gtagattcgc cattcttcgc agcgagaagc
    21901 agtacataat caccgccgcc catagcgccg gagatttggt tttcatgctt tgggttttcg
    21961 aggatttggc cgccaatcgc cgttccagcc agacgccaac caacggcaat cgtttgattt
    22021 tgtcgccgtc ttcgccggag agcagcccca gatagcggcg ctcggtttgc cagtgttgtt
    22081 gcagcgattt caccaggtcg gtggtcagcg tggatttacc ggtgccatca cacccgacca
    22141 ccgcaatcag ccccggaatg tagttgggct gcggcgcggt tgtactatta acagtttgtg
    22201 tttgtagtgc atccattcgt taaatcatcc cctatccatt ttggtgatca ggaaagcgcg
    22261 gcgtgaattg cgcgtagtga cgcttgcata atttcatcgg ctggttgccg accatccagt
    22321 tcaaggatct tggcgccatt gaatgtcagt tgcggcgtaa cggcgatttt ttcctgtaac
    22381 gctgccagtt ggtggtcagg tttacgcgca aacgcggttt gttcatcaat gccaagacga
    22441 atcaacaata cgggtaaata agacgccatc cattggtaca gcttcaactc gcgctgcctt
    22501 aacattttta tccaaccgtt accgcccgtg gtttttgcca attgcgggcc atcaaagcga
    22561 aaacccggca cttcaacctg cgggtagcgg tcggtgatga gcagatagcc ttgctggctt
    22621 ttacacaaca ttttgcgaaa cttgtacgcc cgccagcagg aaagcagata gataaccagc
    22681 gcagtaatat tgccaggcgg agttaagggc ttttcgtgta catgtgccgc tttacttcgc
    22741 agataacacc cacaaggtgc gccaataaca gggagctgtg aaatccattc gccaattcgc
    22801 ccggacgatt gcccgagata aatgtgttct gttggcattc ttgctgccag ttcatttacc
    22861 aggcttgccg tgagggtcga tttacctgaa ccatcacatc ctacaatggc aataacacgc
    22921 actggcgtgg aattaattat tgacatatta cgttgattca cgaaaaaccc ggcagtaaat
    22981 aatgtattga atattacgtg gtccgtgctc agaatatccg ttcaacctta ttgtgacaat
    23041 gactaattgt gacattgacc tgaattacgc tttcataaaa acatattaac caaataaata
    23101 tttttaatgg atatttaaat taaaggatat attcatgcag tcaataacac ctccattaat
    23161 tgccgttatt ggtagcgatg cttcaggcaa gtcaacggtg tgtgaacatc ttattaccgt
    23221 tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga aaacaggccg gaaatgtcgg
    23281 tcgtgcagtg acaaaattac ctttgatggg aaaatcctta cataaaacaa ttgaacgaaa
    23341 tcaggtgaaa acagcaaaaa aattgcctgg accagttccg gcgctggtaa ttacagcgtt
    23401 tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc tgtcgtcgtc gcgggttaat
    23461 tgttctaacc gaccgttatc ctcaggacca aattcctggc gcttacgatg gtacggtgtt
    23521 cccacctaac gttgaaggtg gtcgttttgt ctcatggctg gcaagccagg aacgtaaagc
    23581 gtttcactgg atggcgagcc ataagcctga tctggtcatc aaactcaatg ttgaccttga
    23641 agttgcctgt gcacgtaaac ccgaccataa acgggaatcg ctggcgagga agattgccat
    23701 aacgccacag ttaacctttg gtggtgcaca actggttgat atcgatgcca atcagccact
    23761 ggaacaggtg ttggttgatg cagaaaaagc gattacggat tttatgaccg cgcgtggtta
    23821 tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg gcattttcag gaaggttaaa
    23881 tcaactgcaa tgctatccag tacagcccac cagaaagaaa aattgccgcc ggtaaagtaa
    23941 acacccacgc catcaggatg ctggttaccg ttttacgctg taacccaccg ccgtccacca
    24001 ccatcgtccc tgctaccgca gacgagagga cgtgtgttgt ggagacaggc atcccaatat
    24061 aactggcaag gccgatagac actgccgccg tcatttgtgc cgccatgcct tgcgcatacg
    24121 tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc tacacgacgc cagccaatca
    24181 tggtgccaat gccgagcgcc agtgctaccg ccatgatgat ccacaccgga gcgtactcaa
    24241 tggtgcttaa catatcgctg cgcagttttt tcagcaggtt ctggtcttct ttactgacgc
    24301 ctggcagttt cgccagcttc gcggaggtat cggagatgca cagcataatg cggcgcagct
    24361 ggctgcgctg actcacgctt aacggctcgt aactttccat attgcctggc agcatcgttt
    24421 taacgcgcgc aatcgcatca aaggtatttg ccggatgaca gtgaaactct gttacttgcg
    24481 tgccatcagt cgatgctgca ggcaatggag gttccatcgc aatcaacttc tgcggcagtt
    24541 caggatgctg ttgcaggtag tgttcgaagt tggtaacggc atcgcgggta cgggtaattt
    24601 catagccgga cgcattcata ttgacgacga agccagcagg ggcaatcccc accagtacca
    24661 gcattaccag gccgatccct ttctgcccgt cgttcgcgcc gtgcgaaaac gccacgcccg
    24721 cagcggaaac aatcagcgca atacgcgtcc agaatggcgg tttacgtttg ccctttttct
    24781 ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt tttcgtccca ctccagaaac
    24841 ggcgcagcag gaaaatcaga ccacccgcaa tgaccaggcc gacgataggg gaaacaatca
    24901 gcgaggagaa aattttggtt acttcacgca ggtttaacgc atccatcacc gatgagccgg
    24961 ttaacagcgc gttggttaaa ccgatgccga taatcgcacc aatcaaggtg tgcgaactgg
    25021 aggccggtaa accaaagaac cacgttccca ggttccagat aatcgccgcc agcagcatgg
    25081 aaaagaccat cgccaaaccg tgagttgacc ccatattcag caacaaatcg attggtaaca
    25141 tatggacaat ggcataggca acgctaagtc cgcccaataa cacgccaaaa aagttaaaaa
    25201 atgccgccat caccacagca agttgtggct gcatggcacg agtataaata acggttgcca
    25261 ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta gaacaacaca aatgccagag
    25321 caagtaataa caaaagcccc gtatatatat caaggccaac aaataaattt agcatatatt
    25381 agattacgcc attttgaatt tacgaacgga cgcattatca gtgactttaa cggcatgggc
    25441 aaagtgaaat atcatttttt aacgataaaa agagtgttat ttcttgtgta tcagacaata
    25501 agcattcatt caaaaccttt agaaataatc aaaacacagg tattccactg gttaatatat
    25561 tcacatatga aatgaataaa tattggaata tataaatatt gaatattttg attaatcccc
    25621 gtactaatta ttcttcataa tcagtacggg gttgctacaa catgtattac tttttcacca
    25681 caattaacgg ttcaatatca ctctcttttt tgatgaccag tgattcatca ccgcgcaaac
    25741 acgtcccacc gtagttgccg ccaacggtga aggtacatac ctgaatgtat ttaccgtcca
    25801 ctttcggcaa acaccacagt tgctgataga tgtttttctg ctcggcaaat ttaccgctgg
    25861 ttttgtccag cacctcttca tgatggctga ccaggtcgat attgctacca cagcgaccgg
    25921 cgatcggttt cactgcgtaa cctgttttca ccaattcatc attaacagtg aaatcggtat
    25981 ccagcaggta acgatggtgc gggaacagcg accagaggat cggcagaatc gctttgttgc
    26041 cggggatcac cgtccacagc ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga
    26101 taagacgcac ttcgttttgc ggatgaccgg tacggattgg caccgcagca aactcacggt
    26161 cgctaacttc acgaatctga tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt
    26221 taaccagtcg cccttcccca tcaatcagtt gcccggcagc atcccagccc agttcatcca
    26281 gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg
    26341 cgtgatagtt ttcctcgata tctttgtcct gcatgatatg gacaaacgga cgtgcacgac
    26401 tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc cgccggattg aagccgttgc
    26461 ctttatagcc ctgctccgcc caacgttcga ggatcaagcc cgcttcggta tgacaggagg
    26521 cggagtcggc gttgtattcg taaaccttca ggccacgctc atccatgcag aaatccatac
    26581 gaccagtgat catatggtga cggcgacgct gccaggagag acgcaaacgt ggccagagga
    26641 ttttcggaat gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt
    26701 gaagatacat cagatgcagc tcgttggtgg ctttaattag ctcctgctcg gcactctcgg
    26761 taatggtgta gtaatgatac ggatcctgat tgatcacctg accgttggcc tgcacatagg
    26821 cgttttgtag cggatctttt tcatccagcc atttaccgtc aaactggcct ttgttttcca
    26881 gacgcgctcc actgattttc agcagctcgc ctgcaatttc cggctgcggt aagctgtatt
    26941 cagtatcttc cgtctggatc atccagccca gaatggtggt gtcatcaaaa gtgtctttaa
    27001 gggtatagca gccgttttcg accaccatct ccagctcgcg cgtccactgt tgcccttgcg
    27061 gcaacgggga atgaatcacg ttctgttccg caatacggac tttgttgcca tgcaattggg
    27121 taatgatggc gacatggcca gtgtctttaa attcaccgcc tttatcccag ataagaagcg
    27181 cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt
    27241 cattaaccac ttctcgcagg aagcgcagcg agaaaatctc ccacgccata cccacgtcag
    27301 taaagaccac accgtaattc agaaagagaa aacggcgagc aaattcaacg cattgccact
    27361 tgtggcccat atattcgtcg tcgatatagc tacggaatac ggcgtcatct tcgtattcct
    27421 gcggatcgag agaactgtaa tctgaagagt agattgctac cccacctggg gcgtagccca
    27481 ataatgtccc gaacggggca tcctggctgg tcgttccttt gctcatcact ttacctttaa
    27541 caatacaacc taagcagttg gcgtaattgt tgtgctctga ttacctgctc agcgacatta
    27601 accggacaga ggtcagacta atcatacacc tcatcgcagc ggctggcgca aagggttaaa
    27661 aaattcacat tctgtacagc aagtgacctg ctacactgct tcaacactac cactcagaag
    27721 gcaactcact atgacagaca atacttatca gcccgcgaaa gtctggacgt gggataaatc
    27781 cgctggcggc gcgttcgcca atatcaatcg cccgttttct ggtccgacgc atgaaaaaac
    27841 gctgcccgtt ggcaaacatc cattgcaact ttattcgctg ggaacgccga acggtcagaa
    27901 agtaacgatt atgcttgagg agctactggc gctgggcgtt actggtgcag agtacgacgc
    27961 ctggctgatt cgtattggcg atggcgatca attctccagc ggctttgtcg aagtgaaccc
    28021 aaactcgaag atcccggcgc tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga
    28081 atctggttcg atcctgcttt atctggcgga gaaatttggc tacttcctgc cgcaggatct
    28141 ggcaaaacgg actgaaacgt tgaactggct gttctggtta cagggtgccg cgccgttcct
    28201 cggcggtggt tttggtcact tttaccatta cgcgccggtg aaaattgagt acgccatcaa
    28261 ccgctttacc atggaagcca agcgcctgct cgacgtgctg gataagcaac tggcgcagca
    28321 taagtttgtt gcgggcgatg agtacaccat tgcggatatg gcgatttggc cgtggtttgg
    28381 caacgtggtg ctcggcggcg tgtatgatgc cgcagagttt ctggatgcgg gaagttataa
    28441 acatgtgcaa cgctgggcga aagaagtggg cgaacgtccg gcagtgaaac gtgggcgtat
    28501 tgttaaccgc accaacgggc cgctgaacga gcagttgcat gagcgccatg acgccagtga
    28561 ttttgagaca aatacagaag ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag
    28621 ccatccaggc cggataaggc gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc
    28681 gacgctgccg cgtcttatca ggcctacaaa agctaatcat cacgcgctgg taatgtcgta
    28741 atccatttgg cgcagtgcgt ctaatgtggc tttggcttca tcttcgtcga tgatgctcat
    28801 ggcaaatccg acgtgtacca gcacccactg gcccagcaga tcggcagggt taccttcgca
    28861 aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg caagctggtg
    28921 aatatcttca ccgacagcca gcacctggcc tggaacgcca atacacattg ttaactccgg
    28981 ttatttcgat tgccggacaa acgcgaaaaa gaacctttag cgggtgttcc ctctcaccct
    29041 aaccctctcc ccggaggggc gaggggaccg tccgtgcgct ttttaggcgt tgtcctcagt
    29101 cttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
    29161 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
    29221 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
    29281 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
    29341 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
    29401 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc cctcctgcgg
    29461 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattggtgac tggcaacgaa
    29521 aggatcattc gtgcgcagtc atccgtcagg cgttggcctt cttcaatcga catgctgtgc
    29581 gaaagcggcg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
    29641 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
    29701 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
    29761 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
    29821 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
    29881 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
    29941 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
    30001 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
    30061 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
    30121 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
    30181 caggatcatc atcgttcccg gagtgttctt tttcgacaca atggcatccg caataatcag
    30241 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
    30301 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacgccgat
    30361 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
    30421 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
    30481 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc ggcaaccggt
    30541 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
    30601 ttccaggttg atggaacaac cgcctggtag ttgctgatga taccgtcttt aataaccatc
    30661 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
    30721 gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
    30781 gcactgtatt ggttttgcag gatatcctgc aattcacagc agtgaacggt acgaccaata
    30841 atacggccca gcgtggagtg cagctgtgcc acttccagcg tgttgccagt cagtttctga
    30901 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
    30961 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
    31021 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
    31081 cacggcgcct gtggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
    31141 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
    31201 tcaatgtagc ctcccgggaa caggaagctg ccgtttttgc tgtcggtcgg gaattccggc
    31261 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggcg
    31321 ataaccgcgg tatcaacctt ataaacctgc tcaacaaagt cgctcagttt gtcgatgaaa
    31381 gacttgatgt acatcaggcg ctcaaggttc aacacgccca acccgtcgag gttgattggg
    31441 ttcgcgacac cacccacagc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
    31501 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
    31561 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
    31621 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
    31681 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
    31741 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
    31801 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
    31861 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
    31921 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
    31981 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
    32041 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
    32101 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
    32161 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
    32221 caatagaaat caacagttct tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga
    32281 atgccaccaa cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
    32341 acaggaacag catacgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
    32401 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
    32461 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cccgatgaaa atcgccagca
    32521 acacactgat ggtgttggtc agcttgacga acaggctctt ttcatccgga ccgttgccac
    32581 gcagacccgc ctgcaccagt gaaccttcaa agatgacaat cgagaagccc atgatgaacg
    32641 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccagagcgga tgcaccttgt
    32701 agcccgccga gatcatcagc gaccccattg aagactggtg catggtcggc agcagcgcac
    32761 cgagcgcgat gatgaagaac atcaccttgt tcagacgttg tagcgacacc ttccagccca
    32821 gacgttcaaa cagtgccgga gcaaactcca gagccatcac gccgatgtag atggtcatac
    32881 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
    32941 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
    33001 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
    33061 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa
    33121 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
    33181 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
    33241 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
    33301 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
    33361 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
    33421 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
    33481 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
    33541 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
    33601 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
    33661 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
    33721 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
    33781 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
    33841 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
    33901 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
    33961 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
    34021 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
    34081 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
    34141 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
    34201 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
    34261 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
    34321 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
    34381 cgcctgcaga gacgttgccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
    34441 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata cctttttcgt
    34501 tacagccata gcacgggtga ccaatcgcca ccggccacac accgccaaca tcgcagaatt
    34561 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaagcacc
    34621 agccttcgcg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
    34681 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cggtttttgt
    34741 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
    34801 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
    34861 agctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcggagcagg
    34921 aaccaatagc gataatggct gctgagcctt ccgccgcttt gcggatgtga tccacaatcg
    34981 gctcaccggc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacca
    35041 ctaacacata ctgccctttg tacttctcga gagcgttatg tttgttctct tcgacctgat
    35101 gaccgaaggc ggcggaaagc acttcgtgat attccagaga gatagtctcc agcacgaggt
    35161 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
    35221 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
    35281 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
    35341 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
    35401 ttgcgagaac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
    35461 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
    35521 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
    35581 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    35641 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    35701 ggcgctaagc tggcgatctc cggtcttttc gtacatcctg tgtgagtagc ctaatacaaa
    35761 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    35821 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcatta
    35881 tatatcgcca tatataacga tattttaggc aaagaacgac ttttgcgcac gctatttttt
    35941 catacttgtt taagacgtaa tcccgtaaga gataaaccta tcaggagaga tatttatcga
    36001 accatttcag tgtcctttgc caggcaagat cggcggcaga tttgtcataa cggggcgtgg
    36061 aatcattatg gaatccgtga ttaacccccg gatagatata agcctcataa accttattat
    36121 tgtctttcaa cgccgcctcg taaggaggcc agccctcgtt gattcgggtg tccagttccg
    36181 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    36241 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacgc
    36301 caccgccata gcaaaatccg gtaataccca ctttacctgt cgcttgcgga tagcgttgca
    36361 taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca
    36421 gctcacgacc tttatcatca tttcccggat aacctccaac ggaacttaat ccgtcaggtg
    36481 ccagggcgat ataccccgcc ttcgccactc gccgtgccac atcttcgata tacggattca
    36541 gtccacgatt ctcatgcacc acccccacgg ctggcgtttt gccgctcatt tttgcgggtt
    36601 tcaccagata accccgtacc tcgccgtgac catttggcga aggatacgtg atgtactcag
    36661 caacaatctc cgggtcggta aactctacct gagtcgccag cgcataattt ggcttgagca
    36721 aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca aggttgagga
    36781 actcacgttt cgaaattttc ccgtgaacgt aatagtcgta gtaatccaac aactcttgtg
    36841 ggaaatcttt ggcggttaaa cgcggcatcg ttgcattcct caactggtgt ttttttaagc
    36901 aaagcataag cgcgtgtttt tgcccagttt ttcgtcactc tgtgagccag actacgggat
    36961 acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacggt ggaaaggaga
    37021 ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcagtacc gctattgcgg
    37081 aaaaagtggt ttacgcctgc ccgcattatc gctcggttta tggcacaatt tcggtcacgt
    37141 taacgcgctg gaatcacagc gtgcaatcct gcgtaaagcg tttgatttag gcattacgca
    37201 ctttgattta gccaacaatt acgggccgcc tccaggaagc gcagaagaga actttggtcg
    37261 cctgctgcgc gaggattttg ctgcttatcg cgatgaactg attatctcta ccaaggctgg
    37321 ctacgatatg tggcccggcc cgtacggctc tggcggttca cgtaaatacc tgctcgccag
    37381 cctcgaccaa agcctgaagc gtatggggct tgagtatgtc gatatctttt actctcatcg
    37441 cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg cggtacaaag
    37501 cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc aaaaaatggt
    37561 cgagttgctg cacgagtgga aaattccgct tttaattcat caaccttcgt acaatttact
    37621 gaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg gcgtgggctg
    37681 tattgccttt actcctctgg ctcagggatt gctgaccgga aaatatctca acggcattcc
    37741 gcaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct
    37801 taccgaagcc aacctcaaca gcctgcgctt attgaatgaa atggcacagc agcgtggaca
    37861 atcaatggcg caaatggcgt taagctggtt gctgaaagat gatcgcgtga cgtcggtatt
    37921 gattggtgcc agccgcgcgg agcaactaga ggagaacgtg caggcgctga ataatctgac
    37981 atttagcacc gaggagctgg cgcagattga tcagcatatc gccgatggcg agctgaatct
    38041 gtggcaggcg tcgtccgata aatgacctgt taataacggg cgcgcgaacc gcgcccgaat
    38101 cagatcagtg attatgacga gtcagtcggt caagatagcc catcacaaat gcagaaagca
    38161 caaacgtcag atggataatg acgtaccaca tcagtttgtt atccgggaca tttttcgcat
    38221 ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct gctactttgt
    38281 ttttcagcga cgttgcgtcc attttcccca gccacttcag cttctctttg ttctcggaga
    38341 tatccagctg tgagacgaaa ttctcataac cggaaaacat caccatcacc agcaaaccgc
    38401 caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat tccgccatcg
    38461 agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc agggcaacta
    38521 acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca tacattgcat
    38581 tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgggcaac agtatatcgc
    38641 aattgagtaa acagcacgca acagcaaatc aacatatacg ttaaaccttt gtcgcataat
    38701 tctgaggttt agggatcttc ctggttccct tatcaacgca tcgagagcgt ctacccttaa
    38761 aggacgttga acctgaaggg agaaaaacga tgtctcattt aaaagacccg accacgcagt
    38821 attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga
    38881 caccgatacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg aaagatcgta
    38941 aagcactggt gacagggggc gattccggaa taggtcgcgc tgccgccatc gcttacgcgc
    39001 gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg
    39061 tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg
    39121 atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc gggctggata
    39181 ttatggcgct ggtcgccggg aaacaggttg ccattccgga tattgcagac ctcaccagcg
    39241 aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta acccaggaag
    39301 cgatccccct gctaccgaaa ggtgcaagtg tcatcaccac ttcgtcaatc caggcatacc
    39361 agccaagtcc gcatttactg gactatgcgg ctacgaaggc ggcgattctg aactacagcc
    39421 gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc
    39481 ctatctggac ggcactgcaa atttccggcg gacaaacgca ggataagatc ccgcagtttg
    39541 gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga actggcccct gtatatgttt
    39601 atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg
    39661 agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat tgtgaattca
    39721 ctcttacctg atgtggcgaa aacgccttat ccgcctacaa acgcatgaag attcaacatg
    39781 ttgcaataga ttttgtaggc ctgataagcg tagcgcatca ggcattgagc acctaatgcc
    39841 ggatgcgtcg tgaacgcctt atccggccta caaacgcacg aagattcaac atgttgcaat
    39901 agattttgta ggcctgatga gcgtagcgca tcaggcaatt ctactttact tcgctttggc
    39961 ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg tatccattac
    40021 cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt
    40081 cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt
    40141 accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg
    40201 ctgcggcgtg ctggtagaag caggcaagtt caccttcaca tctaccgtcg ctaacggtgc
    40261 cgccaccata aagataatca gcagaaccaa catcacgtcg ataaacggcg tcacgttgat
    40321 atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta
    40381 tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc
    40441 taggtcacgg ctttgcagca acaatacctg cgctgcaaca tcacccagca tcgctttaaa
    40501 gccgccaatc tggcgtgcaa atacgttata gataacgacc gcaggaatcg ctgccacgag
    40561 gccgattgcc gttgctaaca gagcttctgc gatacccggc gcaacgactg ccaggttagt
    40621 ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag
    40681 accaacgaac ggagaaatcg cgccgatggt tgccagatag ccgttaccgc ggcccatttg
    40741 acgacccact gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc
    40801 gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag
    40861 gcttaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta aggaacgtgc
    40921 ttcagccagc agttgctgct cgcgcttaag gcgacgcttc tgattgaaga actctacgct
    40981 cttactgaag aagattgccc aggtgactac agaggccaaa ataagcccaa tcatcacgca
    41041 cttaacgaca atatcggcgt actgatacat accccagacg gaaaggtccg tctgcattaa
    41101 attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca
    41161 aaacgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt
    41221 tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct
    41281 gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga
    41341 cgtataaaaa caggaatccc gacatggcgg acaaaaagct tgatactcaa ctggtgaatg
    41401 caggacgcag caaaaaatac actcttggcg cggtaaatag cgtgattcag cgcgcttctt
    41461 cgctggtatt tgagagtatg gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg
    41521 agttgttcta tggacggcgc ggaacgttaa cccatttttc cttacaacaa gcgatgtgtg
    41581 aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt
    41641 cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac
    41701 cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg
    41761 atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa atcgtgtttc
    41821 tggaatcgcc gggttccatc actatggaag tccacgacgt tccggcgatt gttgccgccg
    41881 tgcgcagtgt ggtgccggat gccatcatta tgatcgacaa cacctgggca gccggtgtgc
    41941 tgtttaaggc gctggatttt ggcatcgatg tttctattca ggccgccacc aaatatctgg
    42001 ttgggcattc agatgcgatg attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc
    42061 tacgggaaaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca
    42121 gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag
    42181 tggccgaatg gctggcagaa catccgcaag ttgcgcgagt taaccaccct gccctgcctg
    42241 gcagtaaagg acacgaattc tggaaacgag actttacagg cagcagcggg ctattttcct
    42301 ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa ctatctggat aacttcagtt
    42361 tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggca aatcaaccag
    42421 aacatatcgc cgccattcgc ccacaaggcg agatcgattt tagcgggacc ttgattcgcc
    42481 tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc ggttttgcac
    42541 ggattgtata acattgccac ttttggacaa ttttgcagac attttattgt gaaaagtctt
    42601 aaattgttgc gtccgggatc aaggcgtccc ggacgattca ggagtacaat aggcagataa
    42661 aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg
    42721 ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt tgtttacttt
    42781 gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc
    42841 gacagcttgt tgatactggc aggcgcattg attgcccaag gggttatgga ttttctgcct
    42901 acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta tattcagggg
    42961 agctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat
    43021 caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt
    43081 tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg tctgccaaac
    43141 cgccgcttcc agtttttcaa ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt
    43201 tttggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg
    43261 ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac gctgtttgtg
    43321 gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggc tgctgtaccg
    43381 ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac
    43441 accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat
    43501 cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct tatgcagacg
    43561 gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat tatggtggaa
    43621 cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt tttcggtgta
    43681 tttattctca attcgtttca gcacgcggct aatcagactg aagtgagtct ggcgactgac
    43741 cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat
    43801 gatctgtttg ccgagaatac gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc
    43861 cgccgcgcat aaaatctctt ctgataacgt ggcggagtta atcccgctgg ctgccatcga
    43921 cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact gcaaaatatc
    43981 gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa
    44041 cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca cgcgttcatt
    44101 gatataaccg attttatgcc cggaaaagag aattatgatg ccaggctcgt acatcaccgg
    44161 tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca gttctgacaa
    44221 actattttct ttatttttca gtttattaac tttatccgcc agcaagcgac aaatctcttc
    44281 acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg cgcaattttg
    44341 tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg agcattgaga
    44401 gccagggcgc tggcgatcac aatgaaaaac atcaggcaga tcgttctctg ccctcatatt
    44461 ggcccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa cccgcattct
    44521 gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt
    44581 gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc
    44641 cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg cttatgaaac
    44701 gctgatgaac gccgtgaaac tggttcgcga acagaaagtg actttcctgc tggcggttgg
    44761 cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact atccggaaaa
    44821 tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg ccatcccgat
    44881 gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc
    44941 ccgtaaaacc acaggcgaca agcaggcgtt ccattctgca catgttcagc cggtatttgc
    45001 cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta acggcgtagt
    45061 ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg ccaaaattca
    45121 ggaccgtttc gcagaaggca ttttgctgac gctaatcgaa gatggtccga aagccctgaa
    45181 agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa
    45241 cggtttgatt ggcactggcg taccgcagga ctgggcaacg catatgctgg gccacgaact
    45301 gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc ctgcactgtg
    45361 gaatgaaaaa cgcgagacca agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa
    45421 catcactgaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa cccgcaattt
    45481 ctttgagcaa ttaggcgtgc cgacccacct ctccgactac ggtctggacg gcagctccat
    45541 cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg aaaatcatga
    45601 cattacgttg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt tttacgcctc
    45661 aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca ccggagcctg
    45721 ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat taagctacag
    45781 gatggcaatg tcatgcccca gctgggactg ggcgtctggc aagcaagtaa tgaggaagta
    45841 atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac cgccgcggcc
    45901 tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa
    45961 ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga agccctgctc
    46021 gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca ctggcccgtt
    46081 cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca aaaagaggga
    46141 ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg cctgattgat
    46201 gaaactggcg tgacgcctgt gataaaccag atcgaacttc atccgctgat gcaacaacgc
    46261 cagctacacg cttggaacgc gacacacaaa atccagaccg aatcctggag cccattagcg
    46321 caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga taaatacggc
    46381 aaaaccccgg cgcagattgt tatccgctgg catctggata gcggcctggt ggtgatcccg
    46441 aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt ccgtctcgac
    46501 aaagacgaac tcggcaaaat tgcaaaactc gatcagggca agcgtctcgg tcccgatcct
    46561 gaccagttcg gcggctaacg tgcaaattct cccggtggcg gtaatgttcc gctaccggac
    46621 ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga
    46681 atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa caactcatct
    46741 atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac ttctgttttt
    46801 agcagccctg gcaagtttta ccgtacacgc gcagcctcct tcacagaccg tagaacaaac
    46861 agtccggcag atttatcaga actataaatc agatgccagt acgccttatt tcggtgaaac
    46921 cggagagcgg gcgataactt cagcgcgtat tcaacaggcg cttaccctga acgacaatct
    46981 tacactaccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt gtcaggattt
    47041 tggcgatctg gtgttagaaa gcgtagcgat aacccaaact gacgtcgatc atgccgatgc
    47101 cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacacaga cactgaaaat
    47161 ggtggcggaa aatggtcgtt gggtcattga tgatattgtc agcaatcatg gcagcgtctt
    47221 acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt tgcagaaaga
    47281 acagccagaa tcctttgttg ctgaactctt tgaacatatt gctgattata gctggccgtg
    47341 gacgtgggtg gtttccgact cttaccgcca ggcggttaat gccttctata aaaccacctt
    47401 caagacggcc aataatcccg atgaagatat gcaaatagaa cggcaattta tttacgacaa
    47461 tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga
    47521 gaaaaccgcc gattccgccc gcattcatgt tcgttttacg ctgaccaatg gcaacaatga
    47581 agagcaagag ctggttttac agcggcgcga aggtaagtgg gaaatcgctg attttatccg
    47641 cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca gattaaagca
    47701 atgagctgaa ttaaataaca attagccgga acaataaata aaagggaaca ctatatgaaa
    47761 acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat
    47821 gagcaaaagg tcaatcaact ggcgagcaat gtacaaacat taaatgccaa aatcgcccgg
    47881 cttgagcagg atatgaaagc actacgccca caaatctatg ctgccaaatc cgaagctaac
    47941 agagccaata cgcgtcttga tgcacaggac tattttgatt gcctgcgctg cttgcgtatg
    48001 tacgcagaat gataaaaaaa tccccggcag catgtcagtt gccggggatt ttttttaacg
    48061 tccaaccgcc gctttagggc gtttcttcgc accagcattc accggacgag attgcgtaga
    48121 cgacgctttt tttgccgtag caggcgtctg acgctgagtc gccatcggcg tatgtttagt
    48181 caacgccgga cgggtattgc ggttctggcg acgagcttca cgcatctctt caatggttgg
    48241 cgcaggcact aagcaatcgc gacggctgcc aatcagatgc tttttgccca tcgcttccag
    48301 cgcctggcgg attaacggcc agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa
    48361 acgacgctgt ttgtcgccct tcggtacgaa gacgccttcg ctcttataac caatcttcgc
    48421 cagcggtttt ttcccggtgt aatacatggt ggttgagttc gccagcggcg atgggtagaa
    48481 gttctgtacc tggtcgagac ggaaacgatg ctttttcagc cacagcgcca aattcaccat
    48541 atcttcatca cgcgtaccgg ggtgcgcaga gatgaaatac gggatcagat actgctcttt
    48601 acctgcctgt ttcgagtagg tatcgaacag ctctttaaag cggtcatagc tgcccatgcc
    48661 cggcttcatc atcttcgaca acggcccttc ttcggtatgt tccggagcaa tcttcagata
    48721 accgccgacg tgatgggtcg ccaactcttt gatatagcgc ggatcttcca cggcgatgtc
    48781 ataacgtacg cctgaggcga taaggatttt tttaatgcct ttcagatcac gcgcacggcg
    48841 atagaggttg atcgtcggtt cgtggttagt gtccatgtgc ggacaaatat ccggataaac
    48901 gcacgacaaa cggcgacaag tttgttcagc gcgtggcgat ttgcagcgca acatatacat
    48961 gttggcagtt ggcccaccga gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg
    49021 gatcgcttcg atctcattaa tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc
    49081 gtgctcggta atagaacaga aagaacagcc gccaaagcag ccgcgcataa tgttgaccga
    49141 aaaacggatc atttcgtaag ccggaatacg ggcattgcca taggccggat gtggcacgcg
    49201 cttgtacggc agcgcaaaaa cgctgtccat ctcttcggta gaaagcggga tagcaggcgg
    49261 gttgatccac acatagcggt caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt
    49321 ggtttcgtgg tgcagaatgc gcgaagcatg ggcgtacagc actttatcgc ccttcacttt
    49381 ctcgaaagaa ggcagcaata cgtaggtttt ttcccacggt ttctggcgcg gtggctgcac
    49441 gattacagct ttcgcttcct gctttttcgg cgcaacaggt ttgttatccg cacacggcaa
    49501 atcttcgcca tacggatgcg ggattgggtc gattttccct agggtatcaa gacgggtgga
    49561 atccacaccg ctccagccag gcagcgcctc ttttacgata attgcggtat tacgcacatc
    49621 gcggatttca ctaatcggct cgcccatcgc cagacgatgc gctacttcca ccagcggacg
    49681 ttcaccgtta ccaaacatca gcatgtcggc tttcgaatct accagaacgg aacggcgcac
    49741 ggtatcggac cagtaatcat aatgcgcggt acggcgcagg ctagcctcaa taccgccgag
    49801 gatcaccggc acatctttcc acgcctcttt acaacgctgg gtataaacca gcgtggcgcg
    49861 atccggtcgc ttacccgcga cattatctgg cgtgtaggca tcatcatgac gtaaacggcg
    49921 atcggcggta tagcggttga tcatcgaatc catgttgcca gcagtaacac caaaaaacag
    49981 attcggttta cccagacgca taaagtcgtc tttgctgctc cagtccggct gggcgatgat
    50041 cccgacgcga aagccctgcg cttccagcat acgaccgcaa atcgccatcc cgaagcttgg
    50101 gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc agccaagttg
    50161 atccatctct tcacgagaca tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta
    50221 ctgcggccag gagaacaggt cgcgatccgg ttggatcagg gagatagagc tcattttgct
    50281 tccagaaatg ataaaaaaat aatcaaaggc cggggattat aagccggaac gaaagagaaa
    50341 tcgaaaggta ttccatactc gccctcctcg ggcgagtatg aagattacgg taccggattg
    50401 accaacagtt gcccaatcga gccacggtcc gccatttcca gcgtctgact gttgaagtag
    50461 aacgggaagt gcgcccagga aggctgaccg aaataaacaa gcagctccac ttgtccgtca
    50521 acccaaacgg tatctttcca gcctctgtct tccgggaacg gcatcgcgcc attcacgtta
    50581 cggatctgga acattacgcc ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc
    50641 cagcgttccc acgttccttg ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt
    50701 ccattaatac ccgggtcatc acccagactg atatcgcgac tgcgaattgg cgaaccagcc
    50761 atgatttcag ttggcagcaa gcgcatcgga agactgtctg tgaccagcgg cagaaggccg
    50821 gttgggcgca gcgttagcac caaggtagaa accagaatac tggatggctc aaagaagccg
    50881 cgaatacgat caacaatgct cgccgcttca ccacaggtga tcgacacttc atcgccgttg
    50941 ctcatatcca ccagaatctc gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat
    51001 acaggagcag gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg accatcgctc
    51061 atctgtagtt gatagcgacg agagttcgac gcgttcagca gtcgcaagcg cacccagcca
    51121 cgcgagactt caacgtacgg gctttgtaca ccgttaacca gcagcgtatc accaacaaag
    51181 ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt tatccagccg tttatcctgg
    51241 ataatgaccg gaaaatcatc cacaccataa tggttgggga taggcagcga cttgctgact
    51301 tcatcttcca ccagccacat tccggcaagg ccgttataga cctgctgagc cgtgcggttg
    51361 ggagtattgg cgtgatacca cagagtagct gcgttctggc gaataggcag tacgggtgcc
    51421 cagtcagcgt ttggcgacat catccgtgcc ggaccgccca tcagcgggcc tggtacctgt
    51481 agcccggcca ccgtcattga gacattttct gtcaggcggt tgctgtaaat aagcttaaca
    51541 tcgtcgccct tccagacgcg gatagtcggc cccaggtaac gaccattgat tccccagacc
    51601 gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt
    51661 tgcccacggc gagattcaag tagcggcgga acgggtagcg gttgctgttg cccggctgcg
    51721 ctggccttca ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga
    51781 cgccgactga gtgacatatt tgctccatgt aaaactggct aaagtaatac cgtgcaggaa
    51841 agtgttcccc gcaaaaatac agattgccga cttaaacttt tccggcggct tcgcgttctg
    51901 cgacttcttt atcgagctcg gcgatttttt gttccattat cgaacgacaa tgggcagcca
    51961 gctcacgaac ctgatctttg ccatactgac tgacgtcaat tggcggcagc atttcgacaa
    52021 tcaccagacc gttgtgcagt cgattaagat taatcttatt cgaagttgta gagacgcaca
    52081 cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta
    52141 gcaggccgcg accacggctg cgggttcctt ccgggaacat ccagatggaa atacggcgtt
    52201 ttttgaaatg attcacgact tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt
    52261 cgatcaataa gttgccggtt aaccagtaca actgcccgaa gaaggggatc cacagcaagc
    52321 tctttttacc taccgtcacc gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat
    52381 agttgttctg gtggttagcg atatagatag cattgccgta gctttcagca tctgcaggtt
    52441 tgcggcactc aactttcagg ccgaacagtg gcgcaagacg gccaaacata tgcccaaagg
    52501 tggccacatg tttcgggtta cgcgggctga aaaggcagta aatggagccg aatacacaga
    52561 ctaagatgct gtaaatcacg gtaataataa gacgaaagat atatagcata acaccctctg
    52621 aaggtctgag agactggcat tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc
    52681 cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga
    52741 atttttatcg tttccggggg ttgaatacgc tgccggatga cgacttaacg tttcatccgg
    52801 cgttccttgc tagcgggagg aaacagcgcc ctccccggca tattactctt cgctatcacc
    52861 gctgctggca cggcgaggag agtcgatctc aacacgatcg atacgctgca aaccgcgcat
    52921 caacgtaccg cggcgtccac gttcgccagt gactttctgt agctcttccg gacgcagttt
    52981 aattttgcgt ttcccaacat gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg
    53041 cgccagacca tcctcgccac gcgcggcttc tgccgatgga atgttgataa tcttgttgcc
    53101 tttgcccttc gacagctgcg gcagatcgct taccgggaac atcaacatac ggcctgcctg
    53161 agtgattgcc agcagcatat cggaagcatc ttcaatcacc accggcggca taacatgggc
    53221 attttccggt aaggtgatca aagccttacc cgcacggtta cgcgctacca gatcgttaaa
    53281 ggtgcagacg aaaccgtaac ccgcatcgga agccatcagc agtttctgat cgtcgctttc
    53341 catcagcata tggtcaacgg tcgccccagg cggcaacgtt aatttgccgg tgagtggttc
    53401 gccctgacca cgcgccgacg gcagcgtaat cgggtcgatg gcatagctac gaccggtgga
    53461 atcaacaaac actaccggtt ggttactctt acctttcacc gccgctttga agctatcacc
    53521 cgctttataa ttcaggcccg gcgcgtcgat atcatggcct ttagcgctgc gtacccagcc
    53581 catctgcgac agcacaatgg tgacaggttc agacggcagc atgtcgtgct cgctcatcgc
    53641 tttcgcttct tcgcgttcct gcaacggcga acgacgatcg tcaccgtagg cttgcgcgtc
    53701 tgcctgcagt tctttcttca gcaggttatt cattttacgc tcggaagcca aaatgccctg
    53761 caactggtcg cgctcttttt ccagctcact ctgctcaccg cgaatcttca tctcttccag
    53821 tttggcaaga tgacgcagtt tcagttcgag gatcgcttcc gcctgggttt ccgtaaggcc
    53881 aaaccgcgac atcagcgccg gtttcggttc atcttcgtta cgaatgatct caatcacttc
    53941 gtcgatgttg agaaacgcca ccagcaaacc ttccaggata tgcaggcgct tgaggacttt
    54001 ctccagacga tagttcagtc ggcggcgcac ggtatcgcga cggaacacca gccattcgga
    54061 gaggatttcc agcaggtttt tcaccgccgg acgaccatcc agaccgatca tattgaggtt
    54121 aatgcgatag ctcttttcca gatcggtggt agcgaagagg tggttcatca cctgatccat
    54181 atccacgcgg ttggaacgcg gcacaatcac cagacgggtc gggttctcgt ggtcagattc
    54241 atcgcgcaga tcgtcaacca tcggcagctt tttgttgcgc atttgcgcag caatttgctc
    54301 cagtacgcgc gcacctgaaa cctgatgcgg taatgcgctg ataaccaccg cgccatcttc
    54361 tttcttccac accgcgcgca tacgcactga accacgtccg ttctcgtaga ttttacgaat
    54421 ctcggcgcgc gaagtgataa tttccgcttc agtcggataa tccggcccct gcacgatatc
    54481 cagcagctga tcgagcgtag ttttcggctg gtcgattaat gcgattgccg cctgagctac
    54541 ttcacgcagg ttatgcggtg gaatatcggt cgccatgccg acggcaatac cggtggtgcc
    54601 gttaagcaaa atgtttggca gacgggcagg tagcattttc ggctcctgca aagtgccgtc
    54661 gaagtttggc acccagtcag ccgtcccctg ccccagctcg ctcaatagca gctcggaata
    54721 tttcgacaac cgggattcgg tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc
    54781 gccccagttc ccctgaccat caaccagcgg ataacggtaa gagaacggct gcgccatcag
    54841 gaccatcgct tcataacagg cgatatcgcc gtgcggatgg tatttaccca gtacgtcacc
    54901 gacggtacgg gccgattttt taaatttggc gctggcattc aggcccagtt cagacatcgc
    54961 atacacaatg cggcgctgaa caggtttcag accatcacca ataaacggca acgcacggtc
    55021 catgatcacg tacatggagt agtttaagta ggcgttttcc gtaaattcat gtagcgcaag
    55081 gcgctctgcc atatcgctca ttaattctga ttcctcaact tattcgccag ccttcaaagg
    55141 ggcaatattg ccggagatac tacattatct gccgcgtcga gtcacaggag atcagcgtga
    55201 atagcgccgc cggacgtgct ccgacggcga taaaggcatc aatgtgcctt gatatacaac
    55261 tctttgctgt agacttgatc ggcatagagg gcagcacaag gttgcgccca tgccacacca
    55321 ctcggcatgg gggtccgctc cgggcgattc gagaagttga ggttatgcga gtccagggaa
    55381 gcctaaagca tcaaagtagt cgactgtaag tatacggttc agcccagttt agctgtgtct
    55441 ccaccagtgc cccgcgcctc ctgccataag tgcggcttgc tcatggatat ccccgagacg
    55501 gcttacggcg caccggataa taaatttcgc gtatacatgg tattccttac cggagaaatt
    55561 ataagcccaa caacaatacg cctgtgacat tgttcgctca aacttatcgc gagtgatttg
    55621 gctcacactc cggtaaagat tccccatcac gccaacggca cataaacatc cgtttccagt
    55681 cgctgctctg tcaccccttc ggcaagattg gtgtagtgaa acagaatcgg tgctttacgc
    55741 attttctcgc cgcttgcagg cagccagtgg cgaataatgc cccatatcgt gtgtgaaata
    55801 tcgtccagct cgccaacgtg gcgggccacg gcataacgtc caccggtaag ttcaccattg
    55861 ctcacaccat aacgattatc gggaatcggt tcgctaacgc tgccgcagat atcaaagcga
    55921 aacgcttccg gtgcggtggt ggcaggggcg tcccaggcga cgccaaaagt ctggctttgg
    55981 ttaacaggag aaagccccgt ctctttacgc cacatgataa atttcgccac actgtagttg
    56041 agcaactcag ggctacagcg gtgcggcaac atcgccacag ggattgatgg gaaatcgata
    56101 atgtttacat ccagtgtcag gtttgtcatt ttgcctccct gcgaaattat tcccgggaac
    56161 gcgtcccggg agcggtaaca attacggatt tactttgcgg atctgtttga cgtcaatttc
    56221 aacagaattc cagtctttat cgacttcacc ctgaatctca accgtatctt tcggcgtcac
    56281 cgtcacaccg ttccagcgtt tgtgatcgat atcaacattg atagtaccgc tggcatcttt
    56341 aaacacgtag agatcgtcag agatgcgttc aacgatattg ccgcgcaggg ttacccaggt
    56401 gtcgtcacgc agggattttg cgctttctac agtcgttacg ctgccgttcg gcccctgaaa
    56461 tcctccggcc tgactttgcg ttaccgatgg gccagaaaaa ccgccctgct ctgctgccat
    56521 caccggtgca ctgcacaggg ccattactgc gattactgct gcgaattttt tcatgtttat
    56581 tactcccttt aatgtctgtt tccgagcatt taacaagata gtccttaaca acttcttaag
    56641 ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca acacggttta ctggcagcaa
    56701 atacggttat cgcagggatg aaaaaatgcg aattttactg atagaagatg acatgctgat
    56761 tggtgacggc atcaaaactg gccttagtaa aatgggtttt agtgtcgact ggtttacaca
    56821 aggtcgtcag ggaaaagagg cgctttatag cgcaccttat gatgcggtga tcctggattt
    56881 aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa tggcgagaga aaggtcagcg
    56941 tgagccggta ctgaacctga ccgcgcgtga tgcgttagcg gaacgtgtag aagggctgcg
    57001 tctgggagct gacgattatc tgtgtaaacc ttttgcgttg atagaagtcg ccgccaggct
    57061 ggaggctctg atgcgccgaa ccaacggcca ggccagcaac gagctgcgcc acggtaacgt
    57121 catgctcgac cccggcaaac gtatcgccac gctggctggc gaacccttaa cactgaaacc
    57181 aaaagaattt gccctgctgg aattactgat gcgtaacgct ggtcgggtac tgccgcgcaa
    57241 actgattgaa gagaaactgt atacctggga cgaagaggtc accagtaatg ccgttgaagt
    57301 gcatgtgcat catctgcgac gcaaactcgg tagtgatttt attcgtaccg tgcatggtat
    57361 tggctacaca ttaggtgaga aatgaaattt acccaacgtc ttagtctgcg cgtcaggctg
    57421 acgctaatct ttttaattct ggcctcggtg acctggctgc tttccagctt tgtcgcctgg
    57481 aaacaaacaa cggataacgt cgatgaattg ttcgacaccc aactgatgct gtttgccaaa
    57541 cggttaagta cgctcgatct caacgaaatc aacgcggcgg atcgcatggc acagacgcca
    57601 aataaattaa aacacggtca tgttgatgac gatgcgctga cctttgccat ctttacccac
    57661 gacggcagaa tggtccttaa tgatggcgat aacggagaag atattcccta tagctatcaa
    57721 cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg atccttggcg ttttgtctgg
    57781 atgacctcac ctgatggcaa atatcgcatc gttgttggcc aggaatggga ataccgtgaa
    57841 gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt ggctggtcgc actgccgatt
    57901 atgttaatca tcatgatggt actactgggt cgtgaactcg cgccgctgaa caaactggcg
    57961 ctggcactac gtatgcgtga ccctgactcg gaaaaaccac taaacgcgac tggcgtaccc
    58021 agcgaagtgc ggccactggt tgagtcgcta aatcaactgt tcgcccgcac acatgcgatg
    58081 atggttcgag aacgacgctt tacctccgat gcagctcacg aactacgtag cccgttaaca
    58141 gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg atgatccgca ggcgcggaaa
    58201 aaagcactgc tccaattaca ttccgggatc gatcgcgcta ctcgtctggt tgatcaactg
    58261 ctcacgctat cgcggctgga ctcactggat aaccttcagg acgtcgcgga gatcccgctt
    58321 gaagatctcc tacaatcgtc ggtgatggat atttaccaca cggcgcagca ggcgaaaatt
    58381 gacgtgcgac tgacactcaa tgcccacggc atcaaacgca ccggacaacc gctattgcta
    58441 agtttgttgg tgcgaaattt gctggataac ggccccggtg tgacaccaga ggcactggcg
    58501 cgaattggcg aacgcttcta tcgcccaccc ggacaaaccg ctaccggcag tggacttggg
    58561 ctatcgattg tccagcgaat cgccaagctg catgacatga atgttgagtt tgggaatgcg
    58621 gaacaaggtg gatttgaggc gaaggtaagc tggtaacttc tctgtctatg agagccgtta
    58681 aaacgactct catagatttt actaatagca aaatataaac cgtccccaaa aaagccacca
    58741 accacaaacc agacaaataa cgagcgagaa tacatatagc caaaactcga acatttcccg
    58801 cataaagagt tttcttaaga taagaataat aagtggcgta agaagaaaaa atgctgcaat
    58861 cattgcttgc aatatacgat cttcgcctga aacggaaggt atactgcaaa tattgcctcc
    58921 cgactcaagc atccagtcat acttattgaa agcaaaaaaa gaatctatta accaataagt
    58981 aagcattata tatggcaatg cttcaaatat tgttttaatt ttctgcttta acatatatca
    59041 gtacgctcat aaaaatccat ttataattat caacatgaca cgttgatatt ttcatctttt
    59101 acttattcaa agccatattt tcaatcccca aaaaaaccga accaaacgtg taaattattc
    59161 cggtgcgagt aagaattatg aggaatggct atcagtattg tcattttcag aaaatattta
    59221 tcctgcatcg gtgagtcaga gctagatcag acttttgcta aattcgcaaa agactttgca
    59281 cattttgcta atttcacagt accgctctgt gacgtactat agtcgacaaa cgtctcacct
    59341 tgagattaaa aaatgagcaa catcctgatt atcaacggcg cgaaaaaatt cgcccactcc
    59401 aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg gcacactgcg cgacctcggg
    59461 catgatgtcc gcatcgttcg cgccgacagc gactacaatg tcaaagcgga agtacaaaac
    59521 tttctctggg ctgatgtggt gatctggcag atgccaggct ggtggatggg cgcgccgtgg
    59581 acagtgaaaa aatatattga tgatgtattc accgaaggtc acgggactct gtatgccagc
    59641 gatggtcgta cccgcaaaga tccgtcgaaa aaatacggtt ccggcagcct ggtacaggac
    59701 aaaaaatata tgctttctct aacctggaac gcaccaatgg aagccttcac cgaaaaagat
    59761 cagttcttcc acggcgttgg cgttgacggt gtgtatctgc cgttccataa agcaaaccaa
    59821 ttcctcggta tggaaccgct gccgacattt atcgctaatg acgtgataaa aatgcctgat
    59881 gttccccgct atactgaaga atatcgcaag catcttgtgg aaatttttgg ttaactagag
    59941 ctcaggcttt agaaggagtt aaccatgctt accgtaatcg cagaaatccg tactcgtcct
    60001 ggtcaacatc accgtcaggc ggtattggat cagtttgcta aaatcgttcc aaccgtactg
    60061 aaagaagaag gttgccacgg ctatgcgcca atggtggatt gcgctgctgg cgtgagtttc
    60121 cagtctatgg caccggattc tatcgtgatg attgagcagt gggaaagcat cgcgcacctt
    60181 gaagcgcatc tgcaaacccc gcacatgaag gcgtacagcg aagccgtaaa aggcgacgtg
    60241 ctggagatga atatccgtat tctgcagcca gggatttaat cctgccttgt ttgcccggcc
    60301 atcctgaccg ggcaatgttc tttcctttaa acctcaatct ccgccatgtc gcctttctct
    60361 tgcaaccagt tgcggcgatc ttccgagcgt ttcttcgcca gcagcatatc catcatcgcg
    60421 tcagtacgct gatcgtcttc atcatcgata gtcaactgca ccagacggcg agtgttcgga
    60481 tcaagcgtgg tttcacgcaa ttgcatcggg ttcatttccc ccagaccttt aaaacgctgg
    60541 acgttcggct tgcctttctt gcgttttaat tgctcaagta cgccctcttt ctcttcttcc
    60601 gtcagcgcgt aataaacctc tttcccgaga tcaatacggt agagcggtgg cagtgcgacg
    60661 taaacgtgac cgtgtttcac caacgcgcgg aagtgtttta cgaacaaagc gcagagcagc
    60721 gtggcaatgt gcagaccatc agagtccgca tccgcgagga tacagatttt gccataacga
    60781 agctggctca gatcgtcgct gtcaggatcg ataccgatcg ctaccgaaat atcatgcact
    60841 tcctgcgagg ccagcacttc gtcggaagaa acttcccagg tgttaaggat cttacctttc
    60901 agcggcatga tcgcctgata ttcacgatcg cgcgcctgct tggcagatcc gcctgcggag
    60961 tcaccttcca cgaggaacag ttcggtacgg ttaaggccct gcgcggtaca atcagccagt
    61021 ttgccaggca gcgccgggcc gctggtcagc tttttacgca ccactttttt agccgcacgc
    61081 atacgacgct gggcgctgga aatcgccatc tctgccagca actccgccgc ctgaacgttc
    61141 tggttcagcc acaggatgaa ggcatctttc accacgcccg aaacgaatgc cgcacactga
    61201 cgcgaagaga gacgctcttt ggtctgcccg gcaaactgcg gatcctgcat tttaaccgac
    61261 agaacatagg cgcagcgatc ccagatatct tccgccgaca gcttcacgcc gcgcggcaga
    61321 atgttgcggt attcacagaa ctcacgcatc gcgtccaaca ggccctgacg cagaccatta
    61381 acatgggtac cgccctgcat cgttgggata aggttgacgt agctttcggt cagcagttca
    61441 ccgccttccg gcagccacag tagcgcccag tccacagctt cagtatcgcc agcgaaatta
    61501 ccgataaacg gtttttctgg cagcgtcggt aaaccgttta ccgcttccgc caggtaatca
    61561 ttcagaccgt cctgatagca ccagcgctgt tcggtgttgt tgatctcatc tttaaaggta
    61621 atctcaacgc cggggcacag tactgctttg gccttcagca catgcgtcag gcgtgaaaca
    61681 gaaaaacgcg ggctgtcaaa gaaggtttca tccggccaga agtgcacgct ggtaccggta
    61741 ttgcgtttac cgcaagtgcc gacaacctgt aaatcctgca ccttttcgcc attttcaaag
    61801 gcgatgttat aaacctgacc atcgcggcgc acgttaactt ctacgcgctt cgacaaggcg
    61861 ttaactaccg aaatccccac gccatgcagg ccgccagaga actggtaatt tttgttagag
    61921 aatttaccgc ctgcatgcag acggcaaaga atcagttcaa ccgccggtac tccttcttcc
    61981 gggtgaatat ccaccggcat cccgcgccca tcgtcaatca cttctaacga ctggtcagca
    62041 tgtaagatta catccacgcg ttttgcgtga cccgccagtg cttcatcgac actgttatca
    62101 ataacttctt gccccaaatg gttagggcga gtggtatcgg tatacatccc cggacggcgg
    62161 cgaaccggct caagcccggt gagtacctca atggcatcag cgttataagt ttgcgtcatg
    62221 gtttaagtta gcaattcgag ttgatcgtca gagatggtgc agaccaagaa aatcgacgat
    62281 cgggttgaaa taatcttcga agcccgtgaa tgcgtggttg ccgccttcta tgacagtctg
    62341 gcggcaggaa gcatagtacg ccaccgcctg gcggtaatcc agcacttcat ctcccgtctg
    62401 ttgcagcagc cagatcaaat ccggcgcttc cagcgggtca atctgcatga ctttgagatc
    62461 gtaaatatgg cgtgactcta gcacatattg ctgcccggtg taggggttct cgttctgacc
    62521 gagatagtcc gtcagcaatt caaacgggcg taccgccggg tttaccacca ctgcgggcag
    62581 cataaaacat tgcgacaacc aggtggcgta atatcccccc agtgacgaac cgacaatacc
    62641 cagcgaatcg ccgccatgtt ccaggacaat ggattccagc agttctgccg cgtcggaagg
    62701 atacggcggc aactgcggaa tgatcatctc aacgtcaggg tgatgttccg ccagccagtt
    62761 ttttaacaag ctcgcttttg cagagcgcgg cgagctgttg aaaccgtgta aataaagaag
    62821 cgtagacatc agtagccttc tgaagcggta tcaggttgga aacgtgtgtc cgccaggcga
    62881 tgcacctcgg tggtcagcgt gccatcagca tgtaactcga gagtacgcca gccgggcgcg
    62941 atagtatcca gcgtaaagtt ggaacagtgc ggcttaaact gcacacaggt cgacggcgtt
    63001 gccagcaggc ggcgaccgtt ccagtcgaga tccagctcct gatgaatatg accgcacagc
    63061 aagtatttga cgtgcggaaa cttcgccagc acggtatcca gttcgcccgc gttacgcaga
    63121 ctgtgttgat cgagccaact acaacccgca ggtagcggat gatgatgcag cagcagcaac
    63181 gtatggcgtt ctggcgcatc ggccagttta cgttccagcc actcaagctg aaactcgctc
    63241 agctcaccgt gcggcacgcc aaacacctgg ctatccagca acaggatttg ccattgctca
    63301 ccaataaaca cccgcttcgc cggggagata cccgcatcct gtaacgcgct gtacatcgcg
    63361 ggctggaaat cgtggttgcc cggcagccag acgcagggcg cacgaaaact tgcgatgcct
    63421 tcagcgaaat gctgataggc cgcagaggat tgatcctgcg ctaaatcacc tgtcgcgaca
    63481 atcaggtcga attcgtgctg gtgtggacga atcgcctcca gcaccgcctg gtagctttcc
    63541 caggtgttta cccctaacag ggcttcgtgc ttttgtgcaa acaggtgagt atcggtaatt
    63601 tgtaaaatcc ttcctctggc ctcaccagcc agaggaaggg ttaacaggct ttccaaatgg
    63661 tgtccttagg tttcacgacg ctagtaaacc ggaatcgcca tcgctccatg tgctaaacag
    63721 tatcgcaacc agtccgctaa aaactggtta atttgatgct tttcgtcgcg ttgatgcaac
    63781 tttttattag gataatcata ccgcgctttg aagcgaaaaa tctgctggct tgaacacact
    63841 tcagccacca tcgcgtcatg atacagacgc accgtcattg acggaaggct ccagtaactg
    63901 atcgcgggcg cagtctgttc tattgtcacc agggtagtgt atcgggtcga ttccacaatc
    63961 gtcagccgat attgtgcgtt tgccacctga tagcttacag tttcgccggg tgcgtcattg
    64021 cgcggtaaca aacggcgcaa ttgtgaaaag ttcatctcgc acaggcgcat catttcagga
    64081 aagtcaggtg tgtaacgctt catttatgcc cactcatttt ttaacgcttg atgatgcagc
    64141 tgcagccatt gcaaagcgat gaccgacgct gcgttgtcga ttttcccctc ttctacccac
    64201 tggtatgcct gttcccggct taccacatga acgcgaatat cttcgttttc atcagccaga
    64261 ccatgaatac cgcttgcggt cgtggcgtcc acttcgccca ccataattga cgaacgctca
    64321 ctggtgcccc ccgggcttgc caggaaactt aacaccggtt tggtccgttt gactatcagt
    64381 cccgcctctt caatcgcttc gcgacgggca acatcttcca cactttcacc ctcttcaatc
    64441 atcccggcaa ccatctccag tagccagggg gtttcgctgg tgtcgtacgc ggcaatccga
    64501 atctgctcaa tcagcacaac ttcatcacgc actgggtcaa agggtagcaa gactgcggcg
    64561 tgaccgcgct caaaaatttc ccgccgtacc tcatgactca tttgcccgtt gaatagacga
    64621 tgacgaaatc tataaagatc taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata
    64681 atttctacat cgtttttgcc aaatgtaacg ggcaggttgt ctggcttaag cattgttaat
    64741 gtcctagcac taatagtgaa ttaaatgtga atttcagcga cgtttgactg ccgtttgagc
    64801 agtcatatgt taaattgagg cacattaacg ccctatggca cgtaacgcca accttttgcg
    64861 gtagcggctt ctgctagaat ccgcaataat tttacagttt gatcgcgcta aatactgctt
    64921 caccacaagg aatgcaaatg aagaaattgc tccccattct tatcggcctg agcctttctg
    64981 ggttcagttc gttgagccag gccgagaacc tgatgcaagt ttatcagcaa gcacgcctta
    65041 gtaacccgga attgcgtaag tctgccgccg atcgtgatgc tgcctttgaa aaaattaatg
    65101 aagcgcgcag tccattactg ccacagctag gtttaggtgc agattacacc tatagcaacg
    65161 gctaccgcga cgcgaacggc atcaactcga acgcgaccag tgcgtccctg cagttaactc
    65221 aatccatttt tgatatgtcg aaatggcgtg cgttaacgct gcaggaaaaa gcagcaggga
    65281 ttcaggacgt cacatatcag accgatcagc aaaccttgat cctcaacacc gcgaccgctt
    65341 atttcaacgt gttgaatgct attgacgttc tttcctatac acaggcacaa aaagaagcga
    65401 tctaccgtca attagatcaa accacccaac gttttaacgt gggcctggta gcgatcaccg
    65461 acgtgcagaa cgcccgcgcg cagtacgata ccgtgctggc gaacgaagtg accgcacgta
    65521 ataaccttga taacgcggta gagcagctgc gccagatcac cggtaactac tatccggaac
    65581 tggcggcgct gaatgtcgaa aactttaaaa ccgacaaacc acagccggtt aacgcgctgc
    65641 tgaaagaagc cgaaaaacgc aacctgtcgc tgttacaggc acgcttgagc caggacctgg
    65701 agcgcgagca aattcgccag gcgcaggatg gtcacttacc gactctggat ttaacggctt
    65761 ctaccgggat ttctgacacc tcttatagcg gttcgaaaac ccgtggtgcc gctggtaccc
    65821 agtatgacga tagcaatatg ggccagaaca aagttggcct gagcttctcg ctgccgattt
    65881 atcagggcgg aatggttaac tcgcaggtga aacaggcaca gtacaacttt gttggtgcca
    65941 gtgagcaact ggaaagcgca catcgtagcg tcgtgcaaac cgtacgttcc tccttcaaca
    66001 acattaatgc ttctatcagt agtattaacg cctacaaaca agccgtagtt tccgcgcaaa
    66061 gctcattaga cgcgatggaa gcgggctact cggtcggtac gcgtaccatt gttgatgtgt
    66121 tggatgcgac caccacgctg tacaacgcca agcaagagct ggcgaatgcg cgttataact
    66181 acctgattaa tcagctgaat attaagtcag ccctgggtac gttgaacgag caggatttgc
    66241 tggcactgaa caatgcgctg agcaaaccgg tttccactaa tccggaaaac gttgccccgc
    66301 aaacgccgga acagaatgct attgctgatg gttatgcgcc tgatagcccg gcacccgtcg
    66361 ttcagcaaac atccgcacgc actaccacca gtaacggtca taaccctttc cgtaactgat
    66421 gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa cgtaaagata cgggttatct
    66481 gccgcattct tcccccttct cgcttcaatt tcgaccagcc atcctctatt ctgatgggta
    66541 tttaccactg gtcccggaag acaaaaatga aacggacaaa atccatacgc cacgcatcgt
    66601 tccgcaaaaa ctggagcgca cgccatctga caccagtcgc tctcgcggtt gccactgttc
    66661 ttatgctggc tggctgtgaa aagagtgatg aaacagtgtc tctctatcaa aatgctgacg
    66721 actgttcagc tgcaaaccca ggcaaaagcg ccgaatgtac caccgcgtac aacaatgcgc
    66781 tgaaagaagc cgaacgtact gcgccgaaat acgccacccg tgaagactgt gttgctgaat
    66841 ttggtgaagg tcagtgtcag caagcaccag cccaggctgg catggcacca gaaaaccagg
    66901 cgcaggctca gcaatccagc gggagtttct ggatgccgct gatggccggt tacatgatgg
    66961 ggcgtctgat gggcggcggc gcgggatttg cacagcagcc gctgttctcc tcgaaaaacc
    67021 cggccagtcc ggcttacggt aaatataccg acgcgacggg taaaaactat ggcgcagccc
    67081 agccaggccg caccatgacc gtaccgaaga cggcaatggc accaaaaccg gcgaccacca
    67141 ctaccgttac ccgtggcggt tttggtgaat ctgttgccaa acaaagcact atgcagcgta
    67201 gcgcaaccgg cacctcttct cgttcaatgg gtggctgata ccgatggaaa gagtcagtat
    67261 taccgagcgc ccggactggc gtgagaaagc ccacgaatac ggtttcaatt ttcacaccat
    67321 gtacggcgag ccgtactggt gtgaagatgc ttactacaag ttgaccctcg cccaggttga
    67381 aaagctggaa gaagtcaccg ccgaactgca ccagatgtgc ctgaaagtgg tggaaaaagt
    67441 gatcgccagc gatgagctga tgaccaaatt ccgcatacca aaacacacct ggagttttgt
    67501 gcgtcaatca tggctgactc accagccatc gctttattcg cgtcttgatc tggcgtggga
    67561 tggcactggc gaacctaaac ttctggaaaa taacgccgat acgccaacgt cactatacga
    67621 ggcggcgttc tttcagtgga tctggctgga agatcagctt aacgccggta acttgccgga
    67681 gggcagcgac cagtttaaca gtctgcaaga aaaactgatc gatcgcttcg ttgagctgcg
    67741 tgaacagtat ggcttccagt tgctgcatct cacctgctgt cgcgacacgg tggaagatcg
    67801 cggaaccatt cagtatttgc aggactgcgc aaccgaagct gaaatcgcca ccgagttcct
    67861 ctacatcgac gagataggtt taggcgaaaa aggccagttc acggatttac aggatcaggt
    67921 gatttctaac ctgttcaagc tttatccgtg ggaatttatg ctgcgcgaaa tgttctccac
    67981 caagctggaa gatgctggcg tacgctggct ggaaccggcg tggaagagca ttatctccaa
    68041 caaggcactt ctaccgctac tgtgggagat gttcccgaat cacccgaacc tgctgcccgc
    68101 ttattttgcg gaagatgatc atccgcaaat ggaaaaatat gtggttaaac cgatcttctc
    68161 ccgtgaaggc gcaaacgtgt cgatcattga gaacggcaaa accattgaag cagcggaagg
    68221 tccgtatggc gaagaaggga tgattgttca gcaattccac ccgttaccga aattcggcga
    68281 cagctatatg ctgattggta gctggctggt gaacgatcaa cctgccggaa ttggcattcg
    68341 tgaagaccgt gcgttgatca cccaggatat gtctcggttt tatccacata tttttgttga
    68401 ataagccacg ataccggatg gcactcgcca tccggtaatt gttagcctat ctgcaccgac
    68461 agcatactta aactgcccat ttctatacca tcgaccggaa tggtaattgg ctcctgccca
    68521 tcccacacac ctaacacata caacaacggc agatagtgct ctggcgttgg gttcgataac
    68581 gcgccacctt catggtcgag ataattcacc agaggatgct gctctaccgg cccttgccac
    68641 gtcagattcg ctttcacata ctcattaaac gacgtcgccc acggatacgg tgaactatca
    68701 ccgtgccact tcactgtgcg caggttatgc accacgttac cgctggcgac caacattatt
    68761 ccttcatcac gcagcgccgc gagtttgcgc cccatttcga aatgccaggc ggcaggtttg
    68821 ctactgtcga tactcaactg caccataggg atatcggcgt caggatacat cttaatcagc
    68881 acgccccacg agccgtggtc aaagccccag gcttctttat ccagcgtcac cgggatcggc
    68941 gctaacagct caaccagacg ctgtgccagc gcaggcgaac ccggagcagg ataatgcgta
    69001 tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg
    69061 gtcactcctg ttccacgggt aaaccagtga gccgaaacca ccacaatcgc ttgcgggcgt
    69121 ggcaatgtca tccccaactt ctgccagctg cgggtataca aattatcttc cagcacgttc
    69181 atcggactac cgtgacctaa aaacaatgct ggcatacgtg ttgaagacat gatgatatcc
    69241 ttaactaaag gtgtcatttt gatatcctca caatacgctt gttcggcgga gtaagaaccc
    69301 ggataacaat gatgatgatc atcagttatt ttgacgatct gcctgaaggt gaagatttat
    69361 aaggagttgt cgatgtcagt acctctcatt ctgaccatac tggcgggggc agccacgttt
    69421 attggcgcgt ttctcggcgt tctcggacaa aaaccctcga accgcttact ggcgttttcg
    69481 ctaggttttg cggcggggat catgttgctc atctcattaa tggaaatgct tcctgccgca
    69541 ctagcagctg aaggaatgtc gccggtgttg ggttatggaa tgtttatctt cggtctgctt
    69601 ggctactttg gcctggaccg catgttgccg catgcgcatc cgcaggattt aatgcaaaaa
    69661 tcggtgcagc tgttgccaaa atcgatcaag cgcacagcca ttctgctcac tctcggcatc
    69721 agtctgcata acttcccgga agggattgcc acctttgtca cggcgagcag caacctggag
    69781 ctgggatttg gcatcgcact ggccgtcgcg ttgcacaata tccctgaagg tctggcagtg
    69841 gcaggcccgg tttatgcggc aacggggtct aaacgcaccg cgattctgtg ggcggggatt
    69901 tctggactag cagaaattct tggtggtgtg ctggcgtggt taatcctcgg tagcatgatt
    69961 tccccggtgg tcatggcggc aatcatggcg gcggttgcag gaattatggt ggcgctctcg
    70021 gttgatgaat taatgccgct cgccaaagag attgacccta ataataaccc cagctatggc
    70081 gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac ggcgggaatt
    70141 ggttaaaaac aacatcagaa gttgtccagc acatggacaa cttctgccag cttcacgaaa
    70201 gccttattct accttttcgg ctgtctcttc tctcgtactg tttaagtatt tgttccgcgc
    70261 ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa taacgtttca aatgcaggaa
    70321 ataacccacc acaaccagag caatcaatac aatccagtac caggcgataa acatcctttt
    70381 acccttaata tttaacaata ctaaacattt agcgcataaa tttcacatat cctttttcgg
    70441 atatattcat atggtcgcat aataacggaa caattatatg attttgatca taatgaaaaa
    70501 gaatgcctga tattaatgaa attttctcat agaaagagaa gcggaattaa catcttgcat
    70561 agcagggcaa aataaaaacc gaagccagct acaacttcgg tttcttgatt aaggcagtaa
    70621 ttaagcagcg gttttcagct ggctttacgc tcatgtgcct gacggtatgc caccaggtct
    70681 tcaatagtca cgagcgccat attgtgttta ttggcaaact caatgcactc tggtgcacgc
    70741 gccatcgtgc catcgtcatt agtcagctca cacagcacac cagccggttt aaagcctgcc
    70801 agcgtcatca aatcaatagt cgcttcagta tgaccgccac gcgtcagtac accacctgcc
    70861 tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat cagaaggttt tgcgccatcg
    70921 gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag aaacaccggt agtcacacct
    70981 tcagccgctt caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt attttctacc
    71041 atcattggca gatcaagttg tttacggcga tcttcagtga tgcacaggca aacaataccg
    71101 ctaccgtggc gaatggtcag cgccatctgc tcaacggtca tggtttctgc cgggaagatc
    71161 atatcgcctt cgttttcacg gtcttcatca tcaagcacca ttacaccgcg tccttcacgc
    71221 agcgcagcca gtgcattttc aacacgttcg aaaggcgtac caaaagagga aagtagcgtc
    71281 tgattcatgg taaaaaaaac ctcactaaaa ttatggttac cagaatcagg gcagtcttag
    71341 gagtggcggc atatagccaa aataacgtga gcgggtccat gcccgacaga atcgttactc
    71401 tctcccatcc ggactctaac cgtcggcccc ggaattacac cggatctgct gtcctttgag
    71461 ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg tggggaattt
    71521 cgccccgccc tgagaataag cggattcact ataacgctaa tgattagcgg cagcaacgca
    71581 tagcttcaca taattctggt ttatgactta cccttatcgc actacaatgg tactcaacac
    71641 ctatcagtac agggaaacta caatgattga cccgaaaaaa attgagcaaa tcgctcgcca
    71701 ggttcacgaa tcaatgccta aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat
    71761 ccgccaaacc ctacaagctc agctgacgcg cctcgacctt gtaagccgtg aagagttcga
    71821 cgtccaaacg caagtcctgt tacggactcg tgagaaactg gcgctgcttg aacaaagaat
    71881 cagcgaactg gaaaatcgca gtactgagat caaaaagcag ccagatccag agactctccc
    71941 cccaacgctg taaacatttg tctttaatgc actgtaaatt atgaattaat cctctaattt
    72001 acagtgtttt ctattacatt atcgcttaat cccattcaga gtatcatcac aaaaatgaat
    72061 gattgcaatt gaaaatataa ttgttatgta ttctttaagt tttacttaat cgata
//
LOCUS       DAJTRN010000005        70545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRN010000005 DAJTRN010000000
VERSION     DAJTRN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..795
                     /locus_tag="OO328_000339"
     CDS             <1..795
                     /locus_tag="OO328_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5929034.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OO328_000340"
     CDS             806..1897
                     /locus_tag="OO328_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5929035.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO328_000341"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO328_000341"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5929036.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OO328_000342"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OO328_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5929037.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OO328_000343"
     CDS             4859..5254
                     /locus_tag="OO328_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5929038.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OO328_000344"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OO328_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5929039.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OO328_000345"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OO328_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5929040.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OO328_000346"
     CDS             complement(6563..7603)
                     /locus_tag="OO328_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5929041.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OO328_000347"
     CDS             complement(7676..8311)
                     /locus_tag="OO328_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5929042.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OO328_000348"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OO328_000348"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5929043.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OO328_000349"
     CDS             complement(8937..9380)
                     /locus_tag="OO328_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5929044.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO328_000350"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO328_000350"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5929045.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OO328_000351"
     CDS             complement(9720..10223)
                     /locus_tag="OO328_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5929046.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OO328_000352"
     CDS             complement(10217..10741)
                     /locus_tag="OO328_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5929047.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OO328_000353"
     CDS             10950..11945
                     /locus_tag="OO328_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5929048.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OO328_000354"
     CDS             11954..12832
                     /locus_tag="OO328_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5929049.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OO328_000355"
     CDS             12913..13920
                     /locus_tag="OO328_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5929050.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO328_000356"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO328_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5929051.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO328_000357"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO328_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5929052.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO328_000358"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO328_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5929053.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO328_000359"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO328_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5929054.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO328_000360"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO328_000360"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5929055.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO328_000361"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO328_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5929056.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO328_000362"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO328_000362"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5929057.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO328_000363"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO328_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5929058.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO328_000364"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO328_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5929059.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO328_000365"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO328_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5929060.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO328_000366"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO328_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5929061.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="OO328_000367"
     tRNA            complement(27654..27730)
                     /locus_tag="OO328_000367"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="OO328_000368"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="OO328_000368"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5929062.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="OO328_000369"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="OO328_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="OO328_000370"
     tRNA            complement(31514..31600)
                     /locus_tag="OO328_000370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO328_000371"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO328_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5929063.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO328_000372"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO328_000372"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5929064.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO328_000373"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO328_000373"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5929065.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO328_000374"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO328_000374"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5929066.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO328_000375"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO328_000375"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5929067.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="OO328_000376"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="OO328_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5929068.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO328_000377"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO328_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5929069.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="OO328_000378"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="OO328_000378"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5929070.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO328_000379"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO328_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5929071.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="OO328_000380"
     CDS             complement(41212..42177)
                     /locus_tag="OO328_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5929072.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO328_000381"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO328_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5929073.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO328_000382"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO328_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5929074.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="OO328_000383"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="OO328_000383"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5929075.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="OO328_000384"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="OO328_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5929076.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO328_000385"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO328_000385"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5929077.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO328_000386"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO328_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5929078.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO328_000387"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO328_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5929079.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO328_000388"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO328_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5929080.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO328_000389"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO328_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5929081.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO328_000390"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO328_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5929082.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO328_000391"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO328_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5929083.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="OO328_000392"
     CDS             49715..50680
                     /locus_tag="OO328_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5929084.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="OO328_000393"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="OO328_000393"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5929085.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="OO328_000394"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="OO328_000394"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5929086.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="OO328_000395"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="OO328_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5929087.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="OO328_000396"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="OO328_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5929088.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="OO328_000397"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="OO328_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5929089.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="OO328_000398"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="OO328_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5929090.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="OO328_000399"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="OO328_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5929091.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="OO328_000400"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="OO328_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5929092.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="OO328_000401"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="OO328_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5929093.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="OO328_000402"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="OO328_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5929094.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="OO328_000403"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="OO328_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5929095.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OO328_000404"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OO328_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OO328_000405"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OO328_000405"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5929096.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OO328_000406"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OO328_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5929097.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="OO328_000407"
     CDS             complement(62547..63476)
                     /locus_tag="OO328_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5929098.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="OO328_000408"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="OO328_000408"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5929099.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="OO328_000409"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="OO328_000409"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5929100.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..>70545
                     /locus_tag="OO328_000410"
     CDS             70226..>70545
                     /locus_tag="OO328_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929101.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVS"
BASE COUNT        17269 a        19140 c        17525 g        16611 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtc
//
LOCUS       DAJTRN010000006        67426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRN010000006 DAJTRN010000000
VERSION     DAJTRN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..2380)
                     /gene="glgP"
                     /locus_tag="OO328_000411"
     CDS             complement(<1..2380)
                     /gene="glgP"
                     /locus_tag="OO328_000411"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5929102.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGY"
     gene            complement(2399..3832)
                     /gene="glgA"
                     /locus_tag="OO328_000412"
     CDS             complement(2399..3832)
                     /gene="glgA"
                     /locus_tag="OO328_000412"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5929103.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3832..5127)
                     /gene="glgC"
                     /locus_tag="OO328_000413"
     CDS             complement(3832..5127)
                     /gene="glgC"
                     /locus_tag="OO328_000413"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5929104.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5145..7118)
                     /gene="glgX"
                     /locus_tag="OO328_000414"
     CDS             complement(5145..7118)
                     /gene="glgX"
                     /locus_tag="OO328_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5929105.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7115..9301)
                     /gene="glgB"
                     /locus_tag="OO328_000415"
     CDS             complement(7115..9301)
                     /gene="glgB"
                     /locus_tag="OO328_000415"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5929106.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9574..10677)
                     /gene="asd"
                     /locus_tag="OO328_000416"
     CDS             complement(9574..10677)
                     /gene="asd"
                     /locus_tag="OO328_000416"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5929107.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10869..11462
                     /gene="yhgN"
                     /locus_tag="OO328_000417"
     CDS             10869..11462
                     /gene="yhgN"
                     /locus_tag="OO328_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5929108.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11519..12859)
                     /gene="gntU"
                     /locus_tag="OO328_000418"
     CDS             complement(11519..12859)
                     /gene="gntU"
                     /locus_tag="OO328_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5929109.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12863..13390)
                     /gene="gntK"
                     /locus_tag="OO328_000419"
     CDS             complement(12863..13390)
                     /gene="gntK"
                     /locus_tag="OO328_000419"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5929110.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13529..14524)
                     /gene="gntR"
                     /locus_tag="OO328_000420"
     CDS             complement(13529..14524)
                     /gene="gntR"
                     /locus_tag="OO328_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5929111.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14748..15443)
                     /gene="yhhW"
                     /locus_tag="OO328_000421"
     CDS             complement(14748..15443)
                     /gene="yhhW"
                     /locus_tag="OO328_000421"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5929112.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15566..16603)
                     /locus_tag="OO328_000422"
     CDS             complement(15566..16603)
                     /locus_tag="OO328_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5929113.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16936..17424
                     /gene="yhhY"
                     /locus_tag="OO328_000423"
     CDS             16936..17424
                     /gene="yhhY"
                     /locus_tag="OO328_000423"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5929114.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17483..19225)
                     /gene="ggt"
                     /locus_tag="OO328_000424"
     CDS             complement(17483..19225)
                     /gene="ggt"
                     /locus_tag="OO328_000424"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5929115.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19345..19785
                     /locus_tag="OO328_000425"
     CDS             19345..19785
                     /locus_tag="OO328_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5929116.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19772..20515)
                     /gene="ugpQ"
                     /locus_tag="OO328_000426"
     CDS             complement(19772..20515)
                     /gene="ugpQ"
                     /locus_tag="OO328_000426"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5929117.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20512..21502)
                     /locus_tag="OO328_000427"
                     /pseudo
     CDS             complement(20512..21502)
                     /locus_tag="OO328_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21504..22349)
                     /gene="ugpE"
                     /locus_tag="OO328_000428"
     CDS             complement(21504..22349)
                     /gene="ugpE"
                     /locus_tag="OO328_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5929118.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22346..23233)
                     /gene="ugpA"
                     /locus_tag="OO328_000429"
     CDS             complement(22346..23233)
                     /gene="ugpA"
                     /locus_tag="OO328_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5929119.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23331..24647)
                     /gene="ugpB"
                     /locus_tag="OO328_000430"
     CDS             complement(23331..24647)
                     /gene="ugpB"
                     /locus_tag="OO328_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5929120.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25044..25757)
                     /gene="livF"
                     /locus_tag="OO328_000431"
     CDS             complement(25044..25757)
                     /gene="livF"
                     /locus_tag="OO328_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5929121.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25759..26526)
                     /gene="livG"
                     /locus_tag="OO328_000432"
     CDS             complement(25759..26526)
                     /gene="livG"
                     /locus_tag="OO328_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5929122.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26523..27800)
                     /gene="livM"
                     /locus_tag="OO328_000433"
     CDS             complement(26523..27800)
                     /gene="livM"
                     /locus_tag="OO328_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5929123.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27797..28723)
                     /gene="livH"
                     /locus_tag="OO328_000434"
     CDS             complement(27797..28723)
                     /gene="livH"
                     /locus_tag="OO328_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5929124.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28771..29880)
                     /gene="livK"
                     /locus_tag="OO328_000435"
     CDS             complement(28771..29880)
                     /gene="livK"
                     /locus_tag="OO328_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5929125.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30304..30687
                     /gene="panM"
                     /locus_tag="OO328_000436"
     CDS             30304..30687
                     /gene="panM"
                     /locus_tag="OO328_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5929126.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30886..31989)
                     /gene="livJ"
                     /locus_tag="OO328_000437"
     CDS             complement(30886..31989)
                     /gene="livJ"
                     /locus_tag="OO328_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5929127.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32260..33114)
                     /gene="rpoH"
                     /locus_tag="OO328_000438"
     CDS             complement(32260..33114)
                     /gene="rpoH"
                     /locus_tag="OO328_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5929128.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33359..34417)
                     /gene="ftsX"
                     /locus_tag="OO328_000439"
     CDS             complement(33359..34417)
                     /gene="ftsX"
                     /locus_tag="OO328_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5929129.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34410..35078)
                     /gene="ftsE"
                     /locus_tag="OO328_000440"
     CDS             complement(34410..35078)
                     /gene="ftsE"
                     /locus_tag="OO328_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5929130.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35081..36580)
                     /gene="ftsY"
                     /locus_tag="OO328_000441"
     CDS             complement(35081..36580)
                     /gene="ftsY"
                     /locus_tag="OO328_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5929131.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEEAAKEEITDEELEAQA
                     LAAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFIS
                     LFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEE
                     MGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTF
                     RAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNK
                     SHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKL
                     DGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36730..37326
                     /gene="rsmD"
                     /locus_tag="OO328_000442"
     CDS             36730..37326
                     /gene="rsmD"
                     /locus_tag="OO328_000442"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5929132.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37316..37585
                     /locus_tag="OO328_000443"
     CDS             37316..37585
                     /locus_tag="OO328_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5929133.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37588..37947)
                     /locus_tag="OO328_000444"
     CDS             complement(37588..37947)
                     /locus_tag="OO328_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5929134.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38088..38714
                     /locus_tag="OO328_000445"
     CDS             38088..38714
                     /locus_tag="OO328_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5929135.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38788..40986
                     /gene="zntA"
                     /locus_tag="OO328_000446"
     CDS             38788..40986
                     /gene="zntA"
                     /locus_tag="OO328_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5929136.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41088..41333)
                     /gene="tusA"
                     /locus_tag="OO328_000447"
     CDS             complement(41088..41333)
                     /gene="tusA"
                     /locus_tag="OO328_000447"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5929137.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41554..42219
                     /locus_tag="OO328_000448"
     CDS             41554..42219
                     /locus_tag="OO328_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5929138.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42292..42849
                     /gene="dcrB"
                     /locus_tag="OO328_000449"
     CDS             42292..42849
                     /gene="dcrB"
                     /locus_tag="OO328_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5929139.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42853..44070)
                     /locus_tag="OO328_000450"
     CDS             complement(42853..44070)
                     /locus_tag="OO328_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5929140.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44204..45253
                     /locus_tag="OO328_000451"
     CDS             44204..45253
                     /locus_tag="OO328_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5929141.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45308..45895
                     /gene="acpT"
                     /locus_tag="OO328_000452"
     CDS             45308..45895
                     /gene="acpT"
                     /locus_tag="OO328_000452"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5929142.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46006..47580
                     /gene="nikA"
                     /locus_tag="OO328_000453"
     CDS             46006..47580
                     /gene="nikA"
                     /locus_tag="OO328_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5929143.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47580..48524
                     /gene="nikB"
                     /locus_tag="OO328_000454"
     CDS             47580..48524
                     /gene="nikB"
                     /locus_tag="OO328_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5929144.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48521..49354
                     /gene="nikC"
                     /locus_tag="OO328_000455"
     CDS             48521..49354
                     /gene="nikC"
                     /locus_tag="OO328_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5929145.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49354..50118
                     /gene="nikD"
                     /locus_tag="OO328_000456"
     CDS             49354..50118
                     /gene="nikD"
                     /locus_tag="OO328_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5929146.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50115..50921
                     /gene="nikE"
                     /locus_tag="OO328_000457"
     CDS             50115..50921
                     /gene="nikE"
                     /locus_tag="OO328_000457"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5929147.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50927..51328
                     /gene="nikR"
                     /locus_tag="OO328_000458"
     CDS             50927..51328
                     /gene="nikR"
                     /locus_tag="OO328_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5929148.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51448..51807)
                     /locus_tag="OO328_000459"
     CDS             complement(51448..51807)
                     /locus_tag="OO328_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5929149.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51804..52079)
                     /locus_tag="OO328_000460"
     CDS             complement(51804..52079)
                     /locus_tag="OO328_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5929150.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52138..53262)
                     /locus_tag="OO328_000461"
     CDS             complement(52138..53262)
                     /locus_tag="OO328_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5929151.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53262..55997)
                     /gene="rbbA"
                     /locus_tag="OO328_000462"
     CDS             complement(53262..55997)
                     /gene="rbbA"
                     /locus_tag="OO328_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5929152.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(55994..57061)
                     /locus_tag="OO328_000463"
     CDS             complement(55994..57061)
                     /locus_tag="OO328_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5929153.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57427..59049)
                     /locus_tag="OO328_000464"
     CDS             complement(57427..59049)
                     /locus_tag="OO328_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5929154.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59311..60901)
                     /locus_tag="OO328_000465"
                     /pseudo
     CDS             complement(59311..60901)
                     /locus_tag="OO328_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            61285..62316
                     /locus_tag="OO328_000466"
                     /pseudo
     CDS             61285..62316
                     /locus_tag="OO328_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62403..62879)
                     /locus_tag="OO328_000467"
     CDS             complement(62403..62879)
                     /locus_tag="OO328_000467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929155.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62872..63636)
                     /locus_tag="OO328_000468"
     CDS             complement(62872..63636)
                     /locus_tag="OO328_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5929156.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63882..64643
                     /locus_tag="OO328_000469"
                     /pseudo
     CDS             63882..64643
                     /locus_tag="OO328_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64750..65250)
                     /locus_tag="OO328_000470"
     CDS             complement(64750..65250)
                     /locus_tag="OO328_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929157.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65401..66549)
                     /locus_tag="OO328_000471"
     CDS             complement(65401..66549)
                     /locus_tag="OO328_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5929158.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
     gene            66596..67426
                     /locus_tag="OO328_000472"
                     /pseudo
     CDS             66596..67426
                     /locus_tag="OO328_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        16379 a        18082 c        16577 g        16388 t
ORIGIN      
        1 agtagcccat attggcaatg ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt
       61 agagttcgtc gactttatcc tgacaatcga catagctgcg ataatccgcc agtacctggt
      121 agtgatcgcc gaagttgatc agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg
      181 gactgaatac accgctgccg atttgcgtca gcacctgatg cagctcctca tctttctcgt
      241 agtattcacg cggtttgtag ccctgacgac gcagttcttc cacttcttcc gctgtgttac
      301 caaaaataaa gatattgtca gcaccgacat gatccagcat ctcgacattc gcaccgtcca
      361 acgtaccgat agtcagcgca ccgttaagcg caaacttcat gttactggtg ccggaagctt
      421 ccgtccctgc cagcgaaatc tgttcagaca gatctgccgc cggaatgatc aactgcgcca
      481 ggctaacgct gtagttcggg atgaacacga ctttcagctt atcgccaatc tgcggatcgt
      541 tgttgatcac tttcgctacg tcattgatca aatgaataat gtgcttcgcc atgtaatagg
      601 ccgaagccgc cttaccgcca aaaatattca cgcgcggtac ccacttcgca tccgggtcgg
      661 ccttgatgcg gttatagcgg gtaatcacat gcaacacatt catcaattga cgtttgtatt
      721 cgtgaatacg tttgatttgt acatcgaaca acgcctttgg attcaccacc acattcagct
      781 gctgggcgat atactctgcc agacgctttt tgttctccag cttcgcctga tgcacagcgt
      841 gattaaccat tgggaaatca cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc
      901 gccagttacg gcccaggtgt tcgtccagca cggctgaaag cgatgggttc gctaccgcca
      961 gccagcgacg cggcgtcaca ccgttggtga cgttggtgaa acgacccggg aagattttcg
     1021 caaagtcggc aaacaacgat tgcaccatca gattagagtg cagttccgat acaccgttaa
     1081 ctttgtggct cacaacaacc gccagccagg ccatacgcac acgacgaccg ttggattcat
     1141 caatgatcga cgcccgtccc agcagatcgg tatcgttcgg atactgttcc tgcaaggttt
     1201 tcaggaaata gtcgttgatt tcaaagatga tctgcaggtg acgcggcaga attttaccca
     1261 gcatatcaac cggccaggtt tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga
     1321 agacctgaca acacacctca aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca
     1381 gacgcatcat ctcaggaatc gacagtaccg gatgggtatc attgagatga atcgcgattt
     1441 tatccgccag gttatcgtag gttttatgca actgataatg acggcttaaa atgtcctgaa
     1501 tggtcgagga aaccaagaag tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg
     1561 agtcatccgg atacagtacg cgagatacgt tctcggagtg gtttttatct tccactgccg
     1621 cgaagtagtc accctggttg aatttaccga ggttaatttc gctactggct tgcgcactcc
     1681 acaaacgcag cgtgttggtc gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga
     1741 ctcccagaat ctcttcggtt tcaatccagc gcgttttttt accttcctgc tgaatgcgac
     1801 caccaaaacg gactttatag cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt
     1861 ccagccagta gtctggcgac tctttctggc taccgttaac gatgttctgc ttgaacatac
     1921 cgtagtcata gcggatgccg taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa
     1981 ggaagcaagc cgccagacgt cccaggccac cgttaccgag gcctgggtca ttttcttcat
     2041 caatcagctc ttcgagattt aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa
     2101 ttcctagcga caacatggcg ttggagagcg tacggccaat caaaaactcc atcgacaggt
     2161 agtaaacctg acgagtttct tgcgacaact gggcacggtt tgaacgtaac cagcgctcca
     2221 cgagacgatc gcgcacagca aataacgttg cgttcagcca ttcatgttta ttggcgacga
     2281 ccgggtcctt tccaatcgta aacatcagct tgtaagcgat agagtgctta agagcttcta
     2341 cgctaagcgt gggcgatgaa tatgtaaacg gagcattcat ataggcgttt cctgaaaact
     2401 atttcaagcg atagtaaagc tcacggtacg acttcgccgc gacctgccag ctaaaatcca
     2461 ttgccatagc ctgacgttgc acaaaccgcc acagtgaagg acgggaccac agtacaaaag
     2521 cacgccgaat agcccgtaac agcgaccagg cattactatc ttcaaagaca aacccactgg
     2581 cgacgccatc tgcaaggttc tcaagagaac agtcagaaac cgtatcagca agcccaccgg
     2641 tgcgccgcac taacggcagc gtaccgtact tcaatccata aagttgcgtt aagccgcacg
     2701 gttcaaaacg gctgggcacc agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg
     2761 cttcgtgata gccaatctga acgcccacct gaccggggta ttccgctgcc gccgcaagga
     2821 aaccttcctg cagcaccgga tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca
     2881 gaagccccgg taaggcttcc agcaccaggt cgagaccttt ctggctggtc agacggctca
     2941 ccactgcaaa aagcggcact ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc
     3001 tcttattttc cgctttatct tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt
     3061 ccgtctctgg actccagatt ttctcgtcca cgccgttcag tacgcctgaa agacgccctt
     3121 cacggtgacg ctgttgcaac agaccttcca taccgtaggc aaactgcggt tcggtgatct
     3181 cgcgagcgta ggttggactg accgccgtaa tgtgatcggc atagtacaga ccggccttca
     3241 ggaaagagat ttgtccgttg aattccagcc catgaatatt aaagaatgac catggcaatt
     3301 ggatgtcatt catgtgatgt gcataaaaca tgccttgata ggccaggttg tgcacagtaa
     3361 acaccgactt cgcaggacgc ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc
     3421 agtcgtgcac atgcaccaca tcaggacgcc agaatgggtc aagcccgctg gccatttctg
     3481 ccccaaccca ccccagcagc gcaaaacgca atacgttgtc ggtataggca aataagttgg
     3541 tatcgtgata cgggcttccc ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc
     3601 caaccccgtt gtaatgaccg aacaacagcg tgatatgccc ggcgaaagta tcacgacggg
     3661 atactacctg cgcatcggtc acgccacggc gaatatcggg aaatgcaggc aacagtacgc
     3721 gagcgtcaac gccgtctgcg atttgtgctg cgggtaatgc cccaataaca tcagccagac
     3781 cgccggtttt aagcagcggg aacatctctg aacatacatg taaaacctgc attatcgctc
     3841 ctgtttatgc cctaacttcc gtagcatttc gcgcgttacc agcacgatgc cttcttctga
     3901 acgatagaaa cgacgtgcat cttcctctgc gttttcacca atcaccatgc cttccgggat
     3961 aacacaagca cgatcgatga cgcagcggcg cagacggcac gagcgaccta cccatacttc
     4021 cggtaacaat acggcggaat caatgttgca gaatgaattc acgcgaacgc gcgagaacag
     4081 aacggactgc accaccaccg aaccggagat cacacaaccg ccggatacca gtgagttaag
     4141 ggtcatcccg tggctaccgg agcgatcctg cacgaatttc gctggcggta atgattcatt
     4201 gtaggtgcga attggccaat tgcgatcgta catatccagt tccggcacca cagaggccag
     4261 atcgagattc gctttccagt aagcttccag cgtacccaca tcgcgccagt acggctcggc
     4321 atccgggtcg gattgtacgc aagagagcgg gaacgggtgc gcataggcca gaccggcttc
     4381 ggtgatcttg ggaatcaaat ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc
     4441 ttcttccagc agttcataca gatagtcggc gtcaaagacg tagataccca tactcgccag
     4501 agatttgctc ggatcgttcg gcattgacgg tgggttagca ggtttttcaa cgaattcgat
     4561 aattttatcg ttctcatcaa ccgccataac gccaaatgcg gaggcttctt caatcggtac
     4621 tggcatacaa gcaacggtgc aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga
     4681 gtagtcttgc ttgtagatat ggtcgcccgc caggatcacc acgtattccg ctttataacg
     4741 gcggataatg tcgaggtttt gggtgaccgc atctgcggtg ccgcgatacc agttttcccc
     4801 tttcattctc tgctgtgctg gcagcagatc gacaaactcg ttcatttctt cattgaagaa
     4861 tgaccagccg cgctgaatgt gctgcaccag agtgtgggac tggtactggg tgatcacgcc
     4921 catacgacgg atcccagagt tgatgcagtt agacagcgca aagtcaataa tgcggaactt
     4981 accgccgaag tgtacggccg gttttgctcg cttattggtt aaatccttca ggcgggtacc
     5041 acgtcctccc gccagtatca gggcaacaga tttcaatggc agctggcgcg ccaacattaa
     5101 gtgatcgttc ttctctaaac taaccatgac taactccttt tttatcatct ctggaacaca
     5161 cacaatccgt gtgcaggtcc ctgccagaca accgtaatca ctgggttatc ctctccagcg
     5221 aatgggggaa tggcgtgcca ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg
     5281 gcgttaattg cgatcaaaaa gcgatccgag agcagaattt gcagctgttt cggcccgttt
     5341 tgccactcat ccgtgcttaa aggttgagca tatcgattta gccaacggac attgccgtca
     5401 ccttcttccc accagcgatt ctccaccaaa gcgggaatgc gcttgcgcag atggattaac
     5461 gcggcggtaa atgcggttaa accactgctt gcctgcgacc agtccaacca gtttaattgg
     5521 ttatcctgac agtaggcatt gttattgcca tgctggctgt gaccatgttc gtcaccggcc
     5581 agtaacatcg gcgtgccctg ggagagcaac aacgttgtta acagggcatg aatgctgtcg
     5641 cgccgccgtt caaccaggtc aagagtaccg cctaaccctt ctttaccatg attgttactg
     5701 taattgttgt tggtcccgtc gcgattttct tctccgtttg cttcattgtg tttatggttg
     5761 aagcaaacgc agtcgcgaag cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca
     5821 ctcggcagac gaccattacg tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac
     5881 gcccccagag gcaaatcata atgtagccag aaacgacggg cagcatcgcg gaaatgatcg
     5941 ttccactcgg caaacagcgg cgggaaattt cccacctgat aaccactagg agcgatatcc
     6001 cacggttcag caattaactt cacctgcgag agcaccgggc agttctggat agcggtaaac
     6061 aacggcgcat cctgacggaa ctctggcgta cggcccatga ctgccgccag atcaaagcgg
     6121 aaaccatcga cgtggcaggt ttctacccaa taacgcaggc aggcgctggc ataatccacc
     6181 accgccggat gactcaaatt gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg
     6241 tcttctctta tccaataata gctacggtta tcgatcccac gcagcgagaa taacgggccg
     6301 tcgaggtcca gttccgcact atggttgagc acgatatcaa gaatgacttc gatacccgct
     6361 ttatgcagtg ctttgattgc atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca
     6421 tacgccggat gcagcgcaaa catcgccacc gggttgtaac cccagtagtt acttagcccc
     6481 atgcgttgca gacgtggttc actggcaaac tgcgccactg gcagcagttc cagcgcggta
     6541 atgcccaatt gtttcaaata gttgatcatc accggatgcc cgagggcttt ataagtgcca
     6601 cggatctcga ccgggatctc cgggtgcaag tacgttaatc ctttgacatg ggcttcataa
     6661 atgatggtgc tgccccacgg cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca
     6721 accaccacta cgcatttcgg cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga
     6781 ccggcgtgca gcagcgggtt atctttaaac tccccgtcaa tttgccgcgc gcaaggatca
     6841 atcaacaact tcgccgggtt aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg
     6901 cgataaccat aacgcaaacc cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca
     6961 ctgttccctg gcaagtcata gcgatgttcc tggccattgg cgtcaaagac acacagttct
     7021 acccgctcgg catgagcgga gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg
     7081 ccgaggggag cgggtttgcc aatggcgagt tgtgtcattc tgcctcccga accagccaga
     7141 tagtggccag cggtggtagc gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct
     7201 catcgctgtg taccgtgccg ccattgcctg cattactgcc gtgatagtgt atggaatcgg
     7261 tattgaggat ttcacgccat ttacctggct gattaatgcc gaagcgataa tcatgacgcg
     7321 gcaccggcgt aaagttactg gcaacgataa tttcgttacc ctctttatcg cgacgcacaa
     7381 agatcagcac cgagcgttct ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa
     7441 aatccagttc atgcattgct ttatggtggc ggtaggtgag gttcagatcg cgcaccagac
     7501 gctggacacc gtggtgccag ttatcgccgc cttccaacag atgccagtcg aggctggcgt
     7561 catggttcca ctcgcggccc tgggcaaatt cgttccccat gaacagcagt ttcttgcccg
     7621 gaaatgccca catccagcca tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc
     7681 ccggcatgcg gtcgagaatc gattttttac cgtggaccac ttcatcatgc gacaacggca
     7741 ggacgaagtt ttcagtgtag ttgtagagca tcccgaaggt cagtttatcg tgatgatact
     7801 gacgataaat cgggtcgagc ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc
     7861 acttgtacca gaagcccaga ccgcccatat cctgcggacg agaaacgcca gggaaatcgg
     7921 tagactcctc agccattgtc accgcaccgg aaacctgctc accaagaata cggttggtat
     7981 tacgcaagaa ttcaatcgct tcaagattct cgcgcccgcc aaattcgttc gggatccact
     8041 ccccctcttt acggctgtag tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg
     8101 catcaatacc aaaacgttca atccagtaaa gcgcgttacc gacgaggaag ttactgactt
     8161 cacggcgacc atagttgtag atcagcgtgt tccagtcctg atgatagcct tcacgcggat
     8221 cgctgtgttc atacaagttc gtgccatcaa attcggcaag cgcaaagtca tcagtcggga
     8281 agtggcctgg cacccagtcg agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga
     8341 aataacggaa gtcgtcgcga gtaccaaaac ggcgggttgg tgcatacagg ccggttggct
     8401 gataacccca actgccatcg aagggatgct cgttaatggg cagtagttcg aggtgggtaa
     8461 agcccatcca tttagcataa ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga
     8521 aattgttgtc ggtgtggcga cgccaggaac ccaggtgaac ttcataaata gagattggcg
     8581 catcaaactg attcgctttt ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc
     8641 aaataagaga cgcggtttcc gggcgcattt gcgcttcgaa ggcataaggg tcggacttca
     8701 gacgcaagtt gccattggca tcaatcatct cgtatttata gagctgaccg ttatgcgccc
     8761 cagggataaa cagttcccag atgccgctct ctttacgcag gcgcatcggg tgacggcgac
     8821 cgtcccagta gttgaattgc ccaaccaccg agacccgacg ggcgtttgga gcccagacag
     8881 agaaacgcgt acctgtgacg ccatccatag tatctgcatg cgcgcctaag gtttcatacg
     8941 ggcgcaggtg agtaccttca gataatagcc aggcatccat ttcctggatt agcggaccaa
     9001 aacggtaagg atcatcaatc aggttttgct gaccatgcca gacaacagcc aactgatagc
     9061 ggaaaaaatt cttacgtcgc ggaatgacgc cgctaaagaa tccccgtgag tcgagacact
     9121 ccagttttgc gagtttgcgc ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg
     9181 gtaaaagggc acggacttcc agtcccgcgg tggttttatg cattcccagt acggaaaaag
     9241 gatccgcaaa atggcctgca attagcgcgt taatcacgtc tctatcgata cgatcggaca
     9301 tgcttgtctt cctgttttat tgtgtcaccc catccaattg gatttttgac ttctggttgt
     9361 gacatttttt tgacctgaac ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact
     9421 tcaggtaagg ctgtgaatac tcatgtattc agccaccctt aaagaatagc caatgctcta
     9481 tttaactccc ggtaaatcat gaaacatctg cgcttactcc tgtattacgc actaacaggg
     9541 gcggcatcgc gccccagatt taatgaataa agattacgcc agttgacgaa gcatccgacg
     9601 cagcggctcc gcggcccccc acagcagctg gtcgcccacg gtaaaggctg acaggaactc
     9661 tggtcccata ttcagcttac gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc
     9721 agctggggtt agctcacgca tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg
     9781 attgtgcgca gccagcagtt cttcgacggt cggaatagac acatcttttt tcaatttaat
     9841 agtgaatgcc tggctgtggc agcgcaatgc cccgacacgc acacataaac catctaccgg
     9901 aattacggaa gatgtgttga ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg
     9961 gctctgaccg ttatcgagct gtttgtcgat ccacggaatc aggctacccg ccagcggcac
    10021 gccaaagtta tccaccggca gctcaccgct acgggttaag gttgtgactt tgcgttcgat
    10081 atcgagaata gcagaggacg gggtcgcgag ttcatctgcc acatggccat acagatggcc
    10141 catctgggtt aataactcac gcatatgtcg cgcaccaccg ccggaagcgg cttggtaggt
    10201 tgcaacggac acccaatcaa caagatcatt ggcgaataaa ccacccaacg acatcaacat
    10261 caggcttacg gtacagttac cgccaacaaa agtcctgatg ccattattta atccgtcggt
    10321 aatgacgtcc tgattgacgg ggtcaagaat gatgatggcg tcatctttca tgcgcagaga
    10381 cgatgctgcg tcaatccagt aaccttgcca tccgctttca cgaagctttg gatagatttc
    10441 gttggtataa tcgccgccct gacaggtcac aatgatatcg agggccttta gcgcctctag
    10501 atcaaaggca tcctgaagtg tgccagtggt tccgccaaaa gacggcgcag cctggccaag
    10561 ctgagaagta gaaaagaaga cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg
    10621 ttgcatgaga acggagccga ccataccgcg ccagccgata aaaccaacat ttttcataag
    10681 cgttttttcc tgcaaagatg tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt
    10741 tcaccataca aaaagctgcc aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc
    10801 agtaatgcga cttatcctgc tttgttagca cgcagaaagt ccgcggcaat tatcagggaa
    10861 tttgagttat gaatgaaatc atttctgcag cagttttatt gatcctgatt atggatccgc
    10921 tcggaaacct gcctattttc atgtccgtac tgaaacatac tgaaccgaaa agacggcggg
    10981 caatcatggt gcgagagttg cttattgctc tcctggtgat gctggtgttc ctgtttgcgg
    11041 gtgagaaaat tctggcattt ctcagcctgc gagcagaaac cgtctccatt tctggcggca
    11101 tcattctgtt tctaatcgcc attaagatga ttttccccag cgcttcagga aatagcagcg
    11161 ggcttccggc aggtgaagag ccatttatcg tgccgttggc aattccgtta gtcgccgggc
    11221 cgactattct cgccacgctg atgttgttgt ctcatcagta cccgaatcag atgggacatc
    11281 tggtgattgc tctgctgctg gcctggggcg gcacctttgt catcctgcta cagtcttcgc
    11341 tatttttacg tctgctgggc gagaaagggg tgaacgcact tgaacgcctg atgggattga
    11401 ttctggtgat gatggcaact cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt
    11461 aacgaggaga gtttttcgaa aagagcccgg tagcgtcaca actaccgggc gaacaaactt
    11521 aactcaacag ctggaacgca atcatcccaa cgatggcacc aacagtgccg aggatggttt
    11581 ccatcatggt ccaggttttc agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca
    11641 accagaaacc agcgtcgtta acgtggctga caacaatcga accaccagcg atacaaatcg
    11701 acagtgccgc catttgcgca ccagagtagt tcagttgttc aatgactggc atcaccagtc
    11761 ccaccgccgt taaacaggct acggtggcag ccccctgaat gatgcgcact gcagcagcca
    11821 gcacgaagca ggtgatagca atcggcaggc ccatgccggt taacgcttcg cccagtgccg
    11881 gacctacgcc agagtcaacc agcacctgtt tgaacacgcc gcctgcacca atcaccagca
    11941 gaatgatccc cgccggttgc agcgcgtgac cgcaaatctc catcactttg tcttttggca
    12001 tgccctgacg cattgccagg ccataaatcg ctaccagaca agcaaccaga atcgcggtaa
    12061 acggatgacc aataaactcg aaccattcgt aagcggtaga gccttctggc acaaaacgcg
    12121 cggcaatggt tttcagcccc accagcacca gcggcaacag gatcagcgac aggctgaatc
    12181 cgaaagatgg cattttacct tcgccgagat gcggttcgct gatgtcgtca ggaatatgca
    12241 gctcaacgta acggctgatg aagttacccc acagcggccc ggcaataatc attcccggaa
    12301 ttgccgcaca caggccaatc aggatcatcc agccaaagtc ggcattcatc tgcgatgcca
    12361 gcagcattgg cgctggccca ggcaccagga acgctgccgc ggccgccacg cctgcgaata
    12421 atgggattac cagcttcacc aggttcgtac cggtgtggcg cgccattgag aaagcaacgc
    12481 taatcagcag aacaatcgcc acttcaaaga acagcggtag cgcacagacc agccccgcaa
    12541 ggccgatggc ataatgcgcg cggctgtgac cgaaggattt gagcattttg acggcaatct
    12601 gatcgactgc gccggtttca tataagatct tgccaaacat agctcccagg gcgacaacca
    12661 ccgccaggaa gccgagggtg cctcccatcc ctttttccat cgtcgctgcg attttatcga
    12721 gcggcatacc ggaaaaaagg ccagccccca tggacaccac catcaaagcc aggaaagcgt
    12781 gcatacgcgc cttcatgact aaaaacagca gcagtaaaac agaccctact gctgttaaaa
    12841 caagcgttaa tgtagtcact acttatttgc cttttttaat aacctcaatg gtgcttgcca
    12901 caacaccttc cagcggttga tcgatatcca ccaccagtac atcggtttcg tccgcacccg
    12961 gctcctgcag cgtttcaaac tgcgtcacca acatttgggt tttaaagaaa tggcctttgc
    13021 gcgctttcag gcgactttca atcacatcaa aatcgccttt caggtagatg aaagagagat
    13081 tcggattacc ttcacgcagc aagtcgcgat agtgcttttt cagtgcagaa caggcgatca
    13141 gcgacacttt attggtgcgc tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg
    13201 gtttgcgatc gtcgtcattc agcggttcgc cagacgccat tttttcgata ttgcgccgtg
    13261 gatggaggaa atcgccatca agaaacgcgg catgaagttg atgcgccact tcactggcga
    13321 ccgcagattt gccgctgccc gatacgccca tcaagacgta aatgtggtga tcatggttag
    13381 tcgtgctcaa agtggtgccc ccacaataca agaattaaaa atgttacggg taactgttat
    13441 cggcaacatt gtccagccgg acaatatcag aagcaatatc catccgtgcc ttaagtgtat
    13501 aagtgtgagc tacttcaaat ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac
    13561 ctaaatctaa cattttcggt gtcacagatt cgccacgaat acgcgccagc aggcgttcag
    13621 cgccaatact gcccatccgc tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca
    13681 cctgaccaat gtcatgaccg tggaaaccgg caatcgccat atcgtcagga acttttaacc
    13741 ccagacgctg acattcaaac gccgcgccga ccgccagatc atcattagta cagaacacgc
    13801 catccagctg cggatattcc cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag
    13861 aagaagattg ctcaaccatc acgctatatg gcaccaggcc tgcatccagc atcgcctgtt
    13921 cgtatccctt ctgtttgatg atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt
    13981 ggcgatgccc acgagcaata atggcggtag tcatctggcg tgccgcttca aagttatcaa
    14041 aaccgacggc gatatcgagg cacggcgact ggctgtccat caactccacc accgggatcc
    14101 ccgccacttc aatcatcttt aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc
    14161 cgtcgatatt ccaggagagc atggattcga gacgttcttg ctccatttcc ggtttataac
    14221 cgtagtgcgc cagcatggtc tgataaccgt gcgcgtcggt gacgctttcg attccgcgta
    14281 atacttccgc gaaaacctgg ttggtgagag aaggtaacag gacgccaatc gcccggctgg
    14341 tagcgttaga gaggatatcg ggcgcacgat tgggaatata gcccagttca tcaagagcgg
    14401 cggcaatctt gccgcgtaga gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga
    14461 ccgtcatttt ggtcacgcct acacggtcag ccacatcctg aagtacgggt cttttctttt
    14521 tcatcgtcct gaaggtacaa aagagataga ttctcttagt ttaacacgga cgcatcagaa
    14581 ccttcccggg gaaaacagtg cttgtttaca attattagat atacactaaa tcaattgcat
    14641 cgcattgtgc taacgacgcg gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg
    14701 agcgaggaag cctgcaaaaa ggcaggcttc aaagatgccg ttgagtttta aaccggcggc
    14761 agatcgaaca gtaacacttc gctgtcgcta tccgcatgga tggagattgc ctgctcatcc
    14821 cagattgcca gaccatcgct ggtcgaggct ttcacgccgt taatggtaac attgcctttt
    14881 accacctgaa tccagacgcg gcgttcagcg gcaatctgat gcaccgactg ctcatctttc
    14941 agcaacgccc aacgatacag ttccatatcc tgatgcactt tcagcgaacc atctcgcgca
    15001 tccggcgaga gcaccagctg tttgccctgc acggcatcga agcgacgctg ttcataacgc
    15061 ggcgtaatgc cattttcttc aggcatgatc cagatctgat acagatgcag acgctcggtg
    15121 ctgcttgggt tgtactctga gtgacgaata cccgtacccg cactcataat ctggaactca
    15181 cccgccggaa cctgctcttt attgcccatg ctgtcctgat gctcaacagt accttccagc
    15241 acgtaggtca aaatttccat atctttatgc gggtgagtgc cgaagccctg ccctacttca
    15301 atcacgtcgt cgttaatcac gcgcagcgcg gagaagccca taaagttcgg atcgtaatag
    15361 ttggcacaag agaaagtatg ccaggagtcc agccagccat gatttgcatg accacgttca
    15421 tttgctttgc gtaagtagat catttgtatt cgccccctga atgatttcga tgggcttagt
    15481 gtggacccga tctgcctggg atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat
    15541 tgaactattc gaggggccat caaacttaat tcgcgagagt cactgtggag ggagaggctt
    15601 gctcaaatcc gcgttcaagg atttccagat tggtaagaac ttcagattcc ttgacgtaat
    15661 ttggcgcacc gtgggtgatg gtttgataca acgcatcata aacacgcccg taatcgccca
    15721 tctccggctt catctcttct ctgaccgtca caccctcgtc attgacatac tccagcacac
    15781 cgaccgaatc atccgctgcg aatcccggtt cgcccggcat aatattagcc ttcaggctgg
    15841 tttcctgctg gtcgataccg tatttaataa acgaaccttt cttaccgtga acgataaatt
    15901 tcggataatc gattttcacc agatggctgg ttttgacgat ggctttcagg tcgccataaa
    15961 acagttgcgc ttcaaaggtg tcgtcaggat tggctttatt acgcaggctg cggatgtcat
    16021 aagcgacgtg atccgggcga ccgaacagag aaataatctg gtccatcgta tgcacaccaa
    16081 ggccatagaa cgcgccatcc tgcggcagtc caggtttggt ttctgccacc gggcggtaat
    16141 agtcaaaatg gctttccact tcgacaatct ctcccagctt gccgctttca atcgcttttt
    16201 tcgccgtcag gaagcaggaa tcaaagcgac gattctgata tggcgtgacg gtcagccctt
    16261 tgcttttcgc cagtgcaaac agctctttcg cctgcgcaag tgtcggagtg aacgtttttt
    16321 cgaccagcac atttttcccg gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg
    16381 catgggtgca gacaacaacc aacttaacat cgggatcgtt tagcacttcg tcgagatcgc
    16441 tggtgaaatg gatatgagag taaatgggag cctgttcttc cggcttcgca tggcgacgaa
    16501 aaatatgcgc gacatgccag ctatccttgc ggttaagtac atacggcaga tggtaacggg
    16561 tggtgctttt gccgaagcca ataaaggcgc agttgatgac catgatttcg tcctttttaa
    16621 ggtggttatt tacaccttag cgcaaagcgg acgtggttcc tactggagtg cgcataacga
    16681 acacaagcac tcccgtggat aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg
    16741 ctggctaagt aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg
    16801 gtcttcctga tcgcgataca ataataatca ttctcattcg cacttgtcca acactttttg
    16861 caaaaaaatg catttgactc gcatttgaaa gtcaatgatg ttgaaaggga catttacccc
    16921 aaagaggaca aaggaatgag tgagatagta atacgccacg cagaaacacg ggattacgag
    16981 gccatcaggc agattcacgc ccagccggag gtgtattgca acacactaca ggtgcctcat
    17041 ccttccgatc atatgtggca ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc
    17101 gcctgtattg atggagacgt cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc
    17161 cgcagtcatg ttgccgattt tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc
    17221 gccagcgccc tgatgcgaga gatgattgaa atgtgcgaca actggttacg ggtagatcgc
    17281 attgaactaa ccgtgtttgt cgataacgcg ccagcaatta aggtctataa aaaattcggc
    17341 tttgagattg aagggaccgg taagaagtac gcattgcgta atggtgaata tgtcgatgcg
    17401 tattatatgg cgcgggtgaa atagagaatt tgcaatctaa gtttgtcatt taagccctca
    17461 cctaccggga gggcttaaga tattaatacc ccgccgttaa atcatccacc gagcgcgggt
    17521 cggatgcgcc gtacaactca ccgtccggcc caaccataat gctttgtgtg ctgcccatcg
    17581 cctctttcag cgccactttc tgaccttttg cttccagcag cttgagcgta tccgggctaa
    17641 accctttttc gacacgcagc tcgtccggca accactgatg gtggaaacgc ggcgcattgg
    17701 tcgcttcggc gacgttcatg ccataatcga tgctattcac caccatttgc agcaccgtgg
    17761 tgataatccg gctaccgcct gggctaccgg taaccagcca ggttttaccg tctttcacca
    17821 caatggtcgg cgacatcgac gacagcgggc gtttgttcgg cccgacggcg ttggcatcac
    17881 cgcccaccag cccgtaaacg ttcggtacgc ccggtttggc ggagaaatca tccatctggt
    17941 tattaagcag aataccgctc tcacccgcga caatacccgt accgaaggtg gtgttcagcg
    18001 tataggtcac cgccaccgcg ttaccgtctt tatccactac agagtaatgg gtagtttgat
    18061 tactctcata aggcgcaagc ttgccagggc gaatctcact ggacggcttc gcttttttga
    18121 tatcaatttg atcggcaatg gatttggcat aggctttatt ggtcagtgcc tgccacggga
    18181 ctttgacaaa atccgggtcg ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg
    18241 cttctgccat gatttgcatc gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt
    18301 tttccagaat attgaggatt tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg
    18361 agtaaacctg atacccgcga taatcgccgc ttatcggagt gcgttcgacc gctttatagg
    18421 ccgctaaatc ttctttagtg atcaagccac cgtttttctg catctcctgg gcgatctgtt
    18481 ccgcaatcgt gcctttatag aattcgtccg ggccgttttc agcaatcatc tccaggctct
    18541 ttgccaggtt cgcctgcacc agcgtgtcgc cctttttcag cggctcgccc tctttccaga
    18601 agatagcttt actgttttcg tgattcggca acacttcgct accgtaggtt ttgagatcgt
    18661 cagccagcgc gtcgttaacg ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga
    18721 ctttgttcag cggcatggtg ccgtatttat ccagcgccag cgagaaacct gctaccgtac
    18781 ccggtgtgcc ggaagccaga tgcgaagtga gtgatttttt gctgtccggg ttgccctgat
    18841 catcgaggaa catatcgcgg gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg
    18901 tggtattgcc atttttcgag cggattaaca taaaaccacc accgcccaga ttccctgcct
    18961 gcggatgcgt taccgccagc gcgtagccca ccgccacggc ggcatcaacg gcattcccgc
    19021 cctccttgag gatatccacc cccacctgag tggcagtggc gtccacagac gctaccattc
    19081 cctgtttcgc gcgtaccgga tggaagacat cttcctccac accatacgag acgggcggcg
    19141 caggaggcgc ggcggcggcg ctaaaacaac ttcctgagag cagagcagca atggccaccc
    19201 ggcgtaaaaa cgtcggtttt atcatcgtta ttctccagag attaaggggc aaccccagct
    19261 aagcctggta tataactctg aattaatcat cgttttgtcg ggaagcgagt aaacttaaag
    19321 gatatcttca gaggaggata cgcgatgaaa cgacttctga ttcttacggc actcctgccg
    19381 tttgtcggct ttgcacagcc cattaatact ctgaacaacc ctaaccagcc ggggtatcag
    19441 atacccagcc agcagcggat gcaaacccag atgcagactc agcaaatcca gcaaaaaggg
    19501 atgctgaatc agcaactgaa aacgcaaact cagttgcaac agcagcattt agaaaaccag
    19561 ataaacaata attctcagcg ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg
    19621 cagcaaatgc tgcccaacac caacggcggg atgttaaaca gcaaccgtaa tccggatagt
    19681 tcgttgaatc agcagcatat gctgccggag aggagaaaca gcgacatgct gaatcagccc
    19741 agcacgccgc agcctgacat tccgttgaaa actattgggc cgtaaagttc ggaccaatca
    19801 cgtcaatcgc atcggtacag atgcaatcca cgccccagcg cagcaactct gccgcgtgct
    19861 ggggtttatt gacggtataa accagaatcc gtaatccggc gtctttcaac tgcatcactc
    19921 gcgctttatc gagtaactta tgattgagat gaatagagac gcagcccagc cgcgcggtca
    19981 gttcgcgcca gtcgtcgcgc cactcatcaa gcaataaacc acgcggcagt tccggtgccg
    20041 cctgttgtgc agcttctaaa gcatcaatct caaacgatga cagcagcggc ggcgtcatac
    20101 cggcccacag ttggcgtgcc gccagcgcca ccattttgcc cgttaatggt ccagtgccag
    20161 tggtgggttt gatttcgata ttcgccatca tcccgtgttc gcgacagcgt tccgccacct
    20221 gcgaaagcaa cggcagcggc tcacctttaa acgctttgct gtaccaactg cccgcatcca
    20281 cgcgcagtaa atcctgccag ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt
    20341 cgagattgtc gtcatggagc aggaagatct cgccatcttt cgataacttc gcgtcaaatt
    20401 cgatcatctt atgaccgtat tttgccccga cgtcgattgc cgccagggtg ttttccgggg
    20461 ccagcttacc gccgccacga tgagcgacga tgcggggata aggccagtta ctcatactcg
    20521 ttgtcctgtt tcaccatcaa aaagatgcag ctgattttcc gccagatgca gccacagcgt
    20581 gctgcctgcc gtcgggcgct cctgatgcgc cagtcgcacc accagcttct gcttgcgagc
    20641 ttagcgcaat atgttccggg cgaatgccga gggtcatttt acgcccggca tactgacggt
    20701 agccaccgtt tagcggtagc acgataccgc cgtccagttc gaaatgcgtg ccttcattat
    20761 tcatgcggcc tgtcagcagg ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc
    20821 tggcgggctt ttcgtagact tccaccggcg tgccaatctg ttcggcaacg ccgccgttca
    20881 tcaccattac tcgctgggcg agcgtcatcg cctcaacctg atcgtgagta acgtagagtg
    20941 aagtcgtttt caggcgacgg tgcagctgtt gcagttcaag acgcatctgt acgcgcagct
    21001 tggcatcgag gttagagagt ggctcatcaa acaagaacac cgccggatcg cgcacaatcg
    21061 cgcgtcccat cgccacacgc tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga
    21121 gcagaccgtc cagctccaga atgcgcgccg cttctttaac gcgctcggct atttgctgct
    21181 tgcccatgcc gcgaattttc agcccccacg ccatgttttc ttcgacgctc atatgcggat
    21241 aaagcgcgta attctggaac accatcgcaa tcccgcgatc tttcggctcc atttcggtca
    21301 cgcgctggtc gttgatccag atatcgcctt cggtcaccct ctccagcccg gcaaccatac
    21361 gcagcagcgt cgatttcccg cagccagacg gcccgaccat cacgataaat tcgccatccg
    21421 ccacatcaag ggtcagcggt ttaatgacct aggttttgcc atcccagctt ttggttactg
    21481 cctgtaattt cagtcctgcc atcttatttc tcactatcga ccaggccgcg cacgaaggca
    21541 cgctgcatca ctaaaacaat cactactggt gggatcaggg ttaacaacat cgccgccatc
    21601 actgagttcc attccgtggt gccttcgcca gtggcgatca tccctttgat ccctgccacg
    21661 gtggtgccga gatccacgtc ggtaataatc aacaacggcc acaaatactg gttccagccg
    21721 taaataaagg tgatcacaaa cagcgccgcc agattagttt tggagagcgg aaaaacgatg
    21781 tcgcaaaaga agcgcattgg tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc
    21841 ggcagcgtca taaagaactg gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc
    21901 agcgttaaac cggcgtagct gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg
    21961 aagatacgta cttcaaccgg cagcatcagg gtgataaaaa tcatccagaa gaagaggtta
    22021 cgtagcggaa aacgaaacca gacaatggca aatgccgaga gcatcgagac agtaattttg
    22081 ccgagcgtaa tgctgaacgc catcacaaag ctgttaagca acatccgcca gaacggcgcg
    22141 ctatttgtgc ctaccccgtt cacccagatg ttgtggatgt tttccagcag atgcgtgccg
    22201 gggatgagcg tcatcggcgc ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac
    22261 gccacgtaca gcgggaagag gatcaccgcg atcccgagga tcagcatggt atggctgaat
    22321 atcgtcagcc acggacggtt ctcaatcatt ggtaacgcac cttgctttca acatagcgga
    22381 actgcaccac cgtcagcacg atgacgagga acatcagcac catcgactgt gcggcagacg
    22441 aagccagatc cagtccggta aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg
    22501 cctgaaccgg cccgccggac gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat
    22561 acaccaggtt cactaccagc aggaagaaac tcaccggggc gataagcggc agcgcaatct
    22621 taaagaagcg gcgaatcggc cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg
    22681 gaatggattg cagcgcggca tagaagaaca gaaagttgta gctgatttgc ttccacaccg
    22741 aggcaaacac caccagaaac atcgcctgac cgctattttg cgcgtggttc cagtcgtagc
    22801 cgaactcagc gagaaaatgg gtgatcagcc cgcgaccggg gttaaacagg aagatccaca
    22861 atacggcggc aacggcggga gccacggcat acggcagcaa cattaaggtt tggtagaaac
    22921 gactgccgcg cacgatgtac tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc
    22981 tgacggtgac aaaggtgctg aattttatcg tcgtccagaa ggagtcgaga taatagctgt
    23041 catgaaacag cgtgacgaag ttatccagac cgacaaactg gctggagaaa ccaaacggat
    23101 cgacgctttg tagcgagtac cacaacgctt cgcccgcagg ccagataaaa aagataacgg
    23161 tgatgatgag ctgcggcgcg accagcagat aaggcagcca gcgcgagcgg aacaccggac
    23221 gggatgatga cattacggtt aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc
    23281 tacacgcgat gccaggtcag ataagcgaaa cgcatccggc attacactga ttaagacttc
    23341 gtcgatttct caaagcggcg cagtaactgg ttcccacgct caactgcggt atccagcgcc
    23401 tgctgtggcg tcttcttacc ggtccacacg ctctcaagct cttcatccac aatcacgcgg
    23461 atctgcggca tgttgcccag acgaagccct ttggtgaacg gcaacggcgg cttgttcagc
    23521 atctgacgcg tcgcggtatc cgcacctggg tttttctcgt aaaagccctg ctcacgggtc
    23581 aggtcatacg ccgctttggt aattggcaga taaccggttt tctgatgcca ctcagcagcg
    23641 ttttctggct tcgcgaggaa gtcgagaaac ttcgccacgc cggtatacgt ttctttatct
    23701 ttgccctgca tcacccacag gctggctccg ccgataatgg cgttttgtgg cgcatctttc
    23761 gcatcggcgt cgtaaggcat catacctacg ccgtagttaa atttggcgta ctcgcgaatg
    23821 ttggcaagag aaccggaaga ggcggtggtc atcgcgcaat caccgttata gaacttctcg
    23881 gtggattcat ccttacgacc gacgtagctg aagtcgccct tcttgttcat ctcctcgagc
    23941 atggcgatgt gtttcacctg ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca
    24001 aagccgttgt ttttgctggc aaacggcaga ccgttccagg cgctaaagtt ttccagttgg
    24061 atccagccct gccagccgct ggcgtagccg cacttaatac cggaggcttt cagttttgcg
    24121 gaatagtccg ccagatcctg ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc
    24181 ttgaaggcgt ctttgttgta atagagaacg gaagtcgagc tgttgaacgg ctgggagagt
    24241 aagtggccgg ttttgctgtc ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca
    24301 tcgaactgaa tccctgcctc tttaaacacg tcatacaccg gtttaatggc tttcgacgcc
    24361 atcatggtgg cggtgccaac ttcataaacc tgcaaaatag ccggagcgtt gccggtacga
    24421 aatacggcaa tccccgcgct taaattctgt tcgtagttgc ctttataggt cggtacaatt
    24481 ttgtaatccg gattttcggc gttaaaacgt tgggccagag aatccacctc tttacccagt
    24541 tccccttcca tagaatgcca gaacggaatg gtcgtcactg cctgtgcatt ccccattaac
    24601 gccagtccga gcgccagtgc tgaagctgta taatgtaacg gtttcatcgt ttatctctct
    24661 tgttgtaccg aatgcgcgaa ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg
    24721 aattacagaa aaataacttt tttgttacat ttgtaagata gtaaggtgtc agaaagatga
    24781 caaggcggtg acggcgtggg tgagggaaaa tgggagatgg ggcacggata agcggaaaaa
    24841 tagaaggtct gaatcaaact ctacagatta ctcatcgttt catgccggat gcggcgtaaa
    24901 cgccttatcc ggcctacaag atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc
    24961 tgataagcgc agcgcatcag gcaattttgc atttgtcacc tgtctcaaag gagtcttttg
    25021 actccttatc aatcaacgtg ttattacccg cctaaatacg cacttctcac tgcttcgttc
    25081 gccagcagcg cgtcgccggt atcggagagc accacatggc cgttttccag cacgtagccg
    25141 cgatccgcca gctttagcgc ctggttggcg ttctgctcga cgaggaagat agtcatcccc
    25201 tgctcgcgca gctgctcgat ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg
    25261 agcgatggct catcaagtaa caacaaacgc ggattgctca tcagcgcgcg accaatcgcc
    25321 agcatctgct gttcaccgcc ggacatggtg cccgcccgct gaacacggcg ctcatgcaga
    25381 cgtggaaaca gttcatacac ccactttatg cgctcctgaa actggtcacg ttcggcaaag
    25441 aagccgccca tcgccaggtt ctcttccacc gtcatccgcg agaagacgcg acgcccttcc
    25501 gggacaatcg ctaccgcttc gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta
    25561 tcatcaaaca caattcgccc gctggtggcg cgcggatcgc cgcataacgt gccgagcaag
    25621 gtggttttcc ccgccccgtt cgcgccaatg agagtgacaa tctcgccctg attgatatgc
    25681 aggctcacct catgcagcgc ctggattttg ccgtagtggg cgctgacttt gtcaaaggac
    25741 aacatgactt tttccatctt atgcctcacc taaataggca cggatcacgt ccgggttatt
    25801 acggatttgc tccggcgtac cgtttgccag tggcgtcccc tgattgacca cataaattcg
    25861 gtctgaaatc cccatcacca gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg
    25921 atgattgcgc agttcggcaa tcagctcatc cagctctttg gtttcttttg ggttaagacc
    25981 tgccgccggt tcgtcgagca ttaaaatctc cggctgcgtc accatgcagc gggcaatctc
    26041 cagacggcgt tggtcaccat aggccaggtt actcgcctga cggttggcgt gttccagcaa
    26101 accaatgcgc tcaagccagg tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa
    26161 agaaggcgtt ttcaacaggc cagagaacag cccggttttc agttgctgat gctgcgccac
    26221 cagcaggttt tcaattaccg tcatttcacg gaacagacgc acatgctgga aggtgcgcac
    26281 cacgcccatg cgagcaattt gctgccccgg taaaccttca agatgctgat cgcgcagtaa
    26341 aatggtgccg ccggtgggtt tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc
    26401 ggcaccgtta gggccgatta acgagacgat ttcctgcggg tacagttcaa gattgacgtt
    26461 gttcaccgcc agcaggccgc cgaagcgcat catcagaccg ttaacagata ataatggctg
    26521 actcatgcct gctctccttt cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg
    26581 ggcagcaaac cctgcggacg ccagatcatc atcagcacca tcaaaccacc gagcattaac
    26641 atgctgtatt cgttgaaatc acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc
    26701 agaatcactg caaattgcga gcccataccg ccgagcacca ctatcgccag cacaaaggcc
    26761 gattcagcaa aggtgaagga ttccgggctg acaaagccct gacgcgccgc aaacagcgtt
    26821 ccggcaaaac cggcaaatgc ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg
    26881 cttaagccca gcgaacgaca ggcgatctcg tcttcacgca acgcttccca cgcgcggccc
    26941 agcggcatcc gcagcaggcg gttaatgaca aacaggctta acaccaccag cagcaacgcc
    27001 accaggtaga ggaagataac gcgatcggac ggatcgtatt tcaggccaaa gaaattactg
    27061 aacgtgtccc agccgccttc acgagcggta cggctgaact cgagtccgaa gagtgtcggt
    27121 ttcgggatct gactgattcc gttcgggccg ccggtgattt cagtgttatt gagcagcaat
    27181 atgcgcacaa tttcgccgaa accaagggta acgatcgcca gatagtcacc gcgcagacgc
    27241 agcaccggaa aaccgagcag gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa
    27301 caggtccaga accccagacc gtaatagtga ttgagcagcg caaaagtgta agcgccgatg
    27361 gcgtaaaaac cgccgtaccc cagcaccagc agaccagaaa gaccaacaac cacattcagc
    27421 ccgagaccga ggataatgta gatcatggtc agagtggcga tatccaccgt cccacgtgac
    27481 accataaacg gccacgccac cacaagcacc agcagcgcca cgaggaacag cttttgcttc
    27541 accgtggacc catcaatcgc gggcagaata aacttcggcc cggaaacgct tttcaaccct
    27601 ttctggaaag ccggtcgcaa aagctggaag aaaaagacca ccgccgtgcc gataaacatc
    27661 cactgccaac ggatgtctga agccgtgtcg accaccagtt tggtgccatc cagctccagt
    27721 tgtacgccca taaagacgcc cgccaggaca aagaacatcg cggcagagag cagtgccatt
    27781 gcaatatgca tcggtttcat actttctcta cctccgggcg acccagaata ccggtcggca
    27841 tcaccagcag caccagaatc agcagggcga atgagaccac atctttatat tccgtactca
    27901 gataggcaga agagagcgcc tccgcaatcc ccaggatcag cccgccaatc atcgcccctg
    27961 gaatgctgcc aatcccaccg agcaccgcag cggtaaaggc tttcatcccg gccataaagc
    28021 cgatataggg gttaatgacg cagtagaact gaccgagcag tacgccagcc actgccgcca
    28081 tcgccgcgcc aataacaaag gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc
    28141 tcgccatttt cagatcttcc gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa
    28201 tgaaaatcgt cagcgccagc atggcgagga aggtgacaat ccagatcacc gcctgcatgg
    28261 tggtaataga ggcagagaag ttttcgctat ggcccaccac ccactgaccg ttaaacaggc
    28321 tcggcagcgc cacgtcgcgc gaaccttcgg tcaggctgac gtagttttgc aggaagatgg
    28381 acataccgat tgcggaaata agcgcaatca ggcgcttaga gttacgcacc gggcggtaag
    28441 ctacccgttc gatactccag ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg
    28501 ccgccaccag cagccagccg gtatcaatgc ccatcatcat cagcgcggcg atgatcataa
    28561 atgagacgta gctgccaatc atataaacct cgccgtgggc gaagttgatc atgccgataa
    28621 tgccgtaaac catggtgtag ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc
    28681 cgttaaacat ctgctgcaag aaatacaaaa actgctcaga cataaggtaa cctttctaaa
    28741 cccgcccgca ttttacgggc ggtgggatga tcacttggct gccgtggatg aaccgtcggc
    28801 gtgccactgg aagacaccaa aatcaaatcc cttaagatcg cctttttcat cccagttcag
    28861 cggcccaatc acggtgtttg caccgttagc ttttaaatct ttcaccagcg ccagcggctc
    28921 atcgctgccg gtacgctcaa gggcagttgc cagagattgc actgccgcgt aagtgatcca
    28981 gacataaggc ccggacggat ctttcttgtc tgctttcagc gcatcaacga tgccctggtt
    29041 tgccggatcc tggtcatagc gttttggcat agtgaccaac atgccttcgg cggcatcacc
    29101 ggcaatgttc gacagcgatg cgttaccgac accttccggc cccataaact gggttttcag
    29161 gccaacggaa cgggcttggc gcagcatctg ccccatttcc gggtagtaac cgccgtagta
    29221 aacgaagtcg atattttctt ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc
    29281 ggcggtaata ccgtcgaaga agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac
    29341 cgaacgcgcc agcccttcgc catactgttg tttgtcgtga atgatggcga tgcgctgggg
    29401 cttcaccgtc tcaagaatgt attttgccgc cgtaggcccc tgggaagagt ccagcccggc
    29461 agtacgcata atgtgttgat aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga
    29521 gatcatcaga ataccttcgt cttcgtagat atccgacgct ggctgggtag aggaagaaca
    29581 cagatgacca ataacgtatt taatgccgtc gttaacgatt ttgttggcga ccgcaacggc
    29641 ttgtttcggg tcgcaggcgt cgtcatattc cacgccaacc agcttgtcgc ccttaattcc
    29701 ccctttgaca ttaatgtctt taatcgcctg acgcgcgccg ttaaattcca tatcgcccca
    29761 ctgggcaatc gggccggaca tcgcgccgac aacggcgact ttaatatcgt cagccatagc
    29821 ggtgtgtgaa attgccagtg caatcatccc tgcgatgata gttttcgcat tccgtttcat
    29881 agtcaaaaat ccccattcgt gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga
    29941 ctgttctttt tttatactgc actgtttttg cctgtctgat taaggggtta gcgcagtatt
    30001 ttgtgatgat agcgattaaa atccctattt ttcagtcgat taagaacaga taatattctg
    30061 aatttattga tagataaaca gaaaaaagtg cctttgtcag cataaaataa cggcacaaag
    30121 ggcggaataa ttcactatca ttcaggggat tatgctggac atttttcatt ctctaatgtt
    30181 ttaactttgt aattattgct gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag
    30241 aaagcctccg attaaattat ttcgctacac tggttccact tttgtgattt acacgggtta
    30301 cccatgaagc tgaccatcat tcgattagaa aactttagcg accaggaccg gattgacctg
    30361 caaaagatct ggccggagta ttccccttcc tctttacagg ttgacgataa ccaccgtatc
    30421 tacgccgcgc gttttaacga gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc
    30481 gagggagcac tggattctct acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa
    30541 tatctgctgg aagaggtttt gcgtaacaat cctggcgttt catgctggtg gatggcggat
    30601 gcaggcgtgg aagatcgcgg tgtgatgacg gcgtttatgc aggcactggg atttacggca
    30661 cagcagggcg gctgggagaa gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg
    30721 agaaattgcc tgatgcgcta cgcttatcag gcctataatg tgtgctgcaa tttactgatt
    30781 tctttggttc ttgtaggccg gataaggcgt ttacgccgca tccggcatga agcaacgtat
    30841 tcgatattag caatttggcg gcaacccaaa gttgccgatt aacaattact tcgcatcggt
    30901 cgccgtgccg ttggcgtgcc agtcaaatac gccgaactca aagcccttta gatcgccttt
    30961 ctcatcccag gtcagtggcc ccatcacggt atccacggag ttcgctttta ggtatttggc
    31021 gatttcagcc ggatcgtcag actgattcag gcccgcctgc aaagattgca gcgcggcgta
    31081 ggtggtccag acgaatgcgc cgcttgggtc ctgttttttc gctttgatcg catcaacaat
    31141 gggtttgttc gccggaacct gatcgtagtt cttcggcttg gtcaccagca gcccttccgc
    31201 tgattcgccc gcaatgttag acagcgaaac gttagccaca ccttccggcc ccataaactg
    31261 agttttcagc cctgccgcgc gtgcctgacg caggatttgc cccatttccg ggtgataacc
    31321 gccgtagtaa acgaagtcga tattctcttt tttcagacgc gccaccagcg ttgagaaatc
    31381 tttttccccg gcggtgatac catcaaagaa caccacgtta gcattgcctt tcttcaggcc
    31441 gtcctgcacc gctcgtgcca gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat
    31501 acgctgcggt ttcactttct caagaatata tttcgccgcc gtcggcccct ggtcggagtc
    31561 caggccggtg gtgcgcagga tcagctgata gccacgggcg gtcagctccg gcgcggttgc
    31621 cgctggggtg atcattaaaa tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga
    31681 tgaagaacag aggtgaccaa tcacatattt aatgccgtcg ttaacgactt tgttcgccac
    31741 cgcaaccgcc tgtttcgggt cacaggcatc gtcatatttt acgatttgca gtttgttgcc
    31801 tttaatgccg cctttagcgt tgatatccgc aaccgcctgc tctgcgccgg taaactcctg
    31861 gtcaccgtac tgcgcaaccg gaccggacat tgcacccacg accgcgactt taatatcttc
    31921 tgccagagcc atattgctga atgccagcgc gatacatcct gccagtaacg ctttaccctt
    31981 tatgttcatc ctgagaatcc ccattcttct ggttattacg tgtgttgtga tgttgttgtt
    32041 cagcacttta tttcgtttta tgcatgacta cccgtgcttt agcagcatac tctgctaaaa
    32101 catacccgat ttttatgata ttggaatagc tattttgaca gtttattaac aatctgcgtg
    32161 gggattggcg ttttgccgga gggggaattg attattgtag aggcccaaaa aacaaaaacc
    32221 ccggactctc atccagggtt ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg
    32281 aagttttttc atcgcgttct tttccagttg gcgcacacgt tcagcggaaa caccgtaacg
    32341 gtcagccagt tcctgcaacg tggacttgtt gtcttcgtcc agccagcgcg cacggatgat
    32401 gtcctggctg cgttcgtcca gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc
    32461 ttcccagtta tcatcttcga tgccgtcggc aaagttagat gatttatcct gcagatagag
    32521 caccggtgcc atcggctggc tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc
    32581 ctgtgccgcc atacgtgatt ccatctcacg tacgtctttg ctggttacgc ccagttcacg
    32641 ggccaccatt tcgacttcat cctggttaaa ccagcccaga cgctgcttgg ttttacgcag
    32701 gttgaagaac agtttgcgct gcgctttggt ggtcgcaact ttgacgatac gccagttacg
    32761 cagaacgtat tcgtggatct ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac
    32821 acccacttcc gggttaaaac ggcgcactgc tttcatcagg ccgatgttac cttcctgaat
    32881 caaatccgcc tgtggcaggc catagcccgc ataattacga gcaatatgaa caacaaaccg
    32941 caggtgagac aggatcagcg ttttagctgc ttccagatcg ccatggtaat gcagcttttc
    33001 agccagcgcc cgctcctcgt cagccgacaa catcggccac gcgttagctg cccggatgta
    33061 ggaatccagg ttgccaactg gggctaaagc taaactttgc attttgtcag tcattcaaat
    33121 cctctcaatc gatatcttct ggcgcttcag tggtagcaac aactgtgcca aagcttaaga
    33181 gcaacgaggt tatcattcac tgttttatca gaccgtgatt ttatccacaa gttcaatgca
    33241 agcttgtgaa taaattacgc acaaaatgtg acatagagat gaaataccgg gaagagacaa
    33301 cggggtctct ttccctgcta cggaacccat tgcagggaaa gagtataaca cgcttttatt
    33361 attcaggcgt aaagtggcgt aaatgttgta ccgtggcaag ccacgctgcc acccagccaa
    33421 tcatcgagca taccagcagc aatagcaggc attcatcgaa cgataaacca ttgatatcaa
    33481 acttcgttcc gaaaacctgt gccacttccg ccaccgctga cgacaaacgc agtaccagaa
    33541 tttctgacaa aattaatgac aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca
    33601 ggaacgggcg aaggatgaaa ccatccgtcg caccaatcag tttctgcacg ttgatggagt
    33661 cacggcgagc aaagatgctc agacgtacgc tgttgccgat gacgaggaac actgccgcca
    33721 ccatcaacac gccgatcatc gccgaaacgc gcccaaccag cccggtcaac gccgccagac
    33781 gggcaaacca gctgtcatcc atccgcactt cgtcaatacc gttaatctgc gtgatacgat
    33841 cacgcagcgt attgagtgat tccgtcccct gaaaatcgag tttcgggatc accaccgcca
    33901 ctgccggaag cgggttttct tccagcatat ccagcgcacc accaaaacca gaccagttac
    33961 ggaactcgcc cagtgcgtct tcacgagaaa gatagttcac tttctccacg ccttgctcgg
    34021 cctgcaactg tgccaccacg cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa
    34081 cagtgatttg cggtgacgga taatactgcg tcgccgcctg attaacgttt ttgtacacca
    34141 tataacagac gctgggcagc gtcagggaga tggcgataac catcaccgtt aaaaacgtgg
    34201 cgaacggttt gcttttcaga tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt
    34261 tgaaaacgtt ggttttgcga tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt
    34321 tacgaccgcc gtcgcctgag ccgccgaccg atttacggaa gcgatccaga cgcccaccaa
    34381 actgccgaat atgattgatt gcatcgcgct tattcatggc ccacgcctcc atgcaagtga
    34441 ccatcgctca gggtgagcat gcgataggaa cgccgtgaaa tcagattaat gtcgtgcgtt
    34501 gccatcaata cggttacccc aacgcggtta aactcttcaa acagacgtag aatcccttcc
    34561 gacaacgcgt cgtccaggtt accggtcggt tcgtccgcca gcagtaccgc gggcttattc
    34621 accaccgcgc gggcaatgcc aacacgctgt tgttcaccgc cggaaagctg aatagggaag
    34681 ttcttcgctt tgtccagtag cccgacttta tccagtgccg ccgacacccg gcgacgaata
    34741 tcgtcaccgc tggcaccagc gataatcagc gggatcgcca cgttatcgta gacagtacgg
    34801 tccatcagta gatggtgatc ctggaaaatc atgccaatct ggcggcgcag aaacggaact
    34861 tcacggtttt tcagacgcgt gatgtcatgg ccgctaaacc agattttccc ggcgctgggc
    34921 cgctcaatcc cacagatcag cttcaggagg gtacttttcc ctgcgccgga atgaccggtc
    34981 agaaacgcca tctcacccgg ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca
    35041 ccgagataag ccttgctgac atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa
    35101 gtgcctctat aaagtcgtcc gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac
    35161 caatgtagcg gatagggata ccaaactggt cagccaccga gaaaattacc ccgcctttcg
    35221 ccgtgccgtc cagtttcgtt agcgtgatgc cggttaagcc aacagcttcg tggaacagtt
    35281 tggcctggct taccgcgttc tgcccggtgc tggcatcaat agtcagcata acttcatgcg
    35341 gcgcttcaac gtcgagtttc ttcatcacgc ggacgatttt cttcaactct tccatcagat
    35401 gcgatttgtt ctgcagacgt ccggcggtat cggcaatcag gacgtcgata ttacgcgctt
    35461 tagctgcctg aatggcgtcg aagataacag aggcggaatc cgcaccggta tgctgggcaa
    35521 tcaccggaat attgttgcgc tgaccccaga cctgaagctg ttcaaccgca gctgcacgga
    35581 aagtatcgcc cgccgccagc atcaccgatt taccctgctg ctcaaactga cgcgccagct
    35641 taccaatcgt cgtggtttta cccacaccgt tgacgcccac catcaggatc acgaacggcg
    35701 ttttatcttc aacattcagc ggctcatcga ctttcgccag aatctcgccc atctcttctt
    35761 tcagcaggcc atagagcgcc tcggcatcac gaagctgctt gcgggatgcg ccttccgtca
    35821 gattggtgat aattttacgt gtggtttcca cgcccacatc ggcgatcaac agctgttctt
    35881 ccagctcttc aaacagatca tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg
    35941 aaccgagatt ttctttggtt tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt
    36001 tggtcggttt ttcttgctcc tgggcaattt ctgcaaccgg ctgctcttct tctgccggag
    36061 gaactaccat caccgcctct tctgccgctt cggcagccag cgcctgtgct tccagctctt
    36121 cgtcggtgat ttcttcttta gccgcttctt cttcttctgc cgcttcgaca atctctacgg
    36181 tttccgcttc agcctgccac tcttctgacg aaacctcttc ggcgttgacg tcttccggca
    36241 acggcagctc ttcacgttcg atagccactg gctccggcgt ttcttctacg accggttccg
    36301 gctgtgcaac aacttccgct tcaggctgcg ctttttcact ttcaacaacc tgttcagtga
    36361 cttccacaac gtcggcagca aaagtttccg cctcggcttc agtatgcgcc tgcggctgct
    36421 cttcaacggc ttgttcagaa gccttcgcag gctcttgcgc ctgaacgatt tcttctacaa
    36481 ccggttgttc attctgaact tctgtctctt tttccggggt ctgctctttt tgaccaaagc
    36541 ccagccagga aaaaaagcca cgtctttttt ctttcgccat ttgcgactac actcctcgct
    36601 gttgcttcat ggcacagcgt taacgctatg tacatagcag ctaaaaaaat gatgaaatag
    36661 tctatcactt aacttaattc acatcaccgc ctgcaagtat gtgttatctg gcggattgag
    36721 caatttatca tgaaaaaacc gaatcattcc ggcagcggcc aaatccgcat tattggcggg
    36781 cagtggcgag gccgtaaact cccggttcct gatagcccag gtctgcgccc caccaccgac
    36841 cgcgtacgcg aaacgttgtt taactggctg gctccggtca ttgttgacgc ccaatgtctg
    36901 gattgcttcg ccgggagcgg cgcgctgggg ctggaagcgt tatcgcgcta cgctgcgggg
    36961 gcaacgttga ttgagatgga tcgcgtggtt tctcagcagt taattaagaa tctggcgaca
    37021 ctaaaagcag gcaatgcacg cgtggtgaac agcaacgcaa tgtcattcct ggcgcaaaaa
    37081 ggcacaccgc ataatatcgt gtttgtcgat ccaccgttcc gccgtggctt gttagaagag
    37141 acgataaatt tactggaaga taacggctgg ctggctgacg aagccctgat ttatgtcgaa
    37201 agcgaagtcg aaaacggtct gcccactgtt ccggcaaact ggtcattaca tcgggaaaaa
    37261 gtggcgggtc aggtggctta tcggctgtat caacacgaag cacaaggaga aagtgatgct
    37321 gattaatatt ggtcgtttgt taatgctctg cgtttgggga tttttaatcc tcaatctggt
    37381 gcatcccttc ccgcgcccgc taaatatttt cgttaacgtg gcgctgattt ttaccgtgct
    37441 gatgcatggt atgcagctgg cgctattgaa atccacttta ccgaaagatg gcccgcagat
    37501 gaccaccgcc gaaaaggtgc ggattttcct tttcggcgtg tttgaactgc tggtctggca
    37561 gaagaaattt aaagttaaaa aataacctta ttgttcgcca acaaagctaa caaagcgcgt
    37621 tcctttatag ctcagcgtac ctttatcgcc cactgtcagg gcgtggtact gctgggcgtc
    37681 gaggcgaaac gtctgctcca ttcctccgct ttgcggtttg aagcttgcct catagcgcat
    37741 actggtgcct gccggagtca cttcctgctg gcgcgaacgg cgatcgttaa tcggtttttc
    37801 ccgcttgttg ctcaccacca ccagcttttg ctggagcgga gccatatcat tatcagcttt
    37861 ttcccgtcgc tgttgcataa aacgaaacga tgcggcgacg acaattaagc caatgataac
    37921 aataaagaaa agaggtggtt tactcatctt tatccctcat cggaaaatgc ggaattaagc
    37981 ataccctgcc agttatggtg ttgtcatccg tccaccctcg ccactaaact ggaagcaaga
    38041 ccgtaggcat tctgcttacg aaaaaataac gaattcaagg aactaagatg ctttggtcgt
    38101 ttatcgctgt ctgtctttcc gcatggctat ctgtggatgc atcgtatcgt gggccaacct
    38161 ggcaacgctg ggtgtttaaa ccgttaaccc ttcttctcct gctgttactg gcctggcaag
    38221 caccgatgtt cgacgccatt agctatctgg tgctggcggg actgtgcgcc tcactgctgg
    38281 gcgatgcgct aaccctgttg ccacgtcaac gtctgatgta cgccatcggc gcgtttttcc
    38341 tctcgcacct gctgtacacc atctatttcg ccagtcagat gacgctctct ttcttctggc
    38401 ctctgccact ggtgttgctg gttctgggtg cgctgctact ggcgattatc tggacgcggc
    38461 tggaagagta ccgttggcct atctgcacgt ttatcggcat gacgctggtg atggtgtggc
    38521 tggcaggtga actgtggttc ttccgtccga ccgctccggc gctctctgcg tttgttggcg
    38581 cttcgttgct gtttatcagt aactttgtct ggctggggag ccactatcgc cgacgcttcc
    38641 gtgcggataa cgcgattgct gcggcctgct actttgccgg tcacttcctc atcgtccgct
    38701 cactgtatct ctgataaaac ttgactctgg agtcgactcc agagtgtatc cttcggttaa
    38761 tgagaaaaaa cttaaccgga ggatgccatg tcgacacctg acaatcacgg caagaaagcc
    38821 cctcaatttg ctgcgttcaa accgctaacc acggtacaga acgccaacga ctgttgctgc
    38881 gacggcgcat gctccagctc gccaacgctc tctgaaaacg tctccggcac ccgctatagc
    38941 tggaaagtca gcggcatgga ctgcgccgcc tgtgcgcgca aggtagaaaa tgccgtgcgc
    39001 cagcttgcag gcgtgaatca ggtgcaggtg ttgttcgcca ccgaaaaact ggtggttgat
    39061 gccgacaatg acatccgtgc ccaggttgaa tctgcggtgc aaaaagcggg ctattccctg
    39121 cgcgatgagc aggccgccga cgagccacaa gcatcgcgcc tgaaagagaa tctgccgctg
    39181 attacgctta tcgtgatgat ggcaatcagc tggggtctgg agcagttcaa tcatccgttc
    39241 gggcaactgg cgtttatcgc gaccacgcta gttgggctgt acccgattgc tcgtcaggca
    39301 ttacggctga tcaaatccgg cagctacttc gccattgaaa ccttaatgag cgtagccgct
    39361 attggtgcac tgtttattgg cgcaacggct gaagctgcga tggtgttgct gctgtttttg
    39421 attggtgaac gactggaagg ctgggccgcc agccgcgcgc gtcagggcgt tagcgcgtta
    39481 atggcgctga aaccagaaac cgccacgcgc ctgcgtaacg gtgagcggga agaggtggcg
    39541 attaacagcc tgcggcctgg cgatgtgatt gaagtcgccg caggtgggcg tttgcctgcc
    39601 gacggtaaac tgctctcacc gtttgccagt tttgatgaaa gcgccctaac cggagaatcc
    39661 attccggtgg agcgcgcgac gggcgataaa gttcctgcag gagccaccag cgtagaccgt
    39721 ctggtaacgc tggaagtgct gtcagaaccg ggtgccagcg ccattgaccg gattctgaaa
    39781 ctgatcgaag aagccgaaga gcgccgcgcc cccattgagc ggtttatcga ccgtttcagc
    39841 cgtatttata cgccagcgat tatggccgtc gccctgctgg taacgctagt gccgccgctg
    39901 ctgtttgccg ccagctggca ggagtggatt tataaaggtc tgacgctgct gctgattggt
    39961 tgcccgtgtg cgttagttat ctcaacgcct gcggcgataa cctccgggct ggcagcagca
    40021 gcgcgtcgtg gggcgttaat taaaggcggt gcggcgctgg aacaactagg tcgtgttacc
    40081 caagtggcgt ttgataaaac cggtacgctg accgtcggta aaccgcgcgt taccgcgatt
    40141 catccggcaa cgggtattag tgaatctgaa ctgctgacac tggcggcggc ggtcgagcaa
    40201 ggcgcgacgc atccactggc gcaagccatc gtacgcgaag cacaggttgc tgaactcgcc
    40261 attcccaccg ccgaatcaca gcgggcactg gtcgggtctg gcattgaagc gcaggttaat
    40321 ggtgagcgcg tgttgatatg cgctgccggg aaacatcccg ctgatgcatt tgctggtttg
    40381 attaatgaac tggaaagcgc cgggcaaacg gttgtgctgg tagtacgtaa tgatgacgtg
    40441 ctgggtatca ttgcattgca ggataccctg cgcgccgacg ctacaactgc catcagtgaa
    40501 ctgaacgcgc tgggggttaa aggagtgatc ctcaccggcg ataatccacg cgcagcggcg
    40561 gcaattgccg gggagctggg gctggagttt aaagcgggcc tattgccgga agataaagtc
    40621 aaagcggtga ccaagctgaa tcaacatgcg cccctggcga tggtcggtga tggtattaac
    40681 gacgcgccag cgatgaaagc tgccgccatc gggattgcta tgggtagcgg cacagacgtg
    40741 gcgctggaaa ccgccgacgc agcattaacc cataaccacc tgcgcggcct ggtgcaaatg
    40801 attgaactgg cacgcgccac tcacgccaat atccgccaga acatcaccat tgcgctggga
    40861 ctgaaaggga tcttcctcgt caccacgctt ttagggatga ccgggttgtg gctggcagtg
    40921 ctggcagata cgggtgcgac ggtgctggtg acggcgaatg cgttaaggtt gttgcgcagg
    40981 agataaggca aaccgatcgc aacattgagc gcgatcggtc ccctcgcccc tctggggaga
    41041 gggttagggg gtgaggaaaa ggcggcatcg aagccaatca gcccctatca accgccttta
    41101 cgaatcaaat aacgataagg cagtccatcc gtctctttcg caaccagttc gtgttccata
    41161 aaggtacaaa acccaggaat atcgcgggta gtggccggat cgtcggcgat aatcagcaac
    41221 gtttcgccag gctgcatatt gcgcacggtt ttgcgcacca tcatcaccgg ttccgggcag
    41281 cgcaggccaa gcgcgtcgag tgtgtggtca gggctggaaa agagatcggt cattttcttc
    41341 tcatcactta aaaaaacggc gctagtttac gccctgtgag tccgtaagca aaccaggtta
    41401 acgattgcgt gaaaattaac cattgcattg tcaacgtaaa gcagtatcat gcggcggctc
    41461 gaaaaaaggg taagcacgtt attatgttaa ggtaacagac gtgtcgtacg tattgggttc
    41521 cctcacccca atggttaatc aaaaaggtac aatatgaacg ttttctcgca aactcaacgc
    41581 tataaggcgt tgttctggtt atcgttattt catcttctgg tgatcacctc cagtaactat
    41641 ctggttcagc ttcccgtctc catttttggt ttccatacca cctggggcgc gtttagcttt
    41701 ccgtttattt ttcttgctac cgacctgacc gtgcgtattt ttggcgcacc gctggcccga
    41761 cgcattatct tcgcggtaat gatccctgcg ttattaatct cctacgtcat ctcgtcgcta
    41821 ttctatatgg gttcctggca gggattcggc gcactcgccc acttcaacct gtttgtcgcc
    41881 cgtatcgcca ccgccagttt catggcctac gcgctggggc aaatcctcga cgtgcacgtt
    41941 tttaaccgcc tgcgtcagag tcgccgctgg tggctggcac cgacagcgtc cacactgttc
    42001 ggtaacgtca gcgacacgct ggcctttttc ttcattgcct tctggcgtag cccggatgcc
    42061 tttatggctg aacactggat ggaaatcgcg ctggtcgatt actgtttcaa agtgttaatc
    42121 agtatcgttt tcttcctgcc aatgtatggc gtattactca atatgctgtt gaaaagactg
    42181 gcagataaat ccgaaatcaa cgctttgcag gcgagttaaa ggttcgttat cagagttgtg
    42241 ataagatgga tgaatgagcc gttatggccg tttatcgaaa ggaagaagtc aatgcgcaat
    42301 ctggttaaat atgtcggaat tggcctgctg gttatggggc ttgcggcctg tgatgataaa
    42361 gacactaacg ctacggcgca gggttcggtt gcggaaagta acgctaccgg gaatcccgtc
    42421 aacctgcttg atggcaagtt aagtttctcg ctgccagcgg atatgaccga ccagagcggt
    42481 aagctgggaa cgcaggccaa taacatgcat gtctggtccg acgccaccgg gcagaaagca
    42541 gtcatcgtca tcatgggcga tgatccgaaa gaagatctgg cggtgttggc gaagcgtctg
    42601 gaagatcagc aacgtagccg cgatccgcag ctgcaagtgg taaccaataa agccattgag
    42661 ctgaaaggtc acaaaatgca gcagttagac agtattatct ccgcgaaagg ccagacggcg
    42721 tactcttccg ttattctggg taacgtgggt aatcaactgc tgaccatgca aattacgctg
    42781 ccagctgacg atcagcaaaa agcgcaaacc accgcagaaa acatcattaa tacgctggtt
    42841 attcagtaag ttttaagatg atgaggcggc ctcagggacg tgttccggag gccgtttttt
    42901 taatcgccac gtcagtaata acgcgattgc gaccagtccc gccgccgcca gataaatcac
    42961 cggtacgccc gcccagctca tcaccagccc agccagtggt ccagtcacgc caagcgataa
    43021 atccataaat acggtgtaag ttgccagcgc cgccccctga ttttgctgcg gaaccgcttt
    43081 taccgccact acacccaatg ccgggaacac cagcgaaaac ccggcccccg ccagtaagac
    43141 gccgattttc gccatccacg gcatagtcgc cacgccaacc agtagcaggc cgattatctc
    43201 aacgctaaag caaatcatcg ctacgtttaa gccaccgata cggttaatgc cgttagggaa
    43261 taacaaacgc gtaccgacaa acgcacagct aaacagcgtc agcgcgaaag ccgcaccgtc
    43321 ccaaccttta gcgtcataaa acagcgtgat aaaggtggcg atgacgccaa atccggcgga
    43381 agccagtgcc agcgccatac cgtacagcca gacgcgccca agcaccgcgc gaaacggcag
    43441 cggtttgcct ttactggctt ttaccgtcgg acgcgggatc gccaacaaaa tggccaccag
    43501 cgccacgccc ataatgatta acgctaacgc ctgcaagccg ccccagtgat aaaacacgac
    43561 gcctaacggc gcacccatcg ccatcgcccc gtaagtgaca atgccgttcc acgaaatcac
    43621 ccgcccgata tgcagcgagc caaccactcc aacgccccat agggtcgatc ccgttccggc
    43681 aaaactttgc ccaatcccaa ggatgacgcg ccccaggcaa agtaataaca ggctgatgac
    43741 aggcagactg gcggttaatc ctgccgtcag ataccccaga ccgctcaaaa agcagccgca
    43801 taaaccgaag acgacaatct ttttgggtcc cagcaaatcg gcgtaacgtc cggcatgagg
    43861 gcggctcagc aaggtggcga aatattgcag gctgataacc aatcctgccc agaaggcgct
    43921 aaagcccatc acatcatgga catagcccgg taatacagcg agcggcaacc cgatggtgag
    43981 gtagctggcg aagttaaaca tgacaatgga gacaatgcgc aaattcaggc gcaatccgtt
    44041 tagcgcgggt tcggctacgg gttcgggcat gaggatcacc acatttttac aacagtgttt
    44101 catttttacc atgtgctgac gtgaaaatca gcagtaagaa tcagaatatt gctggcgtga
    44161 ctcccgcgct acacttaaca caaaaagtca caaggaagcc ccaatggaaa cccctcaacc
    44221 cgataaaacg ggcatgcaca ttctgctcaa gttggcctcg ctggtagtga tcctcgcggg
    44281 cattcacgca gcggcagata tcattgtgca gctgttactg gcgctgtttt ttgccatcgt
    44341 cctcaacccg ctcgttacct ggtttattcg tcggggagta caacgccccg ttgccattac
    44401 gattgtagtg gtggtgatgc tgatcgcact aaccgcgctg gtcggcgtac tggcggcatc
    44461 gtttaacgaa tttatctcta tgctgccgaa gtttaataag gagctgacgc gcaaactttt
    44521 taaattgcag gagatgttgc cttttcttaa tttgcatatg tcgccggagc gaatgctgca
    44581 gcggatggac tcggaaaaag tggttacctt caccacagcg ctaatgaccg ggctttccgg
    44641 ggcaatggcg agcgtgcttt tgctggtgat gaccgtagtt tttatgctgt ttgaagtgcg
    44701 ccacgtccct tacaaaatgc gttttgcgct gaataaccca cagattcaca tcgcaggatt
    44761 acaccgcgca cttaaaggcg tttcgcatta tctggcgtta aagacgctac tcagtttatg
    44821 gacaggtgtc atcgtctggc tggggctggc gctaatgggc gtacagtttg cgctgatgtg
    44881 ggcagtactg gcgtttttgc tcaactacgt gcccaatatc ggcgcggtaa tttccgccgt
    44941 accgccaatg attcaggtgc tgctgtttaa tggtgtttac gaatgtattc tggtcggcgc
    45001 attgttttta gtggtccata tggtcatcgg caatatttta gaaccacgga tgatgggcca
    45061 tcgcctgggg atgtccacca tggtggtatt tctttcattg ttaatctggg gatggctgct
    45121 cggcccggta gggatgctac tttcggtgcc attaaccagc gtgtgtaaaa tctggatgga
    45181 aacgacgaaa ggtggcagca aactggcgat tttactgggg ccgggcagac cgaaaagtcg
    45241 gttaccggga tgaggcgaca agtgatacga tacgcacttt cattttccat taaacgttgg
    45301 ccctgatatg tatcggatag ttctggggaa agtttcgacc ttaagcgcag ctccactgcc
    45361 accgggttta cgcgagcaag caccgcaagg tccacgacgc gaacgctggc tggcggggcg
    45421 tacattgctt tcgcacacgc tttccccgct accggagatc atctatggcg aacaaggcaa
    45481 acctgcattc gcgccggaaa cgccgctatg gttcaactta agccatagcg gtgacgatat
    45541 cgctctgctg ttgagcgatg aaggcgaagt cggctgcgat atcgaagtga ttcgcccgcg
    45601 cgtcaactgg cgctggctgg cgaacgccgt gttcagcctc ggggaacacg ctgaaatgga
    45661 cgccgtgcat cctgatcagc aactggaaat gttctggcgc atctggacgc gcaaagaagc
    45721 catcgttaaa cagcgtggcg gcagcgcctg gcaaatcgtc agcgtagata gcacctatca
    45781 ctcctcgctg tcagtcagcc attgtcagct agaaaattta agccttgcga tctgcacccc
    45841 cacccccttt acgctcaccg ccgacagtgt gcaatggatc gattcagtta actgatccgc
    45901 ccacccgact gcccatctat tgatccagaa caggtaatca gtatgacgaa tacttaaaat
    45961 cgtcatactt atttccgcca tctattttaa tccattgggg ttaccatgct ctccacactc
    46021 cgccgcactc tatttgcgct gctggcttgt gcgtctttta tcgtccatgc cgctgcacca
    46081 gatgaaatca ccaccgcctg gccggtgaat gtcgggccac taaacccgca cctttacacg
    46141 cctaaccaga tgttcgccca gagcatggtt tatgaaccat tggtgaaata tcaggcagac
    46201 ggttcggtga tcccgtggct ggcaaaaagc tggactcatt cagaagatgg taaaacctgg
    46261 accttcaccc tgcgtgatga cgtgaaattc tccaacggtg aaccgttcga tgccgaggcg
    46321 gcggcagaaa acttccgcgc agtgctcgat aaccgtcaac gtcacgcctg gctggagctg
    46381 gcaaaccaga ttgttgatgt taaagcactc aataaaacag agctgcaaat taccctgaaa
    46441 agcgcctact atcctttcct gcaagaactg gccctgcccc gtcctttccg ttttatcgct
    46501 ccctcgcagt ttaaaaacca tgaaaccatg aacggaatta aagcgccgat tggcaccgga
    46561 ccgtggattt tgcaggaatc gaaactaaat cagtacgatg tcttcgtccg taacgaaaac
    46621 tactggggcg aaaagccagc gattaaaaag atcaccttta acgtcatccc ggacccgact
    46681 acccgcgcgg tggcgtttga aactggcgat atcgacctgc tgtacggaaa cgaagggtta
    46741 ttaccgctcg ataccttcgc ccgctttagc cagaatccgg cttaccacac ccaactgtca
    46801 cagccgatcg aaaccgtgat gctggcgctc aataccgcca aagcccccac caacgagctg
    46861 gcagtacgtg aagctcttaa ttacgcggta aacaaaaaat cgctgattga taacgcgtta
    46921 tatggcaccc agcaggtcgc cgacaccctg tttgcccctt ctgtgcctta cgctaatctc
    46981 ggcctgaaac cgcgccagta cgatccgcag aaagcgaaag agttgctgga aaaagccggt
    47041 tggacgctgc ctgcgggcaa agacatccgc gagaaaaatg gtcagccgct gcgcattgaa
    47101 ctgtcgttca tcggcaccga tgcgttaagc aaatcgatgg cggaaatcat tcaggctgat
    47161 atgcgccaga ttggcgcaga tgtctcgctg attggcgaag aagagagcag tatctatgct
    47221 cgtcagcgcg acggtcgttt tggcatgatt ttccaccgca cctggggcgc gccatacgat
    47281 ccacacgcct tcctgagttc aatgcgcgta ccgtcgcacg ctgacttcca ggcacagcaa
    47341 ggattagccg acaagccgct gattgataaa gagatcggcg aagtgctggc gacccatgac
    47401 gaaacgcaac gtcaggcgct gtatcgcgac attctgaccc gtctgcatga cgaggcggtt
    47461 tatctgccta tcagctacat ctcgatgatg gtggtatcaa aaccggagct gggtaatatc
    47521 ccctacgcgc cgatcgccac cgaaattccg ttcgaacaga ttaaaccggt gaaaccttaa
    47581 tgttgcgtta cgtattacgc cgctttctgc tgctgatccc gatggtgctc gccgcctcgg
    47641 tgatcatttt tctgatgctg cgcctcggta ccggcgaccc ggcgctcgat tatctgcgtt
    47701 tatctaacct gccgccgacg ccggagatgc tggcctctac ccgcaccatg ctcgggctgg
    47761 atcagccgct gtacgtccag tacggtacct ggttgtggaa ggcgctgcat cttgactttg
    47821 gtatctcatt cgccagccaa cgcccggtac tggacgatat gctgaacttc ctgcccgcca
    47881 cgctggaact tgcaggtgcg gcgctggtat taattctgct cacttccgta ccgctcggta
    47941 tctgggcggc gcgccaccgc gatcgtttgc cggatttcgc cgtgcgcttt atcgcgtttc
    48001 ttggcgtgtc gatgcccaac ttctggctgg catttttgct agtgatggcg ttttcggtgt
    48061 atctgcaatg gctacccgcg atgggctacg gcagctggca gcacatcatt ttacctgcgg
    48121 tttccatcgc ctttatgtcg ctggcgatta acgcgcgact gctgcgcgcc agtatgctgg
    48181 acgtcgccgg tcagcgtcac gtcacctggg cgcggctacg cggcctgaat gacaaacaga
    48241 ccgaacgccg ccatattctg cgcaatgcct cgctgccgat gatcaccgcc gtggggatgc
    48301 acattggcga attgattggc gggacgatga ttatcgaaaa catctttgcc tggcctggcg
    48361 tcgggcgcta tgcggtgtcg gcgattttta accgtgacta tccggtgatt cagtgcttta
    48421 cgctgatgat ggtggtggtt tttgtggtct gtaatttgat tgtcgatttg ctcaacgccg
    48481 cgctggaccc gcgcattcgt cgtcatgaag gagcgcacgc gtgaactttt tcctctcttc
    48541 ccgctggtcg gtacgcctgg cgctgatcat tatcgccctg ctggcgctga ttgcgctcac
    48601 cagccagtgg tggcagccgt acgatccaca ggcaattgat ttgccgtcgt gcctgctttc
    48661 gccggatgcg cagcactggc tgggcaccga tcacttaggt cgcgatattt tctcgcggct
    48721 gatggcagcg acccgcgtgt cgctcggttc ggtaatggcc tgcctgctgc tggtgctgac
    48781 attagggctg gttattggcg gcagcgccgg gttgattggc gggcacgttg atcaggccac
    48841 catgcgcgtc gccgatatgt ttatgacctt cccgacctcg attctgtcgt tctttatggt
    48901 tggcgtgctc ggcaccgggc tgaccaacgt aattatcgcc atcgccctgt cgcactgggc
    48961 gtggtatgca cgcatggtgc gcagcttggt gatttcacta cgccaacgcg agtttgtgct
    49021 ggcgtcacgg ctttccggtg cgggccatgt gcgggtgttt gtcgatcatc tggcaggcgc
    49081 ggtgatccct tcgctgctgg tgctggcaac gctggatatc ggccatatga tgctgcacgt
    49141 cgcggggatg tctttccttg gcctcggtgt gaccgcgccg accgccgaat ggggcgtgat
    49201 gattaacgac gcgcgccagt atatctggac ccagccgctg caaatgttct ggccggggct
    49261 ggcgctgttt atcagcgtga tggcctttaa cctggtgggt gacgcactgc gcgatcatct
    49321 ggatcctcat ctggtgacgg agcacgcaca ctaatgccgc aacagattga actacgtaat
    49381 atcacgctac aggccgcgca gccgctggta cacggtgtat cgttaaccct gcaacgcggg
    49441 cgcgtgctgg cgttagtcgg cggtagcggc agcgggaaat cattaacctg cgccgcgacg
    49501 ctgggcattt tgcccgctgg cgttcgccag acggcggggg aaattttagc cgatggcaaa
    49561 ccggtttcgc cttacgccct gcgcggcatc aaaattgcca ccatcatgca gaacccgcgc
    49621 agcgccttta atccactgca caccatgcac acccacgcgc gggaaacctg cctggcgtta
    49681 gggaaacccg ccgatgacgc tacgcttacc gctgccatag aagcggtggg gctggaaaac
    49741 gccgcgcgcg tgctgaagct gtacccgttc gagatgagcg gcggcatgtt gcagcgcatg
    49801 atgattgcga tggcggtgct gtgtgaatca ccgtttatca tcgccgatga accgaccacc
    49861 gacctcgacg tggtagcaca ggcgcgcatc ctcgatctgc tggaaagcat tatgcaaaaa
    49921 caagcgccgg gaatgctgct ggtcacccat gatatgggcg ttgtggcgcg tctggcggat
    49981 gacgtggcgg tgatgtcaca aggtaaaatt gtcgaacagg gcgatgtaga aacgctgttt
    50041 aacgccccca aacatacggt gacgcgcagc ctggtttccg ctcatctcgc cctctacggt
    50101 atggagctgg catcatgacc ttacttaacg tctgcggcct ttcccatcac tatgcccacg
    50161 gtggatttag cggaaaacat caacatcagg cggtgctgaa taacgtttcc ctgaccctga
    50221 aaagcggcga aaccgtcgcc ctgctgggac gcagcggctg cggaaaaagc accctcgcgc
    50281 ggctgctggt aggcttagag tctccctcac aagggaatat tagctggcac ggcgaatcgc
    50341 tggcgaaact taatcgcgcc caacgtaaag ccttccgccg tgatattcag atggtgtttc
    50401 aggactccat cagtgccgtg aatccgcgca aaaccgtgcg cgagatcctg cgtgaaccga
    50461 tgcgccacct gctatcactg aaaaaatccg aacaactggc gcgcgccagc gaaatgctga
    50521 aggcggtcga tctcgatgac agcgttctcg acaaacgccc accgcagtta agcggcggcc
    50581 agctccagct cgtctgcctg gcacgcgcgc tggcggtcga accgaaattg ctgattctgg
    50641 atgaagccgt ttctaacctt gatctcgtgt tacaggcggg cgtcattcgc ctgctgaaaa
    50701 agctacaaca acagtttggc actgcctgcc tgttcatcac tcatgactta cgcctggtgg
    50761 aacgcttttg ccagcgagtg atggtgatgg acaacggaca aatcgccgaa acccaggcgg
    50821 tgggagataa attaaccttt tcctctgacg ccggacgtgt gctacaaaac gcggtattac
    50881 ccgcattccc cgtgcgccgt cgcacctcag aaaaggttta acgccaatgc aacgagtcac
    50941 catcacgctt gatgacgatt tactggagac gctggacagc ctgagccagc gtcgtggtta
    51001 taacaaccgt tccgaagcta tccgcgatat tctgcgtagt gccctggcgc aggaagctac
    51061 ccagcagcac ggcacacaag gtttcgcggt gctgtcgtat gtgtatgaac acgaaaaacg
    51121 cgacttagcc agccgtattg tttccaccca gcatcatcac cacgacctct ccgtcgccac
    51181 gctacatgtg cacatcaacc acgacgactg tctggaaatc gccgtgttga aaggtgacat
    51241 gggtgacgtg cagcattttg ccgatgacgt tatcgcccag cgcggcgtgc ggcacgggca
    51301 tttgcagtgc ctgccgaagg aagattgagt tatttctaaa acaaggaaag aaaagatatt
    51361 aactgaactt aatttataaa attaagttca gttgcataga tagatcctgt aaaggcagat
    51421 attctgcgta atgtacgtcg ctaaaattta taaatgattc aaacgctcat tcagtgtttc
    51481 aataatatag gtattaacag agacttgttg ctgcgcagcc gcattattaa ggcgttcact
    51541 cagcgattct ggatagcgta acgtaaaggt cttaatcttt tctgtacgcg catacggctc
    51601 aatacccgca gctttacaat cctcaagata ctctcgcagg gaaagctctc cttctttttg
    51661 cagcccctga atactgtcag agacaaaatc acaataacct gacaagccca ggaatttgcc
    51721 acgaaatgca ttcagttctg gcacgtaggt aataacagct ggttgcccgg caatctccat
    51781 agagtttggc gtcttcagtt tgatcatggt tttaccccta tacttagtaa ccagtcacac
    51841 acgctttcta cagcgccttt atcggtatct ggcgacggat gaggccgatg gaaacacgca
    51901 acgctcatat ttagtatgaa cttgcaacgc gaacctcttc cttccttaat ttctccgcct
    51961 aatgctttaa ccagtgactc tatatcagac cacttgatcc cttgaggaac aggagtttta
    52021 aatatttgat ccaatgtatt tttttgtttc ttccgtaaag cctgtacctt ggttctcatg
    52081 gcatgtcctt atgacttcac tttatgacat cacagtatga agtcaagttt cccttcctta
    52141 cgccattgtc ccaatcgtct tcctgaatcg cagcagtgcg atggtgaaaa acgcgccgcc
    52201 aattgccatc agcgtcagga actgcggcca gacgatttcg aatccggcac cccggtagag
    52261 gatggcctgc gcgaggctaa caaagtgtgt cgtcggcatg gtcagcataa tgtcctgcac
    52321 catctgcggc atactttcgc gcggcgtgga tccaccggaa agcatttgca gcggcagcag
    52381 caccagaatc accagcagcc ccagttgcgg cattgaacgc gctatcgtcc ccataaaaat
    52441 gccgattgac gtggtggcaa acagactgag cgccacgccc agcataaaca gcgggatcga
    52501 gccttcaatc ggtacgccca gtacaccttt caccatcagc accagcgata atcccgatac
    52561 caccagcacc accagcccca tcgaccagat cttcgccatc atgatctcaa acggcgttat
    52621 cggcatcacc agtaaatgtt ccaccgtgcc gtgttcacgc tcgcggatca gcgccgatcc
    52681 ggtcaataca atcgccagca tggtaatgtt gttgatgatc gccatcaccc cgccaaacca
    52741 cgcgggatca aggttcgggt taaagcgcat ccgggtttcc agcgatacca acggttcgct
    52801 gttatcacgg tagcgcgcga caaagctgtt cacttcaccg ttgataatat tctggatata
    52861 cccattgccg gtaaatgcct ggctcatgcg cgtggcatcg acgttcacct gaatatccgg
    52921 ctggcgtccg gcgaggacat cacgctgaaa attaggcgga atatttatcg cgaaggtata
    52981 gcgtccggcg tccagtccgg catccatctc atcggcggtg atcatctccg gtggcaaaaa
    53041 ccacggacga tagaagctgt taacgatccg gttcgataac tgcgattgat ccatatcggc
    53101 aatggcgatc ggcgcgaggt tcaacgatcc tggcgtaacg gtcgctgacg aatacaccga
    53161 caccgtaaac gagaagacaa tcagcgtcag catcgcttta tcaccgagca gactgcgcaa
    53221 ctctttgata cccagattaa aaatattgcg taaatggcgc atcatccctc ctgttttttc
    53281 agcagcagga tgcttaagcc catcaccagc gggatggcta tcagcaacgg gataaaaagt
    53341 tgccacaaat cagtcagatc cagcgctttc gagaacgttc cgcgggcgat agtcagaaaa
    53401 tgactggtcg ggtaaacctc gccgatccaa cgtccaggcc cttccagcga agctaccgga
    53461 tcgatcatcc cggaaaactg tgtcgccggg atcaatgtga taatcgccgt tccgaaaatg
    53521 gcggcaatct ggcttttcat aaaggtggag atcagcagcc ccattccagt ggcaatgatg
    53581 atatacagca gcgccgccaa ggtgagcgtc aggaaactgc ctttatgcgg tacgccaaac
    53641 acaaacaccg acaggccgca gagcaggaaa aagttcagca tccccagcgc gatgtacggc
    53701 agctgtttgc caagcaaaaa ttcgctacgg gtggtggggg tcacgtaaag gttgataatc
    53761 gaccctagct ctttttcccg caccacgcta agggcgctta gcattgacgg gatcatcatc
    53821 agcagaagcg ggatcaccgc cggaacaatc gctggcaggc tttttacgtc cgggttatag
    53881 cgataacgcg tctcaatgtt catcagcccg ctttggctgg cgggtgtcga ttgtcggctc
    53941 gccacatcct gaagccaact ctggtgcatt gcctgcacgt aaccttttac cgtttcggca
    54001 cgactgggca tcgctccgtc gatccagacg ccgagttcca caggcgtacc acgcgcgata
    54061 tcgcgcccga aattgggcgg gatctcaatg gccaccgtga tatcgcccgc acgcatccga
    54121 cgatcaagct cgtcataact ggtgagcggc ggctgttcga taaagtaacg ggaaccggag
    54181 aggttgagtg tccacgcctg gctactgacg gtctggtcgc ggtcgagcac cgcaaagcgc
    54241 aggttttcca catccatact gatgccgtaa cccattatca gcatcaggat caccgttccc
    54301 atcagcgcca gcgtcgaacg tactggatcg cgtcgcagtt ccagcgcttc gcggcggctg
    54361 tagctaaaca gacggcgcag gctaaatccc tgacgcggcg cgtgggtggt gtcgtgtacc
    54421 acgggcggcg cttcggcttc gttgctctgc cctgccgctt cctgcaaata ggcgataaat
    54481 gcctcttcca gactggcggc tccgcgtttc tcaaccagtt cctgcggtgt accgctggca
    54541 agcacttttc cggcgtgcat cagtgagatg cggtcgcaac gttccgcttc gttcataaag
    54601 tgggtggaga tgaagatagt cactttgtcc tggcgcgaga gatcgaccat caactgccag
    54661 aacatatccc tcgccaccgg atcgacacca gaagtaggct catcgaggat taacatctcc
    54721 gggcgatgaa tcaccgccac cgccagcgaa agccgctggc gaatgccgag cggcaatgac
    54781 tccggcagaa catcttcaac gtcgttgagc ttaaaacgct cgctcatttc agccactcgc
    54841 gcgggaattt ccgcttccgg gatgtgaaac aaacgggcat gtaactccag gttttgccgc
    54901 acggtgagtt cgttatagag cgaaaacgcc tgcgacatat agcccacacg acggcgggta
    54961 tcgatatctt ttggatcaac cggttgccca aatagccacg cctcaccttc gctggcgggc
    55021 agcagtccgg taagcatttt catggtggtg gatttaccgc agccgttcga accaaggaat
    55081 ccaaaaatct ccccgcgtgg aatgcggaaa ttgacgtgat caacggcaac gaaggaacca
    55141 aaacgcatgg tcagatcgcg cgcttcgatg gcaatctctg cgttttcagg ttgatacggt
    55201 gggatcacta ccgcctgatg cgcctggcgt tgcgcttgcg gtaacagatt aataaatgct
    55261 tcttccagcg tggcgctttg cgtttgctgc cgcagctctt cggcgctgcc ggttgccagc
    55321 acttctccgg cattcatcgc taccagccag tcgaagcgtt cggcctcttc catataggcg
    55381 gtggcgacca gcacgctcat attgctctgc cgctggcgaa tactgtcgat cagatcccag
    55441 aactgggcgc gggagagcgg gtcaaccccc gttgttggct catcaaggat caacagttcc
    55501 gggtcgtgga ttaacgcgca gcacagccca agtttttgct tcatcccgcc ggagagttta
    55561 cctgccggac gatcgcgaaa cggtgctaac ccggtgctgg tcagcagctc attaattcgc
    55621 gcttcccgct ccgctttatc gtgaccgaac aggcgagcga aaaaatcgac attttcatac
    55681 accgacaagg tgtggtagag gtttttgccc agcccctgcg gcatccaggc gatgcgcggg
    55741 cagacgtctc ggcgatgctt cgggtcgtgc atatcgccac ccagcaccac cacattgccc
    55801 tgctcaatga cgcgggcacc ggaaatcagt gacaataaac tcgacttccc gacgccatcc
    55861 ggaccaatca gcccgaccat gcaacgggcc ggaatatcga gagtgatatt gttcagcgca
    55921 acggtttttc cataatgctg gctcacgccc gccagttgcg cgacaggcgg gacgggaacc
    55981 agttccagat gcgtcattgc ggcaacctca ccacgaggtc gtcaggccac ggaagttctt
    56041 cattcacccg cacccacgct acgcccggta aaccggtttt gacatattcc agatgctgct
    56101 ggagtaattc cggtgggata cgcgctttga cgcggaacat cagtttcagc cgttcatcgc
    56161 tggtttcgac ggtttttggc gtgaactggg cgacactggc gacaaaactg atggttgcag
    56221 gaatacgcag atctggcgcg gcatcgagga tcagccgggc ttcaccgccc agtttcagcg
    56281 tgcccgcctg ttcggttggc aggaagaagg tcatatagac gtcgctgaga tcgaccatat
    56341 tcagcacccg accgcctgcc gccagcactt cccctggctc ggcaacccga tactgcacgc
    56401 gtccgtcacg cggggctttc agttcactgt catcgatatc tgcggcaatg cgccgttcag
    56461 tggcttgcgc tgcttcgacc cgagtttgcg cctgaatgat attggtgcgt gctgcttcta
    56521 tagccgcttt agaagccgaa acctgggctt tcgctgattc cagcgcagct cgggcgctct
    56581 cagcggcggc gcggtcgtca tccagctgtt gcgcagaaat agcccctcgt tgggcgagcg
    56641 agcgggaacg cgtatgacgt ttggcaacag agtccagttc ggcctggcgt tgattgacca
    56701 gcgactgtgc ggcacgagtt tcactttgtc gttgctccag caaagcctgc gcggcggcaa
    56761 cggcgctttg tgcctctttg atttgcgcga tggcttccag tcgctgttcc tgcaacacgc
    56821 gagtatccat cttcgccagc acttcgcctt cgcgaacaaa ctggccttct ttcaccagaa
    56881 tggtgtcgat acgcccggca attttgctgg cgatatccac ttccgtcgct tcaatgcgcc
    56941 cattgctgac agcaaagcct tccggcacac ccgccgggcg caacagccac cacgccacga
    57001 cggcagccac cgccagtgcc ccgacaaccc accacgccag atggcgctta ctcttatcca
    57061 taatcgaccc gccataatcc ctgtaagcaa acgacgcgtg ttacaactgc cgccgaacga
    57121 cgttcagcgg attcttccat ccgggataag aaggaaacga caaatacgac cagcgcgcag
    57181 agcattgagc gccaacgggg aaggcttagt gaaaacgggt agtaatgttg tcatcatcag
    57241 cattcctggc cgtaaatgaa acacgcatac tcataagtgt gatctataaa actggctgac
    57301 aaaacggtat ccatcctgtt ggatcgcatt tcacgcctgt tcagttgcag gaacagagca
    57361 ttccttttta cgttttatca acagtctcaa ccagatagta acattaatat tcatgttgtc
    57421 tatggttcag agctgtaaca ttgtgcgcct tctaatcaaa ggcataagtc catttctgtt
    57481 tttttcatgc tggtaagact tccagggagt ggttatctct ggccctgcac cagggttaat
    57541 ttggcaatta tgcatgctga acattcttcc agcatgttta ctgtcacggg cgttaagacc
    57601 taaaactgtc cccgtttcac ctatgcgttc tctcccacca tttccgttat gtgcaacaac
    57661 ttgatttata cctaacattt gtgaacatgc taatagtgct tcaagtttag gtctgtaatc
    57721 tgtcgaaaca gaaaaataac gattaaagga tcgacaagag atagcccggc tagttttaaa
    57781 ctttttgcca ttaattatag catgatgctt tttattaata atttccgcta attcgaccgg
    57841 atcgagagca ttggtcattt catcaataga gacagttatc ggtcctataa tgtaacgttt
    57901 tcgctgatca tcaaaagtta tcccatgatg atttgccatt attttgtatt tggggtcata
    57961 gaaacataca ggataatctt ttatcccgtc gtaggtatcc tcatcaagtg gtttcatgcg
    58021 cgtgaagttt tgcatatagt taccattgca attcgtttca tgatttccag ctaatacatg
    58081 aacattttca ttgatactca ttatgcgttt taacaaatcg atgataaatt gatcgccaca
    58141 gatattacta aatcgatcgc cggtttggtc gccaagaaac gtacatggcg tgactgcgtc
    58201 tttattaata accaaataag gctgaagact ttctaaaaca ttatctcttc tgttcagtat
    58261 actgattatt gtattaacat tagtgtcaag ttgctcatta aagttctccc ttagcaatac
    58321 gttgagaact tcatcttcta tattaagcaa ctggctaagg aaattttctc cttcagccat
    58381 gattcccaaa tgtcctgttg caaaaagcgc gtacaaaact gcaccgactg agccatcggt
    58441 atcgccaaaa taggtcggcc tggtacttgt gggatcaaca ggtgaacaat tggagaaata
    58501 ggttctgtca gcgaactggg gttttataat tgtttcgaat ttaatatcaa atacattgta
    58561 ttgcgatatt tcccagttcc agatgggttt gtacgactca gtaactgcaa catcattagc
    58621 attatcattt actgtcatat tattaagtaa ataaacaatg ctgcaccaat taatatcggt
    58681 attatcaaga gggcaaccgt aatttttact attaattgtc acaataaact gataacagtt
    58741 attcctacgt agaatatgag cgcgcatgcc cgatagcata ctatattttt ctgaatgatt
    58801 cgtttgtatg gtaaatgaaa ttttgtcatc ttgaatagat tgttggaaac gttctatttg
    58861 ccaatcatca attttttcca caacttcacc gagcattgtc ctggcaatac ttgatatatc
    58921 atcacctata ccatttgcaa tatacccaaa cagttcttta gtgaagttgg taacatgctc
    58981 ttgcaccata tcatttgttg cgattgtcgt tgtactgcca ttagtgcctg ccacagttcc
    59041 aattttcatc ttttacctca tattattcac cgcgaggaat acatagttaa cattttatct
    59101 tatgacatac acctcatatt cgatggggaa ataattatat tttgcacaaa tcattcagcc
    59161 ttaaagcata atatgaaatt tcttatctca tagcagagaa agtccctaaa tattgtagag
    59221 gcgtcatcag tacatacctc tacaatcgat taagaaaacc tatgaaatac tggcaaaatt
    59281 aaaaagagat accacagcac tcatgaagtt ttattgaggt tgaaaacaac taagccctgg
    59341 gacacctttt ttatcaccac gttcgttagg tgctatcttc caagaattta gccccatggt
    59401 ataattatca attttttgat gctggtctac ccctaatcca taatggccaa atttctgttt
    59461 agtttttttg agcgtctgtc cacatttgaa gatatcttca ggttttggtc tgaaagcagt
    59521 agtgttatta aaataatttt catatacagg tgtgggtgct atagcattgc ttctaattag
    59581 atggagaaga tgatcatcca tgtgttgttt atgtttttta ttacatatat ttgctaattc
    59641 ctcaggatca attgggtctt tcatctgatt aaatggaacc tgcaaagcac cgagacaata
    59701 acattgattt tcttcatttc ttattattcc gtgatgactg gttaaaacct ttagtttctg
    59761 agttataatt acaaacatcg agcgttttaa ttaagttata cgtatcaccg gcagagagtt
    59821 tataattttc tagtcgtgca gtatagttac cattaaaatt gatttcatga tttccagcca
    59881 gaacgatgac atttttatta atacggctat ctttattacc ctccatattt cgcatagaat
    59941 ttagcaaagt aaggatatat ttatcaccaa aaatagtact aaatctatcg cctgtatgat
    60001 ccccaaggaa gatacaaggg gttatcgcat cgtcctttaa aaaaacatgt tgatgcaact
    60061 cattcaatat catgtctctc gtatttaata aattgtagag caatttatta tttttatgta
    60121 tgtatgcaga tgaagccatg tcttcgtgtt ttaataattc acataataaa ctccaaccct
    60181 tttccctgat gcctatatgc cctgaaacga gcaatgaata aagtacagcg cctactcttc
    60241 catctgtatc gccaaaataa gccgtgttgg cattttgtgg gttaatcaca ccacattgtt
    60301 tataaaagtc tattttagaa aacacagatt taacatagct atcaaactta tccttaggaa
    60361 acgtctcttt agagacatcc caacgccagt cagctttaaa attaccgcta agaagagtaa
    60421 agaaattttc aggtatgata gaaagcccat taactttata aattatgtct gatttatctc
    60481 cggagaaatt aataaaataa taaccatcct tacactcaat cttaactgta atgggagacg
    60541 ataattttat cacatggtta ttgtctggta taattgtaaa gataaacgtg ttagtagtat
    60601 tatcggcaaa actattatat tcttgaatat ctatagatgg ctcactattg taaaaccata
    60661 ttttctccat ctgaagacta tgttcattta ccctccagtg accatgttgc aggaaataga
    60721 tgatatgcat gccaaaatcg ttatctattt gcgaatcatt atccagctta tcaatttcaa
    60781 ctatctttga acgatgtgtt ttatcaacct tgctatggtg ttgatgatta ttaactttat
    60841 ctgaatgctt atctttatgg tttgaaggga tgtacttaaa tatattgtca attgctttca
    60901 taaataaccc gcgatatttt tgacctgtta gtaaaaaata aatgatacat gtctgaaaga
    60961 catatttacg acgatataaa acaataatac ttatatgttc gctgatttta agcaccgaga
    61021 ctatccggtc ttttactaaa taatggaaaa caatccatcc ctggaagaca attgtaaaaa
    61081 aaacaacaga ataagaatta atcttaggat aaatatttat ttatcatggc cttttgaccg
    61141 ctaaaaaatg gcactcggca cagtaaaccc cgctatagtt atgctatgca tttgtcttta
    61201 atgatatctc gtcgttatat taacaggctg aaattatcat taatgcacat ttcaatatta
    61261 gcagggatac ctgaggagtt taaagtgaac atatatatcg ggtagctttt caaattaatc
    61321 cctttgatta taggcttaat ttgcatcgcg ttaggtggct ttgtgctgga aagttcaggg
    61381 caacgtcacg ggtcatgtgc tgatttctct ggcggccata tgcctggcat tattcactac
    61441 cgcatttatt atcatttcgc aactcacgcg cggcgttaat acgttttaca atacattgtt
    61501 ccccattatt ggctatgcgg ggtcaattat caccatgata tggggttggg cactgttagc
    61561 aggtaatgat gtgatggcag acgagtttgt tgccggccat gttatttttg gcgttggtat
    61621 gattgccgca tgtgtatcga cggtggcagc gtcgtccggt cactttctgc tcattcccaa
    61681 aaatgcagcg gggagcaaga gcgacggaac accggtacag gcttattctt cattaatcgg
    61741 taattgcctc attgccgttc ccgttttact caccctgctc ggtttcattt ggtctattac
    61801 gctgttacat agtgccgaca taactccgca ttatgtcgcc ttgggttaac cgcaatctgt
    61861 gcctgtctaa ttggcctagt ggccacaatc gtccatcaaa cacgtaatac gttctcatcc
    61921 aaagagcact ggctgtggtg ttattgggtt atttttctcg gctcagtcac ggtgctgcag
    61981 gggatatacg tcttagtcag ttccgatgca agcgcccgac tggctcccgg cattattctc
    62041 atttgcctcg gaatgatctg ttacagcata ttctcaaaag tctggctact ggcactggtc
    62101 tggagacgta cctgttcgtt agccaacaga ataccgatga taccggtatt tacctgtctg
    62161 ttttgcttgt ttttgtcatc attcctcgcg gaaatcgcac aaactgagct ggactacttt
    62221 attccatctc gggttctggt tggattaggc gcagtatgtt ttacattgtt ctctattgtt
    62281 tcaatattag aggccggttc tgcgaaaaaa aattaaaatt tacataatcc cagcgttatt
    62341 catatcttaa cgctggtttt ttacgaactt agtgaaataa tgtagaatat aaaattataa
    62401 ttttatattg gagcacatgt gttaatgatc aaccccggga tactaaaaat atagacatta
    62461 ttaaacttgc ttagcctcgc attgccttta cctgttaaag atgcatcgag aaaattgagt
    62521 ctgaagttct catcctttat ctgattttcc agtcccgaaa ctttggcctt agcattacct
    62581 gagatccgtc cgttactatc tattttgtag gtaaaaaaat acgaatggga aaatttcctc
    62641 ggtgtatctt catcagttaa gccaacaatc gtaaaactac cgctaccatc tttttttaaa
    62701 atgactgaaa ggttagcaga gtagtataaa gaatgatctg ttttagcatt tcttgttaac
    62761 gtcgacgtac attcaagaga cgttgtgcta ccatcacgag ccttccagag agcaaaaaaa
    62821 cacacctgta gcaatgtgaa aacaagtatt aatataaaat acattttctt attagtcata
    62881 taatagcacc tctgaacaaa ttgcataagt ttcgtcatta gaggatttta tacatcttga
    62941 taaataccgc tgcccttccc caagcaaata atgcatacta tttacatcat atataaaatt
    63001 tgcacctggc acacagggta aatgcttttt cgaaatttca gcggcctcta tttgagattt
    63061 agcgatgcca atctcgttaa gattctctga aagatataaa tcacaggtgt caatcttacc
    63121 tgatttaacc aatggataat ccgttatact gtaggtatct ttaaaagcca cggcaaacat
    63181 ttcaataata acaattacta ccacaagcga aatcaaaatg cgaaaaagag gaatactcct
    63241 tttgcgctct actttttcaa caacatcttc ttgtaaatga gaatcatcat aagattgttt
    63301 acttattgtt atgttttcac tgatgtaaaa gcctactttg ggtatcgtaa gaatcacatt
    63361 ttcttcaaca ccaatatttt taaaactttt gcgtaacagg gatatatatt ggttcaatgt
    63421 gttgtttgac agatcaaatg aaaatctctc caaaacgcta tcaatgattg agttcctgtc
    63481 aaccgctcca ccattacttt ccagtaacac tgccaaaata caccccgccg tagctggaaa
    63541 atttatctca tcctgggtat ttacattcga gagcgagcat ttgttcggat caaaaataat
    63601 cgcgtcattg ataatgaaaa aacatctccg ctccatgcag gcaacaacac aaaataagat
    63661 tttgtcattt tagcctattc ctggtggaac tgacacaaga aactaaaata caaatataac
    63721 cctgaaaaag tcatataaaa cttatatcca cagaataaaa aatcaaacat gtcagtttta
    63781 tcaatataaa gtatcgcaag gcgaaaagtg atgcaagaat actcctaagt aaatgaaagc
    63841 gcaccacatg aaattatcaa cacatgtaat ttcatcatta ggtgctatcg aaaataactc
    63901 aggagctact taagatgaat aatgtcttac tcatgagacc cgatgactca gaatttatga
    63961 tacctgatga ctggtcaggg ctaaacgcaa aaggcattac atacgaacaa gataatagag
    64021 tgacagagtc ttttctgcct tttaaatctt tttattgttc accggtgtgc acgacagttg
    64081 aactcatctc ataaaataat tgttttgcta tgggtatccg ctaccttctg gaaaagaaaa
    64141 gagacttaag gctaaatcac tttaaagcct tctctcataa atgcgagaca aaacctgacg
    64201 tgttaattat gaccgggtgc accgtcgctg atacttatag tatattctca cgcattgttg
    64261 aagaaaatat aatgaaaacg atgacaccta tcgtcatcat tactgggcat cactcagaag
    64321 atctttgttc tctggcatcg aaactgggag gagttaaaat aatatcaccg acagaaaaga
    64381 ttgcaaaaat agaaaacgct atcaatgatg cttttaacag caagcctaaa aaaataagat
    64441 ttttaacaga tatactctct cctcaacaaa ttgagaccat gaaaatggtt gcgcagggta
    64501 gatccgttaa tgagatttca gccgcaatga atattagcat caagaccgtg tatgcacata
    64561 aagttcgtgc tatcaataag ctgggaatta agaaaaaagt ttatgaagcg cttttttata
    64621 aagcgctaat tcagttcaac tgatgtaaaa atcaccctcg cttaataaat aatatattct
    64681 atgtcttttt ttataagtta aaaaaggcgg gatatcaaat cccgccatga aattaactaa
    64741 tcatctgcat tattctgcgt agatataagc ctgggtacgg atggtgtaag agtctggctc
    64801 aacaacctgt tctttcatat gggaaacgac gcgataggca ccacctgttc cagtattttt
    64861 cagcatcata taacgcttac cgccaatctc ttcggaggca gctgaaggag gatcctgctc
    64921 aagcgttgtc agggaaactt tcagtttatg gtccgggtta ttgtcaccag aaattactgc
    64981 atccgctgaa gtactgctct ccgcataaat atcatccggg aaagttacag catagtcgtg
    65041 gctctgccca tcagatgcgc caacaatacc tgttgccaaa ggcgtccatg cagttaacgt
    65101 accgctatat acccctttca ctggttgaat ggtattatta acaacgatag gcttggtcaa
    65161 agtaagatta gctgacgctt cagtaccctg gcttactaaa gttcccgcct gagaagcaaa
    65221 agctgtcccc gccagcatga ttgctaacaa tgttttcttc attatatttt ctccatgatt
    65281 ttcatttaca ctgcaaaatg cagtttcttt ctttgcattt acgtgctatc gcgaaaatgc
    65341 ttccaccaaa taatggaaac atcttcacaa aaataaatta tgagtttctt aatttagaaa
    65401 ctataaacat gtcgccgcta tctggtataa ccctgatgca cttttcttcg caggaagttg
    65461 gtattttgcg atacaggatt gatctttaga ataaccccat ttcacattaa actcgccttc
    65521 cgcactcaag ccagaaacat aagcaatacc atcttcacct acaatgccgg ccatattttc
    65581 agtgtctttg ctataactaa tcacagcgcc gaaggggatg ggtttaccct ctttctgttt
    65641 taaggttact aatcctttat ccccgctctt cactgccaat gtcgcaggca caacggctcc
    65701 agctgtcggt acaaccaatt gactagtttg tggtaagtcg taattcttac ctatcgtttt
    65761 ttcatccaga ataacatcgt ctcttcggta tggctgcact ggcagtattg catacccacg
    65821 aaaatcagta ctcagtcccg gccagccatt aacgttaacc cccgttgctc cgggtgcctc
    65881 tataagggct aatgtcccat ccacattttg accaaatgta agtccatccc ttgtaataag
    65941 tatcccaccg cttaaatttg cacctagctg tttcatgttt tcgctggagc tgtaatggct
    66001 actaaactga ccatatgatc catttttgcc aacttgcaca atgttcatcg tgctcttcgg
    66061 ttgtgactga tgagcgttag ttatatttgt cgctacatcc caccatacac caccactatt
    66121 ctgacctgat aatgaaacct gatagttatt accctgctct cccgaacgac tcatctgtga
    66181 agacgcataa atttgatttt cgtcacccag cgataatggc actgaaatac ccacgcttaa
    66241 tttccgatct actttccatt tatgcgatgt cagcaagtcg ttttttgccc agttaatgga
    66301 gaatgttatg cctctccata ccgtactata actcaggctg gtagaccgcg tgtttcctgt
    66361 gccccgggaa gaattatacc tcgtggtatt gaagttcaaa ttcccgatat taccaagggg
    66421 ctgggataag gatatcgtag agccatcgtg actattctta tctctttttc tgttccgttc
    66481 atcctgtgca cttactgctt cagtcagtgt ggcataacct gatgcataac ggttcgtggt
    66541 tatagacaat agactggccc cctgaatctc cagacaacca gtatcactta tttaagtgat
    66601 agtcttaata ctagttttta gactagtcat tggagtacag atgattgatg tcttagggcc
    66661 ggagaaacgc agacggcgta ccacacagga aaagatcgca attgttcagc agagctttga
    66721 accggggatg acggtctccc tcgttgcccg gcaacatggt gtagcagcca gccagttatt
    66781 tctctggcgt aagcaatacc aggaaggaag tcttactgct gtggccgccg gagaacaggt
    66841 tgttcctgcc tctgaacttg ctgccgccat gaagcagatt aaagaactcc agcgcctgct
    66901 cggcaagaaa acgatggaaa atgaactcct caaagaagcc gttgaatatg gacgggcaaa
    66961 aaagtggata gcgcacgcgc ccttattgcc cggggatggg gagtaagctt agtcagccgt
    67021 tgtctccggg tgtcgcgtgc gcagttgcac gtcattctca gacgaaccga tgactggatg
    67081 gatggccgcc gcagtcgtca cactgatgat acggatgtgc ttctccgtat acaccatgtt
    67141 atcggagagc tgcccacgta tggttatcgt cgggtatggg cgctgcttcg cagacaggca
    67201 gaacttgatg gtatgcctgc gatcaatgcc aaacgtgttt accggctcat gcgccagaat
    67261 gcgctgttgc ttgagcgaaa acctgctgta ccgccatcga aacgggcaca tacaggcaga
    67321 gtggccgtga aagaaagcaa tcagcgatgg tgctctgacg ggttcgagtt ctgctgtgat
    67381 aacggagaga gactgcgtgt cacgttcgcg ctggactgct gtgatc
//
LOCUS       DAJTRN010000007        67371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRN010000007 DAJTRN010000000
VERSION     DAJTRN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..228
                     /locus_tag="OO328_000473"
     CDS             <1..228
                     /locus_tag="OO328_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5929159.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO328_000474"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO328_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO328_000475"
     CDS             1365..1688
                     /locus_tag="OO328_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5929160.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO328_000476"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO328_000476"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5929161.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO328_000477"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO328_000477"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5929162.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO328_000478"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO328_000478"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5929163.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO328_000479"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO328_000479"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5929164.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO328_000480"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO328_000480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5929165.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO328_000481"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO328_000481"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5929166.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO328_000482"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO328_000482"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5929167.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO328_000483"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO328_000483"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5929168.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO328_000484"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO328_000484"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5929169.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO328_000485"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO328_000485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5929170.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO328_000486"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO328_000486"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5929171.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO328_000487"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO328_000487"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5929172.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO328_000488"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO328_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5929173.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO328_000489"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO328_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5929174.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO328_000490"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO328_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5929175.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO328_000491"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO328_000491"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5929176.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO328_000492"
     CDS             complement(21226..23058)
                     /locus_tag="OO328_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5929177.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO328_000493"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO328_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5929178.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO328_000494"
     CDS             complement(23806..24300)
                     /locus_tag="OO328_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5929179.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO328_000495"
     CDS             complement(24383..24838)
                     /locus_tag="OO328_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5929180.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO328_000496"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO328_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5929181.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO328_000497"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO328_000497"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5929182.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO328_000498"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO328_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5929183.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO328_000499"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO328_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5929184.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO328_000500"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO328_000500"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5929185.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO328_000501"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO328_000501"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5929186.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO328_000502"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO328_000502"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5929187.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO328_000503"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO328_000503"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5929188.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO328_000504"
     CDS             33414..34307
                     /locus_tag="OO328_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5929189.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO328_000505"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO328_000505"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5929190.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO328_000506"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO328_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5929191.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO328_000507"
     CDS             complement(36223..36939)
                     /locus_tag="OO328_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5929192.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO328_000508"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO328_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5929193.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO328_000509"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO328_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5929194.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO328_000510"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO328_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5929195.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO328_000511"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO328_000511"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5929196.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO328_000512"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO328_000512"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5929197.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO328_000513"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO328_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5929198.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO328_000514"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO328_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5929199.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO328_000515"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO328_000515"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5929200.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO328_000516"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO328_000516"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5929201.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO328_000517"
     CDS             complement(45788..46447)
                     /locus_tag="OO328_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5929202.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO328_000518"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO328_000518"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5929203.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO328_000519"
     CDS             complement(47342..48355)
                     /locus_tag="OO328_000519"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5929204.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO328_000520"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO328_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5929205.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO328_000521"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO328_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5929206.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO328_000522"
     CDS             complement(50648..51826)
                     /locus_tag="OO328_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5929207.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO328_000523"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO328_000523"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5929208.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO328_000524"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO328_000524"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5929209.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO328_000525"
     CDS             complement(55608..55886)
                     /locus_tag="OO328_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5929210.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO328_000526"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO328_000526"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5929211.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO328_000527"
     CDS             complement(56468..57277)
                     /locus_tag="OO328_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5929212.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO328_000528"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO328_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5929213.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO328_000529"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO328_000529"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5929214.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO328_000530"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO328_000530"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5929215.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO328_000531"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO328_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5929216.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO328_000532"
     CDS             complement(60978..61868)
                     /locus_tag="OO328_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5929217.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO328_000533"
     CDS             complement(61855..62379)
                     /locus_tag="OO328_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5929218.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO328_000534"
     CDS             complement(62376..62846)
                     /locus_tag="OO328_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5929219.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO328_000535"
     CDS             complement(62843..63391)
                     /locus_tag="OO328_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5929220.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO328_000536"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO328_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO328_000537"
     CDS             complement(64138..64860)
                     /locus_tag="OO328_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5929221.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO328_000538"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO328_000538"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO328_000539"
     CDS             complement(66023..66778)
                     /locus_tag="OO328_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5929222.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..>67371)
                     /locus_tag="OO328_000540"
     CDS             complement(66860..>67371)
                     /locus_tag="OO328_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5929223.1"
                     /translation="TSTATIAAGNNGTARFYGTIEDSVCSIVPDDHKLEVDMGDIGAE
                     KLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTDTGAAFNN
                     VSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFNAWLVGAADAPDLGNFEA
                     NTTFQITYL"
BASE COUNT        16817 a        18227 c        16736 g        15591 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagca gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttacggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg a
//
LOCUS       DAJTRN010000008        67022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRN010000008 DAJTRN010000000
VERSION     DAJTRN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO328_000541"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO328_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5929224.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO328_000542"
     CDS             complement(1151..1237)
                     /locus_tag="OO328_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5929225.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO328_000543"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO328_000543"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5929226.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO328_000544"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO328_000544"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5929227.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO328_000545"
     CDS             3586..3630
                     /locus_tag="OO328_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929228.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO328_000546"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO328_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5929229.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO328_000547"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO328_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5929230.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO328_000548"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO328_000548"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5929231.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO328_000549"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO328_000549"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5929232.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO328_000550"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO328_000550"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5929233.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO328_000551"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO328_000551"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5929234.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO328_000552"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO328_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5929235.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO328_000553"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO328_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5929236.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO328_000554"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO328_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5929237.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWLEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO328_000555"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO328_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5929238.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO328_000556"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO328_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5929239.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO328_000557"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO328_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5929240.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO328_000558"
     CDS             complement(16981..17745)
                     /locus_tag="OO328_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5929241.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO328_000559"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO328_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5929242.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO328_000560"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO328_000560"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5929243.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO328_000561"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO328_000561"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5929244.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO328_000562"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO328_000562"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5929245.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO328_000563"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO328_000563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5929246.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO328_000564"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO328_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5929247.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO328_000565"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO328_000565"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5929248.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO328_000566"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO328_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5929249.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO328_000567"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO328_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5929250.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO328_000568"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO328_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5929251.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO328_000569"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO328_000569"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5929252.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO328_000570"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO328_000570"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5929253.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO328_000571"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO328_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5929254.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO328_000572"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO328_000572"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5929255.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO328_000573"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO328_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5929256.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO328_000574"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO328_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5929257.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO328_000575"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO328_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5929258.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO328_000576"
     CDS             complement(40421..41815)
                     /locus_tag="OO328_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5929259.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO328_000577"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO328_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5929260.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO328_000578"
     CDS             complement(42158..43444)
                     /locus_tag="OO328_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5929261.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO328_000579"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO328_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO328_000580"
     CDS             complement(44451..45221)
                     /locus_tag="OO328_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5929262.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO328_000581"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO328_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5929263.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO328_000582"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO328_000582"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5929264.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO328_000583"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO328_000583"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5929265.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO328_000584"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO328_000584"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5929266.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO328_000585"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO328_000585"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5929267.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO328_000586"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO328_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5929268.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO328_000587"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO328_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5929269.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO328_000588"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO328_000588"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5929270.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO328_000589"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO328_000589"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5929271.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO328_000590"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO328_000590"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5929272.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO328_000591"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO328_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5929273.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="OO328_000592"
                     /gene_synonym="lytB"
     CDS             complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="OO328_000592"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5929274.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="OO328_000593"
     CDS             complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="OO328_000593"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5929275.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="OO328_000594"
     CDS             complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="OO328_000594"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5929276.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="OO328_000595"
     CDS             complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="OO328_000595"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5929277.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="OO328_000596"
     CDS             complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="OO328_000596"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5929278.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66109..66327)
                     /locus_tag="OO328_000597"
     CDS             complement(66109..66327)
                     /locus_tag="OO328_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5929279.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66430..66693
                     /gene="rpsT"
                     /locus_tag="OO328_000598"
     CDS             66430..66693
                     /gene="rpsT"
                     /locus_tag="OO328_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5929280.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16259 a        18246 c        17253 g        15264 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcttga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgctgc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttttat gaagaaaaca tgccggtaac aagaccggca ttttcgcata
    60301 acttaggctg ctaatgactt aatcgacttc acgaatatcg acacgcagct ctttcggcac
    60361 ttcgaaaaca atattttctt cacggccttc cagcggaatg gcttcaccac cacccaactg
    60421 ctgcaaacgt gccaccacat tctgcaccag aatatccgga gccgatgcgc ccgcagtcac
    60481 gccgacgcat ttaacctctt tcacccactc ttcctggata tctttcgcat cgtcaatcaa
    60541 aaacgcgtgt ttgcccatac gctgggccag ctccgccaga cggttggagt tggaggagtt
    60601 tttcgaaccg accaccaaca caacttccgc ctgttctgcc agggcgcgta ccgcttcctg
    60661 acggttagtc gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt tcgggaagcg
    60721 tttacgcagc gcgtcgatca catcagacgt gtcatccacc gacagcgtgg tttgggtcat
    60781 aaaggagagc ttctcttcgt ttttgaccgt cagtttccac acatcgtctg gcgattcgac
    60841 tagatacatt cccccttcgg ggttgctgta ctgccccatc gtcccttcca cttccgggtg
    60901 cccggcgtgg ccgatgagaa tagattcttc gccacggcga ctggcgcggg caacttccat
    60961 atgcactttg gtcaccagcg gacaggtggc atcaaacacc gtcaaatcgc ggcttttcgc
    61021 ttcgttacga accgcctgag aaacaccgtg tgcggagaaa atcaggatcg cgccgtccgg
    61081 cacttcgcta atctgctcaa taaagatagc cccacgctcg cgcaagctat cgaccacgta
    61141 gcggttatgc accacttcgt gacggacata tatcggtgcg ccgtaaatgg ccagcgcgtt
    61201 ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa ccacgcgggt tggccaacag
    61261 gatctgcatg ttacgcctcc agtgccggat cgatttccag cacttcaata tcaaaatgaa
    61321 cggtctgccc ggccagcgga tggttgaaat caacggtaat ggagtcgccg ttaatttcgc
    61381 ggatcacacc aggcatctca ctgccatcca ttgcggtaaa aagcatgatt gcgccaattt
    61441 ctggctcgcc tgcatccata aattcacggc gggagaagta ctgaatcagg tccggtgacg
    61501 gcacgccaaa cgccgcatct ggctccagcg agaaggtggt tttgtcgccc actttcagcc
    61561 ccagcaggtg ttgctccagc ccttcagaaa gagaagcatc gcccaggcgg aacagcgccg
    61621 gtttaccgtt gttgcgagta gactcggcgg tggtgccatc gtcgagtttt agcgtgaagt
    61681 gcaccaggac ggcgctattg ctctgtacag attcagacat gcaggtttgc tcttattttt
    61741 gaggattttt gccggatgcg gcctacgatt tcgtagcaat ctgtaggccg catccggcag
    61801 gatttattat tgttttttcg ctttggaagg taaaaaccct tccagcacga tcagtgccgc
    61861 accgacacaa atggcagtat cggcaaggtt gaaggtggcg aagtgccagt cgccgacgta
    61921 gaagtcgatc atatcgacaa cgaagccgtg ccacaggcgg tcgaacaggt tgcccagcgc
    61981 gccgccaata atcagcgcgt aagcgatatt gtttagcttc tgcgtggcct tcgagcgata
    62041 catcatcact gccaggagca cgctaatacc aatcgcaata ccggcaaaga accaacgctg
    62101 ccagccgccg ctatcggcaa ggaaactaaa cgccgcgcca tagttacgcg cataatgcag
    62161 attaagcgac gggaacagcg ggaccgtatc ccccagagca aagttctgga ggatcaggta
    62221 tttgctgccc agatcgataa tcagcacgac taccaccagc cacagccagc gtagccctgt
    62281 tgaacagatc gattgactca tcaggcaaac ttacgttttt caccgtcacc ggcgacgttg
    62341 ctgacacagc ggccgcagat ttctgcgtgt tccgccacct tgccgacatc ctgggtgtag
    62401 tgccagcagc gtgggcactt ctcaccttcg gctttactca acgcgacttt cagccctttg
    62461 agtacttcgc tctgctgagc atcagcaggt gcgtcgttat agtctgcaac ggtagcgccg
    62521 gaggtcaaca ggacaaatcg taattcatcg cccagcgcgg tcagtttcgc cgccagttcc
    62581 ggttctgcat acaaggttac tgccgcttcc agcgagccac ccactttctt gtcggcacgc
    62641 gcttgctcaa tgactttgtt cacttcgcca cgcactttca acagctcgtc ccagaacgca
    62701 tcgttcattg cttcactgtc tgccagacca aacaggcctt cgtaccactc accggtgaag
    62761 acgtattttt cacgttcgcc cggcagatag ccccacactt catcagcggt gaaggagagg
    62821 attggtgcca tccagcgcac cagcgcttca gcgatgtgat acagcgcagt ctggcagcta
    62881 cgacgcgcca cgctatccgc tttggcggtg tactgacggt ctttgatgat gtcgaggtag
    62941 aaggaaccca tctcaacgga gcagaagcgc atcagacgct gtaccacttc gtggaaatcg
    63001 tatgcttcgt acgccttgag gatgtcttcc tgtgccgctt tcgcacaacc tacggcccag
    63061 cgatccagta ccaccatctc ttccggtttc accatatctt ttgctggatc aaaaccattc
    63121 aggtttgcca gcaggaagcg cgcggtgtta cggatacgac gatagctatc ggcagcacgt
    63181 ttcaggatct cgtcagaaac ggccatttca ccggtgtagt cggttgatgc cacccacaga
    63241 cgcagaatat ccgcgcccag tttgttcatc acatcctgcg gcgaaacggt gttgccgatg
    63301 gatttagaca tcttgcgacc ctgaccatcc acggtaaagc cgtgggtcag tacctgacga
    63361 tacggcgctt tgcctttcat cgcggtggag atcattaggg aagacatgaa ccaaccacgg
    63421 tgctggtcag aaccttccag atacatgtcc gctgcgtgac cggcaaattc cggacgcacg
    63481 tcaacaacag aagagtgggt agatccggag tcaaaccata cgtcgagagt atccggcact
    63541 ttcacatact ggtcagcttc gtcgccgagg atctctttcg catcgagatc ccaccacgcc
    63601 tggatgccgt caacttcaac gcgtttagcc acttcttcca tcagttcgag ggtacgcgga
    63661 tgcagctctt ccgtgtcttt gtgcacgaac agtgacatcg gtacgcccca ggtgcgctga
    63721 cgggagatac accagtcagg acggttagca accatcgact cgatacgcgc ctggccccag
    63781 tccgggagcc actgcacgcc tttgatctct ttcagtgact gcgcacgcag acctttctga
    63841 tccatgctga cgaaccactg cggtgtcgcg cggaagatga tcggcgtttt gtgacgccag
    63901 cagcacggat agctgtgctg cattttctca acgtgcagca gcgcgccttt ttcctgcagc
    63961 agcgcaacga cgatgtcgtt cgctttgaag acgttcacgc catccagcgt cggataagtg
    64021 cccggcagat aagtgccgtc cgggccaacc gggttagcgg tttccaggcc gtatttctga
    64081 ccgatcacat agtcgtccgg gccgtggcca ggcgcggtgt gaacggcacc ggtaccggca
    64141 tccagggtaa cgtgatcgcc gaggattgcc ggaacgtcga agcccataaa cggatgggta
    64201 aagcgcagca gctcaagctc tgcacctttt accgtgccga gaatggtgta atcggtcacg
    64261 ccgatacgct gcattacgct ttcaaccaga tctttcgcca gaatcacggc ctgaccgtcg
    64321 atctgcacca gcgcatagtc gaaatcaggt gcaatagaga ttgcgcggtt cgcaggcaga
    64381 gtccacggcg tagtggtcca gatcaccagc gagattgggc cgttaacgtt gcttacgcca
    64441 aatttcgctt tcagcgcatc ctgatcgacc gcctggaaag cgacgtcaat ggacggagaa
    64501 gttttgtcgt aatactcaac ttccgcttct gccagtgcag aacggcagtc tacgcaccag
    64561 tgcaccggct tcgcgccttt gtgcaggtga ccattgccga tgattttgcc cagcgcgcgg
    64621 atgatgttgg cttcagtttt gaagtccata gtcagatacg ggtgcgacca gtcgcccagc
    64681 acgcccagac ggataaagtc tttgcgttga ccgtcaacct gggtcgccgc gtattcgcgg
    64741 cacttggcgc ggaactcggc ggcggtgaat ttctcacccg gcttaccgta ttcttgctct
    64801 actttcagct cgatcggcag accgtggcag tcccagccag gcacatacgg cgagtcataa
    64861 ccggaaagcc ctttggactt cacgataatg tctttcagaa tcttgttaac cgagtgacca
    64921 atatgaatgc tgccattcgc ataaggaggg ccatcatgca gaatgaaggt ttttttgcct
    64981 tttttagccg cacggatgat gccgtacaga tcatcatcag tccaacgcgc cagcattccc
    65041 ggttcgcgct tggcgagatc gccacgcatc gggaaccctg tttccggcaa attcagggtt
    65101 gatttatagt cactcatcag attctcggtt ccgtgtttcg gtttgattac ataacaggct
    65161 taagccggtt ttgttagccc aaaaaattcg cgggcggtta attcatcacg cgcaatctgc
    65221 gctttcagtt cgtccagcga cgcaaatcgc tgctcattgc gtattttttt acgcagcact
    65281 acttgtatat ggcgaccgta aaggtccatt gcaacatcta acaaatgcac ttccagctgc
    65341 tggcgaatac cggcaaccgt tgggcgtgtt ccgatgtttg ccacgccggg taacggcttt
    65401 tcaccgaggc ccagcacttc taccgcataa acccctttca ccggggaaac ctgacggcgt
    65461 agcggtacat tcgccgtcgg gaaacctata gtgcgcccta attcatcacc gtggactaca
    65521 cgcccggaga tggcaaacgg gtgccccagt aaactttctg ccagagccag attgtcatca
    65581 gccagcgcct gacgcacggc ggtgctgctg atacgcacac caccttcgca aaaagtttgc
    65641 gtactggtga tatcgaagcc gtattccatg ccagctttct gtaataacaa gaaatcgcct
    65701 tcacgaccag cgccaaagcg gaaatcatca cctacggcaa gaaattttac gcgcaaatgc
    65761 ttcaccaaaa gatcgctgat gaaattttgc gcggttaacg ccgcaaaacg cctgtcgaaa
    65821 cgcacgcaca gcacgtaatc aacgccacac tctgcaaggt aacgcagttt ttcccgcagc
    65881 cgggtcagtc ttgccggggc tttatcggta gcaaacagtt ccagtggttg aggttcaaaa
    65941 agcatcacca tcaccggtaa gttgcgcttg cgcccttctt cctgcaagcc ctgtaacagc
    66001 gcgcgatgac cgcgatgcac gccgtcgaaa ttaccaatag tcagcacaca cccttcttgc
    66061 ggggcctggc tgagattatg tatgccgcgt atcagcttca tgtctggctc aaaacagtga
    66121 aaatcgtccg agtatacctt gtacagcggt caaggttaac cggcgattga gtaccgaaat
    66181 cagaaagaag tggcttttca ttgccatgga gcaaatcacg ggaagaaact gaccgcctgc
    66241 tgcaattttt atcgcggaaa agctgtattc acaccccgca agctggtaga atcctgcgcc
    66301 atcactacgt aacgagtgcc ggcacattaa cggcgcttat ttgcacaaat ccattgacaa
    66361 aagaaggcta aaagggcata ttcctcggcc tttgaattgt ccatatagaa cacatttggg
    66421 agttggacct tggctaatat caaatcagct aagaagcgcg ccattcagtc tgaaaaggct
    66481 cgtaagcaca acgcaagccg tcgctctatg atgcgtactt tcatcaagaa agtatacgca
    66541 gctatcgaag ctggcgacaa agctgctgca cagaaagcat ttaacgaaat gcaaccgatc
    66601 gtggaccgtc aggctgctaa aggtctgatc cacaaaaaca aagctgcacg tcataaggct
    66661 aacctgactg cacagatcaa caaactggct taatcgccaa tttgctgaag ctttgtgaaa
    66721 aagcccgcgc aagcgggttt ttttatgcct gctgcttttg tgatgcgtga gagaacacac
    66781 tgccggttgc cagatagtca aaagagacat gccgttgtac tatctgaact cttcgtggct
    66841 taagaatcgt agaaccatcg gctgatacat tctgaacaag aatatctact caatctccag
    66901 actcccccac aaagaatatg gatattgtga tacacattga ggtattaaac ttaagaccac
    66961 tctatttttg aattaaattc ctatatactt taaccattca taaatatctc ctcattagac
    67021 ct
//
LOCUS       DAJTRN010000009        65689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRN010000009 DAJTRN010000000
VERSION     DAJTRN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OO328_000599"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OO328_000599"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5929281.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO328_000600"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO328_000600"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5929282.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO328_000601"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO328_000601"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5929283.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO328_000602"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO328_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5929284.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO328_000603"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO328_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5929285.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO328_000604"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO328_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5929286.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OO328_000605"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OO328_000605"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5929287.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="OO328_000606"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="OO328_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5929288.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="OO328_000607"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="OO328_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5929289.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="OO328_000608"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="OO328_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5929290.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="OO328_000609"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="OO328_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5929291.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="OO328_000610"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="OO328_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5929292.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OO328_000611"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OO328_000611"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5929293.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="OO328_000612"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="OO328_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5929294.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /locus_tag="OO328_000613"
     CDS             19305..19370
                     /locus_tag="OO328_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929295.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="OO328_000614"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="OO328_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5929296.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="OO328_000615"
     CDS             complement(19754..20377)
                     /locus_tag="OO328_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5929297.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OO328_000616"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OO328_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5929298.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OO328_000617"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OO328_000617"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5929299.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="OO328_000618"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="OO328_000618"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5929300.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="OO328_000619"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="OO328_000619"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5929301.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /gene="cdaR"
                     /locus_tag="OO328_000620"
                     /pseudo
     CDS             25216..26372
                     /gene="cdaR"
                     /locus_tag="OO328_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26461..26847)
                     /locus_tag="OO328_000621"
     CDS             complement(26461..26847)
                     /locus_tag="OO328_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5929302.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OO328_000622"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OO328_000622"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5929303.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OO328_000623"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OO328_000623"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5929304.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="OO328_000624"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="OO328_000624"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5929305.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="OO328_000625"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="OO328_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5929306.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="OO328_000626"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="OO328_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5929307.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="OO328_000627"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="OO328_000627"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5929308.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="OO328_000628"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="OO328_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5929309.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="OO328_000629"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="OO328_000629"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5929310.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="OO328_000630"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="OO328_000630"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5929311.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="OO328_000631"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="OO328_000631"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5929312.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="OO328_000632"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="OO328_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5929313.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="OO328_000633"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="OO328_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5929314.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="OO328_000634"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="OO328_000634"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5929315.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="OO328_000635"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="OO328_000635"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5929316.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="OO328_000636"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="OO328_000636"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5929317.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="OO328_000637"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="OO328_000637"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5929318.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="OO328_000638"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="OO328_000638"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5929319.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="OO328_000639"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="OO328_000639"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5929320.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..50646
                     /gene="dnaE"
                     /locus_tag="OO328_000640"
     CDS             47164..50646
                     /gene="dnaE"
                     /locus_tag="OO328_000640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5929321.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50659..51618
                     /gene="accA"
                     /locus_tag="OO328_000641"
     CDS             50659..51618
                     /gene="accA"
                     /locus_tag="OO328_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5929322.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51717..53858
                     /gene="ldcC"
                     /locus_tag="OO328_000642"
     CDS             51717..53858
                     /gene="ldcC"
                     /locus_tag="OO328_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5929323.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53915..54304
                     /locus_tag="OO328_000643"
     CDS             53915..54304
                     /locus_tag="OO328_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5929324.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54369..55667
                     /gene="tilS"
                     /locus_tag="OO328_000644"
     CDS             54369..55667
                     /gene="tilS"
                     /locus_tag="OO328_000644"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5929325.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OO328_000645"
     CDS             complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OO328_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5929326.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55963..56163)
                     /locus_tag="OO328_000646"
     CDS             complement(55963..56163)
                     /locus_tag="OO328_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5929327.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56329..56874
                     /locus_tag="OO328_000647"
     CDS             56329..56874
                     /locus_tag="OO328_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5929328.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56871..57293
                     /gene="arfB"
                     /locus_tag="OO328_000648"
     CDS             56871..57293
                     /gene="arfB"
                     /locus_tag="OO328_000648"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5929329.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57307..58017
                     /gene="nlpE"
                     /locus_tag="OO328_000649"
                     /gene_synonym="cutF"
     CDS             57307..58017
                     /gene="nlpE"
                     /locus_tag="OO328_000649"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5929330.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58172..58996)
                     /locus_tag="OO328_000650"
     CDS             complement(58172..58996)
                     /locus_tag="OO328_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5929331.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OO328_000651"
     CDS             complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OO328_000651"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5929332.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OO328_000652"
     CDS             complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OO328_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5929333.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OO328_000653"
     CDS             complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OO328_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5929334.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OO328_000654"
     CDS             complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OO328_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5929335.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OO328_000655"
     CDS             complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OO328_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5929336.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OO328_000656"
     CDS             complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OO328_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5929337.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64824..65399
                     /gene="gmhB"
                     /locus_tag="OO328_000657"
     CDS             64824..65399
                     /gene="gmhB"
                     /locus_tag="OO328_000657"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5929338.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15532 a        16277 c        18098 g        15782 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccacca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gggatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaaaataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acagcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagcg caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggta aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct
    10321 cgtttacgtt attattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg gcagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accacctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgttcaa tctgtacaat ccggtgaatc ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa
    11881 cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc agcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgatttt cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggttgtta cccgcaggca gtctcggggc ggcggtgacg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcctgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gcttcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac ggcgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattccat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggg gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcagtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggggcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggggatttc gcggaccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgctaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccgtag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atagcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccgcttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca gtcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaacac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcggcgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccgatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagtgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagtccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 acatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtggag agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat
    34441 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgagtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttga cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga tcttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag caactgacta agatcgagcc gggcgagctg tataacggca
    40861 ctaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43381 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg ttattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctggcatt tttattccct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48241 ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg
    49501 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49561 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49621 ctttaccatt tccacgtcaa cgacgatggc gaaatcgtgt atggtattgg cgccatcaaa
    49681 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49741 cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49801 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg
    49861 aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49921 caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc
    49981 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    50041 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    50101 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    50161 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50221 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50281 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50341 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50401 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50461 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50521 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50581 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50641 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50701 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50761 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50821 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat
    50881 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg
    50941 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    51001 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    51061 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51121 atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51181 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg
    51241 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51301 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51361 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51421 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51481 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51541 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51601 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51661 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51721 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51781 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51841 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51901 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51961 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    52021 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca
    52081 acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag
    52141 agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc
    52201 cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct gatgtctcta
    52261 tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacacctg gaagcggaag
    52321 agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac ggaatatcga
    52381 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc
    52441 gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52501 tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt gaatttactc
    52561 gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg gttcatgcgg
    52621 tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc aaacagacgc
    52681 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat tttcatccaa
    52741 tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt ttcttcgaaa
    52801 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc cacattaaag
    52861 gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52921 gttatcccat tgttgcttcg gttaagacgg cggcggcgat gctgcgtggt aatccgggca
    52981 aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggc
    53041 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    53101 ccgaatgcag gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg
    53161 atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg
    53221 gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc gacgaacgtg
    53281 ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg
    53341 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc
    53401 tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc
    53461 gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53521 tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc
    53581 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53641 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag
    53701 gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg
    53761 tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg
    53821 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53881 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53941 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg
    54001 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    54061 gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag
    54121 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54181 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54241 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54301 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54361 gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg
    54421 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54481 aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg
    54541 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54601 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcacgtt
    54661 atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg
    54721 acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54781 cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg ttgcttgccc
    54841 gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54901 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54961 tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccaca ctttgtgctg
    55021 aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc
    55081 aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcgatta
    55141 ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgca
    55201 tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcct
    55261 ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg
    55321 aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa
    55381 ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55441 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga cgtaagctga
    55501 agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55561 tttatggaga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg
    55621 aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat
    55681 aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac caccaccgta
    55741 ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggtttcgcca
    55801 gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact ggcttttgcc
    55861 tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc
    55921 tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat tgacataatc
    55981 gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc
    56041 gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta
    56101 tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc
    56161 cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga
    56221 taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac
    56281 tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa
    56341 gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat
    56401 gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg
    56461 ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat
    56521 gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg
    56581 gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga agtggcgctg
    56641 tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg
    56701 cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc
    56761 tttgccgatc gtaccatgac gctgcaggca acgattcagg atggtgttat ctggttatcg
    56821 gatgataaga ataatctgga agttaactta accgcctggc aacaaccttc atgattgtga
    56881 tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg
    56941 gcgctggcgg gcagcatgtt aataagacct caacggctat tcatctgcgt tttgacattc
    57001 gggcgtccag cctgccagag tattacaaag agcgtctgct cgccgccagc catcatttga
    57061 tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc
    57121 gcgaagccgc tctggcccgg ctggtggcag tgattaaaga tttaacaaaa gaacaaaaag
    57181 cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac
    57241 aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa taaaaagaag
    57301 gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg
    57361 atgggatgta ataatcgggc cgaagtcgat acgctttctc cggcgcaggc tgccgaactg
    57421 aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga aggaatcgaa
    57481 atctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct
    57541 cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg
    57601 gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag
    57661 atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca actatacgct ggaaccggca
    57721 caatccagtt tacctatgac gccgatgacc ctgcgaggca tgtattttta tatggctgat
    57781 gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag
    57841 ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt actgtcagta
    57901 gaaggtcact ttacgcttga ggctaatccg gatactggtg cgccaaccaa agtattagcg
    57961 cccgatacgg cgggtaaatt ttacccgaat aaggattgca gtagtttggg gcagtaaacc
    58021 tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca ggcctacagc
    58081 atataatgca acattttgaa ttagcacgat tttgtaggcc ggataaggcg tttacgccgc
    58141 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
    58201 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58261 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58321 gcgccagtgt aagtggatgc ccggatttgg caaatgcatc agtgacaaat tccgaacaaa
    58381 accacgactt tttgtctcct tcgcacacac tgcttaactg cgctttcgcc aggtcgctga
    58441 cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
    58501 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
    58561 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    58621 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    58681 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    58741 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    58801 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
    58861 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
    58921 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg cggcagtacg
    58981 cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat tcaggaccct
    59041 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
    59101 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
    59161 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
    59221 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
    59281 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
    59341 acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta gttctgctca
    59401 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
    59461 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
    59521 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
    59581 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
    59641 tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
    59701 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
    59761 cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
    59821 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
    59881 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
    59941 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
    60001 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
    60061 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
    60121 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggcc
    60181 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
    60241 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
    60301 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
    60361 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
    60421 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
    60481 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcggct
    60541 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
    60601 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
    60661 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
    60721 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
    60781 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga tttagtttac
    60841 cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc agcgcaaaga
    60901 cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc cagacggcat
    60961 aggttttgcc cgtttcctca cctttacgat aggccgggcg cgggtcctgc gccagtactt
    61021 cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc tgcttttcga
    61081 cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca tagctggcac
    61141 tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact accggcgtac
    61201 catcgaccag atccagactg ccgagcttca gaatcacgct gcctttatgg caaacaacct
    61261 ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag cgtgttgcga
    61321 aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga cgccagccgc
    61381 cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaatgct tccaggccgc
    61441 gaacggcgtc ggcctggttg taggaagcta tgagatgcag ttcgccgttg gcgcttttta
    61501 ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga ataacgccta
    61561 tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc cgatacatac
    61621 agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca gtaatacagc
    61681 attggctttc attttagagg cattgatttg catctgctta cgtgcggttg gaatgctcgg
    61741 cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga gatcgcggaa
    61801 cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg cgcgcggcgc
    61861 tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg gttcgacagg
    61921 ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga tcggtaaagc
    61981 acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg agcttgtaaa
    62041 aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga aacagcctaa
    62101 aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt cgtaaacttc
    62161 atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt tatcttcacg
    62221 cgttacgatc aggtttacgt atggggagtc tttatcttcg acaaagatac cgtctttcgc
    62281 cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag cgatttgcgc
    62341 gtcgtccaga gaacgcggca gttgcggtgc ttccagttca acaattttca gattttttgg
    62401 gttctcaaca acatcaagaa cggtcggcag caggccaacg ccatctttca gtttgatcaa
    62461 gcccactttt tgcagcagca gcagtgaacg accaagatta gttggatcgt ttggcacggc
    62521 aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt aaccagcaat
    62581 cggataaaca aaagtgttgc ctactgcgac cagtttgtag ccacgatctt tcagttgctg
    62641 atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc tcaatgcttc
    62701 gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat atttgtcttt
    62761 cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca cgccgacttt
    62821 aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg atccaatcag
    62881 ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct tcttcaatta
    62941 tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt cgcctgcaaa
    63001 ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca ccgtcgcgtt
    63061 gtagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac cgactgcacc
    63121 acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat tcaccagacc
    63181 cggcaacgct tccggtaaca gcactttaca gacgatctgc attggcgtgg cacccattgc
    63241 gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga ccatacgggc
    63301 aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac caatcgatgt
    63361 accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga acgggatgga
    63421 acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag caataatttg
    63481 ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa acccaaaaaa
    63541 tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca gccacatcat
    63601 cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc caggcaatgg
    63661 cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg aatttaacgc
    63721 caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa cgacgcgcgg
    63781 tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc agcatcggca
    63841 cgcagtcagt aaatggctcc gtttgcagac gttcctggta atcttccggg atatccagat
    63901 gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag aacacttcac
    63961 ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa tcacaaatac
    64021 gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga cggcggttaa
    64081 tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg ctggtggctt
    64141 catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc acacgttgtt
    64201 tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga ccaaccaatg
    64261 acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc agctccagcg
    64321 gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc tggaaaatca
    64381 taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc aattcctggc
    64441 catcgaccag cacgctaccc tcggttgggc gctccagcaa gtttacacaa cgtataagcg
    64501 tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct ggcacatgca
    64561 ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact ttggtgatat
    64621 tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtag catctcgaat
    64681 gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac gtctaaatca
    64741 atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact agcgtcccat
    64801 gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt cttgaccgtg
    64861 atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt gaatttatcg
    64921 acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg gtggtagtaa
    64981 ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa acgctgaccg
    65041 agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc tattattgcc
    65101 cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc cgcaaaccac
    65161 atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc gcttcttata
    65221 tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg ggaacaaaag
    65281 tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca gattgggtgt
    65341 taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg gcgcaatgat
    65401 taaaagatga acggttgaaa taaaaatgca tttttccgct tgtcttcctg agccgactcc
    65461 ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg
    65521 gttgaagaga aaaaatcctg aaattcaggg ttgactctga aataggaaag tgtgatatac
    65581 gccacctcgc gacagtgagc tgaaagctgc gtcgcaactg ctctttaaca atttatcaga
    65641 caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacga
//
LOCUS       DAJTRN010000010        64030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRN010000010 DAJTRN010000000
VERSION     DAJTRN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            132..575
                     /locus_tag="OO328_000658"
     CDS             132..575
                     /locus_tag="OO328_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5929339.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1629
                     /gene="ybhA"
                     /locus_tag="OO328_000659"
     CDS             811..1629
                     /gene="ybhA"
                     /locus_tag="OO328_000659"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5929340.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1630..2688)
                     /gene="modC"
                     /locus_tag="OO328_000660"
     CDS             complement(1630..2688)
                     /gene="modC"
                     /locus_tag="OO328_000660"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5929341.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2691..3380)
                     /gene="modB"
                     /locus_tag="OO328_000661"
     CDS             complement(2691..3380)
                     /gene="modB"
                     /locus_tag="OO328_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5929342.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3380..4153)
                     /gene="modA"
                     /locus_tag="OO328_000662"
     CDS             complement(3380..4153)
                     /gene="modA"
                     /locus_tag="OO328_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5929343.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="OO328_000663"
     CDS             complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="OO328_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5929344.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4598..5386
                     /gene="modE"
                     /locus_tag="OO328_000664"
     CDS             4598..5386
                     /gene="modE"
                     /locus_tag="OO328_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5929345.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5454..6926
                     /gene="modF"
                     /locus_tag="OO328_000665"
     CDS             5454..6926
                     /gene="modF"
                     /locus_tag="OO328_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5929346.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7187..8203
                     /gene="galE"
                     /locus_tag="OO328_000666"
     CDS             7187..8203
                     /gene="galE"
                     /locus_tag="OO328_000666"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5929347.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8213..9259
                     /gene="galT"
                     /locus_tag="OO328_000667"
     CDS             8213..9259
                     /gene="galT"
                     /locus_tag="OO328_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5929348.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9263..10411
                     /gene="galK"
                     /locus_tag="OO328_000668"
     CDS             9263..10411
                     /gene="galK"
                     /locus_tag="OO328_000668"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5929349.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10405..11445
                     /gene="galM"
                     /locus_tag="OO328_000669"
     CDS             10405..11445
                     /gene="galM"
                     /locus_tag="OO328_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5929350.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11648..12400
                     /gene="gpmA"
                     /locus_tag="OO328_000670"
     CDS             11648..12400
                     /gene="gpmA"
                     /locus_tag="OO328_000670"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5929351.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="OO328_000671"
     CDS             complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="OO328_000671"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5929352.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13935..14315
                     /locus_tag="OO328_000672"
     CDS             13935..14315
                     /locus_tag="OO328_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5929353.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14429..15370
                     /gene="zitB"
                     /locus_tag="OO328_000673"
     CDS             14429..15370
                     /gene="zitB"
                     /locus_tag="OO328_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5929354.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="OO328_000674"
     CDS             complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="OO328_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5929355.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="OO328_000675"
     CDS             complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="OO328_000675"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5929356.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17416..17491)
                     /locus_tag="OO328_000676"
     tRNA            complement(17416..17491)
                     /locus_tag="OO328_000676"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17456..17458),aa:Lys,seq:ttt)
     gene            complement(17495..17570)
                     /locus_tag="OO328_000677"
     tRNA            complement(17495..17570)
                     /locus_tag="OO328_000677"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17535..17537),aa:Val,seq:tac)
     gene            complement(17622..17697)
                     /locus_tag="OO328_000678"
     tRNA            complement(17622..17697)
                     /locus_tag="OO328_000678"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17662..17664),aa:Lys,seq:ttt)
     gene            complement(17700..17775)
                     /locus_tag="OO328_000679"
     tRNA            complement(17700..17775)
                     /locus_tag="OO328_000679"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17740..17742),aa:Val,seq:tac)
     gene            complement(17911..17986)
                     /locus_tag="OO328_000680"
     tRNA            complement(17911..17986)
                     /locus_tag="OO328_000680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17951..17953),aa:Lys,seq:ttt)
     gene            complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="OO328_000681"
     CDS             complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="OO328_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5929357.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18952..19473)
                     /gene="pal"
                     /locus_tag="OO328_000682"
     CDS             complement(18952..19473)
                     /gene="pal"
                     /locus_tag="OO328_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5929358.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="OO328_000683"
     CDS             complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="OO328_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5929359.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="OO328_000684"
     CDS             complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="OO328_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5929360.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="OO328_000685"
     CDS             complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="OO328_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5929361.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="OO328_000686"
     CDS             complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="OO328_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5929362.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="OO328_000687"
     CDS             complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="OO328_000687"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5929363.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="OO328_000688"
     CDS             complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="OO328_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5929364.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="OO328_000689"
     CDS             complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="OO328_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5929365.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="OO328_000690"
     CDS             complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="OO328_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5929366.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="OO328_000691"
     CDS             complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="OO328_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5929367.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27907..30540)
                     /gene="mngB"
                     /locus_tag="OO328_000692"
     CDS             complement(27907..30540)
                     /gene="mngB"
                     /locus_tag="OO328_000692"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5929368.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30558..32474)
                     /gene="mngA"
                     /locus_tag="OO328_000693"
     CDS             complement(30558..32474)
                     /gene="mngA"
                     /locus_tag="OO328_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5929369.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32644..33366
                     /gene="mngR"
                     /locus_tag="OO328_000694"
     CDS             32644..33366
                     /gene="mngR"
                     /locus_tag="OO328_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5929370.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33469..34338)
                     /gene="sucD"
                     /locus_tag="OO328_000695"
     CDS             complement(33469..34338)
                     /gene="sucD"
                     /locus_tag="OO328_000695"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5929371.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34338..35504)
                     /gene="sucC"
                     /locus_tag="OO328_000696"
     CDS             complement(34338..35504)
                     /gene="sucC"
                     /locus_tag="OO328_000696"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5929372.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35598..36815)
                     /gene="odhB"
                     /locus_tag="OO328_000697"
     CDS             complement(35598..36815)
                     /gene="odhB"
                     /locus_tag="OO328_000697"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5929373.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36830..39631)
                     /gene="sucA"
                     /locus_tag="OO328_000698"
     CDS             complement(36830..39631)
                     /gene="sucA"
                     /locus_tag="OO328_000698"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5929374.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39932..40648)
                     /gene="sdhB"
                     /locus_tag="OO328_000699"
     CDS             complement(39932..40648)
                     /gene="sdhB"
                     /locus_tag="OO328_000699"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5929375.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40664..42430)
                     /gene="sdhA"
                     /locus_tag="OO328_000700"
     CDS             complement(40664..42430)
                     /gene="sdhA"
                     /locus_tag="OO328_000700"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5929376.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42430..42777)
                     /gene="sdhD"
                     /locus_tag="OO328_000701"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42430..42777)
                     /gene="sdhD"
                     /locus_tag="OO328_000701"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5929377.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42771..43175)
                     /gene="sdhC"
                     /locus_tag="OO328_000702"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42771..43175)
                     /gene="sdhC"
                     /locus_tag="OO328_000702"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5929378.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43556..43663
                     /locus_tag="OO328_000703"
     CDS             43556..43663
                     /locus_tag="OO328_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929379.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43869..45152
                     /gene="gltA"
                     /locus_tag="OO328_000704"
     CDS             43869..45152
                     /gene="gltA"
                     /locus_tag="OO328_000704"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5929380.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45543..46109
                     /locus_tag="OO328_000705"
     CDS             45543..46109
                     /locus_tag="OO328_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5929381.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46160..48619
                     /locus_tag="OO328_000706"
     CDS             46160..48619
                     /locus_tag="OO328_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5929382.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48634..49365
                     /locus_tag="OO328_000707"
     CDS             48634..49365
                     /locus_tag="OO328_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5929383.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49362..50438
                     /locus_tag="OO328_000708"
                     /pseudo
     CDS             49362..50438
                     /locus_tag="OO328_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50590..51636
                     /locus_tag="OO328_000709"
     CDS             50590..51636
                     /locus_tag="OO328_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5929384.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51633..52424)
                     /gene="nei"
                     /locus_tag="OO328_000710"
     CDS             complement(51633..52424)
                     /gene="nei"
                     /locus_tag="OO328_000710"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5929385.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52460..53194)
                     /gene="pxpA"
                     /locus_tag="OO328_000711"
     CDS             complement(52460..53194)
                     /gene="pxpA"
                     /locus_tag="OO328_000711"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5929386.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53184..54116)
                     /gene="pxpC"
                     /locus_tag="OO328_000712"
                     /pseudo
     CDS             complement(53184..54116)
                     /gene="pxpC"
                     /locus_tag="OO328_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54110..54766)
                     /gene="pxpB"
                     /locus_tag="OO328_000713"
     CDS             complement(54110..54766)
                     /gene="pxpB"
                     /locus_tag="OO328_000713"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5929387.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54789..55532)
                     /gene="ybgI"
                     /locus_tag="OO328_000714"
     CDS             complement(54789..55532)
                     /gene="ybgI"
                     /locus_tag="OO328_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5929388.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55803..57284
                     /gene="dtpD"
                     /locus_tag="OO328_000715"
     CDS             55803..57284
                     /gene="dtpD"
                     /locus_tag="OO328_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5929389.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57434..58852)
                     /gene="phrB"
                     /locus_tag="OO328_000716"
     CDS             complement(57434..58852)
                     /gene="phrB"
                     /locus_tag="OO328_000716"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5929390.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58849..59358)
                     /locus_tag="OO328_000717"
     CDS             complement(58849..59358)
                     /locus_tag="OO328_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5929391.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59487..59774
                     /locus_tag="OO328_000718"
     CDS             59487..59774
                     /locus_tag="OO328_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5929392.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59829..60209)
                     /locus_tag="OO328_000719"
     CDS             complement(59829..60209)
                     /locus_tag="OO328_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929393.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60212..63598)
                     /locus_tag="OO328_000720"
     CDS             complement(60212..63598)
                     /locus_tag="OO328_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5929394.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63617..63820)
                     /locus_tag="OO328_000721"
                     /pseudo
     CDS             complement(63617..63820)
                     /locus_tag="OO328_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15628 a        17244 c        15603 g        15555 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg caaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaattgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcaaaat
      601 gccacttgta attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat
     1021 ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc
     1081 ctgcaactca ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgat
     1141 gcaatggtct atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc
     1201 ctgccgccgg aacagcgtcc gactttcaca caagtcgctt ctctggctga aacgacgcaa
     1261 caagttaacg ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat
     1321 tttggtaagc atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag
     1381 gttgatattg cacacggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg
     1441 caaggttggt cgatggaaaa cgtcgtggca ttcggcgata actttaatga tatcagtatg
     1501 ctggaagccg ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc
     1561 gccaacattg tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac
     1621 ctgatttaat caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg
     1681 tttgatcgcc agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc
     1741 gacttccagc tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg
     1801 caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc
     1861 ctgaatgcgg atacgtagcg cagcttgcag cggttcgccc agcttattga cccacaaatg
     1921 ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gcaccgtcac
     1981 tttcagaatg ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca
     2041 cacttcctcc agagcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc
     2101 tgccagatgg aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc
     2161 ccgtgtcagc cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc
     2221 cagcggttca tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac
     2281 gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc
     2341 taaaagcgcc accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag
     2401 attgccacgc actttgtaat gcgggaacaa ccgcgcatcc tgaaaaacat agccaacacg
     2461 acgcttttcc ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag
     2521 gacaatccgc cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt
     2581 tccggcaccg gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat
     2641 ggtcaggcaa tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc
     2701 gcccgttcac ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc
     2761 gcaatagaaa taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg
     2821 gtatacatgg cagaaggaat ggttcgcgtt tcgctaggaa tgttcgacac aaaggtgatg
     2881 gttgcaccaa actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc
     2941 ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt
     3001 cttgcggcct gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc
     3061 atcagcggaa acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag
     3121 gtaataccaa accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact
     3181 aataataagt aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg
     3241 agcagagctt tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac
     3301 ggcaggctaa acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat
     3361 tctggatcgg tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg
     3421 cctgcggtcc cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca
     3481 caacagcaac cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt
     3541 ttaccccttt gctggcaact gcgtcagaac cgtagacaat gcccagaggc gcttcgttac
     3601 gttcgaccag cgccagcgcc ccacgaacat cttccgctgg ggccagtttc ggagagagcg
     3661 tatcccatgc gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt
     3721 ccggatcgcc aaccgccagg cgaccgccat tcagcagcga agtccagtta gttttgctgt
     3781 cgatggtgaa atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga
     3841 gcagtgtctg acgcgtagca gtatcgatcg cttttttatc aaccgcataa tccatccatt
     3901 tctgatcggc agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag
     3961 tagatgacga agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag
     4021 caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat
     4081 ctgccagtgc attgccagca actgcgaaag agagtgccgc cccggcaaac aagttcaacc
     4141 atttacgagc cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta
     4201 actcggaatt ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg
     4261 gcaggaaaag caggaagttg agaaaaagaa aatgcccgac taagcgggca ctcagggaat
     4321 caatgatttt gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca
     4381 ccaagaccgt aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg
     4441 aataccagac tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt
     4501 ctaaccgcta agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg
     4561 ttttccgtca caataagact tttgccagga cattgttatg caggccgaaa tccttctcac
     4621 ccttaagctc caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat
     4681 tgcgctttcc ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc
     4741 ctgggatgcc attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac
     4801 aggcggtaaa ggtggcggag gcgcagtact gacccgctat ggtcagcgac tgattcagct
     4861 ctatgactta ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc
     4921 cctgccgctg aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg
     4981 taaccagtgg ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga
     5041 tgtcttactg gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc
     5101 acgtctgggg ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg
     5161 cattactcag gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag
     5221 tcatattgag cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac
     5281 actgtgcgcc acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac
     5341 ggcctacttt aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt
     5401 tgttatgaaa cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat
     5461 cgttgcaaat tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc
     5521 agctaacgtt aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agtggaaagt
     5581 cggcgctggc ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc
     5641 agttttccca catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat
     5701 ggcaacggaa taacaccgat atgctcggcc ctagcgaaga ttacaccgga cgcactacgg
     5761 ctgagattat tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt
     5821 tcggtattac cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa
     5881 aaaccctgct gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt
     5941 tcgatggcct ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc
     6001 agtccggtat tactttggta ctggtgctca atcgcttcga taagatcccg gaatttgtcc
     6061 agtttgctgg cgtgctggcg gattgtacgt tagcggaaac tggcgctaaa gaggaactgc
     6121 tccagcaagc actcgtcgcg caacttgcgc atagtgaaca gcttgaaggt gtgcaactgc
     6181 cggagccgga tgaaccttca gcacgtcacg ccttacccgc caacgaatcg cgcattgtgc
     6241 tgaacaatgg cgtggtttct tataatgatc gccccattct taataacctt agctggcagg
     6301 tgaatccagg cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat
     6361 taagcctgat tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggcc
     6421 gacgtcgcgg cagcagcgaa accatctggg acatcaaaaa gcatatcggt tacgtcagca
     6481 gtagtttgca tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct
     6541 attttgattc gattggcatt tatcaggtcg tttcggatcg ccagcaaaaa ctggtgcagc
     6601 agtggctgga tattctcggc attgataaac acacggctga cgctccgttc catagtcttt
     6661 cctggggaca gcagcgtttg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc
     6721 ttattctcga tgaaccacta caggggcttg atccgctcaa tcgccaactt atccgccgtt
     6781 ttgttgatgt gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg
     6841 aagatgcgcc tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc
     6901 gctatgtgct gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcggcctaa
     6961 gcgcactctc cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat
     7021 aaaaagcgca atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac
     7081 ggctaaattc ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt
     7141 tgttatgcta tggttatttc ataccataag cctaatggag cgaattatga gagttctggt
     7201 taccggtggt agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca
     7261 tgatgtcatc attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga
     7321 gcgtttaggc ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat
     7381 gaccgagatc ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc
     7441 cgtcgccgaa tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct
     7501 gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc
     7561 caccgtttat ggcgatcagc cccaaattcc atacgttgaa agcttcccga ccggcacacc
     7621 gcaaagccct tatggcaaaa gcaaattgat ggtggaacag atcctcaccg acctgcaaaa
     7681 agcccagccg gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc
     7741 gtcgggcgat atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc
     7801 ccaagttgct gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga
     7861 agatggtact ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt
     7921 ggcgatggaa aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt
     7981 aggcagcagc gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa
     8041 ctatcatttt gcaccgcgtc gcgaaggcga ccttccggcc tactgggcgg acgccagcaa
     8101 agccgaccgt gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac
     8161 ctggcactgg cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca
     8221 atttaatccc gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct
     8281 ggtttcaccg caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca
     8341 ggtgttacct gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg
     8401 cgataaaaac cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat
     8461 gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg
     8521 cggtaccagc cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag
     8581 cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa
     8641 aacgtacccg tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc
     8701 gcatccgcac ggacagattt gggcaaatag cttcctgcct aacgaagctg agtgcgaaga
     8761 ccgcctgcaa aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca
     8821 gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt
     8881 gccttactgg gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttgcg
     8941 gatcaccgat ttgaccgacg cccagcgcag cgatttggcg ctggcgttga aaaagctgac
     9001 cagtcgttat gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc
     9061 accatttaat ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct
     9121 gctgcgctcc gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca
     9181 gcgagacctg accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt
     9241 tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa
     9301 cgcatttggc taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg
     9361 tgaacacacc gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt
     9421 gatcagctgt gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa
     9481 tcagctcgac gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc
     9541 gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt
     9601 ggacatggtg atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact
     9661 ggaagtcgcg gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca
     9721 aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat
     9781 ggatcagcta atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgccgctc
     9841 actggggact aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa
     9901 cttcaaacgt accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg
     9961 tgcgcgtttc ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaacgctgt
    10021 tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc
    10081 ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt
    10141 gatggccgag tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga
    10201 cactctggta gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg
    10261 cggcggattt ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca
    10321 gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg
    10381 taaaccatca caaggagcag gacagtgctg aacgaaactc ccgcactggc acccgatggt
    10441 cagccgtacc gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac
    10501 tggggtgcga ctttactttc cgcccgtatt ccgctttccg atggcagcgt ccgcgaggcg
    10561 ctgctcggct gtgccagtcc ggaatgctat caggatcagg ctgcgtttct gggggcctct
    10621 attggtcgtt atgccaaccg catcgccaat agtcgctata cctttgacgg tgaaaccgtg
    10681 acgctttcgc caagccaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa
    10741 cgtcgctggc agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat
    10801 gatggcgatc agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac
    10861 gataaccgta tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg
    10921 actaatcacg tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg
    10981 cagattctgg cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg
    11041 aaatctgtcg ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt
    11101 cttgccgacg acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc
    11161 aaaggcgatg gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg
    11221 aaggtctaca ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca
    11281 ccgtcgcggg gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgagttt
    11341 ctgccggaca gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc
    11401 gaagagtatt ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc
    11461 atgagggctt tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc
    11521 aatattcaca acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg
    11581 ctgttgcttc gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag
    11641 taagcatatg gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa
    11701 agaaaaccgt ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc
    11761 aaaagcagca ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc
    11821 tgtgctgaaa cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg
    11881 gctgcccgtt gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct
    11941 gaacaaagcg gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg
    12001 ttttgcagtg actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc
    12061 gcgttacgcg aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat
    12121 tgaccgcgtg atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg
    12181 cgtaatcatc gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat
    12241 gagcgaagaa gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt
    12301 cgacgagaat ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc
    12361 gaaagcagca gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat
    12421 gagaaagccg actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca
    12481 ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact
    12541 ctgacggcgc atccgacaat taaaccttac ccgcgacgcg cttttactgc attcaccagt
    12601 tgacgtaaca gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg
    12661 taggccagcg gctccccgct atcgaggctc tgattgcctt ccaccagatg gctttccacc
    12721 atcacgccaa taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca
    12781 tccatctgct ttttgaattg tttggacgaa ttagcatggc tgaaatcgat catcacctga
    12841 gctggcagac ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag
    12901 ttaggctctt taccgccgcg cagaatgata tggcaatcgc cgttaccact ggtattcaca
    12961 atcgccgaat gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta
    13021 atggcatcga tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa
    13081 gaaagaccag acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg
    13141 ccccagctca tcaggtcagc gagatactga ggagtgatca tatcgagaaa ctcacccgcc
    13201 gctggcagac cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg
    13261 ttgatctgga agctgttatc catatgcgga tcgttaatca accctttcca gcccaccgtg
    13321 gtacgcggct tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca
    13381 cgcagcgcca gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac
    13441 gggccaatca ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt
    13501 cgggcatggg caaccgtatt cgcggcattt tcagttgcgg ggaatttttc cagcaatgcg
    13561 acaggaggaa gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg
    13621 tctgttccag tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc
    13681 gtcagaatgt gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa
    13741 aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt
    13801 gtttgttaat tatttcaaga ttctctgctg cgtttcataa actggctgaa atcttaaacc
    13861 aatgccttat attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat
    13921 aacaggagca tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa
    13981 gccttgccag cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg
    14041 caaacgccgc agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa
    14101 ataacgtcga caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg
    14161 gttcttcaat gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca
    14221 aagatggtcg atgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg
    14281 atgtcgatac caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga
    14341 gagcggagtc tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta
    14401 agtgagaata attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca
    14461 cctgccagaa gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt
    14521 tatgctggta gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc
    14581 gggtcatatg ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc
    14641 acgtcgtcct cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc
    14701 gtttgtgaat gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga
    14761 acgcttccgc acgccgcgtc cggtcgaggg aggcatgatg atggcaattg ccgtggctgg
    14821 gttgctggca aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct
    14881 caacgtacga gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat
    14941 tattgccgcg ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat
    15001 actggtgtcg cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga
    15061 attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg
    15121 ggaaatcccg gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc
    15181 ggtgatgacg ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca
    15241 gatccaacac tacctgatgg atcactatca gattgagcac accaccattc agatggaata
    15301 tcaaccttgt cataggccag actgccacct gaatgaaggg gtgtctggcc attcacatca
    15361 tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg
    15421 gctgccgttg agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc
    15481 ctgaagtgca aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc
    15541 cacatactta cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc
    15601 ccagaacggg aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg
    15661 catgatcatg actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat
    15721 cagaccaatc gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca
    15781 gcgaattttc aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta
    15841 aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa
    15901 gaaaataatg ccaaacaagg taacgttgat caggccaaag aagtagttgc tgatcttctc
    15961 cagactggca aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga
    16021 gagatcataa ccgcccgccc ctattggtat atgtaccagg atattctgca cactaaaaaa
    16081 atccatcttt cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag
    16141 ccgcaaaatc cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctctcatcga
    16201 catgaacctc gtgattactt ccttcctgtt ctaatgcctc tgcgatagcc tgaaggtcat
    16261 tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg
    16321 gtgcttccag taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat
    16381 cggttgccac aataagcctc tgatgaggca atgcttttgc agcagcgatc agttgactgg
    16441 tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta
    16501 tggcagcatc cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat
    16561 gcacaatgca ggcaccctgc cagcataaaa tgtctccacc cgtctgtttt tgcacgtaac
    16621 gccccagatg tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa
    16681 taagttcgac ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag
    16741 aagtgttggc gtagacgacg acagtacgat cgggatgggc atcgtaaaat gcgttaaatt
    16801 cttcaacagg gcagccgaga tccagtgaac actcaacctg aagtgtcggc atcagaattg
    16861 ttttttccgg actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca
    16921 aagtagaagc gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc
    16981 caccggtttc ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca
    17041 ccgcattacg ttcttttagc agacgtttta tcttctcgcg gtaatacgct ttttcatcaa
    17101 tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta
    17161 cgctcatctt accatctcgt tttacaggct taacgttaaa accgacatta gcgtaacatt
    17221 cgctgttatc ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa
    17281 gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg
    17341 atgtcatcag gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag
    17401 ggcgcttttt tgcattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    17461 tcaactgctc taccaactga gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg
    17521 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactgcgc
    17581 tggatactac aaactacttc caccgaaccc accgtaatta gtggtgggtc gtgcaggatt
    17641 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccggt
    17701 ggtgggtgat gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag
    17761 ctgagctaat cacccgacac tacgctggat actacaacta actatgttag tgggggtcgt
    17821 gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc
    17881 acgttttact accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga
    17941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
    18001 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
    18061 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
    18121 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
    18181 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
    18241 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
    18301 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
    18361 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
    18421 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
    18481 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
    18541 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg
    18601 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
    18661 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
    18721 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
    18781 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
    18841 gctgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
    18901 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
    18961 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
    19021 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
    19081 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
    19141 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
    19201 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
    19261 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat
    19321 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
    19381 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
    19441 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
    19501 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
    19561 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
    19621 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
    19681 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
    19741 caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcctggt
    19801 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
    19861 cctgcggacg accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg
    19921 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
    19981 ccattacgta caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
    20041 ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
    20101 catttgccag cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca
    20161 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga
    20221 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
    20281 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
    20341 caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt
    20401 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
    20461 cattgtaaga accatccgga ttcggagtga cttgaccgac aactacagcg tcaatgccca
    20521 gtgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
    20581 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa
    20641 tatcttcagg tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac
    20701 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
    20761 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
    20821 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
    20881 ccccgtggca accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt
    20941 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
    21001 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
    21061 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
    21121 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
    21181 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg
    21241 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
    21301 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
    21361 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
    21421 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg
    21481 ctgccgcttt cttatcagct gcagcctttt cagcagccgc tttcttctca gcttctgcct
    21541 tggctttttc agcagcttca gttgccgctt tctttcttgc ttcagctgca gctgcttctg
    21601 ccgcttccgc tttcttcttc agtgccgcag cggctacctc ggcttttttc tgcgcttcgg
    21661 ctgcggcttt ggcggcttct gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg
    21721 ctgcttcttc cgcagcttta gcatctgccg ccgctttcgc tgccgccact tcagcttgct
    21781 tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgc tcctgagccg
    21841 ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca
    21901 gttcttcagc agcctgctgt tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg
    21961 attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag
    22021 cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac
    22081 tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct
    22141 tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct
    22201 gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa
    22261 ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa
    22321 ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg
    22381 gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc
    22441 acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc
    22501 accgcctgtg attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc
    22561 gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg aacaatgttg
    22621 atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg
    22681 ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag
    22741 ttgtcgtaat tcagttccag tttgtttacg cgctggttga gacggttata agccattacc
    22801 gccggaattg cggcgaacag accaattgcc gtcgcaatca acgcttctgc gataccgggc
    22861 gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc
    22921 ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac
    22981 ggaatgtgcg tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac
    23041 gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct
    23101 ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt cccctgactc
    23161 tcttgataga ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct
    23221 tcacgcgccg ctgcgttaag aatacgggtt cgctggataa taatggccca ggatgcgatt
    23281 gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa
    23341 tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac
    23401 gaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag
    23461 tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta
    23521 tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca
    23581 ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat
    23641 gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca
    23701 caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat
    23761 tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc
    23821 gcgcgattga ggtttgggaa tgagggcaag ttaagggcgc gtaaaaattt atgggcctag
    23881 agcggcccat aattttttaa agagaattct gatttaaaag aagaagaaaa tcagcccgac
    23941 aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg
    24001 acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg
    24061 ccagatttcc agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc
    24121 tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc
    24181 atacaaggtc gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg
    24241 acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga
    24301 atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt
    24361 caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca
    24421 gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg
    24481 aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg
    24541 ggaacattgc gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa
    24601 acgcccacgc ggctttatcc atacgtgcag tcaggatggt cagcagcagc agaaccacac
    24661 ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag
    24721 cttcacgaac cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt
    24781 tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca
    24841 gtgtcaccag cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg
    24901 tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg
    24961 ccagcaggcc gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat
    25021 attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca
    25081 gcggcggaac gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt
    25141 cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga
    25201 ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa
    25261 agagtgcgcc gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg
    25321 agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca
    25381 cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc
    25441 accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg
    25501 gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc
    25561 cgaggcgtgc aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc
    25621 cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgaa ttcgccacag
    25681 ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc
    25741 ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt
    25801 ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagca
    25861 gcaggaagcc acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga
    25921 tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat
    25981 agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa
    26041 ccgtctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt
    26101 tacgaatgcg ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca
    26161 cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct
    26221 cctcttcctg atcaggaatg ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc
    26281 actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt
    26341 catcacccag aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa
    26401 aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga
    26461 ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag
    26521 caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt
    26581 tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg
    26641 acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac
    26701 gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac
    26761 cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt
    26821 tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac
    26881 caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt
    26941 agaccgtttc cataatggcc aggaggaacg ccataccgag cgtcagtggc acaaaaagga
    27001 agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca
    27061 tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg
    27121 catgccccaa tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa
    27181 cggcgtaaag aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat
    27241 tacgccgcaa aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
    27301 ggtgatcaat ttatagctaa attgccgcct ttcagccaat ttgatcgaga acaatttatc
    27361 tctttttgat gcccatttcc aagacttata cattgataaa tatcaatttc tacaaattat
    27421 gttaatcatg tttcaacaag gtaactttag tgatatttat gtttaaaatg tgtgaattat
    27481 aagactgaaa ttagtttcgg ttggcaatta aagataaaaa gagacgaagc aaaaacagca
    27541 cggttgacct taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattat
    27601 taataaaggc ataaatctcc ctcccgccga tattaacatt taattaactt ataattatat
    27661 tataattaac tcacccgtgg ttattatttt gttaaaaata aagccacata tacgtagctt
    27721 gagagtgaag ataaacacaa aattacctga tgcgctacgc ttgccagtgt ctacatcatc
    27781 tctgcaatat attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc
    27841 ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat
    27901 tgctattcag gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga
    27961 tagatttgaa ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca
    28021 agaaatcacc tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt
    28081 aaacagccgt aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca
    28141 tcccactggg ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt
    28201 gagcttcatc gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg
    28261 cgcctgctga gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg
    28321 acaagaaagc agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg
    28381 cctaggcctt aaaagcagat cttctttccc cagtaagccc acgccgcgca gcaacgtaat
    28441 ggcaaaggtt ttcttctctt caccgatgac ttcaaattca cgtagccctt cgctaaagac
    28501 agccatgccg ttacgccctt cctgtaaggc tgcatagttg agcatattcc ataccggaac
    28561 cggcgcttct ttccagcttt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg
    28621 cgttagcgaa ccaaactggg tatctgccag aacactatcg gtgttaaaag atgtagggat
    28681 caggacgcga aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg
    28741 cctgttgtta tgactaagag tgacaaccaa ctcgacatct actctgccag tggattgtct
    28801 tgcgctgcgt tgttgcagat tacgcggcac cgccatttca tagcggataa cagccctgct
    28861 ctgccaggcc tcatgaataa tctcgcattg cggtttcgca ttcgccgaag taattaccca
    28921 ctcttctttt gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg
    28981 caacacccgg tcatagcgca caccgctatc tttatctacc agttgtagcg ttccatcctc
    29041 attgagcgca atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc
    29101 aatcacgttg ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac
    29161 aatctggttg atctgtatat caaactccat aaagggatcg taattaccgt aatgaactat
    29221 ttgccgatcg attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg
    29281 ctgaccgcga tcgtctcgta gattaaactg gctggcgcgc agccgcacag tggtgttgat
    29341 cacttcttcc cgcggccagg gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg
    29401 cggcatgttg tcggcgattt tacgcatata gaaacgcagc agattatccg ccatgtcttc
    29461 agccagttcg aagcgggcga cgatttcgcg atgaactttg tcacgacagc agcagccgat
    29521 gctgtcgtgg gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg
    29581 gtatccaaaa cccaacgtcc tggccagcgt tgccagcggt tccagcagat taacaatctt
    29641 attttcaata cgtgcgtggg caactttgat atccatgcgc gtggaaccga tggtgcgatg
    29701 cacgcgcata tatttgccat caataaattc ccctttcagg gttgccagat tatctcgctg
    29761 cgcttcgatc ttctcaaata cctcttcaaa gcggctcatc acaaactttc gttgaggata
    29821 aatttcgcgc agtttctcca tcacttcgaa gatattttgc tgcaatggca tctgatcatg
    29881 cccattcggc agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact
    29941 gtcgaggcgt ttgcgtaatc cgttttcgtc cgcaggtaag tacttaccga tggcataacc
    30001 caacggcatc acctgcgccg tcacttcgct accgtcactg ctttgccaca aaaactcggt
    30061 tttatccgta ccgtggcgct cagaacatcc acgccagaac atggtgcagg taatgccaaa
    30121 tccattgtag atatgcggca gttgcccgga catgccaaag gaatcgggta aataacctat
    30181 tttcatcggc tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat
    30241 ggattccgca gaaacaatcg tggtatcggt ctgggtatac caggggccga taatcaactt
    30301 gccggcttct accagtttct tcaagcggtc tttgttttcc ggtttcaccg cgaaataatc
    30361 ttcgaggatc gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt cctgttccag
    30421 tcggcacagg atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa
    30481 ataccactcc cgatcccagt gcatatgcgg ggtgatgtga acgcgagata ccgctttcat
    30541 cgtcgtttcc tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc
    30601 gcgtgacgcc gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca
    30661 ccaaaccagc caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc
    30721 gagaaaatgc cagcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc
    30781 gttaccatag agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc
    30841 gggatcgccc cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca
    30901 atttcaaact ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc
    30961 gaaaccattt tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg
    31021 cccaggcaga atgcataagc ggctttattc actggccctc caaggtcaaa ggaacacata
    31081 aaaccgagaa tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg
    31141 gtaagcgagt tattgatcca ggcgacaggt tcccccacca caaacagcat cagactgccc
    31201 gctcccaatg taccgagcac cgggtagaga taaaaagtca ggaagccatt gaatttactg
    31261 ctaagacgca agtgattttt catccagcgt atcaagtaac cggctatcaa tccgccaacg
    31321 accgcgccga gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc
    31381 gctaacgccg gtttatctgc cagcgaatag gcggtatagg ctgccagcac cggtaccatc
    31441 aaaattccga gcagcccgcc gcccagtttg cggtacatcc acagccagga gttttcttca
    31501 ttaaacagat cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtc
    31561 ccccccgcga caatcaacgg tacggcaaaa gagatcccgc tcaacagtgc ctgtttcagc
    31621 tccgttttga cgcttttcac cggttgcgta tcttgctgta aggtacgcgt ctcatcgcca
    31681 cgctcgaggg taagcgattg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca
    31741 ggcacagaaa gcgcaggaat gccgttaaaa cgctcactct ccttgatggc gacttcagcc
    31801 gcaaaaatac aggcagttgc actattgagt tgatccgccg ttaaacgccc ttcaataccg
    31861 ttagcacctt gcttttcaac gtagacattc acgccgagtt tgcgtccggc tttttccaga
    31921 tactccgcag ccatgtaggt gtgagcaata cccgccgggc aggccgttac gcaaacgata
    31981 gttggcgcgt tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag
    32041 agcaattcat caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgccagg
    32101 cgcgtggtca gcgctgtcag cagttgcatg tgcgtagtac ccgcttcatt gaggggaatc
    32161 gccagcaaca ccactaaatc aaccgcttcc gggccatcca cgccttccca ctgaagcggc
    32221 tcgctgagtg tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc
    32281 acagccaacc cttcacttaa ggcggtcggg ccaaggcttt cacggcgata cacttctttc
    32341 agaaattgct cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtga
    32401 atggcctctt cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga
    32461 gtcgtcaggt tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga
    32521 caaaacaata ccattgcgta atcaatttca cttgattgaa attatttgca ttttatttgt
    32581 attgtttaca ggatgagttt atcgggcata atggctaaat tcgtattaat gagatgctaa
    32641 ataatgggac acaaaccctt ataccgacag attgctgatc gcattcgtga gcaaattgcg
    32701 cgtggcgagc ttaaacctgg cgatgcgtta cctaccgaat cggcattaca aacagagttt
    32761 ggcgtcagcc gggttacggt gcgtcaggct ttgcgccagt tagtcgagca acagatcctc
    32821 gaaagcattc agggcagcgg gacttacgtc aaagaagagc gggtcaatta cgatattttt
    32881 cagttaacca gttttgatga aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtt
    32941 ctgatatttg aagtgattcc ggctgacgat tttcttcagc aacagctaca aatcaccgtg
    33001 caggatcgtg tttggcatgt gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg
    33061 gaagaaacct ggatgccgct tgcgttgttc ccggatctca cctggcaggt catggaaaat
    33121 tcgaaatatc actttattga agaagtgaag aagatggtta tcgatcgtag cgaacaggaa
    33181 atcattcctc tgatgccaac cgaagagatg agccgtctgc ttaatatcag ccagacaaag
    33241 ccgattcttg aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc
    33301 cgcaacgctt ttaataccga tgactataaa tttaccctga tagcgcagcg aaaatcatcg
    33361 cgataagcac aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg
    33421 ccattcctgt agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac
    33481 agttttcagt gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc
    33541 cagagcagcg aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg
    33601 gcccatacgt ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt
    33661 aacatgctct ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat
    33721 catcacgatc gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt
    33781 agagccaggg atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc
    33841 cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt
    33901 acccggtttg tgaatgtgac ccggctggat accaattttg cattcacccg gggtgataac
    33961 gcctgggcag ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt
    34021 cagcatatcc agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc
    34081 gatagcttcc agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga
    34141 agcgccagta gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt
    34201 agtgccgcct ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg
    34261 ttctgagtgg aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt
    34321 atcaattaaa atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc
    34381 gtcagacctt ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt
    34441 tcggcgttgt taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc
    34501 gcgccgatga taccgtcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc
    34561 agaacggctt tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct
    34621 ttggttgcgc cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact
    34681 atgtccatcg tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc
    34741 agcgcaacgt agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac
    34801 tggtcacgca tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt
    34861 ttgccgtcga ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc
    34921 agcgccaggt cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc
    34981 tgaaccagtt taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc
    35041 gggccagtca gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact
    35101 ttttcgattt ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg
    35161 tctacaacgg caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc
    35221 tggttaaccg gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag
    35281 ttttctgcaa aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta
    35341 ccgcggccac cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa
    35401 gcggcttctt ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca
    35461 tagcgggcaa acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct
    35521 tcagtaatcg ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc
    35581 aggtgaagct taaactacta cacgtccagc agcagacgcg tcggctcttc cagtaactct
    35641 ttgatcgtta ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac
    35701 agcgccaggt acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct
    35761 ttgatagcgt gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc
    35821 agggaaccga acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc
    35881 gtcagcttgc catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc
    35941 atgccgaggg tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc
    36001 gccatgctga cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc
    36061 acttccgggt aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc
    36121 agacggatgc cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc
    36181 ggcttcatgt tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc
    36241 agacgctctg ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca
    36301 gccagagccg gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct
    36361 ttcgccagat gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta
    36421 atggcgctgg cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca
    36481 tcgttgtttt gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct
    36541 tcagatttgg cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc
    36601 tgacgagacg ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct
    36661 gatgccggta cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt
    36721 acgactgcgt cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca
    36781 ggcaggtcag ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt
    36841 tcagcgcgtc attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg
    36901 ccggagaggc ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt
    36961 cacggaaatg atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc
    37021 agacaaaatc cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga
    37081 acgggtagag ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct
    37141 gttccagcag gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg
    37201 gatcaagctc gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga
    37261 ggctggaaac cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac
    37321 gacgcatccc gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg
    37381 gtacgcaaac ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg
    37441 aggagtgctc cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca
    37501 tccggcccca tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt
    37561 tggcgaagtc accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat
    37621 aaccatattc aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg
    37681 cgccctgacc gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt
    37741 ggatcaccgc gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa
    37801 ccggaatgcc ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga
    37861 acagtttctc accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact
    37921 gcatttcaac tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca
    37981 tctcaacttt gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga
    38041 aagagtgcat gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat
    38101 cgcggtacag gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca
    38161 gcttgtcagc gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg
    38221 gctgggttgc gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt
    38281 cgatgaagac atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg
    38341 caacggcttc cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga
    38401 tatcagtaca gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct
    38461 ggttgttgat aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt
    38521 tcagagtttc ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg
    38581 gcagcacttt gttgctgttc ggctcatcaa gtctgtccag acgggcacga acagagccga
    38641 taacgaccgg gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca
    38701 ggccgccatc ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc
    38761 cgaggtgttc tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca
    38821 gcacgttcac cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg
    38881 tgccgctgtt gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc
    38941 cttccagcga gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt
    39001 cagcggcggt cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac
    39061 cagactcgat acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact
    39121 cggcaccaat cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca
    39181 tggtttcttt gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg
    39241 tcagatcgtg gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg
    39301 gatctagatt cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt
    39361 taacctgctt cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt
    39421 tcgccaggcg gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg
    39481 taccaggtaa ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta
    39541 agaagtcttc atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt
    39601 ccaaccaggc tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct
    39661 tacttcgccg tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct
    39721 cttgtctacg tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt
    39781 ttttaaagat ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg
    39841 ggtaatgact tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg
    39901 caacgcttgc gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact
    39961 tgatatggcc gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca
    40021 tgatgctgtg acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag
    40081 tctcggtatc acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga
    40141 taaacttatc gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa
    40201 tacattcata cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag
    40261 ctggcggatt ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga
    40321 attgtcccat gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca
    40381 caatcttctt gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct
    40441 tgccgttcat gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg
    40501 acaggctggg atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac
    40561 cttcttccgc ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat
    40621 agcgataaat tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct
    40681 tcggcgggaa tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt
    40741 ccgactcagg cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt
    40801 cgaagcggct atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat
    40861 acgccgtttc catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc
    40921 tggaagtgtc atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa
    40981 gccctttcgc catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt
    41041 gcagcgcttt acggatcgcc accggatctt caccgttacg attattgttc caccggttca
    41101 ggcgatccag agacgcttca acatcagact cgctggcatc gtgcagtgcg ccctgctcgg
    41161 cgatagactc ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg
    41221 agttgccgcc cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa
    41281 acagtcccgg aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa
    41341 ctttggtcgg aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt
    41401 tcaccggatc gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt
    41461 cgagaacttc tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat
    41521 cacagccgcg accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg
    41581 ccaggtcttt ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca
    41641 gataaccgcc ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg
    41701 tcgggtggaa ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc
    41761 cgacaccgtc gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgctc
    41821 cgccagtcgc cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc
    41881 acagtgcggt acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc
    41941 actcggagaa aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg
    42001 cgtgaccggt acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt
    42061 tcgactgacc gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca
    42121 ggcccatgtg ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg
    42181 cgtcctggtc accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt
    42241 tatcttcatg ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg
    42301 aacgggtcgg gaagacttta gagagcaggg cacaggtctg gccgctctgg gaaatttgca
    42361 gcgccgcgcg catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg
    42421 gcaatttcat cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc
    42481 aacgacaatc accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa
    42541 cacctgccac atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa
    42601 cactttggtg aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact
    42661 ggtggcgaaa aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg
    42721 aacgaggatg aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg
    42781 aggactcctg cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt
    42841 ttacccgctt cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct
    42901 acgacgacgt gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag
    42961 ctgcccataa tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga
    43021 agccactgca ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg
    43081 gacgctatcg ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt
    43141 tttttcacat ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac
    43201 tacgggcaca gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc
    43261 tgcttgcggg tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca
    43321 gatgcgaaaa cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac
    43381 ctggcggcag tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg
    43441 gttttatcct gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc
    43501 taatttgaca atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg
    43561 taaaagttat gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt
    43621 gaccaattca ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag
    43681 tacttgattc gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc
    43741 tgtacccagg ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc
    43801 aggttgatgt gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga
    43861 gaccttaaat ggctgataca aaagcaaaac tcaacctcaa cggggacaca gctgttgaac
    43921 tggatgtgct gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa
    43981 aaggtgtgtt cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta
    44041 cttttattga tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg
    44101 cgaccgattc taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc
    44161 aggaacagta tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga
    44221 ttacccgtct gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta
    44281 ttaccggcgc gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc
    44341 gtgaaattgc cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca
    44401 agtattccat tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact
    44461 tcctgaatat gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac
    44521 gtgctatgga ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca
    44581 ccgtgcgtac cgctggctct tcgggggcga acccgtttgc ctgtatcgca gcaggtattg
    44641 cttcactgtg gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag
    44701 aaattagctc cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt
    44761 ctttccgcct gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg
    44821 taatgcgtga aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg
    44881 aagtggctat ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac
    44941 tgtacccgaa cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt
    45001 ccatgttcac cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg
    45061 aaatgcacag ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa
    45121 aacgcgactt taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt
    45181 aacccgccgt tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa
    45241 taaaatccac tatcggacat tcatatattc taaaaaaaaa tacatcggta taaattataa
    45301 acaaatagat gatttttaat catctatctt cctctcagtt acatgaataa accaccaaat
    45361 atagcgcacc cctataattc ccaatacttt attcatcaaa catttaattc ataccatcaa
    45421 ttaattactt ttacatttaa taaaattaat aatattttta agaaaaacta aaaacattcg
    45481 acaatcaaat aagatattct tgcaccgata tacttttttc ttttctgtta aaggactcag
    45541 ttatgtttaa aggacaaaaa acattggccg cactggccgt atctctgctg ttcacagcac
    45601 ctgtttatgc ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag
    45661 caccgtgtga aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca
    45721 ccagccacat taatcagtca catcacagcg atgccgttgc tgtcgacctg ctcttagtca
    45781 actgtgatct ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa gttgcagtca
    45841 cctttgatag ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg
    45901 gtgaagccac cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat atcgttttag
    45961 gcaccgctac tccagatatc gacctggctc cgacctccag cgaacaaacg ctgaatttct
    46021 ttgcctggat ggaacaaatt gatcaggcta cacctgtaac gccaggcgca gttacggcga
    46081 atgcaaccta cgtgctggat tataaataag tattactttg gcggcgctat cgccgccttt
    46141 cataaaatat ttatcaggta tggacaccgt gaatatttat cgactctctt ttgtatcctg
    46201 cctggtcgtg gcgatgcctt gcgcattggc ggtcgaattc aatctgaatg ttctcgataa
    46261 atcaatgcgc gaccgcattg atatttcatt attaaaggaa aaaggagtca ttgctcccgg
    46321 tgagtatttt gttagcgttg cggtaaataa taaccaaatc agtaacgagc aaaagattaa
    46381 ctggcacaaa aatgacgata aaaccattcc gtgcatcaat gatttactgg tcgataaatt
    46441 tggcttaaaa cctgaagtcc gtcagtcgtt accattgata aatcagtgcg tcgattttag
    46501 ctcccgacct gaaatgctct tcaatttcga tcaagccaat cagcaactga atattagtat
    46561 tccgcaagcc tggctggcgt ggcactcaga aaactggacc ccaccctcca catggaaaga
    46621 aggtgtcgcc ggtgtcctga tggattacaa cttgtttgcc agcagctacc gcccacagga
    46681 tggcagcagc agcactaatc ttaacgccta tggaacagcc ggaatcaaca cgggggcatg
    46741 gcgcttacgc agtgattatc agttgaatca tactgatagc gatgataacc atgaacagtc
    46801 aggcgaaata tcacgtacct atctttttcg cccgttaccg caattaggtt caaaattaac
    46861 cctcggcgaa acggatttta gtcccaatat tttcgacggt ttttcttata ccggcgcggc
    46921 actggcaagt gacgatcgaa tgttgccatg ggaactacgc ggctacgccc cacaaattag
    46981 cggtattgca cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg tcatttacca
    47041 gaaaaaagtc ccaccaggcc catttatcat tgacgacctt aatcagtctg ttcagggcac
    47101 actggatgtc aaagtgacgg aagaagatgg tcgggtgaac aatttccagg tttcagcagc
    47161 atcgacgccc ttcctgactc gtcagggaca ggttcgctat aaactggccg cgggtcagcc
    47221 acggccctcc atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg
    47281 ggggatgctg tcaaacacct cgctgtacag cggcctgctg ctttctggtg atgactacca
    47341 ttctgccgca atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt
    47401 cacgtgggcc agtagccatt ttgatactca gcaggacgag cggggcttaa gctaccgttt
    47461 taattacagc aaacaagtgg atgccactaa cagcacgatt tccctcgccg cttatcgttt
    47521 ctccgatcgt cattttcaca gctacgccaa ctatctggat cacaaataca acgacagcga
    47581 tgcgcaggac gaaaaacaga cgatcagctt atctgtgggc caaccgatta ccccactaaa
    47641 cctcaatctt tacgccaacc tgctacatca aacctggtgg aatgcagacg cctccacgac
    47701 cgccaacatc acggcaggtt ttaatgttga tattggtgac tggagagata tctctatttc
    47761 tacgtcattc aatacaaccc attacgaaga taaagatcgc gacaaccaga tttacctgtc
    47821 gatttcgctc cccttcggta acggtggtcg ggttggctat gacatgcaaa acagtagcca
    47881 cagcaccaca caccgcatgt cgtggaacga tacgctggat gaacgtaata gctggggcat
    47941 gtctgccgga ctgcaatccg accgtcctga caatggagcc caggtgagcg gtaactatca
    48001 gcacctgagt tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag
    48061 ttccgtcagc agcagctgga gcggttcttt caccgcaacc caatatggtg cagcgtttca
    48121 tcgccgcagc tccaccaatg aaccacgcct gatggtcagc accgatggcg tggcagatat
    48181 tccggttcag ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat
    48241 ttccagttac cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac
    48301 agttgcagaa aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcact
    48361 ggcttcccgt tccggtaaag acgttaacgt catcattcgc aacgccagcg gtcagtttcc
    48421 tcccctcgga gcggatatcc gccaggatga cagcggcatt agcgtgggga tggttggcga
    48481 ggaaggacat gcctggttaa gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg
    48541 tgatagccag cattgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct
    48601 gattttacct tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt
    48661 ttgttgttgg ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt
    48721 gtgtttaacg ctaatgataa agctaccagt ctgcgggtgg ataatcgtag cgataaactc
    48781 ccctacctcg cttattcctg gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg
    48841 gtcgccctgc cgccgataca acgtctggaa ccgaaagcaa caacacaggt acggatagta
    48901 aagcaagcct cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg
    48961 cgtgaaattc ctcccgcccc ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt
    49021 cagagtcgta tcaaggtgtt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag
    49081 gtcgaactgc agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc
    49141 gcgtattatc tgaccattgc ctacctggga cgaaatgaaa aaggcgttct ccccggcttc
    49201 aaaaccgtga tggttgcgcc ttttagtacg gtcaacacaa acaccggaaa ttatagcggc
    49261 agccagtttt atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac
    49321 tgcagcggac aatgccattt acaagccgtg gaggcgaaga aatgagtgct ggcaagggag
    49381 tgttgctcgt catctgtctg ttatttctgt cgttgaagtc tgcgcttgcc ctgaactgct
    49441 actttggttc atccggtggc tcggtagaaa aatcagaagc gattcaacct tttgctgtac
    49501 caggcaatgc caaactcggc gataagatct gggaatctga cgatattaaa ataagatctg
    49561 ggaatctgac gatattaaaa ttcccgtcta ttgtgacaac aataccaacg gcaactttga
    49621 aagcgaacat gtctacgtat gggtaaaccc ctacccgggg gtgcaggatc gttattatca
    49681 actcggagta acttacaatg gcgttgatta tgacgccaac cagggaaaaa gccgcatcga
    49741 cactaaccag tgtatcgaca gtaagaacat tgacatttat acccctgatg ggatggcaga
    49801 acaaaatttg ctcgggtgat cccgctaata ttcacatgtc gcgaaccttt ctcgctcgca
    49861 tgcggttata cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt acgcttagcg
    49921 actatatcgt cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct actgcccaaa
    49981 acctgaaata tcatattact ggtctggaaa acattcgcgt gctggattgc agcgtcaatt
    50041 tttccatttc accggaaaca cagatgattg attttggtaa atttaatttg ctggatattc
    50101 gtcgccacac aatgtcgaaa acattcagta ttaagacgac aaaatcacaa aatgaccaat
    50161 gcaccgacgg atttaaagtc agttcctcct tttataccga agaaacgttg gttgaagaag
    50221 ataaagcatt gttgattggt aatggtctga agctacgttt gctggatgaa aacgcctcgc
    50281 cttatacctt caataaatat gctgaatacg ccgatttcac cagcgacatg ttggtctacg
    50341 aaaaaaccta tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag gctgggccct
    50401 tcgatacagt ggtgcttttt aaaattaact ataactgatt tgccatgatg ccctttgcag
    50461 ccggaaaggg catctctttc agaaaattgc tgattttccc cttttccttg attgctgtta
    50521 ttttttccta gcccggcttt ctttatgctg tatcgataca ccaaagagaa aataatgagg
    50581 gagcgaagga tgccagtttt gcagtgggga atgttatgtg tgctatcact tctcctttct
    50641 attggtttcc tcgcggtaca tctcccggcg gcgctattac ttgggccgat gatcgccggg
    50701 atcatcttca gtatgcgcgg aattactctg caacttcccc gctccgcttt tctcgccgcg
    50761 caggccattc ttggctgcat gattgcgcaa aacctcactg gttcgattct caccacgctg
    50821 gccgccaact ggccgatcgt gcttgctatt ttactggtga cgctgctttc cagcgccatc
    50881 gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggtgc ctggggttct
    50941 tctccaggcg gcgcagcggc aatggtcgct atggcgcaag attacggcgc agatattcgc
    51001 ctggtggcgt ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc tgttctggtc
    51061 acccgcatga tgctgggtga taacgctgaa gcggttaacc agcaaatcgt ctggttcccg
    51121 ccagtgagca ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg tacggcggga
    51181 tgcatgctgc gtcttccttc cggcacgatg ctcatcccga tgctggcggg cgcagtgctc
    51241 cagtctggtc agctcatcac catcgaacta ccggaatggc tgctggcgat ggcgtatatg
    51301 gcaattggct ggcggattgg tcttggtttc gataaacaaa tcttactgcg ggcactacgc
    51361 ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc aggtatggcg
    51421 tggggactga cccggtttat gcatattgat tttatgactg cctacctcgc caccagtccc
    51481 ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat ggcgcttatt
    51541 atggcgatgc aaaccctgcg cctgttcagt attttgctga cggggccagc cattgcgcgg
    51601 tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg acaccagtaa
    51661 aacgggcgcg aggacaacgt ggttttctca atgatgccgc cacagcgttc gcacagttcg
    51721 ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatgcttatt ctcatccacc
    51781 tgcccccgcg tagcgtaaga aaaccgagga atctccagta acgcgtgtgc gagtgcatcc
    51841 agttgcgccg cattgagatc tttcgcttta tgatttccag tcaacccaac ctgccagagg
    51901 atctccaccc gcaaataatt gccaagccca gccagaaacg cctgatcgag cagtaatcct
    51961 gcaaactgac ggttacgaaa gcgcggcgac aataaccgtt cctttaccac ctccggcgtc
    52021 agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt
    52081 tccggcctca acatctcaat atccgaggcg ctataaagca gaatggtttt gtcagccgtt
    52141 tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc
    52201 cagacgccgt aaagctgatt atggctgtag agcgttaaac cgttgggaaa atgagttaac
    52261 aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagttg tgattgataa
    52321 gtttttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt gatcgccgcc
    52381 tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat atcctttttt
    52441 cattctttat taagcggggt tatgctgcga caacaatccc ctttttggca aatgtagctc
    52501 gtaatcggcg ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag accgtttgcg
    52561 ccgcgaccgt tgcccattcg ccggtgatac ttttgactct gccgtgttgc accatctcca
    52621 gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc gcctggctga cttcgcggca
    52681 ccagcgagcc gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc gttgtcaggc
    52741 catattgctt gcctgcacga atcagctcgc ttcctgccag cccaacgaga accaatgccg
    52801 gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcttct ttcgccgcct
    52861 ggttgtacaa catgccgtgc ggtttgacat gacgcattac gccgccttgc gcacgggcaa
    52921 tcgttgccag cgcgccaatt tgatacagcg tctgggcgta aacggtttcc ggcggcagct
    52981 gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga
    53041 caccattttt tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa
    53101 atccacaagc aatattggca gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc
    53161 cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacgc taattgttca
    53221 aaataacgtt gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca ctggacaaaa
    53281 tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc aatgatacag
    53341 gcaatacgtg gataaccacc ggtggtctgt gcgtcgttca tcaacacaat cggctgcccg
    53401 ttatgcggca cctgcaccac gcccggtaac aaaccgtgag ataacagttc gcgatcggtg
    53461 atgcgtttta aaatttgccc ctgtaaacga tagcccattc ggttactttg cgagctaagc
    53521 tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg atattccggc
    53581 cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg cgcttccata
    53641 aaatcacgct tcgatttgcc aatcgggagt cagtcaccat ccttcagtaa acggccttcc
    53701 agcccgccaa tccccacttt gagatcggtg ctgcatgacc ccattaccgg cggaacatca
    53761 ataccacccg cgaccgccag ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa
    53821 cgctggcctg ctttcatcgg caatcgccag ccggtccaga cggcgttatc atccaaccgc
    53881 gcttcgcaac ctgcacccgt cagggcaaac cacccatcag tttcgaactc aactgttaac
    53941 tgaccgagcg tgatctccag cgcgggggca ttggcgtcat tacccaccag caggttagca
    54001 atgcgcagcg caggcatatc cagtgcgcca cagtggctga tacccgactg gcaaaaaccg
    54061 tgacgaccgc catcctgcac agtggtgtac atgcccgcac gaataatctt cagcatattc
    54121 cctccttctg cggtacaaat cgcacgctgt ctcccggacg taataagatg ggttcgtcac
    54181 gcgccggatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca cccggcgttg
    54241 ccagcggata aacaccagtc tgcggcccgc cgatcccgac agaacctgcc ggaacgagca
    54301 agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg agatacggga
    54361 agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc aattcaacaa
    54421 cctgtttttc gctcaacccg caatacgccg cgaccaccgc caaatccggt ccgcctgcac
    54481 caccgtaaac caccggaatt tcaataaagc gagactcagg ttccagggcc tcgctctcct
    54541 cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga ttacgcaaaa
    54601 tcaccgtgat attgttcatg ccgggaatgg cttcaaccac attcggcata tccaccagac
    54661 gctgcgccag tcgccagatc cgtttctggc tagccagcgt caccggcggt tccagttcca
    54721 gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc gatccctctg
    54781 attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc ggtattttca
    54841 ttcagccact cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg acctgcagca
    54901 taaaaatgca atccctgctc gcgggctgaa tgaatggtct gttcagaaac ttcgccagta
    54961 ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc gccgcccgtg
    55021 caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa cggcttacgt
    55081 cccagacgcg cttcaatcca ggaggctagt tccattcccg gcacaggcat ggtcagctct
    55141 ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa cgccgccagc
    55201 tgtgcgttat tgcccagctc aggatgtgcg tcaagcggca aatgccagcc atacaggttg
    55261 atatcattcg ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat gaccggagac
    55321 tctcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag acgcactgcc
    55381 tcatcgagca aagcctggct ggcggtgaca ccggtaacaa ttttttgcac cgtctctttg
    55441 ccttccacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg
    55501 ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta
    55561 caccgttaac atagcgttac atcgcctgtt cgtcgatagc caatcttttg acatattaag
    55621 aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca gctaatgtat
    55681 atttatgcaa tcagacagca tattgcataa ataatcagat ctgtatcaaa gttttaactt
    55741 taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat tgtggaatta
    55801 ttatgaataa acacgcatca caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct
    55861 gggaatattt tagcttttac ggcatgcgtg ccctgctgat tctctatctc accaatcaac
    55921 taaaatacaa cgatactcac gcctacgagt tatttagcgc ctactgttcg ctggtgtatg
    55981 tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc atggcggtga
    56041 tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt gagatccatc
    56101 cgtcattcct ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg tttaaatcta
    56161 acgtaagctg tctgctcggc gagctgtatg agccaaccga tccgcgtcgt gatggcggtt
    56221 tctcgctgat gtatgcggcg ggtaacgtgg ggtctattat cgcacctatc gcctgtggtt
    56281 acgcccagga agagtacagc tgggcgatgg gctttggcct ggcggcggtt ggaatgatcg
    56341 cgggtctggt cattttctta tgtggcaatc gtcatttcac tcatacccgc ggcgttaaca
    56401 aaaaagtact gcgtgcgaca aactttctcc tgccgaactg gggatggctg ctggttctgc
    56461 tggtggcaac gcctgcactg attaccgtgc tgttctggaa agagtggtcg gtatacgcct
    56521 taattgtcgc gactattatt ggcctgggtg tactggcaaa aatttatcgc aaagcagaaa
    56581 accagaaaca gcggaaggag ctggggctga ttgtgacgct caccttcttc agtatgttgt
    56641 tctgggcctt cgcacaacag ggcggtagct cgattagcct ttatatcgac cgcttcgtta
    56701 accgcgatat gtttggttat accgttccga ccgcgatgtt ccagtcgatt aatgccttcg
    56761 cagttatgct gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc
    56821 gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc gccggattct
    56881 gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct ctgccactga
    56941 tggtgttagg cctcgcggtg atgggctttg cggaactgtt tatcgacccg gttgccatgt
    57001 cgcaaattac gcgcattgaa attccgggcg tgaccggcgt attaaccggc atctacatgc
    57061 tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag acatcgcagg
    57121 cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt gaagtatttg
    57181 atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt tggctgtatc
    57241 aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc ataataggcc agcaccgatg
    57301 cctgatgcga cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat ttattgaatt
    57361 tgcacaaatt tgtaggctgg attaggcgtt cacgccgcat ccggcatctg ggctcgattg
    57421 cctgacgcgt ctgttatttc cccttccgcg ccgcctcata cgctgccaac gtttgtactc
    57481 tcgcctcttt gtgctcgact atcggttgcg gataatccag cttcacacct gctttctgcg
    57541 cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc ggtaaccatc
    57601 gacgaataaa ctcgccctca cggtcaaatt tctcgccctg agttatcgga ttgaaaatac
    57661 gaaaatacgg cgcagcatca gtacccgtag aagccgccca ctgccagcca ccgttattag
    57721 ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga
    57781 tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta tgcatccagc
    57841 cagtgctgtt aagctgacgc atagcggcat caataatcgg gtatcccgtt ttgcctttct
    57901 gccaggcctg taaatgtgcg ggattgcttt gccactgcac acgatccgtc caggcaataa
    57961 atggacgatg tttacacaac gaggggtaat acgtcatcag atgacggtag aactcgcgcc
    58021 agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc ggctgttccg
    58081 ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccaag ctggcggaca
    58141 aacggctggt gccttccact gccggaaaat ctcgttgttg ctcatactct ccagcaccgt
    58201 tctcgcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag
    58261 tatcgaaaga ctgacgagga taattcagcg taatggatgg cgcgggcttt atcgatccgc
    58321 tactacgaac ttttggtgca gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc
    58381 aggcattctt aaaaggcgta aagactttgt acatctcgtg attgccggtc atcaccgccc
    58441 ccggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc agcgctcttt
    58501 caacgtccac atcccgcgct cgctcattca cctcatactg atagttataa aaaagatgag
    58561 taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca aagtcatcca
    58621 cttcacggaa caataaagga ataccttttt ccgccagcgc gatttgtagc ccattcagtt
    58681 gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtctcccac ttgcgtggtg
    58741 tagcgatata cagcgccagc acgcgtgcag acgaattgcg gcaggcggca gccagtgcga
    58801 gattatcgtg cagacgtaaa tcctggcgaa accaggccag atgggtagtc ataaaactcc
    58861 tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca
    58921 ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa gaagtggttg
    58981 ctcgccacgg cgataactgt cgataagcgc agccagctgc tggcgttctg tggaatcaat
    59041 atgcgggcga aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc
    59101 gggatgagaa agcaaaacaa ttacgcgttg gcggtactgg ttgtaaaaat catccagatt
    59161 ttgccactga tgaatatcag ccacaaacgg gccaagtttg cggtactcgg gctgagaatg
    59221 ggccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc gcgttaagcc
    59281 atgttctttc agtcgatgta actcatgaag ggcgaagata cggattaggg tgctgtcatc
    59341 aaatcgttgt agattcatta ctgctcctga acggtataca ggagtaagtg taggcgttgg
    59401 taaatgatgt ttgtgaagtt aatccgctag cagggcaaga ttacgaaagc gcgctccccg
    59461 caagcaacta atcaaggtag ttaatcatgg ctgaacacga tattacgcca gaagtcacta
    59521 tttccaaaac acagcgccgc tataaagtcg gctatgtcag agcccgccac caggaccgca
    59581 aaaccggtat agcccggtat tattcacaac atcccagtct gcaccttaag ggcgactggc
    59641 tgaaagaggc cggatttgat accggacggg gtgtcacggt gaagatttcg gaggggggcc
    59701 tgaccatcat cgctgacagt gacgaggtgc aggaactgcg ggatgggatg tttagtgtgc
    59761 tgaacgaaag ttaaatatta aaaaagctgg gaagatttaa aaccttccta gctttttatt
    59821 ctgtcttatt ataatggata ctcatcatct tccgatgaat ttataaattg ctccaagttt
    59881 acatgaagag tacttaacct agtttttaac tcattaagtg aagcgataat ttcatatttt
    59941 ccaacataat catatgatgg atcgtattca ttatctatgt cagaagctaa aaatgatgat
    60001 attcgtagtt taaatttagc ttcatcttta ttacattcat tggcataagg tgaatgccct
    60061 atcatagcat attcatctaa tctaaaagga taagatggtt ttaattcatc gatcaatgaa
    60121 atttcaatcc atgttctata tccaggcatt aatggactaa tttctttgag caaaaccttt
    60181 ccacttaata atgtattgag attgttcatt attatgtctc ccacgggaaa aatatatgtc
    60241 taacacttcc accatgttga tgaacttgta aatgtggtaa atcaccatca ggatttcctg
    60301 gcccatgacc tgacactcta tatgaaggtt ttgttacaga aggatctttt atatcatccc
    60361 aatgatatga acctgctttt gttttatttt cagtaagata ttccattaag ttcttagtgc
    60421 tggggcctga ctcacctgtc atatttttat aattatgacc taaatatcta tccataaaca
    60481 attgttcagc gtcacttcta gacctaacag taactttggt ttcactacta tctaataaac
    60541 gattcgcttt ttggttacta acagcacatg gagttaatcc aagtggatca gcatatttta
    60601 gaggattagg tgcataactg tagagattta aaccgcctct caaccctatc gggtcagtta
    60661 ccatgaaccg cccgacatcc ggatcatagt acctgtgcag attgtagtgc agccccgttt
    60721 ccctgtcgag gtactgtccc tggtagcgca ggttctgctc caccccgccg tggtccttct
    60781 cctgtatcgt gttaccccag acctgatacc ccgtctccca cacgattttc ccgtcgctgt
    60841 ccgtcatctc ttccggcgcg ccgttcacat ccgtgtgaaa atacagtatc cggttcgggc
    60901 cctctttgcc cgccttatcc acccgcgcca gcggttcata actcccctgg tccctgtaga
    60961 tgtacaggct gcctgttccc tccggcctgc tctcctgcgc cagccgcatt ccgtcccata
    61021 aaaattttgt ccggttgtac ggcttaccgt cagggctgat gtgctgtttt tccgtccgcc
    61081 tccccagtgc atcgtacaca taccggtatg tcccccgcgc tgtcgtcact tccaccatcc
    61141 ggtgttccgc atcataacgg taactctgcg ttccgctccg gccctccttt tccaccgcac
    61201 ggccaaattc gtcataacgg tagtgcacac cacggtaaga catcagccgg ttgaacggca
    61261 ccacgctgtt ttctgccgtg gccctttccc ccgcgcgcct ccccagcagg tttcccgccg
    61321 cgtccacctc cgtttccggc tggtgccaca ccgggtgcca ctgcgcctgc tccacccgcc
    61381 ccagcgggtc gtgcgtctcc gtgatatgcc cctgcaggtc atacacatag cgccatttgc
    61441 cgccctgagg gtcggtcatc cccagcaaca gtcgttcttc gtcgctccag cggaatgtcg
    61501 tcacctgtcc ggcttcgtcc tggtacgccg tcacctgatt ctgcgcatcc cactggtgat
    61561 aacgtgtgcg ccccagtccg tccgtcacgc acagcctgcg ctgcgccagg tcccacgcca
    61621 gccggtactg ttcaccgctg tccgtgtggt gccccgtcac ccgccatgcc gcgtcatccg
    61681 gaccttcata ccagccccac tggtaataat acccttcgcc gcccggctgg cggtgcagcg
    61741 tcatgcagtg gttttcgtcg taagcaaact cacgcagcag gtgccccgtg ctgtcgtaca
    61801 cgccgttcag gtggccggcc tcgtcatagc tgtaacgacg cagcagtaaa ggtgcagtct
    61861 cagggaaatg ctgataaatc tgtgtcaccc gccggggatg cgccgccagt tcgtaataca
    61921 gacggatgca cggacgctgc tgttcgccgc tgatttccac aatgcggccc cggtcgtcgt
    61981 agtacagatt cagacagttg ctgttacggt cgcccagcat tttcagccgc tggcgctgcg
    62041 ggtggtgcgg gtcagcctca aacagaaaac actgcccgtc atcgctgctg ataagccagt
    62101 gcccctgctc accacgcctt accgccagcc cttcaccggg gctgtaaaac ccgccccccg
    62161 gcagtacggc ttccatatcc aagcgtcgcc ccatgcccga cacaaaggtc atgcagtttt
    62221 cgtctgcgct acccggtgtg cacacgaggc tgacttcata cattacactc cagccctgac
    62281 caaacattcc ttccgtacgt tcgtcacgac tgctgtacac gcgctgccag tccagcggaa
    62341 agtggcccgg cagactgaaa tcgaagtcat cgtcatcggc caggtatttg gccccggtgg
    62401 cggcattgac cggatgcgga agggcactgg tggccttctg cgtcagcatg ctcatcccga
    62461 cactggcagc aaaacaggcg attttagaaa gcattttgcc cgggcgaaga aacatcagcg
    62521 ccacggttgc caccagggtt atctgcgacc tgccgcttct gatatccctg accaccagta
    62581 acggaccgcc aatgcgcaca tccggtgaca caaacgcccc gttccccggc tcattcacca
    62641 ccctggcctc gcaggtacag cgtgccccgc tgcgtgtcgc cggctggctg ttgatacgca
    62701 catcccgcgc gccttccgcg atatattccg ctgcgggcgg ctcgtcatcg cctttgaaca
    62761 gattactgat gctgccaata accgcattca ctccctgaaa ggcggtttcc agcataccgc
    62821 ccggattcag caggttaagg gccgttccca gggggtcatc cagaaaggcg gctttttttt
    62881 gtgcgagaaa ctgcgtcagt gaggaaggat gtttatggca gtcgatttta tcgtcagggg
    62941 tcggggtgga tcccggggcc gcactggcca cagtgggctg ccatagttcc gacacaaaat
    63001 gtgtcacgcc atccaccatt tcagaacgcc cttcttccgt actgagctgg tcgtgcagag
    63061 cccgggacat cagcacaggc cctgtcaccg ggttaaccgc cagggctatg gcccccgcca
    63121 tttccagata ttccccggcc acatcccgga acgttctggc atcctcctga cgctgcgctt
    63181 cttcctgttc tgccttcgcc tgcgcttcct gcgcctctat gtcctgccgc gatgccgcag
    63241 tcgccgctgc ccgcgcggca ggtttgctgt tgatccatgt gtcaccagag ccggtgagga
    63301 tgtaaccttc aatctgaggc gggaaaagca tatttgcgag ggctgaacag gcttcgctga
    63361 ccttttctgt cacgccggtg tacaccatcg ccacacccgc cacaaaaccg gtcagcacga
    63421 cggccgtgca ccccatcgtg atccccactg acgtcgccag taacgaggtt gtgccgacaa
    63481 tgagggcatc aataacgaca tttgcggcca cctccagcac cgcccccagc acatctgcca
    63541 taaacgacgt atgcagcagg gcatcaccct gtcttgccgc ccagaatgcc tcttccatgt
    63601 cccgttccct gtatgattaa ttcgtcctga cttcctgccg catcctgaag cgcagattct
    63661 ttttaatttc agcccagcgc gtcatatctg ccggggtcag cggtgtggtt ctggcgtagg
    63721 tgaaggccat catgacccgc tcacccggca atacaaatgc cacctgacac tggtacacct
    63781 gctcgttacc ccggagaaac tggctctgta tttccaaacc gcctcgttga gataatattc
    63841 cgtcataatg taaatagtcc gttttaataa aggaattatt tattgacagg ttctgttttt
    63901 ttaatcatga atgtaatacc catcaccgct ttacagcaca ctttatcttt ccagtcataa
    63961 tcactaataa tattaagcaa ttagcaatat ttacaacgac cagagcggat acatagttat
    64021 gtatttaaca
//
LOCUS       DAJTRN010000011        60166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRN010000011 DAJTRN010000000
VERSION     DAJTRN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            182..976
                     /gene="panB"
                     /locus_tag="OO328_000722"
     CDS             182..976
                     /gene="panB"
                     /locus_tag="OO328_000722"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5929395.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            988..1839
                     /gene="panC"
                     /locus_tag="OO328_000723"
     CDS             988..1839
                     /gene="panC"
                     /locus_tag="OO328_000723"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5929396.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(1913..2815)
                     /gene="rpnC"
                     /locus_tag="OO328_000724"
     CDS             complement(1913..2815)
                     /gene="rpnC"
                     /locus_tag="OO328_000724"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5929397.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3089..3469
                     /gene="panD"
                     /locus_tag="OO328_000725"
     CDS             3089..3469
                     /gene="panD"
                     /locus_tag="OO328_000725"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5929398.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3473..4702)
                     /locus_tag="OO328_000726"
     CDS             complement(3473..4702)
                     /locus_tag="OO328_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5929399.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4766..5206)
                     /locus_tag="OO328_000727"
     CDS             complement(4766..5206)
                     /locus_tag="OO328_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5929400.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5311..6081)
                     /locus_tag="OO328_000728"
     CDS             complement(5311..6081)
                     /locus_tag="OO328_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5929401.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6078..7004)
                     /locus_tag="OO328_000729"
     CDS             complement(6078..7004)
                     /locus_tag="OO328_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5929402.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7113..7775
                     /gene="can"
                     /locus_tag="OO328_000730"
     CDS             7113..7775
                     /gene="can"
                     /locus_tag="OO328_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5929403.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7816..8352)
                     /gene="hpt"
                     /locus_tag="OO328_000731"
     CDS             complement(7816..8352)
                     /gene="hpt"
                     /locus_tag="OO328_000731"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5929404.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8558..10948
                     /gene="gcd"
                     /locus_tag="OO328_000732"
     CDS             8558..10948
                     /gene="gcd"
                     /locus_tag="OO328_000732"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5929405.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(10995..12545)
                     /gene="cueO"
                     /locus_tag="OO328_000733"
     CDS             complement(10995..12545)
                     /gene="cueO"
                     /locus_tag="OO328_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5929406.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12711..13058
                     /locus_tag="OO328_000734"
     CDS             12711..13058
                     /locus_tag="OO328_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5929407.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13164..14030
                     /gene="speE"
                     /locus_tag="OO328_000735"
     CDS             13164..14030
                     /gene="speE"
                     /locus_tag="OO328_000735"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5929408.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14046..14840
                     /gene="speD"
                     /locus_tag="OO328_000736"
     CDS             14046..14840
                     /gene="speD"
                     /locus_tag="OO328_000736"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5929409.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14878..15240)
                     /locus_tag="OO328_000737"
     CDS             complement(14878..15240)
                     /locus_tag="OO328_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5929410.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15415..18012)
                     /gene="acnB"
                     /locus_tag="OO328_000738"
     CDS             complement(15415..18012)
                     /gene="acnB"
                     /locus_tag="OO328_000738"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5929411.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVSVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18367..20220
                     /locus_tag="OO328_000739"
                     /pseudo
     CDS             18367..20220
                     /locus_tag="OO328_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20291..21715)
                     /gene="lpdA"
                     /locus_tag="OO328_000740"
     CDS             complement(20291..21715)
                     /gene="lpdA"
                     /locus_tag="OO328_000740"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5929412.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22040..23920)
                     /gene="aceF"
                     /locus_tag="OO328_000741"
     CDS             complement(22040..23920)
                     /gene="aceF"
                     /locus_tag="OO328_000741"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5929413.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(23935..26598)
                     /gene="aceE"
                     /locus_tag="OO328_000742"
     CDS             complement(23935..26598)
                     /gene="aceE"
                     /locus_tag="OO328_000742"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5929414.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26759..27523)
                     /gene="pdhR"
                     /locus_tag="OO328_000743"
     CDS             complement(26759..27523)
                     /gene="pdhR"
                     /locus_tag="OO328_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5929415.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28064..29437
                     /gene="aroP"
                     /locus_tag="OO328_000744"
     CDS             28064..29437
                     /gene="aroP"
                     /locus_tag="OO328_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5929416.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29480..30334)
                     /gene="ampE"
                     /locus_tag="OO328_000745"
     CDS             complement(29480..30334)
                     /gene="ampE"
                     /locus_tag="OO328_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5929417.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30331..30882)
                     /gene="ampD"
                     /locus_tag="OO328_000746"
     CDS             complement(30331..30882)
                     /gene="ampD"
                     /locus_tag="OO328_000746"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5929418.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            30970..31863
                     /gene="nadC"
                     /locus_tag="OO328_000747"
     CDS             30970..31863
                     /gene="nadC"
                     /locus_tag="OO328_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5929419.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32066..32506
                     /gene="ppdD"
                     /locus_tag="OO328_000748"
     CDS             32066..32506
                     /gene="ppdD"
                     /locus_tag="OO328_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5929420.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32516..33663
                     /gene="tadA"
                     /locus_tag="OO328_000749"
                     /pseudo
     CDS             32516..33663
                     /gene="tadA"
                     /locus_tag="OO328_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33653..34855
                     /gene="hofC"
                     /locus_tag="OO328_000750"
     CDS             33653..34855
                     /gene="hofC"
                     /locus_tag="OO328_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5929421.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(34890..35933)
                     /gene="guaC"
                     /locus_tag="OO328_000751"
     CDS             complement(34890..35933)
                     /gene="guaC"
                     /locus_tag="OO328_000751"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5929422.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36095..36133)
                     /locus_tag="OO328_000752"
     CDS             complement(36095..36133)
                     /locus_tag="OO328_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929423.1"
                     /translation="MIIKTLKMSARK"
     gene            36158..36778
                     /gene="coaE"
                     /locus_tag="OO328_000753"
     CDS             36158..36778
                     /gene="coaE"
                     /locus_tag="OO328_000753"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5929424.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36778..37521
                     /gene="zapD"
                     /locus_tag="OO328_000754"
     CDS             36778..37521
                     /gene="zapD"
                     /locus_tag="OO328_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5929425.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37531..37728
                     /gene="yacG"
                     /locus_tag="OO328_000755"
     CDS             37531..37728
                     /gene="yacG"
                     /locus_tag="OO328_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5929426.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(37828..38217)
                     /gene="mutT"
                     /locus_tag="OO328_000756"
     CDS             complement(37828..38217)
                     /gene="mutT"
                     /locus_tag="OO328_000756"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5929427.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38277..40982)
                     /gene="secA"
                     /locus_tag="OO328_000757"
     CDS             complement(38277..40982)
                     /gene="secA"
                     /locus_tag="OO328_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5929428.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41044..41556)
                     /gene="secM"
                     /locus_tag="OO328_000758"
     CDS             complement(41044..41556)
                     /gene="secM"
                     /locus_tag="OO328_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5929429.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(41787..42704)
                     /gene="lpxC"
                     /locus_tag="OO328_000759"
     CDS             complement(41787..42704)
                     /gene="lpxC"
                     /locus_tag="OO328_000759"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5929430.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(42805..43956)
                     /gene="ftsZ"
                     /locus_tag="OO328_000760"
     CDS             complement(42805..43956)
                     /gene="ftsZ"
                     /locus_tag="OO328_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5929431.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44017..45279)
                     /gene="ftsA"
                     /locus_tag="OO328_000761"
     CDS             complement(44017..45279)
                     /gene="ftsA"
                     /locus_tag="OO328_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5929432.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45276..46106)
                     /gene="ftsQ"
                     /locus_tag="OO328_000762"
     CDS             complement(45276..46106)
                     /gene="ftsQ"
                     /locus_tag="OO328_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5929433.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46108..47028)
                     /gene="ddlB"
                     /locus_tag="OO328_000763"
     CDS             complement(46108..47028)
                     /gene="ddlB"
                     /locus_tag="OO328_000763"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5929434.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47021..48496)
                     /gene="murC"
                     /locus_tag="OO328_000764"
     CDS             complement(47021..48496)
                     /gene="murC"
                     /locus_tag="OO328_000764"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5929435.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48550..49617)
                     /gene="murG"
                     /locus_tag="OO328_000765"
     CDS             complement(48550..49617)
                     /gene="murG"
                     /locus_tag="OO328_000765"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5929436.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49614..50858)
                     /gene="ftsW"
                     /locus_tag="OO328_000766"
     CDS             complement(49614..50858)
                     /gene="ftsW"
                     /locus_tag="OO328_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5929437.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(50858..52174)
                     /gene="murD"
                     /locus_tag="OO328_000767"
     CDS             complement(50858..52174)
                     /gene="murD"
                     /locus_tag="OO328_000767"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5929438.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52177..53259)
                     /gene="mraY"
                     /locus_tag="OO328_000768"
     CDS             complement(52177..53259)
                     /gene="mraY"
                     /locus_tag="OO328_000768"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5929439.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53253..54611)
                     /gene="murF"
                     /locus_tag="OO328_000769"
     CDS             complement(53253..54611)
                     /gene="murF"
                     /locus_tag="OO328_000769"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5929440.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54608..56095)
                     /gene="murE"
                     /locus_tag="OO328_000770"
     CDS             complement(54608..56095)
                     /gene="murE"
                     /locus_tag="OO328_000770"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5929441.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56082..57848)
                     /gene="ftsI"
                     /locus_tag="OO328_000771"
     CDS             complement(56082..57848)
                     /gene="ftsI"
                     /locus_tag="OO328_000771"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5929442.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSVLVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(57864..58229)
                     /gene="ftsL"
                     /locus_tag="OO328_000772"
     CDS             complement(57864..58229)
                     /gene="ftsL"
                     /locus_tag="OO328_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5929443.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58226..59167)
                     /gene="rsmH"
                     /locus_tag="OO328_000773"
     CDS             complement(58226..59167)
                     /gene="rsmH"
                     /locus_tag="OO328_000773"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5929444.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59169..59627)
                     /gene="mraZ"
                     /locus_tag="OO328_000774"
     CDS             complement(59169..59627)
                     /gene="mraZ"
                     /locus_tag="OO328_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5929445.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14383 a        16680 c        14796 g        14307 t
ORIGIN      
        1 gatggaacta taccaataga agctggtgag tttaaagcca ccagtacttt ccaggtaacc
       61 tacccttaat aagtccaccg caccgccatc atctggcggt gcggtaattg ataaatcatt
      121 gcccccacca atgtgactga taaaatgcca ggcaacacga catcatttat caggatacgt
      181 tatgaaaccg accaccattg cttcacttca gaaatataag caggataaaa agcgtttcgc
      241 gaccatcacc gcttacgact acagcttcgc gaaactattt gctgatgaag ggcttaacgt
      301 catgctggta ggcgactcgc tgggcatgac cgttcagggc cacgattcca ctctgccagt
      361 aaccgttgct gatatcgcct accacactgc cgccgtacgt cgcggcgcac caaactgcct
      421 gctattggct gacctgccgt ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc
      481 cgcaacggtt atgcgtgccg gtgccaatat ggtcaaaatt gagggcggtg agtggctggt
      541 cgaaaccgta aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact taggtttaac
      601 cccacagtca gtgaatattt tcggtggcta caaagtacag gggcgcggca atgaagcgag
      661 cgatcgacta ctcagcgatg cattagcctt agaagccgcg ggggcacagc tgctggtgct
      721 ggaatgcgtg ccggttgagc tggcaaaacg tattaccgaa gcactggcga tcccggttat
      781 tggtattggt gcaggcaacg tcactgacgg gcaaatcctc gtgatgcacg acgcctttgg
      841 tattaccggc ggtcacattc ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat
      901 ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttatc cgggcgaaga
      961 acacagtttc cattaaggag tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt
     1021 cagcaaattc gccgcctgcg tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt
     1081 aatctgcaca atggccatat gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc
     1141 gtcgtcagta ttttcgttaa cccgatgcag ttcgaccgcc cggaagatct ggcacgttat
     1201 ccacggacct tgcaggagga ctgcgagaag ctaaacaaac gtaaagtgga tttagttttc
     1261 gccccttcgg taaaagagat ctacccgaac ggtactgaaa cccacactta cgttgacgtt
     1321 cctggccttt cgaccatgct ggaaggtgcc agccgtccgg gacattttcg cggcgtttcg
     1381 actattgtca gcaagctgtt caacctggtc cagccggaca tcgcctgctt cggtgaaaaa
     1441 gattttcagc aactggcgtt gatccgcaaa atggttgccg atatgggctt cgatattgag
     1501 attgtcggtg tgccaattat gcgcgccaaa gacggtctgg cgctaagttc ccgtaacggt
     1561 tatctgacgg cggaacaacg caaaattgcg cctggtctgt acaaagtttt aagttcgatt
     1621 gctgacaaat tgcaggctgg ggaacgggat ctcgatgaaa ttattaccat tgcggggcaa
     1681 gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc cgacacattg
     1741 ctggaagttt ctgaaaccag caaacgggca gtaattctgg tagccgcctg gcttggcgat
     1801 gctcgcctga tcgacaacaa aatggtcgag ctggcgtaat acttaactgg cgctacggct
     1861 gatggcgcca gttattaatt taccccacgt catctgtcat aacacaggta acttaaataa
     1921 ggttaattac cttatcaatc tcagcaagag gtaaatttgc catctctgca acgtcttccc
     1981 gagacattcc tttacttaga agacgcaggg cgaattcttg cctttcctcc tgtcttccct
     2041 gctgaatccc cttctcaatc cctttctctt caaaccactg cgccagcgtc atcatagact
     2101 tccctcccgt ttccctgtct ctcagcacac cataaaacaa atccgcttgt tcagtatgac
     2161 cgcgttgcag catatagttt tgcatggcaa ctaactgact tccgctagtg tacccttcgt
     2221 caatcagcgt gaccagttgc tccagcaata acattaagtc gcgctggcga atatgttttt
     2281 gcagtagttc gagaatcgca atccgccgat gttgcatgat ttcgtcatcc ggtgtgatgg
     2341 tgatatccac cagcgggaaa ggactgttat agacgcgtcg cgccagctcc ggcgagtaaa
     2401 acatatcaaa ccagcacatt gagagcggat aaggtgtggc ctcgccctga taaaacagta
     2461 tcggcaccac cagcggcagc ttatcgtggc cagcctccag atgccggtgc atggcagcaa
     2521 tagaataacg catcatgcga aaggccattt tcttatccgg cttgctttgg tgttcaatca
     2581 caacatgcag ataaccggga ttgccctgca tttgcatgga atagagcacg tccgtgctgt
     2641 gacctttcag gcactcttca atgaaactcc ccgactctaa atgaagcgtg ttgagatcac
     2701 aaagttcgcg taattccact ggcaaatgta tctccagaaa gtcgcgagcc gtctccgcat
     2761 gcattaaaaa ttgtttaaat accgcgtcat gcggtgtggt actcggtgca tccatcttcc
     2821 ctgtcttccg tgtggccttg cgtgaaggca gtatccagtc aaggcgtgct atcgtcagcc
     2881 acctctttat tgtttaccga gcagcgttca taaaaatatc aattcgttac aggcgttaca
     2941 tggcacgctt cggcgcggta cgcaaactgc tgacgcaaat gccatcagat gagtagacac
     3001 tcatcaaaaa tcgggcaata ctgcgtgaga atttcctcaa agcaggcacc gcctgcttcg
     3061 ttaacgacag ggtagaaagg tagaagttat gattcgcacg atgctgcagg gcaaactcca
     3121 ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt tcttgcgcca ttgaccagga
     3181 ttttcttgac gcagccggta ttctcgaaaa cgaagccatc gatatctgga atgtcaccaa
     3241 cggcaagcgt ttctccactt atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt
     3301 taacggtgcg gcggcccact gcgccagcgt cggcgatatt gtcatcatcg ccagcttcgt
     3361 taccatgcca gatgaagaag ctcgcacctg gcgacccaat gtcgcctatt ttgaaggcga
     3421 caatgaaatg aaacgtaccg cgaaagcgat tccggtacag gttgcttgat tgttatccct
     3481 gcggctggtt actcaccagc cgcgacatct tctccagcga atccgttctt aagatataaa
     3541 gtcgttttag taacaacgga ttatcccccg gttttacttt gcctttcatg gttgtcaccg
     3601 ccaggtgaaa tccggcatcg tttgctgcct tcacggcttt gtcattaaag ccgccaaacg
     3661 gatacgaaag ataccagaca tgtggattaa attgcgccag agcgcggcgt gaacgtgcaa
     3721 aatcaaacag aatattgtgc tcactacggc tcagtaatat gggtcggcga taaccatcta
     3781 cccgatgcaa aaaatgggta tgtgactgga aatcaaaaac atcgcgaatt tcgttaagct
     3841 cagaaacgct cataaattgc agcgattttg ggttccactt ctgcgggtga cgtttgatgc
     3901 gtgaggtaac aataaacacc gtcgccttca tgccatattg tttcaacaca ggatacgcat
     3961 agcggctcac cgacttgagg ccatcatcaa aggtaatcac caccgctcgc gcagggagat
     4021 tgatcgtatt cttcacgtaa ccttccagct gagccatgct cagtgtcgcg tatcccctgt
     4081 cgcgcagcca ggccatttgg ttattgaaag cgcgtaccga ggtggtcgtc gaagtatggc
     4141 gaaaacgggt gttttcttcg tcgcgcagaa tatggtgata agttagcacc ggcaggccat
     4201 tatcgggttg ggcatccagc gcgctgatat aggccagtcg atcgccaata cggatctgat
     4261 accaggtttg atttaacctg tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg
     4321 ccagtacccc aaatggcgca ctgcccacac tcggcgcgtt gtagaccggc gtatctttcc
     4381 aggtaattaa gttctgatta ctcagcggct tgttgaggtc acccaaaccg tcttcaactt
     4441 tttgtcgtcc ctgaactggc tcgagatgcc ctttatcgat aaacccttta ccaaagccaa
     4501 aattaaatgc gtaataactt gcggcagtgg gctccacggc aatgatttgt ccggcccgaa
     4561 tattccccac ggtcaccatc ttgtcactaa tttgcgccca gaccgcagca ttttcgatgg
     4621 tttgcatata acgggcaggt aacgcggcac tgacacttgc ggtaaacagc atcagcagga
     4681 gaataacagc ttgtttatac atgaaaaaat aaccgagggt gaaacaacgc ttttattgta
     4741 gcaaaagcgc catcagaatc gcgggttaat actcatacaa attatgtttg aggacgaaag
     4801 gcggattttt ttgttgttgg tgccaaagac tactcacctc cggcccaccc agttcgacaa
     4861 tacactcacg aaactctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta
     4921 acggtaacgt gacaccagaa atcacttcgc aacgggagtg tttgtggctt aataatgaag
     4981 ccacgcgata cggtggcgct ccggctatat cggttaagaa gatgacaccc tcaccagagt
     5041 ccgcttcatg cagagcatcg cacatcatgc ggctgagcat attagagctt aatccgcgcc
     5101 agaaattcac ggcccggcac tgaagaagtg ccccatgttt tttctccagc gcatccagta
     5161 tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta
     5221 gccgaaaatc agcctaccct gaccattgtt gataaagggt gatagccgtc aaaactcctc
     5281 tcccccgaat ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat
     5341 cagcgaccaa cagatcaaat aaaacgccac aataaagacc accagtacgc caaaggtagt
     5401 gaccagcggc acatcattta taccgaggaa gccgtagcgg aaaccactga tcatataaac
     5461 gattgggttc aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gtgagtaaaa
     5521 gaccccgccc aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc
     5581 gaacgttttg gcaaacacgc cgttcagcaa acccgcaagg gagaatagca ccgccgtgag
     5641 caccagggtt aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga
     5701 aattgccgtc acaagaatgc caacaaacag accacgcgcc acgccaccgc cgacatatcc
     5761 ggcaataatg acgtgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa
     5821 cttggcacca aaaaatgatg acgcgacgtt agcgtaggcg ttggtgatca ccgacatcat
     5881 gatcagcccc ggtacgatga actgcatata gctgaagcca tgcatatcgc caatacgcga
     5941 acctatcagg ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag
     6001 cgtctgcacc cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc
     6061 cacccagtaa agatgcatca tgcgcgatct ccttgttttt cattaaccag tgaaacaaac
     6121 agctcttcca gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt
     6181 aactgcgtaa atacgctgtt gatcccctgc tcacgtagca cttcaacttc cagcgtcgcg
     6241 gtatcgacca gtcgatactg atagccatcg agcttcggta acggactttt cggtgcgaga
     6301 tcgagaataa aggtttccga tttcagcttc gccagcagcg ccttcatcga ggtattttcc
     6361 accagctcac catgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg
     6421 tagtgtgtgg taagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac
     6481 attgagcggc gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta
     6541 ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata
     6601 cgcgcacgtt cgttgcgttt tccccataaa tcgagctgct taagatattt ttcgctgcgg
     6661 atgtacgcct ctttgcgctc cacaccgtag taccctgcct gattcaccac aatttgctgc
     6721 acggtttcaa acgggttgaa gttaaattcc tgcggcacca gtcccaactg acgtttagcg
     6781 ttcacgacat ctttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt
     6841 accagagagc tgataatacc gatagtggtc gatttcccgg ccccgttcgg cccgagaagc
     6901 gcataaaaat cacccgcttc gacctgcaaa tctattccac gaagcgcctg aacgccgcct
     6961 ggatagtttt ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct
     7021 tacgttctta cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa
     7081 cgtaattatt tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca
     7141 acaatgcact atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg
     7201 cacaagcgca aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc gttcctgcag
     7261 aacgtttaac cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctag
     7321 tcattcacac cgacctgaac tgcctttccg tggttcagta tgcagtggat gtactcgaag
     7381 ttgaacacat tattatctgt ggccactacg gttgcggcgg cgtacaagcc gccgttgaaa
     7441 acccggaact ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac
     7501 atagctcatt gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga
     7561 acgtcatgga acaggtgtat aacctgggcc actccaccat tatgcaatca gcgtggaaac
     7621 gcgggcagaa agttaccatt cacggctggg cctacggcat tcacgacggc ttgctgcgtg
     7681 atctggatgt taccgccacc aaccgcgaaa cccttgagca acgttaccgt cacgggattt
     7741 ccaacctcaa gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat
     7801 ccggcaactt cacacttact cgtccagcag aatcactttg ccaatatacg gcagatgacg
     7861 gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc
     7921 gataaattca accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat
     7981 cgccaacgac ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc
     8041 cgagtcgatg atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc
     8101 tttgaggatt ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat
     8161 aaagtcgact tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga
     8221 gccacgcagc agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat
     8281 ctgacgaccc agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac
     8341 agtatgtttc atatctctaa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt
     8401 ttggcaccgc aacaaagcgc aagacgcgca gtataccagt aaaactattc attcgcggaa
     8461 tctgttaata aagctcgaat tgtgatgacg gtcacacatg ttaaaaccta taattaattg
     8521 ctactaaaat attaatgaat tgaaatggtg tctctttatg gcaattaaca atacagcctc
     8581 gcgacgatta ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc tgtatctact
     8641 cattggcgga ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggcct
     8701 tgtgatgctc ggcgtcgcct ggatgctgtg gcgcagtaaa cgcgccgcgc tttggctata
     8761 cgcagccctg ctgctcggca ccatgatttg gggcgtctgg gaagttggtt tcgacttctg
     8821 ggcgctgact ccgcgcagcg atattctggt cttcttcggc atctggctga tcctgccgtt
     8881 tgtctggcgt cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtcgcact
     8941 gctgattagc ggtggtatcc tgacctgggc cggatttaac gatccgcagg agatcaacgg
     9001 caccttaagc gccgatgcca cacctgccga agctatctcc cccgttgccg atcaggactg
     9061 gcctgcgtat ggccgcaatc aggaaggtca acgcttttca ccactgaagc aaattcacgc
     9121 cgataacgtc cacaagctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc
     9181 gaacgatccg ggtgaaatca ccaatgaagt gacgccgatt aaagtgggcg atacccttta
     9241 cctgtgtacc gctcaccagc gcctgtttgc gcttgatgcc gccagcggca aagagaaatg
     9301 gcattacgat cctgagctga aaaccaacga gtctttccag cacgtaacct gccgtggcgt
     9361 ctcttatcat gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg
     9421 tcgtatcatt cttccagtga atgatggtcg cctgattgcg attaacgctg aaaacggcaa
     9481 actgtgcgaa accttcgcca ataaaggcgt gctcaatctg caaagcaata tgccagacac
     9541 caaaccgggt ctgtatgagc cgacttctcc gccaattatc accgataaaa ccatcgtgat
     9601 ggccggttca gtcaccgata acttctcaac ccgcgaaacg tctggcgtga tccgtggttt
     9661 tgatgtcaac accggggagc tgctgtgggc ttttgatccg ggcgcgaaag atccgaacgc
     9721 aatcccgtca gacgaacaca cctttacctt taactcacca aactcgtggg caccagcggc
     9781 ctatgacgcg aagctggatc tggtctatct gccgatgggc gtgaccacgc cggatatctg
     9841 gggcggtaac cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc
     9901 cactaccggg aaactggcgt ggagctacca gaccgttcac cacgacctgt gggacatgga
     9961 tcttccggca cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccgattat
    10021 ttacgctccg gcgaaaaccg gtaacatttt tgtgctcgat cgtcgtaatg gcgaactggt
    10081 ggttccggcc ccggaaaaac cggttcccca aggtgctgcc aaaggcgatt acgttacccc
    10141 tactcaacca ttctcggaac tgagcttccg tccgacgaaa gatttgagcg gtgcggatat
    10201 gtggggagcc accatgtttg accaactggt gtgccgcgtg atgttccacc agatgcgcta
    10261 tgaaggcatt ttcacgccgc catctgaaca gggtacgctg gtcttcccgg gtaacctggg
    10321 gatgttcgaa tggggcggga tttccgttga tccaaatcgt gaagtggcaa ttgccaaccc
    10381 aatggcactg ccgtttgttt cgaaactgct cccgcgtggt cctggcaacc cgatggagca
    10441 gccgaaagat gccaaaggca cgggtacgga atccggcatt cagccacagt acggtgtacc
    10501 gtatggtgtc acgctcaacc cgttcctctc accatttggt ctgccatgta aacagccagc
    10561 atggggttat atctcggcgc tggatctgaa aactaatgaa gtggtgtgga agaaacgtat
    10621 tggtacgccg caggacagta tgccgttccc gatgccggtt ccggtgccgt tcaatatggg
    10681 tatgccgatg ctgggcgggc caatctccac ggcgggtaac gtgctgttta tcgccgctac
    10741 ggcagataac tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcagggtcg
    10801 tttaccggcg ggtggtcagg caacaccgat gacctatgaa gtgaatggca agcagtacgt
    10861 ggtgatctcc gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc
    10921 ttatgccctg ccggatgatg tgaaataacc ataatgcccg gagagatccg ggcatatttc
    10981 cgaatacggt ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc
    11041 agatggcagt gcgccatata agcatgttct ttcggtgcgt tgtgattaaa cttcaccagc
    11101 acttcactga cattgccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct
    11161 ggcggtttgc cattttctga caagatacgg aactgcgtgc cgtgaatatg gaacggatgc
    11221 agcatcatgt cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca
    11281 aacatcggct tattcatatc aaacgcctga ccgttgattt tattggcatg gtggaaatcg
    11341 aacttcccgc cgtggttcat atgattcata ttgccgtgcc ccatatggcc catcatctgg
    11401 ctgtgatcca ttccggccat cgcctgatcg ccatatttct ccattagcat ctgcatcccc
    11461 atcatatcga gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag cccttccagc
    11521 gaaggtaacg caggcaggct acttaatgtg tcaggcaaag caccggaggc actaatagca
    11581 atcggctgaa tccgcattac cggatgcggc ttgtcaaacg gtgcaatcgc catccccatc
    11641 tggctgaccg gcagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact
    11701 tcaaaacgct cgcccatcag caccggcagc tcgctcacct tcaccggttc aggtagcaga
    11761 ccaccgtcgc tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag
    11821 cgggcattac agccattgag caaacgcagg cgcagccaac cacgcggggc agcgtgttgt
    11881 gggtagattg caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc
    11941 acatccagtt gataatcaat ctgcccgtcg gcgctaaatt tcttatcctg aacgatcacc
    12001 ggaacatcat cgatacccca ctgttttggc agcattaatt tcaggatctc gtcatcttca
    12061 atcaccacca gaccagccag ccccatcgcc acctgtcgcc cggtcttgcc atgttgatgt
    12121 ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca
    12181 cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc cagcccgtgc
    12241 cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac cgctttgccg
    12301 cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt
    12361 ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt acgggcatcg
    12421 gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcacggctc
    12481 cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa atcacgacgt
    12541 tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc
    12601 ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc
    12661 tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt
    12721 ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt tacgcgctca
    12781 gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg
    12841 attgtggtta ccagaactta tctaacgggc aaattcgtcg cgcactggtc tttttcgccc
    12901 agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct ctcggtgaag
    12961 acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc aaagccctgg
    13021 cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa
    13081 atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt
    13141 gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg
    13201 accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc
    13261 aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag
    13321 tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac
    13381 tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc
    13441 gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg
    13501 tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc
    13561 gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg
    13621 atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg ttcacctcag
    13681 cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg
    13741 gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact
    13801 tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt
    13861 ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc
    13921 gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt
    13981 ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata
    14041 agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt
    14101 gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata
    14161 tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta
    14221 tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca
    14281 ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg
    14341 gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc gtacatacct
    14401 acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta
    14461 cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg
    14521 atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac ggtatgaagc
    14581 actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc
    14641 tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc aagatgttgc
    14701 ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca
    14761 gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc
    14821 gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta
    14881 cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca
    14941 tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa
    15001 ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg tgtattcatg
    15061 ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc
    15121 atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg
    15181 ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat
    15241 agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg atgtaattat
    15301 cgcagcctta ccggagaaaa acagcggaac ggcgatagtt tagaaataaa aaatgcccgg
    15361 gaatctcatt caccgggcat tgtgtcgttt atgcgcagcg cgtgcgctta cttattacac
    15421 cgcagtctgg aaaatcaccc catcggcttt ttcggtgtac tgagaaagct ggttgaagtt
    15481 cagataacga taagtatcaa cggcagtttt atctacttgc gcaacgtagg tctggtactc
    15541 ttccggcgtc ggcagtttgc caatcagcgc cgcaacagcc gccagttctg cagaagccag
    15601 gaagacattc gcgccagtac ccaaacggtt cgggaagtta cgggtagagg tggaaaccac
    15661 cgtcgcaccg tctgctacac gcgcctggtt acccatacac agggaacagc ccgggatctc
    15721 gatacgagcc ccgctcttac cgaagacgct gtagtagcct tcttccgtca gttgcgcggc
    15781 gtccatacgg gttggcggtg ccacccacag gcgggtcggc aactgacctt tgtgcgcatc
    15841 cagcagttta ccagcggcac ggaagtgacc gatgttagtc atgcaagaac cgataaatac
    15901 ttcgtcgatc ttctcgccct gtaccgcaga cagcggacgt gcgtcatccg gatcgttcgg
    15961 agcacacagg attggctctt taatctccgc cagatcgatg tcgatcactg ccgcgtattc
    16021 cgcatctgca tcggcttcca gcagctcagg attcgccagc catttttcca tgccctgaat
    16081 acggcgttcc agggtacgac gatcgccgta accttccgcg atcatccact tcagcaggac
    16141 gatgttggag ctcaggtatt cgatgatcgg ttctttgttc agcttgatag tacaaccagc
    16201 ggcagaacgc tcggcggacg catcggttag ctcaaaggcc tgctcaactt tcagatccgg
    16261 cagaccttca atttccagaa tgcgaccaga gaagatgttt ttcttgcctt tcttctcaac
    16321 ggtcagcaga ccttgtttga tcgcatacag cggaatggcg tgtaccagat cgcgcagggt
    16381 gatgcccggc tgcattttgc ctttgaagcg caccagaacg gattccggca tatccagcgg
    16441 cattacgcca gttgcggcag caaacgccac cagaccagaa cccgccggga aagagatacc
    16501 gatcgggaaa cgggtatggg agtcaccacc ggtaccgacg gtatccggca gcagcatacg
    16561 gttcagccag gagtgaatga cgccgtcacc cggacgcagc gacacaccgc cacggttcat
    16621 aatgaagtcc ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat acgccgcggt
    16681 gtggcagaaa gactgcatca ccaggtcagc cgagaagccc aggcacgcca ggtctttcag
    16741 ttcatcacgg gtcatcgggc cggtggtgtc ctgagagcct acagaagtca ttttcggttc
    16801 gcagtacgcg cccggacgaa tgcctttcac gccacaggca cggcctacca ttttctgcgc
    16861 cagcgagaag ccgcgatcgc tctcagcgac atctttcgcc tgacggaaca catcactgtg
    16921 cggcagacca agtgcttcac gcgctttggt ggtcaggcca cgcccgataa tcagcggaat
    16981 acggccgcca gcacgcactt catcaatcag cacgtcggtt ttcagttcga aggtcgccag
    17041 cagttcgcct gtttcgtggt tacgcacttc acctttgtac gggtaaacgt caatcacgtc
    17101 gcccatgttc aggttagaga cgctgacttc gattggcagt gcacccgcgt cttccatcgt
    17161 gttaaagaag atgggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg
    17221 cacatgtgga atatcatcgc ccataaacca cagaacggag ttcgtggcgg atttacgcga
    17281 tgaacccgta cccacaacgt caccgacgta cgccagcggg aaacctttct gttgcagagc
    17341 ttcaatctgt ttgatcgggc caacaacacc tggctggtct ggctcaatac cttcacgggc
    17401 gtttttcagc atcgccagcg cgtgcagtgg gatatccggg cgtgaccacg catccggtgc
    17461 cggagaaagg tcatcggtgt tggtttcgcc agtgacttta aagacggtaa cggtcagttt
    17521 ttcagccagc gccgggcgat tcaggaacca ttcggcatcc gcccaggact gcattacctg
    17581 cttcgcatat tcgttgcctg ctttcgcttt ctcttctacg tcatagaagt tatcgaacat
    17641 cagcagcgtg tgagaaagtg ctttggcagc aataggtgcc agtttggcat catccagcgc
    17701 gtcgatcagc ggatgaatgt tgtaaccacc ctgcatggtg cccagcagtt cgatagcttt
    17761 ttccggagtc agcagagggg atttagcttc gcctttagcg atagcagcca ggaagcctgc
    17821 ttttacatag gcggcttcat cgacgcctgg gggaacacgg ttggttaaca gatctaacag
    17881 gaattcttct tcgcccgcgg gcgggttttt cagcagctct acaagcgcgg ccatttggtt
    17941 tgcatccagg ggtttgggcg caatcccctc agcggcacgc tcagctacgt gcttacggta
    18001 ttcttctagc acgacggttc tcctcgctct cattgtcata gtgcggcagg cgattctctt
    18061 cacgctcctg tgagacagca gtttgtaggg taaatgccca gtaccgcaac gggcagaata
    18121 gcaggatttt gacgacgtgt taatctgttt acaaaaaagc aacataaaaa agtggctgaa
    18181 tcgttaagga tggtctagag attgtttccc tccgtaaaaa cttcgttaat tcgcatggtg
    18241 ataatcacct ttctcaacga atcccaagat tagtcaaaat ttaaactacc gcctctttat
    18301 actcggattc acagcacctg cgggtggcag ttcgccgacc attgcgattt ccttgagatc
    18361 cgaattatga aaatgacttt gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc
    18421 gggctttgtg ctgcctctgc cgggctatat ataaaaagcc gcacagtgga agcgcctgta
    18481 gaaccgcaat cgacacaact ggctgtgtct gacgctgccg ctgttacgct tcctgcaacg
    18541 gtttccgcac ctcccgtaat acccgcagtc gtcaaatccg cattcagcac tgcacaaata
    18601 gatcaatggg tcgcacccgt cgcgctgtat cccgacgccc tactttcgca ggtgctgatg
    18661 gcatcaacct atccgacaaa cgttgctcaa gcagtgcaat ggtcgcacga taatccactt
    18721 aaacaaggcg atgctgctat tcaggcggta tctgaccagc cgtgggacgc cagcgttaaa
    18781 tcactggtgg cctttccaca attgatggca ttgatgggcg aaaacccgca atgggtgcaa
    18841 aacctgggcg atgcttttct ggcgcagccg caggacgtga tggactcggt acagcgattg
    18901 cggcaactgg cgcaacaaac cggctcgctg aagtcatcaa ccgaacagaa agttattacc
    18961 acaacgaaga aagctgtacc ggtaaaacag acagtcacgg cacccgtcat accatccaat
    19021 accgttttaa ctgccaaccc cgtcattaca gagccggcaa caaccgtcat ttccattgag
    19081 cccgccaatc ctgatgtggt ctatattccc aactacaacc caaccgtggt ttacgggaac
    19141 tgggccaata ctgcgtatcc gccggtttat ctgccaccac cagccggaga accgtttgtt
    19201 gacagctttg tgcgcggatt cggctatagc atgggcgttg ctaccacgta cgcactattc
    19261 agcagcatcg actgagatga cgacgatcat gaccatcatc atcatgacga tgatgattat
    19321 catcaccacg atggcggtca tcgtgacagt aatggctggc aacacaacgg cgacaacatc
    19381 aatatcgacg tcaacaattt caaccgtatc accggtgagc atcttactga taagaatatg
    19441 gcatggcggc acaatccaaa ctaccgtaat ggtgtgccct atcatgatca ggatatggca
    19501 aagcggtttc atcaaaccga tgtcaacggc ggaatgagtg ccacgcagct acctgctcca
    19561 acacgcgaca gccagcgtca ggcggcagca agtcagtttc agcaacgaac acacgccgcc
    19621 cccgtcatta cacgagatac ccaacgtcag gcagcggcac agcggtttaa tgaagctgaa
    19681 cactatggga gctatgacga cttccgcgac ttcagccgtc gccaaccact gacccagcag
    19741 caaaaggacg ccgctcgtca gcgttatcag tcggcctctc ctgagcagcg ccaggcagtc
    19801 cgcgagaaaa tgcagactaa cccgcagatc cagcagcgaa gggaggcggc gcgccagcgt
    19861 attcagtccg cctcgcctga gcagcgccag gcggttcgcg agaaaatgca gactaacccg
    19921 cagaaccagc agcgaagaga ggcagcgcgt gagcgtattc agtctgcaac tcccgaacag
    19981 cgccaggtgt ttaaggaaaa agtacagcag cgcccactga accaacagca acgtgataac
    20041 gcccgccagc gtatacaatc ggcatcacct gaacaacgtc aggtttttcg ggagaaagtt
    20101 caggagagcc gcccacaacg tctaaacgac agtaaccata ctgtcaggct gaataacgag
    20161 caacggtcag cagtacgcga acgtctctct gaacgtggag caagaagact ggaaaggtaa
    20221 attgcagacg taaaaaaagc ggcgtggtta gccgcttttt taattgccgg atgttccggc
    20281 aaacgaaaaa ttacttcttc ttcgctttcg ggttcggcag gtcggtaatg ctaccttcga
    20341 acacttctgc cgccaggccc acagactcgt gcagagtcgg gtgcgcgtgg atggtcagtg
    20401 cgatgtcttc agcatcacaa cccatttcga ttgccaggcc gatttcaccc agcagctcgc
    20461 cgccgttggt accaacaatc gcaccaccga tcacacggtg agattctttg tcgaaaatca
    20521 gcttggtcat accgtctgcg cagtcagaag cgatagcacg accagaagca gcccacggga
    20581 aggtggcggt ttcatagctg atgcctttct ctttcgcttc tttctcagtc agacctaccc
    20641 atgcaacttc tggttcggta taggcgatgg acgggataac tttcggatcg aagtagtgtt
    20701 tcttaccggc gataacttca gcggcaacgt gaccttcgtg aacacctttg tgtgccagca
    20761 tcggctgacc gacgatatcg ccgatagcaa agatgtgcgg tacgttggta cgcagctgtt
    20821 tgtcaacgcg gatgaaacca cggtcgtcaa cttccacgcc agctttgcct gcgtcgaggt
    20881 ttttaccgtt cggcacacga ccaatcgcta ccagcacggc gtcgtaacgc tgcggttcag
    20941 ctggtgcttt tttgccttcc atcgtcacat aaataccgtc ttctttcgct tcaacggcgg
    21001 taactttggt ttccagcatc aggttgaatt tcttgctgat acgcttggtg aagactttaa
    21061 cgatgtcttt gtcagctgcc gggataacct ggtcgaacat ttcaaccacg tcaatctgtg
    21121 aacccagcgc gtggtaaacg gtgcccattt ccagaccgat gataccgcca cccattacca
    21181 gcaggcgttc tggtacttct ttcagttcca gcgcgtcagt ggagtcccag atacgcggat
    21241 cttcatgcgg aataaacggc agttggatcg ggcgagaacc cgctgcaatg atcgcattgt
    21301 cgaagttgat cacggttttg ccgttctcac cttcaacttc cagggtgtta gccccggtga
    21361 atttacccag accgttgacc actttgactt tgcggccttt cgccatacca gccagaccac
    21421 cggtcagctg attgatcact ttctctttcc aggtacgaat cttgtcaata tcggttttcg
    21481 gttcgccgaa gacgataccg tgttcagcca gcgctttggc ttcttcgata acttttgcta
    21541 cgtgcagcag tgctttagaa gggatacagc cgacgttcag gcaaacaccg ccaagggtgt
    21601 tgtaacgttc tacgattacg gtttccagac ctaaatcagc gcaacggaag gcagcggagt
    21661 aacctgcggg gcctgcccca agtaccacga cctgagtttt gatttcagta ctcatcatga
    21721 cctctatata tttatctccg gcggtcatac ccgtcgtctt tcaggccgca gcggcgttag
    21781 cttcacgccc tcaccccagt cacttactta tgtaagctcc tggggattct ggcgcttgct
    21841 accttgctgc acccagaaat ccatagggta tgtgaagtaa taccctaacc accaccgggt
    21901 cgttctatcc gtcggtattt tacaaaattg ttaacaattt ttaaacaaca aacggcaacc
    21961 gatttgtcta ttcgctaata acctcaatac attcatgaga ttaccagaaa aaagccggcc
    22021 gttgggccgg ctcttttact tacatcacca gacggcgaat gtcagacagc gtgttgttaa
    22081 tgatggtaat gaaacgggca ccatcagcac cgtcgatcac gcggtggtcg aaggagagag
    22141 aaatcggcag catcagacgc ggcacgaact ctttaccatt ccacaccggc tccatcgcgg
    22201 acttggaaac gccgaggata gccacttccg gcgcgttcac aatcggcgcg aagtgggtag
    22261 tacccaggcc gccgatgctg gagatggtga agcaaccgcc ctgcatttcg cccgcagtca
    22321 gcttaccgtc acgcgctttc ttagaaatag tcatcagctc gcgagacagc tcgatgatgc
    22381 ctttcttgtt gacgtctttg aataccggaa caaccagacc gttcggggta tccaccgcca
    22441 caccgatgtt gatgtatttc ttcagggtca gacgctgacc gtcttccgac agcgaactat
    22501 tgaagcgagg catctgctca agagctgcag caacggcttt catgatgaag acaaccgggg
    22561 tgatcttcac atccagctta cgtttcgccg cttcttcgtt ctgctgttta cggaacgctt
    22621 ccagctcggt gatatcggtt ttgtcgaagt gggtaacatg cgggatcatc acccagttac
    22681 ggctcaggtt agcaccagaa attttctgga tacggcccag ttccacttct tcgatttcgc
    22741 caaacttgct gaagtccacc ttcggccacg gcagcatgcc agggatacca ccgccagtcg
    22801 ccgccggagc tgcttctgca cgtttgatag cttctttcac gtaagcctga acgtcttcgc
    22861 gcaggatacg acctttacgg ccagtgccct tcactttcgc caggttaacg ccaaactcgc
    22921 gtgccagacg gcggatcagc ggggtcgcgt gaacgtaagc gtcgttttca gcaaattcag
    22981 atttgccttc cgctttcgca gccggagctt cagcttttgc tgccggagcc ggcgctgccg
    23041 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca
    23101 gcgaaccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    23161 acggagccgg aacttccata gaagctttat cgccttctac ggtaatcagt gactgttcag
    23221 cggcaacttt gtcgcccact ttcaccatca cttcggtcac ttcaacttcg tcaccgccga
    23281 tatccggaac gttaacttct ttcacgccag ctgctggtgc aggggtcgct gccggagtcg
    23341 cttcctgttt agccgccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    23401 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtacctg
    23461 caaacggagc cggaacttcc atagaagcct tgtcgccttc tacggtgatc agcgactgtt
    23521 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    23581 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc gggtgctgct gccggagctg
    23641 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    23701 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    23761 cgatacccgc ctgcggagat ggaacttcca tagaggcttt gtcgccttct acggtgatca
    23821 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    23881 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc
    23941 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    24001 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    24061 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    24121 agtacccagt acgcgatagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    24181 tttcatatag tcggtagatg ccactgccgg cgcgtcgttc atcacctgag cgatatacgg
    24241 aacgcgcgga gtttccagcg gatgcagcat gttccagcgt tcacaatcct gaccatcacg
    24301 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    24361 caggatctct gccgcttcac ggacgtgacg caggatggaa ccggagccca gcagctgaac
    24421 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    24481 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtagtga tgtagtagta
    24541 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcataa tgacagcaac
    24601 ttcgtaagcg taagccgggt cgtaagagat acagttcggg atagtcagcg actgaatgtg
    24661 gctgtgacca tcttcgtgct gcagaccttc accgttcagg gtggtacgac cggaagtacc
    24721 gccgatcagg aagccacgcg cttgctggtc tccagccgcc cagcacagat cgccgatacg
    24781 ctggaagccg aacatcgagt aatagatgta gaatgggatc atcggcagat tgttcgtgct
    24841 gtaagaggtc gccgctgcca gccaggaaca ccctgcgccc agctcgttga tcccttcctg
    24901 cagaatctga cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    24961 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    25021 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    25081 catcacgttc agagcacgaa cgaaagcgat agtggtagag agctctttgc tctgctcttc
    25141 cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    25201 acggcttggc agataaccgt gcagtttctg acgctgagcg tgcagatagg tatgctcttc
    25261 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    25321 attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    25381 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggtat gagcaaggat
    25441 tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    25501 atcgtgacca ccacggttca gtgcccagat ctgctcgtca gtccagtctg caaccagtgc
    25561 tgcggtttca ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt
    25621 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    25681 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    25741 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    25801 gtcaagacgc tgcaggttac agttgataac gaaaaccagg ttatccagtt tttcacgggt
    25861 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaacgc
    25921 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    25981 agcctggtaa atagcaccaa tcggacccag acccatagat acggtcggga actgccagaa
    26041 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    26101 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacggg cgtaaacgcc
    26161 cggggagatg tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg
    26221 gaagaagtgg ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatgtg
    26281 gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc gtcatgatgg cgttccagcg
    26341 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    26401 aacggggatg gtgttgatgt agttgctgat acctgtgcct gcggctacgt ttacaccgcc
    26461 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    26521 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    26581 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    26641 cctgtcccat tgaactctcg ccagaaagct caataagaca ggttctacgt ttagttaccg
    26701 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag aaaaatcact
    26761 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacggcttc
    26821 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    26881 ccggcttacc gaccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    26941 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    27001 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttcg gtgacggcaa
    27061 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagct
    27121 ctatggcgtg gtggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    27181 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgatt
    27241 caggatggtc ggagagcagc tccaccagcg gatcgctgaa gctttgccat aggctgctct
    27301 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    27361 tcgcctcacg caaggaggga cgggagacgt cgaactgttt tgccagttcg cgttccggtg
    27421 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    27481 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    27541 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    27601 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    27661 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa
    27721 agggattttt taacgattat taaactataa aaatccgaat tgaaccgatt cacttaccaa
    27781 ttttttgatt tttaagtcaa taaaaacgaa tttaaattca ctctgcatat tgagaggggt
    27841 tgaggctgag ctttacaaac ggtttctttt taagcaactc atcttcaacc atgcataaag
    27901 cgggtgcatt cgctgccgca taccattatt cttgatctga cggaagtctt tttgtaacaa
    27961 ttcaaacctc cttgatgtaa acaaatcaat acaacaaacg gaattgcaaa cttacacacg
    28021 catcactgcg tagatcaaaa aaacaatcac cgcacgaggt ttcatgatgg aaggtcaaca
    28081 gcacggcgag cagctaaagc gcggccttaa aaaccgccat attcagctta tcgcgctggg
    28141 tggcgcgata gggacagggc tattcctagg tagcgcctcc gtaatacagt ccgcagggcc
    28201 agggattatc ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct
    28261 gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata
    28321 ctggggcagt tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt
    28381 tgccatggca gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc
    28441 aacctgggtt tctgccgccg tattctttgt ggtgattaac gccatcaacc tgaccaacgt
    28501 taaagtgttt ggcgagatgg agttctggtt tgccattatc aaagttattg cggtagtagc
    28561 gatgatcatc ttcggcggct ggctgctgtt cagtggcaac ggcggcccgc aggcgagcgt
    28621 tagcaacctg tgggatcaag gtggtttcct gccgcacggc ttcaccggac tggtgatgat
    28681 gatggcgatt atcatgttct cgttcggtgg tctggaactg gtggggatca ccgcagcaga
    28741 agctgataac ccggagcaaa gtatcccgaa agcaactaac caggttatct accgcatcct
    28801 gattttctat attggttcgt tagccgttct gctctcactg atgccgtgga cccgcgttac
    28861 cgccgatacc agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa
    28921 tgcgctgaac atcgtggtac tgactgcggc gctctccgtg tacaacagct gcgtatattg
    28981 caacagccgt atgctgtttg gtctggcaca acaggggaac gcgccaaaag cgctggcgtc
    29041 tgtcgataaa cgcggcgtac cggttaacac cattctggtg tctgcgctgg ttacagcatt
    29101 gtgcgtattg attaactatc ttgctccgga atccgcattt ggcctgttaa tggcactggt
    29161 ggtatccgca ctggtgatca actgggcgat gatcagtctg gcgcatatga agttccgtcg
    29221 cgccaagcag gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa
    29281 ctggatctgc ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat
    29341 ggcgatttcg gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt
    29401 taaagagaaa accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtac
    29461 agggtgaggg caataatctc tacaccaacg ccccgtaaat cgtcagtagc gcaatcacca
    29521 ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct
    29581 tatcgacatg cggttcacgc gccagagaga actgcgccag acgcgttaat acctgatatt
    29641 gcgaagtatg gaaatcaccc agcgaagcaa accaggccgg taacgctttc tcaccatgac
    29701 cgatcaaggc atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca
    29761 ctgcatcaat gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt
    29821 gccatgcacg caagaacgca taccccatca gcgtaacggg tccccaggtt ccccccacaa
    29881 tcagccagaa cagcggtgca agataaaaac gaaagttaat ccacagcaat gcattttgca
    29941 gctcacgcaa atactcacgt tcgtcgcagc ctgccgggac gccgtgaatc atggtgagtt
    30001 cgccagccat cgtggcacgg gcatggctat cattacgtga agcagctgtc agataagcat
    30061 gataatgaag acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca
    30121 gcagcgtggg aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacac
    30181 ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa
    30241 agaacgcttc aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga
    30301 aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg
    30361 aaaccgtgcc caatcaaatg caggaccggg atcggttttc cgatccggcg caatatcaca
    30421 atggcccgtc atgttattag cgatatcggg atagcgatct atcagtgccc gcgtaaccgc
    30481 cgcaagctgt tgatactgcg catcggtata cgccagagta tcggtgcctt caagctcaat
    30541 cccaatagaa aaatcattgc agcgttcacg cccctgatac tgagagactc ccgcatgcca
    30601 ggcacgttta tcgaaaggaa catactggac tatttcacca tcacggcgaa tcaaacagtg
    30661 agcggagacg cgcaaatggg cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt
    30721 tccagtgaat aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt
    30781 gtgcaccacc agcagggtgg gtgtttcgtc atccaggcgg caatcgtaat gtggtgaggg
    30841 aacgcggcgc gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg
    30901 tggtgctaat acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg
    30961 agttttaaca tgccgcctcg ccgctacaac cctgacaccc gacgtgacga gctgctggaa
    31021 cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga
    31081 acagtcgatg ccaacaatga cattacggca aaacttttac cggaaaattc tcgctctcat
    31141 gccacggtga tcacccgcga gaatggcgta ttttgtggca aacgctgggt tgaagaggtg
    31201 tttattcaac tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc
    31261 atcaacgcca atcaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc
    31321 gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac
    31381 tatgtcgaat tactggaagg caccaacacg cagttgttgg atacgcgcaa aaccttacct
    31441 ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg
    31501 gggctttctg atgccttcct gatcaaagaa aaccatatta ttgtctccgg ctcagtgcgc
    31561 caggcggtcg aaaaagcgtc ctggctgcat ccggatgcgc cagtagaagt cgaagtagag
    31621 aatctggaag aacttgatga ggccctgaaa gccggagccg atatcatcat gctggataac
    31681 ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa
    31741 gtgtctggca acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt
    31801 atctccgtcg gtgcgctaac taaacacgta caagcactcg acctttcaat gcgttttcgc
    31861 taattgctga cagatttatc cgctccatcg cgggcggata atctcccctt ccccaacttt
    31921 cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac gaaataaaaa acctcggaat
    31981 ctgctttccc gtttcgtttt ttgcccctcg cctgtgctga tggcgtttgc caaagtagca
    32041 ccaaccaaat caaggagcga aacagatgga caagcaacgc ggttttacac ttatcgaact
    32101 gatggtggtt attggcatca ttgccatttt aagctccatt ggtattcccg cttatcaaaa
    32161 ctacctgcgc aaagccgcac tcaccgacat gctacaaacc tttgtgcctt accgtaccgc
    32221 cgtagagttg tgcgcgctgg aacatggtgg attagatacc tgcgacggtg gcagcaatgg
    32281 cattccctcg cctaccacca cccgctatgt ttcagccatg agtgtggcaa agggcgtggt
    32341 gtcgctgacc gggcaagaaa gtctcaatgg gctaagcgtc gtcatgacgc caggttggga
    32401 taacgcaaac ggcgtcaccg gctgggcgcg caactgcaat attcaaagtg acagcgcatt
    32461 gcagcaagcc tgcgaagatg tcttccgctt tgatgacgcc aactaaggag cggcaatgaa
    32521 tattccacag ctcacggccc tgtgcctgcg ttatcaggga gtcttgctgg atgccagcga
    32581 agaagtggtt catgttgcgg tggtcgatgc cccctcacat gagttgctgg acgcattgca
    32641 tttcgctacc accaaacgta ttgagatcac ctgctggacg cgccaacaaa tggaaggtca
    32701 cgccagtcgc acacaacaga cattacccgt agctgttcag gagaagcatc agcccaaagc
    32761 agagttgctg actcgaacgt tacaatctgc gctggaacaa cgcgcgtctg atattcatat
    32821 cgaaccagcg gacaatgcct accgcatccg cttgcgtatc gacggcgtat tgcatccttt
    32881 accggatgtt tcaccggatg ccggagtcgc attaacagcc agattaaaag tgctgggaaa
    32941 cctggatatt gcggaacatc gcctgccgca ggacgggcaa ttcactgtcg aactggcagg
    33001 aaacaccgtc tcatttcgta ttgcgacctt accatgtcgg ggtggtgaaa aggtggtatt
    33061 aaggttgtta cagcaggtga gccaggcact ggatgttaac acgctgggaa tgcagccgtt
    33121 acaactggcg gactttgctc atgccttgca acaaccacag ggactggtgc tggtaactgg
    33181 ccctacaggc agtggcaaaa cggtcacctg gtgttgtcca cgctacacac caattccacc
    33241 actgaaacgc tggtacgttt acagcaaatg ggggtcgccc gctggatgct atcatcggcg
    33301 cttacgctgg taatagccca gcgtctggta cgtaaacttt gcccacattg tcgccgacag
    33361 caaggggagc ccatccatat tccagacact gtatggccgt cgccgctgcc ccactggcag
    33421 gcacccggtt gtgtacattg ctaccacggt ttttatggtc gtaccgcctt gtttgaagtc
    33481 ctgcccatta caccagccat acgccagctt atttccgcta atactgaagt tgaatcgctg
    33541 gaaacgcacg cccgacaggc gggtatgcgt acgctttttg aaaacggctg cctggccgtg
    33601 gagcaaggct taaccacctt tgaagagtta atccgcgtac tggggatgcc gcatggcaag
    33661 taagcaactc tggcgctggc atggcataac cggcgacggc aatgcgcaag atgggatgct
    33721 atgggcagag agccgtactt tactgcttat ggcactacag caacagatgg ttaccccact
    33781 aagcctgaag cgaatcgcca tcaattctgc gcagtggcga ggagataaaa gcgcggaagt
    33841 cattcatcaa ctggcgacgc tacttaaagc cgggttaacg ctttctgaag ggctggctct
    33901 gctggcggaa cagcatccca gtaagcaatg gcaagcgttg ctgcaatcgc tggcgcacga
    33961 tctcgaacag ggcattgctt tttccaatgc cttattaccc tggtcagagg catttccgcc
    34021 gctctatcag gcgatgatcc gcacgggtga actgaccggt aagctggatg aatgctgctt
    34081 tgaactggcg cgtcagcaaa aatcccagcg tcagttgacc gacaaagtga aatcagcgtt
    34141 gcgttatccc atcatcattt tagcgatggc aatcatggtg gttgtggcaa tgctgcattt
    34201 tgttctgccg gagtttgccg ctatctataa gaccttcaac accccactac cggcactaac
    34261 gcaggggatc atgacgctgg cagactttag tggcgaatgg ggctggctgc tggtgttgtt
    34321 cggctttctg ctggcgatag ccaataagtt gctgatgcgc cgcccgacct ggcttatcgc
    34381 gcggcagaaa ttgctgttac gcatcccgat tatgggttca ctgatgaggg gacaaaaact
    34441 cacgcagatc tttacgattc tggcgctgac acaaagtgca ggcattactt ttttacaggg
    34501 cgtagagagc gtcagagaaa caatgcgctg cccgtactgg gtgcaacttc tgacacaaat
    34561 ccagcacgat atcagtaacg gtcatcccat ctggctggcg ctaaaaaatg ccggtgagtt
    34621 tagcccgctc tgtttgcaat tagtgagaac aggagaggca tccggctcgc tggacctcat
    34681 gttagacaac ctcgcccatc atcatcggga taacacaatg gcgctggcgg ataacctcgc
    34741 agccttactg gaaccggcgt tgctgatcat aacgggagga attatcggta cgctggtggt
    34801 ggcaatgtat ctgccaattt tccatttagg cgatgcgatg agtgggatgg gataacgctg
    34861 gcgtgttgct ccacgccagc gttgggagat tacaggttgt tgaagatgcg gttttcttgt
    34921 tccagcacac gaataaacgt ggtgcgcttg gtcagctctt tcaggcgtga agccccaacg
    34981 tatgtacaag ctgaacgcag gccgcccaaa atatcacgcg cggtattttc aacctggcct
    35041 cgcagcggca gcttaacggt tttaccttct gctgcgcggt attccgcaac gccgccaacg
    35101 tgacgtttca tcgcagactc ggagctcatg ccgtagaaca gcataaattt ctcgccgttc
    35161 tcctcaacga tgcgaccgcc gctctcttcg tggcccgcca gcatgccgcc aagcatgacg
    35221 aaatcggcac cgccgccaaa ggctttcgcc acatcgcccg gcgtggtaca gccaccgtcg
    35281 ctgacgatca ttccgcccag accgtgcgcc gcatcggcac attcgattac cgcagaaagc
    35341 tgcggataac cgacgcctgt tttgacgcga gttgtacaaa cagaacctgg gccaatgcca
    35401 actttaacga tatcagcacc ggagaggata agctcctcac acatttcacc agtcactacg
    35461 ttgccagcac aaatggtttt ggtcggccac gcttcacgcg ctttcgcaac gaactgcacg
    35521 aagtgttcgg aataaccatt cgccacatca atacaaacga agtttaatgc cgggttcagg
    35581 tcgagaatct gtttagtttt ttcgaaatcc gcatcagacg tgccggtaga aaccatcaca
    35641 tgtttcagca catcagcgga agaattgttg ataaacgctt gccactcttc gacagaaaag
    35701 tgtttatgca cagcagtcag aatatcaaaa gaagccagcg cagaggccat agaaaatgtg
    35761 cctacggtgt ccatatttgc ggcgataatc ggcacgccgg accagctctg acctgaatgt
    35821 ttgaaggtga attgacgttc cagttcaaca tcggaacggc ttttaagagt ggagcgttta
    35881 gggcggatga gaacgtcttt aaaacctaac ttcagatctt cttcaatacg catgtgcgga
    35941 ttcctggggt taatggctat aagggtaaga gcgcaactcc agtgacgcta tcatacgcac
    36001 taatcaatgc ggcgcaagac tgcgaaattg tcttttttta cgctacaatc ccataaattt
    36061 acctggtaaa tagtgagcga acgtttcatc aatttcactt tcgcgcgctc atttttaacg
    36121 ttttgataat catgatgaat tcccgggaat ataaattatg gggtatatag ttgccttaac
    36181 gggaggcatt ggcagtggta agagtaccgt tgccaatgcg tttgctgatc tcggaattaa
    36241 cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa ccaggtgcac ctgcgctaca
    36301 tgccattgct gatcactttg gcgaaaacat gattgctgct gatggaacat tgcagcgccg
    36361 ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa aactggctta acgacctgct
    36421 gcatccgctg attcaacaag agacgcaaca ccagatccag caagcaactt ccccctatgt
    36481 actgtgggtt gtgccattgc tggtagaaaa ctcactgtat aaaaaagcga atcgagtgct
    36541 ggtggtggat gtcagcccag aaacgcaact taagcgcacc atgcagcgcg atgatgtaac
    36601 tcgcgaacat gtcgaacaaa tccttgctgc tcaggcaacg cgcgaagccc gccttgccgt
    36661 ggcagatgac gtcattgata ataacggcgc accggatgct atcgcatcgg atgttgcccg
    36721 cctgcacgca cactatttgc agcttgcgtc gcagtttgtc tcacaggaaa aaccgtaatg
    36781 cagacccagg tcctttttga acatccacta aatgaaaaaa tgcgtacatg gctgcgcatt
    36841 gagtttttga ttcagcaact caccgttaat ttacccatcg ttgaccacgc tggcgcgctg
    36901 catttcttcc gtaatgtcag tgaattactg gatgttttcg agcgcggcga agtccgcact
    36961 gagctgttga aagaacttaa ccggcagcaa cgtaaactcc agacctggat tggcgtgcct
    37021 ggcgtggacc agagccgtat tgaagcatta attcagcagt taaaagcggc ggtgagcgta
    37081 ttaatttccg cgccgcgtat cgggcaattt ctgcgtgaag atcgtttgat tgctctggtg
    37141 cgtcagcgac tgagcatccc aggcggctgt tgcagctttg atttacctac attgcacatt
    37201 tggctgcatc taccccaggc gcagcgcgac agccaggtag aaacctggat tgccagcctg
    37261 aacccgctca cccaggcact taccatggtg ctggatttaa ttcgccagtc ggcccccttc
    37321 cgtaaacaaa ccagcctgaa tggtttttat caggataacg gtggcgatgc cgacttgctg
    37381 cgcctgaatc tgtcgctcga ttcacagctt tatccgcaaa tttccggaca taagagccgt
    37441 tttgccattc gttttatgcc gctggacact gaaaacggac aggtaccgga acgtctggat
    37501 ttcgaactgg cctgttgcta aggagtaaaa atgtcagaaa ctattacggt gaattgccca
    37561 acctgcggga aaacggtggt gtggggtgaa atcagcccgt ttcggccatt ttgctccaaa
    37621 cgttgtcagc tgatcgacct cggagaatgg gctgctgaag aaaaacgaat ccccagcagt
    37681 ggcgatcttt ccgaaagcga tgactggagc gaagagccaa agcagtgaca tttgtctgat
    37741 gcctcacgta ggcctgataa gacgcggaca gcgtcgcatc aggcatcttg tgcaaatgtc
    37801 ggatgcggcg tgaacgcctt atccgaccta cagacgctta agcttcgcaa ttaccggttc
    37861 attggctggc ggaaaatcat cggcattaag accgaccagc gacatccact caccgggttg
    37921 cccttcttta ccccacggcc ccccttccca actttcgacc agccagaacc acagagttat
    37981 atgcctgtcc gggaattcat attccagttt ttcaaatagc gaaaaatgtt gtggggtaat
    38041 cccgacctct tcctgaagtt cacgcaccac cgcctgttcc ggcgtttcgc ccatttcaat
    38101 tttaccgccg ggaaactcca gtttattcgc catgtgcgca tctgctgcgc gacgtgttat
    38161 aaagatttca ttgttctcgt tgcgaataat acctaccgca atttgcagct ttttcattgt
    38221 cttaaaccta taaaaaaggc gcagaatcct gcgcctttta cttcaactgt tagcttttat
    38281 tgcaggcgac catggcactg cttgtatttt ttaccagaac cgcacgggca aggatcgtta
    38341 cgtcctactt tgcgttcacc ggtttgcgcc gccagtgcag ctgcggctgc agagtcgtca
    38401 tcctgatggc taagctgctg catttgtgct aaacgctcgg cttccatacg acgctgttgt
    38461 tccagctcct caacctcttc aggcatacgt acctgaactt tgctcagcgt actgataact
    38521 tcatatttca acgactccag catcgctgca aacatggaga acgattcacg tttgtattcc
    38581 tgcttcggat ctttctgtgc atagccacgc aggtggatac cctgacgcag atagtccatc
    38641 gctgccaggt gctctttcca cagggagtca agcgtttgca gcatgacgcc tttctcgaag
    38701 tgacgcatca tctcagcacc aaccacttct tctttacgct gatacacttc gatggactgc
    38761 gccagaatgc gctcacgcag cgtctcttca tgcagttctg gttctttatc cagccactcg
    38821 gtaattggta aatcgaggtc gaaatcgttc ttcagacgtt cctgcagccc cggaatatcc
    38881 cacatttctt ccagcgactg tggcggaatg taggcatcaa tggtggcttt gaacacatct
    38941 tcacgaatgc tgttgatggt ttcgctcaca tcgctgacat ccagcagttc gttacgctgg
    39001 gagtaaatgg cgcgacgctg atcgttagcc acgtcatcat attccagcag ttgcttacga
    39061 atgtcgaagt tacgactttc aactttacgc tgggcgttgg caatcgcttt agtcacccac
    39121 gggtgttcaa tggcttcgcc tggcttcata cccagtttac gcatcatgcc ggatactcgg
    39181 tcggaagcaa aaatacgcat cagcgcatct tccatcgaca ggtagaaacg ggaagaacca
    39241 gcatccccct gacgaccaga acgaccgcgc aactggttat cgatacgacg ggattcgtga
    39301 cgctcggtac cgatgatatg caggccacct gcttccagta ccgcatcgtg acgtacctgc
    39361 cagtcggctt taattttttc aatttgctct gcggtcggat tttccagcgc ggcaacttct
    39421 gcctgccagc tacctccgag cacaatatct gtaccacgac ccgccatgtt ggtcgcgata
    39481 gtcaccgcag ccggataacc tgcctgagca acaatcgccg cttcgttggc gtggaatttg
    39541 gcgttcagga cattgtgctt aataccggct ttggtcagtt cgtttgacac cagctccgat
    39601 ttttcgatgg agatagtacc caccagcacc ggctggcctt tcgcagtacg ttctttgata
    39661 tcttcaatga tcgcctgaat tttttccgct tcagtcatgt agaccaggtc cggcagatct
    39721 ttacgaatca ttggacggtt ggtcggaaca acgacggtat ccagtttgta gatggagcta
    39781 aattcgaaag cttcggtatc agcagtaccg gtcatccccg ccagtttttc atacagacgg
    39841 aagtagttct ggaaggtgat cgaagccagc gtttggtttt cgttctggat ctgcacacct
    39901 tctttcgctt ccacagcctg gtgcagacca tcggaccagc gtcggccctg catggtacga
    39961 ccggtgtgtt cgtcaacgat gataacttca ccatctttaa cgatgtagtc gacgtcacgg
    40021 gtaaacagcg catgagcgcg cagcgccgcc gttacgtggt gcatcagcat gatgttggcc
    40081 ggagagtaca gagactcccc ttcatccatg atgccctctt tcaccagcag ttcttcaatc
    40141 agcaccagac cacgttcggt caggttcacc tggcgagatt tttcgtccac cgagaagtgg
    40201 ccttcgccct ggaaggtttc ggagtcttct ttttcctgac ggatcaggtg cggaataatt
    40261 ttattcacgc gtttatacat ttccgagctg tcttctgccg ggccggaaat gatcagcggt
    40321 gtacgcgctt catcgatcag gatggagtcc acttcgtcca ccagcgcata gtgcagttta
    40381 cgctgtacac gttcttcagg gctgaacgcc atgttgtcgc gcaggtagtc aaagccgtat
    40441 tcgttgttcg taccgtaagt gatgtcagct gcgtaagctt cgcgctttgc cggtgctggc
    40501 atgcccggca ggttgatacc aacagtcagg ccaaggaatt caaacagcgg acggttgttt
    40561 tcggcgtcac gttgcgccag gtagtcgttg acggtaacta cgtgcacgcc tttaccggtt
    40621 agtgcgttca ggtaagcagg cagcgttgcg gtcagggttt taccttcacc ggtacgcatt
    40681 tcggcgatgc agcgttcgtt aagaaccata ccgccgagta actgaacgtc gaagtgacgc
    40741 ataccaaaga cgcgcttact tgcctcacgc accacggcga aagcttccgg gatcagattt
    40801 tccagcactt cgcctttttc cagacgcgca cgaaactctg cggttttccc tttcagttct
    40861 tcatcggaga gtttttccat ctccggttcc atggcattga tgatgttgac cactttgcgc
    40921 atccggcgca gggtgcgatc gttacgacta ccgaaaactt tagttaacaa tttgattagc
    40981 ataataaaat ctcaaacgcc ccgcgttacg gagttaataa aatgaagtaa aggtttattg
    41041 ttgttaggtg aggcgttgag ggccagcacg gatgccttgc gcctggctta tccagacggg
    41101 cgtgctgaat tttgcttgtg gggtaaaatg cgcataatca atgcgataac ccttttcaga
    41161 cggcgtgcct tcctgggtca gcagcgcgct gagcgtatcc agtaatgcaa gatgttgcgc
    41221 ctgaagaggc aaagattctt cagcaacggg cagtgtttgc ggtgccattg cgaaagaaag
    41281 atgacggatt accgtacgaa tagcatgttg atgccagtaa tcaacggaat agttcgaatt
    41341 cgggcggcgc gtgttcgctt ccagcaaggc caattgacca aagttaactt tggctgaagg
    41401 ctcgtggttg cgggttgtcg cttttgcggg cgcgtttggt tcggcggcgt tgctgagcgc
    41461 aggcaaacct aaactcgccg cgaccatccc taataagaga tgcggccaga agtagcgttt
    41521 accaaactgt cgccagcgcg tcagtattcc actcacgtta ttgccatccc gatcgaattc
    41581 aaacgtattt agcgcacaaa tcttatcatt aaagcctgaa gtccacagca cgaatgaaga
    41641 taagttgtgc gtaaaagtgc ttaagaaaac agcgttcgca ccgtttttta tagctcttat
    41701 aaaattcaaa aaaaaacgac tggttcacct ggccggagag agtgccagat ttgccagtcg
    41761 aattttatac gacagtataa atgtcgttat gccagtacag ctgaaggcgc tttgaaggcc
    41821 aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg tttcgccagg
    41881 acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt aaatgcacca
    41941 ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt gtgacgcaca
    42001 aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg
    42061 gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc gatatcacgc
    42121 atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc ggagaagttc
    42181 atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat ggtgaaatcc
    42241 agcgaaaaac cattgtacgg cttaaattca gcccacttat cgccatcttc gacacgaaca
    42301 gtctctttga tgcgaacaaa ttttttggcg cagttcaact cgtcgatacc ggcgtcaagc
    42361 agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg cgcgttaact
    42421 tcgataacaa tgttatcgat gcccaggccc gcgagagcag cattgaggtg ctctacggtt
    42481 gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc acgcacagat
    42541 ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata gatgaccccg
    42601 gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg taaaccgaca
    42661 cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa
    42721 attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca caaagagcct
    42781 cgaaacccaa attccagtca attcttaatc agcttgctta cgtaggaatg ctgggatatc
    42841 cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcactactt tagcaaccgg
    42901 cttctgctcc tgggtcagcg gagccatccc atgctgctgg tagcgatcca tcactggctg
    42961 ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt
    43021 cgcaacaacg gttacgcgca gctcgtcatt catatccggg tcaagagaag taccgataac
    43081 cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt cgaactcatc
    43141 cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg ccgcgcgcgc cagacaggtc
    43201 gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg cacggtcttc
    43261 accgctcgcc acgccagaac ccatcattgc gtagcccatt tcagacatta cggtgcgtac
    43321 gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcggcga taccttgcac
    43381 agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga taccgcggcc
    43441 cagaactttc agcagtttgt cgttcgggat agtgatcaga gagtccacat gcttggacag
    43501 ttcagtgatc ccctgctcgg cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt
    43561 agtgacgaca gcaacggtca ggatacccaa atcttttgcc acttcagcga cgactggtgc
    43621 tgcacctgta ccggtaccac cacccatacc cgcagcaata aagaccatgt ctgcaccttc
    43681 cagcgccgca cgcaatgcat cgcgatcctc atcagccgca ttgcggccaa cttctggatt
    43741 agcgccagcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc
    43801 tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac cttcaatacg
    43861 ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga tgactttaat
    43921 caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat ttgtgcctgt
    43981 cgcctgagtc cgtaatcatc gtcggcctca taaaaattaa aactcttttc gcagccaact
    44041 attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta cttcagcttc
    44101 accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg aataatacgg
    44161 ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac gcacttgcgt
    44221 atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc caccggttaa
    44281 tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt cctgcaactg
    44341 caatatctct tcgttgacca ggttgagcaa ctcggtatag cgcggctcga tcacctctgc
    44401 cagggtctga cgttgcagac tccgcggcgg acgaccgcct acgctcggca cttccacgct
    44461 ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa tcgcttcggc
    44521 gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat tgccagcata
    44581 aggaattacc tttgtgtggc gcaatgcccc accggtataa acggcgatat ccattgtacc
    44641 accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca ataccgaata
    44701 acttgatgcc agtccggcaa atatcagttg gtcaactttc agcccacaac gttcaaccgc
    44761 tttgacgatg tttttcgcca tatcgttgtg acatgtgatc aggtgcactt ttgcctgcat
    44821 ccgcacaccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa tcgcatactc
    44881 ttgcgggatc acatgcagca cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg
    44941 gacgacgttt tccacatctt cttgcgtcac ttcttcttca gaaataggca ccataccaat
    45001 ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag agatctgaca
    45061 atctgccatc aattctgcct ggtcaatggc gcgttgtacg cacttgacca cggattcgag
    45121 gtcgttcacc ccgcctttat ccataccacg tgacgggcag ctgcccacgc caatgatatt
    45181 gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg taccaatttc
    45241 cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga
    45301 ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc cgctccagag
    45361 tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa
    45421 accggataaa gttctacaaa gcgaaccaaa cgtttcatcg tatcgccccg gccaagattg
    45481 agcttaatat cgttattcag cgtcaactgc caggaacgcc gcgcggtcat cgccgcttcc
    45541 ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc ctgcaacact
    45601 tcattagcgc tgccttccgg gccatacagc attggaagca cctgcttgct ggtgcgatct
    45661 ggcggcacgc tgaaggtgtt tccttccgcg tctaccatgt gttgatcatt ccaccgcgca
    45721 atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt tctgacgctc
    45781 acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt gacatcctgg
    45841 gtcataaagg tacccggctc acccaatgcc aggatcgact gccggatatc gtcattacgc
    45901 gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg cgcatcttcc
    45961 atccagctca acacgaccca gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa
    46021 aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc ttcgctgttt
    46081 cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc gtactaccaa
    46141 ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accaggctgt ggctggtcat
    46201 acccggtgag gtattggctt ccagcagata aaactgtcca tcactgtcca gcataacgtc
    46261 aatacgtccc catcctttgc aacctaacgt cgtccatgct ttcagcacta atgcctgcaa
    46321 attggcctct tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct catcagagag
    46381 atacttcgcc tcataatcat agaaggttcc ggacggttga atacgtattg acggtaaaat
    46441 ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt caatcaatac
    46501 ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt ctgctactac
    46561 ttttgacatt cccacactgg aaccttcgcg gctcggctta acgataaccg gcaaacccag
    46621 agcagaaatt tctgctaact gcttatcgct caggcctttt tcaaactctg cgcgggttaa
    46681 cgctacccac ggcgcgaccg gtaaaccggc accttgccac agaagtttgc tgcgtagttt
    46741 atctattgaa agcgcagatg ccatcactcc gctgccggta taaggcaagc ccatcagctc
    46801 gagcatgccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga taaacacttt
    46861 ctgaaaaccc atcgacttca gttgcgtcac gtcgacttct ttcgggtcga caggatacgc
    46921 atcaataccg ccttcacgca gcccggctaa caccgctgcg ccagaattca gagaaacttc
    46981 ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta tcagtcatgt tgttcttcct
    47041 ccggagtttg cggcttcagt ttgatttcag ctaaagaacg ggcaattttt ccgatattac
    47101 cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc atctcggcta
    47161 cctgcgcagg gtccggcacc agaatgggat caattttccc acgtccacga attgtgcgac
    47221 acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac acttccagca
    47281 tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg tcgcgcgtac
    47341 gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag cctgcgcgcg
    47401 ccgctttaat ggtggcgtca acttccgtcg ggtggtggcc gtagtcatcg accagcattg
    47461 ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa tcaaaacggc
    47521 gaccagtccc ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca attccctctt
    47581 ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacggcct ggcgcattca
    47641 gggtgacgca catcggctct ttgtcctgac gcagcagcgt aaagtgcccc tgcgggccaa
    47701 tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccgtaa gttgtggtct
    47761 gacgtcccac acgcggtaac aattcgcgga tcaccggatc atcaacacac atcaccgcac
    47821 gaccgtaaaa aggcaggttg tgcagaaaat taataaaggt ctgttttaaa ttctcaaagt
    47881 cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc accatcggtt
    47941 gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac cgaccatgcc
    48001 ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag gttgggtcga
    48061 gccccgcttc tgcgtagatg ctggaaacca tcgcggtagt tgtcgttttg ccgtgcgttc
    48121 ctgcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg gcacgacgga
    48181 tcaccggaat acgcgcttca tgagctgcga caatttccgg gttgtcggca gaaatggcgc
    48241 tggaaacaac gaccacgctg gcatcacgta cgttttccgg gcgatggttg aaataaatcg
    48301 tcgcacccag attcagtaac tgctgcgtga ccggatttgg cgctaaatcg gaaccactga
    48361 tctgataacc ttcattggcc agaacttcgg caataccgcc cataccggca ccaccaatgc
    48421 cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc agttttgcca
    48481 attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat gcaaaaggca
    48541 tcgctacaat tacgcccggg cagcccggct cacttcattt gccactcgct cggtggcatc
    48601 cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc gcgaccaccc
    48661 ggccagggtg ttggcgacag catccacgct aagctgtggc tgctcgataa ttttggctgc
    48721 gcccgctttt tccagcggta gcgcattcca gtattgctgg cggtctttat gttgaaacgg
    48781 cacaaacaac gccggtagtc ctgccgcggc gatttcactc acagttaaag caccggagcg
    48841 gcaaacgacg acatccgccc acgcatacgc cgccgccata tcatcaataa attcagtcac
    48901 tttatgctgc ggttgccccg cttcggcata cgcctgttca acggattgtt gcgaaccttt
    48961 gccgctctga tgccagatag tgactgaatc acccagtttc gcagcaacct gcggcattgt
    49021 ctggttaaga atgcgtgcgc cctgggaacc acccactacc aacacacgaa ccggaccttc
    49081 acgtccagcc aaacgttgct gcggcaacgg cagcgccagc acatcggtac gcaccgggtt
    49141 acccaccact tccgcattag ggaaagcgcc tggaaacgcc tgcatcactt tggtagcaat
    49201 cttcgccagc catttattgg ttaagcccgc aataccgttt tgttcatgaa gtacaaccgg
    49261 aatgcctaac gaccacgcgg ccagaccacc tggacctgat acatagccgc ccataccgag
    49321 taccacgtca ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg cgttgaagat
    49381 acgcagcggc gcagctatca gtgcttttat accttttcca cgcaaaccag agatacgaat
    49441 gaaatcaatt tcgatgccat gttttggcac taagtccgct tccatacggt cggcagtccc
    49501 cagccagcga acttgccaac cctgagccat tagatagtgc gcaaccgcca gtcccgggaa
    49561 tacatgtcca ccggttccgc ctgccatcac cattaatcgc tttccttgac cactcatcgt
    49621 gaacctcgta caaacgcctg cgctttctcc agacgcgttt cataatcaat acgcaacagc
    49681 atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat tagcggcaat
    49741 gtcagacctt tggtcggtaa catccccgcc gccgcgccta cgttaaccag cgcctggaag
    49801 ctaaaccaga tgccaataga acaggcgaga aaaccggaaa aacggtggtc aatttctaat
    49861 gctttgcggc caatcgacat cgcacgaaaa gcgacgaaga ataccattaa aagtgctagt
    49921 accacaccga cataacctaa ttcttcgccg ataatggcga aaataaagtc agtgtgcgct
    49981 tccggcagat actccagttt ttgtaccgaa ttacctaaac cttgccccca aagttcgccg
    50041 cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg atcttcccac
    50101 gggttccaga atgcggtaac acggcggata cggtacggtt cggcgaggat cagcaacaca
    50161 accgctgaaa tgcccatacc gataatggca atgaactgcc acaatttcgc tcccgccagg
    50221 aacaacatcg ccagcgtagt cacaaacaac accaccaccg taccaaggtc tggctgtgcc
    50281 agcagtaaca ctgccaacac cagaatcacg cccatcggtt tcaggaagcc gcgcaggtta
    50341 ttacgtactt cgtcgccttt acgcaccaga tagttggcga tatagcaaaa cagcgacagt
    50401 tttgtcagct ccgcaggctg gatacgcagc aaaccgagat cgatccaacg cgatgcccct
    50461 ttaacagcgc tacccactac cagcacgatc atcagcagga tgatagatcc gagcagcatc
    50521 gtggcactgt agcgttgcca gaactccatc ggcagacgca gcgtaatgat cgccagaata
    50581 aacgccaaaa tcagatagac accatcacgc ttcgcgaaga agaacggatc gttggttaag
    50641 cgttgcccta tgggcattga cgccgaggtc accatgataa agccaatcgc cgcgaggccg
    50701 aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc tttttcccgc
    50761 gagcccatca cccagccctt tagcgccgtg gagatccaga ccaggatact gaatcctggc
    50821 aggcgcggca ttttcaggcg agggagagat aaacgcatca acctaactcc ttcgccagac
    50881 gggcaaactc attgcctcgt tgttcaaagt tcttgaactg atcaaggctg gcacaggccg
    50941 gggagagcag aaccatatcg cccggctgaa cacgcggagc cagcaagcgc atagcctgtt
    51001 ccatagtttc ggtttgttct gccacttccg ggcgtagcgc cgccagctgc gcgccgtcac
    51061 gaccgaaaca atacagacgt acgttatcgc cattcaggta acgcaccagt gggctaaagt
    51121 ccgccgattt accatcgccg cccagcaaca aatgcagcgt gccgtctacg tgcaggccat
    51181 tcagcgccgc ttccgtactg ccgacgttgg tcgcttttga atcgttaatc cagcgcacac
    51241 cgttatgctc cagcaccact tcaaagcgat gcggcagacc ggtgaaggtg gttaacgctt
    51301 tcaggctgct ggcgcgcggt aaccctgcgg catctgccag cgccagcgcc accagcgcat
    51361 tggtgtagtt atgctgcccg gaaagtttca tctctttcac attcagcact ttctcgcctt
    51421 taacccgcag ccaggtttcg ccctgctgat gattcaggtg atagtcaccc atgttgacgc
    51481 caaagctgac gcagcgttca tccgcaccgc gaatcggcat tgttaaggca tcatcagcat
    51541 taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga tactgttgta
    51601 aaccaaacgg gtagcgatcc atatgatctt cagtcacgtt cagaatggtc gctgctaccg
    51661 cctgtaagct ggaggtggtt tccagctgga agctcgacag ttccagcacg tacagttcac
    51721 actcatcatc cagtagcatc aacgcaggca ggccaatatt gccacccaca ccaacgttaa
    51781 cccccgccgc tttcgccatt tcacccacta gcgtggtgac cgtgcttttg ccgttagaac
    51841 cggtaatcgc cacaatcggt gcttgtgctt cgcgacaaaa cagctcgata tcgccaacga
    51901 tttcgattcc ggcatcagcg gcagcgctta aggatggatg cgccagtgca ataccgggac
    51961 tggcgacaat cagatctgcc gccatcagcc attcatcatt cagactgccc gtgtggcgtt
    52021 ctacggcttc gggtaattta tccaggccag gcggtgtcat acgcgtatcc ataacgcgcg
    52081 gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag cccggtgagg cccaggccga
    52141 taatgacaac atttttaccc tggtaatcag ccatgattaa cgtaccttca gcgttgccag
    52201 accaatcagg accagcatca gcgaaataat ccagaaacgc acaatgacgc gcggttccgg
    52261 ccagcctttc agttcatagt ggtgatgaat cggtgccatg cggaaaatac gttgtccgcg
    52321 cagtttaaag gagccgacct gcaggatgac agaaagcgtt tctaccacga acacgccccc
    52381 cataatcacc agcaggaatt cctgacgtag cagtacggcg ataatgccta acgcaccacc
    52441 taacgccagc gaaccgacat cgcccataaa gacctgcgcc ggataggtgt taaaccacag
    52501 gaagcccagt cctgccccga ctatcgcggt acagacaata accagttccc cggcgtgtcg
    52561 cagatacggt atgtgtaaat agctggcaaa gttcatgttg ccggtcgccc acgccaccag
    52621 cgcaaaacca ccggcgacaa atacggtcgg cataattgcc agaccgtcaa gaccatcggt
    52681 caggtttacc gcgttgccag tacccacaat gacgaagtaa gccagcagaa tgtagaacag
    52741 ccccagttgc ggcatcacat ctttaaagaa tgggaccacc agctgcgttg cgggcgtgtc
    52801 tttgccggca aggtacaaag cgaaagcaac acccagcgca atgaccgaca tccagaaata
    52861 tttccaacga gcgatcaacc ctttggtgtc tttacgcacc actttgcgat aatcatcaac
    52921 aaagccaata acaccgtaac ctaccagcac caccaacacg caccagacgt acggattgga
    52981 cgggtaagcc cacagcagca cggagatcac aatcgctgtc aggatcataa tcccgcccat
    53041 ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca ctacctgacc
    53101 aaaggaaagt ttttgcaaat gagcaatcat gcgcgggccc atccacaatg agatgaacag
    53161 cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag gaaaagacgt taaagccgga
    53221 ataatatttg accaaatgtt cggccagcca aactaacatg tcccattctc ctgtaaagcg
    53281 cgtactacct cttccatggc agcactacgt gaacccttaa ctaaaatcgt aattacctgt
    53341 tgctcagcaa tcagtgattt aagacgcgta attaacgcag ttttatcagc aaaatgttcg
    53401 ccaacgccgc tggcggtgct gatagcatgg ctttgtttac ccatgcttaa cacgcggtca
    53461 ataccagcag cttttgccgc ctcacccacc tgtacatggc aggcttcgct ttcagcgccc
    53521 agttccgcca tatcgcccac caccagcacg cggtagcccg gcatttcagc cagtacctga
    53581 acagctgcgg tcattgaacc gacattggcg ttgtaggagt cgtcgagcag caactggttt
    53641 tctgccagtt ggatggggaa cagacggcca ggaacagctt tcagatttgc cagccccgct
    53701 ttgatagcat caagcgttgc gcccacggac atggagagcg cagcggctgc cagcgcattc
    53761 gcaatattgt gacgccccgg caacggcagc agaacatcga cgctaccggt tggggtttgt
    53821 agggtaaatt ccataccgtg cgaggtcaca tggatattgg tggcggtgaa atcgctgttg
    53881 gcggcattgg gtgagaaacg ccacactttg cgtgagccaa ttacgctctg ccagttcagc
    53941 cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc gctaaagatt
    54001 tcacctttcg ctttcgcgac acccgcaagc gagccaaaac cttccagatg cgccgctgcc
    54061 aggttgttga ccagcgccgc ttccgggcga gtcagactca cagtccaggc aatctcgccc
    54121 tgatggttcg cgccaagttc aataactgcg taatcgtatt ccggcgttaa gcgcaacagc
    54181 gtcatcggta caccgatgtc gttgttgaga ttgcctgccg tataaagcgt gttgccgcac
    54241 tggcttaaaa tcgccgccgt catctcttta acggaggttt tgccggagga ccccgtcaga
    54301 gcaaccacga gcgccggaac ttgctggcga acccatgcag ccagttcacc aaacgccaga
    54361 cgcgtatcct tgacgattaa ctgcggcagg tcgatgtcca gcggacggct aaccagtagt
    54421 gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcgt gggcatcaaa acgttcacct
    54481 ttcagggcaa caaacaggca gcccggcgtc agttttcggg tatcagtggt tacagcatca
    54541 agggtgatat ctgcaccttg cagttcaccg ttgagaatat cggtaagttg gctaagggtt
    54601 acgctaatca tgcaatcccc cccagcagac gcgccaccgt gacgcgatcg gagtagtcca
    54661 gacgctgatt gccaacaatc tggtaatcct catggccttt gcccgcgacc agtaccacat
    54721 cattctcttt agcctgcata acggcgcaag tcaccgcttc agcacggcct tccatcactt
    54781 tggcatgtcc ggcatctaac attcccgcca gaatatcgtt gatgatggca cgcggttctt
    54841 cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg gcaattgcgc
    54901 ccatcagtgg acgcttacct ttatcgcgat cgccaccaca gccaaagacg caccacagct
    54961 tgcccgcaca gtgcaggcgc gccgcctgta aggctttttc cagtgcatcc ggcgtatgcg
    55021 cgtaatccac caccaccgtc ggtttgcctg gcgcagtgaa cacttccata cgtccgcaaa
    55081 ccggttgcag acgcgcggcg gttttcagca gatcagccag tggatagccc agcgccagca
    55141 gtgttgccag cgcgagcaac aggttgctga cgttaaaagc gcccatcaga tggctttcaa
    55201 tttcgccatc gccccagctt gaactaaagc gaatggtcgc gccgctgtcg tgatagttca
    55261 cttcggtcgc tttcaaccag cgtccgtgac agttcggatt gatatgatct tccattgata
    55321 ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc cacttcatcg tcggcgttaa
    55381 taatcgcctg accgcaatga tgttcagaat aaagcagcca tttcgcagct tcgtagtgtt
    55441 ccatatcacc atgataatca aggtgatcgc ggcttaagtt ggtaaagacc gacgccggaa
    55501 atttcaatgc cgccacacgg tgctgtacca gcccgtggga ggaaacttcc attgcgcaaa
    55561 aagtcgcgcc ctgatccacc agccccgcca gctcatgctg aacatcaact gccgaaccgg
    55621 tagtattttc tgtcgggatc actttcccca gcagaccgtt accaacggtg cccattaccg
    55681 cgctggtttc gccaagcagt tggctccact gcgccagaag ctgggtcgtc gtggttttgc
    55741 cgttggtgcc cgttacgcct acgagacgta aattgtcaga gggttcatgg taaaagcggc
    55801 ccgccagtgc agataaacgc tcgttgagct ggctgagata gatgaccggt acgccgtgca
    55861 tttcacggat ttcaccgtcg gtcgcctcat ctttcgcctc tgcaataatg gcagccacac
    55921 cttgcgctat cgcctgcggg atatatcgac gcccgtccgc ctgatgacct actacagcta
    55981 caaagagatc gcccgccgca gccacacggc tgtcgagtgt catctctcgc agtgctcgcg
    56041 aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc
    56101 ccctcgcctt gattaatcac aaattcattt ttatcgcccg ttgtcagcgc atccggctcg
    56161 atgttcatgg tacgcaatac gccgcccatg atggcaccaa agaccggcgc ggaaacggcg
    56221 ccgccgtagt atttacccgc ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc
    56281 tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt tattgatgta gcgaccgtcc
    56341 ggcccgacct ttttcgcggt accggtttta atggcgatac gatagccttt aatcgccgcc
    56401 ttcacgccgc cgccgcctgg tagcgccacg ctttccatca tatgcaccac ggtgcgaaca
    56461 atggattccg ggaagacacg ttcaccggga accggggggt caactttggt aatcgacagt
    56521 gggcgataaa tgccgtagct gccgatagtt gcgtagactc gcgctaactg taacggtgtt
    56581 accattagcc cgtagccgaa agagaaggtg gccctctcta tgtcagacca ccgttgtttt
    56641 tgaggatata agccactgcg ttctccgacc aaccccaaat tggtcgcttt tcccagtcca
    56701 aaacgtgagt aagtatctac taacactgag gacggcatcg ttaacgccag cttggaaaca
    56761 ccgacgttac tcgacttctg taataccccg gtcagggtta attcgctgta gcgtgccacg
    56821 tctttgattt cgtggccgtt aattcgataa ggaatggtat tgagtaccga gttttcccgc
    56881 accacgccac gttgcaacgc ggtcatcacc accatcggtt taaccgttga gcccggttca
    56941 aacacgtcgg tgatggtacg gttacgcatc gcctctttcg gcgtgccgct cagattgtta
    57001 gggttgtatg acgggctgtt agccatcgcc agcacttcac ctgtgttgac atccaccagc
    57061 acggcgctac cagattcagc cttgttaaag gccaccgcgt tgttcagttc gcgataaacc
    57121 agcgcctgca ggcgttcatc aatactcagc gccaggttgt gcgctgcctg gctgtcagta
    57181 gaagaaatat cttcaattac gcgaccatag cggtctttac gcacaatgcg ctcacccggc
    57241 tgcccggtaa gccatttatc gaaactcttc tcaacgccct caatcccttg actatcgacg
    57301 ttagtaaagc cgatgaggtg agcagtcact tcgccggacg gatagtaacg gcgagactct
    57361 tcacgcagat gaatccccgg cagtttcagt tttttgatgt agtccgccat gtcagagttt
    57421 acctgacgcg ccagataaat aaagcgccct ttcgggttgg cgttaatgcg ggcagaaagc
    57481 tgatccagcg gaatattgag cgcgttagcc agcgccttcc agcggtcacc gacgctgata
    57541 ccgccagcgt catgcacttc tttcgggtca gcccaaatcg cttttaccgg cacgctcacc
    57601 gctaacgggc gaccagaacg gtcagtaatc atgccgcggg aggtggaaac ttgctgaacg
    57661 cgaagagaac gcatgtcgcc ctctttcacc agcatatccg gggagataac ttgtaaccac
    57721 gctacgcgtc cgagcagaaa agccagcgcc aggagaatac agccgcataa caacgcaaaa
    57781 cgccaactga taaagttggc atgttcttcc tgacgttttg gtttctgcgt tttcgccgct
    57841 gctttcatgc gtcgcgttta tccttatttt tgcactacga tattttcttg tgacggatca
    57901 acatgctgca tttgcagctt ttccgtggcg atcctttcca cccggctatg gtcgccgagc
    57961 gcattctctt caaggatcag gttgcgccat tcaatgtcta aagcatctcg ctccagcacc
    58021 agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac cacggtcacc
    58081 gccgtcaaaa taatgcaaat gaacaggcag agtggcagct tcccaaatcg caaaagatcg
    58141 tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg
    58201 cttagagctt ctgtcactct gctgatcatg cgttcgtcct ctctgcaata cgcagaactg
    58261 aactacgggc acgagggttc tcagccacct cttcttcgcc cggcattaac ttgcctagtg
    58321 ctcgcagctg acggccaccc agttttttga gctgctcttc agtcatcggt aaccctgccg
    58381 gaacttgcgg accgcggctg ttttcacgca taaaacgttt cacaatacgg tcttccagcg
    58441 agtggaagct gatgatcgaa agccgcccac ccggggccag cacgttgagc gagcttttta
    58501 gcgcctgctc tatctcctcc agttcactgt ttacccaaat gcgcaccgcc tggaaggtac
    58561 gggtcgcggg atgtttaaac ttatctttca ccggcgttgc agcagccacg acttccgcca
    58621 gttctttggt gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc
    58681 gtttggcaaa atgctcttca ccataggttt tcaataccca ggcgatatcg gcttcttctg
    58741 cggtttgtag ccattcagcg gctgactgcc cacgggttgg gtccatacgc atgtccagcg
    58801 gaccatcgcg cataaaggaa aagccgcgtt cagcatcatc aagttgcggt gaagagacgc
    58861 caagatcgag gagaatgccg tcgatcttgc caataagatc gcgctcggca acgtattcgc
    58921 ccagcgcgga gaaaggtccg tggatgatgg agaagcgcgg atcatcaata gtcttcgcaa
    58981 cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg
    59041 agaggatcag acgtgagtga ccaccgcgac caaaagtccc atcaatgtag atgccatcag
    59101 gacggatatt gaggccatta acggcttcat ccagcagcac cgtagtatgt ttatagtttt
    59161 ccatcatttt atagagacaa gtcctgcagt cgctccgata agtctccggt agccaactgc
    59221 tctgcgtcga tatcttcctt gacctgttga tgccaggttg tttcatccca cagctcaaac
    59281 ttgttgaact gtccaaccag catcacttct tttgtcagcc cggcatgttg ccgcagtact
    59341 ggcgcgatta acaatcgacc tgcgccatcc atctgacatt cgctggcatg acctaacagt
    59401 aggcgctgca cacggcgctc aaccgggttc atgctcaaca gacgcgataa tttttgctcg
    59461 ataatttccc attcaggcag ggggtaaagc agcaggcacg ggtgatgaat gtcaatggtg
    59521 caaaccattt gaccggcagc gttctcaagc agctgttccc gataacgggt aggcactgat
    59581 aagcgccctt tgctgtcgag attgactaac gttgctcccc ggaacatgcc agcctcaccc
    59641 cttattccca ctttgcccca caatttccca ctgaaaggag tttacggagc ggaggaaaag
    59701 cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc cgatgtttac ggctaatatc
    59761 agcgttgttt attttacgtc caacgtgcga agcaaattaa cgttatgaat agctgtaaga
    59821 aaaaatccct catcgtcatc actgttttaa aatgactcac tattttccac agcaaatata
    59881 aagtgtcagt ttgcgacgcg agcggcattt taggacatat cttccccggt taacagtcct
    59941 tgttacgtct gtgtggcgct tgatgccagc gggtttgcgc aaaagcaaag aaaagtgttt
    60001 gttaaatcgg caattcatcg ccgcatgtaa caaaataatg caaaaacgcg ctgtcgatat
    60061 cacttaatta ttgtgaattt cagctggtga gttaattaac gggcgggttt aaaaccattt
    60121 atcaataata gtcaggaata gtcttattta ctttaaggat attgat
//
LOCUS       DAJTRN010000012        59740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRN010000012 DAJTRN010000000
VERSION     DAJTRN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            411..1700
                     /gene="sdaC"
                     /locus_tag="OO328_000775"
     CDS             411..1700
                     /gene="sdaC"
                     /locus_tag="OO328_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5929446.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1758..3125
                     /gene="sdaB"
                     /locus_tag="OO328_000776"
     CDS             1758..3125
                     /gene="sdaB"
                     /locus_tag="OO328_000776"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5929447.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3236..3991
                     /gene="xni"
                     /locus_tag="OO328_000777"
     CDS             3236..3991
                     /gene="xni"
                     /locus_tag="OO328_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5929448.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4044..5192)
                     /gene="fucO"
                     /locus_tag="OO328_000778"
     CDS             complement(4044..5192)
                     /gene="fucO"
                     /locus_tag="OO328_000778"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5929449.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5220..5867)
                     /gene="fucA"
                     /locus_tag="OO328_000779"
     CDS             complement(5220..5867)
                     /gene="fucA"
                     /locus_tag="OO328_000779"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5929450.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6414..7730
                     /gene="fucP"
                     /locus_tag="OO328_000780"
     CDS             6414..7730
                     /gene="fucP"
                     /locus_tag="OO328_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5929451.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7763..9538
                     /gene="fucI"
                     /locus_tag="OO328_000781"
     CDS             7763..9538
                     /gene="fucI"
                     /locus_tag="OO328_000781"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5929452.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9647..11066
                     /gene="fucK"
                     /locus_tag="OO328_000782"
                     /pseudo
     CDS             9647..11066
                     /gene="fucK"
                     /locus_tag="OO328_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11068..11490
                     /gene="fucU"
                     /locus_tag="OO328_000783"
     CDS             11068..11490
                     /gene="fucU"
                     /locus_tag="OO328_000783"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5929453.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11548..12279
                     /gene="fucR"
                     /locus_tag="OO328_000784"
     CDS             11548..12279
                     /gene="fucR"
                     /locus_tag="OO328_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5929454.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12323..13423)
                     /gene="rlmM"
                     /locus_tag="OO328_000785"
     CDS             complement(12323..13423)
                     /gene="rlmM"
                     /locus_tag="OO328_000785"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5929455.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13416..13811)
                     /locus_tag="OO328_000786"
     CDS             complement(13416..13811)
                     /locus_tag="OO328_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5929456.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13830..14747)
                     /gene="gcvA"
                     /locus_tag="OO328_000787"
     CDS             complement(13830..14747)
                     /gene="gcvA"
                     /locus_tag="OO328_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5929457.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15098..15325)
                     /locus_tag="OO328_000788"
     CDS             complement(15098..15325)
                     /locus_tag="OO328_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5929458.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15517..16722
                     /gene="csdA"
                     /locus_tag="OO328_000789"
     CDS             15517..16722
                     /gene="csdA"
                     /locus_tag="OO328_000789"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5929459.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16722..17165
                     /gene="csdE"
                     /locus_tag="OO328_000790"
     CDS             16722..17165
                     /gene="csdE"
                     /locus_tag="OO328_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5929460.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17216..18022)
                     /gene="tcdA"
                     /locus_tag="OO328_000791"
     CDS             complement(17216..18022)
                     /gene="tcdA"
                     /locus_tag="OO328_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5929461.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18260..19357)
                     /gene="mltA"
                     /locus_tag="OO328_000792"
     CDS             complement(18260..19357)
                     /gene="mltA"
                     /locus_tag="OO328_000792"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5929462.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19566..19642
                     /locus_tag="OO328_000793"
     tRNA            19566..19642
                     /locus_tag="OO328_000793"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19600..19602,aa:Met,seq:cat)
     gene            19676..19752
                     /locus_tag="OO328_000794"
     tRNA            19676..19752
                     /locus_tag="OO328_000794"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19710..19712,aa:Met,seq:cat)
     gene            complement(19827..21080)
                     /gene="amiC"
                     /locus_tag="OO328_000795"
     CDS             complement(19827..21080)
                     /gene="amiC"
                     /locus_tag="OO328_000795"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5929463.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21312..22643
                     /gene="argA"
                     /locus_tag="OO328_000796"
     CDS             21312..22643
                     /gene="argA"
                     /locus_tag="OO328_000796"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5929464.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22705..24531)
                     /gene="recD"
                     /locus_tag="OO328_000797"
     CDS             complement(22705..24531)
                     /gene="recD"
                     /locus_tag="OO328_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5929465.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24531..28073)
                     /gene="recB"
                     /locus_tag="OO328_000798"
     CDS             complement(24531..28073)
                     /gene="recB"
                     /locus_tag="OO328_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5929466.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28066..30954)
                     /gene="ptrA"
                     /locus_tag="OO328_000799"
     CDS             complement(28066..30954)
                     /gene="ptrA"
                     /locus_tag="OO328_000799"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5929467.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31130..34498)
                     /gene="recC"
                     /locus_tag="OO328_000800"
     CDS             complement(31130..34498)
                     /gene="recC"
                     /locus_tag="OO328_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5929468.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34511..34861)
                     /locus_tag="OO328_000801"
     CDS             complement(34511..34861)
                     /locus_tag="OO328_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5929469.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34846..35253)
                     /locus_tag="OO328_000802"
     CDS             complement(34846..35253)
                     /locus_tag="OO328_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5929470.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35250..35813)
                     /locus_tag="OO328_000803"
     CDS             complement(35250..35813)
                     /locus_tag="OO328_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5929471.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35804..36274)
                     /locus_tag="OO328_000804"
                     /pseudo
     CDS             complement(35804..36274)
                     /locus_tag="OO328_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36458..37252)
                     /gene="thyA"
                     /locus_tag="OO328_000805"
     CDS             complement(36458..37252)
                     /gene="thyA"
                     /locus_tag="OO328_000805"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5929472.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37259..38134)
                     /gene="lgt"
                     /locus_tag="OO328_000806"
     CDS             complement(37259..38134)
                     /gene="lgt"
                     /locus_tag="OO328_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5929473.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38285..40531)
                     /gene="ptsP"
                     /locus_tag="OO328_000807"
     CDS             complement(38285..40531)
                     /gene="ptsP"
                     /locus_tag="OO328_000807"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5929474.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40544..41074)
                     /gene="rppH"
                     /locus_tag="OO328_000808"
     CDS             complement(40544..41074)
                     /gene="rppH"
                     /locus_tag="OO328_000808"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5929475.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41759..42448
                     /gene="mutH"
                     /locus_tag="OO328_000809"
     CDS             41759..42448
                     /gene="mutH"
                     /locus_tag="OO328_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5929476.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42517..43230
                     /locus_tag="OO328_000810"
     CDS             42517..43230
                     /locus_tag="OO328_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5929477.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43368..43586
                     /gene="ygdR"
                     /locus_tag="OO328_000811"
     CDS             43368..43586
                     /gene="ygdR"
                     /locus_tag="OO328_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5929478.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43694..44734
                     /locus_tag="OO328_000812"
     CDS             43694..44734
                     /locus_tag="OO328_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5929479.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44767..45957)
                     /gene="lplT"
                     /locus_tag="OO328_000813"
     CDS             complement(44767..45957)
                     /gene="lplT"
                     /locus_tag="OO328_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5929480.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45950..48109)
                     /gene="aas"
                     /locus_tag="OO328_000814"
     CDS             complement(45950..48109)
                     /gene="aas"
                     /locus_tag="OO328_000814"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5929481.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48695..49726
                     /gene="galR"
                     /locus_tag="OO328_000815"
     CDS             48695..49726
                     /gene="galR"
                     /locus_tag="OO328_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5929482.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49733..50995)
                     /gene="lysA"
                     /locus_tag="OO328_000816"
     CDS             complement(49733..50995)
                     /gene="lysA"
                     /locus_tag="OO328_000816"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5929483.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51117..52052
                     /gene="lysR"
                     /locus_tag="OO328_000817"
     CDS             51117..52052
                     /gene="lysR"
                     /locus_tag="OO328_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5929484.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52039..52731)
                     /gene="ygeA"
                     /locus_tag="OO328_000818"
     CDS             complement(52039..52731)
                     /gene="ygeA"
                     /locus_tag="OO328_000818"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5929485.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52860..54278)
                     /gene="araE"
                     /locus_tag="OO328_000819"
     CDS             complement(52860..54278)
                     /gene="araE"
                     /locus_tag="OO328_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5929486.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54593..55354)
                     /gene="kduD"
                     /locus_tag="OO328_000820"
     CDS             complement(54593..55354)
                     /gene="kduD"
                     /locus_tag="OO328_000820"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5929487.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55384..56220)
                     /gene="kduI"
                     /locus_tag="OO328_000821"
     CDS             complement(55384..56220)
                     /gene="kduI"
                     /locus_tag="OO328_000821"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5929488.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56507..57688)
                     /locus_tag="OO328_000822"
     CDS             complement(56507..57688)
                     /locus_tag="OO328_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5929489.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57943..59172
                     /locus_tag="OO328_000823"
                     /pseudo
     CDS             57943..59172
                     /locus_tag="OO328_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14750 a        16035 c        14562 g        14393 t
ORIGIN      
        1 gcaacacaaa cgtttcccta ttttcataaa ccgtatttta tcgctatgaa aaagaaatta
       61 tcgccatgat taactaaaag tattgatttt ttcagttcaa cctacatata ttgcgcgccc
      121 cggaagaagt cagatgtcgt ttaatgggca aatattgccc ttaaattctc ttttactttt
      181 gatttacaga gtaaagcgtt gggataatct atcttccaag cagattattg tatttgggat
      241 caagatcact gatagataca taacttgtgt gtatctttcc gccctcaaat tattacggcg
      301 gtaaatgatt aagccatcgc cgatagacag atttcatttt tacggtcagg caccttcccg
      361 ggctgaactg gctaaaagct gaattatttg cattcctcca ggagaaatag atggaaacga
      421 ctcaaaccag cacgattgcg tcgaaagact ctcgtagtgc ctggcgcaag acagacacca
      481 tgtggatgct gggcctttac ggcacggcaa tcggcgcggg cgtgctgttc ctgccaatca
      541 acgccggtgt tggcggtatg atcccgctga tcatcatggc tatccttgcg ttcccgatga
      601 cgttttttgc tcaccgcggc ctgactcgct tcgtactgtc tggtaaaaac ccgggcgaag
      661 acatcaccga ggttgtagaa gaacactttg gtattggcgc aggtaaactg attaccctgc
      721 tctacttctt cgctatctac ccgatcctgc tggtttatag cgtggcaatc accaataccg
      781 ttgaaagctt catgtctcac cagctgggta tgacgccacc gccgcgtgcg attctgtcgc
      841 tgatcctgat cgtgggtatg atgaccatcg ttcgctttgg tgagcagatg atcgttaaag
      901 cgatgagtat tctggtattc ccgtttgttg gcgtactgat gctgctggct ctgtacctga
      961 tcccgcagtg gaacggcgca gcactggaaa cgctgtctct ggacactgca tctgcaaccg
     1021 gaaacggtct gtggatgacc ctgtggctgg caattccggt aatggtgttc tcgttcaacc
     1081 actctccgat catctcttct ttcgccgttg cgaagcgtga agagtacggc gatatggcag
     1141 aacagaaatg ctcgaagatc ctggcattcg cacacatcat gatggtgctg accgtaatgt
     1201 tcttcgtctt cagctgcgta ctgagcctga ctccggcaga cctggctgcg gctaaagagc
     1261 agaacatctc gattctgtct tacctggcta accactttaa cgcaccggtt atcgcgtgga
     1321 tggctccgat tatcgcgatt atcgctatca ccaaatcctt cctcggccac tacctgggcg
     1381 cacgtgaagg cttcaacggt atggtgatta aatctctgcg tggtaaaggt aagtctatcg
     1441 aaatcaacaa gctgaaccgt atcactgcgc tgttcatgct ggtaacgacc tggattgttg
     1501 ccaccctgaa cccgagcatc ctgggtatga ttgaaaccct gggcggccca atcatcgcga
     1561 tgatcctgtt cctgatgccg atgtacgcaa ttcagaaagt accggcaatg cgtaagtaca
     1621 gcggtcacat cagcaacgta ttcgttgtcg tgatgggtct gattgcaatc tccgcaatct
     1681 tctactctct gttcagctaa gtcctttcgc gccgctttcg ggcggcgctt cctccgtttt
     1741 aacgcgatgt atttcctatg attagcgtat tcgatatttt caaaatcggc attggccctt
     1801 ccagttctca taccgttgga ccaatgaaag cgggtaaaca atttaccgac gatctgattg
     1861 cccgtaacct gcttaaagac gtgacccgcg tggtggttga cgtgtacggc tcgctctctc
     1921 tgactggtaa aggccaccac acagatatcg ccattattat gggcctggcg ggtaacctgc
     1981 cggataccgt ggatatcgat tccatccccg gttttattca ggatgtgaat actcatggtc
     2041 gcctgatgct ggcaaacggt cagcatgaag tggagttccc ggttgatcag tgcatgaact
     2101 tccatgcaga caacctttct ctgcatgaaa acggtatgcg cattaccgcg ctggcgggcg
     2161 ataaagtcgt ttacagccag acttactact ctattggcgg tggctttatc gttgatgaag
     2221 agcattttgg ccagcaggat agcgcaccgg ttgaagttcc ttatccgtac agttcagcag
     2281 ccgatctgca aaaacattgt caggaaaccg ggctgtcact ctctggcctg atgatgaaaa
     2341 acgagctggc gctgcacagt aaagaagagc tggaacagca cctggcgaac gtctgggaag
     2401 tcatgcgtgg cggtattgag cgcggtattt ccaccgaagg cgtgttgcct ggcaaactgc
     2461 gcgttccacg ccgtgctgcg gcactacgcc ggatgctggt cagccaggat aaaaccacca
     2521 ctgacccgat ggcggttgtt gactggatca acatgtttgc actggcagtg aacgaagaga
     2581 acgctgctgg cggtcgcgtg gtgactgcgc cgactaacgg tgcgtgcggg attatcccgg
     2641 ctgtgctggc gtactacgat aagtttatcc gcgaagtgaa cgctaactca ctggctcgtt
     2701 acctgctggt agccagcgcc attggttctc tttataagat gaacgcgtcg atttctggtg
     2761 ccgaagtggg ttgccagggt gaagttggcg tggcgtgctc tatggcggca gctggtctgg
     2821 cagaactgtt aggcgcaagc ccggcgcagg tttgcatcgc agcggaaatc gcgatggagc
     2881 acaacctcgg tctgacttgt gacccggtcg cggggcaggt tcaggtaccg tgcattgagc
     2941 gtaacgccat tgcagcagtg aaagcggtga acgccgcgcg tatggcactg cgccgtacca
     3001 gcgaaccgcg cgtctgcctc gataaagtta tcgaaaccat gtacgaaaca ggtaaagaca
     3061 tgaacgccaa gtaccgcgaa acctctcgcg gcggcctggc gatgaagatc gttgcctgcg
     3121 attaatctct cccagaggcc tcgttttgcg aagcctcttc ctgttttctc aaccagccat
     3181 agcctgttcc ggcatcgaat gttaccctta tcgcctgatc tttaaggggg ttatcgtggc
     3241 tgttcatttg cttattgtcg atgcactgaa tcttatccgt cgcattcatg ccgttcaggg
     3301 ttcgccctgt gtcgaaacct gccagcatgc gctcgatcag ctcattatgc acagccagcc
     3361 aacccacgcg gtcgccgttt ttgatgatga aaaccgcagt agcggctggc gtcatcagcg
     3421 tttaccagat tacaaagcgg atcgaccgcc aatgccggaa gagttgcacg acgagatgcc
     3481 tgcattacgc gccgcctttg agcaacgcgg cgtcccgtgc tggtcagcca gcggcaacga
     3541 agccgatgac ttagccgcca cactggcggt caaagtgaca caggccgggc atcaggcaac
     3601 gattgtttcg acagataaag gctactgtca gttactttca ccgacattac gtattcgtga
     3661 ttacttccag aaacgttggc tggatgcgcc atttatcgat aaagaatttg gcgttcaacc
     3721 gcagcagttg cccgattact ggggacttgc ggggatcagc agttcaaagg taccgggtgt
     3781 tgcgggaatc ggaccaaaaa gcgccacgca gctgctggtc gagtttcaga gtctggaagg
     3841 gatatatgag aatctggatg cggttgccga aaagtggcgc aaaaaattag aaacccataa
     3901 agagatggcg tttctgtgcc gcgatattgc ccgcttacaa accgatttgc atatcgacgg
     3961 caatttacag caattgcggt tggtacggta acggcgagcc ggatacgccg caaatcgtat
     4021 ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac aatatcctca
     4081 agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc ctgagccagt
     4141 gccgaaatgt cttccttgcg tacaccaaca tcacgcaaat gtggcggaat accgacatca
     4201 cggttgaggg caaacaccgc ttcaacagcg gcattacgcg ccccttccag gctcatacct
     4261 tccactttca cgcccataac gcgcgcgata tcgcggtact tctcaccggt aaagtcagcg
     4321 ttatagcgca tgacatgcgg taacaggatg gcgttcgcaa caccgtgtgg agtgttataa
     4381 aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt cgagaagccc
     4441 atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc accagcaacc
     4501 gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagtgc atcggttagt
     4561 gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc aacacccgtc
     4621 gcggctttca gcgccggagg cataccatcc atcatgtcag catcaataaa cgccacctgc
     4681 gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tttcttcgtc agtgatcacg
     4741 tagttaatgg tcacttctgc cgcagtacct gctgtggtgg ggattgccag aatcggtaca
     4801 ctgggtttat tggtcgggga aagcccttcc aggctacgca catcggcaaa ctccgggttg
     4861 ttgctgataa tgccaattgc tttacaagta tcctgtggag aaccaccacc aatagcgatc
     4921 aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac agtaattgtt
     4981 gggttgggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc catcttatcg
     5041 gtcactttcg ccaccacgcc gcattgcacc agcgttttat cggtgacgat cagcgccttc
     5101 tgataaccac ggcgtttcac ctcatcggtt aaagccccaa cagcaccccg accaaaccat
     5161 gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc tttacgaaat
     5221 tactcttcaa tccgtaaccc ataggttttg aatttctcca gcactacggc aatctcttca
     5281 tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag ttgcgccagc
     5341 acttcaactt catgcgccag ccataacgct ttttccagat tcacctcaca agcgataagc
     5401 ccatgatgtt gtaacaaagt tgccttacga tttttgagag ccagcgcaac atgttcagaa
     5461 agttcgcgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc gccagccgcc
     5521 gcaatcatgt agtgaatagc ggggatcgat cggttaagaa tggaaactgc cgtgcaatga
     5581 acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc tgccatatgg
     5641 aaacgccatt cgcttgaggg gagctttcct tcctcatgtt taccgttgcc atcaataaag
     5701 acaatatgcg actccgtcag tttttcatat ggaatgcctg taggcgtaat cagcatccca
     5761 tcctgataac gtacactgac gttccccgct gtcccctggt tcagtcccag gcgggtcatt
     5821 tccaggcaag tgtcaataat ctgacgagca agtttatttc gttccattag ctacctctct
     5881 ctgattcaaa acagggcaat aatgttgttc ctttcacact attgaattag ccgtttaatt
     5941 acccaccatc ttcttcctga ttaacaagaa agaaattcac aagcttatat tttgtgacct
     6001 agctcaacta atcacagtaa ataactgcaa gttctctttt tataaccccg ttaaaaatga
     6061 ccgctcttaa aaatatttat caaaacggtc atttttccat ttcccctcgc cagaaatgcc
     6121 cgttttcggc acaaacaatt aatacggtca tctggtttgt gttttttatg atttattttc
     6181 tgaaacgggc atgaaatttc gattattaaa gtgatggtag tcacataaag tcaccttcca
     6241 gctaataagt gtgaccgccg tcatattaca gagcgttttt tatttgaaaa tgaatccatg
     6301 agttcatttc agacaggcaa atattcaccg atatgaagcc cgaactcgct ggttttgcac
     6361 ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac ctaagaggat gctatgggaa
     6421 acacatcaat acaaacgcag agttaccgtg cggtagataa agatgcaggg caaagcagaa
     6481 gttacattat tccattcgcg ctgctgtgct cactgttttt tctttgggcg gtagccaata
     6541 accttaacga cattttatta cctcaattcc agcaggcttt tacgctgaca aatttccagg
     6601 ctggcctgat ccaatcggcc ttttactttg gttatttcat tatcccaatc cctgctggga
     6661 tattgatgaa aaaactcagt tataaagcag ggattattac cggattattt ttatatgcct
     6721 tgggcgctgc attattctgg cccgctgcag aaataatgaa ctacacctta tttttagttg
     6781 gcctatttat tattgcagcc ggattaggtt gtctggaaac tgccgcaaac ccttttgtta
     6841 cggtattagg gccggaaagc agtagtcact tccgcttaaa tcttgcgcaa acatttaact
     6901 cgtttggcgc aattatcgcg gttgtctttg ggcaaagtct tattttgtct aacgtgccac
     6961 atcaatcgca agacgttctc gataaaatgt ctccagagca attgagtgcg tataaacaca
     7021 gcctggtatt atcggtacag acaccttata tgatcatcgt ggctatcgtg ttactggtcg
     7081 ccctgctgat catgctgacg aaattcccgg cattgcagag tgataatcac agtgacgcca
     7141 aacaaggatc gttctccgca tcgctttctc gtctggcgcg tattcgtcac tggcgctggg
     7201 cggtattagc gcaattctgc tatgtcggcg cacaaacggc ctgctggagc tatttgattc
     7261 gctacgctgt agaagaaatt ctaggtatga ctgcaggctt tgccgctaac tatttaaccg
     7321 gaaccatggt gtgcttcttt attggtcgtt tcaccggtac ctggctcatc agtcgcttcg
     7381 caccacacaa agtcctggcc gcctacgcat taatcgctat ggcactgtgc ctgatctcag
     7441 ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt atgcagcgcc tttatgtcga
     7501 ttcagtaccc aacaatcttc tcgctgggca ttaagaatct cggccaggac accaaatacg
     7561 gttcgtcctt catcgttatg accattattg gcggcggtat tgtcactccg gtcatgggtt
     7621 ttgtcagtga cgcggcgggc aacatcccca ctgctgaact gatccccgca ctctgcttcg
     7681 cggtcatctt tatctttgcc cgtttccgtt ctcaaacggc aactaactga acatattttc
     7741 cgaataaagt gaggaatctg taatgaaaaa aatcagctta ccgaaaattg gtatccgccc
     7801 ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt gaagaacaaa caatgaatat
     7861 ggcgaaagct acggccgcac tgctgaccga gaaactgcgc catgcctgcg gagctgccgt
     7921 cgagtgtgtc atttccgata cctgtatcgc gggtatggct gaagccgctg cttgcgaaga
     7981 aaaattcagc agccagaatg taggcctcac cattacggta acgccttgct ggtgctatgg
     8041 cagtgaaacc atcgacatgg atccaacccg cccgaaagcc atttggggct ttaacggcac
     8101 tgaacgcccc ggcgctgttt acctggctgc ggctctggca gctcacagcc agaaaggcat
     8161 cccggcattc tccatttacg gtcatgacgt tcaggatgcc gatgacacat cgattcctgc
     8221 cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt ttggccgtcg ccagcatgaa
     8281 aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt atcgccggtt ctattgttga
     8341 tcacaacttc tttgaatcct ggctgggaat gaaagtccag gcggtggata tgaccgaact
     8401 gcgtcgccgt atcgatcaga agatttacga cgaagccgaa ctggaaatgg cactggcctg
     8461 ggctgataaa aacttccgct atggcgaaga tgaaaataac aaacagtatc aacgcaatgc
     8521 cgagcaaagc cgcgcagttc tgcgcgaaag tttactgatg gcgatgtgta tccgcgacat
     8581 gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg gaagaatcac ttggctacaa
     8641 cgccatcgct gcgggcttcc aggggcaacg tcactggacc gatcaatatc ccaatggtga
     8701 caccgccgaa gcgatcctca acagttcatt tgactggaat ggcgtgcgcg aaccctttgt
     8761 cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg ctaatgggtc accagctcac
     8821 cggcaccgct caggtatttg ccgatgtgcg tacctactgg tcaccagaag caattgagcg
     8881 tgtaacgggg cataaactgg acggactggc agaacacggc atcatccatt tgatcaactc
     8941 aggttctgct gcgctggacg gttcctgtaa acaacgcgac agcgaaggta acccgacgat
     9001 gaagccgcac tgggaaatct ctcagcaaga ggctgacgct tgcctcgccg ctaccgaatg
     9061 gtgcccggcg atccacgaat acttccgtgg cggcggttac tcttcccgct tccttaccga
     9121 aggcggcgtc ccgttcacca tgactcgtgt caacatcatc aaaggcctgg gaccggtact
     9181 gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat gtgcatgaca tcctcaacaa
     9241 acgcaccaac tcaacctggc caaccacctg gtttgcaccg cgcctcactg gtaaagggcc
     9301 gtttacggat gtgtattcgg taatggcgaa ctggggtgcc aaccacggag ttctgaccat
     9361 cggccacgtt ggcgcagact ttatcaccct tgcctccatg ctgcgtatcc cggtatgtat
     9421 gcacaacgtt gaagagacca aagtgtatcg tccttctgcc tgggctgcgc acggcatgga
     9481 tattgaaggc caggattacc gcgcttgcca gaactacggt ccgttgtaca agcgttaata
     9541 cattacaccc tgatgtgatg cccggtcgct ccggctaccg ggcctgaaca agcaagagtg
     9601 gttagccgga taaacaatgt tatccggcta tattgcagga gcgattatga aacaagaagt
     9661 tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc atcgcggtta atcggcaggg
     9721 aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat atcgcgatgg aaaacaacac
     9781 ctggcaccag tggtctttag actgccattt tgcaacgctt tgctgattgc tgtcggcaaa
     9841 tcaatagtga actgactgaa tgccacatcc gcggtatcgc cgtcaccacc tttagtgtgg
     9901 atggcgctct ggtagataag caaggcaatc tgctctatcc gatcattagc tggaaatgtc
     9961 cgcgaacagc agcggttatg gacaatattg aacggttaat ctccgcacag cggttgcagg
    10021 ctatttctgg cgtcggagcc tttagtttca atacgttata taagttggtg tggttgaaag
    10081 aaaatcatcc acaactgctg gaacgcgcgc acgcctggct cttcatttcg tcgctgatta
    10141 accaccgttt aaccagcgaa ttcactactg atatcacgat ggccggaacc agccagatgc
    10201 tggatatcca gcaacgcgat ttcagtccgc aaattttaca agccaccggt attccacgcc
    10261 gactcttccc tcgtctggtg gaagcgggtg aacagattgg tacgctacag aacaacgccg
    10321 cagcaatgct cggcttaccc gttggcatac cggtgatttc cgcaggtcac gatacccagt
    10381 tcgccctttt tggcgctggt gccgaacaaa atgaacccgt gctctcctcc ggtacatggg
    10441 aaattttaat ggttcgcagc gcctaggttg atacttcgct gttaagtcag tacgccggtt
    10501 ccacctgcga actagatagc caggcatggt tgtataaccc aggtatgcaa tggctggcat
    10561 ccggcgtgct ggaatgggtg agaaaactgt tctggacggc tgaaacaccc tggcaaatgt
    10621 tgattgaaga agctcgtctg atcgcacctg gcgcggatgg tgtgaaaatg cagtgtgatt
    10681 tattgtcgtg tcagaacgct ggctggcaag gagtgacgct taataccacg cgggggcatt
    10741 tctatcgcgc ggcgctggaa gggttaactg cgcaattaca gcgcaatcta cagatgctgg
    10801 aaaaaatcgg gcactttaaa gcctatgaat tattgttagt cggtggagga agtcgcaaca
    10861 cattgtggaa tcagattaaa gccaatatgc ttgatattcc ggtaaaagtt ctcgacgatg
    10921 ccgaaacgac cgtcgcagga gctgcgctgt tcggttggta tggcgtaggg gaatttaaca
    10981 gcccggaaga agcccgtgca cagattcatt atcagtaccg ttatttctac ccgcaaactg
    11041 aacctgaatt tatagaggaa gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc
    11101 gaactattga aagtgctggc agagatggga catggagatg aaattatttt ttccgatgct
    11161 cactttcccg cccattcgat ggaaccgcag gtgatccgcg ctgatggcct gttggtgagc
    11221 gacttgctcc aggcgattat cccgttattt gagctggaca gttatgcccc gccgctggtg
    11281 atgatggcgg cggtagaggg tgacactctc gatcctgaag tagaacgacg ttaccgtaat
    11341 gcgctttcac tacaagcccc gtgtcctgac atcatccgca tcaatcgttt tgcgttttat
    11401 gaacgggcgc aaaaagcctt tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat
    11461 attcttttaa aaaaaggggt aacaccgtaa tctcataccg gtacgcccgc atgacgcggg
    11521 cggttatcga atgatggggt gaaaaatatg aaagcggcac gccagcaagc gatagtcgac
    11581 ctgctgctga accataccag cctgaccacg gaagctctct ctgaacagct aaaggtcagt
    11641 aaagaaacca ttcgtcgcga tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat
    11701 catggacgcg ctaaatatat ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc
    11761 aggctgaaaa gccattatgc gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt
    11821 gaagaaggga tggtgatagc cttagacgcc agttcaactt gctggtatct ggcacgccag
    11881 ttgcctgaca tcaacattca ggtcttcacc aatagccatc cgatttgcca tgaactcggt
    11941 aaacgcgaac gcattcaact gatcagttcc ggcggcacac ttgagcgcaa atatggctgt
    12001 tacgtcaatc cctcgctgat ttcccaactt aaatcgctgg aaatcgatct gtttattttt
    12061 tcttgtgaag ggatcgatag cagcggcgca ctgtgggact ccaatgcgat caacgctgat
    12121 tacaaatcga tgctattaaa acgtgccgcg caatcgttgt tattgattga taaaagtaaa
    12181 tttaatcgtt caggggaagc ccgcatcggg catctggatg aggtaacgca cattatttct
    12241 gatgagcgcc aggttgcaac ttctttggta acagcctgac ggaaagggta gcaggccgga
    12301 gacgacgccc cggccttgcc tgttatcgct cgtcgcgacg accacccacc gcagcccaga
    12361 tgcggcggac gtgcaccgtc acttcttcgc gatcgtgata caactgccgc gcctgaatct
    12421 gagcatttat gccatgttca tcaagctgtg cctgaatata cgccagattg tgtgatactt
    12481 cttcgtagcg ttttttcatc ggcagtttga gattgaaaat ggtttcacgg caccagccat
    12541 taaccagcca ctgcgccatc aatgccgcaa ctttagccgg tttttcaacc atatcgcata
    12601 ccatccagga gatattgctg cgtgtcggac ggaatttgaa accgtcttcc cgcagccacg
    12661 tcacctgccc ggtatccatc agactttgcg ccatcgggcc gttgtcgacg gaataaaccc
    12721 acatgttgcg cttcaccagt tggtaggtcc agccgccagg gcaagcgcct aaatccaccg
    12781 cccacatccc gttcgccagg cgttcatccc actcatccgc aggaataaac acatgaaatg
    12841 cctcttccag tttgagcgtg gaacgactcg gcgcctctgc cggaaatttc aggcgcggaa
    12901 tgcccatata gaacggcgaa ttattgttgc tgtatgagta accggtatag cagcatcctg
    12961 gtgcaataaa gaatacatgc acaaccggac gcttcggcgt ttcatagttc gccagcaccc
    13021 cagcatcgcg caaggcagcg cgtagcggaa cagtgaactt acggcagaat ttcagcaact
    13081 ctttgctttc gttggtatcg gcaacttcaa cacgcagttc accgcccttc tctactacac
    13141 cctgtaacat gccgacaatg ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt
    13201 cccccaccac aaaccactgg cgggcaaaaa ttaatgaact gaacggcagc tcacggatta
    13261 acttatcgcc atcatcaggt tgataacatt cataaatgac ataacccgca ttctctttca
    13321 cgcgggcaaa accgaaaatt tcccgctggc cggctttatc ggtaatttct gcggcgcact
    13381 ctttttcaaa gcccggacgg cacagcaata caaccttatt catgacttac gcccttgcgc
    13441 tttaaacgga tagcaccaac taacattaac gcccagcccg caaggaagct cacgccgcca
    13501 accggtgtga caaacgccca caaacgcaga tgggacagcg ccaggcaata aaggctgccg
    13561 ctgaacagca ccgtgcctaa cgcgaggaaa acgctactcc agtaaaacca gatactgatg
    13621 cgacgctgca ttgccaccgc cagacctaag atcgccagcg tatgaaacgc ctggtattcg
    13681 agaccggttt ggatccagcc catctcaacg gcccccatgg ttttacttaa cacatgcgca
    13741 ccaaaagcgc ccagtgccac aaaaatgaag ccgctaatgg cggcgaaaat cagcataaaa
    13801 cggctggtca tggtcgtacc ctacgtaaat tattgttcat aacgaaagcg gaatttttct
    13861 tgttcagcag cggctttcgc caggatccat tggcgaaagg cggctatttt acccagttct
    13921 gcctggctgt catgacaaac cagataaaaa gcatttttac tgaccagaac atcattaaac
    13981 gggcaaacaa gacgtccggc ctcgatttca gattgcgcca tcacgttatt tgccagcgcc
    14041 actccctgcc cgtgaatagc cgcttgcagc accatggcgc tatgactaaa aattggccct
    14101 tgctgaacgt tgatatgatt aaaccccaac tgtcgggtat atgtctgcca gtcacggcgt
    14161 gacgcatcat gtaataacgt atgtttagcc agatcttccg gggtcttcaa gggtttttcg
    14221 cctgtcagca gtagcggcga acacaccggc aataaatatt cggcgtacag tttttccacc
    14281 cgtagccccg gccagttgcc ccgaccataa aatatcgcca catcaacatc atccgccagc
    14341 ttatcttcct gacgatcaac cgcctggatt cgaacgtcaa ttcccggata agctgaatta
    14401 aagctggaaa gtcgcggaac caaccaatga atggcgaaac tggggagtaa actgaccgtc
    14461 aacgccccct tggcgctacg ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat
    14521 atctctttga tatcgaggaa atagctttgc ccttcctcgg tcagcaggag tgaacgatta
    14581 cggcggcgga acagttttag ccccaaaaaa tcctcaagag acttgatttg atgacttact
    14641 gcggcctggg tcacaaaaag ctcttctgct gcgcgagtaa aacttaaatg gcgtgctgcg
    14701 gcatcaaaaa ctcgtaaggc atttagcggt ggtaatcgtt tagacatggc ttttaaactt
    14761 tgatgttaaa tgaatttaac aattagatca cactgtgtaa cctattagtt tttttaatct
    14821 gagccattat aaattgtccg ttgagcttct accagcaaat acctatagtg gcggcacttc
    14881 ctgagccgga acgaaaagtt ttatcggaat gcgtattctg gtgaactttt ggcttacggt
    14941 tgtgatgttg tgttgttgtg tttgcaattg gtctgcgatt cagaccatgg tagcaaagct
    15001 accttttttc acttcctgta catttaccct gtctgtccat agtgattaat gtagcaccgc
    15061 ctaattgcgg tgcttttttt taccttgcga tcgcgaatta ctgttccagt tcgaccatct
    15121 ctttcacgtc agtacggttg atctgctgtt tgttgccatt agcgtcttta tacgaaatca
    15181 taccgatatc gttatcagtc tgtggtttgc cgtcagagac gatggtacgt ccgtcattgg
    15241 tgtgcatcac atagttcgaa ccggaacagg cgctcagggc aaaagtcagc atacaggcag
    15301 aaataattgc ggcagtcttt ttcatcatct tctccttaaa acgattaata aatcttaata
    15361 gtattgatta acaggctaaa attaacgcct aacactattc agcatatgtt acttacgcgg
    15421 cttcgccagg atatccagat aattctgatg gttagcactc tccttgtatc aaagtgaatt
    15481 ttgcgtcacg atcggtgcat caagccgagg agtaccatga acgtttttaa tcccgcgcag
    15541 tttcgcgccc agtttcccgc actacaggat gcgggcgtct atctcgacag cgccgcgacc
    15601 gcgcttaaac ctgaagccgt ggttgaagcc actcgacagt tttacagcct gagcgccgga
    15661 aacgtccatc gcagccagtt tgccgaagcc caacgcctga ccgcgcgtta tgaagctgca
    15721 cgagagaaag tggcgcaatt actgaatgca ccggatgata aaactatcgt ctggacgcgc
    15781 ggcaccactg aatccatcaa catggtggca caatgctatg cgcgtccgcg tctgcaaccg
    15841 ggcgatgaga ttattgtcag cgtggcagaa caccacgcta acctcgtccc ctggctgatg
    15901 gtcgcccaac aaactagagc caaagtggtg aaattgccgc ttaatgcgca gcgactgccg
    15961 gatgtcgatt tgttgccaga gctgattact ccccgtagtc ggattctggc gttgggtcag
    16021 atgtcgaacg tgactggcgg ttgcccggat ctggcgcgag cgattacctt tgctcattca
    16081 gccgggatgg tggtgatggt tgatggtgct caggggacgg tgcatttccc cgcggatgtt
    16141 cagcaactgg atatcgattt ctatgctttc tctggtcaca aactgtatgg cccgacgggt
    16201 atcggcgtgc tgtatggcaa atcagaactg ctggaagcga tgtcgccctg gctgggtggc
    16261 ggcaaaatga ttcacgaagt gagttttgac ggtttcacca ctcaatctgc gccgtggaaa
    16321 ctggaagcag gaacgccaaa tgtcgctggc gtcataggat taagcgcggc gctggaatgg
    16381 ctggcagatt acgatatcaa ccaggccgaa agctggagcc gtagcttagc aacgcttgca
    16441 gaagaagcgc tggcgaaacg tccaggcttt cgttcattcc gctgccagga ttccagcctg
    16501 ctggcctttg attttgctgg cgttcatcac agcgatatgg tgacgctact ggcggagtac
    16561 ggtattgcct tgcgggccgg gcaacattgc gctcagccgc tactggcaga attaggcgta
    16621 accggcacac tgcgcgcctc atttgcgcca tataatacaa agagtgatgt ggatgcgctg
    16681 gtgaatgccg ttgaccgcgc gctggaatta ttggtggatt aatgacaaac ccgcaattcg
    16741 ccggacatcc gttcggcaca accgtaaccg cagaaacgtt acgcaatacc ttcgcaccgt
    16801 tgtcgcaatg ggaagataaa tatcgccagt tgatcatgct ggggaaacag cttccggctt
    16861 tgccagacga gttaaaagcg caggctaaag agattgccgg atgcgaaaac cgcgtctggc
    16921 tgggatatac cgtggctgaa aacggcaaaa tgcacttctt tggcaacagc gaagggcgca
    16981 ttgtgcgcgg cctgttggcg gtgttgttga ctgccgttga ggggaaaacc gccgccgaat
    17041 tacaggcaca gtcgccgctg gcattgtttg atgagctggg attacgtgcg cagcttagcg
    17101 cctcacgcag ccaggggtta aatgcgttaa gcgaggcgat tatcgctgcg acgaagcagg
    17161 tttaagcaca gaaccgggtc ggataagacg ttcgcgtctc atccgacctg attgtttaac
    17221 cctgacgcgc cgctttcgcc atcatcttct tcagcgcatg agaaaccgca acaaacccaa
    17281 aggtggcggt caccatcgtt gccgcgccaa atccagatgc acaatccatc cgcttcggcc
    17341 cttctgccgt ggctttcatc gcacacaccg taccgtctga ctgcgggtac accagcgctt
    17401 cagtagaaaa cacgcaatcc acgccgagct tacctttact gtttttcact acgccaaaat
    17461 cgcttttcag gcgctcgcgc aacttcgccg ccagcggatc ctgaatcgtt ttcgccagat
    17521 cggtaacctg aatctgcgtc gggtcaatct gcccacccgc gccaccggtc gtgaccagcg
    17581 ggattttatt gcgccgacaa taagcaatca gcgccgcttt gggccgtaca ctatcaatgg
    17641 catcaatcac gtacgaataa ccggcactca tatattgcgc tacgttatcc ggcgtcacga
    17701 aatcatccac caccgttaca cggcactccg ggttaatctg gcgaatgcgc tccgccatga
    17761 cttccgcttt tgccagcccg acgttatcgc gcagagcgtg aatttgccga ttggtattgg
    17821 tgacgcacac atcatccata tcgataagcg tgattgcgcc aatccccgtg cgcgccagcg
    17881 cttccgccgc ccaggaaccg acaccaccga taccaaccac acaaatatgc gcgtcagcaa
    17941 acagttgcaa cgctttttca ccatacagac gcgctgtgcc accaaaacgc tgacgccatg
    18001 catcactaat taccacagac atagcacctc agaattaaaa agggcgaggt tatccctcac
    18061 cctaaacgca taaatgcctg atgcgctacg cttatcaggc ctacataact cctgcaatat
    18121 tttggatttg catgatttgt aggccggata aggcgttcac gccgcatccg gtatgaacaa
    18181 cgcgcactta gtcaataatc aggatgtgag gttacccctc ccctgtcata tccgtaaaaa
    18241 cggcatacag aatatcacat cagccgctaa agacgttacc tgcgcccgga gccgttttca
    18301 gcacccaaac ccgtccatag tggttgtacc aacctgcgcg gtgtccggct tccggcccga
    18361 tcccttgata gatatcgaag tgttggcctt tgattgcgcc cccgacatcc aacgccacca
    18421 tcagacgcaa ttcgtactga ccgttaaatt tgccgttatt atccagcagc ggcacttctg
    18481 ccagcaaggt agtacctggc ggaataattg aacgatcgga ggcaacagaa gcacggccaa
    18541 ccagcggcac cgcacttgcc cctttcaccg gtgcaaaaga ttgcggttta aagaagacga
    18601 aagacgggtt ctgttccagc agctcgcgaa cctcggcttc actgtgtgtt tcgccccaat
    18661 gacgaatcgc ctgcatcgac atatcttctt ttttcacttc gccacggtcg atcagcacct
    18721 taccaatgct gcgataggca tgaccgtttt tccctgcata gctgaaaaag ttaagcggac
    18781 tgccatcacc aaagtcgata tacccactac cctgcacatc cataatgaag ttatccatca
    18841 gagagttgct gtaagcgagg atgtatttat cgcccaatgc cccggcgtag atctccgcac
    18901 gagacggcag acgaccacgt tttggcggca tacggtaaat aggatactgg aactcgccct
    18961 ggcgggtatg gcgcgcctga attaccggcg tgtaatagcc tgtaaactgc acgttaccat
    19021 agttgtcggc accttccatc tgccaggcat caatgccgaa ctggcgcata ttgcgggtat
    19081 caccgcctgc gcgcagccac tcttgcaccg cgttataaac attactctgg ttgccataca
    19141 gacgcggtga cgaattacgg atatggttaa tttgctcggc aaaatcaccg gcgttaatcg
    19201 gcgcgccaac ggcatctggc tggttcacca gagagaaagg ctgggtaaat ttcccgtctt
    19261 tatattgctg tccgcgatcg gttggtttgg aagagcaggc ggcaagcatt gccacaaccg
    19321 tgcccataag aaggtacttt acccaacgtc ctttcattgt tctcttcttc aggttaaaaa
    19381 ataaggcgca acgaagataa caaaccgccg cgtttaatga aatgagcagc ctctccctga
    19441 cgcaaatttt gcacaaaaaa taggctttag tggtttgttt ttgttcaaaa tcatgctaaa
    19501 tccgtgatcg gggtaaaaaa aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc
    19561 acggacgcgg ggtggagcag cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt
    19621 tcaaatccgg cccccgcaac caatcaaatt tgatgaagta aaagcagtac ggtgacgcgg
    19681 ggtggagcag cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg
    19741 cccccgcaac caattattga acaccctaac gggtgttttt ttgtttctga tctcccataa
    19801 aaaaagcgcc attcagcgcc tttttatcat ccccttctcg ccagcgttgc cccatcggca
    19861 aaatacgctt taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt
    19921 ttcagtttac gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga
    19981 atatctggtg cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg
    20041 ttgatttttc cgagcttatt cagtaccgct ttaccaaact taaggctgtc agcaatagtc
    20101 agcgactgca ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg
    20161 ccaccaatca agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt
    20221 gcgcctttgg ttgagagcgc aaacaccgag gaaccgctcg gctgacgact ggtaaaggcg
    20281 tcggcatgga tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact
    20341 tgcaacggaa tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctcttcc
    20401 tcgatcagag agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg
    20461 tatttcccca ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc
    20521 ggacgatcgc gccctgcttt acccggttgt ggaccacttt gcgctggcgg cacctgcttt
    20581 tcgaggtcgc ctttgttgta atcctccagc agcgccagca gcgggtcctg catatcctgt
    20641 gcattggccg gatagaggtc catcaccaga cgctctttaa acccggcgac cggcgcaagg
    20701 gcaaacagct gcggttttac gttttgcttt aattcaaaaa ccatgcgtac ggtttgcggg
    20761 tcaaattgcc cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc
    20821 atccccttga gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttcaggg
    20881 ttactcaacg cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg
    20941 tagctggacg caggccagac gcgcaccgcc acgacctggc taaccgcagc caggctgacc
    21001 tgacttacgc tcaatagcca catagcgccc gcgccttgca gtaaacgacg acggctgatt
    21061 gcagtgttgg atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt
    21121 aaacgactaa tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg
    21181 gtaaacattc tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt
    21241 tacacttgca ccctgcgaaa aaacagaata aaaatacaat aatttcaaat aatcatgcaa
    21301 aggggtgtgc cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg
    21361 ttccctatat caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca
    21421 ttgagcatga gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca
    21481 tccgtctggt ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc
    21541 accacgaacc gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg
    21601 tgaagcaggc tgcgggaaca ttgcaactgg atattactgc tcgcctgtca atgagtctca
    21661 ataacacgcc gctgcagggc gcgcatatca acgtcgttag tggcaatttt attattgccc
    21721 agccgctggg cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg
    21781 atgaagacgc gctccatcgt caactggaaa gcggtgcaat agtgctaatg gggccggtcg
    21841 ctgtttcagt cactggcgag agctttaatc tgacctcgga agagattgcc actcaactag
    21901 ccatcaaact gaaagctgaa aagatgattg gtttttgctc ttcccagggc gttactaatg
    21961 acgacggtga tattgtctcc gaacttttcc ctaacgaagc gcaagcacgg gtagaagccc
    22021 aggaagagaa aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag
    22081 cctgccgcag cggcgtgcgc cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt
    22141 tgcaagagtt gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc
    22201 agattcgtcg cgcaacaatc aacgatattg gcggtattct ggagttgatt cgcccgctgg
    22261 agcagcaagg tattctggtg cgccgttctc gcgagcagct ggagatggaa atcgacaaat
    22321 tcaccattat tcagcgcgat aacacgacta ttgcctgcgc cgcgctctat ccattcccgg
    22381 aagagaagat tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agttcatcac
    22441 ggggcgaggt tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat
    22501 tgtttgtgct gaccacgcgc agtattcact ggttccagga acgtggattt accccggtgg
    22561 atattgattt actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag
    22621 tgttgatggc ggatttaggg taagcgatga aaatcaccgg atgcggcagg cgtaacactc
    22681 gtgcgtcgca tccggcaatt acgtttattc ccgcgaacta aacaacgccg ccagaccact
    22741 gcgccgctca gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga
    22801 cagacgacga cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctaccggcgt
    22861 gcgttggctc ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac
    22921 cgtcatcgcc cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt
    22981 gccgtccggc atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat
    23041 atcgccatta aacaacccaa gcgcgctgtc attacgggca atcatcaccg gtcggccttc
    23101 gtaccaacga gagtgcggat gacgatgaat tttgcgcttc tgttgcataa actgttcaat
    23161 tcgctcattc agtccagcca cgccaaacgg cccttcccgc agggcgcaca aaagctggta
    23221 ctcattgaac gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata
    23281 acgtccgtaa cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa
    23341 aagtcgtttt tcgatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc
    23401 accacggttg atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct
    23461 tttttgcagc aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg
    23521 aacgtgagtc cccgtcaggc ggcttagctg cctggcacgc tcggcggtaa agcccgcgtt
    23581 ggcataagcg cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg
    23641 atcgccgaga aagatcactc gcgcatgatc tggcaaggcg tcgatcagtc tcgacatcat
    23701 aggcagatcg atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc
    23761 ggcatgatga cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgct
    23821 ggcatcttcc ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt
    23881 gccgagagat tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg
    23941 gcagcgttcg ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt
    24001 tttaccggtg cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgccgc
    24061 tgcaactttt tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc
    24121 cagtagagct tcatcaacct caatggtatg attcacctca ttgaagaagc gcgctaccgt
    24181 ccgctcgtta caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt
    24241 gggttcatct cccctgctga ccgcttgaga agccagcaag cattcttccc aattttgtag
    24301 ctcaccgatt tcactgacac aggtcgccaa cagcggattc gacgcctcgt tattttccag
    24361 tcgtgaaagc ggcaaacaga cgtgtccctc tccggcatca tgacttaaca gtgccgccgc
    24421 gagggtgacg gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg
    24481 gcgtagctgt ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc
    24541 tccagggtca taccggcaaa catctcatcc atcagggcaa tcaacccggc gttgggtcgg
    24601 gttgtgtaaa tcccttgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata
    24661 acgccgccaa agtgaagatc atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc
    24721 agcgccaggg tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc
    24781 atagcctgtt gggtgtaagc cgaactgtct tcacccagcc agttggattt atagtcgagc
    24841 aggtaataac gcccttcgtg gcggaatacc aggtcgataa agccttttaa catgccgcgt
    24901 acctgcatga actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt
    24961 gcatcaagct ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc
    25021 tgtttatcgc ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg
    25081 aggacagccg tgatccactc ggtcaacacc ggttcccact gcgattcaaa gccgccgagt
    25141 tccagttttt cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac
    25201 aaactgtgca agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc
    25261 ggttcttcaa cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc
    25321 tgagcgatac cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg ccagttatcg
    25381 ccgggcaatc gttgtagtgt cctcgcattc agttctgcag ttaaagcatc attaacctgc
    25441 cagggctggt tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct
    25501 tcaatacagg tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg
    25561 agcgcacttt ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt
    25621 gcaacgccga gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga
    25681 tcttccgcca gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc
    25741 aaaactgcct caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg
    25801 gtaataaatg gcaaccagac cagcggatat tccagccctt tcgatttatg gatcgtgaca
    25861 atctgcacca gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct
    25921 ggctcgagga tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt
    25981 ccggcttctt gaagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct
    26041 gccgttgcca gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc
    26101 ataacgccac gtttgcgcca gatttgccga taaccatcga actcttcgac taccgcatcc
    26161 cacgcatgtt cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac
    26221 gttgccagcg cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacaac
    26281 caaagcattt cctgcgcttc cagagtttca aaaacactgt cgcggttcga aaggtaaacg
    26341 gaagggattt ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc
    26401 accagcacgc tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac
    26461 gcttcgcccc gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc
    26521 atggtacttt gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc
    26581 gcaggctgcg tttcaccttt aaatacaaaa cgtaacgcct gatttttccc ggctgatttc
    26641 actggaataa acggtatttc gcgaaacatg aacgtgtcat cagtctggct gaaaagcttg
    26701 ttcacactgt tcaccattcc aggtgcggag cgccagttgg tgtctaaagt gtagtgggca
    26761 tgaacttcac tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg
    26821 gcctgcttcg ggtcgccaat cagcaacaat gcggtttccg gctgatggtg ccagatacgg
    26881 cgaaaaattc ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg
    26941 aatcgcgtac ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg
    27001 agccgactta acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact
    27061 gtttcgcgga tctcagccaa tgcgcgggtg atcaacagat cgcggatcga caatggttct
    27121 gcaagcagtt gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggccttc
    27181 gtgcgatctt ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg
    27241 tttgtctctt cttctgccca ggcgctgatc ttttcgatcc atttagcctg attgctacgg
    27301 ttaaacttgc gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg
    27361 tcgcgccact gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg gcgggaagcc
    27421 agcgtttcat catcgggcgg cggtgctttg ataaccggcg gttcgccttg cagataacga
    27481 ttaatatcgc gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt
    27541 tcacgcggca gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc
    27601 agagactcat cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg
    27661 ttgagcatgc gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt
    27721 tcggctaaca acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggcgt
    27781 tcgtacagtg gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg
    27841 ctacggatac gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt
    27901 tcttcaacga tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc
    27961 aaatagagcg ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc
    28021 tcgccctgta agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc
    28081 actcatcagg ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc
    28141 aggatgcaca tattcggctt tcccgttctg gctgccggaa atctgcgaca gaatagccat
    28201 gccttgcggc tcgaccaccg cctgatggaa gaaatcagca agtttttgcg gcgtcagcag
    28261 ttttatctgg gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc
    28321 tttacttaac ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggtaat
    28381 taccgcctgc tggatttgcg caaactcatc tggcttcatc gctcgcaatt ttgcctctgc
    28441 ggttgggaaa aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg
    28501 caaaaggaag cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata
    28561 gcccaattgt tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa
    28621 cagagagcta taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc
    28681 cagtgcggag tcggtgctgt taccggcttt ttcaaagatg acggattgtt ttttatcgac
    28741 cactacatct ttgtttcgac accactctga accatcagcg cccaactgtt tttgcacatc
    28801 gcgtgccagc gttgttgcct gggcctcggt catgttgccg ataaccataa actctggtcg
    28861 agcccctgat tttaaggcgt cgcgataggc cagcacctct ttcaacgtaa tggagggcaa
    28921 aattttacgc cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat
    28981 aatcgcctgc tcaaacgctt tacccttttc tgcggaatcc atcatctggt tataccagga
    29041 cttcgcctgc tcaagctgct cttccgtagc ggtatagcta aagtacccct cgagcaatgc
    29101 ctggaacagc tgcggcagac gctgggtgta accattagca ttaatcataa ggccgttgtt
    29161 agcgttggtg gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc
    29221 cagccctgcg agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc
    29281 tttcggatta cgcaaaatca ggctgacatc agctttgggc tcgctggcaa aataacggct
    29341 tggcgcatac accacgcgca gattcgactc atcaacaatc agctctggat ggtcgtattt
    29401 cttctctgac ttaatcagcg agaaatcatc aggaatataa gggttaagct ctggcaaaga
    29461 gagcgcaatg tcggcggctt ttttctgcca gtcggcgaaa gtttgtgcgc tgattttatc
    29521 gacctgatac ggcgcatcga caaagtaagc cgttttgttg tgcggctctt tcgggctgat
    29581 ataccagata cgcgcattct gcggcgtcat catcgccaga cgttccttta ctgctttagc
    29641 atcgtaccga tcggcaatat tgactgcatc cagcgtatgc tcaacaggaa cgcgaatcat
    29701 ggtatctgcc agccattcga cgtaatccat atcacgggtg atcgacggat aacggaagtc
    29761 gatatccagc acattcgcca gttcatcgaa gtattgttta tcaatgcctt tttcacgtaa
    29821 cagattgaga tagctaaaaa ttgccgccac aacctgatcg cgattagcca ggcctttatc
    29881 agttaaagac gcagagatcg ctaatacgcc gctgttgccg ttgacgatag gatcggagtt
    29941 ggcgctaatg ccctcaacta atccctgctt ttgcagccag tcagaaagtg tacctgggct
    30001 gcgattgcca atcagatagg taatcaattc atcggtttta ctacggaact tcgctgagtt
    30061 gttatcgatg cgaaactcaa cgcgcaacac tttacgcggc agcgcaggga cgtaatgaat
    30121 gataatgccc ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc
    30181 tttgttcggc acgcgaccaa aggtgtccgc cgccattttt gccaactccg gcagcggttt
    30241 attactgtaa ataaccgcct tcatcaaatt ggcggaatag tacttctcgt ggaaatcttt
    30301 cagcgcctgc tgcaccggat taccaggttt gtcgcttaaa gtttcgaggt taccaccaga
    30361 aaactttgaa ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat
    30421 cccgtcacgc gtacgcgcca tggttaattc agcgttcact gcattacgct cacgttcggc
    30481 gtatttcttg tcgagcaagg gttctgcaat agcatcggcc aggcggtcta ccgcaccggg
    30541 caaggcgtcg ttctcaactt ccagatagaa agccgtgcga tacggcgcag tgctggcatt
    30601 gtgactaccg ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt
    30661 cgaccccatc agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc
    30721 ttccagcgac ccaacgggca ctaccagcgc cgagagcgat ttaactgcct gcggatcaga
    30781 aaccagcaag accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt
    30841 atcactttta cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaatgg
    30901 tgcccagagg gcaacaaaca acaataatgc tttgaaccag gtgctgcggg gcattcacgg
    30961 acctcataat caactttatt ttctggacag attcaacagg ttaacgcaaa tcatctgcca
    31021 gaatttaatc ttgtgctgca cgagccagcc tatgtttata taaccatcag tccgtgactg
    31081 gtgcgcatca taaagtaagc agatagattg cgcaattttt atacaacact catgactgat
    31141 taaagcgaaa cagcggtaac aggaaacgtt gcgactgttc aacgatggcc tccattgtct
    31201 ctggtgttaa ttgccgccag agccgttgat accagatatc atcaccttcg ccacgcacca
    31261 tcatgttgcc ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt
    31321 catccagcat ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt
    31381 caggtaacac cagcaatggc gcggacattc cttcacgata cccctcaatc agttgtgaga
    31441 ggtaatgcaa agcctgttcg gctgcaagcg gcggaaaacg ccactcaccg tctttgcgta
    31501 gaaaaaggcg actttcgcca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa
    31561 gttgcattcc ctgcgccaca cttaataaag agggacgcca gcgcaacagg ccatccggct
    31621 gcacctgcgg caaccagcca gttatctgca caccgttgca ggcgagatca atttccatac
    31681 tctgccccgg ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctggcact
    31741 gtgtttccca gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc
    31801 ggcggaacaa gcgttcggca tcatcctgct caaccagtgc attcaataac tgctgattga
    31861 tttgataacg gctaagtcct tccagaataa atggctcggt gtctgggatt tcgctgtctt
    31921 cagtatggaa gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga tgtgcccaga
    31981 atcgttgtag cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa
    32041 attcagaatg tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt
    32101 gtcgttcgcc tggctggtag ttttgcggat caaacggcat ccgggtatgg aggcaagtaa
    32161 gatgcgcttt tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat
    32221 aatgactttg cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac
    32281 tgttatcctg aatggaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta
    32341 acgcttccag gaacaggtag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt
    32401 tctggctcat caggtcaaag cccaatggcg caagctgacg tggataaacg ccgtcgttca
    32461 ttcccagcag gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt
    32521 taaccggtcc ggcgagaaaa cgctggctga tacgttcttg atccagacgc tgtgccagtt
    32581 catcacgcaa tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga
    32641 taatcgcctg ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca
    32701 ggaagaaggc gttgagcata tcgcgacaaa ccggcaacca ctcttctagc ggacgctcct
    32761 gtgccagccc gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca
    32821 gttctgcaat taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg
    32881 cgctctccat cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc
    32941 cggtggcggg gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg
    33001 attcgttgac ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg
    33061 ccagcaccgg cacatccagc agcgccagca catcctctga cacaaagcga ctgtcaggca
    33121 gtgataacag gctgataaac gcttccagta ccggatgtga ctgccgcgca cgacggtcgg
    33181 aaatggcgta aggcaggtaa cgatccgcag gtgcgctacc aaacactgcc tgaataaacg
    33241 gactgtagct gtcgatatca gccaccatca cgatgatgtc gcgcggagta agtgtcgggg
    33301 cttcctccag catcgccagc aagcgatcgt gtaaaacttc aacttcacgc tgcgggctat
    33361 ggcaaacgtg gaatgtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac
    33421 gggaaaactc ttcgatgttc acacccgcaa cggcgcggtt ttccagttcc agaatgtcag
    33481 actgaatgtt atgcagcagg ttatctggcg tcacatcgac aaaagcgtcc agctcctggc
    33541 tgctctccag gtcagaaagg agataaatgt agtcgcgccc aagcttcccc catgaggcca
    33601 gcagcgagtt gccgacatcc tgttcaccat cgctgttaaa gagctgtccg gcattttcgc
    33661 tatcgcgaaa tagcggcaat tcgcgatctt caaaactgtg tcgacgctga cgagtcagca
    33721 gtttcgccag ataagcagga tctttaatat cgccccagta ataacggcag gggttggtaa
    33781 acaggagatg gatttcaata tgtttaccca gcgcctggag cgcctggaga taaacaggcg
    33841 gtaacgcgga aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg
    33901 cggactccag cgtttcgata aagcgctgat agagattcgc gcggtgccag cgcggttgtc
    33961 cgagttcatg ggtatattcc accagcgcct tccacaacgg ggcttgccag gcctgtgctt
    34021 ctcccagccc ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc ggacgataga
    34081 ccagatactg gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt
    34141 cgctatcgtc agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc agcaattgcg
    34201 gcagcagagt catcagtttc cagctcatgc tctgtttgtt aaaggcgctc tctttgggga
    34261 tttccggtaa cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa
    34321 tgtttgccgc aataccaaac ttttgcgaca gggtcatttg cagccactgc gccataccgg
    34381 tactttgcac cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa
    34441 taaactccat caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag
    34501 cggctcctga ctactgacaa ttcgggcaat gcaggcgggt catctcgcct tctctgcccc
    34561 cgggtgaaac tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt
    34621 tgacctgcca gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc
    34681 gccagagctt ttggtactgg tttctgctgg cgagactgtt cattgatgtt cgctggatac
    34741 ccgataacgc agtgacaatc atcaccatca gcaccatcag caccatcagc accatcagca
    34801 ccatcgcaac cattacctcc ggcaggctaa agccttgttg attcttcagg gaagctgaca
    34861 taatgccccc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa aaacaatttt
    34921 tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg caatcaataa
    34981 ggcttcatta tctgccagta aacgcaaaca aacctgggca tcggttccag cgtactgcga
    35041 gcactgaact gctggctgcg tctgccagga gtgcattttt ccccacgcca gcgccgactg
    35101 aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagagg caaaactgcg
    35161 atcctgctga ctcattcctt gcaatagcaa gctacccaaa accagcagca tcaggaccag
    35221 agccagtgac gaaacacctt tttcgcggtt cacaggttga atcctgtcac gctatagctg
    35281 gcatccacca cggtttgcgg ttcagactta ctggcagcac gcatattaac cgtcaacacc
    35341 ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat gatggcatcc
    35401 ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg tagcgtttcc
    35461 agcacatgct ccttcagacg aaatccaatc tggtcggact cttttaccgg ttcgcgatcc
    35521 cagataccgt tactgttcgc atcccactgc acaatgatac agtcaccctg tccgacaatt
    35581 tccagccctt cgccggtaca gctgccatga caataacccg ccctctggag atgcttcgcg
    35641 acggtaaata cccgcagcca gatttcatct tccagtgcca gcttacgagt gctcgttaaa
    35701 ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac gctactgatc
    35761 gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg catccttctg
    35821 tttctccttg ctgataaagc cggagtcttc cccacggcga aaccaccagc caccactcgc
    35881 ccgttgagtt tttgaagcga atatgcacgg cccatgcggt attgcgcagg ccaaagaaag
    35941 caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca aagaccaatg
    36001 gtgaactgcc atgacaggta ttggccccag cagcggaact cacaaggcac cataacgtcc
    36061 cctccctgat aacgctgata ctgtggtcgc ggttatgcca gttggcatct tcacgtaaat
    36121 agagcaaata gtcccgcgcc tagctggcgg tttgccaaag ccgttgcgac tgctgccagt
    36181 attgccagcc atagagtcca cttgcgctta gcatgaccaa aatcagcatc gcgaccagcg
    36241 tttcaatcag cgtataacca cgttgtgttt tcatgccggc agtatggagc gaggagaaaa
    36301 aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta cgccgttgca
    36361 ggacgttgca aaatttcggg aaggcgtctc gaagaattta acggagggta aaaaaaccga
    36421 cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc ggcgctttaa
    36481 cgcccggatg tggatcgtag ccttcaatct caaagtcttc gaaacggtag tcgaagatgg
    36541 attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg cttaattgca
    36601 gatgagtttg atccatatgg ttgctgtaca ggtgcgtatc gccaccggtc cagacaaaat
    36661 cacccacttc cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg tagctggcaa
    36721 tgttgaacgg caggccgagg aagacgtcac aggagcgctg ataaagctgg caagagagtt
    36781 tgccgtctgc cacatagaac tggaagaatg catggcacgg tgccagcgcc attttatcca
    36841 gttcgcctac gttccacgct gaaacaataa tgcggcgcga atccgggtcg tttttcagct
    36901 ggttcagtac cgtagtaatc tggtcaatat gacgaccatc tggcgttggc caggcgcgcc
    36961 actgtttacc atacactggc ccgaggtcgc cattttcatc ggcccattcg tcccagatgg
    37021 tgacattgtt ttcgtgtaga taagcaatgt tagtatcgcc ctgcaggaac cacagcagtt
    37081 cgtggatgat ggaacgcagg tggcaacgtt tggttgtcac cagcgggaat ccatcctgca
    37141 ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta cggtcgtttt
    37201 tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc atggttcctc
    37261 aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc acacccgcga
    37321 caatcatcgg gatggaaaga atttgcccca tgctgatgta ctgcacccag gcaccggtaa
    37381 actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg tagccaatca
    37441 ggaacaagcc tgagacagct cccattgggc gcggtttacg aatatacagg ttgaggataa
    37501 taaacagcac cacaccttcc agcagcagtt cgtaaagctg tgatgggtgg cgcggcagca
    37561 caccgtaagt gtcgaaaatg gattgccact gcgggttggt ttgcagcagc aaaatatctt
    37621 ctgtacggga gcctgggaac agcatggcaa acgggaagtt cgggtcaacg cggccccaca
    37681 attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga atgagtggtg
    37741 cgataaaatc agagacctgg aagaaggaac gtttagtacg gcgggcgaag ataatcatca
    37801 ccacgataac gccaatcagg ccgccgtgga aagacatgcc gccgtcccag acacggaaca
    37861 gatacagcgg atcggccata aactgcggga aattgtagaa cagaacataa ccaatacgtc
    37921 ccccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca tttttggtcc
    37981 agccactgcc cggacgattc gcccgacgtg ttgccagcca cattgcaaaa atgaacccca
    38041 ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa atgaccggat
    38101 caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc ttgttatttc
    38161 gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc aaaggtgcga
    38221 ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat gtatatgatc
    38281 cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata aaggctgcaa
    38341 cctgatgacg aacttcggtc gccagttgcg cttccagact acgctgcgca agattttctg
    38401 cttcggcaaa atcaatgcgc cgcagcaggt attttacccg cgctacagaa cgtccgttca
    38461 tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga tcgcccgcca
    38521 tttcaccgca caaacgaaga tcgataccat gtatttccgc ttcccgggcg atcatcgcca
    38581 gagctcgtaa cattgcagga tgaagactgt cgtaaatgtt cgccacccgg gtattgttgc
    38641 gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg aaatcgaccc
    38701 tctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg atgccgatac
    38761 gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca cgttcaatca
    38821 ggcggcgtgc ttcgtcaact tcatcgaggc tggtgaccat cggcaacaga atattcaggt
    38881 tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag atctccggct
    38941 gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg ctgatcggca
    39001 tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg ggtttatcat
    39061 tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac gggaaaccac
    39121 tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata cagctgccca
    39181 gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata cgctcaccgc
    39241 tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc tcaatctctt
    39301 cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc agcaattcac
    39361 cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga atatccgcgc
    39421 ccatcacggt agggatcccc agcgcacgta ccatgatcgc agcatgggag ttggctgcgc
    39481 catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga gttgtcgctg
    39541 acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct tgattagcgt
    39601 catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcaccagcc cgctctttga
    39661 gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc gtttttaccg
    39721 cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc agccgggtgt
    39781 ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt gctccggcgg
    39841 caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc ccggtcagtc
    39901 gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc attaaaggta
    39961 acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt aatgcgcgga
    40021 ttcgcgtctg gcgatattgc ccaaacaggg cagtcaactg cgactgagaa agaatagctg
    40081 ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac tggcgcaact
    40141 ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt ggtacgccta
    40201 aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg gggtgctttt
    40261 gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg atcccttcat
    40321 caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc gccatcaggt
    40381 agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta tccatcgcaa
    40441 gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg ctggctacct
    40501 tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct cttttacgtc
    40561 gataagcaga tgcgttttgt ggttttggcg tattttcctg cagtgacatc accacactcg
    40621 cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc tgtctgaccg
    40681 gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg gtttgcatat
    40741 tgatttctgc atcgccgctt accagctgca agagaaacca tttttgtttt tggccgatac
    40801 aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc aaccagttac
    40861 gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca aacaattcac
    40921 ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga aattgccagg
    40981 agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa atcacgatac
    41041 ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat aaagctttat
    41101 atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca gggcattaca
    41161 cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga ggaaggggtg
    41221 gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac ggaattacta
    41281 cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat attcattatt
    41341 agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac ccaggtcaca
    41401 cctctcattt acgggttggc gaaagatcaa ccaatgccgt gtttatccac agaatgtgcc
    41461 actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa ggctgtaaat
    41521 gaaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa catggtgtaa
    41581 gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgttg cgcaactcga
    41641 ttaccggcag cctaaaaagc aatataaatc agagaattga acaacacatg tggaaaaagt
    41701 tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt atcatgacat
    41761 gtcccaacct cgcccactgc tctctcctcc cgaaactgaa gaacagttgt tagcgcaagc
    41821 acagcaactt tctggttata cattgggaga actggcggca cttgacgggc tggttacgcc
    41881 agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct ggctaggtgc
    41941 cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac ttaaaactat
    42001 ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg cccggttaac
    42061 gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc cacaagctca aacgcgtact
    42121 gtggataccg gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg taggatcacc
    42181 gttgctgtgg agcccgaatg aagaggaaga ccgacagcta cgcgaagact gggaagaatt
    42241 aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg gggaatattt
    42301 acagataaga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg gtgcccgggg
    42361 cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca ccagtgcact
    42421 actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt tccgggcata
    42481 taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg gataaccgat
    42541 cctaacgctt ggcttgcgct cggtacgctg acgctgctgg agatcgttct tgggatcgac
    42601 aatattattt tcctttctct ggtggtggca aagcttccca cagcacaacg tgctcatgcg
    42661 cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc atcaatctct
    42721 tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat ttctgcccgt
    42781 gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa ggaaatccac
    42841 gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt cctcggcgct
    42901 atcgtgcaga ttatgctgct ggatattatc ttcagcctcg actcggtgat taccgctgtg
    42961 ggtctgtcag atcacctgtt tattatgatg gcagccgtgg tgattgccgt aggcgtgatg
    43021 atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt aaaaatgctg
    43081 gcgctctctt tcctgattct ggtgggcttt accctgattc tggaaagttt cgacatccac
    43141 gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga aagcctcaac
    43201 ttgattcgca acaaaaagaa tccgctctga tactccgtac gctctcctgc gggagggcgt
    43261 actcactcct tttaccctca cagtttaaga ttttcctgct ttcaagatat ataacgtcgg
    43321 tttataaaca gactattatc ataggtaggc caaatgagga taaccggatg aaaaaatggg
    43381 cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc agtgattacg
    43441 tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa attgatgatg
    43501 ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt aaccgtgatg
    43561 atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgg ccttaagatt
    43621 tttctctttc ccttttccct tccctctgcc atttttatat tccttatgtc gtgattataa
    43681 aaaggaaacg gctatgcaat atcaccgtat accccacagt tcgctggaag tcagcacgct
    43741 ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc acgcacaact
    43801 cgactatgcc gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt acccagtacc
    43861 tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc ggcaactggc tggcgaaaca
    43921 tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc gcaataatga
    43981 caagggcatc cgcccggatc aggcgctgga tcggaagaat atccgcgaag cgctgcatga
    44041 cagcctcaag cgcctacaga ctgattacct cgatctttat caggtgcact ggccgcagcg
    44101 cccgaccaac tgcttcggca aactcggtta tagctggacg gattctgcgc ctgcggtttc
    44161 gctgctggat acgctggacg cactggcaga gtaccaacgc gcgggaaaaa ttcgttatat
    44221 cggcgtgtcg aacgaaactg catttggcgt aatgcgctac ctgcatctgg cggacaaaca
    44281 cgatctgccg cgtattgtca ccattcagaa cccttacagt ctgttaaacc gcagttttga
    44341 agtaggtctg gcagaagtca gccagtatga aggggtcgaa ctgctggcct attcgtgcct
    44401 gggtttcggc acgctgaccg ggaaatatct caatggtgca aaacccgctg gcgcacgtaa
    44461 tacgctcttt agtcggttca cccgctatag cggtgagcaa acgcaaaaag ccgtcgcggc
    44521 gtatgttgat atcgccagac gtcatggcct tgatcctgct cagatggcgc tcgcgtttgt
    44581 acgccgtcaa ccgtttgttg ccagcactct gctgggcgca accacgatgg atcagctgaa
    44641 aactaacatc gaaagtttgc atctggagtt aagcgaagac gtattagctg aaattgaagc
    44701 ggtacatcag gtttatactt atcctgcacc ataaagatta acgccggtta aaaccggcgt
    44761 taaatattaa tgacggcgct gccagatcca cagcgccgtt attgccagcg caaatagcgc
    44821 accgaagcca atgccaatgg gcacgaccgg gatgcctacc attaccgcca gcgagtaaat
    44881 gcccagcatc aacaacatgg cgctgttttc gccaaggttt tgtactgcaa tcgcattccc
    44941 cgccccgacg ctttttttac cccgctcctg tagcaacgca ttgagcggaa cgacaaaaaa
    45001 gccccccagc acgccaatca gcatcaacaa ggcataggct ggcagcagct cgtgttgcag
    45061 ggagaaaatc ggtaccacca cgccaatcaa aatcccggct ggcatacagc gtgacacggt
    45121 ttccagcgta accagcttcg ctgccgcacc tgcgccaacc acgataccaa tcgctaccat
    45181 cgcgttgaga taggtcggcg tagcgttatc ggtaatgccc agcgccaccg gtacccacag
    45241 caccaacagg aaacgcagcg tgacacccgc tccccagaat aaactggtgc ccaccagcga
    45301 aaaacgcgtt tcaccattgc accatagcga ggtgcaggca ttcaggaaac tgcgggtcat
    45361 gttgatgaga ttccaggact gccccggacg cgccgccagt ttgggaatgt agatattggc
    45421 aacgaccgca ccaccgtagg ccagtgcgca tgcggccaga gcgaccagga catgccagtc
    45481 agccagcaca ccaccggcta cggaaccgag taaaatcgcc gctatggcag aagcttccat
    45541 taaaccgtta gcttttacta acttactacc cgtggttaat tcgccgagaa taccgtattt
    45601 cgccggtgaa taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat
    45661 accaaagcag atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg
    45721 gcctttggcg aagctatccg ccacctgccc gacaaacggc gccaaaagaa tgtaagcacc
    45781 tacaaacacc atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa
    45841 cgccagagtg gcaaacagta gcgcgttatc gccaaacgca gagagaaact gcgccacgat
    45901 aaccgctttc atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt
    45961 gttgttccgc ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa
    46021 gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt
    46081 gctcgcgggc atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca
    46141 ccagtgcctc gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg
    46201 aaacaccaag tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg
    46261 caaagcgttt tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat
    46321 cgccagtgtc ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt
    46381 ccagtacacc tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt
    46441 gcaggcgtcc gccctcttcg ataccaggga ccgacaacag gcgtgcatcc attcctggta
    46501 gaatacgccc taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg
    46561 cgcattcggt cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct
    46621 gtttggtact ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt
    46681 catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg
    46741 tgcaactgcg gtcatacacc aagtccggca caatgcggta atgcagcggg cttggataaa
    46801 ggaacacttc tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt
    46861 gaaacagcgg taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa
    46921 tctgctcgac gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt
    46981 cagaaccgga ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca
    47041 ggcgcggcat cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt
    47101 ttaaatcttc cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt
    47161 tgcctttatc gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag
    47221 cactggtcag cccttttacc ccggcagtgt agttcatcat tgcgggaatg cggcgacgcg
    47281 cgatggcccc aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc
    47341 gttcgccttc aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca
    47401 atttgcgata ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga
    47461 agcggtacat tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac
    47521 gcgcttccat cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac
    47581 gcggcgcatc cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga
    47641 acaagcgacg tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa
    47701 tacgcacagg aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga
    47761 ttttcatcag cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc
    47821 cctgttccac cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa
    47881 caaagtcgat aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa
    47941 cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga
    48001 cgtgattagg cgtaattagt acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc
    48061 gaacgcgata caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct
    48121 ttgctctgat tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga
    48181 cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg
    48241 tacgaagagc gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct
    48301 gcacgacgac tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg
    48361 tgtaaattgt cggttactgt tacagattga tgaccggcaa aaaaaaacct gcgcatctgc
    48421 gcaggctggt gtaattcatg tgctcaacac gaagttgact tcacctatca atacctctgg
    48481 gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcgtttc
    48541 gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc
    48601 acattcccac gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc gtaacactga
    48661 aacaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg atgtagcccg
    48721 actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac ccaaagccag
    48781 cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc acccgaacgc
    48841 caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg ttggtgatgt
    48901 ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt atcacaccgg
    48961 taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc aggctattga
    49021 gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga tcccagatgc
    49081 cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc
    49141 tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc tggcaacgcg
    49201 tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc
    49261 tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattccggc
    49321 caatgatcgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac
    49381 cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg attcaatggc
    49441 ggcgggcgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg agatttcgtt
    49501 aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg
    49561 ttacccaatc gtaacgatgg cgacccaggc tgccgaactg gctttggcac tggcggataa
    49621 tcgccctctc ccggaaatta ctaatgtatt tagtccgacg ctggtacgtc gtcattcagt
    49681 gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc agttaaagca
    49741 attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat
    49801 tatcaaacag aacttctggt aacagcggac ggctattgta gttggatgac attgatgcgc
    49861 catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc ggcaaggcgc
    49921 gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca cataacggcc
    49981 cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca gctgccaggg
    50041 cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccggcat
    50101 caaccagcac aaagtggcgg ctacccattt gtttgacgct ccgcacctga gtaattaata
    50161 cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca gggtggccca
    50221 aatggcgagc gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa
    50281 ccgcctcttc accctgttga taaggaatag aaagcccacc gcccgcagaa atagcctgta
    50341 aatcctgacc gaattcgata acctgacgca ccatagcacc acacacctgc tccagatggg
    50401 cataatcaac gccagaacca atgtgcatgt gaatgccgac tagctgcaga tgatgacgtt
    50461 gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc
    50521 caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg cgcagccata
    50581 cccgatgccc tggcgaaacc tggcccagtt ggtcgagcgt atcaacagaa cccgcattca
    50641 ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa
    50701 aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca cgctctattt
    50761 cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc aaaatatgaa
    50821 tattggaaca ggctttctgt gcaaagcgca ccacttcaaa ctgtttcagc gctgcaatct
    50881 gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca gcgggcaaac
    50941 gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt ggcataacaa
    51001 actccagatg agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga
    51061 tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg
    51121 ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga
    51181 ctgaggcagc acacctgcta cacacctcac agccaaccgt cagccgcgaa ctggcgcgct
    51241 ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tggacgatta catcctaccg
    51301 tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg
    51361 tcagcgccgc agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc
    51421 cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg
    51481 atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc
    51541 agcgtcatga tttaggactc actgagacac tccatacgcc tacgggaaca gaacgtaccg
    51601 aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa
    51661 aggtattaac gccggatgat tttcagggtg agaattacat cagcctttcc cgtactgaca
    51721 gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga cgcatgatcg
    51781 tagaaaccca cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggtatttcgg
    51841 tggttaaccc gctcaccgca ctagattatg cggcaagcgg tttagtggtg cggcggttca
    51901 gtattgcggt tccgttcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc
    51961 tggttcaggc gtttagtggg catttacaag cggggttacc gaaactggtc acttctcttg
    52021 acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc
    52081 cgcggtatca aacacaggca aaacactgcg ctcttctggc accagtaaac caatttctgt
    52141 gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata
    52201 ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt
    52261 aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt gttccgtcag
    52321 ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg ccacacgagt
    52381 cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga aaggcagagt
    52441 gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata acacaatacc
    52501 ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc cggttttatc
    52561 ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcat
    52621 ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta
    52681 aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc
    52741 ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag
    52801 gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat
    52861 cagacgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat atgttccagc
    52921 gtgacatttt tggtttccgg aatgagccag aaagtaatac ccacaaacgc aatgttcagc
    52981 gcagtgtaga gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggcc
    53041 gcgccgataa tcatattcga cacccagttt gtcgtcgtcg aacaggtaat accgaaatcg
    53101 cggcatttca gcggctgaat ttcagagcac aggatccaca ccactggcgc ggcgctcatc
    53161 gcataaccgg caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa
    53221 gccgtaccgt tatcaaactg catcaggcaa tagcccagca ccagagtgcc taacgccatc
    53281 acgctgaaac caattttcag agccggttta cgacctgctt tatctaccgt aaagaccgca
    53341 atgaaggtcg cgaacataaa ggtcagtccc acgaccagag tcgcaatcat ctgttgttct
    53401 gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt agtacatgat gatgttcata
    53461 ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa acacagcacg acggacgtta
    53521 cggttgatct taaacagtgc ccaaccgccc tgttttaact tcaggctttc acgaatttcg
    53581 ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc
    53641 tcaatatgac gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga
    53701 ataatcagca gaactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta
    53761 taactgaacg ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac catcaactgg
    53821 tacatactga tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atacagagga
    53881 gcggtgtaag acgcgatccc gacagcaatg cccagcacca cacgagcggc gattaacatc
    53941 tctacgctgg tcgcaaaagc ggaccctata gaaccgagta caaacaggat ggcccccgcc
    54001 atcaggctgt atttacgccc caggcggaac gacagccaac cattaaacag cgcaccaatt
    54061 gctgcgccga gcatcatgct actaaccacc cattcctgca aacgactggt cagcacaaag
    54121 tgatcggtaa tgaacggcaa cgctccggcg attacgccga tatcaagacc aaataacaat
    54181 cctgcgaccg cagcagctac cgaaacaaac atattcatac gccgcgtatc acgcaaagaa
    54241 cgtggcgtta aagcagattc cgtattgata gtaaccattt tttcctgcca gcagagagta
    54301 agacattgtg aaaaaatacg tgaacaattc acacaggtgt caggtcggaa acagcataaa
    54361 tatggattaa attgctgcga catgccgtta tgtgatggat attccaattt taaaattaag
    54421 ttgaattatt gagattatta ttaacctcct aattttaaag cagataaaat tcataaagtt
    54481 cattaattga taattgatat ggattatttc ataaccatga tatggattat gatgatctag
    54541 agatataaaa aaccctgcca tgcggcaggg tcataaaagt aagaggaatg aattaacgcg
    54601 ccagccaacc gccatccaca gcaatggtat aaccatttac ataatctgaa gcgctggagg
    54661 caagaaacac taccggcccc atcaggtcac tcggcagtcc ccaacggcca gctggaatgc
    54721 gatcgagaat ttccgcgcta cgttgttcat ctgcccgcag ttgttgggta ttgttggtcg
    54781 ccatgtaacc cggagctatc gcattaacat taatgttgtg tttagcccat tcgtttgcca
    54841 tcaatcgcgt cacacccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc
    54901 cgccctggaa ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga
    54961 taaagtgttt cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga
    55021 catcgtccca gtctttttcg ctgaactcga gagcgtcttc gcggcgaatc aatccggcgt
    55081 tattcaccag gatatcaata tgaccaaatt cagctaccgc gcgatccagc agtccaggaa
    55141 tgccatcaat ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga
    55201 cctgctcgat ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcccgctt
    55261 gcgccagccc caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa
    55321 ctttaccttg gagagaaaat gcacttaaaa tcataacaac accttattcg tttatgccca
    55381 caactagcgc aaatctttaa cgcccacatg gtccatatca ttaaagacct ggttttcacc
    55441 gaccatgccc cagataaagg tataagcttt ggttccgacg ccggaatgga tcgaccagct
    55501 cggggagatc accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggctgccc
    55561 catcatgtgg aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg
    55621 gcgctcgtgg gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag
    55681 ccccatactc aattgacagg tttccagtac atccgggaca aaatatttgt taatcgtgcg
    55741 acggttactg gtgaggttat cgcctaacgt gactggagat acttcgtccg gtgtgacttt
    55801 tttggtggga tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg ccggagtggc
    55861 ggtatcaata ctggcaaaga caacttcttt tgcaccttta ccaacataca gggcgtcgcg
    55921 gtgaccgatt tcatagcatt gaccatcgac agtaatcgta ccggcaccgc caatattgat
    55981 aacacctaac tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc
    56041 gccaacggaa accgttttag ttatcggcat aatgccgcca acaataattc ggtcaatgtg
    56101 gctgtaaacc atggtgtact catcggcgac aaataccttt tcaaccaaaa attcattgcg
    56161 cagcccttgg gtatccagcg tttttgcgtg cgcaccgtgg atgctctgtc ttacgtccac
    56221 atcaacctcc gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa cactgtttcg
    56281 ttttctctat aaagggatga tatgcccatg ggctagggtt ttcaattaaa aataaaacaa
    56341 tgttttattt ttaatttaag gattaaaaaa agtgcagtgt cgatcacgaa tatgtcatga
    56401 gaatggaaaa atgagggagc ccagaaaatg tatgtcgact tgccagccat cattattcaa
    56461 cagattaatg acggcagata tttatcggta aggaggaaat gcagagctac tcgtcacgtt
    56521 caatggtcaa tgccacgccc tggcccccgc cgatacaaag cgttgccagc cctttgcggg
    56581 cattacgttt caccatttca tgaaccagag aaaccaggat tcggcaaccg gaagcgccta
    56641 tcgggtgacc gagtgcgatc gcgccaccat tgacattgac ccgacgctca tcccactcaa
    56701 gcatcttgcc aaccgaaagc gcctgtgcag caaacgcttc attagcctcg ataagatcga
    56761 catcagccaa ctgccagcct acacgctcca ggcaacggcg ggtcgcatac accggcgcaa
    56821 ttcccatcaa tgccggatct acaccaacgc tggcaaatgc gcggatgcgg gccagcacgg
    56881 gtaaattcaa cgctcgtgct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt
    56941 ttatggatga tgcattcccc gcggtcacag aaccgagact atcaaatgaa ggatttaaac
    57001 gggctaaacc ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg
    57061 tctgaccgtt actttggatc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa
    57121 tcgccgctcg cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga
    57181 tgccatattc gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat
    57241 cccacaaccc atcatgcacc agactgtcaa ccaactggct attgccaagc tgttcaccgg
    57301 tgcggctatc agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca cctgcgatga
    57361 caatatcagc ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagaccgg
    57421 aaccgcaaac gtcattaatc gtgattgcag aaacgctgtt aggaagacca cctttaatag
    57481 ccgattgcct tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt
    57541 catccaccgc atatgcagga acgccggtac gttctattaa cgctttcacg accagactac
    57601 caagttccac ggcggaatga cccgctaacg caccacgaaa gcagccgata ggtgtccgta
    57661 acgccccgac aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt
    57721 aaatgattgt taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg
    57781 aaatatgcct gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc
    57841 caccaataga tgggtagata gggcgttaat ctcccatact taacttggtt tatggtaaat
    57901 tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct
    57961 aaagaagaaa ctctgtgaag tttcgcgctc tatggcacag ccgttgatgc aggcacgcta
    58021 ttccttccta ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc
    58081 gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct ctcatcaaaa
    58141 acatcagcag gtgaagggat aacgggcgcg gtaacacact actatggcaa gaagattggt
    58201 aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg
    58261 gcaattacca actcacttac ggaacagctg gcaaagcata tggttattga tcttcgcatc
    58321 cgtatgttgg tgagtctggg tgttgtatta attctgaatc tcatttttct gatgggacgt
    58381 catgccacta ttcgggtaat gggatttttg gtattcccat tgattgccta tttcttattt
    58441 ctttccattt accttgtcgg tagttggcaa cctgatctat taacaaccca ggtagagttc
    58501 aatcagaata cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc
    58561 catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct
    58621 atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg
    58681 ttctttgtct ttagctgcct gctttctatt ccatcttcgt atattgaagc ggctaaagaa
    58741 gaaggggtca ccattttatc ggcgctttct atgctgccga acgccccagc atggttgtca
    58801 atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg tacgtacttt
    58861 ggcgttattg aaggtgccac agaggtcgtc aaaacaacac tacagcaggt tggtgtaaag
    58921 aaaagtcgtg catttaacca cgcactatca attatgttgg tatcgctgat taccttcatt
    58981 gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt
    59041 gccatgatac ttttcatcat gcctacgctg tcaacgtatc tcatcccggc gcttaaaccc
    59101 tggcgttcca tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg
    59161 ttctttagct aatatccgtt gtggatggtt caaaattgct acaattttga accattccat
    59221 ttttcataat attcatttgt tattattccg ccctatcact tctctcgtca tatcctagaa
    59281 ttatcagtta gttaaacaca caaagagatc tgaacatctt aaataagact atttaagatg
    59341 cataacttag attcgcaaga tatcttctgg tcattcaaaa acaatttgcg atatagatat
    59401 tctcatacca tgagatagtt atctggacca cgttcgacca tgcaagatta aatcagtgaa
    59461 acataatcat atttaataac gagaaatgca tttttaaata tcttaatcag aattatagca
    59521 aatacagatt aatcgcaatt tttcccagca aaattacaaa atcaaggtgt attgacatca
    59581 tcaatcacca atgaaattat gcaattatat acggataggg aggttcttaa catggggctt
    59641 tgtagtcgtt ataaaagtct tacatgcaat agttgctcaa tgcattgcca aataatgcca
    59701 gaagagtcac cgcgtttgca gtactgcgca aactcgtgtt
//
LOCUS       DAJTRN010000013        58031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRN010000013 DAJTRN010000000
VERSION     DAJTRN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(24..278)
                     /locus_tag="OO328_000824"
     CDS             complement(24..278)
                     /locus_tag="OO328_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5929490.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO328_000825"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO328_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5929491.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO328_000826"
     CDS             1228..2694
                     /locus_tag="OO328_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5929492.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO328_000827"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO328_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5929493.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO328_000828"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO328_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5929494.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO328_000829"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO328_000829"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5929495.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO328_000830"
     CDS             5709..7265
                     /locus_tag="OO328_000830"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5929496.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO328_000831"
     CDS             7347..9161
                     /locus_tag="OO328_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5929497.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO328_000832"
     CDS             9162..10256
                     /locus_tag="OO328_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5929498.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO328_000833"
     CDS             10256..11281
                     /locus_tag="OO328_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5929499.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO328_000834"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO328_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5929500.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO328_000835"
     CDS             complement(12876..13220)
                     /locus_tag="OO328_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929501.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO328_000836"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO328_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5929502.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO328_000837"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO328_000837"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5929503.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO328_000838"
     CDS             15616..17376
                     /locus_tag="OO328_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5929504.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO328_000839"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO328_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5929505.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO328_000840"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO328_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5929506.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO328_000841"
     CDS             19113..19340
                     /locus_tag="OO328_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5929507.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO328_000842"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO328_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5929508.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO328_000843"
     tRNA            21195..21271
                     /locus_tag="OO328_000843"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO328_000844"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO328_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5929509.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO328_000845"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO328_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5929510.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO328_000846"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO328_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5929511.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO328_000847"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO328_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5929512.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO328_000848"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO328_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5929513.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO328_000849"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO328_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5929514.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO328_000850"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO328_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5929515.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO328_000851"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO328_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5929516.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO328_000852"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO328_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5929517.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO328_000853"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO328_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5929518.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO328_000854"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO328_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5929519.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO328_000855"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO328_000855"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5929520.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO328_000856"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO328_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5929521.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO328_000857"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO328_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5929522.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO328_000858"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO328_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5929523.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO328_000859"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO328_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5929524.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO328_000860"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO328_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5929525.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO328_000861"
     CDS             37391..37555
                     /locus_tag="OO328_000861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929526.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO328_000862"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO328_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5929527.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO328_000863"
     CDS             complement(38300..39331)
                     /locus_tag="OO328_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5929528.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO328_000864"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO328_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO328_000865"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO328_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5929529.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO328_000866"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO328_000866"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5929530.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO328_000867"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO328_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5929531.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO328_000868"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO328_000868"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5929532.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO328_000869"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO328_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5929533.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO328_000870"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO328_000870"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5929534.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO328_000871"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO328_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5929535.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO328_000872"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO328_000872"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5929536.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO328_000873"
     CDS             complement(53338..54114)
                     /locus_tag="OO328_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5929537.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO328_000874"
     CDS             complement(54119..55768)
                     /locus_tag="OO328_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5929538.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO328_000875"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO328_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56332..56490)
                     /locus_tag="OO328_000876"
     CDS             complement(56332..56490)
                     /locus_tag="OO328_000876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929539.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56487..>58031)
                     /locus_tag="OO328_000877"
     CDS             complement(56487..>58031)
                     /locus_tag="OO328_000877"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5929540.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14198 a        15655 c        14256 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taaccctcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtagc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga cgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta a
//
LOCUS       DAJTRN010000014        54881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRN010000014 DAJTRN010000000
VERSION     DAJTRN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO328_000878"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO328_000878"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5929541.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO328_000879"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO328_000879"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5929542.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO328_000880"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO328_000880"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5929543.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO328_000881"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO328_000881"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5929544.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO328_000882"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO328_000882"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5929545.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO328_000883"
     CDS             7826..9559
                     /locus_tag="OO328_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5929546.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO328_000884"
     CDS             9708..10559
                     /locus_tag="OO328_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5929547.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO328_000885"
     CDS             complement(10546..10887)
                     /locus_tag="OO328_000885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5929548.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO328_000886"
     CDS             complement(10889..11767)
                     /locus_tag="OO328_000886"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5929549.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO328_000887"
     CDS             complement(11733..14030)
                     /locus_tag="OO328_000887"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5929550.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO328_000888"
     CDS             complement(14081..14401)
                     /locus_tag="OO328_000888"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5929551.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO328_000889"
     CDS             complement(14416..15495)
                     /locus_tag="OO328_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5929552.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO328_000890"
     CDS             15804..16907
                     /locus_tag="OO328_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5929553.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO328_000891"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO328_000891"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5929554.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO328_000892"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO328_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO328_000893"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO328_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO328_000894"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO328_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO328_000895"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO328_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5929555.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO328_000896"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO328_000896"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5929556.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO328_000897"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO328_000897"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5929557.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO328_000898"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO328_000898"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5929558.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO328_000899"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO328_000899"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5929559.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO328_000900"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO328_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5929560.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO328_000901"
     CDS             30120..30728
                     /locus_tag="OO328_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5929561.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO328_000902"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO328_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5929562.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO328_000903"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO328_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5929563.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO328_000904"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO328_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5929564.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO328_000905"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO328_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5929565.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO328_000906"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO328_000906"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5929566.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO328_000907"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO328_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5929567.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO328_000908"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO328_000908"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5929568.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO328_000909"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO328_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5929569.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO328_000910"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO328_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5929570.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO328_000911"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO328_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO328_000912"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO328_000912"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5929571.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO328_000913"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO328_000913"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5929572.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO328_000914"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO328_000914"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5929573.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO328_000915"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO328_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5929574.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO328_000916"
     CDS             complement(44843..45142)
                     /locus_tag="OO328_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5929575.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO328_000917"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO328_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO328_000918"
     CDS             45895..46494
                     /locus_tag="OO328_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5929576.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO328_000919"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO328_000919"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5929577.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO328_000920"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO328_000920"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5929578.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO328_000921"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO328_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5929579.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO328_000922"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO328_000922"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5929580.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO328_000923"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO328_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5929581.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO328_000924"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO328_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5929582.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO328_000925"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO328_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5929583.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO328_000926"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO328_000926"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5929584.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..>54881)
                     /locus_tag="OO328_000927"
     CDS             complement(54612..>54881)
                     /locus_tag="OO328_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5929585.1"
                     /translation="MQNTGKVGWLYSVIAPGKVSADAPLELVSRVSDVTVQEAAAIAW
                     HMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFSRRLWGK"
BASE COUNT        13364 a        14864 c        14046 g        12607 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcggggtca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca t
//
LOCUS       DAJTRN010000015        53597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRN010000015 DAJTRN010000000
VERSION     DAJTRN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(2..298)
                     /locus_tag="OO328_000928"
     CDS             complement(2..298)
                     /locus_tag="OO328_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5929586.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="OO328_000929"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="OO328_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5929587.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO328_000930"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO328_000930"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5929588.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="OO328_000931"
     CDS             complement(1784..2323)
                     /locus_tag="OO328_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5929589.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="OO328_000932"
     CDS             complement(2353..3096)
                     /locus_tag="OO328_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5929590.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="OO328_000933"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="OO328_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="OO328_000934"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="OO328_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5929591.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="OO328_000935"
     CDS             complement(4151..4657)
                     /locus_tag="OO328_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5929592.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="OO328_000936"
     CDS             complement(4703..5203)
                     /locus_tag="OO328_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5929593.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="OO328_000937"
     CDS             complement(5289..5468)
                     /locus_tag="OO328_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5929594.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO328_000938"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO328_000938"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5929595.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO328_000939"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO328_000939"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5929596.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO328_000940"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO328_000940"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5929597.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO328_000941"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO328_000941"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5929598.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO328_000942"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO328_000942"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5929599.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO328_000943"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO328_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5929600.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="yciV"
                     /locus_tag="OO328_000944"
     CDS             12652..13533
                     /gene="yciV"
                     /locus_tag="OO328_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5929601.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="OO328_000945"
     CDS             13530..14150
                     /locus_tag="OO328_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5929602.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="OO328_000946"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="OO328_000946"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5929603.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO328_000947"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO328_000947"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5929604.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="OO328_000948"
     CDS             complement(15750..16508)
                     /locus_tag="OO328_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5929605.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="OO328_000949"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="OO328_000949"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5929606.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="OO328_000950"
     CDS             complement(17813..18064)
                     /locus_tag="OO328_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5929607.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="OO328_000951"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="OO328_000951"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5929608.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21251..22225
                     /gene="cysB"
                     /locus_tag="OO328_000952"
     CDS             21251..22225
                     /gene="cysB"
                     /locus_tag="OO328_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5929609.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22520..22684
                     /locus_tag="OO328_000953"
     CDS             22520..22684
                     /locus_tag="OO328_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5929610.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22687..22854
                     /locus_tag="OO328_000954"
     CDS             22687..22854
                     /locus_tag="OO328_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929611.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22968..23063
                     /locus_tag="OO328_000955"
     CDS             22968..23063
                     /locus_tag="OO328_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929612.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23227..25902
                     /gene="acnA"
                     /locus_tag="OO328_000956"
     CDS             23227..25902
                     /gene="acnA"
                     /locus_tag="OO328_000956"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5929613.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO328_000957"
     CDS             complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO328_000957"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5929614.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26726..27490
                     /gene="pgpB"
                     /locus_tag="OO328_000958"
     CDS             26726..27490
                     /gene="pgpB"
                     /locus_tag="OO328_000958"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5929615.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27639..27947
                     /gene="lapA"
                     /locus_tag="OO328_000959"
     CDS             27639..27947
                     /gene="lapA"
                     /locus_tag="OO328_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5929616.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27954..29123
                     /gene="lapB"
                     /locus_tag="OO328_000960"
     CDS             27954..29123
                     /gene="lapB"
                     /locus_tag="OO328_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5929617.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            29316..30053
                     /gene="pyrF"
                     /locus_tag="OO328_000961"
     CDS             29316..30053
                     /gene="pyrF"
                     /locus_tag="OO328_000961"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5929618.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            30053..30379
                     /gene="yciH"
                     /locus_tag="OO328_000962"
     CDS             30053..30379
                     /gene="yciH"
                     /locus_tag="OO328_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5929619.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO328_000963"
     CDS             complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO328_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5929620.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30992..31741)
                     /locus_tag="OO328_000964"
     CDS             complement(30992..31741)
                     /locus_tag="OO328_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5929621.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(31831..32004)
                     /locus_tag="OO328_000965"
     CDS             complement(31831..32004)
                     /locus_tag="OO328_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929622.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO328_000966"
                     /pseudo
     CDS             complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO328_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            34180..34283
                     /locus_tag="OO328_000967"
                     /pseudo
     CDS             34180..34283
                     /locus_tag="OO328_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO328_000968"
     CDS             complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO328_000968"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5929623.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(36363..37490)
                     /locus_tag="OO328_000969"
     CDS             complement(36363..37490)
                     /locus_tag="OO328_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5929624.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO328_000970"
     CDS             complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO328_000970"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5929625.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(38790..39143)
                     /locus_tag="OO328_000971"
     CDS             complement(38790..39143)
                     /locus_tag="OO328_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5929626.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO328_000972"
     CDS             complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO328_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5929627.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO328_000973"
     CDS             complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO328_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5929628.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO328_000974"
     CDS             complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO328_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5929629.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(41888..42853)
                     /gene="sapB"
                     /locus_tag="OO328_000975"
     CDS             complement(41888..42853)
                     /gene="sapB"
                     /locus_tag="OO328_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5929630.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(42850..44493)
                     /gene="sapA"
                     /locus_tag="OO328_000976"
     CDS             complement(42850..44493)
                     /gene="sapA"
                     /locus_tag="OO328_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5929631.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(44806..45051)
                     /locus_tag="OO328_000977"
     CDS             complement(44806..45051)
                     /locus_tag="OO328_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5929632.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(45185..46570)
                     /gene="puuP"
                     /locus_tag="OO328_000978"
     CDS             complement(45185..46570)
                     /gene="puuP"
                     /locus_tag="OO328_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5929633.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(46560..46724)
                     /locus_tag="OO328_000979"
     CDS             complement(46560..46724)
                     /locus_tag="OO328_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929634.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(46873..48291)
                     /gene="puuA"
                     /locus_tag="OO328_000980"
     CDS             complement(46873..48291)
                     /gene="puuA"
                     /locus_tag="OO328_000980"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5929635.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            48503..49267
                     /gene="puuD"
                     /locus_tag="OO328_000981"
     CDS             48503..49267
                     /gene="puuD"
                     /locus_tag="OO328_000981"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5929636.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            49294..49851
                     /gene="puuR"
                     /locus_tag="OO328_000982"
     CDS             49294..49851
                     /gene="puuR"
                     /locus_tag="OO328_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5929637.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            50001..51488
                     /gene="puuC"
                     /locus_tag="OO328_000983"
     CDS             50001..51488
                     /gene="puuC"
                     /locus_tag="OO328_000983"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5929638.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            51490..52770
                     /gene="puuB"
                     /locus_tag="OO328_000984"
     CDS             51490..52770
                     /gene="puuB"
                     /locus_tag="OO328_000984"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5929639.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            52808..>53597
                     /locus_tag="OO328_000985"
     CDS             52808..>53597
                     /locus_tag="OO328_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5929640.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLM"
BASE COUNT        13435 a        13454 c        13869 g        12839 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca
    21121 cctatacact aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa
    21181 atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg
    21241 atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct
    21301 gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt
    21361 cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac
    21421 gcaggtaacg ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt
    21481 cgatgccata aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat
    21541 cgccaccacg catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga
    21601 gcgttatccc cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc
    21661 cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga
    21721 tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc
    21781 gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata
    21841 taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac
    21901 gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg
    21961 gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt
    22021 gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag
    22081 tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg
    22141 tgatgtcgtt gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa
    22201 agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg
    22261 aaggtgccgg aatatattct cttttgttac ttatttctat accgtgcaca atatttttat
    22321 ccctcatatt tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaat
    22381 tccagttttc aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg
    22441 taaattcgct ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt
    22501 acaccaatca ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc
    22561 ttctggaaat caggaacccc gccgtgatcc tgaattaaaa cgtaaagcct ggctggcggt
    22621 ttttcttggt tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg
    22681 ataactatgg tcggtcagga gcaactggag tcgtcaccat tatgccagca tagtgacaat
    22741 gaaccagaaa cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg
    22801 cagcagcaag cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca
    22861 tcattcttat tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca
    22921 gcttgagtta tctcaacaca aaataataac cgttaagggt gtagcctatg atcaacacaa
    22981 atatgaaata ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac
    23041 tcctctggat cattactcac tgatccttga ccccgctgcg gcggggttgt catttgcttt
    23101 gccacaaggt ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt
    23161 gtgttatctt taatattcac cctgaagaga atcagggctt cgcaaccctg tcattaagga
    23221 ggagctatgt cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa
    23281 acttaccact actacagcct gccgcttgct gctaaatcac tgggcgatat cacccgtcta
    23341 cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg taactcagtt
    23401 accgaagagg atatccacgc actggcggga tggctgaaaa atgcccatgc tgaccgtgaa
    23461 attgcctacc gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt
    23521 gatctggcgg caatgcgcga agcggttaaa cgcctcggtg gcgatactgc aaaggttaac
    23581 ccgctctcac cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat
    23641 gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg
    23701 ttcctgaaat ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc aggcacaggc
    23761 atttgccatc aggttaacct cgaatatctc ggcaaagcag tgtggagcga attgcaggac
    23821 ggtgaatgga ttgcttatcc ggatacactc gttggtactg actcgcacac cactatgatc
    23881 aacggccttg gcgtgctggg gtggggcgtg ggtgggatcg aagcagaagc cgccatgtta
    23941 ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac cggaaaatta
    24001 cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct gcgcaaacat
    24061 ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc tggattcact accgttggcg
    24121 gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca
    24181 atcgatgcag taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggtcgag
    24241 ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt
    24301 tttaccagta tgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa
    24361 cgcccacagg atcgcgttgc actgcccgat gtaccaaaag catttgccgc cagtaacgaa
    24421 ctgaaagtga atgccacgca taaagatcgc ctgccagtcg attatgttat gaacggacat
    24481 cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc
    24541 tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt aactcttggc
    24601 ctcaagcggc aaccgtgggt caaagcgtcg ttggcaccgg gttcgaaagt cgtttctgat
    24661 tatctggcaa aagcgaaact gacaccgtat ctcgacgaac tggggtttaa ccttgtggga
    24721 tacggttgta ccacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca
    24781 atcaaaaaag gcgatttaac cgtcggtgcg gtgctgtccg gcaaccgtaa ctttgaaggc
    24841 cgtatccatc cgctggttaa aactaactgg ctggcctcgc caccgctggt ggttgcctat
    24901 gcgctggcgg gaaatatgaa tatcaacctg gcttctgagc ctatcggcca tgatcgcaaa
    24961 ggcgagccgg tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta
    25021 gaacaagtct ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca
    25081 gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc
    25141 tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat
    25201 attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct
    25261 ccggcgggca gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt
    25321 gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc
    25381 ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg
    25441 acgcggcatt tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag
    25501 caggagcaaa cgccgctggc ggtgattgcc gggaaagagt atggatcagg ctcaagccgt
    25561 gactgggcgg caaaaggacc tcgtctgctt ggaattcgtg tggtgattgc cgaatcgttt
    25621 gaacgaattc accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa
    25681 ggcgtgacgc gtaaaacgtt agggctaacc ggggaagaga agattgatat tggcgatctg
    25741 caaaatctac aacctggcgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag
    25801 gaagtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac
    25861 gacggcattt tgcattatgt cattcgtaat atgttgaagt aacaagtatt tgcttgccgg
    25921 ttattttgct tccggcaagc aaatgaatta cacgatgcaa gagggttatt tgttcagcaa
    25981 atggcccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac
    26041 aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt
    26101 cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca
    26161 aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg tggttagcct ctacggtatc
    26221 gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc
    26281 ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc
    26341 ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc
    26401 ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga ctagcgcaac
    26461 atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt
    26521 tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt
    26581 ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg
    26641 ttcacggaat ggcacaaatc tgtcaatttt tcttggaact ggcattttca gttatgattg
    26701 tgggacttat caaaaaggag aggccatgcg ttcgattgcc cgacgtaccg cagtgggagc
    26761 tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg
    26821 agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca cccagccctg
    26881 gggcgtcatt acacatttga ttttgttcgg ctggtttctc tggtgtctgc gttttcgcat
    26941 taaggctgcc tttgtattat ttgccattct ggcggcggca atccttgttg gacaaggcgt
    27001 taaatcctgg atcaaagaca aagtacagga accacgacct tttgttatct ggttggaaaa
    27061 aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac gcggaaatct
    27121 agtaaaagaa cagttggctg aagagaaaaa tattccacaa tatttgcgtt cacactggca
    27181 gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact
    27241 gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgttggt
    27301 ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc cacgcgatct
    27361 ggtggtagct acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc ttgcgcaacg
    27421 aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaattgcac aacgagaaca
    27481 agaaagttaa cgctggttga ttttccgaat ttaggcctta aatcatcaac aatgcgcgtg
    27541 gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac
    27601 cataatttat tcgctctaac cacataacgg gaagtaatgt gaaatattta ctcattttct
    27661 tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg
    27721 tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat
    27781 tgtttgctgc ggggtttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc
    27841 gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg
    27901 cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg
    27961 agttgttgtt tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa
    28021 gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg
    28081 ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta
    28141 aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg
    28201 gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcgctgacct
    28261 atgaacagcg tctgttggcg attcaacaac tggggcgtga ttacatggcc gccgggttat
    28321 acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg
    28381 cgctgcaaca gttgctacaa atctaccagg ctaccagtga gtggcagaaa gcaattgatg
    28441 ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct
    28501 actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc
    28561 taaaaaaagg ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg
    28621 tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtcatatccc
    28681 aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat cagcagttgg
    28741 gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac accggtgccg
    28801 atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gctgcacagg
    28861 tctatattac acgccagctt cagcgtcatc cgaccatgcg tgtattccat aagctaatgg
    28921 attaccactt aaatgaagcg gaagaagggc gcgccaaaga gagtctgatg gtgctgcgtg
    28981 acatggttgg cgagaaggtg cgtagtaagc ctcgttatag ctgccagaaa tgtggtttta
    29041 ccgcatacac cctctactgg cattgtccgt cttgtcgtgc ctggtcaact attaaaccga
    29101 ttcgcggtct tgatggcctg taatttttaa aaaaatccga ctttagttac aacatactag
    29161 ttattgatgt cccattgtgc tccggcaacg acggagcaga aaaagcctgc taggggaaaa
    29221 atcgcaactg ttaatttttt attttcacgg gtagaatgct cgccgtttac ctgtttcgcg
    29281 ccactcccgg tgcccatcat caagaaggtc tggtcatgac gttaactgct tcatcttctt
    29341 cccgcgccgt tacgaattct cctgtggttg ttgcccttga ttatcataat tgtgatgacg
    29401 cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc ggtaaagaga
    29461 tgtttacatt gtttgggcca cagtttgtgc gcgaacttca acagcgtggt tttgatatct
    29521 ttcttgacct gaaattccac gatatcccca acactgcagc gcacgctgtc gctgctgcag
    29581 ctgacttagg cgtgtggatg gtgaatgttc atgcctccgg tggggcgcgt atgatgaccg
    29641 cagcgcgtga ggcactggtt ccgtttggca aagatgcacc gcttttgatt gctgtgacag
    29701 tgttgaccag catggaagcc agcgacctgg ccgatcttgg cgtgacactg tcacctgcag
    29761 attatgcaga acgtctggca gcactgacgc aaaaatgtga ccttgatggt gtggtgtgtt
    29821 ctgctcagga agctgtgcgc tttaaacagg tattcggtca ggagttcaaa ctggttacac
    29881 cgggcattcg tccgcagggg agtgaagctg gtgaccagcg ccgcattatg acgccagaac
    29941 aggcgttggc ggctggtgtt gattatatgg tgattggtcg cccggtaacg caatcggtag
    30001 atccagcgca gacactgaaa gcgatcaacg cctctttaca gcggagtgca tgatgagtga
    30061 ttccaacagc cgtctggtct actcaacgga aaccgggcgt attgatgagc ccaaagcggc
    30121 cccagtacgc cctaaaggcg atggagcggt gcgtattcag cgtcagacca gtggacgtaa
    30181 aggtaagggc gtttgcctga ttaccggtat cgatctcgat gatgccgaac tgacaaaact
    30241 tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca gttaaagatg gagttattga
    30301 aatccagggc gataagcgtg atttattaaa atcactactt gaagcgaaag ggatgaaggt
    30361 aaaactcgca ggcggttaac ataaaaagcc acggatatat ccgtggcttt cgaatatttt
    30421 actgtgtgta ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag
    30481 tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa caccaccgac
    30541 agctgcacca cctaatgtac ccaacgtact gccatcggtc agtactgcac cgcctaatgc
    30601 ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta gaccagttag aacaggcact
    30661 cagagacatt gccaaagtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa
    30721 cataatactc tctcctgaat ttatgattca cggaagtaag ctctctataa ctataatagc
    30781 taagaataag cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg ttatatcacg
    30841 caggtgataa ttacttcctg ttatatcgct gataataatt ttatatcttg agagtgttaa
    30901 taacaggtaa atagtcttaa ttatcaacca ggaatcatct tagagcggat gatttgccaa
    30961 actgcaaatc atccgtagag aagggaaatg gttaaatgtc aacgacgtga acggtgagtt
    31021 tactgtgctc aagatgcatc agatcgctgg cgcgtatttt cgaatcggta atcacgcgat
    31081 tgaatcgctc aacgggaccg atggagtatg aatgtacagc accaaatttc gagctgtcag
    31141 tcaggactat cgcttcgcac tctttttcca gcacggcatt gaccacatcg gtgcgcatca
    31201 tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc aataccaata aatgctttgc
    31261 tgaaatgtac ctgctggatg cactggcgtg tcaaagggcc aaccatactt tcgctttttt
    31321 tctggtacac gccaccgagc aaaatgactt cacaaggcgc gtctttcagc aaatgcgcga
    31381 tgtagctgct gaccgtgatg atagtgacat ttttcttctg ctcccccaga gtccgggcga
    31441 gcagggcatt gctgctgcca ttttcgataa agatggtttc gcccggttga accagtgacg
    31501 cggcaaactc ggcgagttca cgcttcagcg tatagttgct catcatacgg gtttcgacgt
    31561 catcactatc aagcgaaact gcaaagccat gtgcacggcg gaggtaactc agtttttcga
    31621 gggtgttgag atcctggcga atggtaactt cagaaactcc agtggctttt gccagatcgg
    31681 ttacgctaac ctgaccctgg tcaatgacca tctgtagaat agtttgttgt cgggaattca
    31741 tcgctgtaat tcttataacg ttataatact aaataaaaaa atactgacgc gccgggcatc
    31801 ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc cacggtgtct tcgggatgtc
    31861 tggcgtagat tcagcaacct ggcgcaagag ttctgcttta agtatctcga gattatggat
    31921 cgcagagtgg taatcgcctg ccacaaggat atctaacaca atatcaatac gttcggcaac
    31981 gcgctgggca tcaaattcag acatcaagga catccttata gcaaaagggg gaaaatgaat
    32041 aatgcaaaaa ttgccgctaa aagagaagtg tttaacagca acggctaatt atcatccagg
    32101 aatacgatat ataatgacgg gatatagcgc taagtatata tattcatcta cttatgcgcg
    32161 cttcagatag cgtttatacc agcgttcgaa ggcgacggcg ggcatcggtt tggcaaacaa
    32221 aaatccttgc cgctcattga tcccgttctt ggttaaaaaa gcatcttcct ttgcactctc
    32281 tacaccttcg gcgatcacct gaagattcaa tgcctgggcc acagcgacga tcgcccggac
    32341 cagtgactgc gagacaggtt gtttgtgaat atctcgaaca aaaacctggt caagtttgat
    32401 ggcatcgatc ggaaagcgcg ccagttgcga aagtgaagag tagccggtac caaaatcatc
    32461 cagatgcact tgcgcaccta gttggctaaa ttgttgaata acagacagtg ccagttcatc
    32521 attctcaatc agacaactct ctgtcagttc aacatctata gggcagtatt caaaattgag
    32581 ttcctggaga acctgtttca gggcggtgaa aatggtttga tcggcgagct gacgtgcaga
    32641 aatatttacc gccactcgca ggtttatgcc tttatcccgc cactttgcca cctggcgtac
    32701 gacatcgaga atcacccaac ggcctaaagg cacaattagc cctgactctt cggcgtagga
    32761 aatgaagtcc aacggtggaa tcaacccacg ttcaggtgac tgccaacgta ctagtgcttc
    32821 cagactgcgc acttcgacac gctaggtgat tttcgataac caactgatcg ttttccagtg
    32881 ctttacgcaa gttggtatcc agccagagat attcaaatac ccgctgattc atttctggag
    32941 taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt gtacattgct gtgtcggcgt
    33001 gacgaataat agtcgcgctg tccgaaccat gttcgggaga gagcgaaata cctactgaac
    33061 agctggtata aacttcaatt aaaccaatac gaaaggggag gcgtaagcgg gtcaaaattc
    33121 gtgatgccat tgcttccagc gcgctttgtg aggtattgga tgccagtacc agaaactcat
    33181 cgccacctgg acgcgccaac acctggtcat gttcgagaca gcttaaaata gccaatgaca
    33241 cgtcgcgtaa taactggtca ccaaacaaat gcccataggc gtcgttgacc tttttaaaat
    33301 tatccaaatc aagatacaca accccaactt tattgttatc tgcctgatta atagcgtgat
    33361 cgattaactc ctgcattgcg ttacgattcg gcagtccggt gatactgtcg gtatttgcca
    33421 gaatacgcag tcgctcctga gtgcggcgct cttcggtaat gtcggtaccg gaacagatta
    33481 aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt attgcgaaac agaaacagcc
    33541 gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg cttcgaaaaa
    33601 atacacggtt attgcgcctg gatgccgcag cttcacgacg gctcataaac agtttaaaca
    33661 cgctttaccc aatgacgtcg tgttctttca accctgtgta atcttcacat aaccgattga
    33721 agcgttggat attcccccgg ctgtcgagaa tcactattgc ggaatgtgct tcagaaacta
    33781 cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc aaccgccggt gtgtcatgcc
    33841 atgcagacgc gttgccagcc cattcccgtt tattgatttt tcgtcctacc agatgcacag
    33901 aaagatcgct ttcatcgacg gtcagactca tcatcaggct ggaggtgatg accgtcattt
    33961 ccctgatgcg agcagcctgt tcggcagata actgcaatgt acgatcaggt tctgtggttt
    34021 tggtggtaga aaagcgcaaa gcatcgctgc tttccgtcaa ccgccagtat ggattgtgcg
    34081 atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg catatcctcc
    34141 ggttaacagc ggagtccgcg gttaaataaa aggaacaaca tgacttacat gaaattaacg
    34201 gcggctaaac gcattgtttt tttatatatt tatttgtaat ccagttttgg aaaaacgcca
    34261 gttttcaaac gaaagtcagt taaaaaatct gcctggatat aacgaaggta gagcggggaa
    34321 ataaacggcc catccatgag gaatgggccg tgaaaggaga ttacgcgacc gggcgcgcaa
    34381 taatgctgcg ggtttccatg cggacttcgg caatggtgac gtcaataacg tcagttactt
    34441 tgtaaaccgt ttcaccttta atttgtacag tgccgttttc ctggctgcaa accagttcat
    34501 cgcgcacagc gtgtaagaaa ggtgccggaa taaaggcgat agcgccgtta tcaaccaaac
    34561 gcacacgcat gccgccacgg ctgatatcga caatttccgc cgcgaaacgg gtgccggtcc
    34621 cggctttatc tttcaggaag cgtgcgtata accagtcgcc aacatcacgt tctgccatcc
    34681 ggttgagacg gcgacgttcg gccatttgga cagtgatttc atcctgcgga cgcgtcgcag
    34741 tttcgccttt gataaccgct ttcagcagac ggtggttgat catgtcgcca tatttacgga
    34801 tcggcgaagt ccaggtggcg tatgcttcca gaccgaggcc aaagtgagga ccgggttcag
    34861 agctaatttc agcaaatgac tggaagcgac gaatgcggct gtcgaggaaa ccagttggtt
    34921 gcgcatccag ttcacgacgc agtttgcaga aaccgtccag cgtgagcact tcttcggcat
    34981 cgacatgcag accgtgcgtt ttcagcaacg ctgccagcgc gtcggcattc gccggatcaa
    35041 agcccatatg cacgttatag atgccaaaac cgagcttatc gcgcagtacg cgggccgcac
    35101 aaatgttagc ggcaatcatc gcttcttcga cgatacggtt ggcaatgcga cgaggctcgg
    35161 cgacgatatc cagcacttca cctttttcac cgagaataaa gcggtaatcc gggcgatctt
    35221 taaacaccag tgcgtggtta tgacgccact cgccgcggcg ttggcaaatt tgcgctagca
    35281 aacggacttg ttcggcaatt gcttcacttt caggcttcca gtcaccggta ttttccagcc
    35341 agtcagaaac ctggtcatac accagcttcg ctttggattc gatggtggcg gcaaagaatt
    35401 cgatattatc ttcaatggtg ccatcagcgg agagcgtcat gcggcatgcc agtaccgggc
    35461 ggacttcatt ggcgcgcagt gagcagagat cgtcagaaag ctcgcgaggc agcataggga
    35521 tgttgaagcc aggcagatag ttggtgaatg cgcgaatttt cgcggctttg tccagcttgc
    35581 tgccttcagc aatccacgcg gttggatcgg caatcgccac aatcagctga agtttgtcat
    35641 ccggcagcgc cttagcgaaa agggcgtcat ccatatcttc agtgctggca ctgtcgatgg
    35701 tgacaaaatc cagcgaggtc agatcttcac gaaccagacc ttcatcgagc atttcggtag
    35761 cgacgccgtc tggtgcttct ttttccagat tatggcgcgc aagggtaacc caccacggta
    35821 caaagtggtc gtcaccaaaa gtgatgtatt gtgtcagttc agcatagaaa gaacgatcgc
    35881 ctttcagcgg atgacggcgc atttcggcaa ccgcccagtc gccttcttta aactcgtggt
    35941 tcaggccacg ggctgcgcgg caaggaatgg cgtcttttaa aagtggatga tcaggaacga
    36001 tggtcagacg gtcatttttg ccctgaactt tacccacgaa acgagtcagg aacggttcaa
    36061 ccagttcttc tggctctgcg gattcacgtt ctttttcact gtggatcacc gcgataattc
    36121 ggtcgccatg catgactttt ttcatctgcg gcggcggaat gaaataactt ttttgtgcgt
    36181 cgacttccag gaagccaaag cctttttctg tggcttttac caccccttca gcgcgtggcg
    36241 tctgggaatg cagttgctgt ttaagctgcg ctagcagcgg gttgtcctga aacataattg
    36301 tctattttgg tggccattag agcggctgac agttttacgc gaatctgtct gacgcggcaa
    36361 ggttaatatg tctcacccaa cgcgattttt aagcgattta tccagccaca cagcccgctc
    36421 cataccagca gattaataat ctgcgttgat gatttttcgt gttcgagtaa agggctgaac
    36481 tgggcggcgc taaagcgatc gggtgctcgg gtcagccatt gtatcgcctg aacggtagcg
    36541 cgctcaacag aatgacggtc agcccatcgg ttaatttcat tttcgccctt gcgcagaagg
    36601 gtgctgattt ttttatattc atttgatgaa tccatacagg tggaaaaaca agcaggactg
    36661 ccatttatac gcgcgcataa caacgccgca agctgattat ccggctgtgc attgacccgt
    36721 gtgctgttta acaacttttc gagaagggaa agtgaaatag ggtggagaga gagtgtttcg
    36781 gccagttggc aaagttctgc agttgaactt tttgtgtgct cttcaggtag tttttccacc
    36841 tcatagctgc ttcgccagcg tattgattca tcagcaaaca gtgacgcgtc ggcgtagttt
    36901 tgtatcgcca gcccgggtaa ccagcgtacc ggatgcccga gataagcctg aaatgtcgca
    36961 attgtccgcg cctgaaagcc aataaaacca atgatttggt taatgatcac acagtcccag
    37021 acagtaagac cgacgtcatt cagatgctgg cgcgatcggt tgtcgataat agaaggcgag
    37081 ctagccagtt ggcgggcata ttgcgtgatt tgtgccaacc gatgattact ttccctggag
    37141 gaatcaggtc cggggagcgg cgtaagtcgc gccgaatagt agttacataa ccgctggacg
    37201 ccgcaaacct gagcaaccgt cagggccgtc gacaagcgtt cataagcggt gaaggtttgt
    37261 gaacgggtca cggtaagctg gtcagggaac aactccactg caagaacgcg cgcagggacc
    37321 agccatgaaa ggaagggggc cagcgtctct tcatcaagga tcacgtcgag cagaaaggga
    37381 tcgctgacct ttgcggcttc cgggaccaga ggcagaacgt catactccgt agtactggat
    37441 tgcgtttcat gataccagtg gcttttgccg gtgatgtggc gttgttccat gggcgctcct
    37501 tggtcgtaaa ggaaatcgtt atcctgacgc aaggcgggaa ggggagaaag acggatcggc
    37561 gataacaaat atcagaaagg tataacagag ataacgggcg gcagaacgcc gcccatcttt
    37621 accaacagaa cgattatttc agttcgagtt cgttcattgc agcgatgctg aaaccgccgt
    37681 caacgtggac tacttcaccg gagataccgg cagagagatc ggagcacagg aatgccgcag
    37741 agttacccac atcttcaata gtaactgtac ggcgaatcgg ggtaacggct tcgcaatgag
    37801 ccagcatttt gcggaagtct ttgataccgg aggccgccag agtacggatc ggaccagcag
    37861 agatggcgtt aacacgcaca ccttccggac ccatcgcgtt cgccatatag cgcacgttcg
    37921 cttccagaga cgcttttgcc agacccataa cgttgtagtt cgggatagcg cgctcagcgc
    37981 caaggtagga aagggtcagc agggcagaac ccggattcag catggagcgg caagcttttg
    38041 ccattgcaac gaagctgtag gagctgatgt cgtgggcaat tttgaagcct tcacgggtaa
    38101 cggcgttaac atagtcacca tccagctgat cgccaggtgc aaaaccaata gagtgtacga
    38161 aaccgtcaaa tttcggccaa actttcccca gttcagcgaa catggtgtcg atgctggcat
    38221 cttctgcaac atcgcactgc agaacgatgt cagaacccaa ttgagcggca aattcttcta
    38281 cgcggccttt cagtttgtcg ttctggtagg tgaatgccag ttcagctcct tcgcggtgca
    38341 tcgcctgagc gataccgtag gcgatggaca gtttgctggc aacaccggtt accagaatgc
    38401 gcttaccgga aagaaaaccc atagctttaa tccttattgt tgatgcttgt tgtgcctgaa
    38461 aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga taaacggtta ttataatcaa
    38521 cctggctgtg agtagctata gttaccaggt ccgaccggag caggctgcgg cagggggggc
    38581 gcttttcccc ttaccctaac cctctcccca gaggggcgag gggaccatat tgtgcaaata
    38641 ttgttacccc agcaacaaac aggctcatac agcccctaac ccttttatgg cgatggctgg
    38701 gatggttcag accttgccga atattctcca gcactgtctc catgttttca ccacaacagc
    38761 tattcggctc ggtcggcccc ctcgcccttt cagggtgagg gtgaccggcg gttcagttcc
    38821 tgcagaataa tctccagcac cgcctcctca ttacaatcaa tctcgttatt ccagaaacgc
    38881 agcacggtcc agccctgcga ctcaagccag ctagtgcgcc tggtatcata ggcaactgcc
    38941 aaatcatgct gcccaccatc cagctcaacg actacgcgcg ccgagcagca agcaaaatcg
    39001 agaatgtagc tccccaccgg atgttgacgg cgaaatttga aatcaccaaa acgtcggctg
    39061 cgaagatatc gccagagctt tcgttcctgc aaagtgagat tgcgtcttaa atcacgcgca
    39121 tttgatttta ttttatccat cacctcattc tgacaagatt taatctcttg tcagaatgag
    39181 gtgaataatc tggaaggagg attcagaaaa ttagcgatct ttacgccacg catccgctgt
    39241 caatgcttcg ccaaagtgcc cggcaatcag ccgtttggtg agttcatgca gcggcgatgc
    39301 cagcacatcc gcggtgctgc ctcgctcgac aacctcgccc tgatgcatca ccagcacctg
    39361 gtcgctaatg tgcttcatca ttccgatatg ctgggtaaca taaatatacg aaatgccctg
    39421 tttttcctgt aactccagca tcagattaat caactgcgaa cgcattgaca tatccagtga
    39481 agcgagggct tcatcggcaa taatgacttt tgggcgcaat atcagcgcgc gcgccagacc
    39541 cagacgctgt ttttgtccgg gtgccaacat atgcggatag taactgacgt gatccggtag
    39601 cagcccaacc atacgcatcg tttcaataat ctgtttgcga cgctgttccg gttccaggtc
    39661 ggtgttcagg cgcagtggaa aatccagaat ttgtgagata cgttgacggg gattcaacga
    39721 ggtcgaagga tcctgaaaaa tcatgcgaat acgctgacta cggaaggaat aatcgccaaa
    39781 atgcagcgga tgatcgtcaa tcaataactc gccgctggta ggttctatca ttcccgccag
    39841 catttttgcc agtgtggatt tacccgaacc attctcgcca ataatcgcca gtgtctggcc
    39901 ttcacgtagc gtaaagctca agggttttac cgcttctacg gtctgacgac gaaaccaacc
    39961 ggtccggtag cggaacgttt tacttaaatt acgcacttca agcagcgttt cgatcatctc
    40021 actctttctc catgttcagc gggaaatgac aggcatagag atgatttttc gcccccgtca
    40081 aacgtggcgt cacaatgcat tctcgttgtg catacgggca acgtggcccc agacgacaac
    40141 caatcggtaa ctgttccagc aatgggatag cgccgggtag agtattgagg cgacttttat
    40201 gcggcatcgc gctgccgaag tctggtatcg cgcggatcag cgcctgggta taaggatgat
    40261 gtggcatcgt caccaactcc ttactcggcg cggtttccac tgtttgaccg cagtaaagca
    40321 cgttaatttt atccgcccat tggctaagca tttgtaagtc atggctgata agcaaaatag
    40381 tggtattgct gttttggttg agacgcgtca gcaggcgaaa gatttgcgcc tgggttgttg
    40441 gctccattga gtttgtcggt tcgtcagcaa tcagcagacg cggttgattc gccagtgcaa
    40501 tggctatcat cactttctga cattcacctt cggtcaactc atagggaaaa ctgcgcatcg
    40561 catctttgtg atctttaatc cccacgcggt gcagcagttc aatggcacgg cgtttgcgcc
    40621 agccaaaacg ctgccaccaa cggcctttat aggtccaggc tgggatgttt tgcatcaact
    40681 ggcgacccac acgttctgaa gggtcaagac acgactgcgg ttcctggaaa atcatcgaca
    40741 cgttatggcc aaccagtttg cgccgttcgc gtgcggagag acgcagcaaa tcgatatcat
    40801 caaaacgcat acggtcagca gtaacacgcc agttatcttt attcacccca caaatcgctt
    40861 tcgcaatcaa actcttgccg gaaccggatt caccaacaag accgcggatt tcaccttcgg
    40921 ttaaggtcat acttacccgg tcgacggctt taacccactc atcaccggtt ttaaattcaa
    40981 tggtcaggtt acgaatatcg agtaatggca ttattccacc cccgcaataa tcgcacgacg
    41041 aacgccatcg ccaaggaggt taaccaacaa cacgctaatc ataattgccg cacctggcag
    41101 catgacagtc cacggggcga cataaatcag ttccagcgca tcaccgagca tcgctcccca
    41161 ttcaggcgag gggagttgtg cgccgagatc gagaaagccc agcgcggcga tatcgagaat
    41221 tgccatcgac agtgcgcggg tgatctcggt gaccagcccg gcggtgatgt ttggcatcac
    41281 agcaaaccag agaatattca gcgttgatgc gccatccaga cgggcggcga taacgtactc
    41341 tttttccagt tcgtcatgca ccatgctgta aatcgaacgt accatacgcg gcagcaatgc
    41401 cagccagaca gcaaacatag cgtgggacaa actcggtccg gcaaacgcta caacgataat
    41461 tgccaacagc agcgaaggga tcgccagtaa ggtatccaga atatggttaa gcaccgctga
    41521 gcggaggccg tgtgtcgccc cggcgaaggt gcccagtacc aggccacaga tcgtcgcggc
    41581 aagcgtcacc acaaatgcgc cacccacggt tggcgcagct ccgctcagta aacggcttaa
    41641 aacatcacgt ccgaggtcgt cagtccccag gaagaaagaa acttcgccat agcgcgacca
    41701 tgatggcggc aataattgat aaccgagaaa ttgctggtcg atgccgtaag gcgcaaacca
    41761 gccgccaaaa atacacagta cagccagtcc cgcgcagccg tacaggccga ccatcgcaga
    41821 ggcgtcacta taaaatttgc gccaggcggt acgcagcgtg cccggcgggc gcttttcgct
    41881 gtatacgcta tcgtaaggca taccattcct tatgtttcag agggttagcc atggcaccca
    41941 aaatatcaga aatcacgtta acaataatga ccagtgagcc acacaccatc actccggcgg
    42001 aaatggctgc gtaatcctgc tggcggatgg cgttaattaa ccagcgccct aaacccggcc
    42061 agctaaaaac catttcggtg atcatcgcca gcgtcagcat ggtggaaaac tgtaagccca
    42121 ggcgaggaat aaccggcggt aacgcgttat gcagaacgtg gcgacgcaaa atagtaaagc
    42181 gcgacaaacc gcgggtcgcc gccgctttta catagttctg gtcatacact tcaatagtgc
    42241 tgatacgcat caggcggatc acttcagttg ttggcgcaac cgacagggtg atcacgggca
    42301 atatcatatg gcggattgcg ctcatgatca tttcatcccg ccatggtgag tccgaaagcc
    42361 aggcatcaat caacgcaaaa ccggtaatcg gtttcacttc gtagagcaga tcgaaacgcc
    42421 ctgaaacggg caaccaacct agcgtgagtg aacaaaacag tgtcaacaga agcgccagcc
    42481 agaacaccgg gattgaaaag cccagtaagg cgatggcgtt gatcaagtta tcctgccact
    42541 tatggcgcgt aatcccggca atcatcccca cggggatgcc aacaatcagc gcaaaaccga
    42601 aggcgaggat gcacagttct atcgtcgccg ggaagagctc ttttaactgt tcggcaattg
    42661 gttgaccgtt aatgctggaa acaccaaaat cccagtggat caggccatta aaccagaaca
    42721 cccaggcatt ccacagtgac gcgccttgta acggcgcgtg tggagtgaaa tagctcaagc
    42781 tgaagccaac gaaggtcagc aggaacaagg tgacaatcaa taacaaaatg cggcgtaagg
    42841 tgaagataat catggttttt tcacctcatc ctgtttctcg cgatacaccc cagcaaagga
    42901 ggcgttacca aacgggctaa gtaccagacc tttgatatcg taccgatagg cctgcaaacg
    42961 caatgacgac gccagcggca gaataggtaa ttcctgcgcc agaatactct gcgcttcgtc
    43021 ataggcttca atacgcgctg ccagctgctg cgaggagagc gccttacgca acacgctgtc
    43081 gaatttcgga ttgcaccagt gggcgaggtt ggtctgtgaa tgaattgccg cgcagctcag
    43141 taacggacgg aagaaactgt ccgggtcgtt actgtccgtc gcccaaccgg ataacgtcag
    43201 atcatggctc atatccatca accgcgcctc ctgaaagcga ccttctaccg gcacaatcac
    43261 cacttttacg ccaacctgcg ccatatccgc ctgaatcagt tcggcagttt tcagtggact
    43321 ggggttccac gcctgcgaac gtgtgggcac ccacagtttc agcgttaagt tttccagccc
    43381 caacgccttc aactgttcgc gcgatttcgc cggattgtat tcagtaattt tagcctcgtt
    43441 gtcataggcc cacgaggcgc gcggtaaaat agaggccgcc gtttcagccg taccataata
    43501 gatggattgc atcaggcgct ggttattaat cgccagtgcc agtgcatggc ggacagcggg
    43561 attatttagc ggcggtttgg cggtgttaaa tgccagatag gcgacgttca tcccaggacg
    43621 cagcgttaaa cgcaagcgcg ggtcgtcacg caaaatggat agctggctgg cagcaggcca
    43681 ggccagaacg tcgcattccc cggtcaggag tttcgacaga cgtccggtgc cgccggagcc
    43741 taaatccacc actacctgcg gcattaacgg tttaccgcgc cagaagtcat catgacgttg
    43801 taggcgaata aattgcccgg cgcggtattc cgacaactga tacggaccgg tgccgaccgg
    43861 ttgacggtcg agttgctcct ggcgatcttc tttctctaac ttccgggcat attctgccga
    43921 catgaccgaa gcataatggg ttgcgaggtg ccacaaaaaa gaagcatccg gctgggcgag
    43981 tcggaactca acggtatgat tatccagttt gcggacgctt ttcacgttat cggcaaattg
    44041 caggctgtcg aagtagggga agttgctgcc gttgacgtta tgccacgggt tgttgcggtc
    44101 aaaaattcgc tggaaggtaa acaccacatc gtcggcattc attttacgag tgggagtaaa
    44161 ccagtcggtt ttttgaaacg gaacatcgcg acgcaggtgg aagcgatagg tcgcgccgtt
    44221 gtcgagtact tcccagcttt cggcaagttc cggcatcagg cgataggtat agggatcgac
    44281 atccagcagt cgatcataaa actgggcggc aagggtatcg acaattaacc cactgctcgc
    44341 tttggatggg ttaaaggtgt tgacttgccc gctgacgcaa tagacaaaac cgctgtcgcg
    44401 gatatcagca tgcggggggg attcaggcgc ggcgattgcc tgaccactca caagtccagc
    44461 aatcaccaaa agagacgata atacctggcg cataatatta agggatttta tgtaaagagg
    44521 ctatcttact aatatttaat gacatttgcc attaccgttt gtgttcaggg gtcgtaatga
    44581 gggccgtgtg gttggtcgtt gaacaggtgg cgtcgccatc tgttcaacat tcgtacccgt
    44641 gatttctcta taactataac tcacagaaca acttagcgag gagcagagcg gtacgatcgt
    44701 gagaaaccca ttaaagcacg ctgcagtgcg tgtcattgtt agccggatgc ggcgtgaacg
    44761 ctttatccgg acaacgatac tgaccgatcg tctgcaatta aataattact cattacccca
    44821 ttgattcaaa aactctgcga tctcatcaat gcgtacggga ttaatccccg cttcagcagc
    44881 catttcatgt tgggcttctt cgctgatctc ttcattgttc atcaaacggg tgagcagtaa
    44941 ctggaagtag tgggccagcg gtgtgcgttc agcctctgcg acaggctctg cgcattcggt
    45001 cgcgtactca tcggcaatat caaaatattt tagcgggata tcgtggttca ttatttgccc
    45061 ctggggttaa tgcgctaagc ataagggcgg atgatagcac tcgtgatccg ggtcatacat
    45121 ctttttatag ttaatggcgc ggcgcgttac cctcaggcag gataatgcgc cgcgcatccg
    45181 gctattacgt ttcgctcacc ggcgttctgt caccttcgta cagcggcact ttgcgatagc
    45241 ggcggatcag ataccacaaa tatgcgccgc ctaacgaagc ccataccaga ccgagtgtca
    45301 gtgacgttga ctctaggtta acccacagca cacccaccgt cagcgcgcca accagcggca
    45361 tcagcaaata gtggaagtga tccttccagc ttttattcat tcctttacgc cgccagaaat
    45421 gattaaacac cgacagattc acgaaggtaa acgccaccag agcaccgaag ttaatcagtg
    45481 ctgtcgcggt gactaaatca aagaacagcg ccgacagcgc gacaatcccg accataatga
    45541 cgttgagtgc cggagtccgc cattttgggt gcacatagcc aaacacgcgc tccggaaaca
    45601 cattgtcacg ccccatcaca tacagcagac gcgacacgct ggcatgtgaa gccaggccag
    45661 acgctaatgt gttcacaaac gtggtgcaga ggaaaatcga ctgaaagagc ttgccgccga
    45721 cgtacagcgc aatttcaggc agtgcggcgt ccgggtcttt aaagcggctg atatcgggaa
    45781 agaacagctg cataaagaac gacgccgcga taaagataac gccgccgtat accgccgtca
    45841 ggaagatggc tttcgggatc acgcgtgcgg cgtccggcgt ctcttccgaa agcgtggtca
    45901 ccgcatcgaa accgaggaac gagaaacaga caatcgtcgc accggtaata atcgggatca
    45961 ggtgcgcgtt ctcgctgata aacggctgaa gtgaccagac ggtgccaacg ccttctcctt
    46021 tatgcagtcc ctgaaccacc aggaagataa acaccaccat gatggagatt tgcaccagta
    46081 caaacagggt attgaagtta gcgaccaggt tgacgctctt cagattcgcg gcggttaaaa
    46141 tggcgacgaa ggttaccacc cacacccacg gcggcacttc cgggaagagg gcggagagat
    46201 agattttcgc caacaaaacg ttgatcattg gcaaaaagag ataatccagc agtgatgacc
    46261 agccgaccat aaatccgacg tgcgggttaa tcgacttttg cgcgtaggta taggccgaac
    46321 cggcctccgg aaactggcga accagtttgc cgtagctgat agcggtaaac agcacgcccg
    46381 ccagcgccag caaataggac gccggaacgt gaccgtcgct aatgccggag acaataccaa
    46441 aagtatcaaa tacggtcatc ggcgtgagat aggccagacc catcattacc acctgccaca
    46501 atttcagtga ttttcgcaga cgggttttgc cgggttgcgc agcaatattc agtggtgaat
    46561 taatagccat aattgctgcc tcccctgcgt aataaggttt gcggtcgtgt cgacctgaaa
    46621 tcaaacctgt ttagcaaggg aagcaaaggg ggcggagtcc agtcagtagc aggaggggcg
    46681 catacgcagc ctccctcgcc atgcggggat ttaatcatcg gcatcatctc atttcctcgt
    46741 cacagtctta atcgttaacg gaagcccgtt gccgcctgcg ctccataacg cgacatccgg
    46801 gaaatgtctg gattaccagc aaatgtcagg cctggctccg ctcaggccga tgaaacaacc
    46861 ccgcaagggg aattacgcgt ttttcaacat ccactcaatt tctgtttctg tgatgaggcg
    46921 ctcaaactgc aacagctcat catttttaca ggcgtgatac acatggcaaa accgttcgcc
    46981 taaatagcgg cgcaggtgat cattctcgat aaactcaccc agggcatcgc tctggcgaat
    47041 cgggaagggt aagccttcct gttccagccc gttgccttcg acttcttcct gcaacggcag
    47101 ctcgttatca aggccatgca aaataccggc aaaaatcgct gccatcacca gatatgggtt
    47161 ggcatcggca cccgccacgt gatattccac gcggtgatta tgacggtcac cgcacggaat
    47221 acgcagggcg acggtgcggt tgttatggcc ccacgacgcc tgcgtcggca catacattcc
    47281 cggctggaag cggcgatacg agttcacgtt tggtgccagc aacgccatcg acgacggcat
    47341 caggtcaatc atcccggcga gcatcttttt cagcagcggc gaatcttcgc cttctgtgtc
    47401 agaaagcaca ttctcgccac gattattttg catactgata tggatatgca tcccgctgcc
    47461 cgcgtgctct tcatacggct tcgccataaa agtggcgtgc atcttatgct tttctgccat
    47521 cagacgcact aggcgtttta gtgccagcgc atcatcgcag gcttccagca cgttatcagt
    47581 atggtaaagg ttgatttcaa actgacccgg cgaggcctca gcgaccgcgc catctgccgg
    47641 aatcagctgc aactgcgcca gttcatcaat atcattgagc acgtcggcga agtggttgag
    47701 gttatcaacg gagtaaacct ggctttgtgt attgcggtca tcggtgccag gcgcgcaggg
    47761 cggttgcaga tccccttcag cgtcgcgctg gcgatccagt aaatagaact ccagctctac
    47821 cgctacaacc gggaacaatc cgcgctggcg cagctgctgc cacaggcggt taagaacgtt
    47881 ccgcggctca acgtcaaagg gagcgccatc ttcatcgacc atggtcagga gcatctgacc
    47941 gataaactct ggatcggcgg cagaaggagt taaggaaccg agaacaggaa cacaggtacg
    48001 atccggctcg cccatttcct gacccagacc tgcttcttct actacgttgc ccagaatatc
    48061 cattgcaaat accgaggccg ggaaataaca ccctttctcg agcttcttca ggcttgaaac
    48121 agggatacgt tttccacgga agcaaccatt caaatcagta agtaaaacat caacatattg
    48181 cgtattcggg tagcgctcca ggtagcgttt cacttcctgc gtaaaggcgc taccccgcct
    48241 ctcttctgac tgctgaacaa agttctctac ttcaacgata ttggtttcca tgattcttcg
    48301 cctttggttt gttttccgct cgttatcaaa gcgtaaaata taatgaccat cattcgaatc
    48361 tgtatgcaaa ccaaatgttt gtcaaatgtt aaattgagtt tgcaaaaatg aaaatccact
    48421 gctagattga aaaaatattg aacataaagg tcatttaaag cgcagtaatg gcgataattt
    48481 agtccatttt gtgagattga gcatggaaaa tataatgaac aatccggtta tcggtgtcgt
    48541 aatgtgcagg aacaggctta agggtcatgc gacccagact ctgcaagaaa agtacctgaa
    48601 tgccatcatc cacgcaggcg gcttgccaat tgcgctacca catgcgctgg cggaaccgtc
    48661 gttacttgaa caacttttgc cgaaactcga tggcatttat cttcctagta gtcccagcaa
    48721 tgtgcagccg cacctatatg gtgaaaacgg cgatgagcct gacgccgatc ccgggcgtga
    48781 tcttctgagc atggcgataa ttaatgccgc actcgaaagg cgcatcccca ttttcgccat
    48841 ctgccggggt ttacaagaac tggtggtggc aaccggtggt tcgttgcatc gcaagctgtg
    48901 cgaacagcct gaattgctgg aacatcggga agatcccgaa ctgccggtgg aacagcaata
    48961 tgcaccgtcg catgaagttc aggttgaaga ggggggatta ctgtctgcgt tgttacctga
    49021 atgtagcaac ttttgggtaa actctctaca tggacaaggg gcgaaggtcg ttagcccacg
    49081 gttgcgtgtt gaagctcgct cgccggatgg cttagttgag gcggttagcg tcatcaatca
    49141 tccttttgcg ctgggcgtac agtggcaccc ggaatggaac agtagcgagt acgcgctttc
    49201 gcgtatattg ttcgagggct ttatcaccgc ttggcagcac catatcgctg aaaaacagcg
    49261 actctgacca ctacagttta aggaaatgca aatatgagtg atgagggact ggcgccagga
    49321 aaacgcttgt cggaaatccg ccagcagcag gggctttcac aacgtcgtgc cgccgaactc
    49381 tccgggctga ctcacagtgc tatcagtacg atagaacaag ataaagtcag ccctgccatc
    49441 agtacgctgc aaaagctgct gaaggtgtat ggtctgtcac tctcggaatt cttttccgag
    49501 ccggaaaaac ctgatgagcc gcaggtcgtc attaatcagg acgacttaat tgagatgggc
    49561 agtcagggtg tgtcaatgaa gctggttcat aacggtaacc cgaatcgcac gctggcgatg
    49621 atctttgaaa cgtaccagcc gggcacaacc actggggaaa gaattaagca tcagggtgag
    49681 gaaataggca cagtactgga aggtgaaatt gttctgacga ttaatggtca ggattaccac
    49741 ctcgtcgcgg ggcaaagtta tgccattaat accggcatcc cgcacagttt cagtaatacg
    49801 tcggcaggta tttgccgaat tatcagcgcc catacgccca ccacgtttta atctttttgt
    49861 tctgtaagcc gggtaagcgc aatgcgcccg gcaatccata aagaatgtcg ccagaaataa
    49921 acgggcatgc agcccgggga tctctgcgcc ctgacgttca caaactgcat atatctgata
    49981 gacgtgaaac aggagtcata atgaattttc atcatctggc ttactggcag gataaagcgt
    50041 taagtctcgc cattgaaaac cgcttattta ttaacggtga atatactgct gcggcggaaa
    50101 atgaaacctt tgaaaccgtt gatccggtca cccaggcacc gctggcgaaa attgcccgcg
    50161 gcaagagcgt cgatatcgac cgtgcggtga gcgcagcacg cggcgtattt gaacgcggcg
    50221 actggtcact ctcttctccg gctaaacgta aagcggtact gaataaactc gccgatttaa
    50281 tggaagccca cgccgaagag ctggcactgc tggaaactct cgacaccggc aaaccgattc
    50341 gtcacagtct gcgtgatgat attcccggcg cggcgcgcgc cattcgctgg tacgccgaag
    50401 cgatcgacaa agtgtatggc gaagtggcga ccaccagtag ccatgagctg gcgatgatcg
    50461 tgcgtgaacc ggtcggcgtg attgccgcca tcgtgccgtg gaacttcccg ctgttgctga
    50521 cttgctggaa actcggcccg gcgctggcgg cgggaaacag cgtgattcta aaaccgtctg
    50581 aaaaatcacc gctcagtgcg attcgtctcg cggggctggc gaaagaagca ggcttgccgg
    50641 atggtgtgtt gaacgtggtg acgggttttg gtcatgaagc ggggcaggcg ctttcgcgtc
    50701 ataacgatat cgatgccatc gcctttaccg gctccacgcg tacgggtaaa caactgctga
    50761 aagacgcggg tgatagcaac atgaaacgtg tctggctgga agcgggcggc aaaagcgcca
    50821 acatcgtttt cgccgattgt ccggatctgc aaaaagcggc aagcgccacc gccgcaggca
    50881 tcttctacaa ccagggccag gtgcgcatcg ccggaacgcg tttgttactg gaagagagca
    50941 tcgccgatga attcttagcc ctgttaaaac agcaggcgca aaactggcag ccgggccatc
    51001 cacttgatcc cgcaaccacc atgggcacct taatcgacag cgctcacgct gactcggtac
    51061 acacctttat tcgtgaaggc gaaggcaagg ggcaactgct gctggatggt cgcaacgccg
    51121 ggctggcagc ggccatcggc ccgaccatct tcgtggatgt ggacccgaat gcgtccttaa
    51181 gccgcgaaga gattttcggt ccggtgctgg tggtcacccg tttcacatca gaagatcagg
    51241 cgctgcagct tgccaacgac agccagtacg gccttggcgc ggcggtatgg acgcgcgacc
    51301 tctcccgcgc gcaccgcatg agccgacgcc tgaaagccgg ttccgtcttc gtcaataact
    51361 acaacgacgg cgatatgacc gtgccgtttg gcggctataa gcagagcggc aacggtcgcg
    51421 acaaatccct gcatgccctt gaaaaattca ctgaactgaa aaccatctgg ataagcctgg
    51481 aggcctgaaa tgaccgaaca taccagcagt tactacgccg ccagtgcgaa taaatatgca
    51541 ccattcgaca cactgaatga gtcaatcacc tgcgacgttt gcgtggttgg cggcggctat
    51601 accgggctct cctccgcgct gcatctggcg gaagcgggct ttgacgtagt ggttctcgaa
    51661 gcctcacgca tcggctttgg cgcaagcggg cgcaatggcg ggcagcttgt gaactcctac
    51721 agccgcgaca tcgacgtgat cgaaaaaagc tacggcatgg acaccgcccg tatgctcggc
    51781 agcatgatgt tcgagggtgg cgagatcatc cgcgaacgta tcaaacgtta tcagattgac
    51841 tgcgactacc gccccggcgg tctgtttgtg gcgatgaatg ataaacaact cgccacactc
    51901 gaagagcaga aagagaactg ggtacgctac ggcaataaac agctggagct gctggacgtc
    51961 aacgctattc gccgcgaggt cgccagcgat cgctacactg gtgcgctgct ggatcacagc
    52021 ggtgggcata ttcacccgct aaacctcgcc attggtgaag ccgatgccat ccgcctgaac
    52081 ggcgggcgcg tgtatgagct ttccgccgtg acgcagatcc agcacaccac gccagccgtg
    52141 gttcggactg ccaatggtca ggtgacggcg aagtatgtga ttgtcgccgg gaatgcgtat
    52201 ctgggcgata aagtagagcc ggaactagcg aaacgcagca tgccgtgcgg cacgcaggtg
    52261 atcaccaccg aacggctgtc ggaagattta gcccgttcgc tgatcccgaa aaactactgt
    52321 gtggaagact gtaactatct gcttgattac taccgtctta ccgccgacaa ccgcctgctg
    52381 tacggcgggg gtgtagtgta tggtgcacgc aacccggatg acgttgagcg tcttgtggta
    52441 ccgaaactac taaaaacctt ccctcagttg aagggcgtga aaattgatta ccgctggacg
    52501 ggcaacttcc tgctgacctt gtcgcacatg ccgcagttcg gccgccttga taccaacatc
    52561 tattacatgc agggttacag cggccacggc gtcacctgta cccatcttgc cgggcgtttg
    52621 attgctgaac tgctgcgcgg tgacgccgag cgtttcgatg ccttcgccaa tctgccgcat
    52681 tacccgttcc ccggcgggcg cacgctgcgc gtgccattta ccgcgatggg cgcggcttat
    52741 tacagcctgc gcgatcgtct gggcgtttaa tttccgatta acagtgaaga gtcaaaaggt
    52801 gtgaaacatg agcaacaatg aattccatca gcgtcgtctt tctgctaccc cacgcggggt
    52861 tggcgtgatg tgtaacttct tcgcccagtc ggcagaaaac gctacgctga aggacgtaga
    52921 gggcaacgaa tacatcgatt tcgccgcagg cattgcggtg ctgaataccg ggcatcgcca
    52981 ccctgatctg gtcgcggcgg tggagcagca attgcaacag tttacccaca cggcgtatca
    53041 gattgtgccg tacgaaagct acgtcacgct ggcggagaaa attaacaccc ttgccccagt
    53101 gagcgggcag gctaaaaccg cattcttcac taccggcgcg gaagcggtgg aaaacgcggt
    53161 gaaaattgct cgcgcccata ccggacgacc tggcgtgatt gcgtttagcg gtggcttcca
    53221 cggtcgtaca tatatgacca tggcgctgac cggaaaagtt gcgccgtaca aaatcggctt
    53281 cggcccgttc cctggttcgg tgtatcacgt accttatccg tcagatttac acggcatttc
    53341 aacacaggac tccctcgacg ccatcgaacg cttgtttaaa tcagacatcg aagcgaagca
    53401 ggtcgcggcc attattttcg aaccggtgca gggcgagtgc ggtttcaacg ttgcgccaaa
    53461 agagctggtt gccgccattc gccgcctgtg cgacgagcac ggtattgtga tgattgccga
    53521 tgaagtgcaa agcggctttg cgcgtaccgg taaactgttt gccatggatc attacgccga
    53581 taagccggac ttaatga
//
LOCUS       DAJTRN010000016        53046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRN010000016 DAJTRN010000000
VERSION     DAJTRN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(11..322)
                     /locus_tag="OO328_000986"
     CDS             complement(11..322)
                     /locus_tag="OO328_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5929641.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO328_000987"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO328_000987"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO328_000988"
     CDS             701..1054
                     /locus_tag="OO328_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5929642.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO328_000989"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO328_000989"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5929643.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO328_000990"
     CDS             complement(2810..3280)
                     /locus_tag="OO328_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5929644.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO328_000991"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO328_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO328_000992"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO328_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5929645.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO328_000993"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO328_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5929646.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO328_000994"
     CDS             complement(5283..8432)
                     /locus_tag="OO328_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5929647.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO328_000995"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO328_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5929648.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO328_000996"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO328_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5929649.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO328_000997"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO328_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5929650.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO328_000998"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO328_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5929651.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO328_000999"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO328_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5929652.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO328_001000"
     CDS             14911..15288
                     /locus_tag="OO328_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5929653.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO328_001001"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO328_001001"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5929654.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO328_001002"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO328_001002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5929655.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO328_001002"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO328_001003"
     CDS             18099..18428
                     /locus_tag="OO328_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5929656.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO328_001004"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO328_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5929657.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO328_001005"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO328_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5929658.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO328_001006"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO328_001006"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5929659.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO328_001007"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO328_001007"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5929660.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO328_001008"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO328_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO328_001009"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO328_001009"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5929661.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO328_001010"
     CDS             complement(25481..27157)
                     /locus_tag="OO328_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5929662.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO328_001011"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO328_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5929663.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO328_001012"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO328_001012"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5929664.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO328_001013"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO328_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5929665.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO328_001014"
     CDS             complement(31205..31999)
                     /locus_tag="OO328_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5929666.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO328_001015"
     CDS             32137..32478
                     /locus_tag="OO328_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5929667.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO328_001016"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO328_001016"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5929668.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO328_001017"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO328_001017"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5929669.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO328_001018"
     CDS             36394..37686
                     /locus_tag="OO328_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5929670.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="OO328_001019"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="OO328_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5929671.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="OO328_001020"
     CDS             complement(38219..38677)
                     /locus_tag="OO328_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5929672.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO328_001021"
     CDS             complement(38674..39591)
                     /locus_tag="OO328_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5929673.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO328_001022"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO328_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5929674.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO328_001023"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO328_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5929675.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO328_001024"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO328_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5929676.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO328_001025"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO328_001025"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5929677.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO328_001026"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO328_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO328_001027"
     CDS             43550..44236
                     /locus_tag="OO328_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5929678.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OO328_001028"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OO328_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO328_001029"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO328_001029"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5929679.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO328_001030"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO328_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5929680.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="OO328_001031"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="OO328_001031"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5929681.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="OO328_001032"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="OO328_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5929682.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="OO328_001033"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="OO328_001033"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5929683.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53046
                     /locus_tag="OO328_001034"
     CDS             52939..>53046
                     /locus_tag="OO328_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5929684.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMF"
BASE COUNT        12786 a        13655 c        13965 g        12640 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tactgtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgttt
//
LOCUS       DAJTRN010000017        49830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRN010000017 DAJTRN010000000
VERSION     DAJTRN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1224
                     /locus_tag="OO328_001035"
     CDS             <1..1224
                     /locus_tag="OO328_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5929685.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO328_001036"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO328_001036"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5929686.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO328_001037"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO328_001037"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5929687.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO328_001038"
     CDS             2714..3427
                     /locus_tag="OO328_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5929688.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO328_001039"
     CDS             complement(3399..4076)
                     /locus_tag="OO328_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5929689.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OO328_001040"
     CDS             complement(4070..4747)
                     /locus_tag="OO328_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5929690.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OO328_001041"
     CDS             complement(4735..5118)
                     /locus_tag="OO328_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5929691.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OO328_001042"
     CDS             complement(5134..5442)
                     /locus_tag="OO328_001042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929692.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OO328_001043"
     CDS             complement(5443..6021)
                     /locus_tag="OO328_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5929693.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OO328_001044"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="OO328_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OO328_001045"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OO328_001045"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5929694.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OO328_001046"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OO328_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5929695.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OO328_001047"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OO328_001047"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5929696.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OO328_001048"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OO328_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5929697.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OO328_001049"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OO328_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5929698.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OO328_001050"
     CDS             12291..13031
                     /locus_tag="OO328_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5929699.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OO328_001051"
     CDS             13198..14148
                     /locus_tag="OO328_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5929700.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO328_001052"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO328_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO328_001053"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO328_001053"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5929701.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO328_001054"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO328_001054"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5929702.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO328_001055"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO328_001055"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5929703.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO328_001056"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO328_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5929704.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="OO328_001057"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="OO328_001057"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5929705.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="OO328_001058"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="OO328_001058"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5929706.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="OO328_001059"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="OO328_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5929707.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="OO328_001060"
     CDS             23768..23827
                     /locus_tag="OO328_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5929708.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="OO328_001061"
     CDS             complement(23928..24530)
                     /locus_tag="OO328_001061"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5929709.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="OO328_001062"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO328_001062"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO328_001063"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO328_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5929710.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="OO328_001064"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="OO328_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5929711.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="OO328_001065"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="OO328_001065"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5929712.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="OO328_001066"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="OO328_001066"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5929713.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="OO328_001067"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="OO328_001067"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5929714.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="OO328_001068"
     CDS             complement(29676..29759)
                     /locus_tag="OO328_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929715.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="OO328_001069"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="OO328_001069"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5929716.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="OO328_001070"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="OO328_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5929717.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="OO328_001071"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="OO328_001071"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5929718.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="OO328_001072"
     CDS             34816..35559
                     /locus_tag="OO328_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5929719.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO328_001073"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO328_001073"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5929720.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="OO328_001074"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="OO328_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="OO328_001075"
     CDS             37568..38227
                     /locus_tag="OO328_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5929721.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO328_001076"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO328_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5929722.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="OO328_001077"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="OO328_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5929723.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="OO328_001078"
     CDS             39893..40300
                     /locus_tag="OO328_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5929724.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO328_001079"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO328_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5929725.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="OO328_001080"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="OO328_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5929726.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="OO328_001081"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="OO328_001081"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5929727.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="OO328_001082"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="OO328_001082"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5929728.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="OO328_001083"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="OO328_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5929729.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="OO328_001084"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="OO328_001084"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5929730.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO328_001085"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO328_001085"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5929731.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="OO328_001086"
     CDS             47705..47776
                     /locus_tag="OO328_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929732.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="OO328_001087"
     CDS             complement(47773..47898)
                     /locus_tag="OO328_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929733.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO328_001088"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO328_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5929734.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12451 c        13406 g        11922 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caagcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattgattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtca
//
LOCUS       DAJTRN010000018        49492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRN010000018 DAJTRN010000000
VERSION     DAJTRN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(41..1219)
                     /gene="setB"
                     /locus_tag="OO328_001089"
     CDS             complement(41..1219)
                     /gene="setB"
                     /locus_tag="OO328_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5929735.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1587..2717
                     /gene="fruB"
                     /locus_tag="OO328_001090"
     CDS             1587..2717
                     /gene="fruB"
                     /locus_tag="OO328_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5929736.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2717..3655
                     /gene="fruK"
                     /locus_tag="OO328_001091"
     CDS             2717..3655
                     /gene="fruK"
                     /locus_tag="OO328_001091"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5929737.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3672..5363
                     /gene="fruA"
                     /locus_tag="OO328_001092"
     CDS             3672..5363
                     /gene="fruA"
                     /locus_tag="OO328_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5929738.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5787..6728
                     /locus_tag="OO328_001093"
     CDS             5787..6728
                     /locus_tag="OO328_001093"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5929739.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6716..7654
                     /gene="psuG"
                     /locus_tag="OO328_001094"
     CDS             6716..7654
                     /gene="psuG"
                     /locus_tag="OO328_001094"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5929740.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7748..8998
                     /locus_tag="OO328_001095"
     CDS             7748..8998
                     /locus_tag="OO328_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5929741.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9069..9763)
                     /gene="yeiL"
                     /locus_tag="OO328_001096"
                     /pseudo
     CDS             complement(9069..9763)
                     /gene="yeiL"
                     /locus_tag="OO328_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9894..10835
                     /gene="rihB"
                     /locus_tag="OO328_001097"
     CDS             9894..10835
                     /gene="rihB"
                     /locus_tag="OO328_001097"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5929742.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10935..12185
                     /locus_tag="OO328_001098"
     CDS             10935..12185
                     /locus_tag="OO328_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5929743.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12241..13329)
                     /locus_tag="OO328_001099"
     CDS             complement(12241..13329)
                     /locus_tag="OO328_001099"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5929744.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13332..14189)
                     /gene="nfo"
                     /locus_tag="OO328_001100"
     CDS             complement(13332..14189)
                     /gene="nfo"
                     /locus_tag="OO328_001100"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5929745.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14263..15312)
                     /locus_tag="OO328_001101"
     CDS             complement(14263..15312)
                     /locus_tag="OO328_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5929746.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15411..16292
                     /locus_tag="OO328_001102"
     CDS             15411..16292
                     /locus_tag="OO328_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5929747.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16498..17967
                     /gene="lysP"
                     /locus_tag="OO328_001103"
     CDS             16498..17967
                     /gene="lysP"
                     /locus_tag="OO328_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5929748.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18259..20250
                     /gene="cirA"
                     /locus_tag="OO328_001104"
                     /pseudo
     CDS             18259..20250
                     /gene="cirA"
                     /locus_tag="OO328_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20282..21117)
                     /gene="yeiG"
                     /locus_tag="OO328_001105"
                     /pseudo
     CDS             complement(20282..21117)
                     /gene="yeiG"
                     /locus_tag="OO328_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21375..22043
                     /gene="folE"
                     /locus_tag="OO328_001106"
     CDS             21375..22043
                     /gene="folE"
                     /locus_tag="OO328_001106"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5929749.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22060..23136
                     /locus_tag="OO328_001107"
     CDS             22060..23136
                     /locus_tag="OO328_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5929750.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23278..24318
                     /gene="galS"
                     /locus_tag="OO328_001108"
     CDS             23278..24318
                     /gene="galS"
                     /locus_tag="OO328_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5929751.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24598..25596
                     /gene="mglB"
                     /locus_tag="OO328_001109"
     CDS             24598..25596
                     /gene="mglB"
                     /locus_tag="OO328_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5929752.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25657..27177
                     /gene="mglA"
                     /locus_tag="OO328_001110"
     CDS             25657..27177
                     /gene="mglA"
                     /locus_tag="OO328_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5929753.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27193..28203
                     /gene="mglC"
                     /locus_tag="OO328_001111"
     CDS             27193..28203
                     /gene="mglC"
                     /locus_tag="OO328_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5929754.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28239..29452)
                     /gene="preA"
                     /locus_tag="OO328_001112"
                     /pseudo
     CDS             complement(28239..29452)
                     /gene="preA"
                     /locus_tag="OO328_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29446..30684)
                     /gene="preT"
                     /locus_tag="OO328_001113"
     CDS             complement(29446..30684)
                     /gene="preT"
                     /locus_tag="OO328_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5929755.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30878..31117)
                     /locus_tag="OO328_001114"
     CDS             complement(30878..31117)
                     /locus_tag="OO328_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5929756.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31120..31839)
                     /gene="sanA"
                     /locus_tag="OO328_001115"
     CDS             complement(31120..31839)
                     /gene="sanA"
                     /locus_tag="OO328_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5929757.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31989..32873)
                     /gene="cdd"
                     /locus_tag="OO328_001116"
     CDS             complement(31989..32873)
                     /gene="cdd"
                     /locus_tag="OO328_001116"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5929758.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33003..33698)
                     /locus_tag="OO328_001117"
     CDS             complement(33003..33698)
                     /locus_tag="OO328_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5929759.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33695..34093)
                     /locus_tag="OO328_001118"
     CDS             complement(33695..34093)
                     /locus_tag="OO328_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5929760.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34332..35279
                     /gene="dusC"
                     /locus_tag="OO328_001119"
     CDS             34332..35279
                     /gene="dusC"
                     /locus_tag="OO328_001119"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5929761.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35650..35733)
                     /gene="yohP"
                     /locus_tag="OO328_001120"
     CDS             complement(35650..35733)
                     /gene="yohP"
                     /locus_tag="OO328_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5929762.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35957..37393
                     /gene="mdtQ"
                     /locus_tag="OO328_001121"
                     /pseudo
     CDS             35957..37393
                     /gene="mdtQ"
                     /locus_tag="OO328_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37446..38207
                     /locus_tag="OO328_001122"
                     /pseudo
     CDS             37446..38207
                     /locus_tag="OO328_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38324..38902)
                     /locus_tag="OO328_001123"
     CDS             complement(38324..38902)
                     /locus_tag="OO328_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5929763.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39072..39659
                     /locus_tag="OO328_001124"
     CDS             39072..39659
                     /locus_tag="OO328_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5929764.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39833..40765
                     /gene="pbpG"
                     /locus_tag="OO328_001125"
     CDS             39833..40765
                     /gene="pbpG"
                     /locus_tag="OO328_001125"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5929765.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40803..42518)
                     /gene="dld"
                     /locus_tag="OO328_001126"
     CDS             complement(40803..42518)
                     /gene="dld"
                     /locus_tag="OO328_001126"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5929766.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42714..45011
                     /gene="bglX"
                     /locus_tag="OO328_001127"
     CDS             42714..45011
                     /gene="bglX"
                     /locus_tag="OO328_001127"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5929767.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45263..46180
                     /gene="osmF"
                     /locus_tag="OO328_001128"
     CDS             45263..46180
                     /gene="osmF"
                     /locus_tag="OO328_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5929768.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46187..47344
                     /gene="yehY"
                     /locus_tag="OO328_001129"
     CDS             46187..47344
                     /gene="yehY"
                     /locus_tag="OO328_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5929769.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47337..48263
                     /gene="yehX"
                     /locus_tag="OO328_001130"
     CDS             47337..48263
                     /gene="yehX"
                     /locus_tag="OO328_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5929770.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48268..48999
                     /gene="yehW"
                     /locus_tag="OO328_001131"
     CDS             48268..48999
                     /gene="yehW"
                     /locus_tag="OO328_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5929771.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48980..49087)
                     /locus_tag="OO328_001132"
     CDS             complement(48980..49087)
                     /locus_tag="OO328_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5929772.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49147..49492)
                     /locus_tag="OO328_001133"
                     /pseudo
     CDS             complement(49147..49492)
                     /locus_tag="OO328_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11960 a        12194 c        13287 g        12051 t
ORIGIN      
        1 atgacaaggt catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta
       61 cccaaaacag agtggcgata atcatcacca tcgcaaacca gaacacagcg tgataattcc
      121 agatctcggc gacgatgccc gccactgatc ctgcgatgat ccagcccacg cgcgacgtgt
      181 tggtatagag cgtggtggct gaacctgcct gaccgggcat caaatcctga aaatagagca
      241 tcccgatgcc gcccagaatg ccaataaaaa tagcatttag cagctgcaag cccaacagaa
      301 tggcaggtga atgcgccatc agcattcctg cgtaaaaaca gacgccaccc acggcagcaa
      361 cgcgcattaa gaaacgctta cccagacgtt tggcgaaata tccggcaatc aacatcgtcg
      421 ggatttccag cccggcggcg gtccccatca tcacaccggc cagtttctcg ggaagatgca
      481 gttcgttgat aataaatagc ggcatgttga tgatgtacag gctgttcgag ccccacatca
      541 atgtacaaat gacaaacagc agtagcgtat cgcgacggtt acggcgcggc gcttcaaccg
      601 tgccggtcgc cagcggaagc tctttttgca tcgacggtaa aaacagccac accatcacgc
      661 cgcaaacaat aaacgctacc gctgcgctca gatacattac cgtaaagctg aaacccatcg
      721 ctaacgcata agccagcggt gggccaatga cccatgccag tgaaacctga gcgcgtaaaa
      781 aagagctgaa catcaccgcc tcacgtccgg ttttgtcggc atgttcacgg gcaagggcaa
      841 acatttgcgg gttagcggtc gagccaaagc tgctaagaaa gacgccaacg aatagcaaaa
      901 caaagtagtt gcgattccaa gcaaaaaggg tgcaggccag cacgcctaac aggcagcaaa
      961 agacaatcag cgatttgcga tcgccgcgct tatcagagcg cccagcgaga aactgactta
     1021 ccagaatccc aatgacagcg ctgccggtaa agaagaatcc caccatcgcc ggacgggcat
     1081 gtacttcatc ggtaaggaaa atactgagtg tcggggtttg cagagcaccc gcaataccgg
     1141 tgagaaaggc aacgattaaa aacgccgtcg aggtcaggtc aaacgatttc gcgctggaga
     1201 ctgcggggga gttatgcatg ctggtcgatc actggaagag gaagacgcgg agtttacgct
     1261 gcttgtcaga aaataaacag acagcgaaat caaaagcgcc acgaataatc aaaatgccat
     1321 ttcactgttg ctgattggct gctgacgctg ctgaaatgaa attgctgata acggattttc
     1381 ccatcagcaa ttaggaaaaa tgacaaaaaa ttgtgcagca catcaaactt ttgctcataa
     1441 ctttacggct ttccttgcgt gctgaaacag aagtattatg ctttcttgaa acgtttcagc
     1501 gcgatcttgt ctttaaccct aagctaggtt ggcgcttttt ttctcataga ggctgaatcg
     1561 tttcaattca gcaagagagg agaactatgt tccagttatc cgtacaggac atccatccgg
     1621 gcgaaaaggc cggagacaaa gaagaggcga ttcgccaggt cgctgcggcg ctggtgcagg
     1681 ccggtaatgt agcagaaggc tacgtcaatg gcatgctggc gcgcgaacag caaacctcaa
     1741 cgttcctcgg caatggtatt gctattccac acggcactac cgacacccgc gatcaggtgc
     1801 tgaaaaccgg cgttcaggta tttcagttcc cggaaggcgt cacctggggt gacggtcagg
     1861 tagcgtacgt ggcaatcggt attgctgcca gctcggataa acatctgggc ctgctacgcc
     1921 agctgaccca cgtactgagc gatgattccg ttgctgaaca actgaagtca gcaacaacag
     1981 cagaagaact tcgcgcatta ctgatgggcg aaaagcagag tgagcagctg aagctcgaca
     2041 acgaaatgct gacgctggat atcgtcgcca gcgatctgct gactcttcag gcgctgaacg
     2101 ctgcgcgtct gaaagaggcg agggcagttg acgccacttt cgtcaccaaa gccatcaatg
     2161 aacaaccgct gaatctcgga cagggtatct ggctgagcga tagcgccgaa ggcaatctgc
     2221 gtagtgcgat tgcggtaagc cgtgcggcaa atgcttttga tgtggacggc gaaacggcag
     2281 ccatgctggt gagtgtggcg atgaatgacg atcagcccat cgcggttctt aagcgtctcg
     2341 ctgatttgtt gctcgacaat aaagctgacc gcttgctgaa agcggatgcg gcaacgttgc
     2401 tggcgctgct gaccagcgat gatgcgccga ccgacgacgt gttaagcgcg gagtttgtgg
     2461 tgcgcaatga acacggcctg catgctcgtc caggtaccat gctggtcaat accattaaac
     2521 aatttaacag tgatattacc gtgacaaacc ttgatggcac cggcaaaccg gcaaacggac
     2581 gtagtctgat gaaagttgtg gcacttggcg ttaagaaagg tcatcgccta cgctttaccg
     2641 cccagggtgc agatgctgaa caggcgctga aagccattgg cgacgctatc gctgctggtc
     2701 ttggggaggg cgcataatga gcagacgtgt tgctacgatc acccttaatc cggcttatga
     2761 ccttgttggt ttctgcccgg aaattgaacg cggcgaagtg aacctggtga aaaccaccgg
     2821 tctgcatgcg gcgggtaaag gcatcaacgt ggccaaagta ttaaaagacc tgggaattga
     2881 tgtcaccgtt ggcggcttcc tgggtaaaga caatcaggat ggttttcagc aactgttcag
     2941 cgagctgggc attgccaacc gtttccaggt tgtacagggg cgcacccgaa ttaacgttaa
     3001 gctgacggaa aaagacggcg aagtgaccga cttcaacttc tcgggtttcg aagtcacccc
     3061 cgccgactgg gaacgctttg tgactgattc tctgagctgg ctcggtcagt tcgatatggt
     3121 ctgtgtcagc ggaagcttac cgtcaggcgt cagcccggaa gcgttcaccg actggatgac
     3181 tcgcctgcgt agtcagtgtc cttgcattat ctttgatagt agccgtgaag cgttagtagc
     3241 aggtttgaaa gcggcaccgt ggctggtgaa acctaaccgc cgcgagttgg aaatctgggc
     3301 aggccgtaaa ctgcctgaaa tgaaagatgt gattgaagct gcacatgcgc tacgtgaaca
     3361 aggtattgcg catgttgtta tttcactggg tgccgaaggc gcgctttggg ttaatgcctc
     3421 cggcgaatgg atcgctaaac caccgtcagt cgatgtcgta agcaccgttg gcgcagggga
     3481 ttctatggtt ggtggcctga tttatggctt gctgatgcgt gaatccagtg aacacacact
     3541 gcgtctggcg acagctgttg cagccctggc ggtaagtcaa agcaatgtgg gtattaccga
     3601 tcgtccgcag ttggccgcaa tgatggcgcg cgtcgactta caacctttta actgacagca
     3661 ggagaggcat aatgaaaacg ctgctgatta ttgacgctaa tctcggtcag gcacgcgcct
     3721 atatggcgaa gaccctgctg ggcgcggcgg cgcgaaaagc aaaactggaa atcatcgaca
     3781 atccgaacga cgcagaaatg gcgattgttc tcggtgattc catcccgaat gacagcgcgc
     3841 tgaacggtaa aaatgtctgg ctgggtgata tttcccgggc agttgcgcac cctgagctgt
     3901 tcctgagtga agccaaaggc catgcgaaac cttacactgc gccggtcact gcgacagcac
     3961 cggttgccgc cagcggtccg aaacgcgtag ttgcggtgac tgcttgcccg actggcgtag
     4021 cacacacctt tatggcggct gaagccattg aaaccgaagc gaaaaaacgt ggctggtggg
     4081 tgaaagttga aacccgtggt tctgttggcg cgggtaatgc aatcactccc gaggaagtag
     4141 ccgcagcgga tctggtgatt gtggcggcag atatcgaagt ggatctggcg aaatttgctg
     4201 gtaaaccgat gtatcgtacc tctaccggtc tggcgctgaa gaaaacctcg caggaactgg
     4261 ataaagcggt tgctgaagca acgccgtatg aaccggcggg caaagctcaa acggcgacca
     4321 ctgaaggtaa gaaagagagt gcaggcgctt atcgtcactt gctaacgggc gtctcttaca
     4381 tgctgccgat ggtcgttgct ggtggtctgt gtatcgcgct ttcttttgct tttggtatcg
     4441 aagcgtttaa agagccgggt acgttggcag cggcgctgat gcagattggt ggtggttcag
     4501 cctttgcgct gatggtgccg gtactggcag gttatattgc cttctccatt gccgatcgtc
     4561 cgggcctcac gccgggtctg attggcggta tgctggcggt cagcaccggt tctggcttca
     4621 ttggcggtat tattgcgggc ttcctggctg gttacattgc gaagttaatc agtacgcaac
     4681 tgaaactgcc gcagagtatg gaggcgctga aaccgatcct gatcattccg ctaatttcca
     4741 gtctggtggt cggtctggcg atgatctacc tgatcggtaa accagttgct ggcattctcg
     4801 aagggctgac tcactggctg cagaccatgg ggactgcgaa tgcggttctg ctgggggcga
     4861 tcctcggtgg catgatgtgt actgacatgg gcggtccggt aaacaaagca gcgtacgcat
     4921 tcggtgtggg tctgctgagt actcaaacct atggcccgat ggcggcgatt atggcggcag
     4981 gtatggtgcc accgctggca atgggcctgg caacaatggt ggcgcgtcgc aaattcgaca
     5041 aagcgcagca ggaaggtggc aaagccgctc tggtattggg actgtgcttc atttcggaag
     5101 gtgcaattcc gtttgctgct cgtgatccga tgcgtgtgct gccgtgctgt atcgtgggtg
     5161 gggcgctgac tggcgcaatc tcaatggcga ttggtgcgaa actgatggca ccgcacggtg
     5221 gtctgtttgt tctgctgatc cctggcgcta ttacgccggt attgggttac ctggtagcaa
     5281 ttattgccgg tacgctggtg gcgggtttgg cctatgcctt cctgaaacgt ccggaagtgg
     5341 acgcagtagc gaaagcagcg taataaaagg tgtgttacag ggcagaaatt tcctgccctg
     5401 taacatttga tgagcgcaaa gagaatgacc agcttgtgaa tgtaatttta tgaatttaaa
     5461 tggttttatc tttcagtaga aacttcaaac gttcatcaaa cactaaggtt atttcctcaa
     5521 gggcagatta ttttctgccc ttttttagtg ttcctgttct gtgcatcccg tcacaaattc
     5581 ctcgcttttg taattcaggc atttaatcgc attttgtgag cctgctcaaa tctgcccatt
     5641 ttgcctctct ggcggtaatt tactttacgt aaaacatgca tactatgagc acatgtttaa
     5701 atatgaacac atgtttaaac cgtctttgca gtatctatat tcttgtggat tgacagggtt
     5761 ggtaattttt taccagagga cgtgctatgc gcgaaaagga ttatgtcgta attataggtt
     5821 cggcgaatat tgatgtcgcc ggatattcac atgaatcatt aaattatgcg gattcaaatc
     5881 caggtaaaat aaaatttacg cctggtggag tagggcgcaa tattgcacaa aacctggcgt
     5941 tgctgggtaa caaagcctgg ctactgagcg ccgtaggcag tgatttttat ggtcaatcgc
     6001 tgctaacgca aaccaatcaa tctggtgttt atgtcgataa atgcctgatt gtgccgggag
     6061 aaaatacgtc gagttattta tcattactcg ataataccgg tgaaatgctg gttgctataa
     6121 atgacatgaa tattagcaac gctattacag ctgaatatct cgcacagcac cgtgaattta
     6181 ttcagagggc aaaggtcatt gtcgcggact gtaatatcag tgaagaggca ctggcatgga
     6241 ttctggataa tgccgccaac gtacccgtat ttgtcgatcc ggtttcagcg tggaaatgtg
     6301 tcaaagtgcg cgaccgtcta aatcagaacc acactctgaa gccaaaccgc cttgaagcgg
     6361 aaaccctgag tgggattgcg ctgtcagggc gtgacgatgt ggcaaaagtt gctgcctggt
     6421 tccatcaaca tggcctgaac cgactggtat tgagcatggg cggcgacggc gtttattaca
     6481 gcgatatcag cggtgaaaat ggctggtctg cgccgatcaa aaccaatgtt attaatgtta
     6541 ccggagcggg cgatgccatg atggcgggac ttgcttcgtg ttgggtagac ggaatgccgt
     6601 ttgccgaatc tgttcgtttc gcacagggat gttcgtcaat ggcgctctcc tgtgaataca
     6661 ccaataaccc cgatttatcg attgccaacg ttatatcgtt agtggagaac gcagaatgtc
     6721 tgaattaaaa atttcccctg aattattaca aatttctccg gaagtgcagg acgctttaaa
     6781 aaacaaaaaa ccggttgtgg cgctggaatc gaccattatt tctcacggga tgccgttccc
     6841 acaaaatgcc cagaccgcaa ttgaagtaga agaaactatt cgtaaacagg gcgcagtacc
     6901 tgccactatc gccattattg gcggcgtgat gaaagtgggt ttaagcaaag aaaaaattga
     6961 attactgggt cgtgaagggc ataacgtgac caaagttagt cgtcgcgatt taccttttgt
     7021 tgttgccgcc ggaaaaaatg gcgcaaccac tgtggcttca acgatgatta ttgcggcgct
     7081 tgccggaatt aaagtatttg ccaccggggg aattggtggt gtgcatcgcg gggcggaaca
     7141 taccttcgat atttctgccg atttgcaaga actggcaaat actaatgtca ccgttgtttg
     7201 tgccggggcg aaatctattc tcgatttagg attaaccact gagtatttag aaaccttcgg
     7261 tgtgccgtta attggctatc agactaaagc gctgcctgcg tttttctgcc gtaccagctc
     7321 gtttgacgtc agcattcgtc tcgacagcgc cagtgaaatt gcccgtgcaa tggcggtgaa
     7381 atggcaaagc gggctgaacg gtggcctcgt ggtagcgaac ccgatcccgg aacagtttgc
     7441 gatgccggag gaatctatca atgcagccat agatcaagcc gtcgccgaag ccgaagagca
     7501 gggcgttatt ggtaaagaaa gtacaccgtt cctgctggcg cgcgttgctg agctgaccag
     7561 cggtgacagc ctgaaatcca atatccagct ggtgttcaac aacgccattc tggcgagtga
     7621 aattgccaaa gaataccagc gtctcgcggg ttaatttctg acgggcagaa agccgtcgcc
     7681 cgtactcatc aagcatacca aaacctggcg ctgacgccgg gtttactggc atgaagggaa
     7741 aatcattatg gatataatga gaagtgttgt ggggatggtg gtgttactgg caatagcatt
     7801 tctgttgtca gtgaataaaa agagcatcag tttgcgcacg gttggagccg cactgctgct
     7861 gcaaatcgct attggtggca tcatgctcta cttcccaccg ggaaaatggg cagtagaaca
     7921 ggcggcatta ggcgttcata aagtgatgtc ttacagtgat gccggtagcg ccttcatttt
     7981 tggttcgctg gttgggccga aaatggatgt cctgtttgac ggtgcgggtt ttatcttcgc
     8041 ctttcgcgta cttccggcga ttattttcgt tactgcgctc atcagtctgc tgtactacat
     8101 tggcgtgatg gggctgctga ttcgcatcct tggcagcatt ttccagaaag ccctcaacat
     8161 cagcaaaatc gaatcttttg ttgcggttac cactattttc ctcgggcaaa atgagatccc
     8221 ggcgatcgtt aaaccgttta tcgatcgcgt gaatcgcaac gagttgttta ccgcaatttg
     8281 tagcgggatg gcgtccattg ctggttcggt gatgattggt tatgccggaa tgggcgtacc
     8341 aattgactac ctgttagcgg catcgctgat ggcgatcccc ggcggtattt tgtttgcacg
     8401 tattcttagc ccggcaaccg agccttcgca ggtcacattt gaaaatctgt cgttcagcga
     8461 aacgccgcca aaaagcttta tcgaagcggc ggcgagcggt gcgatgaccg ggctgaaaat
     8521 cgccgctggt gtagcgacgg tggtaatggc gtttgtcgca attattgcgc tgatcaacgg
     8581 cattatcggc ggaattggcg gctggtttgg tttcgccaat gcctctctgg aaagtatttt
     8641 tggctatgtg ctggcaccgc tggcgtggat catgggtgtg gactggagtg atgccaacct
     8701 tgctggtagc ctgattgggc agaaactggc gattaacgaa ttcgtcgctt acctgagttt
     8761 ctccccatac ctgcaaacgg gcggcacgct ggaagtgaaa accgtggcga ttatatcctt
     8821 tgcgctttgt ggttttgcta actttggttc tatcggtgtt gtcgttggcg cattttcggc
     8881 tatttcgcca aaacgtgctc cagaaatcgc ccagcttggt ttacgggcgc tggcagcagc
     8941 aacgctttcc aacctgatga gtgcgactat tgccgggttc tttattggtc tggcgtaatg
     9001 ctcgatttgt agaccggata aaacgtatta agcgttgcat ccggcaatcg cgctattgca
     9061 aataattttt actgcatcat cccggagaat ttattctccg ggtccatctc cagcgccagt
     9121 cctgacaact gctttctgtt tttaattaga tatcctttct tttaattaat aaaccgtcgt
     9181 gaatgaattg cgcgagaaca tataaaagat gtcgataaga aacgcctaaa tactctgccg
     9241 cttgcgtatg cttttcgtga taaagatcac cttcttgtga gagtaatata aatgctgcca
     9301 ggcgattcac taacggaaat gattgattct gagttaaaga aacaatatta cgataatttt
     9361 tatgacttaa ggtgacgcaa agttttcgta aaaatagggt gtcgtttaat aacagcggac
     9421 ggtaatgttt catagggagc gcaaggcacc aacactcttc aatagcctgc accgcacgcg
     9481 gttcatggtc tttgtcgatt aactcaatct cgccaataaa acagggagcg gcaaagaaat
     9541 cgatcagcga cacgcgacca ttggcaagcg tggcgtaaag cctggcgcgg cctcgcgtca
     9601 gataaaacag ccatgacggc tgttgccctt cctgcacaat gtagtcacgc gctaaaaaat
     9661 gaaacagccg cgtatcggct gaaacatccg ttgaaaagca atccttaaac gcagattcac
     9721 tcatcaattg ctgcttaagg tcattattgt ggatttcttt cacgatgttt atccgctatg
     9781 agatttctca tatttagcat acgtacgctg ttgttaagat tcaatcattg gcccggtcga
     9841 attttctttg ctgtagaaaa aaataacgca actggaaaca gaggaaataa acaatggaaa
     9901 agagaaaaat tattctggat tgtgagccgg gtcatgatga tgctattgca atgatgatgg
     9961 cagcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt aatcagacgc
    10021 ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt aatgttccgg
    10081 tttatgcggg gatgccgcaa cccattatgc gtaaacaaat cgttgccgat aatattcacg
    10141 gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca gaaagtaccc
    10201 atgcggtgaa atatatcatc gataccctga tggcaagcga tggcgatatc actctggtgc
    10261 cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgca atcctgccca
    10321 aaatccgtga aattgtgcta atgggcggcg cttacggtac aggcaacttc accccatctg
    10381 ccgagttcaa tatctttgcc gacccggaag ccgcccgcgt ggtgttcacc tccggcgttc
    10441 cattagtgat gatgggcctc gatcttacca accagaccgt ttgcaccccg gatgtaattg
    10501 ctcggatgga aagggtaggc ggccctgccg gagagctgtt cagcgacatc atgaacttca
    10561 ctctcaaaac gcagttcgaa tactacggcc tggctggcgg cccggtgcat gacgccacct
    10621 gcattggtta tctgatcaac cctgatggca ttaaaaccca ggatatgtac gtcgaagtgg
    10681 acgtcaacag cggcccttgc tacgggcgta cggtctgcga cgaactgggc gttcttggca
    10741 agcccgctaa taccaaagtc ggcatcacta ttgataccga ctggttctgg ggcttagtcg
    10801 aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc cgggacagta
    10861 tcgcaagatg caggcccggc tatttctgat gggcagaaag ccgtcgccca aaatgacatg
    10921 aagagataat cactatggat gtcatgagaa gtgttctggg aatggtggta ctgctggcaa
    10981 ttgcgttttt actgtcagta aacaagaaga agatcagcct gcgtaccgtt ggcgcggcgt
    11041 tagtgttaca ggtcgtgatt ggcgggatta tgctttggtt accgccaggg cgttgggtcg
    11101 cagaaaaagt cgcttttggc gtccacaaag tgatggcgta cagcgacgca ggtagcgcat
    11161 ttatcttcgg ttctctggtc ggaccgaaaa tggatacctt atttgatggc gcaggattta
    11221 tctttggttt cagggtatta ccggcaatta tcttcgtcac tgcactggtg agtattctct
    11281 actacatcgg tgtgatgggg atcttaattc gcattctcgg cggtatcttc cagaaagctt
    11341 taaatatcag caagatcgag tcattcgtcg cggtcaccac cattttcctc gggcaaaacg
    11401 aaattccggc gatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa ttatttacag
    11461 cgatttgtag tggcatggcc tcgattgctg gttcgacaat gattggttac gccgcactgg
    11521 gcgtacctgt ggaatatttg ctggcggcat cgttaatggc gatccctggc gggatcttgt
    11581 ttgcccgcct gttaagcccg gctacggaat cttcgcaggt ttcttttaat aacctctctt
    11641 tcaccgaaac accgccaaaa agcattattg aagccgatgc gacaggggca atgaccgggc
    11701 tgaaaatcgc cgccggtgta gcgacagttg ttatggcatt tgtcgccatc attgcgttaa
    11761 ttaacggtat tatcggcggc gttggcggct ggtttggttt tgaacatgcc tcgctggagt
    11821 ccattttagg ttacctgctg gccccacttg cgtgggtaat gggtgttgac tggagtgatg
    11881 caaatcttgc cgggagtttg attggacaga aactggcaat aaatgaattt gtcgcttatc
    11941 tcaatttctc accctatctg caaacggctg gcactctgga tgctaaaacc gtggcgatta
    12001 tttccttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg gtgggggcgt
    12061 tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca gcttggttta cgcgcgctgg
    12121 cggcggcgac actttctaat ctgatgagtg ccaccattgc cgggttcttt attggtttag
    12181 cgtagtgtgc aatttatgag ccggattaga tgcggtcaag cgtcgaatcc ggcaacattg
    12241 ttacaccatt ggcactaatg acagcgcgtt atcggcagac agggtagggt tgtttaagct
    12301 gccgctggcg cgtgaaattg ccgcgcaggc catcgcaaaa cgggcgctgt cacggaaatt
    12361 gtttccctcc agaaagctat ataccagacc cgccataaaa ccatcgtcag caccaaaact
    12421 gtctaccgtc gtatgcgctg gcacagtcag caaaaattgt tcaccatcct tttcgctgca
    12481 ataaactgac tcatcgggca aataaacaaa cagttgctga acaccttgct gatgcaatgc
    12541 attcactgcg gcattacggt cagcatcgct ggtgatcgcc tgtccccata aaatttccag
    12601 ctccggtaaa gtgggtttca gggtgtgaat atgcgccagc cagtgtttga ttttgcccgc
    12661 tttgaattct gaaacggtat cgacaaacac cgggatctca tcagcgaggg taaagaccca
    12721 ttccagcgcc tcggctgtca ggttgcaatc agccagtacc acgcccgcat gacgaagtaa
    12781 atcgcgcgac ccgttcagta gttgcggtgt caactgttcc agcagatggg tatcgttaat
    12841 tgccagcacg gtttgatcgt ctcgattggc aattgccaga tacgtcgatg tgctttgacc
    12901 atgcaaacga acgcagccgg agacattcac gccggcacgg cgcgtttctt ccaggagcat
    12961 ttcgccataa aagtcatcgc caatcactga aagcaaatgg acgtcacggc ctaacagcgc
    13021 cagattgtgg gcgatattgc gtccaacgcc gcctgccgaa caatgaattg taccgggatg
    13081 agaagccgct tgcgggtaat ggatatccgc catcccgcga atatccatat tgattgtccc
    13141 caccactacg cagtattcct gctcggtgag aatgtaacct ttgtctttaa tccggccttt
    13201 gcgcattaaa tccataatat gcgccgcaac gcgcgaacga ctgatttgca gcatgtccgc
    13261 aatttcgttc tgctgaatca gcgggttacg ccgtaaaatt gcaaggatct ccttttcccg
    13321 gttatttatc ttcaggctac cgctttttca gtttgttgcg ctttcagcca ggcgatctct
    13381 tctgcccaga tatccgggtt gatggtttca aggatcagcg gaatgccgtc gaaacggtcg
    13441 ttctgcataa tccagcggaa cgcatcatga ccgatattgc cttcaccgag gctatgatgg
    13501 cggtcaacgc ggctgccaaa ggtacttttc gcatcgttaa ggtgcatccc gcgcaaatac
    13561 ttaaagccga caatacgggc aaaatcagcg aacgtttttt cgcattcggc tggagtacgc
    13621 aaatcatacc cggcagcgaa agcatggcag gtatcaatgc agacgccgac gcgggattta
    13681 tcttccacgc cgtcgataat cgccgcgaga tgttcgaatt taaaccctaa gttactgccc
    13741 tgaccggcgg tgttttcaat caccgctgtt acaccttgag ttttatccag cgcaatgttg
    13801 atggattcgg caatacgcgc aaggcaatcc tcttctgaaa tctgcatcag atggctgcca
    13861 gggtggaagt tgagcaaaga aagccccagt tgttcgcaac gctgcatttc atctataaag
    13921 gcatcgcgcg atttttccag agcttcagtg accggatgtc cgaggttgat cagataactg
    13981 tcgtggggaa gaatttgcgc cgatgtgtag tgatattttt cacaggcggc tttgaattca
    14041 tcgatggttt gcgtcgtgag cggtgcggca cgccactgac gttggttttt ggtgaacaag
    14101 gcaaacgcgg ttgcgtcgat ttcggcggcg cgaattgcgg catttgccag accgccagca
    14161 gcactaacgt gcgctccaat gtatttcatg cgaggactcc tgttaaaccc gctggaggaa
    14221 aacggtaatg atagcgggtt aacaggagca agaagtagtg gtttatgcga tgacgctttg
    14281 aatcacatag ttaatcgcac caccaccaac aatcagccag gcaaacagta ccagtgccat
    14341 cagcagaggt ttcgccccag cttttttcag cgcgctgacg tgagtggtca gacccagcgc
    14401 cgccatcgcc attgccagca ggaaggtatc cagcgttacc agcatgttca ccacgctctg
    14461 cggtaacagg tggaacgagt taaagatggc aactacgatg aacaagatgg caaaccacgg
    14521 aatagtgatt ttgcttttct cgccgctgtt cgccccagac agctgtttaa cacgcgccgc
    14581 cagcaggatg aggaacggag ccagcatcat cacgcgcagc attttggaaa taactgctgc
    14641 gttttctgca tccgggctga tggcatgacc tgccgccacc acctgcgcca cttcgtgcac
    14701 agtagaacca atgtagatac cgaaagtttc cggactaaac cattgagaca tcagcggata
    14761 tatcgccggg tagaggaaaa tcgcgacggt cccgaagata acaacggttg caacagccac
    14821 ggttacttta ctggcttccg ctttcactac cggctcagtc gccagtaccg cggcagcacc
    14881 acagatactg ctaccggcac cgatcaacca actggtgtgc ttatccagac caaacacttt
    14941 ctgccccagg aagcaagcca gcaggaaggt actggacagc gtcagcacgt caatgatgat
    15001 cccactgata ccgacatcgg caatttgcga gaacgtcaga cggaagccat aaagaatgat
    15061 acccagacgt aataaatatt gcttggcaaa cagcacacca ccgtcacagc ttttccagat
    15121 gtgcggatag atggtgttgc ctaaaaccat ccccaacaag attgcgaggg tgagggcact
    15181 aaacccggca cccgcaaccg cgggaatgga accaccccac agggcgaccc cggtgataac
    15241 tgcactcagg gctaaccccg gaataaaatg ccacagtgta cgatgttgtt tctgtaaggt
    15301 gatattcgtc ataaccctct cctttaaccg ggcataaggt tacgactaac tggtttaaaa
    15361 ataaaattga ttatatattt ataattaatc tttataagtg gtaagcgact atgcacatca
    15421 ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc acccaggcgt
    15481 cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgactgac ctggaagggc
    15541 agcttggcgt tcaactgttt gatcgcgtgg ggaaaagact ggttgttaat gaacacgggc
    15601 ggctgctcta tccgcgagcg ttggcactgc ttgaacaggc ggttgaaatc gaacaactgt
    15661 ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt aactacattc
    15721 tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt gaacttagcg
    15781 ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat attggcttta
    15841 ttgaaggacc gtgccacagc actgaaatca tttctgaacc gtggctggaa gacgagctgg
    15901 tggttttcgc cgcgccgact tcgccgttgg ctcgtggtcc ggtcacctta gaacagctgg
    15961 ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggatatt gtcgattatc
    16021 tgttgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac tccgaggcaa
    16081 tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgt gtgattgaag
    16141 atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc ctgatgcgta
    16201 cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg cgctttctgg
    16261 actattgcga tcccgcaaat gtgccgcgtt aagttgctgt acaagaacat gctggtgctg
    16321 tgtcgatttc gtgacgcagc gccttcagca tgcattcgcc agaaaagaga ttggctgctt
    16381 tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc tgccggaagg
    16441 attgccaatc gtctgctaca atcgcgccat catttttaag atggatagca tttttgtatg
    16501 ggttccgaaa ctaaaactac agaagcgccg ggcttacgcc gtgaattaaa ggcgcgtcac
    16561 ctgacgatga ttgccattgg cggttccatc ggtacaggtc tttttgttgc ctctggcgca
    16621 acgatttctc aggcaggtcc gggcggggca ttgctctcgt atatgctgat tggcctgatg
    16681 gtttacttcc tgatgaccag tctcggtgaa ttggctgcat atatgccggt ttccggttcg
    16741 tttgccactt acggtcagaa ctatgttgaa gaaggctttg gcttcgcgct gggctggaac
    16801 tactggtaca actgggcggt gactattgcc gttgacctgg ttgcagcaca gctggtcatg
    16861 agctggtggt tcccggatac accgggctgg atctggagtg cgttgttcct cggcgttatc
    16921 ttcctgttga actacatctc tgttcgcggc tttggtgaag cggaatactg gttctcactg
    16981 atcaaagtca cgacagttat tgtctttatc atcgttggtg tgctgatgat tatcggtatc
    17041 ttcaaaggcg cgcagcctgc gggctggagc aactggacaa tcggcgaagc gccgtttgct
    17101 ggtggttttg cggcgatgat cggcgtagct atgattgtcg gcttctcttt ccagggaacc
    17161 gagctgatcg gtattgctgc aggcgagtcc gaagatccgg cgaaaaacat tccacgcgcg
    17221 gtacgtcagg tattctggcg aatcctgttg ttctatgtgt tcgcgatcct gattatcagc
    17281 ctgattattc cgtataccga tccgagcctg ctgcgtaacg atgttaaaga catcagcgtc
    17341 agtccgttca ccctggtgtt ccagcacgcg ggtctgctct ctgcggcggc ggtgatgaac
    17401 gcagttattc tgacggcggt gctgtcagca ggtaactccg gtatgtatgc gtctactcgt
    17461 atgctgtaca ccctggcgtg tgacggtaaa gcgccgcgca ttttcgctaa attgtcgcgt
    17521 ggtggcgtgc cgcgtaatgc gctgtatgcg acgacggtga ttgccggttt gtgcttcctg
    17581 acctccatgt ttggcaacca gacggtatac ctgtggctgc tgaacacctc cgggatgacg
    17641 ggttttatcg cctggctggg gattgccatt agccactatc gcttccgtcg cggttacgta
    17701 ttgcagggac acgacattaa cgatctgccg taccgttcag gtttcttccc actggggccg
    17761 atcttcgcat tcattctgtg tctgattatc actttgggcc agaactacga agcgttcctg
    17821 aaagatacca ttgactgggg cggcgtagcg gcaacgtata ttggtatccc gctgttcctg
    17881 attatttggt tcggctacaa gctgattaaa ggaactcact tcgtacgcta cagcgaaatg
    17941 aagttcccgc agaacgataa gaaataagtt tcctcccttc cttgctaagc cctctcaacc
    18001 gagagggctt tttcaattcc atttccctga caaatcatgc ggatataaaa tttaacattt
    18061 ggattgataa ttgttatcgt ttgcattatc gttacgacgc aatcaaaaaa ggctgacaaa
    18121 tcagaggctg ttccggcttt ctgggatgat cacctgcata caaataagtc caccgcgatg
    18181 ctgccgtacg caaggggacg taaagaagat gtgagcgata cccattttat tttcgtagtt
    18241 acctcatgga gatatggaat gtttaggttg aaccctttcg tacgggtcgg gctgtgtttg
    18301 tccgctattt cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga aacgatggtt
    18361 gtcactgcat cttccgtgga acaaaatctt aaagatgcac ccgccagtat cagcgtcatt
    18421 acccaggaag acctgcagcg aaaaccggta cagaatctga aggatgtcct caaagaagtg
    18481 cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat tcgtggtctg
    18541 gacagcagct acaccctgat tcttgtcgac ggtaaacgcg ttaactcccg caatgccgtc
    18601 ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga acgtattgaa
    18661 gtggttcgtg gcccgatgtc gtcgctatac ggctccgatg cgctcggcgg tgtagtgaat
    18721 atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga taccaccatt
    18781 caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag tggaccatta
    18841 attgatggtg tgctgggaat gaaagcttac ggcagcctgg caaaacgtga aaaggatgac
    18901 ccgcaaaact caacgaccac cgataccgga gaaacgccgc gtattgaagg attctccagc
    18961 cgcgacggca atgtcgaatt tgcctagaca ccgaatcaaa atcacgattt tactgccgga
    19021 tacggtttcg accgtcagga tcgtgattcc gattcgctgg acaaaaaccg cctggaacgc
    19081 cagaactact gcgtcagcca taatgggcgt tgggattacg gcaccagcga actgaaatcc
    19141 tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac ttccgaaagc
    19201 aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt tctcacggtt
    19261 ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg gggaaccagc
    19321 tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg gatcttcgag
    19381 ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg tgaacactgg
    19441 agtccgcgtg cctacatggt ttataacgcc accgacaccg taacggtgaa agggggctgg
    19501 gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac gagcaattcc
    19561 tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac cagcgaaagt
    19621 tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggg tgaatccagc
    19681 gttatcgttt tccgtaacga tgtgaaagat cgtatcagca tcagccgtac gtctgacgtc
    19741 aacgctgcac cgggctacca aaactttgtt ggttttgaga cgggcgctaa cggacggcgc
    19801 ataccggtat ttagctacta cagcgttaac aaagctcgta ttcagggcgt ggaaaccgaa
    19861 ctgaaaattc cgttcaacga tgaatggaaa ctgtcgatca actacaccta caacgatggt
    19921 cgtgatgtca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca tactgctaac
    19981 ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac
    20041 tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg ttataccatc
    20101 tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg
    20161 aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga cggacgtcgt
    20221 tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact
    20281 ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga
    20341 agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc
    20401 gcgcggcttc tgccaatacc gcaggttgca actggtcggc aagaaactga tcattatcgc
    20461 cctgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc agcgcgcaac
    20521 tatcccattc cagcatgcat ttttgtcctc acctaaatag gtgctaaacg ctttgattcc
    20581 ccacgggacg ctgcacggat tcacaatggg cgcaaaggcc gaaacgctgg tgtatttacc
    20641 cggatttttc agcgccataa tcagcgcacc gtgaccaccc attgagtgac cgctaatggc
    20701 gcagcggtcg ctgacattaa attgcgactg aaccagcgcc ggtaattcat cgcgcagata
    20761 atcatacatc cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat aaaagcctgc
    20821 gccctggcct aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg ggctggtgtc
    20881 tggcatcacc agtacaatcc ccagttccgc cgctacccgc tgggcacccg ccttggtggt
    20941 gaagttctcg tcattgcagg ttaatccgga aagccagtac agcactggtg gcggggtgtg
    21001 atcacgaggg ggggggagaa agatactgaa cgtcatcggg cagtttaagg tactggagtc
    21061 gtgtcgccag cgttgctgcc agccttcaaa acagcggtgc tcttcgagca tttccatgcg
    21121 tggctccttg ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa tgtgctgtga
    21181 gtaactttca cttccgtatt tgcataacga tgttttaaca tctgctgatg aaaggcagcg
    21241 gcaattacaa taattatcgc tgtgaatact ggattatgtg cgccgcctca cgcacaataa
    21301 tcaagctgta aatcagctta ataactttgc ccccacgcag ggtggaggcg tcacacctgc
    21361 aggagaaatc ataaatgcca tcactcagta aagaagcggc cctggttcat gaagcgttag
    21421 ttgcgagagg actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat aacgaaacgc
    21481 gcaaaagcct tattgctggt catatgaccg aaatcatgca gctgctgaat ctcgacctgg
    21541 ctgatgacag tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc gatgaaattt
    21601 tctccggtct ggattacgcc aatttcccga aaatcaccct cattgaaaac aaaatgaagg
    21661 tcgatgaaat ggtcaccgtg cgcgatatca ctctgaccag tacctgtgaa caccattttg
    21721 ttaccatcga tggcaaagcg acggtggctt atatcccgaa agattcggtg atcggtctgt
    21781 caaaaattaa ccgtatcgtg cagttctttg cccagcgtcc gcaggtgcag gaacgtctga
    21841 cgcagcaaat tcttattgcg ctacaaacgc tgctgggcac caataacgtg gctgtctcga
    21901 tcgacgcggt gcattactgc gtgaaggcgc gtggcatccg cgatgcaacc agtgccacga
    21961 caacgacctc tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac gagtttctgc
    22021 gcgctgtgcg ccatcacaac tgattaaaag gcaggaacca tggagcgcaa cgtcacgctc
    22081 gattttgttc gcggcgtcgc cattctgggg atcctgctat taaacatcag cgcctttggg
    22141 ctaccaaagg cggcttatct caatcccgcc tggtacgatg ctattacgcc gcaggatgca
    22201 tggacctggg catttctcga tctcatcggc caggtgaaat tcctcacgct ttttgcgctg
    22261 ctgtttggtg ctggcctgca aatgttgctg ccccgtggca gacgctggat ccagtcgcgg
    22321 ttaacgctgt tagtcttgtt gggctttatt cacggtttat tgttctggga cggcgatatt
    22381 ctgctggctt acgggctggt ggggcttggc gttttgctgt tattggggct gatttccgac
    22441 agccagacca gccgcgcctg gacgccggat gcatcggcta ttttgtatga aaaatactgg
    22501 aagcttcacg gcggcgttga agcgatcagt aatcgtgccg atggtgttgg caacagttta
    22561 ctggcactgg gcgcacagta tggctggcaa ctggcaggga tgatgctcat tggtgcggcg
    22621 ctgatgcgca gcggctggct gaaagggcag ttcagcttac gtcactatcg tcgtactggt
    22681 tttgtgctgg tggcgattgg ggtgaccatt aaccttcctg ccatcgccct gcaatggcag
    22741 ctggactggg cgtatcgctg gtgtgccttc ttacttcaaa tgccgcggga actgagtgcg
    22801 ccgtttcagg cgattggcta tgcgtcgctg ttttatggct tctggccgca attgagccgc
    22861 tttaagctgg tgcttgcgat cgcctgcgtc ggacggatgg cgctgaccaa ctatctattg
    22921 caaacgctga tttgtaccac gcttttttac cacctcggtt tgtttatgca ttttgaccgc
    22981 ctggagctgc tggcgtttgt tattccggta tggctggcga atatcctctt ctctgttatc
    23041 tggctgcgtt acttccgcca ggggccggtg gaatggctct ggcgtcagtt aactttgcgt
    23101 gctgccggac cggcaatatc taaaacatca agataacgat ctggattaca ttcgttaaca
    23161 aaacggctgt aaccgtttcc attgctgtga ctcgattcac gaagtcctgt attcagtgct
    23221 gacaaaatag ccgccagcaa gcagtcattt actgcaatct cataacaggt agtgaatatg
    23281 atcaccattc gtgatgtagc gcgtcaggct ggcgtctctg tggcaacggt ttcccgggtg
    23341 ctcaataaca gcacgctggt cagtgccgac acgcgtgaag cagtaatgaa agccgtgagt
    23401 gagctggatt atcggccaaa cgccaatgcc caggcgctgg caactcaggt tagcgacacc
    23461 attggcgtgg tggtgatgga cgtttctgat gcgtttttcg gcgcgctggt aaaagcggtg
    23521 gatctggtcg cccagcagca tcagaaatac gtgctaatcg gcaatagcta tcatgaagcg
    23581 gaaaaagagc gtcacgccat tgaggtgtta attcgccagc gttgtaatgc gttgattgtt
    23641 cactcaaaag cattgagtga cgatgaactg gcgcaattta tggataacat tcccggtatg
    23701 gtgttaatca accgcgttgt gccggggtac gcccatcgtt gcgtttgcct ggataatctc
    23761 agcggtgccc gaatggcgac gcgcatgttg ctgaataacg gtcatcaacg tattggttat
    23821 ctttcttcca gccacggcat tgaagatgac gccatgcgta aagcaggctg gatgagtgcg
    23881 ttgaaagagc aggatattat tccgccggaa agctggattg gcactggtac gccggacatg
    23941 ccgggcggtg aggcggcgat ggttgaactg ctggggcgca atctacaact taccgctgta
    24001 tttgcttata acgacaatat ggccgctggc gcactgacag cattaaaaga taatggcatt
    24061 gcgattccgt tacatctctc aatcatcggt ttcgatgata ttcccatcgc ccgttacacc
    24121 gacccgcaat taacgaccgt gcgttatccc attgcttcaa tggctaaatt agccaccgaa
    24181 ctggccttgc agggggcagc aggcaatatt gatcctcgtg ccagccactg ttttatgccg
    24241 acgttagtgc gtcgccattc tgtcgcaacg cgccagaatg cggcggcgat cactaactca
    24301 acaaatcagg cgatgtaacc gctttcaatc tgtgagtgat ttcacagtat cttaacaatg
    24361 tgatagctat gattgcaccg ttttaatgtt gtaacccgta tgtaacagtg aataatcact
    24421 ttcaccgagg taatagcgtc ataacaacaa ttaaagccgt tttctggagc gttaccgggc
    24481 atggaagaac gaattttaaa aagtgagctt cggcgttcag taacacttca ttaactctac
    24541 tgccccgccg agcatttatc tcaagcacta ccctgcataa gaaaaaccgg agataccatg
    24601 aataagaagg tgttaaccct gtctgctgtg atggccagca tgttattcgg tgccgctgca
    24661 cacgctgctg atactcgcat tggtgtaaca atctataagt acgacgataa ctttatgtct
    24721 gtagtgcgca aggctattga gcaagatgcg aaagccgcgc cagatgttca gctgctgatg
    24781 aatgattctc agaatgacca gtccaagcag aacgatcaga tcgacgtatt gctggcgaaa
    24841 ggggtgaagg cactggcaat caacctggtt gacccggcag ctgcgggtac ggtgattgag
    24901 aaagcgcgtg ggcaaaacgt gccggtggtt ttcttcaaca aagaaccgtc tcgtaaggcg
    24961 ctggatagct acgacaaagc ctactacgtt ggcactgact ccaaagagtc cggcattatt
    25021 cagggcgatt tgattgctaa acactgggcg gcgaatcagg gttgggatct gaacaaagac
    25081 ggtcagattc agttcgtact gctgaaaggt gaaccgggcc atccggatgc agaagcacgt
    25141 accacttacg tgattaaaga attgaacgat aaaggcatca aaactgaaca gttacagtta
    25201 gataccgcaa tgtgggacac cgctcaggcg aaagataaga tggacgcctg gctgtctggc
    25261 ccgaacgcca acaaaatcga agtggttatc gccaacaacg atgcgatggc aatgggcgcg
    25321 gtagaagcac tgaaagcaca caacaagtcc agcattccgg tgtttggcgt cgatgcgctg
    25381 ccagaagcgc tggcgctggt gaaatccggt gcactggcgg gcacagtact gaacgatgct
    25441 aacaaccagg cgaaagcgac ctttgatctg gcgaaaaacc tggccgatgg caaaggtgcg
    25501 gctgatggca ccaactggaa aatcgacaac aaagtggtcc gcgtacctta tgttggcgta
    25561 gataaagaca acttggctga attcagcaag aaataagact gattgggcgc agtcgtttac
    25621 tgcgccctta ttgtatggcc aacaaggtaa aattatatgg tcagctcaac gactccgtct
    25681 tccggggaat acttgttgga aatgagcggt atcaacaagt cctttcctgg tgttaaggca
    25741 cttgataacg ttaatttaaa agtccggcca cattctatcc atgcattaat gggggaaaac
    25801 ggtgcaggaa aatcgacatt attaaaatgc ctgtttggta tttatcaaaa agactccggc
    25861 accattttat tccagggtaa agagatcgat ttccattccg ccaaagaggc actggaaaat
    25921 ggtatttcga tggtacacca ggagttaaac ctggtattac aacgttcggt gatggataac
    25981 atgtggctgg ggcgatatcc caccaaaggc atgtttgtcg atcaggacaa aatgtaccgc
    26041 gaaaccaaag cgatttttga tgaactggat attgatatcg atccgcgtgc gcgcgtcggc
    26101 acattatccg tttcgcaaat gcagatgatc gaaatcgcca aagcgttttc ctataacgcg
    26161 aaaattgtga ttatggatga accgacttct tcgttaaccg aaaaagaggt caatcatctg
    26221 ttcactatta ttcgtaaatt aaaagagcgc ggctgcggta ttgtttatat ctcgcataaa
    26281 atggaagaaa tcttccagtt atgtgatgaa gttaccgtat tgcgcgacgg tcagtggatc
    26341 gccaccgaac cgctggcagg actgacgatg gacaagatca tcgccatgat ggttgggcgt
    26401 tctcttaatc agcgttttcc tgacaaagaa aacaagccgg gcgaagtcat cctcgaggtg
    26461 cgtaacctaa cgtcactgcg ccagccgtcg attcgcgatg tctcgtttga tctgcataaa
    26521 ggggagatcc tcggtattgc cgggctggtg ggggcgaaac gtaccgatat tgttgagacg
    26581 ttatttggta ttcgcgagaa atcggctggc accattacgt tgcacggcaa acagatcaat
    26641 aaccataatg ccaacgaagc cataaaccac ggatttgcac tggtaactga ggagcgccgc
    26701 tcaacgggaa tttatgccta tctggatatt ggttttaact cgttaatttc caatattcgc
    26761 aactacaaaa ataaagttgg tttactggat aactcgcgga tgaaaagcga tacccagtgg
    26821 gtgattgatt cgatgcgggt aaaaacgccg ggtcatcgga cgcaaattgg ttcgctctcc
    26881 ggtggtaatc agcaaaaggt gattattggt cgctggctac taacgcaacc agaaatatta
    26941 atgctcgatg aaccgacgcg cggtattgat gtcggggcga agtttgaaat ttatcagtta
    27001 attgccgaac tggcgaagaa aggcaagggg attattatta tctcctctga aatgcctgag
    27061 ttgttaggga taacagaccg tattctggtc atgagcaatg gtctcgtttc cggaattgtc
    27121 gatacaaaaa caacaacgca aaacgaaatt ctgcgtcttg cgtctttgca cctttaagat
    27181 taggggcttc ccatgagtgc gttaaataag aaaagttttc ttacttacct gaaagagggc
    27241 ggtatttacg tcgttctttt agttttgctg gcgattatta ttttccagga cccaacattt
    27301 ttaagtctgt tgaacttaag taatattctc acccagtcat cggtgcgtat tattatcgcg
    27361 ctcggtgtgg cagggttaat tgtcacccag gggaccgatc tttctgctgg tcgtcaggta
    27421 gggctggcgg cagtggtggc tgcgacatta ttgcagtcca tggataacgc caacaaagtg
    27481 ttcccggaaa tggcgacgat gccgattgcg ctggttattc tgattgtctg tgccattggt
    27541 gcggtgatcg gtttgatcaa cggtctgatt atcgcttatc tcaacgtgac gccgttcatt
    27601 accacgctcg gcacgatgat catcgtctat ggcatcaact cgctctatta cgactttgtc
    27661 ggggcgtcgc caatttctgg ttttgacagt ggcttctcta cctttgctca gggctttgtc
    27721 gcgctgggga gtttccgtct ctcttacatc accttctacg cgttgattgc ggtggcgttc
    27781 gtctgggtgt tgtggaacaa aacccgcttc ggtaagaaca tttttgccat tggcggtaac
    27841 ccggaagcgg caaaaatatc tggtgtcaac gtcggcctga acctgctgat gatctacgcg
    27901 ttgtctggcg tgttctatgc ctttggcggg atgttagaag ccggacgtat cggctctgcc
    27961 accaacaacc tcggctttat gtatgagctg gatgctatcg cggcgtgcgt ggtaggcggt
    28021 gtatcgttca gcggcggtgt ggggacggtg attggcgtgg tgaccggggt aattattttt
    28081 accgtcatca actatggcct gacgtatatc ggcgtaaacc catactggca gtacatcatc
    28141 aaaggggcga ttattatctt cgccgtagcg ctggattcac tgaaatacgc gcgtaagaag
    28201 tgatgtgatt gcggatattg ccggatgcgt ctctggtttt acaacgttac cgggtgttct
    28261 ttctcgcctt tcttaaactt cacttccccg agctcaatac aacccaccgg gcagacgtga
    28321 ccacaaagca gacaacccac acatttctcg gtattacaat gcggtgtgcg ggttttctcg
    28381 ctccattcca tcgcctggtg accgccgtcg taacaggaaa tataacagcg tccacagcca
    28441 acacatttgt caagattgat acggggatag acaatataac tgcggtctaa atcttccgcc
    28501 gggacaatat tgttattcgc cagacctacc atctcctgca acgaatcaaa tccctgatcg
    28561 gcgagataat gcgataaccc gctcgccata tcttctacga tgcgatagcc gtactgcata
    28621 atgccggtgg tgacctgtaa cgttgctgcg ccgagcaata aaaactccgc cgcatcttcc
    28681 caggtttcaa tgccgccgat accgctgatt gggaaatcgc gcagttctgg atgggtgcgc
    28741 atttgctgaa tgaagcgcag ggcgatcggt tttaccgctt taccggaata tccggaaata
    28801 ctcgatttac cgttaacgat cggcataccg attttctgat taagatcgat attggtgatg
    28861 gatttaacgg tgttaattgc cgcaatgcca tctgcgccgc cgcgcttcgc cgccagcgcc
    28921 acttcgcaca tatcaccgat attcggcgtc atcttcgcca gcattggcag cgtggaaccc
    28981 cgtttcactg cccgacaata tttttctacc agctccgggc tttgcccgac atcgctaccc
    29041 atcgcatgag aagtcatttg cggacaggag aagttacact cgatcatatc cgcgccagct
    29101 gctcccattg ctgctcattt tcccccatga tcgaagcgat caatactttg tccgggtaat
    29161 cttccttcag ccgacgcagg gcggtcagat tctcttccaa cggatgttca gcaatctgct
    29221 ccatattttt gaagccgata aaaccggtat cttctttcac cagatgatca aaacgcggcg
    29281 agacttcgtt ggcgataaaa aagccgatcg ttttaaacac cacaccgccc caacctgtgt
    29341 cgtaggcttt ggcacacatc tcatagcagt tgcctaccgg cgaagaagag aggcagaacg
    29401 ggttgggaaa gttcacgccg caaaaagtaa tcgaaagatc tttcgttaac atgagcaagc
    29461 tccctctaaa tagtgatgaa tcgcctcggc ggcttctttc ccggttttca cggcatagac
    29521 cacggttttg tcaccctcaa caatatcgcc agcagcaaag acttgcggat cgcggatctg
    29581 ataattttgt gttttgatgg tgttacgctg ctgctctaac tccgcaaagg catccagtct
    29641 ggcatgctga ccgacggcga gaataatttt atctgccgcc atcgtcagtt cgcctgataa
    29701 ccgtacatgc ttaaacgtga ctttattgcc ttccacggct actggcgtga atccatcgat
    29761 gatcgaaacg cccagctccc gggcgctggc aaactctttt tcgcttgccg gaaactcatc
    29821 taactcttca cgtgctacgc aagttaccgc ctgacagccg agaactttca gcgtgctggc
    29881 tacgtccatc gcgacatcac cgccgccgat aattaatgcg ctttgtggaa tgctgatatc
    29941 gccttgcgcc tgtcgtacac gttgcaggaa gtcgacggca atctcaacgt cgctatgctc
    30001 aaacagcggt agcccggaac cgcttgataa ccccacggtg accagtaccg cgcggttttc
    30061 cgctttcagt tgctccaggg tgagtgtttt accgatttcg ttattgcact taatggtcac
    30121 gcccattttt tcaatacggg cgatctctgc atccagcact gattgcggta accggaattg
    30181 cggaataccg ttacgcaacc agccaccggg gtgcgcttct ttctcataaa tcgtgacgtc
    30241 ataaccctgg tttgtcagtg tcacactggc ctgcaatccg gcaggacctg cgccaataat
    30301 cgcgactttg ccgagcgttt tagtaccggg ctgataaatt tccatcccgg tttgttgttc
    30361 aaaatcagta acaaaacgct gtaagcggcc gatatcaatg ggagcatcaa caccggcacg
    30421 ggtacaaccg ctttggcata atttctccgt cgggcacact ctggcacaaa cggcaccgag
    30481 ggcattattt tcgcgaattg tctcggcagc gcctttaaaa ttacgaaagt agattgagcg
    30541 aataaatttc cccggatcgg tctgtgccgg gcaagcctga ctacagggag cgtcgtgaca
    30601 taataaacag cgagaggctt ctttaatcgc cagtaacggc gtaaaagccg gagtgagttc
    30661 atccagataa ttttgttgcg gcatgtgatc ttccttaatg ctatgagtaa ttatggcgaa
    30721 cgctaaataa atattgcgtg ctgatattaa ggctatgcgc gtggggaaat ttaagctgtg
    30781 aaaggattca caaatagctg cggttcagaa tgttatatcc gcagcttatt gagtgaagta
    30841 ataattattc tcaataatta acagaaaaga gaaaatatta acgatagaaa ataagataaa
    30901 caatcatgcc gatagataaa attccgaagc caatatatgc caccatataa gcgaagaaga
    30961 gtccgggatt ttttgctcgc cagacataca ccggttccgg tgcattttta acccgtttat
    31021 caatcgcgcc tgcgcgccag cctttccatg cttcgcggaa acaaaatatc gggataagga
    31081 aaaaaacggc aacgacaaaa aactgctgta catccataac tactttcctt gtttcttttg
    31141 taattcaagt aactgttcgg gtgtaacggc gggatacccc tgcgcatctt ccggcacctg
    31201 gtgcatagcc ggaataggga ccagcggccc taaaaaacgc ggttcacgtt taaaaatata
    31261 aaggtcagcc agcgcaccga aacgggcggc aaattcgcga atacgtactg acagcatatc
    31321 tttcggtgac ggtacggcat aacactgagc ctgaatcccc atatgcagcg caataaataa
    31381 tgctcgctca cagtggaaac gttgggtgat aataatgaaa tcattagtat cgaaaacttt
    31441 gcgtgtacgc acgatggaat ccagcgtacg aaagcctgcg taatcgagaa caatatctga
    31501 tgggtcgaca ccagcagcga ttaaatcttt gcgcatggtc atcggctcgt tataactttg
    31561 caatgcgtta tcgccgctca gtaatagata atttacctta ccgctgttat aggcattaat
    31621 cgctccttga atgcggtagc gataatactg attaataacg ccggtacgat aatattttgc
    31681 tgtgccgagc accacgccga cctggcggta ggggagatcc tgcaattcgt cgtagatata
    31741 aggcgcggtt ttccagctca tccagcgatc gaggccgagc acagtcaaca gcagcaagcc
    31801 gatcaggact aacaggctga ggaacacgcg ctttaacatg caggagactc gagctaagga
    31861 aaatttccat caggctactt tacccgctgg ttttgcgcaa gaaacggcaa actgtttgcc
    31921 gggaatttcg acaccaggag agggctggca agccggacaa ggcgttcacg ccgcatccgg
    31981 caccaggctt aagcgagaag cacgcggtcg atactgtgac agccgagagc tttcagcgtt
    32041 gcggaggtgg catcccactg aatcaacggc gcatcggctt tttctgccag aaccgcgcgc
    32101 tggatatccg ggtaatcata acccttgaga ttcaacagaa ttaacgcccc ctgcaacggc
    32161 ggcagagtcg ggttgaatgc ggcgttttca gcgtagctgc cgctgaaaat acgaccgtct
    32221 ttacattcca gcgcgacacc gcttggtgac ttactgtaag gcatgtgcga acggtttgcc
    32281 gctgcaatcg ccgcctgaga aagcgcatca ccagtcagcg catagccgtg atcctgctcg
    32341 tccatcagca gcgttttaat ctccagatct ttcggcccaa aggcatccgg cagatagtca
    32401 cgcagcgcgt gtgcctcgcg gcccggcaaa tgaatacgca gatccagacc gctgttcagt
    32461 tcattcataa actgacggca gtgaccacaa ggcgtatagt taacggtgat ggctgcaagc
    32521 gctttttcac cactcaacca agcgtggctg atcgcgcttt gttcggcatg aacggtttgc
    32581 tgcattgttg caccaataaa ttccatattg gcaccaaaat accaggttcc gctcacaccg
    32641 cgcgcaattg cgccaacatt aaaattcgac aatggcgtac gcgcacaggc cgccgccagc
    32701 ggaagtagtg cgaatgccag cgcgtcttcg tccagtcccg ttgcgctctt cagcgatgag
    32761 acttgctccc cggtcaacaa agcggggaag tacttgtctg ccagaatagg ttccagtgca
    32821 gattgcaagt tatccgcaag ttgggcaaaa gcggtttgaa aacgtggatg catggcgttg
    32881 cctcataatc atgtaatggg ataacagttt acgaacccgt tatggcttta tatgtgatct
    32941 gaatctcatt aattatgcaa cacgtgaaac ttacatcaaa aatgcgcgac gtatcgcaaa
    33001 ttttagccca ttactgccag aataatcggg aaaaggaacg gtgcgatcag cgaagtaatt
    33061 atcccgcata acaccagcgc tagcgaacta aatgcacctt cctgataatc cagctcggcg
    33121 cagcgcgccg taccgagggc gtgcgaggca gtccccattg ccagaccgcg cgcagctttg
    33181 gtacgaatac gcatcgcatt aagcaatgta tggccaaata ccgcgccgag gatgccgacg
    33241 aaaatcacgc aaactgcgct gattgccgga ataccgccaa tactgccgcc aaccgccatt
    33301 gcaataggcg tggtgactga tttcggcagg attgacgcgg cgatttccgg tgaagcgccc
    33361 atcaacaatg ccacggaagt ccccgtcacc atcgcaacca cgctgccgat aaaacagatg
    33421 gtgatgatcg atttccagcg cgcgcggatc tggtgtagct gctcatataa aggataggcc
    33481 agcgcgacga ccgccggttg cagcagatcg tttagcactt cgctaccttt aaagtaactg
    33541 tcgtaagaga tgccggtcag cattaaaaaa ggaatgatca ccaccattgc taccagcaac
    33601 gggttaagca ggggaaactt ataccgtgcc gccagtttgc gggcggcaaa aaagacaatc
    33661 aacgtcagcg gtaatgacca ccagatattc gccatcattc ttctgatcct ttctgaccta
    33721 cgactttacg ttcaccgtgt accagttgtg aactccagct caccaccaga aaaaccacca
    33781 gcgtactgac agcacaggaa accactaccg ggccaaactg cgcgcggagc aaatcaaaat
    33841 attgcatgac gcctacgcca atcggcacaa acaatagcgc catatagcga atcagtacgt
    33901 agcaccccgg attgacccat ttcgccggaa gaatttgcaa ggccagcagg acaaacagga
    33961 tcagcatccc gatgatgctg cccggaatgg ttaccggtag cagggaagca atgaaaatgc
    34021 ctgcatacag gcaggcataa atcaggacga aagcgcgtaa atattgccag ataatgttca
    34081 gtgtcttact cataatcaac accccagatg agatggattc atcatacaat taattcgtaa
    34141 aacgtgctac cgatcacatt aagaaatcag cgcttatttt ttcgctaccc ggtattacgc
    34201 aatgagcagc gaactcaggc atacatcaag catagagagg gattgcagtt tgcagcaatc
    34261 cctctttgca gacctttaat ccgctgacca taggcgctaa catagcgcct catttttttg
    34321 cgggtgatga tatgcgtgtg ttactggcac cgatggaggg agtgcttgac tctctggtgc
    34381 gtgaattgct gaccgaagtt aacgactacg atctgtgcat caccgagttt gtccgcgtgg
    34441 tggatcaact gctgccggta aaagtctttc atcgcatttg ccctgagcta caaaacgcca
    34501 gccggacacc atctggtacg ctggtgcgcg tgcagttgtt aggtcagttc ccacaatggc
    34561 tggcagagaa cgccgcccgt gcggtggagt taggtttctg gggcgtggat ctcaattgcg
    34621 gctgcccgtc gaaaacggtt aacggtagcg gcggcggggc gacgttactc aaagatcctg
    34681 aactcatcta ccagggggca aaagcgatgc gtgaagctgt accggcgcat ttgcctgtca
    34741 gcgtgaaagt gcgtctgggc tgggacagcg gtgagaagaa atttgaaatc gccgatgcgg
    34801 ttcaacaggc tggcgctacg gagctggtgg tgcatgggcg gacgaaagag cagggttacc
    34861 gcgcggagca tattgactgg caggcgattg gcgagattcg ccagcggctg aatattccgg
    34921 tgattgccaa cggtgaaatc tgggactggc agagcgcgca acaatgcatg gcgatcagcg
    34981 gctgcgacgc agtgatgatt ggtcgcgggg cgctcaatat tcccaacctg agccgggtgg
    35041 taaaatataa cgaaccgcga atgccgtggc cggaggtggt tgctttgctg caaaaatata
    35101 cccgtctgga aaagcagggc gataccgggt tatatcacgt tgcgcggatt aaacagtggt
    35161 tgagttattt gcgtaaagaa tacgatgaag caacggaatt atttcagcat gttcgggtgt
    35221 tgaataactc ccctgatatt gcaagggcta ttcaggcaat tgatatcgag aaactctaaa
    35281 aaccatagtg atgtttttga caatgtgtta gctctttatc aataggccgc acggttgttt
    35341 ggtggcgtca aaatcgccga ggcgttccct gaaggccggg gcagcccaca tggatgtggg
    35401 ctgagggcgc gttttacagg gatgttacct cgcgcccgac ccggtagccg taagggataa
    35461 gccgagggca ccgcgcagcg gcgattttgt tcgccagagc ccgggggggc agggggcggc
    35521 ggcgattggt cgccccctgc gcgctccttg cgccagtgat tatatgaagc aatgcacatg
    35581 acaggagcgc aacaggatga gagactgaaa tattctcgtt attttcggat ctgatgacat
    35641 taattaattt taaaatatca tcttaaatac gccagtcacc accagtagcc caatcaggaa
    35701 aataatcaat acagcccaga gtataatttt cattggcgtt cctttatttg tgagtgctga
    35761 ataagtatag gaaaaaacga agccctttgc cgttatccat aaacgattaa gataaatcgg
    35821 aagatatcat ttacttagtc tatatttttc tgggtatgtc gttttattgc tactgatgta
    35881 ttgccacgcg aaactaaaaa atatatcgca acacacggta atctccgcgc accgtttctt
    35941 acctgattat tcccccatga atcgtgattc cttttatcca gccatcgcct gttttccgct
    36001 gttactgatg ctggccgggt gtgcgcctat gcatgaaacc cgccaggcgt taagccagca
    36061 aacgcccgct gcacaagttg acaccgcatt acccacggcg ctgaaaaatg gttggccaga
    36121 cagccaatgg tggctggagt atcacgataa tcagctcact tcattaatta acaatgcact
    36181 acagaacgca ccggatatgc aggtggctga acagcgcatt tagctagctg aagcccaggc
    36241 gaaggcagtt gccactcagg atggtccgca gatcgacttt tcggcggata tggagcggca
    36301 aaaaatgtcg gcagaagggc taatggggcc gtttgctctg aacgatccgg ccgcaggtac
    36361 gaccggtccg tggtacacca acggaacttt tggcttaacg gcgggctggc atctcgatat
    36421 ctggggaaag aatcgggcgg aggttactgc ccgcctgggt acggttaaag cacgggcggc
    36481 ggaacgcgag caaacccgcc aattgctggc tggcagcgta gcccgcctgt actgggagtg
    36541 gcaaacccag gcggcgttaa acacggtctt gcagcaaata gaaaaagagc agaacaccat
    36601 tatcgcgacc gatcgccagc tatatcagaa cgggattact tcttcagttg acggtgtgga
    36661 aaccgatatt aatgccagca aaacccggca gcagctcaac gatgtcgcgg ggaaaatgaa
    36721 aattattgag gcacggttaa atgcactcac aaatcaccag actaagtcat tgaagcttaa
    36781 accggtcgcg ttgccgaaag tggcaagcca gcttcctgat gaactggggt actccttact
    36841 ggcccggcgg gcagatttgc aggcggcgca ctggtacgtt gagtcatcgt taagcactat
    36901 tgatgcggca aaagcggcat tttatcctga catcaacctg atggccttcc tgcaacagga
    36961 tgcgttgcac ttaagcgatc tgttccgtca ttccgcgcag caaatgggcg ttacggcagg
    37021 cctgacgcta cctattttcg atagtggtcg tcttaacgcc aatctcgata tcgcaaaagc
    37081 cgaaagcaac ttgtctatcg ccagctacaa caaagcggtg gttgaagcag tgaatgacgt
    37141 ggcgcgggca gccagtcagg ttcagacact ggcggagaaa aaccagcatc aggcgcaaat
    37201 tgagcgcgat gccttgcgtg tggtaggtct tgcgcaggcc cgctttaacg cgggcatcat
    37261 tgctggttcc cgcgtcagcg aagccagaat cccggcgctg cgtgagcggg ccaatggcct
    37321 gttattgcaa gggcagtggc tggatgcctc cattcgactc accggtgcgc tgggcggggg
    37381 ttacaaacgc tgatgatatg cgccttctat acttaacgtt tattcagcgt taagtggaga
    37441 actcgatggc ataggtcgcg ataattaccg cttccgattc ggggatcggc aaagagtgcg
    37501 cgttattact ggcgcagcag gggtttgata ttggcattac ctggcactca gatgaagaag
    37561 gggcaaaaga taccgcgcgt gaggtggtta cccacggcgt acgtgcggag atcgtgcaac
    37621 tggatctcgg caatctgcca gaaggtgcgc aggcgctgga gacactcatc cagcgatttg
    37681 ggcgtattga tgtgctggtg aataatgcgg gtgcaatgac caaagcgccg tttcttgata
    37741 tggcttttga tgagtggcgc aagattttta ccgttgatgt tgatggtgca tttttatgct
    37801 cgcaaattgc ggctcgtcag atggtgaaat aagggcaggg cggtcgcatc atcaacatta
    37861 cgtcggtaca tgaacatacg ccgctgccgg atgccagcgc ctacacagcc gctaaacatg
    37921 cgctcggcgg gttaaccaaa acgatggcgc tggagttggt caggcataag attttggtga
    37981 acgcagtcgc gcctggggcg atagccacgc caatgaacag catggatgat agcgacgtga
    38041 agcccgacgc ggagccttcg attcctctac ggcgttttgg cgcaacgcat gagattgcca
    38101 gcctggtggt gtggctttgt tcggagggcg caaattacac gacagggcag tcgttgatag
    38161 tggatggcgg ctttatgttg gcgaatccgc agttcaaccc agaatagcgc agtgaggtgc
    38221 gtggatattt caatgggtta ggccagataa gacaccagcg tcgcatcagg catggaacac
    38281 ccggtgccgg atgtggagtg aatgcttatc tgtggcaact gctttacgcc tgattatccg
    38341 gctttttctt cccgcgccgt ttcagccacc agcgcacgcc caccaccaga accacgacca
    38401 gaatcaacca gacccagtgc ttcaaatgct ggtcgagatt gtgcaaccac ggcgcaatca
    38461 cctgaccacc agcgtaacca atagtggtaa aaatcaacgc ccaggcaaat gcgccgagaa
    38521 tattcagcgg cagaaagatt ttcggcggca gctggctggc accaatcagc gtcgggccaa
    38581 tcacccgaaa gccatacata aagcgcgtac caatgacaaa cagatacgga tggcgttgga
    38641 taagtttctg tgcccgctca attttatcct gatgtttcga gaaacggcgt aacagcttgc
    38701 cgccaaaccg ccgcccgcac agatagagca cctggtcgcc aatcatgcca ccaagcgcca
    38761 cagaaagtac caccagtggg aactttaata gcccctgatg tgccgcaacg cctcccagca
    38821 aagtcacggt ttcaccttcc gccaggctac cgataaccag cgcggcataa ccatattgtg
    38881 agatgagtgt attgagatcc ataggccagt ttgtctccct ttggttgcct gtaagcatac
    38941 accctgtgaa tcattcacgc tgaaaaagga catacatcag acggtgcaaa ttacatggat
    39001 gaacaaaaaa taatcacaag ctggcttata cttgaactgt caggttcatg aactcacaac
    39061 aagggggcgg tatgagccat gtctggggac tgttttccca tcccgatcgt gaaatgcagg
    39121 tgattaatcg cgaaaacgaa acgatttctc accattacac ccaccacgtt ttgctgatgg
    39181 cggcgattcc ggtgatttgc gccttcattg gcactacaca gattggctgg aattttggcg
    39241 atggcactat cctgaagtta tcctggttta ccggactggc gctggctgtc ttgttttacg
    39301 gcgttatgct ggctggggtt gcggtgatgg ggcgggtcat ctggtggatg gcgcgcaatt
    39361 acccgcagcg tccgtctctt gcgcactgca tggtcttcgc gggctacgtt gcaactccgc
    39421 tgtttttaag cggtctggtg gcgctttatc cactggtctg gttgtgtgcg ctggtcggca
    39481 cggtggcgct gttttacacc ggctacctgc tttaccttgg tattccgagc ttcctgaata
    39541 tcaacaaaga ggaaggcctt agcttctcca gttcgacgct ggcgattggc gtgctggtac
    39601 tggaagtgct gctcacccta accgttatac tatggggcta cggttatcgt ttgttctaga
    39661 aagttctttg acgttgcatt gctggcgtaa atgtcagcaa tgcagcattt actcacgatt
    39721 ctcctttgac gatcgcactt tttgctcgtt atgatggcgt cgctagcctc agtaaatcct
    39781 tactccggcg gtgcgcaacc cgtgcgcgtg aaccactatc tgaatgctca tcatgccgaa
    39841 atttcgagtt tctttattta gcttggccct gatgctggct gtgccttttg caccgcaggc
    39901 cgttgcgaaa acggcagccg ctaccaccgc ttcacaaccg gaaattgcct ccggtagcgc
    39961 gatgattgtt gatctgaata ccaacaaagt gatctattcg aaccacccgg atctggtgcg
    40021 tccgattgcg tctatcagca aattaatgac cgcgatggtt gtgctggatg cacgactgcc
    40081 gctggatgaa aaactaaaag tggatatcag ccagacgccg gagatgaaag gggtctattc
    40141 gcgcgtacga ctgaatagcg aaatcagccg taaagatatg ctgttgctgg cgctgatgtc
    40201 ttcagaaaac cgcgcggcgg caagccttgc gcaccattat cccggtggtt acaaagcctt
    40261 tattaaggca atgaatgcga aagcgaaatc gctcggaatg aacaacacgc gctttgttga
    40321 acctaccgga ttgtcggtgc ataacgtttc aactgcccgt gacttaacca aactgctcat
    40381 tgccagcaaa caatatccgt tgatcgggca gttaagtact acccgggaag atatggcaac
    40441 cttctctaac ccgacgtata cgctgccgtt ccgcaatact aatcatctgg tgtatcgcga
    40501 taactggaat attcagttaa ccaaaaccgg ctttaccaat gcggcgggcc attgtctggt
    40561 gatgcgtacg gttatcaata ataaaccggt ggcgctggta gtgatggacg cgtttggcaa
    40621 atatacccat tttgccgatg ccagccgcct gcgtacctgg attgaaaccg gtaaagtgat
    40681 gcctgtgccg gcagcagcgt tgagctataa aaaacaaaaa gccgcccaaa tggcggcggc
    40741 ggggcagacg gcacagaacg attaagtgaa ttcggatggc gatactctgc catccgtaat
    40801 ttttactcca cttcctgcca gtttttccgt ttactggttt taccgatccc cggattcatg
    40861 ctattggtcg gatcattctc gcgatagaac ttctgcaacg tctccggtgc tttatacaaa
    40921 tgaccgacgt tatgctcggc agggtactgc gcgccgcgct gctgtagcag ttccagcatc
    40981 tgttctttta acgcatgcac atccacgcct tttttcacta tgtaatcctg atggaagaca
    41041 tagcacataa aatggccgta atagagcttg tgcaccagct ggctgtcgat ctccggcggt
    41101 aaatgctcat accactcggt gtcgttacgc cgcagagcga tatccagtgc cagaatgtct
    41161 tcgacttcat cggaatgcac cgcctgataa cgaattgctg cgcccgcagc ggcgaaacgg
    41221 tgtaaaaacg ctttgctacc ttcctccggc gtacagacaa agaaatcgcc ctcggcctgt
    41281 ttgaaataat ccaccagcca cgatttggct tcaccgacgc catcgcctgc catttttaac
    41341 agcagatgat gctcgtattt atcgcgccag tttttcatgc gcggcggtaa atggctgggg
    41401 aacaggtgac cgaatttttg cattgcacgg tcagtaaaat gcggacggaa gaatttcact
    41461 ttctccagca tcgcatcggt gcgtcccttg agattaaaga agaacggcat cttgtcggtg
    41521 cctaacttat caatcatcag gaaggtgtct ttgccgtatt tttccgcaat atcgtagata
    41581 tcccggtgca tatattcccc ggcaaccggc agattttcga agttagccag aatatgacgg
    41641 cggatttcgg tcagcacttc cggctggttg gtgccgatat aaaacacctg ctgatttttt
    41701 tccgcttcga aggtatcaag acgtaccgca aagacggcca gcttcccggc gcaaccagaa
    41761 gattcaaata accgatccgg atcggcgtta taacgtgcgg gcgtgtcggc ttcaatatca
    41821 cgaacgcggt ggacatagtc ataatcgtgg gcgtgacgac catcgtgacg cacatcgtca
    41881 tctttgatgc gatcgtcatc cagcttgcta aggatctgct ccggcgtttc gcccagatca
    41941 atccccagat ggttcaccag cgtcagtttg ccatcttcat ctatacgcgc gaataacgac
    42001 atttcggtat acgccgggcc acgttgcacc agcgagccgc cggagttgtt acaaataccg
    42061 ccgatgaccg atgcgcctat acacgatgat ccaatcaccg aatgcggttc gcgtcccagc
    42121 ggtttgaggg ctttttccag cgaatagagc gtggtgcccg gataggccag cacctgttcg
    42181 cccttgccga gaacgtgcag cttgtcgaga cgcagggtgc tgatgatcac gatatcgcga
    42241 tcatagtcgt taccgtttgg cgtcgatcct tcggtcaggc ctgtattggc agcctgcatc
    42301 agaataattt tgtcggcggt gacgcaggct ttcagcaccc gccacaattc cagtagtgag
    42361 ccagggaaaa cgacagccag cgcgtcgccc tgaccagaac ggaagccctt gcgatagcgg
    42421 gccgtttttg cgggatcggt gagcaggtgt gaatgaccga ccagacgggc aagttcattc
    42481 aaaaaggctt tattatcagt tgttgtcatg gaagacattt tccactcctt gtggtggcga
    42541 aaaaatatca caaactagaa tagcgccttg tatgacaatt cagcgagtga atattttgaa
    42601 aattcagaca ataaactgag cattcttttt ctctctccat catgcttatg gcaaactacg
    42661 gtttttgcac cattttcgct gccacgtcgg gcaacaaagg aagaaaaatc catatgaaat
    42721 ggctatgttc agtaggaatc gcggtgagtc tggtcctgca gccagcactg gcggatgatt
    42781 tattcggtaa ccatccatta acgcctgaag cgcgggatgc gttcgtcacc gatctgctca
    42841 agaaaatgac ggttgatgag aaaattggtc agctgcgctt aatcagcgta ggcccggata
    42901 atccgaaaga ggcgatccgc gagatgatca aagacggtca ggttggggcg attttcaaca
    42961 ccgtaacccg tcaggatatc cgcgccatgc aggatcaggt gatggaatta agccgcctga
    43021 aaattcctct tttctttgct tacgacgtgc tgcacggtca gcgcacggtg ttcccgatta
    43081 gcctcggtct ggcctcgtct tttaacctcg atgcggtgaa aacggtcggg cgtgtctctg
    43141 cttatgaagc ggcagatgat ggcctgaata tgacctgggc accgatggtc gatgtctcgc
    43201 gcgatccgcg ctggggacgt gcttccgaag gttttggcga agatacgtat ctcacctcaa
    43261 taatgggtaa aaccatggtg gaagcgatgc agggtaaaag cccggcagat cgttactcgg
    43321 tgatgaccag cgtcaaacac tttgccgcat acggcgcggt agaaggcggt aaagagtaca
    43381 acaccgtcga tatgagtccg cagcgcctgt ttaatgatta tatgccgccg tacaaagcgg
    43441 ggctggacgc aggcagcggc gcggtgatgg tggcgctgaa ctcgctgaac ggcacgccag
    43501 ccacctccga ttcctggctg ctgaaagatg ttctgcgcga ccagtggggc tttaaaggca
    43561 tcaccgtttc cgatcacggt gcaatcaaag agctgattaa acatggcacg gcggcagacc
    43621 cggaagatgc ggtgcgcgtg gcgctgaaat ccggaatcaa catgagtatg agcgacgagt
    43681 actactcgaa gtatctgcct gggttgatta aatccggcaa agtgaccatg gcagagctgg
    43741 acgatgctgc ccgccatgta ctgaacgtta aatatgatat ggggttgttt aacgacccat
    43801 acagccattt ggggccgaaa gagtctgacc cggtggatac caatgccgaa agccgcctgc
    43861 accgtaaaga agcgcgtgaa gtggcgcgcg aaagcctggt gttgctgaaa aaccgtctcg
    43921 aaacgttacc gctgaaaaaa tcggccacca ttgcggtggt tgggccactg gcggacagta
    43981 aacgtgacgt gatgggcagc tggtccgcag ccggtgttgc cgatcaatcc gtgaccgtac
    44041 tgaccgggat taaaaattcc gtcggtgaaa acggtaaagt gctgtatgcc aaaggggcga
    44101 acgttaccag tgacaaaggc attatcgatt tcctgaatca gtatgaagaa gcggtcaaag
    44161 tcgatccgcg ttcgccgcaa gagatgattg atgaagcggt gcagacggcg aaacaatctg
    44221 atgtggtggt ggctgtagtc ggtgaagcac aggggatggc gcacgaagcc tccagccgga
    44281 ccgatatcac tattccgcaa ggccaacgtg acttgattgc ggcgctgaaa gccaccggta
    44341 aaccgctggt gctggtgctg atgaacgggc gtccgctggc gctggtgaaa gaagatcagc
    44401 aggctgatgc gattctggaa acctggtttg cggggactga aggcggtaat gcaattgccg
    44461 atgtattgtt tggcgattac aacccgtccg gcaagctgcc aatttccttc ccgcgttctg
    44521 tcgggcagat cccggtgtac tacagccatc tgaacaccgg tcgtccgtat aatgccgaca
    44581 agccgaacaa atatacttcg cgttattttg atgaagctaa cggagcgctt tatccgttcg
    44641 gctatggtct gagctatacc actttcaccg tctctgatgt gaaactttct gcgccgacca
    44701 tgaagcgtga cggcaaagtg acggccagcg tgcaggtgac gaacaccggt aagcgcgagg
    44761 gggccacggt agtgcagatg tacttgcagg atgtgacggc ttccatgagt cgccctgtga
    44821 aacagctgaa aggctttgag aaaatcaccc tgaaaccggg cgaaactcag actgtcagct
    44881 tcccgatcga tattgaggcg ctgaagttct ggaatcaaca gatgaaatat gacgccgagc
    44941 ctggcaagtt caatgtattt atcggcactg attccgcacg cgttaagaaa ggcgagtttg
    45001 agttgctgta atgctttagt aagggcgcga cgtttgcgcc ctttgcctga tgttcatatc
    45061 ttaacttgct gaattttcgc cctttgtagg ccggataagg cgctcgcgcc gcatccggca
    45121 ggaaacgccg gaccgaatat gcctcttaag tatttatctg attctgcaac tgaatccttc
    45181 cgctcaggct aactctgtca ttatcaacta tgcttttctc ttaattcgct gaaaacaaca
    45241 gtaaaagaat gaggaaagca tcatgccact cttaaagctc tgggcaggtt cactggttat
    45301 gttggcagcc gtgagcctgc cgctacaggc ggcttccccc gttaaagtcg gttcaaaaat
    45361 cgataccgaa ggcgcgctgc tcggcaacat catcttgcag gtactcgaaa gccacggcgt
    45421 accgacggtg aataaagtgc aactcggaac gactcctgtg gggcgcgggg cgattacttc
    45481 cgatgaactg gatatctatc cggaatatac cggcaatggc gctttcttct ttaaagatga
    45541 aaacgatgca gcgtggaaaa atgccgggca gggctacgag aaagtcaaaa agctcgatgc
    45601 ggagcacaac aagttaatct ggctgacgcc cgcgcctgca aataacacct ggactatcgc
    45661 cgtgcgtcag gatgtggcag agaaaaacaa actcacttcg cttgccgacc tgagtcgtta
    45721 tctgcaagag ggcggcacct tcaaactggc agcctcggca gagtttatcg aacgcgccga
    45781 tgcgttaccc acgtttgaaa aagcctatgg ctttaagctc ggtcaggatc agttgctgtc
    45841 actggctggt ggcgacacgg cggtgacgat taaagccgct gcccagcaaa cctctggcgt
    45901 taatgccgca atggcttacg gcactgacgg tccggtcgcg gcgctggggc tgcaaacctt
    45961 aagcgatccg caaggcgtgc aacctatcta cgcgcctgca ccagtggtgc gtgaatcggt
    46021 gttgaaggag tatccgcaaa tggcacagtg gctacagcca gtcttcgcca gcctcgatgc
    46081 aaaaacattg cagcaactga atgccagcat tgcagtggaa ggactggatg ccaaaaaagt
    46141 ggctgccgac tacctgaaac aaaaagggtg ggcgaagtaa tttcccgtga cttatctacg
    46201 tattaatcct gttctggcgc tgctgttgtt gctgacggca atcgcagcgg cgctgccgtt
    46261 tatcagttac gcgcctaatc gtttagtctc gggtgaaggg cttcatctct ggcaactgtg
    46321 gccgcaaacg atctggatgc tggtgggcgt tggttgcgcc tggctgacag cctgttttgt
    46381 ccccgctaaa aaaggcagca tttttgcact cattctggcg caattcgtct tcgtattgtt
    46441 ggtgtgggga gctggaaagg cggcgaccca actggcgcaa aatggcagtg cgctggcgcg
    46501 taccagcctc ggcagaggtt tctggctggc tgcggcgctg gcattgctgg cctgtagcga
    46561 tgccatccgc cgaatctcca cgcatccgct gtggcgctgg ttgttgcata tgcagattgc
    46621 cattattccg ctgtggttgc tgtactccgg cacgcttaac gatctctcac taatgaaaga
    46681 atacgccaac cgtcaggatg tgtttgacga cgcgctggca caacatctga cgttgctgtt
    46741 tggtgcggtg ctgcctgcgt tagtgattgg tgtgccgttg ggcatctggt gctacttttc
    46801 cactgcgcgg cagggggcaa ttttttctct gctcaatgtc attcagaccg tgccttcggt
    46861 ggcgctcttt ggcctgttga ttgcgccgct tgccgcgctg gttacggcct ttccgtggct
    46921 ggggaagctc ggcatagcag gaaccggaat gacacccgca ctgattgcgc tggtgctcta
    46981 tgccttgctg ccgctggtgc gaggcgtggt agtcggtttg aaccagatcc cgcgcgatgt
    47041 gctggagagc gccagagcga tggggatgag cggggcgcag cgtttcctgc atgttcagtt
    47101 accgctggcg ttaccggtat ttctgcgcag cctgcgggtg gtgatggtgc aaactgtagg
    47161 tatggcggtg attgcggcgt taatcggcgc aggcggtttt ggtgcgctgg ttttccaggg
    47221 gctgctaagc agcgccattg atttagtgtt gctgggggtg atcccggtaa ttgttctggc
    47281 ggtgcttacc gacgcgctgt tcgatttgct tatcgcactg ctgaaggtga aacgtaatga
    47341 ttgaatttag ccatgtcagc aaactgttcg gcgcacaaaa agccgttaac gatctcaatc
    47401 tcaattttca ggaagggagt ttttcggtgc tgattggcac atctggctcc ggcaaatcca
    47461 ccaccctgaa aatgattaac cgcctggtgg agcatgacag cggagtgatc cgctttgccg
    47521 gagaagaaat tcgctcgctg ccagtactgg agttgcgccg ccggatgggc tatgccattc
    47581 aatctattgg cctgttcccc cactggagcg tggcgcaaaa catcgctacc gtgccgcaat
    47641 tacaaaaatg gtcgcgggcg cggattgacg atcgtatcga cgaattaatg gcgctactgg
    47701 ggctggagtc aaatttgcgt gagcgttatc cgcatcagct ttccggtggt cagcagcaac
    47761 gtgtgggagt ggcgcgtgca ctggctgccg atccgcaagt cttactgatg gatgaacctt
    47821 ttggcgcact ggacccggta acgcgcggcg cgttgcaaca agagatgacg cgcattcacc
    47881 gtttgctggg gcgcactatt gtgctggtca ctcatgatat tgatgaggcg ctacggctgg
    47941 cagaacatct ggtattgatg gatcacggtg aagtggtgca gcaggggaat ccgctgacga
    48001 tgctgactcg tccggcgaat gattttgtcc gccagttttt tggacgtagt gaactggggg
    48061 tgcacctgct ttcgttacgt agcgtggcgg attacgtgcg tcgcgaagaa cgggcagaag
    48121 gtgaggcact ggcagaagag atgacgctac gcgatgcgct ctccctgttt gtcgcgcggg
    48181 gatgcgaggt gctgccggtg gtgaacacgc agggccagcc ttgcggcacg ctgcattttc
    48241 aggatctgct ggtggaggcg taagcgtatg aagatgttgc gcgatccgct gttctggctc
    48301 attgctcttt ttgtggcgct gattttctgg ctgccttaca gccagccgct gtttgctgcc
    48361 ttgttcccac aactgccacg acccgtttat cagcaagaaa gttttgcagc tctggcactg
    48421 gctcatttct ggctggtggg aatttcgagt ttgtttgcgg tgatcattgg cacaggtgcc
    48481 ggaattgctg ttactcgccc gtggggcgcg gaatttcgcc cactggtgga aactattgcc
    48541 gccgttggac agacttttcc gcctgtcgca gtgctggcga ttgccgttcc ggtgatcggc
    48601 tttggtctgc aaccagcgat tatcgccttg atcctttacg gtgtgctgcc catcctgcag
    48661 gtgacacttg ccgggctggg agcgattgat cccagcgtga cagaagttgc gaaaggtatg
    48721 ggaatgagtc gaggtcagcg actgcgtaag gtcgagctac cgctggcggc tccggtgatt
    48781 ctggcgggcg tgcgaacttc ggtgattatc aacattggta cggcgacgat cgcctcaacg
    48841 gtaggggcca gtacgctggg tacgcccatc atcatcgggc ttagcggatt taataccgcg
    48901 tatgtgatcc agggggcgtt actggtggca ctggcggcga tcatcgcaga ccgcctgttt
    48961 gaaaggctgg tgcaggcgct tagccagcac gcaaaataaa ggtataacct gcgagcatga
    49021 cgccaccaat tccgcctaac gccataaaca ggaacagggc gatgacccca attttagcta
    49081 tgcgcataat gcactcctta tgttaacgaa aggattgtac agtaaagcgc atttgttaac
    49141 gaatcattaa ataccgagtg ggaaaatatc atggccttgt tcttgccaac tggtgagttg
    49201 ctgctgttgg gcggaggttc gattttcacc gcaccacacc agcaatgtac ggccttcgaa
    49261 tagttcaggg cgtagttgat tgagcgagtg ggcgagcgag gacatcaatg cgccatcctt
    49321 gttgactggc aatccagccc tccagccaca gacgggtggt atcctgaata ttccagccaa
    49381 ccaccagcgc atctttaccc tgttttttac gtgccgaagc cagacaaatg gcgatgtagt
    49441 tgatcagtac gccgtcgagg atcgccagca gcgcctggag agtcggttgt tg
//
LOCUS       DAJTRN010000019        48321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRN010000019 DAJTRN010000000
VERSION     DAJTRN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            187..480
                     /locus_tag="OO328_001134"
     CDS             187..480
                     /locus_tag="OO328_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5929773.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO328_001135"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO328_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5929774.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO328_001136"
     CDS             complement(1922..2374)
                     /locus_tag="OO328_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5929775.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO328_001137"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO328_001137"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5929776.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO328_001138"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO328_001138"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5929777.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO328_001139"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO328_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5929778.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO328_001140"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO328_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5929779.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO328_001141"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO328_001141"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5929780.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO328_001142"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO328_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5929781.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO328_001143"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO328_001143"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5929782.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO328_001144"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO328_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5929783.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO328_001145"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO328_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5929784.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO328_001146"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO328_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5929785.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO328_001147"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO328_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5929786.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO328_001148"
     CDS             complement(15085..15582)
                     /locus_tag="OO328_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5929787.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO328_001149"
     CDS             complement(15579..15941)
                     /locus_tag="OO328_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5929788.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO328_001150"
     CDS             complement(15931..16278)
                     /locus_tag="OO328_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5929789.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO328_001151"
     CDS             16387..16800
                     /locus_tag="OO328_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929790.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO328_001152"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO328_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO328_001153"
     CDS             complement(18324..20039)
                     /locus_tag="OO328_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5929791.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO328_001154"
     CDS             20206..21099
                     /locus_tag="OO328_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5929792.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO328_001155"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO328_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OO328_001156"
     CDS             complement(21910..23526)
                     /locus_tag="OO328_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5929793.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="OO328_001157"
     CDS             23821..24495
                     /locus_tag="OO328_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5929794.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="OO328_001158"
     CDS             complement(24534..26195)
                     /locus_tag="OO328_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5929795.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO328_001159"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO328_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5929796.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO328_001160"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO328_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5929797.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="OO328_001161"
     CDS             27649..27981
                     /locus_tag="OO328_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5929798.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="OO328_001162"
     CDS             complement(27983..29215)
                     /locus_tag="OO328_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5929799.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="OO328_001163"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="OO328_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5929800.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="OO328_001164"
     CDS             complement(30377..31714)
                     /locus_tag="OO328_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5929801.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO328_001165"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO328_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO328_001166"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO328_001166"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5929802.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO328_001167"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO328_001167"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5929803.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO328_001168"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO328_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5929804.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /locus_tag="OO328_001169"
     CDS             35377..36033
                     /locus_tag="OO328_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929805.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO328_001170"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO328_001170"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5929806.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="OO328_001171"
     CDS             complement(37005..37403)
                     /locus_tag="OO328_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5929807.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO328_001172"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO328_001172"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5929808.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO328_001173"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO328_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5929809.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO328_001174"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO328_001174"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5929810.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO328_001175"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO328_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5929811.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="OO328_001176"
     CDS             43964..44044
                     /locus_tag="OO328_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929812.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="OO328_001177"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="OO328_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5929813.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="OO328_001178"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="OO328_001178"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5929814.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="OO328_001179"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="OO328_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5929815.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="OO328_001180"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="OO328_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5929816.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="OO328_001181"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="OO328_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5929817.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13065 c        12219 g        11057 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DAJTRN010000020        47646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRN010000020 DAJTRN010000000
VERSION     DAJTRN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(56..673)
                     /gene="tdk"
                     /locus_tag="OO328_001182"
     CDS             complement(56..673)
                     /gene="tdk"
                     /locus_tag="OO328_001182"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5929818.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1278..1691
                     /gene="hns"
                     /locus_tag="OO328_001183"
     CDS             1278..1691
                     /gene="hns"
                     /locus_tag="OO328_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5929819.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1835..2743)
                     /gene="galU"
                     /locus_tag="OO328_001184"
     CDS             complement(1835..2743)
                     /gene="galU"
                     /locus_tag="OO328_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5929820.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2945..3958)
                     /gene="rssB"
                     /locus_tag="OO328_001185"
     CDS             complement(2945..3958)
                     /gene="rssB"
                     /locus_tag="OO328_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5929821.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4050..4955)
                     /gene="rssA"
                     /locus_tag="OO328_001186"
     CDS             complement(4050..4955)
                     /gene="rssA"
                     /locus_tag="OO328_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5929822.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5068..5526
                     /locus_tag="OO328_001187"
     CDS             5068..5526
                     /locus_tag="OO328_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5929823.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5576..6418
                     /gene="purU"
                     /locus_tag="OO328_001188"
     CDS             5576..6418
                     /gene="purU"
                     /locus_tag="OO328_001188"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5929824.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6578..6662
                     /locus_tag="OO328_001189"
     tRNA            6578..6662
                     /locus_tag="OO328_001189"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6612..6614,aa:Tyr,seq:gta)
     gene            6709..6840
                     /locus_tag="OO328_001190"
     ncRNA           6709..6840
                     /ncRNA_class="other"
                     /locus_tag="OO328_001190"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7024..7701)
                     /gene="narI"
                     /locus_tag="OO328_001191"
     CDS             complement(7024..7701)
                     /gene="narI"
                     /locus_tag="OO328_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5929825.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7701..8411)
                     /gene="narJ"
                     /locus_tag="OO328_001192"
     CDS             complement(7701..8411)
                     /gene="narJ"
                     /locus_tag="OO328_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5929826.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8408..9946)
                     /gene="narH"
                     /locus_tag="OO328_001193"
     CDS             complement(8408..9946)
                     /gene="narH"
                     /locus_tag="OO328_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5929827.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9943..13686)
                     /locus_tag="OO328_001194"
     CDS             complement(9943..13686)
                     /locus_tag="OO328_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5929828.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14079..15470)
                     /gene="narK"
                     /locus_tag="OO328_001195"
     CDS             complement(14079..15470)
                     /gene="narK"
                     /locus_tag="OO328_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5929829.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15809..17605
                     /gene="narX"
                     /locus_tag="OO328_001196"
     CDS             15809..17605
                     /gene="narX"
                     /locus_tag="OO328_001196"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5929830.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17598..18248
                     /gene="narL"
                     /locus_tag="OO328_001197"
     CDS             17598..18248
                     /gene="narL"
                     /locus_tag="OO328_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5929831.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18249..19643)
                     /gene="ychO"
                     /locus_tag="OO328_001198"
     CDS             complement(18249..19643)
                     /gene="ychO"
                     /locus_tag="OO328_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5929832.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19828..20181
                     /gene="ychN"
                     /locus_tag="OO328_001199"
     CDS             19828..20181
                     /gene="ychN"
                     /locus_tag="OO328_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5929833.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20225..20920)
                     /gene="chaC"
                     /locus_tag="OO328_001200"
     CDS             complement(20225..20920)
                     /gene="chaC"
                     /locus_tag="OO328_001200"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5929834.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21078..21308)
                     /gene="chaB"
                     /locus_tag="OO328_001201"
     CDS             complement(21078..21308)
                     /gene="chaB"
                     /locus_tag="OO328_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5929835.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21578..22678
                     /gene="chaA"
                     /locus_tag="OO328_001202"
     CDS             21578..22678
                     /gene="chaA"
                     /locus_tag="OO328_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5929836.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23083..23190
                     /locus_tag="OO328_001203"
     CDS             23083..23190
                     /locus_tag="OO328_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5929837.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23339..24193)
                     /gene="kdsA"
                     /locus_tag="OO328_001204"
     CDS             complement(23339..24193)
                     /gene="kdsA"
                     /locus_tag="OO328_001204"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5929838.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24229..25038)
                     /locus_tag="OO328_001205"
     CDS             complement(24229..25038)
                     /locus_tag="OO328_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5929839.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25042..25434)
                     /locus_tag="OO328_001206"
     CDS             complement(25042..25434)
                     /locus_tag="OO328_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5929840.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25431..26264)
                     /gene="prmC"
                     /locus_tag="OO328_001207"
     CDS             complement(25431..26264)
                     /gene="prmC"
                     /locus_tag="OO328_001207"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5929841.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26264..27346)
                     /gene="prfA"
                     /locus_tag="OO328_001208"
     CDS             complement(26264..27346)
                     /gene="prfA"
                     /locus_tag="OO328_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5929842.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27388..28644)
                     /gene="hemA"
                     /locus_tag="OO328_001209"
     CDS             complement(27388..28644)
                     /gene="hemA"
                     /locus_tag="OO328_001209"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5929843.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            28858..29481
                     /gene="lolB"
                     /locus_tag="OO328_001210"
     CDS             28858..29481
                     /gene="lolB"
                     /locus_tag="OO328_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5929844.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29481..30332
                     /gene="ispE"
                     /locus_tag="OO328_001211"
     CDS             29481..30332
                     /gene="ispE"
                     /locus_tag="OO328_001211"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5929845.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30483..31430
                     /gene="prs"
                     /locus_tag="OO328_001212"
     CDS             30483..31430
                     /gene="prs"
                     /locus_tag="OO328_001212"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5929846.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31555..33234
                     /gene="dauA"
                     /locus_tag="OO328_001213"
     CDS             31555..33234
                     /gene="dauA"
                     /locus_tag="OO328_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5929847.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33289..33567)
                     /gene="ychH"
                     /locus_tag="OO328_001214"
     CDS             complement(33289..33567)
                     /gene="ychH"
                     /locus_tag="OO328_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5929848.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            33845..34429
                     /gene="pth"
                     /locus_tag="OO328_001215"
     CDS             33845..34429
                     /gene="pth"
                     /locus_tag="OO328_001215"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5929849.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34546..35637
                     /gene="ychF"
                     /locus_tag="OO328_001216"
     CDS             34546..35637
                     /gene="ychF"
                     /locus_tag="OO328_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5929850.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36481..39348
                     /locus_tag="OO328_001217"
     CDS             36481..39348
                     /locus_tag="OO328_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5929851.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39448..41367)
                     /gene="dhaR"
                     /locus_tag="OO328_001218"
     CDS             complement(39448..41367)
                     /gene="dhaR"
                     /locus_tag="OO328_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5929852.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41595..42665
                     /gene="dhaK"
                     /locus_tag="OO328_001219"
     CDS             41595..42665
                     /gene="dhaK"
                     /locus_tag="OO328_001219"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5929853.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42676..43308
                     /gene="dhaL"
                     /locus_tag="OO328_001220"
     CDS             42676..43308
                     /gene="dhaL"
                     /locus_tag="OO328_001220"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5929854.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43319..44737
                     /gene="dhaM"
                     /locus_tag="OO328_001221"
     CDS             43319..44737
                     /gene="dhaM"
                     /locus_tag="OO328_001221"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5929855.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45057..46754
                     /gene="treA"
                     /locus_tag="OO328_001222"
     CDS             45057..46754
                     /gene="treA"
                     /locus_tag="OO328_001222"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5929856.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(46833..47272)
                     /locus_tag="OO328_001223"
                     /pseudo
     CDS             complement(46833..47272)
                     /locus_tag="OO328_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11952 a        12271 c        11667 g        11756 t
ORIGIN      
        1 aacgaactga caggataaaa gtgccctgct caccctgtca gtaaagaaat tcttattaat
       61 cgtggcgatg cctttcctga atagccgtta atgagccgac ttgtaacgcc tctttatagt
      121 gtttacggca tacagaaacg tatcgttcat taccaccaat taccacctgc tcaccttcgt
      181 tataaggtct gcctgcttga tcaagacgca gcaccatgct tgctttacgg ccacaaaaac
      241 agatggtttt taattcaacc agtttgtcgg accatgccag taagtattgg ctgccaataa
      301 ataattcacc tcgaaaatcg gtacgtaaac cataacaaag tacgggtata tcgagttgat
      361 cgacaacctc cgataattca tatacttgtt gtctggttaa aaactggcat tcatcaacca
      421 gtacgcaatg aattgcctgc tgttcatgtt ccgcacgaat ctcatcaaat aatgatgaat
      481 tttggttaaa taattttgca ggcgatgaca aacctatacg cgaactgact ttcccggcac
      541 caaagcgatc atcaatttct gccgtatata cgacagtgcg catgccgcgt tcctggtaat
      601 tgtatgaaga ttgcaacaat gctgtagact tacccgcatt cattgcggaa tagtagaaat
      661 atagctgtgc catcagccac aggccctcaa tgatatgaaa taaagttggc tggagtttat
      721 cataattcgt cagtttttca gctcaacagt atgcacagaa gtgggtatat gcgttctccc
      781 ttacgaagcc ttgcataatc cttctgagct atcattacaa ctgcctcgct tgttataagc
      841 gggtaaatga ctgctggtaa ctattcacaa tctttaacct gttgcgcaag taatagccct
      901 ctgttgacct ccaggagata gtgcaatact aagtccatgc tcttattgcg actgttctac
      961 ttttcatcat tcgcttaata gggaattctc gtaaacacaa ctaatacaga agactgaaag
     1021 gtcgtcagcc tacgataatc tccccataaa atgtgacatg aatcaggaag ttttaacctc
     1081 acgtgctgcg aaatcatcgg tgtaaatagg gctatatgcc gcgtcttttc tggctaattt
     1141 tatgaaaaga tatttattgg cggcacaaaa taaagaacaa ttttgaattc cttacattcc
     1201 tggctattgc acaactgaat ttaaggctct attattacct caacaaacca ccccaatata
     1261 agtttgagat tactacaatg agcgaagcac ttaaaattct gaacaacatc cgtactcttc
     1321 gtgcgcaggc aagagaatgt acacttgaaa cgctggaaga aatgctggaa aaattagaag
     1381 ttgttgttaa cgaacgtcgc gaagaagaaa gcgcggctgc tgctgaagtt gaagagcgca
     1441 ctcgtaagct gcagcaatat cgcgaaatgc tgatcgctga cggtattgac ccgaacgaac
     1501 tgctgaatag ccttgctgcc gttaaatctg gcaccaaagc taaacgtgct cagcgtccgg
     1561 caaaatatag ctacgttgac gaaaacggcg aaactaaaac ctggactggc caaggccgta
     1621 ctccagctgt aatcaaaaaa gcaatggatg agcaaggtaa atccctcgac gatttcctga
     1681 tcaagcaata atcttttgta gattgcactt gcttaaaatc ccgccagcgg cgggattttt
     1741 tattgtccgg tttaagacaa tttaataaga ataagctata aaaaaacggc gtcgattgct
     1801 caacgccgtt tcgtggataa caccgatacg gatgttactt cttaatgccc atctcttctt
     1861 caagccaggc tttaaattcc gtgccaaggg tgttatgacg aataccgtat tcaacgaagg
     1921 cctgcatgta acctaattta ttaccgcagt catggctctt ccctttcata tgataggctt
     1981 ccaccgtttc tttttcgatc agcatatcaa ttgcgtcggt gagctgaatt tcatcaccag
     2041 ctcccggagg ggtttttgcc agcaacggcc aaatatccgc gctaagtacg taacgaccca
     2101 caatagcgag attagacggc gcaacatccg ctttcggttt ttctaccaca ccaaccatcg
     2161 gtacgctttc acccggcgct aattcaacgc ctttgcaatc cacaacgcca tatgcggtca
     2221 catcagcaac cggttcaacc atgatctggc tatgacccgt ttcatcaaag cggcggatca
     2281 tctctgccag gttatcctgt gacaaatcgg attcatattc atccagaata acatcaggca
     2341 aaataacagc taccggttca tcacccacta ccgggtgagc acacaatacc gcgtgtccca
     2401 ggcctttcgc cagaccctga cgaacttgca taatagtcac gtgcggtgga caaatagact
     2461 gcacttcatc aagcagttga cgttttacac gtttttccag cattgcttcc agttcaaaac
     2521 tggtatcaaa gtggttttca atagagtttt tagatgagtg tgtaaccagc acaatttcag
     2581 taatgccagc cgcaatacat tcattcacga cgtattgaat taatggctta tcgacaagtg
     2641 gcagcatctc tttcgggatg gctttcgtcg ccggcaacat cctggttcct aatcccgcaa
     2701 cggggataac ggcttttttg actttcgtat taatggcagc catttaaatt ctcctggact
     2761 gttcgtgttt tgaacgtgtt catatttctg tatcgcatcc cagtatatca gcaccccgaa
     2821 ggggctaagt tctgaaaagg aggcgagata gcataattaa gacaattact tatacgaatg
     2881 gcgacgatag taccaccagc cgacattagc aggtaatgca aatttagccc gcgttatcgt
     2941 ttgctcattc tgcagacaac atcaagcgca gtcgaccacc ggttccccat atttggcatt
     3001 gccaggcatc gcatcgctgg ctcaattgat tcaaataagc gttacctaaa gtgcctaacg
     3061 gaacaccatt actgatttgc acctggtgtt cgccggtatt taacgtcgca ttcagacccg
     3121 cagaaaccag aatgagattt ttcagttcgc gatgataata gccaactaat agcggaaact
     3181 gccccggcag attggcctga cgaagtaaat ggtttacctg cttcaataac gcgcccaact
     3241 ctggcaaccg ttgattttgg tgtgcaagct gttcctgtaa taatccgtta aacaatgcgc
     3301 gtaataacaa ggcagcaagt acgccattat gtccagctcg ggtgacatca aggcaataaa
     3361 atgccagatc gttttccgaa agtgcggcaa tatcaagcac caggccgggt ttgtccgcgg
     3421 caaccaattg acggtaatta acccggcaat gggaaatcac ctgctgaacc ggcggttgta
     3481 gttcctgtaa taatttcgcc gctgcggcag ggttatcaac cattgcatcc cagtcgcgaa
     3541 aaagcctttc ctcttcctca acgcgcgaat taaacatgct gggatagaga caggcaaaaa
     3601 ccatctcgcg caagcgattc agatctttaa ctggtttcag caaaacatct tcaacgccca
     3661 gacgtaacgc tttggcaata tctgccatat tttcagtggc agatatcacc agaactgggg
     3721 tctggtcgcc tctgttacgt atatgctcca gcagtttaag cccgttcatt cgtggcatcg
     3781 cgatatcaca tatcatcagg tctggagtga aacctcccag caactcaagg gcatccaccc
     3841 catcagccgc cagtaccgtt gtcgctccca atgaggaaaa ccatgaatcc agaagcgagc
     3901 gaaatacctg ctcatcttca acaatgagaa tctgttttcc gaccaatggc tgcgtcatgt
     3961 tctctcccct gactggcttt actcaatagt ggcatgctat tgccacctgc gcctgtcaga
     4021 attaacttaa gtgtagataa aaattctggt caaatgttgg tgcgtaccaa cggcaaaagt
     4081 tcgtccattt tcctttccac tgccagctgt ccggccgcaa tggcagcgtg cgcgcgatgg
     4141 aaatcaagcg tagatatttg cgggcaaaca ggttgaatca gaatatcggg cggatcacct
     4201 gccatgcggt tccttttaag gcggttctcc aacacctgga tagacgtggt cataatctct
     4261 gttgccgttg gcgctgtcac agcccgacgt gtcgttatgc tgcctaacct ttctttcaga
     4321 cgcgcatgcc acggtagaga atcaccattc tcgctatttt cttcactgac attaaaggag
     4381 agcagatctt gttgcatcaa atgagcatcg tgctgcaggt cgaccgctat cacaatatca
     4441 gcccccaatg cacgcgtaag agaaatagga attgggttaa ctactgcccc atcaaccagc
     4501 cagtagccgt tatgtgcaac gggtgccatg agtcctggaa tactgcatga tgcgcgaata
     4561 gcaagatgga gatcgccttc agtaaaccat aattcacgcc ccgtacttaa attggtggca
     4621 acagccgcaa agcgacggga acaattttcg atctctgttt ccggcattat ttcgcgatat
     4681 tgattgaaga cacgctcgcc gcgcagtaac ccaccgcgct gccaggagag gtccatcagg
     4741 cgtaaaacgt cccaataact gaaagaggtt acccaatctt ccagcgcaga taatcgatcg
     4801 catgcatagg ccgcgcccac cagcgaacca attgaacatc ctgcaacgat atcaatttca
     4861 atacccactt tttttagcgc attaataacg ccaatatgcg accaacctct cgccgcgcca
     4921 gatcccagcg ccagccctat ttttatcttt ctcattattc ccgcaagatt cccctggaat
     4981 gcaatcgtag ccacatcgct actgctcggt taacatagtg ctactcaggc ggttaatacg
     5041 ccgttattgt ttccagggag attatttgtg tctcagcttt gtccctgtgg tagtgctgtc
     5101 gagtatagcc tatgttgcca cccttatgtg tctggtgaaa aggttgcacc tgatccagaa
     5161 catctcatgc gttcgcgtta ctgcgctttt gtgatgcaag acgctgatta tttaataaag
     5221 acctggcatc cctcttgtgg agcagcggcg ttacgtgccg aattgatgac cggatttgcg
     5281 catactgaat ggttgggatt gaccgtattt gaacattgtt ggcaggacgc cgacaatatc
     5341 ggatttgtca gttttgttgc tcgatttacc gaaggtggca aaacaggtgc aataattgaa
     5401 cgttcgcgat ttttgaaaga aaacggtcaa tggtactata ttgacggtac acgtccgcag
     5461 tttggtcgca acgatccctg cccttgtggt tcaggtaaaa aatttaaaaa gtgctgcggc
     5521 caataatggt tgacggtacg gtttagcaaa cactctcaac aaggtttttc cagcaatgca
     5581 ttcactccaa cgtaaagttc tgcgtactat ttgtccggac caaaaaggtc tgatcgcacg
     5641 tattaccaat atttgctaca agcacgagtt aaatatcgta cagaacaatg aatttgttga
     5701 tcaccgtacc gggcgctttt ttatgcgcac ggaactggaa gggattttta atgattccac
     5761 cctgctggcg gatctcgata gcgcattgcc agaaggctcc gtgcgtgagc tgaatcctgc
     5821 cggtcgtcgc cggatagtga ttctggtcac taaagaagcg cattgccttg gcgatttgtt
     5881 gatgaaagcc aattacggcg gtctggatgt cgaaatcgcg gcagttattg gtaaccacga
     5941 tactttacgt tctctggttg agcgttttga tattccgttt gagctggtaa gccatgaagg
     6001 gttaagccgc aacgagcacg atcaaaagat ggcggacgcc attgatgctt atcaacctga
     6061 ctatgtggtg ctggcgaagt atatgcgggt attaacgccg gaatttgtgg cacgcttccc
     6121 gaataaaatc atcaatattc accattcatt cctgcctgcg tttatcggcg cacgtcctta
     6181 tcaccaggcc tatgaacgtg gtgtgaagat tattggcgca accgctcact atgtgaatga
     6241 caatctggac gaaggcccaa tcatcatgca ggacgttatt catgtcgatc atacctacac
     6301 agctgaagat atgatgcgcg caggtcgtga cgtcgagaaa aacgtcttaa gtcgtgcgct
     6361 atacaaagta ctggcgcaac gcgtctttgt ttacggtaat cggacgatta ttctttaatc
     6421 gccagcaaaa ataactggtt acctttaatc cgttacggat gaaaattacg caaccagttc
     6481 atttttctca acgtaacact ttacagcggc gcgtcatttg atatgatgcg ccccgcttcc
     6541 cgataaggga gcaggctagt aaaaagcatt accccgtggt ggggttcccg agcggccaaa
     6601 gggagcagac tgtaaatctg ccgtcatcga cttcgaaggt tcgaatcctt cccccaccac
     6661 catcactttc aaaagccccg gaattctcaa acgaatctgc aatcaaatat tctgcccaag
     6721 cggggaagga tgagaagctt cgaccaaggt tcgacttgag cgccagcgag agagcgttgc
     6781 cgcaggcaac gacccgaagg gcgaagcgcg cagcgctgag taatccttcc cccaccacca
     6841 tcgcttcctt gaataatcaa aacctgttac tccgagctaa ttttgcccga tggcaccacg
     6901 tttaccgggc ctacaatatc ctcccctgta ggtcggataa gcgaagcgca tccgacaact
     6961 caccccgcac cgtgcccata aaaaaacccc gccgaagcag ggtctatgtg aactaaaatt
     7021 cgtttagtga cgagcgcgca ccagctggta cttacgtgtc agatactcca ccggtacgct
     7081 ccagatgtgt accagacgcg agaacgggaa cagcaggaat aacgtcatcc ccagcaccag
     7141 gtgcagacgg aagataaacg ccacaccatc gaggtgttga gaagcgccac cgtggaaggt
     7201 caccaccgac tgcgcccagc caaccagttt catcatctcg ctaccgtcca tatgctgagc
     7261 ggagaacgga atggtcagca ggcccagcgc gcactggata acgagcagcg acaggatcag
     7321 gatatccgct ccggtagtgg ttgcacgcac gcgtgggctg aacatacgac gtttcagcaa
     7381 aagcacgccg ccaatcagac acagcacgcc gctggcacca ccagcaaaca tcgccatttt
     7441 ctgtttcact tcaatcggca gccaggcttc atacatccag tgcggcgtca acataccgaa
     7501 gaagtgaccg acaaaaatcc ccagaatacc gatatggaac aggttcgacg ccaggttcat
     7561 ccctttgcga tccagcatct ggctggacgc cgcgcgccag gtgtactgcc cgtagtcata
     7621 acgcagccag ctaccaatca ggaagaccgc cccggctatg tacgggtaga tatcaaagaa
     7681 gaacatattc aggaattgca ttagtgctgt cctccggtgg tgatattcag atattgcggc
     7741 gcgacggcac cggcaaagcg acgctgatga gcagtgattg ctgaatcacc gcagcctttg
     7801 tcagcaaaga atttaacctg ctcttcttcc cagacagcat ccagcgcctg cggcgtatca
     7861 tcgcgcgctt cgtcggcaat tttttccgcc actttgtcgc tgtcgatagc ggtattggcc
     7921 aatttcagca gcagatcaaa catcacggca taacggcttt cacgctgttg cagacgcgcg
     7981 ctcagcaatg ccagaatcgg cgcgatatct ttcaaacctt ccacggcttc gctttgcggc
     8041 agctgcgaca ggtactccag atacagcggc agatggtccg gtaattcgcg gctgtttaac
     8101 tgcaagccgt gctgctcgta ctgcgccagc aggtccacca tcgcctggcc gcggtcacgg
     8161 gattcaccgt gaacatgttc aaatagcaac agtgacgtgg cacggccacg gtcgaacagt
     8221 tcgctgtact ggacctgggc atcgagcgga tccatcgccg ttaaatcgcg caggaaaatg
     8281 cccagcgcat gggcatcttc tttcgacaga tttttcgacg cagcaatcgc ctcaaacatc
     8341 tcttgttgat gctgccataa ggcagcatcc ggatattcaa ggagacgcga tacaatcacg
     8401 agttcgatca tggatgcggc tccgttttgc tggtcacatc gatggcatcg atacgacggc
     8461 tgttgaacag attgaatttg gtatctgagc cgtggcagcc atcaccaaag gtaaagccgc
     8521 agccattttt ctccgggaag gcttcccgtg ccagttcacg atgactactc ggcaccacaa
     8581 agcgatcttc gtagttagca atcgccagat aacggtacat ctcctgtgcc tgggcttcgg
     8641 tcagaccgac ctcttccagc gcacgggtat caactttacc gtcaacagtt tcagcacgtt
     8701 tgtaatgacg catcgccagc atacgtttca gcgcacgcag taccggtttg gtatcaccgg
     8761 cggtcagcag attcgccaga tactgtaccg gaatacgcaa gctttcgacg tctggcagaa
     8821 ttccgttgct gcccagctcg cccgcgtctg ctgcagactg aatcggagac agaggcggca
     8881 cgtaccagac catcggcagt gtgcgatatt ctggatgcag cggcagcgcc agtttccatt
     8941 ccatcgccat tttataaacc ggcgactgct gtgcagcttc aataacgctc agcggaatac
     9001 cgtctttaat cgcctgctcg atgactttcg gatcgttcgg atcgaggaac acgtccagct
     9061 gacgctggta gagatctttc tcgttctcgg tgctggctgc acgttcaatc gcgtcggcat
     9121 cgtacagcag cacgccaaga taacggatac gaccgacaca ggtttctgag cacacggtcg
     9181 gctgacccgc ttcaatacgc ggatagcaga agatgcactt ctcagactta ccgctcttcc
     9241 agttgaagta gatttttttg tacgggcatc cggtgatgca catacgccag ccacggcatt
     9301 tatcctggtc gatcaggacg atgccatctt cttcacgctt gtaaatcgca ccgctcgggc
     9361 aggtcgccac acatgccggg ttcaggcagt gttcgcacag gcgcggcaaa tacatcataa
     9421 aggtgttttc gaactggcta tacatcgcct tctggatgtt gtcgaagttc ttgtctttcg
     9481 ccagtttatc aaactcaccg cccaggtcat cttcccagtt cggccctttt tcgattttcg
     9541 ccatccgttc cccggtaatc agcgaacgcg gacgggcaat cggctgcgat ttgctgcctt
     9601 ccggcgcggt atgcaggttc tgataatcaa aatcgaacgg ctcgtaataa tcgtcgatcc
     9661 ccggcagatg cgggttagca aagattttac ccagcagcat ggctcggtta cccatgcgcg
     9721 gttgcagttt gccattgatt ttacggatcc agccgccttt gtatttttcc tggttttccc
     9781 agtcagtcgg gaagccctgg cccggcttgg tttccacgtt gttgaaccac gcgtattcca
     9841 cgccttcacg gctggtccag acgtttttac aggtaactga acaggtgtgg cagccgatgc
     9901 acttatcgag attcagcacc atgccgactt gtgaacgaat tttcatttta cgctctcctg
     9961 tacctggtca ttgccttcgc catctaacca gtcaatgttc ttcatcttac gcactacaac
    10021 aaactcatcg cggttagaac ctacggtgcc gtagtagtta aagccgtatg ccagatgggc
    10081 atagccgccg atcatatgcg tcggtttcgg cgtgatacgg gtgaccgagt tatggatacc
    10141 accacgctgt tgggtaattt ccgaaccagg caggttaacg atacgttcct gcgcgtggta
    10201 catcatggtc atccctgccg gaacacgctg gctgacaacc gcacgggcag tcagtgcacc
    10261 gttgctgttg aagacttcaa tccagtcgtt atcggcgata cccagatctt tggcatcggc
    10321 ttcactcaac cagaccaccg gaccaccgcg acctaaagtc agcatcagca ggttgtcgct
    10381 gtaggtggag tggatacccc acttctggtg cggcgtcagg aagttgagcg ctttttccgg
    10441 gttgccgttg gatttctggc ctatcacttc tttcaccgaa cgggtgtcga tcggcggacg
    10501 ataaaccagc aggctttcac cgaaatcacg catccactgg tgatcctgat acagttgctg
    10561 acgaccagag agcgtacgcc atgggatcag ctcgtgaacg ttggtgtaac cggcgttgta
    10621 agaaacgtgt tcatcttcca gaccagacca ggtcgggctg gagataattt tgcgcggctg
    10681 tgcctgaata tcgcggaagc ggatcttctc gtcttcttta ttcagcgcca gatgcgtatg
    10741 gtcacgaccg gtaaattcgc tcagggcagc ccaggctttc acggctacct gaccgttggt
    10801 ttccggtgcc agcgtcagga tcatctctgc cgcatcaatt gcggtgttca gcatcggctg
    10861 gccttttgcc ggaccttccg ctttggtgta gttgagctta cgcagcagat ccatctcgct
    10921 ctgggtgttc caggcaatcc ctttaccgcc gttaccgatt ttctccatca gcgggccgat
    10981 agaggtaaag cgttcgtaag ttgccggata atcgcgctct acgaccataa tgtgcggtgc
    11041 agttttgcct gggatcaggt cgcattcgcc ttttttccag tctttcacat ccagcggctg
    11101 cgccagttca gcggcagagt cgtgctggat aggcagcgtg acgatgtcgg tttctttacc
    11161 cagatagccg acgcacactt cggagaattt cttcgcgatg gctttgtaga tttcccagtc
    11221 gcttttcgct tcccaggccg gatcgaccgc cgcagacagc gggtgaataa acggatgcat
    11281 atccgaagta ttcatgtcgt ctttttcgta ccaggtcgcc gtcggcaaaa tgatgtcgga
    11341 atagagacag gtgctcgaca gacggaagtc cagcgtaacc accagatcca gcttgccttc
    11401 cagaccattg tcctgccagt ccacttcttc cggcttcacg ccgccctgtt gccccagatc
    11461 tttaccctgg ataccgtgct ccgtccccag caggtacttg agcataaact catgaccttt
    11521 accggaagaa ccgagcaggt tagaacgcca gatgaacagg ttgcgcgggt ggtttttacc
    11581 gttttctggt tgttctgccg caaaacggat ggaaccctct ttcagggatt tcaccgtata
    11641 gtccaccgga ttcatcccgg ctttttccgc ttcgccagcg atagtcagcg ggttagtgcc
    11701 taactgcggt gcagacggca gccagcccat gcgttccgca cggacgttaa agtcgatcaa
    11761 gtgtccggta tagcgggatt tgtccgccat cggtgacagc aactcttccg ccgtgacggt
    11821 ttcataacgc cactggctgg agtggttata gaaataagaa gtgctgttca tgtgacgcgc
    11881 cggacgctgc cagtcaaggg caaacgccag cggctgccag ccggtttgcg gacgcagttt
    11941 ttcctgacct acatagtgcg cccagccgcc cccgctctga ccgacacagc cgcagaaaat
    12001 cagcatgttg atcagaccac gatagttcat atcgaggtga taccagtggt tcagccccgc
    12061 accgacgata atcatcgaac gaccgtgcgt tttatcagcg ttatcggcaa attcacgggc
    12121 gatgcgaata atctggctgc gagaaacgcc ggtaatctgc tcggcccagg ccggggtata
    12181 agctttcaca tcgtcatagc tggttgcaca gttaacgtcg ttcaggccac gttccagacc
    12241 gtagtttgcc agcgtcagat cataaacggt ggtcaccagg gcggtgctgc catcagccag
    12301 ttgcaggcgt ttcaccggca gtttgtgcag cagcacgttt tccagttcca ctttgttgaa
    12361 gtgttccgtg ccgtcgccac caaagtacgg gaagcctacc tcagcgatct catcctggct
    12421 acccagcagg ctcagttgca gctcggtttc ttcgccagtt ttgccgtcgc gctgctcaag
    12481 attccatttg cccttctcgc cccagcggaa gccaatagaa ccgttcggcg caaccatttc
    12541 gccattggta ttaaaggcga cagttttcca ttccggattg ttttcctggc ccagcgcatc
    12601 aaccagatca gcagcgcgca gcatacgacc tgcagcgtag taaccgtcgc gttcttccag
    12661 catcaccagc atcggcatgt cggtgtagcg acgcacatag tcggtgaaat actggcttgg
    12721 gttgtcgagg tggaattcac gcagcattac gtggcccatc gccagcgcca ttgccgcatc
    12781 ggtgccctgt ttcggtgcca gccacagatc gcacagtttg gcgatttcag cgtagtctgg
    12841 tgtgacggca acagttttgg tgcctttgta acgcacttca gtaaagaagt gagcatccgg
    12901 ggtacgcgtc tgcggcacgt ttgaacccca ggcgatgatg tagctggagt tataccagtc
    12961 agcagattcc ggtacgtcag tttgctcgcc ccaggtttgc ggagacgcag gaggcaggtc
    13021 gcagtaccag tcgtagaagc ttaagcaagt accgccaatc agcgagagat agcgtgcacc
    13081 cgatgcgtaa gaaaccatcg acattgccgg aattggcgag aaaccagcaa cacggtccgg
    13141 gccgtagttt ttgatggtgt aaacgttaga tgcggcgatc agttcgttca cctcctgcca
    13201 agaagaacga acaaatccac cgcgtccacg cgcctgctta aagcttttcg ctttatcggc
    13261 gtcttcaatg atagaagccc atgcctcaac cggatcgcta tgcagcgcct tcgcttcacg
    13321 ccacattttc atcaggcgtt tgcgcatcat cgggtatttc aggcggttgg cactgtaaag
    13381 ataccaggag tagctggcac cgcgcgggca gccgcgaggt tcatggtttg gcagatccgg
    13441 acgggtacgc ggatagtcag tctgctgggt ttcccaggtg accagaccgt ttttgacgta
    13501 gattttccag ctgcaggagc cggtgcagtt taccccgtgg gtagagcgga cgattttgtc
    13561 atgctgccaa cgctggcgat atccatcctc ccagtcacgg ttggtattga gaagctggcc
    13621 atgcccatcg gcaaaggttt cacccttctg tttgaagtag cgaaaccggt ccaggaattt
    13681 actcatcggt tttctcctgt gggagcctgt cggccctctg ataaatcgac attgctaatg
    13741 tttaagggca aggtaacgct ctgaaacagc gtgggaattg ataacgatca agagtgatgg
    13801 ggatgaaaaa taaagtaaat tcctaagata cccctttagg ataattaaca gttacagcat
    13861 aacatgctgt aaattaagac gttttatacc tatagttatt taaactatta agatttttat
    13921 gcaaatgggt attaaggagt attccccatg ggtaacatat taatggctct taatgaaatc
    13981 cggcgaaata agccatataa cactaatatg ttgtaactaa atgacgcata aaaaaagcgc
    14041 ggcatcatgc cgcgcaaagg ataatcaaat attaagattt attttttaga atgccgacca
    14101 tataccgccc aagtaatcac tacgcaggcg atatagaaaa tcaaaaatac tttcattgca
    14161 cctactggcg aacccgttaa tgccagcgag ctaccaaacg ctttcgggat aaagaagcca
    14221 ccaatcgcgc caatcgcaga gatgaaaccc agcgccgctg ccgtgccggt tgccgcttca
    14281 cgcatcgcac gttcgtcaga acccccttct gctttcacgc gatccattgt cagtttacgg
    14341 aagatcactg aaatcatctg gaaagtggaa ccactaccca gcccagctgt caggaacagc
    14401 gccaggaaga ccgcgaagaa cgccatgaag cttccgccct gcccgtcagt cggtaaggtc
    14461 aggaacagca ggccgctgaa aatcgccatc agaataaagt tcaccagcgt gacacgagtt
    14521 ccgcccagac ggtcagataa tgcaccacct gcagaacgcg ccagcgcacc aataaacggc
    14581 ccgaagaaag cgtattgcag aatctgaaca tccgggaact gcgtttttga cagcatcgca
    14641 aagcccgcgg agaagccgat gaaggagccg aaggttgcca gatacagcag gctcataatc
    14701 cacagatgac cccgtttgag taccggcaac tgctccttga tggaggcttt cgaggtagca
    14761 agatcgttca tgccaaacca cgccgcaatg gtgaagatgg caaggaacgg cacccatatc
    14821 caggacgcat tcgccagata cagctcagtc ccatccggct gtttgacgcc ctggctacca
    14881 aatactgcga aaatcgacag tgataccacc agcggagcaa ccaactgcat gacgctgacg
    14941 cccatgtttc ccagaccacc attcagaccc agcgcgccac cctgcttctg tttcggaaag
    15001 aagaagctga tgtttgccat actggatgcg aagttcgcgc cagcaaagcc gcatagcaga
    15061 gagatgatga tgaagacgct ataaggcgtg gaggtatcct gcacggcaaa acccagccag
    15121 acgcaaggaa taatcagaat accggtgctg aacgccgtcc agcgacgacc accgaagata
    15181 ggaaccataa aggagtatgg aacacgtaat aacgcgccag aaaccgaagg cagcgcagtc
    15241 aacataaata gctgatcggt cgtaaaatta aaaccgactt tcggtaagtt caccgcaaca
    15301 gcgctgaaca acatccatac gcaaaacgcc agcagcagac agggaacgga aatccacagg
    15361 ttgcggctgg caatacgttg accgcgttgt tgccagaacg caggatcttc cggtcgccaa
    15421 tctgttatga cagctccagt agccctttcg gaggcagatg agtgactcat agacacctct
    15481 gatactcgtt tcgatgtctg ccaccttagt gtctggagct aaaggcaatt tgatgtaaat
    15541 caaacgataa gataacttta tctatcattg atatttatca ttacccatag tgagtacagt
    15601 gatttcataa aaattatgag atttttcacg gtgctgtaga atccctaccc ttaccgatgt
    15661 aaagcgacta accacacggc aaataaggag taactctttc cggctatggg tatacttcag
    15721 ccaatagccg agaatactgc cattccagaa tatatcgtca cattcattaa ggttattgct
    15781 catttaaagc ctgaaggaag gggtttacat gcttaaacgt tgtctctctc cgctcaccct
    15841 ggttaatcag gttgcgctta ttgtgttgct ttctactgct attggactgg cagggatggc
    15901 ggtttctggc tggctggtgc aaggcgttca gggcagcgcc catgcgatca acaaagcggg
    15961 atcgctgcgc atgcaaagtt accgtctgtt ggcggcagtg ccattaagcg agaaagacaa
    16021 gcccttaatt aaagagatgg aacaaacggc atttagcgcc gagttgactc gagcagcaga
    16081 acgagacgga caactggcgc aattacaggg cttacaagat tactggcgta atgaactgat
    16141 ccctgcgctg atgcgtgcac aaaaccgaga aacggtgtca gcggatgtca gccagtttgt
    16201 tgccgggctt gatcagctgg tatctggttt tgaccgcacc acggaaatgc gtattgagac
    16261 agtggtactg gtccatcggg taatggcggt atttatggca cttttactgg tgttcactat
    16321 tatctggttg cgggcgcgac tgctacaacc gtggcggcaa ctgctggcaa tggcgagtgc
    16381 cgtcagccat cgcgatttta cccaacgcgc aaacatcagc gggcgcaacg aaatggcgat
    16441 gcttggaact gcgttgaaca atatgtctgc agaactggcc gaaagttatg ccgtacttga
    16501 gcagcgggtt caggagaaaa ccgccgggct ggagcataaa aatcagatcc tctctttttt
    16561 atggcaggct aaccgccgtt tgcattcccg cgccccgctg tgtgaacgcc tgtcaccggt
    16621 actcaacggc ttacagaatt taaccctgct acgtgatatc gaactgcggg tgtatgacac
    16681 tgatgatgaa gagaatcatc aggagtttac ctgccagcca gatatgactt gtgatgataa
    16741 aggctgccag ctctgcccgc gcggcgtatt acccgttggt gatcgcggca cgaccctgaa
    16801 gtggcggctg gctgactctc atacgcagta cggtattttg ctggcgaccc tgccgcaggg
    16861 gcgtcatctt agccatgacc aacaacaact ggtggatacc ctggttgaac aacttaccgc
    16921 cacgctggcg ctggatcgcc atcaggaacg tcagcaacag ttgatcgtga tggaagagcg
    16981 tgccaccatt gcgcgcgaac tgcatgattc tattgcccaa tctctctctt gcatgaagat
    17041 gcaggtgagt tgtttacaga tgcagggcga tgcgctgcca gaaagcagcc gcgaactgtt
    17101 aagtcagatc cgtaacgaac tgaatgcatc ctgggcgcag ttgcgtgaat tgctcaccac
    17161 attccgcttg cagctcaccg agcctggatt acgtccggcg ctggaagcga gttgcgaaga
    17221 gtacagcgcc aaatttggct tcccggtgaa gctggattat caattgccgc ctcgtctggt
    17281 gccttcacat caggcaatcc acttgttgca aattgcccgt gaggcattaa gtaacgccct
    17341 caaacattcg caagcgagtg aggtcgtggt gacggtggcg caaaacgata atcaggtcaa
    17401 actgaccgtc caggataacg gctgtggcgt gcctgaaaat gccatccgca gcaatcacta
    17461 cggcatgata ataatgcgcg accgtgcgca aagtttacga ggcgattgcc gcgtccgccg
    17521 tcgtgaatca ggtggcaccg aagtggtggt cacctttatt cccgaaaaaa ctttcacaga
    17581 cgtccaagga gatacccatg agtaatcagg aaccggctac tatcctgctg attgacgatc
    17641 acccgatgct gcgaactggc gtaaaacagc ttatcagtat ggcaccagat atcaccgtgg
    17701 ttggcgaagc gagtaatggc gaacagggta ttgaactggc ggaatctctt gatcccgatc
    17761 tgatcctgtt agatctcaat atgcccggca tgaacggtct ggaaacgctg gataaactgc
    17821 gcgaaaagtc cctctcaggg cgcattgtgg tattcagcgt ctctaaccat gaagaagatg
    17881 tggtcaccgc actgaaacgc ggcgcggatg gctatctgtt aaaagatatg gaaccggaag
    17941 atctgctgaa agcattgcat caggcagctg ctggcgaaat ggtattaagc gaagcattaa
    18001 cgcctgttct ggccgccagc ttgcgcgcta accgtgccac tactgagcgc gatgttaacc
    18061 agttaacccc acgcgagcgc gatattctca agctgattgc ccagggtttg ccgaacaaga
    18121 tgattgcccg ccgcctggat atcaccgaaa gcacagtaaa agtgcacgtc aagcacatgc
    18181 tgaagaaaat gaagctcaag tctcgcgtgg aagcagcggt atgggtgcat caggagcgca
    18241 ttttctgatt acggttccca gcgcagttcg tcgtttgaca atgcatcgaa cggttcgaca
    18301 agcgttagcg tgatctcatt ggaggagaca cgctgcccct ggttatcttc caccaccaca
    18361 gacaatcgcc agtgattgct tgccccttcc ccgttctgcc agtcaggcat gatcagcgtc
    18421 cagccctccg cgctgttggc ttgtgcgccc ggcgtcaaac ttaatatctg cgtatcgccc
    18481 tgccaaatca gttgccgaat accgtaacga ctgcggattt gtaatttcag cggcactgtt
    18541 tcgccaggtt ttagatccca cggcggtgtc gccagaaaca ccgttaacgt ttttcgctgt
    18601 cggtactcaa gagtcggtag attatttcgc tgcggattgt catagcgact accacgtaac
    18661 gactgacttt cggcaacctc gcccgcagaa agttgttttt tgagcggtac accaaagcgg
    18721 taattaagat tcagcccgag gttattttga ttttcgccac tttccccctg tttatgctgg
    18781 gccgtcacag tgactaatgg cacaggggtg taatttaatc ccagactcaa cgcgacggga
    18841 ttgtgataac ccgtaccaga gttaaacaaa tcaacacgat caccaaaata ctgttctagg
    18901 ctgacactgg tattgaggtg ttgatagaac ggcatgcgca tccgagctgt caggtcgtac
    18961 ccgcgcgcca tccgttgttc ctgcgtggct gtctgttcat gccatgcagc aaacggctga
    19021 taaaagtttg ccgataatcg caaatattcg ccccacgctt cggcaccaaa gcccgctcgc
    19081 tgaagatttt cgtcctgcaa gttgtcataa aaagtgttat aacccaccag ccagttgccg
    19141 cgcgcccagc gttgcccaac gcccacattg ctcaccaacc cattatcctg ctgagtaaga
    19201 ccaagctggc tccaggtgag ataacgatca ttatcttgta acggcacaaa ccagcttcca
    19261 cgactgccgg tgaaatgtcc ttcgttatcc actttgacgt caacactggc atttccccac
    19321 ggtgatagcc aggactctac gtgctgatta acctgttgac taagcgcgtc gcggactttt
    19381 cccaatgcga aagcttttgc ctgctcgcca gtatccagcc cgttatcatt catactggtt
    19441 tcgccgaaat ctttcacaat ttcagcaaag tgtttttccc catcatgatt ttcgggagcc
    19501 atgcctaaat ccggcagacc atcattgtta ttatcaaagg gatttgccgc tttttgctcg
    19561 aaggtagatt gtgcgttagc tgtaccgcct gctacaagca agagtaaata aaacgggata
    19621 atgcgaggaa cgaaacggct caaaacgtcg gtaacttccg gtaataatga aaaaccatag
    19681 ccttaaagaa taacgattac tggcaacttt tgtcgtattt tctcgcgctt tcaggaaaat
    19741 ccttaaaaat gtgaccgatt ggtcagacgc tgcagaagca gtaagataga agatgagtgt
    19801 ctaattccct gaatacagga gtaaataatg caaaaaatag tgatcgttgc caatggcgca
    19861 ccttacggga gcgaatcctt gtttaacagc ttgcggctgg ccattgcgtt acgagagcag
    19921 gagagcaatc tggatctgcg tctgttcctg atgtctgatg cggtcacagc cgggttgcgc
    19981 gggcaaaaac caggggaagg ctacaacatt cagcaaatgc tggagatcct taccgctcag
    20041 aatgtaccgg tgaaattgtg caaaacctgt accgacgggc gcgggattag cacacttcct
    20101 ctgattgatg gggtggaaat cggtactctt gtggaactgg cgcaatggac gctgtcagcc
    20161 gataaagtgc tcacatttta atgtttaagg gggcgtctga cgccccacta tgagggaagt
    20221 ttactcaggc gaatcccggc cttaacacac catccggata attctccgcc agtagttttt
    20281 ttaccgatac cagcaaatca ttcagcccat cgtcctgcat tcccagtttg ataagctcct
    20341 gttccagtga aaacaggtat tgtgcattgg ttcccagcgg accgctcgcc gccgcaatca
    20401 acggcgcgat gacctgagcg cgagtgtcag attcatattc tggatgtcgc gggtccataa
    20461 taaacacaat ggcgtttact gtgcgtccat catcaagatc aagctgacac caggttggca
    20521 gataacagcc ggtaatcatc tcgcgcttcc acaacagggt tagttcctgc tccagcgtct
    20581 cttctggtag tcgataggcg acgcctgtgg tgcgtccgcc ctctttcagt gcaagcatcc
    20641 gtcccggctg gtgcgcagtc ccgcgcccgg cggtcaggcg caggcaaaac gcgcgatgcc
    20701 atccaaccag tgtaccggtg catgattcgg taaactccag tgccggattc cacatcaacg
    20761 atccataacc gaagatccac accggtccct catcaggtcg acaagccagc gtcgccgcca
    20821 gcgaagccgc ccgttgttct gctgaccata agagtgattc ttcaatggca ccaaatgccg
    20881 ttttacaatc ggcattcatc aagaaatcac gcgttatcac cttacacctc cgccactgca
    20941 tacttccctg cgacaacttt ttggtgtctt ccggacattt ttaccgctca ttttgaaacc
    21001 actaacggaa caatatgcaa tccacggacc acttgcaagg caacaccgca cgaatagctt
    21061 taactaagct accggtttta cgatttttta tgccatttat catcatcccc tttggcatat
    21121 tcatgcttca cagcagccca ggccacttta tgcgcggttt cttcgcgact ggcgtcatca
    21181 cgccgatctt ctttatcttt atattgatcc catgcgctgt tgaacgcttc tttatagata
    21241 tcctgggcat gagacggtag aacgtgcttt acgctttccg gcagatcgct tttcgtttta
    21301 tacggcatcg ttgactcctt ttacaaagtt gaacattaag tgtggtcaac aatatctact
    21361 cttgccaaaa cataagaatt ttcatgtcag actgttctta agaaacgcta aaacaaacat
    21421 tgtctaaacg taatacttat ttttatgccc caatgagtaa aaaatcgaca gcggggtaaa
    21481 aattcgtaaa atgttaccga tatccagaca ctttctgtta tttttcctac ctatttttta
    21541 cattttcgat aaattcatct gcaaagggat aaccataatg tcaaatgctc aagaggcggt
    21601 aaaaacccgc cacaaggaga cttcgcttat tttcccggtt ctggtgctgg tagtgctgtt
    21661 cctgtgggga agtagccaga cactaccagt ggtcattgcc atcaatcttc ttgcgcttat
    21721 tggtatttta agtagcgcct ttagtgttgt ccgtcatgcg gacgtattag cccatcgcct
    21781 gggagaacct tacggttcgc ttattcttag cctttcagtg gttattcttg aagtcagttt
    21841 gatttcagct ttaatggcaa ccggcgacgc cgcgccaacg ctaatgcgtg atacgctcta
    21901 ttcaatcatt atgattgtta ccggtgggct ggttggcttt tcattattgt tgggcggtcg
    21961 taagtttgcc acccaatata tgaatctgtt tggtatcaag cagtatttaa ttgcgctgtt
    22021 ccccctggcg ataatcgtac tggtatttcc aatggctctg cctgcggcga atttttcaac
    22081 cggtcaggcg ttactggtag cattaatttc tgcggcaatg tatggcgtat ttttgctgat
    22141 ccaggccaaa acgcatcaaa gtttgtttgt ctacgagcac gaagatgaca gtgatgacga
    22201 cgacccgcac cacggtaaaa cgtctgccca tagcagcctg tggcatgcta tctggttgat
    22261 tatccatctg attgccgtta ttgcagtcac caaaatgaac gccagctcgc tggagacatt
    22321 gctcgacagc atgaatgccc ctgttgcctt tactggcttc ctggtggcac tgttgattct
    22381 gtcgccggaa ggtttaggtg cgttaaaagc agtgttgaac aaccaggttc agcgcgcgat
    22441 gaatctgttc tttggttcag tgttagcaac aatttcgctt accgtacctg tcgtcacgct
    22501 aattgccttt atgacgggta acgaattgca gtttgcactt ggtgcgccag aaatggtggt
    22561 gatggtggcc tctttagtgc tgtgccatat ctccttctcc accggacgta ctaacgtgct
    22621 caatggcgca gcgcatctgg cactgtttgc cgcctatttg atgacgatat ttgcctgaca
    22681 gttatatctc ctccgatgac ggaaatcggg agagataaga taaattcctg cttattgtat
    22741 ttcttcatac tacctggaca gctcgttaat cagaattgcg agcctgtacc acaacctgca
    22801 cgaaaccact gcaacagctg ttgaaaagcc ggaatgtact ctttattgca aacatttcag
    22861 ggatgaagtg aagatgaatt gagacgtagt atccacacca agccggaatt atccggtgag
    22921 gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggaaaga gaaaaccccc
    22981 gcacgttgag aggtaaaacc tgacaacaaa acgggggcta atcttgaaac cagacacttc
    23041 aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct cgcgcagttt
    23101 gccatgactt tctggcacga cctggcagca ccgatcctgg cggggattat tgccgcagcg
    23161 attgtcagct ggtggcgtaa ccggaagtaa ggtgaaaacg ggcgtcaggc tgccgtgatg
    23221 gcaatatgcg cccgaaaccg gccgcagggg taacagcctg cggccttttt tgtatctggc
    23281 acccatgaaa aaagtcttaa cgcagaacgc taagacttta tttttcaagc aaaaaagatt
    23341 acttgctggt atccagttct tcgaaacctt tcaccagatc atcaatcgct ttcatctgct
    23401 tgaggaacgg ttccagttta gccagcggca gcgcggatgg accatcacat ttcgcatgtt
    23461 ccggatccgg atgcgcttca ataaacagcc ccgccagacc taccgccata ccggctcgtg
    23521 ccagctcagc cacctgagca cgacgaccac cggaagctgc gccaaacgga tcgcggcatt
    23581 gcagtgcgtg ggtcacgtcg aaaatcaccg gcgagttacc agacactttc ttcataatgc
    23641 tgaagcccag catatcgaca accaggttgt catagccgaa gttagcacca cgatcgcaaa
    23701 gaatcacttt ttcgttgccg ccttctttga atttatcaac gatattaccc atctgtcccg
    23761 ggctgacaaa ctgtggtttc ttgacgttaa ttaccgcacc ggttttcgcc atggcttcaa
    23821 ccaggtcagt ctggcgagca agaaacgccg gcaactgaat cacatccacg acatcagcaa
    23881 cgggctgtgc ctgacttggt tcgtgaacgt cggtgataat tttcacgcca aaagtctgct
    23941 tcaactcctg gaagattttc atcccttctt ccaggcccgg tccacgataa gagtggatgg
    24001 aggagcggtt ggctttgtca aaagaggctt tgaacacgta agggataccc agtttctggg
    24061 tcacagttac gtagtgctcg caaatgcgca tcgccagatc gcgagattcc agcacgttca
    24121 taccgccaaa cagtacgaac ggcaggtcat ttgctacgtt gatgtcgcca atgctaacca
    24181 ctttttgttt cataggatcg ccttaatctt gagtaaaatg tcgattaatt aatgcagcac
    24241 aatatgttta tgcgcgatgt tatttatttg cgcacggatc atttcgctga tcgggtcttc
    24301 cggacaccgt tcaacgaaat aacttaaatc gttcaacgca acgtgttcgc aatccagttg
    24361 cgcataaatc aacccacgat cgcgaatttc atagggatct tcagggttga attgtaataa
    24421 agcttcgctg gtgcgtaacg ccagctccat ctgattttct tccatcaacg aggctttgag
    24481 tgtatccagc aatttgcgga ttacctcaat gttatcagct tcatcaaggt cttcataaaa
    24541 cagttccgcc gacgggctga tatttccctt taaccatccg tccagcatat gttcgcttaa
    24601 cgattcaccg ttaaaaggat taatcagcca aatttcgcca tccggacatt caatgcgcaa
    24661 tatcagctgc gtagggaaaa tcaccggcag cagcggcaaa tcgagacgat tcgcgaccca
    24721 taataaaacc gcacctaatg ataccgcact gccctgtcga ttctttaaca cctggtccag
    24781 ccataatgca tcggaaagac gataaacgcc gcgtgaggct ttaaatcccc agtcgccgta
    24841 aaacagcgcg atcagttttt ccagctgctc ttctaaaggc agaagctggc tgatttcttc
    24901 cttcgccaga ctaacgagac gctccagttc gtcgtaaaca tcttgcgagg gaaaatcgcg
    24961 gcggattgct tcgcaagcca ggatcatgcc ttcgcacaat ggcgctttat taaattcgaa
    25021 atcagctaac gatctcatga cttaccccag taacggtact tttgtggtgg cgagtttaat
    25081 gatgatgtac agcaccacca acgccagcgg gaaggcaata atgcgcgcct gctgactgtg
    25141 catacgacga taatcgagtg caataaaacc caaaacgata taaatgataa ctccaaacag
    25201 cttttcagtc agccactgtg cctgcccgga aaatggcagg atgtgcgctt tagccatcaa
    25261 cgcaatgccg cttaacaata acaccgtatc gacaaccggc ggcacaatgc gcgtccagcg
    25321 agcaaatgcc tgtggatgct tctgataacg tagccagaaa cgtaaggtta atagcccaac
    25381 agaaagcgcg atactaataa gatgaacact aagcagtgta gaaaaacttg tcattgataa
    25441 tagcggccga gcgttacgcg ctcgttatca ccatagtcac ggcaggtttc gacgtcatga
    25501 taccccgcga ggataaatgc ttgtcgcacc gcttcgccct gctgccagcc atgttccaga
    25561 agcagaaagc cgccggatac cagcgcgtta cgcgactgtt cgatgatatg cacgatgtct
    25621 gccattccac tgtctgccgc aaccagcgca gtgagcggct caaagcggac atcgccttgt
    25681 tgaagatgtg ggtcctgctc gtcaatatac ggcggattgc tgacaatcat cgcaaactgc
    25741 tgcccggcta gcgcgctaaa ccagtcgctt tgcagaatgt ggatattttt gatcgccaga
    25801 tgctgggcat tacgttgtgc cagggagaca gcatcaggca tacgatctac agcgataatt
    25861 tcgcagtccg ggcgctcgct agccagcgca agcgcaatcg ccccggtacc cgtcccgaga
    25921 tcgagaatac ggcaaggttg ttcaggcaac cgcgccagtg cctgctccac cagacactcc
    25981 gtatccgggc gcggaattaa ggtcgctggc gaaacaaata acggcaacga ccagaattct
    26041 cgcaccccgg ttaaatgagc aatgggttca ccatcgcgac gacgtgtcag tagcgcatca
    26101 agttgctgac attgttcgtc agtcagctgc gtttcaccaa aggcgagaat aaaagtacgc
    26161 cctctgccgg taacatgctc cagcaggatt tcagcatcac gccgcgggct ttcgctcgcc
    26221 tgaagttggc ttattgcttc acgtaaccag tgttgatatt ccattattcc tgctcggaca
    26281 acgccgccag ttggtcggcc tgatgttcct ggataatcgg ttcaatcagc atatccagct
    26341 taccttccat cacttcatcc aggcggtaga gcgtcaggtt gatgcggtga tcggtaacgc
    26401 gcccctgcgg gaaattgtaa gtacggttac ggtcgctgcg atcgccactc cccagcaggt
    26461 tacgacgggt agacgcttcg gcctgttggc gttttgccat ttcagcagcg tggatgcgag
    26521 caccgagcac tgaaagcgcc ttcgctttgt ttttatgttg tgaacgttcg tcctgacatt
    26581 caacaacaat cccggtcggc aagtgagtaa tacgaattgc cgaatcggtg gtgttaacgt
    26641 gctgaccacc cgcccctgac gagcggaaag tatcaatgcg taaatctgct gggttgatgt
    26701 ccggcagttc tgcgtcaggc agttctggca ttaccgcaac ggtacaagca gaagtatgaa
    26761 tacgaccctg cgattccgta gcaggaacac gttgcacgcg atgaccgccg gattcaaatt
    26821 tcagacgacc atacacacca tcaccgctaa ttttggcgat gatctcttta taaccaccat
    26881 gttcaccctc gctggcgctc atgatttcta cccgccagcg gcgggcttcg gcgtaacggc
    26941 tgtacatacg gaacagatcg cctgcaaaca gcgctgcttc gtcgccgccg gttccggcac
    27001 ggacttcgag gaaggcgtta cgttcgtcat caggatcttt tggtagtaat agaacctgta
    27061 attgctgttc cagttgctcg cttttttctt tagcttcgcg cagttcatcc tgcgccatct
    27121 cacgcatttc aggatcgtcg agcatcatct gtgcggtttc gatatcttcc tgaacctgtt
    27181 gccagtcggt aaaacagcgc gaaacatcac ttaactgcgc atattcgcgt gataatgcgc
    27241 gaaaacgttc ctggtcggcg atagtttgcg cgtcacccag caacgcctga acttcttcat
    27301 ggcgttcatg cagggcttcc agtttggcaa cgatagaagg cttcataggc gtaaatgcac
    27361 cctgtaaaaa aagaaaatga tgtactgcta ctccagcccg aggctgtcgc gcagaatatt
    27421 caggcgttcg ttatccccgt cacgggcggc ctgttgaagt gatttcgttg gcgcatggat
    27481 caagcggtta gtcagtttcc atgccagatc ctgcataatg gcttgcgcgt cgccgccctg
    27541 ctcaagggcc gctaacgctt tggcggttaa ctcatcgcga acgtgctctg cctggctgcg
    27601 atactcgcga atggtttcgc tggcgctttg tgctcgcagc cacgccataa attcgctggt
    27661 ttcctgagcg acaatagttt ccgcctcaac cgctgcggct ttacgctgcg ccaggttgtg
    27721 cgaaatgatg ctttgcagat cgtccacact ataaagataa gcattcgcca gtttgccaac
    27781 ttccggctca acatcgcgcg gaacggcaat atccaccaac agcattggtt ggttacgacg
    27841 actttttaat gcgcgctcca ccatgccttt cccgataatc ggtaacgggc tggcggtgga
    27901 actgatgatg atatcggctt cgcgcagacg ttcgtcgatc tcactcaggg caatcacttc
    27961 cgcgccaact tcatctgcca gaatttgggc acgttcgcga gtgcggttgg cgataatcat
    28021 cttctgtact ttatgttcgc gcagatgacg cgctaccagc tcgatggttt cgcccgcgcc
    28081 taccagcaac actgtgaccg tagagagcga ttcaaagatc tgccgcgcca gcgtacaagc
    28141 cgcaaaagcg acagacacag cactggcacc gatatctgtt tcagtgcgaa cgcgtttcgc
    28201 tacagagaaa gatttctgga acatgcgttc cagttcgctg gccttcatat gacctttttg
    28261 cgaatcgaca aacgcttttt taacctgacc gaggatctgc ggctccccaa gaaccagtga
    28321 atccaggccg ctggcaacac gcattaaatg gctaaccgcg tcgttatcct gatgccagta
    28381 gaggctttta cgcagatctt cttcattaag attgtgataa tcgcaaagcc agcggattaa
    28441 cgcctcttgc aggttatcct gctcttcaac gctaagataa agttccgtgc ggttgcacgt
    28501 cgacagcacc acgccgccct gcaccatcgg ctgcgcaagc aggctgtcaa gcgcctgatc
    28561 gagcttatcc ggcgaaaacg atacacgttc tcgcagcgat acaggtgccg ttttatggtt
    28621 gataccgagt gctaaaaggg tcatgtctgc gggaaataat accaacgttg atagggttag
    28681 tctgcttgca tcatacagga tgcgtaagat caataaaaga gagcgccccc ttttggagta
    28741 attgccgaaa gccgttagat tctggcaatt aagacaactt gaacatagac gatagcggac
    28801 ggtaacgcta gcattaaggg ttataactgc aacgtatctc aaggacttgt catcactatg
    28861 cccctgcccg attttcgtct tatccgcctg ctaccgctgg ctgctcttgt gctcactgcc
    28921 tgttccgtta ccacgcccaa aggtcctggc aaaagcccgg attcgccaca atggcgtcag
    28981 catcagcaag acgtgcgcaa tcttaatcag tatcagactc gcggcgcgtt cgcttatatt
    29041 tctgaccaac aaaaagtgta cgcccgcttt ttctggcagc aaaccggcca ggatcgctac
    29101 cgtctgctgc tcactaaccc attgggcagc acggaactgg agctgaatgc tcaaccgggt
    29161 aacgtgcagt tagtcgacaa taaaggtcag cgttataccg ccgatgacgc cgaagagatg
    29221 attggcaaat tgaccggaat gccaattccg ctcaacagct tgcgccagtg gattttaggt
    29281 ttaccgggtg atgcaaccga ctacaaactg gacgaccagt accgcctgag cgaaattacc
    29341 tacagccaga atggcaaaaa ctggaaggtt gtttatggtg gttatgacac caaaacgcaa
    29401 cctgcgatgc cagccaatat ggaactcacc gacggtggtc aacgcatcaa gttaaaaatg
    29461 gataactgga tagtgaaata atgcggacac agtggccctc tccggcaaaa cttaatctgt
    29521 ttttatacat taccggtcag cgtgcggatg gttaccacac gctgcaaacg ctgtttcagt
    29581 ttcttgatta cggcgacacc atcagcattg agcttcgtga cgatggggat attcgtctgt
    29641 taacgcccgt tgaaggcgtg gaacatgaag ataacctgat cgttcgcgca gcgcggttgt
    29701 tgatgaaaac tgcggcagac agcgggcgtc ttccgacggg aagcggtgcg aatatcagca
    29761 ttgacaagcg tttgccgatg ggcggcggtc tcggcggtgg ttcatccaat gccgcgacgg
    29821 tcctggtggc attaaatcat ctctggcaat gcgggctaag catggatgag ctggcggaaa
    29881 tggggctgac gctgggcgca gatgttcctg tctttgttcg ggggcatgcc gcgtttgccg
    29941 aaggcgttgg tgaaatacta acgccggtgg atccgccaga gaagtggtat ctggtggcgc
    30001 accctggtgt aagtattccg actccggtga tttttaaaga tcctgaactc ccgcgcaata
    30061 cgccaaaaag gtcaatagaa acgttgctaa aatgtgaatt cagcaatgat tgcgaggtta
    30121 tcgcaagaaa acgttttcgc gaggttgatg cggtgctttc ctggctgtta gaatacgccc
    30181 cgtcacgcct gactgggaca ggggcctgtg tctttgctga atttgataca gagtctgaag
    30241 cccgccaggt gctagagcaa gccccggaat ggctcaatgg ctttgtggcg aaaggcgtta
    30301 atctttcccc attgcacaga gccatgcttt aagccgggca agctgagttt cggtgacaac
    30361 gtcaccttgt tccagacgtt gcatcgcgct ctttaataca ccgcctggaa aggatcatgc
    30421 ctggcccgca cagttttcgg cagattcttt ccaccaatgg acgcatgcct gaggttcttc
    30481 tcgtgcctga tatgaagctt tttgctggta acgccacccc ggtactagca caacgtattg
    30541 ccaaccgcct gtacacttca ctcggcgacg ccgctgtagg tcgctttagc gacggcgaag
    30601 tcagcgtaca aattaacgaa aatgtacgcg gtggtgatat tttcatcatc cagtccactt
    30661 gtgcccctac taacgacaac ctgatggaat tagtcgttat ggttgatgcc ctgcgtcgtg
    30721 cttccgcagg tcgtatcacc gctgttatcc cctactttgg ctatgcgcgc caggaccgtc
    30781 gcgtccgttc cgctcgtgta ccaatcactg cgaaagtggt tgcagacttc ctctccagcg
    30841 tcggtgttga ccgtgtgctg acagtggatc tgcacgctga acagattcag ggtttcttcg
    30901 acgttccggt tgataacgta tttggtagcc cgatcctgct ggaagacatg ctgcagctga
    30961 atctggataa cccaattgtg gtttctccgg acatcggcgg cgttgtgcgt gcccgcgcta
    31021 tcgctaagct gctgaacgat accgatatgg caatcatcga caaacgtcgt ccgcgtgcga
    31081 acgtttccca ggtgatgcat atcatcggtg acgttgcagg tcgtgactgc gtactggtcg
    31141 atgatatgat cgacactggc ggtacgctgt gtaaagctgc tgaagcgctg aaagaacgtg
    31201 gtgctaaacg tgtatttgcg tacgcgactc acccgatctt ctctggcaac gcggcgaaca
    31261 acctgcgtaa ctctgtaatt gatgaagtcg ttgtctgcga taccattccg ctgagcgatg
    31321 aaatcaaatc actgccgaac gtgcgtactc tgaccctgtc aggtatgctg gccgaagcga
    31381 ttcgtcgtat cagcaacgaa gaatcgatct ctgccatgtt cgaacactaa tcgaacccgg
    31441 ctcaaagacc cgctgcggcg ggtttttttg tctgtaatat ccatttgtat gacctatgcc
    31501 tccttcacct gccatttagt tgacagatga tgcgctcacg gatgaaacat tattgtgaac
    31561 aaaatatttt cctcacatgt gatgcctttc cgcgctctga tcgacgtttg ctggaaagaa
    31621 aaatatactg ccgcacggtt tacccgtgac ctgattgccg ggataaccgt cgggattatt
    31681 gctatcccgc tggcgatggc gttggctatt ggtagtggtg tggcccccca gtacggttta
    31741 tataccgcag ctgttgcggg gattgtcatt gctctaacgg gtgggtcacg ctttagcgtt
    31801 tccggtccga ctgcggcatt tgtggtaatt ctctatcccg tttcgcaaca gtttggactg
    31861 gcaggactgc tggttgcgac cttgttgtcg ggaatctttt tgattctgat ggggctggca
    31921 cgctttggtc gtctgattga gtatattccg gtttccgtca ccttaggttt cacctcgggt
    31981 atcgggatca ccatcggtac catgcagatt aaagattttc tcggtctgca aatggcccat
    32041 gtcccggaac attatctaca aaaagtcggc gcattattta tggcgctgcc gaccattaat
    32101 gtgggtgatg ctgccattgg cattgtgacg cttggcattc tggttttctg gccgcgtctg
    32161 ggcattcgtt tacccggtca ccttccggcc ttgctggctg gttgcgcggt gatggggatt
    32221 gttaacctgc tcggcggaca tgttgctacc atcggttcgc aattccacta cgtcctggcc
    32281 gatggttctc agggtaacgg tattccgcaa ctgctgccgc aactggtgct gccgtgggat
    32341 ctgcctaatt cagaattcac gctaacctgg gattctattc gcaccctgct gcctgcggca
    32401 ttctcaatgg caatgctcgg ggcgatcgaa tctctgctct gcgctgtggt actggatggt
    32461 atgaccggga ctaagcacaa ggcgaatagt gaactggtcg gtcaggggct ggggaatatc
    32521 atcgctccat tctttggtgg tattaccgct actgctgcca tcgcgcgttc tgccgctaac
    32581 gtccgtgccg gggcaacttc ccctatctcg gcggtgatcc actctattct ggttattctc
    32641 gccctgctgg tactggcacc actgctctcc tggctgccgc tttccgccat ggcagccctg
    32701 ctgttgatgg tggcgtggaa catgagtgaa gcgcacaaag tggtcgactt gctgcgtcat
    32761 gcgccgaaag atgacatcat cgtcatgctg ctgtgtatgt cactgaccgt gctgtttgat
    32821 atggttattg ccatcagcgt ggggatcgtg ctggcatcgc tgctgtttat gcgtcgtatc
    32881 gcacgtatga ctcgcctggc accggtagtc gtagatgttc cagacgatgt cctggttctg
    32941 cgcgttattg gcccgctgtt ttttgctgct gctgaaggct tattcacgga cctggagtca
    33001 cgtcttgaag gcaaacggat tgtgattctg aagtgggatg ccgttccggt acttgatgct
    33061 ggtggtcttg atgcgttcca gcgttttgtg aagcgtctgc ccgaaggatg tgaactgcgc
    33121 gtgtgcaacg tggaattcca gccactgcga actatggctc gcgcaggcat tcaaccgatc
    33181 ccgggacgcc tcgcgttctt cccgaatcgt cgcgcggcga tggcggattt ataagtacga
    33241 gattgaccag tcagcgcaaa ctgactggtc agcaaactgc attatttgtt agctatgacg
    33301 gcgattatcg ctacggcggc aacgtttgtc atagtgacga acccaccagt aacggtcgca
    33361 cacctgttca tgcccgccaa cacgggcacc cgccagccag agaatggcac cgacgaaaat
    33421 acttagcact gcaccatgtg cgaaatactg gggcatatta aactgtggta actggttgag
    33481 gattgaatac cccacgccga ccaccattac caccagaccc aaccccatga gcacgttacc
    33541 gagtatcgaa gcgtttttgc gtttcatatg tcacctccgg aactttctgg gttgtaacag
    33601 ggaatacccc tcttccttat gtgtaaagta tagacaacac cctgtgggtt atgtgcgggc
    33661 gtaatcacaa ttacaaccct tatttcaaca aaactttaca aataaacgcc tgaacacctt
    33721 gactttctaa tagtccactc aggcaattat tacgttatct gcatttgata cgcagttttt
    33781 tgtcaagcgg ggccgcaacc agtaaactac gcgccagtta tgtacacact caggacaaaa
    33841 aaacgtgacg attaaattga ttgtcggcct ggcgaacccc ggtgctgaat acgccgcaac
    33901 gcgacataat gctggtgcct ggttcgttga cttactggca gagcgtttgc gcgctccgct
    33961 gcgcgaagag gctaaattct ttggttatac ttcgcgagtc acccttggaa gcgaagatgt
    34021 ccgcctgtta gtcccgacta catttatgaa tctcagcggc aaagccgttg cggcgatggc
    34081 cagttttttc cgcattaatc cggacgaaat tctggtggcc cacgacgaac tggatctgcc
    34141 tcctggcgtc gccaaattta agttgggcgg tggccatggt ggtcacaatg gactgaaaga
    34201 catcatcagt aaattgggta ataaccctaa ctttcaccgt ttacgcatcg gaatcggtca
    34261 tccgggcgat aaaaataaag ttgtcggttt tgtgttaggc aaaccgcctg ttagtgaaca
    34321 gaagttaatt gatgaagcca tcgacgaagc ggcgcgttgt actgaaatgt ggtttacaga
    34381 tggcttgacc aaagcaacga accgattgca cgcctttaaa gcgcaataag tcgttgtctg
    34441 cggcattttt gccgagtgcc gtgtataata ggcaaagtta tttccatttc tgcaatctgt
    34501 tagcaataac aggttgatta ttaagatatt aaggtgattt aaatcatggg attcaaatgc
    34561 ggtatcgtcg gtttgcccaa cgtcgggaaa tctaccctgt tcaacgcgct gaccaaagcc
    34621 ggtattgaag cggccaactt tccattctgc accattgagc cgaacacagg cgtcgtacca
    34681 atgcctgacc ctcgcctgga tcaactggct gaaatcgtaa aaccgcaacg tacgcttccc
    34741 acgaccatgg aatttgtcga tatcgccggt ctggtaaaag gcgcatcgaa aggcgaaggt
    34801 ctgggtaacc agttcctgac caacatccgt gaaaccgaag cgatcggtca cgttgttcgc
    34861 tgctttgaaa atgacaacat cattcacgtt tccggcaaag ttaacccggc tgacgatatt
    34921 gaagttatca acaccgaact ggcgctggca gacctcgaca cctgcgaacg tgcgattcat
    34981 cgcgtacaga agaaagccaa aggtggcgat aaagacgcga aagctgagct ggcggtcctg
    35041 gaaaaatgcc tgccccagct ggaaaacgca ggtatgctgc gcgcgctgga tttaagcgct
    35101 gaagagaaag cggctattcg ttacctgagc ttcctgacgc tgaaaccaac aatgtacata
    35161 gccaacgtca acgaagatgg ttttgaaaac aacccatatc ttgaccaggt gcgtgaaatc
    35221 gcggcgaaag aaggttctgt tgtggttccg gtttgtgctg ctgttgaagc agacattgcc
    35281 gaactggacg acgaagaacg tgacgagttt atgcaggagc ttgggctgga agaaccgggc
    35341 ctgaaccgtg tgatccgtgc cggttataag ctgctgaacc tgcaaactta cttcaccgct
    35401 ggggtgaaag aagtgcgtgc atggaccatt ccggttggag caaccgcgcc gcaggcagcg
    35461 ggcaaaatcc atactgattt tgaaaaaggc tttatccgtg cacaaaccat ctcgtttgaa
    35521 gatttcatca cttacaaagg tgaacaaggc gcgaaagaag caggcaaaat gcgtgcagaa
    35581 ggtaaagatt acatcgtgaa agatggcgat gtgatgaact tccttttcaa cgtctaatta
    35641 atttattgtc tcatgaggtt tcgctaaatc tcatgaagag cgcaaaagta tataaaatcc
    35701 acgcaattgc gtggattttt catttcaccg agtcctaatc attcttgtaa catcctacac
    35761 ttttgtgctt attgcctaca gtgaaccacg agaataatta aagacataag tgttcagatg
    35821 actgatatct cattagcagt gatataacgt cagtttttgt tccactacac atcagccctc
    35881 ttcacagctg atcaatattg atctcgctcc aatatgctgg cgagaaaagg acttacctga
    35941 agggtaatat cccgcagata aagtccccga ataacgtgat tacgtgcgat cctggcaaga
    36001 tgaaaatgat aaccagtagc acaataataa cgataggcca gaaaatccat tgaacaaaaa
    36061 tgaatatcga tgttttttgc tttttcttat tagtaatgca aaaatggcgc atattgtttt
    36121 tgttatgcag atacatcaag cgaaaatccc ataactaatt aggattatta caaaaaatct
    36181 tcaccaaatt ttttaaccgc tgtttgttgt aatatttttg ccatattcat agtaacgaat
    36241 tggaataagt aacgacaact tagggaataa ctcgatataa actcaccttt gtaagggggg
    36301 ggttaaatat atattaattc ctgtgttcct gattttgccc ggtaatagag aattattctc
    36361 agagaagctc atcatgattg caccatacag atatcatgat gataagagga tattattcat
    36421 tgacttaaac aaaatgacaa aatccataga cccatagcct gggtactata taggagtggt
    36481 atgggcatca aacaacacaa tgggaatacc aaagccgatc gtctcgctga attaaaaatc
    36541 cgttcgccct caattcaact gataaaattt ggcgctattg gtttgaatgc aattatcttt
    36601 tcccccctgc tgatagctgc tgatacagga agtcaatatg gcaccaatat tactattaat
    36661 gatggtgaca gaattacagg agataccgcc gatccatcag gaaacctcta tggtgtaatg
    36721 accccagcag gaaacacgcc tggcaatatc aacctgggta atgatgtcac cgtcaatgtc
    36781 aacgacgcct ctggatatgc aaaaggaatc attattcagg gcaaaaacag ctccctgaca
    36841 gctaaccgac tcacagtaga tgttgttggt caaacctctg ccatcggcat taacttaatt
    36901 ggtgactata cccatgctga cttaggcaca ggcagcacca ttaagagtaa cgatgacggc
    36961 atcattattg ggcatagctc aacactaaca gccactcaat tcaccattga aaactcgaac
    37021 ggtataggcc taaccatcaa tgactatggc accagtgtcg atcttggaag cggaagtaaa
    37081 atcaagaccg atggaagtac aggtgtttat atcggtggtc tcaacggcaa taacgccaat
    37141 ggtgctgcgc gttttacggc gacagacctg acaatcgatg ttcagggcta cagcgccatg
    37201 gggataaacg tacagaaaaa ctctgttgtc gatctcggaa caaacagtac cattaaaacc
    37261 aatggcgata atgctcacgg cctctggagc tttggccagg ttagcgcgaa tgcactcact
    37321 gttgatgtaa ctggagccgc ggccaatggc gtcgaagttc gtggtggtac aaccactatc
    37381 ggtgcagata gccatatttc ttccgcgcag ggcggtggcc tcgtcaccag tagttcagac
    37441 gcgacaatca atttttctgg cacggcagcg caacgaaaca gcatcttttc cggcggttct
    37501 tatggtgcct cggcccagac ggcaacggct gttatcaaca tgcaaaatac cgatattacg
    37561 gttgatcgta atggcagtct ggcgctgggt ttgtgggcgc tcagcggcgg tagaataacc
    37621 ggagacagtt tggctatcac cggcgcggca ggagccagag ggatttatgc catgaccaac
    37681 agccagatcg acctcacgag cgatctggtc attgatatga gtacacccga ccagatggcc
    37741 atcgcaacgc aacatgacga tggttatgcc gccagccgca tcaacgcctc gggtcgtatg
    37801 cttatcaacg gtagcgttct ttccaaaggt gggctaatca atctggatat gcaccctggg
    37861 tcggtttgga caggttcctc cctcagcgat aatgtcaatg gcgggaaact ggacgttgca
    37921 atgaataaca gcgtctggaa cgtaacaagt aattctaatc tcgacacgct ggcgctgagc
    37981 cattcaactg tcgattttgc cagccacggg tcaactgccg gcacatttac cacattaaac
    38041 gtagagaacc tgagcggtaa cagtaccttt attatgcgtg ctgatgttgt tggcgagggt
    38101 aatggcgtta ataatagagg ggatttattg aatatcagcg ggagtagtgc tggtaatcac
    38161 gtattggcta tccgcaacca gggcagcgag gccacaacgg gaaatgaagt tctgacagtg
    38221 gtaaaaacca ctgacggcgc ggcctcgttc agcgcgtctt ctcaggttga gttgggggga
    38281 tatctgtacg atgtgcgtaa aaatggcact aactgggagc tttacgcttc cgggacagtt
    38341 ccggaaccga ctcctaatcc tgaacccaca ccagctcccg ctcagcctcc catagtcaac
    38401 cccgatccta cgcctgaacc cgctcccacg cctaaaccca ccacgaccgc agatgctggc
    38461 ggcaattatc tcaatgtcgg ttacttattg aactatgttg aaaaccgtac gctgatgcaa
    38521 cggatgggtg acttgcgaaa tcagagtaaa gacggtaata tctggttgcg cagttatggg
    38581 ggaagcctgg actcctttgc cagtggcaaa ctgagcggct ttgacatggg ttacagcggt
    38641 atccagtttg gtggggataa acgtctctct gatgtaatgc cgttgtatgt cggtctgtat
    38701 attgactcaa cacatgcatc gccggactat agcggaggcg acggtaccgc acgttcagac
    38761 tacatgggaa tgtacgccag ttacatggca caaaacggtt tttacagcga tctcgttata
    38821 aaagcatcgc gccagaaaaa tagtttccac gtactggaca gtcagaacaa cggcgttaac
    38881 gccaacggca ctgcgaatgg aatgagcatc tccctggaag ccgggcagag gttcaacctg
    38941 tcccctactg gttatgggtt ctatatagag ccgcaaaccc agcttacata cagccaccag
    39001 aatgagatgg ctatgaaggc gagtaatggc ctcaatatac atctgaatca ctacgaatcg
    39061 ctgctggggc gtgccagcat gatactgggg tatgacatca ccgcaggcaa cagccagctg
    39121 aatgtctatg tgaagactgg cgctatccgc gagttttcag gggataccga atatctgttg
    39181 aacgactccc gggagaagta cagtttcaaa ggtaatggct ggaataacgg cgtgggagtc
    39241 agtgcacagt ataacaaaca gcacacattc tatctcgaag cggattacac gcagggtaac
    39301 ctctttgatc agaagcaagt caacggagga tatcgcttca gcttttaagt gcgggataga
    39361 caagccctta aacgatgctg aaaattgtta gcgtgcgccg gaatgaaata cagatcactc
    39421 tgatgtatca gaaatcgttt ctgtctttca tacccggcgc ttaaactgcc ctgcatcaat
    39481 gccatgttgc ttcattttcc gccacagcgt agtgcggccg atcccaagta aagccgacat
    39541 ttcctgaatg cgaccgcctg tgacctgggc tgcgttaata attgcctctt tttcaacttc
    39601 cgcaaatgac agactggtgg aaaggcgggt ggcgctgaca tcatctgtcg cctgctcggt
    39661 aaacagatgt tcaggtaaat cactgacgcg aatgcgcccg ttatcactac tcagagcaag
    39721 attctcgatg acgctgtaaa gttcaaaatc gttgcctggc catgcacaag aaactaggcg
    39781 agcgagggca tcgtcatcaa ttttcagccg cgtagagaag cgtttttcaa gactgcgtaa
    39841 tttgttattc accagcgccg gaatgctgcc acgccgcata cgcagaggcg ggatggtaat
    39901 ttcaaatgca tgcagcgcgt aatacagctg gcgactaaaa cgattttgtt ccaccagcat
    39961 tgcgaggtcc gcggtcgttg ttgcaatcac tttgacatca attggtatta aacgccgcgc
    40021 atccagtcgc gtgataaccc cctgcttgat aacctgcagc aaagcagact gtaactccac
    40081 cgccagatat tcaatctttt caagaaacag cgtgccgccg tgtgccagtt ccagccgact
    40141 cagacggcca ttttcattgt ccgtgcgatc gccaccaata aattcttccg ccagcgcagc
    40201 atcaccatat aactcacaat tgacggcgat ataaggacct gcagcacgct cgctttcatt
    40261 atgaattgcc tgacttagca gtgccttgcc cacgccctct tctccacaaa gcaggacagg
    40321 aaagctactg cgcgccgcct ggcgaccaaa atgaatcaag cggcgggttt gcggatcgtc
    40381 ctgtggcata tgagcgaagg tatggctgac ttttcctaat tgactggtca ttaactgccg
    40441 catctgttcc acaggatgga gcaacaaaat aaagctggtt ccctgcgttt ctattatcgg
    40501 tttaagggtt atcaccgcat caataaactg gtgctggctt tcaaaggttg cttctacgtg
    40561 tttgagcgga tgtgccagtt ttattgcttg ttgcaatacg gcgggtaacg ttaagagttc
    40621 agtgattgcc cgtccctgac ttgccgtcgc gtcaaggcgc aagacccgcg ccgcctgggc
    40681 attaataaat tgcaaattac cctgctcgtc ccagctaatc acgccatcat ccatactttc
    40741 taacagggca ttaagttgat ttaaatgacg gttagtttca gcgagcaaac tgtccgtcag
    40801 cagtaaatta ccgacctcgc gggcgattgc caacgtcaac ggcaaatcag ctgcggtagt
    40861 ttgctcaacc ggacacgcca gcgctattgt tcccgtcaat cggcccttgc tgtcaaacaa
    40921 cggcgttgca caaaaggccc agttccagag tgcctgtttg aaatgttgat cggccatcgt
    40981 tttcacggcc tgaccagaga tagccgctaa cgatagcgca caagtaccaa taattccctc
    41041 ggcgcaatac gtgccgtcat tgaatcccag tgcacttagc tgctgcaagg tttgcggatc
    41101 gccattacgg ctgagaatgc aggcggtttc atcgaggata aacagcgcac actctcgcgg
    41161 tgccatatat tcccaggcat cttccagcgc agcctgcccg agcgtcagca tcgctttctt
    41221 acgccgataa atagaagcaa atgtcacgcc ctgggcctga tgtggtacgt tccatgtctc
    41281 ccgtttcatc agcttattgc atcgctccca ggaggttgca attaagggag atatgccccg
    41341 accatcgttg ttaaaagcgc cactcatatc ccgcatccct tatgacaaaa ggtgttccgg
    41401 ctcgcaattt taagtattaa tacacgtaaa agtttcaata tggaacattc attaaagcat
    41461 atgtttcact atgaaacata atttgggcta attttctttt cccttgcctg atgcacaatg
    41521 gattcatcgg cagtcagtgg tcgccgtgtc gttgaacatc atccatgccc taccgtaatt
    41581 gctggagcaa aataatgaaa aaattgatca atgatgtgca agacgtactg gacgaacaac
    41641 tggcaggact ggcgaaagcg catccatcgc tgacactgca tcaggatccg gtgtatgtca
    41701 cccgagctga tgcccctgtt gcaggaaaag tcgccctgct gtcgggtggc ggcagcggac
    41761 acgagccgat gcactgtggt tatatcggtc aggggatgct ttcgggggcc tgtccgggcg
    41821 aaattttcac ctcaccgacg cccgataaaa tctttgaatg ctccatgcaa attgatggcg
    41881 gcgaaggtgt actgttgatt atcaaaaatt acaccggcga tattcttaac tttgaaacag
    41941 cgaccgagtt actgcacgat agcggcgtaa aagtgaccac tgtggtcatt gatgacgacg
    42001 ttgcggtaaa agacagtctt tataccgccg ggcgacgcgg cgttgccaac accgtattaa
    42061 ttgaaaaact cgtaggcgca gcggcggagc gtggcgactc actggacgcc tgtgcggaac
    42121 tggggcgtaa gttgaataat caaggccact caataggtat cgctctcggt gcctgtaccg
    42181 ttcctgccgc gggcaaacct tcttttaccc ttgcggataa tgagatggag tttggcgtag
    42241 gcattcatgg tgagccaggt attgaccgcc gccctttctc ttcccttgat caaaccgtcg
    42301 atgaaatgtt cgacaccctg ctggaacatg gctcatacca tcgcactttg cgtttctggg
    42361 attatcaaca aggcagctgg caggaagaac cacaaaccaa acaaccgctc cagtctggcg
    42421 atcgggtgat tgcactggtt aacaatcttg gcgcaactcc gctttctgag ctgtacggcg
    42481 tctataaccg cctgaccaca cgttgccagc aagcgggatt gactatcgaa cgtaatttaa
    42541 ttggcgcgta ctgcacctca ctggatatga ccggtttctc aatcacctta ctgaaagttg
    42601 atgacgaaac gctggctctc tgggacgccc cggtccacac cccggccctt aactggggta
    42661 aataaggaga aagcaatgtc actgagcaga actcaaattg ttaactggct cactcgttgt
    42721 ggcgatattt tcaacaccga gagcgagtat cttaccgggc tggatcgcga gattggcgac
    42781 gctgaccacg ggctaaatat gaaccgaggc tttagcaaag tggtggaaaa actccctgct
    42841 atcgcagata aagatatcgg tttcattctc aaaaataccg gtatgacgct gctttccagc
    42901 gtcggtggtg ccagtggtcc gctgttcggt accttcttta tccgcgccgc acaggcgacc
    42961 caggcacggc aaagcctgac actggaagag ctttatcaga tgttccgcga tggcgcggac
    43021 ggcgtaatca gtcgcgggaa agccgaacct ggcgataaaa ccatgtgtga tgtgtgggtg
    43081 ccggtggtgg aatcgttacg tcagtccagc gagcaaaatc tctctgttcc ggtggcgctc
    43141 gaagctgcca gtagcatcgc cgaatccgct gcacaaagta cgattacgat gcaagcccgc
    43201 aaaggccgcg ccagttatct cggtgaacgc agtattggtc accaggatcc cggcgcgacc
    43261 tcggtgatgt ttatgatgca aatgttggcg ttagccgcaa aagagtaagg aattggtgat
    43321 ggtaaacctg gtcatagttt cacatagcag ccgactggga gaaggtgtcg gtgaattagc
    43381 ccgtcagatg ttaatgagtg atagttgtaa aatcgccatt gccgcgggaa ttgacgatcc
    43441 acaaaatccc attggtaccg atgccgtcaa agtgatggag gccatcgaat ctgttgctga
    43501 tgccgaccat gtgctggtca tgatggatat gggtagcgca ttattgagtg ctgaaactgc
    43561 gctggaattg ctggctcccg agatcgccgc aaaagtacgt ttgtgtgctg cgccgttggt
    43621 cgaaggtaca ctggcagcaa cggtcagcgc ggcctcgggg gcggatatcg acaaagttat
    43681 ctttgacgct atgcatgcgc tggaagccaa acgtgaacaa ctgggtttac cgtcctccga
    43741 cactgaaatc tctgacacat gtcctccgta cgatgaagaa gcccgttctc tggcggtggt
    43801 cataaaaaac cgtaacggcc tgcatgtacg tccggcctcc cggctggttt ataccttatc
    43861 gacatttaat gccgatatgt tgctggaaaa aaacggcaaa tgcgtcacac cagagagtat
    43921 taaccagatt gcgttactac aagttcgcta taacgatacg ctgcgcctga ttgcgaaagg
    43981 gccagaagct gaagaggcac tgatcgcttt ccgtcacctg gctgaagata actttggtga
    44041 aacggaggaa gtcgctccac ctactctgcg tcccgttctg cctgtttcgg gtaaagcctt
    44101 ttattatcaa ccagttttat gtacggtaca ggcaaaatca accctgaccg cggaagaaga
    44161 acaagatcga ttacgccagg ctattgactt cacgttatta gatctgatga cgttaacagc
    44221 gaaagcagaa gccagcgggc ttgacgatat tgccgcaatc ttttctggtc accatacact
    44281 gttaggtgat ccggaactgc tggcggcggc aagcgaactc cttcagcatg aacattgcac
    44341 ggcagaatat gcctggcagc aagttcttaa agaacttagc cagcaatacc agcaactgga
    44401 tgatgaatat ctacaagctc gctatattga tgtggacgat cttctgcatc gcaccctggt
    44461 ccacctgacc caaacgaaag aagaactccc gcagtttaac tcgccaacta ttctactggc
    44521 ggagaacatt tatccttcca cagtactgca actggatccg gcggttgtaa aaggtatctg
    44581 ccttagcgcc ggaagtccgg tatcccacag cgccctaatc gcccgtgaac tggggattgg
    44641 ctggatttgc cagcagggtg agaaactgta tgcgatacaa ccagaagaaa cgctaacgct
    44701 ggacgttaaa acgcaacgtt tcaaccgtca gggttaagcc accgttcgga tggcatcgtt
    44761 ctgatgtcat ccggcaattt acccagcact gatgtttttt aactacctgt tatatttttt
    44821 accaatttat gatgattttt cttcgctaat cgcgaaccaa atgtcgctgg ctggctaata
    44881 aacagacgtt atttgatcac tctatttcgc cgttatttat ccctttaatt cccttttcta
    44941 aaatgcctga ccgttcgcag aatgagattt cgatcatgca gctagtgcga tcctgaacta
    45001 aggttttctg atacttgaat accgttttat tcggtttcgc caaaggagaa tgattgatga
    45061 aatcccccgc accttctcgc ccgcaaaaaa tggcgttaat tccagcctgt atctttttgt
    45121 gtttcgctgc gctatcggtg caggcagaag aaacaccggt aacaccacag ccgcctgata
    45181 ttttattagg gccgctgttt aatgatgtgc aaaacgtcaa actttttccg gaccaaaaaa
    45241 cctttgccga tgccgtgcct aacagcgatc cgctgatgat ccttgctgat tatcggatgc
    45301 agcaaaacca gagcggattt gatctgcgcc atttcgttaa cgtcaatttc accctgccga
    45361 aagaaggcga gaaatatgtt ccgccagagg ggcagtcact gcgcgaacat attgacggac
    45421 tttggccggt attaacgcgt tctaccgaaa acaccgaaaa atgggattct ctgttaccgc
    45481 tgccggaacc ttatgtcgtg ccgggcggac gttttcgcga ggtatattac tgggacaatt
    45541 acttcaccat gttaggactt gccgaaagtg gtcactggga taaaatcgcg gatatggtgg
    45601 ccaattttgc tcatgaaata aacacttacg gtcatattcc caacggcaac cgcagttact
    45661 atttaagccg ctcgcaaccg cccttctttg ccctgatggt agagttactg gcgcagcatg
    45721 aaggcgatgc cgcgttgaag caatacctgc cgcaaatgca aaaagaatat gcttactgga
    45781 tggacggtgt tgaaaacctg caagccggac aacaggaaaa acgcgttgta aaacttcagg
    45841 atagtaccct tctcaaccgc tactgggacg atcgcgatac gccacgacca gagtcatggg
    45901 tggaagatat tgccaccgcc aaaagcaatc cgaatcgacc tgccactgaa atttaccgcg
    45961 acctgcgctc tgccgctgcg tctggctggg atttcagctc gcgctggatg gacaacccgc
    46021 agcagttaaa taccttacgc accaccagca tcgtaccggt cgatctgaac agcctgatgt
    46081 ttaaaatgga aaaaatcctc gcccgcgcca gcaaagctgc aggagataac gcgatggcaa
    46141 accagtacga aacgctggcg aatgcccgtc aaaaagggat cgaaaaatac ctgtggaacg
    46201 atcaacaagg ctggtatgcc gattacgacc tgaaaagtca taaagtgcgc aatcagttaa
    46261 ccgcggccgc cctgttcccg ctgtacgtca atgcggcagc gaaagatcgc gccaacaaaa
    46321 tggcgacggc gacgaaaaca catctgctgc aacccggcgg cctgaacacc acgtcggtga
    46381 aaagtgggca acaatgggat gcgccaaatg gctgggcacc gttacagtgg gtcgcgacag
    46441 aaggattaca aaactacggg caaaaagagg tggcgatgga cattagctgg cacttcctga
    46501 ccaatgttca gcacacctat gaccgggaga aaaagctggt ggaaaaatat gatgtcagca
    46561 ccaccggaac ggggggcggc ggtggcgaat atccattaca ggatggcttt ggctggacca
    46621 atggcgtgac gctgaaaatg ctggatttga tctgcccgaa agagcaaccg tgtgacaatg
    46681 ttccggcgac gcatccgacc gttaagtcag caacgacgca accctcaacc aaagaggcac
    46741 aacccacacc ttaaccagcg cttactccga caacgtcatt cggcttctcc ggatggcgtt
    46801 gtcggcagat aacaccatat ctatggtttt ttctacacag acggtttttc aagtcctggc
    46861 gggaagtatt gcgcaaatgt ttgccatacg tggttgccga agatagcgtc atttctacgg
    46921 gtaagtgttt cactttcatc acccggcgat ttacgccgac gttggctata taaagcatca
    46981 attgctaccg aatattgtta cggtgttcac tcatcaacgt ttttttgctt tctggagtta
    47041 aattaataat cacataatcc agacactcca gagaatttag caaccaaaaa aagaattcag
    47101 accattcctc ttcgcgtaac tgaagcattg ccgaacgggt attttggctt aatctgccag
    47161 aaggattgat caattctaaa tgttcaataa gcgcagccaa aaccgcctca ctatccgccc
    47221 catttttaac gcctgactgg ctactgtcta aaatatttat tttctgaatc acaccaaaaa
    47281 caacgaaaca ataattaaaa aaggattctg ttgttgttac cgggaaccga cttatttcgc
    47341 tcagaaagtc gttcaaccag ctccgcagtg ataagattcg ctgatgatac agtgtatcct
    47401 cactgcgtgc tggaggtgcc agtataatat gttgatcaat acagtattta ttgtgttcag
    47461 tcgtttcctg atgcgtttgg ttctctgcgg attgtcgctg gcgaaataaa tggcttgcgg
    47521 taaatgccgt tacagagcag cgcaacgcgt caattccttc ggagtgcccg acttttggcg
    47581 agagttccgg ttttatgcgt ccaggtagcg gaaatttcac ccgaatgaga aatgcattaa
    47641 taaaat
//
LOCUS       DAJTRN010000021        47423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRN010000021 DAJTRN010000000
VERSION     DAJTRN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            90..572
                     /gene="sseA"
                     /locus_tag="OO328_001224"
                     /pseudo
     CDS             90..572
                     /gene="sseA"
                     /locus_tag="OO328_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO328_001225"
     CDS             complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO328_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5929857.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO328_001226"
     CDS             complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO328_001226"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5929858.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO328_001227"
     CDS             complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO328_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5929859.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO328_001228"
     CDS             complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO328_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5929860.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO328_001229"
     CDS             complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO328_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5929861.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO328_001230"
     CDS             complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO328_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5929862.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO328_001231"
     CDS             complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO328_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5929863.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO328_001232"
     CDS             complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO328_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5929864.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO328_001233"
     CDS             complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO328_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5929865.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO328_001234"
     CDS             complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO328_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5929866.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO328_001235"
     CDS             complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO328_001235"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5929867.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10375..11178
                     /gene="suhB"
                     /locus_tag="OO328_001236"
     CDS             10375..11178
                     /gene="suhB"
                     /locus_tag="OO328_001236"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5929868.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11323..12177
                     /locus_tag="OO328_001237"
     CDS             11323..12177
                     /locus_tag="OO328_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5929869.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12368..13648
                     /gene="csiE"
                     /locus_tag="OO328_001238"
     CDS             12368..13648
                     /gene="csiE"
                     /locus_tag="OO328_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5929870.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13640..14779)
                     /locus_tag="OO328_001239"
     CDS             complement(13640..14779)
                     /locus_tag="OO328_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5929871.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO328_001240"
     CDS             complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO328_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5929872.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15965..17326
                     /locus_tag="OO328_001241"
     CDS             15965..17326
                     /locus_tag="OO328_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5929873.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17323..17841
                     /locus_tag="OO328_001242"
     CDS             17323..17841
                     /locus_tag="OO328_001242"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5929874.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17841..18161
                     /locus_tag="OO328_001243"
     CDS             17841..18161
                     /locus_tag="OO328_001243"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5929875.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18158..18970
                     /gene="hcaB"
                     /locus_tag="OO328_001244"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18158..18970
                     /gene="hcaB"
                     /locus_tag="OO328_001244"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5929876.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18980..20197
                     /locus_tag="OO328_001245"
     CDS             18980..20197
                     /locus_tag="OO328_001245"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5929877.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20294..20716
                     /locus_tag="OO328_001246"
     CDS             20294..20716
                     /locus_tag="OO328_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5929878.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20764..21636)
                     /locus_tag="OO328_001247"
     CDS             complement(20764..21636)
                     /locus_tag="OO328_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5929879.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLTPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21648..22742)
                     /locus_tag="OO328_001248"
     CDS             complement(21648..22742)
                     /locus_tag="OO328_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5929880.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22775..23773)
                     /locus_tag="OO328_001249"
     CDS             complement(22775..23773)
                     /locus_tag="OO328_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5929881.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23798..25309)
                     /locus_tag="OO328_001250"
     CDS             complement(23798..25309)
                     /locus_tag="OO328_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5929882.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25332..26314)
                     /locus_tag="OO328_001251"
                     /pseudo
     CDS             complement(25332..26314)
                     /locus_tag="OO328_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26411..29512)
                     /locus_tag="OO328_001252"
     CDS             complement(26411..29512)
                     /locus_tag="OO328_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5929883.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29519..29692)
                     /locus_tag="OO328_001253"
     CDS             complement(29519..29692)
                     /locus_tag="OO328_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929884.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29810..31003
                     /locus_tag="OO328_001254"
     CDS             29810..31003
                     /locus_tag="OO328_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5929885.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31201..32454)
                     /gene="glyA"
                     /locus_tag="OO328_001255"
     CDS             complement(31201..32454)
                     /gene="glyA"
                     /locus_tag="OO328_001255"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5929886.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32781..33971
                     /gene="hmpA"
                     /locus_tag="OO328_001256"
     CDS             32781..33971
                     /gene="hmpA"
                     /locus_tag="OO328_001256"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5929887.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34016..34354)
                     /gene="glnB"
                     /locus_tag="OO328_001257"
     CDS             complement(34016..34354)
                     /gene="glnB"
                     /locus_tag="OO328_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5929888.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34415..35749)
                     /gene="glrR"
                     /locus_tag="OO328_001258"
     CDS             complement(34415..35749)
                     /gene="glrR"
                     /locus_tag="OO328_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5929889.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35739..36452)
                     /gene="qseG"
                     /locus_tag="OO328_001259"
     CDS             complement(35739..36452)
                     /gene="qseG"
                     /locus_tag="OO328_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5929890.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36617..38044)
                     /gene="qseE"
                     /locus_tag="OO328_001260"
     CDS             complement(36617..38044)
                     /gene="qseE"
                     /locus_tag="OO328_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5929891.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38602..42489)
                     /gene="purL"
                     /locus_tag="OO328_001261"
                     /gene_synonym="purI"
     CDS             complement(38602..42489)
                     /gene="purL"
                     /locus_tag="OO328_001261"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5929892.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42748..44304
                     /gene="mltF"
                     /locus_tag="OO328_001262"
     CDS             42748..44304
                     /gene="mltF"
                     /locus_tag="OO328_001262"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5929893.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44301..44837)
                     /gene="tadA"
                     /locus_tag="OO328_001263"
     CDS             complement(44301..44837)
                     /gene="tadA"
                     /locus_tag="OO328_001263"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5929894.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44862..45497)
                     /gene="yfhb"
                     /locus_tag="OO328_001264"
     CDS             complement(44862..45497)
                     /gene="yfhb"
                     /locus_tag="OO328_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5929895.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45706..46554
                     /locus_tag="OO328_001265"
     CDS             45706..46554
                     /locus_tag="OO328_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5929896.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46893..47423)
                     /locus_tag="OO328_001266"
                     /pseudo
     CDS             complement(46893..47423)
                     /locus_tag="OO328_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11305 a        12987 c        11934 g        11197 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg gacttgcagg ctggcagcgc gatgacctgc
      121 tattagaaga aggtgcagta gagctgccgg aaggagagtt taacgccgcg tttaatcctg
      181 aagcagtggt gaaagtaacc gatgtgttat tggcaagcca tgaaaatacg gcgcaaatta
      241 ttgatgcccg cccggctgca cgttttaacg cagaagttga tgaacctcgc ccaggtttac
      301 gtcgcggaca tattcccggt gcattgaacg ttccgtggac ggaactggtg cgcgaaggcg
      361 aactaaaaac gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc agctatgaca
      421 aaccgattat cgtcagctgc ggctctggtg taacggcagc cgtggttttg ttagcactcg
      481 cgacgctgga tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa tggggcgcgc
      541 gggcagattt accggttgag ccagtgaaat aagtatatta caggcaataa aaaaccgccg
      601 aatttggcgt ttttttattg ctggtctggt tcgcggcctt tccagcaggt tgacttgtgt
      661 tacatgagca acgcaggtgc ttcacagcaa aacaatactc accagtaact ctctttttgt
      721 caagcaaaag agagtaatta ttgtttattt agcgtattat cgacaccggc cctttccgcc
      781 gtgttcggta ataaaataac ctggcttatt agtccgaatt cagacaaata taaacaaatc
      841 ctgctcaaaa ttaaaaattc taaccggtaa aagatattac ttaaacatgt aaatttactt
      901 tcctttaaaa aacaaaaaac cgccaaaatc aggcggttat ttgttgctgg tccggttcgc
      961 ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac
     1021 tcgccagtaa ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt
     1081 atccactgcg gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc
     1141 cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg
     1201 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta
     1261 cttattgttt ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac
     1321 aatttactgg tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg
     1381 gcggcatatt ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac
     1441 gttcatagaa tggcgcaata tgttcggtaa taaagtggct aattcctttt tccccttttt
     1501 tcacctgaca aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg
     1561 tcgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct
     1621 gtgcctcttc gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt
     1681 taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata
     1741 tcagcgattc accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa
     1801 tcaacaaact gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga
     1861 agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct
     1921 gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg
     1981 tgccgtcttc tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc
     2041 cctgcgccgc cgtaccaggc acccagacgg tggattccag tagagtacgg aaaaaggccg
     2101 ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt
     2161 cggacataag aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct
     2221 tatcaggcct acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag
     2281 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca
     2341 caccaagtcc cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
     2401 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
     2461 ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
     2521 tagacggcag ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct
     2581 cttgtgaggc actcgccagt aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
     2641 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
     2701 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg
     2761 catcagtgtt catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct
     2821 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
     2881 tcagtccgcg cataatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
     2941 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
     3001 tgataccttt acctaccagg cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt
     3061 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg cccgacagtg tgcagcccca
     3121 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
     3181 cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg
     3241 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
     3301 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc gggcctttat
     3361 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat
     3421 gcttgatgcc cagaccgtca atcttgcggg ccgcacgctg gatcagcccc agatcgtctg
     3481 ccccgttcaa atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc
     3541 agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta
     3601 tccttattcg tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct
     3661 tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg
     3721 tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc
     3781 ggatcaagat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccac
     3841 ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga
     3901 tttcgaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct
     3961 ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt
     4021 caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt
     4081 gttcaatctc aataccgtta cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt
     4141 ccagaacagc gccatcaggg cagaggtcct gatgaggcaa aataacaatc tttggcatat
     4201 taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga
     4261 gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca
     4321 tcatcgccct gcgccacttc actcaggtga gcggcagcat tgtcaatgac ctgacgctct
     4381 gcggcgctta acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc
     4441 gccgcttcta ctttttgttc tgccagcata cgggctttta cgtcctgctc ggcatagctc
     4501 attgagtctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc
     4561 tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg
     4621 gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacca
     4681 cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt
     4741 acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc
     4801 accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg
     4861 cccagcgaca gtgggatcac atcaagcagc agcatttcgc tgtctggctt gttacccacc
     4921 agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc
     4981 agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa
     5041 ccgcccacca tcaccacttc cagcacttcg tcagcttcta cacccgcgtc tttcagcgcg
     5101 cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca
     5161 cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc
     5221 gcgattttgg ctgcaatggt ggcatccagc agttcacgct gaacgcggtt atcgctacga
     5281 tcaggaatgt ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg
     5341 ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga
     5401 atggaaatat caaacgtccc gccaccgagg tcataaacag cgatcacgcc ttcctgaccg
     5461 gaatccagcc cgtaggcgat agccgcagca gtcggttcgt taagtaagcg cagaacatgc
     5521 aaccccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc
     5581 ggaacggtga taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc
     5641 agtgctttga ggatgtccgc agaaacgcgc accgggttca gtagccccgc cgccgtttca
     5701 atcatcggca ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc
     5761 tggatatcag ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggtg
     5821 tcaagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga
     5881 acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg
     5941 cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc
     6001 tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc
     6061 cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc
     6121 gagaaaacgc agcttacgca ccgtatccgc cgccgcgtcc cacgcctcgt tgtctaactg
     6181 ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc
     6241 cagccgcgct tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg
     6301 ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg
     6361 taaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt
     6421 aatggttgca gattgctgta cggcggcgag ttgttccgcc aggcttccgc tggcgaattt
     6481 atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagtgcct gggtatcgag
     6541 ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg
     6601 atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg
     6661 ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg
     6721 ttcgggttgg tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta
     6781 ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac
     6841 acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat
     6901 ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta
     6961 tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta
     7021 ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag
     7081 aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt
     7141 gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga
     7201 agcgatagcg gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc
     7261 gttgacttta atctgcaact tcattacgtc gccacaggcc ggtgcaccca ccatgccgct
     7321 gccgacgttc tcgtcgttgt tatcaaagga acccacgtta cgcggattct cgtaatggtc
     7381 gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg
     7441 agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag
     7501 gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc
     7561 agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc
     7621 cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga
     7681 aactgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac
     7741 gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc
     7801 tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat
     7861 ctcttctttt gcgatgcgat aggcttcgcc cattccgacg atctggtgaa caggcagagt
     7921 gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacacg
     7981 cggtttacga cgcacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga
     8041 gaaagacatc aggtcaactt tcaactggct caggtcgatt ggcagtttgc ccacgctctg
     8101 ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat
     8161 atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc
     8221 acgcatcgcc gcttcgagtt ctttcaggtc gataataccg ttacgctgcg gtgccaggta
     8281 ggtgacttcg aaaccttcgc gctccagctg acggcaagta tccagtaccg ctttgtgttc
     8341 ggttttgctg gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat
     8401 cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc
     8461 gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa
     8521 acggtgagaa cgagaggccg ggttaccaaa ggttccgtcc atcgtcatga actgcatcat
     8581 tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa
     8641 tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga
     8701 tgtacgaccg tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta
     8761 agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg
     8821 ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt
     8881 gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt
     8941 atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac
     9001 ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc
     9061 gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc
     9121 cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc
     9181 ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag
     9241 tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg
     9301 gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt
     9361 gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct
     9421 gttgtcggat gcggtgtgaa caccttatcc gacctacgat tctgtacatt gtaggcctga
     9481 taagacgcgt taagcgtcgc atcaggcaac cactgttatt cggccttgtt acctttattc
     9541 tgctgctcaa tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc
     9601 gcacgggtaa acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata
     9661 aaaccggttg ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc
     9721 agcggatacg gcgtctcttc atgctcgacc tgctcgccat tttcctgagt tgccagccag
     9781 gccatacgca cttcataggc aataacctga accgccattg ccaggttcag cgaactgtat
     9841 tccgggtttg ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag
     9901 ccgacgcgct cacgaccaaa caccagcgcc accggggtat ttgccgcttc agcgacgctt
     9961 ttcagaccgc attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg
    10021 gtgcccacca ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca
    10081 ttaccaatca catcgctggc ccctgcagcc agggcaatcg cctgggagtc gggtttcacc
    10141 agtggattaa ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa
    10201 cccatattgc cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc
    10261 tttcttcatc taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc
    10321 gctgctatac tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat
    10381 ccgatgctga acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac
    10441 tatgaaaccc cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac
    10501 gtagataaag ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac
    10561 accatcatca ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt
    10621 atcgatccac tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct
    10681 atcgccgttc gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac
    10741 gaactgttca ccgccactcg cggtcaaggc gcacagctga acggctaccg tctgcgcggt
    10801 agcaccgctc gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa
    10861 cagtacgcca ctacctacat caacatcgtc ggcaagctgt tcaacgaatg tgcagacttc
    10921 cgtcgtaccg gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt
    10981 ttctttgaaa tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa
    11041 gcgggcggca tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc
    11101 gttgctggta acccgcgtgt tgtgaaagcc atactggcga acatgcgtga cgagttaagc
    11161 gacgctctga agcattaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca
    11221 ggcgctattc cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct
    11281 ttccccgatt aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac
    11341 aaacgcgttc ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg
    11401 ccgcgcgttg ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc
    11461 tggtcggcgg aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt
    11521 attccttctt cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc
    11581 aatgccggaa atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc
    11641 aacgttttta tgtttgatta tcgcgggttt ggtaaatcaa aaggcactcc gtcccaggcc
    11701 ggattgctgg acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac
    11761 ccacaacagc tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt
    11821 ggtcagggtg atcgtgaagg catacgtgcg gtgatcctcg actccacatt tgcctcttat
    11881 gcaaccatcg ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc
    11941 ggcgaaaatt acatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagcc
    12001 gatcacgtta tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa
    12061 cggttgatcc taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcaat
    12121 gtttatcgcg aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc
    12181 atgatcgcta gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc
    12241 agaacagttt aaaatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat
    12301 tcccttcgcc atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga
    12361 tctacagatg atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg
    12421 ccagatcttg ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga
    12481 gcttaatggt gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat
    12541 cctgcgctat catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg
    12601 tacagtgctt aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg
    12661 tccgtcattt attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg
    12721 aattgcgcgt aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg
    12781 acggctgcaa aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata
    12841 ttgtttgctg cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg
    12901 ggcggaatcc tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg
    12961 ggcgctccag catgtaccgc ctgatgagcc actgtttatg gcattacttt tttccatgtt
    13021 gcgggttccc gatccactgc gggatgcgca tcagcgggac agacaattgc gtcagtctat
    13081 caaacgtctg gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg
    13141 gttatgtgat cagctttata tccacctcgc ccaggcgtta aatcgcagtt tgtttgccat
    13201 cggtattgat aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac
    13261 cacccgcgcg gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga
    13321 aagtggtctg gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga
    13381 aaaacagatt attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcagca
    13441 gttacgcgaa ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt
    13501 gcagacaggc gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccgttacc
    13561 gctcttttca ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca
    13621 gatccgcaaa atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg
    13681 gaagcgccac cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac
    13741 cggcgaaaac ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct
    13801 gtaaacggat gacttcgcta acctggcgag cagcaatata acgcatggcg gccaggtggc
    13861 agaccgtgaa ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag
    13921 ttgctcccat aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc
    13981 gagcactaca acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca
    14041 cgcccagcga ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa
    14101 tggcgctaaa accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa
    14161 agcgccagtt ctggcgaacc agcgccaacc acgcagacca accggtgctc tcctgctggc
    14221 ggcttgcccc ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca
    14281 cgcccaacgt caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg
    14341 tcagcgccga gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa
    14401 gcgggaactg cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga
    14461 aaaagaggtt aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga
    14521 aggcgacagc aaagagaagt ggcagcagtg ccagcacgcg caaggcggaa atcaggcggg
    14581 aaggatcgct gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg
    14641 cccccaataa caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc
    14701 agaaaggtaa aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc
    14761 gcgtggattg caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga
    14821 atgttggtgc aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc
    14881 agttcacaag cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt
    14941 atgccgttac gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccagct
    15001 tcatctcccc tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg
    15061 gacgaaaaac aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc
    15121 ccagccccat gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat
    15181 ttggctggct gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat
    15241 acgccggatc ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt
    15301 catgtgctaa agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca
    15361 gataatcaat ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac
    15421 ggcgaatttt ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg
    15481 gctgtctgag acgaaacatc ggtaatactt ttggcaataa attcacttcc gccgatggca
    15541 caaagccaat ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat
    15601 tttccgcttg ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca
    15661 acgcgacttt gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc
    15721 ggatctggct gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt
    15781 tcagtgcctg cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct
    15841 tgttatcgaa aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac
    15901 cagcaacatc cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa
    15961 aaatatgacc acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg
    16021 ggtcactccg cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt
    16081 cggtcgttgc tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt
    16141 taacacctac atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa
    16201 ggcgtttctc aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgtggcaa
    16261 cagtcgcgcc tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat
    16321 cgatgtaccg ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct
    16381 aaacgaagtt ccttgtgtgg agagttataa aggactgatt tttggcaact gggataccag
    16441 cgcaccgggc ctgcatgatt acctgggtga cattgcctgg tatctggatg gcatgctgga
    16501 tcgtcgcgaa ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa
    16561 ctggaaattc ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc
    16621 ttctgccgtt caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg
    16681 acaaaccgcc cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg
    16741 tcacggtagc ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc
    16801 agtttcaagc tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg
    16861 cgccctgcgc ctggcgggtc ataacaatat attccccacg ctttcatggc taaacggcac
    16921 tgccacactc cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg
    16981 tattactgac aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg
    17041 tgcttttggt cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca
    17101 gaaattgctt aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg
    17161 tcaggaaaag cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac
    17221 tgccgctcgt ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga
    17281 agtgctcgat aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca
    17341 ctggagttac atcaccgcat tagccagttt ctctttcacg aagccagtct gctggatgac
    17401 tggaaatttc gtgactggtt ggcacagctc gacgaagaga ttcgttacac catgcgcacc
    17461 acggttaacg cgcaaacacg cgaccgtcgc aaaggcgtcc agccgccgac gacctggatt
    17521 ttcaatgaca ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggcc
    17581 tgggcagaag agccgccgtc acgcactcgt cacttaatca gcaactgcca ggtaagcgaa
    17641 accgacatcc caaacgtatt tgccgtgagg gtgaattatc tactttatcg ggcacaaaaa
    17701 gagcgcgatg aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat
    17761 gacaactggc gcttgctgga acgggatatc gtcctggatc aagcggtgat cacttcccat
    17821 aacctgagtg tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg
    17881 agggtgaggc tctccggata gatacctcgc ccgtcatcgc cttgttcaac gtgggcggcg
    17941 agttttatgc cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc
    18001 tggaagatga cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg
    18061 ggaaagcgtt atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag
    18121 gtggtgacat tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc
    18181 catttttatt accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga
    18241 agaaggcgcg caagttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca
    18301 gcgatttggc gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca
    18361 acgcgcggtc gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc
    18421 aggcatctgg gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg
    18481 cttccacgag ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa aagcctgcgc
    18541 tccggcgtta atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta
    18601 tcctggcggc ggtggcccgc tgtacaccac cagtaaacat gccgcaaccg gacttattcg
    18661 ccaactggct tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat
    18721 ggccagcgac ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga taatgcagtc
    18781 tctgacgccg gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga
    18841 ttttacgggg ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt
    18901 gatgatcaac gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct
    18961 ggatctttaa ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg gcgggcaagc
    19021 ggcggcaatg gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt
    19081 ttccgatgag cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga
    19141 agattccccg cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca
    19201 tctgcattcc ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac
    19261 caacggcgaa agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc
    19321 gctgccgttg cttgatgcac tgggagaacg ctgctttacc ctacaccatg ccggtgatgc
    19381 cgccagactg cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac
    19441 tattggtctg gaactggctg ccagcgccac gcagcgcagc gcagcgcagc gcagatgtaa
    19501 ggtgacagtg attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca
    19561 acgctatctt ttacagcgcc atcagcaggc tggtgtgcgc attctgctca ataatgccat
    19621 tgaacatgtg gtcgatggtg aaaaagtaga actgacgctg caaagtgggg aaacgcttca
    19681 ggctgatgtg gtgatttacg gtattggtat cagcgccaac gagcaactgg ctcgcgaggc
    19741 caaccttgat actgccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc
    19801 gatctttgcc ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg
    19861 cgaaagctgg gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa tgttagggct
    19921 accgctaccg ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca
    19981 gtttattggc gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa
    20041 ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg
    20101 ccgtgagatt cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact
    20161 gctgatagat gagaacatcg cgcttaaatc actgtaatca ggataattag cgaatatctc
    20221 aatgcctggg gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc
    20281 aaagggattg gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt
    20341 gttattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat
    20401 gatgggcttt gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct
    20461 ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt
    20521 tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg
    20581 tcaccattac tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa
    20641 gaatgtcagt atcgctggcg cgttcttgct attggcaatt accggaccgg gggcaatttc
    20701 tctcgatcgg cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat
    20761 aaattacagc cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag
    20821 cgcaatgaga tcaccccctt ccgggcgatg atgctcatcc ggcgcatggc tcatcggttc
    20881 cagacagaag aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa
    20941 ataacacggt gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc
    21001 gatgcgggcc tgaccattcc atccggcaaa accattgttc acccactgac gcggcaacgg
    21061 cgtgagctgg ttaaaatcca gttcctgcgg tagctgctcg caaaactcac ccgccagcca
    21121 ctgctcccgt tccagccagt aaccgctcgc ctgcgcctga atccgcgttt gtggtgacaa
    21181 cggaaaataa ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac
    21241 agagagcgtc accgtcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg
    21301 atagacaccg ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca
    21361 ttgccactcg cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg
    21421 ttgcagttga tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa
    21481 tggcaccagc ggaaaacatg aggcatcggt tgccacaccg ctttttttac caggacgtaa
    21541 gagcggcgtc gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgtccag
    21601 ctttagcgac ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag
    21661 ttaatcacaa ctttcccgca tttgccgctc gccatcagca cataggcatc acctgcctgt
    21721 tctagcgaga agcgatgggt aatggcgtta cgcggccaca gcttccagtc agtcaaatcg
    21781 tgggcgcatt tttccatatg gaacagactg gtcacccagg agccgataat ccgccgttga
    21841 tggtgcatca gatcagcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc
    21901 acccgccccc agtcagcagt ggattgcagt gccagcaagc gacctgcggc attaccggaa
    21961 caatcgagcg caacatccgc gccaccgtgg gtaagttcag caattatctg cggcagacct
    22021 tcggtggttg ctaaatagcc gtgatccatc ccccctaact gttttgccat cgccagacgt
    22081 tccggcagca tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc cagcatcatc
    22141 gccatcatgc cgaccggccc cagaccgacc accagcacgt tatcgctgcc ggaaacttcg
    22201 ccgcgcaaaa tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg
    22261 tagctcagcg catccggcag gaggatcaga tctttttctt ccgccagcag atattcggca
    22321 tgaccgccgt cacgctgcca gccgtaagcc gcttttcctt tgccagtaca agagatagga
    22381 aagccgcggc ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg
    22441 tcgccctctt taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca
    22501 tgaccgttga taaatccctg gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt
    22561 tgatgataga tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt
    22621 acctggttaa tccccggcgt cggcaccgca acttcccgca gatcgacggt tgaatttcct
    22681 agtaaataag cagccagcat cgttttcata aatccctcat taacaatacg attaattttc
    22741 atccctgcag cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgcttcgct
    22801 gggtcagcaa gatattcgcc agcaccgcca ccacgatgat gacgccgcgt accacctgct
    22861 ggaaaaagga gttaataccg agcagcacca gaccgttacc gattagcgta atcaccagca
    22921 caccaagcaa tgtaccgaac agggaaccgc gaccgccgga aagtgccgta ccgccgacca
    22981 cgaccgcggc gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga
    23041 ggcgtgccgc caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa
    23101 gaatgcgcac tcgacgaacg ttaataccgc acaactgcgc cgccgtggca ttaccgccaa
    23161 cggcaaaaac cgagcgcccg aaggcggttt ttcggctaat gaacacaaac agcgcaaaca
    23221 acaccatcat gatcagcgcg gatactggca caccgagaaa ttgtccgccc agccagtcca
    23281 gcacctcgtt ttcgtcaatc ggcactggca gtgcgttggt cataaacagc cccattccgc
    23341 gcagagcgct ccacagcccc agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca
    23401 gcaccccggc gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg
    23461 ccaacggaac ttcaaattgc agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg
    23521 gcccaacgct gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa
    23581 tcccaatggt ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag
    23641 gcgcgttcag ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat
    23701 taatatggcg actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg
    23761 acgaagcata catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg
    23821 acatcagctc atccacattg accggcgagt gaaactcctg cgagaacgtg ccgtgctgta
    23881 ataacaggat gcggtcacac accagcggca attcctccac ttcactggag ataaacacca
    23941 cgctttttcc ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga
    24001 tatcgacgcc gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac
    24061 gaccgatcac cactttttgc tgattgccac cagaaagcgt gccgatgggt gtttcgctac
    24121 tggcagcctt gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc
    24181 attgcagcac accgttggtg ctgatttttt gccgattggt cagcactgta ttttcgtcaa
    24241 cgcctagcca gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc
    24301 gtttcagcat gtcgccgtaa tcggggcgca tgattttctc gccgttgata acaatttcgc
    24361 cctgttcata cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca
    24421 gcaggccagc aatgccaagc acttcgccac gacgtagcgt aaagctgata tcctccagct
    24481 tgggcttatg gcgtaacgca cggacttcca gtacggcctg atccacaatt tcctgagggg
    24541 caaccggcgc aatatcaacg tgatcacgcc ctagcattag cgacacaata tgatgcgtgg
    24601 aggtgttttc gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcatagg
    24661 aggcaatgcg gcggatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg
    24721 ccgacatctt tttcaccgca ctgatcacca gttcaacttc tgcactggca agcgagctgg
    24781 tgggttcatc aagaatgacc acgcgcggct cgcccttcat cacccgtgca atttccacca
    24841 gttgcttttg cgccgggctt agcgttgaaa caagttgttc aggactaacg tcaacgccca
    24901 gcgcctgtaa gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc
    24961 ggcgaggcca ttgaccaagg cagaggtttt ctgccaccgt cagcccttcc accagactta
    25021 attcctgata aaccgcacga acccccagtt cagcggcacg gcgagtcaac gtagcttcgt
    25081 caccttccag tcgcgtctcg ccaatccaga tatcaccgct atccgggcgt tcgctgccgg
    25141 taagcattcg aatgagagtc gatttgcccg cgccgttttt acctaacagc gcacgaactt
    25201 cgcctttatt gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg taacgcttat
    25261 ttcctgccac cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg
    25321 cgaatcagcg attagggcag accatcaacg tgcgttgcca gccactgttt accatcttcc
    25381 gttttggtat agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg
    25441 ataaccttca atgtttgcgc gaaaacagca ttgcccattt tcttaccgga aatatccact
    25501 accgctttca gcacctgatt gttttccagc tcctgagcaa tttcggttgt catatccgaa
    25561 ccgaaaacag caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt
    25621 gtcgcaccgc ccgactcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt
    25681 tcaccaacgg aaatcgcttt atctaaaaca gtcccttcct ggttagcgac aatttgcgcg
    25741 ccgggaacgc gggattttaa tacttcttca aatcctttac gtcgctgcac acaaacttca
    25801 aaggcttcgc aattgatgac ggcaattttc ggctggtcaa ttttattggc aataaaataa
    25861 tcggcggcag cgttacccag tttttaccaa attccagtgg atcgccgacc agatacgccg
    25921 agacatattt atcgacaccc ttttgattaa tacaggtgtt gtagcaaatc accggaatac
    25981 ccgcttcact ggcgcgacga acggtacggc tactgccatt ttcagacact gccgataaaa
    26041 taatggcatc gacattacgc gccacgaggg tatcaacaaa ggtactttct ttcgaaatat
    26101 ccccctgggc gttagtttca attaactgta cctgtactga agaatctttt gccgcatcct
    26161 gaacgccctg acgcactcca gcgtaatatc cctgggtatc aaggtatatt gcaccaatgg
    26221 tcatttcttt atccgcagcc ctggcaaata gtgcgctacc taatagcgta gccattaata
    26281 atagattacg ggttgttctc atttttttag gcatagtgcc tcctgtaggg tttttattaa
    26341 caacggctta ttctaattat tttgtgatga gcggcagcgc gtgccgctca aatattacag
    26401 ttaatagggt ttagtgaacg tagttaaaaa taaaaggcat cacagtggtg aataatgccg
    26461 ctttcggcca ggccggatta atcgccgtca attcagcgcg agcagactca tattgcgcaa
    26521 cctccccgag tcccgcggac gccagcatcg ccaccatcag gcatttttct ttatgctgct
    26581 gttgtaaatc gccatacagc gataacaggt caggttgtga aacagcaaag aaatccgcct
    26641 caatactggt tttcgccatc tcttgcgccc actgtttcat ttcactaaac agttgctgtg
    26701 cggtttgctg ttcacccagc agtcgcagcg ccattccttg ccagaagaga taatcaaccg
    26761 gctgatcgtt gtaataactg tggatgttaa tggtgcgatc gccggtcgcc gccagacgta
    26821 aacaacgcat cgcttcagtt tcatcgccct gcgcgttggc gcatatcgcc tgccagaacc
    26881 agatgtcgtt atcagtttgc cccggtaaac ggccttcgct taaattctcc ggataataca
    26941 gcgcggcatg aagcagttcg ctggcctgct gcggctctct ggcatcaaga tgctgccagg
    27001 cgcgtaataa ctggttgagg ataaactgac tggtgacctt cccttccccg ccttcccacg
    27061 ggtggaattt acgcgtggcg agaatgtccg ccgctttgtc tgcctgaccc gtgagatgcc
    27121 acaaattgag cagttctgcg gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt
    27181 ccagacgcgc cagtcgtttc tccggtgtgg ctccacttaa cttatccagc aaatcccgtt
    27241 cgaaaagcag acgtgcatcc tgcggcgcaa gctgataagc attatcaaga taacgcgcgg
    27301 ccagctcata atcgtgtttc ttattccacg catggatcgc taacccgcgc cagccgtcgg
    27361 caaactccgg cgacatctct acgcaacgtt gccataaggc aatggctttg ttgtagctac
    27421 gtttgttgta gtagaagcag gccagtaaat ggcgagcaaa ccaacactct tcaatactct
    27481 ccagcgccgc cacttcttcc agcgtattcg ggaaacggac aaactgcggg aagacatcaa
    27541 tggcttttgc gaccagttcg ccacgttcgg ctttcggcag caagctggct tgcaggtaaa
    27601 gcggcagcgt gcgctggcag tccagtgcgt tcagcatctc tgccgccagg gtgggcattc
    27661 cccagttaat cagttgcccg gcggtcatca gagcgttaac gtcgcgtccc tgacacagac
    27721 cgcgccactg cgcgagggct gattcgctac gaccatcgaa ccagttcagc caccacagag
    27781 tggcgttcag cggataatca cgcagcagtt tctcgcgctg caaacgcgcg ttgtcctgac
    27841 gaccacttaa caccagcagc agattatgca agcaaagcac ttcctgattg gttgggcagg
    27901 cacgaagact ttgttggcaa aaatccagac ccgcgtcgaa gttatcatta cgcgccgcca
    27961 gtcgtgccag accataatag ccgcctgctt tactattgcc gctccagacc gcacgccaga
    28021 aatcctcttc ggcttgttga tattgtccct gacgttcgta agcactggcg cgaatcaaac
    28081 ttgcttgtcc acactgcggt tttttgttca gcgcatgtgc acgtttcagc gcctgactgg
    28141 catacgccac cgcttgcggg aaatctgcgc ggttatattc cagcatcgcc agcgccaggt
    28201 tacagcgata atccagcggg tccagcgcca cgccgcgcag gtagtaatcg aacggtgaac
    28261 ggctggcgtg atgatattgc tccagatgct gaccgataaa ccaggcttca tctgtactgg
    28321 taatgtcttg tgctgccagt ggcgctttgg cgacgtccgg caacggcaac tcctgcggct
    28381 gatgttcctg ataactcagt acaatattgc cgtcggcatc ggagagttca atggtcagtc
    28441 tgtcaggatt gataccgtgc aacacgccct ggatggcggt cgctggtgtc agggccacgg
    28501 catcatcgag caacgcgttg catttgccga tttcgcggat cgccagacgg tatccgttca
    28561 acggagagat ggcatacagc ccccactcaa tcccccgctc actacgctgg agttttatca
    28621 ccgcatcgcg ggaggcattt tgcaccatgc ccagagaatg ataaggcagg aaatactgct
    28681 cgaaacgctt ctcttcgtaa gcatcaagcc aggtaaaatc aggctggtta tcggcaaaaa
    28741 taccggtcat cagttcgata tacgggccgt tattgtcggt cagactctta tcccacgcct
    28801 ggccaaattc actgtgtccc caactccact gttttttacc tggcgcaata tggtggttgg
    28861 caacgtgcag caaaccgcca tcttcatcgt gacaccacgc gccgacaaaa tcgtactgcg
    28921 atttttcggc catatatgag gttggaacag gcacattttt atagcgagaa atgtccactc
    28981 cggcggagta gtccactttg tagtaagtgc cggtggcgat ggggaaagcg gagacggccc
    29041 gtttgccgtg atcaaacacc gcagtcacat ccggtgggaa aacgctctga tgcccttcac
    29101 cccctttcac tgccgggttg acccaccaca agaaatgacg cggcgtggcg ttgccgttat
    29161 agacgcggct ggcgatttcc agtgccgccc ggtcagggcg cagggtgaaa cctgtcatca
    29221 cctgtaaacc atgcatcggc tccgtttcgc cgacccacac cgtctgtgca ccgtcttcat
    29281 gggcttcgag ggtgaaatca acgggcataa aggtggtcgg gcgatggtgt tgcggccagt
    29341 taaactcaat cccgccggag atccacggtc ccagcatccc caccagcgca ggtttaatga
    29401 cttcattgtg atagacaaaa tcgcgctgtt tcactttatc ccatgcgcga tgcacccgac
    29461 cgcccagttc cggcaggatc atcactttga tgtagtcgtt ttccagccac accgcctgct
    29521 aggatttcag ggttttctgc tcgctcagcg tatcggtcac gccgtaggga taaaccgcgc
    29581 cggacgatcc ctgataaacg cgattttcca ggaacatggg atgtatatcc tgcggcccgg
    29641 tttcataggt cgggatctca acgcgctctt gccacacttt tactggagtc atggtgccct
    29701 caatattaac aagacatact gaattaaaag atttgtggca gtgtattgaa caatctggca
    29761 atgttttcgc ggaataatca cgcaattaac taaacaaggt ttagtgaaga tgagagcctg
    29821 cattaacaat caacagattc gccaccataa caaatgcgtg attctggaac tgctgtaccg
    29881 gcaaaagcgc gccaataaat caacgctggc ccggctggcg caaatttcga ttccggcagt
    29941 cagtaatatt ttgcaggaac tggaaagcga aaaacgggtg gtgaatattg acgatgaaag
    30001 ccagacgcgc gggcatagta gcggtacatg gctgattgcg ccggaaggtg actggacgct
    30061 gtgcctgaac gtgacgccca ccagtattga gtgtcaggtt gctaatgctt gtttaagtcc
    30121 gaaaggcgaa tttgagtatt tacagattga tgtaccgacg ccgcaggcgc tgctgtccga
    30181 aatcgaaaaa tgctggcatc gccaccgtaa attgtggccg gaccgcacca tcaatctggc
    30241 gctggcaatc cacgggcagg ttgatccggt gaccggcgta tcgcaaacca tgccgcaagc
    30301 gccgtggaca acgccagttg aggtgaagta tctgctggaa gagaagctgg gcattcgggt
    30361 gctggtcgat aatgactgcg tgatgctggc gctggcggag aaatggcaaa ataattcgca
    30421 ggaacgggat ttctgcgtga tcaacgttga ttacggcatt ggctcgtcgt tcgtgattaa
    30481 cgagcaaatt tatcgcggca gtttgtatgg tagcggacag attggtcaca ccatcgttaa
    30541 tccggatgga gtcgtctgcg actgtggacg ttacggcagc ctggaaaccg tcgcctcgtt
    30601 aagtgcatta aaaaaacagg cgcgggtatg gctaaaatca caaccggtta atactcagct
    30661 tgagcctgaa aaactgacaa ccgcgcagtt aatcgctgcc tggcagagtg gagaaccgtg
    30721 gatcaccagc tgggttgatc gctctgccaa tgccattggt ttgagtctgt ataacttcct
    30781 caacatcctc aatattaatc agatttggtt gtacggtcgc agttgtgcct ttggtgagaa
    30841 ctggcttaat actattattc gccagacagg atttaacccg ttcgaccgcg acgaaggacc
    30901 gagcgtgaaa gcgacgcaaa ttggcttcgg gcaattaagc cgcgcacaac aggtgctggg
    30961 aattggctat ttgtatgttg aggcgcagtt acgacagatt tgatggcgcg ataacgtaga
    31021 aaggcttccc gaaggaagcc ttgatgatca taaacgaaaa attgcctgat gcgctacgct
    31081 tatcaggcct acacggagat tgcaatatat tgaatttgca agattttgta ggcctgagaa
    31141 ggtgttcatg ccgcatccaa catgaacaac gagcacattg tcagcaaatc accgtttcgc
    31201 ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
    31261 aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
    31321 ttcggcttct ttaaagccgc gacgggtaat cgccggagta cctacacgaa taccggaggt
    31381 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
    31441 gcccagagcg gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa
    31501 caggtggtta tcagtgccgc cggaaaccac tttgtagccg cgctcgagga acacttctac
    31561 catcgcttta gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag gctccatcgc
    31621 ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc
    31681 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaagat
    31741 caggccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
    31801 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
    31861 gaacagataa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc
    31921 ggaatatgca gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc
    31981 cagatcggcg taatcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag
    32041 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat
    32101 acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga
    32161 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
    32221 cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata
    32281 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
    32341 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
    32401 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
    32461 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
    32521 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
    32581 gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    32641 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    32701 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    32761 atgcaaaaaa ggaagaccat atgcttgacg ctcaaaccat cgctacagta aaagccacca
    32821 tccctttact ggtggaaacg gggccaaagt taaccgccca tttctacgac cgtatgttta
    32881 ctcataaccc agaactcaaa gaaattttta acatgagtaa ccagcgtaat ggcgatcaac
    32941 gtgaagccct gtttaacgct attgccgcct acgccagtaa tattgaaaac ctgcctgcgc
    33001 tgctgccagc ggtagaaaaa atcgcgcaga agcacaccag cttccagatc aaaccggaac
    33061 agtacaacat cgtcggtgaa cacctgttgg caacgctgga cgaaatgttc agcccggggc
    33121 aggaagtgct ggacgcgtgg ggtaaagcct atggtgtact ggctaatgta tttatcaatc
    33181 gcgaggcgga aatctataac gaaaacgcca gcaaagccgg tggttgggaa ggtactcgcg
    33241 atttccgcat tgtggctaaa acaccgcgca gcgcgcttat caccagcttc gaactggagc
    33301 cggtcgacgg tggcgcagtg gcagaatacc gtccggggca atatctcggc gtctggctga
    33361 agccggaagg tttcccgcat caggaaattc gtcagtactc tttgactcgc aaaccggatg
    33421 gcaaaggcta tcgtatcgcg gtgaaacgcg aagagggtgg gcaggtatcc aactggttgc
    33481 acaatcacgc caatgttggc gatgtcgtga aactggtcgc tccggcaggt gatttcttta
    33541 tggctgtcgc agatgacaca ccagtgacgt taatctctac cggtgttggt caaacgccaa
    33601 tgctggcaat gctcgacacg ctggcaaaag caggccacac agcacaagtg aactggttcc
    33661 atgcggcaga aaatggcgaa gttcacgcct ttgctgatga agttaaggaa ctggggcagt
    33721 cactgccgcg ctttaccgcg cacacctggt atcgtcagcc gagcgaagcc gatcgcgcta
    33781 aaggtcagtt tgatagcgaa ggtctgatgg atttgagcaa actggaaggt gcgttcagcg
    33841 atccgacaat gcagttctat ctctgcggcc cggttggctt catgcagttt accgcgaaac
    33901 agttagtgga tctgggcgtg aagcaggaaa acattcatta cgaatgcttt ggcccgcata
    33961 aggtgctgta atttgatgtt gccggatgga aacatccggc aacccttgac gcggtttaaa
    34021 ttgccgcgtc gtcctcttca ccggtacgga tgcgaatgac ccgagccacg tcaaagacga
    34081 agattttacc gtcaccgatt ttgccggttt gcgccgtgcg aataatggta tcgacacagg
    34141 tatcgacaat gtcgtccggt acgacaatct caattttcac tttcggcaga aaatccacca
    34201 tatactccgc gccgcggtac agctcggtat ggcctttctg gcgaccaaag cctttcactt
    34261 cggtcaccgt catgccggta ataccgactt cggccagtgc ttcgcggaca tcgtccagct
    34321 tgaagggttt tataatcgca tcaatctttt tcatgctatt ccttgaaaag gttgcctgtc
    34381 ttttgatctg ctaaacgtaa cacataacgc caattcattc cttgaaatcg tttgcatcca
    34441 gctcgtgtcg ggaaagcagt ttataaaatt ctgtccggtt gcgccccgcc attctcgccg
    34501 cgtgggtgac gttgcctttg gtgatttgca gcagcttacg caagtagttg agttcaaaat
    34561 gattacgtgc ctcaacaaag gttggcagcg ccgtattttc accctccagc gcctgctcca
    34621 ccagcgcatc actaatcacc ggagatgagg tcagcgccac gcactgttca atcacgttga
    34681 ccaactggcg cacattaccc ggccagctcg cggtcatcag gcgtttcatc gcatcggtag
    34741 agaacgcgcg gacaaacggt ttatgtcgct ctgccgcctg gcgcaacagg tgatttgcca
    34801 gtagcggaat gtcttctgtg cgctccgcca gtgccggaat tttcaggctg acaacgttga
    34861 ggcggtaata gagatcttcg cggaattccc cgcgcgccat cgcttttgac agatcacggt
    34921 gagtggcaga aataatccgc acattgatat caatatcgcg gttactgccc agcgggcgca
    34981 ctttacgctc ctgcaacacg cgcagcagtt tgacctgtaa cggcgcaggc atatcgccaa
    35041 tttcatcgag aaatagcgtt ccgccttccg ccgcctggaa taaaccttcg cgattgctga
    35101 cagcgccagt aaacgcgcca cgcgcatgac caaacagctc cgactccagc aattgctcgg
    35161 gcaatgcgcc acagttaata gcaataaatg gtttgctgtt gcgcgggctg gcgttgtgga
    35221 tagcctgggc gaaaatctct ttcccggtgc cgctctgacc gttaatcaaa acgctgacgt
    35281 ctgattgcgc caccagccgc gcctgttcca gcaaacgcag catcagcggg ctgcgggtga
    35341 caattgcctc gcgccagcgt tcatcggtgg ctggcgcgga ttgctccagc gcatcgtcaa
    35401 ttgcctgata tagcgcatct ttatcgacag gcttggtgag gaaactaaaa acgccctgct
    35461 gtgttgcagc aacggcatcg gggatagaac catgcgcagt aagaataatt actggcattc
    35521 ccggctgcac tttctggatt tcagcaaata gctgcatacc gtccatttca tccatccgca
    35581 ggtcgctgat aactaaatct actttttcgc gattcagtac ccgtaatcct tcagcgccac
    35641 tttccgccgt gaccacactg tagccttcgc tggtcaggcg caggccaagc agtttcagca
    35701 atcccggatc gtcatcgacc aataataaat gcgcaggttt atggctcatc aggagtgacc
    35761 tcatgggtgg atggcgcggg cttttcactc tcgtgcggcg tatccggcga gaaatttcca
    35821 gccggtttgc gggtcgagag ctggcgttca atatcggtca ggttttccag cttgcgggtg
    35881 gtgagttcca gttgctgctg tagaacgtga tgttgctggc gcaatgtatc cagctcgctg
    35941 tcgctggact gctggagttt actgtaacgt tggcgctctt ccgccagttg cagttgcagc
    36001 gcctgaccgt cgcgccagag ttgatacagt gggcgaacct gtgccgggat ctcggtactt
    36061 aacgcttcaa tacgcgcgac cagctggcgg cgctcatacg gcgtaatttt ggcgtcggcg
    36121 agcaaaatcc cttgtttaaa ggtattttgc cagctgccgt cgtcatattg gcgagcttgc
    36181 tgacgcgact gcgcaggcat taaacgatca gcacaatcca tcgcccgcag ccagtaaagc
    36241 ggattggttt cggttgattt accttgcagc gcccagatgt cgctacattc agtagaaaga
    36301 tagtcagcca gttgataaac cggaattttt tcttctgctg gcgtatcaat ggctggctta
    36361 ttgtgattct gcacgcaacc cagcaatgcc agacatggca gccctgccag ccacagccgt
    36421 cggggcaata atcgttgaaa aatgtgtcgc atattcacca gacttaaagc ctatcccagt
    36481 gggcgtaatt gttgcagaca gtctggacat ggactgcgcg gagaaaccgg ggcgtacata
    36541 tcgtacgtga agatttcgag cactacccgg ggccgaactg acaaataaaa tagcctgata
    36601 ggataggctg ttagcattat ttcgtgtttt tcgacgacgg taattcaatg cggaaacaaa
    36661 cgtcttgccc gctctcgtcg accagataca gttccccttg catacggcga atacaatccc
    36721 tggcaatgct taatcccaga ccgctgccct tcaccgcccc ttttcgctgg tggcttccct
    36781 gaaaaaaggg ttcgaagatc atggcgcgtt cctcttgcgg aatgggcgtg cctgtattga
    36841 tgacatcaat ataaacccgc gcaccatgta aactgctgcg aaggcaaatg ttaccggatt
    36901 cagccccgta gtgcaccgca ttggagtaaa gattatccag tacgctcatc agcagcattg
    36961 gctccgccag gcaagctgtt gctttgagat cgacgtcggt atgcatcatt ttagcccgtg
    37021 cgggcaggct atgagcagaa accactgtct ccaccagcgg tgctaattca acattctcca
    37081 gttccaccgc actgtccgcc tgtttacggt tgtaatcaag cagttgttcg atcagttttt
    37141 gcaaattgca gctgctgcta tcaagaatgc tcaccacctc tttttgctct ggcgtaagcg
    37201 gcccgacaac ctgatcagcc agtaattcag tgccctcgcg catactcgcc agtggcgttt
    37261 ttaattcatg agataaatgt cttaaaaatt gatggcgttg ggattccagc catgacaggc
    37321 gctcacttaa ccaaagaata cgttgcccaa ccgagcgtaa ctcgctcggt ccacagaacg
    37381 aaacgctatt gcccagagaa cgcccttccc ccagacggtt gatcatgcgc tcgatatttt
    37441 tcaccggccc gataatcatc cgcgtgaaaa gtagcaccat caccagactc accagaaata
    37501 gcaccagcga ttgccaacca aaatattgcc cacgttcggc gatttcacgc tgaagttgct
    37561 gcccacgaga gaacaccact gtgcgcgtgg cctgcaccat ttcggtattg gcactggcaa
    37621 aggcttcaag acgcgcggcg gcggcagcat cgggaccact gttgttacac tgaagttggg
    37681 ccagattgtt caaatcctga cgcaatgcct ggtaaagttt atcgtctggc agtacgcctg
    37741 cgtgggcatc gagcatttcg ctgtaacgct tgcgctggct ttgataaacc ttcgcaagcg
    37801 tagggtcgtc cagcacgcaa tactgacggt agctacgctc catctccagc gccgcgttgg
    37861 tcatcgcttc actacgccgg gcatcaataa gcgtagtgcg gttaaccagc gccgcctgat
    37921 cgctaagcgc attcaggctt tgccaggctt gccatgccag caccaacagg ggtagtagaa
    37981 ttagcaaaaa tgccagcatt accagttgtc gtaatgagcg gggaaaaacg ggccagcgtt
    38041 tcaaggtgtt actctcgtca gacgcgaata gcctgatgct aaccgagggg aagttcagat
    38101 acaacaaagc cgggaattac ccggctttgt tatggaataa ggcggtgcct aactcgacgt
    38161 ttcgcccgat ggttgatata gctacgctga tatcagaagt tggacggcag gcaccttgtt
    38221 gtgcgtcatt cgaattttat gtagcacgtc ccgaaggggc tgacataagt cggtgaatga
    38281 gccactgctt actattatgc agtaactgtg ccaataaaga aaatagtttg gtaacgtact
    38341 gattatacgt tgctttgagg gatttatggc ttcttcgcta tttgaatgta aatcagccac
    38401 tgtgtcgcta aaaagagaca acttaagata aacttattag attgcaataa aatcaatcat
    38461 ttaagtgtcg ccagaaagcg acacggtaaa ccacccattg tcgtgattta cagacacaaa
    38521 aaagctcccg gagttgggag cttatgatag tggttggtgc ttaatgccgc atccgggctg
    38581 caaaaccaat gaactgatga cttaccccaa ctgcttacgc gcattgcgga aaatgcgcat
    38641 ccatgggcca tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac
    38701 acgttccggg tgcggcatca taatggtgac tcgaccactt tcagtcgtaa ccgccgtaat
    38761 accgttcggt gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc
    38821 gacatagcgc agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg
    38881 cacttccacg cgcccttcac catgagagac agcaatcggc atttgcgagc ccaccatccc
    38941 ctgcaacagc agagacgggc tttgggttac ttcaaccagg ctgaaacgcg cttcaaagcg
    39001 atcggaggtg ttacgcacaa aacgtggcca caactcactc cccgggatca gttcacgcag
    39061 gttagacatc atctggcaac cgttacatac ccccagggcc agcgtttgcg gacgatggaa
    39121 gaaggttgca aactcatcgc gtacacggtc attgaacagg atagacttcg cccaaccttc
    39181 accggcaccc agcacatcac cgtaggagaa acctccgcac gcgaccaggg cgtgaaaatc
    39241 ctccagtccc gtacgtccag tcagcaggtc gctcatatgc acgtcgatag catcaaagcc
    39301 cccacgttgg aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagtac
    39361 ggcaactttc ggacgtgcgc cagtggcaat atacggtgct gccacatctt cgttgatatc
    39421 gaacgacagt tttacattca ggcccggatc ggcgtcgtta gatttcgcct gatgctcctg
    39481 atcggcacac tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca
    39541 gacacgcaac gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa
    39601 acggtcaccg gaaaccgcct gcccaacata atggacgcaa tccgcaagcc catgctgtgc
    39661 cagtacggac tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg cacccagttc
    39721 ttcgttaaac aacgccgcca ggcgatcgtc acccagagag gcgatatccg cgtcaatgcc
    39781 acaatgacct gcaaaggcca tttccgccag cgttaccagc aggcctccat cagagcggtc
    39841 gtgatacgcc agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt
    39901 cagttggaca acatcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc
    39961 cagcgccgtt gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt
    40021 atcttcggta caaagctgcg gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc
    40081 agaaatcacc agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg
    40141 ggttttcatc gacatagagt ctttacccac cgggatcgtc aggcccagcg ccggacaaag
    40201 ttcttcaccc acggctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc
    40261 cgccgccatc cagttggcgg aaagtttaat gcgtttgata tcgccaatct gtgttgcggc
    40321 aatattggtt aacgcttcac cgaccgccag acgggcagag gcggcgaaat ccagcagcgc
    40381 aaccggcgca cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt
    40441 agtgaccgcg cagttagcga ccggcacctg ccacggcccc accatctgat cgcgcgccac
    40501 catgccggtt acgctgcggt caccgatggt caccaggaaa gttttctccg ccacggtcgg
    40561 cagatgcagt acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga ccagcgcatc
    40621 gcccttcgct ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac caagcaggac
    40681 gtccagcggc agatcgatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag
    40741 ttcttcggtc gcttcaccaa tcaccgcgta gggtgcgcgc tcacgcttac acagttcgtc
    40801 aaacagcggt aattgatcgg cagcaaccgc cagcacgtag cgttcctggg attcgttaca
    40861 ccagatttcc agcgggctca tgcccggttc gtcgcttaga atatcgcgca gttcaaattt
    40921 accgccgcgt ccgccgtcgc tcaccagctc cggcatcgcg ttagaaagac cgcccgcacc
    40981 gacgtcgtgg ataaacagaa ttgggttagc atcaccaagc tgccagcaac ggtcgatcac
    41041 ttcctggcaa cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc
    41101 ggcatcagac tgaccagacg ccatggaaga cgccgcgccg ccgccaagac cgatgttcat
    41161 tgccgggccg ccgagaacga ccagcttcgc accgacgttg atctcgcctt tttgtacgtg
    41221 atcggcgcga atgttgccga tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag
    41281 ctcttcgcca ttgtggctgt tcactttttc ttcataagta cggaagtagc cgttcagtgc
    41341 cggacgacca aattcgttgt taaacgccgc accgcccagc gggccttcgg tcatgatgtc
    41401 cagcgcggta acaatgcgct caggcttacc gaaatcttct tcccacggct gttcgaagcc
    41461 aggaattcgc aggttggata cggagaaacc aaccaaacca gctttcggct tcgcgccgcg
    41521 cccggtggca ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc ccggccacgg
    41581 agaaatcgcc gtcgggtggt tgtgagtttc gactttcatc aggatgtgcg ccggttcctg
    41641 atggaaattg tagcggcccg tttcgtggtc agcaaagtag cggcccactt cagaaccttc
    41701 cattacggcg gcgttatctt tataagcaga gagaacgtga tctggcgtgg tttcgaaagt
    41761 atttttgatc atcctgaaca gcgatttcgg ctgttgttca ccgtcgataa tccagtcggc
    41821 gttaaaaatt ttgtggcggc agtgctcaga gttcgcctga gcgaacatat acagctcgat
    41881 gtcgttcggg ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa tttcatcttc
    41941 cgccagagcc aagccaagac gcaggttagc gtcgatcagc gcctgacggc cctgccccag
    42001 caaatcaacg cttgtgaccg gagtcggttg atggtgggca aacaactgct ctgcatcatc
    42061 taaatcaaaa aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa cctgctgcca
    42121 ttgttcattg gtcagcgtac cggcttctat atagtaagca acgccgcgct caaggcggtt
    42181 tacctgttgt agcccgcagt tatgggcaat atcggtcgct ttcgaagacc agggagagat
    42241 ggtgccagga cgcggggtca ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc
    42301 cggaccatat ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta acggcgcatt
    42361 gaggtcagca aaatggacat actcggcgta aatattgtga accgggagcc tggcagcctg
    42421 aaaacgtgcc agcagtttgt tgattcggaa tgccgacagt gcaggcgaac cacgcagaat
    42481 ttccatcata agtctctcgt cttctaagct ttcggtgtac ccaagggggg ggaaacgggc
    42541 gtcattataa agaatctgat gcgctgacga aaccgtttgc gtggaaataa aatcaccatc
    42601 gtgaattagc aacgcgtgcc gccaatggct gtaataagtt gccatctggc gcaggtttac
    42661 gcaaaatgcc gctcatttat gagtaaacct ttcactatta ttacgttttt tcaagctggg
    42721 acgcgcacga cacagagaat taactaattg aaaaaattaa agattaatta tctgttcatc
    42781 ggcattctgg cactgctgct cgcggtcgct ctctggccat ccattccctg gtttggtaaa
    42841 gccgacaacc gtatcgccgc cattcaagcg cggggagagt tgcgtgtgag caccattcat
    42901 actcccctga cttataacga aatcaacggg aaaccttttg gcctggatta cgaactggcg
    42961 aaacagtttg ccgattacct cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc
    43021 cagctgtttg acgaccttga taatggtaac gccgacctgc tggcggcagg actggtctat
    43081 aacagtgagc gggtaaaaaa ttatcagcct ggccctacct attattccgt gtcacaacaa
    43141 ctggtttata aagtgggtca gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa
    43201 ctcaccgttg caccgggtca tgtggtagtt aacgatctcc agaccctgaa agaaacaaaa
    43261 ttcccggaat taagctggaa ggtagacgac aaaaaaggct ctgcggaatt aatggaagat
    43321 gtcatcgaag gaaaactcga ttacaccatt gctgattctg tcgccatcag cctgtttcag
    43381 cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg atgaacaacc ggtgacttgg
    43441 tttagcccgt tagatggcga taataccctt tccgccgccc tgctcgactt cttcaacgaa
    43501 atgaatgaag acggtacgct ggcacgcatt gaagagaaat acctggggca tggcgatgat
    43561 tttgattacg tcgatacgcg cacattttta cgcgccgtcg atgcggtact gccgcagtta
    43621 aagcccctgt ttgagaaata cgccgaagaa attgactggc gtttgctggc cgctattgct
    43681 tatcaggaat cgcactggga tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg
    43741 atgttaacca aaaataccgc gcaaagcctc ggcattacgg atcgtaccga tgccgaacag
    43801 agcatcagcg gtggcgtgcg ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg
    43861 ccggagaacg aacggatctg gtttgccctc gctgcgtaca atatgggcta tgcgcatatg
    43921 ctggatgccc gcgccctgac ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta
    43981 aaacagcgtc tgcctttact tagccagaaa ccctattaca gcaagctgac ttacggctac
    44041 gctcgtgggc atgaagccta cgcttatgtc gaaaatattc gtaagtatca gattagcctg
    44101 gtgggttatc tgcaagagaa agagaagcag gctacagaag cggcgatgca actggcgcag
    44161 gattatccgg cggtatcgcc tacggagttg ggcaaagaga aatttccttt tctctcgttt
    44221 ctttcccagt cgtcatcaaa ctatttgacc cattctccct ctctgctgtt ttccaggaaa
    44281 gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg cttttttctg cgctttaatt
    44341 tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg cgcactcatc cgccagtatt
    44401 ccttccgtaa tttccactcg gtgattcata cccggatgat gcagcacatc cattaaagat
    44461 cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg
    44521 atcatcgctc cggcacacat tacacatggt tcaagcgtga catacaacgt ggcgtcgatc
    44581 agacgataat tttgcatcac cagaccaccc tgccgcaggg ccatgatttc tgcatgtgcg
    44641 gtgggatcat ggcgaccaat cgggcggttc cagccttcgc cgattacccg attgttatgc
    44701 actaataccg cgccgaccgg cacttcccgc tcatcccagg cacgtttcgc cagcgtcagc
    44761 gcgtgacgca tccaatattc gtggctaaat tcgacttcag acaaaaagaa aactccggtt
    44821 ataaaagcgc ggcgcattat acacggacgc tatgctttac tctattccag ttgctggagt
    44881 tcaccgcgcg gggttactcg ccaacgatgc tgacagaaat aaagcagcgg attgtcctga
    44941 ttactgtcgc tatagccact gtacagccgc agcggcgtgc cgattttgcg ctccagttgt
    45001 gcgacctttt catgtcccag acaacgcatc gtcaataccc aaccaccata gccacgctga
    45061 atttggctgg cgataagatt aacccgcggc agccagggcg tatcgaaata aaccgcttca
    45121 accagcggct gcggagagcc ggtaatcaac cagatatcag catcggaact taacaggtag
    45181 gtggttaatc gctcctgaac cagcggaaag gcggtaacat tgtcgcgaaa ccagcgcacg
    45241 aaatcggcct gcaacgtctg taaacgtgct tcgctgtgac caaaagtgca cccccacaga
    45301 agcagactca tcggccagcg tgccgcacga ccttttatca ataacgcaat ggctataatc
    45361 ggtaacaacg gcaggacaag taacgcattc agcggttggc gacgcagtaa atagcgcaga
    45421 aaactgccga acatatcctg ctgatgcaat gttccatcta agtcaaaaaa caccacacga
    45481 cgctcgtgag ttgccaaacc atactcctct ggactgaaac atcctgatta attttcgcta
    45541 ctacatagcc taacagatag atcatcactt ttccggcaac aatcgaccgc atcatggcta
    45601 actggaattt ttaattcatg gcaattagcg gcaatggaat ataaaattca ctcgcgtgtg
    45661 tctcatattg cctgttgtcg ccactatttc atcgcgagga aagatatgaa cggcttactt
    45721 cgtatccgtc agcgttacca agggcttgcc cagagtgata aaaaactggc ggattatctg
    45781 ctgctacaac ctgatacggc gcgccattta agctctcagc aactggccaa cgaagccgga
    45841 gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg gctataaagg ttttccggcg
    45901 cttaagttgg cgttgagtga agcgctggca agccagccgg aatcgccctc cgtgcccatt
    45961 cataaccaaa tccgcggtga tgatccgtta cggctggtcg gcgaaaaact gattaaagaa
    46021 aataccgccg ctatgtacgc aacgctaaac gttaatagtg aagagaaact gcatgaatgc
    46081 gtaacaatgt tgcgctctgc gcggcggata attctgaccg gtattggcgc ttcgggtctg
    46141 gtggcgcaaa actttgcctg gaagctgatg aagattggct tcaatgctgc cgcactgcgc
    46201 gatatgcatg cgctactcgc aacagtacag gcgtcgtccc ctgacgatct gttattagcc
    46261 atttcctaca ccggtgtacg acgcgagtta aacctggcgg cagatgagat gctgcgagtg
    46321 ggcggaaaag tgctggcgat taccggcttt actccgaatg ccctgcaaca gcgtgcttct
    46381 cattgcctgt ataccattgc cgaagaacaa gcgacaaaca gtgcttcaat ctctgcttgt
    46441 cacgctcagg gaatgttaac ggatttgctg ttcattgcgc tgattcagca ggatctggaa
    46501 ctggcaccag aacgtattcg tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag
    46561 aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag agtttcctta tggcgctgtt
    46621 aatcactaaa aaatgcatca attgtgatat gtgtgaaatc aaataaaaac acatttattt
    46681 tcatagtgat acagcaatgt gttattgaat gtgttgttgg atttatggtg caaaaaaatt
    46741 ttgcataagt tcagcaacac aaagaacacc actttcttta acgtcctccg cgccctaaag
    46801 gacggggctt tacggcgcaa aaggcctgtg acatacaaca ttaacttttc agtcaaccgt
    46861 agcacgttct tgtatacgcc gtgtctgcgg ttttatataa ccctggctgg aaacctctta
    46921 tacaaagtcg attcgccaac atcatagatg atcgccacct tctggcgaga aactcctgat
    46981 gcaattagtc gcccggcctg cgcccattat tctggtgaaa gtttgggacg gcgaccgcca
    47041 attcgtccct gtgcgcgagc agcttccagt cctgcttttg ttcgttcaac aatcagttca
    47101 cgttccattt cagccagggc acccatcaca tggaagaaaa agcgtcccat tggtgtgctg
    47161 gtatcaattg aatcagtcag actacgaaag ttgatgcctc gttcgcgcaa cgcctccacc
    47221 agcacgacaa gatgccgcat actgcgcccc agccgatcca gcttccagac aaccagtgtg
    47281 tcgcctgccg ataatgtctt gagcagtttt ttcagccccg gtctttcgga ctttgtacca
    47341 cttatcttgt cttcaaaaat cagctcgcat cctgcacagt tcaacgcatt acgttgtaga
    47401 tcggtattct ggtcatttgt tga
//
LOCUS       DAJTRN010000022        44921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRN010000022 DAJTRN010000000
VERSION     DAJTRN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            9..2162
                     /locus_tag="OO328_001267"
     CDS             9..2162
                     /locus_tag="OO328_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5929897.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2215..3945
                     /locus_tag="OO328_001268"
     CDS             2215..3945
                     /locus_tag="OO328_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929898.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4005..4496)
                     /gene="yajQ"
                     /locus_tag="OO328_001269"
     CDS             complement(4005..4496)
                     /gene="yajQ"
                     /locus_tag="OO328_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5929899.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4664..5575
                     /gene="panE"
                     /locus_tag="OO328_001270"
     CDS             4664..5575
                     /gene="panE"
                     /locus_tag="OO328_001270"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5929900.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5538..6128
                     /gene="yajL"
                     /locus_tag="OO328_001271"
     CDS             5538..6128
                     /gene="yajL"
                     /locus_tag="OO328_001271"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5929901.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6182..7630)
                     /gene="thiI"
                     /locus_tag="OO328_001272"
     CDS             complement(6182..7630)
                     /gene="thiI"
                     /locus_tag="OO328_001272"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5929902.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7836..8078
                     /gene="xseB"
                     /locus_tag="OO328_001273"
     CDS             7836..8078
                     /gene="xseB"
                     /locus_tag="OO328_001273"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5929903.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8078..8977
                     /gene="ispA"
                     /locus_tag="OO328_001274"
     CDS             8078..8977
                     /gene="ispA"
                     /locus_tag="OO328_001274"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5929904.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9002..10864
                     /gene="dxs"
                     /locus_tag="OO328_001275"
     CDS             9002..10864
                     /gene="dxs"
                     /locus_tag="OO328_001275"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5929905.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10919..11836
                     /gene="yajO"
                     /locus_tag="OO328_001276"
     CDS             10919..11836
                     /gene="yajO"
                     /locus_tag="OO328_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5929906.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11947..12465)
                     /gene="pgpA"
                     /locus_tag="OO328_001277"
     CDS             complement(11947..12465)
                     /gene="pgpA"
                     /locus_tag="OO328_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5929907.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12443..13420)
                     /gene="thiL"
                     /locus_tag="OO328_001278"
     CDS             complement(12443..13420)
                     /gene="thiL"
                     /locus_tag="OO328_001278"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5929908.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13498..13917)
                     /gene="nusB"
                     /locus_tag="OO328_001279"
     CDS             complement(13498..13917)
                     /gene="nusB"
                     /locus_tag="OO328_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5929909.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13937..14407)
                     /gene="ribE"
                     /locus_tag="OO328_001280"
     CDS             complement(13937..14407)
                     /gene="ribE"
                     /locus_tag="OO328_001280"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5929910.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14496..15599)
                     /gene="ribD"
                     /locus_tag="OO328_001281"
     CDS             complement(14496..15599)
                     /gene="ribD"
                     /locus_tag="OO328_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5929911.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15603..16052)
                     /gene="nrdR"
                     /locus_tag="OO328_001282"
     CDS             complement(15603..16052)
                     /gene="nrdR"
                     /locus_tag="OO328_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5929912.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16203..16742
                     /locus_tag="OO328_001283"
     CDS             16203..16742
                     /locus_tag="OO328_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5929913.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17040..17924
                     /gene="tsx"
                     /locus_tag="OO328_001284"
     CDS             17040..17924
                     /gene="tsx"
                     /locus_tag="OO328_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5929914.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18101..18448)
                     /locus_tag="OO328_001285"
     CDS             complement(18101..18448)
                     /locus_tag="OO328_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5929915.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18577..19548)
                     /gene="secF"
                     /locus_tag="OO328_001286"
     CDS             complement(18577..19548)
                     /gene="secF"
                     /locus_tag="OO328_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5929916.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19559..21406)
                     /gene="secD"
                     /locus_tag="OO328_001287"
     CDS             complement(19559..21406)
                     /gene="secD"
                     /locus_tag="OO328_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5929917.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21434..21766)
                     /gene="yajC"
                     /locus_tag="OO328_001288"
     CDS             complement(21434..21766)
                     /gene="yajC"
                     /locus_tag="OO328_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5929918.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21789..22916)
                     /gene="tgt"
                     /locus_tag="OO328_001289"
     CDS             complement(21789..22916)
                     /gene="tgt"
                     /locus_tag="OO328_001289"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5929919.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22971..24041)
                     /gene="queA"
                     /locus_tag="OO328_001290"
     CDS             complement(22971..24041)
                     /gene="queA"
                     /locus_tag="OO328_001290"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5929920.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24134..24707
                     /gene="acpH"
                     /locus_tag="OO328_001291"
                     /pseudo
     CDS             24134..24707
                     /gene="acpH"
                     /locus_tag="OO328_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24712..26529)
                     /gene="malZ"
                     /locus_tag="OO328_001292"
     CDS             complement(24712..26529)
                     /gene="malZ"
                     /locus_tag="OO328_001292"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5929921.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26685..28058)
                     /gene="proY"
                     /locus_tag="OO328_001293"
     CDS             complement(26685..28058)
                     /gene="proY"
                     /locus_tag="OO328_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5929922.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28134..29453)
                     /gene="brnQ"
                     /locus_tag="OO328_001294"
     CDS             complement(28134..29453)
                     /gene="brnQ"
                     /locus_tag="OO328_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5929923.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29860..31155)
                     /gene="phoR"
                     /locus_tag="OO328_001295"
     CDS             complement(29860..31155)
                     /gene="phoR"
                     /locus_tag="OO328_001295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5929924.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31213..31902)
                     /gene="phoB"
                     /locus_tag="OO328_001296"
     CDS             complement(31213..31902)
                     /gene="phoB"
                     /locus_tag="OO328_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5929925.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32092..33294
                     /gene="sbcD"
                     /locus_tag="OO328_001297"
     CDS             32092..33294
                     /gene="sbcD"
                     /locus_tag="OO328_001297"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5929926.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33291..36434
                     /gene="sbcC"
                     /locus_tag="OO328_001298"
     CDS             33291..36434
                     /gene="sbcC"
                     /locus_tag="OO328_001298"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5929927.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36560..37741
                     /gene="araJ"
                     /locus_tag="OO328_001299"
     CDS             36560..37741
                     /gene="araJ"
                     /locus_tag="OO328_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5929928.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37884..38792)
                     /gene="mak"
                     /locus_tag="OO328_001300"
     CDS             complement(37884..38792)
                     /gene="mak"
                     /locus_tag="OO328_001300"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5929929.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38917..39828
                     /gene="rdgC"
                     /locus_tag="OO328_001301"
     CDS             38917..39828
                     /gene="rdgC"
                     /locus_tag="OO328_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5929930.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39906..40361)
                     /locus_tag="OO328_001302"
     CDS             complement(39906..40361)
                     /locus_tag="OO328_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929931.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40358..40693)
                     /locus_tag="OO328_001303"
                     /pseudo
     CDS             complement(40358..40693)
                     /locus_tag="OO328_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40848..41132)
                     /gene="ppnP"
                     /locus_tag="OO328_001304"
     CDS             complement(40848..41132)
                     /gene="ppnP"
                     /locus_tag="OO328_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5929932.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41204..41881)
                     /locus_tag="OO328_001305"
     CDS             complement(41204..41881)
                     /locus_tag="OO328_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5929933.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42139..42330)
                     /gene="yaiA"
                     /locus_tag="OO328_001306"
     CDS             complement(42139..42330)
                     /gene="yaiA"
                     /locus_tag="OO328_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5929934.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42380..42904)
                     /gene="aroL"
                     /locus_tag="OO328_001307"
     CDS             complement(42380..42904)
                     /gene="aroL"
                     /locus_tag="OO328_001307"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5929935.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43087..43545)
                     /locus_tag="OO328_001308"
     CDS             complement(43087..43545)
                     /locus_tag="OO328_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5929936.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43665..44474
                     /gene="proC"
                     /locus_tag="OO328_001309"
     CDS             43665..44474
                     /gene="proC"
                     /locus_tag="OO328_001309"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5929937.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44491..>44921)
                     /locus_tag="OO328_001310"
     CDS             complement(44491..>44921)
                     /locus_tag="OO328_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5929938.1"
                     /translation="NEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTR
                     QLQITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRI
                     SVGVAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11661 a        11709 c        10996 g        10555 t
ORIGIN      
        1 aatacatcat gtacaaatca ttatttacta ttatcattct actgtatagc gccagttcat
       61 atgcaaaaag cgaatcccct cccgcaagca ctataacttt cattgcacaa atcgatcatg
      121 ttgagctaaa taaaacagca ttatctcatg atatgaaact cgttcttgcc gatcgttttt
      181 atttcaaaac cagtaatcct tgcgaaacaa attcaatttc tgctcgacca gaatcaatgg
      241 atattactga caaagcctat gccgacaaat taaaaaaatt agaaactcct tttcttgatg
      301 atcgctattt ctttatgacc atcaatcagt gtgacggtaa tggcactccc atgctgacag
      361 acataaaagt atgtacaaca gaattatgtg gtgctgaata tacaaagaaa atgtctactg
      421 tatggctcga tgaaacttta aaaccaactg ctaaacgtct ggcaatttat tttattcaat
      481 ctcctttacc ctatgacgag gagaaaaaac aatggaaagt caccggatgg tatatctcat
      541 caactgataa tcagtcttca actaaacaga ttgcatttga aggatataca gacacagata
      601 acctctccga ttctaaattt atttcttcat ttaaatcctg gtttgaaagt ggaaagataa
      661 aaaacattgc taactataat ccaaaaggag aaagagatgg aaaatacgaa agttattaca
      721 atagcaatgg taacgtctat gaatctctaa gtttttctaa tggtcttatc aacggtgagt
      781 ttattacata ttatgaaaat ggggccattg aaagtaaacg tcattttaat aacgacaaaa
      841 ttaaagatgg tgaatgccct catttttatg aagatgggaa aatcaaacag caacatagct
      901 atctgaatag taaactcgat ggtcctgcat atgaatatta tcctgatggc aaactcatgc
      961 aggaaaattt ctatcagcaa agtgtgttga ttggaaacga taccagttac taccaatccg
     1021 gaaaaataag ctctatacac aatagaaatt cccgtggtca gtatgatgga gctaacgaac
     1081 gttatagtga agagggtaag ttactgtcta aatccgtcta taaagacgga aaacaaattt
     1141 ctgttcaaac ctggtatgaa aatgggcaga aagaagagga gaaacatttt gatgagcaag
     1201 gccaattaaa tggtctggtt aagcaatggt ataaaaatgg taatcttgca aaatcacaaa
     1261 actataaaca tgacatactc gatggtaaca gtgaagagtg gtacgaaaat ggcatccctg
     1321 aaagtttgta cccttataag aatggaaaaa ccgacggcat tgccaaatca tggaataagt
     1381 atggtaaatt aacctattca atcgaataca aaaatggcgt agagaatggg gcatatcgta
     1441 actggagcaa aaacacaggt aaactaacca aagaaaccct gtacgtcaat ggtatcagac
     1501 agggagtgga aaaagagttt aacgatcgca caggtaaact attaacctcc acgcaatatg
     1561 ttaataataa aagacatggt acggaggaaa cttatgatca aaacggaata aaatatatta
     1621 catgctatca aaatgatcag aaattatcgt ctttagacaa tccaacacaa ataaaagata
     1681 atgcaacaac aggcgattcc agtgctcaat ttgcattagg gaaatacgaa ttcatctgtg
     1741 caaatataga tgaagggatt aaatggttaa ccaaatctgc agagcaaaaa aacactgatg
     1801 cgatatactt tttagctaca gcctacaaag gcaatggtat acctgcaaac aatgaaaaat
     1861 acctagcata tctgcaacaa gctgcaacac tgggcagtag taatgcccag gcggaaattg
     1921 gctatttata tctcatcgga aaagaactac cacagaatct tcctgatgct ggtgtctggt
     1981 tcaaaaaagc agctgcacaa ggtaattttg ccgcacattt ctatctggga aggatgtatc
     2041 agaatggtga cggcgttgag agaaatatgg aaaaagccag gtttcatcta tctaatgcgg
     2101 cagaaggtgg aatagaacct gcgcttaaag cattaaatga acttgaacat caaacaaaat
     2161 aaagcgctca gggaccacgt aaggttcatc aaacagaaaa ggatatagtt tgcgatgaaa
     2221 gcgaaatcca ctttgttatt aatgatttta tctgtttttt attctcatca ggttttatcc
     2281 agcgaaccca ctcagccgga tgtttattct gtcgtcgaga aaaaactaaa ctctgcttta
     2341 cctctggaag aaaataccac cttcagatcg caggctgaat ggtttaattt gcaatatgaa
     2401 ttattagtat ctggctaccc tgaacgtgct tacaatttac tgagcaaact tgagacagaa
     2461 tcgaaaaacg ctaaatatta tctggattta actgcctcca gagtcgctga agaagaagca
     2521 ccagagacag ccttaacttt tttatcaaaa attgggttaa ataaaccggc tactcttaca
     2581 tcctttgaga gctatatcaa tagctggata aaaagcaaac aacccgaaaa ggctatgcag
     2641 cttctcagcc tcgatgagaa tgccctgaat tattatttac cgactgtgtt agtcgcatat
     2701 cagatcgttc ctgataaggc aataagtatt tataattctg tatatggaga taatgttgtt
     2761 ataccctatc agcaagtcac catgttactt acagttgcgg agggttatca aacaaaaggc
     2821 gatataaaaa acgccattca ttatgccgat aaggcgctga atatgtttga taaagctatt
     2881 gctgtagata gcagtcgtga atcccattat agtgatgagt atttgaaact catgaatatc
     2941 tacgccgcca atggcaacaa agaaaaagcg gtggttttat ctcaacgtct acacaaagcc
     3001 attaccgaat ctcaatcgaa ttacttaagt actctggaag gaattttatt gttttataga
     3061 aataatgata tgcagcaaga ctatcaaaac agcctttcaa attacatagt atttatcgat
     3121 aaaatttttg ccttttcccc atcaacaaga agtgaattaa gcctcattaa tttattaaat
     3181 tcattaaatg aggtggaatt gatgagaaaa cgtctggcgt ttctgatgac agcaccagaa
     3241 tacgcttgtt ataatagtca gtattgttat gaagacaaag tcagcgcatt aaaaatcctg
     3301 tatcgtcatc atgacaatga gctaatggac aaatatttca cgactcttct taaagagatc
     3361 agtaatcaac cattagaaga ctgggatata ataataagta atactggtga aaaatttagt
     3421 gaagtgggtc taaccaaata tgctcaacat ctcgcatctt tcgctgaaga attttatata
     3481 aaagagcaaa aatcacattc tgaaagtgaa atgagaggac tattttcatc acttgccgaa
     3541 ttatatagtt ttggtaatga tactgccagc gcaaaccgag tgttccatca gcatgtccct
     3601 gccttcaata cagatcatat gattcaatat tttataaatg ccaaagaatg gaacaatgcc
     3661 agggaattat tgataaaaga ggaaatgttg ggtatgcata atctgatatt gttggagaat
     3721 atttgtatgc aaaaaaatgc agaatgcatg gcacacatta cctttacgct taataaatta
     3781 acaacacaac cagcaataac aaaaatagat agcgtgggta atgagcaact ttatcagatt
     3841 ggcaaaatct accataagtt ggaaattaaa cctgagccag agcaacaggt tttaattcag
     3901 tcgctttatg accgggcatc aggaagcgcc tctgcaactc aataaatgca tggtcaaaag
     3961 ggccaccacg gtggccctga caatcagtag ctatatccag aaagttaatc gcggaagttt
     4021 ttgaactgga acggctgacc gagatcgcca ccgcgcacca tcgccatgac agcctgcaaa
     4081 tcatcacgag atttgccagt cacgcggatc tcatcgccct gaatttgcgc ctgcactttc
     4141 agtttgctgt ctttgatcat cttgacgatt tttttctgag tggcgctctc aatgccctgt
     4201 ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattttc cggtacatcc
     4261 agtgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc cagcaactga
     4321 ttgacctgga agtcggactc gctcaacact ttgatggttt tgctggcgtc gttcagctca
     4381 aatgaggctt caacgttacg gaagtcaaaa cgggactcca cttcgcgact cgcgttatcg
     4441 accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga tggcatcttt
     4501 tcctctccct tcatttttga tgcgaagcat aatacctgca aagttaaata accaacagtc
     4561 ctgcaccagg acgtatccct taattgtgca tagtcgcaac agcatgcaca attaagggat
     4621 agccttattg aacacccgga gtggttgcgg gtgaggagga acaatgaaaa ttaccgtatt
     4681 gggatgcggt gccttagggc aattatggct tacagcactt tgtaaacagg gtcatgaagt
     4741 tcagggctgg ctgcgtgtac cgcaacctta ttgtagtgtg aatctggttg agacagatgg
     4801 ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttctcgcca ccagcgatct
     4861 gctcctggtg acgctgaaag catggcaggt ttccgatgcc gtcaaaagcc tcgcatccac
     4921 actgcctgta actacgccaa tactgttaat tcacaacggc atgggcacca tcgaagaatt
     4981 gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc gccgcgacgg
     5041 caatgtcatt attcatgtgg caaacggaat cacgcatatc ggtccggcac ggcaacagga
     5101 tggcgattac agttatctgg cggatatttt gcaaaccgtg ttgcctgacg ttgcctggca
     5161 taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgcgtga ttaatccact
     5221 gactgccatc tggaattgcc cgaacggtga attacgtcat catccgcaag aaattatgca
     5281 aatatgcgaa gaagtcgcgg cagtgatcga acgcgaaggg catcatactt cagcggaaga
     5341 tttgcgtgat tacgtgatgc aggtgattga tgccacagca gaaaatatct cgtcgatgtt
     5401 gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt ttctcttacg
     5461 ccgcgcccgc gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg aaatggtaaa
     5521 aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct ggtagtgaag
     5581 agactgaagc cgtcaccacg atcaacctct tggttcgcgg cggtatcaaa gtcaccactg
     5641 ccagcgtcga cagcgatggc aacctggcaa ttacctgctc gcgcggcgtg aagctgctgg
     5701 cggatgcgcc gctggtcgaa gtggctgatg gcgaatatga cgtgatcgtg ctgcctggtg
     5761 gcattaaagg cacggagtgt tttcgcgata gcactctgct ggttgaaacc gttaaacagt
     5821 tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc gtgctggtgc
     5881 cgcacgatat ctttccgatt ggcaatatga ccggcttccc gacgctgaaa gacaaaattc
     5941 ccgctgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa ttgctgacca
     6001 gccaggggcc gggtacagct atcgactttg gtctgaaaat tatcgacctg ctggttgggc
     6061 gcgagaaagc ccatgaagtg gcatcacaac tggtgatggc ggcagggatt tataattatt
     6121 acgagtagtg tcggatgcgg caaacgtcgc atctgaccag atgcgacgta aaaactacaa
     6181 attacgggcg atataccttc acattgttaa agccttgctc gcgcagatag agcgcctgca
     6241 gacggctcat caccccgcgc tcacaccaca gcagccaggt tttgttctgg tcgagatcgc
     6301 caaatttggt gctcagttta tagaacggca gagaaaccac atcaatccct tcgactttca
     6361 gtggcttatc ttcctgttca tcgacagaac ggatatcgag aatcacgtcg ttcgggccga
     6421 agccgttgac ggtttccact tccaccactt cctgctcggt ctgctgggcg atttcgcgga
     6481 tatcaacgtt attcgcttcc tcaaccactt tatcgagaat actgaagtcg aacttctctt
     6541 cttccgcttc aatcttcgat ttaaccgctt tcaccgtcgg gcttttggag atcacgccac
     6601 aatattccgg catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc aggttgatga
     6661 tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtgtcagag acgttatcaa
     6721 tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg ccggtgacca
     6781 gcgcctgtac gccgtaacgt tcagccactt tagatgcggc acgcaccatc atacgtttga
     6841 ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgaca accggttcga
     6901 aattaatggc gacaaaacgc acgcggtggg agctgccaaa gcggttccac agataatgcg
     6961 ccacctgacg cacgccaatt tcatgcgctg cgccgccgag gttaaagaag cagtaatgca
     7021 cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa ccaccggaaa
     7081 tgagcgacag cacatcttcc tgggtgccaa tcgggaaacc accaatacct tcgtagcgac
     7141 ctttaatcag caggaggcga tcgtcttcca cttccagatg aacagttaca tccgggttag
     7201 tcagcttcac gcgcgcggat tcaatatgct gatttaaacc gccgccgacg taacgttcca
     7261 catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag aaggttttgc
     7321 cttccagctg atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc atgtcggtaa
     7381 acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc agggcgtcgc
     7441 gaatagtcag acgctggttt tcatctttcg cgcgaacttc gatgttatcc cagtggcgga
     7501 cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg gtaaggattt
     7561 ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac aatttaatga
     7621 taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat agggaaaaat
     7681 acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa catcgctttg
     7741 ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc cgttaccatg
     7801 acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat gaggcgcccg
     7861 ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg gaaagtggcg
     7921 acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg gcacgtcagg
     7981 ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct gacaatgaag
     8041 acgcctctct aacccctttt acaccggaca atgagtaatg gactttccgc agcaactcga
     8101 agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca
     8161 gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg
     8221 acctttcctg gtttatgcca ccggtcatat gtttggcgtt agcacaaaca cgctggacgc
     8281 acccgctgct gccgtagagt gtatccacgc ttactcatta attcatgatg atttaccggc
     8341 gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt ttggcgaagc
     8401 aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattc taagcgatgc
     8461 caatatgccg gaagtgtcgg atcgcgacag aatttcgatg atttctgaac tggcgagcgc
     8521 cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca
     8581 cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc
     8641 cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc tgccagtact
     8701 cgacaagtac gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt
     8761 ggtaggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag
     8821 tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga
     8881 cgatgcccgt caggcgctga aacaactggc tgaacagtca ctcgatacct cggcactgga
     8941 agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta ataggcccct
     9001 gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt
     9061 acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact
     9121 cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac
     9181 cgtggcgctg cactatgtct acaacacccc atttgaccaa ttgatttggg atgtgggaca
     9241 tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca
     9301 gaaaggcggt ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt
     9361 cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg ccgagaaaga
     9421 aggcaaaaat cgccgcaccg tctgtgtgat tggcgacggc gcgattaccg ctggcatggc
     9481 gtttgaagcg atgaatcacg cgggcgatat tcgtcctgat atgctggtgg tcctcaacga
     9541 caatgaaatg tcgatttccg aaaatgttgg cgcgcttaac aaccatctgg ctcagctgct
     9601 ttccggtaag ctttactctt cactgcgcga aggcgggaaa aaagttttct ctggcgtgcc
     9661 gcccattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg tggtgcctgg
     9721 cacgttgttt gaagagctgg gatttaacta catcggcccg gtggacggtc acgatgtgct
     9781 ggggcttatc accacgctaa agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat
     9841 catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gacccgatca ctttccacgc
     9901 cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg gtttgccgag
     9961 ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca acaagctgat
    10021 ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac gtaaattccc
    10081 ggatcgttac ttcgacgtgg ctattgccga gcaacacgca gtgacctttg ccgccggtct
    10141 ggcgattggt ggatacaaac ccattgtcgc gatctactcc accttcctgc aacgcgccta
    10201 tgatcaggtg ctgcatgacg tggcaattca aaagctaccg gtcctgttcg ccattgaccg
    10261 cgcgggcatt gttggtgctg acggtcaaac tcaccagggc gcttttgatc tctcttacct
    10321 gcgctgcata ccagaaatgg tcattatgac cccgagcgat gaaaacgaat gtcgccagat
    10381 gctctatacc ggctatcact ataacgatgg cccgtccgcg gtgcgctacc cgcgcggcaa
    10441 cgcggttggc gtggaactga cgccgctgga aaaactacca attggcaaag gcattgtgaa
    10501 gcgtcgtggc gagaaactgg tgatccttaa ctttggtacg ctgatgccag aaacggccaa
    10561 agtcgccgaa tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac cacttgatga
    10621 agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag aagaaaacgc
    10681 cattatgggc ggcgcaggta gcggagtgaa cgaagtgctg atggcccatc gtaaaccagt
    10741 acccgtgctg aacattggcc tgccggactt ctttattccg caaggaactc aggaagaaat
    10801 gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg cctggctggc
    10861 ataatcccta ctccactcct gctatgctta agaaattatt catggcagga gtggcagcat
    10921 gcaatacaac cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc tcggctgtat
    10981 gacctttggc gagccagatc gcggtaatca cgcatggaca ctgccggaag agagcagccg
    11041 tcccataatt aaacgcgcgc tggaaggtgg cataaatttc tttgataccg ccaatagcta
    11101 ttccgatggt agcagcgaag agatcgttgg tcgcgcactg cgggatttcg cccgtcgtga
    11161 agacgtagtc gttgccacca aagtgttcca tcgcgttggt gatttaccgg aaggattatc
    11221 ccgtgcacaa attttgcgct ctatcaacga cagcctgcga cgtctcggca tggattatgt
    11281 cgatatcctg caaattcatc gctgggatta caacacgccg atcgaagaga cgctggaagc
    11341 cctgaacgac gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat ccatgcacgc
    11401 ttcgcagttt gctcaggcgc tggaactaca aaaacagcac tgctgggcgc agtttgtcag
    11461 tatgcaggat cactataatc tgatttatcg cgaagaagag cgcgagatgc tgcctctgtg
    11521 ttatcaggag ggcgtggcgg tgattccgtg gagcccgctg gcgcgggggc gactgacgcg
    11581 tccatgggga gaaactaccg cacgactggt gtctgatgag gtggggaaaa atctctataa
    11641 agaaagcgat gaaaatgacg cgcagatcgc agagcggtta acgggcgtca gtgaagaact
    11701 cggtgcaaca cgagcacaag ttgcgctggc ctggttgtta agtaaaccgg gcattgccgc
    11761 accgattatc ggaacttcgc gggaagaaca gcttgatgag ctgctgaacg cggtggatat
    11821 cactttgaag ccataacaga ttgccgagct ggaaacgccg tataaaccgc atgcggtcgt
    11881 aggatttaaa taagagtcca ggcctgatga gacgtgacaa gcgtcacatc aggcatcggt
    11941 gcacaactac gacagaatac ccagcggcca gtgatgaccg ataaagtaca ggatgcctgc
    12001 ggaaatcacc ccggcgacaa tatcgtcgat catgatcccc atgccgccat gtacattgcg
    12061 atcaaaccag cggatcggcc acggcttcca catatccaga atacggaaaa tcacaaaccc
    12121 ggcggcaacc cactgccagt cattggtcgg cagcgccatg agcgtgatcc acataccaat
    12181 aaattcgtcc cagacaatgc tgccgtgatc gtgcacgccc atatctttcg ccgtttgatg
    12241 acaaagatag acgccgatac agatccccaa catcaccacc agcgagtaga gctgccaggg
    12301 taaaaaggtc atcagatacc agaaagggat cgccgccagc gatcccatcg agccaggaac
    12361 gattgggctt aatccacttc cgaatccgac agcaagcaga tgccacggat tactcatctt
    12421 caggcgactt ttcgcgacat ctttatggcg tgacaaaatg gtcatatcct ttccagtcaa
    12481 acgtgacagg ttcgccgtca cgaataaaac aaagcccttc gatatcggcg gtcatttgcc
    12541 cgatacaggt aaacggtacg ccaaggtgtc cgagagcaac atccagcgcg ccacggttca
    12601 gttccggcac cgtgaaacac aactcgtaat cttcaccgcc agagagcgcc cagcgcagcg
    12661 cctgttccgg ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat gccaggtcaa
    12721 tacgtgcgcc gcagtcgctg gctttcacga tatgcccgag atcggaaatc agaccgtcag
    12781 agagatcgat agctgaattt gccagatcgc gcagtgcctg cccctgtaaa atacgcggcg
    12841 atggacggag atgacgtttg atcaagtagt cagcatcttt agcatcggca acctgcaaac
    12901 ggttttgcaa aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc acatagatcc
    12961 agtcacccgg tttcgcccca gagcgcgtta aggctcgtcc catcggaaca aagccgtgga
    13021 tacccaacgt cattgataat ggcccacgcg tggtatcgcc gccaatgagt tgcatatcgt
    13081 aataattgag aagatcaaac aaactgtcgc tgaaggactc aagccacgct tcgtctacgt
    13141 ccggtaaggt taatgccagc gtcagccagg ccggatcggc ccccatcgct gccagatcgc
    13201 ttaggttcac cgccagtgct ttataagcca gatcagcagg atcgatatca gggaggaaat
    13261 ggttgcccgc caccagcgta tcagtgctga tcgccagggt ctgtttctcg gggatattga
    13321 gaagagcgca atcgtcgccg atgcccagtt cgacatcaag acgagaactt cttacacggt
    13381 caaaataacg ggcaatcagg gagaactcgc cacatgccat acgttatgcc tcagcaggta
    13441 aaaaagaaaa ggccggcgat catggaacag tcttccgtga atctaccggc ctggatatta
    13501 ctttttgtta gggcgaatca caggtgctgc tttatcgagt acgccgttga cgaacttatg
    13561 gctgtcttct gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg ccactttgta
    13621 tggcacatca ctacgtttag acagttcgta cagcgcaatg cgcagtactg ctttttctac
    13681 ctggcccagt tcttccagca ggcgggacag gtagggcttc atcagtccgt cgaggtatgc
    13741 ggtgttagtc gccaccccgg ccagcagctc acggaagtac aggacgtcaa cgtcttttac
    13801 atcctgttca gccaggaact ggtattcaac atcagcgatg tcgttctggg acaactgcca
    13861 ggagtagagc gcctggacgg cacactcacg agcgcggcga cgagcagcag gtttcacgga
    13921 tttcccctta ctaatttcag gccttgatgg ctttcaatac attaatcatt tcaagcgcgg
    13981 tcagtgcagc ttctgcacct ttgttgccag ctttggtgcc agcacgttcg atcgcttgtt
    14041 caatgctttc agtggtcaga accccaaaag caaccggaat ttcgctgtcc tgggcaacat
    14101 gcgccaggcc gttgcttgca ccaccagcga catattcaaa gtgggcagtg ccaccacgaa
    14161 taaccgtacc cagcgcaatc accgcgtcgt atttaccggt tttagccagt gcacccgccg
    14221 ccagcggcag ctcataggca ccaggcaccc aaacaacggt aatgttttca tcttttacct
    14281 gaccgatacg tttcagtgcg tcaattgcac cttccagcag gctgtcattg ataaagttgt
    14341 tgaaacgcgc gatggtgatg gcgacgcgag cgtccggggt agcaacgtta gcttcaataa
    14401 tgttcatttt cttccttcgg gttcgagtat ggccccgcag gggggcggat tttagcataa
    14461 tatttcgtgc gctgcttccc tttcgagccg ggagatcatg cacccactaa atgcaggcaa
    14521 acatccgggc ctacatgacg tatctctttg aatttaaatt ggggggcgtc ggctaatttc
    14581 tcaagccctg gcagcgagca taatccacgg gcgtcgctgc ctaatagttt aggtgcgata
    14641 tagacaatca gctcatcgac taaacccgcc tgtagcaata cgccagcgag cgttggcccc
    14701 gcttccaccc agatgctgtt aatctgctgt ttacccagtt gcatcatcag tacaaccaga
    14761 tccagatgac ctttatgctc tggaatcagc aaggtacgca ccgtttccgg ccactcacga
    14821 gaatcttcct gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat gcgatgttcc
    14881 ggcgtcacgc gattttggct atcaatcaca atacgtactg gctggcggag attttgttgc
    14941 gggtagagcg cctgagtttg ttcatccagt tcagaccaac gcactgttaa ggctggatca
    15001 tccgccagca ccgtggcgct gctggttaaa atggcatgac tttgtgcgcg ctgtcgttgt
    15061 acgtcgcgcc gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc catcgccgtg
    15121 cggccatcaa gcgatgcgcc aagttttaac tgaatataag gaaagccggt gcgcatccgc
    15181 ttgagaaagc ctttattcaa ttgctcggct tcactcatca tcagcccgtg gctgacgtca
    15241 atgccagcct gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt aggatcttgc
    15301 atcgctgcga ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca cggtggcgta
    15361 cggccatgat ggctacaggg ttcgagtgtc acatacgccg ttgcgccttt ggctttttca
    15421 cccgccatac gcaacgcgtg tacttcggca tgtggttcac ccgctcgttg gtggtaacct
    15481 tcaccgacaa tttcgccatc tttgacaatg acgcacccga cattcgggtt ggggtgggtg
    15541 gtaaaacgtc ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc gtcctgcacg
    15601 gcttagtcct ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc gaaactgcgg
    15661 tagacagagg caaaacggat ataggcgact ttatcgagct ttttcaattg ctccatcacc
    15721 agattgccaa tcatcttgct cggtacttcg cgctcaccgg tggcgcgcag ctgcgattta
    15781 atatgattga ttgccatttc aacgtaatcg gaactcaccg gacgtttttc cagcgcccgc
    15841 agcattccgc tacgcaattt ctcttcatta aacggttcac gcacgtcgtt gctttttaca
    15901 acacgcggca taaccagctc cgccacttca aaggtggtga aacgttcatt acacaccaga
    15961 cactgccgac ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc aattacctta
    16021 gtgtccacgg cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt ggttaacgga
    16081 aatctatttt accctgaact gacgatacaa caaaggcgca acgcttttga gataaaggat
    16141 cgatgctttc gctgctttta ccctctacca ttaggctaat tgcgacgaga aagggacgac
    16201 ctatgaatac taacgttttt cgactgctcc tgctgggaag cctgttcagc cttagcgcct
    16261 gtgtgcagca aagtgaagtg cgacagatga aacacagcgt cagcacgctg aaccaggaga
    16321 tgacgcaact caatcaagaa acagtcaaaa tcactcagca aaacaggctg aatgcaaaat
    16381 ccagcagtgg ggtttacctt ctgcctggag cgaaaacacc ggcaagactg gaaagccaga
    16441 tcggtacttt acgtatgtcg ctggtgaata ttacgcctga tacagatggc accaccctta
    16501 cactacgtat tcagggtgag tcgaatgatc ccttgcccgc attcagcgga actgttgaat
    16561 atgggcaaat tcagggaaca atagacaact ttcaggaaat caatgtgcag aatcaattga
    16621 ttaatgcccc tgccagcgtc ctcgccccca gcgatgttga tattccgtta caattaaagg
    16681 ggatttctgt ggatcagtta ggctttgtac gtatccacga cattcaacct gttatgcagt
    16741 aaacgtattt cgggacgatt ttgtgcgtcc cgcaacatct tccccgtcat tttgttactc
    16801 tgcttacatc acctggattg atagtaaaag tttgcaacaa gggcgaaagt cagtacaatc
    16861 cccgcccgaa tgtgtgtaaa cgtgaacgca atcgattacg taaatgatag aactgtgaaa
    16921 cgaaacatat ttttgtgagc aatgattttt ataataggct cctctgtata cgaaatattt
    16981 agaaacgcaa tttgcgcctt tttcactccc gcaagggatt ttcaaacagt ggcatacata
    17041 tgaaaaaaac attactggca gccggtgcgg tactggcgct ctcttcgtct tttactgtca
    17101 acgcagctga aaacgacaaa ccgcagtatc tttccgactg gtggcaccag agcgttaacg
    17161 ttgtcggaag ctatcacacc cgtttcggac cgcagatccg caacgatacc taccttgagt
    17221 acgaagcatt cgctaaaaaa gactggttcg acttctatgg ttatgcggat gcgccggtat
    17281 tcttcggcgg taactccgat gctaaaggta tctggaacca cggttctccg ctgtttatgg
    17341 aaatcgaacc acgtttctcc atcgacaagc tgaccaatac tgaccttagc ttcggtccgt
    17401 tcaaagagtg gtacttcgcg aacaactaca tttacgacat gggtcgtaat aaagatggtc
    17461 gccagagcac ctggtacatg ggtctgggta ccgatatcga cactggcctg ccgatgagcc
    17521 tgtccatgaa cgtctatgcg aaataccagt ggcagaacta tggcgcagcg aacgaaaacg
    17581 agtgggacgg ttaccgtttc aaaattaaat actttgtgcc gattaccgat ctgtggggcg
    17641 gtcagctgag ctacatcggc ttcaccaact tcgactgggg ttccgattta ggggatgaca
    17701 gcggtaacgc aatcaacggt attaagaccc gtactaataa ctctatcgct tccagccata
    17761 ttctggctct gaactacgat cactggcact actctgtcgt agctcgttac tggcacgacg
    17821 gtggtcagtg gaacgacgat gcagaactga acttcggcaa cggcaacttc aacgttcgct
    17881 ctaccggctg gggtggttac ctggtagtag gttacaactt ctgattatga aaatgccggg
    17941 atttattccc ggcatttctg attgttaaca acgtgtgctt tgttcatgcc ggatgcggcg
    18001 taaacgcttt gtccggccca caaaagcacg taaattcaat atattgctga aataacgtag
    18061 gcctgataag cgtagcgcat caggcaattt tacgttttaa tcacttcttc ttgttcatca
    18121 tcgctttcag gtcagcgaat gggttgtact tcgcttcacc gacatctttc tgcgcatctt
    18181 cccctgcgat aacggtcgta ccatactgat ccgcttcggt atatttcgaa tgctcatgat
    18241 cgtggcaata gagacacaac aattcccagt tactgccatc ttccgggtta ttggtatggt
    18301 cgtgatcaat gtggtgaacg gtaagttcac gcaggttaga ataaacaaac tcgcgggaac
    18361 agcgaccgca gacccacgga tagattttta atgctttttc gcgatagccg ctttctaacc
    18421 gtgcatagtt ttttgggatg atagccatga aaagttacct ggatgaaatg tagggttacg
    18481 cgcagtttcc caagatgcgg ggattttcgc aatctccata catcaaaaaa aatcccgatc
    18541 ttctgaccgg gattttcaac atcaacggga acttgattaa ggcagaattg acggctgatc
    18601 cgccccttct ttttccactt tctgctgcaa catgtgttcg cgcttcatac ccagtttcag
    18661 agccaacgca gatgctacgt agatggaaga tgcagtgccg atggaaacac cgataagcat
    18721 ggtcagcgag aagccttcca gtaccggacc accgaagaga tacagcatca ggataaccat
    18781 caaggtagta ccggatgtga tcaaggtacg gtgcagcgtc tgggtcaagg acacgttaaa
    18841 gatttcgtaa ggcgtaccgc gacggatctt gcggaagttt tcacgaattc ggtccgatac
    18901 cacgatactg tcgttaagcg agtaaccgat aaccgacatc aacgatgcca caatggtcag
    18961 gtcaatctcg atatggaata acgacaaaat acccagcgta atgataacgt cgtgcgtcag
    19021 cgcaataacc acccctgccg ccagtcgcca ctcaaagcgg aaacctacgt acacgaggat
    19081 agacagcagc gctgccatca acgccatcgc accggtttgc gcaaggtcag cccccacgct
    19141 cggaccgacg aactcaatac gcttcactgc tgcattctga ttggtggatt cgttaatcac
    19201 cttcagaact tggctgccca gcacctgacc gccggtttcg ccttcagcag gcggcatacg
    19261 gaccatgatg tcgtggctgc taccaaagtt ttgcagcatt ggctcttcaa aaccggcttt
    19321 ttgcaatgca tcacgcatta cgtcaatttc agccggtttt tcgagcgtaa tttcgataac
    19381 cgtaccaccg gtgaaatcca gcccccagtt aaagccgcgc acgcccataa taacgatagc
    19441 agcgattaac agcagaccag agatgccgaa agcccagtag tcccagcgca taaagtcata
    19501 gactttacgg ccgtggttta gttgttcaac agtatattcc tgtgccacat cgcactcctc
    19561 agattgacag cttcttgacg cgcttgccgc catatagcag gtttacgatg gcacgcgtac
    19621 cgacaatcgc ggtaaacatc gacgtcgcca caccgatacc ggtagtaatc gcgaaccctt
    19681 taattgcccc ggtacccact gcgtacagga tgatgacttt aatcagcgtg gtgatgttcg
    19741 catcgaagat agaactgaat gcgccacgat aaccttcatc aattgcctgt tgaacagtac
    19801 gtccgttgct caactcttct ttaatacgtt cgttgatcag tacgttcgca tcgaccgcta
    19861 ccgcaagggt taagacgata cccgcgatac ctggcatact cagtgttgcg cctggcaaca
    19921 gcgacataat accgacgatc aagatcaagt tggcaatcag agcactggtc gcaatcagac
    19981 caaacttctt atagaagatg atcatgaaca gaatagaaac cagcagaccg gcaaggcaag
    20041 cctccagccc ctgctcaatg ttctgcatac ccagggtcgg gccaattgta cgttcttcaa
    20101 caatctgaat cggcgcgatc aacgcaccgg cacgcagcag cagtgacagc tgacgggctt
    20161 cgttcgggtt gttgatgccg gtgatacgga agctgttacc cagacgagac tggatgttgg
    20221 cgatgttaat cacctcttcc tgtttcacca gaaccgcacg accatttgca tctttcttac
    20281 cgctgtcttt gtactccaca aacagggttg ccatcggttt gccgatgttg tccttggtga
    20341 agttagacat aatgttgcca ccagcgctat cgagcgagat gttaacctgc ggctggttgt
    20401 attcgtcctg gctggaagtg gagtcggtga tatggtcacc agtcagaatt acgcgtttgt
    20461 acagcacaac cggctgacct tcgcgggtct gtttcacttc agagtcgccc ggtacgcgac
    20521 cggatgccgc agcggcctga tcaacattgg tgtttaccag acggaattcc agcgttgccg
    20581 tcgcgcccag aatctctttc gcacgcgcag tgtcctgaat accgggcagt tcaacaacga
    20641 tacggtcagc accctgacgc tgaaccaccg gctccgccac gccaagttgg tttacacggt
    20701 tacgcaggat attaatgttc tgctgcaccg catattcacg cgcttcactc agacgagcat
    20761 cgctcattac cgcacgcagc tggttgctgc cctggctgct aatcaccagg tccggatggc
    20821 gcttgctcag atacgcaatg gcttcatcac gagctttagc atcgcggaaa gtgatgctca
    20881 ggccgtagtt gttttcttta cgaacagtgg tatacgggat gcccttttcg cgcaggtcac
    20941 tgcgtaggct atcgatattt tgttcctgga gtttgccaag cgcggtgtcc atatccactt
    21001 ccatcaggaa gtgaacgccg ccacgcaggt caaggccgag cttcatcggc tcagcatgaa
    21061 tagctgccag ccagcgcggc gttgccgggg caaggttaag cgccacgacg tatttgtcac
    21121 ccataacgcc cattaatgct tcacgagcgc gcaactgggt gtcagtggag tcgaagcgcg
    21181 caagaatagc gccctcttcc agtgccacag acttagcagt tattttttct tcttgtaacg
    21241 ttttctggac ctggatcagc gtttgctcac tggcggcgac tccgcgcgca ccagtgatct
    21301 gaacagccgg atcctcacca aacaggttgg gaagcgcata cagcagaccg atgacaatca
    21361 ccacgatcag catgacgtac ttccacaaag gataacggtt taacacggca attcccttag
    21421 ggaaaaattt taattacagc gccttcatgg tgcctttcgg caggacggca gctacgaagt
    21481 cacgtttaat aactacttca gtggtgtcat tcagcgcgat agcaatgtag ccgttttccg
    21541 ctactttggt tacgcgacca accaggccac cgttcgtcag aacttcatca cctttggcaa
    21601 tggagtccat cagctttttg tgttctttgg tgcgcttctg ctgtggacgc aggatcatga
    21661 aatagaaaat cagaccgaat accaccagca tcaaaatcaa agacatcggg ctaccttgcg
    21721 ccggtgcacc cgttgccgct accgcatcag aaataaaaaa gctcattaaa tttccctcat
    21781 tattaatatt aatcaacgtt caaaggtggt acttctcgcc cctgacgctg gtaaaaatca
    21841 gttacgaagc tctctaattt accctcttca atagccttgc gtaaacccgc catcaaacgc
    21901 tggtagtaac gaaggttatg aatggtgttg agtcgcgcgc ctaatatttc gttgcaacgg
    21961 tcgagatgat gcaagtaagc gcgtgaataa ttgcgacagg tgtagcaatc acactcagga
    22021 tcgagtgggc cagtatcgct cttatatttc gcattgcgga ttttcaccac gccatcggtc
    22081 acgaacaaat gaccatttcg ggcgttgcgg gttggcatta cgcagtcaaa catatcgata
    22141 ccgcgacgta cgccttcaac caggtcttcc ggtttaccaa cgcccatcag gtaacgcggt
    22201 ttgtctgccg gaatttgcgg acacacatgc tccagaatac ggtgcatatc tgctttcggc
    22261 tcacccacag ccagaccgcc gacagcgtag ccatcaaagc cgatatctac cagaccttta
    22321 acagatatat cacgtaaatc ttcgtaaata ctgccctgaa ttataccaaa taacgcattt
    22381 ttgttaccaa gactgtcaaa acgctcacgg ctacgcttcg cccaacgcag agacatctcc
    22441 atagagcgtt ttgcgtaatc ccagtcagca ggatacggcg tacactcatc aaagatcatg
    22501 acgatatccg aaccaagatc gtactgaatc tccatcgact tttccgggtc gaggaaaatc
    22561 gggtcgccgt tgatcgggtt acggaagtga acgccctgtt cggtgatttt acgaatatca
    22621 ccaaggctga agacctggaa gccgccggag tcggtaagaa tcggtccttt ccactgcata
    22681 aaatcgtgca gatcgccgtg cagtttcatg atttcctggc ccgggcgcag ccacaggtgg
    22741 aaggtgttgc cgaggataat ttgcgcgcca gtggcttcaa cttcttccgg cgtcatccct
    22801 tttacggtgc cgtaggtgcc aacaggcata aaacaaggcg tttccactac gccacgatca
    22861 aagaccaggc ggccacggcg tgcgcgaccg tcggtggtgt ccagttcaaa tttcattttt
    22921 tctccactac gtcagaaaaa cagtccaacg tttaaaccag cgccgcggaa ttactccccg
    22981 acgcgctcat taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa
    23041 aagcgatatt tctcttctac cgccgcttta taggcattca tggtgtgttg ataaccggca
    23101 aaggccgaaa ccagcataat cagcgtcgac tctggcaagt ggaagttcgt caccagcgca
    23161 tcgaccactt tgtactggaa gcccggatag ataaagattt gggtatcgtc aaagaacggt
    23221 tcgataagat cgttttttgc cgcctgtgcc gcgctttcca gcgaacgtac tgaagtggtg
    23281 ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc taccacatcc
    23341 tgcggtactt cagcgtattc cgagtgcatg atgtgatctt caatggtgtc gacgcgcacc
    23401 ggctggaagg tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac gcctttggcg
    23461 cgtaattttt ccagcaaagg ctcgtcaaaa tgcagaccag cggtcggggc tgcaaccgcg
    23521 cccggttttt cgctataaac ggtttgataa agttcgcggt cagcgtcttc gtccggacga
    23581 tcgatatacg gcggcagcgg catatggccg atgctgttga ggatatccag taccgagcgt
    23641 tcatcattaa attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttgata
    23701 ctttcgtcat cgcccagcaa cagttctgcg ccaggttttg gcgctttcga ggcgcgaata
    23761 tgcgcaagaa tgcgtttgtc gtcgagcatc cgctcaacca gcacttcaat cttgccgccg
    23821 ctggctttac gcccaaacag acgcgccggg atcacgcggg tattattaaa aaccagaaga
    23881 tcgccggggt tgagcttatc gagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc
    23941 ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag gcatgggata gtgggcaatc
    24001 agggattcgg gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac tcagactaaa
    24061 ataagagtcg ggtagtctag tgccggggcg ctctccctgc aacctttacc cccctttgga
    24121 tagaatataa tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc
    24181 gctttctggc aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc
    24241 cgacgtcgtg gctggcattc atatgcatcg acgtattgac tgacaatctg ccggaagtcc
    24301 gcgaagcaca ggagtggttt cgtagtgaaa cgcgccgcgt tgcgcctatt acgctggatg
    24361 tcatgtggga tcactttctt tcccgccact ggtcgcagct gtcgccggac tttccgctac
    24421 aggaatttgt ctgttatgcc cgcgagcaag tgatgacgat tttgccggac tcaccgccac
    24481 gttttatcaa tctgaacaat tacttgtggt cagagcagtg gctggtgcgc tatcgcgata
    24541 tggatttcat ccagaacgtg ttaaacggca tggcaagccg ccgcccacgt ctggatgccc
    24601 tgcgtgactc ctggtacgat ttagacgctc attatgacgc cctcgaaacc cgcttctggc
    24661 agttttatcc gcggatgatg gcgcaggcgt cacacaaggc gttataaaac gttagttcat
    24721 ccataccgta gccgaaatgg caggcaaagc cagaatcccg tcagtcagtt gcccatgccc
    24781 ttctttgcat tgccattgca cggcattgag aaacggtgac gcgggtagca ccacttcact
    24841 ggcctcgcca cggttgattg ccaccagtac acgttgctga ttcagcacgc ggacaaatac
    24901 cactacgtta tcttccgcat acagcacctg acagccgcca cgacgcagcg cctgactttt
    24961 cttacgcagc acaatcattc gccggtacag cgcgaataac gccgtatcct gcttttccac
    25021 ctgccagggg aacggtttac ggcaaaacgg atcgttttta ccatccagtc ccacttcatc
    25081 accgtaataa atgcacggta caccaggcca ggtgaacagc cagaccaccg ccagcggcag
    25141 gcgcgcaata tcccgaccga gcagcgtttt aaatcgcgca gtatcgtggc tatcgagctg
    25201 attaaacata cgtaattgtt gttgatgaga aagccctgcg cggtaattat ccatccaggc
    25261 catacaggtt tgggcgtcaa tttgctgcgg atcataagag atgtcggtat tggcaagaaa
    25321 tccccacaac gggaatgtga agccacgata gttcatggca gcatcttcca catcggcctg
    25381 taaccattgc cgtgcatcgc caaaatgttc gccgacaata tacgcttccg gctgggtttc
    25441 tttcgccgct tcggtgatcc cggcaacgtg ctgcatatta ttgcgcgccc cacccgcctc
    25501 ccccagcata tgcaccacat ccagccgcca gccgtccata ttccacggcg ctttcagcca
    25561 gtggcggaca atactgtctt ccccgcgata aatttcattc accagacttt ccgactgata
    25621 atccagcttc gtcaagctgg catagccaag ccagtcgagc gccgtgccat catcactaaa
    25681 cgagtaccag tcgcgccagg gcgattcaga gttgtgacaa gctccccccg tgccaagatt
    25741 gtgcctgtca aaccaggcat gggaatcgcc actgtggtta aacacgccgt ccagcaccag
    25801 ccgcattccc agctgctgcg tattgtgtcg caaacgcagc aacgccccat caccgccaaa
    25861 ctgcggatcg acatggcgat aatcctcggt atcgtattta tgtacgctgg gagctttaaa
    25921 caccggattg agatacagcg ctgtcacgcc aagctttttc agatacggca gtttttcgct
    25981 tatcccgtcc agatcgccgc catagaacgt tgatccgccc gcctgcgccg tgaccggttc
    26041 atcccagtca cgcaagatga tctcttgtcc ggctgcatga tggtaataga catgatcctg
    26101 ttcagcttca cgaggaagac tacgcgcaaa acgatcaggg aagatctgat aaaaaatctg
    26161 atccgcagcc cattgtgggc cgatatcagg tacatcgacg gcaaactgct ccagtcgtgc
    26221 cggcggcatt cggctgaagc cctgcggtgt aaaccaacgc tggcgatcgt gccacagcag
    26281 tttgaaactg taacgccgcc ggggttgtcc gctggagaga tcaatcgccg cacgccatgc
    26341 ggtgacgcct ggctgcggct gactgcgctg cttatgcatt ggtactgaca tttcttcgtt
    26401 atcgtgttct gtacgcagca taatgcgctg cggtgggtct tcgcccgtca gccacagtgt
    26461 aatgagcagt tgatctttgc tttgtttaac aaatgggggc accggcaggt gccatgcatt
    26521 taacatcata attcccctgt gatgaaaatc tgatcacctt gccatagcct aatgcaggat
    26581 cactcatcat ctgaaggata tttgggggag gaggacgggg gcggataact ttcggaatag
    26641 ccggatcagg atttcctcct gaaccggctt acagaacgca agtattattg gttttcagcc
    26701 agctgacgat cgtggcggcg tttaaacatc cagccaatca acagcacaac aatccacgcg
    26761 aaaccgacat acagcgagat acgcgtatcc gggtgataac caatcaaccc gataataaag
    26821 agcaggaaaa tcagcccgcc gatggtcgtt gctaccccac ccggcacttt aaatttcagc
    26881 gccttaactt cttctggcgg caaacggcga cggaaggcga tttgcgacag caggatcata
    26941 atccacaccc ataccgtggc gaaggttgcc agcgaagcga tcaccaggaa gacgttttcc
    27001 ggcatgatgt agttcagata caccgcaaac agcagcgcgg tagtcatcac cagcaccgta
    27061 acccacggaa taccgcgacg cgacgttttg ctgaaaattt tcggtgcgct gccctgctct
    27121 gccataccgt ggagcatacg gcctacgcca aatacgtcac tgttaattgc cgacagcgaa
    27181 gcggtcagca caacaaagtt aagaatgctg gcggcaaagg taatgcccat atgctggaac
    27241 gtcagtacga acgggctacc ggcagtgcca acctgattcc acgggtagat agacataatg
    27301 acgaacagcg taccgacgta gaacaccaga atacgcatcg gcacggagtt aatcgcacgc
    27361 ggaatcgatt tctcaggatc tttcgcttca ccggcggtaa tcccgataat ttcgatccca
    27421 ccgtaagcaa acatcaccat ttgcaacgac attaccatgc caagccagcc gttactgaag
    27481 aagccgccgt tgctccacag gttatgaata ccggtcggtt gcccgccgtt gccaatcccc
    27541 cagatgatga tgccgaaacc ggcgacaatc atgatgatga tggtggcgac tttaaagaac
    27601 gagaaccaga attccagctc accgaagacc ttcacgctca tcaggtttac ggcgcaaatg
    27661 atcagcacca cgctcagtac ccaaatccag tgcggcaccg tcgggaacaa gacacccata
    27721 tagataccaa aagcggtcac atcggcgatg gcgacaataa ggatttcaaa acagtaggtc
    27781 catccggtaa tgtaacctgc cagcgggcca aggttttcct gcgcataacg cgagaaagag
    27841 ctggcggccg ggttatgtac cgacatttcc cccagcgcac gcataatgat atacgccgcg
    27901 ataccaccga taatataggc caacaacacg ctcggaccgg ccattttgat ggcgtctgcc
    27961 gaaccgtaaa acagcccggt gccaattgct gaacccagtg ccataaagcg tatgtggcgg
    28021 gtacttagcc cacgctttag cttattctta ctttccatca attcctaacc catcaacaca
    28081 ataaaaaacc acggggcatc gcagccccgt ggttaaaaca aatgttcagt gatttagtga
    28141 gcgctggagg tcacctgacg acctgccgcg cgatcccaga taatggccag aaccaccatc
    28201 accactgttg gcattaacca cgccagacct tgttcggcca gcggtaaacg ctgcgcccag
    28261 gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt cgagaatacc aaaaagcagg
    28321 ctgataaaca tcggcggagc aatcacgcgg gacgaattat gccaccatga gcgtgtaaaa
    28381 cttaatacaa ccagtgcgat acacggcgga taaatagcgg tcagcaccgg tacggagatc
    28441 tggatcagct ggcttaagcc gaggttagac accaccatcg agaagccgcc gaggataaac
    28501 accagcgtac gataagagag cggtacgtac tgggcaaaga attctgcaca agcacaggtc
    28561 aggccaaccg ccgtgaccag acaggcgatg aagattaacg ccgccagcag gaagctaccg
    28621 ccgccgccaa aggtgtgctg aacgtaagca tgcagaatag cagcgccgtt tgcagactga
    28681 tcgaccagcg acgcgctgtc tgaacccaga cagaacagcg ccaggtacag caaagtcaga
    28741 ccaacacccg ccatcaggcc agcccagacg gtataacggg tcagcagacg cgcttcggta
    28801 acgccacgag aacgcgccgc gttaacaata acgataccaa acaccattgc gcccagcgta
    28861 tccatggtca gatagccgtt aacgaagccg ttagaaaatg cagcgttttg ataagcctca
    28921 gtcgccgtgc tgatagaacc cgccggccag acaatcgcga caacagacag gatgaccagc
    28981 gcgataattt tcagcggcgc aaggaagttg cccacggtat ccagcagctt gcccggatag
    29041 agcgaaacca gaataacgat agcgaaatag accaggctgt agataaacag cggcagcgcg
    29101 gaatcacccg tcagcggcgc aatccccact tcaaaggaaa cggtagctgt acgcggcgta
    29161 gcgaaaagcg gccccaccgc caggtaacaa accgttgcca gcagtacgcc agcgacttta
    29221 ccgattggcg tgctgaggct gtcaacaccg ccgccaactt ttgccagcgc cactaccgtt
    29281 aataccggca ggccaacggc agtaatgagg aagccgaatg ccgcagtcca gacgtgttcg
    29341 cctgcctgta agccgaccat tggagggaaa ataatgttac ctgcgccgac gaacaacgca
    29401 aatgtcataa agcccagagc gatgatatcg cgcgatctta attgatgggt cataaatcat
    29461 actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg ccaatccctg gggatgatac
    29521 agcggtatat ttaaacaatt ttagcgggaa atactcccaa ccaggcgcgg cgaagaatag
    29581 tttttcgatt taccgcgtta atgcagtcag tatgacagca cctgaagcgc aatttaaacg
    29641 cttataacgt tttaaagcaa ggagggatct aaaaaccaga ggtatatagc gataagaaat
    29701 caccaaccag tataaaacac tatttccgtg aaaaagacat ggctaatcat gcgaacaaaa
    29761 aatcaccaac cgttgtaaaa tctaacgccc gtattttgac tgcaaagaaa ataagccagc
    29821 atcggctggc ttatggcaat aaaagatgac aaaggcggat taatcgctgt ttttggcaat
    29881 taaacgttcc gggataacaa aactgaaacg tgttcctttt cctactgtac tctcaatatt
    29941 caggcgactt tcgtgatgat tcaccgcatg tttcacgatc gctaacccta atccgctacc
    30001 gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa cgctcggtca ggcgcggaat
    30061 atgctccggt gcaatgcccg gtccgttatc ttcaacgcta aattcggcac cgtgcggcac
    30121 tcgctgccag cgtacggtga tatgcgtgcc ttccggcgta tgattcacgg cgttatagac
    30181 caggttcgaa atcgcactgc gtagctgatc ttcgttgcca gacaccttga ggccgttatc
    30241 tatctcaaag gtaaatgtct gttttttctg actcagagtc tgagcctcgc gctcaacaac
    30301 gcgcagcatc atcggcacat caaccttttc attgagcaat tgcgtcggcg cggcttcaat
    30361 tttcgacagc gttagcaact gcttcaccag cccttccatc cgctgggtct gctcgcgcat
    30421 ggtgtgcaac gctttttcgc gtaccgcgcc ttccagcggc tgctcatcca tcatctccag
    30481 gtaaccctgt aacacggtca atggcgtacg taactcatgg ctcacgttgg caaaaaagtt
    30541 acgccgcgcc ccttccagtt gatgcatttg cgtgacatca cgcgccacca tcagcaactg
    30601 tttgtgggta taaggcatga cgcgaatttc cagatgccgc ccggtgttga gcaccagatt
    30661 gagcgggcga gaaaaatcac gcgttttcag atattgcgta aactccgggt aacgcagtag
    30721 gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa ccaagaattt gttgcgccag
    30781 accgttacac cagaaaatac cgccctcttc cgtggtcagc accaccgcgt cgggcagcga
    30841 ctccgcgccg ctacgaaagc gtttaatcag attgcccagt tcacggcggc gttttttatt
    30901 tcgcagctgc atctggtgta agccgtatag tagcggttcc cagctaccac gccccggtgg
    30961 cggggtcata ctgcgatcca cccacagcca ccatgaaagg cgcaataaat tccagaaatg
    31021 ccagataagc agtcctgtta ccgatgccag caaaaaccag ggcaggtaac caaaaaatgc
    31081 acccaggatg aaagccggga ggcagcaaag tagcagctcc agcaccagcc ttttccacga
    31141 cagccgttcc agcacgtaag atactccagt taagaaatca taagccctgc tctgcgtccg
    31201 atgagcaagg cgttaaaagc gggttgaaaa acgatatcct gtaccgcgca cggtctgcac
    31261 catgcggtca tgcccgccgg gctccagtgc tttacgcagg cgacgaatgt ggacatcgac
    31321 cgtgcggtct tccacataca cgttagttcc ccagacgtgg tttaacagct gctcgcggct
    31381 gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt ttaaattctg tcggccccat
    31441 ctccagcggc tcttcgcccg ccatcactcg gtgagatgtc gggttgagac ttaatccctg
    31501 catcttaatc acctcttcca ccgccattgg cgaaatacgg cgcattaccg ctttgattcg
    31561 cgccaccagc tccttcggcg aaaatggctt ggtgatatag tcatccgcgc cggtttcaag
    31621 gccgcgcacg cgatcttctt cttcccctct ggcggtcaac atcaccactg gaatatcccg
    31681 ggtcatcgac tcgcgcttga ggtgtttgat gaactggata ccggagccgc caggtaacat
    31741 ccagtcaagg agaattaaat ccggccaggg ttcattcagt tgattcacag cactgtcata
    31801 atcttccgct tcgaccggct gaaagccatt ttgttcgagc acgaagcaga ccatttcgcg
    31861 aattggagct tcatcttcta cgaccagaat acgtctcgcc atgatttgcc ctgttgtaat
    31921 aaataggttg cgatcattaa tgcgacgtca ttatgcgtca gatttatgac agatttatga
    31981 aaagctcgtc gcacatatct tcaggttatt gatttccgtg gcgcagaaaa aagcaaatgg
    32041 cacatctgtt tgggtataat cgcgaccatg ctttttcgcc agggaaccgt tatgcgcatc
    32101 cttcacacct cagactggca tctcggccag aacttctaca gtaaaagccg cgaagctgaa
    32161 catcaggctt ttcttgactg gctgctggag acagcacaaa cccatcaggt ggatgcgatt
    32221 attgttgctg gtgatgtttt cgataccggc tcgccgccca gttacgcccg cacgttatac
    32281 aaccgttttg ttgtcaattt acagcaaact ggctgtcatc tggtggtact ggcaggaaac
    32341 catgactcgg tcgccacgct gaatgaatcg cgcgatatca tggcgttcct caatacaacc
    32401 gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc gtcgcgacgg gacgccaggc
    32461 gcagtgctgt gccccattcc gtttttacgc ccgcgtgaca ttatttccag ccaggcgggg
    32521 cttaacggta ttgaaaaaca gcagcattta ctggcagcga ttaccgatta ttaccaacaa
    32581 cactatgccg atgcctgcaa actgcgcggc gatcagcctc tgcccatcat cgccacggga
    32641 catttaacga ccgtgggttc cagtaaaagt gacgccgtgc gtgacattta tattggcacg
    32701 ctggacgcgt ttccggcaca aaactttcca ccagccgact acatcgcgct cgggcatatt
    32761 caccgcgcac agattattgg cggcatggaa catgttcgct attgcggttc ccccattcca
    32821 ctgagttttg atgaatgcgg taagagtaaa tatgtccatc tggtgacatt ttcaaacggc
    32881 aaattagaga gcgtggaaaa cctgaacgta ccggtaactc aacccatggc ggtgctgaaa
    32941 ggcgatctgg cgtcgattac cgcacagctg gaacagtggc gcgatgtatc gcaggagcca
    33001 cctgtctggc tggatatcga aatcactact gatgagtatc tgcatgatat tcagcgcaaa
    33061 atccaggcat taaccgaatc attgcctgtc gaagtattgc tggtacgtcg gagtcgtgaa
    33121 cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc tcagcgaact cagcgtcgaa
    33181 gaggtgttca atcgccgtct ggcactggaa gacctggatg aatcgcagca gcaacgtctg
    33241 cagcatcttt tcaccacgac gttgcgtacc ctcgccggag aacacgaagc atgaaaattc
    33301 tcagcctgcg cctgaaaaac ctgaactcat taaaaggcga atggaagatt gatttcaccc
    33361 gcgagccgtt cgccagcaac gggctgtttg ccattaccgg cccaaccggt gcggggaaaa
    33421 ccaccctgct ggacgccatt tgtctggcgc tgtatcacga aactccgcgt ctctctaacg
    33481 tttcacaatc gcaaaatgat ctcatgaccc gcgataccgc tgaatgtctg gcggaggtgg
    33541 agtttgaagt gaaaggtgaa gcgtatcgcg cgttctggag ccagaatcgg gcgcgtaacc
    33601 agccggacgg taatttgcag gtgcctcgcg tagagctggc gcgctgcgcc gacggcaaaa
    33661 ttctcgccga caaagtgaaa gataagctgg aactgacagc gacgttaacc gggctggatt
    33721 acgggcgctt cacccgttcg atgctgcttt cgcaggggca atttgctgcc ttcctgaatg
    33781 ccaaacccaa agaacgcgcg gaattgctcg aggagttaac cggcactgaa atctacgggc
    33841 aaatctcggc gatggttttt gagcagcaca aatcggcccg cactgagctg gagaagctgc
    33901 aagcgcaggc cagcggcgtc acgttgctca cgccggaaca agtgcaatcg ctgacagcga
    33961 gtttgcaggt acttactgac gaagaaaaac agttaattac cgcgcagcag caagaacaac
    34021 aatcgctaaa ctggttaacg cgtcaggacg aattgcagca agaagccagc cgccgtcaac
    34081 aggccttgca acaggcgtta gccgaagaag aaaaagcgca acctcaactg gcggcgctta
    34141 gtctggcaca accggcacga aatcttcgtc cacactggga acgcatcgca gaacacagcg
    34201 cggcgctggc gcatattcgc cagcagattg aagaagtaaa tactcgctta cagagcacaa
    34261 tggcgcttcg cgcgagcatt cgccaccacg cggcgaagca gtcagcagaa ttacagcagc
    34321 agcaacaaag cctgaatacc tggttacagg aacacgaccg cttccgtcag tggaacaacg
    34381 aactggcggg ttggcgtgcg cagttctccc aacaaaccag cgatcgcgag catctgcggc
    34441 aatggcagca acagttaacc catgctgagc aaaaacttaa tgcgcttgcg gcgatcacgt
    34501 tgacgttaac cgccgatgaa gttgctaccg ccctggcgca acatgccgag caacgcccac
    34561 tgcgtcagcg cctggtcgcg ctgcatgggc agattgttcc ccaacaaaaa cgtctggcgc
    34621 agttaatggt cactatccag aatgtcactc tggaacagac gcaacgtaat gtcgcactta
    34681 acgaaatgcg ccagcgttat aaagaaaaga cgcagcaact tgccgatgtg aaaaccattt
    34741 gcgagcagga agcgcgcatc aaaacgctgg aagcccagcg tgcacagtta caggcgggtc
    34801 agccttgtcc actttgtggt tccaccagcc acccggcggt cgaggcgtat caggcgctgg
    34861 agcctggtgt taatcagtct cgattactgg cgctggaaaa cgaagttaaa aagctcggcg
    34921 aagaaggtgc ggcgctgcgc gggcaactgg atgcattaac gaagcagctt cagcgcgatg
    34981 aaaacgaagc gcaaagcctc cgacaagatg agcaagcact tactcaacaa tggcaagccg
    35041 tcacggccag cctcaatatc accttgcagc cacaggacga tattcaaccg tggctggatg
    35101 cacaagatga gcacgaacgc cagctgcggt tactcagcca acgacatgaa ttacaagggc
    35161 agattgccgc gcataatcag caaattatcc agtatcaaca gcaaattgaa caacgtcagc
    35221 agcagctctt aacggcattg gcgggttatg cactgacatt gccacaggaa gatgaagaag
    35281 agagctggtt ggcgacacgt cagcaagaag cgcagagctg gcagcaacgc cagaacgaat
    35341 taaccgcgct gcaaaaccgt attcagcagc tgacaccgat tctggaaacg ttgccgcaaa
    35401 gtgatgatct cccgcacagc gaagaaactg tggcgctgga taactggcgg caggttcatg
    35461 aacaatgtct cgcattacac agccagcagc agacgttaca gcaacaggat gttctggcgg
    35521 cgcaaagtct gcaaaaagcc caagcgcagt ttgacaccgc gctacaggcc agcgtctttg
    35581 acgatcagca ggcgttcctt gcggcgctaa tggatgaaca aacactaacg cagctggaac
    35641 agctcaagca gaatctggaa aaccagcgcc gtcaggcgca aacgctggtc actcagacag
    35701 cagaaacgct ggcacagcat caacaacacc gacctgacgg gttggctctc actgtgacgg
    35761 tggagcagat tcagcaagag ttagcgcaaa ctcaccaaaa gttgcgtgaa aacaccacga
    35821 gtcaaggcga gattcgccag cagctgaagc aggatgcaga taaccgtcag caacaacaaa
    35881 ccttaatgca gcaaattgct caaatgacgc agcaggttga ggactgggga tatctgaatt
    35941 cgctaatagg ttccaaagag ggcgataaat tccgcaagtt tgcccagggg ctgacgctgg
    36001 ataatttagt ccatctcgct aatcagcaac ttacccggct gcacgtgcgc tatctgttac
    36061 agcgcaaagc cagcgaggcg ctggaagtcg aggttgttga tacctggcag gcagatgcgg
    36121 tacgcgatac ccgtaccctt tccggcggcg aaagtttcct cgttagtctg gcgctggcgc
    36181 tggcgctttc ggatctggtc agccataaaa cacgtattga ctcgctgttc cttgatgaag
    36241 gttttggcac gctggatagc gaaacgctgg ataccgccct tgatgcgctg gatgccctga
    36301 acgccagtgg caaaaccatc ggtgtgatta gccacgtaga agcgatgaaa gagcgtattc
    36361 cggtgcagat caaagtgaaa aagatcaacg gcctgggcta cagcaaactg gaaagtacgt
    36421 ttgcagtgaa ataactattc agcaggataa tgaatacaga ggggtgaatt atctcttggc
    36481 cttgctggtc gttatcctgc aagctatcac tttattggct acggtgattg gtagccgttc
    36541 tggtggttgt gatggtggta tgaaaaaagt cattttatct ttggctctgg gcacgtttgg
    36601 tttggggatg gccgaatttg gcattatgag cgtgctcacg gagctggcgc ataacgtagg
    36661 aatttcgatt cctgctgccg ggcatatgat ctcgtattat gcactggtgg tggtggtcgg
    36721 tgcgccaatc atcgcactct tttccagccg ctactcactc aaacatatct tgttgtttct
    36781 ggtggcgttg tgcgtcattg gcaacgccat gttcacgctc tcttcgtctt acctgatgct
    36841 cgccattggt cggctggtat ccggctttcc gcatggcgca ttttttggcg tcggagcgat
    36901 cgtgttatca aaaattatca aacccggaaa agtcaccgcc gccgtggcgg ggatggtttc
    36961 cgggatgaca gtcgccaatt tgctgggcat tccgctggga acgtatttaa gtcaggaatg
    37021 ctggcgttac acctttttat tgatcgctgt ttttaatatt gcggtgatgg catcggtcta
    37081 tttttgggtg ccagatattc gcgacgaggc gaaaggaaat ctgcgcgaac aatttcactt
    37141 tttgcgcagc ccggccccgt ggttaatttt cgccgccacg atgtttggca acgcaggtgt
    37201 gtttgcctgg ttcagctacg taaagccata catgatgttt atttccggtt tttcggaaac
    37261 ggcgatgacc tttattatga tgttagttgg gctagggatg gtgctgggaa atatgctaag
    37321 tggcaggatt tcaggacgtt attcaccact gcgcattgca gcagtgactg actttatcat
    37381 tgtactggca ctgctgatgc tctttttctg cggcggcatg aaaacaacgt cgcttatttt
    37441 tgcttttatt tgttgcgcgg gattatttgc cctttcagca cctctgcaaa tattgttact
    37501 gcaaaatgcc aaaggaggag agttattagg tgccgcaggt gggcaaatag cgtttaacct
    37561 cggtagcgcc gtcggcgcat attgcggagg tatgatgctg acgctggggc tggcatataa
    37621 ttacgtggcg ctgcctgccg ccctgctttc gtttgctgcg atgtcatcgt tgctgctgta
    37681 tggtcgctat aagcgccagc aagcggcgga tagtccggtg ctggcgaaac cactggggta
    37741 gattactgtc acggtgcgcg ttgcttattg ccggatgcgg cgtgaacgcc ttatccgccc
    37801 tacgcggttc tggcacattt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat
    37861 gatgcgccaa ttgcctacgt tttttactct tgtggccata accacgcagc gccgcgtacg
    37921 ccgctggaat caccgtgctt cgccttactc accggcgttt cacattcgcc gccgaagaca
    37981 aattgtttaa tcaactgccc aaccgtttga tataaacggt ctacattgct catcccgccc
    38041 cccaggacaa tcacatccgg atcgagaata ttcacgacat gtgccagcga ttttgccagc
    38101 cgcagctcgt agcgacgcaa tgccagttcc gctaccggat cgctttcttc aaccaggcgg
    38161 ataatttcac tgcctttcag cgcatgtccg ctcaaacgac gataatccgt cgcgaatccc
    38221 gtgcccgaaa taaaggtttc aatacaacct tgtttaccgc aataacaagg gacttcctcg
    38281 cgataacgca gttcgtcttc gtccatccac ggtagcggat tgtgtcccca ctcacctgcc
    38341 gtgccattgc cgccgatatg cgtccgccca ttgaatgcca cgcccgcgcc gcatcccgtg
    38401 ccgataatca cggcaaatac cgtctgcgct cccgctgccg cgccatctac tgcttctgaa
    38461 accgccagac agttggcatc atttgccagc cgcacttccc gctgcaacct cgcgcttaag
    38521 tctttatcga atggctgacc gttgagccag gttgaattga cattcttcac cacaccggtg
    38581 tagggcgaaa tcgaacctgg aatgcccata cctaccgttc cgcgctgccc tgtcgcctgc
    38641 tccgccatat caaccaacgt ggcgatcgtt tcaatagtct gccggtaatc atcacgcggc
    38701 gtgggcagac gatggcggta caactgctcc cctgcatcgc ccagtgcaat cacttcagtt
    38761 ttggtgccgc ctaaatcgat acctatacgc actgtactct ccttattttt ttcaatatca
    38821 atagcgtaga gacggacaac cggattggca atgcaaggcc gccgacaatt cgttatcatg
    38881 cccgctaaat ttaacgacaa ggccgtggaa attatcatgc tgtggttcaa aaatttaatg
    38941 gtttaccgtc ttagccgcga gatttcgctg cgtgcagaag agatggaaaa acagctagcc
    39001 tcgatggcat ttaccccatg cggcagccag gacatggcga agatgggctg ggttcctccg
    39061 atgggatcgc acagcgatgc gttaacgcac gttgccaatg gtcaaattgt tatctgcgcg
    39121 cgcaaagaag aaaaaatcct cccgtctccg gtgattaaac aggcgctgga agcgaaaatc
    39181 gccaaactgg aagcggaaca ggcgcgtaag ctgaagaaaa ccgaaaaaga ttcgctgaaa
    39241 gacgaagtgc tgcactctct gctgccgcgt gctttcagcc gttttagcca gacaatgatg
    39301 tggctcgaca cggttaacgg tttgattatg gtggactgcg ccagtgccaa aaaagcggaa
    39361 gatacgctgg cattactgcg taaaagcctg gggtcgttac cggttgtacc attgagcatg
    39421 gaaaacccga ttgaactgac gctgaccgaa tgggttcgct ccggtagtgc ggcacagggc
    39481 ttccagctgc ttgatgaagc cgagctgaaa tcgttgctgg aagatggcgg cgtgatccgc
    39541 gcgaagaaac aagatctgac cagcgaagag atcaccaatc acattgaagc cggaaaagtg
    39601 gtgactaaac tggcgctcga ctggcagcag cgcattcagt ttgtgatgtg cgacgatggt
    39661 tcgctcaagc gtcttaagtt ctgcgacgag ctgcgcgatc aaaacgaaga tatcgaccgt
    39721 gaagatttcg cccagcgttt tgatgccgat ttcatcctga tgactggtga actggcagcg
    39781 ttaattcaaa acctgattga aggattaggt ggcgaagcac aacgttaatt gctgattttc
    39841 ctttaatgcc ggatgcgacg cctgccgcgt cttatccggc gtacgaagcc acaccaggca
    39901 tataattatt cgctacggcg agcaataatt tttagcgcag caatattatg cgttttacgc
    39961 cgtaactgat ctaatcgcct catccctatt cgcctgtcag cagcacgcag aatattcact
    40021 aacccccatt tatcgctggt aaattgtttt gttaacagct ccgcttttgg ggtatcaata
    40081 tattcacgca ataaatcaga aattgaacaa atatccgtca ggtaaggata ccagcaagtt
    40141 tcaccgtgaa aattacgcac tccagcgtta cctgcaataa agtctttcgg gtaatcccag
    40201 atcacgtttt tatccaacgt gatccaataa cgcggtaaat cggtacttcc accattttgg
    40261 ctgcgcatgg gataacgggt acagtgaatt tgcaggttga tcgttggcgt cagtaagtca
    40321 tagatctccc gctgtaactt gctccatgga cgttgtgtca ttgtttttcc tcaagccggt
    40381 gacgcgcttc actgcgaatt ttccgcgttg ccccggtttt cactaaatca cgtaactgcg
    40441 aaagatcggg ttgttcgaca aataagagta atgatggatc ctcttttaac cacgccgttt
    40501 tcacatcggc agccagctgt ggattattaa tagccagcga ccgatcctga ggctcagtca
    40561 atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa tgccgtttgg cgcaaatgat
    40621 cattgcgact ttgcagcaat gcacgccacc attcatcatc tgatttatct tttatcgtag
    40681 ccagttccgc aaaaaatacc tggagatctt cactgatatt tatatcaacc ttgatgaatt
    40741 atgaatatat cggaaattga aaaaatcgca gcaaaggaaa tattattatg ggttaaagag
    40801 aggatatttc agaaaaatgc cctcccgttc aaggggaagg cgaggaatta cagatagcgg
    40861 cacagataag aggtaggttc ggcaacttga agatgaaact cactgtgacc gggaacatta
    40921 aacaccgaac cggcttcata cacttgccag tcggtcgcgt caggcagtaa cacattcagc
    40981 gcgccactga ttaccgtcat ctcttccggc tcagcggtgc tgaaggtgta ttcgccttca
    41041 accataacac ccacgctggc gcgaccagtg ctgctgctgg aaaagccgat cgatttcact
    41101 ttgccggaaa agtactcatt actttgaagc ataaactggc cctgtctggt agttgaacat
    41161 ctacttcact ataggggcca gaggcgctgg ctgtcacgca aaattacacc agtaattccg
    41221 cagccagccg tgcaatcaat acgttagaga gcaagacagg aacatcgagc tgtttttgca
    41281 gtaaatcgcg atgacgttgg tgaaatccta aacaatccag catgatgaca tcagcccctt
    41341 ttgccagtaa ttctttccca gcatcaatga ttttttgttc tgaatcatga atggggttac
    41401 ccaatgaaaa taccggcgat ttctgcaaaa tttgccattt ttgcgcctga acgggcagca
    41461 tctcctcaac cggaacgata acccccacct gatgatcttc aacaatagag gaaaccagtg
    41521 gaggcaatat tcgcgacggc tcaagaaaga tcgtattacg cgcagtcata ctactaatgt
    41581 ttgctgtact cattaataaa atgacgtcat aaccctgatt atcgagcact tcaaccacac
    41641 cttgcagatc tcgctccact ttacgacgcg aaacatgggc cagctggttg tcatttaata
    41701 atgtgagaat ggtgtcttcg cctgcttctg gcgcgtactc cgccatcact tcttcacgac
    41761 ttaacttccc cagcaggcta tgatgggaaa tattatcttc gtcaatgtat tccgtcagga
    41821 gcggcaaaac ctcctgcata ggtacaatgc cgatggtcag gatcgccaac gacgcactca
    41881 ttgttcatcc accttacttt tctttcgtcg ttaattaccg ggcaagtgtg aagcaccact
    41941 gctgacactt acttgtgcta caaatgacaa aaagcgtagc agcagacgtg cacggcataa
    42001 tgtaaagatt ccaaatgatt ccagtaatgg aatgttattg tttaatattc taattattag
    42061 aaaaacataa attatgaaaa atgtgacgca gatcccttgt agtggcactc agaatccctt
    42121 ccgagcagtc tgatgcggtc actctttgtc agggtcctca tagcgttttt tcgcatccat
    42181 cgcttcctga gccgtcggat gttcactgat caacgagtct ggtttaggat gatcggctct
    42241 gagttggtac caggttaccg tctgtcctgg ctttcctttt tcaatcgtca ctatatatgc
    42301 ttcacgagga taaggcggtt tcgttggcat cgttcttcct tatttcacgg gatgaacgtt
    42361 aagtataggc gctctaaaat caacaattga tcgtctgtgc cagggcgcta cgaatttcag
    42421 aaatcacctg gctgggttcg tttgttgcgt cgatgataat atgcgcaact tcgcgatata
    42481 gcgcatcgcg ttcttccagc acttcctgaa cttcttcgct cagcggtttt cccgttaagg
    42541 ttggccgtaa atcttcttcc ggtgcagctt gcagtcggtt aaccaggact gatactggcg
    42601 cacacaaata aaccacgatc ccgttatttt gcatgaagtg acgattaaat tccgtcagaa
    42661 taatgccgcc gcctgtagcg ataacggtgg atgccgcagt taccgcttcc agcgccgccg
    42721 tttctctggc gcgaaatccc gcccactctt ccctttcgac gatctccgcg accgtcatat
    42781 tgagctgtga ttgcaaccac tgatcggtat cgacaaaccg acggttaagc gaatcggcaa
    42841 gggccattcc gaccgttgtt ttaccacagc cccgaggccc gatcagaaaa agaggttgtg
    42901 tcatcgtggg ttttccccaa taggtcgcaa tgcggcgaaa gccggtgtca tgagaatagc
    42961 gatcatacca tcaaactagt acaatttcga ttgtaaagaa aaaattccac ttaaagtaaa
    43021 aatctcaata catcccttac tataccaata aatattcaag aatgaagtgt aaataataaa
    43081 ttacatttag ccacgactac gttgcacttc cagccaccac ttctccagct ccgcggcaaa
    43141 ggcctggcgg tcacgttgtg aaaggctatc tggtccgccg gtctggatcc cactggcacg
    43201 taaggtatcc ataaaatcgc gcatcgtcag gcgctcacga atggtcgctg gcgtgtaacg
    43261 ttcgccgcgc gaattaagcg ccgctgcgcc tttctcgatg gcttcagcag ccaaaggtat
    43321 atctgcggtg atcaccaaat cgcccgcttc acactgccgg acaatttcgt tatcggcaac
    43381 gtcgaaacct gccgcgacgc gcagcgtacg aataaatcgc gatggcggca cgcgtaaact
    43441 ctggtttgct accagtacca gcggcatctg catacgttcc gccgcgcgat acaaaatctc
    43501 tttaattaca ttgggacacg cgtcggcatc cacccaaatt gtcataaagt catcctttgt
    43561 taggtaatcc tctattgtgt cgcgcttttg ccttccggca tagttctgtt tatgcttctg
    43621 ccagcgatta tcaaaacaat gaatttcacg gcaggagtga ggcaatggaa aagaaaatcg
    43681 gttttattgg ctgcggcaat atgggaaaag ccattctcgg cggtctgatt gccagcggtc
    43741 aggtgcttcc agggcaaatc tgggtataca ccccctcccc ggataaagtc gccgccctgc
    43801 atgaccagtt cggcatcaac gccgcagaat cggcgcaaga agtggcgcaa atcgccgaca
    43861 tcatttttgc tgccgttaaa cctggcatca tgattaaagt gcttagcgaa atcacctcca
    43921 gcctgaataa agactctctg gtcgtttcta ttgctgcagg tgtcacgctc gaccagcttg
    43981 cccgcgcgct gggccatgac cggaaaatta tccgcgccat gccgaacact cccgcgctgg
    44041 ttaatgccgg gatgacctcc gtaacgccaa acgcgctggt aaccccagaa gataccgctg
    44101 atgtgctgaa tattttccgc tgctttggcg aagcggaagt aattgctgag ccgatgatcc
    44161 acccggtggt cggtgtgagc ggttcttcgc cagcctacgt atttatgttt atcgaagcga
    44221 tggccgacgc cgccgtgctg ggcgggatgc cacgcgccca ggcgtataaa tttgccgctc
    44281 aggcggtaat gggttccgca aaaatggtgc tggaaacggg agaacatccg ggggcactga
    44341 aagatatggt ctgctcaccg ggaggcacca ccattgaagc ggtacgcgta ctggaagaga
    44401 aaggcttccg tgccgcagtg atcgaagcga tgacgaagtg tatggaaaaa tcagaaaaac
    44461 tcagcaaatc ctgatgactt tcgccggacg tcaggccgcc acttcggtgc ggttacgtcc
    44521 ggctttcttt gctttgtaaa gcgccaaatc tgccgatttc aaccactcac gatagtgact
    44581 catttgtggg ttcagcggcg caacccccac actaatccgt aaagttacct gtggcgtatt
    44641 cggcaagcgt aacgtgttta gcccttcacg cacccgtaac atggcggtaa tggcgctctc
    44701 agctggcgta ccggacatga ttactgcaaa ctcatcgccg ccaaaccgac caatcacatc
    44761 gctaccgcgc agggtaattt gtaactgtcg ggtaagcgcc acaatcgctt catcgcccac
    44821 atcatgaccc caggtatcgt tgatgctctt gaaatggtcg atatcgataa tcagtaacgt
    44881 tgcatcgcga ttatgccgcc gacagttatc aaattcattg c
//
LOCUS       DAJTRN010000023        43703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRN010000023 DAJTRN010000000
VERSION     DAJTRN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            43..117
                     /locus_tag="OO328_001311"
     tRNA            43..117
                     /locus_tag="OO328_001311"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Gln,seq:ctg)
     gene            complement(271..1446)
                     /gene="ubiF"
                     /locus_tag="OO328_001312"
     CDS             complement(271..1446)
                     /gene="ubiF"
                     /locus_tag="OO328_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5929939.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1592..3016
                     /gene="miaB"
                     /locus_tag="OO328_001313"
     CDS             1592..3016
                     /gene="miaB"
                     /locus_tag="OO328_001313"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5929940.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3169..4209
                     /locus_tag="OO328_001314"
     CDS             3169..4209
                     /locus_tag="OO328_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5929941.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4206..4673
                     /gene="ybeY"
                     /locus_tag="OO328_001315"
     CDS             4206..4673
                     /gene="ybeY"
                     /locus_tag="OO328_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5929942.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4763..5641
                     /gene="corC"
                     /locus_tag="OO328_001316"
     CDS             4763..5641
                     /gene="corC"
                     /locus_tag="OO328_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5929943.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5666..7204
                     /gene="lnt"
                     /locus_tag="OO328_001317"
     CDS             5666..7204
                     /gene="lnt"
                     /locus_tag="OO328_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5929944.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7601..8509
                     /gene="gltI"
                     /locus_tag="OO328_001318"
     CDS             7601..8509
                     /gene="gltI"
                     /locus_tag="OO328_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5929945.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8679..9419
                     /gene="gltJ"
                     /locus_tag="OO328_001319"
     CDS             8679..9419
                     /gene="gltJ"
                     /locus_tag="OO328_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5929946.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9419..10093
                     /gene="gltK"
                     /locus_tag="OO328_001320"
     CDS             9419..10093
                     /gene="gltK"
                     /locus_tag="OO328_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5929947.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10093..10818
                     /gene="gltL"
                     /locus_tag="OO328_001321"
     CDS             10093..10818
                     /gene="gltL"
                     /locus_tag="OO328_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5929948.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10936..11871
                     /gene="rihA"
                     /locus_tag="OO328_001322"
     CDS             10936..11871
                     /gene="rihA"
                     /locus_tag="OO328_001322"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5929949.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11955..13625
                     /gene="hscC"
                     /locus_tag="OO328_001323"
                     /pseudo
     CDS             11955..13625
                     /gene="hscC"
                     /locus_tag="OO328_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13685..15136)
                     /gene="djlC"
                     /locus_tag="OO328_001324"
     CDS             complement(13685..15136)
                     /gene="djlC"
                     /locus_tag="OO328_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5929950.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15133..15840)
                     /locus_tag="OO328_001325"
     CDS             complement(15133..15840)
                     /locus_tag="OO328_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5929951.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15942..16496
                     /locus_tag="OO328_001326"
     CDS             15942..16496
                     /locus_tag="OO328_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5929952.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16506..17933)
                     /locus_tag="OO328_001327"
     CDS             complement(16506..17933)
                     /locus_tag="OO328_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5929953.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17930..18637)
                     /locus_tag="OO328_001328"
     CDS             complement(17930..18637)
                     /locus_tag="OO328_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5929954.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18801..19778
                     /locus_tag="OO328_001329"
     CDS             18801..19778
                     /locus_tag="OO328_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5929955.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19848..20330)
                     /locus_tag="OO328_001330"
     CDS             complement(19848..20330)
                     /locus_tag="OO328_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5929956.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20565..23147
                     /gene="leuS"
                     /locus_tag="OO328_001331"
     CDS             20565..23147
                     /gene="leuS"
                     /locus_tag="OO328_001331"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5929957.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23162..23743
                     /gene="lptE"
                     /locus_tag="OO328_001332"
     CDS             23162..23743
                     /gene="lptE"
                     /locus_tag="OO328_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5929958.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23743..24774
                     /gene="holA"
                     /locus_tag="OO328_001333"
     CDS             23743..24774
                     /gene="holA"
                     /locus_tag="OO328_001333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5929959.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24776..25417
                     /gene="nadD"
                     /locus_tag="OO328_001334"
     CDS             24776..25417
                     /gene="nadD"
                     /locus_tag="OO328_001334"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5929960.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25441..26052
                     /locus_tag="OO328_001335"
     CDS             25441..26052
                     /locus_tag="OO328_001335"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5929961.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26312..26629
                     /gene="rsfS"
                     /locus_tag="OO328_001336"
                     /gene_synonym="rsfA"
     CDS             26312..26629
                     /gene="rsfS"
                     /locus_tag="OO328_001336"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5929962.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26633..27100
                     /gene="rlmH"
                     /locus_tag="OO328_001337"
     CDS             26633..27100
                     /gene="rlmH"
                     /locus_tag="OO328_001337"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5929963.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27131..29032
                     /gene="mrdA"
                     /locus_tag="OO328_001338"
     CDS             27131..29032
                     /gene="mrdA"
                     /locus_tag="OO328_001338"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5929964.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29035..30147
                     /gene="mrdB"
                     /locus_tag="OO328_001339"
                     /gene_synonym="rodA"
     CDS             29035..30147
                     /gene="mrdB"
                     /locus_tag="OO328_001339"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5929965.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30158..31246
                     /gene="rlpA"
                     /locus_tag="OO328_001340"
     CDS             30158..31246
                     /gene="rlpA"
                     /locus_tag="OO328_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5929966.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31385..32596
                     /gene="dacA"
                     /locus_tag="OO328_001341"
     CDS             31385..32596
                     /gene="dacA"
                     /locus_tag="OO328_001341"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5929967.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32707..32970
                     /locus_tag="OO328_001342"
     CDS             32707..32970
                     /locus_tag="OO328_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5929968.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33071..33712
                     /gene="lipB"
                     /locus_tag="OO328_001343"
     CDS             33071..33712
                     /gene="lipB"
                     /locus_tag="OO328_001343"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5929969.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33971..34924
                     /locus_tag="OO328_001344"
     CDS             33971..34924
                     /locus_tag="OO328_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5929970.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35133..36098
                     /gene="lipA"
                     /locus_tag="OO328_001345"
     CDS             35133..36098
                     /gene="lipA"
                     /locus_tag="OO328_001345"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5929971.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36199..36402)
                     /gene="tatE"
                     /locus_tag="OO328_001346"
     CDS             complement(36199..36402)
                     /gene="tatE"
                     /locus_tag="OO328_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5929972.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36531..37319)
                     /locus_tag="OO328_001347"
     CDS             complement(36531..37319)
                     /locus_tag="OO328_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5929973.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37412..37795
                     /gene="crcB"
                     /locus_tag="OO328_001348"
     CDS             37412..37795
                     /gene="crcB"
                     /locus_tag="OO328_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5929974.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37849..38058)
                     /gene="cspE"
                     /locus_tag="OO328_001349"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37849..38058)
                     /gene="cspE"
                     /locus_tag="OO328_001349"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5929975.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38233..38793)
                     /gene="pagP"
                     /locus_tag="OO328_001350"
     CDS             complement(38233..38793)
                     /gene="pagP"
                     /locus_tag="OO328_001350"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5929976.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39382..40767
                     /gene="dcuC"
                     /locus_tag="OO328_001351"
     CDS             39382..40767
                     /gene="dcuC"
                     /locus_tag="OO328_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5929977.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40808..41149)
                     /locus_tag="OO328_001352"
                     /pseudo
     CDS             complement(40808..41149)
                     /locus_tag="OO328_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41487..41996)
                     /locus_tag="OO328_001353"
     CDS             complement(41487..41996)
                     /locus_tag="OO328_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5929978.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42159..42404
                     /locus_tag="OO328_001354"
     CDS             42159..42404
                     /locus_tag="OO328_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5929979.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42379..>43703
                     /locus_tag="OO328_001355"
     CDS             42379..>43703
                     /locus_tag="OO328_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5929980.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEG"
BASE COUNT        10990 a        10940 c        10897 g        10876 t
ORIGIN      
        1 gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat cgccaagcgg
       61 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
      121 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
      181 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
      241 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
      301 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
      361 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
      421 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
      481 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
      541 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
      601 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
      661 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
      721 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
      781 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
      841 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
      901 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
      961 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
     1021 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
     1081 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
     1141 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
     1201 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
     1261 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
     1321 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
     1381 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
     1441 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
     1501 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
     1561 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
     1621 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
     1681 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
     1741 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
     1801 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
     1861 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
     1921 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
     1981 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
     2041 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
     2101 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
     2161 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
     2221 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
     2281 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
     2341 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
     2401 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
     2461 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
     2521 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
     2581 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
     2641 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
     2701 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
     2761 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
     2821 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
     2881 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
     2941 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
     3001 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
     3061 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
     3121 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
     3181 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
     3241 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
     3301 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
     3361 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
     3421 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
     3481 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
     3541 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
     3601 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
     3661 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
     3721 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
     3781 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
     3841 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
     3901 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
     3961 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
     4021 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
     4081 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
     4141 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
     4201 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
     4261 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
     4321 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
     4381 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
     4441 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
     4501 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
     4561 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
     4621 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
     4681 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
     4741 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
     4801 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
     4861 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
     4921 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
     4981 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
     5041 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
     5101 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
     5161 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
     5221 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
     5281 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
     5341 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
     5401 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
     5461 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
     5521 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
     5581 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
     5641 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
     5701 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
     5761 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
     5821 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
     5881 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
     5941 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
     6001 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
     6061 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
     6121 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
     6181 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
     6241 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
     6301 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
     6361 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
     6421 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
     6481 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
     6541 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
     6601 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
     6661 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
     6721 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
     6781 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
     6841 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
     6901 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
     6961 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
     7021 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
     7081 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
     7141 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
     7201 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
     7261 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
     7321 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
     7381 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
     7441 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
     7501 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
     7561 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
     7621 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
     7681 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
     7741 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
     7801 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
     7861 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
     7921 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
     7981 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
     8041 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
     8101 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
     8161 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
     8221 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
     8281 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
     8341 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
     8401 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
     8461 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
     8521 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
     8581 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
     8641 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
     8701 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
     8761 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
     8821 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
     8881 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
     8941 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
     9001 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
     9061 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
     9121 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
     9181 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
     9241 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
     9301 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
     9361 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
     9421 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
     9481 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
     9541 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
     9601 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
     9661 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
     9721 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
     9781 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
     9841 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
     9901 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
     9961 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    10021 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    10081 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    10141 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    10201 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    10261 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    10321 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    10381 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    10441 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    10501 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    10561 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    10621 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    10681 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    10741 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    10801 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    10861 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    10921 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    10981 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    11041 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    11101 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    11161 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    11221 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    11281 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    11341 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    11401 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    11461 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    11521 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    11581 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    11641 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    11701 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    11761 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    11821 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    11881 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    11941 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    12001 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    12061 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    12121 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    12181 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    12241 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    12301 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    12361 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    12421 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    12481 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    12541 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    12601 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    12661 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    12721 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    12781 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    12841 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    12901 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    12961 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    13021 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    13081 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    13141 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    13201 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    13261 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    13321 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    13381 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    13441 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    13501 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    13561 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    13621 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    13681 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    13741 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    13801 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    13861 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    13921 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    13981 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    14041 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    14101 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    14161 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    14221 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    14281 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    14341 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    14401 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    14461 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    14521 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    14581 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    14641 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    14701 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    14761 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    14821 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    14881 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    14941 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    15001 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    15061 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    15121 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    15181 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    15241 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    15301 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    15361 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    15421 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    15481 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    15541 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    15601 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    15661 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    15721 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    15781 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    15841 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    15901 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    15961 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    16021 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    16081 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    16141 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    16201 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    16261 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    16321 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    16381 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    16441 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    16501 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    16561 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    16621 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    16681 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    16741 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    16801 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    16861 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    16921 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    16981 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    17041 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    17101 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    17161 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    17221 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    17281 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    17341 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    17401 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    17461 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    17521 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    17581 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    17641 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    17701 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    17761 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    17821 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    17881 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    17941 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    18001 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    18061 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    18121 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    18181 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    18241 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    18301 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    18361 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    18421 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    18481 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    18541 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    18601 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    18661 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    18721 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    18781 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    18841 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    18901 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    18961 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    19021 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    19081 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    19141 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    19201 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    19261 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    19321 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    19381 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    19441 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    19501 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    19561 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    19621 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    19681 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    19741 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    19801 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    19861 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    19921 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    19981 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    20041 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    20101 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    20161 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    20221 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    20281 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    20341 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    20401 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    20461 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    20521 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    20581 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    20641 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    20701 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    20761 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    20821 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    20881 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    20941 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    21001 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    21061 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    21121 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    21181 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    21241 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    21301 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    21361 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    21421 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    21481 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    21541 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    21601 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    21661 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    21721 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    21781 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    21841 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    21901 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    21961 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    22021 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    22081 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    22141 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    22201 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    22261 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    22321 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    22381 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    22441 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    22501 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    22561 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    22621 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    22681 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    22741 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    22801 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    22861 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    22921 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    22981 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    23041 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    23101 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    23161 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    23221 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    23281 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    23341 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    23401 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    23461 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    23521 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    23581 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    23641 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    23701 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    23761 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    23821 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    23881 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    23941 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    24001 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    24061 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    24121 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    24181 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    24241 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    24301 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    24361 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    24421 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    24481 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    24541 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    24601 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    24661 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    24721 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    24781 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    24841 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    24901 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    24961 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    25021 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    25081 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    25141 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    25201 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    25261 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    25321 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    25381 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    25441 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    25501 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    25561 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    25621 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    25681 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    25741 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    25801 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    25861 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    25921 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    25981 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    26041 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    26101 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    26161 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    26221 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    26281 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    26341 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    26401 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    26461 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    26521 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    26581 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    26641 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    26701 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    26761 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    26821 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    26881 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    26941 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    27001 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    27061 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    27121 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    27181 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    27241 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    27301 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    27361 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    27421 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    27481 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    27541 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    27601 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    27661 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    27721 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    27781 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    27841 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    27901 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    27961 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    28021 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    28081 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    28141 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    28201 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    28261 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    28321 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    28381 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    28441 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    28501 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    28561 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    28621 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    28681 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    28741 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    28801 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    28861 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    28921 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    28981 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    29041 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    29101 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    29161 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    29221 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    29281 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    29341 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    29401 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    29461 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    29521 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    29581 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    29641 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    29701 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    29761 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    29821 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    29881 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    29941 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    30001 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    30061 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    30121 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    30181 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    30241 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    30301 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    30361 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    30421 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    30481 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    30541 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    30601 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    30661 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    30721 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    30781 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    30841 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    30901 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    30961 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    31021 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    31081 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    31141 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    31201 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    31261 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    31321 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    31381 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    31441 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    31501 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    31561 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    31621 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    31681 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    31741 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    31801 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    31861 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    31921 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    31981 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    32041 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    32101 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    32161 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    32221 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    32281 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    32341 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    32401 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    32461 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    32521 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    32581 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    32641 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    32701 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    32761 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    32821 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    32881 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    32941 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    33001 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    33061 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    33121 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    33181 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    33241 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    33301 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    33361 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    33421 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    33481 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    33541 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    33601 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    33661 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    33721 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    33781 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    33841 aactcacccc attgattaac ctattgaaat ggttgattat ggtgagcaaa agcgttccgt
    33901 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    33961 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    34021 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    34081 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    34141 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    34201 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    34261 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    34321 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    34381 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    34441 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    34501 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    34561 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    34621 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    34681 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    34741 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    34801 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    34861 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    34921 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    34981 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    35041 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    35101 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    35161 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    35221 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    35281 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    35341 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    35401 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    35461 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    35521 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    35581 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    35641 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    35701 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    35761 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    35821 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    35881 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    35941 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    36001 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    36061 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    36121 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    36181 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    36241 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    36301 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    36361 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    36421 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    36481 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    36541 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    36601 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    36661 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    36721 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    36781 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    36841 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    36901 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    36961 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    37021 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    37081 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    37141 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    37201 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    37261 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    37321 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    37381 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    37441 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    37501 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    37561 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    37621 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    37681 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    37741 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    37801 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    37861 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    37921 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    37981 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    38041 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    38101 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    38161 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    38221 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    38281 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    38341 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    38401 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    38461 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    38521 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    38581 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    38641 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    38701 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    38761 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    38821 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    38881 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    38941 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    39001 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    39061 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    39121 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    39181 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    39241 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    39301 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    39361 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    39421 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    39481 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    39541 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    39601 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    39661 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    39721 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    39781 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    39841 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    39901 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    39961 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    40021 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    40081 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    40141 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    40201 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    40261 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    40321 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    40381 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    40441 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    40501 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    40561 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    40621 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    40681 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    40741 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    40801 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    40861 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    40921 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    40981 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    41041 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    41101 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    41161 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    41221 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    41281 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    41341 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc atcaaatgac
    41401 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    41461 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    41521 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    41581 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    41641 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    41701 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    41761 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    41821 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    41881 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    41941 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    42001 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    42061 gatataaacc ggtttggttt atttggtaat gtataaaaca gataatcaag acgaattatt
    42121 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    42181 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    42241 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    42301 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    42361 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    42421 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    42481 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    42541 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    42601 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    42661 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    42721 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    42781 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    42841 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    42901 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    42961 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    43021 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    43081 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    43141 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    43201 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    43261 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    43321 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    43381 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    43441 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    43501 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    43561 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    43621 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    43681 tttctgaaaa cctttgccga agg
//
LOCUS       DAJTRN010000024        43302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRN010000024 DAJTRN010000000
VERSION     DAJTRN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO328_001356"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO328_001356"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5929981.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO328_001357"
     CDS             complement(1672..2730)
                     /locus_tag="OO328_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5929982.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO328_001358"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO328_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO328_001359"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO328_001359"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5929983.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO328_001360"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO328_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5929984.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO328_001361"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO328_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5929985.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO328_001362"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO328_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5929986.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO328_001363"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO328_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5929987.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO328_001364"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO328_001364"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5929988.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO328_001365"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO328_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5929989.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO328_001366"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO328_001366"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5929990.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO328_001367"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO328_001367"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5929991.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO328_001368"
     CDS             complement(17699..18322)
                     /locus_tag="OO328_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5929992.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO328_001369"
     CDS             18619..18894
                     /locus_tag="OO328_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5929993.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO328_001370"
     CDS             complement(18971..19213)
                     /locus_tag="OO328_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5929994.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO328_001371"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO328_001371"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5929995.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO328_001372"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO328_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5929996.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO328_001373"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO328_001373"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5929997.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO328_001374"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO328_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO328_001375"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO328_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5929998.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO328_001376"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="OO328_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO328_001377"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO328_001377"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5929999.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO328_001378"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO328_001378"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5930000.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO328_001379"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO328_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OO328_001380"
     CDS             complement(30355..31602)
                     /locus_tag="OO328_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5930001.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OO328_001381"
     CDS             complement(31624..31950)
                     /locus_tag="OO328_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5930002.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO328_001382"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO328_001382"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5930003.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OO328_001383"
     CDS             33338..34594
                     /locus_tag="OO328_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5930004.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OO328_001384"
     CDS             34709..35035
                     /locus_tag="OO328_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5930005.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OO328_001385"
     CDS             complement(35176..36414)
                     /locus_tag="OO328_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5930006.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OO328_001386"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OO328_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5930007.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OO328_001387"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OO328_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OO328_001388"
     tRNA            complement(40322..40397)
                     /locus_tag="OO328_001388"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="OO328_001389"
     tRNA            complement(40437..40512)
                     /locus_tag="OO328_001389"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="OO328_001390"
     CDS             40733..41092
                     /locus_tag="OO328_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5930008.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="OO328_001391"
     CDS             41094..41486
                     /locus_tag="OO328_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5930009.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO328_001392"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO328_001392"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5930010.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="OO328_001393"
     tRNA            43212..43287
                     /locus_tag="OO328_001393"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
BASE COUNT        11528 a        10780 c        10036 g        10958 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctt aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gt
//
LOCUS       DAJTRN010000025        42725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRN010000025 DAJTRN010000000
VERSION     DAJTRN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO328_001394"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO328_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5930011.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO328_001395"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO328_001395"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5930012.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO328_001396"
     CDS             complement(3736..4092)
                     /locus_tag="OO328_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5930013.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO328_001397"
     CDS             complement(4096..4512)
                     /locus_tag="OO328_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5930014.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO328_001398"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO328_001398"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5930015.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO328_001399"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO328_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO328_001400"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO328_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5930016.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO328_001401"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO328_001401"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5930017.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO328_001402"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO328_001402"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5930018.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO328_001403"
     CDS             10521..11504
                     /locus_tag="OO328_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5930019.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO328_001404"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO328_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5930020.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO328_001405"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO328_001405"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5930021.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO328_001406"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO328_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO328_001407"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO328_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5930022.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO328_001408"
     CDS             complement(15306..15515)
                     /locus_tag="OO328_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5930023.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO328_001409"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO328_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5930024.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO328_001410"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO328_001410"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5930025.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO328_001411"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO328_001411"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5930026.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO328_001412"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO328_001412"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5930027.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO328_001413"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO328_001413"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5930028.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO328_001414"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO328_001414"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5930029.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO328_001415"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO328_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO328_001416"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO328_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5930030.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO328_001417"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO328_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5930031.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO328_001418"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO328_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5930032.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO328_001419"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO328_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5930033.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO328_001420"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO328_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5930034.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO328_001421"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO328_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5930035.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO328_001422"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO328_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5930036.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO328_001423"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO328_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5930037.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO328_001424"
     CDS             complement(34461..34604)
                     /locus_tag="OO328_001424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930038.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO328_001425"
     CDS             34605..34883
                     /locus_tag="OO328_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930039.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO328_001426"
     CDS             complement(34930..37026)
                     /locus_tag="OO328_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5930040.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAISWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO328_001427"
     CDS             complement(37026..37763)
                     /locus_tag="OO328_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5930041.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO328_001428"
     CDS             complement(37760..38398)
                     /locus_tag="OO328_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5930042.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO328_001429"
     CDS             complement(38512..38754)
                     /locus_tag="OO328_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930043.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO328_001430"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO328_001430"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5930044.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..>42725
                     /gene="lysC"
                     /locus_tag="OO328_001431"
     CDS             41391..>42725
                     /gene="lysC"
                     /locus_tag="OO328_001431"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5930045.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHS"
BASE COUNT        10629 a        11005 c        10488 g        10603 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagctaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagt
//
LOCUS       DAJTRN010000026        40200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRN010000026 DAJTRN010000000
VERSION     DAJTRN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..955)
                     /locus_tag="OO328_001432"
     CDS             complement(<1..955)
                     /locus_tag="OO328_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5930046.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAG"
     gene            1201..1827
                     /gene="gstB"
                     /locus_tag="OO328_001433"
     CDS             1201..1827
                     /gene="gstB"
                     /locus_tag="OO328_001433"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5930047.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1824..2938)
                     /gene="yliI"
                     /locus_tag="OO328_001434"
                     /pseudo
     CDS             complement(1824..2938)
                     /gene="yliI"
                     /locus_tag="OO328_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="OO328_001435"
     CDS             complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="OO328_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5930048.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3645..4970
                     /gene="rimO"
                     /locus_tag="OO328_001436"
     CDS             3645..4970
                     /gene="rimO"
                     /locus_tag="OO328_001436"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5930049.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5017..6345)
                     /locus_tag="OO328_001437"
     CDS             complement(5017..6345)
                     /locus_tag="OO328_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5930050.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6353..8695)
                     /locus_tag="OO328_001438"
                     /pseudo
     CDS             complement(6353..8695)
                     /locus_tag="OO328_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8873..9784)
                     /gene="gsiD"
                     /locus_tag="OO328_001439"
     CDS             complement(8873..9784)
                     /gene="gsiD"
                     /locus_tag="OO328_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5930051.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9787..10707)
                     /gene="gsiC"
                     /locus_tag="OO328_001440"
     CDS             complement(9787..10707)
                     /gene="gsiC"
                     /locus_tag="OO328_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5930052.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10725..12263)
                     /gene="gsiB"
                     /locus_tag="OO328_001441"
     CDS             complement(10725..12263)
                     /gene="gsiB"
                     /locus_tag="OO328_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5930053.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12283..14154)
                     /gene="gsiA"
                     /locus_tag="OO328_001442"
     CDS             complement(12283..14154)
                     /gene="gsiA"
                     /locus_tag="OO328_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5930054.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14141..15106)
                     /gene="iaaA"
                     /locus_tag="OO328_001443"
     CDS             complement(14141..15106)
                     /gene="iaaA"
                     /locus_tag="OO328_001443"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5930055.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15310..16545
                     /gene="moeA"
                     /locus_tag="OO328_001444"
     CDS             15310..16545
                     /gene="moeA"
                     /locus_tag="OO328_001444"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5930056.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16545..17294
                     /gene="moeB"
                     /locus_tag="OO328_001445"
     CDS             16545..17294
                     /gene="moeB"
                     /locus_tag="OO328_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5930057.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17473..18135)
                     /gene="fsa"
                     /locus_tag="OO328_001446"
     CDS             complement(17473..18135)
                     /gene="fsa"
                     /locus_tag="OO328_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5930058.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18266..19165
                     /locus_tag="OO328_001447"
     CDS             18266..19165
                     /locus_tag="OO328_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5930059.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19171..21603
                     /locus_tag="OO328_001448"
     CDS             19171..21603
                     /locus_tag="OO328_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5930060.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21749..22564
                     /gene="ybiV"
                     /locus_tag="OO328_001449"
     CDS             21749..22564
                     /gene="ybiV"
                     /locus_tag="OO328_001449"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5930061.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22716..23981
                     /locus_tag="OO328_001450"
     CDS             22716..23981
                     /locus_tag="OO328_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5930062.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24222..25814)
                     /locus_tag="OO328_001451"
     CDS             complement(24222..25814)
                     /locus_tag="OO328_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5930063.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26033..26953
                     /gene="ldtB"
                     /locus_tag="OO328_001452"
     CDS             26033..26953
                     /gene="ldtB"
                     /locus_tag="OO328_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5930064.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27011..28129)
                     /locus_tag="OO328_001453"
     CDS             complement(27011..28129)
                     /locus_tag="OO328_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5930065.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="OO328_001454"
     CDS             complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="OO328_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5930066.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28779..28907
                     /gene="mntS"
                     /locus_tag="OO328_001455"
     CDS             28779..28907
                     /gene="mntS"
                     /locus_tag="OO328_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5930067.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29179..30762
                     /gene="opgE"
                     /locus_tag="OO328_001456"
     CDS             29179..30762
                     /gene="opgE"
                     /locus_tag="OO328_001456"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5930068.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="OO328_001457"
     CDS             complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="OO328_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5930069.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31378..31443)
                     /locus_tag="OO328_001458"
     CDS             complement(31378..31443)
                     /locus_tag="OO328_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930070.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31678..32565
                     /gene="rhtA"
                     /locus_tag="OO328_001459"
     CDS             31678..32565
                     /gene="rhtA"
                     /locus_tag="OO328_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5930071.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32864..33367
                     /gene="dps"
                     /locus_tag="OO328_001460"
                     /gene_synonym="pexB"
     CDS             32864..33367
                     /gene="dps"
                     /locus_tag="OO328_001460"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5930072.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33771..34517
                     /gene="glnH"
                     /locus_tag="OO328_001461"
     CDS             33771..34517
                     /gene="glnH"
                     /locus_tag="OO328_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5930073.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34656..35315
                     /gene="glnP"
                     /locus_tag="OO328_001462"
     CDS             34656..35315
                     /gene="glnP"
                     /locus_tag="OO328_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5930074.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35312..36034
                     /gene="glnQ"
                     /locus_tag="OO328_001463"
     CDS             35312..36034
                     /gene="glnQ"
                     /locus_tag="OO328_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5930075.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36295..38519
                     /gene="ybiO"
                     /locus_tag="OO328_001464"
                     /pseudo
     CDS             36295..38519
                     /gene="ybiO"
                     /locus_tag="OO328_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38516..39442)
                     /gene="rlmF"
                     /locus_tag="OO328_001465"
     CDS             complement(38516..39442)
                     /gene="rlmF"
                     /locus_tag="OO328_001465"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5930076.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39718..39978
                     /locus_tag="OO328_001466"
     CDS             39718..39978
                     /locus_tag="OO328_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5930077.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
BASE COUNT        10003 a        10622 c         9787 g         9788 t
ORIGIN      
        1 agcccgcttc gcctgccccg agattcactt cgatcttatc accaaaccag acgcgctgag
       61 tcacaaaggt ggcatcaggt ttaattggcg tcacagtttc aaagaagcgg aaaccccagg
      121 tcaataattt ctcagactca ttaaaacgga tacggtcagt tttcgccccc agcactacgg
      181 agattaaacg catatcaccc tgggtagccg aagcaaccag attatatccc gcacctgccg
      241 tggttcctgt cttcatgcca tcaacattca gattgctgct ccacagcaga cggttacggt
      301 taggctgacg aattttgttg aaggtgaact ctttctcttt atgaatggcg tactcttccg
      361 gcacatcgtg gatcaatgct ttacccagca atgccatatc gcgcgcggta ctgaactgcc
      421 ccggcgcatc caggccgtgc accgtctgga aggtagtgtt ggtcagcccc agttttttgg
      481 cataaccatt catcagacca ataaaggact cctggctccc ggcaacgtaa tctgccagcg
      541 caatacaggc gtcattaccg gactggataa tcacaccttt gttcaagtct gccaccgaaa
      601 cctgatcgcc cggtttgagg aacattaccg atgaaccacg cagtgccgga tttcccgtcg
      661 cccaggcatc tttaccgacc gtcaccatat cggtgagttt aatcttatcg gccttaagcg
      721 cctgcccaac cacatagctg gtcatgattt tagtcaggct cgcaggatcc agtttctcat
      781 ccgcattgcc ttctgccagc actttaccgc tggcgtaatc cattaaaatc catgcacgcg
      841 catccacgct cggcgcttca acggtttgtt cggccgcgaa tgccgttggg gcaaaaagga
      901 ataaaaatgc agaacccgct gcaagaccac gaaggagaga ggagtattgc gtcataaatg
      961 ccacccgagt atccattcaa aaaaatacgt tatatcgccg tatgataagt tcggtgatta
     1021 ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa
     1081 tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa
     1141 ctttacttca ccatcggagt cctgttcagt ctgtggctgc ctgtcggaaa aggggtgagt
     1201 atgattacgc tgtggggtcg gaataattca actaacgtaa aaaaagtatt gctgacgctc
     1261 gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac
     1321 gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa
     1381 agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg
     1441 caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac
     1501 tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca
     1561 ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg
     1621 tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga cgagtttggt
     1681 gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg
     1741 acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc
     1801 aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg
     1861 gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc acgaattcgc
     1921 tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc
     1981 acaatgacat ctttatcttt cagctcccaa taaataattt ttgctgccac tgggggaatt
     2041 tatcgctgtt atagaaggcc atgccgctca cagcgggcga atctttccag taaaaaacag
     2101 gttgctcggt cccggcgacg atctcccctt tcgcttccgg tatcttaaag cctgaatagt
     2161 tgattcccca ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt
     2221 catcaccacc gcgcgggcca tgttcattca gccacagtgc attactccac ggattcatcg
     2281 ccattccttg cggattacga atgccataag accagatctc ggcgcgcgca ccggattcct
     2341 ttataaaagg attatcatcc gggatttcgc cctggtcggt cagacgcacc agtttgccct
     2401 gtaatttatc cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa
     2461 gataaccttt accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg
     2521 gcatctggcg gaagacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc
     2581 cataacccac agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga
     2641 tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt
     2701 gcgcccaaac gtccggaact ccggaaagcg gcgcagataa tccttttcct gcttgccagt
     2761 gacgcaactc gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca
     2821 gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagccg
     2881 cccagagagc ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa
     2941 cgcctccgga ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt
     3001 tacaaaaact taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc
     3061 aagcatggcg tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc
     3121 aagcttatcg gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca
     3181 tactgcggct gtcgctaatg cttcttcagc atgatgaaga gcactccact cttcctgatc
     3241 cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt
     3301 atctcgtaga tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac
     3361 gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct
     3421 gtcaacgaac atacttcgat cctcctcttt ccagccttgt tgcaattaaa tagtatggct
     3481 attttttgag caaaatgctg tccaggatca attcttcacg ccgaattaac aaaatttaca
     3541 tttccttcgc ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag
     3601 aatccctgcc ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc
     3661 cccagccgaa aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc
     3721 gtattctcac cgaactccgc actgaaggtt atgacgtggt accgagctat gacgatgcga
     3781 acatggtgat cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag
     3841 ccattggtga agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa
     3901 aagaagatca gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct
     3961 atgagcaggt tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc
     4021 tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa
     4081 tttcggaagg ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgacc
     4141 tgatgagccg tccgattggc gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg
     4201 ttaaagagat tctggtgatc tcgcaggata cttccgccta tggcgttgat gttaaacatc
     4261 gtactggctt ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt
     4321 tatcgaaact ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg
     4381 acgtcatccc actgatggca gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc
     4441 acgccagccc gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg
     4501 cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg
     4561 tcggcttccc tggcgagacg gaagaagatt tccagatgct actcgacttc ctgaaagaag
     4621 cacgtctgga tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg
     4681 ccctgcctga ccaggttccg gaagaagtga aagaagaacg ctggaaccgt ttcatgcagc
     4741 tgcagcagca gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga
     4801 ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa
     4861 tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg
     4921 tgaaagtcga gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg
     4981 ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgtgccct
     5041 gccgtttttc ttactcttat acagccgctc atccgccagc attttcaagc gttcgacggt
     5101 agtacattca tttgaatgcg ctacaccgat actggtgctt aaattaatgc ttcccacctg
     5161 gaaatgaacg atttctttta gtatcgatag caactcttcc agcgtgccgc cagtataaat
     5221 caccgcaaat tcgtcacctc cgaagcggta gactttacct ttatctaccg ggatctgtga
     5281 tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc
     5341 gttaatgttt ttgaatttat cgacgtcaaa cagacacaaa taaccgcctt tttccggcag
     5401 cgcttttaaa tcttcatcaa aggattttct gcccattgct ttggtcaggt cgtccaggaa
     5461 cgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc
     5521 atccagtacg cgttcaaaat ggttattaat gatggcttct ttctccacaa taccacgatt
     5581 aatcactctg cgtaccagat agaggcaact ggcacccaat atcactgcca ccaatgcggc
     5641 aaagaaaata atcaatgtac aaacccgcaa ataaacatct tttttatacg caaatacgta
     5701 caatccggtc ggcaaatagg cgaagtgctc catcgcttcc gaaacggtga agatggcgtt
     5761 tttagcatta tattcatcac cggtcaccgg tttaccgttc ttaaatagag agaacatcag
     5821 gttctcgctg tccatcacca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa
     5881 aaatatcaga ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgtc
     5941 aaaagagata aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc
     6001 acaataattt ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg
     6061 accgacatcg tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac
     6121 caacagctgt tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg
     6181 atattcagca atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata
     6241 cacccgttcc tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa
     6301 aatcagcaga ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt
     6361 ttactaattt tccactttta tcccaagcgg agaatggcat gggtttctgg tacagatacc
     6421 cctgagcctg aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc
     6481 cttccgcaat caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt
     6541 ttcccgcagt accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat
     6601 taatttcata aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc
     6661 gtaccccttg tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga
     6721 tcacggtttc agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga
     6781 tggtggtaaa acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat
     6841 gcagtaagaa atcatccttc agcgacgcac gttgactaaa atgtctgaat taacccattg
     6901 ccgttttaat aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa
     6961 tgaacttcag cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg
     7021 cgctcagaat ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc
     7081 caatttccat tgcatggcga atattacgcg caatccagat attctcttct atttcgttga
     7141 gtttcgcgtc atcacagaaa ataaactcgg agaacatatg gtcacggata ctggaaatcg
     7201 ccagtttcag tttttctgcg taactccagg cgttttcccc ctgcaaaggt tcacaaatgg
     7261 cagacttatg gatgcgcaga ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg
     7321 ctattttacg ggtaaatgtc tgtacatttt cccgacacag aagtaaatag agatcggcat
     7381 tgaatgtcac gatatcgcag cattgcgcat atttctcggc aattcgctga cacaatttcc
     7441 tgacgatttt attgcgttca tcaacaccaa acaccgcctc cacggcttcc agaccacaca
     7501 gtttgatgat ccccacagcc agatactgat tattgttgga ctcaataatc gccatatttt
     7561 taataaaacc gctttcctga tcgtaagaga tcagtgagag caatttattt gaccgttcaa
     7621 tttcggcatc tttcatttcc gcgatcgaat ttttgatgct gataatctct ttcgcatcgc
     7681 cttcgcgaat tttcagagcc tcgatctgat ccgttgagtc gggcaaaaac gccagcgtat
     7741 taaccagctc ggtcatttca gaaaattgcc gacggaacac acgagtggtc atgaaatata
     7801 ccaccagcaa cacgataata atgaacgcaa acggtagata aaaaagttta tctgatgcgt
     7861 tcaataaatt accgccagga taataaaaat agatttcgaa caatgcatta tttggcactg
     7921 ctgatgtctt tgcaatatac tccgtatcga cagaatcaat aatattataa ttaccttcgc
     7981 tattataaat aatcagtttt tcacgagcga gttgattctt ggcaatgatc tcatgacctt
     8041 gtttcaaaaa tagaacttca tcattagcgc tgatttgatc aagatactcg ttcagatacc
     8101 gccagtttaa atcgacatga attttatcta ccgctaacaa atcgtgatct ttgccgttga
     8161 tacgtagcgt aaaagagata cattttttac ccggacgatc aaagcaattg tatgccggat
     8221 tccagctgtt gttagagtca tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat
     8281 cggcaaaaat aacccgccct gactcgtcga caagcggtcc atttacttgt tcctgatgat
     8341 aaacaacgat tttatcattc gccctccgcg aaaagagata atatttatta tcatcaagta
     8401 ccagtcccac cgagtctaaa aatggcatca tttccatttt cagaatcacg gcgcgttgaa
     8461 tctcctctgt attttttaat aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa
     8521 tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta tcggcacgtt
     8581 ttaccgcgcg ttccgatacc agttgatagt taatcatgaa aaaaagacca ataaatgaca
     8641 gtgctgccaa taaaaataaa attatcagcc ttatcttttc gtataaactc agcatgttaa
     8701 tacatttaac aaaataacta tctgaacgct gtgccgctgc aaaaatagtc ccttgctgcg
     8761 gctttttaac caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag
     8821 ggcaataatt tcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt
     8881 taattttcgg atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg
     8941 tcagaaaaat agccagggcc ggaaaaacag cgacatgcgg cgcgataacc atatccgctc
     9001 gtgcctcatt gagcattgct ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa
     9061 atgacaggct ggcggcagag ataatcgagg tgccaatgcg catggtgaaa aacaccacga
     9121 tagaagagac ggtcccaggc aggatatgac gcaataaaat ggtcatatcg ctggcaccaa
     9181 tactgcgtgc tgactcaata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca
     9241 ggcgggcaaa cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc
     9301 ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacagcacat
     9361 cgcaaatgcg catgatcagc cgatcccacc agccttcata atatccagcc aacaagccca
     9421 gcaacgtccc tatcgccaca ccgataaaca cggcaaacac gcccgccgcc agcgagattt
     9481 gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt
     9541 gctgcaaaga aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg
     9601 gagcgatcca gcgtgcaaaa atggccacca caatcaataa aataacgaat aatgcggcgg
     9661 tcatcgccat atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgtacct
     9721 ggtcaggttt gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc
     9781 gcatccttac ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa
     9841 gttgataaga ataaattcca gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc
     9901 gcgcatttct acagagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc
     9961 caccacgata gaaccaccga gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg
    10021 gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc
    10081 cctcgcggta cgcatataat cttcgcttaa aacgtcgaca aatgacgcac gggtaaagcg
    10141 cgccatcacg gcggccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca
    10201 actgtctgct cccacagtag gcagccagcc cagttcaacg gagaatacct gaattaaaaa
    10261 catccccagc gcaaatgccg gaaacgagat ccccgacacc gcaatggtca tactcaatcg
    10321 atccggccaa cgattacgcc agacggcggc gataattccc gccgccatac caaatatcac
    10381 cgcccagacc atactggtta tggtcagcca tagcgttggc ataaagcggc tggcaatctc
    10441 atcggcaacc ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct
    10501 gatatagtgc cagaactggt gatacagcgg ctgatccaac cccagctgct gacgcaccag
    10561 ttctataacc tgcgcatcag cttcgggccc ggcaatcaat cgcgccggat cgccgggcag
    10621 catgtggaca aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag
    10681 taatcgtttg ataacgtaat aaagcattcc actccctgcg ttgcttattg caaatccgcg
    10741 tcttcaaagc tgaagccggt atctggcatg atccaaaaac cggtcaggtt tttactgtgt
    10801 gccgacacca gtttttctac caccagcggg atccacggcg attcttgcca gatgatatcc
    10861 tgcgccgcct tatataagcg ggtcttttcc gccggatcat tcgttttcag tgcctgagcc
    10921 aggaagtcat ccacctgttt attgctgtaa aacgcggtat taaacagcgt cggtggccag
    10981 ttttgcgagg caaacagcgg cgatagtgcc cagtcggctt cgccggttga agccgaccag
    11041 ccagtgtaga acatccgcac gccactctct ttttgccctt taccttcaac ttctgccgcc
    11101 cgctgtccgg catccatcgc ggtcacctgg gctttaatcc cgacctgcgc taactgctgc
    11161 tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta
    11221 ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc gcgctttcac tggatcgtaa
    11281 ggccacggtt tataactttg cgcgtaggcg atacttggcg gtaccacacc agtagctgga
    11341 gttgcatagc ccgcaaaggc aactttcacc agcgccggac ggttaatggc gtaattcagc
    11401 gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc
    11461 tgcataattg acggactggc catcaactcg atatttttgt ttttctccag cagtgtggcc
    11521 tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg
    11581 gtgttgttat ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc
    11641 cagtaacccg cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca
    11701 tacggtccgg ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgtc
    11761 ggtgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga gaacggctgt
    11821 ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg ttttggcaat attcttatac
    11881 aggttatagc gtttaagatg attcgccgga tcgctggccc ggtccagatt cgcttttacc
    11941 gccactgcgt tgaaatcggt gccatcctgg aatttaattc cttcccgcaa tttcacggtg
    12001 taagtaatgc cgtcatcgga aacggtataa ctctccgcca gcacgttttt cagtttcatc
    12061 tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg
    12121 tcattcgcgt catacggatc gagcgtggtg aaattcgatc ctaccgccac caccacatct
    12181 ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca
    12241 ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc
    12301 gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acgagacggc
    12361 tgccacctct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg
    12421 ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata
    12481 aggatgctgc gggttttcga agaccgcgca ccgtgaacca atttcaacaa tttgcccgag
    12541 atacatcacc gccacacgat gactaatccg ctctaccaca gccatatcgt gggagataaa
    12601 cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa tctgcccgcg
    12661 aatagaaaca tccagcgccg aaacggcttc gtcggcaatg atcacttttg gattcaatgc
    12721 caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat gcgggtaacg
    12781 ccaggcatgt tcaggtaaca ggcccacgcg ctccagcaac cacgcaacgc gtgcagccgc
    12841 gtctttacct ggcaataaac cgtgtacacg cagcggttcg ataatcgaat caccgatggt
    12901 ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat cccggcgcaa
    12961 tgcctgaagt ttgccaggtg acaaggtatc gattcgctga ccgttaaaga taatttcgcc
    13021 gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta gtggatttac cgctgccaga
    13081 ctcgcccacc agcgatagcg tttcgccagg ccagagatca aaactgactt tctcaacggc
    13141 atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac gggtgacaag
    13201 attacgcacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga tggggggttc
    13261 ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat ctaacccttt
    13321 catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtgtaaggat gttgcggtgc
    13381 atgaaaaatc tgttcgacgg tacccgtttc caccgcctcg ccctgataca tcaccagtac
    13441 ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa cgcccatcga
    13501 catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag tgacatccag
    13561 cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg ccatcgcaat
    13621 catcactcgc tggcgcatcc cgccagagag ttgatgcgaa taacgtgaaa gaatggtttg
    13681 tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg
    13741 actggcgttc tgatgcagac gaattgattc ggcaatctgt tcacccacag taaatactgg
    13801 gttcagcgat gtcatcggct cctgaaatat catcgccata tccgcaccgc gcacatggcg
    13861 catttgtgca gcgctctgct cgctaagttc aatcacatca cgactgcgcc gccgcaacag
    13921 cattttatcg cactgtacta aaccgcccgc ctgttccaac agacgcatca atgccagcgc
    13981 agtcactgac ttaccggagc cggattcgcc tacaattgcc agcgtctcac cgcgttgcag
    14041 actaaaagag agattgcgga ccgcagctat tttctgctgg tcctgcataa aggcaatatt
    14101 aagattttca accgccagca cattaccggc atcaagttca tcactgtgtg gcaacggtgt
    14161 cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat
    14221 acattccttc ggtgttaaac ggtagcgcga cattcccttc atggtcgata gcgattaagc
    14281 caccgctacc gccaagcgca gggagttttt ccattactac ccgctcgcag gcttccgcga
    14341 gacttaatcc gccgtaatcc attaacgcgg cgatgtcata tgccgccagc gcgcggatga
    14401 agacttcgcc tgtgccggta caagaaaccg ccacactggc gttattggcg tagcatccgg
    14461 cacccactaa gggactatcg ccaactcgtc cgggtaattt attggtcatt ccgcctgtgg
    14521 acgtggctgc cgccagattg ccgtctaaat ccaacgccac ggcccccacg gtgcccattt
    14581 tctgtttttc atccagtggc gcaccgctat ggtcgaggac tgttgcccct tcctcgcgcg
    14641 ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat ctccggtgag acgcgctcca
    14701 tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca tgcgggcttt
    14761 gctccatcac cagccgggcg gcaagaaccg gattacgcag atgactaacg cccgccaccg
    14821 caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg gtttcatcac
    14881 gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc agacgcaccg
    14941 cttccgtcac cacatccagc gcactttcgc cggcttccag cattttctgc ccggtttcaa
    15001 caatggcaga caacgcctcg atgtagcgta attcctgttg cagactcatc tgcgcgcggc
    15061 taattgcacc tgcgccacca tgaattgcaa tgactgcttt gcccatagta ttcgtccatt
    15121 aagggtggct cgccactttt gctataaata tcagaatttt tatgaattac tcattcaatt
    15181 tttgaatata gaaagatgga tatggcatgt aaaggcagga gccaacgcta cgcccgaggt
    15241 ggcatacagt ctatggcgtt tttctgacat aataggcaaa ttcgattttg cctccgcagg
    15301 agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct taatgagatg
    15361 ctttctcgcg ttaccccact gaccacccag gaaacgctgc cactggttca gtgttttggt
    15421 cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga taactccgct
    15481 atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct gcccgttgcc
    15541 ggtaaatcct ttgccggtca gccataccat ggtgaatggc ctgcgggtac ctgcattcgt
    15601 attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca ggagcagact
    15661 gaacaaacgg acaatggcgt gcgttttact gctgaagtgc gtagcgggca aaatattcgc
    15721 cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc cggcgggaac tcgcctgact
    15781 accgcagagc tgccagtaat tgcttcactg gggattgccg aagttccggt gattcgtaaa
    15841 gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca gccgctgggc
    15901 gacggccaaa tctacgacac caaccgtctc gccgtacacc tgatgttaga acagctgggt
    15961 tgcgaggtaa tcaacttagg gattatccgc gacgatccac atgccctgcg cgccgcattt
    16021 attgaagccg acagccaggc ggatgtagtg atcagttccg gcggtgtttc agtgggtgaa
    16081 gcggattaca ccaaaatgat tcttgaagag ctgggggaga tcgccttctg gaagctggcg
    16141 attaaaccag gtaaaccgtt cgcgttcggt aaactcagca atagctggtt ctgcggcctg
    16201 ccgggcaacc cggtttcagc gacgctgacc ttctatcaac tggtacagcc tttgctggca
    16261 aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc agcgcgtacg cacagcgtcc
    16321 cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg gcgtgctgca acgcaacgcc
    16381 gacggtgaac ttgaagtgac gaccaccgga catcagggtt cacatatatt tagctccttt
    16441 agtctcggca actgctttat cgtgctggaa cgcgatcgcg gcaatgtgga cgtgcgcgaa
    16501 tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg gaactcagcg
    16561 atcaggagat gctgcgctac aaccggcaaa tcattctgcg cggttttgat tttgacggtc
    16621 aggaggcgct aaaagattct cgtgtgctgg tagtcggcct cggtggactc ggctgcgcag
    16681 cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac ttcgacacgg
    16741 tttcgctctc gaatctgcaa cgccagacac tgcacagtga tgccacggtc gggcaaccga
    16801 aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg attacgccag
    16861 tcaatgcact gctagatgac gcagaacttg cagcattgat tgctgaacac gatctggtgc
    16921 tcgactgtac ggataacgtt gcggtacgta atcaactgaa cgcaggctgt tttgccgcga
    16981 aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg gtcttcactt
    17041 atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag aacgcgttaa
    17101 cctgcgtaga agcaggcgtg atggcaccgt tgataggcgt aattggttcg ttgcaggcaa
    17161 tggaagcgat caaactgctg gcaggttatg gcaaaccggc cagcgggaaa atcgtcatgt
    17221 acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg gggtgtgagg
    17281 tgtgcgggca gtaattgcca cctccaagcc tgataagacg cgacaagcgt cgcatcaggc
    17341 atttgctcca gacgccagat gcggcgtgaa cgccttatct ggcctacggt tcgggcacag
    17401 atttccaagc gcggcaggca tcggtgcact ccgccacgtc atcagcaatt tatgaggatg
    17461 cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc tgccagtcct gctcaaactt
    17521 cgtcacagcg gcttccaccg ccggatagct aatcatctgt tgtgccacat ccagtggcag
    17581 agtaattgat tcacatccgg ccagtaagca gtccagcgcc tgacgcgggg ttttgaaact
    17641 cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta agtcggtcac
    17701 agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg caacatattc
    17761 cgcacctgcc agcgccgaca gcagcccttg tgccgcgcca tataccgcgg ttcccagcgt
    17821 cggaatccct tccgctttta acatcttaat cgctgccagc ccctcggctg tcaccggaac
    17881 tttcaccacg atatccgcaa taatagaacg cagcttaagc gcgtcattca ccatcccttc
    17941 agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg cttcatgaag
    18001 ttgcggaagc acaacatcca gcggtttttt acccgcggcg ataatgcttg ggttagtggt
    18061 cacacccgcc agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt ctgaagtatc
    18121 cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta gactatcacg
    18181 ggaaaactct ctgaacagtt gacgcacatc aatctaactt tcattcgaaa gtattttaat
    18241 gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact cgacccatga
    18301 tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct gccgctggtg
    18361 tcagaacccg gaaagccgcg cccgtacgca ggatctgctg tatgacgcac gactgtgtct
    18421 ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg cgctgaatgg
    18481 tttgcttatt catcgggaaa agttaacccc ggagcatctg acggcgttaa ccgactgctg
    18541 tccgacacag gcgttaaccg tgtgtggtga agtgaaaagc gttgaggaga tcatggcgac
    18601 cgttctgcgc gataaaccgt tttacgatcg cagcggcggc ggcttaacgc tttcgggtgg
    18661 tgaacccttc atgcagccgg aaatggcgac ggcgctgctg caagcctgcc acgaggcagg
    18721 cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg ccccttctct
    18781 gccctatatc gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc cgtttaaaca
    18841 gtggaccgac ggtaacgccg ccagggtgct ggataacctg aaaaaactcg ccgcagcggg
    18901 caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg aaacctctgt
    18961 aaaagccatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc attttctgcc
    19021 ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg acgccccgga
    19081 aaaaccgctt gatgcgccag agctgctcga ttttgcccag cagtatgcct gccagaaagg
    19141 gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga cacgctcagc
    19201 gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaagccgcc agtctgtacc
    19261 gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc gatcccggta
    19321 cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca ccatctggat caaacacgat
    19381 gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt cccggaatat
    19441 actgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg tgctggcttt
    19501 gcggtgagcg aagagaacaa acgtgttctg catgaagtgt gcccgtggtg gcgcggtcag
    19561 acggtacagg atcgctgcta tggcatgttt accgatgagc aaaaaggtct gctggcgacc
    19621 ggcatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc ggtcaatttc
    19681 ccgctgctgc tggaaaaagg gcttgatggt ctgcgtgaga aagtagcgga acgtcgctcg
    19741 cgcatcaatc tgacggtgct ggaagatttg cacggcgaac agttcctgaa agcgattgat
    19801 atcatgctgg tggcagtcag tgaacacatt gaacgtttcg ctgccctggc gcgtgcaatg
    19861 gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc tggcgatggc agaaaactgc
    19921 gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact gtgttacttc
    19981 atccagttga ttttgcagat tgaatctaac ggtcactcgg tgtcgtttgg tcgtatggat
    20041 cagtatctct acccgtacta tcgccgcgac gttgaactga accagacgct ggatcgcgaa
    20101 cacgccatcg aattgctgca tagctgctgg ttgaaattgc tggaagtgaa caaaatccgc
    20161 tccggctccc actcaaaagc ctctgcggga agtccgctgt atcagaacgt caccattggc
    20221 ggacaaaatc tggttgatgg tcaaccaatg gacgcggtga atccactctc ttacgcgatc
    20281 ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg ttaccatgca
    20341 ggaatgagta acgatttcct cgacgcctgc gtacatgtga tccgctgcgg cttcgggatg
    20401 ccggcattca acaacgacga aatcgtgatc ccggaattta ttaaactcgg tattgaaccg
    20461 caggacgctt atgactacgc agcgattggt tgtatagaaa ccgccgtcgg tggcaaatgg
    20521 ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct ggcggcgctg
    20581 gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc cacaagaaaa agcgttgtcg
    20641 gcaggtaact tcaacaactt cgatgaagtg atggacgcgt gggatacgca aatccgttac
    20701 tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga agagaacgtg
    20761 cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa aagtatcaag
    20821 caaggcggcg cgaaatatga ctgggtttct ggcctgcagg tcggtattgc caacctcggc
    20881 aacagcctgg cggcagtgaa gaaactggtg tttgaacagg gcgcgattgg tcagcaacag
    20941 ctggctgccg cactggcgga tgacttcgat ggcctgactc atgagcaact gcgtcagcgg
    21001 ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ccgtcgatac gctgctggct
    21061 cgcgcttatc agacctatat cgacgaactg aaacagtacc ataacccacg ctacggtcgt
    21121 ggtccggttg gcggcaacta ttacgcgggt acgtcgtcta tctccgctaa cgtaccgttt
    21181 ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc acaccccgct ggcagaaggc
    21241 gctagtccgg cctctggtac tgaccatctc ggccctactg cggtcattgg ctcagtgggt
    21301 aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact gaatccggca
    21361 acgctggaga acgaatctga caagcagaaa ctgatgatcc tgctgcgtac cttctttgaa
    21421 gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct gctggaggcg
    21481 aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg tcgcgggtta ttccgccttc
    21541 ttcaccgcgc tctctccaga cgctcaggac gatatcatcg cccgtactga acatatgctg
    21601 taatctcttc ggcccggctg cggcgtcgcc gggctaaatc attcacatca tcaatttcat
    21661 ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta cactgttacg
    21721 gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca cagatatgga
    21781 cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg cgcaatatca
    21841 ggaactgaaa aagcgcggca ttaagttcgt tgtcgccagc ggtaatcagt attaccagct
    21901 tatttcgttc tttcctgagc taaaggatga gatctctttt gtcgcggaaa acggcgcact
    21961 ggtttacgaa catggcaagc aactgttcca cggcgaactg acccgacatg aatcgcggat
    22021 tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg gtctgcaaag
    22081 tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa aacactacca
    22141 tcgcctgaaa cctgtaaaag attatcagga gattgacgac gtactgttca agttttcgct
    22201 caacctgccg gatgaacaaa tcccgttagt gatcgacaaa ctacacatag cgctggatgg
    22261 cattatgaaa cccgtcacca gtggttttgg ctttatcgac ctgattattc ccggtctaca
    22321 taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc aaaatgtggt
    22381 agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt attcctttgc
    22441 gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg atgataataa
    22501 tcatgaaggc gcgctgaatg tgattcaggc tgtgctggat aacacatccc cttttaacag
    22561 ctgacctctc taccggagcc atgcgctccg gttttccctt ctttttacat cctgtacatc
    22621 acatcaaatc attaaacttg cattaactta tttataactt gtattgtcac atgcaaatta
    22681 gcatagtttc gaatgaaagt taatgcaagg tggttatggc ttctactttt accagcgaca
    22741 cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg cgggcgcagc
    22801 ttggcgatgt ccagcagatc tttaatcagc taagcgatga cattgccacg cgagtggctg
    22861 aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct tatgccgata
    22921 tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc ggttgtgcgg
    22981 tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg atgctggact
    23041 atctgaaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat ttcttcggga
    23101 cgctcagcgc ttcacgtccc gagatttacc ccatctactg gtcgcaggcg caaatgcagg
    23161 cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg tggacatttg
    23221 aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct gaccgtatcc
    23281 gccgccttcc gcccggaacg acctccaaag gtcttggagc gcataccgac tccggggcgc
    23341 tggaacgctg gctgcttcca gcgtatcagc acgttttcgc caacgtcttt aatggcaatc
    23401 tggcgaaata cgatccctgg catgcggcac atcgtacgga agttgaagag tacacggtgg
    23461 acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggactgcg ctctcggata
    23521 tgctgcctgg tcaggggttg ctgcacgtcg tgcccattcc tgaagccatg gcgtacgtac
    23581 tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta gcgcccggaa
    23641 gagtgttgcc ggtatcagag caatggcatc cactgttaat tgaggcgtta accagcattc
    23701 caaaactcga ggccggagac tccgtctggt ggcactgcga cgtcatccat tccgttgccc
    23761 ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat tcctgcggca ccgatgtgcg
    23821 agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc gcatcgccgg
    23881 gcgacttccc gcgcgaggac tatgaaacaa actgggaagg acgctttacg cttgccgacc
    23941 tcaacattca cggtaagcga gcgttgggca tggatgtttg agagttaatg gaagtgaatt
    24001 gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaagt gcgctttctt
    24061 aatgcctgat gcggcgttaa cgccttatcc ggcctacaaa gtcctgcaga ttcaatatat
    24121 tacaggtact gcgtaggcct gataagcgta gcgcatcagg ctattttgca tttgtcatca
    24181 gccgtcgtca ccccgacagt atcggggtga cggataatca cttactcgat ccctttacta
    24241 cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt gatttccaga
    24301 atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat cagcgtgccc
    24361 tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag gtggttggtc
    24421 ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa cagcatccgc
    24481 cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg gctgaacagc
    24541 aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt ccactgactc
    24601 atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg ggcgtagtaa
    24661 ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag atcgcccacc
    24721 agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc aagtttttca
    24781 ccgacctcca gcagcaggtt gagattttta aacagtggac cgttatcaaa ccctttggtc
    24841 agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa acggataaac
    24901 gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat ctggcgcgcg
    24961 cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac gaaagattgc
    25021 aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc acgcgcttgg
    25081 gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc gccgtaatcc
    25141 agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga gatgatgatc
    25201 atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc gatgtcgagg
    25261 ttgttggtcg gttcgtcgag caggagaata tccggctcag caaacagcgc ctgcgccaga
    25321 agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg ctgttccact
    25381 ggaattccca cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta accgtccatt
    25441 tcgccgtatt tcacttccag atcggccact ttatagccgt cttcttcact catttccggc
    25501 aaagcataga tgcggtcgcg ctcctgcttc acttcccaca actctttatg ccccatgatc
    25561 accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag cttaccaatg
    25621 cgctcgttgg gatcgaggga aacgttaccc agcgtcggct caaggtcgcc gccgagaatc
    25681 ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg gttgccgccg
    25741 ccgaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat ggtgacgtta
    25801 ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca ttatgccata
    25861 agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa ggcaaaaaat
    25921 gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat gcgcttccaa
    25981 tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta acatgaatat
    26041 gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca gtaccgcctc
    26101 tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga atcaggtgat
    26161 caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt accagatggg
    26221 gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga aaggcggtac
    26281 tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag gcatcgtcat
    26341 taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg ttatcgtgct
    26401 gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca ccaaagttga
    26461 gcataagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag agtaccgcgc
    26521 tgcgggcgaa ccgctaccgg ctgtcgttcc ggcaggtccg gataacccga tgggtctgta
    26581 tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact tcggtatcgg
    26641 cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat tcctgttcga
    26701 gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag cgaccaccga
    26761 gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg aagcccagtt
    26821 tgaaggtcag gaaatcgtgc caattaccct gacgaagagc gtgcagacag tgaccggtca
    26881 gccagatgtt gaccaggttg ttcttgatga agcgatcaaa aatcgttccg ggatgccggt
    26941 tcgcctgaat taatcgtcaa aacgtaaaac aaaggcggac tcataatccg ccttttttat
    27001 ttgccagacc ttaccaggcc gggatcataa ctaacaaaac gtatcccacc agcgccgccc
    27061 acaacagcat cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca
    27121 tacgtagcgc aatcaaattt gccagcgatc ctggtaataa accaaagccc cccacgttta
    27181 ccgcccatgc cagtaataaa gacggaggta cataattgag cagcaaaatg gtactcggca
    27241 cgttactgat cacctgcgat aaaccgactg ccgttaacca taacccgggt tcagataaat
    27301 gactcacgtt acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa tggacgtcga
    27361 taaacatcgc cataaacacc agcagcaacg tccagtccac actcaacacc acgcgacgcg
    27421 ccagcagcac aaagcccgcc gccacaatca ccagtcccca cagctcttgt ttgaactcca
    27481 gcgccgtcag aaagacgata tacagcccca gacaattcca caccagccgc ggtttccact
    27541 ccggtgtttg cacccccgta tggtattgca gcgcctttcc agggaaacaa caccagcaca
    27601 acagcaggag tgtcagcatc attgcgccag ccatcggtgc catttgggca ataaatccgg
    27661 caaacgaaag accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca
    27721 gtagcgaacc agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct
    27781 cacacagtct ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg
    27841 tcagaaaggt agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg
    27901 tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac
    27961 ctttggtcag cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag
    28021 gccaggattt cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca agaattaata
    28081 actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg
    28141 tttctgggta aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc
    28201 ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac
    28261 tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac
    28321 gccacgccag gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc
    28381 caccgtcggt tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac
    28441 ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc
    28501 ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt
    28561 ttttgctatt ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata
    28621 catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta
    28681 acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc
    28741 cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta
    28801 tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg
    28861 atatggtcaa caacaaaccg cagcaagata aaccttcaga taaatagcgg cgtcgcggta
    28921 cgccgcttca ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaaaccactt
    28981 tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg
    29041 cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt
    29101 tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt
    29161 ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg
    29221 gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta
    29281 ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg
    29341 cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt
    29401 tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac
    29461 tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg
    29521 gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa
    29581 aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt
    29641 gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat
    29701 ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa
    29761 gaacggatgg caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg
    29821 aagtatcaga cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc
    29881 tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct
    29941 gactacattg ctgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta
    30001 gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc
    30061 cagacctggt ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc
    30121 atcgccaaac gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa
    30181 aacacgaaag acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg
    30241 caaccgcagc tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca
    30301 caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg
    30361 acgcaaacgg acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc
    30421 ttctctctgg tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg
    30481 caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt
    30541 tccagcggcg ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta
    30601 ggctttttct cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt
    30661 atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat
    30721 tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg
    30781 agaggcggat ttttatatca ccaaagtgat tagaagcggt aaccaacacc ggcaatccag
    30841 gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt
    30901 tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt
    30961 ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag
    31021 atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt
    31081 ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc
    31141 acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg
    31201 ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct
    31261 acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt
    31321 ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta
    31381 atgggaaact cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc
    31441 catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg
    31501 catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa
    31561 tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt
    31621 gggtatatcc agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg
    31681 cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg
    31741 gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt gagcgcaccg
    31801 ggtgtcactg cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca
    31861 tggcgactgc gctttgccaa agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg
    31921 ggtgggatga attatctttt ttatctttct attcagacag taccgctggg tattgcggtg
    31981 gcgctggagt tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat
    32041 ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac
    32101 gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct
    32161 atttacattt taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtagcaatt
    32221 ggttcgttga ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca
    32281 ctctggcact ggtcggttat tccattgggt ctggctgtcg ttattctctc gaccgctctg
    32341 ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg
    32401 atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg
    32461 acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg gtctacgctg
    32521 acagtacgca aagagagcaa aataaaagaa ttagacatta attaaattta catttctgta
    32581 tggttatgca taaccatgca gaatttctcg ctacttttcc tctacaccgt atttatatat
    32641 cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcagataa ataagaattg
    32701 ttcttatcaa tatatctaac tcattgaatc tttattagtt ttgtttttca tgcttgttac
    32761 cactattagt gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag
    32821 tctcgttaat tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt
    32881 agttaaatca aaagcgacca atctgcttta tacccgcaac gatgtctccg acagcgagaa
    32941 aaaagcaaca gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat
    33001 taccaaacaa gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct
    33061 ggatggcttc cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca
    33121 gctgggcggt gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag
    33181 ttacccgctg gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc
    33241 aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga
    33301 tatcctgacc gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat
    33361 cgaataaatc catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac
    33421 aggaggccgt tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta
    33481 tccccagctg attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga
    33541 ttgttttttc atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc
    33601 accagattgg tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca
    33661 cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaat tggcacgatt ttttcatata
    33721 tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg
    33781 tattaaaagt ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg
    33841 ataaaaaatt agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg
    33901 ataaatatgt gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg
    33961 attacgaact gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg
    34021 tcgatctggc gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct
    34081 ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga
    34141 aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg
    34201 attacgcgaa agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg
    34261 cctatatgga actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc
    34321 tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag
    34381 cgcagcaata cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg
    34441 gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttca
    34501 gtactgaacc gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc
    34561 ctggggagag gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa
    34621 tttgattatt tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct
    34681 ggcctgccat tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg
    34741 gtctggctgg cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt
    34801 ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg
    34861 tccaggtgat gtttatctat ttcgccctgc cgatggcgtt taacgactta cgcatcgacc
    34921 catttactgc ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca
    34981 cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc
    35041 tttcacgttg ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc
    35101 cgccgctggg taaccagtgg atcatcagca ttaaagacac ctcgctgttt attgttatcg
    35161 gcgtggcgga actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg
    35221 agatctggag cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc
    35281 tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca
    35341 ctttggccca acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt
    35401 ggtgattatc gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact
    35461 ggaagaaatc acctccggcg atctgattgt cgatggcctg aaggtcaacg atccgaaagt
    35521 tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc
    35581 gcatctgacg gcgctgggaa acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa
    35641 agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc
    35701 acatcactac ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc
    35761 gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga
    35821 actgcgccat gaagtgctga aggttatgca ggatctggct gaagaaggga tgacgatggt
    35881 gatcgtgacc cacgaaatcg gttttgccga gaaagtagct tcgcggctga tctttatcga
    35941 caaaggccgg attgcggaag atggcaatcc gcaggtgttg atcaagaacc cgccgagcca
    36001 gcgcttgcag gaatttttgc agcacgtctc ttaataagac acattgcctg atgcgctacg
    36061 cttatcaggc ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga
    36121 taaggcgttc acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac ctgcttaata
    36181 tcccttcctc cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc
    36241 cctcccccag catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg
    36301 tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc
    36361 ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga accggatatc
    36421 gaacaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta
    36481 aagagttgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga
    36541 agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca
    36601 gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg
    36661 gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa
    36721 tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctat
    36781 atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc
    36841 ttccggcgat gattattggg gcgtttatta tcgacctgct gttactggcc ctgacattat
    36901 ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc gctttccaac
    36961 aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga
    37021 ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt
    37081 actggggtcg tcgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggccg
    37141 tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta
    37201 tgctgtgcat gaccgtctgg gcgttgtacc tgatcttccg aaataaaaaa gagattaccc
    37261 agcatatgct caactttgcg gagcattcgc tggctttttt cagcctgttt atccgcgcct
    37321 ttgcgctggt gtggcactgg ctggcaagcg cctattttat catgctgttt ttcttttcgt
    37381 tgttcgatcc gggcaacagt ctgaaattta tgatgggtgc aacggtgcgc agcctggcga
    37441 ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggcg aaaaccatca
    37501 ccctctcgcc acatactcag cgtaactatc cggagctaca aaaacggctg aatggctggc
    37561 tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta atgttgctgt
    37621 tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa
    37681 ccgtagatat cctgatccgc atcgcactca ttcttttctt ctcggcggtt ggctggacgg
    37741 tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca
    37801 gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta
    37861 ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag
    37921 gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggta aaagatatta
    37981 tcaccggggt atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg
    38041 ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata
    38101 ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg
    38161 gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca
    38221 atcaggcact gaaagatgcg gtaacggaat tgatggaaaa cgaagaaatt cgcgggctga
    38281 ttattggtga accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc
    38341 gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc
    38401 aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc
    38461 tgcctgctcc gggcgcgacc ccggctgaac cgctgccgcc gggggagcca acgctttaac
    38521 gttggcgatt aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc
    38581 gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg
    38641 tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca
    38701 tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag ttgacttcac
    38761 cgccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca
    38821 gccccaggtt acggcgttta cgctcactac ctgcccgcgc cgcagcggcg gaatcgtgga
    38881 atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt gtggatgatg ccgttaaaaa
    38941 tcgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac
    39001 tgataatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc
    39061 agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca
    39121 gaatgctggc attcgctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat
    39181 gaatataatc cgcccggccc ggtactggtg ggcagagaaa accgtcgggg atatcccagt
    39241 tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat
    39301 tggcaaagtc tacgctttgc tcccctgcgg gtgtaagcgt gaggaattgc ctgagttcag
    39361 gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca
    39421 accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat
    39481 aacgcgacag ggtaaatatc tggctgcgtt tatcattccc caaaacctta acatggctat
    39541 ggtttatggc aattcatcat aaaaatcagc attatgcatg tcattgattt gtcttatttt
    39601 gtaaaaatat atcgttcaaa aaataagcat ttctctgtca attttttgcg tcatttgcat
    39661 cgcgcaggcg acctacactt atcaggcact acccgttgta tttatgagga tgtcattatg
    39721 aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat
    39781 gccgcgcaac cgatgagtaa cctggacagc ggtcagctac ggcccgccgg taccgtttcg
    39841 gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa
    39901 caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat gttaggtact
    39961 gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg
    40021 cctgttttgt aatcaaaaaa catcaaatat gaacgcaatg taaataaatg tatttctttt
    40081 tcgcgcaatg ggtgatagaa aatcgcatca agtgataatg cttatcaaaa ttattatcac
    40141 ttgcacgaac actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt
//
LOCUS       DAJTRN010000027        39990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRN010000027 DAJTRN010000000
VERSION     DAJTRN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..155
                     /locus_tag="OO328_001467"
     CDS             <1..155
                     /locus_tag="OO328_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5930078.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO328_001468"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO328_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5930079.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO328_001469"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO328_001469"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5930080.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO328_001470"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO328_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO328_001471"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO328_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5930081.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO328_001472"
     CDS             complement(5626..7134)
                     /locus_tag="OO328_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5930082.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO328_001473"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO328_001473"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5930083.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO328_001474"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO328_001474"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5930084.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO328_001475"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO328_001475"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5930085.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO328_001476"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO328_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5930086.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO328_001477"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO328_001477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5930087.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO328_001478"
     CDS             complement(14283..15602)
                     /locus_tag="OO328_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5930088.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO328_001479"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO328_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5930089.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO328_001480"
     CDS             16699..17034
                     /locus_tag="OO328_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5930090.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO328_001481"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO328_001481"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5930091.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO328_001482"
     CDS             complement(17790..19172)
                     /locus_tag="OO328_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5930092.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO328_001483"
     CDS             complement(19358..20302)
                     /locus_tag="OO328_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5930093.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO328_001484"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO328_001484"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5930094.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO328_001485"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO328_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5930095.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO328_001486"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO328_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5930096.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO328_001487"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO328_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5930097.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO328_001488"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO328_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5930098.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO328_001489"
     CDS             complement(25947..26768)
                     /locus_tag="OO328_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5930099.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO328_001490"
     CDS             complement(26868..26951)
                     /locus_tag="OO328_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930100.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO328_001491"
     CDS             complement(27044..27379)
                     /locus_tag="OO328_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5930101.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO328_001492"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO328_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30047
                     /locus_tag="OO328_001493"
     CDS             29136..30047
                     /locus_tag="OO328_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5930102.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30182..31402
                     /gene="mlc"
                     /locus_tag="OO328_001494"
                     /gene_synonym="dgsA"
     CDS             30182..31402
                     /gene="mlc"
                     /locus_tag="OO328_001494"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5930103.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31527..32222
                     /gene="bioD"
                     /locus_tag="OO328_001495"
     CDS             31527..32222
                     /gene="bioD"
                     /locus_tag="OO328_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5930104.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO328_001496"
     CDS             complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO328_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5930105.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO328_001497"
     CDS             complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO328_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5930106.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34283..35137)
                     /locus_tag="OO328_001498"
     CDS             complement(34283..35137)
                     /locus_tag="OO328_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5930107.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO328_001499"
     CDS             complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO328_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5930108.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35767..37805)
                     /locus_tag="OO328_001500"
                     /pseudo
     CDS             complement(35767..37805)
                     /locus_tag="OO328_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37866..38975)
                     /locus_tag="OO328_001501"
     CDS             complement(37866..38975)
                     /locus_tag="OO328_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5930109.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38958..>39990
                     /locus_tag="OO328_001502"
     CDS             38958..>39990
                     /locus_tag="OO328_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5930110.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10164 a         9700 c        10242 g         9884 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
    29761 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
    29821 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
    29881 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
    29941 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
    30001 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
    30061 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
    30121 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
    30181 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
    30241 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
    30301 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
    30361 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
    30421 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
    30481 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
    30541 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
    30601 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
    30661 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
    30721 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
    30781 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
    30841 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
    30901 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
    30961 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
    31021 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
    31081 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
    31141 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
    31201 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
    31261 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
    31321 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
    31381 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
    31441 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
    31501 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
    31561 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
    31621 aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
    31681 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
    31741 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
    31801 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
    31861 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
    31921 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
    31981 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
    32041 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
    32101 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
    32161 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
    32221 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
    32281 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
    32341 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
    32401 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
    32461 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
    32521 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
    32581 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
    32641 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
    32701 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
    32761 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
    32821 tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg
    32881 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
    32941 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
    33001 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
    33061 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
    33121 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
    33181 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
    33241 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct
    33301 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
    33361 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
    33421 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
    33481 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
    33541 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc
    33601 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
    33661 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
    33721 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
    33781 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    33841 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
    33901 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    33961 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    34021 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
    34081 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
    34141 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    34201 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    34261 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
    34321 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
    34381 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    34441 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    34501 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
    34561 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    34621 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    34681 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
    34741 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
    34801 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
    34861 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
    34921 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    34981 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
    35041 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    35101 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    35161 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    35221 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    35281 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
    35341 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    35401 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    35461 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
    35521 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
    35581 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
    35641 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    35701 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    35761 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    35821 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
    35881 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
    35941 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
    36001 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
    36061 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg
    36121 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    36181 gcgaggatca catagcccat attccctgca gattcatgag agataaggtc ttcttgctct
    36241 gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg
    36301 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    36361 atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    36421 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg
    36481 tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc
    36541 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    36601 atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg
    36661 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    36721 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    36781 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    36841 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    36901 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca
    36961 tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag
    37021 cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa
    37081 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    37141 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    37201 gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag
    37261 ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg
    37321 tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg
    37381 atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg
    37441 acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc
    37501 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    37561 tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag
    37621 ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc
    37681 atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag
    37741 gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    37801 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    37861 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    37921 ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca
    37981 gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt
    38041 ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg
    38101 ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag
    38161 gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    38221 ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag
    38281 gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    38341 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    38401 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta
    38461 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    38521 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    38581 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg
    38641 aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg
    38701 ttaggaataa tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat
    38761 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    38821 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg
    38881 tagttccaga tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg
    38941 ctacttaagg tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt
    39001 ctttctctgg gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc
    39061 tgacacaaat caatctactc agcgtagcta agcctcagtt agtccgcaga gccaatggtc
    39121 gatatactat ccgcttcaga ctcaagggac agaccacacc gtttctttca gtatcgaccc
    39181 gcagcacaga caggagagta gccacaatgc gtcagagaga gcttgcagcc accgctaagg
    39241 ccttcatgtt ggataggcct gaagtctcac tacaggagct tacagagcat ctccggtcta
    39301 tggctgagca gttcctgaca gacgctagtg acgattactg gaatggactt gaggtcgcta
    39361 ctctggtcga cgagaagtcc aacctgaagg agctagccgc tacgcaagcc ttgagtctgg
    39421 accagcagaa gggcataagg ttagcgctgg aagtcctgac cgcagcacag cagagagtcg
    39481 acactggtga tacctctggt ctgattaagc tgattgatga taataacctt actgatgact
    39541 caactatagg cgactctacg tcaattctga ataatgagca gggggataga ccggcagttt
    39601 tcacacagga gcgccagtca tctgtagttt tttcaagcct agtgtcctcg ctgctggctg
    39661 agaaggtcca gaccctgaag acatccagct acaaggacct ctcgtcctca ctcaatactg
    39721 tctcccggtt cctgcctgaa gacatggacc tgatgtcccg ctctgggtgg ctggctgtca
    39781 gggactctat gctggcttct gaggtgagac catcgacaat caacaagctg ctgaccaaag
    39841 cgaagatgtg tctcgattat ggcctgatga atgggcaact tgagggccgc aaccctattg
    39901 aacggatgaa gctgaccaag gatatcgact ccaagcgcag agcgttcaca gacgaggagc
    39961 tggagagact tctggtccgt gttgagtctg
//
LOCUS       DAJTRN010000028        38006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRN010000028 DAJTRN010000000
VERSION     DAJTRN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..338)
                     /locus_tag="OO328_001503"
     CDS             complement(<1..338)
                     /locus_tag="OO328_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5930111.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGR"
     gene            complement(339..1271)
                     /gene="thrB"
                     /locus_tag="OO328_001504"
     CDS             complement(339..1271)
                     /gene="thrB"
                     /locus_tag="OO328_001504"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5930112.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(1273..3735)
                     /gene="thrA"
                     /locus_tag="OO328_001505"
     CDS             complement(1273..3735)
                     /gene="thrA"
                     /locus_tag="OO328_001505"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5930113.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(3761..3880)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(3816..3881)
                     /gene="thrL"
                     /locus_tag="OO328_001506"
     CDS             complement(3816..3881)
                     /gene="thrL"
                     /locus_tag="OO328_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5930114.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(4095..4781)
                     /locus_tag="OO328_001507"
     CDS             complement(4095..4781)
                     /locus_tag="OO328_001507"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5930115.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(5181..5321)
                     /locus_tag="OO328_001508"
     CDS             complement(5181..5321)
                     /locus_tag="OO328_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930116.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            5417..6133
                     /gene="arcA"
                     /locus_tag="OO328_001509"
     CDS             5417..6133
                     /gene="arcA"
                     /locus_tag="OO328_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5930117.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(6192..7544)
                     /gene="creD"
                     /locus_tag="OO328_001510"
     CDS             complement(6192..7544)
                     /gene="creD"
                     /locus_tag="OO328_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5930118.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(7602..9026)
                     /gene="creC"
                     /locus_tag="OO328_001511"
     CDS             complement(7602..9026)
                     /gene="creC"
                     /locus_tag="OO328_001511"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5930119.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(9026..9715)
                     /gene="creB"
                     /locus_tag="OO328_001512"
     CDS             complement(9026..9715)
                     /gene="creB"
                     /locus_tag="OO328_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5930120.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(9728..10201)
                     /gene="creA"
                     /locus_tag="OO328_001513"
     CDS             complement(9728..10201)
                     /gene="creA"
                     /locus_tag="OO328_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5930121.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            10412..11281
                     /gene="robA"
                     /locus_tag="OO328_001514"
     CDS             10412..11281
                     /gene="robA"
                     /locus_tag="OO328_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5930122.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(11278..11925)
                     /gene="gpmB"
                     /locus_tag="OO328_001515"
     CDS             complement(11278..11925)
                     /gene="gpmB"
                     /locus_tag="OO328_001515"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5930123.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            11977..12497
                     /gene="yjjX"
                     /locus_tag="OO328_001516"
                     /pseudo
     CDS             11977..12497
                     /gene="yjjX"
                     /locus_tag="OO328_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(12582..12908)
                     /gene="trpR"
                     /locus_tag="OO328_001517"
     CDS             complement(12582..12908)
                     /gene="trpR"
                     /locus_tag="OO328_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5930124.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(12998..14935)
                     /gene="sltY"
                     /locus_tag="OO328_001518"
     CDS             complement(12998..14935)
                     /gene="sltY"
                     /locus_tag="OO328_001518"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5930125.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            15146..16813
                     /gene="ettA"
                     /locus_tag="OO328_001519"
     CDS             15146..16813
                     /gene="ettA"
                     /locus_tag="OO328_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5930126.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEPPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(16920..17087)
                     /locus_tag="OO328_001520"
     CDS             complement(16920..17087)
                     /locus_tag="OO328_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930127.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(17121..18353)
                     /gene="nadR"
                     /locus_tag="OO328_001521"
     CDS             complement(17121..18353)
                     /gene="nadR"
                     /locus_tag="OO328_001521"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5930128.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(18374..19756)
                     /gene="radA"
                     /locus_tag="OO328_001522"
     CDS             complement(18374..19756)
                     /gene="radA"
                     /locus_tag="OO328_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5930129.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(19805..20773)
                     /gene="serB"
                     /locus_tag="OO328_001523"
     CDS             complement(19805..20773)
                     /gene="serB"
                     /locus_tag="OO328_001523"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5930130.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            20879..21523
                     /locus_tag="OO328_001524"
     CDS             20879..21523
                     /locus_tag="OO328_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5930131.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            21551..22566
                     /gene="lplA"
                     /locus_tag="OO328_001525"
                     /pseudo
     CDS             21551..22566
                     /gene="lplA"
                     /locus_tag="OO328_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            22598..22861
                     /locus_tag="OO328_001526"
     CDS             22598..22861
                     /locus_tag="OO328_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5930132.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(23022..23741)
                     /gene="deoD"
                     /locus_tag="OO328_001527"
     CDS             complement(23022..23741)
                     /gene="deoD"
                     /locus_tag="OO328_001527"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5930133.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(23798..25021)
                     /gene="deoB"
                     /locus_tag="OO328_001528"
     CDS             complement(23798..25021)
                     /gene="deoB"
                     /locus_tag="OO328_001528"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5930134.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(25073..26395)
                     /gene="deoA"
                     /locus_tag="OO328_001529"
     CDS             complement(25073..26395)
                     /gene="deoA"
                     /locus_tag="OO328_001529"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5930135.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(26522..27301)
                     /gene="deoC"
                     /locus_tag="OO328_001530"
     CDS             complement(26522..27301)
                     /gene="deoC"
                     /locus_tag="OO328_001530"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5930136.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            27559..29100
                     /locus_tag="OO328_001531"
     CDS             27559..29100
                     /locus_tag="OO328_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5930137.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            29072..29935
                     /locus_tag="OO328_001532"
     CDS             29072..29935
                     /locus_tag="OO328_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5930138.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(30149..30931)
                     /locus_tag="OO328_001533"
     CDS             complement(30149..30931)
                     /locus_tag="OO328_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5930139.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(30928..32001)
                     /locus_tag="OO328_001534"
     CDS             complement(30928..32001)
                     /locus_tag="OO328_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5930140.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(32123..32284)
                     /locus_tag="OO328_001535"
     CDS             complement(32123..32284)
                     /locus_tag="OO328_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5930141.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(32401..33006)
                     /gene="osmY"
                     /locus_tag="OO328_001536"
     CDS             complement(32401..33006)
                     /gene="osmY"
                     /locus_tag="OO328_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5930142.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(33399..34988)
                     /gene="prfC"
                     /locus_tag="OO328_001537"
     CDS             complement(33399..34988)
                     /gene="prfC"
                     /locus_tag="OO328_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5930143.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(35079..35756)
                     /gene="yjjG"
                     /locus_tag="OO328_001538"
     CDS             complement(35079..35756)
                     /gene="yjjG"
                     /locus_tag="OO328_001538"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5930144.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(35771..36217)
                     /gene="rimI"
                     /locus_tag="OO328_001539"
     CDS             complement(35771..36217)
                     /gene="rimI"
                     /locus_tag="OO328_001539"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5930145.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(36186..36599)
                     /gene="holD"
                     /locus_tag="OO328_001540"
     CDS             complement(36186..36599)
                     /gene="holD"
                     /locus_tag="OO328_001540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5930146.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            36702..37733
                     /gene="rsmC"
                     /locus_tag="OO328_001541"
     CDS             36702..37733
                     /gene="rsmC"
                     /locus_tag="OO328_001541"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5930147.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         9007 a        10467 c         9619 g         8913 t
ORIGIN      
        1 aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg
       61 acatcgcttt caacattggc gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct
      121 tccaggattt cctgtgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg
      181 gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtcg
      241 tgcggaaaaa acagcccctg atttttgccc aacccctggg ttacggcttg cgcaaagctg
      301 acctgctcgt tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg
      361 cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag gtagttctta
      421 cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacatagagc gaacaaggtc
      481 gggccggagc cggagatacc gctcgctacc gcgccgattt ccgcgaccgc ctgccgcgcc
      541 tgccggaagc caggcagtaa ccgttcacgg tagggttcag cgataacatc tttcatcagc
      601 ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa tgaagccagc cagatgtcgc
      661 ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagcccg ggcttctgcc
      721 gtcgagactt taattcccgg atacgccagc acccacagcc actcatcaaa ccctggcact
      781 tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccaag aaaacacggt
      841 gccacgttgt cgtaatgaat gctgccggag atacgtcctt ccagctcgcc catcaaagcc
      901 agcaaacgag tgtcattaag cggcttgccg cagtgttcat tcatcgccat cagcgccgcg
      961 accaccgaac aggcgctgga gcctaagccc gaaccgatcg gcatattctt ttccagagtc
     1021 atcgccactg gaatttgctt gccaagctcc tggcaaaaac gctcccagca ctgataaacg
     1081 atattttccc gtggttctga cggcagctta tcggcaaagc gtccgagttt gttgagactg
     1141 aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacgggtgcc
     1201 accgccgccc cgagcacatc aaacccgacg ctcatattgg cactggaagc cggggcataa
     1261 actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac
     1321 accggcagcg gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg gcagcggctg
     1381 ataatagtgg ctataaaagg ccagggcgtt ttcgccattt ttcactttga acagcggatc
     1441 attcccatcc acttcggcaa tcttcacgcg gcagacacca tcttcatcaa tattgccaac
     1501 atagcgcaaa acttttcctt catcacgggc cttcgccacg cgcgcggcaa agagatcgtc
     1561 gagctgtgac agattcgcca taaaagcggc aacatcaccc tcggcgttaa actctgcggg
     1621 cagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag
     1681 aatcaatagt ttacgcgcca catccatacc agaaagatca tctcgcgggt ccggttcggt
     1741 ataacccatt tcccgcgcca gcgtggtcgc ctcggagaaa ctcatgcctt cgtctaactt
     1801 gccgaagata taagaaagcg aacctgaaag aatgccggag aacttcacca attcatcacc
     1861 tgcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc
     1921 atagaggaat ttacgccgcg atttttccgc cgcataacgc aactgatgat agtaatccat
     1981 cgacgaggtg ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc gcaggaagtc
     2041 ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg ggttcagcag
     2101 atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgtgc
     2161 aagttcttcc tgccagtttt ccagattaag gccatgcaca ctagtgagca gagccttcga
     2221 gttggcaacg ccgcagacac gtaagtcgat atgtttattc ttcagccagc tttgctgacg
     2281 cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat
     2341 aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc
     2401 gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt
     2461 gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca aggtgcgcat
     2521 accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg gctccagtaa
     2581 gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagctc gcacacagtc
     2641 gctttgtgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac
     2701 ggaaatacgg gcgcgtgaca ttgcagcaaa gacgcgcgcc gccatgccaa ccatcccttt
     2761 catccccggg ccggaaacgc tgaacattgc catgttattc agattggaaa tgcccttgac
     2821 cggtaattcg tcttcatcac ggctggcacc aatgagcgta cctggtgctt gaggatttcc
     2881 ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg
     2941 aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca tcgacttcaa
     3001 caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca
     3061 aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt agtcggaacc
     3121 gttacgtcca agcaccacca gttcgccttt ttcattaccg gcggtgaaac ctgccatcaa
     3181 caccatgtga tcagccggaa tgcggcttgc cgcaatacgg cgggtggact cagcaatatc
     3241 gacggtagat tcgaggtaat gccccactgc cagcagtttt tcgaccggat cgataacggt
     3301 aacgttgtga ccacgcgctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc
     3361 acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac taatgccatg
     3421 caggacatgt tttatttggg caaattcctg atcgacgaaa gttttcaatt gcgccagcgg
     3481 gaaccccggc tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac gttcggcatc
     3541 gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg ccaccaggtg
     3601 gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct
     3661 ttccagaata tcggcaacac gcagaaaacg ttctgcattt gccactgatg taccgccgaa
     3721 cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaaag cccgcactgt
     3781 caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta
     3841 atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta
     3901 atttttatct gtttgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa
     3961 taaaatttta atttactcac ggcaggtaac cagttcagaa gctgctatca gacactcttt
     4021 ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact
     4081 tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac
     4141 attgccgtgt ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc gaccagtttt
     4201 atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc
     4261 agttgatgtt ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat
     4321 tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc
     4381 ggcacaccag taagcacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg
     4441 cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcggcacc
     4501 agttctactg gcgtggcgta gtaatgatat ttcgcccgac tacgcgcagt ggtggcgaca
     4561 gtgaaatcga catcgtgtaa cgattcagcc agtgtcggga aaactttaat attatcaata
     4621 atatcaccag atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg
     4681 acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt
     4741 tctgctctgg cgggtgcgac caggataatc gttatacgca tattgccact cttcttgacc
     4801 aaataaccgc gaacagggtg atcactgtca acttattacg cggtgcgaat ttacaaattc
     4861 ttaacgtaag ccgcagaaaa agccctttac ttagcttaaa aaaggctaaa ctattttctg
     4921 actgcactaa cggtttaatt gttaaaaaat gctacatatc cttctgttta cttaggataa
     4981 ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa
     5041 ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca
     5101 aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt
     5161 gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt
     5221 gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg
     5281 atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa
     5341 tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc
     5401 aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac
     5461 acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga
     5521 tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat
     5581 caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt
     5641 tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat
     5701 cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg
     5761 caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga
     5821 aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga
     5881 tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa
     5941 cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa
     6001 accgcacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac
     6061 gccggatacg ccggaaatca tcgccaccat tcacggtgaa ggttatcgct tctgcggtga
     6121 tctggaagat taatcggctt taccaccgtc aaaaaaacgg cgctttttag cgccgttttt
     6181 atttttcaac cttatttcca gatacgtaat tcatcgtccg ttgtaacttc tttactggct
     6241 ttcattttcg gcagtgaaaa cgcataccag tctatattac gagtcacaaa catcatgccg
     6301 gccagcgcca ccaccagcac actggttccc aacaacagcg cgctatcggc agagttgagc
     6361 agtccccaca tcactccatc cagcaacaac agcgcgaggg taaacaacat gctattgcgc
     6421 cagcctttca ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg
     6481 attatccatg ccacggtaaa accgatatgt tcagaaagcg ccagcaagag caaataaaac
     6541 atcaccaatg aaagccccac cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc
     6601 gtttcaaaaa caaagaacgc cataaaagtc agtgcaatca gcagaatggc gtacttagtc
     6661 gcccggtccg ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgggg
     6721 aagttttccc agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac
     6781 cagctgcttt gccattgcgc ctgaaaacct gactcgctag cttcccgttt ggctggtaga
     6841 aaatcaccta aaaaactggg atgcggccag ttgctggtta aggtcatttc gctattacgc
     6901 ccggcaggca ccacagaaag atcgccggta ccgcttaaat tcagtgccat attcagctgc
     6961 aggttctgct tccgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc
     7021 tctaacccgg taccgggttc aatggtcagt gccgttccgt taacttcagg cgctttcacc
     7081 acaccaatac cacgcgcatc cccgacgcta atcacaataa agggcttgcc taagatgata
     7141 tttggcgcgt tgagctcgct aagacgcgaa acatcgaaat cggcttttag cgttaaatca
     7201 ctgtgccaga cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca
     7261 tcaaccatca atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt
     7321 ttatcctctt cctgcaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaacg
     7381 agtttttgcg gcccgctggt actttggcga atcgcatctt ccacatcgct acggtaatcg
     7441 gcacgttcga caatcacttg ccgaatcagc attatcggaa tcaacaacag caatactgca
     7501 ccaaacaagg tggtcatttt ccagaacagg ggggatttca acatagtctt ctcctttgca
     7561 atggcagcag gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga
     7621 agtcgtagcg aggccagcac gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta
     7681 aacaaacggg cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt
     7741 gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca
     7801 ctaccggtat ccagcacctt aagcgtgacg tgttcctgat ccacttcggc gcttagcgtt
     7861 atgcaaccgc tctcgggagt aaaatcgatg gcgttatcca gtaaattccc cagcgcctga
     7921 tccagtaacg ccggttcagc agcaacatta acctcggtgg gcataacatg caaagtgatg
     7981 tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa tgccgccaca
     8041 tcaacagcag tcagaacgac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt
     8101 tccaccagcg cctgcattcg cgcattttgt gtcagaatgt tgtcagtaaa acgagccact
     8161 acttcaggcg gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg
     8221 ctttttagct catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc
     8281 ttcacgcgca tactttccag cgcctgcgcg agtttacgca gctcgctact accgagatcg
     8341 ggaagaggaa cgggcttatt gtcagtgacg gaatcagcat agcgagtgag ccgggcaatg
     8401 gagcggttga tccaccaaac catgcctgcg ccaatcacca gtgcaatccc caacaaaatg
     8461 gcgctggccc ataaaattcg ccgctcgctg cgtttaatga ccggagccat cgccgcgttc
     8521 ggtttgccta cgctcaaaac gccaataagc cgcgagccgc ccataatcgg cgcggcaaca
     8581 tacatcaccg aactttcggg atcggcagga ttttgcaacg tgctgcgcgc accatactga
     8641 ccacgcaacg ttagccagac gtcattccag cgcgaataat cctgtccaac ggctttattt
     8701 gccgaatcga acaatacttt gccatgcgca tcggtcatat agacatgata ttcattgcgc
     8761 actttgttaa tgccaccaat attggcgcga aacgggcgat gttgtagctg attaaacgcc
     8821 tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgca
     8881 agtaacgttg ccgtgtcgat caacgtcccc tccgttgctc ttcgcacgcc cggtttaact
     8941 tctttgacaa aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc
     9001 agcagcaacc gcatgccgat acgcattaca agcccctcag gctatatccc atgccgcgat
     9061 gagtattaat cggtgaaaga tcggggttga tggcgcgcag cttggcacgc agcgttttaa
     9121 tgtggatatc gacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatta
     9181 gttgctggcg ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact
     9241 cataccgcgt cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca
     9301 attcaaaatg accaatacgg atgacgggag acggcgtcga gaacttcttc acccgacgca
     9361 gtaaggtgcg caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt
     9421 cagcgccaat ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca
     9481 gtacaggtaa cgccggatag agcgccagta actggcggca caattcaaag ccactgatat
     9541 caggcaaacc aacgtcgaga atcatgacgt cgggaacctg ctggcgagct ttatccagca
     9601 ccggcaaacc tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca
     9661 gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgcataaatc
     9721 gcctctgtta ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac
     9781 cttctacaac tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcgtcataaa
     9841 agcgcacgac ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc
     9901 cctgagcttt gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag
     9961 aaatggccgc atcggaggta tcttctgcca ggcccaatcc ccctttaata ccaccggttt
    10021 tcgcccggct cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa
    10081 caattttgtg atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg
    10141 catgagccaa tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca
    10201 tattgttacc atttttatct attacacgag caattagttc gtcacggtaa ataacatatt
    10261 gattaagaac aaaatctcaa ttcttttatt tccgtcaagc cctaaaacat actctactaa
    10321 gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag
    10381 gtgctcgttg ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct
    10441 tttaatctgg ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa
    10501 agcaggttat tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat
    10561 tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc
    10621 gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg
    10681 cgcattcaag aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag
    10741 cgcctttggt attcgcccgc cgctacgtct gggtgaattc acaatgccag agcacaaatt
    10801 tgtcaccctg gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga
    10861 gcaaatctct gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa
    10921 cgcgccgacc attccgccgg tgctctacgg cctgaatgaa acgcgtccga gtcaggataa
    10981 agacgacgaa caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta
    11041 tgtactgacg gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga
    11101 aggtctggga accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat
    11161 gctcaatctg acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc
    11221 caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta
    11281 acgctgcagc tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac
    11341 gacccagcca gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt
    11401 acgcagacgt aagcggcgct ctgcccatgc tggtaatccg agaatcgtac tcaccagaca
    11461 tcccagtgca ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca
    11521 agattccagt gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat
    11581 acgcccgtca acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag
    11641 agaatcgata tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa
    11701 gatgatgtca cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa
    11761 atcgctactg ataatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc
    11821 ctgttgctca cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc
    11881 gttccactgc gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat
    11941 actgctgtaa cgttaatttt ctgagtaatg ctgattatgc accaagttgt ctgtgcgacc
    12001 accaatcccg ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga
    12061 tcctgccata ttgcatccgt tgccgtcgag agcggtgtac cggaacaacc ctttggcagt
    12121 gaggaaacgc gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag
    12181 gctgattttt gggtggcgat tgaagctggc attgatggcg acagcacttt cagttgggtg
    12241 gtgattgaaa acgccagcca gcgcggcgag gcgcgttcgg caactttgcc gttacctgcg
    12301 gtgattctgg aaaaagttcg tgaaggcgag gcgcttggtc ccgtgagtcg cgttataccg
    12361 gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc gggaaactca
    12421 ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt cacaatgccg
    12481 tgtatcaggc attgtaggac ggataaggcg ttcacgccgc atccgacatt cggtgcacga
    12541 tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa atcaatcgct tttcagcaac
    12601 acctcttcca gccactgacg cagctccacg ggcgcggctt tcaggctgtt agatccacgc
    12661 gtaatcgtcg cgatgcccgc gccgagttca ttttttaact cacgctgact catttcgcca
    12721 cgcaacagct cttcgacaat acgcacgcga gtccccaacg cttcgcgctc atctggcgtc
    12781 agcatcaggt ttaacaacgg taaatggaga tcgttttggt aggcattctt aagcaggtcg
    12841 acaaaacgta accactcctg gtgacgctgt tctgccatcg ctgctgaata gggtgattgt
    12901 tgggccataa tatgtcgcca ttgttagcgg gggaagcaaa atgactcccc cggttgtact
    12961 cgctaaagag tacgatagca tatcataaac gtgcggatca gtaacgacgt ccccattccg
    13021 tggcgctcat caacgtcggt ttatccccca tgaaatagcg gtagtaagca tcataagcca
    13081 gcacgttctt cacataaccg cgcgtctcgg agaacggaat actctcgaca aatgccacag
    13141 cgtcgatacg cccggcgctg ttgccaagcc aggttcgcac ccgtccaggt ccggcgttat
    13201 aagctgctga ggagaaaata cggttattgc caaactgctg ataaacatat tgcaggtaac
    13261 tggtgccaat gttgatgttc gtttccggat ccagcaattg cccagggctg ctataaccag
    13321 gaatagagaa catcttcacc gtatgggtcg ctgtaccagg cataatctgc atcaggccgc
    13381 tggcccctac cggtgatttc actttcggat tccaggcgct ctcctgacgg gcaatcgcca
    13441 tcgcatagct ttgcgggatc tctttcccac tggtgtaacg tttgaaaaga tcgttgtaag
    13501 ccagcgggaa tcgctcttcc agatgatccc aaagcttccc ggcgatcgtt gcctgaacgc
    13561 taagatccca ccattggtta ttgaaagcat accgagccag ttgagcctgc tctgtttttg
    13621 acttgctctt caccagattg gcccactcgc tacgcgcggt attatcgaga ttccagtaca
    13681 tcaactcgcg cacgcgcgcc atctccggcc cctgagtcag ggtgctgtca acattctgcg
    13741 gcgctttatc aatcttcagc tcatactctt caccgatgcg ttgtgctgca accatcgggt
    13801 agaaaccacg ctgttgtatg agttgatgca aaatctcttt cgcttcagct tcacgtccgc
    13861 gttccagcaa taaatccgcc tgccagtaac gccattcatc tttctctttt gcttccatcg
    13921 gcagacgcgc cagccaggta ttcaggccgc gacgatcgcc ggtgccaagc gccattcgca
    13981 cacggcgttc aatcaacgac gtggattgcg agcgcataat ggcgtcatcg cgccatttcg
    14041 cctgctcatc ggtgacatca ttgcccatca aacgccaggc gacgatatcg cgcagctcct
    14101 gaatctgatc ttcattaagc tgctgcgcct gggcaagcga cgggatcatc agccgtgcat
    14161 tttcggcatc ctgccgcgcc acactggcaa acgccaccgc cgccatttga cgggtaaaat
    14221 cggtcgcgcc ggttgtacgc gcgaaggtca gtaccgtatt agggttgttc gccagtgaaa
    14281 tgattgccga ggcgatagtc tggtaatcgg caggcatctg ccctgccagc actgttacca
    14341 ggcctgtgtt acccgctttc atcgccagac ggatacgctc taaatacgcc agcggatctt
    14401 gtttacctga cgcacgccag acgctaaata acttgtcaca ggcgttaggc tggctcttgc
    14461 cggttagcca cagctctttc gccccttgcc aggcttcttc actctgcccg gtgttccatt
    14521 tcgcatagta gtaattacat tgcgcttcgg tagttccggg cttttccggg ctaaaggcta
    14581 acaagccacg ccagtcttca cgccgcgcca gttcattgac gaaacgagat tgcagcgtgc
    14641 gagcgggagg aagcgtgggg ttagcgcgaa caaagttagt gaccgtcacc gccggttgat
    14701 tcatcaggtc atcggtgatc tggcggtatt ccaggtaggg ataaagcgga taatccttca
    14761 gtccaggcat catttgttcg accacatcca tctgtcgatt atcccaggcc tgcttgattt
    14821 gcgcgtaacg gctacgctgc tcatccagtg agtcggctcg cgccacgctg ctgaccgtca
    14881 gcagacagac accggcagcc aacagccgcc aggtaacttg tttggctttt tccacaagcg
    14941 catcctctaa gtgtaaatac atcaatgcag cgtcatgccg cgtaatagat ttatgctaac
    15001 cagtcattgc cgtttacgcc acgttacgga cactttttta cttttactgc gaaggtgatc
    15061 gaccaactgg gattaggcgg ggaaacaggc tacacttcgc ctttgaaaac tcatcatcac
    15121 gataaacata gaggcgaagt ccaacgtggc tcaattcgtt tataccatgc atcgtgtcgg
    15181 caaagttgtt ccgccgaaac gtcatatttt gaaaaacatc tctctgagtt tcttccctgg
    15241 ggcaaaaatt ggtgtcctgg gtctgaacgg cgcgggtaag tccaccctgc tgcgcattat
    15301 ggcgggcatt gataaagaca tcgaaggtga agcgcgtccg cagccagaca tcaagattgg
    15361 ttatctgccg caggaaccgc agctgaaccc ggaacacacc gtgcgtgagt ccattgaaga
    15421 agcggtttca gaagtggtta acgccctgaa acgcctggat gaagtgtatg cgctgtacgc
    15481 cgatccggat gccgattttg acaagctggc cgctgaacaa ggccgtctgg aagagatcat
    15541 tcaggctcac gacggtcata acctgaacgt acagctggag cgtgcggcgg atgcgctgcg
    15601 tctgccagat tgggacgcga aaatcgctaa cctctccggt ggtgagcgtc gtcgcgtggc
    15661 gttgtgccgc ctgctgctgg aaaaaccaga catgctgctg ctcgacgaac cgaccaacca
    15721 cctggatgcc gaatccgtgg cgtggctgga acgcttcctg cacgacttcg aaggcaccgt
    15781 ggtggcgatt actcacgacc gttacttcct cgataacgtt gcaggctgga tcctcgaact
    15841 ggaccgcggt gaaggaattc cgtgggaagg taactactcc tcctggctgg agcagaaaga
    15901 tcagcgtctg gcgcaggaag cttcacaaga agcggcgcgt cgtaagtcga tcgagaaaga
    15961 gctggagtgg gtacgtcagg gaactaaagg ccgccagtcg aaaggtaaag cacgtctggc
    16021 acgctttgaa gagctgaaca gcaccgaata ccagaaacgt aacgaaacca acgaactgtt
    16081 tattccacct ggaccgcgtc tgggcgataa agtgctggaa gtcagcaacc tgcgtaaatc
    16141 ctacggcgat cgcctgctga ttgatgacct gagcttctcg atcccgaaag gggcgatcgt
    16201 cgggatcatc ggtccgaacg gcgcgggtaa atcgaccctg ttccgtatga tctccggtca
    16261 ggaaccgccg gacagcggca ccatcacttt gggtgaaacg gtgaaactgg catcggttga
    16321 tcagttccgt gactcaatgg ataacagcaa aaccgtttgg gaagaagttt ccggcgggct
    16381 ggatattatg aagatcggca acaccgagat gccaagccgc gcctacgttg gccgctttaa
    16441 ctttaaaggg gttgatcagg gtaaacgtgt tggcgaactt tccggtggtg agcgcggtcg
    16501 tctgcatctg gcgaagctgc tgcaggttgg cggcaacatg ctgctgctcg acgaaccaac
    16561 caacgacctg gatatcgaaa ccctgcgcgc gctggaaaac gccctgctgg agttcccggg
    16621 ctgtgcgatg gttatctcgc acgaccgttg gttcctcgac cgtatcgcca cgcacatcct
    16681 ggactaccag gatgaaggta aagttgagtt cttcgaaggt aactttactg agtacgaaga
    16741 gtacaaaaaa cgcacgctgg gcgcagacgc gctggagccg aagcgtatca agtacaagcg
    16801 tattgcgaag taatgcgtaa aatgccggat gcggcgcgaa cgccttatcc ggcctacaaa
    16861 accagcaatt tcaatacgtt gtaaaactgt aggcctgata agacgcggta agcgtcgcat
    16921 caggcattga gcaccgaatg ccggatgcgg cgtgaacacc ttttccggtc tacaacagcc
    16981 ttagcatcct caatttcgtg gcgggtcacc cgtgcaggtt ttttcaaacc ttgtttaatc
    17041 tcgacagcct cttccagaga ggttttcaac tcatcaaaaa aactcatttt atacctcgcc
    17101 tttgagcagt ttcgtcacgg ttatccttgc tcccccatca tctcccgcac cagctccacg
    17161 cagcgcagga aacggctgtc gtaatcgtcc tcttcaaccc gcacgaattc gatattgttc
    17221 tcttccagca tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg
    17281 ctgcgtaaac catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa
    17341 cggtattcat caatcagcgc ctgcacgaac ggatgctcac gcccttcgta ctttttgcag
    17401 aacgcctggg tagtgacaaa atcggtgtca ataaacgcca ctttattggc gtatttcact
    17461 gcaaaatcaa tgtattgtgc gtgacccaac gcgattttat cgtagtcgga atactgcaat
    17521 gcgatttcat caccgccgag gtgtgaaaag acataatcgc ggccatattc ccacgcactg
    17581 gtggtgttga agatattggc aagtttgttt accagggtgg atttaccgct cgactcgccg
    17641 ccaaggatcg ccacggtacg cacaaaaaac ggcttcactt cggtaggaat atattcccag
    17701 tagcggaacg ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga
    17761 tcgaccagca ccgtctcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc
    17821 gaggtgtaga tcaggtccgg ctggatcccc ttttcagcca taaacttttt gatgccgttg
    17881 ctccacacat cccagccatg cggatacggc tccatgccct cttcgttgaa agcatgaata
    17941 cggatatttt tctgatattt aaaggtttgc aatagccagc gcagacgatc cggcaccgtg
    18001 ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc
    18061 ataatgatat gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg
    18121 gtatgcagcg ggtagaactt accgaatacg acaccaatgg ttttcttctg ccggggaaat
    18181 tcaagcccca aaaaacggtg caatgcctcc agcttttgcg cgctggggct tttaattttg
    18241 gcattcagta actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt
    18301 agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc
    18361 tccgtatctc tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc
    18421 caaaaatctg catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc
    18481 ggcgaaaacc gtgtttcgcc gcttcagaaa tccgctcctg gccactgggc accgggcgga
    18541 tctcccctgc cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac
    18601 gcaggctgga aaccatcgcc agcagtaacg ctaagtcggc gctggtttcg gttaccttca
    18661 cgccgccgac cacgttcaca aacacatcct gatcggccat ttgcagacca ccgtgacggt
    18721 gcaagacagc cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg
    18781 gattcgccat tatcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc
    18841 cttcccacac caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa
    18901 ttgccgaagg gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct
    18961 cattcaccgc gccgaagcgg tttttatggc tgcgtaaggt gcgaaaacgg gagtcggcat
    19021 cgccatccag aagcaccgaa cagtcgatac agtgttccag cactttcggg ccagccagcg
    19081 aaccatcttt ggttacgtgt ccaaccatga caatcgccac accgcgcgtt ttggcgaaac
    19141 gcgtcagata agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat
    19201 ccgccatatg catcacctgg atcgagtcaa ttaccatcag cttcggttgc tcttcttcgg
    19261 caatcaggca gatctgctcg atgctggttt ccgacaacat attgagattg tcagtcggca
    19321 ggccaaggcg atgagcgcgc attgccacct gttgcagcga ctcttcgccg gtgacataca
    19381 gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag caacagcgtg gatttccccg
    19441 caccagggtt accgccaatc agaatggcac ttcctggcac cacgccgcct cctagtacgc
    19501 ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga
    19561 gtttctggac ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg
    19621 ccaccgttgg cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac
    19681 tgcactgccc ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt
    19741 ttggagcttt tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tgacgggcag
    19801 gcaattactt ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt
    19861 gacggatggt gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca
    19921 gccctgctgc tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg
    19981 ccagcgggat ttcatactcc tgcgcgaggc gagtcagagt tttcgctttg tactgcgcgt
    20041 ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg
    20101 ctaccacggc tgtcaggcgc agcttgtcgc gcaggtattc agcaaagaaa gtaaagccgc
    20161 cggaggcaat cgccactttc cagcccagcg tttccagctt gagcaccagc tgcgttaagc
    20221 ctggcatcag cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca
    20281 gcgtcgccac gcggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt
    20341 cggttacctc cgccaccatc tcgcccgttc cggccagctt ggcaatttca tcaatacatt
    20401 caatctggat ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg
    20461 ggattttccc cagcggggcg acatccagct gcgcttcgtg ggccaggcgt gtagcccgtg
    20521 cggtgagtga acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa
    20581 tcaccatcgc cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac
    20641 gaccatacag cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac
    20701 cacttaatga aagaggcaga cccggccata aagagacatc ttcaggcagg tcgcaccagg
    20761 taatgttagg cattaaggct cctgtaaaat cgttcgaagc agggaaaata acgcatgagg
    20821 ctaccttgta tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat
    20881 ggctcgcaca aaactgaaat tccggctaca tcgggcagtg attgtcctgt tctgtcttgc
    20941 cttgttagtg gcactgatgc agggggcgtc atggtttagt caaaaccacc agcgacagcg
    21001 taatccacag ctggaagaac tggcccgcac cctggcgcgt caggtgacgc tgaacgttgc
    21061 accgctgatg cgtaccgact caccggatga aaaacgcatt caggcgatcc tcgatcagtt
    21121 aacagatgaa agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc
    21181 acgttctggc gaaagcgtcg aagtgcgcga tcggctggcg ctcgacggta aaaaagcagg
    21241 cggctatttt aaccagcaga ttgtcgagcc aattgcgggt aaaaacggac cgctcggcta
    21301 tctgcgcctg acactcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac
    21361 cactaacatt ttacgcctga tgttgctgct ctcactggca atcggcgtag tgctgacccg
    21421 cacgctgcta cagggtaaac gcacccgctg gcagcaatcg cccttcctgt taaccgccag
    21481 caaaccggtg ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa
    21541 ggaaatcgtt atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa
    21601 cctggcggtg gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct
    21661 ctggcgcaat gccgacacgg tagtaattgg tcgcgcgcag aacccgtgga aagagtgtaa
    21721 tacccggcgg atggaagaag ataacgtccg cctggcgcgg cgcagtggcg gtggcggcgc
    21781 ggtgttccac gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga
    21841 taaaactatc tccacgtcga ttgtgctcaa tgcgctgaac gcgctcggcg tcagcgccga
    21901 agcgtccgga cgtaacgatc tggtggtgaa aaccgtcgaa ggcgaccgca aagtctcagg
    21961 ctcggcctat cgcgaaacca aagatcgcgg cttccaccac ggcaccttgc tactcaatgc
    22021 cgacctcagc cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg
    22081 cattacttca gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggctcaccat
    22141 gagcaggttt gcgaggccat aaccgaggcc tttttcgccc attatggcga gcacgtggaa
    22201 gcggaaatca tctccccgga caaaacgcca gacttgccaa acttcgccga aacctttgcc
    22261 cgtcagagta gctgagagtg gaacttcggt caggctccgg cattctcgca tctgctggat
    22321 gaacgcttta gctggggcgg cgtggaactg catttcgacg ttgaaaaagg ccatatcacc
    22381 cgcgcccagg tatttaccga cagcctcaac cccgcgccgc tggaagccct cgccgggcga
    22441 ctgcaaggct gcctgtaccg cgcggatatg ctgcaacaag agtgcgaagc gctgttggtt
    22501 gacttcccgg accaggaaaa agagctacgg gagttgtcga cgtggatagc gggggcggta
    22561 aggtaactcg ctgattaaaa tatccaagga aggaaatatg atcgatctgg aaaaccagga
    22621 acgggagatt atcaatttaa tgttcagcca gcgtatttcc tggctagccg ccgtgcgtat
    22681 caggcataaa ttatcgcttg ctgaagtaag taaaatgtta ggtatctcga ttaattctct
    22741 aaaacagatc gaaaagacgg aacggcttag ctcaaacatc aaaagcaaaa tggcagaaat
    22801 ttatggttgt ccaccagagt tgctgatatg cccttcctgg atgacggctg aacataaatg
    22861 aatagattcg tgaatagata cttgaatgtt ttttattttc tatccatatg aaaaataata
    22921 gtaaatctat aaatcagttg ctattgatga atagatacta aaaagccgga gcagtccccc
    22981 actccggctt aacacgacaa tcgcctttca gcgaaacaca attactcttt atcgcccagc
    23041 agaacggatt ccagtgcgat tttgatcatg tcgttgaagg tggtctgacg ctcagcggca
    23101 gtggtctgct cgtgagtgcg gatgtggtca gacacggtgc agatggtcag ggctttcgcg
    23161 ccaaactctg cagccacgcc gtagatacca gccgcttcca tttccacgcc gaggatgccg
    23221 tatttttcca tcacgtcgaa catttcgccg tccggagagt agaacaggtc agcggagaac
    23281 aggttaccca cgcgagcatc aacgcccagt gctttagctg catctactgc gttacgcacc
    23341 atgtcaaagt cagcgatagc ggcaaagtca tggtctttaa aacggatgcg gttaactttg
    23401 gaatcggtgc aggcccccat accgataacg acgtcgcgca gttttacgtg cggcagaact
    23461 gcgccacagg aaccaacgcg gataattttc ttcacgccga aatcggtgat cagttctttg
    23521 gtgtagatgg agcaggacgg gatacccata ccgtgaccca ttacggaaat tttgcggcct
    23581 ttgtaagtac cagtgaagcc cagcataccg cgaacgttgt tcacttcacg ggcatcttca
    23641 aggaaagttt cagcaatata cttcgcacgc agcgggtcgc ctggcatcaa aactacgtca
    23701 gcgaaatcgc ccatttctgc attaatgtgt ggggtagcca ttgttttatc ctttttgtga
    23761 cataacaaag gcggagtcaa cgctccgccc aaatccatca gaacatggct ttgccatatt
    23821 ccatatcaga agtaccaaaa tattttgcca gagtctggcc gatatccgcg aaggtttcac
    23881 ggtgacccag tgagcccggt tttactttcg ggccatatac cagtaccgga atgtgttcac
    23941 gcgtgtggtc agtaccggtc caggtcggat cgcaaccgtg gtcagcggtg aggatcagga
    24001 tgtcgtcatc gcgcagcaga gacatcagct ccggcagacg gcggtcgaac agttccagac
    24061 ccgcagcata accggcgacg tcgcggcggt ggccccagga agagtcgaag tcaacgaagt
    24121 tggtgaagac gatggtgtta tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca
    24181 gcgcgtccaa gccagtcgct ttcacttttt tggtgatacc gcagttggcg tagatgtctg
    24241 caattttacc gacagaaacc acctggccgt gtttttcatc aaccagtttc tgcagcacgg
    24301 tcggtgctgg cggctcaaca gccaagtcgt gacggttacc ggtacgctgg aagttaccag
    24361 ctttgtcacc gataaacgga cgagcgataa cacgaccgat attgtagccg ccgttggtca
    24421 gctcttcacg ggcgatttcg cacagttcgt agagtttatc cagaccgaaa gtttcttcat
    24481 ggcaggcaat ctggaacacg gagtcagcgg aggtatagaa aatcggcttg ccggttttca
    24541 tgtgctcttc gcccagttga tccagaatga ccgtaccgga agagtggcag ttaccgaggt
    24601 aacccggcag attagcgcgt tcgaccagtt tatccagcag ctcttgcggg aagctgtttt
    24661 cgtgatcgga gaaatatccc cactcaaaca gaaccgggac accggcgatt tcccagtgac
    24721 cagacggggt atctttaccg gatgacattt cgtgcgccca tgcgtacgcg ccgataactt
    24781 cagcgttgcc gtccattccc gccggaatga aaccggtaga accttcgtgt gctttcgcca
    24841 gccccagacg ggtcagattt ggcagattga gcggcccttt acgaccatta tcagcttcgc
    24901 ctttggcaca agcttctgcg atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt
    24961 ctgcatcttc tgtagcgccg atgccgaatg agtccagcac cataataaat gcacgtttca
    25021 tatgttctcc gtactcagtg cttcaaattt aaatgatcag atcagtatac cgttattcgc
    25081 tgatacggcg atagacagtt ggtgtgcttt ccggtgcgtt atcggcaagt ttaattgccg
    25141 ctttcaccgc tttcgccgct tcctgccagc tgttttcgtc tttcgcgagg ataaccgcca
    25201 gcggacgctg accgtctacc tggtcgccca gacgcgccat atcagtaaag ccgacgctgt
    25261 aatcgatggt gtcagatgcc tgacgacgac cgccgcccat tgcaactact gccatcccca
    25321 gcgcgcgggt atccatttca ctgacaaaac cttcggtatc agcatagact gctttcgtca
    25381 gcatcgctgt cggcagatac ttcgcgtagt tctcaacgaa gtcggtcggg cctttttgtg
    25441 ccgctaccat acgaccaaag acttctgccg ctttaccgtt gtccagcacc gcctgcaatt
    25501 tcgcgcgcgc ttcggcgtca tctttcgcca gtttgccgga gatcaacatc tccacgcaca
    25561 gcgccatcgt gacatcaaac agacgcgggt tacgatactc acccgtcagg aactgcaccg
    25621 cttcacgaac ttcaaccgcg ttacctgcac tggaggccag tacctgattc atgtcggtga
    25681 gcagcgcggt ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct tcggcaaggg
    25741 cttcagagag ttcgtaggtc ggcataaacg cgccgctacc cactttcacg tccatcacca
    25801 gcgcgtccag accttccgcc agtttcttcg ccaggatcga ggcagtgatc agcgggatgg
    25861 agtccacggt tgcggtaata tcacgggtcg cgtagaaacg tttatcagcc ggagccagtg
    25921 aactggtctg accgataatc gccacgccga cgtctttaat aatttcgcgg aaacggttgt
    25981 catccgggaa aatgtcgaag ccagggatgg attccagttt gtcgagcgta ccgccagtat
    26041 gaccgaggcc gcgaccggag atcatcggaa tatagccgcc gcaggctgcg accatcggcc
    26101 ccaacatcag cgaagtgaca tcgccgacgc cgccggtcga gtgtttatca acaatcgggc
    26161 cattcagatg caggcttttc cagtcgagaa cggttcctga atctcgcatc gccatggtca
    26221 gcgagacacg ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa
    26281 tctgcccttc ggaaatagtg ttgtcgcgaa taccgttgat aaagaaacga atttcttcat
    26341 cgctcagcgc atgaccatca cgttttttac gaataatttc ttgtgcgaga aacaaggtaa
    26401 cctcccgagg aaaagagtat gaggcggctt ttgccggatg cgtctacgcc ttatccggcc
    26461 tacgggtaga tacgtctcgt aggcctgata agcgcagcgc atcaggcgta aagcatctta
    26521 cttagtagct gctggcgctc ttaccgtcgc cgtggcccag cgctttcagc aggcttgcca
    26581 gcaggctgga agcaccaaag cggtagtgac gcgcatcagc ccagtcagca ccgaacagtt
    26641 catctgcgat ggcgagatat ttctgcgcat cttccgcagt acgcacgccg cccgccggtt
    26701 tgaaaccaac ggttttttct acgcccatat cacggatcac ttccatcatg atgcgagcgc
    26761 tttccggcgt cgcgttcaca gccactttac cggtagaggt tttgatgaag tccgcacccg
    26821 ctttgatgga gatttcagac gctttacgga tcagcgcttc gtctttcagt tcgccggttt
    26881 cgatgatcac tttcagcagt acattcgctg ccgcgcaagc ctctttacag gctttcacca
    26941 ggtcaaaacc aacctgctcg tcacccgcca tcagcgcgcg gtacgggaac accacgtcaa
    27001 cttcatcagc accgtaggcg attgccgcac gggtttctgc cagcgcgatt tcgatgtcgt
    27061 cgttaccgtg tgggaagttg gttaccgtag cgatacggat ttccggggtg ccctgctctt
    27121 tcagagtttt gcgagcaatc gggataaaac gaggatagat acagatagcg gcggtattgc
    27181 cgaccggagt tttggcctga tgacacagag caattacttt ctcgtcggtg tcgtcgtcat
    27241 tcagggtggt caggtccatc aatttcagtg cacgcaggct gcttgctttc agatcagtca
    27301 tttcattctc ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta gtattctaac
    27361 atctactccg caacacactt cgatacacat cacaattaag gaaatctact tacaagtttg
    27421 catcactgta atgcgatctg gttcaaataa ttcactttca aatgaatgcg tcagtggtgg
    27481 caaacgcatc aggatcaaag tgaacatcac gaaacttctt acaatggcgc atcaccttcg
    27541 gcataaaagg aaaagaagat gcccacttct catgaaaatg cactgcaaca acgttgccag
    27601 caaattgtca ccagcccagt gcttagcccg gagcagaagc gccattttct ggcactggaa
    27661 gcagaaaaca atctgcctta cccacagctt cctgccgaag cccgccgcgc gctggatgaa
    27721 ggtgtaatct gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc
    27781 gattacgctc gttttctggc gaacggttcc gaatggctgg agctggaagg cgcgaaagat
    27841 cttgatgacg cactctctct gctgaccatt ctttaccacc acgtaccgtc ggtcacatcg
    27901 atgccggtct acctggggca actggatgcg ttgttgcaac cgtatgttag aattctaaca
    27961 caaaacgaga tcgatgttcg aataaaacgt ttctggcgtt acctcgacag aaccctgcca
    28021 gatgccttta tgcacgccaa tatcggcccg tctgattcgc ccattacccg cgcgatctta
    28081 cgtgcagacg cgcaggtttc accgaacctg acctttatct acgatcctga aatcacccct
    28141 gatgacctgc tgctggaagt ggcgaagaac atctgtgaat gtagcaaacc gcacatcgcc
    28201 aacggtccgg tgcatgataa aattttcaca aaagggggct acgggattgt gagctgttac
    28261 aactcactgc cgctggcggg tggtggcagc acgctggtac gccttaacct gaaagccatt
    28321 gccgagcgca gcgaatcgct ggatgacttc tttacgcgca ctctaccgca ctactgccag
    28381 cagcagatcg ccatcatcga tgcgcggtgt gaattcctct atcaacaatc acacttcttt
    28441 gagaatagct tcctggtgaa agaagggctg attaaccctg aacgttttgt gccaatgttt
    28501 ggcatgtatg ggctggcgga agcggttaac ttgctgtgtg agaaagaagg gattgccgcg
    28561 cgctacggta aagaagccgc cgcaaatgaa gtgggttatc gcatcagcgc gcaactggcg
    28621 gagtttgtcg ccaatacccc cgtgaaatat ggctggcaaa aacgcgccat gttacacgca
    28681 cagtcgggga tcagttccga tatcggcacc acgccgggcg cgcgtttgcc gtatggcgat
    28741 gagccagatc cgatcaccca tctgcaaact gtcgccccgc atcatgctta ttattattcc
    28801 ggcatcagcg acattctgac gctcgacgaa accatcaaac gtaacccgca ggcactggta
    28861 cagctttgcc tcggtgcctt taaagccgga atgcgtgaat ttaccgccaa tgtcagcggt
    28921 aacgatctgg ttcgcgttac cggttatatg gtgcgtttgt cggatttaga aaaatatcgc
    28981 gccgaaggtt cacgcaccaa caccacctgg ctgggcgaag aagccgcacg caacactcgt
    29041 attctggaac gccagccgcg cgtgataagc catgaacagc agatgcgctt tagtcagtaa
    29101 gattatcccc ttctcctgcg ttgacgggcc aggcagtcgt ctggctctgt ttttgcaggg
    29161 ctgcaatctg cgctgcaaaa actgtcacaa tccgtggacg atgggacgtt gcaatgactg
    29221 tggggagtgc gtgccacagt gtccgcatca ggcgttgcag attgttgacg gcaaagtggt
    29281 gtggaacgct gtggtctgcg agcaatgtga tacctgcctg aagatgtgtc cgcaacatgc
    29341 cacaccaatg gcgcaatcca tgagcgtgga cgaagtgctt agccatgtgc gcaaagcggt
    29401 gctgtttatt gaagggataa cggtaagcgg cggcgaagcc acgacccaac tgccgtttgt
    29461 agtggcgctg tttactgcta tcaaaaacga tccgcaactg cgccatctca cctgtctggt
    29521 ggacagtaac ggtatgttga gcgaaaccgg ctgggaaaaa ttactcccgg tgtgtgacgg
    29581 cgcaatgctc gatctcaaag catggggtag cgaatgtcat caacaattaa ccggacgcga
    29641 taatcaacag attaagcgca gcatctattt gctggcagag cgcggcaagc tggcggaact
    29701 gcgtttgctg atgatcccag gccaggtgga ttatttgcaa cacatcgaag aactggcggc
    29761 gtttatcaag ggacttggtg atgttccggt tcgcctgaac gcgtttcatg cccacggcgt
    29821 gtatggcgag gcgcaaagct gggcgagcgc cacgccggaa gacgttgagc cgttggctga
    29881 tgcattaaag gtgcgcgggg tgagccggtt gatatttccg gcgctctatt tgtgacatgc
    29941 cggatacggc ctacggttcg gcacagactt gtaggcctga taagacgcgt caagcgtcgc
    30001 atcaggcatt gtgcgccaac tgccggatgc ggcgtgaacg ccttatccgg cctacggttc
    30061 ggcacaaacc tgtaggcatg ataagacgcg ttaagcgtcg catcaggcat tatgcgccga
    30121 ctgccggatg ctgcctacga agggcaaact acccacgaac gttaaacacc gcatacgtgt
    30181 tattaagcaa cacttcggca atctcatccg cgggttccgg gcgtaactcg caaagcacgg
    30241 caaacacacg ggcagcctgt tccgggcggt taggctgccc ctgaaaaccg ttgagcggca
    30301 tatccggcgc atcggtttcc agcaacaacg atgccagcgg taattttgcg atgacatcgc
    30361 gggttttact ggcgcgtgga taggtgatag taccgcctac gccgattttg tagcccagct
    30421 gtacaaaccg ttcagcctgt tgctggctgc cagaaaatcc gtggacaaca ccagcacgcg
    30481 gcaaatcgtg gcgtttaaga tgcatcgcca gtttgtcgtg cgtgcgccgt gaatgcagga
    30541 tcaccggcag atcgtagcgt ttcgccagtt tcagttgttc gtcgagtaac cactgctgcc
    30601 tctcaaattg cggatcgtcg ccgaagagat ccagaccgat ctcccccacc gccaccacct
    30661 tcgccggacg cctttccagc gcctgctgta gctgctcaag agacacatcg ctatgttttt
    30721 ccaacatacc aggatgcaag cccaatacgg catacagcgg ttgataattt tccgctaacg
    30781 cctgcacacg ggcaaaattt tccgcctctg tcgccggaac aatgatcttg cctacgcccg
    30841 cttgtgccgc gcgttgcagg ctggcctctt catcgccaat aaaaggcggg aaatcaaaat
    30901 ggcagtgagt gtcgataaaa cggcaaatca agccagatcc tcattgttaa atgtcgggtc
    30961 gttcgcctgc ggagcgtcgc tcacttcggc aaccagcgta tcgttggcga ctggcgcagg
    31021 cggaatgaca atcgattccg gcgtcaccac tggcaccaga tggcgggtaa gcggcgcttt
    31081 ttcagttaat agcttgccaa ccgtggcgag gaaataacga ccgcataaac gcccaagttt
    31141 atagtcttca cgcagcgccg gaatccgact gccaagcgcg atactatgta atggcttcgg
    31201 cggataaatt tcggatatcc gcagcttgcc cggtggtttc tcaataaatt gctgaatgtc
    31261 acgatagctg gtttcatgat gctgcaccag attgaccagc ggctgcaggc tactgtcacc
    31321 cagccagcgt tccatgcgtt tgaaccactg cggcgtgtag tacatttgtg acggcacagt
    31381 gcggatgacg accaacgttt tagcgccctg ccttgccgcc tctttaaccg gaatcgcatc
    31441 actgatcccg ccatccaggt agttaatgcc ttccagcgac actccgctac gataaaagcc
    31501 aggtatcgca ctggaggcgc gaatcacatc cagccagttt tgtttggttg gtaaaaagta
    31561 attcggcgcg tagtcatcct gacgacaggc gcacatataa aacgatttgc cgctgtcaaa
    31621 caaccgcgcg gcggtatcca tttgcaacgg catctggctt gcggtggcct ccaccagcca
    31681 gtcgagatcg ataagatttc ctccacggac aaagcgcaat ggatcgaaaa attcgcgttt
    31741 tgtggtatag cgcatgatga ctttgcgcgc gtaaccgggc tgattgcaga taaacgccga
    31801 gaggttctgc gccccggcag atgtgccgag ataaagatcg aaaggattaa actgcgcgcg
    31861 cataaactca tccagcacgc cagccgtgaa aattccacgc tgtccgccgc cttcgcacac
    31921 cagagctatt cgtccaggct ggaacggcct tagcgacaac ggcgcaatat taccaagcgt
    31981 tacaggtatt cgctgcccca cttctgcctt cctgtttttg ttgtgaaaga aagacaaagt
    32041 aacgcaattt attattcgct aaaaccgcaa aagccagtcc gctggactgg ctttggataa
    32101 aattttctta atggtctgaa atctagggtc gttttcggcc catgaacaaa ctcactagga
    32161 acagaataat cccgacgaca aagacaattt tagctgcgcc tgcagcggta ccggccagac
    32221 caccaaaccc aagtgcggcg gcgattaacg cgataaccag aaatatgatg ccccaacgaa
    32281 acataagcgt ctcctttacc atagttaatg tcaccgcttg atatgagcgt gaaaatcact
    32341 caatgcgata tttttagtgt ggtgcacatc gcgcctcccg acaaagttcg ggaggacgaa
    32401 ttacttagtt ttcagatcat ttttaacgct tttcacacca tctaccgctt tggcgatact
    32461 ttcagcacgg tcactttgtg cctgagaatc tacggtaccg gagagctgaa ccacgccatc
    32521 ggtggtttca actttcactt tacgtgaagg gacgatatcg tccgccagca gtttggcttt
    32581 gatttcactg gtggtggcgg tgtcacccgc gtagcccttc accgagcctt ctttagcgtc
    32641 gcgaacgtgc agtttgtcgc tgacagaggt cacgccttca acgcctttcg ccactttcac
    32701 tgcctcttcg gcctgggcct gactttcaac gaaaccgctc agggtcacga ctttttgatc
    32761 ggttttgaca gagatatcgg tgctcttgat gttgtcatga tccaccagcg ccgccttcac
    32821 tttcgcggtg atggcgctgt catccatgaa attaccgact ttattcatag agctatcgac
    32881 tttttgccct gcgctttcat tggtagtctg cgcgttgttt tccgcgtagg cagagccggt
    32941 cgcgacggca gaggtcaaca ttacagccag cagagttttc gaaatcttca gtcttgtcat
    33001 agtcatcgat ttattcctgt atgtttgctc gtaatttgag cctagcaaca cgaggttgca
    33061 ttgctgaata gggagagact tcaccctcta cagaagtcaa tggtcgccat cacaaaagcg
    33121 atgggtgatg aataacgacc attacagcct ctgaatcagt tattaatatc ggtagaatga
    33181 caatcgacgg ctttagatac taatatctac gcattgaacg gtatttaacg ccatcagaaa
    33241 tgtcatcact ttgttaaata tagatcacaa ttttgaaacc gctcgggata tcagcgaaaa
    33301 cataagcaaa agtgaatgtt ttaagaacat tccgtaagcg gctaataagg aagggaaatt
    33361 gacagggcgc agcggctacc gcgccctgga ggcaagaatt aatgctcgcg ggtctggtgg
    33421 aactgaacgt ccggataacg ttcctgtgcc aggcgcaggt tgaccatgct ggtagcgatg
    33481 taagcgaggt tatcgccgcc atcaagcgcc agttggcttt cgttcttacg cttgaactct
    33541 tcgaatttct tcgcgtctgc acattctacc cagcgggcag tggcaacgtt gactgactca
    33601 tacactgctt caacgttgta ttcgctcttc aggcgcgcta ccaccacatc aaactgcagc
    33661 acaccaaccg caccaacgat cagatcgttg ttggagattg gacggaacac ctgcaccgcg
    33721 ccctcttcgg aaagctgtac cagccctttg agcagctgtt tttgcttcag cggatctttc
    33781 aggcggatac gacggaacag ttctggtgcg aagttcggaa taccggtgaa cttcatcatc
    33841 tcaccctggg taaaggtgtc gccgatctga atggtgccgt ggttgtgcag gccgaggata
    33901 tcgcccggat acgcttcttc aacgtgcgaa cggtcacccg ccataaaggt cagcgcgtcg
    33961 gagatcacca catctttcgc agtgcgcacc tggcgcagtt tcatgccttt ttcatattta
    34021 ccggacacca cacgcataaa cgccacgcgg tcgcggtgtt tcggatccat gttggcctga
    34081 attttaaata cgaagccggt aaatttatct tcgctcgctt ctacggtacg ggtatcggtc
    34141 tgacgcggca tcggcgcagg cgcccactcc accaggccat tcagcatatg atcaacgccg
    34201 aagttaccca gcgcagtacc gaagaatacc ggagtgattt cgcccgcaag gaacagctct
    34261 ttgtcgaact cgttagacgc gcctttaacc agttccagtt cgtcacgcag ctgctgtgcc
    34321 agatcttcac caaccgcagc atcgagatcc gggttattca gccctttaac aatgcggact
    34381 tcctgaatgg tgtgcccttt accgctctga tagagatagg tttcatcttt ataaaggtgg
    34441 taaacgcctt taaacagctt gccgcagcca attggccaag tgatcggtgc gcagccaatt
    34501 ttcagctcgt tctcaacttc atcgagcagt tccatcgggt cgcggatatc acggtcaagt
    34561 ttgttcataa aggtgaggat cggcgtgtcg cgcagacggg taacttccat cagcttacgg
    34621 gtacgatctt caacaccttt tgcggcgtcg ataaccatca ggcagcagtc caccgccgtc
    34681 agggtacgat aggtatcttc cgagaagtct tcgtgccccg gggtgtcgag caggttaacc
    34741 aggcaatcgt gatacggaaa ctgcatcaca gacgtagtaa tggagatccc acgctgcttt
    34801 tccatctcca tccagtccga cttagcgtgc tggttggaac cacggccttt tactgtaccg
    34861 gcggtctgaa tggcctgtcc gaacagcagc accttctcgg tgatggtagt tttaccggcg
    34921 tccgggtgag aaataatggc aaaagtgcgg cgcttcgcca cctctttcaa ataaggagac
    34981 aacgtcataa ttctttcttc ttgagtaagc ggcatcgcgc cgcgcttgtt gaaaacgaaa
    35041 aattgcggct attttaccca tcaacggggg ggaggcaatc agtgtttaca caggagctgc
    35101 tccagttcgt gcaacgaaga aacggtccag gtgggcgcga tgccttctgg ttgctcgcga
    35161 ttgtgcgcat tcagccagca ggtcgcaagc ccggcgttga tgccaccgag aatatcggac
    35221 tcggcagtgt cgccaaccat cagcacgcgt gaacggtcag gattgcccgc ctgttccagc
    35281 gcataatcga aaattttctt attcggtttg gcaacgccaa cttcttcgga aatcaccagc
    35341 aaatcgaagt aatcacgcag gcccgtccgt tccagacgca cttgctgcaa ggcgctaaaa
    35401 ccgttggtga tgatgccgat tttagcgttg ctacgaatgg cgttaagcag agaaaccgcg
    35461 cccggcaacg gcgtacagat ttccgccatc gcattaataa aggcatcgtt aagcaagcct
    35521 gcttcaactt tcagccgttc ggcccagctc tcgaaacgcc cgtgctgaag ctgtaatgaa
    35581 gtgatcgcgc cgttttgata atccacccac agtggcttgt taacggcctg atagtcctga
    35641 aaatcttcag cggtaaaggt gacgctgtaa tcaagaaaca tccgctgcag gccggtgaat
    35701 gagtcaaagg taaacagcgt ttcatcggca tcaaagaaaa tccagtccca cttcattatt
    35761 ccaccttgtc ttacatactg attggcaacg ccatgatgat ggcgtcttcg cgaccgtccg
    35821 tggtggggta gtaattgcgg cgaatcgtcg cctcgttaaa gcctaaactt tcgtacaggg
    35881 caatggcggc agcatttgag gcacggactt ccagccatag tgtcgccacg ccgcgttttt
    35941 ccagttcgtc gatcagatgt tccagcagcg ccctacccag cccctgacgc tgatagtcgg
    36001 gatcgaccgc aatattgaac aatgtagctt catccagcac cacttgcgta atcgcaaacg
    36061 ccgccatttt gccgttttgc gttaactgaa agttgagata acgctcgccc tggttgctgg
    36121 caaaggtttt ttcactccac ggaaaggcgt gggcgcgttg ttcaatgtgg taagccgctg
    36181 gtaaatcagt cgtttcgagg gaagaaatcg tgttcatatg tgcaaatttg ttgccataac
    36241 gcggcgcgtg ccgttgggtt tgcccgtaat tcggtgagcg ccggtgatgc cacctgagcg
    36301 ccttccaatg atagcggttc gtcagtaccc aaccgccagc tgttgcagcg actgccttgc
    36361 ggcagcatcg cgattttttc tggcgtcagt tgcagcactt gatcggggct gacggttaat
    36421 gcgcgcagaa catcgctcac taaaggatca gtcagggcgg gaagatcgtt tgccaccatc
    36481 accagacgga cgtgtgccgg gctcgcaatg gcaatctccc cctgcaacgc gccagggcga
    36541 cgcagcgacc actgggtaat gcccagttgc tgtaactgcc agtctcgtcg ggatgtcata
    36601 gcgaatcgct cctgttgtca ggggcgcaaa tatagcaaat tcgtcgatac cgcgccaaca
    36661 tatggctata atcgccgcca gtatcaattg aggagcattc catgtctgca tttaccccgg
    36721 caagtgaagt cttgctgcgt cacagtgatg atttcgaaca aagccgtatt ctgtttgccg
    36781 gagacttaca ggatgacctg cccgcgcgtt tagataccgc ggccagccgt gctcataccc
    36841 agcaattcca ccattggcag gtgttaagct gccagatggg ggataacgcc cgttttagtc
    36901 tggtcgccac ggcgaatgat gtcgcagatt gcgatacgct gatttactac tggccgaaga
    36961 acaaaccgga agcccagttc cagttgatga atttactttc tctgctgcca gtggggacag
    37021 atatttttgt cgttggcgag aaccgcagcg gcgtgcgcag cgctgagcag atgctggcag
    37081 attatgcgcc gttgaataaa gtcgacagcg ctcgtcgctg tggcctctat tttggtcgtc
    37141 tggaaaaaca gccagtattt gatgcggata aattctgggg cgaatacagc gtcgatggcc
    37201 tgacggtcaa aacgctgcct ggcgtgttta gccgcgacgg tctggatgtc ggtagccagt
    37261 tgctgctctc gacgttaact ccgcacacga aaggtaaagt gctggatgtc ggctgtggcg
    37321 cgggtgtact ttcagttgcc tttgcgcgcc attcgccgaa aattcgtctc accttgtgcg
    37381 atgtctctgc cccggcggta gaagccagcc gcgcaacact tgcagccaac ggtgttgaag
    37441 gtgaagtctt tgccagcaac gtcttttccg aggtgaaagg ttgttttgat atgatcatct
    37501 ccaacccgcc gttccacgat gggatgcaaa ccagcctgga tgcggcgcaa acgctgattc
    37561 gtggcgcggt gcgtcatctt aatagcggcg gcgagctgcg aattgtagcg aacgccttcc
    37621 tgccttaccc ggacgtgctg gatgagacat ttggcttcca tgaagttatc gcgcaaaccg
    37681 ggcgcttcaa ggtatatcgc gccattatga cccgccaggc gaagaaaggt tgagtatctg
    37741 atatctgtcg ccgcataccg tgtcggatgc ggcgtgaacg ccttatccga cctacaaaac
    37801 ctgcacgtta gcccttcgta ggccagataa gacgcgccag cgtcgcatct ggcatctgcc
    37861 cccgcatgcc taccaaacct gcacgctagt ttcctgatgg acatttttcc agcaattaca
    37921 catcaggcga taattaacta ttgacgaaaa gctgaaaacc actagaatgc gcctccgtgg
    37981 tagcaattct ttttaagaat tgatgg
//
LOCUS       DAJTRN010000029        37879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRN010000029 DAJTRN010000000
VERSION     DAJTRN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..864
                     /gene="hisG"
                     /locus_tag="OO328_001542"
     CDS             <1..864
                     /gene="hisG"
                     /locus_tag="OO328_001542"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5930148.1"
                     /translation="KSGRLSDDSRELLARCGIKINLHTQRLIAMAENMPIDILRVRDD
                     DIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFGGCRLSLATPVDE
                     AWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPRAGLADAICDLVS
                     TGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQGVIQARESKYIM
                     MHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWETMEKLKALGASS
                     ILVLPIEKMME"
     gene            870..2174
                     /gene="hisD"
                     /locus_tag="OO328_001543"
     CDS             870..2174
                     /gene="hisD"
                     /locus_tag="OO328_001543"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5930149.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2171..3241
                     /gene="hisC"
                     /locus_tag="OO328_001544"
     CDS             2171..3241
                     /gene="hisC"
                     /locus_tag="OO328_001544"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5930150.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3241..4308
                     /gene="hisB"
                     /locus_tag="OO328_001545"
     CDS             3241..4308
                     /gene="hisB"
                     /locus_tag="OO328_001545"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5930151.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4308..4898
                     /gene="hisH"
                     /locus_tag="OO328_001546"
     CDS             4308..4898
                     /gene="hisH"
                     /locus_tag="OO328_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5930152.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            4898..5635
                     /gene="hisA"
                     /locus_tag="OO328_001547"
     CDS             4898..5635
                     /gene="hisA"
                     /locus_tag="OO328_001547"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5930153.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5617..6393
                     /gene="hisF"
                     /locus_tag="OO328_001548"
     CDS             5617..6393
                     /gene="hisF"
                     /locus_tag="OO328_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5930154.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6387..6998
                     /locus_tag="OO328_001549"
     CDS             6387..6998
                     /locus_tag="OO328_001549"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5930155.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7184..8161)
                     /gene="wzzB"
                     /locus_tag="OO328_001550"
     CDS             complement(7184..8161)
                     /gene="wzzB"
                     /locus_tag="OO328_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5930156.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8307..9427)
                     /gene="ugd"
                     /locus_tag="OO328_001551"
                     /pseudo
     CDS             complement(8307..9427)
                     /gene="ugd"
                     /locus_tag="OO328_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(9677..11083)
                     /gene="gndA"
                     /locus_tag="OO328_001552"
     CDS             complement(9677..11083)
                     /gene="gndA"
                     /locus_tag="OO328_001552"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5930157.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11192..11551)
                     /locus_tag="OO328_001553"
     CDS             complement(11192..11551)
                     /locus_tag="OO328_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930158.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(11548..12054)
                     /locus_tag="OO328_001554"
     CDS             complement(11548..12054)
                     /locus_tag="OO328_001554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930159.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12200..12763)
                     /locus_tag="OO328_001555"
     CDS             complement(12200..12763)
                     /locus_tag="OO328_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930160.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(12970..13908)
                     /locus_tag="OO328_001556"
                     /pseudo
     CDS             complement(12970..13908)
                     /locus_tag="OO328_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(13971..14345)
                     /locus_tag="OO328_001557"
     CDS             complement(13971..14345)
                     /locus_tag="OO328_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5930161.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14349..15497)
                     /gene="wzy"
                     /locus_tag="OO328_001558"
     CDS             complement(14349..15497)
                     /gene="wzy"
                     /locus_tag="OO328_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5930162.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(15517..16422)
                     /gene="rfbG"
                     /locus_tag="OO328_001559"
     CDS             complement(15517..16422)
                     /gene="rfbG"
                     /locus_tag="OO328_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5930163.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16415..17305)
                     /locus_tag="OO328_001560"
     CDS             complement(16415..17305)
                     /locus_tag="OO328_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5930164.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17335..18591)
                     /gene="wzx"
                     /locus_tag="OO328_001561"
     CDS             complement(17335..18591)
                     /gene="wzx"
                     /locus_tag="OO328_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5930165.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(18607..19152)
                     /gene="rfbC"
                     /locus_tag="OO328_001562"
     CDS             complement(18607..19152)
                     /gene="rfbC"
                     /locus_tag="OO328_001562"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5930166.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19157..20035)
                     /gene="rfbA"
                     /locus_tag="OO328_001563"
     CDS             complement(19157..20035)
                     /gene="rfbA"
                     /locus_tag="OO328_001563"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5930167.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20094..20993)
                     /gene="rfbD"
                     /locus_tag="OO328_001564"
     CDS             complement(20094..20993)
                     /gene="rfbD"
                     /locus_tag="OO328_001564"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5930168.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(20993..22078)
                     /gene="rfbB"
                     /locus_tag="OO328_001565"
     CDS             complement(20993..22078)
                     /gene="rfbB"
                     /locus_tag="OO328_001565"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5930169.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(22451..23344)
                     /gene="galF"
                     /locus_tag="OO328_001566"
     CDS             complement(22451..23344)
                     /gene="galF"
                     /locus_tag="OO328_001566"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5930170.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(23519..24913)
                     /gene="wcaM"
                     /locus_tag="OO328_001567"
     CDS             complement(23519..24913)
                     /gene="wcaM"
                     /locus_tag="OO328_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5930171.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(24924..26144)
                     /gene="wcaL"
                     /locus_tag="OO328_001568"
     CDS             complement(24924..26144)
                     /gene="wcaL"
                     /locus_tag="OO328_001568"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5930172.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26141..27421)
                     /gene="wcaK"
                     /locus_tag="OO328_001569"
     CDS             complement(26141..27421)
                     /gene="wcaK"
                     /locus_tag="OO328_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5930173.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(27601..29079)
                     /gene="wzxC"
                     /locus_tag="OO328_001570"
     CDS             complement(27601..29079)
                     /gene="wzxC"
                     /locus_tag="OO328_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5930174.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29081..30475)
                     /gene="wcaJ"
                     /locus_tag="OO328_001571"
     CDS             complement(29081..30475)
                     /gene="wcaJ"
                     /locus_tag="OO328_001571"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5930175.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(30530..31900)
                     /gene="cpsG"
                     /locus_tag="OO328_001572"
                     /gene_synonym="manB"
     CDS             complement(30530..31900)
                     /gene="cpsG"
                     /locus_tag="OO328_001572"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5930176.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32093..33529)
                     /gene="cpsB"
                     /locus_tag="OO328_001573"
     CDS             complement(32093..33529)
                     /gene="cpsB"
                     /locus_tag="OO328_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5930177.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(33532..34755)
                     /gene="wcaI"
                     /locus_tag="OO328_001574"
     CDS             complement(33532..34755)
                     /gene="wcaI"
                     /locus_tag="OO328_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5930178.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(34752..35231)
                     /gene="gmm"
                     /locus_tag="OO328_001575"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(34752..35231)
                     /gene="gmm"
                     /locus_tag="OO328_001575"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5930179.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35231..36199)
                     /gene="fcl"
                     /locus_tag="OO328_001576"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35231..36199)
                     /gene="fcl"
                     /locus_tag="OO328_001576"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5930180.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36202..37323)
                     /gene="gmd"
                     /locus_tag="OO328_001577"
     CDS             complement(36202..37323)
                     /gene="gmd"
                     /locus_tag="OO328_001577"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5930181.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37349..>37879)
                     /gene="wcaF"
                     /locus_tag="OO328_001578"
     CDS             complement(37349..>37879)
                     /gene="wcaF"
                     /locus_tag="OO328_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5930182.1"
                     /translation="FSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLF
                     GAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLCT
                     GSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVV
                     CRGNPAVVIRERVETE"
BASE COUNT        10110 a         9515 c         8543 g         9711 t
ORIGIN      
        1 aaatccggcc gtttaagtga tgactcacgc gaattactgg cgcgctgtgg cattaaaatc
       61 aatcttcaca cccagcgcct gatcgcgatg gcagaaaaca tgccgattga tattctgcgc
      121 gtgcgtgacg atgacattcc ctgtctggta atggacggcg tggtagacct tgggattatc
      181 ggcgaaaacg tgctggaaga agagctgctt aaccgccgcg cccagggtga agatccgcgc
      241 tactttaccc tgcgtcgtct ggatttcggc ggctgccgcc tttcgctggc aacgccggtt
      301 gatgaagcct gggacggtcc gctctcctta aacggtaaac gtatcgccac ctcttatcct
      361 cacctgctca agcgttatct cgaccagaaa ggtatctctt ttaaatcctg cttactgaac
      421 ggttctgtcg aagttgcgcc gcgtgccggg ctggcggatg cgatttgcga tctggtttcc
      481 accggtgcca cgctggaagc taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa
      541 gcctgcctga tccagcgcga tggcgaaatg gaagaatcta aacagcaact gatcgacaag
      601 ctactgaccc gtattcaggg tgtgatccag gcgcgcgaat caaaatacat catgatgcac
      661 gcaccgaccg aacgcctgga tgaagtcatc gccctgctgc caggtgccga acgcccaacc
      721 attctgccgc tggcgggtga tcaacagcgc gtagcgatgc acatggtgag tagcgaaacc
      781 ctgttctggg aaacgatgga aaaactgaaa gcactgggtg ccagttcaat cctggtcctg
      841 ccgattgaga agatgatgga gtgatcgcca tgagctttaa cacaatcatt gactggaata
      901 gctgtactgc ggagcaacaa cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa
      961 gcattacccg caccgttaac gatattctcg ataacgtgaa aacgcgtggc gatgaggccc
     1021 tgcgtgaata cagcgcgaag tttgataaaa ccacggttac cgcgctgaag gtgtctgctg
     1081 atgagatcgc cgccgccagc gaacgcctga gcgacgagct aaaacaggcg atggcggtgg
     1141 cagtaaagag tattgaaacc ttccacactg cgcaaaaact gccgccggta gatgtagaaa
     1201 cgcagctagg cgtgcgttgc cagcaggtca cgcgcccggt ggcttcagtg ggtttgtata
     1261 ttccgggcgg ctccgccccg ctcttctcaa cggtattaat gctggcaact ccggcgcgta
     1321 ttgcgggctg taaaaaagtg gtgctgtgct caccgccgcc gattgccgat gaaattcttt
     1381 atgcggcgca gctgtgcgat gtgcaggacg tgtttaacgt cggcggcgca caggccattg
     1441 ccgcgctggc gtttggtacg gaatctgtgc cgaaagtgga caaaatcttc gggccgggta
     1501 acgcctttgt caccgaagca aaacgccagg taagccagcg tctggacggt gcggcgatcg
     1561 atatgcccgc aggcccgtcg gaagtgctgg tgattgctga cagcggcgct acgccggatt
     1621 ttgtggcttc tgatttgctt tctcaggctg aacacggccc tgactcacag gtaattttac
     1681 tgacccccga tgctgatatg gcgcgtcgcg ttgccgaggc cgtcgaacgc caactggcag
     1741 aactgccgcg tgccgaaacc acccgccagg cactgaacgc cagccgcctg atcgtgacta
     1801 aagatttagc gcagtgcgtg gagatctcca accagtacgg cccggagcac ctgatcattc
     1861 agacccgcaa cgcccgcgaa ctggtcgatg gcatcaccag cgccggttcg gtatttcttg
     1921 gtgactggtc accggaatcg gcaggcgact acgcctccgg caccaaccac gtgctgccga
     1981 cttacggtta caccgccacc tgttccagcc tcgggctggc agatttccag aaacgcatga
     2041 ccgtacagga actgtcgaaa gaaggtttct ccgcgctggc ttcgaccatt gaaacactgg
     2101 ccgccgccga gcgcctgacc gcccacaaaa atgccgttac tttgcgtgtt aacgtcctta
     2161 aggagcaagc atgagcaccg tgactattac tgatttagcg cgtgaaaacg tccgcaacct
     2221 gacaccgtat cagtcggcgc gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa
     2281 cgaatatccc acagccgtgg agtttaagct tactcagcaa acgctcaacc gctacccgga
     2341 atgccagcca aaagcggtga ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca
     2401 ggtgctggtc agccgtggcg cggacgaagg tattgaactg ctgatccgcg ctttttgcga
     2461 accgggtaaa gacgccatcc tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc
     2521 tgaaaccatt ggtgtcgaat gccgcacagt gccgacgctg gaaaactggc aactggactt
     2581 acagggcatt tccgacaagc tggacggcgt aaaagtggtc tatgtttgca gccccaacaa
     2641 ccccaccggg caactgatca atccgcagga ttttcgcacc ctgctggagc taacccgcgg
     2701 taaggcgatt gtggttgccg atgaagctta tatcgagttt tgcccgcagg catcgttagc
     2761 tggctggctg gcggaatatc cgcacctggc gattttgcgt accctttcga aagccttcgc
     2821 gttggcgggg cttcgttgcg gatttacgct ggcaaacgaa gaagtcatca acctgctgat
     2881 gaaagtgatc cccccctacc cgctctcgac gccggttgcc gacattgccg cacaggcgtt
     2941 aagcccgcag ggaatcgtcg ccatgagcga acgggtggcg caaattatta cagaacgcga
     3001 atacctgatt gccgcactga aagagatccc ctgcgtggag caggttttcg actctgaaac
     3061 caactacatt ctggcgcgct ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca
     3121 gggcattatc ttacgtgatc agaataaaca accctcttta agcggctgcc tgcgaattac
     3181 cgtcggaacc cgtgaagaaa gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg
     3241 atgagtcaga agtatctttt tatcgatcgc gatggaaccc tgattagcga accgccgagt
     3301 gattttcagg tggaccgttt tgacaaactc gcctttgaac cgggcgtgat cccggaattg
     3361 ctgaagctgc aaaaagcggg ctacaagctg gtgatgatca ctaatcagga tggtctggga
     3421 acacaaagtt tcccgcaagc ggattttgat ggcccgcaca acctgatgat gcagatcttc
     3481 acctcgcaag gcgtgcagtt tgatgaagtg ctgatttgtc cgcacctgcc cgccgatgag
     3541 tgcgactgcc gtaagccgaa agtaaaactg gtggagggtt atctggctga gcaagcgatg
     3601 gatcgtgcca acagttatgt aattggcgat cgcgcgaccg acattcaact ggcggaaaac
     3661 atgggcatta atggtttacg ctacgaccgc gaaaccctga actggccaat gattggcgag
     3721 caactcacta aacgagaccg ttacgcccat gtagtgcgca acaccaaaga gacgcagatt
     3781 gacgtccagg tgtggctgga tcgcgaaggt ggcagcaaga ttaacaccgg cgttggcttc
     3841 tttgatcaca tgctggatca gatcgccacc cacggcggtt tccgcatgga aatcaatgtc
     3901 aaaggcgacc tctatatcga cgatcaccac accgtcgaag ataccggcct ggcgctgggc
     3961 gaagcgttaa aaattgccct cggcgacaaa cgcggtattt gccgctttgg ttttgtgctg
     4021 ccgatggacg aatgccttgc ccgctgcgca ctggatatct ctggtcgccc gcacctggaa
     4081 tataaagccg agtttaccta ccagcgtgtg ggcgatctca gcactgaaat gatcgaacac
     4141 ttcttccgtt cgctctctta caccatgggc gtgacactac acctgaaaac caaaggtaaa
     4201 aacgatcatc accgtgtaga aagcctgttc aaagcctttg gtcgtaccct gcgccaggcc
     4261 atccgcgtcg aaggcgacac cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga
     4321 tccttgatac tggctgcgcc aacctgaact ctgtgaagtc tgccattgcg cgccacggtt
     4381 atgaacccaa agtcagccgt gacccggaca ttgtgttgct ggccgataaa ctgtttttac
     4441 ccggcgtcgg caccgcacaa gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc
     4501 tcatcaaagc ctgtacccaa ccggtgctgg gcatctgctt agggatgcaa ctcctgggac
     4561 gacgcagcga agagagcaat ggcgtcgact tgctgggcat catcgacgaa gacgtgccga
     4621 aaatgaccga ctttggtctg ccactgccac atatgggctg gaaccgcgtt tatccgcagg
     4681 caggcaaccg cctgtttcag gggattgaag acggcgcgta cttttacttt gttcacagct
     4741 acgcaatgcc agtcaatccg tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg
     4801 cggcggtaca aaaagataac ttctacggcg tgcagttcca cccggagcgt tctggtgccg
     4861 ctggcgctaa gttgctgaaa aacttcctgg agatgtgatg attattccgg cattagattt
     4921 aatcgacggc accgtggtgc gtctccatca gggcgattac ggcaaacaac gcgattacgg
     4981 taacgacccg ctgccgcgct tacaggatta cgctgcgcaa ggtgccgaag tgttgcacct
     5041 ggtggatctg accggggcaa aagatccggc taagcgtcaa atcccgctga ttaaaaccct
     5101 ggtcgcgggc gttaacgtcc cggtgcaggt tggcggcggt gttcgtagtg aagaagacgt
     5161 ggcggcgtta ctggaagctg gcgttgcgcg cgtggtggtc ggttccaccg cagtgaaatc
     5221 accggacgta gtgaaaggct ggtttgaacg tttcggtgcc gatgcgctgg tgctggcact
     5281 ggatgtccgt attgacgagc aaggcaacaa gcaagtggca gtcagcggct ggcaagagaa
     5341 ctcgggcgtt tcactggaac aactggtgga aacctatctg cccgtcggcc tgaaacatgt
     5401 gttgtgtacc gatatctcgc gcgacggcac gctggcggga tctaacgtct ctttatatga
     5461 agaagtgtgt gccagatatc cgcaagtggc gtttcagtcc tccggcggca ttggcgacat
     5521 taacgatgtt gctgccctac gtggcactgg cgtgcgcggc gtaatagttg gtcgtgcatt
     5581 actggaaggt aaattcaccg tgaaggaggc catcgcatgc tggcaaaacg cataatcccg
     5641 tgtctcgacg ttcgtgatgg tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc
     5701 attggcgata tcgtgccact ggcaaaacgc tacgccgaag aaggtgcaga tgaactggtg
     5761 ttctacgata tcaccgcttc cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc
     5821 gtggcggaag tgatcgacat tccgttttgt gtggcgggtg ggattaagtc tctggaagat
     5881 gccgcgaaaa ttctttcctt tggcgcggat aaaatttcca tcaactcccc tgcgctggca
     5941 gacccaacat taattactcg cctggccgat cgctttggcg tgcagtgtat tgtggttggt
     6001 attgatacct ggtacgacgc cgaaaccggt aaatatcatg taaatcaata taccggcgat
     6061 gaaagccgca cccgcgtcac tcaatgggaa acgctcgact gggtagagga ggtgcaaaaa
     6121 cgcggcgccg gagaaatcgt cctcaacatg atgaatcagg acggcgtgtg taacggttac
     6181 gacctcaagc agctgaaaaa agtgcgtgaa gtttgccacg tcccgctgat tgcctccggt
     6241 ggcgcgggca ccatggaaca cttcctggaa gccttccgcg atgccgacgt tgacggtgcg
     6301 ctggcagctt ccgtattcca caaacaaata atcaatattg gcgaattaaa agcgtacctg
     6361 gcaacacagg gcgtggagat caggatatgt taacagaaca acaacgtcgc gaactggact
     6421 gggaaaaaac cgacggtctg atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt
     6481 taatgctggg ctacatgaac ccggaagcct tagacaaaac catcgaaagc ggtaaagtca
     6541 ccttcttctc gcgcactaaa cagcgactgt ggatcaaagg cgaaacctcg ggcaattttc
     6601 tcaatgtggt aagtattgcc ccggactgcg acaacgacac attactggtg ctggcgaatc
     6661 ccatcgggcc aacctgccac aaaggcacca gtagctgctt cggcaacacc gctcaccagt
     6721 ggctgttcct gtatcaactg gaacaactgc tcgccgaacg taaatatgcc gacccggaaa
     6781 cctcctacac cgccaaactg tatgccagcg gcactaaacg cattgcgcag aaagtgggcg
     6841 aagaaggcgt ggaaaccgca ctggcagcaa cggtacatga tcgctttgag ctgaccaacg
     6901 aggcatctga cttgatgtat cacctgctgg tgctgttgca ggatcaggat ctggatttaa
     6961 cgacagtaat tgagaacctg cataaacggc atcagtgagt tacgggctga gcaggtgtga
     7021 tgttgttgtc acacccggag ccattgcccg atgcgcaaac ttatcgtgcc tacacattat
     7081 ctcaacacac gtaggccgga taagatgcgc cagcatcgca tccggcaaaa aaaacgggca
     7141 tggtgtcacc accctgcccg tttctcttaa atgcacaata atattacttc gcgttgtaat
     7201 tacgcagagc gttacgcccc aacacaatcc ccgcgccaac catgccgccc agaagcactg
     7261 ccagaatcaa ggtgattgcc tttttcggac tatcgcgacg aataggtaac gtcggtttca
     7321 tcacatagcg gtaagcatga atatcaagat catcaaactt taatttttca atatccaaca
     7381 ggttttgacg tgtctgatag tagtttggtg agaacaccaa cggacgagtc gcttcatgct
     7441 taatcatcga ctccagcgct tcgctcccta gaaggaacaa cgtatcttgc gtcacatctt
     7501 cagtctgctg aacctgtggc tttgtcacct gcgcctgatt cgcatactgc aacgcttcct
     7561 gaatctgacg gatacgcaga tctttctgct cctgcgcgac cacttcctgg gttcttaaag
     7621 agtcctgcaa gtttttccgt cccagagcaa tgttgtcctt gagatccttt tctagctctt
     7681 gattcacttt atcatcaact tgctgaatgt attgggccaa cttcatttgt gcgccctctg
     7741 cagtttgccc aacataagaa acagtcaatg gtaattgctg gttcttaaca gaaggttcga
     7801 tggtaagttt ttctggctct tcctgattat ccagcgtttc tgctaatgca gagaaggcag
     7861 aactgaagcg accaattaac gtctcctgca aatccgatac tttcggtgca gcctgaccat
     7921 agataacatt catggcattg ttatagccag caatttgccc cacgtcgggc tgagtgataa
     7981 ttgctgttga cgtccatttc tccttcgcta ctgccaaata accaatagcc agggcaatag
     8041 ccacaatgac ggaaatgata attgtcatct tgccacgcca caactgcact agtaaatcaa
     8101 tcaaatcaat ctgttccggg tcatggtttt gcccagaaac attattattt tctactctca
     8161 tcattaccct aactgacgga aatttccaag tccgcatagt ttagcgataa tccccgtgaa
     8221 tgctatagga taagtgataa aaataatgaa ttattgcgga ggtaacaaaa gacaatcagg
     8281 gcgtaaatag ccctgataac aagatgttag tcgctgccaa agagatcgcg ggtgtagacc
     8341 ttatctgcca catccttaag ctcttctgcc atacggttgg agataatgac gtcggcttgt
     8401 tgtttgaagg tggcgagatc acgttccagg cgagagttga agaatgagtc ttctttcatc
     8461 accggctcgt agatgatcac ttcaacgcct ttcgccttga tacgcttcat aatcccctga
     8521 atagaagacg cacggaagtt atctgacccg ctcttcataa tcagacgata aatacccacc
     8581 acttgcggtt tgcatcgaca attgccgaga tcaggttatt cggcacagac tggtagttcg
     8641 ccagtaactg cttggtatct ttcggcagac aataaccacc ataaccaaac gacggattgt
     8701 tgtaatggtt gccaatacgc ggatcgagac aaacgccttc gataatctgg cgagtattca
     8761 gacctaaact ttctgcataa ctatccagtt cattaaagta tgctacgcgc atcgccagat
     8821 aggtattagc gaaaagttta atcgcttctg cttcagtgga gtcggtaaac agggtcggga
     8881 tattttgctt aatcgcgcct tcctgtaaca acgcagcaaa acgttctgcg cgttctgaac
     8941 gctcaccgat gacaatacgt gacggatgga gattatcgta aagggcttta ccttcgcgga
     9001 ggaattccgg agagaaaata atattttcag tacgatattt cttatgcatc gctgcggtaa
     9061 aaccaacggg aaccgttgat ttgatgacca taaccgcata aggatttatc tcaactacgt
     9121 ctttaattac tgattctaca ctggatgtat tgaaataatt agttttagga tcatagtcgg
     9181 ttggagtggc gatgatgaca taatcagcat cccggtaggc ttcattttta tctaatgtgg
     9241 cattaaagtg tattttatct gattgcaaaa actgctgaat ttccttatca acaataggag
     9301 atatccgatc attcagcata gcaacgcgtg acggtaaaat atctaatgcc acaacctcat
     9361 gattttgtgc gattagaagc ccgtttgaca agcctacata gccagtaccg gaaatggtga
     9421 ttttcatttc atgctctcag aattaactta actgtgaatc atgatgtttt tagcatcgtg
     9481 ataagatgct aaaagtttta acgctacgtt gtcagatggt caacgcaacg aaaaacaatt
     9541 cagattttct ttataagaat agctgatgtt tattaagtta atattaagca gtgaaattta
     9601 gtctataaga tatttggcaa aaaaaagccc ggtgcaatat acgccgggcc tcaattttat
     9661 tgttggttaa atcagattaa tccagccatt cagtatggaa cacaccttct ttatcaatgc
     9721 gcttataagt atgcgccccg aaatagtcac gctgtgcctg gatcaggttc gcaggcagaa
     9781 ccgcggcacg gtagctatcg taataggcaa ccgcagcggc gaaggtcgga accgggatac
     9841 cgttctgtac tgcataagcg acgacatcgc gcagcgcctg ctggtagtca tcggcaattt
     9901 gcttgaagta cggggccagc agcaggttag cgatctgcgg attttcggca taagcatcgg
     9961 tgattttctg caggaactgc gcacggatga tgcagccagc acggaaaatc ttcgcgattt
    10021 cgccgtagtt cagatcccaa ttgtattctt cagacgcagc acgcagctga gagaagccct
    10081 gagcgtaaga aacgattttg cccagataca gcgcactgcg aactttttcg atgaactcag
    10141 ccttgtcgcc tgctgactga gcttgcggac cagagagaac tttagatgcg gcaacgcgct
    10201 gatctttcag agaagagata taacgtgcaa acacagactc ggtaatcagc gacagcggtt
    10261 cgccgagatc cagcgcgctc tggctggtcc atttaccggt gcctttgttt gctgcttcat
    10321 ccagaatcac atcaaccagg tagttaccgt cttcatcttt tttggtgaag atatctttag
    10381 tgatgtcgat caggtagctg ctaagttcac cgttattcca ctcggtgaaa gtctgggcca
    10441 gttcttcatt agatagattc aggccacctt tgagcagaga atacgcttca gcaatgagct
    10501 gcatatctcc gtattcaata ccattgtgaa ccatcttcac ataatggcct gcgccgtcgg
    10561 caccaatata agtaacgcat ggctcgccgt cttctgccac ggcagcgatt ttagtcagga
    10621 taggcgcaac cagctcataa gcctctttct gcccgccagg cataatagaa ggacctttca
    10681 gcgcgccctc ttcaccgccg gaaacaccgg taccgatgaa gttaaagccc tctgctgaaa
    10741 gctcacgatt acgacgaatg gtgtcctgga agaaggtatt accaccatca atgatgatgt
    10801 cacctttatc gagatatggt ttcagggaat caatcgcagc atccgtgcct gcacctgctt
    10861 tcaccattaa caggatgcga cgaggcgttt ccagagattc aacaaactct ttcaccgtat
    10921 agtaaggagc cagtttcttg cctggatttt cagcaatcac ttcttccgtc ttttcgcggg
    10981 agcggttgaa aatagagacg gtataaccac gactttcgat gttgagcgca aggttgcgcc
    11041 ccatcactgc cataccgact acgccgatct gttgctttga cattgtttac tcctgtcttc
    11101 tatattatta accgtctatt aaccgttata ttattaaccg tcgtgatttt catcttctaa
    11161 tcaaggaata catatggtta cagcatgctt cttactttat agcgataagt atgccattgg
    11221 gtgtatccat aagaactttg aattttggat acttttctgg attaaaatta gcatagcttc
    11281 gtttagatat aacaataaac gaactattaa aagatttcat cttctctatt aaatcatcct
    11341 gtgggattac ctcacactcg ggattactaa tagtccaatc actatatctt gctaatccaa
    11401 agacgtcacc aattgccatc cctttataga agtaaatacc atcctccaag cctaattgga
    11461 taagattatc tccatataac tcactatatt cacttgcttt cttcatcagt tcatagctat
    11521 agcgactttc taagcgtgaa ttaaaattca agtattcaga ataaaaatat ttgaattcaa
    11581 aatacaacga tcgccccgaa aaaaaataat acacacaagg ataaaatatc gtcccaactt
    11641 gtaatttgta atgttgatat cttttattaa acttgcaatt gagatcgcaa cctgcaatac
    11701 tgacaatggg taaatgggtg ataaatacct gtcaccttgc gtaacagaaa accaaaataa
    11761 gacatatgtt aaaacaatcg agaaaaatac ccaaagtatt ttattattcc taagtgtaaa
    11821 gacagacagt atagcataaa aaacaacatc actgccaact ttattaattg cttgttgcaa
    11881 atctaatgtg gcaggactta taccatgtcg agcttgttct gctgtttgaa cttgtagatc
    11941 aatttcattc cataggtagt gaccaaatat atttcctcca gcaggactaa aaggatcacc
    12001 agagatccaa aaactacgga cataccaata aatgccacca agaattgttg ccaagctaaa
    12061 aataagaaat gtctgtatgt ttctcttttt cgctatcaat ataacaaata tgatggctcc
    12121 atatatggca ccaaatagtt ttacgccaca agcaattcca ataaaaaaac cacataaaaa
    12181 aattaattca ttttgtgttt tattgttttc tgagcgaata tatataagaa tactggctgc
    12241 agcaaaacaa agatagcgtt aaatcaatat atgcataacc aagatgatct ttaagtggat
    12301 tgatagtaaa tagtaggatt gtagcaataa tgcctggata ataagttttc gtaatttccc
    12361 atgaacagcc aacaagtcca caggctatta caaaaaaaca gatatttgct aaaaactgag
    12421 ctaaccttac atctcctaac tgtaaaccta aagccattaa aagatctgca ttttgcggga
    12481 acagaggaaa acgcaaatat tgttcaacaa caattgcatg gtgctcaact aatgaacgag
    12541 ccaaaggtaa gtgatacata gtatcatccc aatcaccggg agctcgcagg gacgataaca
    12601 atgtaaccac aaaaatcaaa acaagaaata aaataacaaa cagcgtactg aactcaatat
    12661 tatttattgc cagccatcta aattgctgac gtttaataat gcttaaaacc caacacaccg
    12721 taaaaattaa aaagatagca atcttattaa attgcccgac catgcccaga aagtaaatta
    12781 tcaaaatatt taaagacatc cctatgcata ggcaataaaa aataaagaaa atttgttaag
    12841 tcctgccacc ccctccttaa aaaaagatga caatacaata ttaccaatga caaaaaatgt
    12901 cattccaaaa gcaaaacaat acacaacata caaatacaac ggcaattcaa tcatagtaaa
    12961 atactctatt tacattatca gtttatagtt tttttcagaa agataaaata atgccttcat
    13021 ttcacgtcta gttgttgtat ttgaatgcaa aataatgcca catacaaatg ccattattga
    13081 cgataacatg atggaggcag ttaaaaaggc agtcggaaat cttctcacca aacctgtatc
    13141 aatataatca acaatgatgg gcaatcctaa aattatagac aaagcagcaa gtaaaacgcc
    13201 gataatacta aaaaagtata gtggccgttc aagcgagtaa agtttaatta ttgttttcag
    13261 aatcttgaac ccatcactcc aagttgatag tttgctcaca ctcccttcag atcgctcgcc
    13321 atattttgta ttaacctcac catacttcat acgtaattct aaagcatgaa ttgtaagctc
    13381 agtttcgatt tcaaatcccc ttgatagaca tggaaaagat ttaacatagc gtctggaaaa
    13441 tacacgatac cccgacaaca tatcagtaaa attcccttta aagatagtct gaacagtacc
    13501 agtcaacaat ttatttcctc agcgatgccc ttttctgtat gtattttgat ctccattctc
    13561 ttgacgacat cctacaacca tatccaattg attttttatg agatggttaa tcatttctgg
    13621 agcagaactt gcatcataag tgtcatctcc atcaaccatt agataaatat ccgcatctac
    13681 atctgagaac atacgtctta cgacattccc cttgcctttc agaggaacag agtgaactgt
    13741 tgctccagca tcctctgcca cttgtgctgt actgtcagtc gaattattat caaatacata
    13801 tatagaagca gttggaatat tcgttttgaa atcatttatg acttttgcta ttgccttctg
    13861 ctcattataa caaggaataa ttacagcgat attaaaagag tgatacataa gcccctcata
    13921 taaactattt agctaatgtg caagtaattg tgtttttaac aaatcgttta ctaacgaaaa
    13981 atgaaatatt ttgatgcgca atatcctaaa accaaactaa aaagagaaaa ggtgaacaat
    14041 gtaaatattg gagataaagc taagagatca aataaaacgc cgaataaata actcatcgcc
    14101 cccataaaaa atataaatat aaaatatcta gccttggttg cagatacttt aaatgagcat
    14161 cttgcatttg caaaaaaaga aaaggttgct gcacatataa aaccaacaat gttacttcgg
    14221 ccttgaccaa aagctaaatt attataacac gcataaaata ccccccaatg taaagcagta
    14281 ttgacaattc caattaaaaa ataactaaaa aaagaactgg tcaataactt ccctattttt
    14341 aacatccttt attttgctcc agaagtgagg ttattactaa tttggatatt ttctatagaa
    14401 aatacccata aaactacaac gcctataata aaaattccca tgggtgtaaa aagaaatgga
    14461 ttagttatag aaacggtaat actgaaaaaa agtattaacg acatcattaa tgatgttttt
    14521 gtactattta aaaaatagcc tttcaaaaag ataagcaaca taggaactaa tgatataaat
    14581 acaccaataa cgcctgtttt ctgaagtatc tcaagaggta ctatttcaat ccttaatcga
    14641 tctaatataa atgaaccaaa tcctcttcca aacaagaacg tcgctaaatc aacattttta
    14701 taataaaaat ataaatcatt aaatctaaca gagtcagaat cttcactgcg tgatccgacc
    14761 atgtaaagca taattataaa tagaacgctg cccaatatta ttataatagt atttaaatat
    14821 agcttatgta atttaaaact ggctaataac agtgataatg ttgtaaaaag taatacacct
    14881 cttgattctg tcaataatat cgcaagcact attatgagac attttaaata ttttttgtcg
    14941 acaaaactga taatcgcacc gacaccaaaa aagtagaagg atttggaaaa aaaagcccca
    15001 tcgggacgaa aaaagaactc gctagtcaat gataagtgtt cataaattaa attaaaattt
    15061 aacaaaccga aattcaacaa aattatgtat gaaatatata acagtgatcc atataatgca
    15121 aatgcattaa ctattttaat aaaaatctca catgaaaata cttttaaagt atatcttagt
    15181 gtaaatagat atataagtga cagcataaaa aggtatggtt gcacatcaaa cagtatatca
    15241 tttattttat taccagctaa taagccgaca aagaagccat aaaaaggaaa taaaagcaac
    15301 catataaaaa gactgacaca tttttgggtg attgaaaaat tgaatgtaac taaattaaaa
    15361 attaaaagta taaaactaaa tacaaataat aaatatcgca aagggaatat tcccaatggc
    15421 tccagtaatc ttccaccacc accaataatc agttcaatac ataaaaatgt tataaaaatt
    15481 ttatttatat tattcatatt cgtaaggtga tgttttttaa aatatgttta aaatcaacat
    15541 taataaaata taaattttat cagccttttt ccgccttaac ttccaaaaac ttaaagccaa
    15601 gatcaaattg cctatataac ctttacccaa ttgattattc acaaagctat catcagctaa
    15661 ggcattagct attttagtta cttcttttcg ataccacttg tttctcacca aaccaagccg
    15721 tttgtatttt gctttaaaac taatgtttgc tcccacttgg ttgttttcat gctgtctgta
    15781 cagcattgtt ggttttctat caatatacca cgaataattt ctcgtccgcg caaatgaata
    15841 taaaaaccaa tcatgcagac agatatcttg tgcagcattt ttattattta tgatgaactt
    15901 cttaaattca ataagcgttt ctttttttat aacatatgta caccccggac ctgctgcttc
    15961 aaaaaaataa tcgaattgag tttgcgactg agccttatca actaaatttt ttcgccctga
    16021 tggataatat gcaattacat tgctggaata acaattcgca ttattaaata ccaagcagtc
    16081 aattgcattt tttattttat ctttatacca aatatcgtcc tgatcactaa aacagatata
    16141 atcatagttt tcaagatcga catctttaat taatctataa aaatttttac ctgccccccc
    16201 aaacttattg acgcttggaa gtatatttat aattgaggga tatgataatt gaaattcttc
    16261 acaaatattt aatgtattat cagttgataa atcatctgat ataaaaattg agatgtctac
    16321 atctttttgt tcaataatac tttttaattg ttcctcaatc cagcattcgc cattatacgt
    16381 agccataagt acagctactc ttttttgatc agaactatgc attataacga ccgcccccag
    16441 taattcctct tattccatcc agcgtagcca aaatatgttt tttcatatat tcaattttat
    16501 ttttttgagt tgcgataatt aaagtcgtat gaattaaaga aaaaacaact tccctaatag
    16561 agagtaaaag aggcacatga ttttttctaa gcaaaagaaa tgcatttctt actcgataat
    16621 aacgacgtac tggcgagtga attggtatgt taatcccaca gatttttact gacttatccc
    16681 caatagcatg aaccatttct atatcaggaa tgatatgaac taatatgcca tagtttaagc
    16741 atctaagaca ccattctgta tcgacataat caataaaaag ttcctcatcc atcattccaa
    16801 caatctcaaa aacttctttt gaaaccatgg ttccggatga gattgttact gaacttttaa
    16861 acggtgtctg tccttcagtg acatgaattt tctctcgaag accatttttt ttgatattac
    16921 aaataggata ataatgtgac ttatctctat caataaaaac tgggccagct atttttgcat
    16981 tttcattatc catatagcag gaaagttttt tgataaactc attgcgaata cggctatctt
    17041 gatcaaaaaa aattatcttc tctgcccctt tttcaagaga atatcgaatc ccaatatttt
    17101 gtgcttttgc tataccttca ttcctaccaa gacaaattaa atgaactttt tcaatgtctg
    17161 ctaatttatc tttcaaggtg aaattagtcc tattatcgac tactacaacg taacaatttt
    17221 gctcctttag ttcagtgatc agtgcattca gatttttaag ttcgggatta tatgtcacaa
    17281 tgacagcgta aatattacta ttcattatcc aagtgactca gtaattggtt aattttagtt
    17341 ttcatataca gaacacattc tagtataaag ccattgtaat aacagagcat ctaaaattac
    17401 acgtatgctc caggatattg cggcacccag taacccatat ttttcgacca taaaatacaa
    17461 aagggctaaa taaggaacca attcagcaca atgaatcaaa gcagtaattt tagattttcc
    17521 taatgcctgg attgcagaga atggtatcat tgccagcgca ttgaagaaaa aaccgatcaa
    17581 taacacattt aatatattaa aggaattaat ttgataattc tcaccaaacc atattttcaa
    17641 tacaagccct gaatataaca agcctatgat tatcaccggc aaacagatta gaaacattaa
    17701 aagcaatgat ttatttacat tacgtttaaa ttctttaaag tctttgatgt tacttaacct
    17761 tggaaacact gccctcccga ttgctgcagg tattattcct aattttagaa taacctccgc
    17821 tggtgcagaa taaaatgcaa ctttatctgc ccccataata tttgacacta taaatcgatc
    17881 aaaatacacc atgaccggac ttatgatatt acttactgtc atccaaccgc caaagaaaaa
    17941 gagtcgttta aaggttttat aacaaaaatg aacccccgaa attttaatat cattcctgac
    18001 ataataggca gagactaaaa tcgaaataac tcttgcaaat attaaaccag caaccgctgc
    18061 cgacaatgta gcactataaa aaacaaatat ggccggaatt cccgcgatgc aagagcttga
    18121 tatagatttt tgaatattta caatgccaaa tttttcatcc ccctcaagaa tggctgacca
    18181 taattgattt agaataaata atggaatgca aatagccaac agtttaaatg ctagttgtat
    18241 atcactatgt tcaacaccag aaatttttag ataattaaca attccatcag agaaaatcaa
    18301 taataaaaaa gcaccaaagc atgaaaatag caccaaaaaa gatgtacttg ttgaaattac
    18361 cttggttctt tcatgatgat tatcacgatg aattgcaatt tctctaataa cagagcgagt
    18421 caggcctaca tcaaaaattc cagcataccc aacaagtgca attgcaattg tataaacacc
    18481 gaaaccttca ggccccaaac ctcgagcgag aaatcctagc gaggggatag caattaaagt
    18541 tggtattgca tagccaaaaa ggttccagac actatttttt attatgctca ttattacatt
    18601 tctttatcat ataaaataat cagcatctac aaatttttga ccattaatat ctttatcaga
    18661 aagcaatgga ttttgaaccg gccattctat atttatactt ttgtcgctcc ataaaatccc
    18721 acgctcttgc atttttgaat agaaattatt tgttttgtag acaaattctg cttcatcact
    18781 aagaacgcaa aacccatgag cgaatccttc cggtatccat aattgacgct tattctccgc
    18841 cgacaaattt acaccaaccc acttgccaaa tgttggtgat gatttccgaa tatcaacagc
    18901 tacgtcaaaa acttcaccga caacacagcg taccagtttt ccttgagcat aaggttccag
    18961 ctgatagtgc agtccgcgta acacaccctt agttgattta gaatggttat cctgaacaaa
    19021 ctcaaccttt cgtcctacag cctcttcgaa aactttctga ttgaagcttt ccataaaaaa
    19081 gccccgctca tcaccaaata cttttggttc aaaaattagc acatcaggaa tttcagtttt
    19141 aattacgttc attttattaa taacctttaa tcattttcag cagatactga ccataagcat
    19201 ttttcttcag cggctcggct aataccttca cttgctcagc atcaataaag cctttacggt
    19261 gagcaatttc ttccgggcag gaaactttca gcccctgacg ctcttcaatt gttgcaatga
    19321 agttgcttgc ttcaataaga ctttgatgtg tccccgtgtc cagccatgca taaccacgcc
    19381 ccatcatagc gacagacaaa cgtccttggt ccatataaat acggttaata tcggtaattt
    19441 ccagttcgcc acgtgcagat ggtttaaggt ttttcgccat ttctacaacg tcattgtcat
    19501 agaaataaag cccagtaacc gcatagttac tttttggttc ctgcggtttt tcttccagac
    19561 taattgccgt accgttatca tcaaattcca ccacaccgta gcgctcagga tcatttacgt
    19621 gataggcaaa aaccgttgcg ccactctctc tgttaacagc ggtatccatt aatttcggca
    19681 ggtcgtgacc atagaagata ttatcaccaa gtaccagcgc acaatcatca ccacctataa
    19741 attcttcacc aatgataaat gcttgcgcca gaccatccgg actcggttgt actttatatt
    19801 gcagattaag cccccactgg cttccatcac caagcaattg ttgaaaacgt ggtgtatcct
    19861 gcggcgtact gataatcaga atatctcgaa tacccgccaa cataagcgtt gaaagcggat
    19921 aataaatcat cggtttatca taaatcggta gcagttgttt gctcacagcc attgtcacag
    19981 gataaagacg agtgccggaa ccaccagcca gaataatacc tttacgcgtt ttcatttcac
    20041 cattcctttt aattcatctc gctctagcat catgagcgag atgcaataat ttgttaaatt
    20101 gccgtggtcg taaataattc gttgagcata cgtttcacgc ccacctgcca gtcaggcaag
    20161 acaagcgcaa agttctgctg aaatttttcg gtattgaggc gagaattatg gggacgacgt
    20221 gctggtgtgg gataggccgt tgtcggcacg gcattaagtt tgttaagagc aaggttgatc
    20281 cccgctctgc gcgcttcttc aaataccaga gcggcataat cgtgccaggt tgttgtacca
    20341 ccagcaacca gatggtacaa accagcaact tctggtttat ttgcggccac gcgaatggca
    20401 tgagcggtgc aatcagccag taattcagcc cccgttggtg cgccgaattg atcgtttatt
    20461 acagccaact cttcgcgttc ttttgccagg cgcaacatcg ttttggcaaa gttatttcct
    20521 ttaccagcgt atacccagct ggtacggaaa ataagatgct ttgcgcaatg ttcttgtaac
    20581 gctttttccc cagctagctt ggtttcaccg tagacattta gcggtgctgt tgcatccgtt
    20641 tccagccatg gcgtgtcacc gttgcctggg aaaacataat cagtggagta gtgtataacc
    20701 caggctccga cttcattagc tgcttttgca atcgcttcga cgcatgtcgc gttaagtaat
    20761 tgcgcgaaat tcggttctga ttccgctttg tctactgcag tgtgagcagc agcattaaca
    20821 ataacatcgg ggcgaatttt tttgacggtt tcagccacac cttcggggtt gctgaaatct
    20881 ccacaataat cagtggagtg aacatcgagc gcaatcaagt tacccagagg agccagagca
    20941 cgctgcagtt cccaacccac ctgccctgtt ttgccgaaaa gcaggatatt cattactgac
    21001 ggccctcata gttttgttca atccacgact gataagtacc gcttttcaca ttctcaaccc
    21061 aatttgtatt agccagatac cattccaccg ttttacgaat cccgctctca aacgtttctt
    21121 gcggtttcca gcccaattcg cggctaattt tatcggcatc aatggcgtaa cggcggtcat
    21181 gccccgggcg atcggcaaca taagtaattt gctcacgata agatttctct ttcggtacaa
    21241 tctcatccaa caaatcacaa atagtgagca ctacgtcgat gtttttcttt tcgttatgtc
    21301 caccaatgtt ataagtttca cccgctttac cttcggttac gacggtatat aacgcacgag
    21361 catgatcctc tacatacaac cagtcgcgaa tttgatcccc tttaccataa ataggtaatg
    21421 ccttaccttc cagagcattc agaataacca atggaataag tttttccggg aagtgataag
    21481 gaccataatt gttcgagcaa ttagtcacaa tggttggtaa accataagtc cgcttccagg
    21541 cacgtaccaa atgatcgctg gaagctttag acgcagaata cgggctactt ggcgcgtaag
    21601 ctgtcgtttc cgtaaatagc ggtaacgctt cgttattatt tgcttcatcc ggatggggta
    21661 agtcaccata aacttcatca gtagaaatat gatgaaagcg gaaacttttt ttcttttcat
    21721 cattcagagc agaccagtaa ttgcgcgccg cttctaaaag aacataagtc cccacaatat
    21781 tggtttcaat aaatgccgca gggcccgtta ttgaacggtc aacgtggctc tctgccgcca
    21841 ggtgcatcac cgcgtctggc tggtgctgtg cgaaaatacg cgacatggct acagcatcgc
    21901 agatatctgc atgctcaaaa gcataacgtt cagaatcaga aacatcagcg agtgattcca
    21961 ggttcccggc gtatgttaat ttatcgacat taacaacact atcttgcgta ttatttatta
    22021 tgtgacgaac aacagcagaa ccaataaacc ctgcgccacc agtaacaagt atcttcactt
    22081 ttttattcca tatagctata gagcatgctg tgaaatagac tgctccagat ttgattaata
    22141 gaggtattaa tacacgctac cgcccctggc ttaacagcta ccagtgcact gcatgcatct
    22201 caacgatgtg gcgagcgtta gccactcgcg ccaaatccga aaaattcaaa cgttaattgt
    22261 cttaccaaac agccgtagaa acaaggaaaa tcctggaaaa atttgaataa tatgagctat
    22321 gtaacttctt gtttttatag gctttataaa aacggcaacg gcaggaaaat tcaatgagca
    22381 aaacttatgc cacgaatctt cactgccgtt ataattttct tatcaaccgt tacatccggt
    22441 cagattttca ttattcgctt aacagcttct caataccttt acggaacttc gccccttctt
    22501 tcaggttgcg taggccatac ttcacaaacg cctgcatata gcccattttt ttgccgcagt
    22561 cgtaactgtc gccggtcatc agcattgcat caacggattg ttttttcgcc agctcggcaa
    22621 tagcatcagt cagctgaata cgtccccatg caccaggctg agtacgttcc agttccggcc
    22681 aaatatcggc agaaagcaca tagcgaccta cggccatgat gtctgagtcc agcgtctgcg
    22741 gctgatccgg tttttcgata aattcaacaa tgcggctgac tttaccctca cggtccagcg
    22801 gctctttagt ctggatgacg gagtattcag agaggtcacc cggcatacgt tttgccagca
    22861 cctggctgcg gcccgtttcg ttgaaacgtg caatcatggc agcaaggttg taacgtagcg
    22921 ggtcggcgct ggcatcgtcg atcacaacgt ctggcagtac cacgacaaat gggttgtcac
    22981 caatggcagg tcgcgcacac aaaatggagt ggcctaaacc taaaggttcg ccctgacgca
    23041 cgttcataat gatcacgccc ggcggacaga tggactgtac ttccgccagc agttgacgct
    23101 tcacgcgctg ctcaaggagt gattctaact cataagaggt gtcgaagtgg ttttcgaccg
    23161 cgttcttgga cgcgtgagtt accaggagga tttctttgat ccctgcagcc acaatctcgt
    23221 caacaatgta ctgaatcatt ggcttgtcga cgattggtag catctctttg ggtatcgcct
    23281 tagtggcagg caacatatgc atcccgagac ccgctacagg aataactgct tttaaattcg
    23341 tcattatttc atccacctgt aaaatggttg ctgaattata gcttgttcga tttttttcgc
    23401 cagcatcaat taccctgaat tgattactga attacttgtg atgttacgcc gcttcgttgt
    23461 ggattgcagt agcattgttc ctaagtatgg ctccattttt ccaggaatgg tcgcaaatct
    23521 actccctccg ttccggcaat ctaaagttaa tcttttccac attaacaata tggtgattaa
    23581 tcctgtcgat atcgacggag ctttgccctc tttcattcac cgcatgaaca tttgcaagag
    23641 acagcagtgt ttcttttttc gccataaaga cgccgcgaac gtctttgcgc atgtcgaagt
    23701 tcatgctcaa tgctggtcca actgaggatt cctgcatcac attgatattt cgcataaaaa
    23761 gatgttgcgg cttgttgtgt aactccagcg aggcacgctt catctcaatg ttagttagcg
    23821 acacaaagga gacggcattc ccggcggaga tttggatgcc gcgcaattta taagcaagat
    23881 gggtattatc cagttgaata ttattcactc ggaaattttg cggtatcgag agatatttgc
    23941 ctttaattac cccatagccg attaacatcc cggcgctatt aatcatttca atattatcaa
    24001 tcacgaaatt gtcacaaccg taaatagcga ctgtcgcgtt atcaatgccc gctttcttac
    24061 tgaaatccgg cgtgatattg cgggcattga tattacgaat aacaaaatgt ttaccatttt
    24121 caacatggat caactgccga caatccgatc ccgtgatatt cgccacgaca aagtttttca
    24181 ctgcctggtc ttccgggtag ttgttatcat aagtgcttcc cgcaaggcct atgccgatgc
    24241 cccagttgat tttgccgttg gtacagttga tacgctcgat gacatggtca gatatcaaaa
    24301 tatcactgtc gttaattgcc acgttccatt caatggcgtc gccctgtaag tcgctgaact
    24361 tacagttggt aatgttggca ccgataatct ggttatgaaa tccctggcgt aagatggcgt
    24421 aattagcgtg gctgacagtg aggttatcga tggtcaggtt gcgcatgacc cgtttgtttt
    24481 tgccgccaat ataaatctgc attaccgggc caaagccgct catcgccagc cctttgatgg
    24541 tgcagtcaga accacgcaca tccagggtaa tgttatgcat accgccgccc tctccccctg
    24601 tcacctggct gccgtcctgt aagacaaatc gccctctgcc gttgccgcgc aggcttccaa
    24661 ggatgtgtaa cgttttgccg ggagggatga agatgccggt gttgatattg tcacaaacga
    24721 atccggcagg cacgacgacc gtttgccctt cgctgaaggc ttgtttaaat gaggcgatcc
    24781 agtcatgtgg gttgtagtcg ttaatgttaa cgctttgtcg ggcgggaagt gcgcgagcga
    24841 aaggggtatg gaggaaggca agcgccgagc ttgccgtcag gaaggtgcgt cgggagagtg
    24901 ttttaaatgg catacgttct cctctataaa gcctgcagca agctggcgag ttctcgatta
    24961 atcacctgct ggttaaaatc gtgttcgact ttttcgcacg cacgtttgac gaccggagcc
    25021 agttcgtcgg tatccagttg gctaaacgcc gccagacgtt gtgccagtgc gcgagcatcg
    25081 ttctcaggca gcagccagcc ggatttatcg gcctccacca gttcaggaat tccgctatgc
    25141 agagtagaaa ccaccggaat accgaccgcc atcgcttcca ttagcgccac cggaatgcct
    25201 tccatatcgc catccgcgcc cgttaccgat ggcaacagga agacatccgc gtcgtcgagc
    25261 atctccttca cttcatggct cggtttaaag cccggcatct ctaccacatc ttccagttga
    25321 tattgttcga tgatggtgcg caggcgtcgt tcccacaggc caatgccgag gatgcgatag
    25381 cgaaatgcca cgccctgctc tttcaactga cggcaggctt caatcgccac atgcaggcct
    25441 tttttctcgg ttaagcgtgc gacagatatg atttccagcg gcgttgcggg cgctttcacc
    25501 ggacgcgggc taaagcgcgt catgtccacg cccatacgcg atacggcgat tttttctctg
    25561 gggcagccca ttttttgcag ccttccggcc cacagatcgc ttatcggtaa catcaggtcg
    25621 ccacggcgaa acagttgttg atattcggga gtgtagtggt tgagcacttc ccgactggag
    25681 atatcaatac cgtggaagat ggtggcaatt ttgccgcgaa tgatacccag ttcgcgtagt
    25741 tttgctgcgg ttacccccgc cgggccaaaa tgagcgatga acacatcggc gcgaaacggt
    25801 gttgcgacct gaccacaaat ggcagacaaa atcaggttcc gcgactcggc accatagcgt
    25861 ttgaggttga gcgcatgcca ggtattttta cgatgaatgc cacgtaaggt ctggctggcg
    25921 cggtggcgca gtttcgctac tttgcccgca ggttcgtcct gtaaccagcg ggttctggcg
    25981 acaaggttgt atttcgtcca tgccgcgtgg gtgttctctg tatcgccttt ttgcaaagcg
    26041 acaatctcca cttcaaagcc catatcaata aacgcggtaa tttgattgag gacgaaggtt
    26101 tccgacgaca gcgggaattt caataagaag aagccgacct tcatttcacc tccccgatgc
    26161 gctcaagcac agactgcacc atctgcattc ccgtctgacg ttcgcgactg acggcttcgt
    26221 taaatcgagc gttcagcacc ggaagctggc ctaaggtatc cgcaaccatc gcttgcaggc
    26281 tgccgtccag taaatgacga atatcaatcg ccatctctgg cagtcccagc tgctgcataa
    26341 tcccggcgga tttatgttca tagttgatgg caatcgccgg agtagcaaag ttcattgaga
    26401 taatggcgga gtgcaggcgc gtaccgacgg tgagttcaca ggctccgaga attttgccca
    26461 tttccagatc gttgagttca tccatcacta cgtggtaacg ggcaggatcg ctgatgtgct
    26521 ggcgcagatt gagcgccacc atgcggtcgt ctttgttata gctgtcgata cccgtacagg
    26581 tggagagcgc aatcacctga tagccttcat cgagaatgcg attgaccacc ccggcaaagg
    26641 ctttttcata cgcttgttga gtggtgccga gacgtttgtc gaacggtgcc agttcgcgca
    26701 gggtaattgc cactgttttc tgttgtgcgg caacgtccag ccagtgttga acggcatagc
    26761 tggcggtgaa gtcttcggtg tggtgatcga ccagccacgc ggtatcgacg ccatgttcca
    26821 cttttgcggt ggtgatattt ctgcgtttca tcaaatcaag gctgaccgat tcgcgcagga
    26881 tcagcgcgtc gcagtgacca aagacatagt tcgccagttg gttaaattgc tcatcctgga
    26941 aggggccgac gctgtgacca atcataaaca gcggcttttt cgccataaat gtgcaaagcg
    27001 catgttcaaa ctgcggcacg ccgtagagat cgacaaaaaa cgatccgccg acctgaataa
    27061 tggcgtcgta acctgacagc aggcgcacga agtcggtgaa tccctgggcg atggcgatat
    27121 tgcgcagctt gccagtgtcg gtgacgcgtg agagcaatac ctgatgctgg tagcggcgac
    27181 ggaggacttt tttaatgcgc ccgacaacgc ccgccgcgct attgtgttgt gtcatctgca
    27241 ggaacagcgg atcgcccatt accgggcggt tgagcagcca ggaagaactg accggatagc
    27301 ggctcatcac gtccacttcg gtatgtggat tgaggatgtt gatggcatca agtaagccgc
    27361 gcaagatggc gctgtcgcca cgattgccgc aagtgtggtt gcccagaata agtaatttca
    27421 ttttttcctc ataaatttga tgccaggtga ggctgtgttt gttgtgttcg ttgccgtttt
    27481 ttgcctgatg cgacgctggc gcgtcttatc aggcctacag gtcccgagca cagaaccgta
    27541 ggacggataa ggcgttttac gccgcatccg gcaaccgttg tcggaaccga aaacagcaat
    27601 tcaccccgcc cgtaaaagca ttttcatttt ttcgctgcga caaaactgac gcttcacttc
    27661 caccaccagc ggatggcgtg acagcacaat catcaccaca aacgccagca cgcccgcggc
    27721 tatttgcacc gccagcagca tccccagcgc cagttgccct ttcagcacaa tgcccagcgc
    27781 ataactgacc accagcgtcg gcaacgagag ataaaacggc agccacaaac tcaggatgta
    27841 ctggcgataa ctggaaccaa gaaccggttt aatcatcacg aagtaactca gaatggtgtt
    27901 gataatctgc accagcagga aaccgagcgt gacgccgatt gcgcccgcca tctgcccacc
    27961 aataacaatc gccggaataa acagaaatgt tttgaatacg ttgaatttaa agctgatatc
    28021 gacccgcgct ttcgccatca gcagcgaacc aatcgggtta cccacggagc gcagcagacc
    28081 cacaatacac agcaattgca gcaccggaat aatgctgttc cacttttcgc cgaataccag
    28141 cggtacgaag ttattcgaca ccaccatcag ccccagcagc gccggaaagt tgataatccc
    28201 cactatcgac agcagcttgt agaagttaac acgcagcttt tcggtgtcgt cctgaatttt
    28261 ggcaaacgcc ggaaacaaca cgcgggtgat gattgggttc agcttcatcg gcggtacgac
    28321 ggccacgttg tacgccaggt tgtatccccc tgccacgccc gcgccaagaa tacgcgccag
    28381 caccagcgtt gaaaggttgg tgttgagata gttgatgatg ctgtccgccg tcagccaggc
    28441 accaaaacgt aagttcggtg ccacagacgc cagcgagaag tgcagaccgg ggcaataaat
    28501 tttgcgtcca aagtagccaa acagcagtgt tctcaccgca ctattgacca gataaccgag
    28561 gatcgcggtc atcgccagcg gccagaaatg ggcgctaact accgtaaaag tgaagcccgc
    28621 cagcaccgcg ctggtttcga tcatgccgat tttgttgaac tccagctcct tctgcatcaa
    28681 cgcgcggaac tgttgcccgt gggggatcac cacaaacgcc agcgacaatg ttttaatcaa
    28741 cggtgccagg tccgggttat tcagcacgtc gccgatgaca tcactcaaca aaaacaccgc
    28801 cacacatacc acaagcccca gcccgacgtt cagccagtac agcgtggtga gttcaaggtg
    28861 actgatttct tttcgctgaa taatcgagtt agcgataccg aagtcagaaa gcgtatctgc
    28921 cagcgcgata atcaccagcg acacagtcag caggccgaac tggtggttgt cgataatccg
    28981 cgccagcacg gtcatctgca ccagcccgag gccgatgatg atcaccgtgg caatagccga
    29041 ccacttcgcg ccgctgatgg ttttttcacg taagctcata tcaatatgcc gctttgttaa
    29101 cgaagccctt gaataccgtc aggaaaacga ttttgatatc gaaccagacg ctccattcgc
    29161 ggatgtactc aaggtcgaac tcgacgcgtt tttccatttt ctccagcgtg tcggtttcgc
    29221 cgcgccagcc attaatctgc gcccagccgg taatgcccgg tttcacttta tggcgcagca
    29281 tgtagccttc aatgagctgg cgatactgtt cgttatgcgc tactgcgtgc ggacgtggac
    29341 cgacaatcga catccccccg gtcagcacat tgataaactg cggcaattcg tccagcgagg
    29401 tgcggcgcag aaagttcccc actttagtga cgcgcggatc gttctgcgtc gcctgggtca
    29461 ccactttgtc gttctccatc actttcatgg aacggaactt ccacactttg atcggcttgc
    29521 catccatacc atagcgagtc tggcggaaaa taaccggccc tggtgaactg agtttcaccg
    29581 ccagcgcaat acagcacagc accggggaga tcagcagcag aataagcgtc gccagcacaa
    29641 tgtcttccgc acgttttagc aggcggttaa cccccgaaag cggcgtgtca tacagcggca
    29701 ccaccggaac gccgttcatc tcttcgagac gtgaatggag aatgttgaag gtaaagacgt
    29761 cggggatcag cagcaccgaa caggtggtat ccgccagttg atggaccagt tttttcactc
    29821 gtgcgccgtc gcacatttgc atcgcgatat agacgttatg aatcttgccc gcttttgcat
    29881 cctcgaccag ctgttgcagg ttacccgccc agtcgttaga aacgccgccc ggtttcgggt
    29941 cgtggtaaac gcccaccact tcaaacccta accacggctg attacggaaa ctctccatca
    30001 gcatttgtcc ggcggctaaa tcccccgcta cggcgaccat ccgcttgttg tagccatgat
    30061 tacgcagcca gcccgcccca atgcgaatac atgaacggca aaccaccagt ccgatgctgg
    30121 tcagcccata ccacgccagc cagattttca gttgcgtgtc gaaatcattg ttgaacgcca
    30181 ccagtccggc gctgaaaatc acgcttaagg tccagttttg tagcaacagg gcaaattctg
    30241 tcgctgcccg aacaccgcgc catgagcgat aaaaatcggt gatgccgccc agcatctgga
    30301 acaccaccag cgtaatcagc gccaccaaca ggtgcatgta gaggaatgac agtccgctga
    30361 cttcgcaaac cagccatagc ccggcaaaca tgatggtgat atctgaaaag cgttgcacca
    30421 tagagattaa cgatgcattg gttttcgctc gctcgcgctt ttttagattt gtcatcgttg
    30481 ttcctgttat tagcccctta cccgcagtgg ggtaagggaa gatccgacat tactcgttca
    30541 gcaacgtcag cagagttcgc gttcgcgctt ccatcagcgg cacatcaccg cgcgattcca
    30601 cattcaggcg caccaccggt tcggtattgg aggtgcgcag gttaaagcgc cagtcggcaa
    30661 aggtcatgct gatgccatcg ctgcgatcca ccgccagcgc ctcacggcta aaatgctgtt
    30721 ccacgcggtt aatcgcctca acgggttgcg ccagtttgct gttgatctca ccgcttgccg
    30781 gaaacgccgc catccggtcg cgcaccagtt cgcccagcgt ttttcctttc aggcacacca
    30841 gttcggcgac cagcagccac gggatcatgc cgctgtcgca gtaagcgaaa tcacggaagt
    30901 aatggtgggc gctcatttcg ccaccgtaga tggcgtcttc cttgcgcatc cgttctttaa
    30961 taaaggcgtg tccggttttc gacattaccg gcgtgccgcc tgcagcagtc accacatcaa
    31021 tagtgttcca agagagacgc ggatcatgga tgatcttcgc gccgggattt ttttcgagga
    31081 acgcttctgc cagcaggccg acaatgtagt agccctcgat aaactgccct ttttcgtcaa
    31141 acagaaaaca gcggtcaaaa tcgccatcaa aggcaatgcc catatccgcg ccgtgtttga
    31201 tgaccgcatt gcgggtgtcg tcgcgacatt ccggcagcag cgggttagga ataccgttgg
    31261 ggaaattgcc gtccggtgtg ttgtgtactt tgattaattc cacgggcgcg ccgagggctt
    31321 taaagcgggc ttcaatggcg tccaccaccg gacccgctgc gccattcccg gagttgatca
    31381 ccagcttgag cggcgtgaga tttttgacat tgatataacc gaacaggtga tcaacgtaag
    31441 cgtcacgcag gttgatttgc tgatagcgac cgcgtttggt ttcatcgacg ggaggaaagt
    31501 cgttggcttc agccagacgc tggacgtcgc gcagtccggt atcaccgctg atcgtgcgag
    31561 ccccctcgcg caccagcttc atgccgttgt aatccatcgg attatggctg gcagtaactt
    31621 caatgccgcc atccacgccg agatggaacg tggcgaaata gatctcttcg gtgccggaca
    31681 taccgatatc cagcacatcg acgcccgcat cctgtaaacc ttttgccagc gccagtttta
    31741 aggtttcgct ggtgagacgg acatcaccgc ctaacacaat ggttttcggt ttgagaaatt
    31801 cgccataggc gcgaccaatg cgccaggcga tatcttcatt cagttcttcg cctaatttcc
    31861 cgcgaatatc ataggcttta aagcaggtta attttttcat gtcgttaccc tttttcaggc
    31921 aatagttggc cctgaccgaa acggccaatt ttatttttgt cgttatttac accgcggttt
    31981 cgcattcatt gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa ccgaaccgta
    32041 ggtcggataa ggcgttcacg ccgcatccga caaacagcgc ctgatgcgac gtttacaccc
    32101 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32161 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32221 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    32281 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    32341 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    32401 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg cgaccatcgg
    32461 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    32521 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg accaggcctg
    32581 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    32641 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    32701 agcccgcatc catcggcacc acaacagcat ctgccgtagg ttccatgacc gcgtaatcca
    32761 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    32821 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    32881 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    32941 cgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33001 caaaggccac cgtatcctgc tcacccacag acacttcacc gcgacgaata tagccataac
    33061 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33121 gcatggcatt acgcacggcg gcacggaacg cgtcttcatc ggcaatcaca tgatccgccg
    33181 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33241 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    33301 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    33361 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    33421 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    33481 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    33541 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    33601 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    33661 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    33721 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    33781 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    33841 gcccatcttc agcagtgcag gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    33901 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    33961 aatggcaaaa atcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34021 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34081 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34141 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34201 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34261 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    34321 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    34381 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    34441 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    34501 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    34561 ggcggcccct tcttctcgtt tgtagcgcca ggcggaatag ttctcaccca cctgccattg
    34621 cgggtagtaa ggcggtgcgg taatgacccg cacctcatga ccttgtgccg ccaaccattc
    34681 caccatctcg ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt taatgccgta
    34741 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggctcgg
    34801 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    34861 tcatgctgct catccggcag taacagctct tcttccgcta ctctgaagcg aaaaccgagc
    34921 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa atgctgccag
    34981 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    35041 cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    35101 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcgccgcg actgttctcg
    35161 acaataaagt cgagagagac aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga
    35221 cgtaaaaaca tcattccccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    35281 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    35341 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    35401 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    35461 tgccgacgtt aatgtgcgac agcatcggtt gggtattctc cagccagact tcatgcgcca
    35521 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    35581 gtgtaccgct gccccatacc accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc
    35641 gacgcagcaa tgctggtatc acatgggagt tactcgggtg gaagttgtca tgcggcccgt
    35701 acaggttggt cggcatgaca gtgcggtaat cgcgtccata ctggcggttg taagattcgc
    35761 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    35821 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg
    35881 atccgagaaa catcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    35941 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    36001 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    36061 cggcgcggct gtccagcagg ttcagttcat cgcgggtacg taataccagc tccacatcac
    36121 cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacaatgg ccagcgataa
    36181 aaatgcgttg tttactcatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    36241 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    36301 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    36361 gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    36421 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    36481 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gctgccgcca tttccacaaa
    36541 ctgacgtacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    36601 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    36661 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    36721 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    36781 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    36841 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    36901 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    36961 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    37021 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcctaggt tgtacacttc
    37081 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    37141 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    37201 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    37261 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    37321 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    37381 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    37441 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    37501 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    37561 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    37621 ttatttcacc gagggtatat aaattgacgt catcgccaac ccacgcgtaa tcacctaagg
    37681 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    37741 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    37801 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    37861 accctttcgg caccgagaa
//
LOCUS       DAJTRN010000030        37383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRN010000030 DAJTRN010000000
VERSION     DAJTRN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(15..899)
                     /gene="yhdJ"
                     /locus_tag="OO328_001579"
     CDS             complement(15..899)
                     /gene="yhdJ"
                     /locus_tag="OO328_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5930183.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(985..1281)
                     /gene="fis"
                     /locus_tag="OO328_001580"
     CDS             complement(985..1281)
                     /gene="fis"
                     /locus_tag="OO328_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5930184.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1307..2272)
                     /gene="dusB"
                     /locus_tag="OO328_001581"
     CDS             complement(1307..2272)
                     /gene="dusB"
                     /locus_tag="OO328_001581"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5930185.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2601..3482)
                     /gene="prmA"
                     /locus_tag="OO328_001582"
     CDS             complement(2601..3482)
                     /gene="prmA"
                     /locus_tag="OO328_001582"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5930186.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3494..4945)
                     /gene="panF"
                     /locus_tag="OO328_001583"
     CDS             complement(3494..4945)
                     /gene="panF"
                     /locus_tag="OO328_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5930187.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4935..5177)
                     /locus_tag="OO328_001584"
     CDS             complement(4935..5177)
                     /locus_tag="OO328_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5930188.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5286..6635)
                     /gene="accC"
                     /locus_tag="OO328_001585"
     CDS             complement(5286..6635)
                     /gene="accC"
                     /locus_tag="OO328_001585"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5930189.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6646..7116)
                     /gene="accB"
                     /locus_tag="OO328_001586"
                     /gene_synonym="fabE"
     CDS             complement(6646..7116)
                     /gene="accB"
                     /locus_tag="OO328_001586"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5930190.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8094..9068)
                     /locus_tag="OO328_001587"
     CDS             complement(8094..9068)
                     /locus_tag="OO328_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5930191.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9220..11160
                     /gene="csrD"
                     /locus_tag="OO328_001588"
     CDS             9220..11160
                     /gene="csrD"
                     /locus_tag="OO328_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5930192.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11465..12508
                     /gene="mreB"
                     /locus_tag="OO328_001589"
     CDS             11465..12508
                     /gene="mreB"
                     /locus_tag="OO328_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5930193.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12574..13677
                     /gene="mreC"
                     /locus_tag="OO328_001590"
     CDS             12574..13677
                     /gene="mreC"
                     /locus_tag="OO328_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5930194.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13677..14165
                     /gene="mreD"
                     /locus_tag="OO328_001591"
     CDS             13677..14165
                     /gene="mreD"
                     /locus_tag="OO328_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5930195.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14174..14767
                     /gene="yhdE"
                     /locus_tag="OO328_001592"
     CDS             14174..14767
                     /gene="yhdE"
                     /locus_tag="OO328_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5930196.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14757..16226
                     /gene="rng"
                     /locus_tag="OO328_001593"
     CDS             14757..16226
                     /gene="rng"
                     /locus_tag="OO328_001593"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5930197.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16294..20094
                     /gene="yhdP"
                     /locus_tag="OO328_001594"
     CDS             16294..20094
                     /gene="yhdP"
                     /locus_tag="OO328_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5930198.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20250..21695
                     /gene="tldD"
                     /locus_tag="OO328_001595"
     CDS             20250..21695
                     /gene="tldD"
                     /locus_tag="OO328_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5930199.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21823..22752)
                     /gene="aaeR"
                     /locus_tag="OO328_001596"
     CDS             complement(21823..22752)
                     /gene="aaeR"
                     /locus_tag="OO328_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5930200.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22935..23138
                     /locus_tag="OO328_001597"
     CDS             22935..23138
                     /locus_tag="OO328_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5930201.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23146..24078
                     /gene="aaeA"
                     /locus_tag="OO328_001598"
     CDS             23146..24078
                     /gene="aaeA"
                     /locus_tag="OO328_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5930202.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24084..26051
                     /gene="aaeB"
                     /locus_tag="OO328_001599"
     CDS             24084..26051
                     /gene="aaeB"
                     /locus_tag="OO328_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5930203.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26143..26415
                     /locus_tag="OO328_001600"
     CDS             26143..26415
                     /locus_tag="OO328_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5930204.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26471..26734)
                     /gene="yhcN"
                     /locus_tag="OO328_001601"
     CDS             complement(26471..26734)
                     /gene="yhcN"
                     /locus_tag="OO328_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5930205.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27099..27569)
                     /gene="argR"
                     /locus_tag="OO328_001602"
     CDS             complement(27099..27569)
                     /gene="argR"
                     /locus_tag="OO328_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5930206.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28004..28942
                     /gene="mdh"
                     /locus_tag="OO328_001603"
     CDS             28004..28942
                     /gene="mdh"
                     /locus_tag="OO328_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5930207.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29004..30071)
                     /gene="degS"
                     /locus_tag="OO328_001604"
     CDS             complement(29004..30071)
                     /gene="degS"
                     /locus_tag="OO328_001604"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5930208.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30161..31508)
                     /gene="degQ"
                     /locus_tag="OO328_001605"
                     /pseudo
     CDS             complement(30161..31508)
                     /gene="degQ"
                     /locus_tag="OO328_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31662..32060)
                     /locus_tag="OO328_001606"
     CDS             complement(31662..32060)
                     /locus_tag="OO328_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5930209.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32254..33381
                     /gene="zapE"
                     /locus_tag="OO328_001607"
     CDS             32254..33381
                     /gene="zapE"
                     /locus_tag="OO328_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5930210.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33600..34028
                     /gene="rplM"
                     /locus_tag="OO328_001608"
     CDS             33600..34028
                     /gene="rplM"
                     /locus_tag="OO328_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5930211.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34044..34436
                     /gene="rpsI"
                     /locus_tag="OO328_001609"
     CDS             34044..34436
                     /gene="rpsI"
                     /locus_tag="OO328_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5930212.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34831..35469
                     /gene="sspA"
                     /locus_tag="OO328_001610"
     CDS             34831..35469
                     /gene="sspA"
                     /locus_tag="OO328_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5930213.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35475..35972
                     /gene="sspB"
                     /locus_tag="OO328_001611"
     CDS             35475..35972
                     /gene="sspB"
                     /locus_tag="OO328_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5930214.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36015..37382)
                     /gene="dcuC"
                     /locus_tag="OO328_001612"
     CDS             complement(36015..37382)
                     /gene="dcuC"
                     /locus_tag="OO328_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5930215.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9033 a         9364 c         9855 g         9131 t
ORIGIN      
        1 ggcttacact tcctttactt tgcaatgaga tcggggtcaa cttcgcttaa ccggcttcgt
       61 tttgacaggt tgccggtctt tctttttttc actttcgcca gttcttccgc ggagtaatgc
      121 gacgcgacat ccagccgtcg aagccccatt ttgatgtact cgctgttgat ctcaatacca
      181 atgaattttc gtccgctggc gatggctacg gcaccggtag taaagctacc ggcaaacggg
      241 tcgagaacga tatcgcctgg gttggaagag gcgagaataa tgcgtttcag taaggctttc
      301 ggtttttgcg tcgggtggtt ctcatattca tccatcaaat aacgcacgcg cggaaaatcc
      361 cagacgttac ccggtacttt ttgatgattg tatggctgtg gaggattttt gcgataatcg
      421 atcaacgcgc gctgcgatcc ggttttggct tctaccagaa tagcatcacc gttgaatgtg
      481 tagttctttg cgtctttcac catcatcagg atgggttcgt acatggagcc gtagtgtttt
      541 ttcgcctgta ctccagaact gtcatatgac cagacgatgc gacttttgat ggtaaaaagc
      601 ttgcggcact ggagatcgat aaagggcatg ttttccgtac tgttcataat gtacatgctg
      661 ccctgctttt tcagaacgcg gtggcactct gcaatcactt caaacagcca gtcgataaac
      721 agatcttctt tccaggcttc gatcagacca tcaaaatttt taccgatgtt atacggtggg
      781 tcggcaaaga gcagatcgac gctttcagta ggtagctttt taagttcggc aaaggcatca
      841 ccgtgaataa tggtcttagc ttcattacca aaccgggtcg gttcacatcc tgttctcatg
      901 gtcactccct ttgtgacacc tataaaaaag gcgcttcccc atgccgagta gcgccttttt
      961 aatcaagcat ttagctaacc tgaattagtt catgccgtat tttttcaatt ttttacgcag
     1021 cgtaccacgg ttgatgccca tcatcagcgc agcacgggtc tggttaccac gggtgtattg
     1081 catcaccatg tccaacaggg gctgttctac ttcagccagt accagctcat agaggtcatt
     1141 cacatcctga ccattcagtt gagcaaaata gttcttcagt gcctgtttaa ccgagtcacg
     1201 caggggtttt tgggttacct gatcctgaga gttaacggta gaaacggtca gtacgtcaga
     1261 atttacgcgt tgttcgaaca tagttctgtc agctctttat ttctgtttac gcaaaatttt
     1321 cgaagtatgc ctccaacgcc tccagctgtt cgctggcatc ctcaatggcg ttgaatgtgc
     1381 gccgaaactg gtcatttgga gcgtgttcct ggagatacca ggaaacgtgt ttacgtgcaa
     1441 ttcggtaccc ttttgccgga ccataaaagt catgcagttc ccgaacgtgc gcgcaaagca
     1501 agcgcttaac ctctgccaaa ggcagtgggg gcagcaactc cccagtgtcc agataatgct
     1561 ggatttcccg aaagatccag ggtcttccct gagctgcgcg gcctatcatc agggcatccg
     1621 cccctgtata gtcgagcaca gctctggctt taagcgggtc agtaatgtcg ccattcgcga
     1681 taaccggaat ggaaactttc tgcttaactg cccgaatact gtcgtactca gcttctccat
     1741 tgaacaaaca ggcgcgtgta cggccatgaa tggtcagagc ctgaatgcca cagtcttcag
     1801 ccagttgggc aatctcttcg cagttacggt gttccggtgc ccagccggtg cgaatcttca
     1861 gggtaacagg aacgtccact gcattgacga cctcggtaag gatcgattta acgacatccg
     1921 ggtactgcaa gagggctgaa cctgcgagct tgcgattcac ttttttagcc gggcaaccca
     1981 tattgatatc aataatctgg gcaccgcttt ccacgttaat acgtgctgca tctgccattt
     2041 ctttcggagc gctaccagca atttgcacgg tgcgaatacc gggttcatca atgtgcacca
     2101 tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt agaagacatc atctcggata
     2161 ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct gtaatgccag
     2221 ccatgggcgc tgcgatcagg cgatttctga gctgatattg tccgatgcgc atgagttaag
     2281 aaatgaccat actgtgactg caaggcggcg tatattacgc attttttgca cgagatgaaa
     2341 ggccaaactt tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa atgagcgtgg
     2401 tgcgataatt actcataaaa atcatcatat tagaaaatag tgactaaaat ttacactcaa
     2461 agaaatttga gtaagttctc aatttttctt tatgaatgaa aattttggca cgcaaatttg
     2521 cgtaaataat cggcaaattt acgtgccttt tgtgagcttg ctcgcacttc gccccgcgtc
     2581 accctacggc gatgcgaagg tcaattctta cgaccggtaa tacggcacca ctcttctttt
     2641 tccacgaccg ggtccagtgc gaagctatcg gcataagctt cacaaacgct ctctgcctgg
     2701 cttgccagaa taccggaaag gcccagcaaa ccgcctgaaa ctggcaggac gctgattaac
     2761 ggtgccagtt cacgtaatgg gcctgcaagg atgttagcga ccaccacgtc ggctttcatt
     2821 tcttctggct gatctttcgg taagtagagt tccagacggt cagaaacgcc attacgttcg
     2881 gcgttatcgc ggctggcctg aatcgcctgc ggatcgatat caataccaat ggcttttgct
     2941 gcacccagtt tcagcgccgc gatcgccaga atgccggaac cacagccaaa gtcgatgact
     3001 gttttaccgg ttaaatcgag gctgtcgagc cattgcaggc acagagaggt ggttggatgg
     3061 gtacccgtac caaacgccag ccctggatct aacatcacgt tgacggcgtt ttcgtccggc
     3121 acatcacgcc agctagggca gatccacagt cgttcaccaa agcgcatcgg gtggaaatta
     3181 tccatccatt cgcgctccca gtctttatct tctagttgtt cgattttatg cgcgaagcct
     3241 gcgccgagca gcggatggtt ttccagaatc gccaccacgt cgttcatatc ggtttcagcg
     3301 tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg cagcggctca
     3361 aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc ttccatcagc
     3421 gcatcgctaa gatcttccgc gttcgcgccg gtggtgttca gtttcagttg gatccaaggc
     3481 atggcaaaac tctttattta tcagtagtca aaacggtagc ttgcgggacg gatgtaccga
     3541 aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg atagggtgga
     3601 agcccaggta ctgaatattc agcgtcgcga gtacggcata cagcacgccg ccaacgatca
     3661 tcgcacttag cgcgcctttg gcgttggcgc gttcccagta aagacccagc accagcggcc
     3721 acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt cagccagatg atcatttctg
     3781 gcggcttcca ggcggcaagc agcagcaacg cgccgagaac taacgtaatt accgccgaca
     3841 tccgcttcag acgcgtctcg ttttgcattt gatccggacg gatattcaga tagagatctt
     3901 taatgatcgt agcggaactt tgcagcagtt gggcgttaat tgtcgacatg atcgcagcca
     3961 taggtgcagc cagaaagatc ccggcagcaa aaggtggcag cacttttacc attaacgtcg
     4021 ggatcaccag atctggtacg gtgagatcgg ggatcactgc acgacctaac gctccggcca
     4081 ggtgcatacc gaacatcagg attgcgacta taatcgtacc gatgatgatc ccccggtgta
     4141 ctgctttgct gtctttataa gagatacagc gcaccgcagt atgcggcagg ccaatcacgc
     4201 caaagcacac cagtacccag aacgacgtca taaaggcagg cgacagaata tcgtcagcgc
     4261 cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt ctgtactgcg ttgcttaagc
     4321 cgccagcggc atgtaccacg ccaataagca gcacaacggt gccaatcagc atcacaagcc
     4381 cttgcatggt gtcgttcagc acgctggcgc gaaagccacc aaaggcggta tataacgcaa
     4441 tgctgatacc aaaaatcagc agcccggttt cataaggaat acccgccgcg gtttccagca
     4501 ggcgcgcacc gccgataaac tgcacggtca tcgcaccaac gaacgcaacc agcaaactca
     4561 aactcgccag ccacaccaga agacgactct ggtagcgggc aaacagcata tcgttcagcg
     4621 tcactgcatt gtagcggcgc gcaagaatcg caaacttctt gccgagaata ccgagtgaaa
     4681 gccagacagc aggaagctga atcatcgcca gcaataccca gcccagcccg tatttataag
     4741 cagctcctgg cccgccgata aacgaactgg cactgatata ggtcgcggtg agagtcatcg
     4801 ccagcacaat accgcccata gagcggctgc cgaggaaata ctcattaagg aaggtgccgg
     4861 tgctccgttt acgcatcgca taaaccgaga taccgaacac caccaccaga taggcgacca
     4921 gcggtagaat tacttcaagc tgcatcgtca tcctccagtg ggatatcgcg atagataaat
     4981 ttcaccatcg cccagcacag tccaataaac agtagcggcg tcaggatgca ggccatctca
     5041 aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag gtaagtaagc gtctactaac
     5101 caaactgcca gatacaaaag ggtcagcccc agcgcccagc gggcctcttt atgggcctga
     5161 acaaaacgag tgtccatttt ttgtccctga tgggtgaaga aagcgaggat tgtaccttat
     5221 gggggttgtc gatccccagt aataaaaaag gccggaaaat ccggcctttt gacgcttttg
     5281 cagtcttatt tttcctgaag accgagtttt ttctccagat agtggatgtt agtgccacca
     5341 tgctggaagt tctcgtcatt catgatgcgg atctgcagat caacgttggt tttgataccg
     5401 tcgatgatca gctcctgcag cgcattcttc atgcgggcaa tcgccacgtc acggttttca
     5461 ccgtagcaaa tcagcttacc gatcattgag tcatagtacg gcggtacggt gtagcccgcg
     5521 tagatatgag actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg ggtgattttg
     5581 cctgggcttg gcaggaaggt gttcggatct tcggcgttga tacgacattc caccgcatgg
     5641 ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt gaccggcagc gatacgcagc
     5701 tgttctttga tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc tacctgaata
     5761 cgggtgttca tttcgatgaa atagaactcg ccgttttcga acaggaactc gaaagtacct
     5821 gcaccgcgat agccgatatc aacacacgct ttagcgcaac gttcgccgat gtagcgacgc
     5881 agttccgggg taatgcccgg tgctggtgct tcttcgacca ctttctggtg gcggcgctgc
     5941 atggagcagt cacgttccgc cagatagata gcgttgccct gaccgtcagc cagtacctga
     6001 atctcgacgt ggcgaggatt ctccaggtat ttctccatgt aaaccatatc gttgctgaaa
     6061 gcagctttcg cttccgcacg ggtcatggag atggattgtg ccagttcagc gtcgccgcgc
     6121 actacgcgca taccgcgacc gccgccgccg ccggaggctt tgataatcac cggataacca
     6181 atgcgtttag caatggcgcg gtttttatcc atatcgtcgc ccagcgggcc gtcagaacca
     6241 ggtacgcaag gaacgcccgc ttttttcatc gccgcgattg cggatacttt gtcgcccatc
     6301 aggcgaatgg tttctgcttt cgggccgatg aagataaagc cggagcgttc aacctgctcg
     6361 gcaaagttgg cgttctcgga gaggaagccg taacccggat ggattgctac tgcgccggtg
     6421 atttcagcgg cgctgatgat tgccgggatg ttcagataac tttttactga cggagcaggg
     6481 ccaatacaga ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc cgtgctggag
     6541 tgcacagcga cagtcttgat gcccagttct ttacaggcac gaagaatacg caatgcaatc
     6601 tcgccgcggt tggcaataac aattttatcc agcatgttcg cctcgttact cgatgacgac
     6661 cagcggctcg tcaaattcta ccggttgtcc actttcgacc agaattgctt tcacggtacc
     6721 ggatttgtcc gcttcgatct ggttcatcat tttcatggct tcaacgatgc acagggtatc
     6781 gcccacgttg actttctgac ccacttcgat gaacgctttt gcgtccgggc ttggggtgcg
     6841 gtagaaagta ccaaccatcg gggaacgtac gatgtgacca ctgatttccg ctgctgctgg
     6901 cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta gattgagctg gctgctgcat
     6961 cattggtgca gcgtaagctt gttgcatcac agggaaactt gcggcaggag ctgcacggct
     7021 aatgcgtact gactcttcgc cttcagaaat ttccagttcg gagatgcctg attcttcaac
     7081 cagctcgatc agttttttaa tcttacgaat atccatgagt gggttccgta ctctttgttt
     7141 agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg acagctgtat
     7201 gcatagcgat aaattccagc aggccgggac gccggtctat tttgccccag agtgtcaatg
     7261 ttagacttga cggacattgt gcagcgatct gctgctatcc tccggcaaaa aacaaaatat
     7321 accttcgttg cgccaatgtc accttttccg cagcgcaaaa actgcgtcag ggaggacgag
     7381 gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa tactggtctt atcagggaat
     7441 ataatcaaca gctaacatgt aaataacctt caacaccgtg taatttgcaa caagccgcac
     7501 aattcacgaa attagcgcca tcatcgacgg aacttcttat aacgtaaggt gaaaagctga
     7561 aaagccagcc ctgcgaagat gaggggctgc aatgagataa tcttcacaga ccacaaataa
     7621 tgtagggatg ccaggatcgc gacaagatag acgacattac gcagcggttg ctggggatta
     7681 cccaattttc actgcatcgt ctggatagat gtgaatgcta aagcacgcaa aatcaaccaa
     7741 ctgacaattc tcagcattaa ataggttcga gtggttaatt tgtagtcaaa taaaatcaga
     7801 ttattcaccc catggtacca gaaatcagta acaggttaag cgtagtgtcg cccaggcaaa
     7861 gcaccataaa cctaacaggc gacggaggcg tatcaataat ggctgtttag aggagtatca
     7921 atgcgcatca atttgtggtc agtcgaaatc atgactttcc agtttggatg atgttgtaca
     7981 acttcctgac caaggtgttc gacgaaagct gtccggctaa ccgcttttcc cgttaaccga
     8041 ttgaataatg atgtcaaaat gagttccctc tcttattatt ccttgctaaa tggttagtta
     8101 accttcacca gcgtgcgacc ctggatctgg ttattaatga tggcctcggc aaagttcggt
     8161 gcctctgaca gagatatctc ttttgccgcc tgggtataga atgattccgg taaatcggcg
     8221 accagtcgct gccaggcttg tgcgcggcgt tctggtggcg tcattactga atccacccct
     8281 tgcaaacgga cattacgcag aataaatggc atgaccgtgg ttggcagagt aaaaccaccc
     8341 gccagaccac aggccgccac gcagccgccg taattcattt gcgccagcac ttttgccagc
     8401 actttgtcgc caacagtgtc aattgcccca gcccagactt gtttttccag aggacgggat
     8461 tcggcaaact catcacgagg gagaataccg ctggcaccta aacttttcag atattcatgg
     8521 gtactttcgc gaccggaaac ggcaacgacc tgataaccca acttatgcag cagcgccacg
     8581 gcggtactgc cgacgccacc actggcaccc gtcacgacaa tctcaccgtc ctgcgggcga
     8641 acaccggcat cttccagcgc catcacacac agcatggcgg taaaaccggc agtaccgata
     8701 atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc acctttcact
     8761 cgcgcctgct ccgccagccc accccagtgg ttttcaccaa cgccccagcc agtgagtaac
     8821 acctcctgac cggcatgaaa acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg
     8881 atcccaggaa tcatcggaaa attacggatg atttttccct taccggtaat cgccagcgca
     8941 tctttatagt tcaggctcga ccagtgaaca tcgaccgtga catcgccctc cggcaggcga
     9001 cgttcgtcca gagtctgtac tgatgcgaga gttttgccgt cctgctgttc taaaagtaac
     9061 gcctgcataa gtggtcctca tgtgcatgat ggattggaaa ataatttctg aagactatac
     9121 tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa tctgatttgc
     9181 tagtatgccc gcttcctcac tatcggagtt aacacaagga tgagattaac gacgaaattt
     9241 tcggcctttg ttacgctgct caccgggtta acaatttttg tgactttgct gggctgttcg
     9301 ctaagtttct acaacgccat tcagtataag tttagtcatc gcgttcaggc ggtggcgacg
     9361 gcgattgata cccaccttgt gtcgaatgac ttcagcgcat taaggccaca aattaccgaa
     9421 ttaatggtgt cagcagatat cgttcgtgta gacctgctgc atggtgataa acaggtttat
     9481 accctggcca gaaatggtag ttatcgtccg gttggcacca gcgatctgtt tcgtgaactg
     9541 agcgttccgt tgataaagca tccggggatg tcgttgcgtc tggtttatca ggatccgatg
     9601 ggcaactatt tccattcgtt gatgaccacc gcgccgctca cgggggcgat tggctttatc
     9661 attcttatgc tctttctggc ggtacgctgg ttacaacggc aacttgccgg gcaagaattg
     9721 ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc
     9781 atctatgaat ggccgcccag aaccagcagt gcgctggata cgctgcttcg tgaaattcag
     9841 aacgcacgcg aacaacacag ccgtcttgat acgctgatcc gctcttatgc cgcacaggac
     9901 gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc agttagcaac gttactggaa
     9961 gatcaggaga aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat
    10021 atgttgagcg atacctgggg gcacagccag gttgaagaac agttcttcac tctgacgaat
    10081 ctgctgtcga catttatgat gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt
    10141 gattttgctg cgctgttacc gcaccggacg ttaaaagagg cagagagcat agccagtcag
    10201 ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga cgatatgatc
    10261 cacattggta tctgtgcctg gcgtagtggt caggataccg agcaggtaat ggaacatgca
    10321 gagtctgcca cgcgtaatgc gggattgcag ggtggcaata gctgggctat ttatgatgac
    10381 tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc
    10441 agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat
    10501 catcgcgaac tcatgtgccg catcttcgat ggtaatgaag aggttagctc ggcggagtat
    10561 atgccgatgg tcttgcagtt tggcttatcg gaagagtatg accgtctgca aatcagccgt
    10621 cttattccac tattgcgtta ctggccagag gaaaatctgg cgattcaggt taccgttgag
    10681 tcgctgattc gtccgcgttt tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa
    10741 tcacaacgaa aacgcataat tattgaactt gcagaggccg atgtaggtca acatatcagt
    10801 cgtttacaac ctgttattcg tttagtgaat gctttagggg tacgggtagc cgtcaaccag
    10861 gctggtttga cgctggtaag caccagttgg atcaaagaac ttaatgttga gttactcaag
    10921 ctccatccgg ggctggtcag gaacattgag aagcgaacgg agaaccagct gctggttcaa
    10981 agcctggtgg aagcctgctc cgggaccagc acccacgttt acgccaccgg cgtgcgttcg
    11041 cgaagcgagt ggcagaccct gattcagcgc ggtgttacag gcgggcaagg ggattttttc
    11101 gcgtcctcac agccacttga tactaacgtg aaaaaatatt cacaaagata ctcggtttaa
    11161 cctgccgttt aatccgtttt cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct
    11221 tgtctgctcg ccagattgtt gcagcacata tgcagatgaa tgaccttacg cggttgcaaa
    11281 caggcgagga atgctgctga tgcattaagc ctttctggac tcaggcagag atttgtaaca
    11341 aaggaaacga actgcactaa ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct
    11401 gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc cagctttcag gattatccct
    11461 tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg acctgggtac
    11521 tgcgaatacc ctcatttatg taaaaggaca aggcatcgta ttgaatgagc cttccgtggt
    11581 ggccattcgt caggatcgtg ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc
    11641 gaagcagatg ctgggccgta cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg
    11701 cgttatcgcc gacttcttcg tgactgaaaa aatgctccag cacttcatca aacaagtgca
    11761 cagcaacagc tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg ttggcgcgac
    11821 ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt
    11881 cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac
    11941 cggttctatg gtggttgata tcggtggtgg taccactgaa gttgctgtta tctccttgaa
    12001 cggcgtggtt tactcctctt ccgtgcgcat tggtggtgat cgtttcgacg aagctatcat
    12061 caactatgtg cgtcgtaatt acggttctct gatcggtgaa gccaccgcag aacgtatcaa
    12121 gcacgaaatc ggttcggctt acccgggcga tgaagtccgt gaaatcgaag ttcgtggccg
    12181 taacctggca gaaggtgttc cacgcggttt taccctgaac tccaacgaaa tcctcgaagc
    12241 actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg aacagtgccc
    12301 gccggaactg gcttccgaca tctccgagcg cggcatggtg ctcaccggtg gtggcgcact
    12361 gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc attccagtcg ttgttgctga
    12421 agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca
    12481 cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc aggggaagcg tctgtttacc
    12541 ctgcctggtc tgatacgaga atacgcataa cttatgaagc caatttttag ccgtggcccg
    12601 tcgctacaga ttcgccttat tctggcggtg ctggtggcgc tcggcattat tattgccgac
    12661 agccgcctgg ggacgttcag tcaaatccgt acttatatgg ataccgccgt cagtcctttc
    12721 tactttgttt ccaatgctcc tcgtgaattg ctggatggca tatcgcagac gctggcctcg
    12781 cgtgaccaac tagaacttga aaaccgggcg ttacgtcagg aactgttgct gaaaaacagt
    12841 gaactgctga tgcttggaca atacaaacag gagaacgcgc gtctgcgcga gctgctgggt
    12901 tccccgctgc gtcaggatga gcagaaaatg gtgactcagg ttatctccac ggttaacgat
    12961 ccttatagcg atcaagttgt tatcgataaa ggtagcgtta atggcgttta tgaaggccag
    13021 ccggtcatca gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa actgaccagt
    13081 cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat
    13141 atccgcgtaa ttgcagccgg taacggttgt acggatgatt tgcagcttga gcatctgcca
    13201 gcgaatacgg atattcgtgt tggtgatgtg ctggtgacct ccggtctggg cggtcgtttc
    13261 ccggaaggct atccggtcgc ggttgtctct tccgtaaaac tcgataccca gcgcgcttat
    13321 actgtgatcc aggcgcgtcc gactgcagga ctgcaacgtt tgcgttatct gctgctgctg
    13381 tggggcgcgg atcgtaacgg cgctaacccg atgacgccgg aagaggtgca tcgtgttgct
    13441 aatgaacgtc tgatgcagat gatgccgcag gtattgcctt cgccagacgc gatggggcca
    13501 aagttacctg aaccggcaac ggggatcgct cagccgactc cgcagcaacc ggcgacagga
    13561 aatgcagcta ctgcgcctgt tgcgccgaca cagcccgctg ctaatcgctc tccacaaagg
    13621 gctacgccgc cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg
    13681 cgagctatcg tagccaggga cgctgggtaa tctggctctc tttcctcatt gcgctgttgc
    13741 tgcaaatcat gccctggccg gataacctga ttgttttccg gccaaactgg gtgttactta
    13801 tcttgttgta ttggatcctg gccttgcccc atcgcgtaaa tgtgggcaca ggttttgtga
    13861 tgggtgccat actggatctg atcagcggct cgatgcttgg cgtacgcgta ttggcgatga
    13921 gcatcattgc ttacctggtg gcgctgaaat accagctttt ccgcaacctc gcattatggc
    13981 agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt ttctgggcag
    14041 agtttttagt gattaacgtc tctttcagac cggaagtgtt ctggagtagt gtagtcaatg
    14101 gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag tttgcagtgc
    14161 aataaaggtt tctatgactt ctctgtattt agcttccggt tctccgcgtc gtcaggagtt
    14221 acttgcgcaa cttggcgtga cctttgaacg tattgttacg ggcattgagg agcagcgtca
    14281 gccgcaggag agcgcgcagc agtatgttgt gcgtctggca cgcgagaaag cacaggcggg
    14341 tgtcgcgcaa acggcacagg atctcccggt gctgggtgcg gatattatcg ttatcctgaa
    14401 cggagaagtg ctcgagaaac cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt
    14461 atcgggtcag acccatcagg tgatgacggc agtggcgttg gccgacagcc agcacattct
    14521 cgattgcctg gtggtcaccg atgtgacttt cagaacgtta acagacgaag acatcgcggg
    14581 ctatgtcgcc agcggtgaac cgttagataa agcaggtgca tacggtattc aggggctggg
    14641 tggctgtttt gtcaggaaga taaatggcag ctatcacgcc gtagtcggct taccgctggt
    14701 tgaaacgtat gaattattaa gtaattttaa cgcactgcgt gagaaaaggg ataaacatga
    14761 cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat attgatggcg
    14821 gtattctgca ggaaattcat attgaacgtg aggcgcgacg cggaatagta ggcaatatct
    14881 acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta gatattgggc
    14941 tggataaagc cgcgtttctt catgcatccg acatcatgcc gcataccgaa tgtgtggcgg
    15001 gtgaagaaca aaagcaattc acggtgcgcg acatctcgga actggttcgt caggggcaag
    15061 atctgatggt gcaggtggtg aaagatccgc ttggcactaa aggtgcgcgc ctgaccaccg
    15121 atatcacgct gccttctcgc tatctggtat ttatgccagg ggcttctcac gttggggttt
    15181 cccaacgaat tgaaagcgaa tcagaacgtg aacgtctgaa aaaagtggtc gcagagtatt
    15241 gcgacgagca gggcgggttt atcatccgca ccgcagcgga aggggttggc gaggctgaac
    15301 tggcctccga tgccgcttat ctgaaacgcg tctggaccaa agttatggag cgtaaaaagc
    15361 gcccgcagac ccgttatcag ctgtacggcg aactggcgct ggcgcagcgt gttctgcgtg
    15421 attttgccga cgccgaactg gaccgcattc gcgttgactc acgcctgact tacgaagcat
    15481 tgctggagtt tacctcggag tacattcccg agatgaccag caagctggag cattacaccg
    15541 gacgccagcc gattttcgat ctctttgatg tcgaaaacga aatccagcga gcgttggaac
    15601 gcaaagtaga actgaaatcc ggtggttatc tgattatcga ccagaccgaa gcgatgacca
    15661 ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa tctggacgac actattttca
    15721 ataccaatat tgaagcgacg caggctatcg ctcgccagtt acggttgcgt aatctgggcg
    15781 ggattatcat tattgatttc atcgatatga ataatgaaga tcaccgccgc cgcgtgctgc
    15841 attcgctgga gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg
    15901 cgctggggct ggtggagatg acgcgtaaac gcacccgcga aagcattgag cacgtactgt
    15961 gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct
    16021 atgaaatcat gcgcgagatt gttcgtgtcc accatgctta cgactccgac cgtttcctgg
    16081 tctatgcttc tccggcagta gccgaagcct tgaaaggcga agagtcacac tcgctggcgg
    16141 aagtggaaat tttcgttggc aaacaggtta aagtacaaat tgaaccgctc tataaccagg
    16201 agcagtttga cgtcgtaatg atgtaaacag atgctgggcc gccatccggc aaagggtttt
    16261 tgagtcacat ttttagcaga caaggagtga cgggtgaggc gattgccggg gattttactg
    16321 cttactggag ccgcgctcgt tgtgatcgct gccctgctgg ttagcggcct gcgtattgct
    16381 ttaccgcatc ttaacgcctg gcgtccggaa atcctcaaca aaatagaatc cgcgactggc
    16441 atgccggtag aagccagtca gctctcagcc agctggcaga attttggccc gacgcttgaa
    16501 gcacacgaca tccgtgcaga actaaaagat ggcggcgaat tttcggttaa acgcgttact
    16561 ctggcgctgg atgtctggca gagcctgtta catatgcgct ggcagtttcg cgacctcact
    16621 ttctggcagc tgcgctttcg caccaacact cctatcacca gcggtggtag cgatgacagt
    16681 ctggaagcca gtcacatcag cgatctgttt cttcgtcaat ttgaccattt cgatctccgc
    16741 gacagtgaag tcagttttct gacgccatcc ggtcagcgcg ccgagctggc gatcccacaa
    16801 ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag cctctccagc
    16861 cttaccggac agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg
    16921 ttaagcaatg gtcgcgtctg gctacaggcg gatgacatcg acctgaagcc gtggctcggt
    16981 aaatggatgc aggacaatat tgctctggaa acggcacagt tctcccttga aggctggatg
    17041 acgatcgaca aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg cggtgccagc
    17101 tggttgggcg agaagcaaac gcatacgctg tcggtggata atctgaccgc gcatattacg
    17161 cgtgaaaatc cgggctggca gttctctatt cccgatacac ggatcacgat ggacggcaaa
    17221 ccctggccga gcggagcatt gacgctggcc tggataccgg aacaggacgt tggcggcaaa
    17281 gacaataaac gcagtgacga actccggatt cgcgctagta atctggagct ggcaggcctg
    17341 gagggcatac gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt ttggcgctcc
    17401 acacaaccga gcggcaagat taacactctg gcgctggata tcccgcttca ggcggcagac
    17461 aagacccgtt ttcaggcatc gtggagcgat ctggcctgga agcaatggaa attattaccg
    17521 ggtgcggaac acttctccgg gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg
    17581 tcgatgaagc aggcaaagat gccttacgaa acggtattcc gtgcgccact ggaaatcgcc
    17641 gacggccagg caactataag ctggctgaac aatgacaaag gtttccagct ggatgggcgt
    17701 aatattgacg ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta cctgcaacct
    17761 gctaacgatg aaccctggct gggtattctg gctggcatca gtaccgatga tggttcacaa
    17821 gcctggcgct atttcccgga aaacttgatg ggtaaagacc tggttgatta tttaagtggt
    17881 gcgattcagg gcggtgaagc ggataacgcg acgctggttt atggtggcaa tccgcaactc
    17941 ttcccctata aacacaacga aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag
    18001 tttgccttcc agccggactg gcctgcatta actaaccttg atattgaact ggactttatt
    18061 aacgacggtt tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat
    18121 ctcaccgcag tgatccctga ctactcaaaa gaaaaactgc tgattgacgc tgacattaaa
    18181 ggtccgggta aagccgttgg cccttacttt gatgagacac cgctgaaaga ttctctgggt
    18241 gcgaccctgc aagaactcca gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc
    18301 ccgctgaacg gcgaactggt aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg
    18361 tttatcaaac cactcgacag catcctgaaa aatttgagcg gtaaattcag ctttatcaat
    18421 ggcgatctgc aaagtgaacc actgacagca agctggttta atcagccgtt gaacgtggat
    18481 ttttccacca aagaaggggc aaaagcctac caggttgcgg tgaatctcaa cggtaactgg
    18541 caaccggcga aaaccggcgt tctgcctgaa gcggtgaacg aagcattgag tggcagcgtg
    18601 gcgtgggatg gtaaagtggg cattgatctg ccttatcatg ctggcgcgac ctataacgta
    18661 gagctgaacg gcgatctgaa taatgtgagt agtcacttac cttcaccgtt agccaaacct
    18721 gcgggtgaac cactagcggt aaacgttaag gttgatggca atctcaacag ctttgaatta
    18781 accggacagg ctggtgcgga taaccatttc aatagccgct ggttgcttgg tcaaaagctg
    18841 acgctcgatc gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa
    18901 agtggcgttg aacttaatat gccgccgatg aatggtgccg agtggctggc cctgttccag
    18961 aaaggcgctg cggagagtgt cggtggtgca gcgagtttcc cacaacacat aacgttacgt
    19021 acgcctatgt tgtcactggg aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg
    19081 acggcaaatg gcaccctggt tgaggcgcaa gggcgtgaaa tcaacgccac gctagcgatg
    19141 cgtaataacg cgccgtggct ggcgaatatc aaatatcttt attacaaccc gagcgtggcg
    19201 aaaactcgtg gtgattcaac gccgtcatca cctttcccga caacggagcg cattaacttc
    19261 cgtggctggc cggacgcaca aatacgatgc acagagtgct ggttctgggg gcaaaaattc
    19321 ggtcgcattg acagtgatat caccatttct ggcgatacgt taacgctgac caatggactg
    19381 attgatactg gtttctcgcg gcttactgcc gatggtgaat gggttaataa tccggggaat
    19441 gaacgtacct cgctgaaagg aaaactgcgt gggcagaaaa ttgatgccgc cgcagaattt
    19501 tttggtgtca cgacgcccat acgccagtcg tcatttaatg tggattacga tttacactgg
    19561 cgcaaagcac cctggcagcc agatgaagcg acgttgaatg gcatcattca tactcaactg
    19621 ggtaaaggcg aaattaccga aatcaatacc ggacatgccg ggcaattgct gcgcttattg
    19681 agcgtagatg ccctgatgcg taagctgcgt tttgatttca gagacacttt tggcgaaggg
    19741 ttctattttg actccattcg cagcaccgcg tggattaaag acggcgttat gcacaccgac
    19801 gacacgctgg tggatggcct ggaggcggat atcgccatga aagggtcggt aaatctggta
    19861 cgccgcgacc tgaatatgga agcggttgtc gcaccagaga tttctgcgac ggtgggcgtg
    19921 gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag tgtttgccgc cagtaaagtg
    19981 ctggggccgc tgtggagcaa agtctccatt ttgcgctatc acatttcggg tccgctggac
    20041 gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag aaaaagcgca atgatttgac
    20101 gagggcgcgt aattgcccca atctcatatg ataatcgttg ccaaaggcca acgagccgga
    20161 acataactgt aggccggata gggcgttcac gccgcatccg gcagccgtaa aaaatcctct
    20221 actgcagtaa ctaacgagta gcaaaaacga tgagtctgaa cctggtaagt gaacaattgc
    20281 tagcggcgaa cggcctgaaa catcaggact tgttcgcgat cctcggtcaa ctggccgaac
    20341 gtcgccttga ttatggcgat ctctattttc agtcgagcta tcacgaatcc tgggttttag
    20401 aagaccgcat tattaaagat ggttcttaca acatcgatca gggcgttggc gtgcgtgcaa
    20461 tcagcggtga aaaaaccgga tttgcttacg ctgaccaaat cagcctgctg gcgctggaac
    20521 agagtgcgca agcggcgcgc accatcgtcc gtgatagtgg tgatggcaaa gtacagacgc
    20581 tgggcgcggt agagcatagc ccgttgtata cctcggtaga tccgctgcaa agcatgagcc
    20641 gtgaagagaa gctggatatc ctgcgtcgcg tcgataaggt tgctcgcgaa gcggacaagc
    20701 gcgtacagga agtgactgcc agcctcagtg gtgtctatga attaattctg gttgcggcca
    20761 ccgacggcac gctagcggcg gatgtccgtc cgctggtgcg tctttccgtg agcgttctgg
    20821 tcgaagaaga tggcaaacgc gaacgcggtg ccagtggcgg cggcggtcgt tttggttatg
    20881 agttcttcct tgccgatctt gacggcgaag tccgtgcgga tgcatgggca aaagaagcag
    20941 tgcgtatggc gctggtcaat ctttctgccg ttgctgcacc agcgggcacc atgccggtag
    21001 tacttggcgc aggttggccg ggcgtgctgt tgcatgaagc ggtaggtcac ggtctggaag
    21061 gcgacttcaa ccgccgtggc acttcagtat ttagtggaca ggtcggggag ctggtggctt
    21121 cagaactgtg taccgtggtt gatgacggca cgatggtcga tcgccgtggt tcggtggcga
    21181 ttgatgatga aggtacgcca ggccagtaca acgtgctgat tgagaacggc attctgaaag
    21241 gctacatgca ggataaactc aacgcgcgtt tgatggggat gacgccgact ggcaacggtc
    21301 gccgtgaatc ctacgcccat ctgcccatgc cgcgtatgac caacacctat atgctgccgg
    21361 gtaaatcgac cccgcaagaa attattgaat ccgttgagta cggtatctat gcaccgaact
    21421 ttggtggcgg tcaggtggat atcacctccg gcaaattcgt tttctccact tcagaagcat
    21481 atctgattga aaacggtaaa gtaacgaagc cggtgaaagg cgcaacgttg attggttccg
    21541 gtatcgaaac catgcagcag atttcgatgg ttggcaacga cctgaaactg gataacggcg
    21601 tgggtgtctg cggtaaagaa gggcaaagtt tgccggttgg cgtgggccag ccaacgttga
    21661 aagtcgataa cctgactgtt ggcggtactg cgtaataatt tcattatttt cagaaggata
    21721 attaatcttt ctacgtgcac gaacggtccc ctcgctcctc tggggttagg gtgaggggaa
    21781 cccgttggca caggtttgta gaacgtaaca gtacaatatg aattacttct ctttcccgcg
    21841 cccgtgcatc tcctgaaaca atttaccgac ctcaacaaaa taatccgtca gcgagttgat
    21901 cacgacctgt accttcagcg gcagcttatc tttttcggta tataacgcat aaaccgggcg
    21961 tggatctgac tgataacgcg gcagcaggat ctccagctcc ccacgattga tctcgttgat
    22021 cacccacatc agcggcacgt aggcgatccc ggcacccgcc gttagccagc gcaccagcgt
    22081 catcggatca ttagtcacaa atctcccttg cgggatcagg cgagtcgaga tcccttccgg
    22141 tgcaatcagt tcaaattcat tgtcgggccg cacgctgtat tcaagccatg aatggctact
    22201 taaatcggcg ggtttttccg gtatgccgta ttgtgtgaga tagcttttcg cggcgcacac
    22261 caccattggc atcgcgccaa gacggcggga aaacaggctg gaatcctgca acgcgccgac
    22321 gcggatcacc acatccagac cgtcggcaat caggtcgggg gctggaattc cggtaaccag
    22381 attggcgctc aaacctgggt attctttcag cattttggct gtcagcccgg cgagaacatt
    22441 ttgtgccata gttgaagaac agccaatgcg tagcgtcccg atgggggtgt tattgaaggc
    22501 atacagttgc tcatgaacat cctgcacttc atgaagcata cgacggcagc cctggtagta
    22561 aattctaccg gcttcggtca ggccaatgct gcgtgtgcta cggtttaaca gctttacctg
    22621 caactcatct tccagttttg acaccgtctg actgatggac gaaacgctca tctgtagctg
    22681 tctggcggcg gcggtaaaag agccaaattc aactactttg gcaaacaccg acatgcgttt
    22741 tagtcgttcc attattcact ctgacttaaa agtgatttag atcacataat atagataaca
    22801 gcataacagt tacgctaata tattaaatat caatctacag caatgttgct ctcgcccggc
    22861 tttccatgcc cttctctgcg gcgacagatg ctgaaaataa taacgcctgc tctctctata
    22921 caaccaaggt caacatgagt ctgtttcccg ttatcgtggt gtttgggctg tccttcccac
    22981 cgatattttt tgaattgctt ttatcactgg cgattttctg gctggtgcgc cgggtacttg
    23041 tgccaacagg tatctacgac tttgtctggc atccggcgtt gttcaacacc gcgctctatt
    23101 gctgcttgtt ttatttgata tcgcgactgt tcgtttgagg ttgaagtgaa aacactaata
    23161 agaaaattct cccgtacggc catcacggtc gtattagtca ttctggcctt catcgcaatt
    23221 tttaatgcct gggtctatta caccgaatcc ccctggacgc gtgacgcgcg ctttagcgct
    23281 gacgtcgttg cgatcgcgcc ggacgtttct ggactcatta cccaggtgaa tgttcatgat
    23341 aaccagctgg tgaaaaaagg acagatactg ttcaccatcg accagccgcg ctatcaaaag
    23401 gcgcttgagg aagcgcaagc cgatgttgct tattatcagg tactggcaca ggagaaacgc
    23461 caggaggccg gacgtcgtaa ccgtctcggt gtgcaggcga tgtctcgcga agagatcgac
    23521 caggctaaca acgtactaca aacggttctg catcagttag cgaaagcgca ggcgacccgc
    23581 gatctggcaa aactggatct tgaacgcacg gtgatccgag cgccagcaga tggctgggtg
    23641 accaacctca acgtctatac cggtgagttt attactcgag gatcaacggc ggttgcgctg
    23701 gtgaaacaga actccttcta tgtactggcc tatatggaag aaactaagct ggaaggggtg
    23761 cgtccggggt atcgtgcaga gatcacgccg cttggcagta acaaagtgct gaaagggact
    23821 gttgatagtg ttgccgctgg ggtcaccaac gccagcagca cgcgtgacga caaagggatg
    23881 gcgaccatcg actctgacct tgaatgggtg cgtttggcgc aacgtgttcc ggttcgtatt
    23941 cgtctcgaca accagcaaga gaacatctgg cctgcgggca ccactgctac agtggtggtc
    24001 actggcaaac aagatcgcga cgaaagccag gattcgttct tccgtaaaat ggcccatcgc
    24061 ctgcgtgagt ttggttaatc acgatgggta ttttctccat tgctaaccaa catattcgct
    24121 ttgcggtaaa actggcgacc gccattgtac tggcgctgtt tgttggcttt cacttccagc
    24181 tggaaacgcc acgctgggcg gtactgacag cggcgattgt tgccgccggt ccggcctttg
    24241 ctgcgggagg tgaaccgtat tctggcgcta ttcgctatcg tggctttttg cgcatcatcg
    24301 gcacatttat tggctgtatt gccggactgg tgatcatcat tgcgatgatc cgcgcaccat
    24361 tattgatgat tctggtgtgc tgtatctggg ccggtttttg tacctggata tcctcgctgg
    24421 tacgaataga aaactcgtac gcgtgggggc tggccggtta taccgcgctg atcattgtga
    24481 tcaccattca gccggaacca ttgcttacgc cgcagtttgc cgtcgaacgt tgtagcgaga
    24541 tcgttatcgg tattgtgtgt gcgattatgg cggatttgct cttttctccg cgatcgatca
    24601 aacaagaagt agatcgagag ctggaaagtt tgctggtcgc gcaatatcaa ttaatgcaac
    24661 tctgtatcaa gcatggcgat ggtgaagttg tcgataaagc ctggggcgac ctggtgcgac
    24721 gcaccacggc actacaaggc atgcgcagca acctgaatat ggaatcttcc cgctgggcgc
    24781 gggccaatcg acgtttaaaa gcgatcaata cgctatcgct gacgctgatt acccaatcct
    24841 gcgaaactta tcttattcag aatacgcgcc cggaattgat cactgatact ttccgcgaat
    24901 tttttgacac gccggtagaa accgcgcagg acgtccacaa gcagctcaaa cgcctgcgga
    24961 gagttatcgc ctggactggg gaacgggaaa cgcctgtcac catttatagc tgggtcgcgg
    25021 cggcaacgcg ttatcagctt ctcaagcgcg gcgttatcag taacacaaaa atcaacgcca
    25081 ccgaagaaga gatcctgcaa ggcgaaccgg aagtaaaagt agagtcagcc gaacgtcatc
    25141 atgcaatggt taacttctgg cgaaccacac tttcctgcat tctgggcacg cttttctggc
    25201 tgtggacggg ctggacttcc ggcagtggtg caatggtgat gattgcggtc gtgacgtcac
    25261 tggcaatgcg tttgccgaat ccacgcatgg tggcgatcga ctttatctat gggacgctgg
    25321 ccgcgctgcc gttagggctg ctctactttt tggtgattat ccctaatacc caacagagca
    25381 tgttgctgct gtgtattagc ctggcagtgc tgggattctt cctcggtata gaagtacaga
    25441 aacggcgact gggctcgatg ggggcactgg ccagcaccat aaatattatc gtgctggata
    25501 acccgatgac tttccatttc agtcagtttc tcgacagcgc attaggacaa atcgtcggct
    25561 gtgtgctcgc gttcaccgtt attttgctgg tgcgggataa atcgcgcgac aggactggac
    25621 gtgtactgct taatcagttt gtttctgccg ctgtttccgc gatgactacc aatgtggcac
    25681 gtcgtaaaga gaaccacctc ccggcacttt atcagcagct gtttttgctg atgaataagt
    25741 tcccagggga tttgccgaaa tttcgcctgg cgctgacgat gattatcgcg caccagcgcc
    25801 tgcgtgatgc gccgatcccg gttaacgagg atttatcggc gtttcaccga caaatgcgcc
    25861 gcacagcaga ccatgtgata tctgcccgta gcgatgataa acgtcgtcgg tactttggcc
    25921 agttgctgga agaactggaa atctaccagg aaaagctacg catctggcaa gcgccaccgc
    25981 aggtgacgga accggttcat cggctggcgg ggatgctcca taagtatcaa catgcgttga
    26041 ccgatagtta agtcaaaacc gacgccaaaa gcgtcggttt tttcatggct atacttagcg
    26101 atgcacggca gaactcgccg cgaaacgtga cggtggcaac agatgaatat ttatgccttt
    26161 gattttgatg agattgagag tcaggaggat ttttatcgtg actttagcca aacctttggt
    26221 ctggctaaag ataaggtacg cgatctcgac tcactatggg atgtgttaat gaacgatgtc
    26281 ctgccgctac cacttgagat tgaatttgtt catctgggag agaaaacgcg tcgccgtttt
    26341 ggcgcgttaa tattgctgtt tgatgaggca gaggaagagc tggaagggca tttgcgtttt
    26401 aatgttcgtc attagcgcaa aaaaaagccc acgaaccggg ggcaatatcg tcggacaaaa
    26461 cgatgagggt ttatttgtac agttcagccg tagcgtgcca ggtgtcaccg ctacgagctt
    26521 cagtaatctg gtaggccgtt gcgcctttct cttccgcttt tttgttcagc atttcacgta
    26581 tatccattgg cgaagacgcc acaccactta cggatacggt cccgattgct tcacgatttt
    26641 gtgcttgtgc agcatcaatg gagtcggcag cgaatgcacc gaaagagaga acagaaagta
    26701 cgcttaatgc agctacagtg gttttgattt tcatgatttt tacctcgaca taatctttta
    26761 gctgggcctt tgtttcgtga cccatatcac aaaatcaagt atacactaat tattgaacta
    26821 attaatacca cactaactgt ttcagttgat actaagtgtt aaaaatgtga attttaataa
    26881 caaaaaagta taaaaaacac ggttatttat tattaattat agtattttca attagataat
    26941 gaattttgca taatgtgctt tttttaccgt atgtattcat tttgtgaatg atgtgtcgcg
    27001 gtaaaacgca ctattcgtta aatgtatcgc aggagaaaga aggggaatga gagggataag
    27061 caacattttc cccgccgcca gaagcgacgg ggcagagatt aaagctcctg gtcgaacagc
    27121 tctaaaatcg cttcgtacag gtctttgacg gtgaaaccgt tagcaggggt ggtaaagatg
    27181 gtgtcatcgc cagcgatggt gcccagaata ccttctgctt tgcccagtga gtccagcagg
    27241 cgagcaatta actgcgccgc gccagggctg gtatgaatca cgacaactgc atcgttgtag
    27301 tcgatatcca gcaccagatt cttcaatgga ctggaggtgg ttggtacacc cagttcagct
    27361 ggcaggcagt aaaccatttc cattttggca ttgcgtgtac gtacagcacc aaacttggtc
    27421 aacatccgcg agactttaga ctgattaata ttgtcaaagc cttgctcctg caacgcggcg
    27481 acgatttcgc cctgggagct aaatttctct tctttaagta atgctttaaa tgctttaact
    27541 agttcttctt gcttagccga gcttcgcata agtcacccga tatggtggtt gatacaacat
    27601 tattgtgcat acagatgaat ttttatgcaa acagtcagcc ctgaagaagg ctgaaataat
    27661 gttatgaaag agcgggattt tatcaaattt cgttattgag aaacatgcct gcgtcacggc
    27721 atgcaaattc tgtttaaaag taaattaatt gttatcaaat tgatgttgtt ttggctgaac
    27781 ggtagggtat attgtcacca cctgttggaa tgttgcgcta atgcataagc gactgttaat
    27841 tacgtaagtt aggttcctga ttacggcaat taaatgcata aacgctaaac ttgcgtgact
    27901 acacattctt gagatgtggt cattgtaaac ggcaattttg tggattaagg tcgcggcagc
    27961 ggagcaacat atcttagttt atcaatataa taaggagttt aggatgaaag tcgcagtcct
    28021 cggcgctgct ggcggtattg gccaggcgct tgcactactg ttaaaaaccc aactgccttc
    28081 aggttcagaa ctctctctgt atgatatcgc tccagtgact cccggtgtgg ccgtcgatct
    28141 gagccatatc cctactgctg tgaaaatcaa aggtttttct ggtgaagatg cgactccggc
    28201 gctggaaggc gcagatgtcg ttcttatctc tgcaggcgta gcgcgtaaac cgggtatgga
    28261 tcgttccgac ctgtttaacg ttaacgccgg catcgtgaaa aacctggtac agcaagttgc
    28321 gaaaaactgc ccgaaagcgt gcattggtat tatcactaac ccggttaaca ccacagttgc
    28381 aattgctgct gaagtgctga aaaaagccgg tgtttatgac aaaaacaaac tgttcggcgt
    28441 taccacgctg gatatcattc gttccaacac ctttgttgcg gaactgaaag gcaaacagcc
    28501 aggcgaagtt gaagtgccgg ttattggcgg tcactctggt gttaccattc tgccgctgct
    28561 gtcacaggtt cctggcgtta gttttaccga gcaggaagtg gctgatctga ccaaacgtat
    28621 ccagaacgcg ggtactgagg tggttgaagc gaaagccggt ggcgggtctg caaccctgtc
    28681 tatgggccag gcagctgcac gttttggtct gtctctggtt cgtgcactgc agggcgaaca
    28741 aggcgttgtc gaatgtgcct acgttgaagg cgacggtcag tacgcccgtt tcttctctca
    28801 accgctgctg ctgggtaaaa acggcgtgga agagcgtaaa tctatcggta ccctgagcgc
    28861 atttgaacag aacgcgctgg aaggtatgct ggatacgctc aagaaagata tcgccctggg
    28921 caaagagttc gttaataagt aattgattag cgaataataa aaaaccggag cacagactcc
    28981 ggtttttgtt ttgagcactc gacttaattg gttgccggat attcctgaat ggtgacctgc
    29041 agcgttaact gcttatcatc acgcatcact acaaccggga tcaccgaacc agggcgaatt
    29101 tccgccacct gatccatcgt ctccagagca gagatggccg gtttgttatc caccgaaata
    29161 atcagatcgt tgacctgaat acccgcattc gccgctgggc cgtcaggtga cacttcatta
    29221 accacgatcc cttgcagttg atctatacca ccgctctgcg cgtgcagtgg tgcgatctcg
    29281 cgtccgccga taccaatgta gccgcggatc acgcgaccat cgcggatcag cttatccata
    29341 attttggttg ctaactggaa aggaatcgca aagccgatac cttccggcgt ttcgccatcg
    29401 ttactcttat caaacgacag cgtgttaatg cccatcagtt ctcccagcga gttcaccagc
    29461 gcgccgccag agttaccgtg gttaatggaa gcatcggttt gtaggaagtt ttgccgcccg
    29521 gtcgggttca gaccgattcg acccgtggca ctaataatcc cttgggtaat ggtctgcccg
    29581 aggttgtacg ggttaccgat cgccagtact acgtcgccaa tgtgcggtac gcgacgtgca
    29641 ttaattggaa tggtaggtaa accgccagtg gcattaattt taagtaccgc cagatcggtt
    29701 agagagtcag atcccaccag caatgcttca aatacacgtc catcctgtaa ggcgacgatg
    29761 atctgatcgg cgtcgttgat gacgtgttta ttggtgatga tataaccgcg ttgatccatg
    29821 attacaccgg atcccagggt gcggatctca agctggttgt gagagttggt gttcaaacca
    29881 cggttgtaaa cgttaaccac cgctggcgcg gcgcggcgaa ccgccagatt atagctggca
    29941 ggcgtctcat cggtactgtc aaattgcgga gtggaaagcg ggttaaggct gcgcagcgaa
    30001 ggcatggcaa ccagcagaat agcgccgaca attaatccaa tcgcaacgga acgtaagagc
    30061 ttcacaaaca tgatggaggc gtcattaaaa aagggaacgg cagcagcata ccacgagtta
    30121 accggacatc acacgtcagc ctgatgcccg gtttacgaca ttaacgcatc agcagataga
    30181 tgctttcatt gccgcgtaca atttgcaggg cgatgatggc cggttttgcc gccagcactt
    30241 tacgcatttc agcaatcgag ttcacccgat cgcggttgac gccaatgatc acatcgtctt
    30301 tttgcaagcc agcctgagca gctgggcttc ctttgacaac ttcatctatc ttaatacctt
    30361 tgccgccatc ttttagctga ccatcgctca acgttgcacc ttccagcgct ggccgacgaa
    30421 gaggtgctgg tatcaagcgt cacttctact tccagtggtt tgccgttacg cagcaggcca
    30481 agcttcactt tcgtgcccgg ctctgtagtc gcgatacgag agcgcaactc agcaaagcta
    30541 ttcagcggtt tgccgttgag gctggtaata atatcgcccg ctttgacgcc cgctttcgct
    30601 gagccagaac ttggcaacac ttcgctgaca aacgcgccac gctgcacgtc aaggttgaag
    30661 gctttggcga tatcggcact catctcggtg cctttgatgc ctaacaaacc gcgtttgatt
    30721 tcaccaaagt cgataagctg ctgcgccagt gttcgcgcca tattactggg gatggcaaat
    30781 ccaatcccga cgctcccgcc gccaggcgca aggattgcag tgttgatgcc aattaactca
    30841 ccgttaaggt ttaatagtgc accgccggag ttaccgcggt taatggaagc atctgtctgg
    30901 ataaagtttt ccagaccttc aagattcaac ccgctgcggc ctaatgcgga aacaatgcca
    30961 gaggtggcgg tttgcccaag gccaaatggg ttaccgaccg ctacggcaaa atcaccgacg
    31021 cgcaatttat cggagtcggc aatagcgatt tgcgttaatt tgctcgggtt ttgaatttgt
    31081 aacagggcga tatcgctctg gtcatcgcta ccaatcagtt ttgcatcaaa ctcgcgccca
    31141 tcattgagct gaatactgat tttctgtgcc tgattaatca catggttgtt ggtcagcaca
    31201 tagcctttac tggcgttgat gatgacaccg gaacctaaac cttcgaaggg ttgtgcaggt
    31261 tgatccggta aatcatcacc aaaaaacttt ttgaattctt ccgggatttt ctgtccctga
    31321 ctggccgttc cttccacccg tacgctcacc actgccggaa gcactttttc cagcattgga
    31381 gccagactgg ggagaggggc ctgatcggca acctggcctg gaatcgacgc gacggcctga
    31441 aatgacgccg agagagttaa cccggcactt aacgctaatg cactcaacag ctgggtttgt
    31501 tttttcatta ttcctgctct cgtacctgaa tgataagaaa agagattcaa aacagtttga
    31561 tgttattgaa ttttcagacg gctaacaatg agacaccaga ttaaatgata gtcggggagg
    31621 gagagaagag gagggcgcaa tggctgcgcc cgaaaaataa attagtcgcg cttcgcgcca
    31681 gtacgcagca ggccggatgc gccttcagaa tagtcgcgag gcacctgcac cggtgcctga
    31741 tcgttgctgg cttcagactc tgccagacga ttacggaacg ggtttgcttc agcagacagt
    31801 tccggcagca ggctgctgga gctttttgcc atgtgctgat acagctggcg atagtcgtgc
    31861 gccatggtat ccagtaattc cgcgctgcgg gcaaagtggc taaccagctc ttcgcgatac
    31921 tcgtccagtt cagctttatt cttttccagt tcgtactgca acgcctgttg ctggcgtagt
    31981 ttacgattac caaaacgcat ggccacagca ccaataatga tgccgacgac taacccaatt
    32041 agcgcatatt cccaggtcat gaacatctcc cgttgtcttt tgtttccgta gggtgttggc
    32101 ttcaggctcc atgcctgcgg ctgattatgc cactataacc gttaattcca cagaagtgga
    32161 atcccgactg catatcgcgt agtgtagaac ggcctttttt tcgtcaaccg tgaacaacgg
    32221 cgcaccgatt attcaaggaa taacaataag atcatgcaaa gcgttacccc aacatcgcaa
    32281 tacctgaagg cgctcaatga aggcagccat caacccgacg acgttcaaaa agaggccgtc
    32341 agccgcctgg aaattattta tcaagaactc atcaatagca ggccaccagc ccccaggacg
    32401 agtgggctaa tggcgcgggt cggtaagctg tggggtaaac gcgaagacac aaagcatacg
    32461 ccagtgcgtg gcttatatat gtggggcggt gtaggacgcg ggaaaacctg gctgatggac
    32521 cttttctatc aaagcctgcc gggagagcgg aaacagcgcc tgcactttca ccgttttatg
    32581 ctgcgggtgc acgaagagct aactgcctta caggggcaga ccgatccgct ggaaattatt
    32641 gccgatcgct ttaaagccga aactgacgtg ctctgttttg acgaattttt tgtttctgat
    32701 attaccgacg ccatgctact tggcggtctg atgaaagccc tgttcacccg cggtattacc
    32761 ctggtagcga cgtcaaatat tccgccggac gaactttatc gaaatggcct gcaacgtgcg
    32821 cgttttctgc ctgcaatcga tgccattaaa cagcattgtg atgtaatgaa cgtggacgct
    32881 ggtgttgatt atcgactgcg tacactcact caggcgcatc tgtggctttc gccactcaac
    32941 gatgaaaccc gggcgcagat ggataaacta tggttggcgc tggcgggggt gaaacgagaa
    33001 aattcaccga cgttagaaat caaccatcgg ccattggcga caatgggcgt cgagaaccag
    33061 acgctggcgg tttcttttac tacgctgtgc gtcgacgccc gcagtcagca tgactatatt
    33121 gcgctctcac gcctgtttca cacagtaatg ttgtttgatg tgccagtcat gacgcggttg
    33181 atggagagcg aagcgcggcg ctttattgcg ctggtggatg agttttacga gcgccatgtc
    33241 aaattagtgg tgagtgcaga agtgccgctg tatgaaattt atcagggtga gcggctgaag
    33301 tttgagttcc agcgttgcct gtcacgtctg caagagatgc aaagcgaaga gtatctgaag
    33361 cgcgagcatt tggcgggtta aaacctgcca caaatcacaa aaaggggtcg atctttgacc
    33421 ccgacttctc tataatcctg cgaccccacg ttacaagaaa gtttttttcc caaaactttt
    33481 tgtgtgctgg cataggctat tcgaaggggt aggtttgccg gactttgtcg tgtgaacctc
    33541 aacaattgaa gacgtttggg tgttcaccaa cgtgtaacta tttattgggt aagcttttaa
    33601 tgaaaacttt tacagctaaa ccagaaaccg taaaacgcga ctggtatgtt gttgacgcga
    33661 ccggtaaaac tctgggccgt ctggctactg aactggctcg tcgcctgcgc ggtaagcaca
    33721 aagcggaata tactccgcac gtagataccg gtgattacat catcgttctg aacgctgaca
    33781 aagttgctgt aaccggcaac aagcgtactg acaaagtgta ctatcaccac accggccaca
    33841 tcggtggtat caaacaagcg acctttgaag agatgattgc tcgccgtcct gagcgtgtga
    33901 ttgaaatcgc ggttaaaggc atgttgccaa aaggcccgct gggtcgtgct atgttccgta
    33961 aactgaaagt ttacgcgggt aacgagcaca accacgcggc acagcaaccg caagttcttg
    34021 acatctaatc gggattatag gcaatggctg aaaatcaata ctacggcact ggtcgccgca
    34081 aaagttccgc agctcgcgtt ttcatcaaac cgggcaacgg taaaatcgta atcaaccaac
    34141 gttctctgga acagtacttc ggtcgtgaaa ctgcccgcat ggtagttcgt cagccgctgg
    34201 aactggtcga catggttgag aaactggacc tgtacatcac cgttaaaggt ggtggtatct
    34261 ctggtcaggc tggtgcgatc cgtcacggta tcacccgcgc tctgatggaa tacgacgagt
    34321 ccctgcgttc tgaactgcgt aaagctggct tcgttactcg tgacgctcgt caggttgaac
    34381 gtaagaaagt cggtctgcgt aaagcacgtc gtcgtccgca gttctccaaa cgttaattgg
    34441 cttctgctac ggcagaaaac aatttgcgaa aaaacccgct tcggcgggtt tttttatggc
    34501 taaattctga atcagcgtaa aaaatgggaa gttgcttttt gctgccgtct gaaagacagg
    34561 taaaacaaaa accacatcgc caataagggt ctaagtcaac tatttcagac taaagcgcat
    34621 ctctttttcc ccatttccgg catccactca ccacaaaggt cgcaaaatct ggtaaactat
    34681 catccaattt tctgcccaaa tgtcgggtat tgctcatttt ttgtttgatt ttcgaacaaa
    34741 gagagtcgtt ccttattggg taacacaact tctgactggc cacctggtgg ctggtagcag
    34801 taaaaattct gactatacct ggaggttttc atggctgtcg ctgccaacaa acgttcggta
    34861 atgacgctgt tttccggtcc tactgacatc tatagccatc aggtccgcat tgtgctggct
    34921 gagaaaggtg taagtttcga gatcgaacac gtggaaaagg acaatccgcc tcaggatctg
    34981 attgacctca acccgaatca gagcgttccg accctggtgg atcgtgagct gaccctgtgg
    35041 gaatctcgca tcattatgga atatctggat gagcgtttcc cgcatccgcc actgatgcct
    35101 gtttacccgg tagctcgcgg tgaaagccgt ctgtacatgc atcgcatcga aaaagactgg
    35161 tacacgctga tgaacactat catcaacggt tcagcttctg aagcagatgc cgcacgtaag
    35221 caactgcgcg aagaactgct ggcgattgcg ccggtcttcg gtcagaagcc gtacttcctg
    35281 agcgatgagt tcagcctggt cgattgctac cttgctccgc tgctgtggcg tctgccgcaa
    35341 ctgggcatcg agttcagcgg cccgggtgcg aaagagctga aaggctatat gacccgcgtc
    35401 tttgagcgtg actctttcct tgcttcttta actgaagcag aacgtgaaat gcgtctgggc
    35461 cggagttaat ctgtatggat ttgtcacagc taacaccacg tcgtccctat ctgctgcgtg
    35521 cattctatga gtggttgctg gataaccagc tcacgccgca cctggtggtg gatgtgacgc
    35581 tccctggcgt gcaggttcct atggaatatg cgcgtgacgg gcaaatcgta ctcaacattg
    35641 cgccgcgtgc tgtcggcaat ctggaactgg cgaatgatga ggtgcgcttt aacgcgcgct
    35701 ttggtggcat tccgcgtcag gtttctgtgc cgctggctgc cgtgctggct atctacgccc
    35761 gtgaaaatgg cgcaggcacg atgtttgagc ctgaagctgc ctacgatgaa gataccagca
    35821 tcatgaatga tgaagaggca tcggcagaca acgaaaccgt tatgtcggtt attgatggcg
    35881 acaagccaga tcacgatgat gacactcatc ctgacgatga acctccgcag ccaccacgcg
    35941 gtggtcgacc ggcattacgc gttgtgaagt aatacaaaac aggcccaggc ggcctgtttt
    36001 gtctttttaa tgacttaata aaatagcgtg attgtggcaa tcatattcac cacgaaaccg
    36061 actgccatgg ggatcgctgt ccgcttcacc accgcaaaag gcgaaacgcc tgcaattcct
    36121 gacgtaacga ccaccaccgc agtaatcggc gaaaccgcgc gcgctagcgt cgtggcaaaa
    36181 tgcatcggca taatcattac aaccgctggt acatgtagtc cggctgcgat attcggaata
    36241 agactggcaa atgacataaa cggcgcattg ccagagccca tcacaatggc acaaatggca
    36301 atgactagcg ccataataat catcacgcca ataccgccca gaccagaatg ctccgcaccc
    36361 ctgataaccg catcaacagt gccaatcgtc gttaagcctt tcgcaaatat ttcacccgcg
    36421 accaccagcg ttaccacgtt gacaaactgc gtacccatgc cgtcaaaaaa cgcctgcaca
    36481 tcgtccattg tttcgcgcaa gttatgcttg cggaagaact caacaaacat cgtcaccgtc
    36541 aaacttagta acatcaccac caccagatgc agttctgact gcatcagccc gacgtgggcg
    36601 aggaacagcg agccgagcat caggattaac ggcatcacag gtaaaatggc gtaatagagc
    36661 ggcgggacat tatcgagagt tttttgctct gcctgttcgt gattgatatt tttatctttt
    36721 ttgtcaaaag cgcgttgcac gaaaaagtgg gagatcgcca ccgagataat gacgcaagag
    36781 gcgaccggaa gctggtagtg gaagaagtat gtggcaattt tcattcccgc gacctgggct
    36841 gcaaaaatgg agttcgtttc cagaatcccc cactcaatgg acatcgtggt tgcgataacc
    36901 gccaccgcag agaggcgact tactcccaga ctcaccagcg tcggaaataa ggtgaccatc
    36961 agcaacatac ccagaccgga ggcgctggta ataaactgcg ccatgatttg gccgatgacg
    37021 taagttgccg acagaataat atacggcgag cgaatgagtt ttaacgggcg gcttagcaag
    37081 ctcaccatcg cgcaactggc tcctatgcgc tccatgtagc gggcataacc gcccaccgcc
    37141 ataatcgaca gccccaatcc ggcaatgcgg ttggacagca tgcgcaggat ttcattataa
    37201 atgtcgacga tcaagtagcc gctgcttttg gcgggatcga gtacaccacc gaaccctaac
    37261 cagactccgc acatcatcag gaagacacct gcggcagcca gcaccacctg aggtttgtag
    37321 tttttcagga tcaaatagcc catcgtaatt aatacgatga cagatataat tatgccgaac
    37381 att
//
LOCUS       DAJTRN010000031        34989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRN010000031 DAJTRN010000000
VERSION     DAJTRN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..805)
                     /locus_tag="OO328_001613"
     CDS             complement(<1..805)
                     /locus_tag="OO328_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaH family type III secretion system E3
                     ubiquitin--protein ligase"
                     /protein_id="HCR5930216.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="OO328_001614"
                     /pseudo
     CDS             897..983
                     /locus_tag="OO328_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1388..1864)
                     /locus_tag="OO328_001615"
     CDS             complement(1388..1864)
                     /locus_tag="OO328_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5930217.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="OO328_001616"
     CDS             complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="OO328_001616"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5930218.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3299..4339
                     /gene="bioB"
                     /locus_tag="OO328_001617"
     CDS             3299..4339
                     /gene="bioB"
                     /locus_tag="OO328_001617"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5930219.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4336..5490
                     /gene="bioF"
                     /locus_tag="OO328_001618"
     CDS             4336..5490
                     /gene="bioF"
                     /locus_tag="OO328_001618"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5930220.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5477..6232
                     /gene="bioC"
                     /locus_tag="OO328_001619"
     CDS             5477..6232
                     /gene="bioC"
                     /locus_tag="OO328_001619"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5930221.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6225..6902
                     /gene="bioD"
                     /locus_tag="OO328_001620"
     CDS             6225..6902
                     /gene="bioD"
                     /locus_tag="OO328_001620"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5930222.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="OO328_001621"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="OO328_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5930223.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="OO328_001622"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="OO328_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5930224.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="OO328_001623"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="OO328_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5930225.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="OO328_001624"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="OO328_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5930226.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="OO328_001625"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="OO328_001625"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5930227.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="OO328_001626"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="OO328_001626"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5930228.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="OO328_001627"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="OO328_001627"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5930229.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13839..14543
                     /locus_tag="OO328_001628"
     CDS             13839..14543
                     /locus_tag="OO328_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5930230.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14748..15461
                     /locus_tag="OO328_001629"
     CDS             14748..15461
                     /locus_tag="OO328_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5930231.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15497..16453)
                     /locus_tag="OO328_001630"
     CDS             complement(15497..16453)
                     /locus_tag="OO328_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5930232.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="OO328_001631"
     CDS             complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="OO328_001631"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5930233.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17691..18452)
                     /locus_tag="OO328_001632"
     CDS             complement(17691..18452)
                     /locus_tag="OO328_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5930234.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18585..18995
                     /locus_tag="OO328_001633"
     CDS             18585..18995
                     /locus_tag="OO328_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5930235.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18957..20063)
                     /locus_tag="OO328_001634"
     CDS             complement(18957..20063)
                     /locus_tag="OO328_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5930236.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20074..21207)
                     /locus_tag="OO328_001635"
     CDS             complement(20074..21207)
                     /locus_tag="OO328_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5930237.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21200..22936)
                     /locus_tag="OO328_001636"
     CDS             complement(21200..22936)
                     /locus_tag="OO328_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5930238.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="OO328_001637"
     CDS             complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="OO328_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5930239.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="OO328_001638"
     CDS             complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="OO328_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5930240.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24827..26014
                     /gene="rhlE"
                     /locus_tag="OO328_001639"
                     /pseudo
     CDS             24827..26014
                     /gene="rhlE"
                     /locus_tag="OO328_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="OO328_001640"
     CDS             complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="OO328_001640"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5930241.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26823..28973
                     /gene="dinG"
                     /locus_tag="OO328_001641"
     CDS             26823..28973
                     /gene="dinG"
                     /locus_tag="OO328_001641"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5930242.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29001..29963
                     /gene="ybiB"
                     /locus_tag="OO328_001642"
     CDS             29001..29963
                     /gene="ybiB"
                     /locus_tag="OO328_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5930243.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30104..31189
                     /gene="hcxB"
                     /locus_tag="OO328_001643"
     CDS             30104..31189
                     /gene="hcxB"
                     /locus_tag="OO328_001643"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5930244.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31418..31678)
                     /locus_tag="OO328_001644"
     CDS             complement(31418..31678)
                     /locus_tag="OO328_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5930245.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31943..32209)
                     /gene="ybiI"
                     /locus_tag="OO328_001645"
     CDS             complement(31943..32209)
                     /gene="ybiI"
                     /locus_tag="OO328_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5930246.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32283..32960)
                     /gene="ybiX"
                     /locus_tag="OO328_001646"
     CDS             complement(32283..32960)
                     /gene="ybiX"
                     /locus_tag="OO328_001646"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5930247.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33002..34989)
                     /locus_tag="OO328_001647"
                     /pseudo
     CDS             complement(33002..34989)
                     /locus_tag="OO328_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8291 a         8972 c         9452 g         8274 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg ggaaggtaaa
     1321 aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc gccatctggc
     1381 agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg aaatgaacgt
     1441 taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct acatccagcg
     1501 cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc
     1561 cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag
     1621 agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact acccagtgcc
     1681 accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc
     1741 ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc
     1801 cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta ctgatgagtt
     1861 tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac gctgacttcc
     1921 cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc
     1981 tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc
     2041 cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt caccggacga
     2101 gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat ttcggcatca
     2161 cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg ctgctgccat
     2221 tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag cggattgccc
     2281 ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt ttctgcaacc
     2341 tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgagg
     2401 cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc ggtacgacca
     2461 aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg
     2521 attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg gacaatcggc
     2581 tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc aaaaccaacc
     2641 atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc aaacaagttt
     2701 tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca caccgacatc
     2761 gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg
     2821 cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc tacggaaccg
     2881 gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag tttgcggcat
     2941 agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat ggcatcaatt
     3001 tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc cgcccaccag
     3061 gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca accttcggcg
     3121 ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg ccagatatgg
     3181 cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga
     3241 tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga gaagccccat
     3301 ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa aaccgttgct
     3361 ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc gtcaggtgca
     3421 ggtcagcacg ttgctgtcga ttaagactgg agcttgtccg gaagattgca aatactgccc
     3481 gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag ttgaacaggt
     3541 gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta tgggcgcggc
     3601 atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc agggggtaaa
     3661 agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc aggcgcagcg
     3721 cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc cggagtttta
     3781 cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg aaaaagtgcg
     3841 cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa cggtaaaaga
     3901 tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa gcgtaccaat
     3961 caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg ttgatgcctt
     4021 tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt acgtgcgcct
     4081 ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta tggcaggcgc
     4141 aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag aagataaaga
     4201 cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc tggcagggga
     4261 taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg acgaatatta
     4321 caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt
     4381 gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg ctggctggtg
     4441 gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt aagccatcat
     4501 ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg tagcggcggc
     4561 tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga actggccgag
     4621 tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa tcaggcagtc
     4681 attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag ccatgcctca
     4741 ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca taacgatgtc
     4801 gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt ggtgacagaa
     4861 ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca ggtaacgcaa
     4921 cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat cggggagcag
     4981 gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt gacttttggc
     5041 aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc ggattatctg
     5101 ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca ggcgcaggca
     5161 ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg cgaaaaactg
     5221 gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac gctggctgat
     5281 tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt acaactggca
     5341 gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac cgtacccgct
     5401 ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga tatcgaccgt
     5461 ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg catttggtcg
     5521 ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg acgccttact
     5581 ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg gacctggctg
     5641 gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc tctcgccgcc
     5701 aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag gagatatcga
     5761 atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg cggtgcagtg
     5821 gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt
     5881 ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg cgcggcaggc
     5941 ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg aagagtcgct
     6001 gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg atgatgcgct
     6061 cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag ggcgcgaccc
     6121 gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc aacaacaggg
     6181 gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt aaacgttatt
     6241 ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca cttttacaag
     6301 ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct ggcagcgaaa
     6361 agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac agcagcctgc
     6421 agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg ccgcacatca
     6481 tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga ttacgcgcgc
     6541 ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt acgccgcttt
     6601 ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg atactggtag
     6661 ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca atacaacacg
     6721 ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga aaacgtcacg
     6781 ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga gagatcccct
     6841 ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tgtccgccac
     7861 caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg aatatctcga
     8881 agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg agcgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca acttactgcg
     9001 tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agttgcgtga
     9481 gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa cggaaaacag
     9541 tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa tttgcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcaacagg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgtt
    10381 cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc atttgaggcc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgatgaa gccatagtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt gattacgggt
    12241 ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt gttcgaccgt
    12301 gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt attcaggcgg
    12721 caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggcgga
    13321 gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc ataacctggg
    13381 cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatct
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg cagatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc ttcgcttggc
    15541 tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa tacaggacgc
    15601 gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca gccagtagtg
    15661 cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg acgccagcaa
    15721 tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga ccaagtaaca
    15781 gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc tgagcgaggg
    15841 cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg cggtgcttcg
    15901 cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg ccgagaatac
    15961 gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct gcggtaaaga
    16021 taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc gaggaaatcc
    16081 gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc ataccaatgc
    16141 cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac accagcatca
    16201 cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca tagcagccgt
    16261 aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg cgattgtagt
    16321 cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc accagcaaca
    16381 caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc cagcgcgggt
    16441 gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat cctgcgtttc
    16501 cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg ccggaagcca
    16561 gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt tggtcaggtt
    16621 ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg cggcaataat
    16681 gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga tgacatttgc
    16741 ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg tcgcccagtg
    16801 atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa aaacctgaac
    16861 gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa tcggcatatc
    16921 cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt tacgcaaggc
    16981 gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt cccgctgcgc
    17041 ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt cgttatcgcg
    17101 ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt tgtgatgacg
    17161 tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa actggagaat
    17221 atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct ctggaccgta
    17281 gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac gcgcgtcgat
    17341 caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa aaaggcgagg
    17401 gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga caaactcatc
    17461 gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc cgcgttgcgc
    17521 tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc agataaacgt
    17581 ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg gataatattg
    17641 ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt tcataaatga
    17701 atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag cggcaacgcg
    17761 gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag tagaggaaat
    17821 tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc atccagtccg
    17881 gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc acccgccacc
    17941 aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc aagctgcgcc
    18001 tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc ttttccggtc
    18061 atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc gaccgaaaca
    18121 tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc gtgatgccct
    18181 tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc ggcgagaaac
    18241 tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc gtgcgcgccc
    18301 atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc gcgaagttcc
    18361 ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat ggtgagcacc
    18421 ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc tcttatctca
    18481 ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt ccgtaaagtt
    18541 cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag tggcaacaac
    18601 gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta ctggtagtgg
    18661 cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg ggattatgcg
    18721 cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc gagcaacgct
    18781 ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt ggcgcagcgc
    18841 tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg gcaattgcag
    18901 ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat aagaagttac
    18961 atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt tatcaccagt
    19021 agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata aatctgcttg
    19081 gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag ccacactggc
    19141 atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat aaaaacaaac
    19201 acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag accgaatccc
    19261 accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga tccggcgaag
    19321 gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa ggtggcgaca
    19381 attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg cgaaaccagt
    19441 agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt gacgatcatt
    19501 acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt gtagtcgaga
    19561 ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg ttttccttcc
    19621 agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc aatttgcgca
    19681 ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa ggtatccagt
    19741 ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt gtcgatggtc
    19801 gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct ggcgcgggca
    19861 aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat ggcgatggtg
    19921 gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat tagcacgggt
    19981 aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc tttgcggatt
    20041 aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt tggttttcag
    20101 ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt ttaccaccag
    20161 caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga aataacgagc
    20221 gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct gaaaaataaa
    20281 gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag cggcattgaa
    20341 ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga gtaaaaacag
    20401 gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga gaataaacac
    20461 tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag ggatcagctt
    20521 acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca tggtgccgcg
    20581 ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg tcatgataat
    20641 ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa accagtagcg
    20701 ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc gctgcatttg
    20761 ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat tcggctcact
    20821 gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct gttcggcgaa
    20881 atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg cgatcagttc
    20941 ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa
    21001 atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct tgctggagtc
    21061 gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta ccgcaatcag
    21121 ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg cccgcacgcg
    21181 atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg tggatcaact
    21241 gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac tgtgctttca
    21301 aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg atgcggtcgc
    21361 aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct ttctctacca
    21421 tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg gaagtcggtt
    21481 cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt
    21541 taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg ccaaatgcct
    21601 cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac acaccagaga
    21661 aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt tgcgccatat
    21721 agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc agcaccagcg
    21781 cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc gatttacccg
    21841 cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag ttgacgtgat
    21901 cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc accgtctcgc
    21961 cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag gttccggcac
    22021 cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg atgttgattt
    22081 ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg aggatcagac
    22141 gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag gcgcgttgca
    22201 acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg gccatggttt
    22261 gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc agtaacacgt
    22321 cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac atcccttcgc
    22381 ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca acgccgacgc
    22441 cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc aggccgagtt
    22501 tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg
    22561 taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat
    22621 ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca aatttctgcg
    22681 gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca aagccaatca
    22741 ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc agtgtggttt
    22801 tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg gtacaatcga
    22861 gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga
    22921 taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg cattccctgg
    22981 cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag gtcggtacgc
    23041 agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac gaaaccaatc
    23101 tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt gcgccccggc
    23161 tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg acgcgttagt
    23221 gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac cgcgcgcgtt
    23281 aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag ctccgcctgc
    23341 gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc ctgttcacgg
    23401 ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc ctgtgcctgg
    23461 tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct tttccacagt
    23521 ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc
    23581 gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag atcatactgc
    23641 gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata cggcttgtga
    23701 tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac cgccagcgat
    23761 tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac gttgccatac
    23821 agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc aaccacggcg
    23881 gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac aaactccttt
    23941 gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg atcagttcgg
    24001 tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct ttgccaagac
    24061 ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg gtgtcattag
    24121 cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc ggactaatca
    24181 cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg gagataaact
    24241 tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg caggcgcgaa
    24301 ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag cgttcggctt
    24361 cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg gcgcaggcga
    24421 ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc tgcccggcct
    24481 gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc gccagtgcgg
    24541 cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga ttattcatcg
    24601 cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg gagttgtcca
    24661 gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct gcgtcacaaa
    24721 aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct tttcagtatc
    24781 tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt ctttcgattc
    24841 tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc gtgaacccac
    24901 ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga tggctagcgc
    24961 ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac acctgatcac
    25021 tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg
    25081 tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc tgaacattcg
    25141 ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt tgcgcggcgg
    25201 cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc aaaatgcagt
    25261 gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg
    25321 ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc agaacctgct
    25381 gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt tgcacaaccc
    25441 gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc agcacgttca
    25501 ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta aagggaactg
    25561 gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg ctgagcagct
    25621 caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag gtgcgcgtac
    25681 ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga ccgatatcgc
    25741 tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac tgccaaacgt
    25801 accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc
    25861 gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa aactgctgaa
    25921 aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa tcaaagccga
    25981 accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca aacgcaaaac
    26041 tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg aatttgcatg
    26101 cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca agcacttgtc
    26161 agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc caccgagacg
    26221 ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca gtcgattctt
    26281 gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct caaccagttt
    26341 tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga atttagcgcg
    26401 aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa gtggcttgga
    26461 acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga tctcttcgcg
    26521 gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg gccaggtatt
    26581 cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt catcgctggt
    26641 gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg
    26701 cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca gaaaaaccat
    26761 tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc
    26821 tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg
    26881 aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca
    26941 aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc ggcgttggga
    27001 aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg
    27061 tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat ttaccgctgc
    27121 tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg cgctacgttt
    27181 gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat ctgctggcgt
    27241 ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt gcgaagctga
    27301 agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat atcgctatag
    27361 atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac cgtaactgtt
    27421 actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa gcggaagtgg
    27481 tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ctgcctgacc
    27541 cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg gcccgggatg
    27601 cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg gacttgttca
    27661 cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca ccgctggcga
    27721 tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca
    27781 acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg
    27841 aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc
    27901 gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc cacgatattg
    27961 tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt gaggtgcaaa
    28021 gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg
    28081 cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg ggaatacgtg
    28141 tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacctccg
    28201 caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt ctgaaagaga
    28261 aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa
    28321 ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa cacattgccg
    28381 aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt atgttggtac
    28441 tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat ttacgcctga
    28501 tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac cgcaaacgcg
    28561 ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa gggcttgatt
    28621 tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg cccatcgaca
    28681 gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg
    28741 tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga ctgattcgaa
    28801 gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca aaaaattatg
    28861 gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag
    28921 gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg
    28981 agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga
    29041 aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct catatgctca
    29101 atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt atcaaagggg
    29161 aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga
    29221 cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac
    29281 aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt cctgtggtgg
    29341 ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc gaactgatgg
    29401 gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg
    29461 tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc
    29521 ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag
    29581 gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc gtcgcgaagt
    29641 tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc gaagtgtatg
    29701 ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtgctgt
    29761 atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca
    29821 cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa
    29881 tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc gatggcctgg
    29941 cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt catccggctt
    30001 ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc ccggcgtgca
    30061 gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa gtggtcatcg
    30121 ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga tgggtagcga
    30181 ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg cagggcatga
    30241 ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca
    30301 aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga
    30361 tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg agaaagcgca
    30421 tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc gtatcggtta
    30481 ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta gcgtggtcgg
    30541 tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atcccttctg
    30601 tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca ccagcgccat
    30661 tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct
    30721 gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt cgccgttggg
    30781 gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct
    30841 tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga
    30901 tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg gcgcgccgga
    30961 ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc atgatgacga
    31021 taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca
    31081 ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc ggcagattgg
    31141 tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg
    31201 tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg
    31261 tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg cctgatccgt
    31321 ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt
    31381 gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac
    31441 cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca ccggctgccg
    31501 ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca tcggcagaaa
    31561 ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc gccgcgaaca
    31621 cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa
    31681 ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc
    31741 acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa ataaattgat
    31801 tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg ttttatgccg
    31861 cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa attatactcg
    31921 ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct ctgcgattat
    31981 atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata cataagcgca
    32041 cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct tcacattcat
    32101 acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct tcaattgtac
    32161 tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc
    32221 catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg gcagttcaag
    32281 cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag cgacaggatc
    32341 tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag ttcaaacagc
    32401 atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga tgccactcgt
    32461 acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa caccagatca
    32521 cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag ttcgccgccg
    32581 tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg catccagccg
    32641 ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc attgttctga
    32701 tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca
    32761 tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg
    32821 tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc
    32881 acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg
    32941 ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc ccacatctga
    33001 atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg tgataacggt
    33061 agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa acgtttagct
    33121 ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc cagtaacctt
    33181 ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg ccgatatagc
    33241 gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat aaggtgaagg
    33301 cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct ttgccgtttt
    33361 tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga gtgatattcc
    33421 ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag taagtcccgt
    33481 catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca
    33541 ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct ttttgcggtt
    33601 taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg aagttgttgc
    33661 cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt tccgtcaggt
    33721 ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg tcgacggtgg
    33781 tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc
    33841 cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc ccgccgttca
    33901 gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct
    33961 gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat
    34021 gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc tgcgtttcac
    34081 gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag aacgtcgagg
    34141 tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc gccgtgcgtg
    34201 actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc ccgcccataa
    34261 tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg
    34321 tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt
    34381 aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga ttcagggccg
    34441 ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg
    34501 gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct gtaccaaggc
    34561 caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg cgtccggcat
    34621 cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca atgacctgat
    34681 tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag gcgtcaatgc
    34741 cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc
    34801 cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg aaggtgtcgc
    34861 gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag gtatcggcac
    34921 cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac
    34981 ccacgccgg
//
LOCUS       DAJTRN010000032        34790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRN010000032 DAJTRN010000000
VERSION     DAJTRN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..2098
                     /locus_tag="OO328_001648"
     CDS             <1..2098
                     /locus_tag="OO328_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5930248.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO328_001649"
     CDS             2095..3183
                     /locus_tag="OO328_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5930249.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO328_001650"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO328_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5930250.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO328_001651"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO328_001651"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5930251.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO328_001652"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO328_001652"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5930252.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO328_001653"
     CDS             8051..8602
                     /locus_tag="OO328_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5930253.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO328_001654"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO328_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5930254.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO328_001655"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO328_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5930255.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO328_001656"
     CDS             complement(11683..12681)
                     /locus_tag="OO328_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5930256.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO328_001657"
     CDS             complement(12760..13452)
                     /locus_tag="OO328_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5930257.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO328_001658"
     CDS             complement(13529..14032)
                     /locus_tag="OO328_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5930258.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO328_001659"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO328_001659"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5930259.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO328_001660"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO328_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5930260.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO328_001661"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO328_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5930261.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO328_001662"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO328_001662"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5930262.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO328_001663"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO328_001663"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5930263.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO328_001664"
     CDS             complement(20922..21341)
                     /locus_tag="OO328_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930264.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO328_001665"
     CDS             complement(21319..21672)
                     /locus_tag="OO328_001665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930265.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO328_001666"
     CDS             complement(21673..21993)
                     /locus_tag="OO328_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930266.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO328_001667"
     CDS             complement(22127..23560)
                     /locus_tag="OO328_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5930267.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO328_001668"
     CDS             complement(23623..24072)
                     /locus_tag="OO328_001668"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5930268.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO328_001669"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO328_001669"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5930269.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO328_001670"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO328_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5930270.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO328_001671"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO328_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO328_001672"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO328_001672"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5930271.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO328_001673"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO328_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO328_001674"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO328_001674"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5930272.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO328_001675"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO328_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO328_001676"
     tRNA            complement(34082..34157)
                     /locus_tag="OO328_001676"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="OO328_001677"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="OO328_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8486 a         8945 c         9141 g         8218 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcacaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa
//
LOCUS       DAJTRN010000033        34760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRN010000033 DAJTRN010000000
VERSION     DAJTRN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="OO328_001678"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="OO328_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5930273.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="OO328_001679"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="OO328_001679"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5930274.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="OO328_001680"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="OO328_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5930275.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO328_001681"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO328_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5930276.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="OO328_001682"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="OO328_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO328_001683"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO328_001683"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5930277.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO328_001684"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO328_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5930278.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="OO328_001685"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="OO328_001685"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5930279.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="OO328_001686"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="OO328_001686"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5930280.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="OO328_001687"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="OO328_001687"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5930281.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="OO328_001688"
     CDS             12897..13847
                     /locus_tag="OO328_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5930282.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="OO328_001689"
     CDS             complement(13888..14358)
                     /locus_tag="OO328_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5930283.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="OO328_001690"
     CDS             complement(14413..15615)
                     /locus_tag="OO328_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5930284.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="OO328_001691"
     CDS             complement(15702..16244)
                     /locus_tag="OO328_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930285.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="OO328_001692"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="OO328_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5930286.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="OO328_001693"
     CDS             complement(16987..17589)
                     /locus_tag="OO328_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5930287.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="OO328_001694"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="OO328_001694"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5930288.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="OO328_001695"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="OO328_001695"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5930289.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="OO328_001696"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="OO328_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /locus_tag="OO328_001697"
     CDS             21986..22876
                     /locus_tag="OO328_001697"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5930290.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="OO328_001698"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="OO328_001698"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5930291.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="OO328_001699"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="OO328_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5930292.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="OO328_001700"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="OO328_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5930293.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="OO328_001701"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="OO328_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5930294.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="OO328_001702"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="OO328_001702"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5930295.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="OO328_001703"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="OO328_001703"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5930296.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="OO328_001704"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="OO328_001704"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5930297.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="OO328_001705"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="OO328_001705"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5930298.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="OO328_001706"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="OO328_001706"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5930299.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="OO328_001707"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="OO328_001707"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5930300.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="OO328_001708"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="OO328_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5930301.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="OO328_001709"
     CDS             complement(33051..33512)
                     /locus_tag="OO328_001709"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5930302.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="OO328_001710"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="OO328_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5930303.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         8490 a         9322 c         8613 g         8335 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat agagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttatcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg
//
LOCUS       DAJTRN010000034        34734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRN010000034 DAJTRN010000000
VERSION     DAJTRN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(18..93)
                     /locus_tag="OO328_001711"
     tRNA            complement(18..93)
                     /locus_tag="OO328_001711"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="OO328_001712"
     tRNA            complement(152..228)
                     /locus_tag="OO328_001712"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="OO328_001713"
     CDS             complement(331..1716)
                     /locus_tag="OO328_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5930304.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO328_001714"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO328_001714"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5930305.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO328_001715"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO328_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5930306.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO328_001716"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO328_001716"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5930307.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO328_001717"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO328_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5930308.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO328_001718"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO328_001718"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5930309.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO328_001719"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO328_001719"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5930310.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO328_001720"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO328_001720"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5930311.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO328_001721"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO328_001721"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5930312.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO328_001722"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO328_001722"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5930313.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO328_001723"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO328_001723"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5930314.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO328_001724"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO328_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5930315.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO328_001725"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO328_001725"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5930316.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO328_001726"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO328_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5930317.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO328_001727"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO328_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5930318.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO328_001728"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO328_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5930319.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="OO328_001729"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="OO328_001729"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5930320.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="OO328_001730"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="OO328_001730"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5930321.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO328_001731"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO328_001731"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5930322.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="OO328_001732"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="OO328_001732"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5930323.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO328_001733"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO328_001733"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5930324.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="OO328_001734"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="OO328_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5930325.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO328_001735"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO328_001735"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5930326.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO328_001736"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO328_001736"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5930327.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO328_001737"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO328_001737"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5930328.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO328_001738"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO328_001738"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5930329.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /locus_tag="OO328_001739"
     CDS             complement(29521..31167)
                     /locus_tag="OO328_001739"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5930330.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /locus_tag="OO328_001740"
     CDS             complement(31170..31220)
                     /locus_tag="OO328_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930331.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO328_001741"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO328_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5930332.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33235
                     /locus_tag="OO328_001742"
                     /pseudo
     CDS             31756..33235
                     /locus_tag="OO328_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO328_001743"
     CDS             complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO328_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5930333.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33717..34556
                     /gene="hdfR"
                     /locus_tag="OO328_001744"
     CDS             33717..34556
                     /gene="hdfR"
                     /locus_tag="OO328_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5930334.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34652..34727)
                     /locus_tag="OO328_001745"
     tRNA            complement(34652..34727)
                     /locus_tag="OO328_001745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34692..34694),aa:Trp,seq:cca)
BASE COUNT         8475 a         9498 c         8772 g         7989 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgtgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtacgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gctcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacggac gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc aatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca ctggagatcc cattaccacc
    32881 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    32941 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga
    33001 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaaga
    33061 aacgctgatc catctggggt tatcgattcg tgcctggcag cggttactga aagttgcacg
    33121 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
    33181 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
    33241 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    33301 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    33361 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    33421 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    33481 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    33541 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    33601 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    33661 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    33721 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    33781 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    33841 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    33901 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    33961 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat
    34021 gtatgcttaa tcagtggttg ggacgcttgt atcaaaatca ggatgcccat acaggcttac
    34081 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    34141 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    34201 ggtatttcac tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact
    34261 atctgcgact ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    34321 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    34381 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    34441 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    34501 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatctg
    34561 aatggctggc aaggatgccg gtagaaggat ttacttcgga gaggattatt tcagataaaa
    34621 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    34681 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagg
//
LOCUS       DAJTRN010000035        34548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRN010000035 DAJTRN010000000
VERSION     DAJTRN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(139..477)
                     /locus_tag="OO328_001746"
     CDS             complement(139..477)
                     /locus_tag="OO328_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930335.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKV"
     gene            847..1797
                     /gene="corA"
                     /locus_tag="OO328_001747"
     CDS             847..1797
                     /gene="corA"
                     /locus_tag="OO328_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5930336.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1838..2218)
                     /locus_tag="OO328_001748"
     CDS             complement(1838..2218)
                     /locus_tag="OO328_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5930337.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2232..2648)
                     /locus_tag="OO328_001749"
     CDS             complement(2232..2648)
                     /locus_tag="OO328_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930338.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2707..3597)
                     /gene="rarD"
                     /locus_tag="OO328_001750"
     CDS             complement(2707..3597)
                     /gene="rarD"
                     /locus_tag="OO328_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5930339.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3649..4116)
                     /locus_tag="OO328_001751"
     CDS             complement(3649..4116)
                     /locus_tag="OO328_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5930340.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4281..5150
                     /gene="pldA"
                     /locus_tag="OO328_001752"
     CDS             4281..5150
                     /gene="pldA"
                     /locus_tag="OO328_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5930341.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5283..7112
                     /gene="recQ"
                     /locus_tag="OO328_001753"
     CDS             5283..7112
                     /gene="recQ"
                     /locus_tag="OO328_001753"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5930342.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7176..7796
                     /gene="rhtC"
                     /locus_tag="OO328_001754"
     CDS             7176..7796
                     /gene="rhtC"
                     /locus_tag="OO328_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5930343.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7858..8478)
                     /gene="rhtB"
                     /locus_tag="OO328_001755"
     CDS             complement(7858..8478)
                     /gene="rhtB"
                     /locus_tag="OO328_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5930344.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8589..9611
                     /gene="pldB"
                     /locus_tag="OO328_001756"
     CDS             8589..9611
                     /gene="pldB"
                     /locus_tag="OO328_001756"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5930345.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9619..10419
                     /gene="yigL"
                     /locus_tag="OO328_001757"
     CDS             9619..10419
                     /gene="yigL"
                     /locus_tag="OO328_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5930346.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10495..11394
                     /gene="bioP"
                     /locus_tag="OO328_001758"
     CDS             10495..11394
                     /gene="bioP"
                     /locus_tag="OO328_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5930347.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11282..12235)
                     /gene="metR"
                     /locus_tag="OO328_001759"
     CDS             complement(11282..12235)
                     /gene="metR"
                     /locus_tag="OO328_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5930348.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12471..14732
                     /gene="metE"
                     /locus_tag="OO328_001760"
     CDS             12471..14732
                     /gene="metE"
                     /locus_tag="OO328_001760"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5930349.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14771..15586)
                     /locus_tag="OO328_001761"
     CDS             complement(14771..15586)
                     /locus_tag="OO328_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5930350.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15848..16609
                     /gene="udp"
                     /locus_tag="OO328_001762"
     CDS             15848..16609
                     /gene="udp"
                     /locus_tag="OO328_001762"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5930351.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16750..18177
                     /gene="rmuC"
                     /locus_tag="OO328_001763"
     CDS             16750..18177
                     /gene="rmuC"
                     /locus_tag="OO328_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5930352.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18272..19027
                     /gene="ubiE"
                     /locus_tag="OO328_001764"
     CDS             18272..19027
                     /gene="ubiE"
                     /locus_tag="OO328_001764"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5930353.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            19041..19646
                     /gene="ubiJ"
                     /locus_tag="OO328_001765"
     CDS             19041..19646
                     /gene="ubiJ"
                     /locus_tag="OO328_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5930354.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19643..21283
                     /gene="ubiB"
                     /locus_tag="OO328_001766"
     CDS             19643..21283
                     /gene="ubiB"
                     /locus_tag="OO328_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5930355.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21362..21631
                     /gene="tatA"
                     /locus_tag="OO328_001767"
     CDS             21362..21631
                     /gene="tatA"
                     /locus_tag="OO328_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5930356.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21635..22150
                     /gene="tatB"
                     /locus_tag="OO328_001768"
     CDS             21635..22150
                     /gene="tatB"
                     /locus_tag="OO328_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5930357.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22153..22929
                     /gene="tatC"
                     /locus_tag="OO328_001769"
     CDS             22153..22929
                     /gene="tatC"
                     /locus_tag="OO328_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5930358.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22971..23753
                     /gene="tatD"
                     /locus_tag="OO328_001770"
     CDS             22971..23753
                     /gene="tatD"
                     /locus_tag="OO328_001770"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5930359.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23750..24238)
                     /gene="rfaH"
                     /locus_tag="OO328_001771"
     CDS             complement(23750..24238)
                     /gene="rfaH"
                     /locus_tag="OO328_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5930360.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24405..25898
                     /gene="ubiD"
                     /locus_tag="OO328_001772"
     CDS             24405..25898
                     /gene="ubiD"
                     /locus_tag="OO328_001772"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5930361.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25944..26645
                     /gene="fre"
                     /locus_tag="OO328_001773"
     CDS             25944..26645
                     /gene="fre"
                     /locus_tag="OO328_001773"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5930362.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26837..28000)
                     /gene="fadA"
                     /locus_tag="OO328_001774"
     CDS             complement(26837..28000)
                     /gene="fadA"
                     /locus_tag="OO328_001774"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5930363.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(28010..30199)
                     /gene="fadB"
                     /locus_tag="OO328_001775"
     CDS             complement(28010..30199)
                     /gene="fadB"
                     /locus_tag="OO328_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5930364.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30389..31720
                     /gene="pepQ"
                     /locus_tag="OO328_001776"
     CDS             30389..31720
                     /gene="pepQ"
                     /locus_tag="OO328_001776"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5930365.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31720..32334
                     /locus_tag="OO328_001777"
     CDS             31720..32334
                     /locus_tag="OO328_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5930366.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32373..33824
                     /gene="trkH"
                     /locus_tag="OO328_001778"
     CDS             32373..33824
                     /gene="trkH"
                     /locus_tag="OO328_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5930367.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33836..34381
                     /gene="hemG"
                     /locus_tag="OO328_001779"
     CDS             33836..34381
                     /gene="hemG"
                     /locus_tag="OO328_001779"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5930368.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8142 a         8722 c         9332 g         8352 t
ORIGIN      
        1 gcgatcttgc aagtatggga caacttgtga aattaccgac gagaagggga agggccttcc
       61 cgagagtggt aaaggagagg ccctggaaat accccacagc cccgaaaaag agccagtcag
      121 ttgcttaact gactggcatt acaccttctg ctgaccgttt tcgatgtaca aaccgagcgg
      181 cgcatagctt tcatcataga tgccgccgtt catcgccatc tgcacctgac cctgactatt
      241 aatatccgcc agcagagcat gtaacgttcc ccacgcttcg ccattggctt tttgccagta
      301 cattttcacc cgctctgttt gaggattaac ggtatacgcc tgtacggtca gcgtcggatc
      361 tgagagtgcg caatcatcag cggcaacagc aaacaaggga agtaaggtga gggtgagaaa
      421 aatccgtttg agattcaagg tgatcattcc tttaccaatg agtagccgat gcgccattat
      481 aggtcctaga tgtgggattt ttttatcctg ttagcgacct tgacgagtac caaaaagcgc
      541 gaagttcaac tattgttctg tggtgttctg ttgcgtgttg acggcaaaat tttgctggcg
      601 taacatgcgc gcacgatcac tctaagagga cattcgcctt ggacacaccc agtagatact
      661 ggctcactat cctgtcatcc aggatcaact cctaaggcta tccctttttg ctgatagcct
      721 tagcggttgt cagcgacctc aatttttccc gtcgcgctga gtcaggctgt ttaatggtct
      781 gaaacccaat ttgtttctgt gtgcccaccg aactgtccga tattttaagc attgggagtc
      841 ccggtcatgc tgagcgcatt tcaactggaa aataaccgac tgacccggct ggaagtcgaa
      901 gagtcacaac cccttgtaaa tgcagtatgg attgatcttg tcgaaccgga cgacgacgag
      961 cgactgcgcg tacaatctga acttggccag agcctggcaa cccgcccgga actggaagac
     1021 atcgaagcat cggcacgttt ctttgaagac gacgacggcc tgcatattca ctccttcttc
     1081 ttctttgaag atgcggaaga tcacgccggt aactccactg tggcatttac catccgtgat
     1141 ggtcgtctgt ttactctgcg tgagcgtgaa ctgcccgctt ttcgtctgta tcgtatgcgt
     1201 gcccgtagcc agtcgatggt agacggtaac gcctacgagt tgctgctgga tctgttcgaa
     1261 accaaaatcg aacagttggc agatgaaatt gaaaatatct atagcgacct ggagcagttg
     1321 agccgggtga ttatggaagg gcatcagggc gatgagtacg acgaggcgct ctccacgctg
     1381 gcggaactgg aagatatcgg ctggaaagtt cgcctgtgtc tgatggatac ccagcgcgct
     1441 ctcaacttcc tggtgcgtaa agcgcgttta ccgggtgggc aactggagca ggcgcgtgaa
     1501 atcctgcgcg atatcgaatc cctgctgccg cataacgaat ccctgttcca gaaggtgaac
     1561 ttcctgatgc aggcggcaat gggttttatc aacatcgagc agaaccgcat catcaaaatt
     1621 ttctcggtgg tatccgtcgt gttcctgccg ccaacgctgg ttgcttccag ctacgggatg
     1681 aactttgagt ttatgccaga actgaagtgg agtttcggct accctggcgc gattatcttt
     1741 atgatcctcg cgggcctggc accgtatctg tactttaagc ggaagaactg gttgtaaaaa
     1801 gaaggcggtg gcttagcctg gctaagccac ctgttattca aaggctccag gtatttaacc
     1861 cttttacctc tttctcataa aaccatttgt tcgtgttaac agcaacatag gctgctacgg
     1921 caattcccag aatgttaacg ccaattaatg caccaacaaa tagagataga atgccaagta
     1981 atagaactat aattgctttt ttccataagc ccagaacaaa taaatagatc caggaacaga
     2041 agaaagcaat gaaattcatt tgaatagtta atcgttgtct tacttttaat gctttaaatg
     2101 ctgctttata ttctggtgtt gctccccaga atccaggaaa gccatgttga tcataaaaat
     2161 taaatcggta tttccatttt tcacttaatg aatcatcgtt catatattcc ttactcataa
     2221 atactccata actattgttt tgatgaatca gtaggtgcaa gcattagcat actgaaagtg
     2281 gaaaaataac aaatcaaaaa aatcatcgaa ccattgcctg aacaggcaaa atcttcggct
     2341 atcattgtga tgatagagat gatatatact gctaatgtac caaaaacata aatttttata
     2401 tatatgaaac cactatcacg gagtcgttgg caattcatgt tgatgacgag ataatggagt
     2461 gcgatggtag agactataac aagaaaacct gcttccccat cgttaaaaaa gataataaga
     2521 aaggcaaaaa tgaaatttat taaaataaat aaaaatatat atcggtgtct ggaaatctta
     2581 ccgttagatg ttgggataaa catacgtaac ataaatttta catccttgtg tgagtctcct
     2641 gtcagcatgg caatatgccc actctcatac aagagtcggc atattttttc agaatatatt
     2701 tattttttat ttggacgttc tacgctgcgt ataaatcgcg tccatcacaa aaattgccag
     2761 cgccacccaa ataaaggtga aagtcaccat cttatcggca cccggttttt caccataaaa
     2821 cgtcacagcc agcaggaaca tcagcgtcgg gccaatgtac tgaaaaaagc ctaacgttga
     2881 gagacgcagg cgcgtggcag cggcagtaaa acacaacagc ggtacggtag tgacaatacc
     2941 ggcggcgatc agcagtaaat tcagcgacat cgggttttgc cccatatggc tggttgaact
     3001 gtcggcaata gcaaacaggt aaatcgccgc cacgggcagc agccacatgg tttcaattaa
     3061 catgccggtt tgtgcttcta ccgcaatctt cttgcgcacc aggccgtaga aggcaaaact
     3121 aaatgccagc cccagcgcga taataggtag cgaaccaaaa gtccacagct ggaccaacac
     3181 gccacatatc gccagaatca ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa
     3241 aatcatcccc agcacaatgt tcaccagcgg gttaataaag taaccaaggc tcgcttccag
     3301 catatggtga ttgttcaccg cccagataaa cagtagccag ttgccaccaa tcagtacggc
     3361 agagactgcc agcataaaaa ttttctgtgg cgtctgaatc agcgttttta aataggacca
     3421 ctggcggcaa atgctcatca gcaccaccat aaagaaaaac gaccagatca cgcgatgcgt
     3481 caggatctca tcggcgggca cgtagtaaat cagcttgaag tatgctggcg caatacccca
     3541 aataaaataa gcggcaagag cgagtaatac gccctgccgc gtttgttttg catccatcgg
     3601 gaatactcat ttttaattgg taacagcagt ttacctgctt ttatgtcttc aacctaccat
     3661 ataggtggcg gtggcactgg caatataaag ctgttcttca ttgtgtaatt caacgcgggc
     3721 gacggcgact ttattgcctg cacgcaacag gctactggta gcagtaaaac gctcgcccct
     3781 gcctgggcgc agataatcaa cgcgaagatc aatggtcccc atccgcgata gccgctggcg
     3841 tagttcatct tcactgatgg tttcgtggcg ggttaaggta cttcccacgc acaccagacc
     3901 ggcggcgaca tccagcgccg acgcaatgac tccgccgtgc aaaatgcttt gcgcccagtt
     3961 acccaccatc atcggctgat ttttaaaggc cagctgtgcg aactcttttt cgtaacgctc
     4021 cagttccatc cccaatgcgc ggttaaatgg catgtgataa acaaacatct cacccactaa
     4081 tttcagggct tgttcagcgg tcagtacggc agacatatca tccttacact tcattggtta
     4141 atgaaatgtt gattttatgc ttctttgttg ttggtttcta ctttaggaag ggataactaa
     4201 cggcaatgga gttaagtatg taaaatagcc cgcagaaaaa cattcacctt atcaataatt
     4261 cgttacggag aacacgaccg atgcggactc tgcagggctg gttgttgccg gtgtttatgt
     4321 tgcctatggc agtatatgca caagaggcaa cggtgaaaga ggtgcatgac gcgccagcgg
     4381 tgcgtggcag tattatcgcc aatatgctgc aggagcatga caatccgttc acgctctatc
     4441 cttatgacac caactacctc atctacaccc aaaccagcga tctgaataaa gaagcgattg
     4501 ccagttacga ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag ttgagcctgg
     4561 catttccgct gtggcgtggg attttaggcc caaactcggt gttgggtgcg tcttatacgc
     4621 aaaaatcctg gtggcaactg tccaatagcg aagagtcttc accgtttcgt gaaaccaact
     4681 acgaaccgca attgttcctc ggttttgcca ccgattaccg ttttgcaggt tggacgctgc
     4741 gcgatgtgga gatggggtat aaccacgact ctaacgggcg ttcagacccg acctcccgca
     4801 gctggaaccg cctttatact cgcctgatgg cagaaaacgg taactggctg gtagaagtga
     4861 agccgtggta tgtggtgggt aatactgacg ataacccgga tatcaccaaa tatatgggtt
     4921 actaccagct taaaatcggc tatcacctcg gtgatgcggt gctcagtgcg aaaggacagt
     4981 acaactggaa caccggctac ggcggcgcgg agttaggctt aagttacccg atcaccaaac
     5041 atgtgcgcct ttatactcag gtttacagcg gctatggcga atcgctcatc gactataact
     5101 tcaaccagac ccgtgtcggt gtaggggtta tgctaaacga cttgttttga tgaacggttg
     5161 agtggttggc aaatctggaa tccagcatcc aggattaccc tctcagagac taaaagcatt
     5221 gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca atcggggtga
     5281 atgtggcgca ggcggaagtg ttgaatctgg agtccggagc taaacaggtt ttacaagaaa
     5341 cctttggcta ccaacagttt cgccccggcc aggaagaaat tatcgacact gtgctttccg
     5401 gtcgcgattg cctggttgtt atgcccactg gtggcggaaa atccctttgc tatcaaattc
     5461 ctgccttatt gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg ttgatgaaag
     5521 atcaggtgga tcaactacag gccaacggcg tggcggcggc gtgccttaac tcgacgcaaa
     5581 cccgcgaaca gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt cgcttactgt
     5641 atatcgcccc ggaacgcctg atgctggata actttcttga gcatctggcg cactggaatc
     5701 cggtgttatt agccgtcgat gaagcgcact gtatctccca atggggtcac gatttccgcc
     5761 cggaatatgc cgcgctcggt cagttgcgcc agcggttccc gacgctgccg tttatggcgc
     5821 tgaccgccac agccgacgac accacgcgcc aggatatcgt gcgcctgctg gggctgaacg
     5881 atccgctgat tcaaatcagc agttttgacc gtccgaatat tcgctacatg ctgatggaga
     5941 aattcaaacc gctcgatcag ttgatgcgct acgtgcagga acagcgcggt aagtctggca
     6001 ttatctactg caacagccgg gcgaaagtag aagacaccgc tgcgcgcctg caaagcaggg
     6061 gtattagcgc ggcggcctat catgccgggc tggaaaataa tgtccgcgcc gacgtgcagg
     6121 agaaattcca gcgcgatgat ctgcaaattg tggtggcgac ggtggcgttc ggcatgggca
     6181 tcaataaacc aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat atcgaatcct
     6241 attatcagga aaccggtcgc gccgggcgtg atggcctgcc tgcggaagcg atgctgtttt
     6301 acgatccggc tgatatggcg tggctgcgcc gttgtctgga agagaagccg caggggcagt
     6361 tgcaggatat cgagcgccac aaactcaatg cgatgggcgc gtttgccgaa gcgcaaactt
     6421 gccgtcgtct ggtattgctg aactattttg gcgaagggcg tcaggagccg tgcgggaact
     6481 gcgatatctg cctcgatccg ccgaaacagt acgacggttc aaccgatgct cagattgccc
     6541 tttccaccat tggtcgtgtg aatcagcggt ttgggatggg ttatgtggtg gaagtgattc
     6601 gtggtgctaa taaccagcgt atccgcgact atggtcatga caaactgaaa gtctatggta
     6661 tgggccgtga taaaagccat gaacattggg tgagcgtgat ccgccagctg attcatctcg
     6721 gcctggtgac gcaaaatatt gcccagcatt ctgccctaca actgacagag gccgcgcgcc
     6781 cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg gcgctcaaac
     6841 cgaaagcgat gcagaaatcg ttcggcggca actatgatcg caaactgttc gccaaattac
     6901 gcaaactgcg taaatcgatt gccgatgaaa gtaatgtccc gccgtacgtg gtgtttaacg
     6961 acgcaacctt gattgagatg gctgaacaga tgccgatcac cgccagcgaa atgctcagcg
     7021 ttaacggcgt tgggatgcgc aagctggaac gctttggtaa accgtttatg gcgctgattc
     7081 gtgcgcatgt cgatggcgac gacgaagagt agtcagcagc ataaaaaagt gccagtatga
     7141 agactccgta aacgtttccc ccgcgagtca aatgtatgtt gatgttattt ctcaccgtcg
     7201 ccatggtgca cattgtggcg cttatgagcc ccggtcccga tttctttttt gtctctcaga
     7261 ccgctgtcag tcgttcccgt aaagaagcga tgatgggcgt gctgggcatt acctgcggcg
     7321 taatggtttg ggctgggatt gcgttgcttg gcctgcattt gattatcgaa aaaatggcct
     7381 ggctgcatac gctgattatg gtaggcggtg gcctgtatct ctgctggatg ggttaccaga
     7441 tgctacgcgg tgcactgaaa aaagaggctg tatctgcgcc tgcaccacag gttgagctgg
     7501 cgaaaagcgg gcgcagtttc ctgaaaggct tactgaccaa tctcgccaat ccgaaagcga
     7561 ttatctattt tggttcggta ttctcattgt ttgtcggcga taacgttggc actaccgagc
     7621 gctggggcat tttcgcgttg attattgtcg aaacactggc gtggtttacc gtcgttgcca
     7681 gtctgtttgc cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag tggattgatg
     7741 gttttgccgg ggcgttattt gccggatttg gcattcattt gattatttcg cggtgatgcc
     7801 agacgcgtct tcagagtaag tcggataagg cgtttacgcc gcatccgaca ttatttttca
     7861 cgcatgcctc gccgatgcta acagcgctcc caccagcata aacaacgagc cgaaaatctt
     7921 attcagcgcc ttcatctgct ttggtccttt aatccataga gcaatccttt gagcaagggt
     7981 ggcgtaaccg atcatcacaa taatatcgac cacaatagtg gtgacgccga gcacgatata
     8041 ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga aatagcgccg ccagaaacac
     8101 aatacttttg ggattggtga gattcacaaa aactgcgcgc tggaacaaat gtcgacgcga
     8161 ttgagtagag gccagcgatt taagatcaat tgcgccagcg gcgcgccact gctggattcc
     8221 cagccaaatc aagtaagccg cgcctgccca ctttaacact tcaaacgcaa tcactgagcg
     8281 ggaaaatagc gtccccaacc ccacgccaac cagcacaata tgaatcgcca gtccggtctg
     8341 aagcccagca atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg tggtcatagt
     8401 gttgattgca ccagagcctg gcgacaggct taaaatgatc gatgtcagca ggtaggcaaa
     8461 ccaccattct aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca caatacgcta
     8521 ctgtcgcagc gttgtgtcag gcacgcttaa aaaaacgatt ttacgtggtt taagaggcag
     8581 attacccgat gtttcagcag caaaaagact gggaaacaag agaaaacgcg tttgctgctt
     8641 ttaccatggg accgctgact gatttctggc gtcagcgtga tgaagcagag tttactggtg
     8701 tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac cgggtggtag
     8761 tcatctgccc ggggcgtatt gagagctacg taaaatatgc ggaactggcc tacgacctgt
     8821 tccatttggg gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt tccggtcgcc
     8881 tgttagccga tccgcatctc gggcatgtta atcgctttaa tgattatgtt gatgatctgg
     8941 cggcattctg gcagcaggag gttcagcccg gtccgtggcg taaacgctat atactggcac
     9001 attcgatggg cggtgcgatc tccacattat ttctgcaacg ccatccaggt gtatgtgacg
     9061 ccattgcgct aactgcgcca atgtttggga tcgtgattcg tatgccgtca tttatggcac
     9121 ggcagatcct caactgggcc gaagcgcatc cacgtttccg tgatggctat gcaataggca
     9181 ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc agacagcgat
     9241 atcgacgtaa cttacgcttc tatgctgatg acccaacgat tcgcgtcggt gggccgacct
     9301 accattgggt acgcgaaagt attctggctg gcgaacaggt gttagccggt gcgggtgatg
     9361 acgccacgca aacgcttctc ttacaagctg aagaggaacg cgtggtggat aaccgcatgc
     9421 acgaccgttt ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga ggacggccgt
     9481 tggtaataaa aggtgcttac catgagatcc tttttgaaaa ggacgcaatg cgctcagtcg
     9541 cgctccacgc catcgttgat tttttcaaca ggcataactc acccagcgga aaccgctcta
     9601 cagaggttta aatttcttat gtaccaggtt gttgcgtctg atttagatgg cacgttactt
     9661 tctcccgacc atacgttatc cccttacgcc aaagaaactc tgaagctgct caccgcgcgc
     9721 ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg atgtggggca aattcgcgat
     9781 aatctggaga ttaagtctta catgattacc tccaatggtg cgcgcgttca cgatctggat
     9841 ggcaatctga tttttgctca taacctggat cgcgacattg ccagcgatct gtttggcgta
     9901 gtcaacgaca atccggacat cattactaac gtttatcgcg acgacgaatg gtttatgaat
     9961 cgccatcgcc cggaagagat gcgctttttt aaagaagcgg tgttcaaata tgcgctgtat
    10021 gagccgggat tactggagcc ggaaggcatc agcaaagtgt ttttcacctg cgattcccat
    10081 gaacaactgc tgccgctgga gcaggcgatt aacgctcgct ggggcgatcg cgtcaacgtc
    10141 agtttctcta ccttaacctg tctggaagtg atggcgggcg gcgtgtcgaa aggccatgcg
    10201 ctggaagcag tggcgaagaa actgggctac agcctgaagg attgtattgc gtttggtgac
    10261 gggatgaacg acgccgaaat gctgtcgatg gcggggaaag gctgcattat gggcagtgcg
    10321 caccagcgtc tgaaagatct gcatccggag ctggaagtta ttggtactaa tgccgacgac
    10381 gcggtgccgc attatctgcg taaactctat ttatcgtaat cgttctttat ttggtcagtt
    10441 gtcaacctga tacttcgcta caatggatac tcgttaatca aagagttttc cattgtggcg
    10501 ttacttatca tcaccacgat tctgtgggcc ttctccttta gcttttatgg cgagtacctt
    10561 gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg ttggcttggc ggcactcgtt
    10621 tttctgccgt ttctgcgtac ccgtagcaat agcctgaaaa cggtcggcct gtatatgctg
    10681 gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt tccgcgctta tctctacctg
    10741 acggtctccg aattgttgct gttcaccgta ctgacgccgc tctacatcac gctgatttat
    10801 gacatcatga gtaagcgccg tctgcgctgg ggctatgctt ttagcgcctt gctggcggtg
    10861 attggtgccg ggattattcg ctatgatcag gtcaccgacc atttctggac tggcttgttg
    10921 ctggtgcaac tctccaatat cacttttgcc atcggcatgg tgggttacaa acgcctgatg
    10981 gaaactcgcc cgatgccgca gtataacgcc tttgcgtggt tctatcttgg cgcgttcctg
    11041 gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga aaatgccgca aaccacgttg
    11101 caatggggca ttctggtgtt tcttggcgtg gtggcttccg ggattggcta ctttatgtgg
    11161 aactacggcg cgacgcaggt ggacgccgga acgctgggca ttatgaataa tatgcacgtt
    11221 ccggcagggc tgctggtaaa cctggctatc tggcaccaac agccgcactg gccaacgttt
    11281 attacaggcg cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg ggtcgctccg
    11341 cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga ataaacgctt
    11401 ccgtaactgg ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg ctccacaagc
    11461 cttcgcccag ggttttggtc accaccagac cctggcgctc aaaactctct actacccaat
    11521 gcggtagcgc ggcaataccc atccgcgcgg caaccatttg aatcaacaat aaggtgttat
    11581 cgacgctttt cagtgacggg ctgacgcctg ccggctgaag aaaatgccgc cagacatcca
    11641 gtcgactacg ctgcaccgga taaattaata gcgtctcgct ggcgagatct tccggtgtaa
    11701 tacgcgtttt tgccgccagt ggatggtcag gtgctaacac caggcgtact tcataatcga
    11761 acatcggcga ataatgcagg ccactgcgcg gcaggatatc ggaggtcatc accagatcca
    11821 gctcgccctg ttgcaacgcg ggctgcgggt caaacgtcac gcccgattta aaatccatct
    11881 ctacctgcgg ccagttctta tggaaatttc ctaaagcggg tgtcagccac tgaatacagc
    11941 tatggcactc aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag gcttgcagtg
    12001 cctggctgat ttgcggcagc acctggtttg ccagttgcaa caggatttct ccctgcggtg
    12061 taaagcgtag cggctggctc ttacgcacaa atagccggaa gccaaggcgt tgttccagat
    12121 cgctaaactg gtgagacagg gcggattgcg tctgatgcaa cgttgccgca gcggctgcga
    12181 gcgagccgca gttccgcaac gcttgtagcg ttttcaggtg ttttacttcg atcatgaaag
    12241 tccttcactt cggcatgaat aatttgcgct tgaggaatat acagtaaccg ccaattatgg
    12301 atgtgtaaac atctggacgg ctaaaatcat tcgtctttta aatttatggt gcgttggctg
    12361 cgtttctcca ccccggtcac ttacttcagt aagctcccgg cgatgaataa acttgccgcc
    12421 ttccctaaat tcaaaatcca taggatttac atataattag aggaagaaaa atgacaatat
    12481 tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag
    12541 aaagttattg ggcggggaac tccacgcgtg aagaactgct gacagtaggg cgtgaactgc
    12601 gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga cctgctgccg gtgggcgatt
    12661 ttgcctggta cgatcatgta ctgaccacca gtttgctgct gggtaatgtt ccgccacgtc
    12721 atcagaacaa agacggttcg gtagatatcg acaccctgtt ccgtattggt cgtggacgtg
    12781 cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac accaactatc
    12841 actacatggt gccggagttc gttaaaggcc aacagttcaa actgacttgg acgcagctgc
    12901 tggaggaagt ggacgaggcg ctggcgctga gccacaacgt taagcctgtg ctgctggggc
    12961 cggttactta cctgtggctg gggaaagtga aaggtgaaca gtttgaccgc cttagcctgc
    13021 tgaacgacat tctgccggtt tatcagcaag tgctggcaga actggcgaaa cgcggcatcg
    13081 agtgggtaca gattgatgaa cccgcgctgg tactggaact accacaggcg tggctggacg
    13141 catacaaacc cgcttacgac gcgctccagg gacaggtgaa actgctgctg accacctatt
    13201 ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct gcctgttcag gggctgcatg
    13261 tggaccttgt acatggtaaa gataacgttg ttgaactgca caagcgcctg ccttctgact
    13321 ggctgctgtc tgcgggtctg atcaatggtc gtaacgtctg gcgcgccgat cttactgaga
    13381 aatatgcgca aattaaggac attgtcggca aacgtgattt gtgggtggca tcttcctgct
    13441 cgttgctgca cagccccatc gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa
    13501 gctggtttgc cttcgcccta caaaaatgcc atgaactggc actgctgcgc gatgcgctga
    13561 acagtggtga cacggcagct ctggcagagt ggagcgcccc aattcaggcg cgtcgtcact
    13621 ctactcgtgt acataatccg gcggtagaaa agcgtctggc ggcgatcacc gcccaggaca
    13681 gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt tttaaactgc
    13741 cagcgtggcc gaccaccacg attggttcct tcccgcaaac cacggaaatt cgtaccctgc
    13801 gtctggattt caaaaagggc aatctcgacg ccaacaacta ccgcacgggc attgcggaac
    13861 atatcaagca ggccattgtt gagcaggaac gtttgggact ggatgtgctg gtacatggcg
    13921 aggccgagcg taatgacatg gtggaatact ttggcgagca cctcgacgga tttgtcttta
    13981 cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt gaagccaccg attgtcattg
    14041 gtgacattag ccgcccggca ccgattaccg tggagtgggc gaagtatgcg caatcgctga
    14101 ccgacaaacc ggtgaaaggg atgctgacgg ggccggtgac catactctgc tggtcgttcc
    14161 cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat tgcgctggcg ctgcgtgatg
    14221 aagtggccga tctggaagcc gctggaattg gcatcatcca gattgacgaa ccggcgctgc
    14281 gcgaaggttt accgctgcgt cgtagcgact gggatgcgta tctccagtgg ggcgtagagg
    14341 ccttccgtat caacgccgcc gtggcgaaag atgacacaca aatccacact cacatgtgtt
    14401 attgcgagtt caacgacatc atggattcga ttgcggcgct ggacgcagac gtcatcacca
    14461 tcgaaacctc gcgttccgac atggagttgc tggagtcgtt tgaagagttt gattatccaa
    14521 atgaaatcgg tcctggcgtc tatgacattc actcgccaaa cgtaccgagc gtggaatgga
    14581 ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc agagcgcctg tgggtcaacc
    14641 cggactgtgg cctgaaaacg cgcggctggc cagaaacccg cgcggcactg gcgaacatgg
    14701 tgcaggcggc gcagaatttg cgtcgggggt aaatccaaac cgggtggtaa taccacccgg
    14761 tcttttctca ttacagcgac ttcttcccac catactgctt aaaccattcc agcatacgct
    14821 gccagccatc tttcgcagat tcggcatgat agctcgggcg ataatcagcg ttgaatgcat
    14881 gcccggcatc cgggtacacg ataatctctg ctttcgcatt agcagcccgc agcgcctggc
    14941 gcatcgtttc aacgctctcc tgcggaatgc tgttatcctg accaccatat aagccgagaa
    15001 ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt cggtgaattc agcgacttgt
    15061 cgcccgtcag tttgccgtac cacgccactg cggcttttag ctgtggatta tgcgcggcat
    15121 acagccaggt gatacgtcca ccccagcaga atccggtgat cattaaacga tgaacatcgc
    15181 cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc ggccagcacc tgcgagtcag
    15241 gcacttttgc taccagaccg ctaagcaacg tggggatatc ggcaaaatca ttcggatcgc
    15301 cttcgcggaa gtaaagttca ggtgcgatag ccagataccc ctccagcgcc agacggcgac
    15361 aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg cactacaatg accactggca
    15421 gtgggccatc gctttgcttt ggtctggcat ggtaagcagg catgttatcc ccttgtgaag
    15481 ggatagaggt gaagcccgcc acaatcgcgt cgtccggggt ctgaacgatg gtcgaagcga
    15541 gaggcgatgc agcaggtgca aatccagatt gttgtgttgt tgccatggta ttctccgtac
    15601 ccttataaaa atgttgcgca atgttaacta tagtcagcat gcaacaaatc acattgcctg
    15661 aatcggctca tcttttatgc agtcctgcag aatgaagagt gatttatgtg atatgaatca
    15721 cttttggtgg gtaaatttat gcaacgcatt tgcgtcatgg tgatgagtat cacgaaaaaa
    15781 tgttaaaccc ttcggtaaag tgtctttttg cttcttctga ctaaaccgat tcacagagga
    15841 gttgtatatg tccaagtctg atgtttttca tctcggcctc actaaaaacg atttacaagg
    15901 ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg ccgcgctgat
    15961 ggataagccg gttaagctgg catctcaccg cgaattcact acctggcgtg cagagctgga
    16021 tggtaaacct gttatcgtct gctctaccgg tatcggcggc ccgtctacct ctattgctgt
    16081 tgaagagctg gcacagctgg gcattcgcac cttcctgcgt atcggtacaa cgggcgctat
    16141 tcagccgcat attaatgtgg gtgatgtcct ggttaccacg gcgtctgtcc gtctggatgg
    16201 cgcgagcctg cacttcgcac cgctggaatt cccggctgtc gctgatttcg aatgtacgac
    16261 tgcgctggtt gaagctgcga aatctattgg cgcgacaact cacgttggcg tgacagtttc
    16321 ttctgatacc ttctacccag gtcaggaacg ttacgatact tactctggtc gcgtagttcg
    16381 tcactttaaa ggttctatgg aagagtggca ggcgatgggc gtaatgaact atgaaatgga
    16441 atctgcaact ctgctgacca tgtgtgcaag tcagggcctg cgtgccggta tggtagcggg
    16501 tgttatcgtt aaccgcaccc agcaagagat cccgaatgct gagacgatga aacaaaccga
    16561 aagccatgcg gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgtaat tctcttctcc
    16621 tgtctgaagg ccgacgcgtt cggccttttg tatttttgcg tagcgcctcg caggaaatgc
    16681 ctttccaact ggacgtttgt acagcacaat tctattttgt gcgggtaagt tgttgcgtca
    16741 ggaggcgttg tggatttctc aatcatggtt tacgcagtta ttgcgttggt gggtgtggca
    16801 attggctggc tgtttgccag ttatcaacat gcgcagcaaa aagccgagca attagctgaa
    16861 cgtgaagaga tggtcgcgga gttaagcgcg gcaaaacaac aaattaccca aagcgagcac
    16921 tggcgtgcag agtgtgaatt gctcaataac gaagtgcgca gcctgcaaag tattaacacc
    16981 tctctggagg ccgatctgcg tgaagtaacc acgcggatgg aagccgcaca gcaacatgct
    17041 gacgataaaa ttcgccagat gattaacagc gagcagcgcc tcagtgagca gtttgaaaac
    17101 ctcgccaacc gtatttttga gcacagcaat cgccgggttg atgagcaaaa ccgtcagagt
    17161 ctgaacagcc tgttgtcgcc gctacgtgaa caactggacg gtttccgccg tcaggttcag
    17221 gacagcttcg gtaaagaagc acaagaacgc cataccctga cccacgaaat tcgcaatctc
    17281 cagcaactca acgcgcaaat ggcccaggaa gcgatcaacc tgacgcgcgc gctgaaaggc
    17341 gacaataaaa cccagggcaa ctggggcgag gtagtattga cgcgggtgct ggaggcttcc
    17401 ggtctgcgtg aagggtatga atatgaaacc caggtcagca tcgaaaatga cgcccgctcg
    17461 cggatgcagc cggatgtcat cgtgcggctg ccgcagggaa aagatgtggt gatcgacgcc
    17521 aaaatgacgc tggtcgccta tgaacgctat tttaacgccg aagacgacta cacccgcgaa
    17581 agcgcgctac aggaacatat cgcgtcggtg cgtaaccata tccgtttgct gggacgcaaa
    17641 gattatcaac agctgccggg gctgcgaact ctggattacg tgctgatgtt tattcccgtt
    17701 gaacccgctt ttttactggc gcttgaccgc cagccggagc tgatcaccga agcgttgaaa
    17761 aacaatatca tgctggttag cccgactacg ctgctggtgg cgttgcgtac tattgccaac
    17821 ctgtggcgtt atgagcatca aagccgtaac gcccagcaaa tcgccgatcg tgccagcaag
    17881 ctgtacgaca agatgcgttt gttcatcgat gacatgtccg cgattggtca aagtctcgac
    17941 aaagcgcagg ataactaccg ccaggcaatg aaaaaactct cttcaggtcg cggaaatgtg
    18001 ctggcgcagg cagaagcgtt tcgcggttta ggagtagaaa ttaaacgcga gattaatccg
    18061 gatttggctg aacaggcggt gagccaggat gaagagtatc gacttcggtc ggttccggag
    18121 cagccgaatg atgaagctta tcaacgcgat gatgaatata atcagcagtc gcgctagccc
    18181 attaggggta gttaagccgg gtagaaatct agggcatcga cgcccaatct gttacacttc
    18241 tggaacaatt ttttgatgag caggcattga gatggtggat aagtcacaag aaacgacgca
    18301 ctttggtttt cagaccgtcg cgaaggaaca aaaagcggat atggtcgccc acgttttcca
    18361 ttccgtggca tcaaaatacg atgtcatgaa tgatttgatg tcatttggta ttcatcgttt
    18421 gtggaagcga ttcacgattg attgcagcgg cgtacgccgt gggcagaccg tgctggatct
    18481 ggctggtggc accggcgacc tgacagcgaa attctcccgc ctggtcggag aaactggcaa
    18541 agtggtcctt gctgatatca atgaatccat gctcaaaatg ggacgcgaga agctgcgtaa
    18601 tatcggtgtg attggcaacg ttgagtatgt tcaggcgaac gctgaggcgc tgccgttccc
    18661 ggataacacc tttgattgca tcaccatttc gtttggtctg cgtaacgtca ccgacaaaga
    18721 taaagcactg cgttcaatgt atcgcgtgct gaaacccggc ggccgcctgc tggtgcttga
    18781 gttctcgaag ccaattatcg agccgctgag caaagcctat gatgcatact ccttccatgt
    18841 gctgccgcgt attggctcac tggtcgcgaa cgacgccgac agctaccgtt atctggcaga
    18901 atccatccgt atgcatcccg atcaggatac cctgaaagcc atgatgcagg atgccggatt
    18961 cgaaagtgtc gactactaca atctgacggc aggggttgtg gcgctgcatc gtggttataa
    19021 gttctgacag gagaccggaa atgcctttta aacctttagt gacggcagga attgaaagtc
    19081 tgctcaacac cttcctgtat cgctcacccg cgctgaaaac agcccgctcg cgtctgctgg
    19141 gtaaagtatt gcgcgtggag gtaaaaggct tttcgacgtc attgattctg gtgttcagcg
    19201 aacgccaggt tgatgtactg ggcgaatggg caggcgatgc tgactgcacc gttatcgcct
    19261 acgccagtgt gttgccgaaa cttcgcgatc gccagcagct taccgcactg attcgcagtg
    19321 gtgagctgga agtgcagggc gatattcagg tggtgcaaaa cttcgttgcg ctggcagatc
    19381 tggcagagtt cgaccctgcg gaactgctgg ccccttatac cggtgatatc gccgctgaag
    19441 gaatcagcaa agccatgcgc ggaggcgcaa agttcctgca tcacggcatt aagcgccagc
    19501 aacgttatgt ggcggaagcc attactgaag agtggcgtat ggcacccggt ccgcttgaag
    19561 tggcctggtt tgcggaagag acggctgccg tcgagcgtgc tgttgatgcc ctgaccaaac
    19621 ggctggaaaa actggaggct aaatgacgcc aggtgaagta cggcgcctat atttcatcat
    19681 tcgcactttt ttaagctacg gacttgatga actgatcccc aaaatgcgta tcaccctgcc
    19741 gctacggcta tggcgatact cattattctg gatgccaaat cggcataaag acaaactttt
    19801 aggtgagcga ctacgactgg ccctgcaaga actggggccg gtttggatca agttcgggca
    19861 aatgttatca acccgccgcg atctttttcc accgcatatt gccgatcagc tggcgttatt
    19921 gcaggacaaa gttgctccgt ttgatggcaa gctggcgaag cagcagattg aagctgcaat
    19981 gggcggcttg ccggtagaag cgtggtttga cgattttgaa atcaagccgc tggcttctgc
    20041 ttctatcgcc caggttcata ccgcgcgatt gaaatcgaat ggtaaagagg tggtgattaa
    20101 agtcatccgc ccggatattt tgccggtcat taaagcggat ctgaaactta tctaccgtct
    20161 ggctcgctgg gtgccgcgtt tgctgccgga tggtcgccgt ctgcgcccaa ccgaagtggt
    20221 gcgcgagtac gaaaagacct tgattgatga actgaatttg ctgcgggaat ctgccaacgc
    20281 cattcagctt cggcgcaatt ttgaagacag cccgatgctc tacatcccgg aagtttaccc
    20341 tgactattgt agtgaaggga tgatggtgat ggagcgcatt tacggcattc cggtgtctga
    20401 tgttgcggcg ctggagaaaa acggcactaa catgaaattg ctggcggaac gcggcgtgca
    20461 ggtgttcttc actcaggtct ttcgcgacag ctttttccat gccgatatgc accctggcaa
    20521 catcttcgta agctatgaac acccggaaaa cccgaaatat atcggcattg attgcgggat
    20581 tgttggctcg ctaaacaaag aagataaacg ctatctggca gaaaacttta tcgccttctt
    20641 taatcgcgac tatcgcaaag tggcagagct acacgtcgat tctggctggg tgccaccaga
    20701 taccaacgtt gaagagttcg aatttgccat tcgtacggtc tgtgaaccta tctttgagaa
    20761 accgctggcc gaaatttcgt ttggacatgt actgttaaat ctgtttaata cggcgcgtcg
    20821 cttcaatatg gaagtgcagc cgcaactggt gttactccag aaaaccctgc tctatgtcga
    20881 aggggtagga cgccagcttt atccgcagct cgatttatgg aaaacagcga agcctttcct
    20941 ggagtcgtgg attaaagatc aggtcggtat tcctgctctg gtgagagcat ttaaagaaaa
    21001 agcgccgttc tgggtcgaaa aaatgccaga actgcctgaa ctggtttacg acagtttgcg
    21061 ccagggcaag tatttacagc acagtgttga taagattgcc cgcgagcttc agtcaaatca
    21121 tgtacgtcag ggacaatcgc gttattttct cggaattggc gctacgttag tattaagtgg
    21181 cacattcttg ttggtcagcc gacctgagtg ggggctgatg cccggctggt taatggcagg
    21241 tggtctgatc gcctggtttg tcggttggcg caaaacacgc tgattttttc atcgctcaag
    21301 gcgggccgtg taacgtataa tgcggctttg tttaatcatc atctaccaca gaggaacatg
    21361 tatgggtggt atcagtattt ggcagttatt gattattgcc gtcatcgttg tactgctttt
    21421 tggcaccaaa aagctcggct ccatcggttc cgatcttggt gcgtcgatca aaggctttaa
    21481 aaaagcaatg agcgatgatg aaccaaagca ggataaaacc agtcaggatg ctgattttac
    21541 tgcgaaaact atcgccgata agcaggcgga tacgaatcag gaacaggcta aaacagaaga
    21601 cgcgaagcgc cacgataaag agcaggtgta atccgtgttt gatatcggtt ttagcgaact
    21661 gctattggtg ttcatcatcg gcctcgtcgt tctggggccg caacgactgc ctgtggcggt
    21721 aaaaacggta gcgggctgga ttcgcgcgtt gcgttcactg gcgacaacgg tgcagaacga
    21781 actgacccag gagttaaaac tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc
    21841 gagcctcact aacctgacgc ccgaactgaa agcgtcgatg gatgaactac gccaggccgc
    21901 ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca
    21961 caccatccat aacccggtgg tgaaagataa tgaagctgcg catgagggcg taacgcctgc
    22021 cgctgcacaa acgcaggcca gttcgccgga acagaagcca gaaaccacgc cagagccggt
    22081 ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga
    22141 taaaccgtaa acatgtctgt agaagatact caaccgctta tcacgcatct gattgagctg
    22201 cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga tattcctgtg tctggtctat
    22261 ttcgccaatg acatctatca cttggtatcc gcgccattga tcaagcagtt gccgcaaggt
    22321 tcaacgatga tcgccaccga cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt
    22381 atggtgtcgc tgattctgtc agcgccggtg attctctatc aggtgtgggc atttatcgcc
    22441 ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc tgctggtttc cagctctctg
    22501 ctgttttata tcggcatggc attcgcctac tttgtggtct ttccgctggc atttggcttc
    22561 cttgccaata ccgcgccgga aggggtgcag gtatccaccg acatcgccag ctatttaagc
    22621 ttcgttatgg cgctgtttat ggcgtttggt gtctcctttg aagtgccggt ggcaattgtg
    22681 ctgctgtgct ggatggggat tacctcgtca gaagacttac gcaaaaaacg cccgtatgtg
    22741 ctggttggtg cattcgttgt cgggatgttg ctgacgccgc cggatgtctt ctcgcaaacg
    22801 ctattggcga tcccgatgta ctgtctgttt gaaatcggtg tcttcttctc acgcttttac
    22861 gttggtaaag ggcgaaatcg ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact
    22921 gaagaataaa ttcaaccgcc cgtcagggcg gttgtcatat ggagtacagg atgtttgata
    22981 tcggcgttaa tttgaccagt tcgcaatttg cgaaagaccg tgatgatgtt gtagcgcgcg
    23041 cttttgacgc gggagttaat gggctactca tcaccggcac taacctgcgt gaaagccagc
    23101 aggcgcaaaa gctggcgcgt cagtattcgt cctgttggtc aacggcgggc gtacatcctc
    23161 acgacagcag ccagtggcaa gctgcgactg aagaagcgat tattgagctg gccgcgcagc
    23221 cagaagtggt ggcgattggt gaatgtggtc tcgactttaa ccgtaacttt tcgacgccgg
    23281 aagagcagga acgcgctttt gttgctcagc tacgcattgc cgcagaatta aacatgccgg
    23341 tatttatgca ctgtcgcgat gcccacgagc ggtttatgac attgctggag ccgtggctgg
    23401 ataaactgcc tggtgcggtt cttcattgct ttaccggcac acgcgaagag atgcaggcgt
    23461 gcgtggcgcg tggaatttat atcggcatta ccggttgggt ttgcgatgaa cgacgcgggc
    23521 tggagctgcg ggaattgttg ccgttgattc cggcggagaa attgctgatc gaaacagatg
    23581 cgccgtatct gctcccgcgc gatctcacgc caaagccatc atcccggcgc aacgagccag
    23641 cccatctgcc ccatattttg caacgtattg cgcactggcg tggagaagat gccgcatggc
    23701 tggctgccac cacggatgcc aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc
    23761 ggaactcggt gttcttcaca ctgtgcttaa tctctttatt aataagatta agcaatagca
    23821 tggagcgagc ctcaccatcg ggttcggtga aaatggcctg aaagccttcg aacgcgcctt
    23881 cggtaataat caccttatca cccggataag ggggggccgg atcgacaatg tctttcggtt
    23941 tatataccga tagctgatga attaccgccg atgggactat cgctggcgac gcgccaaagc
    24001 gcacaaagtg gctgacgcca cgcgtagcgc tgatggtcgt ggtatgaatc acttctggat
    24061 caaattccac aaacaggtag ttagggaata acggttcact gactgcggta cgtttacccc
    24121 gtacgatttt ttccagggtg atcatcggag cgaggcaatt aaccgcctgt ctctcaaggt
    24181 gttcctgggc acgttgaagt tgcccgcgct tgcagtacag taaataccag gattgcataa
    24241 tgactcttat ccgtttaatc ggggcgcaag gatagcaaaa gctttacgct aagttaatta
    24301 tattccccgg tttgcgttat accgtcagag ttcacgctaa tttaacaaat ttacagcatt
    24361 gcaaagatga acgccgtata atgggcgcag attaagaggc tacaatggac gccatgaaat
    24421 ataacgattt acgcgacttc ctgacgctgc ttgaacagca gggtgagcta aaacgtatca
    24481 cgctcccggt ggatccgcat ctggaaatca ctgaaattgc tgaccgcact ctgcgtgctg
    24541 gtgggcctgc gctgttgttc gaaaacccta aaggctactc aatgccggtg ctgtgcaacc
    24601 tgttcggtac gccaaagcgc gtggcgatgg gcatggggca ggaagatgtt tcggcgctgc
    24661 gtgaagttgg taaattattg gcgtttctga aagagccgga gccgccaaaa ggtttccgcg
    24721 acctgtttga taaactgccg cagtttaagc aggtgttaaa catgccgaca aagcgactgc
    24781 gtggtgcgcc ctgccaacaa aaaatcgtct ctggcgatga cgtcgatctc aatcgcattc
    24841 ccattatgac ctgctggccg gaagatgccg cgccgctgat tacctggggg ctgaccgtaa
    24901 cgcgcggccc gcataaagag cggcagaatc tgggcattta tcgccagcag ctgattggta
    24961 aaaacaaact gattatgcgc tggctgtcgc atcgcggcgg cgcgctggat tatcaggagt
    25021 ggtgtgcggc gcatccgggc gaacgtttcc cggtttctgt ggcgctgggt gccgatcctg
    25081 ccacgattct cggtgcagtc acccccgttc cggatacgct ttcagagtat gcgtttgccg
    25141 gattgctacg cggcaccaaa accgaagtag taaagtgtat ttccaatgac ctcgaagtgc
    25201 cagccagtgc cgaaatcgtc ctggaagggt atatcgatcc tggtgagatg gcgccggaag
    25261 ggccgtatgg tgaccacaca ggttactata atgaagtcga taatttcccg gtgtttaccg
    25321 tgacgcatat tacccagcgt gaagatgcga tttaccattc cacctatacc gggcgtccgc
    25381 cagatgagcc cgcggtactg ggcgtggcgt tgaacgaagt gtttgtaccg attctgcaaa
    25441 aacagttccc ggaaattgtc gatttttacc tgccgccgga aggctgttct tatcgtctgg
    25501 cggtagtgac gatcaaaaaa cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct
    25561 ggtcgttctt acgccagttt atgtacacta aatttgtgat cgtttgcgat gatgacgtca
    25621 acgcacgcga ctggaacgat gtgatttggg cgattaccac ccgtatggac ccggcgcggg
    25681 atactgttct ggtagaaaat acgcctattg attatctgga ttttgcctcg cctgtctctg
    25741 ggctgggttc aaaaatgggg ctggatgcca cgaataaatg gccgggggaa acccagcgtg
    25801 aatggggacg tcccatcaaa aaagatccag atgttgtcgc gcatattgac gccatctggg
    25861 atgaactggc tatttttaac aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt
    25921 tatcgatccg acagagaaag cgcatgacaa ccttaagctg taaagtgacc tcggtagcag
    25981 ctatcacgga taccgtatat cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg
    26041 ctggtcagta tttgatggta gtgatggatg agcgcgacaa acgtccgttc tcaatggcct
    26101 cgacgccgga tgaaaaaggg tttatcgagc tgcatattgg cgcttctgaa atcaaccttt
    26161 acgcgaaagc agtcatggac cgcatcctca aagatcatca aatcgtggtc gaccttcccc
    26221 acggagaagc gtggctgcgc gatgatgaag agcgtccgat gattttgatt gcgggcggca
    26281 ccgggttctc ttatgcccgc tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg
    26341 atatcaccat ttactggggc gggcgtgaag agcagcatct gtatgatctc tgcgagcttg
    26401 aggcgctttc gttaaagcac cttggtctgc aagtggtgcc ggtggttgaa caaccggaag
    26461 caggctggcg tgggcgtact ggtaccgtgt taacggcggt attgcaggat catggtacgc
    26521 tggcagagca tgatatctat attgccggac gttttgagat ggcgaaaatt gcccgtgacc
    26581 tgttttgcag tgagcgtaat gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta
    26641 tctgagatat aaaaaaaccc gcccctgaca ggcgggaaga acggcaacta aaactttatt
    26701 cagtggcatt tagatctatg tcgcatccgg caattggtgc acgatgcctg atgcgacgct
    26761 ggcgcgtctt atcaggccta cattggtgcc ggatcggtag accggataag gcgttcacgc
    26821 cgcatccggc aagtggttaa acccgctcaa acaccgtcgc aataccctga cccagaccga
    26881 tacacatcgt cgccagacca aactgaacgt ctttgcgttc catcagattc agcagcgtgg
    26941 tgctgatacg cgcaccggaa caacccagcg gatgacccag cgcgatcgca ccaccattga
    27001 ggttgatctt ctcgtcaatc tgctccatta gcccgagatc tttaatgcac ggcaggatct
    27061 gcgcggcaaa ggcttcgttc atctcaaaca cgccgatatc gctggcagaa agccccgctt
    27121 ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc cataatcgat gggtcacaac
    27181 caacgaccgc catcgaacgc acacgagcgc gcggcttaag acctaattca tgggcgcggc
    27241 tttcactcat caccagcatg gcagctgcgc catcggaaag tgcagaagat gtgcccgccg
    27301 ttaccgtacc gtttactgga tcaaacgccg gacgcagcgt ggcgagggct tccacggtgg
    27361 tttccgggcg aatcacttcg tcgtaattaa actgcttcag gacgccgtcg gcatcgtgac
    27421 caccagttgg gatgatttca tttttaaatg cggccgactg cgtggcggcc caggcgcggg
    27481 cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct gataccgtgc atacgcgcca
    27541 gcatttctgc cgttaagccc atcatgcccg ccgctttggc gacattgcgg ctcaggccgg
    27601 ggtgaaaatc gacgccgtga ctcatcggca catggcctat atgctccacg ccgccaacca
    27661 gacatgcctg cgtatcgcca gtcatgatca ttcgtgctgc gtcatgcagt gcctgcatgg
    27721 atgaaccaca caagcgattg acggtaaccg ccgggacaga gtgcggcact tctgccagca
    27781 gcgccgcgtt acgggcgatg ttaaaaccct gctccagcgt ctgttgcaca caaccccagt
    27841 aaatatcgtc gagggccgcc gcttccagcg ccgggttacg cgccagcagg ctacgcatta
    27901 aatgagcgga gagatcttcc gcacgcacgt tacgaaaagc accgcccttc gaacggccca
    27961 tcggggtgcg aattgcatcg acaatgacaa cctgttccat tgtgactcct taagccgttt
    28021 tcaggtcgcc aaccggacgg gctggctcaa ccggaggata gtacggttcg ttatgacgcg
    28081 ctttattacg cagaccttcc ggcacttcat acagcgggcc gaggtgctga tattgctgtg
    28141 ccatatcgag gtattttgcg ctaccgaggg tgtccagcca gcggaacgcg ccgccgtgga
    28201 acggagggaa gcccaggccg tagaccagcg ccatatccgc ttccgccgga gtggcgataa
    28261 tgccctcttc cagacagcgc accacttcgt tgaccatcgg gatcatcatg cgggcgataa
    28321 tctcttcttc gctgaaatcg cgcttcggtt ggctcacttc tgccagcagg tcttcaacgg
    28381 cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc tttataacgc cagaaaccga
    28441 ggccgttctt ctgaccaaag cggttggcat caaacagcgc gtcgatggca tcgcggtaat
    28501 ctttctgcat ccgctgcggg aagcctgctg ccatgacagc ctgagcgtga tgcgcggtat
    28561 caatgcccac aacgtccagc agatatgccg ggcccatcgg ccagccaaac tgtttttcca
    28621 tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag cagctggctg aaaccggcga
    28681 aatacgggaa cagcacgcgg ttaacaaaga agccggggca gtcgttaacc acaatcggcg
    28741 tcttgcccat cttgctcgcc caggcgacaa ctttcgcgat ggtttcgtcg gagcttttct
    28801 cgccgcgaat aatttctacc aacggcattc ggtggaccgg gttaaagaag tgcatcccgc
    28861 agaagttttc cgggcgttcc agcgcgttgg ccagttcgct gataggaatg gttgaagtgt
    28921 tagacgccag cacggtatcc tggcgtactt tttgttcggt ttctgccagt acggcttttt
    28981 tcactttcgg gttttcaaca accgcttcta ccacaatatc cacgcggtca aatccggcgt
    29041 agtcgagcgt tgggtggatc gtggagatca cgccagccag tttcagacca tcgattttgc
    29101 cgcgctcaag ctgcttgttc agcagtttcg cggcttcggt catgccgagg gttaacgact
    29161 tgtcgttgat atctttcatg acaaccggca cgcctttcca cgcggactgg taagcgatac
    29221 cgccacccat gatgcctgcg cccagcacag cagcctgttt cggggtttca acgtctttgg
    29281 tgagtttctt cgctttgcct tttacatatt gatcgttaag gaaaatgccg accagtgcgc
    29341 gagcttcgtt ggtatgcgcc agcgggacaa aacttttgtt ttccagattt aaggcttctt
    29401 cacgaccaaa acgggccgca gcttcaatgg tttttactgc ggtgatgggg gccggataat
    29461 gtttccccgc cgtttgcgcg accattcctt tagcgatggt gaagctcatg gtggcttcaa
    29521 tcttgctcag ttttagcggt tcctgcttcg gctgacgttt cgctttccag tcgaggtcgc
    29581 cgttaatggc ctggcgtaaa atcgccatcg ccccttcaat cagtttttct gctttgacta
    29641 cgccatccac cagaccgatt ttcagcgcct gatccgcgcc gacatcttta ccggcggcaa
    29701 tgatttccag tgcactgtca gcgcccagca tacgtggcat acgtacagaa ccgccaaagc
    29761 ccggcatgat gcccagtttg gtttccggca gaccgatgcg cagatccggc gtcgccagac
    29821 gataatcggt cgccagcacg cattcgcagc caccacccag cgcatagccg ttgacggcag
    29881 caatggtcgg caccggcaga tcttccaggc gattaaacac gctattggca aagtgcagcc
    29941 actgacttaa ctgttcttca ggaacgagga acagggacaa aaattcggtg atatcagcac
    30001 cgacgataaa ggctgctttg ttcgaacgca gcagcagccc ttttagatct gattgctgtt
    30061 ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc agtgtcgagt ttattaactg
    30121 aacctggggc atcaaatacc agttcggcaa tgccatcttc cagccagtca aggtacaggg
    30181 tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa ggtaatctgg tcgtaccaga
    30241 tgcatcggag tgtgtgtttt gtgttaaaaa tatgcaaata aaagattaaa gaaatgccga
    30301 tctgatcacg ctcggcagaa atcacgctct ggatgaacga tgtgctaaga tgcggagact
    30361 taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg gcctcgctct ataaaaatca
    30421 tatagctacc ttacaagaac ggactcgcga tgcgctggcg cgcttcaagc tggatgcatt
    30481 acttattcac tccggcgagc tgttcaacgt ttttctcgac gatcatccct atccgtttaa
    30541 agtgaacccg caattcaaag cgtgggtgcc ggtaactcag gtgccaaact gctggttgct
    30601 ggtggatggc gtgaacaagc cgaaactgtg gttctatctg ccggttgatt actggcacaa
    30661 cgtcgaaccg ctgccgacct ccttctggac tgaagatgtg gaagtgatcg cgctgccgaa
    30721 agccgatggc attggtagcc tgctgcctgc tgcgcgcggc aatatcggtt atatcggtcc
    30781 ggtgccggaa cgtgcgctgc aactgggtat tgaggccagc aacatcaacc cgaaaggggt
    30841 gatcgactac ctgcattatt accgctcctt caaaaccgag tacgaactgg cctgtatgcg
    30901 tgaagcgcag aaaatggcgg tcaatggtca tcgtgcggca gaagaagcgt tccgttctgg
    30961 catgagcgag ttcgatatca atattgccta tctgaccgcg accggtcatc gtgataccga
    31021 cgtaccttac agcaacattg tggcgcttaa cgaacacgct gcggtgttgc attacaccaa
    31081 actggaccac caggcaccgg aagagatgcg cagcttcctg ctggatgccg gggccgaata
    31141 taacggctat gcggctgacc tgacccgtac ctggtcggca aaaagcgaca acgactacgc
    31201 acagctggtg aaagacgtaa atgatgaaca actggcgctg atcgccacca tgaaagcagg
    31261 cgtcagctat gtggattacc acatccagtt ccatcagcgc atcgccaaac tgctgcgtaa
    31321 acatcaaatc atcaccgata tgagtgaaga agcgatggtc gaaaacgatc tcaccggacc
    31381 gtttatgccg cacggtattg gccatccgct gggcctgcag gtgcatgacg tcgccggttt
    31441 tatgcaggat gatagcggta cacacctcgc ggcaccggca aaatatccgt acctgcgcta
    31501 cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa ccgggtatct acttcattga
    31561 atcgcttctg gcaccgtggc gtgaagggca gttcagcaag cacttcaact ggcagaaaat
    31621 tgaagcactg aaaccgttcg gcggcattcg tatcgaagac aacgtggtga tccacgaaaa
    31681 caacgtggaa aacatgaccc gggatctgaa actggcgtga tggaaagctg gttaattcct
    31741 gcggcaccgg tcacggtcgt tgaagagatc aaaaagagcc gcttcataac gatgttggcg
    31801 cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat cggtgcgggc agaacacccc
    31861 gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc cggatgattc tcaacagctg
    31921 ggtttctctg acgacgggga gccggcggga acggcaggta aaccgatgct cgcccagcta
    31981 atgggcagcg gcgtcgggga aattaccgct gtggtagtgc gctactacgg cggcatattg
    32041 cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg tgaatcaggc gctgcgccag
    32101 ctaacgaccc aacgcaagac gccattaacc gaatatactt tgcaatgtga atatagtcag
    32161 ttaaccggca ttgaagcgtt gctggggcag tgtgacggca aaattatcaa cagtgattat
    32221 caggcattcg ttctgctgcg ggtggcgctt ccggcggcga aagtggctga attttccgca
    32281 aagctggcgg attttagccg tggttcattg caattgttag cgattgaaga ataatcccca
    32341 cttcgttttg cagaactaag gaagcggcag agatgcattt tcgcgccatt acccgaatcg
    32401 ttggactact ggtcatctta ttttcaggga ccatgattat ccctgggctg gtagcactca
    32461 tctaccggga tggagcgggc cgcgctttta cccagacctt ttttgtcgcc ctcgccattg
    32521 gctctatgct gtggtggccg aaccgcaaag agaagggcga actgaaatcc cgtgaggggt
    32581 ttctgatagt ggtgctgttt tggaccgtgc tgggtagtgt cggtgcgctc ccttttatct
    32641 tctcggaaag cccgaacctc acgattaccg atgcgttttt tgaatctttc tctggcctga
    32701 ccaccaccgg ggccactacg ctggtggggc tggattcgct ccctcatgcc atcctctttt
    32761 atcgccagat gctgcaatgg tttggcggga tggggatcat cgtgttagcg gttgcgatac
    32821 tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc agaaatgccc ggcccgctga
    32881 aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa aactctgtgg ttgatttatg
    32941 tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc cggaatggat gcctttgatg
    33001 ccatcggcca tagctttgcg actatcgcta ttggcggctt ctcgacacat gatgccagta
    33061 tcggttattt cgacagtccg actattaaca ctatcattgc tatcttcctg ctgatctccg
    33121 gctgtaacta cggtctgcac ttttcactgt taagtgggcg tagtctgaag gtttattggc
    33181 gcgatccgga atttcgcatg tttatcggcg tacagtttac gctggtggtt atttgtacac
    33241 tcgtactgtg gtttcataat gtctacagtt cggcgctgat gacaattaac caggcgtttt
    33301 tccaggtggt atcgatggcg acaaccgccg ggtttacgac tgacagcatt gcccgctggc
    33361 cgctcttttt gccggtactg cttttatgtt cagcttttat cggcggttgc gccgggtcaa
    33421 cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt taagcagggg aaccgtgagc
    33481 tgaaacgact ggtgcatccg aacgccgtct atagcattaa gctggggaat cgcgcactgc
    33541 cggaacgtat cctcgaagcc gtttggggat ttttctccgc ctatgcattg gtgtttattg
    33601 tcagtatgct ggcgattatc gccacgggcg tggatgattt ttctgccttt gcctcagttg
    33661 ttgcgacatt gaataacctg gggccagggc ttggcgtggt tgctgataac tttaccagta
    33721 tgaacccggt ggctaaatgg atcctgattg ccaacatgct gtttggtcgt ctcgaggtct
    33781 ttacattact ggtgctcttt accccgactt tctggcgtga atgatggagt aatacgtgaa
    33841 aacattaatt cttttctcaa caagggacgg acaaacgcgc gagattgcct cctacctggc
    33901 ttcggaactg aaagaactgg ggatccagac ggatgtcgcc aatgtgcacc gcattgaaga
    33961 accacaatgg gaaaactatg accgtgtggt cattggtgct tctattcgct atggtcacta
    34021 ccactctgcg ttccaggaat ttgtcaaaaa acatgcgacg cgactgaatt cgatgccgag
    34081 cgccttttat tccgtaaacc tggtggcgcg caaaccggag aagcgtactc cacagaccaa
    34141 cagctacgcg cggaagtttc tgatgaactc gcaatggcgt cccgatcgct gcgcggtcat
    34201 tgccggggcg ctgcgttacc cacgttatcg ctggtacgac cgttttatgc tcaagctgat
    34261 tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa gttgtctata ccgattggga
    34321 gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc gacaaaccga cgctgaaata
    34381 agcataaaga ataaaaaatg cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc
    34441 ggaataactc cctataatgc gccaccactg acacggaaca acggcaaaca cgcagccggg
    34501 tcagcggggt tctcctgaga attccgacag agaaagcaaa aataaatg
//
LOCUS       DAJTRN010000036        34067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRN010000036 DAJTRN010000000
VERSION     DAJTRN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..436)
                     /locus_tag="OO328_001780"
     CDS             complement(<1..436)
                     /locus_tag="OO328_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5930369.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVP"
     gene            1052..1189
                     /locus_tag="OO328_001781"
     CDS             1052..1189
                     /locus_tag="OO328_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930370.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1789..2568
                     /gene="ipaJ"
                     /locus_tag="OO328_001782"
     CDS             1789..2568
                     /gene="ipaJ"
                     /locus_tag="OO328_001782"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5930371.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3515..4444)
                     /gene="virB"
                     /locus_tag="OO328_001783"
     CDS             complement(3515..4444)
                     /gene="virB"
                     /locus_tag="OO328_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5930372.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4661..4897)
                     /locus_tag="OO328_001784"
     CDS             complement(4661..4897)
                     /locus_tag="OO328_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5930373.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4901..6802)
                     /gene="ipaA"
                     /locus_tag="OO328_001785"
     CDS             complement(4901..6802)
                     /gene="ipaA"
                     /locus_tag="OO328_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5930374.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6811..7809)
                     /gene="ipaD"
                     /locus_tag="OO328_001786"
     CDS             complement(6811..7809)
                     /gene="ipaD"
                     /locus_tag="OO328_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5930375.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7860..8951)
                     /gene="ipaC"
                     /locus_tag="OO328_001787"
     CDS             complement(7860..8951)
                     /gene="ipaC"
                     /locus_tag="OO328_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5930376.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8971..10713)
                     /gene="ipaB"
                     /locus_tag="OO328_001788"
     CDS             complement(8971..10713)
                     /gene="ipaB"
                     /locus_tag="OO328_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5930377.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10719..11186)
                     /gene="ipgC"
                     /locus_tag="OO328_001789"
     CDS             complement(10719..11186)
                     /gene="ipgC"
                     /locus_tag="OO328_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5930378.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11243..11869)
                     /gene="ipgB1"
                     /locus_tag="OO328_001790"
     CDS             complement(11243..11869)
                     /gene="ipgB1"
                     /locus_tag="OO328_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5930379.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11885..12274)
                     /gene="ipgA"
                     /locus_tag="OO328_001791"
     CDS             complement(11885..12274)
                     /gene="ipgA"
                     /locus_tag="OO328_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5930380.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12287..13771)
                     /gene="icsB"
                     /locus_tag="OO328_001792"
     CDS             complement(12287..13771)
                     /gene="icsB"
                     /locus_tag="OO328_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5930381.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            14085..15701
                     /locus_tag="OO328_001793"
     CDS             14085..15701
                     /locus_tag="OO328_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5930382.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15711..16073
                     /gene="ipgE"
                     /locus_tag="OO328_001794"
     CDS             15711..16073
                     /gene="ipgE"
                     /locus_tag="OO328_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5930383.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            16073..16531
                     /gene="ipgF"
                     /locus_tag="OO328_001795"
     CDS             16073..16531
                     /gene="ipgF"
                     /locus_tag="OO328_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5930384.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16540..17655
                     /gene="mxiG"
                     /locus_tag="OO328_001796"
     CDS             16540..17655
                     /gene="mxiG"
                     /locus_tag="OO328_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5930385.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17663..17914
                     /gene="mxiH"
                     /locus_tag="OO328_001797"
     CDS             17663..17914
                     /gene="mxiH"
                     /locus_tag="OO328_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5930386.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17927..18220
                     /gene="mxiI"
                     /locus_tag="OO328_001798"
     CDS             17927..18220
                     /gene="mxiI"
                     /locus_tag="OO328_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5930387.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18226..18951
                     /gene="mxiJ"
                     /locus_tag="OO328_001799"
     CDS             18226..18951
                     /gene="mxiJ"
                     /locus_tag="OO328_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5930388.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18948..19475
                     /locus_tag="OO328_001800"
     CDS             18948..19475
                     /locus_tag="OO328_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5930389.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19417..20112
                     /gene="mxiN"
                     /locus_tag="OO328_001801"
     CDS             19417..20112
                     /gene="mxiN"
                     /locus_tag="OO328_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5930390.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20105..20512
                     /gene="mxiM"
                     /locus_tag="OO328_001802"
     CDS             20105..20512
                     /gene="mxiM"
                     /locus_tag="OO328_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5930391.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20496..20924
                     /gene="mxiM"
                     /locus_tag="OO328_001803"
     CDS             20496..20924
                     /gene="mxiM"
                     /locus_tag="OO328_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5930392.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            21054..21686
                     /gene="mxiE"
                     /locus_tag="OO328_001804"
     CDS             21054..21686
                     /gene="mxiE"
                     /locus_tag="OO328_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5930393.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21673..23373
                     /gene="mxiD"
                     /locus_tag="OO328_001805"
     CDS             21673..23373
                     /gene="mxiD"
                     /locus_tag="OO328_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5930394.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23392..24459
                     /gene="mxiC"
                     /locus_tag="OO328_001806"
     CDS             23392..24459
                     /gene="mxiC"
                     /locus_tag="OO328_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5930395.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24472..26532
                     /gene="mxiA"
                     /locus_tag="OO328_001807"
                     /gene_synonym="virH"
     CDS             24472..26532
                     /gene="mxiA"
                     /locus_tag="OO328_001807"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5930396.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26545..26946
                     /gene="spa15"
                     /locus_tag="OO328_001808"
                     /gene_synonym="spaK"
     CDS             26545..26946
                     /gene="spa15"
                     /locus_tag="OO328_001808"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5930397.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26950..28242
                     /gene="spaL"
                     /locus_tag="OO328_001809"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26950..28242
                     /gene="spaL"
                     /locus_tag="OO328_001809"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5930398.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28335..28673
                     /gene="spa13"
                     /locus_tag="OO328_001810"
                     /gene_synonym="spaM"
     CDS             28335..28673
                     /gene="spa13"
                     /locus_tag="OO328_001810"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5930399.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28660..29538
                     /gene="spa32"
                     /locus_tag="OO328_001811"
     CDS             28660..29538
                     /gene="spa32"
                     /locus_tag="OO328_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5930400.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29532..30413
                     /gene="spa33"
                     /locus_tag="OO328_001812"
                     /gene_synonym="spaO"
     CDS             29532..30413
                     /gene="spa33"
                     /locus_tag="OO328_001812"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5930401.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30418..31068
                     /gene="spa24"
                     /locus_tag="OO328_001813"
                     /gene_synonym="spaP"
     CDS             30418..31068
                     /gene="spa24"
                     /locus_tag="OO328_001813"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5930402.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            31083..31343
                     /gene="spaQ"
                     /locus_tag="OO328_001814"
     CDS             31083..31343
                     /gene="spaQ"
                     /locus_tag="OO328_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5930403.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31343..32113
                     /gene="spa29"
                     /locus_tag="OO328_001815"
                     /gene_synonym="spaR"
     CDS             31343..32113
                     /gene="spa29"
                     /locus_tag="OO328_001815"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5930404.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32120..33148
                     /gene="spa40"
                     /locus_tag="OO328_001816"
                     /gene_synonym="spaS"
     CDS             32120..33148
                     /gene="spa40"
                     /locus_tag="OO328_001816"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5930405.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33161..33403
                     /locus_tag="OO328_001817"
     CDS             33161..33403
                     /locus_tag="OO328_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930406.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10591 a         5005 c         6854 g        11617 t
ORIGIN      
        1 cgggaacagc aacgaacacg tgaagcggtg agcgaccatt acgcatagtg ccccagtcaa
       61 cctgcatctg tcgtccgggt tcagtttcga accgaacggc aggctcctgc tcctgaggaa
      121 ccgagagaga acgaatgaat gccctgagaa tggtcattcc gccacgatat ccctggtctc
      181 tgatctcacg agcgattacc gttgccggga ttttgtaagg atgagcatcg gcgatgcgtt
      241 gacgaatata atcccggtat tcatccagga gtgaagcaac agcaggtcgc ggcgtatatt
      301 ttggcggctc agattttgcc tgcaaataac gtttaacggt attgcgggag agccccagtt
      361 ctctggcaat cgtccggcta ctcattccct gcttgtgcag gattttaatt tccataactg
      421 tctcaaaagt gaccataagc tctcctgaat caggagagca gattaccccc tggatctgat
      481 ttcaggcgtt gggtgtggat cactattgca ccgttcgtga cacttgttga ctacctgtgc
      541 ggcatctggt atcaaggtgg cgtgaagccg gttcaggttc tcatgcggaa cgttggaacc
      601 tatatccccc cgataccaaa ggcgaaatgt aagtgacaaa atgcaatgaa cacgttctcc
      661 gttaactgag gagcatggtg ataaataaag gtaaatgcag aaggtgtaga gtctcgcaga
      721 tttctgacat catagagcct aacattagat gcataacgct acagattgta gaatctctac
      781 aggtgaaata tacactggtt ggcgtttatg tcgcaaaaca tgtcattcag gttcatctca
      841 ccaataagga tatgagtgaa gtggaggata agtgagtctg ctgtcagagt tttctggtgt
      901 atgtcagtaa ctgcgaacca tgtctgcttg atatgggagc ttatggaggc tctctgtaca
      961 aggttaggga gctaacaaag cagggataaa aagtccgcct gatgcaggca cggttcgtta
     1021 aatcttttgc cattggtaac aaaatggtgc gatgaatgtt caggctatct ttatcttgat
     1081 ggtggttcag ctgtttggta aagaaatcgc tgtaacaata gaagaactgc aggcagtact
     1141 ggtcccacat cgtttgctca gtcaactggt gaagttccgt agcgcgtaaa ttaatgcttt
     1201 gcatgaaacg ttactggagt ggatgaaaga atacctgcat atatcattac tgcgttggat
     1261 taccagtaca acagattgaa tgaattggac atgttgatag acggcattga aaagcagctt
     1321 ataagcatag tgcggcagag ttaattggag agcacttcgc cttctgagta ttaagtaagc
     1381 atggtggtac ctgcggatac tatctattca ctgtgctcgg tcggcagcct tactgaccaa
     1441 ggagttgggg gagttgataa caaagttgaa aaaacagctc tcaaccaagg tggttattgt
     1501 aacaatagca aataagctgg tgtgacctgt atcacaataa ccgattatgg acgtaaatac
     1561 gacaaaaata tgtcagtatc aggcggtatt aattgctgat gctgatggac attaactctt
     1621 aataaaaggg tggatgctga aaggttgtta tggtgacgga cgtgatgacg acggaaaaga
     1681 gtatgaggtt ttggatttaa agttcgttcg gggaataagg acccggtcag ctaattctca
     1741 tagtagttcg cattgctgga tgcaatactg tgctgcaatt actcgtcgat gtcggaacaa
     1801 cggaagcctt gcaaacgggg atgtattcat acaggtgtta tgttatatgg ggttttattg
     1861 cagggggcta taccaagaga atatatgata tctcatcaga ctgatgtgag ggtaaatgag
     1921 aatagagtaa acgaacaagg ttgttttttg gcgaggaagc agatgtatga caattcttgt
     1981 ggagctgcca gtttactctg tgctgcaaag gaattagggg ttgataaaat accacaatac
     2041 aaagggtcta tgtccgaaat gacaaggaag agttctcttg atttggataa tcgttgtgag
     2101 cgggatttat acttaataac tagtggtaat tataatccga gaatacacaa agataatatc
     2161 gcggatgccg gttattctat gcctgataag atagtcatgg ctacgaggct tcttggattg
     2221 aatgcttatg tcgttgaaga aagtaacatt ttttcgcaag taattagttt tatttatccc
     2281 gatgcaaggg acttattaat tggcatggga tgtaatatag tacatcaacg agatgtgtta
     2341 tcttctaacc aaagagtttt ggaagctgtt gctgtatcct ttattggagt tcctgtcggc
     2401 ctccactggg tgttgtgtag acctgatgga agctatatgg atccagctgt aggagaaaat
     2461 tattcttgtt tttcaactat ggaattaggt gcaagaagaa gtaattctaa ctttatagga
     2521 tatactaaaa taggtatttc catagttata actaatgagg ctttgtaaat tgtcgttttt
     2581 tatctgtgaa ggagcttgaa tattttgggg tggggggcat aacaaatgat attactgagt
     2641 acaaatttag ctatattggg cggattagtt gtgtgggaag acgaaatgtt tctcttgaaa
     2701 taagtgaact ccctcaagga atgattgtgt gcttaaatat ttaagtgctg gtaagtttga
     2761 gaaggctgac atattttgca gagcaaacag tgattgactt ggatgatatt tttattttag
     2821 tcactcgttg taattggagt atgctttatt ttatttgaaa atagatgtat tttatacccg
     2881 caatttaaac tgtaagccct atgaggggta tggatattat aagttactct ttgaccaagc
     2941 ataacttttg gggttagaaa aataaatctc ttgttaataa ttaatatgat tttaaaatca
     3001 aacttgttta taattataaa ataatgtaag tcggtcattt tatgggaaga ttaggagaat
     3061 aaaacagtaa ttatatttga gaaatctgct aatatattct taaagctttt ttgatactgt
     3121 gaacgaagtc tgttaatggt ggattttttg tttatcacca tgtaaaaaaa attgagtagt
     3181 aaaagcgttg actgctaatc ctcgctgttt ttattgaaac gattaaaaat acgctgaata
     3241 ttttcggcca gtcactcata ttcacataaa attttgacca tcatgccgca tcccttagtt
     3301 gcacaattca ttttcaatga tatgaatatt actaatttca ccagataatt ttctcgtggt
     3361 cagcagggta tgagttttca ccgtaatttt aaggaaacac agtgcgtggg gattgaataa
     3421 cagatagccg acggaattac cccaccggct gaaagtcaag ttcaacgaag atttttatta
     3481 tctgaattgg gcagtttaca tcagtgttcg atgtttatga agacgataga tggcgagaaa
     3541 ttatatcccg aatagcttca tctatttctc tctgaactgt tttgtttatt ttagataatg
     3601 taaatattag agttctgttt gttttattct cttttctttt tatgtaagtc cgtttgtctt
     3661 tagaaataaa taacgagtca acttttagcg aagggttttg ctttctgttt ttgcttttct
     3721 ttatgatgtt taaaatttct tttttatata tgtccggggg caaatttgta tcaaggtctt
     3781 ttatttcttc ctttaatatt ggtagtgtag aactaagaga ttcattagcc ttttcaagtc
     3841 ctttataata attgaataat atcttgtagt cattaaagtt aagttctgac gcgattggaa
     3901 atagagatat tatttcctgt ggaacgcttg ctgcctgaaa ggcacgagtg actttcgcgc
     3961 gagacagatt ctcttttttg gctatgtctt tataggacat ccctgatact ttcagaaaat
     4021 taagaccaat accaagttct cggatgctat gctcttttgc tgtttgtata tcgtttgcta
     4081 gttttctggc atcaagagta gatatatatt cttttgaata tagaacttca agatctgctc
     4141 ctgcatatat tgcagatgct cttctacgag tgccatccag aatttcaatt ctaccatcaa
     4201 tctcccttcc tattacaggg aagaattgtt gtagctttat agtttttatg atatcggcta
     4261 atgattcttc tgttagggat tcttgatcgc gtacattcgt tttttggttg acgaaggtta
     4321 aatcttggat ccttttatga ggaatcttgg ctttgataaa ggaaacttta ttaccagaat
     4381 ggagtgtaaa ctctttcttt tggccttcct ttatacttaa caagtcgttg cacaaatcca
     4441 ccatatcaca ccctgtttat tcatattgat ttatattgac aaaagttaaa tgcagtgtga
     4501 gcaagattgt atgtaactgt tgatgatagt caacggtagt cttaaaaatt actattttaa
     4561 tagctggatt gatgcaattt atctttattg gataaaagaa taatattctt gtggagctct
     4621 gatgtgagag ctccatagat gaaaaaatgc ttatcaatta ctatattgat tttgtactat
     4681 ttttgactac agaaataaga tctccaatag taataatgtg ttctaatgta gattcatcta
     4741 tacgtagatt gaactcaaaa ctaatcatat ccagcatttc aatcagagaa agagaatcgg
     4801 cagaaagatc ttcaataaat ttagtttctt cagatagttt gctgtacgct ataccatagc
     4861 aaaaggccac tattgataat attttttctt ttatcatatt ttaatcctta ttgatattct
     4921 ttaatacttt tgatagggaa gtggttacat cttttgctgc tttatatata ttattgtttt
     4981 cagctgtaat atcattgtta ttttttaaat cagatatatc atctgtaagt aattctgcag
     5041 aggtatcgtc aatttttgac aataccttgc tgagggcgct agatacttct tttgcctttt
     5101 catagatcgc atgattttta tcaattgtat ctgcgttggt ttctgttacg tagtttctta
     5161 tatttgcatc tgtaactttt aatttgttgt ttattctatg ttctgttgtg gattttgtaa
     5221 tgtcctttga tttttcctgt aactctcgtt cttgtgttcc tgatttagtt ccaatgagag
     5281 atataagatt acttagggag tttgttactt tttttgaagc ttcaaatatc gtttcccgtg
     5341 ttaaatatgt gtctccattt cttgataaaa aatcagtctg aagtactttt gaatcagtgt
     5401 ttgagttgaa tttattgatt tgcgaagcta ctctatgaga aagagaagcg ctacttttag
     5461 ttgtatctga tgagttatca ttactgcttg tcactccttc cgggtggaga gggggggtgg
     5521 ttgaagagtt ctgtatgttc agaggcctgt gtccccgaga aagagagtta tttgcagatc
     5581 tttcttgact actcgtagtt aacaggtctg aacctattcc atccgaatta tctggagtat
     5641 ggttgaaagc cttttctaaa gctgaattat ttgcaaatgt ctggacagta tctgatatag
     5701 agtgtggtag aacggaagtt actttttgaa ctagtgctct ttgtggattt agtaatgagt
     5761 ttccgttacg ggataataga tcgtttgtct gagaagtagg agactgtgca tcattttctg
     5821 ggttctcgtg atagctattg tcatatactc tgttgtcaaa aactctatta tcgtaagttc
     5881 tattatcatt atttatatga tagtgaatta caggttggga tgttttctca ttggctccgg
     5941 gggttggtcc gccatctggc atatcttcgg gaggtgtagg gaggccatct tggataggat
     6001 gcgaatcttt tatatcttct aaaatatcat taaggacttt tctcaacagc tcaggaaaca
     6061 cggctactgt atttaagtct cttgacagtt tacctatacc aaatccttta ctatcaatag
     6121 tatttttacg ttgattgcgc aaactatcaa aggcaagttt tgcctgtgcg tcaactaaat
     6181 catctagttt gagagatgaa acagtttcat tacttaactt taattcagca agaatattat
     6241 tcttgataaa tgatgatact tcagtattga ggagtttttg tcccaatgat aaagcttcac
     6301 cagatgtgtg ttgttgtgct aatagtgcaa gtctgtgctc aatgttttgt cgtaattgat
     6361 ggagtttctt attctttgcc gaccatggtc caaaggcaga atcttgggat aatttatctt
     6421 ttataattag ttccgaatac tgtgcgacaa ttttttgaag tcccttttcg ttttcaaaat
     6481 taagaccatt ttgatgtact aaggagtaca aaacatttga aaattcgaga atctcgcttt
     6541 tggcatacgc attcattgag ttggaataaa tgcttcttaa cacatcttct ttccctgagg
     6601 aagaaaataa aaaatcaaga catttctgat taaaagacgt tgcaaagttt tctttttcag
     6661 acactgatgc tggtgttttt agagaagttt gcgaactatg gatatcggat attttgcttt
     6721 ttaactcgct gtattctgtt gatgatggtg aagttgcctt atataagaat gttggcgctt
     6781 gagtattatt tacattatgc atggcgcacc tcagaaatgg agaaaaagtt tatctgtatc
     6841 tgtacatgag cttattgtac tactcaaaac ctttactaaa ttatcaaaaa tactattggc
     6901 attactgtat ttttgaacta aagtttgaag attatttttc attgtttcat cttcggcaga
     6961 gaatccggca ttccatgcct gatattttgc attatctagc acaacctcgc catttccacc
     7021 tagattatct aagcttttta acatattgtc tattggggtc atgtttatac tgacaacata
     7081 tcccccgttt ttttgagata ccttgccgat tgttccacct aattctgtaa gccatttatt
     7141 tgcttgttcc tgactaacag tattatttgc tggatatagc ggtttatctt tatatttttc
     7201 cttgagttct tccaatgcct ttttaagcga gttgacttgt aatttcacgg agtttccgtc
     7261 gttacctccg ggagagatcc agccggcaag actggaaaga acagcgctaa aatcttgata
     7321 catttgagta tatgaactaa cggcatgttc atatactttc agatactgtt cattaatatc
     7381 attgatggag tttgcaattt tagcccacag ttctctatga gatatcattt gatctccatc
     7441 aagctcggct tctttcggag ctgaatgtaa taattctctt gcgtctttat taattggata
     7501 ttcgtgcctg gaaagaatat ctaatagttg agcggattta tttacatcca tgctaatctg
     7561 agatgaatgt aatgctattt cttctagcga tgttttagtc aacgtttttt gtgaaagctc
     7621 tttctttaat gcctgatttg ttgttctgat attatgaagg gtgtcgttga gcatagtaag
     7681 ggaacttaca ggatgagaac tggtcgttgt ttttatatca gaattaactg tttcggttga
     7741 tgaaccgttg gtattgtttg gactgaatga tgaggtggaa atactattag tcagagttgt
     7801 tatattcatg gttatttcct tacaggaaga gccatatatt attatggctc ttggatatat
     7861 taagctcgaa tgttaccagc aatctgactg gctgtcgaat tctttgattg gttgatgctg
     7921 tcaattatat tcaataattt ttgtattaat tgatttgtcg cttgggatgc ttctttagat
     7981 acttgggatg cttcctctgc ttgtttactg ctggcctgac tgattagttg ttcttcttct
     8041 tcaagagcag atgcataacg ccctcctgat gtggatatat taccagcaac ggctgatgtt
     8101 tccattgttg cacggccaat gttttgtttt tgctgcgcag aaagggtatt gagctcgtaa
     8161 gttcttctct gggtgtcaat tttatcctgc aaagaaatat cgggagaact aagagaagtt
     8221 ttatgttcag ttgacaggga tatattatct ggcgccagtt tatttttacc taaaaatttt
     8281 gtgctagagt tagtttgtgg tgaggtgata tttgtatcta tttgtttatt taaccctaat
     8341 ttagaacctg caagctcttt ttcaagagat tgagcagtgg caaggttctt tcttaaggct
     8401 cctttttggt cgctaatccc tgaatgcgtt tttttggcac cgatacccgt tatacctact
     8461 tgtgtgactg ctccagtaat gcttgatgat agggctgcca ggccttgccg aacaatgttc
     8521 tctgcagctg attttgtagc atcgaacgca atcaatgaca attgagagcc caatttagtt
     8581 tctgcagtgc ggagagcaga aagtagaacg gctacactaa taattaaaga aacagcatta
     8641 gaagaaagac tggaaatatc cagagtgttc tctggagtaa gggtgtgact catctctgaa
     8701 agatctttca tctttttgtc agtaaggcga gcaatgattt ctgaatcatg ctgaacctgc
     8761 tctgataact ttaaaagttg atcatcattt aatgtggttg ttaatacggg atttttaccg
     8821 acattaagtg gaatatgcgc tgcaatctgc tgataatttt gtgatttttg tgtttcggaa
     8881 gaactttgag tttgttttgt ggatatatct gtatataaaa tctgggttgg ttttgtgttt
     8941 tgaatttcca taacattctc cttatttgta tcaagcagta gtttgttgca aaattgcttt
     9001 tgcaacatca gttctattag cctgagagtt ggacattgag gctaatagat ctgcaattac
     9061 ttcctgcaat tggccgaatt tttctattgc ttcactgata tattttgaca gttgttcaac
     9121 ttgatatttc gataatgtca ggtctgctag atttgtcgac gcgctgttct ggaaaacagc
     9181 agaagcgaca cttcctcccg cttgtgtggc agagttaaca ctttctccta ataaaactgc
     9241 ttggtttaaa tgggtggaaa taatttgttt ggatattact tcatcacccg ctgcaccaag
     9301 aaatttattt aatctggcaa cagcattagt gattaaatcg tccagttgag aagaaaaatt
     9361 cttgagaaac tttggtataa ggtctgtgag ggttttacct attattttgc caatattttc
     9421 tgcaagtttt gctgctgcct gtttaccaac agtggctacg agaacgactg ctgcaactag
     9481 gacaagagcg cctgcgattg cccccagaat agagccaatc attttggctt ttttcgagtc
     9541 gacgcccaag ccttcgagca tttttgtaaa tgcatctgaa aggagtttga ttaagggttc
     9601 aatgactgct ttcatgatcg gattcagggc ttgttccatg aaggaattgc cggtcgctgc
     9661 ttgtactata gcatccgtaa ccataagagc taaaccaaca gctgccagtg ctagagaggc
     9721 tcctccagaa aaagctgctg caacaacact aacgatagtt aaaagtgccc caagtatttt
     9781 cccaacacaa cccattactc tgttgagttc ttctgcttta cgtacttcag cagcatactc
     9841 atcagatttt ctctccattt cagtttttct tgattcttgg agagactgga atagagccag
     9901 atcatttttt aaagattctt cattattttt tccaactagt tgaataaagg ttgccatcaa
     9961 ttgagtaaca ctggcaagtc cggttaatga tttctgctgg gttgatagct gttcagcaga
    10021 tgctgtgttt gaaaatgcag aaaaagagtc tatttctttt tcgagttgca ttgatttatc
    10081 tgtaagtttg ctatgaattg acagggtttt ctgctcaatc aatgtcctgt ctttaactgc
    10141 tgcgtctttt ttgatagtga gttgtatttc ttcccggctt aattttttct tttctggtga
    10201 ctctgggtcg agttcggata atcttgtttg aatttggtta attttatttt ctaggtcttt
    10261 tattttagaa tctgcgtttt ttagtttatt aatttgtttt tcatagtctc tggttagtcc
    10321 ttcagtttca gatagaagag tgttaatttt atcggagaat tctaggtttt tttgctgtct
    10381 tgcctgttgc tgggacttcc aagcagtaat tttatttgtt aatgcagtta aagatttttc
    10441 accgagtatt tgaataaggt ttccaattaa aagcgttagt tgggaacttg catttaatga
    10501 ctttggtgct ttaagttcag ggattaatat atttgaagtt gagtgtgcat tagttgtatt
    10561 aatattttgg ttagcagtaa gatcagcaat tgtaagagaa aataatttgt tagctgcatc
    10621 atttgcagct tggatagtat tgtctccaag ctcagtggaa gccaatattt tggcaagagg
    10681 aaaaccagtg gttgtggtgc ttacattatg cataataatt actccttgat atcctgaatt
    10741 gcgtccaagt atgattgtgc ttttattttt aatttttcat cattgctgtg ttgaattacg
    10801 agttcgaagc actctttagc ttttaagggg gctttcaacc gaagctggca ttgtccagta
    10861 tggaatactg gtgtatagtc attttttcct aatgcaaaag cgacagcata aaggtctgct
    10921 gcttgttgga actgttcttt tatctgataa atagctgcga gtcccataat gtagtctaca
    10981 ttgtaaaagt cgtatataca taaaaacctg aagaaaactt cagcttcctc tattcttcct
    11041 ttgttgtaaa agtcataagc atatgaataa atgtcatcca tcatatcatc aggaattgca
    11101 ttaatatctt tcagtgtagc gccagagtta attgcatcaa ttactgcagt agagatgctt
    11161 tcattttcgg tgatatttaa agacataagg tctccttttt gtctgcttta ctttaataag
    11221 tataagattt aatataaaag atttaatttg tattgctttg acggtataca gcctttagaa
    11281 cctctgcgct tatttctatt tgtaaaactt ttgttgattc attaaatatt ttatcgtaga
    11341 cggaactgga tactagattt gtaagatcct tcatttgtgt gttaaacaaa tccatgtcac
    11401 tgctatagcg ttttaaaatc tttgctctga ggccagatga ggcaatctcg tttagtgctg
    11461 tatgactgat agatgtttgt gctgattttt catcaagttt tatatccggg aatttttttt
    11521 caatagctgc aaatatgttt tttttgttat ctgaggtcaa aacaactcca ttgtgtatgg
    11581 ccatttcatc tatacttctg tttatcattg aagagacata agtggttcgc tcttggctca
    11641 tccaacaaaa tagttgattc cccgaattac tatcttttat tttgtctatg acgctgtccc
    11701 tgattttatt tatattcacc gggcatccaa aattcttctg aggaaagcgt tgttttaagt
    11761 taaaaacaga taatattttc tttacaagct tattgcgact taacgaatca aatgatgggc
    11821 gaggaatcgc gaattcaacc tgtggaagta ttttgtttag aatttgcatg atacccccta
    11881 tatgttagtt cacttctgaa gtgatgtttg caatggttag tttaatacaa aataaaaatt
    11941 catacaaaga ttcaatgagt tcatcaggtg aaagatattt tttgtagtgt ttcttggtca
    12001 aaacacattg atgagtatca gggagaagac agatagaaga accataatct ctactcaaag
    12061 agtagttaag gatgagtatg tgttctggat taataatatt ttctgtaaaa agttcatgtt
    12121 cattaagaag tccggtcaaa aagatgtatt catcattatc tgttatacaa acaggtattt
    12181 gttcttcgag caatataaag gcttgatttt tatcgttaaa atcaagcttt gcacctgtta
    12241 tttcataaag tttatcatat agtttgcgac acatttctat ggccttctat atattagaat
    12301 gagagttatt caataaaagc ttattgatct gtaataagtc gtgaatgtaa agtttggttc
    12361 caagatctgg cgatttaaga gaattgtaat aatcaataat ttttattgat ttttcgatga
    12421 tttcttcttt tttatctgtt gcttcggttt ttaattcgtt taatacacga gtcatattgc
    12481 tgggtgctga ttttactaag tatgagtggc aagcgttgat gaccgcatca aagtcttctg
    12541 aagatacatt ttcagtagga tgtttgtaaa gtgaaattga taaaggcttg aatgtttttg
    12601 gtgtgtggat aaggttaatt ttattgaaac ttttaagata gctgcgtcgt aattgagtca
    12661 atctggaaag atcaaaatca gattcaatat acttcattat ttcgttataa gactgattga
    12721 gactttcaat gcgttgccta actttatttg catacgcgta tacatcgtta ggtgtagcaa
    12781 ccaatttaac gtcagggagt ggaaagtata tttcagaatt tcctgcttta agaacattaa
    12841 gtgccatacc agcacagttt tcagatttac tgattaattt ataatttgcc aattgtctga
    12901 aagcatctgc tttctttttg catataaatt ttgacatgtt tgtttcatca aggccaaaaa
    12961 ggttatggct gattttacca tcctttgttt tattatcacc tgataatgga atgtatactt
    13021 tatcagcact gaccccccaa taaccatagg ggtctttttt ttgattagct ctgggaaaaa
    13081 atattttttt gttgttattt tgatcttgtt ttgaatttaa taagtttcta acctcttggc
    13141 ctgcatcaag tctttcggct gtttggtctg aaatcatatt tagtttgtct tgcttatagg
    13201 tatcgacact gtaacctgag cgtttttcaa aaaggtagtt tatttctgta tcttttgtaa
    13261 cattcccgcc gggataccag ctgagatagg tatgagttgg ttcctgtagt aaattgtttt
    13321 ttatactgat actagtatgc cctaaatggg ttatgcctct atttccccag caacgaaaaa
    13381 ctaaatgtct ttcaagattt ttggctgtac cgatgccatg aaaacgctct gcggtattca
    13441 acattaattt gatgtctcta cctgtgaaag agtgttgaat tgagttttta gctgcttcat
    13501 aggcggcaaa cgcactttct gcagattttt cgctagctaa tgtattgagg aaaagcaata
    13561 aggatttttg attctctagt cgataatttt ttagttgttc atttaatatc tgtgaattta
    13621 tctttgcatt aatagtgctt aatgttctga aaaagagatc ctttaggttg aatttctgtg
    13681 ctatcaatat aggtccatgc tctgtatctt caacgcgtat aggccctttt gtattgcttg
    13741 cgtcaatgaa attgctaatt ttgaggatca tactttatta actctccatt acttggtgat
    13801 tgtggggatg taaaatgtat ttggtatata atatattcaa ttaataaatt tagaaacttg
    13861 agcctgttaa cataatcaaa ttttcttttg ctgtacataa tatgtacctc gtgagcatat
    13921 gtagtgctcg tttcatcatg aaatcccaca agataaagtg cctgatgtat caggctcgga
    13981 gtgttataga aaaagagaga accctgttga ataagtatct tttttgttgt aaaacgaggt
    14041 acaaaactgc aatcaatgcc ccatttatga ctgaggataa ttaaatgcac ataactaatt
    14101 tgggattgca tcaggtttca tttcaaagcg gagattccta taaaggcgca gaagaaaccg
    14161 gaaagcacaa aggtgtaagc gtgatttcat atcaaagagt taaaaatgga gaaagaaata
    14221 aggggatcga agcgcttaat cgattatatt tacagaacca aacaagctta acaggaaaaa
    14281 gccttttgtt tgcgcgtgac agagctgaag ttttttgcga ggcaataaag ttggcaggtg
    14341 gtgatacgtc aaaaattaaa gccatgatgg aacgattaga tacctataaa cttggtgaag
    14401 ttaataaaag acatattaat gagcttaata aagtaataag tgaagagata agagcacagc
    14461 taggcattaa aaataaaaag gaattgcaaa cgaaaataaa acaaatattt actgattatt
    14521 taaataataa gaattgggga ccggtcgaca agaatattag tcatcatggg aaaaattatg
    14581 gttttcaatt aactcctgcc tctcatatga agatagggaa taaaaatata tttgtcaaag
    14641 agtataatgg aaaaggaatt tgttgtgctt ctacgagaga gagtgatcat atcgcgaata
    14701 tgtggctttc aaaagtggtg gatgatgagg ggaaggaaat tttttcgggt atcagacatg
    14761 gagttatatc agcctatgga ttgaaaaaaa actcatctga aagagctgtt gctgctcgta
    14821 ataaagctga ggagttagta agcgcagcat tatatagcag accagagtta ttatcacagg
    14881 ctttgtctgg taaaacagta gatttaaaga ttgtttcgac ttctcttctg acgccgacca
    14941 gtttaaccgg gggagaggag agtatgttaa aagatcaggt gaacgcatta aaagggttaa
    15001 actctaaaag ggggaagcca acgaaattat taattcggaa tagtgatggt cttctgaaag
    15061 aagtaagcgt taatctaaaa gtggtgacat ttaactttgg tgtaaatgaa ttagcgctaa
    15121 aaatgggctt aggctggagg aatgttgaca agcttaatga tgaatcaata tgttctttgt
    15181 tgggggataa tttcctcaaa aatggtgtga ttggaggttg ggctgcagaa gcaatagaaa
    15241 aaaatccacc atgtaagaat gatgtgatat atctggctaa ccagataaaa gagattgtaa
    15301 ccaaaaagtt acaaaaaaat gataatggag agccgtataa attgtcacaa agaatgactc
    15361 tattggctta tactataggt gctgtacctt gctggaattg taagagtggg aaggacagaa
    15421 caggtatgca ggatgctgaa attaaaagag aaataataag aaaacatgaa acaggtcagt
    15481 tttctcaatt aaatagtaaa ttatcctcag aagagaaaag attattttct actattctaa
    15541 tgaatagtgg taatatggaa atccaagaga tgaatactgg tgtgcccgga aataaagtca
    15601 tgaaaaaatt gccgctatct tcgttagagc tatcttattc tgaaagaata ggggacccaa
    15661 aaatatggaa tatggtgaaa gggtattcgt catttgtata agaggaatat atggaagatt
    15721 tagcagatgt tatttgccga gccttgggta tccctttaat tgatatcgat gatcaagcaa
    15781 tcatgcttga tgatgatgtg ctaatatata ttgaaaaaga aggagattca ataaatttat
    15841 tatgcccttt ttgtgcattg cctgaaaata taaatgatct tatatatgcg ttgagcctaa
    15901 attactcaga aaagatatgc ttagctactg atgatgaagg cggaaatcta attgcgcgat
    15961 tagatcttac cgggataaat gaattcgagg atgtatatgt taatacagaa tattatattt
    16021 cgcgtgtgcg ttggctgaag gatgaatttg cgcgaagaat gaaggggtat taatgtcccg
    16081 tttcgtattt attttgctgt gttttatccc atatttaggg agagctgatt gttgggataa
    16141 ggctggtgaa aggtacaata ttccatcaag tttgttaaaa gcgattgcgg aaaaagaatc
    16201 cggatttaat aaatctgcag tcaatgttaa taacaatgga agtaaagatt atggtataat
    16261 gcaaataaat gactttcatt ctaaaagact tagagaaatg ggatattctg aggaaatgct
    16321 aattagtcat ccatgtcttt ctgtacatta tgcagcaaag ttattaaatg agtttatgat
    16381 gatgtatgga agagggtggg aggctgttgg agcctataat gcaggaacgt ctccgaaaaa
    16441 gaaaaaagaa agattaaagt acgcagagga tatctataga cgctatttgc ggatagcagc
    16501 agagagcaag cagaataatc gaaggatata agaggattta tgtctgaggc aaagaactca
    16561 aatcttgcac cattcaggtt attagtgaaa ctcacaaacg gtgtcgggga tgagtttccg
    16621 ttatattatg ggaataactt aattgttctc ggacggacga tagaaacact tgagtttgga
    16681 aacgacaatt tcccagaaaa tattattcct gtaacagata gtaaatcgga tggtataatt
    16741 tatttgacca taagcaaaga taatatctgt caatttagtg atgaaaaagg tgagcaaatt
    16801 gatatcaatt cacaatttaa ttcatttgaa tatgacggga tttcttttca ccttaaaaat
    16861 atgagggagg ataaatcgag aggccatata ctaaacggaa tgtataagaa ccactcggtg
    16921 ttttttttct ttgctgttat tgttgtgtta attataattt ttttcctctc tttaaagaaa
    16981 gatgaagtaa aagaaattgc tgaaattata gatgataaga gatatggaat agtcaacact
    17041 ggacagtgca attatattct tgccgaaaca caaaatgatg cggtatgggc gtctgtagcc
    17101 ttaaataaaa ccggatttac caaatgcaga tatattctgg tgtcaaacaa agaaataaat
    17161 agaattcaac aatatatcaa tcaacgattc ccatttatca atttgtatgt gttgaattta
    17221 gtgagtgata aagcggaatt actggtgttt ttaagtaaag aaagaaatag ttcaaaagat
    17281 accgaacttg ataaattgaa aaatgctctg attgttgaat ttccgtatat caaaaatatt
    17341 aaattcaact atcttagcga tcataatgca aggggggatg ctaaaggtat tttcacaaag
    17401 gtaaatgtcc agtataaaga gatttgtgaa aataataaag ttacatattc tgtcagagaa
    17461 gaactgacag atgaaaagct tgagctcatt aatagactga tttctgaaca taaaaatata
    17521 tatggagatc agtatattga gttttctgta cttttgatag atgatgattt taaaggtaaa
    17581 tcatatctta acagcaaaga cagttatgta atgttgaatg ataaacactg gtttttttta
    17641 gataaaaata agtgaggata aaatgagtgt tacagtaccg aatgatgatt ggacattgag
    17701 ttcattatct gaaacttttg atgatggaac tcaaacatta caaggtgaac taacattggc
    17761 actagataaa ttagctaaaa atccttcgaa tccacagttg ctggctgaat accaaagtaa
    17821 attatctgaa tatacattat ataggaacgc gcaatccaat acagtgaaag tgattaagga
    17881 tgttgatgct gcaattattc aaaacttcag ataataggga gcattcatga attacattta
    17941 tccagtcaat caggttgata ttatcaaagc cagtgatttt caatctcaag agatatcaag
    18001 tctggaagac gtcgtgtcgg ctaaatatag tgatattaag atggatacag atattcaagt
    18061 atcacaaata atggagatgg taagcaatcc agaatcatta aacccagaat ctttggccaa
    18121 gttacagacg acgctctcaa attattcaat aggagtatca ttagctggca cgttagcaag
    18181 aaaaacagtt tcggctgttg aaactttatt aaagtcttaa tttatatgat taggtataaa
    18241 ggttttattt tattcttgtt gctgatgttg attggatgtg agcaacgtga agagttaatt
    18301 tctaatttat ctcaaagaca ggcaaatgaa ataatatctg tgctagaacg ccataatatt
    18361 actgctagaa aagttgatgg aggtaaacag gggatctcgg tacaagtcga aaaggggaca
    18421 tttgcatcgg cagttgattt gatgcgcatg tacgatttgc caaatccgga gagagttgat
    18481 atctcacaaa tgtttcctac agattcatta gtgtcttctc caagagctga aaaggcccgt
    18541 ttatatagtg ctattgagca acggctggaa cagtctttag tttctattgg tggtgttatt
    18601 tcggcaaaaa tacatgttag ctatgatctt gaagaaaaaa atatatcttc aaaaccgatg
    18661 catatatcag taatcgctat atatgactca ccgaaagagt ctgaactatt agttagtaat
    18721 attaagcgat ttttgaaaaa caccttttct gatgttaagt atgaaaatat atctgtcata
    18781 ttaactccga aagaagaata tgtttataca aatgtacaac ctgttaagga agttaaatcg
    18841 gaatttttaa caaatgaagt aatatattta tttctcggga tggctgtact agttgtcatt
    18901 cttttggtat gggcattcaa aacagggtgg ttcaagagaa acaaaatatg ataagaatgg
    18961 atggaattta taaaaaatat ctttcaataa tttttgatcc agcgttctat ataaatagaa
    19021 atcggttgaa tttgccttct gaactgttag aaaatggcgt aatcagaagt gagattaata
    19081 atctcataat taataaatat gatctaaatt gcgatattga acctttaagc ggggtaaccg
    19141 ctatgtttgt tgccaactgg aatttacttc cagctgttgc ttattttatc ggtagtcaag
    19201 agtctcggtt aattaatcat agtgagatgg ttattagtta ttatgggggc aaaatcagta
    19261 agcaagggga ggccgctatt cgaagtggtt tttggcatct tattgcgtgg aaggaaaata
    19321 tatctgttgg tatatatgaa agaataaact tattatttaa tccaattgct ttggagggaa
    19381 attatacgcc tgtagaaaga aatttgtccc gattaaatga aggtatgcaa tatgcaaaaa
    19441 ggcactttac cggtatccag acatcatgcc tatgatggtg ttgttataaa gagaattgaa
    19501 aaggaattat gtaaaaccat aaaagataga gacactgaga gtaagaagaa agcaatttgt
    19561 gttataaagg aggctacgaa aaaagctgaa tcactgcgta tagatgctgt atgtgatggt
    19621 tatcaaattg gtatacagac tgcttttgag catattattg actatatttg tgaatggaag
    19681 ttaaagcaaa atgaaaatag aagaaatatt gaggactata taactagttt gctttcagaa
    19741 aatttacatg atgagaggat cattagcact ttattggagc agtggcttag ttcgttgcgt
    19801 aatacagtaa cagaattaaa agtggttttg cccaagtgta atttagcact gaggaaaaag
    19861 ttagaacttg atctccataa atatcgctca gatgtcaaga ttattttaaa atattctgaa
    19921 gggaataatt atatattctg tagtggtaat caagtagtgg agtttagtcc tcaggatgta
    19981 atatcaggtg ttaaaataga acttgcggaa aaattaacaa aaaatgataa aaaatatttc
    20041 aaagagttag cacataagaa actgagacaa attgcagaag acttgttaaa agagaatcct
    20101 gttaatgatt aatcaaataa atgcaagcaa tgctctgcag caaagattaa atagtgaaga
    20161 agttgttaat ttaaatgaga gattatcatc ttcgcaatca tttgatgagg atataatcta
    20221 tgaaataatg caatatttct cacagagtga attaaattcc atagataatg atgaactgca
    20281 caataagatt gaacaattat tcaattcacg ttttccttat cttacagctg cacaaaaaag
    20341 ttcacttctt aataaactca tagatgcaaa tcaatatgtc gatttgcatg agggatttta
    20401 tgcatcattg tccatatata acaatattga tttctatatt aagactacaa ccttcgattc
    20461 attaattagt gtctttgaag cagggagaga ggcagatgat tcgacatggt agtaataagt
    20521 tgaaaatatt tattttaagt atattgctat taacactgag tgggtgtgct ttaaagtcat
    20581 catctaattc tgaaaaagaa tggcatattg ttcctgtaag taaggattat ttttctattc
    20641 caaatgattt attatggtcg tttaatacaa ccaataaaag tataaatgtt tactctaaat
    20701 gtattagtgg taaggcggtt tatagtttta atgcaggtaa attcatgggc aactttaatg
    20761 ttaaggaagt agatgggtgc ttcatggatg cacaaaagat agctatagat aaactatttt
    20821 ctatgctgaa agacggggtt gttttaaaag gtaataagat aaatgatacc atccttatag
    20881 agaaggatgg ggaagttaaa ttaaaattaa ttcgagggat ataattgtat tgtgagtaaa
    20941 tataaaggtc taaatacaag taatatgttt tacatttact ctagtggaca tgaaccagtt
    21001 aacgttgagc ttgtaaaaga taaagaacgt aacataattg agctggctcc agcatggaag
    21061 ggtttttttt tgtccgaaat caaaatataa aattcagtga taatgtgaac tatcattatc
    21121 gctttaacat taatagttgt gcaaaatttc ttgctttttg ggactatttc agtggtgctc
    21181 tagtagagca tagccatgct gaaaaatgta ttcatttcta tcatgagaat gatcttcggg
    21241 atagttgtaa taccgaatcg atgcttgata agttaatgtt aagattcatc ttttcatctg
    21301 accagaatgt aagtaatgca ctggctatga tacgaatgac tgagtcatat catttggttc
    21361 tttatcttct gagaactatt gagaaagaaa aggaggtaag aataaagagt ctgacagagc
    21421 attatggtgt gtcggaggct tattttagat ctttatgtag aaaagcttta ggggcaaaag
    21481 tcaaagaaca actgaacact tggcggttag taaatggttt attagatgta tttttgcaca
    21541 atcaaacaat cacttcagct gccatgaata atgggtatgc atcaacatcc catttttcaa
    21601 atgagataaa aacacgacta ggcttttctg caagagaatt atcgaatatt acttttttag
    21661 tgaaaaaaat aaatgaaaaa atttaatatt aaatctttga ctctcttgat tgtattgtta
    21721 ccattgatag ttaatgctaa taatattgac tctcatttat tagaacaaaa tgacatagcg
    21781 aaatatgttg ctcaaagtga tacggtagga tctttttttg aacgtttttc tgcgttatta
    21841 aattatccta ttgttgttag caaacaagct gcaaaaaaga gaatatctgg ggagtttgat
    21901 ttatctaatc cagaagaaat gcttgaaaaa ctgacattac ttgtgggact catatggtat
    21961 aaagatggaa atgcattata catatatgac tctggagaat taataagcaa agttattttg
    22021 ctggagaata tatcacttaa ttatcttatt cagtatctga aggatgctaa cctttatgat
    22081 cacagatatc ctataagagg aaacattagt gataaaacat tctatatatc tggtcctccg
    22141 gcattggttg aactggtagc taatacagca actttgcttg ataagcaggt ttcttctatt
    22201 gggacagata aagttaattt tggggttata aaattaaaaa atacatttgt tagtgacaga
    22261 acttataata tgagagggga ggatattgtc atcccagggg tggctactgt tgtagaacgt
    22321 ttattaaata atggtaaggc tctgagtaac agacaagccc aaaatgatcc gatgccaccc
    22381 tttaatatta cacagaaagt atctgaggat agtaatgact tttcatttag ttcagttacc
    22441 aatagttcta tattagagga tgtatcactt attgcatatc cagaaaccaa ttctatatta
    22501 gtcaaaggta atgatcagca aatacagatt attcgcgata ttatcactca gttagatata
    22561 gccaaacgac atatagaatt atcattatgg ataatcgata ttgataaatc tgaattaaat
    22621 aaccttggtg ttaattggca aggaacggct tcatttggtg attcgtttgg tgcgtcattt
    22681 aatatgagct catcagcatc gataagcact ttggacggca ataagtttat tgcttcagtt
    22741 atggcattaa atcagaaaaa gaaagcaaat gtagtttcaa ggccggtgat tttgacacaa
    22801 gagaatattc cggctatatt tgataataac aggacttttt atgtgtcatt agttggtgag
    22861 cgcaattctt cgttggagca tgttacgtat ggaacattga taaatgttat acctcgcttt
    22921 tcatctcggg gacaaattga gatgtcttta acgatagagg atggaacagg aaatagtcag
    22981 tcaaattaca attataataa tgaaaataca tctgtccttc cagaagttgg aaggaccaaa
    23041 atcagtacaa tagcgagagt tccacaaggc aaaagtttgt tgataggtgg ttatacgcat
    23101 gaaacgaact ctaacgaaat aatatctatt ccttttctct cctctattcc tgtaatcggt
    23161 aatgtgttca agtataaaac aagtaatatc agtaatattg taagggtttt tctgattcaa
    23221 ccccgtgaga taaaggagtc aagttattat aatactgcag aatataaaag cctcatatct
    23281 gaaagagaaa tacaaaagac tacacaaata atcccatctg aaactacttt gctggaagac
    23341 gaaaaatcat tggtttcata cttaaattac taactataaa gtaggtgatg tatgcttgat
    23401 gttaaaaata caggagtttt tagctctgca ttcattgata agttgaatgc aatgacaaat
    23461 tcagatgatg gagatgagac tgctgatgca gagcttgatt ctggcttggc taatagcaag
    23521 tatattgact catctgatga gatggcttcc gctctttcgt catttataaa cagaagagac
    23581 cttgagaaac tgaaaggaac aaatagtgat agtcaggaac gtattttaga tggggaagaa
    23641 gatgaaatta atcacaagat ttttgattta aagagaacgt taaaagataa ccttcctcta
    23701 gatcgggatt ttatagacag actaaagaga tattttaaag atccaagtga tcaagtctta
    23761 gcattaaggg aacttttgaa tgaaaaagat cttactgctg aacaagtcga attattaact
    23821 aaaattatta atgagataat atcaggtagt gaaaaaagtg ttaatgctgg aataaattca
    23881 gctatacagg ctaaattatt tggcaacaaa atgaaacttg aaccacagct tttgcgtgca
    23941 tgttatcgtg gttttatcat ggggaacata tcaacaacag atcagtatat agaatggctt
    24001 ggtaattttg gttttaatca cagacataca attgtgaatt ttgtagagca gtcactgatt
    24061 gtagacatgg attctgagaa accgagctgt aatgcttatg agtttggttt tgtgttatct
    24121 aaattaattg caattaagat gattagaact tcagacgtaa tttttatgaa gaaactggaa
    24181 tcctcaagct tgctaaaaga tggcagttta agtgcagagc agctattgct aactttatta
    24241 tatatttttc aatatccaag tgaaagtgag caaattctta cttctgttat agaagtatca
    24301 cgagccagtc atgaggattc tgtagtgtat caaacatatc tatcttctgt taatgaaagt
    24361 cctcatgata tatttaaaag tgaaagtgaa agagaaattg cgatcaatat tctacgagag
    24421 cttgtcacaa gtgcatacaa gaaagagctt tctagataac aggagataaa agtgatccag
    24481 tcttttctta agcaagtaag tactaagcct gaattaatta tattagtgct tatggttatg
    24541 atcattgcta tgttgatcat accattacct acataccttg ttgatttttt aatagggctt
    24601 aatattgtac ttgctattct cgtttttatg ggatcatttt atattgaaag aatattgagt
    24661 ttttctacat tcccttcggt tttgttgata acgacattat ttcgtctcgc tttatcaata
    24721 agtactagtc gactaatttt agttgatgcg gatgcgggta aaattattac tacatttggc
    24781 cagtttgtca ttggtgatag tctggctgta ggttttgtta ttttttcaat agtaactgtt
    24841 gtgcagttca ttgttataac aaaaggatct gaaagggttg cggaggtagc tgctcgcttt
    24901 tctcttgacg gtatgccggg gaaacaaatg agcattgatg cggatttaaa ggctggaatt
    24961 attgatgccg caggagctaa agaaagacgc agtattctgg aacgtgagag tcaattgtat
    25021 ggttcatttg atggagcaat gaagttcatt aaaggtgatg caattgctgg catcattatt
    25081 atttttgtca atttaatagg aggtatttct gttggtatga gtcaacatgg aatgtcccta
    25141 tctggtgcat tatctactta taccatacta actattggtg acggattggt ctctcagatt
    25201 cctgctctgt taatttctat aagtgcagga tttatcgtta caagagttaa tggtgatagc
    25261 gataatatgg gacgtaacat aatgtcccag atatttggga atccttttgt tctcattgtt
    25321 acatcagctc ttgccttggc gatagggatg ttgccaggtt ttccgttttt tgtttttttc
    25381 ctgatagcag ttactttgac ggctttattc tattataaaa aggtcgtaga aaaagaaaaa
    25441 agtctgtctg agtctgattc tagtggctat actggtacat ttgatattga taatagccat
    25501 gactcatctt tggcaatgat agaaaatctg gatgctatta gttcagaaac cgttccttta
    25561 attttattat ttgccgaaaa taagataaat gcaaatgata tggaaggtct tattgaaagg
    25621 ataagaagtc agttctttat tgattatggt gtcaggcttc caactatttt atatagaaca
    25681 agcaatgagc ttaaggtcga tgatattgtt ttgttaataa atgaggtgcg tgccgatagt
    25741 tttaatatat attttgacaa agtgtgtatt acagatgaaa atggagatat agatgctcta
    25801 ggtattcctg ttgtttcaac ttcatataat gaacgtgtta tttcttgggt agacgtttca
    25861 tatacagaaa atctaactaa tattgatgct aaaattaaaa gtgctcaaga tgaattttat
    25921 caccagttgt cacaagcttt attaaacaac ataaatgaga tttttggtat acaagaaaca
    25981 aaaaatatgt tagatcagtt tgaaaatcgg tatcctgatc tattaaagga agtcttccga
    26041 catgtgacta tacagagaat ttctgaggta ttacaaagat tgcttggaga aaatatttct
    26101 gttcgcaatt taaaacttat tatggagtct ttggcgcttt gggctcccag agaaaaagat
    26161 gtcataacat tagttgaaca tgtccgtgca tcactttcta ggtatatttg tagtaaaata
    26221 gctgtttctg gtgagattaa agttgtgatg ctttccggat atattgagga tgcaataaga
    26281 aaagggataa ggcaaacctc tggtggctct ttcttgaata tggatataga ggtttcggat
    26341 gaggtaatgg aaactttagc acatgctttg agagaattga gaaatgcaaa aaaaaatttc
    26401 gttcttttgg tatcagtaga tatacgtagg tttgttaaaa gacttataga taacagattt
    26461 aagagtatac tcgttatatc gtatgctgag attgatgaag catataccat taatgtatta
    26521 aagactattt agtgaggttt aaatatgagt aacattaatt tagttcaatt agttagagat
    26581 agtcttttca cgattggttg tcctccatca attattacag atttagacag ccattcagcg
    26641 attaccattt ctttggatag tatgcctgct attaatatag ctcttgtcaa tgagcaggtt
    26701 atgttatggg ctaattttga tgcaccaagt gatgtaaaac ttcagtcatc tgcttataac
    26761 atattaaatt taatgttaat gaactttagt tatagcatta atgagctggt agagcttcat
    26821 cgttctgatg agtacttaca gttaagagtt gttattaaag atgactacgt tcacgatggg
    26881 attgtttttg cagagattct gcatgagttt tatcagagga tggaaatctt aaatggggtc
    26941 ttataatcaa tgagctatac aaaattgctc actcaattat cttttcctaa tagaatctcg
    27001 gggccaatct tggaaacaag tcttagcgat gtttcgattg gtgagatttg taacattcag
    27061 gctggaattg aaagtaatga aattgttgca agagctcagg ttgtaggatt tcatgatgaa
    27121 aaaacaatat taagcttgat tggaaattct cgtggacttt cacggcaaac gttgattaag
    27181 cccactgccc agtttcttca tacgcaagtc ggccgtggat tattgggagc agtagtcaat
    27241 cctttagggg aggttactga taagtttgct gttacagata acagtgaaat tctttatcga
    27301 cctgtagata atgctcctcc gctatatagt gaaagggctg caattgagaa gcctttttta
    27361 acaggtatta aggttattga ttctttactc acgtgtggtg aaggacagcg aatggggatt
    27421 tttgcgtcag ctggttgtgg caaaactttt ctcatgaata tgctcattga acatagtggt
    27481 gctgatatat atgttattgg gttaattggt gagcgaggtc gagaggttac tgaaacggtt
    27541 gattatttga aaaactctga gaaaaaaagc aggtgtgttt tagtatatgc aacttcggat
    27601 tactcttcgg ttgatcgttg taatgctgca tatatagcca ctgctatagc cgaatttttt
    27661 aggactgaag gacataaagt agcgcttttt attgattcat taacaaggta tgccagagca
    27721 ttacgtgatg tggccttagc cgctggagaa tcacctgcca gaagaggcta tccggtttcg
    27781 gtttttgata gcttacccag acttcttgaa aggccaggaa agttaaaggc aggtggctct
    27841 attactgcat tttacactgt tcttttggag gatgatgatt ttgctgatcc attagctgaa
    27901 gaggtaagat ccattttaga tggacatata tatttgagca gaaatctagc ccaaaaagga
    27961 caatttcctg caattgattc cttaaaaagt ataagcaggg tatttacaca ggttgttgat
    28021 gaaaaacatc gtattatggc cgctgcattt cgggagctac tttctgaaat agaagagcta
    28081 aggacaatta ttgactttgg tgaatacaaa ccgggggaga atgcctctca ggataaaatc
    28141 tacaacaaaa tatctgttgt tgagagtttt ctgaagcaag attatcgact cggttttact
    28201 tatgagcaga caatggagct tattggtgaa acaattagat aaggttttaa aaatcaaaga
    28261 taaatatcaa cggtctgtta aattaattga agcacacata ttaacactaa aaaaaaaact
    28321 ctttgtatcg agatgtggag gcattagata aaaggattat atacttcctg caactggaga
    28381 atgacttaga acctgtaggc gctcagagtg ttagtcagct tttcaataca agacgtaaga
    28441 tcgctatagt aaaaaagcat attatccaat atcagtctga acgaattctt ctaaagggaa
    28501 gaattgaaga aattcaaaaa gatattgatg aggcaaatgc ttctaaaaga aaactgcttc
    28561 ataaagagag taaaatatgt aaacggattg gcttgattaa aagaaataac tttgccaaac
    28621 agttgatact tgatgagtta tcacaagagg atatgaagta tggcattaga taatataaac
    28681 ctaaatttta gtagtgacaa acagatagaa aaatgtgaga aactatcttc aatagataat
    28741 attgatagtc tcgttttgaa gaagaagagg aaggttgaaa ttcctgagta ctctttaatt
    28801 gcatctaact attttactat tgataagcac tttgaacata agcatgataa aggagaaatt
    28861 tatagtggca ttaaaaatgc gttcgaactt agaaacgaac gagcgacata ttctgatatt
    28921 ccagaatcaa tggccattaa agaaaatatt ttgataccag atcaagatat caaagcaagg
    28981 gaaaaaataa atatcggcga tatgaggggg atcttttcat ataataagag tggaaatgca
    29041 gacaagaact tcgaaagaag tcatacttct tctgtaaacc ctgataatct gctagaatct
    29101 gataatagaa atggtcaaat tggtttaaaa aatcatagct tgtctattga taagaatatt
    29161 gctgacatca tttctttact aaatggaagt gttgctaaat catttgagct gcctgtaatg
    29221 aataaaaata ctgcagacat aaccccatcc atgtcattgc aagaaaaatc aatagttgaa
    29281 aatgataaaa atgtttttca aaaaaatagt gaaatgactt accactttaa acagtggggg
    29341 gctggacatt ctgttagtat ttcagtggag tctggttctt ttgttctaaa accgtcagat
    29401 caatttgtag gaaataaact tgacttaatt ttgaaacaag atgctgaggg taattacaga
    29461 tttgatagca gtcaacataa taaggggaat aaaaataata gtacaggata taatgaacag
    29521 agtgaagaag aatgctaaga attaaacatt ttgacgctaa cgaaaaacta cagattttat
    29581 atgcaaagca actctgcgag cgtttttcga ttcagacatt caaaaataaa tttacaggca
    29641 gtgaaagttt agtcactctt acttccgtgt gtggggattg ggtaattcgt attgatacat
    29701 tatctttttt gaaaaaaaaa tacgaggtat tttcaggatt ttctacacaa gaatctttac
    29761 tgcatttatc aaaatgtgtc tttatagagt cgtcatctgt attttcgatt ccagaactgt
    29821 ctgataagat tactttccgg atcacgaatg aaatccagta tgcaactact gggagtcatt
    29881 tatgctgttt ttcctcttct ttaggtatta tttattttga caagatgccg gtattacgta
    29941 atcaagtttc tcttgactca ttgcatcatc ttttagagtt ttgcttaggt tcatctaatg
    30001 taaggctggc tactttaaaa agaattcgca ctggtgatat aatcatagtt cagaaacttt
    30061 ataatttatt attgtgtaat caagttatta ttggggatta tattgtgaat gataataatg
    30121 aggcaaaaat taatctgtca gaaagtaatg gtgagtcaga acacacagaa gtttctttgg
    30181 cattattcaa ttatgatgat atcaatgtaa aagtggactt tattctttta gaaaaaaata
    30241 tgacaatcaa tgaactaaaa atgtatgtag aaaacgaatt attcaagttt cccgatgaca
    30301 tagttaaaca tgtaaatatt aaagtaaatg gttctttggt tgggcatggg gaacttgttt
    30361 ctattgagga tggttatggt atcgagatta gttcttggat ggtaaaggag taatctcatg
    30421 ctgagtgaca tgtccctcat cgctacactt tcttttttta cgctattacc atttttagtt
    30481 gctgctggta cctgttatat aaagttttct attgtttttg taatggtacg aaatgcactt
    30541 ggtttgcagc aggttccatc aaatatgact ctcaatggta ttgcattgat tatggctttg
    30601 ttcgtgatga aacccattat tgaagctggt tatgaaaatt atctaaatgg ccctcaaaaa
    30661 tttgatacaa tatctgatat tgtccgtttt tctgattctg gcttgatgga atataagcaa
    30721 tacttgaaaa agcatacaga tcttgagtta gcacgttttt tccaaaggtc tgaagaagaa
    30781 aatgctgatt taaagagtgc agagaataat gattattcat tgttttccct cttgcctgca
    30841 tatgcgctaa gtgaaatcaa agatgcgttc aaaataggtt tttatttata tttgccattt
    30901 gttgttgttg atcttgttat ttcaagcatt ttacttgcac ttggtatgat gatgatgagt
    30961 ccaatcacaa tatcagtacc aataaaactt gtattatttg ttgcgcttga tgggtggggt
    31021 atcctgtcta aggccttgat tgaacaatat atcaatattc ctgcttagat gatggaggta
    31081 atgtgtctga tatagtttat atgggtaata aggctcttta tttaatcctt atcttttcct
    31141 tatggccagt aggtatagct acggttattg gattatctat tggtttgtta cagacagtga
    31201 ctcaactcca agagcagaca cttccttttg gtataaagct tataggtgtc tcaatatctt
    31261 tgctacttct ttctggatgg tatggtgagg ttttattgtc tttttgtcat gaaataatgt
    31321 ttttaattaa gagtggggtt tgatggacat ttcaagctgg ttcgaaagta ttcatgtgtt
    31381 tttaatactc ctgaacggcg ttttttttag attggctcca ttatttttct ttcttccatt
    31441 tttaaataac ggtataattt ctccatctat tagaatacct gtgatttttc ttgttgcatc
    31501 aggattaatt acttctggta aggtagacat aggttcttct gtttttgaac atgtttattt
    31561 ccttatgttc aaggaaataa ttgttggcct ccttctctct ttttgcttgt ctcttccctt
    31621 ttggatattt catgctgttg gtagcattat tgacaaccag cgtggggcaa cgcttagtag
    31681 ttcaattgat cctgccaatg gtgttgatac gtctgagttg gcaaaatttt tcaatctttt
    31741 ttctgcagtt gtatttctat acagtggtgg tatggtcttt attttagaat ccatacaatt
    31801 gtcttataat atatgcccgt tattttctca atgttctttc cgtgtctcaa atatcttaac
    31861 atttctgact ttattggcaa gtcaggctgt tattttagcc agtcctgtta tgatagtatt
    31921 gttactatca gaagtattac ttggtgtatt atcgagattt gctccgcaga tgaatgcttt
    31981 ttccgtatca ttaactatta aaagtttact tgcaatattt attattttca tctgttcttc
    32041 tactatttac ttttctaaag ttcaattttt cctcggtgaa cataaatttt tcacaaatct
    32101 atttgttaga taaaatatta tggcaaataa aacagaaaag ccgacaccta aaaaactaaa
    32161 ggatgccgca aaaaaaggac agtcatttaa atttaaggat ttaacgactg ttgttattat
    32221 tctggtaggg acatttacta taatatcatt cttttcctta agtgatgtaa tgctcttata
    32281 cagatatgta ataattaatg acttcgaaat taatgagggt aaatactttt ttgcagtggt
    32341 tattgtcttt tttaagataa ttggcttccc actttttttc tgtgttcttt cggctgtgtt
    32401 gccaacattg gttcaaacaa agtttgttct tgcgactaaa gctatcaaga ttgatttttc
    32461 agtattaaac cctgttaaag ggttaaaaaa aatatttagt ataaagacaa taaaagaatt
    32521 tttcaaaagc attctgcttc ttattattct agcattaaca acctatttct tttggattaa
    32581 tgaccgaaaa ataatttttt ctcaggtgtt ttctagtgtt gatggcttat atcttatttg
    32641 ggggaggctg tttaaggata taatattatt tttcttggca ttttctattc ttgttattat
    32701 tcttgacttt gtgattgagt tcattttata catgaaagat atgatgatgg ataaacagga
    32761 gataaaaaga gaatatatag agcaagaggg acactttgag acaaagtcga gaaggcgtga
    32821 gttgcatatc gagattcttt cagagcagac taaatctgat atacgtaatt caaaattagt
    32881 ggtaatgaac ccgactcata ttgcaattgg tatttatttt aatccagaaa tagcgcctgc
    32941 accttttatt tctctcattg aaactaacca gtgtgccttg gctgtcagaa aatatgcaaa
    33001 tgaagttggt ataccgactg tgcgtgatgt gaaattagct agaaaactat ataaaacaca
    33061 tacaaaatat agttttgttg attttgaaca cttggatgaa gtcctacgtc ttattgtttg
    33121 gcttgagcag gttgaaaaca ctcattaata agtaaaggag atgtgttata tgggagttaa
    33181 tttctgtaat aaaataggta ttgatcagtc tgaatttgaa atagaatctt ccatcattaa
    33241 ctccattgct aatgaggtat tgaacccaat atctttcctt agcaataagg atataataaa
    33301 tgttttactc agaaagattt catctgaatg tgacctggtt agaaaagaca tttatcgttg
    33361 tgctctggag ttggtcgttg aaaaaacacc tgatgattta taatcattca taataactct
    33421 tttgctgata taaatatttt tgttgaagat atttatgtag tcacaggatc aattgtagaa
    33481 aattgttatg tttttgttag gaactgcact aaataatata tgataagaca gcaaaaacga
    33541 ctgaccataa tccttctttt attaggagtt gataagagag actattcatc ctgtaatgtt
    33601 aaaactctgt tatatagtat tcgcgattat gcaaagtctg taaatgatca tgagatacta
    33661 accgagagta atcgtttatt atctcattgc atttcagata gtaacggagc tttctttaaa
    33721 agtagtaaat acgttcccct taagtactta cgtaaaagga gaatagcacg aaagatacca
    33781 aatgattagc actttgctag taagcttgct ggtaaactat attgaccgtt gggtttgttt
    33841 tgagactgta atttaaattg tgtaattgcc tgtttttgat atgtttactc caacaacgga
    33901 gacaggtaaa ttatggacga aaagaagctt aaagcccttg cggctgaact ggctaaaggc
    33961 ctcaaaactg aggccgacct caatcagttt tcccgtatgc tgacgaaata tcccggctct
    34021 taaaacgata gtggctcagg ctttcatttt tcagcgttcc ccagaag
//
LOCUS       DAJTRN010000037        33950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRN010000037 DAJTRN010000000
VERSION     DAJTRN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(95..691)
                     /gene="ybjG"
                     /locus_tag="OO328_001818"
     CDS             complement(95..691)
                     /gene="ybjG"
                     /locus_tag="OO328_001818"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5930407.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            976..2202
                     /gene="mdfA"
                     /locus_tag="OO328_001819"
     CDS             976..2202
                     /gene="mdfA"
                     /locus_tag="OO328_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5930408.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(2243..2527)
                     /locus_tag="OO328_001820"
     CDS             complement(2243..2527)
                     /locus_tag="OO328_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930409.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(2613..3428)
                     /gene="ybjI"
                     /locus_tag="OO328_001821"
     CDS             complement(2613..3428)
                     /gene="ybjI"
                     /locus_tag="OO328_001821"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5930410.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(3428..4636)
                     /locus_tag="OO328_001822"
     CDS             complement(3428..4636)
                     /locus_tag="OO328_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5930411.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            4720..5256
                     /gene="rcdA"
                     /locus_tag="OO328_001823"
     CDS             4720..5256
                     /gene="rcdA"
                     /locus_tag="OO328_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5930412.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(5431..7116)
                     /locus_tag="OO328_001824"
     CDS             complement(5431..7116)
                     /locus_tag="OO328_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5930413.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            7386..7763
                     /locus_tag="OO328_001825"
     CDS             7386..7763
                     /locus_tag="OO328_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5930414.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(7793..8050)
                     /gene="grxA"
                     /locus_tag="OO328_001826"
     CDS             complement(7793..8050)
                     /gene="grxA"
                     /locus_tag="OO328_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5930415.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8210..8497
                     /locus_tag="OO328_001827"
     CDS             8210..8497
                     /locus_tag="OO328_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5930416.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            8481..9203
                     /gene="nfsA"
                     /locus_tag="OO328_001828"
     CDS             8481..9203
                     /gene="nfsA"
                     /locus_tag="OO328_001828"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5930417.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9264..10166
                     /gene="rimK"
                     /locus_tag="OO328_001829"
     CDS             9264..10166
                     /gene="rimK"
                     /locus_tag="OO328_001829"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5930418.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            10254..10730
                     /locus_tag="OO328_001830"
     CDS             10254..10730
                     /locus_tag="OO328_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5930419.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11082..12194
                     /gene="potF"
                     /locus_tag="OO328_001831"
     CDS             11082..12194
                     /gene="potF"
                     /locus_tag="OO328_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5930420.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVHENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12289..13422
                     /gene="potG"
                     /locus_tag="OO328_001832"
     CDS             12289..13422
                     /gene="potG"
                     /locus_tag="OO328_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5930421.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            13432..14385
                     /gene="potH"
                     /locus_tag="OO328_001833"
     CDS             13432..14385
                     /gene="potH"
                     /locus_tag="OO328_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5930422.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14382..15227
                     /gene="potI"
                     /locus_tag="OO328_001834"
     CDS             14382..15227
                     /gene="potI"
                     /locus_tag="OO328_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5930423.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            15287..15775
                     /locus_tag="OO328_001835"
     CDS             15287..15775
                     /locus_tag="OO328_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5930424.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            15816..16943
                     /gene="rlmC"
                     /locus_tag="OO328_001836"
     CDS             15816..16943
                     /gene="rlmC"
                     /locus_tag="OO328_001836"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5930425.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17118..17849)
                     /gene="artJ"
                     /locus_tag="OO328_001837"
     CDS             complement(17118..17849)
                     /gene="artJ"
                     /locus_tag="OO328_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5930426.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18141..18809)
                     /gene="artM"
                     /locus_tag="OO328_001838"
     CDS             complement(18141..18809)
                     /gene="artM"
                     /locus_tag="OO328_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5930427.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(18809..19525)
                     /gene="artQ"
                     /locus_tag="OO328_001839"
     CDS             complement(18809..19525)
                     /gene="artQ"
                     /locus_tag="OO328_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5930428.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(19532..20263)
                     /gene="artJ"
                     /locus_tag="OO328_001840"
     CDS             complement(19532..20263)
                     /gene="artJ"
                     /locus_tag="OO328_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5930429.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20281..21009)
                     /gene="artP"
                     /locus_tag="OO328_001841"
     CDS             complement(20281..21009)
                     /gene="artP"
                     /locus_tag="OO328_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5930430.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21227..21742)
                     /locus_tag="OO328_001842"
     CDS             complement(21227..21742)
                     /locus_tag="OO328_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5930431.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            22391..24160
                     /locus_tag="OO328_001843"
     CDS             22391..24160
                     /locus_tag="OO328_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5930432.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            24371..24694
                     /locus_tag="OO328_001844"
     CDS             24371..24694
                     /locus_tag="OO328_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5930433.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            24691..25521
                     /gene="amiD"
                     /locus_tag="OO328_001845"
     CDS             24691..25521
                     /gene="amiD"
                     /locus_tag="OO328_001845"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5930434.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(25518..26531)
                     /locus_tag="OO328_001846"
     CDS             complement(25518..26531)
                     /locus_tag="OO328_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5930435.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(26630..28060)
                     /locus_tag="OO328_001847"
     CDS             complement(26630..28060)
                     /locus_tag="OO328_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5930436.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28071..29072)
                     /gene="ltaE"
                     /locus_tag="OO328_001848"
     CDS             complement(28071..29072)
                     /gene="ltaE"
                     /locus_tag="OO328_001848"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5930437.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29109..30857)
                     /gene="poxB"
                     /locus_tag="OO328_001849"
     CDS             complement(29109..30857)
                     /gene="poxB"
                     /locus_tag="OO328_001849"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5930438.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGT
                     PTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLM
                     GDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITG
                     IRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIIS
                     GRGDEVIELAKTNWLR"
     gene            complement(30990..31958)
                     /gene="hcr"
                     /locus_tag="OO328_001850"
     CDS             complement(30990..31958)
                     /gene="hcr"
                     /locus_tag="OO328_001850"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5930439.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(31970..33622)
                     /gene="hcp"
                     /locus_tag="OO328_001851"
                     /gene_synonym="priS"
     CDS             complement(31970..33622)
                     /gene="hcp"
                     /locus_tag="OO328_001851"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5930440.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(33766..>33950)
                     /locus_tag="OO328_001852"
     CDS             complement(33766..>33950)
                     /locus_tag="OO328_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HCR5930441.1"
                     /translation="IPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMVPAAIVHG
                     FILSLLVPILIAFFSA"
BASE COUNT         8374 a         8407 c         8749 g         8420 t
ORIGIN      
        1 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
       61 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
      121 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
      181 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
      241 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
      301 gcaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
      361 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
      421 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
      481 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
      541 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
      601 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
      661 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
      721 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
      781 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
      841 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
      901 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
      961 tggcgaagaa attgcatgca aaataaatta gcttccggtg ccaggcttgg acgtcaggcg
     1021 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     1081 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     1141 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     1201 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     1261 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     1321 ggcataagct tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     1381 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     1441 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cttgggaggg gatgtttgtt
     1501 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     1561 gccatgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     1621 ctgaagaacg gccgctttgt ggcgggggcg ctgggattcg ttagcctgcc attgctggcg
     1681 tggatcgccc agtcgccgat tatcatcatt accggggagc agttgagcag ctatgaatat
     1741 ggcttgctgc aagtgcctat tttcggggcg ttaattgcgg gtaacttgct gttagcgcgt
     1801 ctgacctcgc gccgcaccgt acgttcgctg attattatgg gcggctggcc gattatgatt
     1861 ggtctgttgg tcgctgctgc ggcaacggtt atctcatcgc acgcgtattt atggatgacc
     1921 gccgggttaa gtatttatgc tttcggtatt ggtctggcga atgcgggact ggtgcgatta
     1981 accctgtttg ccagcgatat gagtaaaggt acggtttctg cggcgatggg aatgctgcaa
     2041 atgctgatct ttaccgtcgg tattgaaatc agcaaacatg cctggctgaa cgggggcaac
     2101 ggactgttta atctcttcaa ccttgtcaac ggaattttgt ggctgtcgct gatggttatc
     2161 tttttaaaag ataaacagat gggaaattct cacgaagggt aaaaaaatgc ctgattgatt
     2221 tgtgcgatca ggcattctcg aattaatggt gatggtcgtc aatctggtgt tcgataacca
     2281 tcccttcacc tacgctggca agatggcgaa cataaggatg cgggcggtaa gccggagctg
     2341 gcgcaggagc cacatagacg gtttgcggca cagtcgcgac gctgaccgct tgtggaacgc
     2401 tgactgaatc aggaaccaca accactttgt atccactcgg cacatcgacg gtgacacttt
     2461 gcgccatcgc cacaccagta aagcccatta aaagcgcgcc caacagtaga caatttttca
     2521 taatcatttc gcctgaaata atgcagcggg ttaatttccg cgaattatgc agccttcatt
     2581 caggcattgt gttgccgggc gtgctgccgt ttttattggt caaatggcgc ttcgtgttta
     2641 agaactttat cgatcacatc cagtacgcct tcacggttat tgtagcctgc ccggtatttt
     2701 gccgctgcga cgactgcgct gccggcattt tccattgcaa aactaaagcc tgcctgacgc
     2761 agcatctcaa tatcgttacc gccatcgcca aagaccacca cttcgctgtc gtctattccc
     2821 cataatttct gcagttggcg aaggccattg gctttatgta cgccggggat aatcagatcg
     2881 atgctgccgt tgccggtgtg gactggcacc ataatatcgc cgatggcctc atgtaatgct
     2941 ttttgtactt gtggaatcag ttcatcggaa agattcaggc caaacttaaa gaaaatatct
     3001 tctaagttgt caaagttatc gacgtattcc agacggtgat aatacatttc cgccaccgtt
     3061 ttcatggcat cgtcatattt tttgagtgta taggcgctat tttttccgca ggcaataatt
     3121 tccacttccg ggcgcgtcag caaatgttcc acgacagtag caaacgcatc cttcgacagc
     3181 tcgccattaa aaacatcttt gccttcactc attacccagc cgccgttttc agccacaaag
     3241 gcaatttcat tcgcaatttc agggaagaaa gagatcaact gataatattg attcccgctg
     3301 gcgaccacaa agcgaatgcc ttgtgctttc atttgctgat actgagccat aaaccgctca
     3361 cggttatagg ttttttgatc gcttaagaaa gtaccatcca tgtctaccgc aattaattta
     3421 atgctcatca actattctcc atcgccgtct gcgttttggt atcgggtttg gcgacggctt
     3481 tcgcgacaat agccgcgaga ataaccagcg ccagtacaac cagcattgca ctacgtaatc
     3541 cataatgttc gccgagatag cccagcagcg gcggcccgac gaggaaagcc agataaccgg
     3601 tcgttgctac cacactgacg cgggttggtg catcggggcc ggtatcgctg gcggcagaaa
     3661 tggtcagcgg gaagcccagc gaggcaccca gtccccaaag tacaacagac actcctgcga
     3721 cccaggcgct atcgacaaaa ataatcagcc caatacccaa cgcccccatt agtgcactgg
     3781 cccgaaccac ggcaacgcga ctgtaactgt cgatgaacca accgccggtg aagcgtccaa
     3841 cggtcatccc cagggtaaaa ccggcataaa tcagtgagcc ggaagtgggg ctaaaaccgt
     3901 gaccatcaac cattaataag ggtaaccagt cgttggcaga accttcggca aaggccatcg
     3961 ccagcactac aacaccaatc aacagcaact ggatatcgcg ataaaaagga atgccttttt
     4021 cgccatgctg ggtgccatcg gcagcatttt tgcccgtacc gtcagggatt gcctgaatgg
     4081 cgatataaat aggcgcgata cctaccagcg ccgccaataa aatgtgcacc gttgccggaa
     4141 cgccaaaggc cgtcagtgcc atcccgacac cagcgcctgc cagcgtgccc aggctataaa
     4201 aaccgtgcat catcggcaaa accgttttat tcatttctcg ctcaacggcg gcaccttcaa
     4261 cgtttatcgc cacttccgca gaaccaaaac ttgccccaaa gacgccgaga ccaacggcaa
     4321 agagcagggg cgatgtcagc cagagtgcca gacttaatat catcatcccg atcaatgcgc
     4381 aggacatcgt gaccaggatg acattacgtg tcccaaagcg tttcactaac cacgccgagc
     4441 agagaatacc gctcatcgaa ccgatcgaca gaccaaagag aacaccgccc atttcagcga
     4501 tcgagacaga gagaatatcg cggatagcag gcgtacgggt tgcccaggac gccattaaca
     4561 ggcctggcaa aaagaagaac ataaacagcg cccaggttcg gcgtttcaat gcattacgtg
     4621 aagaatttac ggtcatagat cacgtcaaaa taagaagagg gaagacaaca ttagcaaggt
     4681 tgtgtacatt tgtacacaat tgcagacaga ggaaatgaca tgcgtcgcgc taacgatccg
     4741 caacggcgag aaaaaattat ccaggccacg ctggaggcgg tgaaacttta cggaatacat
     4801 gctgttacgc accgcaaaat tgctaccctt gccggggtac cgttgggatc gatgacctac
     4861 tatttttcag gaattgatga gttgttgctg gaggcgttca gcagttttac tgagattatg
     4921 tcccggcaat atcaggcatt ttttagcgat gttagtgatg ctcaaggcgc atgccaggct
     4981 atcaccgata tgatctacag ctcacaggtt gcaacgccgg ataatatgga gctgatgtac
     5041 cagctctacg cgctggctag ccgaaaaccg ctattaaaaa cggtaatgca aaactggatg
     5101 cagcgcagtc agcaaacgct cgaacaatgg tttgaacccg gaaccgcccg cgcgcttgat
     5161 gcgtttattg aggggatgac gctgcatttt gtcaccgacc gtaagccgct atcgcgcgag
     5221 gagattttga ggatggttga gagggttgca gggtagtaga taaattttag ataacaaaaa
     5281 acccatcaac cttgaaccga aatggcgggg ttgatgggct ccacaaaatg gggacatcaa
     5341 agaaaagcag tggcactaat taagactgat gccctgcgga aaagttctgc ggttgtgcaa
     5401 aaaaatttca ttttcagggc aacttcagtt ttatcctaat cctggccata ccatgacgat
     5461 gattgtccct gccagcgtca gcaggacgtt ggcgattgcg taggtgcccg catagcccag
     5521 cgcagggatg ttactgcgag ctgtatcact gatgatctcc attgccggcg cgcaggtgcg
     5581 tgcgcccatc attgcgccga acaacagtgc gcggttcatt cgcaatacat aagcaccgaa
     5641 caagaaacag ataaccacgg gcacaagact gacaattaat ccggcaatca acatctgacc
     5701 gccaatcgcg cccaggccgt tattaatacc gctaccggcg ctcagaccaa cgcctgccat
     5761 aaacaccatc aagccgaact ctttcaccat gcttaatgca ccttgcggaa tgtaaccgaa
     5821 ggtcgggtgg ttagcacgca taaagcccag cataattccg gcgaataaca acccggcagc
     5881 gttccccatg ccgaaactga atgtgctgaa ctggaaggtg atcatcccga tcatcagccc
     5941 aataacaaag aaggcgcaaa atgccagcag gtcagtgacc tggctgtgaa tcgagataaa
     6001 gccgatgcga tcggcgatgg tttttacgcg acgggcatca ccgctgactt gtaaaacgtc
     6061 acctttgtta agcacgacgt tgtcatctat cggcatctca atctggctac gaatgacgcg
     6121 gttaaggaag caaccgtgat cggtcaactt cagttgtgcg agacgtttac ctacagcgtt
     6181 atggttttta acgaccactt cttcagtgac gatacgcatg tcgagaaggt cacgatcgaa
     6241 aacttcttta ccgttacgga agctgggatc gagtcgggca tgggcgtcgg gatagcctac
     6301 caacgctatt tcatcgccca tttgtagcac ggcatcaccg tctggatttg ccagaatccc
     6361 gttacgtcga atacgttcaa tgtagcagcc ggtttgtcga taaataccca gttcacgcag
     6421 atttttgccg tcggtccagg ccaccagctc cgggccgacg cgataggcgc ggatcaccgg
     6481 taaataaacc ttacggttgg catcagtgtc caggccacgt tcgcgggcga tttgctgggc
     6541 gctggtctgt aagtcctgat gctgcaattt cggcaagtaa cgcgcaccaa caatcaaact
     6601 caccagaccg attaaatagg ttaaggcata cccgaggctc agattatcca gtgccagtga
     6661 gagctgcctg ctttccatgc cggaatgatg cagtgtatcg ccagcaccga ccagaaccgg
     6721 tgtcgacgtc atagagcctg ctaacatacc ggccgtcagg ccaatatccc agccaaacag
     6781 cttacctaac cctaaggcga tcaccagcgc actgccaacc atcaccagtg ctaacattag
     6841 gtaatttttc ccatcgcgaa aaaaaatgga aaaaaagttc ggtccggctt cgaccccgac
     6901 gcagaaaata aacagcataa agccaagatt aagcgcatcg gtgttaatgc tgaaatgttg
     6961 ttggcctaat aacagcgata cgactaaaac gccaatggaa ttacccagtt ggatcgaacc
     7021 aagtcgtaac tttccgagac atagcccaag cgcgaggacc acaaataata acagaatgta
     7081 attcccattt aacaattcgg cgacgtttat attcacggag gctaacttct tgtttactag
     7141 taagctgttg aaagaaatgc taatttacga taatgtgttt taccagaatt cagggcgcag
     7201 attcattcag cgcacctaaa cgatagtaaa gtaacaatat attttactag tgtaatcaca
     7261 ttaggtatca acggctatat gaattgcgtt ggcctatatt agcatggaat gcgaagcggc
     7321 tttatcttac tgaacgccac actggcgaaa aatgtgttcg atagacgcag tgtcaggagg
     7381 aacgagtgaa acataaacaa cgttgggcgg gggcaatctg ctgttttgtc ctcttcattg
     7441 tggtgtgcct ttttctggcg acgcacatga aaggcgcttt tcgggctgcc gggcatcctg
     7501 aaatcggctt gctgtttttc attcttcctg gagcagttgc cagtttcttt tcacagcgta
     7561 gagaagtcct gaaacctcta tttggcgcaa tgctggcggc accctgttca atgctcatta
     7621 tgcggctgtt tttttcaccg acgcgctcat tctggcaaga gctggcatgg ttactaagcg
     7681 cggtgttctg gtgtgcgctg ggggcactgt gtttcttatt tatcagtagt ttgtttaaac
     7741 cacagcacag aaaaaatcag taaagccctc aacgcgaggg cttgtcagac gatcaggcgt
     7801 ccagattttc tttcacccat gcagcaaaat cggtatagcc gccgatatgt tgctgatcga
     7861 caaaaatctg cggcacggtt tctacgggtt tacctgcctt ttgttgtaga tcttctttag
     7921 tgatcccttc cgcacgaata tctacatact gatactgaaa atcatcgcgt tcattgctca
     7981 atttctcagc cagatctttt gcacgcacac agtaagggca acccgaacga ccaaaaataa
     8041 cggtttgcat tatttctctc ctcatagatt tatgcctgta atgatcacgc taaaatgtat
     8101 tcgctgaaag taggtttaac ctgttgcatt aattgctaaa agctataact gttaaacaca
     8161 atacagtgaa aagttttaga ctgaaggctc actttgcaga gggaagcgta tgcgcgcgat
     8221 cggtaaattg cctaaaggcg tgttgatact ggaatttatc ggaatgatgc tactggcggt
     8281 ggcgctgctg tcggtaagcg actccctgtc gctgcctgag ccattttctc ggccagaagt
     8341 gcagattctg atgatttttc tcggtgtttt gctcatgctt cccgctgcgg tggtggttat
     8401 tcttcaggtg gcaaaacgtc ttgccccaca gctgatgaac tgtccaccgc aatattcacg
     8461 ttcagaaaga gaaaaagata atgacgccaa ccattgaact tatttgtggc catcgcgcca
     8521 ttcgccattt cactgatgaa cccatttccg aagcgcagtg tgaggcgatt attaacagcg
     8581 cccgtgcgac gtccagttcc agttttttgc agtgtagtag cattattcgc attaccgaca
     8641 aagcattacg tgaagaactg gtgacgctga ccggcgggca aaaacacgta gcgcaagcgg
     8701 cggagttctg ggtgttctgt gccgacttta accgccattt acagatctgt ccggatgctc
     8761 agctcggcct ggcggaacaa ctgttgctcg gtgtcgttga tacggcaatg atggcgcaga
     8821 atgcattaac cgcagcggaa tcgctgggat tgggcggggt atatatcggc ggcctgcgca
     8881 ataatattga agcggtgacg aaactgctga aattaccgca gcatgttctg ccgctgtttg
     8941 ggctgtgcct tggctggcct gcggataatc cggatctcaa gccgcgttta ccggcctcca
     9001 ttttggtgca tgaaaacagc tatcaaccgc tggataaaga cgcactggcg cagtatgacg
     9061 agcaactggc ggaatattac ctcacccgtg gcagcaataa tcgctgggat acctggagcg
     9121 atcatatccg ccgaacaatc attaaagaaa gccgcccatt tattctggat tatttgcaca
     9181 aacagggttg ggcgacgcgc taaaaccgcc acgtcgatgt atgatacgcg ggcttttgac
     9241 caggtctgac agagaggtgc agggtgaaaa ttgccatatt gtcccgggat ggaacgctct
     9301 attcgtgtaa gcggctgcgt gaagccgcta tacagcgcgg tcacctggtt gaaattcttg
     9361 atccgctttc ttgctacatg aacataaatc ctgcggcgtc ttctatttac tacaaaggcc
     9421 gcaagttacc ccattttgac gcagtgatcc cgcgtattgg caccgccatt accttttatg
     9481 ggacggcggc actgcgccag ttcgagatgc tggggagcta tccgctcaat gagtcggtcg
     9541 ccattgcccg ggcgcgtgac aaattgcgtt ccatgcaact gctggcgcgt cagggcatcg
     9601 acctgcctgt cacgggcatt gcgcattcgc cggatgatac cagcgattta atcgacatgg
     9661 tcggtggtgc gccgctggtg gtcaagttgg ttgaaggcac gcagggaatt ggcgtcgtgc
     9721 tggcggagac tcgtcaggcg gcggaaagcg tgattgacgc tttccgcggt ctgaacgcgc
     9781 atattctggt gcaggaatat atcaaagagg cgcaagggtg cgatatccgc tgtctggttg
     9841 ttggtgatga agtggtcgct gcgattgaac ggcgggcgaa agagggctat tttcgttcca
     9901 atttgcatcg tggcggcgcg gcaagcgtcg ccagtatcac accacaggag cgtgaaatcg
     9961 cgataaaagc cgcgcgaacg atggcgctgg acgttgctgg tgtggatatt ctgcgtgcta
    10021 atcgcgggcc gttggtgatg gaagtgaatg cgtcgccggg gctggaagga atagaaaaaa
    10081 ccaccggtat cgatatcgcg ggtaaaatga tccgctggat cgaacgccac gctacgacag
    10141 aatattgcct gaaaacgggt ggttagtcgc aatcacatta ctgatcatgg ttttgcctgc
    10201 gctttttgcg taagctgtgc cggtcttttt atcgaaagag gttgtacaaa attatgacat
    10261 cgctggtcgt tcctggtctg gatacgctgc gtcaatggct cgatgacctg gggatgagtt
    10321 tttttgaatg tgataactgt caggctctgc atctgcccca tatgcagaat ttcgacggtg
    10381 tctttgatgc caaaatcgat ctgatcgata acacgatcct gttttctgcc atggcggaag
    10441 tccgaccttc agccgtattg ccgctggcgg cggatttatc tgccatcaat gccagttcgc
    10501 tgaccgtgaa agcatttctt gatatgcagg atgataatct gccaaagctg gtggtttgcc
    10561 agtctttatc cgttatgcag ggcgtaacct atgagcagtt tgcatggttc gtgcgtcaga
    10621 gcgaagagca gatttcgatg gtcattcttg aagctaatgc ccatcaactg ctgttaccga
    10681 ctgacgatga agggcaaaac aacgttaccg aaaactattt tctccactga taattccttt
    10741 cgagcacgca gtcgctgccg cagtgactgc gcgctgcaaa attatctgct gtttttaacc
    10801 ttttcttaaa gattatttca cttctcttgt gtcgatttgg ctttatcaca tagagcaaat
    10861 acgcataaaa atttgttaaa taccgttttt taatccgagc tatagtctca aaccctggct
    10921 aaagttattc ttgcgatgct tctatatagc gagcagtgct ggccgggaga gagttctctt
    10981 ttcttacacc gcgccgataa aaaaatatgc acgtttattg catatctttc agtgtgacaa
    11041 cttttgttcg tttgttaacg aactttcaga aggaaagaga tgtgaccgcc ttaaataaaa
    11101 aatggctatc gggtctggtt gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg
    11161 ctgaacaaaa aacactccac atttataact ggtctgatta tatcgcccct gacacggtgg
    11221 ccaattttga aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag
    11281 tactggaagg caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca
    11341 gctttctaga acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc
    11401 cggagtggaa gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca
    11461 ataaatttgc tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag
    11521 ttaaagctgt gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg
    11581 aaaatctgga aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt
    11641 ttgctaccgt gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca
    11701 ccggaccggc aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat
    11761 ctcaatacat taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg
    11821 atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct
    11881 cgattccaaa agaaggggcg atggcgttct ttgatgtatt cgccatgcca gcggatgcca
    11941 aaaacaaaga cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc
    12001 atatttccga ccatgtgttc tatgtcaacg ccaataaagc agccacgccg ctggtgagtg
    12061 cggaagtcca tgagaacccg ggtatttatc cgcctgcgga tgttcgtgcg aagctgttca
    12121 ctctgaaagt gcaggacccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga
    12181 agagcggaaa ataatccgca gccttagatg ccgggcgggc gcaccacacc cgccggcaat
    12241 tcgcaccatc atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct
    12301 atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac
    12361 ctcaccaaat cctacgatgg tcaacatgcg gtggatgatg tcagcctgac catctacaaa
    12421 ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    12481 ctggcaggtt tcgaacaacc ttctgccgga cagataatgc ttgatggcgt cgatttgtca
    12541 caggttccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    12601 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    12661 gaaatttcca gtcgggtcaa tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    12721 cgtaaaccgc atcagctttc tggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    12781 gccaagcgcc cgaaactatt actgctcgac gagccgatgg gcgcgctgga taaaaagctg
    12841 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    12901 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgcatcgc cattatgaat
    12961 cgtgggaaat ttgtccagat tggcgaaccg gaagagatct acgagcatcc gactacccgc
    13021 tatagcgccg aatttattgg ctcggtaaac gtctttgaag gcgtactcaa agagcgtcag
    13081 gaagatggct tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgacgcggat
    13141 gcctcggtgg tcgataacgt gccggtacat gtggcgctgc gcccggaaaa aatcatgctt
    13201 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    13261 tatctcggcg atctttcggt gtatcacgtt cgtctgaaaa gtgggcagat gattagtgcc
    13321 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    13381 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgaataca
    13441 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    13501 caaatgaagc atgggcgcaa actggtcatt gcgttgccat atatctggtt gatcttgctg
    13561 tttctgctgc catttctgat tgtctttaaa ataagcctgg cggagatggc gcgcgctatt
    13621 ccaccttata ccgaactgat ggagtgggct gacgggcaac tgtccatcac tcttaatctc
    13681 ggtaattttc tgcaactgac cgacgatccg ctctatttcg atgcttatct ccagtcgcta
    13741 caggtggctg cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg
    13801 gtggcgcaca gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg
    13861 tggacctcgt ttctgatccg cgtttatgct tggatgggaa tattaaaaaa caacggtgtg
    13921 ctgaataatt ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tcttcatacc
    13981 aatctggcag tttatattgg cattgtttac gcctacgtgc cgtttatggt gctgccgatt
    14041 tataccgcgt tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca
    14101 cgaccgctga aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc
    14161 ggatcgatgc tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt
    14221 ggcccggaca gcatcatgat cgggcgcgtg ctatggcaag agtttttcaa taaccgcgac
    14281 tggccggtgg cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg
    14341 tggttccaca aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag
    14401 ttcgttcgcc ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa
    14461 tgctgatgct ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tggggcggct
    14521 ggtcaacgcg ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt
    14581 caagcctgac aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg
    14641 cggtggtgct ggtgcgcttt ggcaggtttc gcggatcaaa tggctttgcc tttatgatca
    14701 ccgcgccgct agtaatgcca gatgtcatca cgggcttgtc gctgttgtta ttattcgtcg
    14761 cgcttgctca tgccattggc tggcctgcgg accgcggtat gcttaccatc tggctggcgc
    14821 atgtcacttt ctgtacggct tatgtggcgg tcgttatttc gtcgcgtctg cgggaactgg
    14881 atcgctcgat agaagaagcc gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg
    14941 tcattacgct accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt
    15001 tgtcgcttga tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac
    15061 cgatgctggt cttttccagc gtgcggatgg gggtgaatcc ggaaatcaac gccctggcaa
    15121 cattgatcct cggcgcggtc ggaattgtcg gatttatcgc ctggtatctg atggcgcgcg
    15181 cagaaaaaca gcggatacgc gacatccaac gtgcaagacg tggctgaaga cactaaaatt
    15241 tgccaacctg gctacataat gccgcgcatg tcgcggcatt gttttcatgg aagacgaaac
    15301 gttgggattt tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt
    15361 gcaggtggcg gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa
    15421 tacgctgggc gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt
    15481 aacgctggtc tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc
    15541 actggtgaaa gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc
    15601 aagttacttg tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg
    15661 tgaatcaaaa cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat
    15721 cctgatgctg ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt
    15781 ataatcgtcg cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg
    15841 gtcgctgtcg ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa
    15901 ccgccgatct taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt
    15961 caggcccgga acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa
    16021 aaacactgct cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc
    16081 tttatccagc ctcatttgcg cccgtttttg cggcgctaaa accgtttatc gcccgagcgg
    16141 ggttaacgcc ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg
    16201 aaagccagag tgatggcggc atgatgctgc gctttgtact gcgttctgat accaagctgg
    16261 cgcaactgcg taaggcgctg ccgtggttac acgaacaact accgcagctg aaagttatta
    16321 ccgtcaatat tcagccggta catatggcga ttatggaagg ggagacggag atctacctga
    16381 ccgaacaaca ggcactggcg gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa
    16441 gtttcttcca gactaatccg gcggtcgcca gccagttgta cgccaccgcg cgcgactggg
    16501 tacgacagct gccggttaaa catatgtggg atctgttctg cggtgtgggg ggctttggtt
    16561 tacactgcgc gacgcctgac atgcagttaa ccgggatcga aattgcgcca gaggccattg
    16621 cctgtgcaaa gcagtcagcc gctgaactgg gcttaactcg tttacaattt caggcgctgg
    16681 actccactca gtttgccact gctcaggggg aggtgccgga gctggtgctg gttaacccgc
    16741 cgcgccgcgg cattggtaaa ccgctgtgtg attatctttc aacgatggca ccgcgtttta
    16801 ttatctactc cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt
    16861 accgcatcga acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtgt
    16921 tgacgctgct ggtgaagcaa taaaaaagcc acaggtgcgg cttcagattg ctgatcaggt
    16981 gcgctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgctaa
    17041 atcaatacat tgcagagttg atgtgggccg gataagcgca gcgcatcagg caatcttact
    17101 tttgtcatca gtctcaatta ctgcggaaac cactggtcat tgattttctg atatgtaccg
    17161 tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga
    17221 cgtacagcga tacccaggcc ggtgccgaag tattgcggat cggtcacttt ttcagtcgca
    17281 acgcccagtt gcggattggt tttcagccat tcgtttacca ccgcggtgtc accaaatacc
    17341 ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca
    17401 gttttcactt ccgggtgctg atcctgaata tatttctggt gcgtcgtacc gttttccatc
    17461 ccaatgcgtt tgcctttcag gtcggcaaac gttttgtagg tatctttttt ggaaatcacg
    17521 acggctgagt tttcatagta gggtgtggta aacgaaacct gcttgctacg ctccggggtg
    17581 atatccatac cggagattac ggcgtcatat tttctgaatt tcagtgacgg gatcaggctg
    17641 tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc
    17701 agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag
    17761 gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca
    17821 agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt
    17881 tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaac
    17941 atgattaaat aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt
    18001 cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca
    18061 ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc
    18121 ggcgaatgta cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc
    18181 agacgcatca tcagcgtcag caggccgttg acgaccaagt aaataatccc tgccgcaccg
    18241 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc
    18301 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
    18361 gaagagagcg agcgtttaaa ggcatacggc agcaggatcg ccagcgtatc ttttttgctc
    18421 attcccaggg cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa
    18481 aacagctgcg tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac
    18541 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc
    18601 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa
    18661 ccccgcacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
    18721 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgcagccct
    18781 ttcatcagct cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
    18841 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
    18901 ccgccacaat gtaccaggta aatggttcct gggtacgagt agcaatgctt ttggtttgta
    18961 acattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
    19021 actggttacc gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa
    19081 agatagccga tttcgacagc cccagcgcct gaccagattc ccactggccc accggtacag
    19141 ctttcaacgc gccgcgcagc gtttgcgagg cataggcggc atacagcagt gacagagcga
    19201 tgacaccaca aaggaacgga ctcacgtcga agttctcaat gtccatctgc accgggatct
    19261 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagtaac tgcgaggagc
    19321 caaaatagat aaacagcact accagaattt ctggcaggcc acgcagaatg gttaccagcg
    19381 ctgaacctgc ccacgcgaca ggacgccatt tcgctgactc ccataccgca aagaacatcg
    19441 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
    19501 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
    19561 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagtt
    19621 cagtgtttcc ctgacgtacc gcgatgccga ggccagtacc gaaataatct ttatcggtca
    19681 ctttatcgcc cactgccgcc agttttgggt tatctttcag ccactcagtg accactgcgg
    19741 tgtcaccaaa tacgctgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc
    19801 tgtcatacgg aacggtggtg atttccgggt gcttatccat aatgaacttc tggtgtgtcg
    19861 tcccgttctg aacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
    19921 gctgacccac aaacagggca gagttgtcat agtacggggt agtaaacagt acctgctttt
    19981 cacgctccgg agtgatatcc atgcccgcca tcactgcttc tacgcgacgg aatttcaggc
    20041 ttgggatcag gctgtcaaac gcctggttag agaaagtaca ggttgcatca atctctttac
    20101 acagtgcctg cgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt
    20161 caaacggagg atagggggct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa
    20221 gactaaaacc tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc
    20281 ttagtgagag agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc
    20341 gccttgttct acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc
    20401 cacttcaact tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat
    20461 gatgctgacg atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag
    20521 cagtacctgc ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc
    20581 agaaagatgc agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt
    20641 ttctgcacgg gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat
    20701 caggttttgc tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac
    20761 gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt
    20821 gcctgcaatg ttgagcgtac cggagcgcgg catctcaagc agattgagta cacgcagcag
    20881 cgagctttta cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc
    20941 cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg
    21001 aatactcatt gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc
    21061 tttaacagaa taattatgca atatttatgc tttaaaagtt aaaagcaaag cgcattattc
    21121 aataaacata gcacaaaata acgggggcgg tggtcggtga gcataaatgt cggcattcct
    21181 cacgaaatgc cggataattt acggggttta ttggttgatc aaggcgttag cgattctcga
    21241 tggactgacg gagcgtaccc gccgtggcat gaacgctacc gcctaagtag cgcacatcgt
    21301 cgatgaccca acactggcct tcctgaatca ttaacacttc atcctgccag ccctggtcac
    21361 cctgtttgag atccacccgc aatggaatgt tacgggcatc acgattaggg atagtcgatg
    21421 cactggcaac gtgggcgctg tctggcaagg tggttcgact ggagaacgga tcgctgctta
    21481 acaattcacg atgactatta tcgcggctgg catcgctaag ttgggttgcc agtttgtcac
    21541 tcaaataggg gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcgtaga
    21601 attgttgagc cacactgtca ggccctccct ggacacagga accggtgcgg gggccgttat
    21661 ctttataagc tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta
    21721 gagatagtga acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta
    21781 cgaccaaacg ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg
    21841 aacacctgat tgggtttgag tccattgctg gcgacccact caaggttgct cgtagctgac
    21901 ttacttaaat tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata
    21961 tggctgaaat gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc
    22021 tgtagaggtt aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga
    22081 atgcggttat tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta
    22141 agatgattgc caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct
    22201 ttgactctcg ataatattat ttatgctaca attgtgggta aagaatttaa gctaatgaat
    22261 ttgagtgaag aataaattaa attgcttcag tatttcgtta attaatataa atacaaaagg
    22321 cgttgtgctt ttataattat aaagcgcgag gagttcggtg gattatcgtt tttcttgggt
    22381 gatttttaat atgataaaca atatatctga tcaagcatca tcatttccag gcacacaact
    22441 aaatcaaagt gataatttcc tggattcact gagggaattt ttcgcgatat taaatcccag
    22501 tcgtaagggt gagttgtcta catgggatac catctacttg catctgatat tagcaataaa
    22561 tgctgacagt gatctcataa aaaatgatgt gttgctcgcg gaaaatattc catcagcgaa
    22621 ttaccaattt aatacatttt ttagcaatac tttcgagatt gatgttaaaa aatatctggg
    22681 taaatcagaa gataacgaag ttgaaataaa agcgggtaat gaaagaatct ctattggaat
    22741 acgtaatgta tcgaatggaa aacttgaaag acaacagttt cttttcccac tggactatga
    22801 gaataaatta caagaacaat tagataagta ttttacgatt gaatcgcacc cattattata
    22861 caggtatacc attggtagta agattgcgaa tgtgattttt gaaaagttat acagtcgtat
    22921 tgactttaac aaagaacaat atatatcctt tataaaggac gcatttattc atttttatga
    22981 ttatagcagg cgatatgcta ttagtgagaa tatcgataaa gacgctgtaa caaacaatat
    23041 cgcattgatg agcacttttt atgacagtga taatacgtca ggtgaggttt taaataatga
    23101 ttttacagaa gaagagtcat ttgaaactgc gttagatgta gagcatgcaa tagtgcttgg
    23161 atttgctgat gataattttg aaacaaaacc tgttcattat caggatttat taacacgatt
    23221 tagtgccttt caagataccg tgtttaatct gtttcctgaa atgcactctt cgcattacca
    23281 cgatatttgt agcgtaagtg tggatatgac aaagggaacg caatgcatga ttcacttgat
    23341 ggtgaatgaa gaagttttca tgtctctgcc tgttccagtt gccacgatgg tacgtgaaga
    23401 tgcaagtaat ttagtcaatc ttaaaacact actcaatgat ggttgtttca ttaaatatag
    23461 tcattttaat gacgtggcat taataaagca aaacataagt aatctctatt tatcacacac
    23521 tgttgtgaat gaaagcatat tgaaaaaatg ttgttttgaa aacggcagtt taggtgatgt
    23581 taaaataaca aatagcaatg taataaacag tgcattcaaa aatatttcgt ttagatctgt
    23641 taaaattaat aacgtaaata ctcattcttt aaaatttatt aactgcaatt ttttcaatgt
    23701 tgatatgata agagttaatc tcagcaaatg tctttttcat gaatgtagta tgcatggtgt
    23761 caagataaaa ccctggttgc ctgtaaaatg gacaaaagaa ttaataagtg attatttgta
    23821 tggctgttta ctatctctgt actctatctg tgcaagggat atatataata tgaacgccgg
    23881 gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc ttaaaaataa
    23941 atattgtatt aaactattaa gcgctcagga tcgggcgttt atatacgagt ttgcaagaat
    24001 gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc
    24061 ggctgaccaa aaagcaatcc agcggtatag acctcagtca caagatgggg aagactttag
    24121 gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt gcaaaataat
    24181 aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac ggataatcgt
    24241 aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt
    24301 cttcccaaca tggcatatca gtccgataat gctttagaga tcgaaggctt aacaaacaag
    24361 gagatcgatc atgcaatttt ctacaacccc aactctggaa ggcctgacca tcgttgaata
    24421 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
    24481 cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc
    24541 acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt
    24601 agttggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
    24661 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc
    24721 gctctgttac tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag
    24781 cttgataccc gacaccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac
    24841 tacaccgcag atgattttga tagctcgctg gcgacactga ctgataagca ggtcagctcg
    24901 cattatctga tccctgcggt gccaccgcga tacaacggta aaccgcgcat ctggcaactg
    24961 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
    25021 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcatccgga
    25081 gtgaaatatt ttgccccgtt tgaacaggca cagattcagg cgcttattcc gctggcgaaa
    25141 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca
    25201 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
    25261 attggtgcct ggccggatgc gcagcgggtg aacttttacc ttgccgggcg cgcgccgcac
    25321 acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgtaaaa
    25381 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc
    25441 ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg
    25501 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
    25561 ttctcgatgc cttcatccag ggtgatgacc ggctgataac ctaactcttc ctgcgcacgc
    25621 gtaatatcca gcgtaaagtc aaaattcaac ttggaggcgc cgtagtgggt cagcggcggc
    25681 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg
    25741 taagggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
    25801 ctgcgcagtg tgcgatgctc gccgttggtg atgttgtaca cacgcccgga aggtagctta
    25861 tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg cattttcata gtaggtcata
    25921 tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc
    25981 gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
    26041 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
    26101 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatattgcga
    26161 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
    26221 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
    26281 tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
    26341 agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgtcggaac aaactctgcg
    26401 cccatttttt ccagcaattt acccattgct tcgttgcgac cgctcgcacg cacgctgatg
    26461 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
    26521 agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct tccgtgattt
    26581 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
    26641 tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg
    26701 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
    26761 catcgatagt acgatagtca cctttatctt ccagggtaaa acacagtcgt cccagccccg
    26821 gcgctttcat gccaaataac aacgtaagtt gctttttcgg ttcaacgaca atcactttcc
    26881 agctatccac cgcatcgccg gtctgtaaat attcgcgctc cgggcggcct ttcgccagat
    26941 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
    27001 aacgctcttt accgccgatt tggttcacca ccagccataa agccgcaagg ctggcggacg
    27061 ttttaacggt aaaccccgcc tgtttggcga aataaccgta ctccggtcgc cagcgggcaa
    27121 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcttcc tctttcaacg
    27181 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg
    27241 tatcatctgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
    27301 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
    27361 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
    27421 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gttggctgga tggtctaaca
    27481 gcgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc
    27541 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg
    27601 ctgaacctgc gccaacgata attccggccc gaagttcggt cactggtaca ttcgcttcac
    27661 gaagaatgtc cgccgtagcc tgacgagctc gcagatgatc cgactgctca tgtggcgggg
    27721 cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
    27781 cgttgagagc cagctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
    27841 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
    27901 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
    27961 gtgccgccgc taggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa
    28021 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
    28081 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc
    28141 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
    28201 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
    28261 atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct
    28321 tcctgcaacc gtgcgacgtt attttccagg gcatacatcc cggcggcagc cagaatcccg
    28381 gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca
    28441 cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
    28501 aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta
    28561 aagatgcgcg caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa ttcccatgct
    28621 tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttccag actgagtaat
    28681 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
    28741 agcgtgccgt cggcagccgc gtcgatgggt tgcggctgaa tactgcccag caccgctgcg
    28801 ccaccggctt caaacagata gttatgcgcg gcctgaccga caatatactc ttcgccacgt
    28861 tcgcagtgac tgagcagagc gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg
    28921 gcttctttac cggaaagctc tgctgcatag tcctgcagag cattaacggt agggtcgtct
    28981 ccgtaaacgt cgtccccaac cggggcggcc atcatcgctt cgagcatggc gcggctcggt
    29041 cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
    29101 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
    29161 ctgataattg cgcgcaacat atacaggcta aaacctttgg cctgttcgag tttgatctgc
    29221 ggtggaatgg ctaattcttc tttggcgacc accacatcca ccaacaccgg accgtcgata
    29281 gagaaggcgc gttgcagggc ttcatcaact tcagaggctt tttctacacg gatacccgta
    29341 atgccgcacg cttcggcaat gcgggcaaag ttggtgtcgt gcagctcagt accgtctgtc
    29401 aggtaacctc cggccttcat ctccatcgcc acaaagccca gcacgctgtt attaaagacg
    29461 ataattttca ccggcagttt catctgcact actgagagga aatcgcccat caacatgcta
    29521 aaaccgccat cgccgcacat ggcgaccacc tgacgttccg gctcggttgc ctgcgcaccc
    29581 aacgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
    29641 ttgccgttca ttttcagata acgtgccgcc cataccgttg gcgtgccgac gtcacaggta
    29701 aagatggcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
    29761 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcatcgcg gtaatcttcc
    29821 agccctttgc gggcatcgcg gtaatcttcc agcgctttat ccagaaactt gcgatcggtt
    29881 ttttcttcca ccagtggcag caatgcgcgc agggttgatt tgatatcgcc gaccagcgcc
    29941 atatctacct tgctatgcgc gccgatgctg gctggattaa tatcaatctg aataattttg
    30001 gcatcggtcg ggtagaacgc acggtagggg aactgagtac cgagtagcac taacgtgtcg
    30061 gcgttcatca tggtatggaa acctgacgag aatccgatta acccggtcat tccaacatca
    30121 tacggattat cgtattcgac atgctcttta ccgcgtaggg catgaacaat aggtgcttta
    30181 attttcccgg caaactcaac taactcttta tgcgcccccg cgcagccgct gccacacatc
    30241 agggcgatat tgctggaata acgcagcagt tgcgccagtt tgcgtaactc ttcttcttcc
    30301 ggcatcacga ctggctgtgg cgcatgatac cagtgcgtgg ttgccccttc tggcgcaggt
    30361 tttaacgcca cgtcgcccgg taatacaaca acggaaacgc cacggttaag caccgctttg
    30421 cgcatagcaa ttgccagcac ttgtgggatc tgctccgggc tggaaaccag ctcgcaatag
    30481 tgactacatt cgcggaatag ctcttgtggg tgggtttcct ggaaatagcc gctgccaatt
    30541 tcgctggagg gaatatgagc ggcaatcgcc agtaccggaa cgttattgcg gtggcaatcg
    30601 aataggccgt tgatcaagtg caggttgcct gggccgcacg atccggcaca gaccgccagt
    30661 tctcctgaaa gttgtgcttc agcgccagcg gcaaacgccg ccacttcttc atggcgggtg
    30721 gacatccact cgatggtgcc catgcgatta agactgtcgc taagaccgtt cagagagtcg
    30781 cccgtgactc cccagatgcg tttcacgcct gccgattcga gtgttttggc gatataagct
    30841 gcaaccgttt gtttcatggt tctccatctc ctgaatgtga taacggtaac aagtttagtt
    30901 catctgacgg agggggaagg gatgggagag aaaggaggca ctaacggtta aatagcccga
    30961 tgaaaggaat atcatcgggc ataaggcgat tatgcgagaa ccaaatcccc ctgcggatgg
    31021 caggagcagg ccagtacgta accttcagcg atttcggcgt cggtcagcgt cattgtactg
    31081 ctcaccgtat attcaccgga aaccactttt gtcttacagc agccgcaaac acccgcacgg
    31141 caggcagcga caaccggaac gttattgctt tccagcgcct ccagtagcgt ggtgccaacc
    31201 ggggcgtaaa attctcgtgc cggttgcagt ttggtgaatt tcagaccgct ggtcgccgct
    31261 tctgctactg gggtgaagaa tttctcttta aagaaacgcg tcacgccgag cgctttcact
    31321 tcctgctcta cccaatccat atacggagcc gggccgcagg tcatcacggt acgtgaagct
    31381 aagtcaggta catctgccag cagttcgcga gtgaggcgac cagcgataaa gccttcggta
    31441 acgttatttt ctgccaccag cgttaccgga tagttacgcc actcatcggc gaaaataaca
    31501 tcctgcggcg tacgcacgtt gtagatcacc cgcacatcgg cctgtggacg gttcttcgca
    31561 agccagcgac gcatcgacat aatcggcgtg acgccgcagc ctgccgccag caacaggaat
    31621 ttatcttctg ctttatcgtc gcaggtaaat tcccccatcg cgtccgaaag ccagagataa
    31681 tcaccgcgtt ttacatcgcg cgtcagccac tgggagccga caccgtcatc aatccgccgc
    31741 acggtcaggg tgatatattc actcacgcct ggcgtggagg aaatggtgta agcacgcagc
    31801 gtttccgctg agtttcgcac gctgaccagt gcatattgcc cggcgcgata tgggtagtga
    31861 tcgtggcaaa tcagggaaat cgtccacaca tccggcgttt cttgcgtaat gtgatgaacc
    31921 tgtatccgcc acgggcattg attcgttggc atcgtcatcg acaaactcct tacgcgttca
    31981 acagttgctt catgtcttct tcaacagtgg tgatagaacg caggccgaat ttctcgttca
    32041 gcaccgccag caggtctggt gtcaggaaac caggtgcagt cgggccggtg acgatatttt
    32101 tcacgcccag agaaagcagc gtcagcagaa tgacaatcgc tttctgttca aaccaggaga
    32161 gcaccagcga cagcggcaga tcgttcacac cacagcccag tttctctgcc agagtgacag
    32221 ccagaataat tgctgagtaa gcatcgttac attgacctgc atctaccaga cgcggcagac
    32281 cttcgatatc gccaaactca agtttgttaa agcgatattt accacaggcg agggtcagga
    32341 tcaggcagtc atccggcacg ctggtggcga aatcggtgaa gtagtggcgc tcgccgcgtg
    32401 cgccgtcaca gccaccaagc aggaagatat gacgcagttt ttcacggcta accagatcaa
    32461 tcagcgtatc ggctgcacca agcagcgtct ggcgaccaaa gcccacggtg atcaggtgag
    32521 gaatttcgct gtacgggaag cccgccattt gttgcgcctg ggcgatgacc gcagagaaat
    32581 cttcgccatc cagatgacgc acgccaggcc agccaacgat gctgcgggtc cagatacgat
    32641 cgtcataagc gcctacggtt gggtcgatga tgcagttcga ggtcatcacg atggggccag
    32701 ggaaacgagc gaactccact tgctgattct gccagccgct gccgtagtta ccgaccagat
    32761 gcttgaattt acgcagctcc gggtagccat gcgcaggcag catttcgccg tgggtgtaga
    32821 cattaacgcc cgtgccttcg gtctgttcca gcaggttgta gagatctttg agatcgtgac
    32881 cggaaatcag aatgcattta cccgccgtcg ctttgacgtt gacctgggtt ggcgtcgggt
    32941 gaccgtattt accggtttcg cctgcatcca gaatgctcat cactttgaag ttcatctggc
    33001 cgatttccat tgaacactca agaagcgcgt tcatatcggc aggccaggtc cccagccacg
    33061 ccatgatttt atggtactgg gcataaatat cgttgtcgta ttgaccgaga acatgcgcgt
    33121 gttccatata ggccgccgca cctttcaggc catacaggca cagcagacgc aggccgagaa
    33181 tgttttcgcc aatcgccgct ttatctttgt taggggtaaa ttctgctgcc tgacgttgca
    33241 gctcgccgag atcatcgctc accagttgca ggtcagccat cgggttatcg acgcgcgcgt
    33301 tggcatctac agccaggcat tgtgctttca gcgcctcgcg caaggcaatc gcttcacgag
    33361 cgtagccgac aatacgcgga gaatcgaagt taacgttggt cagggttgag aaaaaggcac
    33421 gtggcgcgaa gctgtcaaca tcgtggttga tgatgccgta ttcacgcgct tttaccgccc
    33481 aggcagaaag cccttgcagc gccgcgatga gtaaatcctg aaggtcagaa gtttccgccg
    33541 ttttaccaca catcccctgc gcgtatgagc agccgttttc tgccggagta cggatagttt
    33601 gttcacattg cacacaaaac atgatcacac cttttaaagt tatatttaat atacatgttt
    33661 aagggtaaga cgcttaacgc ggggataaaa gggatttttc atgcaacttt aagggagatt
    33721 gatttagcgc aattttggcg gcagggaact accgccagag aggtattacg cagagaaaaa
    33781 ggcgatgagg atcggcacta acaggctaag aataaaaccg tgaacaattg ccgccgggac
    33841 catatccagc ccgccagtac gttgaagaac gggcagggtg aaatccattg atgtggcacc
    33901 gcataagccc agtgcagtag agcggctgcg gcgaatcagc ccagggatca
//
LOCUS       DAJTRN010000038        33147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRN010000038 DAJTRN010000000
VERSION     DAJTRN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(2..94)
                     /locus_tag="OO328_001853"
     tRNA            complement(2..94)
                     /locus_tag="OO328_001853"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO328_001854"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO328_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5930442.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO328_001855"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO328_001855"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5930443.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO328_001856"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO328_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5930444.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO328_001857"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO328_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5930445.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO328_001858"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO328_001858"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5930446.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO328_001859"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO328_001859"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5930447.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO328_001860"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO328_001860"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO328_001861"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO328_001861"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5930448.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO328_001862"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO328_001862"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5930449.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO328_001863"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO328_001863"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5930450.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO328_001864"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO328_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5930451.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO328_001865"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO328_001865"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5930452.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO328_001866"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO328_001866"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5930453.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO328_001867"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO328_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5930454.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO328_001868"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO328_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5930455.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO328_001869"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO328_001869"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5930456.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO328_001870"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO328_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5930457.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO328_001871"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO328_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5930458.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO328_001872"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO328_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5930459.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO328_001873"
     CDS             complement(20900..21019)
                     /locus_tag="OO328_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5930460.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO328_001874"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO328_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5930461.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO328_001875"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO328_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5930462.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO328_001876"
     CDS             complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO328_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5930463.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKS
                     VLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIY
                     QSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATC
                     APLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACP
                     LLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKW
                     LNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO328_001877"
     CDS             complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO328_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5930464.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO328_001878"
     CDS             complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO328_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5930465.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26945..27295
                     /gene="hypA"
                     /locus_tag="OO328_001879"
     CDS             26945..27295
                     /gene="hypA"
                     /locus_tag="OO328_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5930466.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27299..28171
                     /gene="hypB"
                     /locus_tag="OO328_001880"
     CDS             27299..28171
                     /gene="hypB"
                     /locus_tag="OO328_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5930467.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28162..28434
                     /gene="hypC"
                     /locus_tag="OO328_001881"
     CDS             28162..28434
                     /gene="hypC"
                     /locus_tag="OO328_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5930468.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28434..29555
                     /gene="hypD"
                     /locus_tag="OO328_001882"
     CDS             28434..29555
                     /gene="hypD"
                     /locus_tag="OO328_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5930469.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29552..30562
                     /gene="hypE"
                     /locus_tag="OO328_001883"
     CDS             29552..30562
                     /gene="hypE"
                     /locus_tag="OO328_001883"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5930470.1"
                     /translation="MNNIQLAHGSGSQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30636..32714
                     /gene="flhA"
                     /locus_tag="OO328_001884"
     CDS             30636..32714
                     /gene="flhA"
                     /locus_tag="OO328_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5930471.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32751..33104)
                     /locus_tag="OO328_001885"
     CDS             complement(32751..33104)
                     /locus_tag="OO328_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5930472.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7810 a         9241 c         8626 g         7470 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagcaggat aatgccgata
    24661 ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gttccatcaa cgcatacaga
    24721 ccaccgacga acgcggtgat catgccgagc accagcagcg cgatccccca ccacagcggt
    24781 gcattaccgc ccagcagtga cagggttaac atgcccagca ggccaatttt catgactacc
    24841 gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg cacccagccg
    24901 tgcagcggaa taatcccagc cagcaggcca aagccaatca caccgagcag ccagatatcg
    24961 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccataa
    25021 cgctgccaca gcagccagca agcaatcacc agcagcagag tgccaagacg cctcagcgca
    25081 aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacaa ggccatgact
    25141 tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac ggcggcagcc
    25201 atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg
    25261 ttatagaggc tgacaaacag accgcaaagg ccgagggtaa tcagccaaat cgcgttaagc
    25321 ggggagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca
    25381 gtcagtacag tgaagcccgc ggctgccgta tacagactac caaccgcgcc gccaattccg
    25441 gctatccagc cacttagcgc tttttgaaaa gagaaatgcc agaacagcgg cggcgacaaa
    25501 ccatgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    25561 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    25621 ctgacgcggg cgatatcggt gttatccacc agatgcaggg ctttagtcgg gcacatccgc
    25681 acacaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    25741 atacctggca cccagtcaag caatgtgctg acacgcgccg gagcaggcgg tgccggtggc
    25801 gctttcgggg tattggcgtt tgccggaata tccagcggac ggctgccgga aaattcaatt
    25861 gcgccaaacg ggcaggcgat gccgcacagt ttgcagctta cgcacagact ttcattcaac
    25921 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    25981 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattgagcat cactctcagg
    26041 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaggtg
    26101 tggcagccga tacagagcgt ggagtcggca attacaaaac gattcaccag gcattcctca
    26161 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    26221 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    26281 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    26341 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    26401 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    26461 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    26521 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    26581 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    26641 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    26701 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttactcgttt aacagagatg
    26761 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    26821 ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    26881 cgcagggata atcaacactg gcgcaattat tgcttgtagc tggcaatagt taatgggagg
    26941 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    27001 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    27061 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    27121 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    27181 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    27241 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    27301 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    27361 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgcaga tgaaaatcac
    27421 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    27481 tcatggcgaa gcgggcactc acacaccggg catgagccag cgtcggatgc tggaagtcga
    27541 aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    27601 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    27661 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcggttattg aaggcgacca
    27721 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggt acaccagcga ttcaggtgaa
    27781 caccggtaaa ggctgccatc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    27841 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    27901 cttcgatctc ggtgaaaaac acaaagtagc ggtgctttcc gttaccgaag gtgaagacaa
    27961 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    28021 cctgttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa
    28081 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    28141 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    28201 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    28261 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    28321 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    28381 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    28441 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    28501 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    28561 gtcataccca cgccattttt aaattcggcc tcgaccagtt actgcctgaa aacgttgagt
    28621 ttatccacgg tccgggttgc ccggtgtgcg tactgccgat gggtagaatc gacacctgcg
    28681 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    28741 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    28801 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgcaaa gtggtgttct
    28861 tcggcttagg ttttgaaacc accatgccga ccaccgccat cactctgcaa caggcgaaag
    28921 cgcgtgatgt gcagaatttt tacttcttct gccagcacat tacgcttatc ccgacgctgc
    28981 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    29041 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    29101 tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga
    29161 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg ggtggtaccg gatgccggta
    29221 acctactggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    29281 gcggcttagg cgtgattgaa tcttctggcg tgcacctgac gccggattat caacgattcg
    29341 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt
    29401 gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    29461 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    29521 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    29581 cggcagccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    29641 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcggaagg
    29701 cgaccgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    29761 taatatcggc aagctggcga tttgcggcac cgcgaatgac gttgcgggca gtggcgctat
    29821 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    29881 agccgtagtg accagcatgg cagaaaccgc ccgcacggca ggcattgcca tcgttactgg
    29941 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    30001 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    30061 gctggttagc ggtacactcg gcgaccacgg ggcgactatc cttaacctgc gtgagcagct
    30121 ggggctggat ggcgaactgg tcagcgactg cgcggtgctg acgccgctta ttcagacgct
    30181 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt
    30241 ggttcatgag ttcgcggcag cctgcggttg tggtattgaa atttctgaat cagcgctgcc
    30301 ggttaaacct gccgtgcgcg gcgtttgcga attgctggga ctggacgccc tgaactttgc
    30361 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    30421 gttacattcc catccactgg ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    30481 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    30541 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    30601 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    30661 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    30721 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    30781 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcgtcgcgt gaaaaagaca
    30841 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    30901 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    30961 ccgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg gcggcgggag
    31021 ggcatatttt tggtggctgt gaatttattc gttatgacga gcgtccctgg agcgaaaaag
    31081 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    31141 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    31201 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    31261 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagcatt gtcttacgca
    31321 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    31381 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    31441 aagagatgct gctgatcaat ctctacgagc gggacgattt agccccctat gaacgcatgt
    31501 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    31561 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    31621 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    31681 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    31741 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    31801 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    31861 gtgaaactgg ctcgggtaaa gagctgattg cccgtgcgat ccataatctc agtgggcgta
    31921 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    31981 atctgtttgg tcatgagcgt ggggctttta ccggtgccaa cgcccagcgc attggtcgtt
    32041 ttgaactggc ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt
    32101 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    32161 aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg
    32221 tcgccgaccg agagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    32281 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    32341 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct
    32401 tgagcaacat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcag
    32461 tattgctaac acgcggcaac gtgctgcagc tgtcattgcc agatattgct ttaccggagc
    32521 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    32581 ttgtgcgcgt gctgaaagaa accaacagcg tggttgccgg gcctaaaggc gctgcgcaac
    32641 gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga attgataaat
    32701 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    32761 ggttttttcg gacgatattt ttccggcagt tctggcaccg gacgcttgtc atcgatgaga
    32821 tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca acgcataatc
    32881 tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct
    32941 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    33001 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    33061 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccacctcat
    33121 taagatgcat ttatattaca tcttaat
//
LOCUS       DAJTRN010000039        32976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRN010000039 DAJTRN010000000
VERSION     DAJTRN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1119
                     /locus_tag="OO328_001886"
     CDS             <1..1119
                     /locus_tag="OO328_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3763 domain-containing protein"
                     /protein_id="HCR5930473.1"
                     /translation="LLTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDR
                     MLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHV
                     FELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWY
                     DAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTLIRLGGI
                     FSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLN
                     GIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQ
                     HALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1113..2564
                     /gene="viaA"
                     /locus_tag="OO328_001887"
     CDS             1113..2564
                     /gene="viaA"
                     /locus_tag="OO328_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5930474.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2569..3561)
                     /gene="asnA"
                     /locus_tag="OO328_001888"
     CDS             complement(2569..3561)
                     /gene="asnA"
                     /locus_tag="OO328_001888"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5930475.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            3713..4171
                     /gene="asnC"
                     /locus_tag="OO328_001889"
     CDS             3713..4171
                     /gene="asnC"
                     /locus_tag="OO328_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5930476.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4261..4704
                     /gene="mioC"
                     /locus_tag="OO328_001890"
     CDS             4261..4704
                     /gene="mioC"
                     /locus_tag="OO328_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5930477.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5083..6972
                     /gene="mnmG"
                     /locus_tag="OO328_001891"
     CDS             5083..6972
                     /gene="mnmG"
                     /locus_tag="OO328_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5930478.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7036..7659
                     /gene="rsmG"
                     /locus_tag="OO328_001892"
     CDS             7036..7659
                     /gene="rsmG"
                     /locus_tag="OO328_001892"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5930479.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8276..8656
                     /gene="atpI"
                     /locus_tag="OO328_001893"
     CDS             8276..8656
                     /gene="atpI"
                     /locus_tag="OO328_001893"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5930480.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            8665..9480
                     /gene="atpB"
                     /locus_tag="OO328_001894"
     CDS             8665..9480
                     /gene="atpB"
                     /locus_tag="OO328_001894"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5930481.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9527..9766
                     /gene="atpE"
                     /locus_tag="OO328_001895"
     CDS             9527..9766
                     /gene="atpE"
                     /locus_tag="OO328_001895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5930482.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            9828..10298
                     /gene="atpF"
                     /locus_tag="OO328_001896"
     CDS             9828..10298
                     /gene="atpF"
                     /locus_tag="OO328_001896"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5930483.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10313..10846
                     /gene="atpH"
                     /locus_tag="OO328_001897"
     CDS             10313..10846
                     /gene="atpH"
                     /locus_tag="OO328_001897"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5930484.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            10859..12400
                     /gene="atpA"
                     /locus_tag="OO328_001898"
     CDS             10859..12400
                     /gene="atpA"
                     /locus_tag="OO328_001898"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5930485.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12451..13314
                     /gene="atpG"
                     /locus_tag="OO328_001899"
     CDS             12451..13314
                     /gene="atpG"
                     /locus_tag="OO328_001899"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5930486.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13341..14723
                     /gene="atpD"
                     /locus_tag="OO328_001900"
     CDS             13341..14723
                     /gene="atpD"
                     /locus_tag="OO328_001900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5930487.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            14744..15163
                     /gene="atpC"
                     /locus_tag="OO328_001901"
     CDS             14744..15163
                     /gene="atpC"
                     /locus_tag="OO328_001901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5930488.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15514..16884
                     /gene="glmU"
                     /locus_tag="OO328_001902"
     CDS             15514..16884
                     /gene="glmU"
                     /locus_tag="OO328_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5930489.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17046..18875
                     /gene="glmS"
                     /locus_tag="OO328_001903"
     CDS             17046..18875
                     /gene="glmS"
                     /locus_tag="OO328_001903"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5930490.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19037..19858
                     /locus_tag="OO328_001904"
     CDS             19037..19858
                     /locus_tag="OO328_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5930491.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            19845..21953
                     /locus_tag="OO328_001905"
     CDS             19845..21953
                     /locus_tag="OO328_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5930492.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            21950..23617
                     /locus_tag="OO328_001906"
     CDS             21950..23617
                     /locus_tag="OO328_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5930493.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            23620..25146
                     /locus_tag="OO328_001907"
     CDS             23620..25146
                     /locus_tag="OO328_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5930494.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25147..26763
                     /locus_tag="OO328_001908"
     CDS             25147..26763
                     /locus_tag="OO328_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5930495.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(26819..26971)
                     /locus_tag="OO328_001909"
                     /pseudo
     CDS             complement(26819..26971)
                     /locus_tag="OO328_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26994..27371)
                     /locus_tag="OO328_001910"
     CDS             complement(26994..27371)
                     /locus_tag="OO328_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930496.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(27781..28152)
                     /locus_tag="OO328_001911"
     CDS             complement(27781..28152)
                     /locus_tag="OO328_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930497.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28213..28710)
                     /locus_tag="OO328_001912"
     CDS             complement(28213..28710)
                     /locus_tag="OO328_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930498.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(28786..29574)
                     /gene="aadA1"
                     /locus_tag="OO328_001913"
     CDS             complement(28786..29574)
                     /gene="aadA1"
                     /locus_tag="OO328_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5930499.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(29632..30156)
                     /gene="sat2"
                     /locus_tag="OO328_001914"
     CDS             complement(29632..30156)
                     /gene="sat2"
                     /locus_tag="OO328_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5930500.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30251..30724)
                     /locus_tag="OO328_001915"
     CDS             complement(30251..30724)
                     /locus_tag="OO328_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5930501.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31056..31592
                     /locus_tag="OO328_001916"
     CDS             31056..31592
                     /locus_tag="OO328_001916"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5930502.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            31707..32033
                     /locus_tag="OO328_001917"
     CDS             31707..32033
                     /locus_tag="OO328_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5930503.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32221..32460)
                     /locus_tag="OO328_001918"
     CDS             complement(32221..32460)
                     /locus_tag="OO328_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5930504.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8559 a         7686 c         8338 g         8393 t
ORIGIN      
        1 ttgctcaccg ccattaacga gcgccagttc cgcaacggtg cacacgtaga aaaaatcccg
       61 atgcgtctac tggtggcggc ctccaacgag ctgccggaag cagacagcag tctggaagcg
      121 ttatatgacc gcatgctgat tcgtctatgg ttagataaag tgcaagataa agcgaatttc
      181 cgctccatgc tgaccagtca acaggatgaa aacgacaatc cggttcctga tgccttgcag
      241 gtcacagatg aagaatatga acgctggcag aaagagattg gtgaaattac gctgcccgat
      301 catgtatttg agctgatttt tattctgcgc cagcaactgg ataaattacc ggatgcgcct
      361 tatgtctcgg atcgtcgctg gaaaaaagcg atccgcttat tgcaggccag cgcctttttt
      421 agcggtcgca gtgctgttgc cccggttgat ctcattttgc tgaaagattg cctgtggtat
      481 gacgcgcaaa gcctgaattt gatacaacaa caaattgatg tattgatgac cggtcatgcc
      541 tggcaacagc aagggatgtt gacccgcctg ggcgcgattg tgcaacgtca cctgcaactc
      601 cagcagcaac aaagcgataa aacagcctta acgttaattc gtctgggcgg cattttcagc
      661 cgtcgtcagc agtatcaact tcctgttaac gttactgctt ccactctgac tctgctgctg
      721 caaaaaccgt taaaactgca tgatatggaa gtggttcata tctcctttga gcgtagcgcg
      781 ctggaacagt ggctgagtaa aggtggtgaa attcgcggca aactgaacgg tatcggcttt
      841 gcccagaaac tgaatctgga agttgatagc acccaacatc ttgttgtacg cgatgtaagt
      901 ttacaaggca gtacgctggc acttcccggt tcatcggctg aaggtctgcc aggtgaaata
      961 aaacaacaac tggaagagct tgaaagcgac tggcgtaagc aacacgcttt attcagcgaa
     1021 cagcaaaaat gtctgtttat ccctggcgac tggttaggtc gcattgaaga cagcctacag
     1081 gatgtcggtg cacagattcg tcaggcacaa caatgctaac gctggatacg cttaatgtga
     1141 tgctggccgt cagcgaagag ggattgatcg aagagatgat catcgcactg ctggcctcac
     1201 cgcagctggc ggtcttcttt gaaaaattcc cacgactgaa ggcggcaatc actgatgatg
     1261 ttccccgctg gcgtgaggcg ctgcgcagtc ggctgaaaga tgcccgagtc ccgccggaac
     1321 tcaccgaaga ggtgatgtgc tatcagcaaa gccagctcct ctccacgccg cagtttattg
     1381 tgcagctacc acagatcctg gacttactgc atcgtctgaa ttccccatgg gcagaacaag
     1441 cccggcagtt ggttgatgct aacagcacaa taacttcagc gttacacacg ctttttctcc
     1501 agcgttggcg tttaagtctg atcgtgcaag caacgacgtt aaatcaacag ctattagaag
     1561 aagaacgcga acaactgtta agtgaagttc aggaacgcat gacgctgagc ggacaacttg
     1621 aaccgattct cgcagataac aataccgcag ctggtcgtct gtgggatatg agcgccggtc
     1681 aacttaaacg tggcgactat cagtttattg tgaaatacgg tgaatttctt aacgaacagc
     1741 cggaactgaa acgcctggca gagcagctgg ggcgttctcg ggaagccaaa tcaataccgc
     1801 gcaacgatgc gcagatggaa accttccgca ccatggtgcg cgaaccggcg acggttcctg
     1861 agcaggttga tggtctgcaa caaagcgatg atattttacg tctcctgccg ccagaactgg
     1921 cgacactagg gataacggaa ctggagtatg agttttaccg tcggctggtg gaaaaacagt
     1981 tgctcaccta tcgcctgcac ggtgagtcgt ggcgtgaaaa agtgttcgaa cgtccggtgg
     2041 tacataaaga ttacgatgaa cagccgcgcg ggccgtttat tgtctgtgtg gatacttccg
     2101 gctcaatggg cggctttaat gaacagtgtg cgaaagcgtt ctgcctggct ttgatgcgca
     2161 ttgctctcgc tgaaaaccgg cgctgttata ttatgctatt ttccaccgag atcgtccgtt
     2221 atgagctttc aggcccacaa ggcatcgaac aagcaatccg ttttttaagc cagcagtttc
     2281 gtggcggcac cgatcttgcc agttgttttc gcgccattat ggaacgcttg caaagcaggg
     2341 aatggtttga tgccgatgcg gtggtgattt ctgattttat cgctcagcgg ttgcctgacg
     2401 acgtgacgag taaagtgaaa gagctgcagc gggtacatca gcatcgcttt catgccgtgg
     2461 cgatgtcggc acacggcaaa cccggcatca tgcgcatttt cgatcatatc tggcgctttg
     2521 ataccgggat gcgaagccgc ctgctcagac gctggcggcg ataaattatt acagcagaga
     2581 agggacgctc tcgcgaacag cagctggcca tactccacac tgaacctggc cgatatgcgg
     2641 cagttgcagc agcagcatag tcaaacgaga ctggccgata ccgccgccga tggtctgcgg
     2701 catttcaccg cgcagcagcg cctgatgcca ttccagctgc agacgatctt catcaccggt
     2761 cagcgccagc tgatgcttca gcgtgtcggc atccacgcgg atccccatgg aagaaagctc
     2821 aaacgcatct tccagtaccg ggttccacac cagaatatcg ccgttcaggc ccgcatggcc
     2881 cagctctgac ggggtgctcc agtcatcata atccggtgcg cgcacgtcgt gacgatgacc
     2941 atcactcaac ttgccgccaa tcccgacgag gaataccgcg ccaagatctt tcgcaatcgc
     3001 ccgctcacgc cctttggcat cgagatccgg ataacgagac agtaactcct ggctgtgtac
     3061 gaagtgaatc tgatccggca ggaacggtgc caggccaaac tcttcgttaa ccgcagcttc
     3121 ggttgcttta attcccgccc agatcgcctc taccgtgctt ttcagagtcg agagttgacg
     3181 ctcaccgtcg cccattacgc gttcccagtc ccactggtca acatagaccg agtgcaacgg
     3241 agaaagacgg tcttcatcgg ggcgaagggc tttcatgtgc gtgtacagcc cttcgcccgc
     3301 gctgaagtcg tgttgcccta aggtctgacg tttccacttc gccagtgaat gaaccacttc
     3361 gaactgggca tcaggcagag ctttcacttt tacctgcacc gctttttcac agcccgacaa
     3421 gttatcctgc gtgccatccc ccacacggct aagaatcggc gcctggactt cgatcagccc
     3481 cagacgttct tccagttggc gagaaaagtg agatttcacg aagctaattt ggcgttgttt
     3541 ggcaatgtaa gcggttttca ttttttatac tcctgcgtcc tgttgcttat gattaagcaa
     3601 caaaaaccga cacacatgca ataatcattc aataaaaagc ctaacacact tttgattcat
     3661 taaaaaagac ggctaaaata gaatgaatca tcaatctgca taagaaaatc ctatggaaaa
     3721 ttatctgatc gacaatctgg accgtggcat cctggaagca ttaatgggca atgcgcgcac
     3781 cgcttacgcc gaactggcga aacaatttgg cgtcagtccg gggacgattc acgttcgagt
     3841 agagaaaatg aagcaggcgg ggatcattac cggggcgcgt attgatgtca gcccgaagca
     3901 gctcggttat gacgtaggct gctttatcgg cattatatta aagagcgcca aagactaccc
     3961 ttccgcgctg gcaaagctgg aaagccttga tgaagtcact gaagcctatt acacaaccgg
     4021 ccactacagc atctttataa aagttatgtg ccgttcgatc gacgctctcc agcatgtact
     4081 tatcaacaag atccaaacaa ttgatgaaat tcagtccacc gagacattga tcgtcctgca
     4141 aaacccgatc atgcgtacca tcaagccctg atcggctttt ttaatcccat acttttccac
     4201 aggtagatcc caacgcgttc acagcgtaca atacgccact cttaataaag gtggcggttt
     4261 atggcagata tcactcttat cagcggcagc accctcggcg gtgccgaata tgtagcagaa
     4321 cacctggctg aaaagctgga agaggtgggt tttaccaccg aaacgctgca cggtccgctg
     4381 ttggaagatt tatctgcctc ggggatctgg ctggttatca gctccaccca cggtgccgga
     4441 gatattccgg acaacctttc tcctttctat gaagcattgc aggaacagaa gcccgatctt
     4501 tctgcggtcc gctttggcac aatcggtatt ggcagtcgtg aatatgacac cttttgtggg
     4561 gctatcgata aactcgaggc agaactcaaa aattccggtg caaaacagac aggcgaaaca
     4621 ctgaagatca acattcttga tcacgacatt ccggaagatc cggcagaaga atggctggga
     4681 tcgtggatta atttactcaa ataagtatac agatcgtgcg atctactgtg gataactctg
     4741 tcaggaagct tggatcaacc ggtagctatc caaagaacaa ccgttgttca gtttttgagt
     4801 tgtgtataac ccctcattct gatcccagct tatacggtcc aggatcaccg atcattcaca
     4861 gttaatgatc ctttccaggt tgttgatctt aaaaatcgga tccttgttat ccacagggca
     4921 gtgcgatcct aataagagat cacaatagaa cagatctcta aataaataga tcttcttttt
     4981 aatacccagg atcccaggtc tttctcaagc cgacaaagtt gagtagaatc cacggcccgg
     5041 gcttcaatcc attttcatac cgcgttatgc gaggcaatca ccatgtttta tccggatcct
     5101 tttgacgtca tcatcattgg cgggggtcat gcaggcaccg aggccgcgat ggctgcggcg
     5161 cgtatgggtc aacagactct gcttttgaca cacaatatcg acactctggg gcagatgagc
     5221 tgcaacccgg cgatcggcgg tattgggaag ggacatctgg taaaagaagt ggatgcactc
     5281 ggcggtctga tggcgaaagc gatcgatcaa gcgggtatcc agtttaggat actaaacgca
     5341 agcaaaggac cggcggttcg cgctacccga gctcaggcgg atcgtgtgcg ctaccgtcag
     5401 gcggtacgta cggcgctgga gaaccaaccg aacctgatga tcttccagca ggcggttgaa
     5461 gatcttattg tcgaaaacga tcgcgtggtc ggtgctgtta cccaaatggg actgaagttc
     5521 cgtgccaaag ctgtggtgct caccgttggg acgttcctcg acggcaaaat tcatatcggt
     5581 ctggataatt acagcggtgg ccgtgctggc gatccgccat ccattccgct ctctcgccgt
     5641 ttgcgtgaac tgccgctgcg cgttggtcgt ctgaaaaccg gtactccgcc gcgtattgat
     5701 gctcgaacca tcgactttag cgtgctggcg caacagcatg gcgataaccc aatgccggta
     5761 ttctcgttta tgggcaatgc gtccctgcat ccccagcagg tgccgtgtta tatcactcat
     5821 accaacgaga aaacccatga tgtgatccgc agtaacctcg atcgtagccc aatgtatgca
     5881 ggggtgatcg aaggtgtcgg cccacgctac tgcccgtcga tcgaagacaa agtcatgcgc
     5941 ttcgcagaca gaaatcagca tcagatcttc cttgaaccgg aaggactgac ctctaatgaa
     6001 atttatccga acggtatctc caccagcctg ccgttcgatg tgcagatgca aatcgtccgc
     6061 tctatgcagg ggatggaaaa cgcgaagatc gtgcgtccgg gttatgccat tgagtatgac
     6121 ttcttcgatc ctcgcgacct gaaaccgacg ctggagagca agtttatcca ggggctgttc
     6181 tttgctggtc agattaacgg cactaccggt tacgaagaag ccgctgcgca aggtttgctg
     6241 gccggtctta acgctgcccg tctgtctgct gacaaagaag gttgggctcc ggcacgttct
     6301 caggcgtatc tcggcgtact ggttgatgac ctgtgcactt taggaaccaa agaaccgtat
     6361 cgtatgttta cttcgcgcgc agaatatcgt ctgatgctgc gcgaagataa tgcggatctg
     6421 cgtttgactg aaatcggtcg tgaactgggc ctggtggatg acgaacgttg ggcgcgcttt
     6481 aacgagaaac ttgagaatat cgagcgtgag cgtcagcgtc tgaaatcgac ctgggtaacc
     6541 ccgtcggcgg aagctgcagc cgaagtgaat gctcacctga ctgcgccgct ttcccgtgaa
     6601 gccagtggtg aagatctgct gcgtcgtccg gaaatgactt atgaaaaatt aaccacgctg
     6661 acgccgtttg cccctgcgtt gacagacgaa caggcggcgg aacaggttga gattcaggtt
     6721 aaatacgaag gttatatcgc gcgccagcaa gatgagatcg aaaagcagct gcgtaacgag
     6781 aacaccctgc tacccgcgac actggattac cgccagatat ccggtctttc taacgaagtg
     6841 atcgccaaac ttaacgatca caaaccggcc tctatcggcc aggcttcgcg tatttctggc
     6901 gtcacgcctg cggccatctc cattctgctg gtgtggctga aaaaacaggg tatgctgcgt
     6961 cgtagcgcat aacgcattaa aaatgcctgg taagcacccg cttaccaggc aacgcatcaa
     7021 gaacaggtaa tcaccgtgct caacaaactc tccttactgc tgaaagacgc aggtatttcg
     7081 cttactgatc accagaaaaa ccagcttatt gcctacgtga atatgctgca taaatggaac
     7141 aaagcgtaca acctgacttc ggtccgcgat cctaatgaga tgctggtacg ccatattctc
     7201 gatagcattg tggtggcacc gtatctgcaa ggtgaacggt ttatcgatgt cggcaccgga
     7261 ccaggactgc caggcattcc actctctatc gtgcgtcctg aagcccattt cactctgttg
     7321 gatagccttg gtaaacgcgt gcgtttcctt cgtcaggtgc aacatgagct taaactggag
     7381 aatattgaac cagtacagag cagggtagaa gagtttcctt cagagccgcc atttgatggc
     7441 gtaattagcc gcgcttttgc ctctctgaac gatatggtga gctggtgcca ccatcttcct
     7501 ggtgagcaag gccgtttcta cgcgctgaaa gggcaaatgc cggaagatga aatcgctttg
     7561 ttgcccgaag aatatcaggt cgaatcagtg gttaaacttc aggttccagc cctggatggc
     7621 gaacgtcatc tggtggtgat taaagcaaat aaaatttaat ttttatcaaa aaaatcataa
     7681 aaaattgacc ggttagactg ttaacaacaa ccaggttttc tactgatata actggttaca
     7741 tttaacgcca cgttcactct tttgcatcaa caagataacg tggctttttt tggtaagcag
     7801 aaaataagtc attagtgaaa atatcagtct gctaaaaatc ggcgctaaga accatcattg
     7861 gctgttaaaa cattattaaa aatgtcaatg agtggttttt gttgtgtaaa tgttatttat
     7921 taaaacagta tctgttttta gactgaaata tcataaactt gtaaaggcat catttgccaa
     7981 gtaaataaat atgctgtgcg cgaacatgcg caatatgtga tctgaagcac gctttatcac
     8041 cagtgtttac gcgttattta cagtttttca tgatcgaaca ggggtagcgg aaaagtcgca
     8101 attgtatgca ctggaaaaat atttaaacat ttattcacct tttggctact tattgtttga
     8161 aatcacgggg gcgcaccgta taatttgacc gctttttgat gcttgactct aagccttaaa
     8221 gaaagtttta tacgacacgc ggcatacctc gaagggagca ggagtgaaaa acgtgatgtc
     8281 tgtgtcgctc gtgagtcgaa acgttgctcg gaagcttctg ctcgttcagt tactggtggt
     8341 gatagcaagt ggattgctgt tcagcctcaa agaccccttc tggggcgtct ctgcaataag
     8401 cgggggcctg gcagtctttc tgcctaacgt tttgtttatg atatttgcct ggcgtcacca
     8461 ggcgcataca ccagcgaaag gccgggtggc ttggacattc gcatttggcg aagctttcaa
     8521 agttctggcg atgttggtgt tactggtggt ggcgttggcg gttttaaagg cggtattctt
     8581 gccgctgatc gttacgtggg ttttggtgct ggtggttcag atactggcac cggctgtaat
     8641 taacaacaaa gggtaaaagg catcatggct tcagaaaata tgacgccgca ggattacata
     8701 ggacaccacc tgaataacct tcagctggac ctgcgtacat tctcgctggt ggatccacaa
     8761 aaccccccag ccaccttctg gacaatcaat attgactcca tgttcttctc ggtggtgctg
     8821 ggtctgttgt tcctggtttt attccgtagc gtagccaaaa aggcgaccag cggtgtgcca
     8881 ggtaagtttc agaccgcgat tgagctggtg atcggctttg ttaatggtag cgtgaaagac
     8941 atgtaccatg gcaaaagcaa gctgattgct ccgctggccc tgacgatctt cgtctgggta
     9001 ttcctgatga atctgatgga tttactgcct atcgacttgc tgccgtacat tgctgaacat
     9061 gtactgggtc tgcctgcact gcgtgtggtt ccgtctgcgg acgtgaacgt aacgctgtct
     9121 atggcactgg gcgtatttat cctgattctg ttctacaaca tcaaaatgaa aggcatcggc
     9181 ggcttcacga aagagttgac gctgcagccg ttcaatcact gggcgttcat tcctgtcaac
     9241 ttaatccttg aaggggtaag cctgctgtcc aaaccagttt cactcggttt gcgactgttc
     9301 ggtaacatgt atgccggtga gctgattttc attctgattg ctggtctgtt gccgtggtgg
     9361 tcacagtgga tcctgaatgt gccgtgggcc attttccaca tcctgatcat tacgctgcaa
     9421 gccttcatct tcatggttct gacgatcgtc tatctgtcga tggcgtctga agaacattaa
     9481 tttaccaaca ctactacgtt ttaactgaaa caaactggag actgtcatgg aaaacctgaa
     9541 tatggatctg ctgtacatgg ctgccgctgt gatgatgggt ctggcggcaa tcggtgctgc
     9601 gatcggtatc ggcatcctcg ggggtaaatt cctggaaggc gcagcgcgtc aacctgatct
     9661 gattcctctg ctgcgtactc agttctttat cgttatgggt ctggtggatg ctatcccgat
     9721 gatcgctgta ggtctgggtc tgtacgtgat gttcgctgtc gcgtagtaag cgttgctttt
     9781 atttaaagag caatatcaga acgttaacta aatagaggca ttgtgctgtg aatcttaacg
     9841 caacaatcct cggccaggcc atcgcgtttg tcctgttcgt tctgttctgc atgaagtacg
     9901 tatggccgcc attaatggca gccatcgaaa aacgtcaaaa agaaattgct gacggccttg
     9961 cttccgcaga acgagcacat aaggaccttg accttgcaaa ggccagcgcg accgaccagc
    10021 tgaaaaaagc gaaagcggaa gcccaggtaa tcatcgagca ggcgaacaaa cgccgctcgc
    10081 agattctgga cgaagcgaaa gctgaggcag aacaggaacg tactaaaatc gtggcccagg
    10141 cgcaggcaga aattgaagcc gagcgtaaac gtgcccgtga agagctgcgt aagcaagttg
    10201 ctatcctggc tgttgctggc gccgagaaga tcatcgaacg ttccgtggat gaagctgcta
    10261 acagcgacat cgtggataaa cttgtcgctg aactgtaagg agggaggggc tgatgtctga
    10321 atttattacg gtagctcgcc cctacgccaa agcagctttt gactttgccg tcgaacacca
    10381 aagtgtagaa cgctggcagg acatgctgac gtttgccgcc gaggtaacca aaaacgaaca
    10441 aatggcagag cttctctctg gcgcgcttgc gccagaaacg ctcgccgagt cgtttatcgc
    10501 agtttgtggt gagcaactgg acgaaaacgg tcagaacctg attcgggtaa tggctgaaaa
    10561 tggtcgtctt aacgcgctcc cggatgttct ggagcagttt attcaccttc gtgccgtgag
    10621 tgaggctacc gctgaggtag acgtcatttc cgctgccgca ctgagtgaac aacagctcgc
    10681 gaaaatttct gctgcgatgg aaaaacgtct gtcacgcaaa gttaagctga attgcaaaat
    10741 cgataagtct gtaatggctg gcgttatcat ccgatcgggt gatatggtca ttgatggcag
    10801 cgtacgcggt cgtcttgagc gccttgcaga cgtcttgcag tcttaagggg actggagcat
    10861 gcaactgaat tccaccgaaa tcagcgaact gatcaagcag cgcattgctc agttcaatgt
    10921 tgtgagtgaa gctcacaacg aaggtactat tgtttctgta agtgacggtg ttatccgcat
    10981 tcacggcctg gccgattgta tgcagggtga aatgatctcc ctgccgggta accgttacgc
    11041 tatcgcactg aacctcgagc gcgactctgt aggtgcggtt gttatgggtc cgtacgctga
    11101 ccttgccgaa ggcatgaaag ttaagtgtac tggccgtatc ctggaagttc cggttggccg
    11161 tggcctgctg ggccgtgtgg ttaacactct gggtgcacca atcgacggta aaggtccgct
    11221 ggatcacgac ggcttctctg ctgtagaagc aatcgctccg ggcgttatcg aacgtcagtc
    11281 cgtagatcag ccggtacaga ccggttataa agccgttgac tccatgatcc caatcggtcg
    11341 tggtcagcgt gaattgatca tcggtgaccg tcagacaggt aaaaccgcac tggctatcga
    11401 tgccatcatc aaccagcgcg attccggtat caaatgtatc tatgtcgcta tcggccagaa
    11461 agcgtccacc atttctaacg tggtacgtaa actggaagag cacggcgcac tggctaacac
    11521 catcgttgtg gtagcaaccg cgtctgaatc cgctgcactg caatacctgg caccgtatgc
    11581 cggttgcgca atgggcgaat acttccgtga ccgcggtgaa gatgcgctga tcatttacga
    11641 tgacctgtct aaacaggctg ttgcttaccg tcagatctcc ctgctgctcc gtcgtccgcc
    11701 aggacgtgaa gcattcccgg gcgacgtttt ctacctccac tctcgtctgc tggagcgtgc
    11761 tgcacgtgtt aacgccgaat acgttgaagc cttcaccaaa ggtgaagtga aagggaaaac
    11821 cggttctctg accgcactgc cgattatcga aactcaggcg ggtgacgttt ctgcgttcgt
    11881 tccgaccaac gtaatctcca ttaccgatgg tcagatcttc ctggaaacca acctgttcaa
    11941 cgccggtatt cgtcctgcgg ttaacccggg tatttccgta tcccgtgttg gtggtgcagc
    12001 acagaccaag atcatgaaaa aactgtccgg tggtatccgt accgctctgg cacagtatcg
    12061 tgaactggca gcgttctctc agtttgcatc cgaccttgac gatgcaacac gtaagcagct
    12121 tgaccacggt cagaaagtga ccgaactgct gaaacagaaa cagtatgcgc cgatgtccgt
    12181 tgcgcagcag tctctggttc tgttcgcagc agaacgtggt tacctggcgg atgttgaact
    12241 gtcgaaaatt ggcagcttcg aagccgctct gctggcttac gtcgaccgtg atcacgctcc
    12301 gttgatgcaa gagatcaacc agaccggtgg ctacaacgac gaaatcgaag gcaagctgaa
    12361 aggcatcctc gattccttca aagcaaccca atcctggtaa cgtctggagg cttgccttag
    12421 ggcaggccgc aaggcattga ggagaagctc atggccggcg caaaagagat acgtagtaag
    12481 atcgcaagcg tccagaacac gcaaaagatc actaaagcga tggagatggt cgccgcttcc
    12541 aaaatgcgta aatcgcagga tcgcatggcg gccagccgtc cttatgcaga aaccatgcgc
    12601 aaagtgattg gtcaccttgc acacggtaat ctggaatata agcaccctta cctggaagac
    12661 cgcgacgtta aacgcgtggg ctacctggtg gtgtcgaccg accgtggttt gtgcggtggt
    12721 ttgaacatta acctgttcaa aaaactgctg gcggaaatga agacctggac cgacaaaggc
    12781 gttcaatgcg acctcgcaat gatcggctcg aaaggcgtgt cgttcttcaa ctccgtgggc
    12841 ggcaatgttg ttgcccaggt caccggcatg ggggataacc cttccctgtc cgaactgatc
    12901 ggtccggtaa aagtgatgtt gcaggcctac gacgaaggcc gtctggacaa gctttacatt
    12961 gtcagcaaca aatttattaa caccatgtct caggttccga ccatcagcca gctgctgccg
    13021 ttaccggcat cagatgatga tgatctgaaa cataaatcct gggattacct gtacgaaccc
    13081 gatccgaagg cgttgctgga taccctgctg cgtcgttatg tcgaatctca ggtttatcag
    13141 ggcgtggttg aaaacctggc cagcgagcag gccgcccgta tggtggcgat gaaagccgcg
    13201 accgacaatg gcggcagcct gattaaagag ctgcagttgg tatacaacaa agctcgtcag
    13261 gccagcatta ctcaggaact caccgagatc gtctcggggg ccgccgcggt ttaaacaggt
    13321 tatttcgtag aggatttaag atggctactg gaaagattgt ccaggtaatc ggcgccgtag
    13381 ttgacgtcga attccctcag gatgccgtac cgcgcgtgta cgatgctctt gaggtgcaaa
    13441 atggtaatga gcgtctggtg ctggaagttc agcagcagct cggcggcggt atcgtgcgta
    13501 ccatcgcaat gggttcctcc gacggtctgc gtcgcggtct ggatgtaaaa gacctcgaac
    13561 acccgatcga agtcccggta ggtaaagcga ctctgggccg tatcatgaac gtactgggtg
    13621 aaccggtcga catgaaaggc gagatcggtg aagaagagcg ttgggcgatt caccgcgcag
    13681 caccttccta cgaagagctg tcaaactctc aggaactgct ggaaaccggt atcaaagtta
    13741 tcgacctgat gtgtccgttc gctaagggcg gtaaagttgg tctgttcggt ggtgcgggtg
    13801 taggtaaaac cgtaaacatg atggagctca ttcgtaacat cgcgatcgag cactccggtt
    13861 actctgtgtt tgcgggcgta ggtgaacgta ctcgtgaggg taacgacttc taccacgaaa
    13921 tgaccgactc caacgttatc gacaaagtat ccctggtgta tggccagatg aacgagccgc
    13981 cgggaaaccg tctgcgcgtt gctctgaccg gtctgaccat ggctgagaaa ttccgtgacg
    14041 aaggtcgtga cgttctgctg ttcgttgaca acatctatcg ttacaccctg gccggtacgg
    14101 aagtatccgc actgctgggc cgtatgcctt cagcggtagg ttatcagccg accctggcgg
    14161 aagagatggg cgttctgcag gaacgtatca cctccaccaa aactggttct atcacctccg
    14221 tacaggcggt atacgtacct gcggatgact tgactgaccc gtctccggca accacctttg
    14281 cgcaccttga cgcaaccgtg gtactgagcc gtcagatcgc gtctctgggt atctacccgg
    14341 ccgttgaccc gctggactcc accagccgtc agctggaccc gctggtggtt ggtcaggaac
    14401 actacgacac cgcgcgtggc gttcagtcca tcctgcaacg ttatcaggaa ctgaaagaca
    14461 tcatcgccat cctgggtatg gatgaactgt ctgaagaaga caaactggtg gtagcgcgtg
    14521 ctcgtaagat ccagcgcttc ctgtcccagc cgttcttcgt ggcagaagta ttcaccggtt
    14581 ctccgggtaa atacgtctcc ctgaaagaca ccatccgtgg ctttaaaggc atcatggaag
    14641 gcgaatacga tcacctgccg gagcaggcgt tctacatggt cggttccatc gaagaagctg
    14701 tggaaaaagc caaaaaactt taacgcctta atcggagggt gatatggcaa tgacttacca
    14761 cctggacgtc gtcagcgcag agcaacaaat gttctctggt ctggtcgaga aaatccaggt
    14821 aacgggtagc gaaggtgaac tggggatcta ccctggccac gcaccgctgc tcaccgccat
    14881 taagcctggt atgattcgca tcgtgaaaca gcacggtcac gaagagttta tctatctgtc
    14941 tggcggcatt cttgaagtgc agcctggcaa cgtgaccgtt ctggccgaca ccgcaattcg
    15001 cggccaggat ctcgacgaag cgcgagccat ggaagcgaaa cgtaaggctg aagagcacat
    15061 tagcagctct cacggcgacg tagattacgc tcaggcgtct gcggaactgg ccaaagcgat
    15121 cgcgcagctg cgcgttatcg agttgaccaa aaaagcgatg taacaccggc ttgaaaagca
    15181 caaaagccag tctggaaaca ggctggcttt ttttacgcgt tgacccgtcc tgaagagcgt
    15241 tcacatagat cctgctgata taaaacaccc ctgttttccc gtttattcat tgatcgaaat
    15301 aagagcaaaa acatccacct gacgcttaaa tgaaggtact gccttaattt tctgcagata
    15361 aaaggcgtga cgatggtcga aaatggcact ttcgtcagcg gggataatcc gttattgaac
    15421 aatttatcct ctgtccattt cacgatgaaa aaaatgtagt tttttcaagg tgaagcggtt
    15481 taaattcgtt ctcaaattac agtcaggacg cgtatgttga ataatgctat gagcgtagtg
    15541 atccttgccg caggcaaagg cacgcgcatg tattccgatc ttccgaaagt gctgcatacc
    15601 cttgccggga aagcgatggt tcagcatgtc attgatgctg cgaatgaatt aggcgcagcg
    15661 cacgttcacc tggtgtacgg tcacggcggc gatctgctta aacaggcgct gaaagacgac
    15721 aaccttaact gggtgcttca ggcagagcag ctgggtacgg gtcatgcaat gcagcaggcc
    15781 gcacctttct ttgccgatga tgaagacatt ttaatgctct acggcgacgt gccgctgatc
    15841 tctgtcgaaa cactccagcg cctgcgtgat gctaaacccc agggtggcat tggtctgctg
    15901 acggtgaaac tggatgatcc gaccggttat ggacgtatca cccgtgaaaa tggcaaagtt
    15961 accggcattg ttgagcacaa agatgccacc gacgagcagc gtcagattca ggagatcaac
    16021 accggcattc tgattgccaa cggcgcagat atgaaacgct ggctggcgaa gctgaccaac
    16081 aataatgctc agggcgaata ctacatcacc gacattattg cgctggcgta tcaggaaggg
    16141 cgtgaaatcg tcgccgttca tccgcaacgt ttaagcgaag tagaaggtgt gaataaccgc
    16201 ctgcaactct cccgtctgga gcgcgtttac cagtccgaac aggctgaaaa actgctgtta
    16261 gcaggcgtta tgctgcgcga tccggcgcgt tttgatctgc gcggtacgct tactcacggg
    16321 cgcgatgttg aaattgatac taacgttatc atcgagggca acgtgactct cggtcatcgc
    16381 gtgaaaatcg gcaccggttg cgtgattaaa aacagcgtga ttggcgatga ttgcgaaatc
    16441 agtccgtata ccgttgtgga agacgcgaat ctggcagcgg cctgtactat tggcccgttt
    16501 gcccgtctgc gtcctggtgc tgagttgctg gaaggtgcac acgttggtaa cttcgttgag
    16561 atgaaaaaag cgcgtctggg taaaggcacg aaagccgggc atctgactta cctgggcgat
    16621 gcggaaattg gcgataacgt taacatcggc gcgggaacca ttacctgcaa ctacgatggt
    16681 gcgaataaat ttaagaccat tatcggcgac gatgtgtttg tcggttccga cactcagctg
    16741 gtggccccgg taacagtagg caaaggcgcg accattgctg cgggtacaac tgtgacgcgt
    16801 aatgtcggcg aaaatgcatt agctatcagc cgtgtgccgc agactcagaa agaaggctgg
    16861 cgtcgtccgg taaagaaaaa gtaattctgg ccgggtaatc cggtcatctg ggatgaggag
    16921 ataacataat ctccctccca caagcagtaa ctataaaaat aaccccactc tctacaaggc
    16981 tcggggcgcc cgaaaaaacg ggcatacagg ttgaccgaca acgatataaa tcggaatcaa
    17041 aaactatgtg tggaattgtt ggcgcgatcg cgcaacgtga tgtagcagaa atccttcttg
    17101 aaggtttacg tcgtctggaa taccgcggat atgactctgc cggtctggcc gttgttgatg
    17161 cggaaggtca tatgactcgc ctgcgtcgcc tcggtaaagt ccagatgctg gctcaggcag
    17221 cggaagaaca tcctctgcat ggcggcaccg gtattgctca cactcgctgg gcgacccacg
    17281 gtgaaccttc agaagctaac gcgcatccgc atgtttctga acacattgtg gtggtgcata
    17341 atggcatcat cgaaaaccat gaaccgctgc gtgaagagtt aaaagcgcgt ggctatacct
    17401 tcgtttctga aaccgacacc gaagtgattg cccatctggt gaactgggag ctgaaacaag
    17461 gcgggactct gcgtgaggct gttctgcgtg ctatcccgca gctgcgcggt gcgtacggta
    17521 cagtgatcat ggactcccgt cacccggata ccctgctagc ggcacgttct ggtagtccgc
    17581 tggtgattgg cctgggtatg ggcgaaaact ttatcgcttc tgaccagctg gcgctgttgc
    17641 cggtgacccg tcgctttatc ttccttgaag agggcgatat tgcggaaatc actcgccgtt
    17701 cggtaaacat cttcgataaa actggcgcgg aagtaaaacg tcaggatatc gaatccaatc
    17761 tgcaatatga cgcgggcgat aaaggtattt accgtcacta catgcagaaa gagatctacg
    17821 aacagccgaa cgcgataaaa aacaccctta ccgggcgcat cagccacggt caggttgatt
    17881 taagcgagct gggaccgaac gccgacgaac tgctgtcgaa ggttgagcat attcagatcc
    17941 tcgcctgtgg tacttcttat aactccggta tggtttcccg ctactggttt gaatcgctgg
    18001 caggtattcc gtgcgacgtc gaaatcgcct ctgaattccg ctatcgtaaa tctgccgtgc
    18061 gtcgtaacag cctgatgatc accttgtcac agtctggcga aaccgcggat accctggcgg
    18121 gcctgcgtct gtcgaaagag ctgggttacc ttggttcgct ggcaatctgt aacgttccgg
    18181 gttcttctct ggtgcgcgaa tccgatctgg cgctgatgac caacgcgggt acggaaatcg
    18241 gcgtggcgtc cactaaagca ttcaccactc agttaactgt gctgttgatg ctggtggcga
    18301 agctgtctcg cctgaaaggt ctggatgcct ccattgaaca tgacattgtg catggtctgc
    18361 aggcgctgcc gagccgtatt gagcagatgc tgtctcagga taaacgcatt gaagctctgg
    18421 cagaagattt ctctgacaaa catcacgcgc tgttcctggg ccgtggcgat cagtacccaa
    18481 tcgcgctgga aggcgcattg aagctgaaag agatctctta cattcacgct gaagcctacg
    18541 ctgcaggcga gctgaaacac ggcccgctgg cgctgattga cgcggatatg ccggttatcg
    18601 tcgttgcacc gaacaacgaa ctgttggaaa aactgaaatc caacattgaa gaagttcgcg
    18661 cgcgtggcgg tcaattgtat gtcttcgccg atcaggatgc gggttttgta agtagcgata
    18721 acatgcacat catcgagatg ccgcatgtgg aagaggtgat tgcaccgatc ttctacaccg
    18781 ttccgctgca gctgctggct taccatgtcg cgctgatcaa aggcaccgac gttgaccagc
    18841 cgcgtaacct ggcaaaatcg gttacggttg agtaatcact atcatagata aaaaagggct
    18901 gctgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
    18961 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
    19021 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
    19081 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattccat ggctaacagt
    19141 acaagaagtt ccttcttcag gtcgttccca ccgtatttat tctcataaga cgggacgagt
    19201 ccatcatttg ctatctgact tagagcttgc tgtttttctc agtcttgagt gggagagcag
    19261 cgtgctagat atacgcgagc agttcccctt attacctagt gataccaggc agattgcaat
    19321 agatagtggt attaagcatc ctgttattcg tggtgtagat caggttatgt ctactgattt
    19381 tttagtggac tgcaaagatg gtccttttga gcagtttgct attcaagtca aacctgcagc
    19441 agccttacaa gacgagcgta ccttagaaaa actagaacta gagcgtcgct attggcagca
    19501 aaagcaaatt ccttggttca tttttactga taaagaaata aatcccgtag taaaagaaaa
    19561 tattgaatgg ctttattcag tgaaaacaga agaagtttct gcggagcttt tagcacaact
    19621 atccccattg gcccatatcc tgcaagaaaa aggagatgaa aacattatca atgtctgtaa
    19681 gcaggttgat attgcttatg atttggagtt aggcaaaaca ttgagtgaga tacgagcctt
    19741 aaccgcaaat ggttttatta agttcaatat ttataagtct ttcagggcaa ataagtgtgc
    19801 agatctctgt attagccaag tagtgaatat ggaggagttg cgctatgtgg caaattaatg
    19861 aggttgtgct atttgataat gatccgtatc gcattttggc tatagaggat ggccaagttg
    19921 tctggatgca aataagcgct gataaaggag ttccacaagc tagggctgag ttgttgctaa
    19981 tgcagtattt agatgaaggc cgcttagtta gaactgatga cccttatgta catcttgatt
    20041 tagaagagcc gtctgtagat tctgtcagct tccagaagcg cgaggaggat tatcgaaaaa
    20101 ttcttcctat tattaatagt aaggatcgtt tcgaccctaa agtcagaagc gaactcgttg
    20161 agcatgtggt ccaagaacat aaggttacta aggctacagt ttataagttg ttacgccgtt
    20221 actggcagcg tggtcaaacg cctaatgcat taattcctga ctacaaaaac agcggtgcac
    20281 caggggaaag acgttcagcg acaggaacag caaagattgg ccgagccaga gaatatggta
    20341 agggtgaagg aaccaaggta acgcccgaga ttgaacgcct ttttaggttg accatagaaa
    20401 agcacctgtt aaatcaaaaa ggtacaaaga ccaccgttgc ctatagacga tttgtggact
    20461 tgtttgctca gtattttcct cgcattcccc aagaggatta cccaacacta cgtcagtttc
    20521 gttattttta tgatcgagaa taccctaaag ctcagcgctt aaagtctaga gttaaagcag
    20581 gggtatataa aaaagacgta cgacccttaa gtagtacagc cacttctcag gcgttaggcc
    20641 ctgggagtcg ttatgagatt gatgccacga ttgctgatat ttatttagtg gatcatcatg
    20701 atcgccaaaa aatcatagga agaccaacgc tttacattgt gattgatgtg tttagtcgga
    20761 tgatcacggg cttttatatc ggctttgaaa atccgtctta tgtggtggcg atgcaggctt
    20821 ttgtaaatgc ttgctctgac aaaacggcca tttgtgccca gcatgatatt gagattagta
    20881 gctcagactg gccgtgtgta ggtttgccag atgtgttgct agcggaccgt ggcgaattaa
    20941 tgagtcatca ggtcgaagcc ttagtttcta gttttaatgt gcgagtggaa agtgctccac
    21001 ctagacgtgg cgatgctaaa ggcatagtgg aaagcacttt tagaacacta caagccgagt
    21061 ttaagtcctt tgcacctggc attgtagagg gcagtcggat caaaagccat ggtgaaacag
    21121 actataggtt agatgcatct ctgtcggtat ttgagttcac acaaattatt ttgcgtacga
    21181 tcttattcag aaataaccat ctggtgatgg ataaatacga tcgagatgct gattttccta
    21241 cagatttacc gtctattcct gtccagctat ggcaatgggg tatgcagcat cgtacaggta
    21301 gtttaagggc tgtggagcaa gagcagttgc gagtagcgtt actgcctcgc cgaaaggtct
    21361 ctatttcttc atttggcgtt aatttgtggg gtttgtatta ctcggggtca gagattctgc
    21421 gtgagggttg gttgcagcgg agcactgata tagctagacc tcaacattta gaagcggctt
    21481 atgacccagt gctggttgat acgatttatt tgtttccgca agttggcagc cgtgtatttt
    21541 ggcgctgtaa tctgacggaa cgtagtcggc agtttaaagg tctctcattt tgggaggttt
    21601 gggatataca agcacaagaa aaacacaata aagccaatgc gaagcaggat gagttaacta
    21661 aacgcaggga gcttgaggcg tttattcagc aaaccattca gaaagcgaat aagttaacgc
    21721 ccagtactac tgagcccaaa tcaacacgca ttaagcagat taaaactaat aaaaaagaag
    21781 ccgtgacctc ggagcgtaaa aaacgtgcgg agcatttgaa gccaagctct tcaggtgatg
    21841 aggctaaagt tattcctttc aacgcagtgg aagcggatga tcaagaagat tacagcctac
    21901 ccacatacgt gcctgaatta tttcaggatc caccagaaaa ggatgagtca tgagtgctac
    21961 ccggattcaa gcagtttatc gtgatacggg ggtagaggct tatcgtgata atccttttat
    22021 cgaggcctta ccaccattac aagagtcagt gaatagtgct gcatcactga aatcctcttt
    22081 acagcttact tcctctgact tgcaaaagtc ccgtgttatc agagctcata ccatttgtcg
    22141 tattccagat gactattttc agccattagg tacgcatttg ctactaagtg agcgtatttc
    22201 ggtcatgatt cgaggtggct acgtaggcag aaatcctaaa acaggagatt tacaaaagca
    22261 tttacaaaat ggttatgagc gtgttcaaac gggagagttg gagacatttc gctttgagga
    22321 ggcacgatct acggcacaaa gcttattgtt aattggttgt tctggtagtg ggaagacgac
    22381 ctctcttcat cgtattctag ccacgtatcc tcaggtgatt taccatcgtg aactcaatgt
    22441 agagcaggtg gtgtatttga aaatagactg ctcgcataat ggttcgctaa aagaaatctg
    22501 cttgaatttt ttcagagcgt tggatcgagc cttgggctcg aactatgagc gtcgttatgg
    22561 cttaaaacgt catggtatag aaaccatgtt ggctttgatg tcgcaaatag ccaatgcaca
    22621 tgctttaggg ttgttggtta ttgatgaaat tcagcattta agccgctctc gttcgggtgg
    22681 atctcaagag atgctgaact tttttgtgac gatggtgaat attattggcg taccagtgat
    22741 gttgattggt acccctaaag cacgagagat ttttgaggct gatttgcggt ctgcacgtag
    22801 aggggcaggg tttggagcta tattctggga tcctatacaa caaacgcaac gtggaaagcc
    22861 caatcaagag tggatcgctt ttacggataa tctttggcaa ttacagcttt tacaacgcaa
    22921 agatgcgctg ttatcggatg aggtccgtga tgtgtggtat gagctaagcc aaggagtgat
    22981 ggacattgta gtaaaacttt ttgtactcgc tcagctccgt gcgctagctt taggcaatga
    23041 gcgtattacc gctggtttat tgcggcaagt gtatcaagat gagttaaagc ctgtgcaccc
    23101 catgctagag gcattacgct cgggtatccc agaacgcatt gctcgttatt ctgatctagt
    23161 cgttcccgag attgataaac ggttaatcca acttcagcta gatatcgcag cgatacaaga
    23221 acaaacacca gaagaaaaag cccttcaaga gttagatacc gaagatcagc gtcatttata
    23281 tctgatgctg aaagaggatt acgattcaag cctgttaatt cccactatta aaaaagcgtt
    23341 tagccagaat ccaacgatga caagacaaaa gttactgcct cttgttttgc agtggttgat
    23401 ggaaggcgaa acggtagtgt cagaactaga aaagccctcc aagagtaaaa aggtttcggc
    23461 tataaaggta gtcaagccca gcgactggga tagcttgcct gatacggatt tacgttatat
    23521 ctattcacaa cgccaacctg aaaaaaccat gcatgaacgg ttaaaaggga aaggggtaat
    23581 agtggatatg gcgagcttat ttaaacaagc aggttagcca tgagaaactt tcctgttccg
    23641 tactcgaatg agctgattta tagcactatt gcacgggcag gcgtttatca agggattgtt
    23701 agtcctaagc agctgttgga tgaggtgtat ggcaaccgca aggtggtcgc taccttaggt
    23761 ctgccctcgc atttaggtgt gatagcaaga catctacatc aaacaggacg ttacgctgtt
    23821 cagcagctta tttatgagca taccttattc cctttatatg ctccgtttgt aggcaaggag
    23881 cgccgagacg aagctattcg gttaatggag taccaagcgc aaggtgcggt gcatttaatg
    23941 ctaggagtcg ctgcttctag agttaagagc gataaccgct ttagatactg ccctgattgc
    24001 gttgctcttc agctaaatag gtatggggaa gccttttggc aacgagattg gtatttgccc
    24061 gctttgccat attgtccaaa acacggtgct ttagtcttct ttgatagagc tgtagatgat
    24121 caccgacatc aattttgggc tttgggtcat actgagctgc tttcagacta ccccaaagac
    24181 tccctatctc aattaacagc actagctgct tatatagccc ctctgttaga tgctccacga
    24241 gcgcaagagc tttccccaag ccttgagcag tggacgctgt tttatcagcg cttagcgcag
    24301 gatctagggc taaccaaaag caagcacatt cgtcatgact tggtggcgga gagagtgagg
    24361 cagactttta gtgatgaggc actagagaaa ctggatttaa agttggcaga gaacaaggac
    24421 acgtgttggc tgaaaagtat attccgtaag catagaaaag cctttagtta tttacagcat
    24481 agtattgtgt ggcaagcctt attgccaaaa ctaacggtta tagaagcgct acagcaggca
    24541 agtgctctta ctgagcactc tataacgaca agacctgtta gccagtctgt gcaacctaac
    24601 tctgaagatt tatctgttaa gcataaagac tggcagcaac tagtgcataa ataccaagga
    24661 attaaggcgg caagacagtc tttagagggt ggggtgctat acgcttggct ttaccgacat
    24721 gacagggatt ggctagttca ctggaatcaa cagcatcaac aagagcgtct ggcacccgcc
    24781 cctagagttg attggaacca aagagatcga attgctgtac gacaactatt aagaatcata
    24841 aagcgtctag atagtagcct tgatcaccca agagcgacat cgagctggct gttaaagcaa
    24901 actcctaacg gaacctctct tgcaaaaaat ctacagaaac tgcctttggt agcgctttgc
    24961 ttaaagcgtt actcagagag tgtggaagat tatcaaatta gacggattag ccaagctttt
    25021 attaagctta aacaggaaga tgttgagctt aggcgctggc gattattaag aagtgcaacg
    25081 ttatctaaag agcggataac tgaggaagca caaagattct tggaaatggt ttatggggaa
    25141 gagtgagtgg ttaggctagc tacatttaat gacaatgtgc aggttgtaca tattggtcat
    25201 ttattccgta actcgggtca taaggagtgg cgtatttttg tttggtttaa tccaatgcaa
    25261 gaacggaaat ggactcgatt tactcatttg cctttattaa gtcgagctaa ggtggttaac
    25321 agtacaacaa agcaaataaa taaggcggat cgtgtgattg agtttgaagc atcggatctt
    25381 caacgagcca aaataatcga ttttcctaat ctctcgtcct ttgcttccgt acgcaacaag
    25441 gatggagcgc agagttcatt tatttacgaa gctgaaacac catatagcaa gactcgttat
    25501 cacatcccac agttagagct agctcggtca ttatttttaa ttaactccta tttctgtcga
    25561 agctgtttga gcagtaccgc tttacagcaa gagttcgacg ttcagtatga ggttgagcga
    25621 gatcatttag agataaggat cttacccagt tcatcgtttc ctaaaggggc gttagagctg
    25681 tcggccgtag tgcagctttt ggtttggttg ttttcggatc aagatgttat ggattcgtat
    25741 gaaagtattt ttaggcacta tcaacaaaat agagaaatta agaacggcgt tgaaagctgg
    25801 tgctttagct ttgaccctcc gcccatgcag ggttggaaat tacatgtaaa aggacgttct
    25861 tctaacgagg ataaggatta tttagttgag gaaatagtag gtttagaaat caacgctatg
    25921 cttcctagca caacagctat tagccatgcc tcttttcagg aaaaggaggc aggtgatggt
    25981 agtacgcagc acatagcggt ttcaacagag tcagttgttg atgatgagca tctacagttg
    26041 gacgatgagg aaacagccaa tatagacaca gacacacgag tcatagaggc tgagccgaca
    26101 tggataagtt ttagtagacc tagtcgaatt gaaaaatctc gcagggcaag aaaaagtagc
    26161 caaactattt tagaaaaaga agaagcaaca acaagtgaaa atagtaattt ggttagtact
    26221 gatgagccac acttaggtgg tgtcctagca gcggcagatg tgggtgggaa gcaggatgca
    26281 accaattaca actctatttt tgctaatcga tttgctgctt ttgatgagct actttcaatt
    26341 ctaaaaacta aatttgcatg tcgggtgctt tttgaagaaa ccttggtttt gccaaaagtt
    26401 gggcgtagcc gattacatct gtgtaaagat ggctcaccaa gagtgattaa agccgttggg
    26461 gtgcaacgta atggcagtga atttgtattg ctagaggtgg atgcatcgga tggggtgaaa
    26521 atgctttcta ccaaagtgtt gagtggcgtt gatagcgaaa catggcggaa tgattttgaa
    26581 aagatacggc gtggagtggt gaagagctca ttgaattggc caaatagttt gtttgatcaa
    26641 ttatatggac aagacgggca tagaggggtg aatcatccaa aggggttggg ggagctgcaa
    26701 gtatcgagag aggatatgga agggtgggct gagagagtgg ttagagagca atttacgcat
    26761 taaaggaatg actgaaagag cctgtaaacc cttttgtgta agtgcttttg ccggtcagtt
    26821 aaaggtggcc atttaaacgg tcaccaaatt cgatcataaa acggttcatg gccggcttcc
    26881 agttgcggat cggcatcgtc catttcttgg tcgccgcctg gatagccagg tacaccacct
    26941 tcatcgctga ttcgtccgta gggaacacct tgagttcata ttaatggaat tttctacaaa
    27001 tagcctccgt ggttttgagg ggggattaca gacgatccat agtagtaatc caatgagttc
    27061 ttgagcgcgg cgacatgttt ggacgccttg gcaaaaatta gagcctgctt gaagtgcagg
    27121 cgagcacgtg cttggacgat tgatccattc gcggtcaaaa actcaatctt ggatgacagc
    27181 gtgtcaggaa atccaatatc gaagtcccgc cccgtgtcaa agtagggcat ttcatgatca
    27241 aaggacgaag cttcccaggc cacttcccta atattccagc cgctagaaac aagatttcgt
    27301 acgacttcat caactttttt gctttccaac ttgtcacaaa gtgcataggc aagagctttg
    27361 gcggcagaca cgtgcgtttc ctcttaaaca taggtcgaac ggatacccga ggcaaagcgg
    27421 agcattagtc ccaaagcatc tccggtagat ccggaggctc catactgaag gcatggcatc
    27481 ttgggcattg atatttgaaa cttctctcta gatagcaggg atttcttggc cttgcctcat
    27541 gctgatagct tgtaaatatc acacgatcct tagccccaca aagtgagcat actccgtagc
    27601 tttcatttat agaaatcgtc gttagattgt cgcagcatgt gcacgttccg actatttgct
    27661 caggaagcat cgccatacct tggcctaggt aaagaagatc agactcgtag ccacatcggt
    27721 ggcaactgag cttgtacgca atgcccatct ggttgtctca cctgaatcgt aacgtcagag
    27781 ctattcatcc atccattcgg catcaatgta tgccactaag ccgaccagct gaccgtttgg
    27841 actgaagtat gttgcctgca ccccacttga catctcatca ataccaccgc actgcgctag
    27901 gcgccggtcc cacttgccca cagaagcgtc aggatcggct gagttcccca agacccttgt
    27961 gagccatgcc tcctttaact caacctctcg aatgattgcg tcggccagct cctccgcgtt
    28021 atctagttcg acatagtctg gaattcggac gtacgttctg aacacagctg gatacttact
    28081 tgggcgattg tcatacatga catcaacaat gtacccgttt gtgtaaccgt ctcttggagg
    28141 ttcgtatgac actagtggtt cccctcagct tgcgactaga tgttgaggcc taacatttta
    28201 ttagagagca ggctagttgc ttagatacat gatcttcagg ccgttatctg tcagggcaag
    28261 cgaaaattgg ccatttatga cgaccaatgc cccgcagaag ctcccatctt tgccgccata
    28321 gacgccgcgc cccccttttg gggtgtagaa catccttttg ccagatgtgg aaaagaagtt
    28381 cgttgtccca ttgttggcaa tgacgtagta gccggcgaaa gtgcgagacc catttgcgct
    28441 atatataagc ctacgatttc cgttgcgact attgtcgtaa ttggatgaac tattatcgta
    28501 gttgctctca gagttgtcgt aatttgatgg actattgtcg taattgctta tggagttgtc
    28561 gtagttgctt ggagaaatgt cgtagttgga tggggagtag tcatagggaa gacgagcttc
    28621 atccactaaa acaattggca ggtcagcaag tgcctgcccc gatgccatcg caagtacgag
    28681 gcttagaacc accttcaaca gatcgcgcat agtcttcccc agctctctaa cgcttgagtt
    28741 aagccgcgcc gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac
    28801 cttggtgatc tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc
    28861 caagcgatct tcttgtccaa gataagcctg cctagcttca agtatgacgg gctgatactg
    28921 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
    28981 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
    29041 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
    29101 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
    29161 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
    29221 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
    29281 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
    29341 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
    29401 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
    29461 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
    29521 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
    29581 taactcctga attaagccgc gccgcgaagc ggtgtcggct tgaatgaatt gttaggcgtc
    29641 atcctgtgct cccgagaacc agtaccagta catcgctgtt tcgttcgaga cttgaggtct
    29701 agttttatac gtgaacaggt caatgccgcc gagagtaaag ccacattttg cgtacaaatt
    29761 gcaggcaggt acattgttcg tttgtgtctc taatcgtatg ccaaggagct gtctgcttag
    29821 tgcccacttt ttcgcaaatt cgatgagact gtgcgcgact cctttgcctc ggtgcgtgtg
    29881 cgacacaaca atgtgttcga tagaggctag atcgttccat gttgagttga gttcaatctt
    29941 cccgacaagc tcttggtcga tgaatgcgcc atagcaagca gagtcttcat cagagtcatc
    30001 atccgagatg taatccttcc ggtaggggct cacacttctg gtagatagtt caaagccttg
    30061 gtcggatagg tgcacatcga acacttcacg aacaatgaaa tggttctcag catccaatgt
    30121 ttccgccacc tgctcaggga tcaccgaaat cttcatatga cgcctaacgc ctggcacagc
    30181 ggatcgcaaa cctggcgcgg cttttggtac aaaaggcgtg acaggtttgc gaatccgttg
    30241 ctgccacttg ttaacccttt tgccagattt ggtaactata atttatgtta gaggcgaagt
    30301 cttgggtaaa aactggccta aaattgctgg ggatttcagg aaagtaaaca tcaccttccg
    30361 gctcgatgtc tattgtagat atatgtagtg tatctacttg atcgatcagg cttttgtata
    30421 tctccccacc acctgaaaca atgacatgat ccgttatttt ctttaggttg gttaaagcat
    30481 ctttaattga tggaaagatc aatacgttct cattgtcaga tgtaaaactt gaacgtgtta
    30541 cgaccgcata ctttcggttg ggtaatgctc ccattgattc aaaagtcttg cgtccaacca
    30601 acagccattg gttataggta atagctttaa acaggagctg ttcacctttg gcactccatg
    30661 gaatatcagg gccattcccg ataactccat tcttcgatat agctaccatt agtgatagtt
    30721 tcacaattct tcctcagagg ttaacatttt attagtaagc atgctcgttt tgtcacccgc
    30781 tgatgcttac cgttaattaa tataatcact tgtattaaat agactttctt taaaaataca
    30841 agacactctg ttattacaaa tcgtgcatgc cgtctatcct gcttgcacga tgcacattat
    30901 ctcacccaaa gaactcataa cattttaatt tatttagcat taaattaaaa ctctaccggt
    30961 aaacaagcat ctctaggcgt aaaaaacagg cactttttta aatcccagct agaataggct
    31021 gtataggcag acagttgcaa gacaaggagc gttttatgtc taacagtcca tttttaaatt
    31081 ctatacgcac ggatatgcga caaaaaggtt atgcgctgaa aactgaaaaa acttacctgc
    31141 actggattaa gcgttttatt ctgtttcaca aaaaacgtca tcctcagacc atgggcagtg
    31201 aagaggtcag gctgttttta tccagcttag caaacagcag acatgtagcc ataaacacgc
    31261 agaaaatcgc tttaaatgcc ctagcttttt tgtacaacag gtttttacaa cagccgttgg
    31321 gcgatattga ttatatccct gcaagcaagc ctagacggct accctctgtt atctctgcaa
    31381 atgaagtgca acgcattttg caggttatgg atactcgcaa ccaagttatt tttacgctgc
    31441 tgtatggtgc aggtttgcgc attaatgaat gcttgcgttt gcgggttaaa gattttgatt
    31501 ttgataatgg ctgcatcact gtgcatgacg gtaagggtgg gaaaagcaga aacagcctac
    31561 tgcccacgcg cctaatccca gcaataaaat aactcattga gcaagcgcgg cttattcagc
    31621 aagacgacaa cttacaaggc gtagggccat cgctgccttt tgctttagat cacaaatacc
    31681 cttctgctta tcgacaagcg gcgtggatgt ttgtctttcc ctccagcacg ctctgcaacc
    31741 acccgtataa cggcaaatta tgccgccatc atctgcatga ctccgttgcg cgaaaggcat
    31801 tgaaggcagc cgtacaaaaa gcaggcatcg ttagcaagcg tgtcacttgt catacatttc
    31861 gtcactcgtt tgctacgcat ctattacaag cggggcgtga tattcgcact gtgcaagaac
    31921 tcttagggca taacgatgtt aagaccacgc aaatctatac gcatgtgttg ggtcagcatt
    31981 ttgccggcac caccagtcct gcggatggac tgatgctact tatcaatcag taaaagtaga
    32041 taaatatctt tatgtagccc tgagtagccc ctgatacttt ttatggtgaa aaagataaac
    32101 aatcatagag gggataaata tgagccagtt ctaccattgt tcagataagt ttaagcaaga
    32161 tactgtcaat cacagtgacc attcatggtc attacacttc ctccagttgc cagacaatgc
    32221 ctaacataaa aacgcaaaag cccaaaagtc agagcttagg tgttctaggc gttttctaag
    32281 cgctgaagaa atacgtctta ccgtagattg cgtgcgtttc tgaagcacat tttccttata
    32341 catggcttta tcccaggcag cttcatcaag ctcttggagt aacaagccag taataatgcg
    32401 acactctcgg gtttgtagcg agccgctggt gagatcggag ttatagcgga aaggggtcat
    32461 ttgtaattat ttgtgggtgg aggatagtta acttattatt gtagttggaa gaggctgctt
    32521 tccaaacatt agcacctcgt aatgcttgtt actgtatatt aatttatgct cttagcgcat
    32581 gttaatgggc tattacaatg cataatgtcc acatcaagat aactgtggct taacacctgt
    32641 acaagcgaaa aaaatcaatc acctgtccgg aaatacggga tcactgtaaa gtgttggtga
    32701 ggcagtctag gagggggatt taatttggga tactgatgaa aggattatga tcagttggat
    32761 ataggatcgt actaagctga gcggcaggtt agctagatta gttaaccaga taagtgaaat
    32821 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
    32881 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
    32941 ccagttccca actattttgt ccgcccacag ctgccg
//
LOCUS       DAJTRN010000040        31446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRN010000040 DAJTRN010000000
VERSION     DAJTRN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO328_001919"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO328_001919"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5930505.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO328_001920"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO328_001920"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5930506.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO328_001921"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO328_001921"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5930507.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO328_001922"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO328_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5930508.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO328_001923"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO328_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5930509.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO328_001924"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO328_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5930510.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO328_001925"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO328_001925"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5930511.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO328_001926"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO328_001926"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5930512.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO328_001927"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO328_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5930513.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO328_001928"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO328_001928"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5930514.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO328_001929"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO328_001929"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5930515.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO328_001930"
     CDS             8212..9075
                     /locus_tag="OO328_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5930516.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO328_001931"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO328_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5930517.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO328_001932"
     CDS             10411..11028
                     /locus_tag="OO328_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5930518.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO328_001933"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO328_001933"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5930519.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO328_001934"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO328_001934"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5930520.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO328_001935"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO328_001935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5930521.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO328_001936"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO328_001936"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5930522.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO328_001937"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO328_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5930523.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO328_001938"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO328_001938"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5930524.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO328_001939"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO328_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5930525.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO328_001940"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO328_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5930526.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO328_001941"
     CDS             21859..23568
                     /locus_tag="OO328_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5930527.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO328_001942"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO328_001942"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5930528.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO328_001943"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO328_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO328_001944"
     tRNA            27625..27719
                     /locus_tag="OO328_001944"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO328_001945"
     CDS             27991..29202
                     /locus_tag="OO328_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5930529.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO328_001946"
     CDS             29614..30657
                     /locus_tag="OO328_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5930530.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..>31446
                     /locus_tag="OO328_001947"
     CDS             31172..>31446
                     /locus_tag="OO328_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930531.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVT"
BASE COUNT         7881 a         8288 c         7801 g         7476 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttac
//
LOCUS       DAJTRN010000041        30630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRN010000041 DAJTRN010000000
VERSION     DAJTRN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(34..240)
                     /locus_tag="OO328_001948"
     CDS             complement(34..240)
                     /locus_tag="OO328_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5930532.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            553..642
                     /gene="kdpF"
                     /locus_tag="OO328_001949"
     CDS             553..642
                     /gene="kdpF"
                     /locus_tag="OO328_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5930533.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            642..2315
                     /gene="kdpA"
                     /locus_tag="OO328_001950"
     CDS             642..2315
                     /gene="kdpA"
                     /locus_tag="OO328_001950"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5930534.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2338..4385
                     /gene="kdpB"
                     /locus_tag="OO328_001951"
                     /pseudo
     CDS             2338..4385
                     /gene="kdpB"
                     /locus_tag="OO328_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4394..4966
                     /gene="kdpC"
                     /locus_tag="OO328_001952"
     CDS             4394..4966
                     /gene="kdpC"
                     /locus_tag="OO328_001952"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5930535.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4959..7639
                     /gene="kdpD"
                     /locus_tag="OO328_001953"
                     /pseudo
     CDS             4959..7639
                     /gene="kdpD"
                     /locus_tag="OO328_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7636..8321
                     /gene="kdpE"
                     /locus_tag="OO328_001954"
                     /pseudo
     CDS             7636..8321
                     /gene="kdpE"
                     /locus_tag="OO328_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8596..8700
                     /locus_tag="OO328_001955"
     CDS             8596..8700
                     /locus_tag="OO328_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5930536.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9011..11208
                     /gene="speF"
                     /locus_tag="OO328_001956"
                     /pseudo
     CDS             9011..11208
                     /gene="speF"
                     /locus_tag="OO328_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11205..12524
                     /gene="potE"
                     /locus_tag="OO328_001957"
     CDS             11205..12524
                     /gene="potE"
                     /locus_tag="OO328_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5930537.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12580..14220)
                     /gene="pgm"
                     /locus_tag="OO328_001958"
     CDS             complement(12580..14220)
                     /gene="pgm"
                     /locus_tag="OO328_001958"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5930538.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14246..14791)
                     /gene="seqA"
                     /locus_tag="OO328_001959"
     CDS             complement(14246..14791)
                     /gene="seqA"
                     /locus_tag="OO328_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5930539.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14976..15740
                     /gene="ybfF"
                     /locus_tag="OO328_001960"
     CDS             14976..15740
                     /gene="ybfF"
                     /locus_tag="OO328_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5930540.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15880..16173
                     /gene="ybfE"
                     /locus_tag="OO328_001961"
     CDS             15880..16173
                     /gene="ybfE"
                     /locus_tag="OO328_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5930541.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16313..16843
                     /gene="fldA"
                     /locus_tag="OO328_001962"
     CDS             16313..16843
                     /gene="fldA"
                     /locus_tag="OO328_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5930542.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17053..17139
                     /gene="uof"
                     /locus_tag="OO328_001963"
     CDS             17053..17139
                     /gene="uof"
                     /locus_tag="OO328_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5930543.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17132..17578
                     /gene="fur"
                     /locus_tag="OO328_001964"
     CDS             17132..17578
                     /gene="fur"
                     /locus_tag="OO328_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5930544.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17662..17988)
                     /locus_tag="OO328_001965"
     CDS             complement(17662..17988)
                     /locus_tag="OO328_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930545.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18025..19431)
                     /gene="chiP"
                     /locus_tag="OO328_001966"
     CDS             complement(18025..19431)
                     /gene="chiP"
                     /locus_tag="OO328_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5930546.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20010..21674)
                     /gene="glnS"
                     /locus_tag="OO328_001967"
     CDS             complement(20010..21674)
                     /gene="glnS"
                     /locus_tag="OO328_001967"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5930547.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21877..23823)
                     /gene="nagE"
                     /locus_tag="OO328_001968"
     CDS             complement(21877..23823)
                     /gene="nagE"
                     /locus_tag="OO328_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5930548.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24156..24956
                     /gene="nagB"
                     /locus_tag="OO328_001969"
     CDS             24156..24956
                     /gene="nagB"
                     /locus_tag="OO328_001969"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5930549.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25016..26164
                     /gene="nagA"
                     /locus_tag="OO328_001970"
     CDS             25016..26164
                     /gene="nagA"
                     /locus_tag="OO328_001970"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5930550.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26173..27393
                     /gene="nagC"
                     /locus_tag="OO328_001971"
                     /gene_synonym="nagR"
     CDS             26173..27393
                     /gene="nagC"
                     /locus_tag="OO328_001971"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5930551.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27441..28193
                     /gene="nagD"
                     /locus_tag="OO328_001972"
                     /pseudo
     CDS             27441..28193
                     /gene="nagD"
                     /locus_tag="OO328_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28590..30254
                     /gene="asnB"
                     /locus_tag="OO328_001973"
     CDS             28590..30254
                     /gene="asnB"
                     /locus_tag="OO328_001973"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5930552.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         7264 a         7770 c         7999 g         7597 t
ORIGIN      
        1 cctgatacga ggctggctta agcgcgcagg caatcagtaa aaatcaccgg ttgccttttc
       61 ggcctgatcc atccacaccg gtttatcgct ggttttcgac cagacgcggt gcaggtagct
      121 gtaaaagcgg gcgcggtctt tccagaaaag catgaaaggc agcgccagaa cgcccgctgc
      181 aaccgcatat gtacggcgta aaaagataag ccatgcagga tattctctgt agagttccat
      241 acttttctcc cccttttgta tctacccggt gaatagcacc ggaaaaatga atttgtttat
      301 ctgatgaaaa tagtaccgcc ttttgtgtaa ttttactact catccgacca cttatttttg
      361 cttattgatg gtttatttac attcacactg taattaaatt acacaaaagt taaattaata
      421 ctaaccatca gttaaatcat ggcctttgcc atttttatac tttttttaca ccccgtccgc
      481 agatttttgc gaaatctttg cagccagaat tctactcttc cggtatcact tttaggccac
      541 tggaggtgca ctgtgagtgc aggcgtgata gccggcgtgt tgctggtgtt tttattactg
      601 ggctatttgg tttatgccct gatcaatgcg gaggcgttct gatggctgcg caagggttct
      661 tactgattgc cacgttttta ctggtattaa tggtgctggc gcgtccttta ggcagcgggc
      721 tggcgcggct gattaatgac attcctcttc caggtacagc gggcgttgaa cgtatactct
      781 ttcgtttacc tggcgtctct gaccatgaga tgaactggaa gcaatatctt tgtgccattc
      841 tcggcctgaa catgctgggg ctggcggtgc tgttttttat gttgctcggt cagcactatc
      901 tgccgcttaa tccacagcag ttgccggggc tgtcgtggga tctggcgctg aataccgccg
      961 tcagctttgt cacgaatacc aactggcaat cttatagcgg tgaaaccacg ttgagctatt
     1021 tcagccagat ggcaggctta acggtacaaa actttctttc tgccgccagc gggattgcgg
     1081 tgatttttgc cttcatccgt gcgtttactc gccagagcat gagcacactc gggaatgcct
     1141 gggtcgatct gctacgcatc acgttatggg tgctagtccc tgtggcgttg ttgatcgcac
     1201 tgttttttat tcaacaaggt gcgcagcaaa actttctgcc ctatcaggca gtgaataccg
     1261 ttgaaggagc gcaacagctg ttacccatgg gaccagtagc ttctcaggaa gcgatcaaga
     1321 tgctcggtac taacggcggt ggcttcttta atgccaactc gtcgcatccg tttgaaaacc
     1381 caaccgcact gaccaacttc gtgcagatgc tggcgatctt cttgatccca acagcgctgt
     1441 gctttgcctt tggtgaagtg acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga
     1501 tgtcagtgat ttttgtcatc tgcgtaggcg tggttatgtg ggcagaagtt cagggtaatc
     1561 ctcatctgct ggcgctcggc gcagacagca gcatcaatat ggaaggtaaa gagagccgtt
     1621 tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac ggcggcgtcc tgtggcgcgg
     1681 tgattgcgat gcatgattcg tttaccgctc tcggcggcat ggtgccgatg tggctaatgc
     1741 aaattggtga agtggtgttc ggcggtgtcg gttctggtct ttacggcatg atgctgtttg
     1801 tcctgctggc ggtgtttatt gccgggctga tgattggtcg tacaccggaa tatctgggta
     1861 aaaaaataga cgtacgcgag atgaaactga ctgcactggc gattctggtt actccaacac
     1921 tggtactgat gggcgcggcg ttggcgatga tgaccgacgc cggacgtagc gccatgctta
     1981 accctagccc gcatggtttt agcgaagtgc tgtacgccgt gtcgtccgcc gctaacaaca
     2041 acggcagcgc ctttgccgga ttaagcgcca actctccgtt ctggaactgt ttactggcgt
     2101 tctgcatgtt tgtcggtcgc ttcggggtga ttatcccggt gatggcaatt gccggttcgc
     2161 tggtgagtaa aaagagccaa cccgccagct ccggcacgct gccaacgcac ggcccgctgt
     2221 ttgttggcct gttaatcggc accgtgttgc tggttggcgc actgaccttt atccctgccc
     2281 tggcgcttgg tccggtggcg gaatatctct cctgatgata ttgagtgagc actgaatatg
     2341 agtcgtaaac aactggcgct attcgaacca acacttgtcg ttcaggcgct gaaagaagcg
     2401 gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc
     2461 ggcagtctgc tgaccacctg tattagcatc gcgatggcaa gcggtgcgat gcctggcaat
     2521 gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc
     2581 gccgaggcgc tggcagaagg ccgcagtaaa gcgcaggcca acagtcttaa aggggtgaaa
     2641 aaaactgcct ttgcccgcaa gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt
     2701 cctgccgatc aactgcgtaa aggtgacatc gtgctggtag aagctggcga tattatcccc
     2761 tgcgatggtg aagtcattga aggcggcgca tcggtcgatg aaagcgccat caccggggaa
     2821 tcggcaccgg tgatccgtga atccggcggc gattttgcct ccgttaccgg cggcacgcgt
     2881 attctttctg actggctggt gattgagtgt agcgttaacc ccggtgaggc atttctggat
     2941 cggatgatcg cgatggtgga aggcgcacag cgacgcaaaa caccgaacga gattgccctg
     3001 actatcctgc tgattgctct gaccatcgtc tttttactgg caaccgccac gctgtggccg
     3061 ttttccgcgt ggggcggtaa tgccgtgagt gtaacggtac tggtggcgct gctggtctgt
     3121 ctgatcccaa ccactattgg tggcctgttg tcagcgatcg gcgtcgccgg gatgagtcgg
     3181 atgctgggcg cgaatgtgat tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt
     3241 gacgttctgc tactggataa aaccggcacc atcacactcg gtaaccgtca ggcgtcggag
     3301 tttatccccg cgcagggcgt ggatgaaaaa acgctggctg acgccgcaca actggcttcg
     3361 ctggctgatg aaacgccgga aggtcgcagt attgtgatcc tcgccaagca acgttttaac
     3421 ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg taccgtttac tgcgcaaagc
     3481 cggatgagcg ggatcaacat cgacaaccgc atgatccgta aaggttctgt cgatgccatt
     3541 cgccgccata ttgaggctaa cggcggtcac ttccctgccg atgttgatca aaaagtcgat
     3601 caggttgcgc gtcaaggagc cacgctgctg gtggtggtgg aaggttctcg tgtgctgggc
     3661 gttattgcgc tgaaagatat cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgc
     3721 aaatgggcat taaaacggtg atgattaccg gcgataaccg tctgactgcc gccgcgatag
     3781 ctgcggaagc gggtgtcgat gattttctcg ccgaagcgac accggaggcc aagctggcat
     3841 tgatccgtca gtatcaggcg gaaggtcgtt tggtagcgat gaccggcgac agcaccaacg
     3901 atgctacggc gctggcgcag gcagatgtcg cggtggcgat gaactccggc acccaggcgg
     3961 ctaaagaggc gggcaatatg gtggatctcg actctaaccc gaccaagttg atcgaggtgg
     4021 tgcacattgg caaacagatg ctgatgaccc gtggctcgct gaccaccttc agcattgcca
     4081 acgatgtggc gaaatacttc gccattattc cggcggcgtt cgcggcaaca tatccgcagt
     4141 taaatgcgct gaacatcatg cgcctgcatt cgcccgactc cgccattctc agtgcggtca
     4201 ttttcaacgc cttgattatc gtctttttga ttcctctggc gttaaaaggc gtgagttata
     4261 aaccgcttac cgcttctgcc atgttgcgcc gtaacttatg gatttacggt ctgggtgggc
     4321 tgctggtgcc gtttattggt atcaaagtca ttgatttact gctaaccgtt tgtagtctgg
     4381 tgtgaggttt accatgagtg gattacgtcc ggcattatcg acatttctct ttctgttatt
     4441 gattactggc ggcgtttacc cgctgctgac caccgcactg gggcaatggt ggtatccctg
     4501 gcaggccaac ggttcgttga ttcgtgaagg tgatacggtg cgcggttcgg cattaatcgg
     4561 gcaaaatttt accggcaacg gctattttca tggtcgcccg tcggcaacag cagaaatgcc
     4621 ctataatcca caggcttctg gcgggagcaa tctggcagtc agtaacccgg agctggataa
     4681 acagatagcc gcacgcgttg ccgcattacg ggccgctaac ccggacgcca gcacgaacgt
     4741 tccggttgaa ctggtgacgg cgtcggcaag cggactggac aataacatca ccccgcaagc
     4801 ggcggcctgg cagatcccac gcatcgcaaa agcgcgtaat ctcagcgttg aacagctcac
     4861 gcagctgatc gcaaaataca gccaacaacc gctggtgaaa tatatcggcc agccggtcgt
     4921 caacattgtc gaactcaatc tggcactgga taagcttgat gaataacgaa cccttacgtc
     4981 ccgaccccga ccgtctgctg gaacaaactg ccgcgccgca tcgggggaag ctgaaagttt
     5041 tcttcagtgc ctgtgcaggc gtcgggaaga cctgggcgat gctggcagaa gcccagcgac
     5101 tgcgggcgca agggctggat attgtggttg gtgtggtaga aacccatggg cggaaagata
     5161 ccgccgccat gcttgaaggg ctggctgttc tgcctctgaa acgccaggcg taccgtgggc
     5221 ggcatatcag cgagtttgat ctcgatgccg ccctcgcccg ccgtccggcg ctgatcttaa
     5281 tggacgaact ggcgcacagt aatgcgccag gttctcgtca tcctaaacgc tggcaggata
     5341 tcgaagaact gctggaagct ggcattgatg ttttcacaac cgtcaacgtt cagcatctgg
     5401 aaagtctgaa tgatgtggtc agcggcgtca ccggaattca ggtacgggaa accgtgcccg
     5461 atcctttttt cgatgccgcc gacgacgtgg tgctggtgga cttgcccccg gacgatctgc
     5521 gccagcggct gaaagaaggc aaagtctata ttgccgggca ggcggagcgc gccattgaac
     5581 attttttccg caaaggcaat ctgatcgccc tgcgcgaact ggcactgcgc cgtactgccg
     5641 atcgcgttga tgagcaaatg cgcgcctggc gggggcatcc tggcgaagag aaagtctggc
     5701 acacgcgcga cgcgatcctt ttatgcatcg gccataacac cggcagcgaa aactggtccg
     5761 cgcggcggcg cggctgacgt cacggctggg gagcgtctgg cacgcggtgt atgttgaaac
     5821 ccctgccctg catcgcttac cggagaaaaa acgtcgggca attctcagcg ccttacgtct
     5881 ggcgcaggag ctgggcgcgg agacggcaac actttctgat ccagcggaag agaaagcggt
     5941 agtgcgttat gcccgtgaac ataatctcgg caagattatt ctcggtcgcc cagcctcgcg
     6001 ccgctggtgg cgtcgggaaa catttgctga ccgactggcg cgcatcgccc ccgatctcga
     6061 tcaggtgctg gtcgcgcttg atgaaccacc cgcccgcacg attaacaacg cgccggatag
     6121 ccgctcattt aaagacaagt ggcgggtaca gattcaggga tgcgtggttg ccgccgcgtt
     6181 atgcgccgtt atcaccttaa ttgccatgca gtggctgatg gcgtttgatg ccgccaacct
     6241 ggtgatgctg tatctgcttg gcgtggtggt ggcgctattt tatggacgct ggccttcggt
     6301 ggttgccacc gtcattaatg tggtgagttt cgatctcttt tttatcgccc cacgcggcac
     6361 gctcgccgtc tctgatgtgc aatatctgct gaccttcgcg gtgatgttaa ccgtcgggct
     6421 ggtgatcggg aaccttactg ctggtgtgcg ttatcaggcg cgggtagccc gttaccgcga
     6481 gcaacgcaca cggcacttat atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca
     6541 ggacatcgcc gccaccagcg aacaatttat tgcctccacg tttcatgccc gcagtcaggt
     6601 gttgttgccc gatgacaacg gtaaattgca gccgttaaca catccgcaag gaattacgcc
     6661 atgggacgat gccatcgcgc agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac
     6721 cgacacgtta cccggtgtac cgtaccagat tttgccgcta aaaagcggcg agaaaaccta
     6781 cgggctggtg gtggtggagc cggggaatct gcgccagttg atgatcccgg aacagcagcg
     6841 cctgctggag acttttacgc tgttagtcgc caatgccctt gagcggctga cgctaaccgc
     6901 cagcgaagaa caggcgcgga tggcaagcga acgtgaacag atccgcaacg ccctgctggc
     6961 agcgctttcg catgatttac gcacgccgct tacggtgctg tttggtcagg cagaaatctt
     7021 aacgctcgat ctggcaagcg aaggatcacc ccacgcccgc caggccagcg agattcgtca
     7081 gcatgtgctg aacactaccc gactggtgaa taatctactg gatatggcgc gaattcagtc
     7141 cggcggcttt aatttgaaga aagagtggtt aacgctggaa gaagtagtcg gcagcgcgct
     7201 gcaaatgccg gaaccgggtt tatcgtcgcc catcaatctt tctctgccag aaccgctgac
     7261 cttaatccac gttgacgggc cactctttga acgggtgctg attaatctgc tggagaacgc
     7321 ggtgaaatat gcgggtgcgc aggccgaaat tggtatcgat gcccacgttg agggcgaaaa
     7381 tctacaactg gatgtctggg ataacggccc cggtcttccg ccaggccagg agcagacgat
     7441 atttgataag tttgctcgcg ggaataaaga gtcggcggtg ccgggtgtcg ggcttgggtt
     7501 ggcaatttgt cgggcgattg tggatgtaca cggcggcacc attaccgcgt tcaaccgacc
     7561 ggaaggtggt gcctgttttc gtgttacact tccccagcaa accgctcctg aacttgaaga
     7621 ctttcatgag gatatgtgac aaacgttctg attgttgaag atgaacaggc tattcgtcgc
     7681 tttctgcgca cggcgctgga gggcgacggg atgcgcgtct ttgaggccga aactctgcaa
     7741 cgcggcttgc tggaagcggc aacccgtaag ccagatttga ttattctcga tctcggcctg
     7801 cccgatggtg atgggattga gtttatccgc gacctgcgcc agtggagccc ggtgccggtg
     7861 attgtgcttt ccgcacgcag cgaagagagc gacaagatcg ccgcgctgga tgccggagcg
     7921 gatgattatc tgagtaagcc gtttggtatt ggcgaattgc aggcccgtct gcgcgtcgct
     7981 ttacgccgcc actccgccac tccgccaccg ccgcgcccga tccgctggtg aaattttccg
     8041 gtgttaccgt cgatttagcc gcccgcgtga ttcaccgggg tgaggaagag gtgcatctca
     8101 caccaattga gttccgcctg ctggcggtgc tgctcaacaa tgccggaaaa gtactcaccc
     8161 agcgccagct ccttaaccag gtgtgggggc caaacgcggt cgaacacagt cactatttgc
     8221 gtatttatat gggacatctg cgacaaaaac tggaacagga tccggcccgg cctcgccact
     8281 tcattactga aaccggtatt gggtatcggt ttatgctttg aattgttatt caataaattc
     8341 gcttaaatag tgattaattt attattaaac ctgcgccgaa tattattatt tggcaggttt
     8401 aatttcttaa cgcaaatata aaacacgaaa ttacaacatt taagtaacaa aacagcatct
     8461 aatatggcga tattcataat taatgattta ttatctgggg tatgatcgtt ttttgttgat
     8521 cttcttcaca gattatagcc atttcatgga tagaataact ctaccttcaa ctgacacagc
     8581 aagcggtaaa ggtaaatgga aaataataac cgcttaatgc ctcatataag gcggacaacc
     8641 catatcatga agtttgccca tcgtaatagt tttgactttc atttctttaa tgcccgctag
     8701 tcttctgact aaagggcacc ccaacgtaca ggtctccctg actttaagca ttacaggtta
     8761 atacctgtat tcctcggtgc tcatatactg ctaacccttt taaactctaa ataattcgag
     8821 tcgcagcact tgcaacttga ggtatgacga gtatagccag ttaccgggct ggtctgggtt
     8881 attgcatctg caaaaagcaa actactgatt tatttatcag cggtggagct ttgctttttt
     8941 tccgacgtga tcgatttctc ctttgagaaa ttgaggacct gctattacct gaaataaaga
     9001 gatgaataaa atgtcagaat taaaaattgc cgttagccgt tcttgcccgg attgtttttc
     9061 cactcatcgt gcatgcgtga atattgacga aagtaattat attgacgttg ccgccattat
     9121 tttatcagtc aatgatgttg aacgtggaaa actcgataaa atagacgcta ctggctatgg
     9181 cattcctgtt tttattgcaa cggaaaatga agaacgtgta ccagcagaat atctgccacg
     9241 tatttctggt gtcttcgagc attgcgaatc acggaaagag ttttatggac gtcagttaga
     9301 aaccgctgct agccattatg aaacgcaact gcgcccgccc ttcttccgcg cactggtcga
     9361 ttatgtcaat cagggcaata gcgcttttga ctgcccgggt catcagggag gtgagttttt
     9421 ccgtcgtcat ccggcaggga atcagttcgt ggagtacttc ggagaaacgc tgttccgttc
     9481 cgacttatgc aacgcagacg tagcaatggg cgatctgctg attcacgaag gcgcgccatg
     9541 cattgcacag caacatgcgg caaaagtgtt taatgccgat aaaacatact tcgttctaaa
     9601 tggcacttca tcttctaaca aagtggtttt aaacgccctg ctaacaccgg gtgatctggt
     9661 gctgtttgat cgcaataacc acaaatctaa ccaccacgga gcgttgctac aggctggtgc
     9721 aacaccggtt tatctggaaa cggcacgtaa cccgtatggc tttatcggtg gcattgatgc
     9781 gcactgtttt gaagaaagtt acctgcgtga gctgatcgcg gaagtcgcac cgcagcgggc
     9841 aaaagaggct cgtcctttcc gcctcgctgt gattcagtta ggcacctacg acggtacgat
     9901 ttataacgcc cgccaggtgg tggataaaat tggtcatctg tgtgactaca ttctgtttga
     9961 ctccgcatgg gttggctatg agcaatttat cccgatgatg gctgactgtt cgccgctgtt
    10021 gctggatctt aatgagaacg atccgggtat tctagttacg caatccgtac ataaacagca
    10081 ggctggtttt tctcaaactt cacaaatcca taaaaaagac agccacatca aagggcaaca
    10141 gcgttatgta ccgcacaaac gcatgaacaa cgcctttatg atgcacgcct ccaccagccc
    10201 gttctatccg ctgtttgccg cactggatat caacgccaaa atgcatgaag gtgtcagcgg
    10261 tcgtaatatg tggatggatt gtgtggtaaa tggcattaat gcccgcaaac tgatcctcga
    10321 taactgtcag catattcgtc cgttcgtacc tgaactggtg gatggtaaac cctggcagtc
    10381 gtatgaaaca gcgcaaattg cggttgatct gcgcttcttc cagtttgtac caggggaaca
    10441 ctggcattct tttgaaggct atgcagagaa tcaatacttt gtcgatcctt gcaaactgtt
    10501 gctgacaacc ccaggtattg atgcacgtaa cggcgaatat gaagcgttcg gtgtacccgc
    10561 gacgattctt gctaacttcc tgcgcgaaaa tggcgtagtg ccggaaaaat gcgatcttaa
    10621 cgccatcctc ttcctgctga ctccggcaga agatatggcc aaacttcagc aacttgtgcc
    10681 ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga agtgctgcct
    10741 tccatctaca aacagcatga agagcgctac gccggttata ccctgcgtca gttgtgccag
    10801 gaaatgcatg atttgtatgc ccgccacaac gtgaaacaac tgcaaaaaga gatgttccgc
    10861 aaagcgcact tcccacacgt cagcatgaat ccgcaagaag ccaactacgc ctatttacgc
    10921 ggtgaagtgg agctggttcg tctaccggat gccgaaggtc gtatcgctgc cgaaggggcg
    10981 cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg tggtgctgtt
    11041 ttgcgttact tcagcgctct ggaagaaggg atcaacctgc tgccaggttt tgcaccggag
    11101 ctgcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg ctatgtcatc
    11161 aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt caggctaaat
    11221 cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg atgggctccg
    11281 gtatcatcat gctgccgaca aagcttgccg aagttgggac aatttcaatt atctcctggc
    11341 tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc ggtatgttca
    11401 gccgtaaatc aggtggtatg ggcggttatg ccgagtatgc tttcggtaaa tccggtaact
    11461 ttattgcgaa ctatacctac ggcgtctcac tgctgattgc taacgtcgcg attgctattt
    11521 cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg cagattggtc
    11581 ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt gcgcgcatta
    11641 ccgggcaaat cagtagcatt accgtgtggg ggggcattat tccggtggta ggcctgtgca
    11701 ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat ccgcatcatg
    11761 caccgttctt cagtgcagta ggttcttcca tcgctatgac gctgtgggct tttcttggtc
    11821 tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat gtgccaatcg
    11881 cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc aacgtgattg
    11941 cagggattgt gcctaatatg gagctggcaa attcaacggc accatttggc ctggccttcg
    12001 cacagatgtt tacaccagaa gtgggtaaag tcattatggc gctgatggtg atgtcctgct
    12061 gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaaatct tcatctgatg
    12121 aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg gtgcagggaa
    12181 tgttgaccat tgtgattatc cagagcgggt tggcgctgat gacgattagc ccgtcgctaa
    12241 acagtcagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatt ccgtatattc
    12301 tgtcaatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc tcaaaagcga
    12361 aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg ctgtactcat
    12421 ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg acactgtatg
    12481 gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc aaagtaaaaa
    12541 agggcgatct tgcgaccgct ctttttttat taaatgtgtt tacgcgtttt tcagaacttc
    12601 gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac cgaggaagct
    12661 ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga accagccgtt
    12721 gtcagtcatc actttcagac cgccaataga agcaccgttg cccggagcag cagtcaggcg
    12781 cgcggtgatc gggtcacctg ccagggtgct ggcgctcacc atttccggag acagcttaga
    12841 cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc tcggcgcacc
    12901 aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga cagcggtgat
    12961 ttccgccgcc agcagacaca taatgatgcc gtctttgtcg gtggaccacg gcgtgccgtc
    13021 gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc tgccgtcgaa
    13081 cagaccatca acaaaccatt tgaaacctac cggtacttct accagcttac ggcccaagtc
    13141 attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag caacatcttt
    13201 gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt agtggttcgg
    13261 attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt cgttagcaaa
    13321 cgccagatcg aacttatcac gcagtgccag cagacccgcc atcgcacact cggaggagca
    13381 gtccatacgg atcgcaccgt ctttatcgag gtgcataaag cggaaggttt gatcgacctg
    13441 atcgttaacg atggtcaggt tgaggttgta atactcgcca atgcgcttcc agtattcgat
    13501 accggaaccg cccaacggat caacgcccag cgtcaggccc gctttctgaa tcgccgccat
    13561 atcaacgata tcggccagcc cttccacgaa cggctgcacc agatcctgct ctttcacata
    13621 gccggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc catcggccat
    13681 cagtgcgttg gccttgtctt ccaccacttt ggtgacgtta gtatcagccg ggccaccatt
    13741 tggcggattg tacttgatac caccatcttc cggcgggtta tgggacggtg taatcacgat
    13801 accgtctgcc agcgggccac cttttttatt gtgaaccagg atggcattgg aaacggcagg
    13861 cgtcggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg ctgccagcac
    13921 ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac ccacatagca
    13981 agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga tcgccagaat
    14041 gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag taccgaattt
    14101 caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg ccgtcagttg
    14161 ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt ggattgccat
    14221 tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca atcaattccg
    14281 ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg ccggtgttgg
    14341 tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc tgattaccat
    14401 ttttcagcag cgtttgttca tctgccgcaa agtacacgcg tgtacgaccg tgcaacgatt
    14461 ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac agcagcataa
    14521 agcgattgac cgctcgcttt tgctctgcgt attcgtccga aagcagaagt tcacgcattg
    14581 cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca ggtgacgcaa
    14641 cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat ttcaacatac
    14701 gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca atatagctgt
    14761 agagttcatc atcaacttca atcgttttca tcttaatcca gtgcagtgtc ttagctgaat
    14821 acaagtcgcc aagagtatat gaataaatcc cggcagcgga tagcgtcaag cctggcttgc
    14881 agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt caacataata
    14941 ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attaaatatc cgcgcgcaaa
    15001 ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg tttggtagcc
    15061 tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc atccaggttg
    15121 atatgcgtaa ccacggtctt tcaccgagag agccggtaat gaattacccg gcgatggcgc
    15181 aggatcttgt tgataccctg gatgcactgc agatcgacaa agcaacattt atcggtcact
    15241 ccatgggcgg taaagcggta atggcactta ctgcactggc ccccgatcgc atcgataaac
    15301 tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat gagattttcg
    15361 cagctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca gcagcaataa
    15421 tgcgccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct tttgttgacg
    15481 gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt gtgggttggg
    15541 agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat tctccgtatg
    15601 ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg gcgcatgtga
    15661 ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta cgcgctatcc
    15721 gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt cgctttccgc
    15781 gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc catccgggca
    15841 gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga acaaacggac
    15901 cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa aactaatccg
    15961 ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg cgacaaagta
    16021 cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc gctgaacgag
    16081 ctggcggaat cgcgtaatat gagccgtagc gaactgatcg aagagatgtt aatgcagcaa
    16141 ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta gcacagtgtg
    16201 cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt gcctttatcc
    16261 gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac tcatggctat
    16321 cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa aaatgattca
    16381 aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca gcaaagaaga
    16441 tctggaagct tacgacattc tgctgctggg catcccaacc tggtattacg gcgaagcgca
    16501 gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaatg gtaaactggt
    16561 tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg acgcattggg
    16621 caccatccgc gacatcattg aaccgcgcgg tgcaaccatc gttggtcact ggccaactgc
    16681 gggctatcat ttcgaagcat caaaaggtct ggcagatgac gaccactttg tcggtctggc
    16741 tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat gggttaaaca
    16801 gatttctgaa gagttgcatc tcgacgaaat tcttaatgcc tgatgtgatg cggcgtagac
    16861 tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca aatttgtaac
    16921 ttttgctgtt gtacctatac aatgtcccag tgttcaagtg gccttgccgt tgtaaatgta
    16981 agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc atttaggcgt
    17041 ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc ttctaatgaa
    17101 gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg ccctaaagaa
    17161 agctggcctg aaagtaacgc ttcctcgttt aaaaatcctg gaagttcttc aggagccgga
    17221 caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg gtgaagaaat
    17281 tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta tcgtcacccg
    17341 ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc accacgatca
    17401 cctgatttgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca tcgaagcgcg
    17461 tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc tctatcttta
    17521 cggtcactgt gccgaaggcg attgccgcga agatgagcac gcgcacgaag gcaaataagc
    17581 cagcctgaac gagaaaagcc aacctgcggg ttggcttttt tatacaaggg aaataagaaa
    17641 agcgagagtt acagctctca cttatttgtt attactgcgt atttcctgcc agaccttatc
    17701 acaatcggct ttcaccgcct gatcatttcc ggtttgcgtt gcacgcagac actgctgata
    17761 atccagtacg cgtacacttt cctggtcggc aaactgctga tgttgtttct ctttcttcag
    17821 cacgtttaac acgctctggc aggcttcaat tttttctggc gaaccttgcg cggtgttgat
    17881 acaggcgctg tatgcctctt tcagacggct gtcttcctgt ggtgcggttg attgcgcaca
    17941 agctaccagc cccgatgcca tcatggcgat gagaatcagt tttttcattg aatgcggcag
    18001 gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat ggtgcgatta ccataaattt
    18061 cacgtcacgc tcatcctgga agatgttacc gtaaccgccg ccccagcttg ggatgtcgga
    18121 gtggttgtcg tattcggtga agtgcagttt gaacatcgtg cctttggcgc gaccgtcctg
    18181 aatggtgtag accgcatcca ggctgtaggc agactcttca atagtccggt ttttgtcgta
    18241 gtacgcatcc ggattgctct gccaggtcgc aggtttagca tcccatgcgt aaacgtagga
    18301 agcgccgatg gcgaagcctg gaagattcca gtttttcagg tcatacatcg cactgaagaa
    18361 gaccgctttt tcgccgttag cgttgaagtc ggaacggtta tcccaccaga tatccaggcg
    18421 gccgttggag gaagcgtagg ttggggtcat acgttgcagg aagtatccct gctgactgtc
    18481 agccttaatc caggtgcctt cgaggcgcaa atccactacg tcagccgccc ggtaaccaaa
    18541 ggtcaacgct tgcagccagg cggtgccgtc ataaagatcg ttgacgctgc gatcgtcaac
    18601 tttatcgcgg gtaccataga actggtagct ggtggttaac gggctaccgg cgatatcaaa
    18661 tttgtagctg gctttggcaa aatattgatc gatataccct tccgcctgac caaatgccgc
    18721 ttccagtacg aagttatttt tgaagtcgta tttcgcaccg atggagtgca gataatcaac
    18781 tttggtggtt ttatcgttct gataaaactc atccatttcc agatgccacg gcgctttgta
    18841 ttcgttggtc cacatgtagg agaaactcaa ggcaccagca tcgccgtaat caaaattcgc
    18901 cccggcttcc gccccctgat aagtacccgg cataaagctc cagtgcggcg ctaacagcgt
    18961 ttgaccggtt ggctgaatgt aacctgccct cgcccaaacc ggaccgtatt taaatttggc
    19021 cgcagcttta tacaggctta taccgctttt gtcgccggac cagtcttcgt cataggcttt
    19081 attacttttt gaaaacgcga tttcgttcgg gtgggagctg tcgccgtttt ccgccatttc
    19141 aatcgccgta aacgcagcaa tatcaaggcc gaacatatca gcagcataac cggactgaaa
    19201 atcgaggttg gcattccagg tggagtgaga aaggttggtt ttgtatttgt cgccgtcggt
    19261 aacatcttta cggtcgcgtt cacgctgcca gtaatagata ccgccggtta aggttgaatc
    19321 gtcgatgaat ccttcggcgc gagcctccgg cattgccata aagcccgaca ttgctgtaac
    19381 accggcaata gccagcgcca gcgtactacg tttgccacta aacgtacgca tgggttaatc
    19441 ctctttgacg tataaattgc tgcaccaaag gtgaacagcg aaaaaaaata aaaattaaaa
    19501 aagtagcgtt gagaaaagtc tcgctgacta caggaacaga ctatttttcg cgacgcgaat
    19561 tatcaatctc tttctgtaac cagaatgtaa gaacatgagc aaccgcacat attttgatga
    19621 tttttgttac aaggcttaag tttgcggttg aaaggcttgc agaagaataa aaacgtgaaa
    19681 ttccagcaag atgaaagcgg ttacatttta tttaattatg ttttgtgatt ttaaatttat
    19741 tttatggata aattgcgaag cggaattaat tcgcgaagaa aagcagaaaa aacgccgcag
    19801 agcgcggcgt ttgggattgt ttgctatttc ttgccggatg cggcgtgaac gccttatccg
    19861 gcctacaaaa gcacgcaaat tcaatatatt gcagagatca tgtaggcctg ataagcgaag
    19921 cgcatcaggc aatttagcgt ttgaaattta ttgtctattt attgccggat gcgacgtaaa
    19981 tgcctcaccc ggcttggtag aacttaaaat tactcgccta ctttcgccca ggtatcacgc
    20041 agcccaacgg tgcggttaaa taccggtttt tcggccgtgg aatggcggct gtcgaggcag
    20101 aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa ccgcatcttt cagcgacggt
    20161 tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag gaaatcatcc
    20221 gcagcacctg ggttaggcac gctgaacaga cgatcataca aacggatttc aaccggcagc
    20281 gcatgtgccg cgctcaccca gtgaataaca cctttgactt tacgaccatc tgccggatct
    20341 ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg tgatattacc ttcggcatct
    20401 ttctccacgc gttcagcctt aatgacgtaa gcattacgca gacgcacttc tttacccagc
    20461 accagacgtt tgtactgctt gttagcttct tcgcggaaat cggcgcgatc gatccaaatt
    20521 tcaccgctaa acggcacctg acggctcccc atttccggtt tgttcggatg gttcggcatg
    20581 gtgaccattt cgccttcgcc ctgatagttt tcgataacca gtttcaccgg atcgataacc
    20641 gccattgcgc gcggcgcatt ttcgttgaga tcttcacgga tgcaggattc cagcgacgcc
    20701 atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt tgcagaactc acgaatagaa
    20761 gccgcagtgt aaccacgacg acgcagaccg gaaatggtcg gcatacgcgg gtcatcccag
    20821 ccttcaacgt gcttgtcggt caccagcagg ttcagcttac gcttggacat cacagtgtat
    20881 tccagattca ggcgcgagaa ttcgtactgg cgcggatgaa caggaatggt gatgttatcc
    20941 agtacccagt cgtacagacg acggttgtcc tggaactcaa gcgtacacag agagtgcgta
    21001 ataccttcca gcgcatcgct gatgcaatgg gtgaagtcgt acatcgggta gatgcaccac
    21061 ttgttgccag tctggtggtg ttcagcaaat ttaatgcggt acagcaccgg atcgcgcatt
    21121 acgataaatg gcgacgccat gtcgattttc gcacgcaggc aggctttacc ttcttcaaaa
    21181 ccaccggcac gcattttttc gaacagcgcc aagttctctt caacgctgcg gtcgcggtac
    21241 gggctgtttt tacccggctg cgtcagggta ccgcggtatt cgcggatctg ttccggtgtc
    21301 agttcatcaa cgtacgccag gcctttattg atcagttcga tcgcataggc gtggagctga
    21361 tcaaaataat cggaggagta acggacgtta ccagaccagt gaaaacctaa ccactctacg
    21421 tcgtttttga tcgaatcgac atactcgata tcttctttta ccgggttagt gtcgtcgaaa
    21481 cgcaggttgc actggccttt atagtcctgg gcgatcccga agttcaggca gatagatttc
    21541 gcatggccaa tatgcagata gccattcggc tccggcggga aacgggtgtg tactgtgatg
    21601 tgcttaccac tggccagatc ttcatcgatg atctgacgga taaagttagt cgggcgggct
    21661 tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac gtataacggc gtatgatctt
    21721 ataagcggga caggctgaca actgttagtt ttttaaaatt gataatctaa caaggattat
    21781 ggggatgatt gatgaaaaaa aaagcggcag agaagatctc caccgcttgt cattgttgga
    21841 tgcgacgctg ccgtgtcgca tcaggcataa agcagattac tttttgattt catacagcgg
    21901 tgtttgaccc gccacaacat ggccctgagc tttaatgagc aagccactga aatcgtcgat
    21961 attgctgcaa accaccgggc taatcatcga gcgggcgtta gcgttcaggt aatccagatc
    22021 catttccaga atcggttgcc ctgcgcttac ctgcgcaccc tcttccacca gacgtttaaa
    22081 gcctttacct tccagcgcta cggtgtcgat acccatatgg acgacgatct ccgcgccttt
    22141 ttcggtttcc aggcagaacg cgtggttggt gttgaagatt ttcacgattg tccctgcggc
    22201 tggtgatacg acgattttat ctgtcggttt caccgccaca ccgtcaccca ccgctttgct
    22261 ggcgaatgct tcgtcaggaa cctgatccag tgccacgaca tcaccggtaa tcggcgatac
    22321 cagctccgcg atagataccg cgtttggtac agcctgcggt tttgctacag gcgcggcagt
    22381 tgccggagtt gcttcagctg acgcagcggc taccggacca cgggcaacga ctttcttcat
    22441 cgcatcgccg atggattctg ctttcgcgcc aacaatcacc tgaatagtct gtttgttcag
    22501 tttcactaca ccagaagcac ccagacgttt acacatcgta tcgttgacgc gggcagagtc
    22561 agccacggtc aggcgcagac gggtaataca ggcatcaatc gctttcaggt tgtcagtacc
    22621 gccaaccgca gcaatatagt tggttgccag ttgagtcaga ccttcttcag tattgctgtt
    22681 ggcttcttca gtaacgatct cgtcttcttt atcttcacga cccggcgttt tcaggttgaa
    22741 catgcggata accaaactga acaccacgaa gtagatagcg aagaagataa cgcccatcac
    22801 cagcagcatc cagacgttct ggctggcggc aggcaggtta tacatcaacg cgtagtcgat
    22861 agcacccgca gagaaagaga agccggcatg gatacccagc agcgttgcca caaacaggct
    22921 gataccggtc agcagtgcgt gcaggaggta cagcagcggc gcaaggaaca tgaacaggaa
    22981 ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca gcaacagaaa gcagcatccc
    23041 gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac atcgccagcg ccgcacccgg
    23101 cagaccgaac atcatgatcg ggaagaagcc ggacatgaac atccccgcgg tgccgtcacc
    23161 agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc gccgcgttgg tgaattcacc
    23221 aatctggaac caggcgatgg tgttcagcac ctgatgcaga ccggttggga tcagcagacg
    23281 gttgatgaaa ccaaagatac cggaacccag cgcgcccgca gaaacgatcc actcgccgcc
    23341 tgcatggata gcgtgctgta ccggcggcca gacgtaacca aaaatggccg ccagcaccag
    23401 acagaagaag ccggtggcga tcggcacaaa gcgtttgccg ccgaagaagc tcaggaagtc
    23461 cggcagttta atatcggacc aacggttata ggctgcgcca ccaaccagac cggtaatgat
    23521 acccgccagt acacccatgt taatttctgg gttgatggtc accatcgctt tggttaacac
    23581 aaagtaacct accgcacccg ccagcgccgc cgcacctgcg ctgtctttcg accagctgga
    23641 tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa attgcaccgc ccgcctgggc
    23701 aataaacgca acgttaagta aatctggctg accgaatcgc agcaacagcg ccgccaccgg
    23761 cagcaccgcg atagggagct gtaacgccct accgagtcgc tggaaaaaac ctaaaatatt
    23821 catcttattc cccctacgag aaccctattt ggctcgttta aagccgtatt tttattttgc
    23881 tgcaaatttt actgccgatg ttctgtaatc agattgttag atcatctgct acagagtgtg
    23941 tgaaaattta attcgcatcg caaattaaac gcgtgtcttt tgtgagtttt gtcaccaaat
    24001 atcgttatca tcactccctt ttactggcta aaccagaaaa cttattttat cattcaaaaa
    24061 atcaagtcgg attgacgcct gtctgcgcaa atccaggtta cgcttaaaga tgtctaatcc
    24121 gccaacggct tacattttac ttattgaggt gaataatgag actgatcccc ctgactaccg
    24181 ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa tcgtatcaat gcgttcaaac
    24241 caactgccga tcgtccgttt gtactgggcc tgccgactgg cggcacgccg atgaccacct
    24301 ataaagcgtt agtcgaaatg cataaagcag gccaggtcag ctttaagcac gttgtcacct
    24361 tcaacatgga cgaatatgtc ggtctgccga aagagcatcc ggaaagctac tacagcttta
    24421 tgcaccgtaa tttcttcgat cacgttgata ttccagcaga aaacatcaac cttctcaacg
    24481 gcaatgcccc ggatatcgat gccgagtgcc gccagtatga agaaaaaatc cgttcttacg
    24541 gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg tcatattgca tttaacgaac
    24601 cggcgtcttc tctggcttct cgtactcgta tcaaaaccct gactcatgac actcgcgtcg
    24661 cgaactctcg tttctttgat aacgatgtca atcaggtgcc aaaatatgcc ctgactgtcg
    24721 gtgttggtac actgctggat gccgaagaag tgatgattat ggtgctgggt agccagaaag
    24781 cactggcgct gcaggccgcc gttgaaggtt gcgtgaacca tatgtggacc atcagctgtc
    24841 tgcaactgca tccgaaagcg atcatggtgt gcgatgaacc ttccaccatg gagctgaaag
    24901 ttaagacttt aagatatttc aatgaattag aagcagaaaa tatcaaaggt ctgtaattgt
    24961 tatccctgcc ctggttcctt gctcagggca atatttttta aaatcggggg gcagaatgta
    25021 tgcattaacc cagggccgga tctttaccgg ccacgaattt cttgatgacc acgcggttgt
    25081 tatcgctgat ggcctgatta aaagcgtctg tccggtagcg gaactgccgc cagagatcga
    25141 acaacgttca ctgaacgggg ccattctctc ccccggtttt atcgatgtgc agttaaacgg
    25201 ctgcggcggc gtacagttta acgacaccgc tgaagcggtc agcgtggaaa cgctggaaat
    25261 catgcagaaa gccaatgaga aatcaggctg tactaactat ctgccgacgc ttatcaccac
    25321 cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgt gagtacctgg caaaacatcc
    25381 gaatcaggcg ttaggtctgc atctggaagg tccgtggctg aatctggtaa aaaaaggcac
    25441 ccataatccg aattttgtgc gtaagcctga tgccgcgctg gtcgatttcc tgtgtgaaaa
    25501 cgccgacgtc attaccaaag tgaccctggc accggaaatg gttcctgcgg aagtcatcag
    25561 caaactggca aatgccggga ttgtggtttc tgccggtcac tccaacgcga cgttgaaaga
    25621 agcaaaagcc ggtttccgcg cggggattac ctttgccacc catctgtaca acgcgatgcc
    25681 gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc ctcgacgaag ctgacattta
    25741 ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc aacattcgta acgctaaacg
    25801 ccttaaaggc gacaaactgt gtctggttac cgacgccacc gcgccagcag gtgccaacat
    25861 tgaacagttc atttttgcgg gtaaaacaat atactaccgt aacggacttt gtgtggatga
    25921 gaacggtacg ttaagcggtt catccttaac catgattgaa ggcgtgcgta atctggtcga
    25981 acattgcggt atcgcactgg atgaagtgct gcgtatggcg acgctctatc cggcgcgtgc
    26041 gattgacgtt gaaaaacgtc tcggcacgct cgccgcaggt aaagtagcca acctgaccgc
    26101 attcacacct gattttaaaa tcaccaagac catcgttaac ggtaacgagg tcgtaactca
    26161 ataagagaaa gtatgacacc aggcggacaa gctcagatag gtaatgttga tctcgtaaaa
    26221 cagcttaaca gcgcggcggt ttatcgcctg attgaccagt acgggccaat ctcgcggatt
    26281 cagattgccg agcaaagcca gcttgccccc gccagcgtaa ccaaaattac gcgtcagctt
    26341 atcgaacgcg ggctgatcaa agaagttgat cagcaggcct ccaccggggg ccgccgcgct
    26401 atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg gcgtacggct tggtcgtcat
    26461 gacgccacca tcactctgtt tgatctcagc agcaaagtgc tggcagaaga acattacccg
    26521 ctgccggaac gtacccagca gacgctggaa catgccctgc tgaatgccat tgctcagttt
    26581 attgatagct accagcgcaa gctgcgcgag ctaatcgcca tttcggtgat cctgccaggg
    26641 cttgttgacc cggacagcgg caaaattcat tacatgccgc atattcaggt agaaaactgg
    26701 gggctggtag aagcactgga agaacgtttt aaagtgacct gtttcgttgg tcacgatatc
    26761 cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc aggattgcga agactccatt
    26821 ctggtgcgtg tccatcgcgg aaccggggcc gggattatct ctaacgggcg catttttatt
    26881 ggccgcaacg gtaacgtcgg tgaaattggc catattcagg tcgaaccgct gggtgaacgc
    26941 tgccactgcg gcaactttgg ctgcctggaa actatcgccg ccaacgctgc cattgaacaa
    27001 cgggtgttga atctgttaaa gcagggctac cagagccgtg tgccactgga cgactgcacc
    27061 atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagcc tggcctcgga agtgattgag
    27121 tatgtcggtc gtcatctggg taaaaccatc gccattgcta tcaacttatt taatccgcaa
    27181 aaaattgtta ttgctggtga aatcaccgaa gccgataaag tgctgctccc tgctattgaa
    27241 agctgcatta atacccaagc gctgaaggcg tttcgtacta atctgccggt ggtacgttct
    27301 gagctggatc accgctcggc aatcggcgct tttgcgctgg taaaacgcgc catgctcaac
    27361 ggtattttgc tccagcattt gctggaaaat taatgtgctt ttatagtggc gcttattgtt
    27421 gtcaatattc tgggtagtcc atgaccatta aaaatgtaat ttgcgatatc gacggcgtgc
    27481 tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt tttgcacggg attatggata
    27541 aaggcctgcc gctggtgttg ctgaccaact atccttcgca gactgggcaa gatctggcga
    27601 accgctttgc cactgccggt gtcgatgtgc cggatagcgt gttttatacc tctgcgatgg
    27661 cgaccgccga tttcctgcgt cgctaggaag gcaagaaagc gtatgtggtg ggcgaaggcg
    27721 cactgattca tgaactgtac aaagccggtt tcactattac cgatgtgaac cctgatttcg
    27781 tgattgttgg cgaaacacgt tcctacaact gggacatgat tcataaagca gcctatttcg
    27841 tcgctaacgg tgcacgcttt atcgccacca acccggacac ccacgggcgc ggtttttatc
    27901 ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc cgggcgcaaa ccgttctatg
    27961 ttggtaagcc cagcccgtgg atcatccgcg cggcattaaa caaaatgcag gcgcattcgg
    28021 aagaaacagt gattgtcggc gataacctgc gtaccgatat tctggcgggc ttccaggccg
    28081 gtctggagac gattctggtg ctttctggtg tttcatcgct cgacgatatc gacagtatgc
    28141 cgttccgccc aagctggatt tacccgtcag tcgctgaaat cgacgttatc tgaataccac
    28201 ccacgtctgc ggacgtggtt ggctctgatg acaaacgcca aactgcctga tgcgctacgc
    28261 ttatcaggcc tacgcagctc ctgcaatata ttgaattttc atgcttttgt aggccggata
    28321 aggcgttcac gccgcatccg gcatgaacaa atcgcacttt atcagcaatt tggcccccgt
    28381 aaaatataac cattcaataa aacccgctaa aaattatcga ttcatcaata aatacatcct
    28441 cacaccagac cctttttcac caatcgacaa tcaccattac gttttttatt tttgcactgc
    28501 caaatagctt gcgcacagtg ctgctttgcg gcattttttt aaacaagcaa acacaacaag
    28561 caacaaatac caggttaacg gagaaggtta tgtgttcaat ttttggcgta ttcgatatca
    28621 aaacagacgc agttgagctg cgtaagaaag cactcgagct gtcacgcctg atgcgtcatc
    28681 gtggcccgga ctggtccggt atttatgcca gcgataacgc cattctcgct cacgaacgtc
    28741 tgtcaattgt tgacgttaac gcgggggcgc aacctctcta caaccaacaa aaaaaccacg
    28801 tactggcggt aaacggtgaa atctacaacc accaggcatt gcgcgctgaa tatggcgatc
    28861 gttatcagtt ccagacaggg tctgactgtg aagtgatcct cgcgctgtat caggaaaaag
    28921 ggccggaatt tctcgacgac ttgcagggca tgtttgcctt tgccctgtac gacagcgaaa
    28981 aagatgccta cctgattggt cgcgaccatc tggggatcat cccactgtat atggggtatg
    29041 acgaacacgg tcagctgtat gtggcctcag aaatgaaatc gctggtgcca gtttgccgca
    29101 cgattaaaga gttcccggcg gggagctatt tgtggagtca ggacggcgaa atccgttctt
    29161 attatcatcg cgactggttc gactttgatg cagtgaaaga taacgtgacc gacaaaaacg
    29221 agctgcgtca ggcactggaa gattccgtta aaagccatct gatgtctgat gtgccttacg
    29281 gtgtgctact ttctggtggt ctggattcct caattatttc cgctatcacc aagaaatacg
    29341 cagcccgtcg cgtggaagat caggaacgct ctgaagcctg gtggccgcag ttacactcct
    29401 ttgctgtagg tctgccgggt tcaccggatc ttaaagcagc ccaggaagtg gcaaaccatc
    29461 tgggcacggt gcatcacgaa attcacttca ctgtacagga aggtctggat gccatccgcg
    29521 acgtgattta ccacatcgaa acttatgatg tgaccactat tcgcgcttca acaccgatgt
    29581 atttaatgtc gcgtaagatc aaggcgatgg gcattaaaat ggtgctgtcc ggtgaaggtt
    29641 ctgatgaagt gtttggcggt tatctttact tccataaagc gcccaacgtt aaagaactgc
    29701 atgaagagac ggtgcgtaaa ctgctggcct tgcatatgta tgactgcgcg cgcgccaaca
    29761 aagcgatgtc agcctggggc gtggaagcac gcgttccgtt cctcgacaaa aaattccttg
    29821 atgtggcgat gcgcattaac ccgcaggata aaatgtgcgg taacggcaaa atggaaaaac
    29881 acatcctgcg tgaatgtttt gagtcatacc tgcccgcaag cgtggcctgg cggcagaaag
    29941 agcagttctc cgatggcgtc ggttacagtt ggatcgacac cctgaaagaa gtggcggcgc
    30001 agcaggtttc tgatcagcaa ctggaaactg cccgcttccg cttcccgtac aacacgccga
    30061 cctcaaaaga agcgtatctg taccgcgaga tctttgaaga actgttccca cttccgagtg
    30121 ccgctgagtg cgtgccgggc ggtccttccg tcgcgtgttc ttcagctaaa gcgatcgaat
    30181 gggatgaagc gttcaaaaaa atggacgatc cgtctggtcg tgcggttggc gttcaccagt
    30241 cggcgtataa gtaagaaatg cggtactgcc ccggagaaat ccggggctgt tttagttatt
    30301 gcgctgcttg ttgattctga tatgcagtaa ggaaatgttc cgggtttgcc acatcactgt
    30361 ttgacaactc atcactgtag gccagtattt catagattaa tttcccacca ctattgttga
    30421 attccgaatt tcatcctgta cgacaaaaga atggctgtag ggtggggaaa aacacaaagt
    30481 aaacaataat tgacgaatat agcgccacgc tgttcgcaac ctaaccaaac agtcactttc
    30541 gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat aatgcgcccc
    30601 gcaacgccga taaggtatcg cgaaaaaaag
//
LOCUS       DAJTRN010000042        30257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRN010000042 DAJTRN010000000
VERSION     DAJTRN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1189
                     /locus_tag="OO328_001974"
     CDS             <1..1189
                     /locus_tag="OO328_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5930553.1"
                     /translation="RLRDARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLS
                     DGQIRQGLSAMVQLLEHYFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGG
                     RSYRVILLGLFATLLQAKGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQ
                     RIATTNSGELLSLTPVEHVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSS
                     LFARCWLLVEGETETWVINELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEW
                     HVLVDGDEAGKKYAATVRSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQ
                     IPENVPMNLRKIISKAIHRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARG
                     RAD"
     gene            complement(1186..2142)
                     /locus_tag="OO328_001975"
     CDS             complement(1186..2142)
                     /locus_tag="OO328_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5930554.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2293..3408
                     /gene="macA"
                     /locus_tag="OO328_001976"
     CDS             2293..3408
                     /gene="macA"
                     /locus_tag="OO328_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5930555.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3405..5351
                     /gene="macB"
                     /locus_tag="OO328_001977"
     CDS             3405..5351
                     /gene="macB"
                     /locus_tag="OO328_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5930556.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5424..5648)
                     /gene="cspD"
                     /locus_tag="OO328_001978"
     CDS             complement(5424..5648)
                     /gene="cspD"
                     /locus_tag="OO328_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5930557.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            5971..6291
                     /gene="clpS"
                     /locus_tag="OO328_001979"
     CDS             5971..6291
                     /gene="clpS"
                     /locus_tag="OO328_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5930558.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6322..8598
                     /gene="clpA"
                     /locus_tag="OO328_001980"
     CDS             6322..8598
                     /gene="clpA"
                     /locus_tag="OO328_001980"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5930559.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9001..9088)
                     /locus_tag="OO328_001981"
     tRNA            complement(9001..9088)
                     /locus_tag="OO328_001981"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9052..9054),aa:Ser,seq:gga)
     gene            complement(9342..9560)
                     /gene="infA"
                     /locus_tag="OO328_001982"
     CDS             complement(9342..9560)
                     /gene="infA"
                     /locus_tag="OO328_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5930560.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(9845..10549)
                     /gene="aat"
                     /locus_tag="OO328_001983"
     CDS             complement(9845..10549)
                     /gene="aat"
                     /locus_tag="OO328_001983"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5930561.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(10591..12312)
                     /gene="cydC"
                     /locus_tag="OO328_001984"
     CDS             complement(10591..12312)
                     /gene="cydC"
                     /locus_tag="OO328_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5930562.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12313..14079)
                     /gene="cydD"
                     /locus_tag="OO328_001985"
     CDS             complement(12313..14079)
                     /gene="cydD"
                     /locus_tag="OO328_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5930563.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14202..15167)
                     /gene="trxB"
                     /locus_tag="OO328_001986"
     CDS             complement(14202..15167)
                     /gene="trxB"
                     /locus_tag="OO328_001986"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5930564.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            15711..16205
                     /gene="lrp"
                     /locus_tag="OO328_001987"
     CDS             15711..16205
                     /gene="lrp"
                     /locus_tag="OO328_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5930565.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16340..20368
                     /gene="ftsK"
                     /locus_tag="OO328_001988"
     CDS             16340..20368
                     /gene="ftsK"
                     /locus_tag="OO328_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5930566.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            20523..21134
                     /gene="lolA"
                     /locus_tag="OO328_001989"
     CDS             20523..21134
                     /gene="lolA"
                     /locus_tag="OO328_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5930567.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21145..22488
                     /gene="rarA"
                     /locus_tag="OO328_001990"
                     /gene_synonym="mgsA"
     CDS             21145..22488
                     /gene="rarA"
                     /locus_tag="OO328_001990"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5930568.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            22579..23871
                     /gene="serS"
                     /locus_tag="OO328_001991"
     CDS             22579..23871
                     /gene="serS"
                     /locus_tag="OO328_001991"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5930569.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24111..26554
                     /gene="dmsA"
                     /locus_tag="OO328_001992"
                     /pseudo
     CDS             24111..26554
                     /gene="dmsA"
                     /locus_tag="OO328_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            26565..27182
                     /gene="dmsB"
                     /locus_tag="OO328_001993"
     CDS             26565..27182
                     /gene="dmsB"
                     /locus_tag="OO328_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5930570.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27184..27975
                     /locus_tag="OO328_001994"
     CDS             27184..27975
                     /locus_tag="OO328_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5930571.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28011..28637)
                     /locus_tag="OO328_001995"
     CDS             complement(28011..28637)
                     /locus_tag="OO328_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5930572.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            28952..30100
                     /locus_tag="OO328_001996"
     CDS             28952..30100
                     /locus_tag="OO328_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5930573.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7454 a         7456 c         8138 g         7209 t
ORIGIN      
        1 gcgcttgcgt gatgcccgtt ttatgcgccg tattcgtaac ggcacggtgc caaatgtccc
       61 caatgtggaa gtcaccgcgc gccagctcga tttcctcgcc cgtgagttgt cctcacatcc
      121 gcaaaatctc tctgatggac agattcgtca gggactttcc gcaatggtgc agttgcttga
      181 gcattatttc tctgagcagg gggctggaca ggcgcgatat cgtttaatgc ggcggcgggc
      241 cagcaatgag caacgaagct ggcgctatct ggacatcatc aaccggatga ttgaccgacc
      301 tggagggcgc tcgtatcggg ttattttgct cggcctgttt gctactttat tgcaggcaaa
      361 aggcacattg cgactggata aagacgcgcg tccgttattg ctaattgaag atccagaaac
      421 ccgcctgcat ccgattatgc tttccgttgc ctggcatctg ttgaatcttt tgcctttaca
      481 gcgtattgct accaccaact ctggcgagct gctttcgtta actccagtgg aacatgtttg
      541 ccgcctggtg cgtgaatctt ctcgtgttgc cgcctggcgt ctggggccaa gtggcttgag
      601 caccgaagac agccgccgca tctcttttca cattcgtttt aatcgtccgt catcgctgtt
      661 tgctcgctgc tggttgctgg tggaagggga aacggaaacc tgggttatca atgaactggc
      721 gcgtcagtgc ggacatcatt ttgatgccga agggatcaag gtcattgagt ttgcccagtc
      781 cgggctaaag ccactggtta aatttgcccg ccgaatgggg attgaatggc atgtactggt
      841 cgatggcgat gaagcaggga agaaatatgc cgctacggta cgcagcctgt tgaataacga
      901 tcgggaagcc gaacgagaac atttaacggc gttaccggcg ctggatatgg aacattttat
      961 gtatcgccag ggattttccg atgtgttcca ccgcgtggcg caaatcccgg aaaatgtacc
     1021 gatgaatcta cgcaaaatta tctcgaaagc gatccatcgc tcttccaaac ccgatcttgc
     1081 cattgaagtg gcaatggagg caggacgtcg tggtgtggac tccgtaccaa cgctgctgaa
     1141 aaaaatgttc tcacgcgtgc tgtggctggc gcgcggtcgc gcggattaac cgcgaaacat
     1201 cgtggccatt tgtggctgaa tagcgtcgag catctcatag cgccgacggt attcagcccg
     1261 ttttttactg gcgatttcgg caatctcttt tcgtgctatc tgtgctggaa ggcggtaatg
     1321 gcgttcagca tcacatacgc cgccaaccga ctcccagaac gcgttgtaat cagcatggat
     1381 cttgccttct ttatcgcgat aacgcaggct gcggtaaata tgcgtttcat tgctgacggc
     1441 aataatctgc tctacctgca aacgttgggc aaacagacag gccgcttcca tcacgaggcg
     1501 tttgggaaat agcccgtggc aggctttcgt cgcattctgg atttcctgat gtggaatttc
     1561 ccattttgcg ccttgcagtc cgccaataaa catcgttctt ttcccctgat attcacacag
     1621 ggtaaacgtg atctctgcca gaggaatacc ttcgctgttg cggaacagga ttgtgctgtc
     1681 accttcttta tccattgaga tcatcatggt cagctcaagc gtgaactgct caccgttttt
     1741 gccttccagt ttagccagtt gcagcccggg ggtattcaaa tataagctga attcttccgc
     1801 cgacatacac ccgcggagta acgcataatg gtaacgtaac gcctccagca attgcttacg
     1861 gctaagattc gcggcaaggt aagggcgatg cagacgcaca ggcagtcgcg gctggcgcgt
     1921 taacaacaca ttgagattag gccagtggga aagttcgttc atccactcaa cgcttaaacg
     1981 cggcataatc aacgagcgca gcaaaaattt ctggcgaaaa ctacagcgat gccagaattt
     2041 acccggccga cactgtccac gtgccagact aagaaaaagt gacaggctgc tgagagattc
     2101 agatggcgta aaggtccgtt cagttagctg cgacatattc atgaaatcaa tggttataca
     2161 tgacgtcgat ttcaccattg cgtatcttaa ccaaacatca atagtgtgat tactaacgta
     2221 aattttaggg ttttgttgat atttcgttga agttaatgac ccggattggc atatggagta
     2281 ttcagaatat ttatgaaaaa gcggaaaacc gtgaagaagc gttacgttat tgcgctggtg
     2341 atagtcatcg ccggactgat tacgttatgg agaattctta acgcacccgt gccgacttat
     2401 cagacactga ttgtgcgccc cggtgattta cagcaaagcg tgctggcgac cggaaagctg
     2461 gacgcgctgc gtaaggttga cgtgggcgcg caggtcagcg gtcagttgaa aacgctgtcg
     2521 gtggcgattg gcgataaagt aaaaaaagac cagcttttag gggtcattga tcctgaacag
     2581 gctgaaaacc agatcaagga ggtcgaagca acgctgatgg agctacgcgc gcagcggcag
     2641 caggcggaag cggagctgaa actggcgcgg gtgacgtatt cccgtcagca acgtctggca
     2701 caaacgcaag cggtttcact gcaggatctc gataccgccg cgacggagat ggctgtgaaa
     2761 caggcgcaaa ttggcaccat tgacgcgcaa atcaagcgca atcaggcctc tctcgatacg
     2821 gctaaaacca atctcgatta cacccgtatc gttgccccga tggctgggga agtgacgcaa
     2881 atcaccactc tgcaaggcca gacggtgatt gccgcacagc aagcaccgaa cattctgacg
     2941 ctggcagata tgagcactat gctggtaaaa gcgcaggttt ctgaagcgga tgtaatccac
     3001 ctgaagccgg ggcaaaaagc ctggtttacg gtgcttggcg atccactgac gcgctacgag
     3061 gggcaaatca aggatgtgct accgacgccg gaaaaggtaa acgacgctat tttctattac
     3121 gcccgttttg aagtacccaa ccccaatggt ttgctgcggc tggatatgac cgcgcaagtg
     3181 catattcagc tcaccgatgt gaaaaatgtg ttgacgatcc ctctgtcggc gttaggcgat
     3241 ccggttggcg ataatcgtta taaagtcaaa ttgttgcgta atggtgaaac acgcgagcgt
     3301 gaagtgacga ttggcgcacg taacgatacc gatgttgaga ttgtcaaagg gctggaagcg
     3361 ggcgatgaag tggtgattgg tgaggccaaa ccaggagctg cacaatgacg cctttgctcg
     3421 aattaaagga tattcgtcgc agctatcctg ccggtgatga gcaggttgag gtgctgaagg
     3481 gcatcagcct caatatttat gcgggtgaga tggtcgcgat tgttggcgct tcgggttccg
     3541 gtaaatcgac cctgatgaat attctcggct gcctggataa ggccaccagc ggcacctatc
     3601 gcgtagccgg tcaggatatt gccacgctgg acgccgatgc gctggcgcaa ctgcgccgcg
     3661 agcatttcgg ctttattttc cagcgttacc atttgcttcc gcatttaacc gtcgagcaga
     3721 acgttgaagt acccgccgtc tatgccggtc ttgagcggaa acagcgactg cttcgtgccc
     3781 aggagttgct gcaacggctg gggctggaag accgtacaga gtattatccg gcacagcttt
     3841 cgggtggtca gcaacagcgc gtcagcatcg cgcgggcatt gatgaacggc ggtcaggtaa
     3901 ttcttgccga tgaaccgacc ggcgcactgg acagccattc tggcgaagag gtgatggcga
     3961 tcctgcatca gctgcgcgat cgcgggcata cggtgattat cgtcacccac gatccgcagg
     4021 tcgctgctca ggccgagcgg gtgatcgaaa ttcgcgacgg cgaaattgtg cgcaatcctc
     4081 ccgccattga aaaagtgaat gttgctggcg ggacggagcc tgttgtcaat acagtgtctg
     4141 gctggcggca gtttgtcagc ggttttaacg aggcgctgac gatggcatgg cgggcgctgg
     4201 cagcgaataa aatgcgtact ttactgacca tgctggggat tattatcggt attgcgtcgg
     4261 tggtttccat tgtcgtggtg ggtgacgccg ccaaacaaat ggtgctggcg gatattcgtt
     4321 ctattggtac gaatactatt gatgtctatc ccgggaaaga ttttggcgat gacgatccgc
     4381 aatatcagca ggcgctgaag tacgacgact taatcgccat ccaaaaacaa ccgtgggtcg
     4441 cctcagccac acctgccgtc tcgcaaaacc tgcgcctgcg ttataacaat gttgatgttg
     4501 ctgccagtgc caatggcgtg agcggcgatt attttaatgt ctatggcatg accttcagtg
     4561 aaggaaacac ctttaatcag gagcagctga acggtcgtgc gcaggtcgtg gttctcgaca
     4621 gtaatactcg ccgccagctt ttcccccata aagcagatgt ggttggcgag gtgattctgg
     4681 tcggcaatat gcccgccaga gtcattggtg tggcggaaga aaaacagtcg atgtttggta
     4741 gcagtaaagt gctgcgtgtc tggctacctt acagcacgat gtccgggcga gttatgggcc
     4801 agtcgtggct taactccatt actgtcaggg tgaaagaagg atttgacagc gccgaggcgg
     4861 aacagcaact cacgcgttta ctttcactgc gccacggtaa gaaggatttc tttacctgga
     4921 acatggacgg cgtcttgaaa actgttgaaa agaccacacg tactttacaa ctgtttctga
     4981 cgctggtggc ggtgatttcg ctggtggtgg gcggtattgg tgtaatgaat attatgctgg
     5041 tgtcagtgac cgagcggacg cgggaaattg gcattcgcat ggctgtaggt gcgcgagcaa
     5101 gcgatgtttt gcaacagttc ctgatcgaag ccgtactggt ttgcctggtc ggtggcgcgt
     5161 tgggaataac actgtcactg ttaattgctt tcaccttgca gcttttctta cccggctggg
     5221 agattggttt ttcaccgttg gcgctgctgc tggcgtttct ctgctcgacg gccaccggga
     5281 ttttatttgg ctggttaccc gcacgaaatg cggcacgact ggatccagta gatgctctgg
     5341 cacgggagta atttttgaga taaaaatgcc agccgatcgg gctggcattt tgcctttagg
     5401 atgtacacaa tgagacagaa gagctatgcg actgccgctt ctacttcgac gggcacaata
     5461 acactggcgt gattgccttt tggcccctgg tggacatcaa actgaacgga ttgtccagct
     5521 tttagcgttc tgtaaccatc catctgaatg gtggaataat gagcgaaaat atcttcgccg
     5581 ccgccttcag ggcagatgaa accaaaccct ttggcattgt tgaaccactt aacagtaccc
     5641 ttttccatgc ttcgacatcc ttcgcaaatc ttatacaagt aagatggaat aaaccggggt
     5701 cagagagggg gctgttcaaa acctcgccaa ctctagaaat acaatttaga gaattagggc
     5761 gagccgtcaa gcatttgaca ggggccaggg ggcaggtatg aatcaaaaat ttgaagcagt
     5821 taacgctatt gacaggaatg tgacagatgt cgctgatgcc aacgatagat gatagttatc
     5881 tatcatgtgg agtagattgg tcaggcaaat aagctcttgt cagcggcagg gcgttctgcc
     5941 gataaccgta accgaagatg ataactgaca atgggtaaaa cgaacgactg gctggacttt
     6001 gatcaactgg cggaagaaaa agttcgcgac gcgctaaaac cgccatctat gtataaagtg
     6061 atattagtca atgatgatta cactccgatg gagtttgtta ttgacgtgtt acaaaaattc
     6121 ttttcttatg atgtagaacg tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag
     6181 gccatttgcg gagtctttac cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag
     6241 tacgcgaggg agaatgagca tccattgctg tgtacgctag aaaaagcctg aatgcaggca
     6301 taaaaattgg gggaggtgcc tatgctcaat caagaactgg aactcagttt aaatatggct
     6361 ttcgccagag cgcgcgagca ccgtcatgag tttatgaccg tcgagcactt gttactggcg
     6421 ctgctcagta acccatctgc ccgggaggcg ctggaagcgt gttctgtgga tttggttgcg
     6481 ctccgtcagg aactggaagc ctttattgaa caaaccacac ccgttctgcc tgccagtgaa
     6541 gaggagcgcg acacacagcc gacgctgagt tttcagcgtg tactgcaacg tgcggtcttc
     6601 catgtccagt cctccggtcg caatgaggtt accggtgcaa acgttctggt cgctatcttc
     6661 agcgaacagg agtcgcaggc ggcatatctg ttgcgtaaac atgaagtcag ccgtctcgat
     6721 gtggtgaact ttatctctca tggcacgcgt aaagacgagc cgacacagtc ttctgatcct
     6781 ggcagccagc caaacagcga agaacaagct ggtggggagg aacgtatgga gaatttcacg
     6841 acgaacctga accagcttgc gcgtgtgggc ggaatcgacc cactaattgg tcgtgagaaa
     6901 gagctggagc gtgctattca ggttctctgc cgtcgccgta aaaacaaccc gctgctggtg
     6961 ggggaatctg gtgtcggtaa aaccgcgatt gcagaaggtc ttgcctggcg aattgttcag
     7021 ggtgatgtgc cggaagtgat ggctgactgt acgatttact ctctcgatat cggttctctg
     7081 ttagcgggca caaaatatcg cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag
     7141 ctggagcagg acactaacag catcctgttt attgatgaga tccacaccat tatcggtgcg
     7201 ggtgcagcgt ctggtggtca ggtcgatgcg gctaacctaa tcaaaccgtt gctctccagc
     7261 ggtaaaattc gcgtaattgg ttcgacaacc tatcaggagt tcagcaacat tttcgagaaa
     7321 gaccgtgctc tggcgcgtcg cttccagaaa attgatatta ctgaaccgtc gatcgaagaa
     7381 actgttcaaa tcatcaatgg cctgaaaccg aagtatgaag cgcaccacga cgtgcgttat
     7441 accgcaaaag cggtgcgtgc ggcggtagag ctggcggtga aatacattaa cgatcgtcat
     7501 ctgccggata aagccattga tgttatcgac gaagcaggcg ctcgcgcacg tctgatgccg
     7561 gtaagcaaac gcaagaaaac cgttaatgtg gcggatattg agtccgtggt ggcccgtatt
     7621 gcacgcattc cagagaagag tgtttctcag agtgaccgcg ataccctgaa aaaactcagc
     7681 gatcgcctga aaatgctggt cttcggccag gataaagcca ttgaggcgct gactgaagcc
     7741 attaagatgg cgcgtgcagg tttaggtcac gaacataaac cggtcggttc gttcctgttt
     7801 gccggtccta ccggggtcgg gaaaacagag gtgacggtac agctttcgaa agcgttgggc
     7861 attgagcttc tgcgctttga tatgtccgag tatatggaac gccataccgt cagccgtctg
     7921 attggtgcgc ctccgggata cgttggtttt gatcagggcg gtttgctgac cgatgcggta
     7981 atcaagcatc cacatgcggt gctgctgctg gacgaaatcg agaaagcgca tccggacgtg
     8041 ttcaatattc tgttgcaggt gatggacaac ggtacgctga ccgataacaa cggacgcaaa
     8101 gcggacttcc gtaacgtggt gctggtgatg accactaacg ccggggtacg ggaaactgag
     8161 cgcaaatcca ttggtcttat ccaccaggat aacagcaccg atgcgatgga agagatcaag
     8221 aagatcttta caccggaatt ccgtaaccgt ctcgacaaca ttatctggtt cgatcatctg
     8281 tcaactgacg tgatccatca ggtggtggat aaattcatcg tcgagttgca ggttcagctg
     8341 gatcagaaag gtgtttctct ggaagtgagc caggaagcgc gtaactggct ggccgagaaa
     8401 ggttacgacc gggcaatggg cgcacgtccg atggcgcgtg tcatccagga caacctgaaa
     8461 aaaccgctcg ccaacgaact gctgtttggt tcgctggtgg acggcggtca ggtgacggtt
     8521 gcgctggata aagagaaaaa tgagctgact tacggattcc agagtgcaca aaagcacaag
     8581 gcggaagcag cgcattaatc tgattgtcag gtaggttggt gaagtccgta atctcgtcag
     8641 gggttacgga ctttttattt atggggtgga acggtgaaaa ccctattttt ggaggtgaag
     8701 gtaagttgtt gataattagt gctgctggaa ggtaaggata aaaaagggtg ctgcaggaga
     8761 atgggatggt tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg
     8821 tacaggcagc ttagaaatcg acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg
     8881 tacaggcagc ttagaaattc acaggtaaca tactccaccc gcccaccatg ttcactgccg
     8941 tacagacaga taaaatgcga aaaaaaagct cgcactttcg tacgagctct tctttaaata
     9001 tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt
     9061 gctccttcag ccactcggac acctcaccaa attgttttgc taccaaacct catgggtggc
     9121 aacggggcgc tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt
     9181 attgtttgct catactgtaa acaacttgtg cagtatatct acatcgagac aagttatgga
     9241 cttatacttc caaagtactt catacatatc acaaaataaa aaggccggtt aaaccgacct
     9301 tttactcgtt ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca
     9361 atgcggcctt tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg
     9421 atgcggatgt agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt
     9481 tctaactcta cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca
     9541 atattgtctt ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca
     9601 agataatgcc gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg
     9661 ttcggcacat tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg
     9721 agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt
     9781 aatgagcgta accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa
     9841 acattcattc ttgtggtgaa aacaagcacc gtggtaccca gaaattattc ggcaatcgtc
     9901 cgaggcgcat ttgattgaga taattaaggt aatcccggcg gggaatttcg caggcaccaa
     9961 gcgatgctgt gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa
    10021 attcctcaca gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg
    10081 actcgccaca aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt
    10141 cacgccagac ttcaatggag tgggcatgac cgagttcgtg aaggcgatgg taggcttcga
    10201 ccacgccacg cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct
    10261 gaccaaaagc gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac
    10321 tacggctgat atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga
    10381 ggatggggtc gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc
    10441 gcgcagggct aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt
    10501 ccggggaagg gaaggctatt gaatggcgag aaagctgaac caagcgcatg accgcaaaac
    10561 tccacgcaag tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg
    10621 cccctgtctg gcaagcagtt ctgcgtgagt accttgctca ataatttgcc cgttgtccat
    10681 cactattatt tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt
    10741 tttctcacgc atcatttttg caagcaattc aaggatctgg ctttcggttg tggcatctaa
    10801 gccttcggta ggttcatcca gcaacaccag tggcgcatca tgtaacagcg tacgggcgat
    10861 agccagacgg cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt
    10921 gagacctgca tcctcgagca gcttctccag accaacgcga cgcaagatct ccgacagagc
    10981 ctcatcacta ctgccaggcg aggcgagtaa cagattatca cgcagtgtgg cgctaaacag
    11041 atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc
    11101 tatggggcta tcgttaagca aaatctcgcc ctgttgcggg tcccatgcgc gggtcagcag
    11161 ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga atcgctatat gttccccggc
    11221 gtttacctga agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg
    11281 aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat cagggaaggt
    11341 gacctccggt ttttgatccg ttaagtcaga gatacgtacg gcagaggcaa tgacttgccc
    11401 cagatgctga aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcacaaaa
    11461 gacaaacagg gcaattaatg cgccgggttg agcattgccg ccaacgccgc cagacgccat
    11521 ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc attatcgctt gcgacaatgc
    11581 ggtcagttca gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg
    11641 cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca gcttgccctt gcagccaggc
    11701 cgtcagttgt tggcgatact gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc
    11761 cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt aacataacgc tgcccagcgt
    11821 aaaggcgagg gtgaaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa
    11881 agcgcccacc agcggcgaga taacgcgcag gtaaagatga tcgagcgtat caacatccgc
    11941 caccacgcgg ttgagcaatt cgccctgacg atagcgcgcc agtccggcag gggagagggg
    12001 caacaatttg ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg
    12061 acttaccaga cgttcaaaat aacgcccggc agtacgggtg attgcggcac cacgcacgcc
    12121 cgcagcgggt agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc
    12181 cgagaggaac cagccggaaa gtgtcaacag accgatactg gcgagcagcg tcacaattgc
    12241 cagcacaata ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag
    12301 caaagcgcgc atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc
    12361 agccacactt aattccgcgt aacgtccttg ctcaataatc cggccatcct gcataaccca
    12421 aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg
    12481 cagagaggcg gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc
    12541 agcgggttca tccaacagta atagcgaaca gggatttagt aacgcacggg ccaccgccac
    12601 gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg ccaacaggtg tatcaacgcc
    12661 ttgtggcagg agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc
    12721 ttgttcgctg gcatcaggtc gcgccagtag tacgttatcc cgcaattttg ctgccggtaa
    12781 ttgtgggttt tgcccaaccc aggagagatg tttacgccat gattctggtg ataaatcacg
    12841 taattctatc ccgttgattc gtaatgatcc ctgatatgag agaaaaccag aaagcgcgtt
    12901 cagcagtgag cttttacctg aaccgctgcg gccaaccaac actgcacgtt ggcctgctgg
    12961 caaagtaaag ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc
    13021 ctcggcctca atggtcagcg gatcggtcaa tgctaattcc gcctcaccgc gctgcggatg
    13081 ggcgagcggg gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt
    13141 agcatgataa aacgtaccga gatcgcgtaa tggctggaaa aactctggcg caaggatcag
    13201 ggccagaaaa cctgcagcca gcgtcacccc agtatcgtag tgaccaaaat ccagctcgcc
    13261 gagataggaa aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc
    13321 gagaatgccg gaggataaaa acgccagtcg tagcacttcc attgtccgtt ggcggaaatc
    13381 ttccgaagca gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt
    13441 ttccatgccg cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt
    13501 acgccggtta gcatcggcag cccccattcc aaccagcgcc ataaacaacg gaattagcgg
    13561 tgctgtgccc agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat
    13621 cagcaacggc accgacactg ccagcgccat ttgcggcaga tagcgtgcat agtaatcatg
    13681 catatcgtca atttgctcga gtaccagcgt cgcccagctc cccgcaggtt taccctgaat
    13741 ccacgctggc cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat
    13801 atgctgcccg gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcaatac
    13861 aaaggtcaga accagtaacg taaagggaag cagcagggct tcacggggaa tattctccat
    13921 aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg gcaatgatca atatgccgct
    13981 tacaaagccc agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt
    14041 taaccagcgg gttaactctt tttgacgaga tttattcatt gcacgcttag caggtgagtt
    14101 atcagaatta tttgcagagc aatgttacaa cggggaaaaa ataaaggcga cccatagtcg
    14161 caaggtgtcg ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa
    14221 ccatcgaggt agcgttccgc atcaagtgct gccatgcagc cagtaccggc cgaagtaatg
    14281 gcctggcgat aaatgtgatc catcacgtcg cctgcggcaa agacgccagg aatgctggtc
    14341 tgggtggcat taccatgaat acccgactgt actttgatgt agccgttttc cagttccagc
    14401 tgcccttcga aaatcgcagt attcgggctg tgaccgatag caacaaacag accggcaacg
    14461 tcgagtgact cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacaccc
    14521 atttgatcgc cggtcacttc ttccagcgta cggttggtgt gcagaatgat gttgccgttc
    14581 tccactttat ccatcagacg cttaataagg attttttccg cgcggaaacc gtcacggcgg
    14641 tgaatcagat gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta
    14701 ttgccgccgc cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggttgca
    14761 caagcagaaa ccccacggcc tttaaaggct tcttcagagg gcaggccgag atagcgtgca
    14821 gaagctccgg tggcaataat cagcgcgtcg caagtgtatt cgccgttatc gccattcaga
    14881 cggaacggac ggttttgcag atccaccttg ttgatatgat caaaaatgat ctcagtttca
    14941 aacttggtgg catgttcgtg catgcgcgcc attaataacg gaccggtcag atcgtttgga
    15001 tcaccaggcc agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg
    15061 gtaatcagca caggttgcag gttggcgcgc gccgcgtaga cagcagcggt gtatcccgcc
    15121 gggcctgaac ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag
    15181 ttgttggcag acaatgggca ggattgtagg gaatttacag acgtaaaaaa agagtatgac
    15241 gattttgtta acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca
    15301 ttatcacctc tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc
    15361 ccctgttaat gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc
    15421 ctggtgtttt gcgaaaacat tcgaggaaga aaaaaacagt attcttatat gcgcataacc
    15481 atgtatgtaa ataccatgtt taccgtgcta gtgaaatcta cgtatggcgt ggacagacgc
    15541 cattcgtgat gtcgatagct gccacaaggc aacggtcttc tcaccgtaga cccaggcatt
    15601 gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg gtagcgactc tgaacagtga
    15661 tgtttcaggg tcagacagga gtagggaagg aatacagaga gacaataata atggtagata
    15721 gcaagaagcg ccctggcaaa gatctcgacc gtatcgatcg taacattctt aatgagttgc
    15781 aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg tgtgggactt tccccaacgc
    15841 cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt tattcagggc tatacggcgc
    15901 tgcttaaccc ccattatctg gatgcatcac ttctggtatt cgttgagatt actctgaatc
    15961 gtggcgcacc ggatgtgttt gaacaattca ataccgctgt acaaaaactt gaagaaattc
    16021 aggagtgtca tttagtatct ggtgatttcg actacctgtt gaaaacacgc gtgccggata
    16081 tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg tctgcctggc gtcaatgaca
    16141 cacggacata cgttgttatg gaagaagtca agcagagtaa tcgtctggtt attaagacgc
    16201 gctaacacgg aacaggtgca aaatcggcgt attttgatta cactcctgtt aatccataca
    16261 gcaacagtac tggggtaacc tggtactgtt gtccgtttta gcatcgggca ggaaaagcct
    16321 gtaacctgga gagcctttct tgagccagga atacactgaa gacaaagaag tcacattgac
    16381 aaagttaagt agcggccgcc gccttctgga ggcattgctg atccttattg tcctgtttgc
    16441 cgtctggttg atggctgcct tactaagctt taacccttcg gaccccagct ggtcgcaaac
    16501 ggcctggcat gaacctatcc ataatttagg tgggatgccc ggtgcgtggt tggcagatac
    16561 gctgttcttt atttttggcg tgatggctta caccattccc gtcattattg tcggcggttg
    16621 ttggtttgcc tggcgtcatc agtccagcga cgaatacatt gattattttg ccgtttcgct
    16681 acgcatcatt ggcgttttgg cgctcatcct tacctcctgt ggtctggcgg caatcaacgc
    16741 tgacgatatc tggtattttg cctccggtgg cgtcattggc agcttactaa gcactacgct
    16801 acaaccactg ctacacagta gcgggggaac tattgcgctg ctctgcgttt gggcagcggg
    16861 cctgacgttg ttcaccggtt ggtcatgggt gaccattgct gaaaaactcg gcggctggat
    16921 tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc gatgatacct gggtcgatga
    16981 agatgagtat gaagacgacg aagagtatga agatgaaaat cacggcaaac agcatgaatc
    17041 acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt aaacggttgg cggaaaaatt
    17101 cattaatccg atggggcggc aaacagacgc tgcgttgttc tccggtaagc ggatggatga
    17161 tgacgaagag attacctaca ctgcacgcgg tgtggctgcc gacccggacg acgtcctatt
    17221 ttcgggcaat cgtgcaacgc agccagaata tgacgaatac gatccattat taaacagtgc
    17281 gccaattacc gaacctgtcg ctgtagcagc tgctgctacc acggcgacac aaagctgggc
    17341 tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc tctgttgatg ttccaccttc
    17401 gcaacctaca gtagcctggc agcctgtacc gggtccacaa acgggagagc cggttattgc
    17461 tcctgcaccg gaaggttacc cacagcagtc acaatatgcg cagcctgcag tgcaatataa
    17521 tgagccgctg caacaaccag tacagccgca gcagccgtat tatgcacctg cagctgaaca
    17581 acctgcgcaa cagccgtatt atgcccctgc gccagaacaa ccggtggcag gtaacgcctg
    17641 gcaagccgaa gagcagcaat ccacttttgc tccacagtct acataccaga ctgagcaaac
    17701 ttatcagcag ccagccgctc aggagccgtt gtaccaacag ccgcaacccg ttgaacagca
    17761 gcctgttgtg gagcctgaac ccgttgtaga agagacaaaa cccgcgcgtc cgccgcttta
    17821 ctattttgaa gaagtggaag agaagcgagc ccgtgaacgt gaacaacttg cggcctggta
    17881 tcaaccgatt ccagaaccgg ttaaagaacc agaaccgatc aaatcttcgc tgaaagcacc
    17941 ttctgttgca gcagtacctc cagtagaagc cgctgccgct gtttctccgc tggcatctgg
    18001 cgtgaaaaaa gcgacactgg cgacgggggc tgccgcaacc gttgccgcgc cagtcttcag
    18061 tctggcaaat agcggtggac cgcgtccgca ggtcaaagag gggattggcc cgcagttgcc
    18121 acgaccgaaa cgtatccgcg tgccaactcg tcgtgaattg gcgtcttacg gtattaagct
    18181 gccctcacag cgtgcggcgg aagaaaaagc ccgtgaagcc cagcgcaatc agtatgattc
    18241 tggcgatcag tataacgatg atgaaatcga tgcgatgcag caggatgaac tggcacgtca
    18301 gttcgcccag acacagcagc aacgctatgg cgaacagtat caacatgatg tgcccgtaaa
    18361 cgcagaagat gcagatgctg cggcagaggc tgaactggct cgtcagtttg cgcaaactca
    18421 acaacaacgt tattccggcg aacaaccggc tggggcgaat ccgttctcgc tggatgattt
    18481 tgaattttcg ccaatgaaag cgttgctgga tgatggtcca cacgaaccgc tgtttacgcc
    18541 aattgttgaa cctgtacagc agccgcaaca accggttgca ccacagcagc agtatcagca
    18601 gccacaacag ccggttgcgc cacagcagca gtatcagcag ccacaacagc aggttgcgcc
    18661 gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca cagcagcaat atcagcagcc
    18721 gcaacaacca gttgcgccgc agccgcagta tcaacagccg caacaaccgg ttgcaccgca
    18781 gccgcagtat cagcagccac aacagccagt tgcgccacag ccgcaggata ccctgcttca
    18841 tccgctgttg atgcgtaatg gcgacagccg tccgttgcac aaaccgacga cgccactgcc
    18901 ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata cctttgcgct
    18961 tgaacaaatg gcgcgtctgg tggaagcgcg tctggctgat ttccgtatta aagccgatgt
    19021 cgttaattac tctccggggc cagttatcac tcgctttgaa cttaacctgg ctcctggcgt
    19081 aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt cgacggtggc
    19141 ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt taccaaataa
    19201 aaaacgacaa accgtttatc tgcgcgaagt tttggataac gccaaattcc gcgataatcc
    19261 gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg ttgccgatct
    19321 ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaaat ctgtcggtgt
    19381 gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc gtttcatcat
    19441 gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcacc tgttaacgga
    19501 agtcgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta acgagatgga
    19561 gcgtcggtat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt ataacgaaaa
    19621 aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga agccgggtga
    19681 cagtatggat gcccagcatc cggtgctgaa aaaagaacca tacattgtcg tgttggttga
    19741 cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga tagctcgtct
    19801 ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaactcagc gtccatcggt
    19861 tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct ttaccgtatc
    19921 cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac tgctgggtat
    19981 gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc atggtgcttt
    20041 tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg gtcgcccaca
    20101 gtatgttgat ggcatcacct ccgacagcga aagcgaaggt ggtgcgggtg gtttcgatgg
    20161 cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg aaaaacgcaa
    20221 agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg cagcgcgtat
    20281 tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg gtaatcgtga
    20341 agtgctggcc ccaccgccgt ttgactaatt aatgcattgc cggataaggc gcggtagcgt
    20401 cgcatccggc actctatcaa ctgaaaattc agtattttct tctttcctca agctgattat
    20461 tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt tgaggaataa
    20521 tgatgaaaaa aattgctatc acctgtgcat tactctcaag cttagtagca agcagcgttt
    20581 gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc ttccacgcca
    20641 gcttcacaca aaaagtgact gacggtagcg gcgcggcggt gcaggaaggt cagggcgatc
    20701 tgtgggtgaa acgtccaaac ttattcaact ggcatatgac acaacctgat gaaagcattc
    20761 tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa gctacggcaa
    20821 cctggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc aaccagtcca
    20881 gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg acgccgaaag
    20941 ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc acaatccatc
    21001 agtttagcgc ggtggagcag gacgatcagc gcagcagtta tcagctgaaa tcccagcaaa
    21061 atggggctgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc acggtagatg
    21121 atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc ggataatact
    21181 tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat cggtcagcaa
    21241 catttgctgg ctgcggggaa gccgttgccg cgcgctatcg aagccgggca tttgcattct
    21301 atgatcctct gggggccacc gggtaccggc aaaacaaccc tcgctgaagt gattgcccgc
    21361 tatgcgaacg ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa agagattcgc
    21421 gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat tctttttgtt
    21481 gacgaagttc accgtttcaa caaaagccag caggatgcat ttctgccaca tattgaagac
    21541 ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgagct taattcggca
    21601 ctgctttccc gtgcccgtgt ttatctgttg aaatccctga gtacagagga tattgagcaa
    21661 gtactaactc aggcgatgga agacaaaacc cgtggctatg gtggtcagga tattgttctg
    21721 ccagatgata cacgacgtgc cattgcagaa ctggtgaatg gcgacgcgcg ccgggcgtta
    21781 aatacgctgg aaatgatggc ggatatggcc gaagtctatg atagcggtaa gcgggtcctg
    21841 aagcctgaat tactgaccga aatcgccggt gaacgtagcg cccgctttga taacaaaggc
    21901 gatcgctttt acgatctaat ttccgcactg cataagtcgg tacgtggtag cgcacccgat
    21961 gcggcgctgt actggtatgc gcgaattatt accgctggtg gcgatccgtt atatgtcgcg
    22021 cgtcgctgtc tggcgattgc gtctgaagac gtcggtaatg ccgatccacg ggcgatgcag
    22081 gtggcaattg cggcctggga ttgctttact cgcgttggcc cggcggaagg tgaacgcgcc
    22141 attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt ctacactgcg
    22201 tttaaagccg cgctggccga tgctcgcgaa cgcccggatt atgacgtgcc ggttcatttg
    22261 cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg ggcaggaata tcgttatgct
    22321 catgatgaag caaacgctta tgctgccggt gaggtttact tcccgccgga aatagcacaa
    22381 acacgctatt atttcccgac aaacaggggc cttgaaggca agattggtga aaagctcgcc
    22441 tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg ttatcgttgc
    22501 ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc catttaattc
    22561 gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc agacgcagtc
    22621 gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg cgctcttgaa
    22681 gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg taactcccga
    22741 tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt acgtctggaa
    22801 gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc tttacaggct
    22861 gaaattcgcg atatcgcgct gaccatccct aacctgcctg cagatgaagt gccggtaggt
    22921 aaagacgaaa atgacaacgt tgaagtcagc cgctggggta ccccgcgtga gtttgacttt
    22981 gaagttcgtg accacgtgac gctgggtgaa atgcactctg gcctcgactt tgcagctgca
    23041 gttaagctga ctggttcccg ctttgtggta atgaaagggc agattgctcg catgcaccgc
    23101 gcactgtcgc agtttatgct ggatctgcat accgaacagc atggctacag tgagaactat
    23161 gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcagctgcc gaaatttgct
    23221 ggcgatctgt tccatactcg tccgctggaa gaagaagcag acaccagtaa ctatgcgctg
    23281 atcccaacgg cagaagttcc gctgactaac ctggtgcgcg gtgaaatcat cgatgaagat
    23341 gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc cggttcatat
    23401 ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga aatggtgcag
    23461 atcgtgcgcc cagaagactc aatggcggcg ctggaagaga tgactggtca tgcagaaaaa
    23521 gtcctgcagt tgctgggcct gccgtaccgt aaaatcatcc tttgcactgg cgacatgggc
    23581 tttggcgctt gcaaaactta cgacctggaa gtatggatcc cggcacagaa cacctaccgt
    23641 gagatctctt cctgctccaa cgtttgggat ttccaggcac gtcgtatgca ggcacgttgc
    23701 cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc tggtctggct
    23761 gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg tcgtattgaa
    23821 gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta atacccaatt
    23881 tttctgaatc taaaaagcgc ctttagggcg ctttttttgt ctccctttga taccgaacaa
    23941 taattactcc tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga
    24001 ttttcaaatt attctatgta tacaagccta tatagcgagc tgctatagaa ataattacac
    24061 aatacggttt gttactggaa tcaatcgtga tcaagcttga gtgagccatt atgaaaacga
    24121 aaatccctga tgcggtattg gctgctgagg agagtcgccg tggtttggta aaaacgacag
    24181 cgatcggcgg cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc
    24241 acgctgtcga tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg agcgcctgta
    24301 cagttaactg tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat
    24361 atgtcgaaac gacaataccg gcgatgacaa ttacgacggc ctacactagg ttcgcgcctg
    24421 cctgcgtggg cgttccatgc gtcaccgtgt ctacaatccg gaccgcctga aatatccgat
    24481 gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg aagaagccta
    24541 cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg agtctatcta
    24601 tctgaactat ggcaccggta cgctgggcgg caccatgacc cgctcctggc cgccgggaaa
    24661 taccctggtc gcgcgcctga tgaactgctg cggcggttat cttaaccatt acggtgacta
    24721 ctcttccgcg caaattgcgg aaggtttgaa ccatacctac ggcggctggg cagatggtaa
    24781 cagcccgtcg gatatcgaaa acagtaaatt ggtagtgctg tttggtaata acccaggcga
    24841 aacgcgaatg agtggcggtg gggtgactta ctatcttgaa caggcacgcc agaaatctaa
    24901 tgctcgcatg atcatcatcg atccgcgcta taccgacacc ggtgccgggc gcgaagatga
    24961 gtggatccct attcgtccag gaacagatgc cgcactggtt aacggtctgg cgtacgtcat
    25021 gatcactaaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg gctatgacga
    25081 gaaaaccctg cccgccagtg cgccgaaaaa tggtcattat aaagcttata ttctgggtga
    25141 agggccagat ggcgtggcta aaacaccgga atgggcctcg caaatcaccg gtgttccggc
    25201 agacaaaatc atcaaactgg ctcgtgaaat cggcagtacc aaaccggcgt ttatcagcca
    25261 gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta tctcgatgct
    25321 ggcgattctg accggtaacg ttggtattaa tggagggaac agcggcgcgc gtgaaggttc
    25381 atacagctta ccgtttgtcc gtatgccgac cttggaaaac ccgatccaga ccagcatttc
    25441 gatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacggcgc tgcgtgatgg
    25501 tgtgcgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact atgccggtaa
    25561 ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat gaaatcctcc aggatgataa
    25621 gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcggcga aatatgctga
    25681 catcctgctg cctgactgca ccgcttccga acagatggac tttgcactgg atgcatcctg
    25741 cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct ttgaatgtaa
    25801 gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc aacagtttac
    25861 tgaaggccgt acccaggaag agtggatgcg gcatctgtat gcccagtcgc gggaagcgat
    25921 tcctgaactg ccaacgtttg aagagttccg caagcagggg atctttaaaa agcgcgaccc
    25981 acaagggcat cacgttgctt ataaagcctt ccgtgaagat ccgcaggcaa acccactgac
    26041 tacgccatcg ggcaaaattg agatttattc gcaggcgctg gctgacattg ccgctacctg
    26101 ggaattgcca gaaggcgatg tgatcgatcc actgccgatc tacacgccgg gctttgaaag
    26161 ttatcaggat ccgctgaaca aacagtatcc gctgcagctt acaggtttcc actataaatc
    26221 tcgcgttcac tcaacttacg gcaacgttga tgtgctgaaa gcggcttgcc gtcaggaaat
    26281 gtggatcaac ccgcttgatg cccaaaaacg cggtatccac aacggcgata aagtcaggat
    26341 ctttaacgat cgtggtgagg ttcatattga ggcgaaagtg acgccacgaa tgatgccggg
    26401 cgtggtcgca ctgggtgaag gcgcctggta tgacccggat gcaaaacgtg tcgataaggg
    26461 cggttgtatc aacgtactga ccactcaacg tccgtctcct ctcgctaagg ggaatccgtc
    26521 acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca acccagtatg
    26581 gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg gcttgtaaag
    26641 actacaaaga tttgacgcca gaagtcagtt tccgccgcat ttatgaatat gctggcggcg
    26701 actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat ctgtcgattt
    26761 cgtgcaacca ctgcgaagat ccggcttgta ctaaagtctg cccgagcggt gcgatgcata
    26821 aacgcgaaga tggttttgtt gtggtcgatg aagatgtatg cattggctgc cgttactgtc
    26881 atatggcttg cccgtatggc gcaccgcaat ataacgaaac gaaaggccat atgaccaaat
    26941 gcgatggttg ttatgaccgt gttgcggagg gtaaaaagcc gatctgtgtt gaatcttgcc
    27001 cgctgcgggc gctggatttc ggccctatcg acgagctgcg taaaaaacat ggcgatctgg
    27061 cggccgttgc gccgttgccg cgagctcact ttaccaaacc gaatattgtg atcaaaccca
    27121 atgccaatag ccgcccgacc ggggatacca ctggctatct ggcaaacccg aaggaggtgt
    27181 gagatgggaa gtggatggca tgaatggccg ctgatgatct tcacggtctt cgggcaatgt
    27241 gtggcgggtg gttttatcgt tctggctttg gcgctgctca aaggcgacct gcgagcagaa
    27301 gcccagcagc gtgttatcgc ctgcatgttt ggcttatggg tactgatggg cattggcttt
    27361 atcgcctcta tgctccatct tggttcaccg atgcgcgcct ttaactcgct caaccgggta
    27421 ggggcttcag cactcagtaa cgaaatcgcc agcggttcga tcttttttgc tgtaggcggc
    27481 atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg cattgcgtac gctatggctg
    27541 gtggtgacga tggttcttgg cgtcgtcttt gtctggatga tggtgcgtgt gtataacagc
    27601 attgataccg ttccgacctg gtacagcatc tggacgccga tgggctgggc gatgcgtctg
    27661 ctgccagcca tttctgtact ggcactggtg gtgagtggcg tgatgtcagt gatgcagggc
    27721 acagagctgg caactattca tagttctgtg cagcaggccg cagcgctggt gccggactat
    27781 ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg ccctgtgctt gtggattgca
    27841 ccacagctaa aaggttatca gcctgcggtt ccgctactat ccgtctcatt cattctgctt
    27901 ctggcagggg aattaatcgg tcgcggcgta ttctatggtt tgcatatgac cgtggggatg
    27961 gccgtcgcaa gctaatcagt aacaaccggg gtttcggccc cggttctctt ttatttctgc
    28021 ttcgttaacg tgtcataact ggtcatcaaa ttacgataat caggaatatg gttagagaac
    28081 aacgtcgcca gtccttcaat atcattacgc cagtcacggt gcagctcgca agccacgccg
    28141 aaccatgtca tcaattgcgc accagcttgc gacatgcgat cccacgccga atgccgggta
    28201 atttcattaa aggtccctga tgcatcagtc acgacaaaga catcaaaccc ttcttcaatg
    28261 gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat aattaactgt
    28321 tttttacctg tcgctttgac tgcttttaca aaatcttcgt tatcccaggc gttaatattg
    28381 cccgggcgag caatgtaagg tgcatcgggg aattgtgctt tcagttctag aactaatggg
    28441 ccgttaggac cggtttcaaa actggtggta agaatagttg gtaagttgaa atacttagcc
    28501 aggtcaccca gcgccagcac gttattttta aacttatcgg gttcgatatc ccgtacaagg
    28561 gaaagtaaac cagcctggtg atcaacaagc aaaacggcag catcattttt atcaagacga
    28621 acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgtct gaaatcggtc
    28681 agacgcgatg cattgctctg aaagcataga cgggaaatat gagtttgctg tgaccatgaa
    28741 atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac aatcagatga
    28801 gattaatcat ctcttcagca ttagcgttat gaatgagtaa tataaatcga taataccgcc
    28861 gctgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc atgatgcgca
    28921 tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc ctgtcatgct
    28981 tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata cactcgtgcc
    29041 gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca gctcatccta
    29101 ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaaac gcattggctt
    29161 taaccgcagc tattatctgt cctccttcat ttttgctgct ggctgtgccg gccttggcct
    29221 gatgattggt ttctggagct ggttggcgtg gcgttttgtc gcgggcgtcg gctgtgccat
    29281 gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta accgtgggcg
    29341 tttgcttgct gcgtatatga tggtttatta cgtgggaacg tttttaggcc agttactggt
    29401 cagcaaagtc tcaaccgagc tgatgagcgt attgccgtgg gttacaggtt tgacattggc
    29461 agggatctta ccgctgttgt ttacgcgtgt cctgaatcag caggctgaaa accatgattc
    29521 gacgtcaatt acggcaatgc taaaactccg tcaggcgcgg cttggcgtga atggctgcat
    29581 tatctcagga atcgttctgg gatctctata tggcctgatg cctctgtacc tcaatcacaa
    29641 aggggtgagc aatgccagta ttggtttctg gatggcggta ctggtcagtg cgggtatcct
    29701 cggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg ttttgcgtgt
    29761 tcaggtcttt gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg cgatggcccc
    29821 agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga tggcctgggc
    29881 ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac caagccttac tgttgagcta
    29941 taccgtggga agtctgcttg gcccgtcatt tagcgctatg ctaatgcaga atttctccga
    30001 taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga tgctgcttcg
    30061 caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa gcaaagtgtg
    30121 aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt ttttccaaat
    30181 tttattcgtt taaaaataaa atgtgcagca ggttataatt ttgcatttcg ctatttccgc
    30241 acttcttatt tgccgcg
//
LOCUS       DAJTRN010000043        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRN010000043 DAJTRN010000000
VERSION     DAJTRN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO328_001997"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO328_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5930574.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO328_001998"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO328_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5930575.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO328_001999"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO328_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5930576.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO328_002000"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO328_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5930577.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO328_002001"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO328_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5930578.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO328_002002"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO328_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5930579.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO328_002003"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO328_002003"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5930580.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO328_002004"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO328_002004"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5930581.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO328_002005"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO328_002005"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5930582.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO328_002006"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO328_002006"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5930583.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO328_002007"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO328_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5930584.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO328_002008"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO328_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5930585.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO328_002009"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO328_002009"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5930586.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO328_002010"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO328_002010"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5930587.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO328_002011"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO328_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5930588.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO328_002012"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO328_002012"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5930589.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO328_002013"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO328_002013"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5930590.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO328_002014"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO328_002014"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5930591.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO328_002015"
     CDS             21622..22212
                     /locus_tag="OO328_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5930592.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO328_002016"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO328_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5930593.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO328_002017"
     CDS             22684..23379
                     /locus_tag="OO328_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5930594.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO328_002018"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO328_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5930595.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO328_002019"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO328_002019"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5930596.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO328_002020"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO328_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5930597.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO328_002021"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO328_002021"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5930598.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO328_002022"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO328_002022"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5930599.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7027 a         7824 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgccgcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTRN010000044        29523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRN010000044 DAJTRN010000000
VERSION     DAJTRN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(16..270)
                     /locus_tag="OO328_002023"
     CDS             complement(16..270)
                     /locus_tag="OO328_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5930600.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLTAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            580..2352
                     /gene="aspS"
                     /locus_tag="OO328_002024"
     CDS             580..2352
                     /gene="aspS"
                     /locus_tag="OO328_002024"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5930601.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2470..2922
                     /gene="nudB"
                     /locus_tag="OO328_002025"
     CDS             2470..2922
                     /gene="nudB"
                     /locus_tag="OO328_002025"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5930602.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2951..3691
                     /locus_tag="OO328_002026"
     CDS             2951..3691
                     /locus_tag="OO328_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5930603.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3726..4247
                     /gene="ruvC"
                     /locus_tag="OO328_002027"
     CDS             3726..4247
                     /gene="ruvC"
                     /locus_tag="OO328_002027"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5930604.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4249..4851)
                     /locus_tag="OO328_002028"
     CDS             complement(4249..4851)
                     /locus_tag="OO328_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5930605.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4921..4986
                     /gene="yobI"
                     /locus_tag="OO328_002029"
     CDS             4921..4986
                     /gene="yobI"
                     /locus_tag="OO328_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5930606.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5125..5736
                     /gene="ruvA"
                     /locus_tag="OO328_002030"
     CDS             5125..5736
                     /gene="ruvA"
                     /locus_tag="OO328_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5930607.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5745..6755
                     /gene="ruvB"
                     /locus_tag="OO328_002031"
     CDS             5745..6755
                     /gene="ruvB"
                     /locus_tag="OO328_002031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5930608.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6902..7687)
                     /gene="znuB"
                     /locus_tag="OO328_002032"
     CDS             complement(6902..7687)
                     /gene="znuB"
                     /locus_tag="OO328_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5930609.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7684..8439)
                     /gene="znuC"
                     /locus_tag="OO328_002033"
     CDS             complement(7684..8439)
                     /gene="znuC"
                     /locus_tag="OO328_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5930610.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8518..9450
                     /gene="znuA"
                     /locus_tag="OO328_002034"
     CDS             8518..9450
                     /gene="znuA"
                     /locus_tag="OO328_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5930611.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9466..10788
                     /gene="mepM"
                     /locus_tag="OO328_002035"
     CDS             9466..10788
                     /gene="mepM"
                     /locus_tag="OO328_002035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5930612.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10908..11879
                     /gene="lpxM"
                     /locus_tag="OO328_002036"
                     /gene_synonym="msbB"
     CDS             10908..11879
                     /gene="lpxM"
                     /locus_tag="OO328_002036"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5930613.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12010..13452)
                     /gene="pyk"
                     /locus_tag="OO328_002037"
     CDS             complement(12010..13452)
                     /gene="pyk"
                     /locus_tag="OO328_002037"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5930614.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13580..14449)
                     /gene="hexR"
                     /locus_tag="OO328_002038"
                     /gene_synonym="yebK"
     CDS             complement(13580..14449)
                     /gene="hexR"
                     /locus_tag="OO328_002038"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5930615.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14786..16261
                     /gene="zwf"
                     /locus_tag="OO328_002039"
     CDS             14786..16261
                     /gene="zwf"
                     /locus_tag="OO328_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5930616.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16496..18307
                     /gene="edd"
                     /locus_tag="OO328_002040"
     CDS             16496..18307
                     /gene="edd"
                     /locus_tag="OO328_002040"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5930617.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18344..18985
                     /gene="kdgA"
                     /locus_tag="OO328_002041"
                     /gene_synonym="eda"
     CDS             18344..18985
                     /gene="kdgA"
                     /locus_tag="OO328_002041"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5930618.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(19041..20219)
                     /gene="purT"
                     /locus_tag="OO328_002042"
     CDS             complement(19041..20219)
                     /gene="purT"
                     /locus_tag="OO328_002042"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5930619.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20353..20643
                     /gene="yebG"
                     /locus_tag="OO328_002043"
                     /pseudo
     CDS             20353..20643
                     /gene="yebG"
                     /locus_tag="OO328_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20710..21066
                     /locus_tag="OO328_002044"
     CDS             20710..21066
                     /locus_tag="OO328_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5930620.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21393..22052
                     /locus_tag="OO328_002045"
     CDS             21393..22052
                     /locus_tag="OO328_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5930621.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22262..24322
                     /gene="ptrB"
                     /locus_tag="OO328_002046"
     CDS             22262..24322
                     /gene="ptrB"
                     /locus_tag="OO328_002046"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5930622.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24319..24981)
                     /gene="exoX"
                     /locus_tag="OO328_002047"
     CDS             complement(24319..24981)
                     /gene="exoX"
                     /locus_tag="OO328_002047"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5930623.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(25005..25661)
                     /locus_tag="OO328_002048"
                     /pseudo
     CDS             complement(25005..25661)
                     /locus_tag="OO328_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25763..25993)
                     /gene="holE"
                     /locus_tag="OO328_002049"
     CDS             complement(25763..25993)
                     /gene="holE"
                     /locus_tag="OO328_002049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5930624.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26132..26506
                     /gene="yobA"
                     /locus_tag="OO328_002050"
     CDS             26132..26506
                     /gene="yobA"
                     /locus_tag="OO328_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5930625.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26510..27382
                     /gene="copD"
                     /locus_tag="OO328_002051"
                     /pseudo
     CDS             26510..27382
                     /gene="copD"
                     /locus_tag="OO328_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27395..27736
                     /locus_tag="OO328_002052"
     CDS             27395..27736
                     /locus_tag="OO328_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5930626.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28129..29206)
                     /locus_tag="OO328_002053"
                     /pseudo
     CDS             complement(28129..29206)
                     /locus_tag="OO328_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(29172..29453)
                     /locus_tag="OO328_002054"
     CDS             complement(29172..29453)
                     /locus_tag="OO328_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5930627.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7452 a         7247 c         7587 g         7237 t
ORIGIN      
        1 gccgggtctg gctggctaac accccattat aaggggtacg aataaagtaa cgggggcatc
       61 aaccggctgt tttaatgctt cggcgtaatc ggcagaccag ccaactcgca ccagaaacac
      121 gggctgaccg ctggcgcgaa atttcgccgt cagcttcccg gcgcgattaa ctacctcatc
      181 ggcagtatgt ggaccaccgg caaaaggtaa gatgccttct tgtaaatcga tcaccaccaa
      241 cgcagtggtt ttagcattaa gttcaagcat gctgactcct gaaaaaacaa aatcgtgcct
      301 cacaccttaa tgcgattagc cgatggcgga acgacaaatt ttgttaattt ttatgagttc
      361 gggcaacacc tttatagcaa actggcgtag agtctgattt caggcccgat ataaagtggt
      421 aacgaggcgt tccagcgcgg gccaggctct tacggtgcgg cagaatttcc agtataatag
      481 ccgccttttt tcattcagtt gtgacataca gctaacgctg cgacttggtc acctgcgcag
      541 cagggggcat ccgccggcag ctaagttaag ggatatctca tgcgtacaga atattgtgga
      601 cagctccgtt tgtcccacgt ggggcagcag gtgactctgt gtggttgggt caaccgtcgt
      661 cgtgatcttg gtagcctgat cttcatcgat atgcgcgacc gcgaaggtat cgtgcaggta
      721 tttttcgatc cggatcgtgc ggacgcgtta aagctggcct ctgaactgcg taatgagttc
      781 tgcattcagg ttacgggcac cgtacgtgcg cgtgacgaaa aaaatattaa ccgcgatatg
      841 gcgaccggcg aaattgaagt gctggcgtcc tcgctgacta tcatcaaccg cgcagatgtt
      901 ctgccgcttg actctaacca cgtcaacacc gaagaagcgc gtctgaaata ccgctacctc
      961 gacctgcgtc gcccggaaat ggcgcaacgc ctgaaaaccc gcgctaaaat caccagcctg
     1021 gtgcgccgtt ttatggatga ccacggcttc ctcgacatcg aaactccgat gctgaccaaa
     1081 gccacgccgg aaggcgcgcg tgactacctg gtgccttctc gtgtgcacaa aggtaaattc
     1141 tacgcactgc cgcaatcccc acagttgttc aaacagctgc tgatgatgtc cggttttgac
     1201 cgttactatc agatcgttaa atgcttccgt gacgaagacc tgcgcgctga tcgtcagcct
     1261 gaatttactc agatcgatgt ggaaacttct ttcatgaccg cgccgcaagt gcgtgaagtg
     1321 atggaagcgc tggtgcgtca tttgtggctg gaagtgaagg gcgtggatct gggcgatttc
     1381 ccggtaatga cctttgcgga agcagaacgc cgttatggtt ctgataaacc ggatctgcgt
     1441 aacccgatgg aactgaccga cgttgccgat ctgctgaaat ctgttgagtt tgcagtattt
     1501 gcggggccag cgaacgatcc gaaaggccgt gttgccgctc tacgtgttcc gggtggcgca
     1561 tcgctgaccc gtaagcagat cgacgaatac ggtaacttcg ttaaaatcta cggcgcgaaa
     1621 ggtctggctt acatcaaagt taacgaacgc gcgaaaggtc tggaaggtat caacagcccg
     1681 gtagcgaagt tccttaatgc agaaatcatc gaagccatcc tggatcgtac tgccgcgcaa
     1741 gatggcgata tgattttctt cggtgccgac aacaagaaaa ttgttgccga cgggatgggc
     1801 gcactgctcc tgaaagtggg taaagacctt ggtctgaccg acgaaagcaa atgggcaccg
     1861 ctgtgggtta tcgacttccc gatgtttgaa gacgacggtg aaggcggcct gacggcaatg
     1921 caccatccgt tcacctcacc gaaagatatg actgctgcag aactgaaagc tgcaccggaa
     1981 aatgcggtgg cgaacgctta cgatatggtc atcaatggtt acgaagtggg cggtggttca
     2041 gtacgtatcc ataatggtga tatgcagcag acggtgtttg gtattctggg tatcaacgaa
     2101 gaggaacagc gcgagaaatt cggcttcctg ctcgacgccc tgaaatacgg tactccgccg
     2161 cacgcaggtc tggcattcgg tcttgaccgt ctgaccatgc tgctgactgg caccgacaat
     2221 atccgtgacg ttatcgcctt cccgaaaacc acggcggcag cgtgtctgat gactgaagca
     2281 ccgagctttg ctaacccgac tgcactggct gagctgagca ttcaggttgt gaagaaggct
     2341 gagaataact gatatgactc aaatacacga aatcattcgc gttgcatcaa ggcggcaact
     2401 gagtgaactc ccatgagcat agataactat gtgaatggga tgagcgaagg cagtcaacga
     2461 agaggcagcg tgaaggataa agtgtataag cgtcccgttt cgatcttagt ggtcatctat
     2521 gcacaagata cgaaacgggt gctgatgttg cagcggcgtg acgatcccga tttctggcag
     2581 tcggtaaccg gcagcgtgga agagggtgaa accgcgccgc aagctgccat gcgcgaagta
     2641 aaggaagagg ttaccattga tgttgtcgct gaacaactga ccttaattga ctgtcagcgc
     2701 acggtagagt ttgaaatttt ttcacattta cgtcatcgct atgcgccggg cgtgacgcgt
     2761 aatacggaat catggttctg tcttgcgctt ccgcacgagc ggcagatcgt tttcactgaa
     2821 catctggctt acaagtggct tgatgcgcct gctgcggcgg cgctcactaa gtcctggagc
     2881 aaccggcagg cgattgaaca gtttgtaatt aacgctgcct gaacaggcag ggctatctgg
     2941 agatattttt atggcaggtc atagtaaatg ggccaacacc agacatcgta aagctgcgca
     3001 ggatgctaag cgcggtaaaa tcttcactaa aatcattcgt gagctggtaa ccgcggctaa
     3061 gctgggcggt ggcgatccgg acgctaaccc gcgtctgcgt gcggcggtgg ataaagcact
     3121 gtctaacaac atgacccgtg acacactgaa ccgcgcaatt gcacgtggtg tgggcggtga
     3181 tgatgatgca aacatggaaa ccatcatcta cgaaggttac ggtcctggcg gcacggcaat
     3241 catgattgaa tgtctgtctg acaaccgcaa ccgtaccgtt gctgaagtgc gtcacgcatt
     3301 cagcaaatgt ggcggtaacc tcggcactga cggttccgta gcttatctgt tcagcaaaaa
     3361 aggcgtgatc tccttcgaga aaggcgacga agacaccatc atggaagcag ctctggaagc
     3421 aggtgctgaa gacgttgtga catatgatga cggcgcgatt gatgtctaca ctgcatggga
     3481 agaaatgggt aaagtgcgcg acgctctgga agcggcaggt ctgaaagcag acagcgcgga
     3541 agtttccatg atcccgtcca ccaaagctga tatggatgca gaaaccgcgc cgaaactgat
     3601 gcgtctgatc gatatgctgg aagattgcga cgacgtgcag gaagtttacc ataacggtga
     3661 aatctctgat gaggttgcag cgactctctg atgatgcctg ctaaacagca aaacggagac
     3721 gcgtgatggc tattattctc ggcattgacc cgggttcgcg cgtgaccggc tacggcgtca
     3781 tccgccaggt aggtaggcaa ctgtcctacc tgggtagcgg atgcatacgc accaaagtgg
     3841 atgatttacc gtctcgtctg aagctcatct atgcgggcgt gacggaaatc atcacccagt
     3901 tccagcctga ttatttcgcc attgaacaag tctttatggc gaagaacgct gactcagccc
     3961 tgaaactggg ccaggcgcgc ggcgtggcga ttgttgcggc ggtgaatcag gaattgccgg
     4021 tatttgaata cgcggcacgt caggtaaagc aaacggtggt aggtatgggt agtgccgaaa
     4081 aaagccaggt gcagcatatg gtccgcacct tgctgaaact gcccgctaat ccacaggcgg
     4141 atgccgccga tgcgctggcg attgccatca cccactgcca cgttagtcag aatgcgatgc
     4201 agatgagcga atcgcggctg aacctgacga gagggcgact gcgttaagtt ataccgcctc
     4261 ggtcagttcc ggctgaggcg tttcaactcc ctccgcgtga atcaacacca aacccggatg
     4321 atgccacaaa ctggctggcg tgacggtttt acgctcccac ggaatagaac ctaagaacca
     4381 gaatttccag aaagtgagtt ttgcattcgg attgctattt aacagttgtc caaacgtctc
     4441 tatttcaccc accggaatac atagcgcctc tttataaata tcaaagacaa attttgaaca
     4501 gaactggcgc gaagattcgt atttaaaacc cgtgtggtaa agtttgcgta gccgggaggg
     4561 aacctgttca acaattcgtt gtttttgttg ttctgtcagc ccagcgtcta atcgctttat
     4621 agcatagcgt tgattagacg aacgtttaat aaaacgagat agcgtggtga tggttgaaag
     4681 gggaacccgg ctttctgcaa ccaggaagtc ttcaccgtta tgaccgataa tgatcccgac
     4741 gtgattactc cagcaatttg atgcagctga tatttgacca aataaggcag cacctataca
     4801 tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca cgttgtcatt
     4861 ccattgaaat agatacaaat atacgtaata gcataaaaaa attatttttc aggagtcgca
     4921 atgtatattt ttattacgca tttcttcact gaatatgtaa tattcaaata tttgcttcca
     4981 atataacctg ttgaataaat tatactgtgc catttttcag atcatcggga cacctcgcaa
     5041 gttttcttca tccttcgctg gatatctatc cagcattttt ttatcataca gcattatctt
     5101 tgattcatta cgcaggagcg tcatgtgata ggcagactca gaggcatcat cattgaaaaa
     5161 caacccccgc tggtgttaat tgaagtgggc ggcgtaggct atgaagtgca tatgccgatg
     5221 acctgttttt atgaactccc tgaagcgggt caggaagcga tcgttttcac ccactttgtg
     5281 gtgcgtgaag acgcgcaact gctgtacggt tttaacaata aacaagaacg cacgttgttc
     5341 aaagagttga tcaaaaccaa cggcgtcggc ccgaagttgg ccctggcgat cctctccgga
     5401 atgtcagcgc agcagttcgt taatgccgtt gagcgtgaag aagtgggggc actggtgaaa
     5461 ctgccgggta ttggcaaaaa aaccgccgaa cgcttgattg ttgaaatgaa agaccgattt
     5521 aaaggtttgc atggcgatct ctttacgcca gccgccgacc tggtactcac gtctcctgct
     5581 agcccggcga ccgacgatgc tgaacaagaa gcagttgccg cgctggtggc gctaggctat
     5641 aaaccacaag aagcaagccg catggtgagc aaaatcgctc gccctgacac cagcagtgaa
     5701 acattaattc gcgaagccct acgcgccgcg ttatgaggta aaggatgatt gaagcagacc
     5761 gtctgatttc tgccggtacc actttgccgg aagatgtagc agatcgcgcc attcgcccta
     5821 aattactgga agagtatgtt ggtcagccgc aggttcgttc acagatggag attttcatca
     5881 aagcagcgaa actgcgcggc gatgccctcg atcatttgtt gatttttggt cctccggggt
     5941 tgggtaaaac tacgcttgcc aacattgtcg ccaatgaaat gggcgttaat ttacgcacga
     6001 cttctggtcc ggtgctggaa aaggcgggcg atttggctgc gatgctcact aaccttgaac
     6061 cgcatgacgt gctgtttatt gatgagatcc accgtttatc gccagttgtt gaagaagtgc
     6121 tgtacccggc aatggaagac taccaactgg atatcatgat tggtgaaggt ccggcggcac
     6181 gctccattaa aattgatttg ccgccgttta ccctgattgg cgcaaccacg cgcgcaggtt
     6241 cgctgacatc accgttgcgc gaccgttttg gtattgtgca acgtctggag ttttatcagg
     6301 tgccggatct gcaatatatc gtcagtcgca gtgcacgctt tatggggctt gagatgagtg
     6361 atgacggtgc gcaggaagta gctcgtcgcg ctcgcggtac gccacgtatt gccaaccgtc
     6421 tgctgcgtcg agtgcgtgat ttcgccgaag tgaagcacga tggcaccatc tcggcagata
     6481 tcgctgctca ggcgctggat atgttgaatg tcgatgctga aggtttcgat tatatggacc
     6541 gcaaattgtt gctggcggta atcgataagt tctttggtgg gccggtaggt ctggataacc
     6601 tggcggcagc cattggcgaa gaacgtgaaa ccattgaaga tgtactggaa ccttatttga
     6661 ttcagcaagg ctttttgcag cgtacaccgc gcgggcgtat ggcgacgacg cgggcgtgga
     6721 atcactttgg cataacgccg ccagaaatgc cgtaagtcgg attggtcgct cgcgccgcat
     6781 ccgacactgg cagtgtgtct gatgcgacac tgttcgcgtc ttatcaggcc agccagagat
     6841 agcgaaccgt aggtcggata aggcgctcgc gccgcatccg acaaatgtgt tcagcgatag
     6901 attagctggc ctgctttttc atcatactga taataaataa cagtgccgca catagcacca
     6961 ccgacggacc cgccggcgta tcgtaaaacg cggaaaaggt taaaccgcca gttactgcca
     7021 ccatccccac caaaacagcg acaccagcca tctgttccgg cgtacgggca aagcgacgcg
     7081 cagtagctgc aggaataatc agcaacgaag taataatcaa cgcaccgaca aatttcatcg
     7141 ctacaccaat cgtcaatgcc gtcaccagca tcaacaacaa tttcacttgc tgtaatttca
     7201 caccatcaac aaacgccaga tccgggctaa tcgtcatcga cagcaaattg cgccattgcc
     7261 agaacaaaat agccaccacg atgaccacgc caatcgcaat agagatgaga tcttctggcg
     7321 tcactgccag taaatcaccg aacaggtaag ccatcaaatc aacacgaatg ttagacatca
     7381 gactaacgac cactaggccc agcgacaggg cactgtgcgc cataatccct aataacgtgt
     7441 cgatcgccag ctgtggacgc ttctccagcc ataccagacc gcccgccagc agcagcgtaa
     7501 cggcaatcac cgcatagaat ggattcacgt ccagcaacaa accaaacgcg acaccaagta
     7561 atgaggcatg agccagcgta tcaccgaaat aagacatacg acgccagact acaaacgaac
     7621 ccagcggacc cgcggcacag gcgagcatga tcccggctaa ccaaccggga aataataatt
     7681 caatcatgag cgatcatttc cccgacgcaa aacaattcgt ccctgtaaat cgtgacgatg
     7741 attatgatga tggcgataga tacccagttg ttcagcacca cgaggaccaa acattgaaat
     7801 aaactccgga tgcagggaaa caacttccgg tgtgccggaa caacaaatgt ggtgattcag
     7861 gcacagcact tcatcggttt ttgccatcac cagatgcaga tcgtgagaaa ccattaaaac
     7921 gccacaatcc agttcacgac gcaactggtc aataaggtca tataacgcca cttggccatt
     7981 tacatccacg ccttgagtgg gttcatccag cactaataat tgcgggcaat ttaacaatgc
     8041 tcgcgctaat agtacacgct gcgtttcgcc accagagagc ttttgcatcg gtgcgttaat
     8101 caggtgcccg gcctggacac gtttcagtgc aggcaaaata tcttctttat gtgtaccagg
     8161 gcgtaagcgt aaaaaacggt ttacggtcag tggcaacgtg gtgtcgagat acagcttctg
     8221 cggtacatag ccgatgcgca gttttccgtt gcgcttgata accccttcat cgggtgttac
     8281 cagcccgagc actacccgta ccagtgtcga cttacctgcg ccatttggcc caagtaaagt
     8341 caaaattttt ccaggtttaa gttccagcga cacatcagag aggacgcggc gttggccaaa
     8401 agaaaccgag acattttcca gggaaaccag acttgtcatg ttaattttag tcttgcagta
     8461 gtcatgaaat gttataatat cacacttctc atattcatta cgatgattgg tcgcattatg
     8521 ttacataaaa aaacgcttct tttcgcagca ttatccgccg ctctctgggg aggtgcaaca
     8581 caggccgcag atgccgccgt tgtcgcttcg cttaaacccg ttgggttcat cgcttctgcc
     8641 attgctgatg gtgtaacaga aacggaggtt ttactgcctg acggcgcttc agaacatgat
     8701 tattcactgc gtccatcgga tgtaaaacgc ttacagaacg cggacttagt cgtttgggtt
     8761 ggcccggaga tggaagcgtt tatgcaaaaa ccggtaagca aattaccaga agcgaagcag
     8821 gtaacgattg cgcagcttga agatgtgaaa ccgctactga tgaaaagtat tcacggcgat
     8881 gatgatgatc acgaccacgc ggaaaaaagt gacgaagatc accatcacgg cgatttcaac
     8941 atgcatcttt ggctttcccc agagatagcg cgggctacag cggttgcaat ccatggaaaa
     9001 ttagtggaac ttatgccgca aagtcgagcc aaacttgacg ccaacctgaa ggattttgag
     9061 gcacaattgg cctcaaccga aacgcaggtt ggtaacgagc tcgcgccgct caaggggaaa
     9121 ggttatttcg tttttcacga tgcttacggc tatttcgaaa aacagttcgg actgacaccg
     9181 cttggtcatt ttaccgttaa ccctgagatt caacctggcg cgcagcgttt acatgaaata
     9241 agaacacagt tggttgagca aaaagcaacc tgcgtttttg ctgagccaca gttcaggcca
     9301 gcggtcgttg agagcgtcgc acgagggaca tccgttcgta tgggaacgtt ggatcccctg
     9361 gggacgaata tcaaactggg taaaacaagc tattcagaat tcctgagtca attagccaac
     9421 cagtatgcga gctgcctgaa aggagattaa tgaggaagtg attacgtgca acagatagcc
     9481 cgctctgtcg ccctggcgtt taataattta ccgcgaccac accgcgttat gttggggtcg
     9541 ctcaccgttc ttactctggc cgtcgctgtc tggcggccct atgtttatca ccgtgatgcc
     9601 acgccaattg tcaaaaccat tgagctggaa cagaacgaaa ttcgttcgct cttacctgaa
     9661 gccagtgagc cgattgatca agctgcacaa gaagatgaag ccattcccca ggacgaactg
     9721 gatgacaaaa tcgccggtga agcgggcgtg catgaatatg ttgtttccac tggcgatacg
     9781 ctaagcagca ttctcaatca gtatggtatt gatatgggtg atatcaccca acttgctgct
     9841 gccgacaaag aattgcgtaa cctgaaaatc ggtcaacaac tctcctggac attaaccgcg
     9901 gacggcgaac tgcagcgcct cacctgggaa gtgtctcgtc gtgaaacccg aacctatgac
     9961 cgtactgccg ctaacggttt taaaatgacc agcgaaatgc agcaaggaga gtgggttaac
    10021 aatctgctga aaggtaccgt cggagggagc tttgttgcca gcgccagaaa cgccggttta
    10081 accagcgccg aagtgagcgc agtgattaaa gccatgcagt ggcaaatgga tttccgcaaa
    10141 ctgaaaaaag gcgatgaatt tgcggtatta atgtctcgag aaatgcttga tggtaaacgt
    10201 gagcaaagcc agctgctggg cgtacgtttg cgttcagaag gtaaagatta ttacgcaatc
    10261 cgcgctgagg atggcaaatt ctacgatcgt aacggtactg gtctggcgaa aggattcttg
    10321 cgattcccga cggcgaaaca gttccgtatc tcgtctaact ttaacccgcg tcgaactaat
    10381 ccggtgacag gtcgcgttgc accacacaga ggtgttgatt tcgccatgcc acaaggtacg
    10441 ccagtgcttt cagtgggtga cggtgaagtg gtggttgcca aacgcagtgg cgcagcaggt
    10501 tattatgtgg ctattcgtca tggtcgcagc tacaccacgc gttatatgca cttgcgcaag
    10561 attctggtga aaccgggaca gaaggtgaaa cgtggcgacc gtatcgcgct ttccggtaat
    10621 accggacgtt caactgggcc gcatctgcac tatgaagtat ggataaacca gcaggccgta
    10681 aacccgctga cggcaaaact gccgcgtacc gaagggctga ccggctccga tcgtcgcgaa
    10741 ttcctggcgc aggccaaaga gattgtgccg cagctacggt ttgattaatt aacatccatt
    10801 cgcagccggt acgcagtcag taccggcttt ttttatttgg tgcggggcaa gttgtgccgc
    10861 tacactatca ccagattgat ttttgcctta tccgaaactg gaaaagcatg gaaacgaaaa
    10921 aaaataatag cgaatacatt cctgagtttg ataaatcctt tcgccacccg cgctactggg
    10981 gagcatggct gggtgtagca gcgatggcgg gtatcgcttt aacgccgcca aagttccgtg
    11041 atcccattct ggcacggctg ggacgttttg ccggacgact gggaaaaagc tcacgccgtc
    11101 gtgcgttaat caatctgtcg ctctgctttc cagaacgtag tgaagctgaa cgcgaagcga
    11161 ttgttgatga gatgtttgcc accgcgccgc aagcgatggc aatgatggct gagttggcaa
    11221 tacgcgggcc ggagaaaatt cagccgcgcg ttggctggca agggctggag atcatcgaag
    11281 agatgcggcg taataacgag aaagttatct ttctggtgcc gcacggttgg gccgtcgata
    11341 ttcctgccat gctgatggcc tcgcaagggc agaaaatggc agcgatgttc cataatcagg
    11401 gcaacccggt ttttgattat gtctggaaca cggtgcgtcg tcgctttggc ggtcgtctgc
    11461 atgcgagaaa tgacggtatt aaaccattca tccagtcggt acgtcagggg tactggggat
    11521 attatttacc cgatcaggat catggcccag agcacagcga atttgtggat ttctttgcca
    11581 cctataaagc gacgttgccc gcgattggtc gtttgatgaa agtatgccgt gcgcgcgttg
    11641 taccgctgtt tccgatttat gatggcaaga cgcatcgtct gacgattcag gtgcgcccac
    11701 cgatggatga tctgttagag gcggatgatc atacgattga gcggcggatg aatgaagaag
    11761 tcgagatttt tgttggtccg cgaccagaac aatacacctg gatactaaaa ttgctgaaaa
    11821 ctcgcaaacc gggcgaaatc cagccgtata agcgcaaaga tctttatccc atcaaataaa
    11881 aaagcctctc gcgaggagag gccttcgcct gatgataagt tcaagtttgc ttcagaatat
    11941 tcgaaatctg ttgaactatc attgaactat aggccggatg tggcgttttc gccgcatccg
    12001 gcaacgtact tactctaccg ttaaaatacg cgtggtatta gtagaaccca cggtactcat
    12061 cacgtcgcct tgggtgacaa tcaccaggtc accagacatc aagtaacctt tatcgcgcag
    12121 cagattaacc gcttcgctgg cagctgctac gccgtcatta gcgctatcaa agggcaccgg
    12181 cgtaacgcca cgatagagag cagtcaggtt cagcgtacgt tcatggcgcg acatggcgaa
    12241 aattggtaga ccagagctga tacgggaggt catcagcgcg gtacgacccg attcggtcat
    12301 ggtaatgatc gccgtaacgc ccttcaggtg gttagctgcg tacattgccg acatggcaat
    12361 agcttcttcc acattgtcga actgaacgtc cagacggtgt ttagaaacgt tgatgctcgg
    12421 gattttttcc gcacccaggc aaacgcgcgc catggctgca acagtttctg acggatactg
    12481 cccagcggca gtttctgcag acagcatcac agcgtcagta ccatccagaa cggcgtttgc
    12541 tacgtccatg acttctgcac gcgtcggcat cgggttagta atcattgact ccatcatctg
    12601 ggtcgccgtg attaccgctc ggtttagctg acgcgcacga cggatcaacg ctttctgaat
    12661 gccgaccagt tccgggtcgc caatttccac accgaggtcg ccacgtgcaa ccattaccac
    12721 gtcagaggcg aggatgatgt catccattgc atcctggctg caaacggctt ccgcacgttc
    12781 aaccttggca acaattttcg catcacatcc tgcatcgcgt gccaggcgac gggcatagtt
    12841 cagatcttcg ccacagcgtg ggaaggagac agccaggtaa tctacgccaa tcaacgccgc
    12901 agtcttaatg tctgctttgt ctttttcggt cagcgcttca gccgacaaac cgccgccaag
    12961 tttgttgata cctttattgt tggagagggg accaccgacg gtgacttcgg tgaacacttt
    13021 catgccctga acttccagta cttttaactg gacgcgacca tcgtccagca gcaggatgtc
    13081 accaggcacg acgtcagtag gcaggccttt gtagtcgata ccgacttttt ctttgtcgcc
    13141 ttcaccttta cccaggttgg cgtcgagcag gaatttatcc ccaatattga ggaaaacttt
    13201 gccttcttta aaggtggata cacggatttt gggcccctgg aggtcaccca gaatagccac
    13261 atgacgcccc agttttgcgg caatctcacg aactttatcc gcgcgcattt tgtgatcttc
    13321 aggcgagccg tgagaaaagt tcatacgtac aacgttggca cccgccgcga taactttttc
    13381 aagattatta tcgcgatctg ttgctgggcc taacgtggta acgatttttg ttctgcgaag
    13441 ccttttggac atgtaatact ccgttgactg aaacaaccag gtgttgcttg aacatgaaat
    13501 ccgaatgaaa taacgccgcg atggcgataa attaatctca ttcggatgtc gtataagatt
    13561 aggacagtga cagtcgcttt tagcgatcgt cacttaaatt aagtaactgc ttatcaaaac
    13621 gcgattcctt cagcgcttcc ttgacccgct tcaagttatc tctgaatttt gcaccgcgtc
    13681 gcaaagtaaa tcctgtcgcc agcacatcta tcacggtcag ctgtgcaagt cgagaaacca
    13741 tgggcatata aatgtcagta tcttccggta cgtcgagggt aattgccagc gttgcttccc
    13801 gggcgagcgg ggtacccgca gaggtgaggg caatcaccat ggcgtcgttt tcgcgtgcca
    13861 gctgcgccag ctcgaccaga ttttttgttc ttcctgtgtg agaaatcagc accaccacgt
    13921 ctccgtcgct acaattcata caactcatgc gttgcagcac gatatcatcg gagtacacca
    13981 ccggaacatt aaaacgaaag aacttattca tcgcatcgtg ggcaacggcg gcagaagagc
    14041 ctaatccgaa aaaggcgatt ttttttgcct gagtgagcaa gtcgacggcg cggttgatgg
    14101 cagatttatc cagtgaatga cggacatgat cgagcgttgc cattgcggac tcaaatattt
    14161 tccccgtgta tgattcaacg ctgtcatctt cattgacgtt gcgattaaca tagggagtgc
    14221 cattcgccag actttgtgcc agatgaagtt taaaatcagg aaaaccgcgc gtgtccatgc
    14281 tgcgacagaa acgattcacc gtcggttcgc taacattggc ttccagcgcc agagcagcaa
    14341 tactcgaatg gatcgcgtta tcgggcgaag ccagaatgac ctcggcaact ttgcgctctg
    14401 atttgctcaa atgttccagc tgagactgga ttttttccag catattcatg atgtaaagag
    14461 actcacgggt aatgacgatt tccgcactga aagaaatcga aatgcagttt tgtcagatat
    14521 tacgcctgtg tgccgtgtta atgacaaaag cagataaaaa agttgttatt tttttcataa
    14581 catgatcagt gtcagatttt taccgaatgg aaaacgatga tttttttatc agttttgccg
    14641 cgctttgcgc gcttttcccg taatcgcacg ggtggataag cgtttacagt tttcgcaagc
    14701 tcgtaaaagc agtacagtgc accgtaagaa aattacaagt ataccctggc ttaagtaccg
    14761 ggttagttaa cttaaggaga atgacatggc ggtaacgcaa acagcccagg cctgtgacct
    14821 ggtcattttc ggcgcgaaag gcgaccttgc gcgtcgtaaa ttgctgcctt ccctgtatca
    14881 actggaaaaa gtcggtcagc tcaacccgga cacccggatt atcggcgtag ggcgtgctga
    14941 ctgggataaa gcggcatata ccaaagttgt ccgcgaggcg ctcgaaactt tcatgaaaga
    15001 aaccattgat gaaggtttat gggacaccct gagtgcacgt ctggattttt gtaatctcga
    15061 tgtcaatgac actgctgcat tcaaccgtct cggcgcgatg ctggatcaaa aaaatcgtat
    15121 cactattaac tactttgcca tgccgcccag cacttttggc gcaatttgca aagggcttgg
    15181 cgaggcaaaa ctgaatgcta aaccggcacg cgtagtcatg gagaaaccgc tggggacgtc
    15241 gctggcgacc tcgcaggaaa tcaatgatca ggttggcgaa tacttcgagg agtgccaggt
    15301 ttaccgtatc gaccactatc ttggtaaaga aacggtgctg aacctgttgg cgctgcgttt
    15361 tgctaactcc ctgtttgtga ataactggga caatcgcacc attgatcatg ttgagattac
    15421 cgtggcagaa gaagtgggga tcgaagggcg ctggggctat tttgataaag ccggtcagat
    15481 gcgtgacatg attcagaacc acctgctaca aattctctgc atgattgcga tgtctccgcc
    15541 atctgacctg agcgcagaca gcatccgcga tgaaaaagtg aaagtactga agtccctgcg
    15601 ccgcatcgac cgctccaacg tacgcgaaaa aaccgtacgc gggcaatata ctgcgggctt
    15661 cgcccagggc aaaaaagtgc cgggatatct ggaagaagag ggcgcgaaca aaagcagcaa
    15721 tacagaaacc ttcgtggcga tccgcgtcga cattgataac tggcgctggg ccggtgtgcc
    15781 attctacctg cgtactggta aacgtctgcc gaccaaatgt tctgaagtcg tggtctattt
    15841 caaaacacct gaactgaatc tgtttaaaga gtcgtggcag gatctgccgc agaataaact
    15901 gactatccgt ctgcaacctg atgaaggcgt ggatatccag gtactgaata aagttcctgg
    15961 ccttgaccac aaacataacc tgcaaatcac caagctggat ctgagctatt cagaaacctt
    16021 taatcagacg catctggcgg atgcctatga acgtttgctg ctggaaacca tgcgtggtat
    16081 tcaggcactg tttgtacgtc gcgacgaagt ggaagaagcc tggaaatggg tagactccat
    16141 tactgaggcg tgggcgatgg acaatgatgc gccgaaaccg tatcaggccg gaacctgggg
    16201 acccgttgcc tcggtggcga tgattacccg tgatggtcgt tcctggaatg agtttgagta
    16261 atatcagcgc ttatccttta tggttatttt accggtaaca tgatcttgca cagattgtag
    16321 aacaattttt acactttcag gcctcgtgcg gattcaccca cgaggctttt tttattacac
    16381 tgactgaaac gtttttgccc tatgagctcc ggttacaggc gtttcactca taaatcctct
    16441 gaatgaaacg cgttgtgaat catcctgctc tgacaactca atttcaggag cctttatgaa
    16501 tccacaattg ttacgcgtaa caaatcgaat cattgaacgt tcgcgcgaga ctcgctctgc
    16561 ttatctcgcc cggatagaac aagcgaaaac gtcgaccgtt catcgttcgc agttggcatg
    16621 cggtaacctg gcacacggtt tcgctgcctg ccagccagaa aacaaagcct ctttgaaaag
    16681 catgttgcgt aacaatatcg ccatcatcac ctcctataac gacatgctct ccgcgcacca
    16741 gccttatgaa cactatccag aaatcattcg taaagccctg catgaagcga atgcggttgg
    16801 tcaggttgcg ggcggtgttc cggcgatgtg tgatggtgtc acccaggggc aggatggaat
    16861 ggaattgtcg ctgctaagcc gcgaagtgat agcgatgtct gcggcggtgg ggctgtccca
    16921 taacatgttt gatggtgctc tgttcctcgg tgtgtgcgac aagattgtcc cgggtctgac
    16981 gatggcagcc ctgtcgtttg gtcatttgcc tgcggtgttt gtgccgtctg gaccgatggc
    17041 aagcggtttg ccaaataaag aaaaagtgcg tattcgccag ctttatgccg aaggtaaagt
    17101 ggaccgcatg gccttactgg agtcagaagc cgcgtcttac catgcgccgg gaacatgtac
    17161 tttctacggt actgccaaca ccaaccagat ggtggtggaa tttatgggga tgcagttgcc
    17221 aggctcttct tttgttcatc cggattctcc gctgcgcgat gctttgaccg ctgccgctgc
    17281 gcgtcaggtt acacgcatga ccggtaatgg taatgaatgg atgccgatcg gtaagatgat
    17341 cgatgagaaa gtggtggtga acggtatcgt tgcactgctg gcgaccggtg gttccactaa
    17401 ccacaccatg cacctggtgg cgatggcgcg cgcggccggt attcagatta actgggatga
    17461 cttctctgac ctttctgatg ttgtaccgct gatggcacgt ctctacccga acggtccggc
    17521 cgatattaac cacttccagg cggcaggtgg cgtaccggtt ctggtgcgtg aactgctcaa
    17581 agcaggcctg ctgcatgaag atgtcaatac ggtggcaggc tttggtctgt ctcgttatac
    17641 ccttgaacca tggctgaata atggtgaact ggactggcgg gaaggggcgg aaaaatcact
    17701 cgacagcaat gtgatcgctt ccttcgaaca acctttctct catcatggtg ggacaaaagt
    17761 gttaagcggt aacctgggcc gtgcggttat gaaaacctct gccgtgccgg ttgagaacca
    17821 ggtgattgaa gcgccagcgg ttgtttttga aagccagcat gacgttatgc cggcctttga
    17881 agcgggtttg ctggaccgcg attgtgtcgt tgttgtccgt catcaggggc caaaagcgaa
    17941 cggaatgcca gaattacata aactcatgcc gccacttggt gtattattgg accggtgttt
    18001 caaaattgcg ttagttaccg atggacgact ctccggcgct tcaggtaaag tgccgtcagc
    18061 tatccacgta acaccagaag cctacgatgg cgggctgctg gcaaaagtgc gcgacgggga
    18121 catcattcgt gtgaatggac agacaggcga actgacactg ctggtagacg aagcggaact
    18181 ggctgctcgc gaaccgcaca ttcctgacct gagcgcgtca cgcgtgggaa caggacgtga
    18241 attattcagc gccttgcgtg aaaaactgtc cggtgccgaa cagggcgcaa cctgtatcac
    18301 tttttaagac gacaaatttg taatcaggcg agagaaaact ctgatgaaaa actggaaaac
    18361 aagtgcagaa tcaatcctga ccaccggccc ggttgtaccg gttatcgtgg taaaaaaact
    18421 ggaacacgcg gtgccgatgg caaaagcgtt ggttgctggt ggggtgcgcg ttctggaagt
    18481 gactctgcgt accgagtgtg cagttgacgc tatccgtgct atcgccaaag aagtgcctga
    18541 agcgattgtg ggtgccggta cggtgctgaa tccacagcag ctggcagaag tcactgaagc
    18601 gggtgcacag ttcgcaatta gcccgggtct gaccgagccg ctgctgaaag ctgctaccga
    18661 agggactatt cctctgattc cggggatcag cactgtttcc gaactgatgc tgggtatgga
    18721 ctacggtttg aaagagttca aattcttccc ggctgaagct aacggcggcg tgaaagccct
    18781 gcaggcgatc gcgggtccgt tctcccaggt ccgtttctgc ccgacgggtg gtatttctcc
    18841 ggctaactac cgtgactacc tggcactgaa aagcgtgctg tgcatcggtg gttcctggct
    18901 ggttccggca gatgcgctgg aagcgggcga ttacgaccgc attactaagc tggcgcgtga
    18961 agctgtagaa ggcgctaagc tgtaaaagtc aaaatgcccg atctaggatc gggcattttt
    19021 gtagcgtttt tttgccgggt ttaaccctgt acttttacct gtccggcggc gtgcttcgcg
    19081 cgttcaatgg cgtcaacaac actctctgaa gtagccagta ccacccccag atgacggctg
    19141 ccatcaattt ccggcttacc aaataaacga atctgcaaat ctgcgcctac ggcattctgc
    19201 acattatcaa acgtgacatt ctgactggtc agttgtggca gaataacggc agaagctgca
    19261 ggaccatact gacggatccc gccaacagga agtccgagga aggcacgtac atgcagggca
    19321 aactctgaga gatcttgaga aattaacgtc accatcccgg tatcatgtgg acgaggggag
    19381 acctcactga aaatcacctc atcaccacag acaaacagct cgacaccaaa cagaccataa
    19441 ccgcccagtg ccagcaccac tttacgggca atctcctgcg cacgttcaag ggcaagcggg
    19501 ctcatttgct gtggttgcca ggattcacgg tagtcgccat cttcctggcg atgacctact
    19561 ggtgcacaga aatggacgcc atccaccgcg ctgacggtta gcagggtaat ttcgaagtca
    19621 aacttaacga cgccttcaac aattacgcgg cccgctccgg cgcgaccgcc ttgctgagcg
    19681 tattcccatg cctgagcaag ttgctctgca gagcgaataa acgtctgccc cttgccggaa
    19741 gagctcatca ccggttttac aatgcaggga tagccaatgg cggcaaccgc ctcgcggaaa
    19801 aggcttttgc tatcggcaaa acgataagtg gaagtgggca gctgcagctc ttctgccgcc
    19861 aggcgacgga taccctcgcg attcatcgtt aatttcgttg cgcgagcgca ggggacaaca
    19921 ttcagtcctt cctcttcaag ttggatcagc atatcggtgg caatagcttc gatctccggc
    19981 acgatataat gtggtttttc cagttcaacc acacggcgta atgcatcacc atcaagcata
    20041 ttaatgacat gggagcgatg cgcgacatgc atggctggtg cgtcggcata gcgatcgacg
    20101 gcaatcacct ctacgccgag acgctgacac tcgattgcca cttctttacc cagttcaccg
    20161 gagcctaata acatcacgcg agttgctgcc ggacgcagcg cagtgcctaa taacgtcata
    20221 acgaatatcc cctgattaat tccgcgcgca gtataaacga aaacgtttgc gtgtgtcttt
    20281 attgctgatg ttgatttctc aaccgaaaag aaatatactg tataaaatct cagttattat
    20341 gagaggctgc aaatggcggt tgaagttaaa tacgtagtca ttcgtgaggg cgaagagaaa
    20401 atgtcgttta ccagcaaaaa ggaagccgat gcttatgaca aaatgcttga tacggcggat
    20461 ctgcttgaca cctagctgac aaattctcca gtgcaaatgg aagacgagca acgtgaagcc
    20521 ctttcgctat ggctggcaga acaaaaagag gtgctgagca ccattctgaa aaccggcaaa
    20581 ttaccgtccc cacaggtcgt cggtgctgaa tccgaagagg aagatgcctc acacgcggcc
    20641 tgaagcaact tgcgctcctg acaatttgta ccatgctttc tcctgaatca ttgtgctatg
    20701 gagaaaaaca tgaaaaaaag aggggcgttt ttagggcggt tgttggtttc tgcctgcgca
    20761 tcagttttcg ctgccaataa tgaaaccagc aagtcggtca ctttcccaaa gtgtgaaggt
    20821 ctggatgctg ccggaattgc cgcgagcgta aaacgtgatt atcaacaaaa tcgcgtggcg
    20881 cgctgggcag atgatcaaaa aattgtcggt caggccgatc ccgtggcctg ggtcagtttg
    20941 caggacattc agggtaaaga tgataaatgg tcagtaccgc taaccgtgcg tggtaaaagt
    21001 gccgatattc attaccaggt ctgcgtggac tgcaaagcgg gaatggcgga atatcagcgg
    21061 cgttaatggc ttctgataat tcgattgacc gttggcggat gcggcgcgag tgccttgtcc
    21121 gtcccagaac gcaccacaat cgtaggcctg agaagacgca acagcgtcgc atcgggcaat
    21181 cagcatcaaa caatgccact caaaacgaca aactactttc acctaaaata cccttatcac
    21241 ctgatataca gtaaatacct tacctggcgc gaatttcttt gaataacggc ctaccgcccc
    21301 gcttatttgc gcatcttttg tctcgatata cattttctga catcctcatc cgctatgcta
    21361 caggcactaa aaatggttat tgaggagcaa atatggctaa ctggttaaat caactgcaat
    21421 ccctgcttgg gcaaagcagt tcttctacct cctcgtctgc ggatcaggga ttgggcaaac
    21481 tgttagtgtc aggcgcatta ggcggactgg ctgggctgct ggtcgcaaat aaatcagcac
    21541 gtaaacttct tacgaaatat ggcaccaacg cgttactggt tggcggcgga gcggtagcgg
    21601 gtacggtgct gtggaataaa tacaaagata aaattcgcgc ggcgcatcag gacgaaccgc
    21661 agtttggcgc gcaaagtacg ccgctggatg agcgtacgga acgtttgatc cttgcgctgg
    21721 tctttgccgc taaaagtgat ggtcatattg atgccaatga acgtgcggca atcgaccagc
    21781 aattgcgtga agccggcgtg gaagagcagg ggcgtgtact cattgagcag gcaatcgaac
    21841 aaccgctgga tccacaacgc ctggctaccg gggtccgcaa tgaagaagag gcgctggaaa
    21901 tctatttcct gagctgcgcg gctattgata ttgaccattt tatggaacgc agttacctga
    21961 atgcactggg tgacgcgctg aaaatccccc aggatgttcg tgaaggcatt gaacgcgatc
    22021 tcgaacagca aaaacgcact ttggcggaat aattccgctc tctggatagc ctgaagttga
    22081 ctatttcggg ctatccagaa ccgcacagcc atttgaacag gctgattttc tctgtctgca
    22141 taacattcga tatccgcgcc attacctgtc atcatctaag caatgacgac cctgtttcgc
    22201 ttgcatcccc ggtgagtttt gccaccctta taagatgttt ccaaccagaa agaacaataa
    22261 catgctacca aaagccgccc gcattcccca cgccatgacg cttcatggcg atacgcgcat
    22321 cgataattac tactggctgc gggacgatac gcgttctcag ccaaaagtcc tggactacct
    22381 gcaacaagaa aatagttacg gtcatcgggt gatggcctca caacaagcct tgcaggatcg
    22441 catcttaaag gaaatcatcg accgcattcc gcaaagagaa gtttctgcgc cctacataaa
    22501 aaatggctac cgctatcggc atatttatga accaggctgt gaatatgcta tctaccagcg
    22561 tcaatcggca ttcagcgaag agtgggatga gtgggaaaca ttgctcgatg ccaacaagcg
    22621 cgcagctcat agtgagtttt attcgatggg cggaatggcg attacgcccg ataacaccat
    22681 tatggcgctg gcagaagatt ttctttcccg acgccagtac ggcattcgtt ttcgtaatct
    22741 ggaaacaggt aactggtacc cggaactgct ggataacgtt gaacccagct ttgtctgggc
    22801 aaatgactcc tggactttct actatgttcg caagcatccg gtgacgctgc tgccttatca
    22861 ggtctggcgt cacgccatcg gtacgccagc atcgcaagat acactgatct acgaagaaaa
    22921 agacgatacc tattatgtca gcctgcataa aacgacgtcg aagcactatg tcgtcattca
    22981 tctggccagc gccaccacca gtgaagttcg cctgctggac gcggaaatgg ccgatgccga
    23041 gccgtttgtt tttctgccgc gccgcaaaga tcacgaatac agccttgatc actaccagca
    23101 tcgtttttat ctgcgttcca atcgccacgg caaaaacttt ggcttatacc gtacccgtat
    23161 gcgtgatgag caacagtggg aagagttaat tccgccacgc gaaaacatca tgctggaagg
    23221 gtttacgctg tttaccgact ggctggtggt tgaagagcgt cagcgcgggt taaccagttt
    23281 gcgccaaatt aaccgcaaga cccgggaagt cattggcatt gcctttgatg atccggccta
    23341 tgtgacctgg attgcctaca atccagaacc tgaaaccgcg cgattgcgtt atggttattc
    23401 ttccatgacc acaccagaca ctttgtttga actggatatg gataccggtg agcgtcgtgt
    23461 attaaaacaa acagaagtgc ctggttttga tgcggcgaat taccgcagtg aacacctgtg
    23521 gatagtcgcc cgtgatggcg tcgaagttcc ggtttcgctg gtctatcatc gcaaacattt
    23581 tcgcaaagga cacaacccgt tgctggtgta tggctatggt tcttacggcg caagtattga
    23641 tgccgatttc agttttagcc gcttgagttt gttagatcgt ggctttgtct acgccattgt
    23701 ccatgttcgc ggcggtggtg agctggggca acaatggtac gaagacggta aatttctgaa
    23761 gaagaaaaat acgtttaatg attatcttga tgcctgcgat gcattgttaa aactgggcta
    23821 tggctctcct tcgctctgtt atgcgatggg cggaagtgcg gggggcatgt tgatgggcgt
    23881 tgcaattaat caacgcccgg aattattcca cggcgttatc gcccaggtac cgtttgttga
    23941 tgttgtaaca acgatgcttg atgaatcaat tcctcttacc actggtgagt ttgaagagtg
    24001 gggaaatccg caggatccgc aatattacga gtacatgaaa agctacagcc cgtatgacaa
    24061 cgtcaccgca caggcttatc cgcatttact ggtaacgacc ggtttacacg attctcaggt
    24121 gcaatattgg gaaccggcaa aatgggtcgc taaattgcgc gagctgaaaa ccgatgacca
    24181 tcttttattg ctctgtaccg acatggactc aggccatggc ggtaaatctg gccgctttaa
    24241 atcgtacgaa ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc agggaacatt
    24301 acccgctacg cctgcggatt aagtattttc cagataatgt ttcagtgtta aacgcagctc
    24361 cgggctcatg ctgtccaggt tattaaataa ccagcgcaga tagcccggat agcgttcggc
    24421 aacgtcggaa accgctttgc cacggtattt gccaaaggtg aaggttgtca tcaacgacgg
    24481 acgtccggtg atatcggcca tctgttctgc cgtccagccg gaggtgttca taatatcgat
    24541 aagcaacgcg gcggtgatat aacagtcata caacgcgcgg tgatgatgca ggcccagcgg
    24601 tgtctgtaca ttgagcttgc gtgttttata taacgccata ttgctgtact tgagccctgg
    24661 ccacaaacga cgggccagtt tcatagtgca aatccactca ccgggcatct caggcagtac
    24721 gcggcggtca aagctggcgt tgtgcgcgac ataccattca ctaccgtaat agtgtgggat
    24781 cacatcttca atccacggtt tatcggcgac catggcttcg gtgatgcgat gaatcgccat
    24841 cgcttgtgga ctaataggac gatcggggcg caccaggagg ctcatggggt tgacgatttt
    24901 tccgtcaatg acatcaacag aggcaatctc aacgatccct ccctgcaaac cgcaggtttc
    24961 tgtatcgata atgcgcaaca tggcatgatc caggccgaaa aagcctagcg taatggaatg
    25021 atatcgcctt gccaaccctg tgatgaacgc tgaccgacta acaataatga accacgatcg
    25081 gcgcgaacga tgagacgacc atattcatcc catagtgcac tgttaccacg ggcatttgcc
    25141 atcagcacgg caatcgagta ctgatgtgaa aaaaattgta agcgacgggc cgacgccagc
    25201 aggtcgggtt ctcccagaca ttgccctgtc gtaaacagtg aacaggtcgg gtccatatcc
    25261 ataccttgcg gttgttcatc gaccaccgtg atggttctgg aacgacggcc cagacatgca
    25321 ccatgactct gatggtagtt cccaggcgtt tttcgccacg gtgcgaacac cgcaattcca
    25381 cgaataaagc gatcgttata ttcaacggga aggccggcaa tgatggtcat tcgctaggtc
    25441 gttgcggcat aacaaagcgg gtctaacagt gaaagatcgg gtggggcagg aagggcacgt
    25501 cgcgaataat cacaccccag taaagaaagc gatggaaaaa ctaacagctg acattgttgc
    25561 ctggcggcgg ccctcacaaa ctccagatga tgagctacct gttcggtgag tgaggtttta
    25621 cgaggctcat attgcgctgc tgcaactttc cagaacgaca taatgacttc cttttcaaaa
    25681 tgactgcaaa ttatgggggc ggtttagaaa atactcataa tttagcaggc gttatgtaag
    25741 aaagtgtaac tctgtataag ttttatttaa gtttgggctc gtaaggtaaa cgtgacagat
    25801 tgaccgaagc caaacggtgg gcaataagcc gctcgcgaaa ccagctgcgc aaatgttctg
    25861 gctgttcacg ttcaaccgct tcagcgatca ccggcatatt gtagcgttct ttaaaagcca
    25921 ccccggccgc cgccaaatcg acattcactt tatccatttc tgtttgatcc agtttagcca
    25981 gattcttcag cattcttaat ctcctcatca ttcgcgggca aaagttacta agagtgagcc
    26041 tggatggcaa gccggaattg atgatcctcg actcagacat tagacaaggt tatgctctgt
    26101 aaaaaagtta ttacaaaaag gaatagataa tatggcttca actgcacgct cccttcgcta
    26161 cgcgctggca atcctgacca cttcgttggt gacaccttcc gtctgggcac atgcgcattt
    26221 aacgcatcag tatcctgcgg caaacgcgca agtgacagct gccccgcagg caatcacttt
    26281 aaacttctcg gaaggcgttg aaacaggatt cagtggtgca aaaatcacgg ggccaaaaaa
    26341 cgaaaatatt aaaacattac cagcgaagcg gaatgagcag gaccaaaagc aactgattgt
    26401 tccactggcc gattcgctga aacccggaac ctacaccgtt gactggcatg ttgtttcggt
    26461 ggacgggcat aaaacgaaag gacactacac ctttagcgtg aaataaatca tgctggcgtt
    26521 tacctggatc gcgctgcgat ttatccattt cacctcgttg atgctggtct ttggctttgc
    26581 aatgtatggt gcctggctgg cccccttaat gattcgtcgc ttgctgacga agcgttctct
    26641 gcgtttacaa cagcacgctg ccgtctggag tttgatcagt gccactgcaa tgctcgccgt
    26701 tcagggcggg ttgatgggaa cgggatggac agatgtattt tcgccaaaca tttggcaggc
    26761 tgttttgcaa acgcagtttg gtggcgtctg gttatggcaa attgttctcg ccctcgtaac
    26821 gttgatcgtc gcccttatgc aaccgcggaa tatgccacgt ttgctgttta tgctcactac
    26881 cgcgcaattt attctgctgg cgggagtggg gtatgcgacg ctgaatgaag gggtaacagc
    26941 gaaaatccat cagactaatc acgcgattca cctgatttgt gcggcggcgt ggtttggggg
    27001 attactgccg gtgctctggt gtatgcaact catcaaaggc cgttggcgac actaggctat
    27061 tcaggcgctg atgcgttttt cctggtgcgg gcattttgcg gtgatcggcg tactggcaag
    27121 cggcgtgctt aatgcattgt tgattaccgg atttcccccc acgcttacga cttactgggg
    27181 ccaactctta ttgctcaaag ccatcctggt gatgattatg gtggtcattg cgctggctaa
    27241 tcgttatgtt cttgtaccgc gcatgaggca ggatgaagat cgtgcggcac cctggtttgt
    27301 gtggatgact aaactcgaat gggcgatagg tgcggtagtg ctggtgatca tcagcctgct
    27361 tgcgactctc gaaccttttt gatggattac gaaaatgatg aaaaaaagta ttctggcgtt
    27421 tctgttactc accagttctg cagcggcgct ggcggctcca caggtgataa ccgtgagtcg
    27481 ttttgaagtg ggtaaagaca aatgggcgtt taatcgcgaa gaggtaatgc tgacttgccg
    27541 accgggtaat gctttgtatg tcatcaaccc aagtaccctc gtgcagtatc ctttaaacga
    27601 tatcgcacaa aaggaagttg ccagtgggaa gaccaatgcc caacccattt cggtgattca
    27661 gattgatgat cctaacaatc ccggcgaaaa aatgagtctg gcaccgttta tagaacgagc
    27721 tgaaaaactc tgttaattac ctaaaatagc cttttgattt ccaataaaaa aaccgcctca
    27781 gttctttcac cagaacgggc ggtttttaac atttaagctg atgagcaccg cgctttttat
    27841 tgaccatttt gcacgcaaac tggaaaacct ggcgtcgtca tctattctta aagggcaagg
    27901 caactaagcc tgcattaatg ccaactttta gcgcacggct ctctcccaag agccatttcc
    27961 ctggaccgaa tacaggaatc gtattcggtc tttttttaat tgtatttaaa atcaattagt
    28021 tgcaaacgtc tccccgaaat tccccgaaat ttactcgaat ttctgtattc cggtcttttt
    28081 tggttatatc acaaccaaaa tacatttaac aatccattta cgttaaaatc agagcagtaa
    28141 gtacgttttt tctctctcat caagatacat ttttgttgtc ttctccgatg tgtggccaag
    28201 tagacgctga gcaaattctt ctccacatat ttctttgtac aatcgtccag ccaggcttct
    28261 gatttcgtga aaagttggtg ggttttcact gaacagtgat acctgttaat tttctggctg
    28321 cgacaaattt ctttgtcagg ccgtccgggt gaatgctgcc gtcagggctg ttttttctaa
    28381 tcccggcact gattagataa tcccccctac ttaccatgcg gcactgttca actactgcac
    28441 caagtcgtaa accagcgaca ggaaggctga gtgacagggg gatagcaatc atcattcctg
    28501 tcttaatttg cctgatgtgg agacgatcat cataaatatc actgaaccgc atattcgtta
    28561 tgtcttcgcg acgttgtcct gttacaaggg ctaagtccat agctaatggg aaccatgccg
    28621 gaagttgatc tgctgcctcc ctgatgcagt tgtatgtctt tagtttcagc cgttctcttg
    28681 taactactat tttcggtgct cttgttggcg ttactggatt ttgagatata cgtccttcaa
    28741 caatggcttc gcggaacata tcagataaca cagaacgcat tgatcctgcc atcgtgtttt
    28801 tccctccttc aatctacaaa tcaagaaact cggcgatatg gcgagtggtt atttctgtca
    28861 gtaaaacccc tcctaatttt tcttttattg tctccagttg atttacccga gttttataag
    28921 tatttctgga cgcttttctc cttataagaa tcgttttgta acgttcaatc cagcctgcca
    28981 tagtaaatga gtcgacccct ttaagctttt caattagggc agcaggagag tagtttttgt
    29041 atatataatg atttgcttca attgcctgcg ctactgcatc tcttcttgaa attttaccta
    29101 gtgtaaattc ttctttcgtc agagggttgc gccagtaata tgctttgtct ctccttcgat
    29161 atgttaagtt tctaggcaaa ttgggatcgt attttttccg ctgcatgttt taacttctcc
    29221 attaacggac tgtctctccc ttgtcgccca ttaggctgat ggtgtgttat atcggtatca
    29281 accttatttg ggttgatata gaaagcctcc ggaaccaccc tgtaactcct cccgtgtagt
    29341 tcaggtgcag gataaatgtt tccattcctt gcccatcgtc tcagcgttga tattgatggt
    29401 gggttatccg gatatctgag ttttccccac gttttgagtg tcacaagatt cattgccata
    29461 cctctcacga tatgaccgcc agtaaatata cagaatactg gcgggtgtgg ttgattttta
    29521 ata
//
LOCUS       DAJTRN010000045        29454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRN010000045 DAJTRN010000000
VERSION     DAJTRN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..197)
                     /gene="wcaD"
                     /locus_tag="OO328_002055"
     CDS             complement(<1..197)
                     /gene="wcaD"
                     /locus_tag="OO328_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5930628.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLT"
     gene            complement(172..1389)
                     /gene="wcaC"
                     /locus_tag="OO328_002056"
     CDS             complement(172..1389)
                     /gene="wcaC"
                     /locus_tag="OO328_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5930629.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1386..1874)
                     /gene="wcaB"
                     /locus_tag="OO328_002057"
     CDS             complement(1386..1874)
                     /gene="wcaB"
                     /locus_tag="OO328_002057"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5930630.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(1877..2716)
                     /gene="wcaA"
                     /locus_tag="OO328_002058"
                     /pseudo
     CDS             complement(1877..2716)
                     /gene="wcaA"
                     /locus_tag="OO328_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(2809..4971)
                     /gene="wzc"
                     /locus_tag="OO328_002059"
     CDS             complement(2809..4971)
                     /gene="wzc"
                     /locus_tag="OO328_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5930631.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(4974..5417)
                     /gene="wzb"
                     /locus_tag="OO328_002060"
     CDS             complement(4974..5417)
                     /gene="wzb"
                     /locus_tag="OO328_002060"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5930632.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5423..6562)
                     /gene="wza"
                     /locus_tag="OO328_002061"
     CDS             complement(5423..6562)
                     /gene="wza"
                     /locus_tag="OO328_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5930633.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            6871..7020
                     /locus_tag="OO328_002062"
     CDS             6871..7020
                     /locus_tag="OO328_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930634.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7221..8804
                     /locus_tag="OO328_002063"
     CDS             7221..8804
                     /locus_tag="OO328_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5930635.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9256..11109)
                     /gene="asmA"
                     /locus_tag="OO328_002064"
     CDS             complement(9256..11109)
                     /gene="asmA"
                     /locus_tag="OO328_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5930636.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11131..11712)
                     /gene="dcd"
                     /locus_tag="OO328_002065"
     CDS             complement(11131..11712)
                     /gene="dcd"
                     /locus_tag="OO328_002065"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5930637.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11804..12445)
                     /gene="udk"
                     /locus_tag="OO328_002066"
     CDS             complement(11804..12445)
                     /gene="udk"
                     /locus_tag="OO328_002066"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5930638.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12763..13005
                     /locus_tag="OO328_002067"
                     /pseudo
     CDS             12763..13005
                     /locus_tag="OO328_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13004..13945
                     /gene="mdtA"
                     /locus_tag="OO328_002068"
                     /pseudo
     CDS             13004..13945
                     /gene="mdtA"
                     /locus_tag="OO328_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            13945..17067
                     /gene="mdtB"
                     /locus_tag="OO328_002069"
                     /pseudo
     CDS             13945..17067
                     /gene="mdtB"
                     /locus_tag="OO328_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17068..20133
                     /gene="mdtC"
                     /locus_tag="OO328_002070"
     CDS             17068..20133
                     /gene="mdtC"
                     /locus_tag="OO328_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5930639.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20134..21549
                     /locus_tag="OO328_002071"
     CDS             20134..21549
                     /locus_tag="OO328_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5930640.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            21546..22949
                     /gene="baeS"
                     /locus_tag="OO328_002072"
     CDS             21546..22949
                     /gene="baeS"
                     /locus_tag="OO328_002072"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5930641.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            22946..23668
                     /gene="baeR"
                     /locus_tag="OO328_002073"
     CDS             22946..23668
                     /gene="baeR"
                     /locus_tag="OO328_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5930642.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            23859..24191
                     /locus_tag="OO328_002074"
     CDS             23859..24191
                     /locus_tag="OO328_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5930643.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24338..25699
                     /gene="yegQ"
                     /locus_tag="OO328_002075"
     CDS             24338..25699
                     /gene="yegQ"
                     /locus_tag="OO328_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5930644.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26041..26358)
                     /locus_tag="OO328_002076"
     CDS             complement(26041..26358)
                     /locus_tag="OO328_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930645.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            26764..27663
                     /gene="yegS"
                     /locus_tag="OO328_002077"
     CDS             26764..27663
                     /gene="yegS"
                     /locus_tag="OO328_002077"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5930646.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(27745..28518)
                     /locus_tag="OO328_002078"
     CDS             complement(27745..28518)
                     /locus_tag="OO328_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5930647.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(28624..>29454)
                     /gene="gatD"
                     /locus_tag="OO328_002079"
     CDS             complement(28624..>29454)
                     /gene="gatD"
                     /locus_tag="OO328_002079"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5930648.1"
                     /translation="GVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRD
                     GGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTI
                     GLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFN
                     QLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWM
                     NYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIARNAMQGKVLLIP
                     "
BASE COUNT         6740 a         7846 c         7671 g         7197 t
ORIGIN      
        1 gtgagtcccg cgccaatccc taaggcaatc atcaattttt taatgctgat gcgttctaaa
       61 aacagcagta acaagacagg taaaaaagtg acgatggtga tggggaaact ttcgccaagc
      121 tgggcgattt tgacgttaac cagcaggtag atcagtggca gcagcaggta gctacagatt
      181 ctgatagaag ttgacatact cctccagcat ctgttgtcca ctgtaggcgg cgcgactgcg
      241 ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc gcgatttccg gtttgcttaa
      301 ctgtaccagt tgcagcacct cttcttcact gacggtttta ccaccggatt tttgcagcac
      361 ttcccgcgcc gcatcgctat gggtggcaat caccggcacg ccaatcgata gcgcctcaca
      421 caaaatcagc gggtagttat cgacacgaga actgaacacc agcgcatcca tctgattgag
      481 cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga ttaaccacgt tgccagcggt
      541 gaacggcgag aacttaccaa aggtatgcag ttcgattttg tcgcccagcg ccatcatctc
      601 acgcaccagt tgctggttag ttttgccgtc gtaacgcagg tcatgcgcca ccactgcgat
      661 tttcggcttg ccctgagttt cgcgcaccgg aggcagatct gccagaatcg cttcggttgc
      721 catatcaatg ccattattga taatccggca acgccctggc ccgtacagac tattgaaagc
      781 gtcagccaca tgctggctgg gggaaataaa ctgacagccc agcgccagca tctcgcggaa
      841 taactggcgt ttgcccgcca ccagctggtg cgcgcgatca atcttcaccg gcggatagtt
      901 atttaaggtc gggcatttct ggcagcccgt tttccagcct tcgcaaccgt cggtaaaggc
      961 gcagcgtccg gttacactcc agtggtcgtg cagcgtccag accagggtga catccggttt
     1021 atggtttttc actttttcgc aaaagcgcac cacgctgtta agattcaacc agtagctgtg
     1081 cagcacatga aaatgcagga ccaccggacc cggtgtgcga gtaatggtgc gatataactc
     1141 attgaaattg ccgaacagat cgcgattaaa cagacgaaac aacgcaatgt tcgccatcgc
     1201 ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc tgatggctga cgctctcttt
     1261 gccgcctttg ccgtagccgt agacaaaatg tgacgccagc ccctgttgca gcgcacgctg
     1321 gtggagatct aacgccacac ctgctgcccc gccttccgcc agtcgcacat taaattgcag
     1381 aatattcatt taatcacctt cactcgcgct ttttctccca ccaccagcgc gttgtccggg
     1441 acagagtcga gcaccacgct gcccgcaccc acggtgacgt tgttaccaag cgtgatatca
     1501 ccaagaataa tgacgttggc accgagttcg acgccgttgc caatgtgtgg acatgccatg
     1561 ttatcggcac cccgattgcc gatagtgacg ccgtggcgaa tggtgaaatc atcccccgcc
     1621 accacgtttt tattgatcac tacggcgtaa ccgtgatgga tagtaaagcg gcggccaatg
     1681 gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg tgataatgcg atacagcacc
     1741 agcagcgggg ccgcccacag attgttgagg acgttctttt tgcgccacac cgaacaaaaa
     1801 tgagcgacgc gataggcaag taccatgcag cacgggcgta aactccagct gttggcgcgc
     1861 agatcttcca gcatctttag cgcccccgaa taccatcagc cagacgtttg ccgttacgca
     1921 ccgacagcag cgtcaacaag gtgcgccagg tcatacgttt attgcggatc tggtagaggg
     1981 taaacagctg atatttttta ctggcgcggt cgaatttgtc tttgtgcttg cggtaaaagt
     2041 ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg catttcgccg tgattgatat
     2101 gcaggatctg cgtcgcctct tccactttcc acggttcgcc gtactccacc accatccgca
     2161 ggaatatgtc gtaatcctgt gctgctttca gttcggtatc gaacaaacac tctttgaaac
     2221 gccatgccca ggtaaagacc tgattgccaa tgatattacg tttatagaac aggcggcgtg
     2281 agtacggcga tttgggatac agtggtaagc tcgccggttg ggaataaact tctccctagc
     2341 aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag ttgctgttta tgggcgagga
     2401 agacgctcag gcggttgggc gtccattcat catcatcatc gatcccggtg atgtattccc
     2461 cttgcgccag cataatcgcc tggttacgca ccgcgcacgc cccgctgtta atgtcgttgt
     2521 ggatgtaagt gatacgcgga tcgttgaggg cagtgacgta ctgttgcagt tgttcccagg
     2581 aggtggagca atcatccacg atgatcatct cccagttgct gtagtcctgg cgcaggaccg
     2641 attttatcgc ccgaatcgcc agttgttggc ggttccaggt tggcatatag attgagatca
     2701 gcggattgtc tttcataggt tgttctcccg gcttatgagc tagtttgctt gccggatgcg
     2761 gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc aggcgtcgtt atttcgcatc
     2821 cgacttatat tcgtattcgt aatagccata atcctgatac gcgctggcgc ggcggaagat
     2881 ggagttcaga atcacccctt tcaccggaat accgttttgc tcaaagcggc tcagactggt
     2941 ttccacttct ttcaatgtgt tgaccgcata acgcgccacc attaacgtgg ttccgacatg
     3001 acgaccaaca attgccgcat cggtcactgc cagaatcggc ggcgtatcaa tcaacaccag
     3061 gtcatagttt ttactcgccc agttcaccag ttcggcaaag cgttcgctca ttaacagttc
     3121 agaaggattt ggcggcacct gaccgcgcgg gatcaggtca aatttggcaa tagaggtcgg
     3181 tttagcagcg gtagtaatat cgccctgacc aatcagaatc tccgacagcc cattgacatt
     3241 attggtgccc aacagctcgt gggtgtagcc tttgcgcata tcgcagtcga tcaacaacac
     3301 gcgtttattg gtctggctga tcaccgccgc caggttggcg cagacaaagg ttttaccgat
     3361 tgacgggcta accccggtca tcatcaacac attgttctgc gcctgcatca tcgcgaagtg
     3421 cagactggta cgcagactac ggatggcttc aatcgccaga tcggttggat tccccactgc
     3481 cagtagctgg ctctgtttat agcgtttaac ccctttgatg gttttgacgc tatcgcgcgc
     3541 tttctgccac tccgacagcg ggatgctggc atagacgctg attccgtgtt cttccagcac
     3601 ttgcgggctt tcgatgccgc gattaaacaa cgagcgcagc agcacaccca cgatagagag
     3661 catcaggcca aggataatcg cgccgaggat aatcagccct ttcttcggtt ttagcacgcc
     3721 aggctgggtg attgccgggt caataatgcg cacatcgccg acggtgctgg cttcggtgat
     3781 tttcagctcc tgctctttat tcagcagttg catatagacc tgctgaccag actcgacatc
     3841 gcgggtcaga cggacaattt cctgctggtt tttcggcatc gccgttacgc gaccgttaag
     3901 tttggctttc tcttcttcca gcgcctgacg tttctccagc agcgtgcggt acgccgggtg
     3961 aactttggtg tacagcttgg agatttccgc ctctttaaag gtcagttcgt tcaactgcgc
     4021 gtcgatgttc accatcgaat cgagcaccgc tttcgcttca agcggcagat caacagaatc
     4081 tttatcctga cggaaggcat tcagtttgtt ttcggcaaca tcgaggcggc tacgtacttc
     4141 cggtaactgt tgcgcgagga aagcgaggct tttcgacgct tccgccgatt tgcgctcaat
     4201 attttgttcc tgatagttac gagcgatgct gttaagaatg tcgcggatct gttcgcgatc
     4261 ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg ccgttctccg ttaccgtcag
     4321 gctgttttgc agttggttga tcatccccaa cgtggagtat ttggtgacgg taaactcact
     4381 gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg ccttcttttt tcagcatctg
     4441 gcccgcttgc ccacgggcgc taaagccgcc atcactgctc agggtgtagt ttttgtcatc
     4501 cagcacatta agcgtaaaca cctgatccgc catctctttc gggcggttaa aggtagtcac
     4561 tttcaccgtc tcgttctgac gccccatcag gcgatcccag cccgccccga aaatcgggaa
     4621 cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc accgttttac caagcaccag
     4681 acgcgaacga atcaactgga tctcggcgtc cgatgcaggc ggtttgttgg ctaatgccga
     4741 gccgatatcc tgcaccagcg aattgccgct gttttgctcg atttgtacca gtgcgtcggc
     4801 actataaatc ggcgtggcga agaaagtgta aaccacggca cagagggcaa atacgacggt
     4861 gatgccaatc acccaccagc gcgcttcaat gacggtgccg accaggcgac caatatcgat
     4921 ttcatcactg cccgttaccg gagcggcatg ttgttttact ttttctgtca ttcttatacc
     4981 tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc gttcaagtaa tgtgtacacc
     5041 gctgcaaacg tttcccggct tttgtgatac ggatcgggga tttcacattc gttatcccag
     5101 tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca tctcgcataa gcgttcgata
     5161 tggcgcttat ccatggtcaa aatcaggtca tagttgcgac acaggcggcg gctgatttgt
     5221 cgtgcacagt ggccttccag agacagttga tgttctgcgg cgacgctgat agcggtagga
     5281 tcagcgccct taccgaccag cgcgccgagt ccagcggact ccactttcag ttccgggtga
     5341 taacgttgca gtaagcgttc cgccatcggg gaacggcaaa tattgccgac acagacaact
     5401 aagatgttgt taaacatgac gattaccagt tatgaatgtc gctggctgta tccgtcatgt
     5461 aacggacacc gctaatagtt ggcagcaact gattgatcag acggttccag cgggaaaccg
     5521 gggcggtggt gacatacacc acgtcataag gttgcaggcg gaattctgtc gccatcacca
     5581 gcgacgtcgc atcggacata tccagctggt agatattggc aatcttgccg ttacgcccgc
     5641 cctcgccttt cagcggacga atgacaaaga tgccgctggc gttggaggtg gtcatgtcga
     5701 taccttcggc attggccaga gcttcagtca gggtcatgcc gctaaagtcc attttcaggg
     5761 tgctctgttt cttcacttca cccatcacaa atactttcag atcatcatta cgcggcacgt
     5821 agagaatatc gccgggataa agcagtcggt tctgattgag gtcgccgttt tgcatcagcg
     5881 cctgtaaaga aatgcgttct tcacgaccat tgtgtgttag caccacgttg cgccagtcag
     5941 cggtgtcggt caggccacct gcggcgttga tggcgtcgag aatggtcagt ggtacgttgg
     6001 tgatcgcctg ttgaccggat ttattcacct gacctgagat ataggccttt tgtgagcgga
     6061 aggcggcgat attaacgtcc acctgcgggt cagcgatgta cgtcgctaag cgcccggtaa
     6121 tatcactgcg gatttcagcg agcgttttcc cgactacgtg aaccttgccg atatacgggt
     6181 aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc gctggagctg cggtactgac
     6241 cagctggcgt ggtgagttcc gggtgatccc agacggtgac attaagaacg tcccccggcc
     6301 cgacgcgata ctggtaattc gcgatctcgc tttccagcgt catattgggg cgcgctacat
     6361 tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag cggataaaca ttcaccattt
     6421 tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc tttgcccatc gtcgacatat
     6481 tgctgcccgg aagtactgtg caaccgctta tcaaggttac tgacaccaat aatggcatca
     6541 atttcatttt ggatttcatc attgtttatt tatcactttg gcagagtaat tatcctgtgc
     6601 actattaata gcaatgtcgc catgcacatt taccttgcag ttaattgaat aaaaatttaa
     6661 ctggcatcag tcctaaaaaa attgatttca tccgcaggct attgacagaa taattcagac
     6721 tggtctttca ggcatccaga cacgctaccg cccctggctt tttagctacc aatacactga
     6781 ttgagcttaa tttttcaaac cctctctgca tacaatcgtt gatgagaaag ggttattgcg
     6841 gaaattaact tccgaatata aggtgacatt atggtaattg aatattggct cgccaataat
     6901 gcaggaggaa gtgttacagc taacggaata gcaggcaagg taacaattcg gcaattggct
     6961 atttttaaga attatattaa gtatcattca atatggtttt taggagtttc tttaggttga
     7021 caatatttaa tattgcgtct ccacatgcga tatttcttaa ataatgtttt attattaccg
     7081 cttttaattc agggataatg tgaggttatt accctcaaat aatatgagat aaatagtgct
     7141 gcaacattgc atttttgccc cgatttatcc atgatcgaat tgtgacattt gtcatacaac
     7201 gaataggttt tgtacttact atggaatgga ttgccgatcc gtctatctgg gccgggttaa
     7261 tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa cctggtcttt attgccatcc
     7321 tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg ggttaccggg ctgctgctgg
     7381 cgatgttaat gcgcctgtta ctgctagcgt caatctcctg gctggtcacc ctgactcaac
     7441 cgctgttcag cttccgctcg tttaccttta gcgcccgcga cttaatcatg ctgtttggtg
     7501 gtttcttcct gttgttcaaa gccacgatgg agctgaacga acggctggaa gggaaagaca
     7561 gcaataatcc cacacaacgc aaaggtgcga agttctgggg cgtggtgacg caaattgtgg
     7621 tactggacgc cattttctca ctcgactcag tgattaccgc cgtcgggatg gtcgaccatt
     7681 tactggtcat gatggccgcc gtggttatcg ctatcagcct gatgttgatg gccagcaagc
     7741 cgttaacgca atttgttaac agtcacccga ccatcgttat tctctgctta agcttcctgt
     7801 tgatgattgg ctttagcctg gtggcagaag gtttcggctt cgtcattccg aaaggctacc
     7861 tgtacgctgc cattggtttc tcggtgatga tcgaggcact caatcagttg gctatcttta
     7921 accggcgacg ttttctttcc gctaatcaga cgctacgcca gcggactacc gaagcggtaa
     7981 tgcgcctgct tagcgggcaa aaagaagatg cggaactgga cgccaaaacc gcgtccatgc
     8041 tggtggatca tggaaaccag cagatcttta atccgcagga acggcggatg attgagcggg
     8101 tacttaatct taaccagcgt accgtaagca gcattatgac gtcgcgccac gatattgagc
     8161 atatcgatct caacgcgccg gaagaagaaa tccgccagtt gctggagcga aatcagcata
     8221 cgcggctggt ggttaccgac ggtgatgacg cagaagattt gctcggtgtt gttcacgtta
     8281 tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa cctgcgggtg ttgattcgcc
     8341 agccgctggt gttcccggaa accttgccgt tgttaccagc cctggagcag ttccgtaatg
     8401 cccgcacgca ctttgctttt gtggtggatg agtttggctc ggtggaaggg attgtgacat
     8461 taagtgacgt cactgaaacc attgccggta acttaccgaa cgaagtggaa gagatcgacg
     8521 cccgccatga tattcagaag aatgcggacg gttcctggac ggcgaatggt catatgccgc
     8581 tggaggatct ggtgcaatat gtgccgctgc cgctggatga aaaacgtgaa tatcacacca
     8641 ttgccgggct gttgatggag tatttgcagc gtattccaaa acctggcgaa gaagttcagg
     8701 tgggggatta tttgcttaaa acgttgcagg tggaaagcca tcgcgtgcag aaggtgcaga
     8761 ttataccgct gcgtaaggat ggcgagatgg agtacgaggt gtgatggtat cggccatttt
     8821 acccaatgct gcgtaacgcc ttataacttc agcaatgaag cgttgtctgt ggtaaacgga
     8881 catcacagcg ggggtgtggg tccggctgta aatcgccgaa tcgtttcctg taggctgggg
     8941 cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc gcgcccgtac
     9001 ccgatagccg aaaggaataa gatgagggaa ccgcgccagc ggcgatttac cgccgggagc
     9061 ccgggttgcc agggtggtgg cgattgagcc accctggcac gttcaccggc ttgagcgttc
     9121 catagtagcg aggaacatca agtgaacgga acagccttcg atcttacata ttcagaatat
     9181 aatagtgccg ccgcgaacgc ctgatgcgac gctttacgcg tcttatcagg cctacaggac
     9241 aggtcacctg gtaacctaca tcttctccag caacttcttc acatctttgc cattgcggga
     9301 atctttattc cgctcggccc agtcattcag gcgacgtttc gcttcgtcct gtagatgttt
     9361 gcgcagtaac tgatccactt gcaggctgta attgagttgc tgccagttgc cataaacccg
     9421 cagcggtact ggcgtttctt tgaggaaatc aatcagtttg ctttccccgt tccagccacc
     9481 cacgacccga atatcaaact gggtgtcgca ggtttgatct gccagattca acatgccttc
     9541 ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg tttaacgtca cgacgccatc
     9601 cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc acgttatcga agttttcagc
     9661 ggccttcaca tcaccgccat tacgctctac cgcttgctga atcatctgct ggaagttcat
     9721 cccttccata cgtgtgtcgg tcatctcaac atgcgcctgt ccttgccagt tgtggcggaa
     9781 tgcgtcggca tctatgtcag caccggagaa atcaccagcc agtgacattt ttccggtcaa
     9841 cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc tcaacgttct ccagccgtgg
     9901 ctggaagttt atccgcggat ttattgatgt cgcgtccagc gtgcccggca gtgaaacctt
     9961 tccaccgtta agcttgccct gcagttgagt aatttccagc aaacccgact tgttggtcat
    10021 ttgcgtggca acatctgtaa aattcattcc gcgccagcgc acgttactgg cctgcaacaa
    10081 aatatcagct gtaaagcctt gcagtccctg ataggccggt tcatcaatac gcgaagaaat
    10141 gaccgggcgc ggcaacgttg attggctctg cccctgctgc gcggcaccgt tttcaccatt
    10201 cgcggtttca ttaagcggga tgaggttgtc aagattcagt tgcgggaatt gcagtctcag
    10261 ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgt ccgctcagcg tactgtcatt
    10321 ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt gactcctgcc actgggactg
    10381 gaaactcccc tgcccctgaa taccttgttt tggtaaatcg gcaccctgca actgccagtt
    10441 aatttgttca atagccgctg ttaaatcatg cggataatca gaagcatcta ccgtaccgtt
    10501 aagggatatc gtgagatcgc gctgatcgcg attaacccgc ccggagaact caaatgagcc
    10561 acgatgttgg ggatcttgtt ccatttgcag gcggatattg cggattgtca cctgctcgtc
    10621 atcttcatgc tggaacacca gaacgctgtc cgccacctta agactggata tatcaaacga
    10681 ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg cgcggtgcaa ccggagcgtc
    10741 ttcactgcgc accgcttccg tctgcggcgt cagttggatc actgcccctt ttagcatcac
    10801 ctgcttaacg ctcagttgat gactcagtag tggtaaaagc gccacgtcca gacgcatgtt
    10861 gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg agagacattc gcccggagag
    10921 gatactaagc tgcggccaga cgtgccaacg cagtggcccg tcgagctgca attgataacc
    10981 gctacgtgca gcaacttgct tgaccatata gtcgcggaaa tcattcggat tcaccagcaa
    11041 cactaacgca gataacccgg ccaccagcac gaccaagagt atcatcagcg tcgtcagaaa
    11101 tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta tcgattcggc tggctaccgc
    11161 gccctgctgg ttgcgatatt tcgcatcttc acggcggttg taaggtcgcg ccgccgggcc
    11221 ggaaagcggc tcaaagctca gcgcaccaat taacatgccc ggacgcagcg ccagcggcag
    11281 cttaccggag ttgtagaact ccagcacaat gcaaccagac cagcccggat cgatacggtg
    11341 cgcggtgacg tgcaccatca gccccagacg cgccaaagag gaacgcccgt ccagccagcc
    11401 caccagatcg gctggcagcg tcaccgactc cagtgtcacc gccagcgcca gctctcctgg
    11461 gtgaagatag aacgcctcgc cctcgtcgag aacgatctca tcgctcatca cgcggtcaag
    11521 cgcggcgctc acttcatctt tgggaccgct cagatcgata aacgctgccg tgtgaccacg
    11581 gaaggtacga aatttattgc ccaggcgtac atccaccgtc gcgccgttaa tacgctccac
    11641 tggcggacgt gggttgatcg acaaacggcc ttcatcaagc caggcttcaa tatctcggtc
    11701 acacagacgc atggcatttt ctcctttcgc acatcactcc cttaacgcca attacgtcaa
    11761 gggcatacca acttatcact gaacggtaca caatttatca agcttattca aagaactgac
    11821 ttattttcgc tttcaatata tcgatcgcga tgcggttttt cccgccgcgc ggcacgataa
    11881 tgtccgcata ttgtttagaa ggctcaatga attgcaggaa catcgggcgc acggtttttt
    11941 gatattgcgc catcacggaa tccattgaac gcccacgctc gttaacgtca cgcttgatgc
    12001 ggcgcatcag gcagatatcc agcggggtat caacgaaaat ggagaagtta agttcgtcac
    12061 gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat gatgaccttc ttcggctcaa
    12121 ccgtcaccgt ttctttcata cgcgtatgtt caacatagct gtaaaccggc aggtcaattg
    12181 ccgagccgcg tttcaacgct tgtaaatgct caagcaacag gctgtgatcc atcgcgctgg
    12241 gatggtcgta gttggtctta acgcgttctt ccatcgacag atggctttga tctttgtaat
    12301 agcagtcttc gggaattacg ccgatgtgtt catcaccgac ttgctcacgt aattcacgat
    12361 aaagggtact ggcaataaga ctcttgccgg aagccgatgc gccagcgata ccgataatga
    12421 cgcactgatg agactgatca gtcatatatt tagcgacctg attaacctgg atgttaggaa
    12481 gggggcgacg aagcgccaaa cgcggcaatt atagggattt catccgtctg ataccagtcg
    12541 aatagcgttg ccgcgcgctc agagttaatt gttgacaaag aattcccggt ggcaaattac
    12601 gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag ttgcgcaaat tatccgcctt
    12661 tacgtcactt tatgagcaat tcgcatataa aatgtaaaac ttttgtacta gcataaacac
    12721 agaaacgaat actggcgacc tggccttgcg gataaagcgg taataagcaa acaatcacag
    12781 catgtattaa ttgccctgcc ccacccgctg cttcacctgg tcagtttagg tttagtctcg
    12841 tttatcttta cccttgtctc gcttgagctt tcgcagtttg gcacccaact cgccccactg
    12901 tggttcccga cgtccatcat gatggtggcg ttttatcgcc atgccgggcg catgtggccg
    12961 ggaattgcgc tgagctgttc gctgggaaat atcgccgcat ccattccagg aaggccagca
    13021 ggtcaaagca ggcgatttac tggcagaaat tgaccccagc cagttcaaag ttgcattagc
    13081 acaaacccag ggccaactgg caaaagataa agccacgctt gctaacgccc gccgtgatct
    13141 ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc cgtcaggagc tggatgccca
    13201 acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag
    13261 cgcgcagttg caactcgact ggagccgcat caccgcacca gtcgatggtc gcgttggtct
    13321 caagcaagtt gatgttggta accaaatctc cagtggtgat accaccggaa ttgtggtgat
    13381 cacccagacg catcctatcg atttagtctt taccctgccg gaaagcgata tcgctactgt
    13441 agtgcaggcg caaaaagccg gaaaaccgct gatggtagaa gcctgggatc gcaccaattc
    13501 gaagaaatta agtgaaggca cgctgttaag cctcgataac caaatcgatg ccacaaccgg
    13561 tacgattaaa gtgaaagcac gctttaataa tcaggatgat gcgctgtttc ccaatcagtt
    13621 tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc caacagccgc
    13681 cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa acaaggtcag
    13741 caaacatctg gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc gtgcaggtat
    13801 ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt
    13861 ggaagtggtg gaagcccaga gcgccaccac tccggaagag aaagccacca gccgcgaata
    13921 cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc cgagcagcac aggcggcccg
    13981 tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc tgatggtggc gatcttactc
    14041 gccgggatta tcggttatcg cgccctgccc gtttcggcgc tgccggaagt ggactatccg
    14101 accattcagg tggtcacgct ctacccaggt gccagcccgg atgtcatgac ctctgccgtt
    14161 accgcgccgc tcgaacgcca gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa
    14221 agttccggcg gtgcgtcagt tatcactttg cagttccagc taacattacc gctcaatgtc
    14281 gccgagcagg aagtgcaggc cgcgattaac gctgcgacca acttgttgcc gagcgatctg
    14341 cctaacccgc cggtttacag caaagtgaac ccggcagatc cgccgatcat gacgctcgcc
    14401 gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata tggtggaaac ccgcgtcgcg
    14461 cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg tcagcgtccg
    14521 gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc tcggcctgac cagcgaaacc
    14581 gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa aaggtagcct cgacggccct
    14641 tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat ccgccgaaga gtatcgccag
    14701 ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg gcgatgtcgc aactgtagag
    14761 caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca aagaacaggc cattgtgatg
    14821 aatgttcagc gccagcccgg tgctaacatt atctccaccg ccgacagcat tcggcagatg
    14881 ctgccacagc tcactgagag tctgccgaaa tcggtgaagg tgacagtgct ttccgatcgc
    14941 accaccaata tccgcgcatc cgtcgatgat actcagtttg aattgatgat ggctatcgcg
    15001 ctggtagtca tgattatcta cctgtttttg cgcaatattc cggcgaccat cattcccggt
    15061 gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta tggtgtttct cgatttttca
    15121 atcaataacc tgacactgat ggctttaact atcgccaccg gattcgtggt cgatgacgcc
    15181 atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg
    15241 gcgctcaagg gcgcaggtga aatcggcttt accattatct cgctgacctt ctcactgatt
    15301 gcggtgttga tcccactgct gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt
    15361 gctattaccc tggcggtagc gattttgatc tcagcggtgg tgtcgctgac cctgacaccg
    15421 atgatgtgcg cgcggatgct cagccaggag tcgttgcgta aacagaacag cttctcccgt
    15481 gcctcggaaa aaatgttcga caggataatc gccgcctatg gtcgtggact ggcgaaagtg
    15541 ctgaatcatc cgtggctgac cttaagcgtg gcactcagca cgctgctgct tagcgtgctg
    15601 ctgtgggtgt tcattccgaa aggtttcttc ccggtacagg acaacggcat tattcagggc
    15661 actttgcagg caccgcaatc cagctccttt gccaatatgg cccagcgaca acgccaggtc
    15721 gcggacgtga ttttgcagga tccggcagtg caaagcctga cctcatttgt tggcgttgat
    15781 ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca acctcaaacc gttggatgaa
    15841 cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtaga taaagtgccg
    15901 ggcgtcgatc tcttcctgca accaacgcag gacctgacca tcgatactca ggtcagccgc
    15961 acccagtacc agtttacctt gcaggccacg tcactggatg cgctcagtac ctgggtgcca
    16021 cagttgatgg aaaaactcca gcaactgcca cagctttctg atgtctccag cgactggcag
    16081 gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc
    16141 agcatggcgg acgtcgataa cgccctgtac aacgcgtttg gtcagcgact gatttccact
    16201 atttatactc aggctaacca gtatcgcgtg gtgctggagc acaataccga aaatacccca
    16261 ggcctcgcgg cgctggatac cattcgcctg accagcagcg acggtggcgt ggtgccgtta
    16321 agctcaattg ccaaaattga gcagcgtttt gcgccgcttt ccatcaacca tctggatcag
    16381 ttcccggtaa caaccatctc ctttaacgtg ccggataact attcgctggg cgatgcggtg
    16441 caggcgatta tggacaccga aaagacgctg aatctgccgg tggatatcac cacgcaattc
    16501 tagggcagca ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg
    16561 gcggtggtgg cgatgtatat cgtgctcggc attctgtacg agagctttat tcacccgatc
    16621 accattctct cgacgctacc caccgcaggg gtcggcgcgc tgctggcatt aatgattgcc
    16681 ggtagcgaac tggatgtgat tgcgattatc ggcattattt tgctgatcgg tattgtgaag
    16741 aagaacgcca tcatgatgat cgacttcgcg ctggctgctg agcgcgagca aggcatgtcg
    16801 ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc gtccgatcct gatgaccacc
    16861 ctggcggctc tgcttggcgc gctgccgctg atgttgagta ccggggtcgg cgcggaactg
    16921 cgtcgtccgt taggtatcgg catggtcggc ggtctgattg tcagccaggt gctgacgctg
    16981 ttcaccacgc cggtgattta tctgctgttc gaccgcctgg cattgtggac caaaagtcgc
    17041 tttgctcgcc atgaagagga ggcgtaagtg aagttttttg ccctcttcat ttaccgcccg
    17101 gtggcgacga ttttactgtc ggtcgccatt accctgtgcg gcatactggg tttccgtatg
    17161 ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga ttatggtcag cgcctcgctg
    17221 cccggtgcgt cgccggagac aatggcgtct tccgttgcca cgccgctgga gcgctcactt
    17281 gggcgcattg ccggggttag tgaaatgacc tccagcagtt cgctcggcag cacgcgcatt
    17341 attttgcagt ttgattttga ccgggatatc aacggcgcag cgcgtgatgt gcaggcggcg
    17401 atcaacgctg cacaaagttt gctgcccagc gggatgccca gccgcccgac ctatcgcaaa
    17461 gcgaacccgt cggatgcgcc aattatgatc ctcacgctga catccgatac ttattcgcag
    17521 ggtgaactgt acgatttcgc ctcgacgcag ctggctccga cgatttcgca aatcgacggt
    17581 gtcggtgatg tcgatgtcgg tggcagctcg ctgcccgccg tgcgcgtcgg gctgaccccg
    17641 caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac gcactgccat cagcaatgcc
    17701 aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc accgctggca gatccagacc
    17761 aacgatgaac tgaaaaccgc cgctgaatat cagccgctga ttattcacta caacaacggc
    17821 ggcgcggttc gtctgggcga tgtggcgacg gtgactgact cagtgcagga tgtgcgcaac
    17881 gccgggatga ccaacgccaa accggctatt ttactgatga tccgcaaact gccggaagcc
    17941 aatattatcc agacggtaga cagcatccgg gcaaaattac cagagttgca ggagaccatt
    18001 ccggcggcga ttgatctgca aattgcccag gatcgctccc ccaccattcg cgcctcgctg
    18061 gaagaagtcg agcaaacgct gattatctcg gtggcgctgg tgattctggt ggtcttttta
    18121 ttcctgcgct cgggtcgcgc cactattatt cccgccgttg cggtgccggt ttcgctgatt
    18181 ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca ataatctttc gctgatggcg
    18241 ctcaccattg ctactggttt cgtggtggat gacgccattg tggtgctgga aaacattgcc
    18301 cgtcatctgg aagcggggat gaaaccacta caggccgcgc tgcaaggtac tcgcgaagtc
    18361 ggctttacgg tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc gctgctgttg
    18421 atgggcggat tgccgggccg actgttacgc gagtttgccg tgacgctttc tgtcgccatt
    18481 ggtatttcgt tgctggtttc cctgacgcta acgccaatga tgtgcggctg gatgctaaaa
    18541 gccagcaagc cgcgtgagca aaagcgactg cgtggttttg gccgcatgtt ggtagccctg
    18601 caacaaggct acggcaagtc gctgaaatgg gtgctcaatc atacccgtct ggtgggcgtg
    18661 gtgctgcttg gcaccattgc gctgaatatc tcgatcccga aaaccttctt cccggagcag
    18721 gacaccggcg tactgatggg tgggatacag gcggatcaga gtatttcgtt tcaggcgatg
    18781 cgcggcaaat tgcaggattt catgaaaatt atccgtgacg atccggcagt ggataatgtc
    18841 accggcttta ccggcggttc acgagttaac agtgggatga tgtttatcac cctcaagcca
    18901 cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc gtctgcgcgt gaagctggcg
    18961 aaagaaccgg gggcgaatct gttcctgatg gcggtacaag atattcgcgt tggtgggcgt
    19021 cagtcgaacg ccagctacca gtacacgttg ttatccgacg acctggcggc actgcgcgaa
    19081 tgggagccga aaatccgcaa aaaactggcg acgttgccgg aactggcgga cgtgaactcc
    19141 gatcagcagg ataacggcgc ggagatgaat ctggtttacg accgcgacac catggcacgg
    19201 ctgggaatcg acgtacaggc cgccaacagt ctgttaaata acgccttcgg tcagcggcaa
    19261 atctcgacca tttaccagcc gatgaaccag tataaagtgg tgatggaagt ggatccgcgc
    19321 tatacccagg acatcagtgc gctggaaaaa atgttcgtta tcaataacga aggcaaagcg
    19381 atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg ccccactatc ggtgaatcat
    19441 cagggattat cggcggcctc gaccatctcg ttcaacctgc cgaccggaaa atcgctctcg
    19501 gacgccagtg cggcgatcga tcgcgcaatg acccagcttg gtgtgccttc gacggtgcgc
    19561 ggcagttttg ccggcacggc gcaggttttc caggagacga tgaactcgca ggtgatcctg
    19621 attatcgccg ccatcgccac ggtgtatatc gtgctgggta tcctttacga gagttacgta
    19681 catccgctga cgattctctc caccctgccc tcggcgggcg ttggagcgct gttggcgctg
    19741 gagctgttca atgccccgtt cagcctaatc gccctgatag ggatcatgct attaatcggc
    19801 atcgtgaaga aaaacgccat tatgatggtc gattttgcgc ttgaagccca acggcacggt
    19861 aacctgacgc cgcaggaagc tattttccag gcctgtctgc tgcgttttcg cccgattatg
    19921 atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg tattgtcggg cggtgacggc
    19981 tcggagctgc ggcaacccct ggagatcacc attgtcggcg gtctggtgat gagccagctc
    20041 ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg accgtctgcg gctgcgtttt
    20101 tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag atcttcccga cagcacccgc
    20161 tggcaattat ggattgtggc tttcggcttc tttatgcagt cgctggacac caccatcgta
    20221 aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa gtccgttgca tatgcacatg
    20281 gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc ccgccagcgg ctggctggcg
    20341 gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg tgctgtttac tctcggttca
    20401 ctgttttgcg cgctttccgg cacgctgaac gaactgttgc tggcacgcgc gttacagggc
    20461 gttggcggtg cgatgatggt gccggtcggc aggttgacgg tgatgaaaat cgtaccgcgc
    20521 gagcaatata tggcggcgat gacctttgtc acgttacccg gtcaggtcgg tccgctgctc
    20581 ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt ggcactggat ctttttgatc
    20641 aacattccgg tggggattat cggtgcgatc gccacattga tgttaatgcc gaactacacc
    20701 atgcagacgc ggcgctttga tctttccggc tttttattgc tggcggttgg catggcggta
    20761 ttaaccctgg cgctggacgg cagtaaaggt acaggtttat cgccgctggc gattgctggc
    20821 ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc tgcacgccag aaataacaac
    20881 cgcgccctgt tcagtctgaa actgttccgc actcgtacct tttcgctggg cctggcgggg
    20941 agctttgccg gacgtattgg cagtggcatg ttgcccttta tgacaccggt tttcctgcaa
    21001 attggcctcg gtttctcgcc gtttcatgcc ggactgatga tgatcccgat ggtgcttggc
    21061 agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc gctttggtta tcgtcgggta
    21121 ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt tgtttatgac taccgctctg
    21181 ttgggctggt actacgtttt gccgttcgtc ctgtttttac aagggatggt caactcgacc
    21241 cgtttctcct ccatgaacac cctgacgctg aaagatctcc cggacaatct ggcgagcagc
    21301 ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga gtatcggcgt caccatcgcc
    21361 gggctgttgc tggggctttt tggttcgcag catatcagcg tcgacagcgg caccacacaa
    21421 accgtcttta tgtacacctg gcttagcatg gcgtttatca tcgcccttcc ggcgttcatc
    21481 tttgccagag tgccgaacga tacgcatcaa aatgtagcta tttcgcggcg aaaaaggagc
    21541 gcgcaatgaa gttctggcga cccggtatta ccggcaaact gtttctggcg attttcgcca
    21601 cctgcattgt cttgctgatc agtatgcact gggcggtgcg tatcagtttt gagcgtggct
    21661 ttattgatta catcaaacac ggcaatgaac agcgattaca gctgttaagt gatgcgcttg
    21721 gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg caacaacgat cgctttgtct
    21781 ttcagatcct gcgttcattt gaacacgata attcggaaga taaacccggc ccgggtatgc
    21841 cgccgcacgg ctggcgtacc cagttctggg tggtcgatca aaacaacaaa gtactggttg
    21901 gtccgcgagc gccgattcca cctgacggta cacggcgacc cattctggtc aacggtgcgg
    21961 aagtgggcgc ggtgattgcc tcccctgttg agcggctgac ccgcaatact gatatcaatt
    22021 tcgataaaca acagcggcaa accagctggc tgatcgtcgc cctggcaacg ttactcgcgg
    22081 cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc ggtaaaacga cttgtcgatg
    22141 gcacgcacaa actggcggcg ggcgatttca ctacccgcgt aacgcccacc agtgaagatg
    22201 aactgggcaa actggcgcaa gacttcaacc agctcgccag cacgctggag aaaaaccagc
    22261 aaatgcggcg cgattttatg gccgatattt ctcacgaact gcgtacgcca ttagcggtgc
    22321 tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa attcacgccg gagacggtgg
    22381 cgtctttaca ggcggaggtc ggtacactga ccaaactggt tgacgatctt catcagttgt
    22441 cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc ggtagatttg atcccactgc
    22501 tggaagtggc gggcggcgca tttcgcgaac gattcgccag ccgtggcctg aaactgcaat
    22561 tttccctgcc agacagtatc accgtatttg gcgatcgcga ccgtttaatg cagttattca
    22621 ataacttact ggaaaacagc ctgcgctaca ctgacagcgg cggcagcctg aaaatctctg
    22681 ccgagcagca cgacaaaacg gtgcgcctga cctttgccga cagtgcgcca ggtgtcagtg
    22741 acgatcagct acaaaaattg tttgaacgtt tttatcgcac cgaaggttcc cgcaaccgcg
    22801 ccagcggcgg ttccgggctg gggctggcga tttgcctgaa cattgttgaa gcccataatg
    22861 gccgcattat tgccgcccat tcgccttttg gcggggtaag cattacagta gagttaccgc
    22921 tggaacggga tttacagaga gaagtatgac cgagttacca atcgacgaaa acacaccgcg
    22981 tattttgatc gtggaagatg aaccgaagct ggggcagttg ctcattgatt atctgcgtgc
    23041 tgcgagctat gcgccgacgc ttatcagcca cggcgatcag gtactggcgt atgtgcgcca
    23101 gacaccaccg gatctgatcc tgttggatct gatgctccct ggcaccgatg gcctgacatt
    23161 atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg atggtgacgg caaaaatcga
    23221 agagatcgac cgcctgctgg ggctggagat tggcgccgat gactacatct gcaagccgta
    23281 cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg cgccgttgta aaccgcaacg
    23341 cgaacttcag caacaggatg ccgaaagccc gttgattatc gacgaaggtc gttttcaggc
    23401 ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa tttcgtctgc ttaaaacgct
    23461 ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg ctcaatcatc tttatgacga
    23521 ctaccgcgta gtaaccgacc gcaccattga cagccacatt aaaaatctgc gccgcaagct
    23581 ggaatctctc gacgccgaac agtcatttat ccgcgccgtt tacggcgttg gttaccgctg
    23641 ggaagccgac gcctgccgca tcgtttagtt ttagcgacat tactttgtta gccggagatg
    23701 gcgttcagac ccgatccggc aacattattc cacgcatggt cagcaaactg acattatctc
    23761 tccgccctgg caggcatcag aaaatcgctc atactttaat cagtaaacag cacctttaga
    23821 tgctgttttc gataagcaat ttcaatcaag gagtcattat ggctggttgg tttgaactca
    23881 gtaagagcag tgataatcag ttccggtttg tgctaaaagc gggcaatggt gagactatcc
    23941 tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa gggcatcgcg tcggtgcgta
    24001 gcaacagccc gcaagaagag cgctatgaga aaaaaacggc aagcaacggc aaattctatt
    24061 tcaatctgaa agccgctaat catcaaatta tcggctccag ccagatgtac gccaccgcgc
    24121 aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag acggtgaaag
    24181 acaatacgta attacgatgc cgggcgtggg ttgttggcgt ccggcgcttt accagatccc
    24241 ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct cccctaaccg cttcatcagg
    24301 tgaagcggat ctgacctgtc atcagaacga gagaattatg tttaaaccgg aactcctttc
    24361 cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag atgctgttta
    24421 tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac gaattcaacc acgaaaatct
    24481 tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg tggtcaacat
    24541 tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg tggtggaaat
    24601 ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc gtgagcactt
    24661 ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc gtgaactggg cgacggtgaa
    24721 attctggcag caaatgggac tgacccgcgt gatcctctct cgcgagctgt cgctggaaga
    24781 gattgaagag atccataatc aggtgccgga tatggagatc gagatcttcg ttcacggcgc
    24841 actgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca agcgcgaccc
    24901 gaaccagggc acctgcacca acgcctgccg ctgggagtac aacgtccagg aagggaaaga
    24961 agatgacgtt ggcaacatcg tacacaagta cgagccgatt ccggtgcaaa atgttgagcc
    25021 gacgctgggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag cccagcgtcc
    25081 gggcgagtat atgaccgcgt ttgaagatga gcacggcact tacatcatga actcgaaaga
    25141 tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg ggcgtgcatt cgctgaaaat
    25201 cgaaggtcgt accaaatctt tctactattg cgcacgcacc gcacaggttt accgcaaagc
    25261 tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc ctgctggaaa ctctggaagg
    25321 tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catactcacg acgattatca
    25381 gaactacgaa tacggttatt cagtttctga ccgccagcag tttgttggtg agtttaccgg
    25441 tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat aaattctccg ttggcgacag
    25501 tcttgagctg atgacgccgc aaggcaacat taactttacc cttgagcaca tggaaaacgc
    25561 caaaggcgaa gctatgccgg tcgcaccagg cgatggttat actgtgtggc tcccggtccc
    25621 gcaggatctt gagctaaatt acgcgctgct aatgcgtaat ttctccgggg aaaccacgcg
    25681 taacccccac ggtaagtgat taatttcgat tatttttccc ggatggaaaa ttcttagaaa
    25741 ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa aacataatcc
    25801 ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc cttacacggg
    25861 cttatttttt gtacataagc tgtaaagaag gattgatccc tgataaccgt cttccattga
    25921 ccacatcgat agaatccccc ttcatagcac aatgcctttc acgtaacggt atcgtgctcg
    25981 cccaggttcc ggctacgcac agccagaacg cgcatgtttg acgcttacaa aaaaatattc
    26041 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
    26101 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
    26161 tactatattt tctgcaccag atattctgcc cctggctaaa gaagcttcgg cttcggtgta
    26221 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
    26281 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
    26341 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
    26401 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
    26461 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
    26521 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
    26581 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
    26641 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
    26701 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
    26761 atcatggcag aatttcccgc cagcttactg attcttaatg gtaaaagtac tgacaatcta
    26821 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
    26881 acctgggaga aaggtgatgc cgcacgattt gtagaggagg cccggaagtt gggcgtcgca
    26941 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
    27001 tgtgaggggg atgacatacc cgcgctggga attttgccat taggaaccgc caatgatttt
    27061 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
    27121 aatgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
    27181 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
    27241 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
    27301 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
    27361 aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga acgcgttaat taacgatggc
    27421 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
    27481 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
    27541 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagtgggca aaacttccat
    27601 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtcc attattgcgt
    27661 taatcaaact attctctctt gaaccaggga gaatccgtaa tgccggatgc ggtgtaaaca
    27721 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
    27781 tgccaattta tccacctctg acacagcatc atcggtaaca ataatgtcaa ttttttcgat
    27841 tggcagtacc tgattaaaac cgcgacgatt aaatttcgat gaatcgagaa cggcaatcac
    27901 tttatttgcg gcggtgacca tcgccccgct aatcgaataa ccttcattaa aggtcgtaat
    27961 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
    28021 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
    28081 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
    28141 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtggtact
    28201 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
    28261 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
    28321 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
    28381 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
    28441 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
    28501 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
    28561 gggaccattg gtgaatgatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
    28621 gtttcatgga atgagcaaca ctttgccctg catggcatta cgagcgatgt cacgcaccgc
    28681 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
    28741 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
    28801 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
    28861 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
    28921 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
    28981 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
    29041 ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct gagctaatgt cgatcgccgt
    29101 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
    29161 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
    29221 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
    29281 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
    29341 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggac aagtaaaaca
    29401 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa cacc
//
LOCUS       DAJTRN010000046        29445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRN010000046 DAJTRN010000000
VERSION     DAJTRN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..431)
                     /locus_tag="OO328_002080"
                     /pseudo
     CDS             complement(1..431)
                     /locus_tag="OO328_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(406..1206)
                     /gene="otsB"
                     /locus_tag="OO328_002081"
     CDS             complement(406..1206)
                     /gene="otsB"
                     /locus_tag="OO328_002081"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5930649.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1373..2359)
                     /gene="araH"
                     /locus_tag="OO328_002082"
     CDS             complement(1373..2359)
                     /gene="araH"
                     /locus_tag="OO328_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5930650.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2386..3888)
                     /gene="araG"
                     /locus_tag="OO328_002083"
     CDS             complement(2386..3888)
                     /gene="araG"
                     /locus_tag="OO328_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5930651.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVS"
     gene            complement(3958..4947)
                     /gene="araF"
                     /locus_tag="OO328_002084"
                     /pseudo
     CDS             complement(3958..4947)
                     /gene="araF"
                     /locus_tag="OO328_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5744..6247
                     /locus_tag="OO328_002085"
     CDS             5744..6247
                     /locus_tag="OO328_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5930652.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6327..6578)
                     /locus_tag="OO328_002086"
     CDS             complement(6327..6578)
                     /locus_tag="OO328_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5930653.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6693..6779)
                     /gene="azuC"
                     /locus_tag="OO328_002087"
     CDS             complement(6693..6779)
                     /gene="azuC"
                     /locus_tag="OO328_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5930654.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7042..7365
                     /locus_tag="OO328_002088"
     CDS             7042..7365
                     /locus_tag="OO328_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5930655.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7536..8033
                     /gene="ftnA"
                     /locus_tag="OO328_002089"
     CDS             7536..8033
                     /gene="ftnA"
                     /locus_tag="OO328_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5930656.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8070..8309)
                     /locus_tag="OO328_002090"
     CDS             complement(8070..8309)
                     /locus_tag="OO328_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5930657.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8499..9710
                     /gene="tyrP"
                     /locus_tag="OO328_002091"
     CDS             8499..9710
                     /gene="tyrP"
                     /locus_tag="OO328_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5930658.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9772..10437)
                     /gene="yecA"
                     /locus_tag="OO328_002092"
     CDS             complement(9772..10437)
                     /gene="yecA"
                     /locus_tag="OO328_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5930659.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10633..10719)
                     /locus_tag="OO328_002093"
     tRNA            complement(10633..10719)
                     /locus_tag="OO328_002093"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10683..10685),aa:Leu,seq:taa)
     gene            complement(10732..10805)
                     /locus_tag="OO328_002094"
     tRNA            complement(10732..10805)
                     /locus_tag="OO328_002094"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10771..10773),aa:Cys,seq:gca)
     gene            complement(10860..10935)
                     /locus_tag="OO328_002095"
     tRNA            complement(10860..10935)
                     /locus_tag="OO328_002095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10900..10902),aa:Gly,seq:gcc)
     gene            complement(11087..11635)
                     /gene="pgsA"
                     /locus_tag="OO328_002096"
     CDS             complement(11087..11635)
                     /gene="pgsA"
                     /locus_tag="OO328_002096"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5930660.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11692..13524)
                     /gene="uvrC"
                     /locus_tag="OO328_002097"
     CDS             complement(11692..13524)
                     /gene="uvrC"
                     /locus_tag="OO328_002097"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5930661.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13521..14177)
                     /gene="uvrY"
                     /locus_tag="OO328_002098"
     CDS             complement(13521..14177)
                     /gene="uvrY"
                     /locus_tag="OO328_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5930662.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14473..14649
                     /locus_tag="OO328_002099"
     CDS             14473..14649
                     /locus_tag="OO328_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930663.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14636..14860
                     /locus_tag="OO328_002100"
     CDS             14636..14860
                     /locus_tag="OO328_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5930664.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14893..15513)
                     /gene="sdiA"
                     /locus_tag="OO328_002101"
                     /pseudo
     CDS             complement(14893..15513)
                     /gene="sdiA"
                     /locus_tag="OO328_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15743..16495)
                     /gene="yecC"
                     /locus_tag="OO328_002102"
     CDS             complement(15743..16495)
                     /gene="yecC"
                     /locus_tag="OO328_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5930665.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16492..17160)
                     /gene="tcyL"
                     /locus_tag="OO328_002103"
     CDS             complement(16492..17160)
                     /gene="tcyL"
                     /locus_tag="OO328_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5930666.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17175..18161)
                     /gene="dcyD"
                     /locus_tag="OO328_002104"
     CDS             complement(17175..18161)
                     /gene="dcyD"
                     /locus_tag="OO328_002104"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5930667.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18266..19066)
                     /gene="tcyJ"
                     /locus_tag="OO328_002105"
     CDS             complement(18266..19066)
                     /gene="tcyJ"
                     /locus_tag="OO328_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5930668.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19154..19705)
                     /gene="fliZ"
                     /locus_tag="OO328_002106"
     CDS             complement(19154..19705)
                     /gene="fliZ"
                     /locus_tag="OO328_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5930669.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19751..20470)
                     /gene="fliA"
                     /locus_tag="OO328_002107"
                     /pseudo
     CDS             complement(19751..20470)
                     /gene="fliA"
                     /locus_tag="OO328_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20635..22287)
                     /gene="fliC"
                     /locus_tag="OO328_002108"
     CDS             complement(20635..22287)
                     /gene="fliC"
                     /locus_tag="OO328_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5930670.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22553..23959
                     /gene="fliD"
                     /locus_tag="OO328_002109"
     CDS             22553..23959
                     /gene="fliD"
                     /locus_tag="OO328_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5930671.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23984..24394
                     /gene="fliS"
                     /locus_tag="OO328_002110"
     CDS             23984..24394
                     /gene="fliS"
                     /locus_tag="OO328_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5930672.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24394..24759
                     /gene="fliT"
                     /locus_tag="OO328_002111"
     CDS             24394..24759
                     /gene="fliT"
                     /locus_tag="OO328_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5930673.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24837..26324
                     /gene="amyA"
                     /locus_tag="OO328_002112"
     CDS             24837..26324
                     /gene="amyA"
                     /locus_tag="OO328_002112"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5930674.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26358..26771)
                     /locus_tag="OO328_002113"
     CDS             complement(26358..26771)
                     /locus_tag="OO328_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5930675.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26958..28163
                     /gene="yedE"
                     /locus_tag="OO328_002114"
     CDS             26958..28163
                     /gene="yedE"
                     /locus_tag="OO328_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5930676.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28160..28393
                     /gene="yedF"
                     /locus_tag="OO328_002115"
     CDS             28160..28393
                     /gene="yedF"
                     /locus_tag="OO328_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5930677.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28500..29170
                     /locus_tag="OO328_002116"
                     /pseudo
     CDS             28500..29170
                     /locus_tag="OO328_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29210..>29445)
                     /locus_tag="OO328_002117"
     CDS             complement(29210..>29445)
                     /locus_tag="OO328_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5930678.1"
                     /translation="PPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARN
                     ILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7329 a         7601 c         7010 g         7505 t
ORIGIN      
        1 attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat cgtggatcca
       61 gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg catttacgcg
      121 tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat aatgaaaagc
      181 gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct gttcgctgag
      241 gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt ccctgtttca
      301 ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat gccaacggca
      361 aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga tactacgact
      421 aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg cggcggttat
      481 catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg cctgagttgc
      541 acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga agccagattc
      601 atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag cttcctgcat
      661 aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga caacacactt
      721 tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta atgtcattaa
      781 tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc ctttcgcctc
      841 cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa tatcacgcgc
      901 aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc gctccgcccc
      961 atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct ccaccattga
     1021 gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct gtagtccttg
     1081 cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg ccagcgttcc
     1141 atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg ttaacggttc
     1201 tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc gctttacaga
     1261 catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca gccaaccgtt
     1321 gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag catcagacag
     1381 tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg attaagccgc
     1441 gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt tccacggtgc
     1501 ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct ttcagagaaa
     1561 cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac gtcattggct
     1621 gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc aggccagaga
     1681 gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc gcagcctctt
     1741 cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc agcaaaccaa
     1801 agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac cagttagcgt
     1861 aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct gaaatgatgt
     1921 acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca tttattttca
     1981 gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac aaacccgctg
     2041 ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg acacccgcac
     2101 aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc
     2161 cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata ttaatgaagg
     2221 tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac accaccagca
     2281 tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg ccagaccccg
     2341 atgtagaaac agaagacatc atactctcct tactcaggca actgcctagc tgactttagg
     2401 catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt caccggcgat
     2461 ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag gtaagtcgct
     2521 ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt tatatatttc
     2581 gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc
     2641 ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga gattcatgat
     2701 cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat cggcattgtt
     2761 ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc tggcactgat
     2821 gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc gagcttccgg
     2881 gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga tcggctgttg
     2941 gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt tcattaattc
     3001 gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc gaaccgccag
     3061 acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc gctcctcgcc
     3121 ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca tcgcctgcac
     3181 cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac gtccatcttt
     3241 aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag aaacgtataa
     3301 gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc cgatttcgcg
     3361 ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac gcgccagcgc
     3421 tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg gcgtgtccgg
     3481 gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca gcaatgagcg
     3541 attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg cgacggtcat
     3601 ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa gtgctgcggt
     3661 cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg ttggcgcata
     3721 gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac ccatcaacgc
     3781 atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa cgccgggaaa
     3841 cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa cgtctccgtg
     3901 aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc cggcaaatta
     3961 cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg tgatcagtac
     4021 cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc agttgtaaag
     4081 catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac cgtagaagcc
     4141 ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac caataccgat
     4201 gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa gtacggtgct
     4261 gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca gcattgagtt
     4321 ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat aaatttgttt
     4381 ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt
     4441 atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc agccacgttt
     4501 ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg ccgccatcat
     4561 caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt catccactgc
     4621 aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc
     4681 cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa
     4741 tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat ccttcccggc
     4801 tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct tcaccagaaa
     4861 accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca gaccaatggc
     4921 tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag
     4981 ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc ttttatctaa
     5041 aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat aggagagcaa
     5101 tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa gacataagga
     5161 aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata agattagcct
     5221 ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc agtcaatagg
     5281 ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca aagatgtgga
     5341 aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta acagaacacg
     5401 tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc acttcatcat
     5461 gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat taaaattcat
     5521 tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat gatttaagca
     5581 ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa ttgactccac
     5641 gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat ggtgttttga
     5701 tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa ccgctggaat
     5761 gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc tctaccttca
     5821 cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc ttcgcgccca
     5881 ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc
     5941 tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact
     6001 gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc agttagccga
     6061 tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga
     6121 acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact
     6181 tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt cacaccagct
     6241 gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag aaaccgctgc
     6301 ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt aaacttgagt
     6361 tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc ggcagcggga
     6421 tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac ttcaacgggc
     6481 gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc taccacatca
     6541 gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg atagcatttt
     6601 gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac cgtcatcagg
     6661 acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc tggcggtacg
     6721 tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg cagtttcatc
     6781 gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata atatgacaac
     6841 catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt aatgttataa
     6901 aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca agaaatagcg
     6961 cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc
     7021 tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg
     7081 gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt
     7141 gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg ttatgtcagt
     7201 cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat cccctttggt
     7261 cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac ggaattcact
     7321 ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt ttaatcagcg
     7381 aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat tcgtattttt
     7441 aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt atagtgacgc
     7501 cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa
     7561 aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg
     7621 cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg
     7681 aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc aatttaccgc
     7741 gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa ttattccagg
     7801 aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct catgctgcaa
     7861 tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct gagcagcatg
     7921 aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagcggcg
     7981 aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc
     8041 cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact ctgatcggtc
     8101 gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc taccagctct
     8161 cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct
     8221 ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat catcatattt
     8281 aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg
     8341 attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt gtacatttat
     8401 atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc
     8461 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     8521 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     8581 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     8641 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg
     8701 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     8761 tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa ttgttggcct
     8821 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     8881 cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt
     8941 ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata
     9001 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg tctgcaatcc
     9061 cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg
     9121 atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc cccctggtgg
     9181 catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc tttatgggat
     9241 tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtggcct
     9301 ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     9361 tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt tcaaataccg
     9421 ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     9481 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     9541 cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     9601 accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     9661 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     9721 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct cttaatgcag
     9781 gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac gccccggttt
     9841 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtatgc
     9901 atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     9961 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
    10021 tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
    10081 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
    10141 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
    10201 agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca catagtcagc
    10261 cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag aactcaacac
    10321 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
    10381 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
    10441 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
    10501 tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg acaagttgca
    10561 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt gattttattc
    10621 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
    10681 ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc
    10741 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
    10801 gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact
    10861 ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt gctctaccaa
    10921 ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg
    10981 caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat
    11041 atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca
    11101 aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca
    11161 caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac agcagccagg
    11221 ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg gccacgctac
    11281 tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga
    11341 tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt accagcacca
    11401 tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca aaccgggtac
    11461 tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga
    11521 aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga
    11581 atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga
    11641 cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt
    11701 caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc
    11761 ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg
    11821 acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg
    11881 ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac
    11941 atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct caaagaacag
    12001 cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt
    12061 tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt
    12121 gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg
    12181 ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt
    12241 atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac
    12301 ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa
    12361 tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt
    12421 gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc
    12481 gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag
    12541 cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg
    12601 gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc
    12661 gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa
    12721 tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc
    12781 ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat
    12841 acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc taatgagttg
    12901 cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc
    12961 gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctggta
    13021 ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa
    13081 aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa
    13141 atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc
    13201 actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg
    13261 atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt
    13321 tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct
    13381 gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt
    13441 accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc
    13501 ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg
    13561 cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga
    13621 tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc
    13681 ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat
    13741 gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatatcagaa
    13801 gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc
    13861 gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg taaagggttt
    13921 tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc gattttacgc
    13981 gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca
    14041 tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac gacttttata
    14101 cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca
    14161 agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt
    14221 cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga
    14281 tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg
    14341 gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt
    14401 cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg
    14461 caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagcact
    14521 gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc
    14581 gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa aataaatgag
    14641 tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct
    14701 gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat
    14761 gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc
    14821 caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgagagcg
    14881 tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
    14941 cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
    15001 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
    15061 ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa
    15121 gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
    15181 gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc acattaaatg
    15241 gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat tttttgcctg
    15301 ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
    15361 tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
    15421 ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    15481 gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    15541 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    15601 tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta
    15661 tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat
    15721 cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac tggcgggtgc
    15781 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct
    15841 ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa
    15901 tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact tcgcccacca
    15961 gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    16021 gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt ggatagctgg
    16081 tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt
    16141 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    16201 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    16261 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    16321 ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt ttgccggaac
    16381 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    16441 gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca ctcattttgg
    16501 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt
    16561 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    16621 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    16681 cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    16741 ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggcagcggc
    16801 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc
    16861 ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa
    16921 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat
    16981 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    17041 gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt gcagggtata
    17101 cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac tttcttgcat
    17161 gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc gccgccggta
    17221 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    17281 cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag acgcgccagc
    17341 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    17401 tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    17461 aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt
    17521 tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg
    17581 cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    17641 agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac cggaatgaca
    17701 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg ggcattggga
    17761 tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa caaacgattg
    17821 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag
    17881 tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga ctggatcgcg
    17941 ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag aaattccagc
    18001 ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt gatgaaaatt
    18061 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata
    18121 aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgtgtgt
    18181 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa
    18241 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    18301 aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc accgctttca
    18361 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    18421 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacgatcaa
    18481 cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg tcatcatcat
    18541 aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    18601 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    18661 cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt
    18721 tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca
    18781 gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    18841 ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    18901 aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc tctttgactt
    18961 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgg
    19021 ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg
    19081 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    19141 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    19201 cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    19261 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat
    19321 cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga acgacatatt
    19381 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta aagccgataa
    19441 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    19501 cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa
    19561 tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac
    19621 ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    19681 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    19741 tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc tggctgtgta
    19801 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    19861 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt
    19921 ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    19981 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca tcgtaggaga
    20041 agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata tcgatcccta
    20101 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    20161 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    20221 gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta aatgccgttc
    20281 cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc gcctgtagca
    20341 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca
    20401 ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga
    20461 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    20521 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa
    20581 aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag acgattaacc
    20641 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    20701 ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    20761 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    20821 ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga tggagctgat
    20881 ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt cagcatctgt
    20941 cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag tcactgaacc
    21001 atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt tgttatcaac
    21061 ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc tataacctgc
    21121 agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag ctgcctgatc
    21181 aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat taccggtaaa
    21241 ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt
    21301 ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc gcccagttgt
    21361 atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc tgatctgaac
    21421 atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa ctcccggggt
    21481 caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt atgacttact
    21541 tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt tattaatggt
    21601 tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt tagcagcatc
    21661 gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag ctaccaagtc
    21721 agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa acccattcag
    21781 ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc cgtcattcgc
    21841 accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt
    21901 ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct gaatggagtc
    21961 cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac ggatacgctg
    22021 taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc
    22081 gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat
    22141 cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag acagacgctc
    22201 gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga tcagcgagag
    22261 gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag tcgttgatta
    22321 cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa
    22381 acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga aatactcttt
    22441 tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt gcgcaattca
    22501 ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc gcatggcaag
    22561 tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg atagcctcac
    22621 cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt ttaccgctaa
    22681 acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg ccaatactgc
    22741 attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca ccgcgttcag
    22801 tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca cccatctggc
    22861 gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg cgatcgccac
    22921 cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta ccattgatat
    22981 cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg caaaagcggg
    23041 cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag tcacatcaaa
    23101 tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg cgctacaaag
    23161 ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg tcgccgccca
    23221 gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca ctatcagcga
    23281 cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc agacgctaac
    23341 catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga atgcctataa
    23401 ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg cgggagctga
    23461 tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga cgattcagac
    23521 gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa cgctggcgca
    23581 gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg acaaactcac
    23641 cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg atggtaaaaa
    23701 aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga caacgggcat
    23761 tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta aagactacaa
    23821 cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat ttacccaact
    23881 ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc agttcgaaaa
    23941 caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg cggcaaaagg
    24001 cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca gccaacagca
    24061 gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc gactgtttat
    24121 gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca acatcattga
    24181 gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc aaaacttaat
    24241 tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca acgatgtctc
    24301 agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga aagagtcttt
    24361 actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca tttgtatttc
    24421 gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac ggaagaacaa
    24481 tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga gatcgcacac
    24541 ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc gatgctgcac
    24601 ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat ggatgaactg
    24661 gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta tggcgatcag
    24721 ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag ccgatggctc
    24781 gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt cgaagaatgc
    24841 gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt aagctctggc
    24901 ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg gtctggttgc
    24961 cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc tatgatttat
    25021 ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt gataaagcac
    25081 aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg ctggacgtgg
    25141 tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg
    25201 ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg acgcgttaca
    25261 ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa tgttttagcg
    25321 gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt aacgactata
    25381 ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat tatctgatgg
    25441 gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac tgggcgcgct
    25501 gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa catattccgg
    25561 catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag ccgctgttta
    25621 ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt gatcaggtgg
    25681 aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca
    25741 tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa gccgatcctt
    25801 ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc ctcgaagcac
    25861 cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg
    25921 ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc ggtggtgacg
    25981 ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta attctcgccc
    26041 gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac tgcattgcct
    26101 ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg
    26161 atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg cgtgattttc
    26221 tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc ttcttttgca
    26281 acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat ccccggcggc
    26341 aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg
    26401 gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta ctcaatggtg
    26461 ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt gagatcatca
    26521 gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg gcaatccaag
    26581 gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac cagcgcacgt
    26641 tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt tttcacgaca
    26701 ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg
    26761 agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt aaagctctga
    26821 tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt ttgtgaatta
    26881 ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca aaccttctga
    26941 gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg attaaattct
    27001 gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat tttggcatta
    27061 ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag ctcctgcagc
    27121 tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg gaaggatcgc
    27181 cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc tttgctgctg
    27241 cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc attatgcagg
    27301 ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc tgtaacctgg
    27361 cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt gccatcgcca
    27421 ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc cgtattcccg
    27481 tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat caggcgcggc
    27541 gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg ctgctcacgg
    27601 cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt ggtttactga
    27661 ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc accggacgta
    27721 cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg atcttcagtt
    27781 acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg gtaattggtg
    27841 gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc tggatgtacc
    27901 gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg atcggctcaa
    27961 cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac tgggacaaaa
    28021 tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat ttgctgctgt
    28081 ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt gaggcaccgc
    28141 agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg atatggtggg
    28201 tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat taaaaaaagg
    28261 tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc cactggatgc
    28321 gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca ttcgttattt
    28381 aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg ttacctctga
    28441 cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag gaggttgcta
    28501 tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt ctcgcggaag
    28561 atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat gtcgcgccgg
    28621 gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct
    28681 ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg
    28741 cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg
    28801 atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta tctatcgcgc
    28861 tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc gtggtgacga
    28921 agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag atattcatga
    28981 ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc aagacattgg
    29041 tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc agttcacctg
    29101 gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt taattcaacc
    29161 tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt caggctgtcg
    29221 cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc atctctccac
    29281 aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca tcggcgttcg
    29341 ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt tctttcgctg
    29401 tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaa
//
LOCUS       DAJTRN010000047        29372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRN010000047 DAJTRN010000000
VERSION     DAJTRN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..579
                     /locus_tag="OO328_002118"
     CDS             <1..579
                     /locus_tag="OO328_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5930679.1"
                     /translation="IAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAE
                     RVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNG
                     TNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKL
                     EHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            601..2010
                     /locus_tag="OO328_002119"
     CDS             601..2010
                     /locus_tag="OO328_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5930680.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1988..2767
                     /locus_tag="OO328_002120"
     CDS             1988..2767
                     /locus_tag="OO328_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5930681.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2764..3624
                     /locus_tag="OO328_002121"
     CDS             2764..3624
                     /locus_tag="OO328_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5930682.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3772..4347)
                     /locus_tag="OO328_002122"
                     /pseudo
     CDS             complement(3772..4347)
                     /locus_tag="OO328_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4364..4624)
                     /locus_tag="OO328_002123"
     CDS             complement(4364..4624)
                     /locus_tag="OO328_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5930683.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4615..5886)
                     /locus_tag="OO328_002124"
     CDS             complement(4615..5886)
                     /locus_tag="OO328_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5930684.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5964..6326)
                     /gene="queD"
                     /locus_tag="OO328_002125"
     CDS             complement(5964..6326)
                     /gene="queD"
                     /locus_tag="OO328_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5930685.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6645..8444
                     /gene="cysJ"
                     /locus_tag="OO328_002126"
     CDS             6645..8444
                     /gene="cysJ"
                     /locus_tag="OO328_002126"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5930686.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8444..10156
                     /gene="cysI"
                     /locus_tag="OO328_002127"
     CDS             8444..10156
                     /gene="cysI"
                     /locus_tag="OO328_002127"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5930687.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10230..10964
                     /gene="cysH"
                     /locus_tag="OO328_002128"
     CDS             10230..10964
                     /gene="cysH"
                     /locus_tag="OO328_002128"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5930688.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11228..11380
                     /locus_tag="OO328_002129"
     CDS             11228..11380
                     /locus_tag="OO328_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5930689.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11574..12665
                     /locus_tag="OO328_002130"
                     /pseudo
     CDS             11574..12665
                     /locus_tag="OO328_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12811..13848)
                     /gene="iap"
                     /locus_tag="OO328_002131"
     CDS             complement(12811..13848)
                     /gene="iap"
                     /locus_tag="OO328_002131"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5930690.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14100..15008
                     /gene="cysD"
                     /locus_tag="OO328_002132"
     CDS             14100..15008
                     /gene="cysD"
                     /locus_tag="OO328_002132"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5930691.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15010..16437
                     /gene="cysN"
                     /locus_tag="OO328_002133"
     CDS             15010..16437
                     /gene="cysN"
                     /locus_tag="OO328_002133"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5930692.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16437..17042
                     /gene="cysC"
                     /locus_tag="OO328_002134"
     CDS             16437..17042
                     /gene="cysC"
                     /locus_tag="OO328_002134"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5930693.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17092..17415
                     /locus_tag="OO328_002135"
     CDS             17092..17415
                     /locus_tag="OO328_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5930694.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17609..17920
                     /gene="ftsB"
                     /locus_tag="OO328_002136"
     CDS             17609..17920
                     /gene="ftsB"
                     /locus_tag="OO328_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5930695.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17939..18649
                     /gene="ispD"
                     /locus_tag="OO328_002137"
     CDS             17939..18649
                     /gene="ispD"
                     /locus_tag="OO328_002137"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5930696.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18649..19128
                     /gene="ispF"
                     /locus_tag="OO328_002138"
     CDS             18649..19128
                     /gene="ispF"
                     /locus_tag="OO328_002138"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5930697.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19125..20174
                     /gene="truD"
                     /locus_tag="OO328_002139"
     CDS             19125..20174
                     /gene="truD"
                     /locus_tag="OO328_002139"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5930698.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20155..20916
                     /gene="surE"
                     /locus_tag="OO328_002140"
     CDS             20155..20916
                     /gene="surE"
                     /locus_tag="OO328_002140"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5930699.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20910..21536
                     /gene="pcm"
                     /locus_tag="OO328_002141"
     CDS             20910..21536
                     /gene="pcm"
                     /locus_tag="OO328_002141"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5930700.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21676..22815
                     /gene="nlpD"
                     /locus_tag="OO328_002142"
     CDS             21676..22815
                     /gene="nlpD"
                     /locus_tag="OO328_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5930701.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22878..23870
                     /gene="rpoS"
                     /locus_tag="OO328_002143"
     CDS             22878..23870
                     /gene="rpoS"
                     /locus_tag="OO328_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5930702.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23990..24397)
                     /locus_tag="OO328_002144"
     CDS             complement(23990..24397)
                     /locus_tag="OO328_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5930703.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24544..25137
                     /locus_tag="OO328_002145"
     CDS             24544..25137
                     /locus_tag="OO328_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5930704.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25137..26084
                     /locus_tag="OO328_002146"
                     /pseudo
     CDS             25137..26084
                     /locus_tag="OO328_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26357..26920)
                     /locus_tag="OO328_002147"
     CDS             complement(26357..26920)
                     /locus_tag="OO328_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930705.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27085..27336
                     /locus_tag="OO328_002148"
     CDS             27085..27336
                     /locus_tag="OO328_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5930706.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27338..>29372
                     /locus_tag="OO328_002149"
     CDS             27338..>29372
                     /locus_tag="OO328_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5930707.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFER"
BASE COUNT         7162 a         7290 c         7965 g         6955 t
ORIGIN      
        1 attgcgaagg cgacgggcga agggattgcg gaaatcgttg cccgctaccc gcagtgccag
       61 cgcgtggaca gcaaactgat tgaaacctgg tttaacaacc tgaactgggg accggataaa
      121 gtggctgccg aacgtgtgca gatcctcaaa accggcaaca tgggctttac caccgaagtg
      181 tccggctgct ggagctgcat ccacgaaatc tacgaaagcg ttattaaccg tattcgtact
      241 gagttcccgc acgccgacga catcaccatg ctgggcggtc actcctctca tagctatcag
      301 aacggcacca acatgtactt cgtctatgac tacaacgttg ttgactgtaa gccggaagag
      361 gaaatcgaca agtaccacaa tccgctcaac aaaatcatct gcgaagaaac cattcgcctc
      421 ggcggttcga tggtgcacca ccatggcatt ggtaaacatc gcgttcactg gagcaagctg
      481 gaacacggca gcgcgtgggc gttgctggaa gggctgaaaa agcagttcga tcctaacggc
      541 attatgaata cgggtactat ctatccgatt gaaaaataat gtatcaggca gcgttccgcg
      601 atgacggggc gttgcctttt cggcttctca ggcgagaagc cgttcttatt accggacaat
      661 gaaggggtaa agatgaacac ttcaccggtg cgaatggatg atttaccgct taaccgtttt
      721 cactgtcgaa ttgctacgct cactttcggc gcacacctga ccgacggtta tgttctaggc
      781 gtcattggtt acgccattat tcaacttacg cccgccatgc aactgacgcc gtttatggcg
      841 ggaatgatcg gcggctcggc gctccttggt ttgtttcttg gcagcctggt tcttgggtgg
      901 atctccgacc atattggtcg gcaaaaaatc ttcaccttca gctttttgct gattacgctc
      961 gcttcgttct tgcaattttt tgccaccacg ccagagcatc ttattgggct gcgcattttg
     1021 atcggcattg gtctgggagg cgattactca gtaggtcaca ccttgctggc tgaattttcc
     1081 ccgcgccgcc atcgcggtat tttgctgggc gcattcagcg tggtgtggac cgtaggctat
     1141 gtgttggcaa gtattgccgg acatcacttt atttccgaaa acccggaggc ctggcgctgg
     1201 ttgctggcat cggcagctct gcccgcgttg ttgattacgt tattacgctg gggaacgcca
     1261 gaatcgccac gttggctact gtgccagggg cgttttgcag aagctcacgc tatcgtgcat
     1321 cgctattttg gtccccatgt tttactgggc gatgaagtgg taacggcgac ccataaacac
     1381 atcaaaacct tgttctcttc gcgttactgg cggcgcacgg cgtttaacag cgtcttcttt
     1441 gtctgcctcg taatcccatg gtttgtgatt tatacctggc tgccaactat cgcccagact
     1501 attggtctgg aagatgcgct gactgccagc ctgatgctta atgcgttgtt aattgtgggc
     1561 gcgctgctgg gattagttct gacgcacctg ctggcacatc gcaaattttt gctgggaagt
     1621 tttttgctgc tggcggcaac gctggtagta atggcctgtt tgccttccgg cagttcatta
     1681 acgctgctgc tttttgttct cttcagcacc accatttcgg cagtcagtaa tctggtgggc
     1741 attttgcctg cggaaagttt tcctactgac attcgctcgc tgggagtcgg ttttgccacc
     1801 gccatgagtc gacttggcgc ggcggtaagt actggcctgc tgccgtgggt gctggcgcag
     1861 tggggaatgc aagtcacctt attgctccta gcgacagtgt tgttggttgg ttttgttgtg
     1921 acctggctat gggcaccaga aactaaagcc ctcccgctgg tggcggcggg aaatgtagga
     1981 ggtgcgaatg aacattctgt tagcgtttaa agccgaaccg gatgccggaa tgctggcgga
     2041 aaaagagtgg caggcggcgg ctcagggtaa tagcggaccg gatgtttcgc tactgcgaag
     2101 tttactcggt gctgatgaac aggccgctgc cgcgctgctg ttagcgcaga gaaaaaacgg
     2161 tacgccgatg tctttaaccg cgttgagcat gggggatgaa cgggcgttgc actggctgcg
     2221 ctatctcatg gctcttgggt ttgaggaagc tgtattgctg gagacggcgg cagatctgcg
     2281 ctttgcgccg gaatttgtcg ctcgccatat tgccgaatgg cagcgtcaga atccgctgga
     2341 tctgataatt accggcagcc aaagcagcga agggcagaat gggcaaacgc catttttgct
     2401 ggcggaaatg ctgggctggc cctgcttcac ccaggtggag cgtttcactc tcgacgcgct
     2461 gtttatcacc ctcgaacagc gtactgaaca tgggttgcgc tgttgccggg ttcgcttgcc
     2521 tgcggtgatt gccgtgcgtc agtgcggtga agtggccttg ccagtaccgg gaatgcgcca
     2581 gcgaatggct gcggggaaag cagaaattat ccgtaaaact gttgctgccg aaatgccagc
     2641 gatgcagtgt ctgcaactgg caagagcaga gcaacgacgc ggagcgacgc tgattgatgg
     2701 gcaaacggta gcagaaaaag ctcaaaagct atggcaggac tatttacgcc aaaggatgca
     2761 gccatgaata tcgcaattgt gacaataaat caggagaatg ccgcaatagc gagctggctg
     2821 gcggcgcagg atttcagcgg ttgtacgctg gcgcactggc aaatagaacc tcagcccgta
     2881 gtggcggaac aggtgctgga tgcgatggtg gaacagtggc agcgaacgcc tgcggacgtt
     2941 gttctctttc cgccgggaac gtttggcgat gaactgtcga cccggctggc ctggcgttta
     3001 catggtgcaa gcatctgtca ggtgacttcg ttagatattc caaccgtaag catgcgcaaa
     3061 tcccactggg gaaatgcgtt aacggcgacg ctgcaaacag aaaaacgccc gttatgcctt
     3121 tccctggcgc gtcaggcggg agcagcgaaa aacgccacgt tgccctctgg aatgcagcaa
     3181 ctgaacattg tcccaggtgc gcttcccgac tggctggtta gcacagagga tctgaaaaat
     3241 gttagccgcg acccattagc tgaggccaga cgggtgctgg tggtgggaca gggcggagag
     3301 gcagataacc aggaaatcgt tatgctggca gaaaagctgg gagccgaagt gggctacagt
     3361 cgggcgcgag tgatgaacgg tggcgttgat gctgaaaaag tgattggtat ttccggacat
     3421 ctgctggcac ctgaggtgtg cattgttgtt ggcgcttccg gcgcagcagc gttaatggcg
     3481 ggcgtacgca atagcaagtt tatggtggcg attaatcatg acgccagcgc agcggtattt
     3541 tcacaggcag atattggcgt ggttgatgac tggaaagtgg tgctggaggc actggtgaca
     3601 aacattcacg cagaatacca ataacgtgct gccggatgat cctgcctacg gttctggact
     3661 ggggacctgt aggcctgata agacgcgtta agcgtcgcat caggcgctga atgtcggatg
     3721 tggcgtgcgg ccaacaaatc aaaacaaatt caattggtta cgattatccc gtcaaagcaa
     3781 ttttttcgct aacgtccaga ctccggtatt cgtggtggaa agcgccacga cacccgcgtt
     3841 aatcgcatta cgcgcatctt cttcatcgca taccagcccg ccagcaatca gcggttggcg
     3901 gattttttcc gtcacccagc ccagcacttt gggcatgcag cctggcagga tctcaataca
     3961 atccggattc gattgcgcaa cttacttatc aatgttgtga aacgaaatcg aatcaacaat
     4021 aaacaggtga tgaatacaga aaaaaacttc cgctcttgcc gcttttagca tcgatgcttt
     4081 ggtgctgata attccatccg cctcggtcac cagtttcaaa aactgaatca ccacttcttt
     4141 atttgacgcg ccttccagca aatcaacatg aataaaagca tatttcccgg cgttcttaat
     4201 ctttttaacg atattactga tggtgcagat attgccgtac aacacggaaa taaactggca
     4261 ttcagaatcg atggccagtt gcaggctggc attatcttta acggcagcaa tcaccggatt
     4321 ctggcggagc aggtgtaaga ggggcataaa ttatccttat tatttatcca aagcggtagg
     4381 tgatgccgaa tccggaaggc ggatagcgcc actgttgtag tgtggattcg tcgcacagca
     4441 aacggcaggt gccacactcc aggcagccgc gatagtcaat acgtaagtca ccttcctgtg
     4501 tgagagaaaa aagacctacc ggacaggcgt taatcaaccg ttgtaccatc tgacgctcaa
     4561 cggagtcagc cggaacaatg tgcggcgcat cagcaacgcg ccagagatta cgggctacag
     4621 acatcgcaga ctcctgataa catcgcccgc cagatgccac aggccatgac gacgtaaatg
     4681 atgccagaat aactggcgca gcggtggaac aggtttatca ccctgatccc ataaatcgcg
     4741 ggaaatatcc tgcattaacg caggccacgc ccgatacctg ccagggcgtt gcaaaagcgc
     4801 cggaacatgc tgataacgtt gtagaacatc ccatagcagg ctgcgctcta cgttgtgatg
     4861 ataaagcgca aacagatttt gcggctcgcg gtgctggcag gcgcttatta gcgtttgtgc
     4921 cgccgcctgt gcgccagtca gcgccatatc catgccgcgc acggaaattc cggtattgac
     4981 gcaactgcgc aacgcatcgc ccaccagcag ccagccgtta ccggcgtatt gcaccggcat
     5041 actgtgcaag ccaccttctg gcaccagatg cgcaccatac tccagtgatt ccgtgttttt
     5101 gataagcgtg cgcactgccg gatgcgcttt aaagcgagtc agcagctcgc ttgccggaac
     5161 acgactttgc gtaagggaag agagcgggca aacaatccct aacgagagcg tttgttgatt
     5221 agtataaaga aatgcgccgc cgggtaagtc atcacagatt ccgccgctga acagcaacgc
     5281 tgcgccttcg ttattctcca gatgaaaacg ttcttcaata gcggatgttt ccagcgacag
     5341 cacttctttg atccccaacg ccatcgcttc gccagcagga cgagtcacta acccgtgacg
     5401 ttcagccagg acgctgttgg caccttctgc cagcaccaca taacgggcgc ggagaatatc
     5461 gtcaccacaa ataacgccac agactctgcc gttttcttca tacagtgcat ccaccgtcgc
     5521 tccggggatg cattcgacac cttctttttc ggcttcggca accagccacg gatcgaaccg
     5581 tgcacgtaat acattccagg attcaccgcc gggctgtaag ctggaaaacg tcgttgcgcc
     5641 atccggcgtt aacagggaaa ggctttcgtg agtgatgcgt cgttcaagag gcgcggtcag
     5701 atgaaattgc gggaggagtt ccgcgagtgc atgggtatat aaccgcctgc cggaaagatt
     5761 tttgctgccg gggatttcag cgcgttcgag taacaaaacg gataaatccg ctcgcgcgca
     5821 gcgtaacgcg caagcggtgc ctgcaatacc ggcaccaata ataataatgt cgcagtcgtc
     5881 ttccatgtca tgccccagat atccagaaca caacacccta acatagcgtt actaaaggga
     5941 aattgaccgc cgacacactc ttatcattcg ccgcgataga tacaccctgc ggtgcaggtt
     6001 tcttttacca tcaccgcact taacagcggc acaacgggtt taacctgatc ccaaatccat
     6061 tttgctaaaa cttcgctggt tggattttcc agacctggaa tatcattgag ataatggtga
     6121 tcgaggcgct cataagttgg cttaaacgcc gctttcagtt cagcgaaatc gataatccag
     6181 cccgtatgcg gatcgacttc cccggtaatt tccagtcgca ccataaagga atgcccgtgc
     6241 aggcgaccac atttatgccc ttccgggaca tgtggtaagc ggtgagcggc ttcgaaggtg
     6301 aaatctttaa ataacgtggt ggacatcata atttctctac aggacgataa aaccgccgta
     6361 gagtaccgga aaggctgttt tttcgcatta tctaaaacga ctttgttgcg caaaatcgct
     6421 gatttatctt aatgattggc taaattcatt tgtttttcat taggttggtt aatctatttt
     6481 gttgttaaag actattgcta aaacaggtta gtcgatttgg ttattagtta tcgctatccc
     6541 gtctttaatc cacaccgttc accccgttaa ccttaccttc tcttctgttt tatgggcgct
     6601 gacagggcgc agaaacagct ttgcttactg gaacataacg acgcatgacg acacaggtcc
     6661 caccttccgc gttgcttccg ttgaacccgg agcaactggc acgccttcag gcggccacga
     6721 ccgatttaac gcctacccag cttgcctggg tttctggcta tttctggggc gtgctcaacc
     6781 agcagcctgt tgcgcttgca gtgacgccag cgcccgccgc agaaatgccg ggtataacga
     6841 ttatctctgc ttcgcaaacc ggcaatgcgc gccgggttgc tgaagcatta cgcgatgatt
     6901 tattagcagc aaaactgaac gttaagctgg tgaacgcggg cgactataaa ttcaaacaaa
     6961 tcgccagcga aaaactgctc atcgtagtga cgtcaacgca aggggaaggg gaaccgccgg
     7021 aagaagccgt cgcgctgcat aagttcctgt tctccaaaaa agcgccaaag ctggaaaaca
     7081 ccgcgtttgc cgtgtttagc ctcggcgata gctcttatga atttttctgc cagtccggga
     7141 aagattttga cagcaagctg gcggaactgg ggggtgaacg cctgctcgac cgtgtcgatt
     7201 ctgatgttga ataccaggct gctgccagcg agtggcgcgc ccgcgtggtt gatgcgctta
     7261 aatcgcgtgc gcctgtcgcg gcaccttcgc aatccgtcgc tactggcgcg gtaaatgaaa
     7321 tccacaccag cccgtacagc aaagacgcgc cgctggtggc tagcctctct gttaaccaga
     7381 aaattactgg gcgtaactct gaaaaagacg ttcgccatat cgaaattgac ttaggcgact
     7441 cgggcctgcg ttaccagccg ggtgacgcgc tgggcgtctg gtatcagaac gatccggcac
     7501 tggtgaaaga acttgtcgaa ctgctgtggc tgacaggcga tgaacctgtc actgtcgagg
     7561 gcaaaacgtt gcctctgaac gaagcgctac agtggcactt cgaactgacc gtcaacaccg
     7621 ccaacattgt tgagaattac gccacgctta cccgcagtga aacactgctg ccgctggtgg
     7681 gcgataaagc gaagttacag cattacgccg cgacgacgct gattgttgac atggtgcgtt
     7741 tctccccggc acagcttgat gccgaagcgc taattaatct gctgcgcccg ctgaccccgc
     7801 gtctctattc cattgcctcc tcgcaggcgg aagtcgagaa cgaagtacac gtcaccgttg
     7861 gtgtggtgcg ttacgacgtg gaaggccgcg cccgtgccgg tggtgcctcc agcttcctcg
     7921 ctgaccgcgt ggaagaagag ggcgaagtcc gcgtatttat cgaacataac gataacttcc
     7981 gcctgcccgc taacctggaa accccggtga ttatgattgg cccaggcacc ggtattgcgc
     8041 cgttccgcgc ctttatgcag caacgcgccg ctgacgaagc gccaggtaaa aactggctgt
     8101 tctttggcaa tccgcatttt acggaagact tcctgtatca ggtggagtgg cagcgctacg
     8161 tcaaagaggg tgtgctgacg cggatcgatc tcgcctggtc gcgcgaccaa aaagaaaaag
     8221 tttacgtaca agataaactg cgcgaacagg gcgcggagct gtggcgctgg atcaatgacg
     8281 gtgcccacat ttatgtctgc ggcgacgcta atcgcatggc gaaagacgtt gagcaggcac
     8341 ttctggaagt gattgccgaa tttggtggca tggacaccga agcggcggat gaatttttaa
     8401 gtgagctgcg cgtagagcgc cgttatcagc gagatgtcta ctaatgagcg aaaaacatcc
     8461 agggccttta gtggtcgaag gaaaactgac agacgccgag cgcatgaagc ttgaaagcaa
     8521 ctacctgcgc ggcaccattg cggaagattt aaacgacggt ctgaccggcg gctttaaggg
     8581 cgataacttc ctgctgatcc gcttccacgg catgtatcag caggatgacc gcgacatccg
     8641 cgccgaacgt gctgaacaga agctggagcc gcgccacgcg atgctgttgc gctgccgtct
     8701 gccgggtgga gttatcacca ccaaacagtg gcaggcgatc gataaatttg ccggtgaaaa
     8761 caccatctat ggcagcattc gcctgaccaa ccgccagacg tttcagttcc acggcattct
     8821 gaaaaagaac gtcaaaccgg tgcaccagat gctgcactcg gtcggtcttg atgcgctggc
     8881 gactgctaac gacatgaacc gtaacgtact ctgcacctcg aacccttacg agtcgcagct
     8941 gcacgcagaa gcgtacgagt gggcgaagaa gatttctgaa catctgctgc cgcgtacccg
     9001 cgcgtatgcg gagatctggc tcgatcagga aaaagtcgcc accaccgatg aagaaccgat
     9061 ccttggtcag acttacctgc cgcgtaagtt caaaaccacg gtagtgatcc cgccgcagaa
     9121 cgatattgat ctgcacgcca acgacatgaa cttcgtggcg atcgcggaaa acggcaagct
     9181 ggtgggcttc aacctgctgg tgggcggtgg gctttctatc gaacacggta acaagaaaac
     9241 ctacgcccgc acggcgagtg agtttggcta tctgccgcta gagcatacgc tagcggtggc
     9301 ggaagccgtc gtgacaactc agcgtgactg gggtaaccga accgatcgta aaaatgccaa
     9361 aaccaaatac acactggagc gcgtgggggt tgagacgttt aaagcggaag tggagcgtcg
     9421 cgcggggatc aaatttgaac cgatccgccc gtatgaattt acaggacgcg gcgatcgtat
     9481 tggctgggtt aagggcattg atgataactg gcacctgacg ctgtttatcg aaaatggtcg
     9541 catccttgat tatccggggc gtccgctgaa aactggcctg ctggagatcg cgaagatcca
     9601 caaaggtgat ttccgcatta cggcgaacca gaatctgatc atcgccggtg tgccggaaag
     9661 cgagaaagcg aagatcgaga aaattgccaa agagagcggg ttaatgaatg ccgtcacgcc
     9721 gcagcgtgaa aactcgatgg cctgcgtgtc attcccgact tgcccgctgg cgatggcgga
     9781 agcggagcgt ttcctgccgt cttttatcga caacatcgat aatttaatgg tgaaacatgg
     9841 tgtcagcgat gagcatatcg tgatgcgtgt aacaggctgc ccgaacggtt gtggtcgcgc
     9901 gatgctggcg gaagtgggcc tggtgggtaa agcaccgggt cgctacaacc tgcatcttgg
     9961 cggcaaccgc attgggacac gtatcccacg gatgtataaa gaaaacatca ccgagccgga
    10021 aatcctggcg tcgcttgatg aactgatagg gcgctgggcg aaagagcgcg aagcgggtga
    10081 aggcttcggc gactttacgg tgcgtgcggg catcattcgc ccggtgctcg atccggcgcg
    10141 cgatttgtgg gattaaccat cagcccggtc ttgtaggcct gataagacgc gtgagcgtcg
    10201 catcaggcaa ggcaaacagt gaggaatcta tgtccaaact cgatctaaac gccctgaacg
    10261 aactgccgaa ggtagatcgc attctggcgc tggcagaaac taacgccgaa ctggaaaaac
    10321 tggacgctga aggccgcgtt gcctgggcgc tggataatct gcccggtgaa tatgtacttt
    10381 cttccagctt tggcattcag gcggcggtga gcctgcatct ggtgaatcag attcacccgg
    10441 atattccggt gatcctcacc gacaccggct atctgttccc ggaaacctac cgctttattg
    10501 acgagttaac ggacaaactc aagctcaacc tgaaagtgta ccgcgctacc gaaagcgccg
    10561 cctggcagga agcacgctac ggcaaactgt gggaacaggg cgttgaaggc attgaaaagt
    10621 acaatgacat caacaaagtc gaaccgatga accgggcgct gaaagaactg aatgcgcaaa
    10681 cgtggtttgc tggcctgcgg cgtgaacaat ccggcagccg tgccaattta ccggtgctgg
    10741 caattcagcg tggcgtattt aaagtgctgc cgattatcga ctgggataac cgaactattt
    10801 atcagtacct gcaaaaacat ggcctgaaat atcacccatt atgggacgaa ggatatttat
    10861 cggtgggcga tacccataca acccgtaaat gggaacccgg catggcggaa gaagaaacgc
    10921 gtttctttgg cttaaaaagg gaatgtggat tacacgaagg gtaaatattg ccggataaat
    10981 taaacgcctc tgtcagaaat gatgggggcg tttttatttt gcgagcagca ttccggcatt
    11041 taaaggtgaa catcgcaggg aaaattaaca ggtatagaat ggcgttgggt gcttgaggct
    11101 gtctggcgtt aagcattcgc gaggttccag atggacaaaa gccccaggcg atatttctat
    11161 caacctgagg ctcgcgttcg aacgtagcaa ttcggatgtt agtctcttct ttgcagggag
    11221 gcaagacatg ctgacaaaat atgcccttgt ggcaatcatc gtactgtgtt gtacagtact
    11281 gggattcacg ttgatggtag gtgactcgtt atgtgagttg agtatcagag aacgtggtat
    11341 ggagtttaag gcagttctcg cttacgaatc gaagaagtag ccaccacgcg ggggtaaaac
    11401 cccgcgtact gaattgtgat ggtttggtct cctgcaccct ttttttaaat ttatattttt
    11461 tatgtgaata tatagtctct atttattatt atgaatttca aatgaaaatc attaatttat
    11521 tcataatgta aatgttttat tggctgatat atatcataga tagtttttaa aatatgcgta
    11581 aatatccttt aagtttactg aaggataaaa atattgtgac tttctttgat ttctggggaa
    11641 aaaaccgacg tggcgagaaa gagggtatcg acgactatca ccttctttgc tggcatttgc
    11701 tggatgtggc cgcaatgggc tatttaatgg ttaaaagaaa ttgcttcggg ctggctgatt
    11761 attttcgtca attagggatt tctgacaagg aacaggcagc tcgatttttc gcctggttgc
    11821 tgtgctggca cgatattgga aaatttgccc gctcttttca acaactttac ctgccccctg
    11881 aattcaagat tcaggaaggt gccagaaaaa attacgaaaa aatctctcat tcaacgctgg
    11941 gctactggtt gtggaatcat tatttaagtg aatgtcagga gttacttcct tcatcttcgc
    12001 tctctccgcg caaacttagg cgagtgatag aaatgtggat gcccgtgacg acagggcatc
    12061 atggtcggcc ccctgatcgt atggatgaat tggataattt tctgcctgaa gacaaagctg
    12121 cagcacgaga ttttctcctt gaaatcaagc cactgtttcc gcgaatagag atccccacat
    12181 tctgggatga tgacgagggc gttgaactta taaaacaact ttcctggtat atctctgcaa
    12241 ccgtcgtact tgcagactgg acagggtcgt caacgcgatt ttttccacgc gtcgcacagg
    12301 caatggatat tgaagattac tggcaaaagg ctttagttca ggctcaaaac gcattaaccg
    12361 tctttccgcc acaagcagaa accgcgcctt tcaccggaat taatacgctg tttcctttta
    12421 ttgagaatac gacaccgtta cagcaaaagg tgctggatct ggatatcaat cagccaggac
    12481 cacagttgtt tattctggaa gatgtgaccg gcgcaggtaa aacagaagcg gcgcttatcc
    12541 tggcgcacag gttgatggct gcgggtaacg ctcaggggct gttttttggc ctgccaacaa
    12601 tggcaacggc caatgccatg tacgatcgac tggtcaaaac ctggcttgct ttctattcgc
    12661 cagaggtgtt ccccgctcca gcggagataa accggcgcaa tacggtgcgg ttttcccgga
    12721 agtaaaccgg acgcactgga tgcgatgatg ggtatcactt agaattcccc gcccctgcgg
    12781 tagaactccc agctcccatt ttcaaaccca tcaagacgcc ttcgccagct ccttcaccaa
    12841 cggtagcatt atccgcataa cgtcacggca gcgacgttct attcttccag gaagtgcctt
    12901 atcaatatgt tgttgattat ccagtcttac gtcatgccag ctatttcccg ccgggaaggc
    12961 aggtgttttt gcgcgttgct gataaccatc cttatttcca agattccagt tagtcgcttc
    13021 caccgaaagt acagcaatgc ccgctttgtc gaatatttct gcgtcattac aacccccagt
    13081 gcctttcgga taatttttat tcaaacccgg attggtcgtt gcggctattc cgtgactgcg
    13141 tgcaattgcc agcgccctgt cgcgcgttaa tttccttact gcttcagggg tttttacacc
    13201 gctgttgaaa tacaatttat cgccaacaat taagttatcg agattaatca ccagcagcgt
    13261 atttttcttt tcggtgtcac tcatccgctt gagtaaattc tcagcgccta attttccttc
    13321 ctcttcgccg ctggtcgcca caaatcgaat accatactcg gtaggcgtat ttttcaggcg
    13381 ttctgccagt tccagcatga cacctaaacc tgcggcgtta tcatctattc cttgtaacgt
    13441 cagtccgccg agattggcat ctgcgtcagc atcgctcagc ggggcgtaag tatccagatg
    13501 cgccataatg atgatctgct gcggcgcttt gccttcatga gcggcaatca ccgtacttcc
    13561 tgtcacgtta tgccagttct tgcgattatc gcgggcggta taaatatacc gactattaaa
    13621 tgtccgaata tcactgcgat aacccatttg ctgaaactgt tggcgaatat aatcggcaga
    13681 taacatttct gctggagttc cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc
    13741 ctgggtatta gcaaaatcgc caggtttagg tgacgaggcg tgtacgggga gaataaagca
    13801 tacgccgagc gccagggcag cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta
    13861 aataccatgc aaattttttt accgccatag tatgaaactg ccgctgcgct aaaacaattt
    13921 caaatcttcc taaacgcccg aaatccggtg ccttaagcac tttttgatat tagctttgcc
    13981 aaatcgttat tccgttaagg aactactcat tctaattggt aatttcattc gttctcttac
    14041 gctccctata gtcgaaacat ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta
    14101 tggctcaaaa acgacttact cacctgcggc aactggaggc ggaaagcatc cacattattc
    14161 gcgaggtggc ggcagaattc tcaaatccgg tgatgctcta ctctatcggt aaagattcca
    14221 gcgtcatgct gcatctggcg cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc
    14281 tgcatgtcga taccggctgg aaattccgcg agatgtatga gttccgcgat cgtacggcga
    14341 aagcctacgg ctgcgagctg ctggtgcata aaaacccgga aggcgtggcg atggggatta
    14401 atccattcgt gcacggcagc gcgaaacata ccgatattat gaaaactgaa ggcctgaaac
    14461 aggcgctgaa caaatacggt tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga
    14521 aatcccgcgc taaagagcga atctactctt tccgtgaccg cttccatcgc tgggatccga
    14581 aaaatcagcg cccggagctg tggcacaact acaacgggca aattaacaaa ggcgaaagca
    14641 tccgcgtctt cccgctctct aactggaccg agcaggatat ctggcaatac atctggctgg
    14701 aaaatatcga cattgtgccg ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg
    14761 gtatgttgat gatgattgat gacaaccgta tcgacctgca accgggcgaa gtgatcaaaa
    14821 aacggatggt gcgtttccgt acgctgggct gctggccgct gaccggtgcg gtggagtcaa
    14881 atgcacaaac actgccggaa atcatcgaag agatgctggt ttccaccacc agtgaacgtc
    14941 agggtcgcgt gattgaccgc gaccaggcgg gatccatgga gctgaaaaaa cgtcaggggt
    15001 atttttaaga tgaacaccgt acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc
    15061 tggatgattg cgcaacaaca taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat
    15121 gacggcaaaa gtaccctgat tggtcgtctg ctgcacgata cccgccaaat ctacgaagat
    15181 cagctctcat cgctgcataa cgacagtaag cgtcacggaa cccagggcga aaagctggat
    15241 ctggctctgc tggtggacgg cctgcaagct gagcgcgaac agggcatcac tattgatgtg
    15301 gcctaccgct atttctctac cgagaagcgt aaatttatta tcgccgacac cccagggcac
    15361 gagcagtaca ctcgcaatat ggcgactggc gcatcgacat gtgaactggc gatcttactg
    15421 attgatgccc gtaaaggcgt gctcgatcaa acccgtcgtc acagttttat ctccacactg
    15481 ttggggatca aacatctggt cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa
    15541 gagacgttca cccgtattcg tgaagattat ctgacctttg ccgggcagct gccgggtaat
    15601 ctggatatcc gctttgtgcc gctctccgca ctggaaggtg acaacgtggc ttcgcaaagt
    15661 gaaagtatgc cgtggtacag cggtctgaca ctgctcgaag tgctggaaac cgtggagatc
    15721 cagcgagtgg tggatgccca gccaatgcgc ttcccggtgc agtacgttaa ccgcccaaat
    15781 ctcgattttc gtggctacgc cggaatgctg gcatccggtc gcgtggaagt cgggcaacgt
    15841 gtaaaagtgc tgccctctgg tgtggaatca aacgtcgcgc ggatcgtgac ttttgatggt
    15901 gatcgcgaag aagtctttgc cggagaagcg atcaccctgg tgctgacgga tgagatcgat
    15961 atcagccgtg gtgatctgct gctggcggca gacgaagcgt taccggcggt gcagagtgcg
    16021 tcggtggatg tggtatggat ggcggaacag ccgctttccc cgggccagag ttatgacatc
    16081 aaaattgccg gtaagaagac gcgtgctcgt gttgatggca ttcgttatca ggttgatatt
    16141 aataacctta cccaacgcga agttgaaaac ctgccgctga acggcatcgg cctggtggat
    16201 ctcacttttg acgagccact ggtgttagat cgctatcagc aaaatccggt taccggtggg
    16261 ctgattttta tcgatcgcct gagcaatgtg accgtgggtg ccggtatggt gcacgagcca
    16321 gttagccagg caacagctgc gtcatctgaa ttcagtgcat tcgaactgga attgaatgcc
    16381 ctggttcgtc gccactttcc gcactggggc gcgcgcgatt tgctggggga taaataatgg
    16441 cgctgcatga cgaaaacgtc gtctggcata gtcatccggt cactgtgcaa caacgcgaac
    16501 tacaccacgg tcatcgtggt gtagtgctgt ggtttaccgg cctctccggg tccggtaaat
    16561 caacggtcgc cggggcgctg gaggaggcgt tacataaact cagcgtcagt acgtatctgc
    16621 tggatggcga caatgttcgc cacggattat gcagcgatct cggttttagc gatgccgatc
    16681 gtaaagagaa tatccgtcgc gtcggtgaag tggcgaattt gatggttgaa gccggactgg
    16741 tggtgctgac cgcatttatc tcgccacacc gcgccgaacg ccagatggtt cgcgaacgcg
    16801 taggagaagg gcgctttatt gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc
    16861 gcgatccaaa aggcttatat aagaaagcgc gtgccggtga actgcgcaac tttacgggaa
    16921 tagattccgt ttacgaagcg cctgaatcgg cagaaattca tctcaatggt gaacaattag
    16981 taacaaattt ggtacagcaa ttattagatc tgttgagaca gaacgatatt atcagatcct
    17041 gagacaccac cgggtttcca tgcccggttc gtcaaagtaa caggatcaaa tatgcgtaat
    17101 agccataaca ttacactaac aaataacgac agccttactg aggatgaaga aactacatgg
    17161 tcactgcctg gtgccgtggt cggttttatc tcctggttat ttgcgctggc gatgccgatg
    17221 ttgatttatg gctctaacac gctgttcttc tttctctaca cctggccttt ctttctggca
    17281 ctgatgcctg tcgcggtagt ggtggggatt gcgctgcatt cattgatgga cggaaagcta
    17341 cgctacagta ttgttttcac cctggtgaca gtaggcatta tgtttggcgc actgtttatg
    17401 tggctactgg gctaatttgt actttcttgt ctcctctgtt cataattcaa accgtaacta
    17461 ataatgagat tatgttctgc acgccctggg tatacgtaac aatgggcaaa tgtggtacat
    17521 ttgcccgcgt tgtcgcggta tccccaacag aggatgtaga gtcgtcttcg gatgcatggg
    17581 atgatgatgc agtttttcag ggggcaggat gggtaaacta acgctgctgt tgctggctat
    17641 tctggtctgg ctacagtatt cgctgtggtt cggtaagaac ggtatacatg actatacccg
    17701 cgtcaatgat gatgtggcgg cactgcaagc tacaaacgcg aaacttaaag cgcgaaacga
    17761 tcaacttttt gccgaaattg acgatctcaa tggcggccag gaggcgctcg aagagcgtgc
    17821 gcgtaatgaa ctcagcatga ccaggccggg cgaaactttt tatcgtctgg tgcctgacgc
    17881 gtcgaagcgc gcacagtctg cggggcaaaa caatcgataa atcagcccgg gaattaacat
    17941 ggcaaccact catttggatg tttgcgccgt ggttccggcg gccggatttg gccgtcgaat
    18001 gcaaacggaa tgtcctaagc aatatctctc aatcggtaat caaaccattc ttgaacactc
    18061 ggtgcatgcg ctgctggcgc atccccgggt gacacgtgtc gtcattgcca taagtcctgg
    18121 cgatagccgt tttgcacaac ttcctctggc gaatcatccg caaatcaccg ttgtagatgg
    18181 cggtgatgag cgtgccgatt ccgtgctggc aggtctgaaa gccgctggcg atgtgcagtg
    18241 ggtattggtg catgacgccg ctcgtccttg tttgcatcag gatgaccttg cgcgattgtt
    18301 ggcgttgagc gaaaccagcc gcacgggagg gatcctcgcc gcaccagtgc gcgatactat
    18361 gaaacgtgcc gaaccgggca aaaatgccat tgctcatacc gttgatcgca acggcttatg
    18421 gcacgcgctg acgccgcaat ttttccctcg tgagctgtta catgactgtc tgacgcgcgc
    18481 tctaaatgaa ggcgctacca ttaccgacga agcctcggcg ctggaatatt gcggatttca
    18541 tccgcagttg gtcgaaggcc gcgcggataa cattaaagtc acgcgcccgg aagatttggc
    18601 actggccgag ttttacctca cccgaaccat ccatcaggag aatacataat gcgaattgga
    18661 cacggttttg acgtacacgc ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc
    18721 attccttacg aaaaaggatt gctggcgcat tctgatggcg acgtggcgct tcatgcgttg
    18781 accgatgcat tgcttggcgc ggcggcgctg ggggatatcg gcaaactgtt cccggatacc
    18841 gatccggcat ttaaaggtgc cgacagccgc gagctgctac gcgaagcctg gcgtcgtatt
    18901 caggcgaagg gttataccct tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag
    18961 atgttgccgc acattccaca aatgcgcgtg tttattgccg aagatctcgg ctgccatatg
    19021 gatgatgtta acgtgaaagc cactactacg gaaaaacttg gatttaccgg acgtggggaa
    19081 gggattgcct gtgaagcggt ggcgctactc attaaggcaa caaaatgatt gagtttgata
    19141 atctcactta cctccacggt aaaccgcaag gcaccgggct gctgaaagcc aatccggaag
    19201 actttgtggt ggtggaagat ttgggctttg agcctgatgg tgaaggtgag catattctgg
    19261 ttagaatcct caaaaacggc tgcaataccc gttttgtggc ggatgcactg gcgaaattcc
    19321 tgaaaattca ttcccgtgaa gtcagcttcg ctgggcaaaa agacaaacat gctgtaacgg
    19381 aacagtggtt atgtgctcgc gtgccgggca aggaaatgcc cgatctgagc gcctttcaac
    19441 tggaaggctg ccaggtgctg gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc
    19501 tgaaaggtaa cgcctttacc ctggttctgc gcgaagtgag caatcgcgat gacgttgaac
    19561 aacgtctgat cgatatttgc gtaaaaggtg taccgaacta cttcggtgcc caacgttttg
    19621 ggattggcgg tagcaacttg cagggcgcgc tgcgctgggc gcaaaccaat actccggtgc
    19681 gcgatcgcaa taaacggagt ttttggttgt cggcagcccg cagtgcgttg tttaatcaga
    19741 ttgttgctga gcgcctcaaa aaagcagacg ttaatcaagt tgttgacggc gatgcgctac
    19801 aattagccgg acgtggtagc tggtttgtag caaccaccga agaactggcg gaattacagc
    19861 gtcgcgtcaa cgataaagag ctgatgataa ccgccgcatt gccgggcagt ggcgaatggg
    19921 gaactcagcg tgaagcgctg gcattcgaac aagcagctgt cgccgcagaa actgaattac
    19981 aagctttact ggtgcgcgaa aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc
    20041 aacaattaag ctggaactgg tgggatgacg tcaccgtaga gatccgtttc tggcttccgg
    20101 cgggtagttt tgcaaccagc gttgtcaggg aacttatcaa cacaacaggt gattatgcgc
    20161 atattgctga gtaattatga cggggtacat gcacccggta tacaaacgct ggcgaaagcc
    20221 ttgcgtgagt ttgctgacgt tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca
    20281 aattctctga cactggaatc ctccctgcgc acgtttacct ttgaaaatgg tgatattgct
    20341 gtgcaaatgg gaaccccgac cgattgtgtc tatcttggcg tgaatgctct gatgcgtccg
    20401 cgcccggaca ttgttgtgtc cggaattaac gccgggccga atctggggga tgatgttatt
    20461 tattccggta cggtagccgc cgcgatggaa ggccgtcatt taggttttcc ggcgctggcc
    20521 gtctcgcttg acgggcataa acattacgac actgccgcgg cggtaacctg ttcaattttg
    20581 cgcgcactgt gtaaagagcc gctgcgcacc ggacgtattc ttaatattaa cgttcaggat
    20641 ttacccttgg atcaaatcaa aggtattcgc gtgacgcgct gcggtacacg acatccggca
    20701 gatcaggtga tcccgcagca agatccgcgc ggcaatacgc tgtactggat tggcccgccg
    20761 ggcggtaaat gtgatgccgg tccggggacc gattttgctg cggtagatga gggctatgtc
    20821 tccatcacgc cgctgcatgt ggatttaact gcgcatagcg cgcaagatgt ggtttcagac
    20881 tggttaaaca gcgtgggagt tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg
    20941 atcaattacg tgcgcaaggt attcaggatg agcaggtgct gaatgcactt gccgccgtgc
    21001 cgcgtgaaaa attcgttgat gaagcgtttg aacaaaaagc ctgggacaat atcgccttgc
    21061 cgataggtca ggggcagaca atttcgcagc cgtatatggt ggcgcgaatg accgaattac
    21121 tcgagctgac gccgcagtcg cgggtgctgg aaattggcac tggttcggga tatcaaacgg
    21181 caatcctggc gcatcttgtc cagcatgttt gctcggttga acggattaaa ggcttgcagt
    21241 ggcaggcacg tcgccgcctg aaaaatcttg atttacataa tgtttcaacc cgtcatggtg
    21301 atggatggca aggttggcag gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac
    21361 cgccggaaat tccaactgcg ctaatgacgc agctggacga aggcgggatt ctcgtcttac
    21421 ccgtagggga ggagcaccag tatttgaaac gggtgcgtcg tcggggaggc gaatttatta
    21481 tcgataccgt ggaggccgtg cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg
    21541 tgaggaaata cctggatttt tcctggttat tttgccgtag gtcagcgtat cgtgaacatc
    21601 ttttccagtg ttcagtaggg tgccttgcac ggtaattatg tcactggtta ttaaccaatt
    21661 tttcctgggg gataaatgag cgcgggaagc ccaaaattca ccgttcgccg cattgcggct
    21721 ttgtcactgg tttcgctatg gctggcaggc tgttctgaca cttcaaatcc accggcaccg
    21781 gtcagctccg ttaatggcaa tgcgcctgca aatactaatt ctggtatgtt gattacgccg
    21841 ccgccgaaaa tggggacgac gtctacagcg cagcaaccgc aaattcagcc ggtacagcag
    21901 ccacaaattc aggctactca acaaccgcaa atccagccag tgcagccagt agctcagcag
    21961 ccggtacaga tggaaaacgg acgcatcgtc tataaccgtc agtatgggaa cattccgaaa
    22021 ggcagttata gcggcagtac ctataccgtg aaaaaaggcg acacactttt ctatatcgcc
    22081 tggattactg gcaacgattt ccgtgacctt gctcagcgca acaatattca ggcaccatac
    22141 gcgctgaacg ttggtcagac cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc
    22201 ggaaatgcca ttacccaggc cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa
    22261 aattccaccg ttgctgttgc gtcgcaaccg acaattacgt attctgagtc ttcgggtgaa
    22321 cagagtgcta acaaaatgtt gccgaacaac aagccaactg cgaccacggt cacagcgcct
    22381 gtaacggtac caacagcaag cacaaccgag ccgactgtca gcagtacatc aaccagtacg
    22441 cctatctcca cctggcgctg gccgactgag ggcaaagtga tcgaaacctt tggcgcttct
    22501 gaggggggca acaaggggat tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc
    22561 gcagatggcc gcgttgttta tgctggtaac gcgctgcgcg gctacggtaa tctgattatc
    22621 atcaaacata atgatgatta cctgagtgcc tacgcccata acgacacaat gctggtccgg
    22681 gaacaacaag aagtgaaggc ggggcaaaaa atagcaacca tgggtagcac cggaaccagt
    22741 tcaacacgct tgcattttga aattcgttac aaggggaaat ccgtaaaccc gctgcgttat
    22801 ttgccgcagc gataaatcgg cggaaccagg cttttgcttg aatgttccgt caagggatca
    22861 cgggtaggag ccaccttatg agtcagaata cgctgaaagt tcatgattta aatgaagatg
    22921 cggaatttga tgagaacgga gttgaggttt ttgacgaaaa ggccttagta gaagaggaac
    22981 ccagtgataa cgatttggcc gaagaggaac tgttatcgca gggagccaca cagcgtgtgt
    23041 tggacgcgac tcagctttac cttggtgaga ttggttattc accactgtta acggccgaag
    23101 aagaagttta ttttgcgcgt cgcgcactgc gtggagatgt cgcctctcgc cgccggatga
    23161 tcgagagtaa cttgcgtctg gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg
    23221 cgttgctgga ccttatcgaa gagggcaact tggggctgat ccgcgcggta gagaagtttg
    23281 acccggaacg tggtttccgc ttctcaacat acgcaacctg gtggattcgc cagacgattg
    23341 aacgggcgat tatgaaccaa acccgtacta ttcgtttgcc gattcacatc gtaaaggagc
    23401 tgaacgttta cctgcgaacc gcacgtgagt tgtcccataa gctggaccat gaaccaagtg
    23461 cggaagagat cgcagagcaa ctggataagc cagttgatga cgtcagccgt atgcttcgtc
    23521 ttaacgagcg cattacctcg gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc
    23581 tggacatcct ggccgatgaa aaagagaacg gtccggaaga taccacgcaa gatgacgata
    23641 tgaagcagag catcgtcaaa tggctgttcg agctgaacgc caaacagcgt gaagtactgg
    23701 cacgtcgatt cggtttgctg gggtatgaag cggcaacact ggaagatgta ggtcgtgaaa
    23761 ttggcctcac ccgtgaacgt gttcgccaga ttcaggttga aggcctgcgc cgtttgcgcg
    23821 aaatcctgca aacgcagggg ctgaatatcg aagcgctgtt ccgcaagtaa gtaagcatct
    23881 gtcagaaagg ccagtctcaa gcgaggctgg cctttttctt ttgtttggga agcttgtgtt
    23941 tttgtcgcct ggataagaca cgtcagcgtc ctacccggca atcagcattc tatgtgctca
    24001 tcatcttgcg caccaactgc ataaacagct cctgctcttc tccactcaaa cgccccaaaa
    24061 attcctcatc cacgctgtca ccaataggta tcgccgcagc aagtagcttt tctccttcgg
    24121 cagtcagcca gacaaagcgt cgccgcttgt cagcgacgtc atgttcacgt ctgactaagc
    24181 cacgattttc cattcttgcc aacatttctg ctagcgttgc tttggtgctg actgctgctt
    24241 ctatcagtgc cacctgttca ataccaggtt tatcagcaat ggcgcgcatc accgcgtact
    24301 gtggtttggt caggtcaggt aactcgtgct gccagcgagc cgtgtgctgc tgaaaaagct
    24361 gtcgtaactg atggaacgct ttatttcgta acgccatgta aactccgggc taattctctg
    24421 tcgctatcat agcggttttt tagtgacgaa gaagatgttg ttacttttca atccttgccg
    24481 ctgtggaaat gaaggagtat gttaataata acgttcgtat acgaataatt aagagaataa
    24541 gcaatgaaac tgatcgtcgg gatgacaggg gctaccggtg cgcctcttgg tgtggcatta
    24601 ctgcaagcgc tgcgtgagat gccgaatgtc gagactcatc tggtgatgtc gaagtgggcg
    24661 aaaaccacca ttgaactgga aacgccttac agcgctcgcg atgttgctgc cctcgcagac
    24721 ttcagccata acccggcgga tcaggcggcg atcatctcat ccggttcttt tcgtaccgac
    24781 ggcatgatcg ttattccgtg cagtatgaaa acgctcgccg gtatccgcgc tggttacgcc
    24841 gatggcctgg tagggcgcgc ggcggacgtc gtgctcaaag aaggccgcaa actggtgctg
    24901 gtgccgcgtg aaatgccgct tagcaccatc catctcgaaa atatgctcgc actttcacgc
    24961 atgggcgtgg cgatggtgcc gccgatgcct gccttttata accatcccga aacggtagat
    25021 gacattgtcc accatgtggt agcccgcgtg ctggatcaat ttggcctcga acatccccac
    25081 gccaggcgct ggcaaggatt gccgcaggcc cggaattttt ctcaggagaa tgaataatgg
    25141 catttgatga tttacgcagc tttttacagg cgcttgatga ccacggccag ttactgaaaa
    25201 tcagcgaaga agtgaacgct gagccggatc tggcagcagc agctaacgcc accgggcgta
    25261 tcggcgacgg cgcgcccgcg ctgtggtttg ataatattcg cggctttacc gatgcccgcg
    25321 tggcgatgaa taccatcggt tcctggcaga accacgcgat ttcccttggc ctgccaccaa
    25381 acaccccggt caaaaagcag attgatgagt ttatccgccg ctgggataac ttcccgattg
    25441 ccccggagcg ccgcgccaat ccagcctggg cgcagaacac cgtcgatggc gaagagatca
    25501 acctgttcga tattctgccg ctgtttcgcc tgaatgatgg cgatggcggt ttctatctcg
    25561 acaaagcgtg cgtggtttcc cgcgatccgc tcgacccgga taacttcggc aagcagaacg
    25621 tcggcatcta ccgcatggaa gtgaagggca agcgtaagct cggcctgcaa ccggtgccga
    25681 tgcacgatat cgccctgcat ctgcataaag cagaagagcg cggtgaagat ctgccgattg
    25741 cgatcacgct cggtaacgat ccgatcatca cgctgatggg ggccacgccg ctgaaatacg
    25801 atcagtctga gtatgaaatg gcaggcgcgc tgcgtgaaag cccgtacccg atcgccaccg
    25861 ctccgctgac cggttttgat gtgccgtggg gttcagaagt gatcctcgaa ggggttatcg
    25921 aaagccgtaa acgcgaaatc gaagggccgt tcggtgagtt taccgggcac tactccggtg
    25981 ggcgcaacat gaccgtggtg cgcatcgata aagtctctta tcgcagcaaa ccgattttcg
    26041 aatcactcta tctcggtatg ccgtggaccg aaatcgacta cctatgacta ggtaatttat
    26101 tcaagataac cgggaagaga ttaaaaaaga cgggaataga ttggaatcaa caagttagga
    26161 actcaaggtt ttcaagataa agcgagtcca aaaatgttaa acggttcgat atcaataaaa
    26221 cattcaagat aactcacagg ttcttacaat agcctttaaa gaccgttcaa aaacgattca
    26281 ggtgtttatt atagcgctga tgggtgcaag cgccaaaacc aatgtaattt tagcacagct
    26341 aggcacttgc actgggttat gcgctggctg ctttctgagg tagatttaag tgatcgccct
    26401 tagcttcgga caacgcgtaa atctctctaa ccttttcgtc tggcaaatta ccaaggctta
    26461 acgctatggc tctacgaact ccaactagag cgtgcaactc ttggatgtgc tcatccagaa
    26521 gaatgagtaa ttgctccgct ccttttctcc caagaacatc agctacttgt tgtttttctt
    26581 gtggtttaag aaatgacaag aacattagaa tttgagcatc aacattatcc gcattctctt
    26641 ttttgggttc tttggaatct tttagttcaa catcaccctc tcccgtcatg agccattcca
    26701 aggtaacatt ttcgctttct gctatctttt ttaactgata cgggcgtggt atcggtgcgt
    26761 aatcccgcct gttgtaataa ttttttaaag tgccttcatt gatcccccat gcccgagcag
    26821 ccgcatttct tgaagggtat cgttctacta acctaaagat tctttcgata atcggttctt
    26881 ttccatcggc aggaaaagaa ccttggggtg cttttctcat tcttggttcc tttctatcta
    26941 attcattgat aaataataaa attaaaaaat aaaccataaa aacaccaaag aaccctttgc
    27001 agggaatctt tggtatctta atctttatcc atacggatat ccgaccggat atccgcgcgg
    27061 gtaattttgt aggttaaagg aaagatgaga caaagagaag atagtgaccg cgattggcct
    27121 tcgctgaaga ttcagctggc gctcaaagat aacgggtggt cttccttccg ggagctttca
    27181 ctccatgctg gttatgctca ggattcactg aaagcggtag ttcgcacacc aaacaaaaaa
    27241 tacgaacgcg taatttctga cgctataggt gtaccgccag aaatcatctg gcccagccgg
    27301 tatcgtaacg atggatttat caggaaggcg tcgtgatatg cagtcgtacg ttaccgtaaa
    27361 tgacctgctc ggcgtaccag gaatgccagc caccacaaaa ggcatccgac aggcgttaca
    27421 gcgtttttct cgtgatcttg gtgacgttag tcgccgtcgg gaaggcacaa aagccattga
    27481 gtaccacatt gattgtctgc ctgaaataac acgaaaggca ctgcgtgaac gctatgtgga
    27541 acagctggtg gctacagaga acaatgtttc tgaagtgaaa gctgttacca gaaaaacacg
    27601 caatcctgac gctgtccagg caatcgaagc atatcgcggt tctccacaac tgatggaaga
    27661 acgcctgaat gcgctgaccg aaaaccagcg ctgggtatct gaagcaagag ctgcgctggt
    27721 ggtggaagtg ctgaagctgg aaagcgccgg taaccccggg cgactgaaag ccattaactt
    27781 tcttgttgaa aaagcccgta aaggtgagct gccggagcgc ctgcaacagg ccgcagttaa
    27841 cgccaatgca aaacgtggcg ctaatcgtac tatcagccgc gatccacttt accagtgggt
    27901 tctgaaatac aaccagtcgc agaacgccgc cgaacggctg ctgttactgg caccgggaaa
    27961 acgcgacgaa ataaaaccgg aagaaatcag ctggctgcct gaatttctgg cgcagtatcg
    28021 ccaggtgaac ggcagaccca tgtcagaagc ctacgaggat tttgtcgcag aatggcagcg
    28081 tcgccatgcg gacgaaccgt acatgctgga ggtgatgcct tcctatgatg ttgtccggta
    28141 tgccatgaaa aaactgccgg aagtggtgaa acaaaaaggg cgcgttaccg gcagtgaata
    28201 ccgtcagctt gaaggattca cccgccgcga ctggacagct atgccggtga attatgtctg
    28261 gattggtgac ggtcacggta tgaagctgaa atgtgcgcat ccgatccacg ggcgtccatt
    28321 ctcaccggaa gtcacgtttg tgatcgatgg tggaacacgg tttgttgttg gctggagtct
    28381 tgaccttgcc gaaaacgtgt tcgccgttgc cggtgcgata cagcacggca ttcgcaacca
    28441 cggaaaaccg ttcctgtatt actcggataa cggttccggt gaaacggctg acatgctgga
    28501 caaagaggtc gtgggtattc tgccacgcct ggggattaaa caccctaccg gtattgccgg
    28561 taatccacag ggacgcggca ttattgaacg cctgaaccgg acactgccga tgcgtatagc
    28621 caggaggtat cgcacctatt ttggcaaggg tgctgaccgg gaatcgttgc gtgtacttaa
    28681 ccgtgatctg cgttcagcgt ttaacgccct gcaacaggac aagcctctga acgaccgcca
    28741 gaaagcggca atgcgcgagc tgccatcgtg ggcggagctt attgaggcta ttcgtgaagg
    28801 cgtggaatgg tacaacaacc gcccgcattc tgagctgccg atgaaaccga acggacggca
    28861 ttacagcccg acagagttca gaaaaaaacg ccaggcagaa gaagacaccg aaattgagtg
    28921 gctgagtgat cttgagctgc gcgacatgtt ccgcccaatg gtggaacgac cggtaagacg
    28981 ctgcgaaatt cagtggctga acaatattta ctacgcgccg gagttgcgtg acgaacacgg
    29041 tcgcaaagtg cttatcagtt atgacattca tgacgccgag cgaattacgg tacgtcgcaa
    29101 ggacggcagc tttatctgtg aggcgatatg gaacggcaat aaacgcgcag cctttgctgt
    29161 cagtgctgaa tatcacaaac aacagcagcg tattaaagga atgcgcaaac gcgctgagga
    29221 aaaaattcgt gatgctgaag atgaaggtat tcagattctg gaacacaaac aggctgaacc
    29281 ctggctgagt aatgtttatc gcccggttgg taatgtggtg gcagttcagc aaccggaata
    29341 tgaagaagag catgacgaag aatttgaacg cg
//
LOCUS       DAJTRN010000048        29033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRN010000048 DAJTRN010000000
VERSION     DAJTRN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO328_002150"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO328_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5930708.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO328_002151"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO328_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5930709.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO328_002152"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO328_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5930710.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO328_002153"
     CDS             4843..5082
                     /locus_tag="OO328_002153"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930711.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO328_002154"
     CDS             5092..5262
                     /locus_tag="OO328_002154"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5930712.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO328_002155"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO328_002155"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5930713.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO328_002156"
     CDS             6724..6990
                     /locus_tag="OO328_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5930714.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO328_002157"
     CDS             7084..7773
                     /locus_tag="OO328_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5930715.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO328_002158"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO328_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5930716.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO328_002159"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO328_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5930717.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO328_002160"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO328_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5930718.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO328_002161"
     CDS             complement(10475..12193)
                     /locus_tag="OO328_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5930719.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO328_002162"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO328_002162"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5930720.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO328_002163"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO328_002163"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5930721.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO328_002164"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO328_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5930722.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO328_002165"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO328_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5930723.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO328_002166"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO328_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO328_002167"
     CDS             18432..18746
                     /locus_tag="OO328_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5930724.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO328_002168"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO328_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5930725.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO328_002169"
     CDS             20570..21862
                     /locus_tag="OO328_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5930726.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO328_002170"
     CDS             complement(22016..23665)
                     /locus_tag="OO328_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5930727.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO328_002171"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO328_002171"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5930728.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO328_002172"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO328_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5930729.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO328_002173"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO328_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5930730.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO328_002174"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO328_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..>29033
                     /locus_tag="OO328_002175"
     CDS             28753..>29033
                     /locus_tag="OO328_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5930731.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7279 a         7775 c         7350 g         6629 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctg
//
LOCUS       DAJTRN010000049        28565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRN010000049 DAJTRN010000000
VERSION     DAJTRN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1757)
                     /gene="yccS"
                     /locus_tag="OO328_002176"
     CDS             complement(<1..1757)
                     /gene="yccS"
                     /locus_tag="OO328_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5930732.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDA"
     gene            complement(1767..2213)
                     /locus_tag="OO328_002177"
     CDS             complement(1767..2213)
                     /locus_tag="OO328_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5930733.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2336..4390
                     /gene="helD"
                     /locus_tag="OO328_002178"
     CDS             2336..4390
                     /gene="helD"
                     /locus_tag="OO328_002178"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5930734.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4422..4880)
                     /gene="mgsA"
                     /locus_tag="OO328_002179"
     CDS             complement(4422..4880)
                     /gene="mgsA"
                     /locus_tag="OO328_002179"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5930735.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(4976..5638)
                     /locus_tag="OO328_002180"
     CDS             complement(4976..5638)
                     /locus_tag="OO328_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5930736.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            5811..6224
                     /locus_tag="OO328_002181"
     CDS             5811..6224
                     /locus_tag="OO328_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5930737.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6269..6586)
                     /gene="hspQ"
                     /locus_tag="OO328_002182"
     CDS             complement(6269..6586)
                     /gene="hspQ"
                     /locus_tag="OO328_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5930738.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6644..7834)
                     /gene="rlmI"
                     /locus_tag="OO328_002183"
     CDS             complement(6644..7834)
                     /gene="rlmI"
                     /locus_tag="OO328_002183"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5930739.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            7929..8207
                     /gene="yccX"
                     /locus_tag="OO328_002184"
     CDS             7929..8207
                     /gene="yccX"
                     /locus_tag="OO328_002184"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5930740.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8204..8533)
                     /gene="tusE"
                     /locus_tag="OO328_002185"
     CDS             complement(8204..8533)
                     /gene="tusE"
                     /locus_tag="OO328_002185"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5930741.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8624..9283)
                     /gene="yccA"
                     /locus_tag="OO328_002186"
     CDS             complement(8624..9283)
                     /gene="yccA"
                     /locus_tag="OO328_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5930742.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASMV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9490..9577)
                     /locus_tag="OO328_002187"
     tRNA            complement(9490..9577)
                     /locus_tag="OO328_002187"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9541..9543),aa:Ser,seq:tga)
     gene            10003..11121
                     /gene="hyaA"
                     /locus_tag="OO328_002188"
     CDS             10003..11121
                     /gene="hyaA"
                     /locus_tag="OO328_002188"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5930743.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11118..12911
                     /gene="hyaB"
                     /locus_tag="OO328_002189"
     CDS             11118..12911
                     /gene="hyaB"
                     /locus_tag="OO328_002189"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5930744.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            12930..13637
                     /gene="hyaC"
                     /locus_tag="OO328_002190"
     CDS             12930..13637
                     /gene="hyaC"
                     /locus_tag="OO328_002190"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5930745.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13634..14221
                     /locus_tag="OO328_002191"
     CDS             13634..14221
                     /locus_tag="OO328_002191"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5930746.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14218..14616
                     /gene="hyaE"
                     /locus_tag="OO328_002192"
     CDS             14218..14616
                     /gene="hyaE"
                     /locus_tag="OO328_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5930747.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14613..15470
                     /locus_tag="OO328_002193"
     CDS             14613..15470
                     /locus_tag="OO328_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5930748.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15604..17148
                     /gene="appC"
                     /locus_tag="OO328_002194"
     CDS             15604..17148
                     /gene="appC"
                     /locus_tag="OO328_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5930749.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17160..18296
                     /gene="appB"
                     /locus_tag="OO328_002195"
     CDS             17160..18296
                     /gene="appB"
                     /locus_tag="OO328_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5930750.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18308..18400
                     /gene="cbdX"
                     /locus_tag="OO328_002196"
     CDS             18308..18400
                     /gene="cbdX"
                     /locus_tag="OO328_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5930751.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18480..19778
                     /gene="appA"
                     /locus_tag="OO328_002197"
     CDS             18480..19778
                     /gene="appA"
                     /locus_tag="OO328_002197"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5930752.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAPF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(19893..22059)
                     /gene="etk"
                     /locus_tag="OO328_002198"
                     /pseudo
     CDS             complement(19893..22059)
                     /gene="etk"
                     /locus_tag="OO328_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22079..22525)
                     /gene="etp"
                     /locus_tag="OO328_002199"
                     /gene_synonym="yccY"
     CDS             complement(22079..22525)
                     /gene="etp"
                     /locus_tag="OO328_002199"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5930753.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22513..23652)
                     /locus_tag="OO328_002200"
     CDS             complement(22513..23652)
                     /locus_tag="OO328_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5930754.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(23698..25764)
                     /locus_tag="OO328_002201"
     CDS             complement(23698..25764)
                     /locus_tag="OO328_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003860916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5930755.1"
                     /translation="MAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQMPNARIAPEG
                     EFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGDQSYKDKSFDF
                     KLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMAWGYAGNAGNI
                     TNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPLRLKLEYDGNN
                     YQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTNFNDLRPALRD
                     TPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMSGQQYKYRDSR
                     EAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAGTAPGKSEQLQ
                     QQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGLMSSARYWFTD
                     HLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLNNLQANYFADL
                     GNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWDNMMRFTDYST
                     PTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVGVWAAISNVSK
                     DDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRKYQLDPMTAER
                     EVPVGQ"
     gene            complement(25816..26562)
                     /locus_tag="OO328_002202"
     CDS             complement(25816..26562)
                     /locus_tag="OO328_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5930756.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26559..27203)
                     /locus_tag="OO328_002203"
     CDS             complement(26559..27203)
                     /locus_tag="OO328_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5930757.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27310..27615)
                     /locus_tag="OO328_002204"
     CDS             complement(27310..27615)
                     /locus_tag="OO328_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930758.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28057..28269)
                     /gene="cspH"
                     /locus_tag="OO328_002205"
     CDS             complement(28057..28269)
                     /gene="cspH"
                     /locus_tag="OO328_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5930759.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6918 a         7235 c         7512 g         6900 t
ORIGIN      
        1 gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt atcacgcccc
       61 tgatggtatt gctccagtat ggcatcgaga tagcgacagt tggcctctgt ggcgcgttcg
      121 agcattcgcg gcagattgcg aaacttccag tcaggccaga tgtagctcac tgccgcccac
      181 gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgctacttc aaaaccttca
      241 cccagtaagt taaaacacag tagcaccaaa agtgtgatga acatcgttgc atgagcgtat
      301 tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac cagctgccct
      361 tccagtgatg gcacaaacca cagcacagga atgccaatcg cgatacctac cagcgtacca
      421 ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa
      481 cttgtcagca agatccaata cccgtgatgc attccggtta tttgaatgat ggcgtagccg
      541 aagcacaaca ccagcgacat tcttaccgca tgacggaaga gggcggattc cggcgtgaag
      601 tgacggctaa gacgcagcca gatatcactc aatccgtgcg ggctgtcatc agcgagctca
      661 ttttcgtcat tattacgggg tagtgtctgg gcctgttctg attcaattgt tgccagttgg
      721 gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa atcggcgggt
      781 gcgccgttat cgcgtatccg ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa
      841 tgcggatcat gttgataaga ctgacgcaac aaaatacagc gtgacagttg ctggcacgcc
      901 tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt
      961 tcacgcaatg tttgatactg aatatgagaa gagctggcac gctcgtgaat atcctgtgcg
     1021 acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg
     1081 gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gctgaccgtt ggcgagagcc
     1141 aaatcgtaca gcggtgcctg gctttcatct tcaatatcag gatcaaacat gcgcgacttg
     1201 agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg
     1261 accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc accggccagc
     1321 agatacatcg gctgctgata ccagtgctca tacagtgatg ttcccaacat agtgtaaatg
     1381 gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa
     1441 atgaagccgc tggtagagag cgttaagcca atcgcaaata gccagggcca gggaaacagc
     1501 aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag gttacgcaaa
     1561 cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc cagcgttagc
     1621 ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc acaaagcgca
     1681 ataaaaatac gcgcgtaata cagccaggcg ctgttccagg tatagcgttt gagcaaagga
     1741 cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt ggcttcacgc
     1801 gcagcttgcg ctgtttctac cgataccacg cgacgaccaa ccggccatag tgcaatagcg
     1861 gcaattttaa agttcgcaat gccgacagga atgccaatga ttgaaataca ttgtgcgatg
     1921 cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca gaaaatattc
     1981 aataccgtac cgccagtatt cagcaacaca tttttgccag ccgggttcag ttcatcgaca
     2041 tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca gcaggatcgt
     2101 gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag ccagcccaga
     2161 gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg cataaaacct
     2221 cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg gctggagcac
     2281 gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca gagtcatgga
     2341 actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc gggcggtgat
     2401 cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattc ctttcaatca
     2461 attactggcg attcactgta agcgcggtct ggtatggggc gagctggaat ttgtactgcc
     2521 ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc gagacgcagc gtttttacca
     2581 tcatcttgat gctcactggc ggcggtggag tggcgagatg agcgaaattg cgtctggtgt
     2641 tttacgccag caactggatt tgattgccac gcgcactgga gaaaataaat ggctgacgcg
     2701 tgagcaaacc tctggcgttc agcaacaaat ccgccaggct ttgtcggcgt tgccgttgcc
     2761 ggttaaccga ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat gtcaggcttg
     2821 gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg aagccatgct
     2881 taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc
     2941 ccgggcagtc gttaatggcg agcattctct gttagtgctg gctggtgcag gaagcggaaa
     3001 aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga
     3061 gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg agcggattcg
     3121 cgaacggcta cataccgaag acattaccgc acgcacgttt catgcgttgg cgctgcatat
     3181 tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg ataccgctgc
     3241 ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc
     3301 gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag aaggtaactt
     3361 ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc ctcgatcgct gggtaagtct
     3421 gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg aagagattcg
     3481 cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc
     3541 gctgaaggca gaaaacgctg tcgatttttc gggccttatt catcaggcga ttgtgattct
     3601 ggagaaaggc cgctttatca gcccgtggaa gcatattctg gttgatgaat ttcaggatat
     3661 ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag caaaacagtc agacgacgtt
     3721 gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc aaatgtcgct
     3781 caccaccgct ttccatgaaa actttggtga aggcgatcgc tgtgatttag acacgactta
     3841 ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt attcagcaga acccaggcca
     3901 gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagccgtca cgttattgga
     3961 tgagagtcaa cttgacgctt tgctggataa gctctctggt tatgccaaac cggaagagcg
     4021 cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa aagcggcaac
     4081 acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag ggcaacaggc
     4141 ggattacgtc atcatcgttg gcttgcagga ggggagtggt ggttttccgg ctgcggcgcg
     4201 ggagtcgatt atggaagagg cgctactgcc accggttgag gatttcccgg acgctgaaga
     4261 acggcgttta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg cactgtttaa
     4321 caaagagaat ccctctccct ttgtggaaat actgaaaaat ctggatgtgc cggtggcgag
     4381 aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag acggtcagcg
     4441 agataacgct gataatcggg gataagaata tcgaccgcgt cgttgaaatg cggcgactgg
     4501 ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac cgtcgccaga
     4561 cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc ccagaagaat
     4621 atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc accccccatt
     4681 gggccactca acatcgcgtt gacgttcatg ccggtcgcgc gggaaatcaa gttaccggta
     4741 gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc cacccagctc
     4801 atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa
     4861 gtgcgagtcg tcagttccat aatgtacatc cgtagttaac tttcctacag attactgtaa
     4921 gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa aaatctcagg
     4981 aagatggttg tttttccgcc cattgcagga aagtatttcg cgtttgtggg tcagccagtt
     5041 taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga atagcatcca
     5101 gctcggaaac acccgaaagc agcgtactgt catctgccat catcgtggca aattgcggta
     5161 gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa tcaatcgttt
     5221 ttgccgttga ggtaatggcg agaatatcca gctttaccgg aatcggcgtc gcatcgccat
     5281 ccaacaattc aaggcgcggc gcggcatcaa aatggttagc ttcccgctca ttctccaggc
     5341 gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc accagcggtg
     5401 gggagatata cagccgttct tcactattgg aaagatgaat cgtcttctca acgcgaaaca
     5461 ctaactgatg cggtccattg tccagttcaa tgctgtccgc accgcgcagc agagagctgg
     5521 aaaccttctt gccgtcgagt accagaagat cgacatcggt tgaaagccgc agcgtggtgg
     5581 caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg gttttcatag
     5641 gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct ttaatcatac
     5701 ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt agctgcatgg
     5761 ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata atgaaagaaa
     5821 ccgatattgc aggcatttta acgtccactc acaccattgc gctggtgggg gcaagcgaca
     5881 aacccgatcg ccccagctat cgcgtgatga aatatctact cgaccagggg tatcacgtca
     5941 ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg tatggcacgc
     6001 tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt ccgtaattca gaagcggcgt
     6061 ggggcgtggc acaggaagct attgccattg gcgcgaaaac cctctggatg caactggggg
     6121 tgattaatga gcaagcggca gtactggcac gggatgccgg gttaaatgtg gtgatggatc
     6181 gctgcccggc tattgagatc cctcgcctgg gtctggccaa ataaaaaatc cccggaaggc
     6241 aaaaaccttc cggggatttg ttcagggatt agttacgcag acgcggggcc tggagttgtt
     6301 tgcggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca tcctgcaatt
     6361 cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta tcgtcctcca
     6421 tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt caccgccagc tcatcgggcg
     6481 acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga taacctaaca
     6541 gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc acctcccggg
     6601 aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg acgcgacagg
     6661 caaacccttt cagatatagc ccttccgggt aggtagcgat caccggatga tcggctgcct
     6721 gacggaactg ctctataaat tgtacatcac gaccggcatc aattgcggca tccgcgatga
     6781 ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg agaataccgc
     6841 cttcattcag caactgaatc gccagcatgt tgatgtcttt ataaccacga cacgcgccca
     6901 tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg tcaaattttt
     6961 caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca aactcagcct
     7021 tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagc gcttcctggg
     7081 aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg gcgaaaccac
     7141 cggtatagga gaaacagttc agcacacgtt tattttcaac gtagcggcgg gtagccaggc
     7201 ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc gtgctgaata tccaccagca
     7261 gcttcatgcc gtgttcttca atcggcagca gggcaggcgg caactcgccg gtgacgaggc
     7321 cctgggtcag ctccatccct tcttttttac gtaccgcaac atcgctgcga tcgtaaatcg
     7381 cacattccgg gtacagcgtt tgcagggcac taattaatgc cgcgcgctga tattctgcgc
     7441 cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta atacccggca
     7501 ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt tgcgccagcc
     7561 agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg tcgatagact
     7621 cagacgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa taagcgccgc
     7681 gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg ctggctttac
     7741 cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt aatgattttt
     7801 cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc gatgcaaaga
     7861 aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta aggataagga
     7921 gagcagcaat gtcgaaaatc tgcataattg cctggattta cgggcgagtt cagggcgtag
     7981 gatttcgcta caccacacag tacgaagcga aaagactggg cttaaccggg tacgccaaaa
     8041 atcttgacga tggcagcgtg gaagtggttg cctgcggtga agaagggcag gtagaaaaat
     8101 taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg gtgctcagcg
     8161 agccgcatca tccctcgggg gaattaaccg attttcgtat ccgttaaata cattttaccg
     8221 gttttggcag gccagcaatt ttggtggctt gcttagccgg gcctttcggg aacagtcggt
     8281 ataaatagcg gctgttacct ttttcttcac caaatttatt cgccatcgct tttaccagca
     8341 tacgaatcgc cggagacgta ttaaattcca ggtagaagtc acgtacaaaa cgcaccactt
     8401 cccagtgttc tggcgacagc gaaatccctt cgttctctgc aatcaccact gccagcggtt
     8461 cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc tctttacctt
     8521 cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa caaaaaacaa
     8581 agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg cggctggcga
     8641 agcccagaat gctcagtagg ctgacgaaga tgttgtacag cgaaacatac aggctaaccg
     8701 tggcacgaat atagttcgtt tcaccgccat gaatgatgtt gctggtttca aacaagatag
     8761 cgccagagga gatcagaatg aagaccgcgc tgatcgccag atgcagagca ggcagttgca
     8821 ggaagatatt agcaatcata ccaatcagta ccaccacaat acccgccatc agcataccgc
     8881 cgaggaacga catatctttg cgggtggtca gcacatatgc agagcagcag aagaacacta
     8941 acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac agataggtgt
     9001 tcagaattgg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg gagataatcc
     9061 cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata cccaccagag
     9121 tcagaatcag acccggagat ggcagcatca gcaccatact ggcagttgcg gtaatcgccg
     9181 aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta tgggtgctaa
     9241 gcagtgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca ctctctatga
     9301 cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga agttttaccc
     9361 atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtca ttaagcaaat
     9421 ataacgccct gagaattccg acaggcaaaa gaaaaagggg gtagcgttta gctaacccct
     9481 tatcttattt ggcggaagcg cagagattcg aactctggaa ccctttcggg tcgccggttt
     9541 tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca ctataaacat
     9601 cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc caaaagtgca
     9661 ttgatagcgt gaaataacag cagattgatc atttcatcac catgaattcc ttctctttta
     9721 ctcgtttagc aaccggctaa acatccccat cgcccggcta aagaaaaata ggtccatttt
     9781 tatcgctaaa agataaatcc acacagtttg tattgttttg tgcaaaagtt tcactacgct
     9841 ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa ggatgagctt tcgttttcag
     9901 catctcacgt gaagcgatgg tttgccttgc tacagggacg tcgcttgccg accataagcg
     9961 cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg atatgaataa cgaggaaaca
    10021 ttttaccagg ccatgcggcg tcagggcgtt acccggcgca gctttctcaa atattgtagt
    10081 ctggctgcca cgtcgctggg attaggcgcg ggaatggcac caaagattgc ctgggcgctg
    10141 gagaacaaac cgcgcattcc ggtggtatgg atccacggtc tggaatgtac ctgctgtacc
    10201 gaatctttta tccgctccgc tcacccactg gcgaaggacg tcatcctttc cctgatttcc
    10261 ctcgattacg acgataccct gatggctgcc gccggaaccc aggcggaaga agtctttgaa
    10321 gacatcatca cgcaatataa tggcaaatat atcctcgcag tagaaggtaa tccgccgctg
    10381 ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt ttattgagaa actcaaacgt
    10441 gccgctgccg gagccagcgc gattatcgcc tggggaacct gcgcgtcctg gggctgcgtg
    10501 caggccgcgc gacctaatcc gacacaggca acgcctatcg acaaagtcat caccgacaaa
    10561 cccattatca aagtacctgg ctgcccgccg atcccggatg tgatgagcgc catcattact
    10621 tacatggtga cctttgatcg cttgccagat gtcgacagaa tgggtcgtcc actgatgttt
    10681 tatggtcagc gtatccacga taaatgctat cgccgcgctc acttcgacgc cggagagttc
    10741 gtccagagtt gggatgatga cgctgcccgc aaaggttatt gcctgtacaa aatgggctgc
    10801 aaagggccta ccacctataa cgcctgttcc tccacacgct ggaatgatgg cgtttctttc
    10861 ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa atggtttctg ggatcgcggt
    10921 tcgttctaca gccgcgtggt cgatattccg caaatgggta ctcattccac cgccgatacc
    10981 gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg gtgtgcacgc agttgccagc
    11041 gccgttgacc aacgcagacg tcataaccag caacctacag aaaccgaaca tcagccaggc
    11101 aatgaggata aacaggcatg agcactcagt acgaaactca gggatacacc atcaataatg
    11161 ccggacgccg cctggtggtc gacccgatta cgcgcatcga aggccacatg cgctgtgaag
    11221 tgaatattaa cgatcagaat gtgatcacca atgccgtctc ctgcggcacc atgtttcgcg
    11281 ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc gtgggcgttc gttgaacgta
    11341 tctgcggcgt ctgtactggc gtacacgccc tggcttcggt ttacgccatc gaagatgcca
    11401 tcggtattaa agtgccggac aacgccaata tcatccgcaa cattatgctg gcaacgctct
    11461 ggtgccacga tcatctggtg cacttctatc agcttgccgg gatggactgg atcgacgtgt
    11521 tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact ggcgcaaagt ctctcctctt
    11581 ggccgaaatc atcccctggc tatttcttcg acgtacaaaa tcgcctgaag aaattcgttg
    11641 aaggcgggca gttggggatc ttccgcaatg gctactgggg gcacccgcag tacaaactgc
    11701 cgccagaagc caacctgatg ggctttgccc actatctcga agctctcgat ttccaacgtg
    11761 aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc gcatccaaac tggattgtcg
    11821 gtgggatgcc ttgcgccatt aacattgacg aaagcggcgc ggtcggggcg gtcaatatgg
    11881 aacgcctgaa cctggtgcag tcgattatca cccgcacggc agacttcatt aacaacgtga
    11941 tgatccccga cgccttagcc atcggtcagt tcaacaaacc gtggagcgaa atcggcacgg
    12001 gtctttctga taaatgtgtc ctcagctacg gcgcattccc ggatattgcc aacgactttg
    12061 gtgagaaaag tctgctgatg cctggcggcg cggtgattaa cggcgacttc aacaatgtgc
    12121 tgccagtgga tttggttgat ccgcagcagg tgcaggagtt tgtcgatcac gcctggtatc
    12181 gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg cattaccgac ccgtggtaca
    12241 accccggcga tgtcaaaggc agcgatacca acattcagca gctgaatgaa caggaacgct
    12301 actcgtggat caaagcgcca cgctggcgcg gtaacgcgat ggaagtgggg ccgctggcac
    12361 gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt tgagtcggtc gatcgcatga
    12421 tgtcggcgtt gaacctgccg ctttccggta tccagtcaac gttgggccgc attttgtgcc
    12481 gcgcgcacga agcgcagtgg gccgcaggta agttgcagta tttcttcgac aagctgatga
    12541 ccaacctgaa aaacggcaat ctcgccactg cttccactga aaaatgggaa cctgcaacct
    12601 ggccgacaga gtgccgtggc gtcggcttta ccgaagcgcc gcgcggagcg ttaggccact
    12661 gggccgccat tcgcgatggc aagattgatc tctaccagtg cgtggtgccg accacctgga
    12721 acgccagtcc gcgcgatccc aaagggcaga ttggcgctta tgaagcggcg ctgatgaaca
    12781 ccaaaatggc gatccccgag caaccgctgg agatactgcg tactctgcac agctttgatc
    12841 cgtgcctcgc ctgttcaact catgtgctgg gcgacgacgg tagcgagctg atctccgtgc
    12901 aggtgcgtta acagcgaagg agaatcatca tgcaacagaa aagcgacaac gttgtcagcc
    12961 actatgtctt tgaagcacca gttcgtatct ggcactggct gacggtgtta tgcatggcgg
    13021 tgttgatggt caccggttac tttatcggca agccgctacc ttccgtcagt ggcgaggcga
    13081 cgtatctgtt ctatatgggt tacatcaggt taattcactt cagcgccggg atgattttta
    13141 ccgtggtctt gctgatgcgg atctactggg cttttgttgg caatcgttac tcccgcgagc
    13201 tgtttatcgt gccggtatgg cgtaaaagct ggtggcaggg cgtgtggtat gaaatccgct
    13261 ggtatctgtt tctggcaaaa cgtccgagtg ccgatatagg ccataacccc atcgcccagg
    13321 cggcgatgtt cggctatttc ctgatgtcgg tctttatgat catcaccggt tttgcgctgt
    13381 acagcgaaca cagccagtac gctatttttg cgccgttccg ttatgtggtg gaatttttct
    13441 actggacggg tggcaactca atggacattc acagctggca tcggctgggg atgtggctga
    13501 ttggggcgtt tgtgatcggt catgtctaca tggcgctgcg tgaagacatc atgtccgacg
    13561 acacggtgat ctccaccatg gtcaacggct accgtagcca caaatttggc aaaataagta
    13621 acaaggagcg ttcatgagcg agcaacacgt ggtggtcatg gggctgggca acctgctgtg
    13681 ggccgatgaa ggcttcggcg tgcgggtggc ggaacggctg tatgcccatt accactggcc
    13741 cgagtatgtg gagattgtcg atggcggtac tcagggactg aacttgctgg ggtatgtcga
    13801 aagcgccagc catctgttga ttctcgatgc cattgactac gggctggaac ctggaacgct
    13861 gcgaacctat gccggagaac gcattccggc ctatctcagc gcgaagaaaa tgagcctgca
    13921 tcagaacagt ttctccgaag tgctggcgct ggcagatatc cgcggacatc tgccagcaca
    13981 tattgccctc gtcgggctgc aacccgcgat gctcgacgac tacggcggca gcctgagcga
    14041 actggcacgg gagcaactgc ccgctgcgga acaatcggcg ctggcgcagt tggctgcgtg
    14101 gggaagtgtg ccgcaaccgg ctaatgaatc gcgttgtctc aattatgact gtctgtcgat
    14161 ggaaaattac gaaggcgttc gcttgcgcca gtaccggatg acacaggagg agcagggatg
    14221 agcaacgaca cgccatttga tgcgttgtgg caacgaatgc tggcgcgcgg ctggacgccg
    14281 gtcagtgaat cccgtcttga cgactggctt acgcaagcgc cagacggcgt ggtgttatta
    14341 agcagtgacc cgaaacgcac gccagaggtc agcgataatc cggtaatgat tggcgaatta
    14401 ctgcacgagt ttcccgacta tacatggcag gtggcgattg ctgaccttga gcagagcgaa
    14461 gccatcggcg atcgctttgg cgcctttcgc tttcctgcca ctttagtgtt taccggcgga
    14521 aactatcgcg gcgtgctgaa tggtattcac ccgtgggcgg aactgataaa cctgatgcgc
    14581 gggcttgtcg aaccgcagca ggagcgtgcc tcatgagcga aacttttttc catctgctgg
    14641 ggccaggaac gcaaccgaac gatgacagtt tcagcatgaa tccactgccg atcacctgtc
    14701 aggtgaatga tgaaccgagt atggcggcct tggagcaatg tgctcacagc ccgcaggtga
    14761 ttgcgctgtt aaacgagtta caacatcaac taagcgaacg ccagccgccg ttgggcgagg
    14821 tgctggcagt cgatctgtta aatctcaacg ccgacgatcg tcactttatc aatacgcttc
    14881 tcggggaagg ggaagtgtca gtgcgcattc agcaggctga cgacagtaaa agtgaaatac
    14941 aggaggcgat cttctgcgga ttatggcggg tgcgcagacg tcgcggcgaa aagttgctgg
    15001 aggacaaact ggaggctggc tgcgcgccgc tggcgttgtg gcaggcggca acgcaaaatc
    15061 tcttgccgac agattcgctg ttaccgccgc ccattgatgg cctgatgaat ggcctaccgt
    15121 tggcgcatga gttactggca catgtacgta accccgacgc gcagccgcac agcattaatc
    15181 tgacgcaatt acccatcagc gaggctgatc ggctttttct ctcgcgtctc tgtgggccgg
    15241 gaaatattca gattcgtacc attggctatg gcgagagcta tatcaacgcc acggggttac
    15301 gccatgtctg gcatttacgc tgtacggaca ccttaaaagg cccgttactg gaaagttatg
    15361 aaatctgccc aataccggaa gtggtgctgg cagcgccaga agatttggtc gactctgcgc
    15421 agcggcttag cgaggtatgt cagtggctgg cggaagctgc accgacgtaa aaagacggta
    15481 agtatcgctt tcagtcttat gaatatcgca atcggcgaat acctctggtc gtagagtttc
    15541 aggataaaga gggagatcta acattatcgg gttatttttc tctcttcgcc tacaggagtg
    15601 cgcatgtggg atgtcattga tttatcgcgc tggcagtttg ctctgaccgc gctgtatcac
    15661 tttttatttg taccccttac cctggggctg atttttttgc tggctatcat ggaaaccatt
    15721 tacgtggtta ccggcaaaac aatctaccgc gatatgacgc gcttctgggg taagctcttc
    15781 ggtatcaatt ttgctcttgg cgtggctacc ggcctgacca tggagtttca gtttggtact
    15841 aactggtcat tctattccaa ctatgtgggc gatatttttg gcgcaccact ggcgatggaa
    15901 gcattaatgg ccttcttcct cgaatccacc tttgtcgggc tgttcttctt cggctggcaa
    15961 cggctgaata aataccagca cctgctggtg acgtggctgg tggcgttcgg ttcaaatctc
    16021 tctgcattgt ggatattgaa tgccaacggt tggatgcaat acccgaccgg tgcgcatttt
    16081 gatatcgaca ccctgcgtat ggagatgacc agcttcagcg agctggtctt taacccggtc
    16141 agccaggtga aatttgtgca caccgtcatg gcgggctacg tgaccggggc catgtttatt
    16201 atggcgatca gcgcctggta tttactgcgc ggacgggagc gcgatgtcgc attacgctcg
    16261 tttgccatcg gttccgtctt cggtactctg gcgattatcg gtaccctgca actcggagac
    16321 agttctgcgt atgaagtcgc gcaagtacaa ccggtaaaac tggcggcgat ggaaggggag
    16381 tggcaaacgg aacctgctcc tgcaccgttc catgtggttg cctggccgga acaggatcaa
    16441 gagcgtaacg cctttgccat caaaattccc gcgctgctag ggatcctcgc cactcactca
    16501 ttagataaac ccgtgccggg tctgaagaat ttgatggctg aaacctaccc acgcttgcaa
    16561 cgcggacgta tggcctggct gttaatgcag gaaatatctc aaggcaatcg tgagccgcat
    16621 gtgttgcagg cattccggga gctggaaggc gaccttggtt acggcatgtt gctctcccgc
    16681 tatgcgccgg atatgaatca tgtcacagcc gcacagtacc aggcggcgat gcgtggcgcg
    16741 atacctcagg ttgcgccggt attctggagt ttccgcatca tggtgggctg tggttccctg
    16801 ctgctactgg tgatgctgat tgcgcttgtc cagacgctgc gtggcaaaat cgaccagcat
    16861 cgctgggtgc tgaaaatggc gctctggagt ttgccgttgc cgtggattgc gattgaagcc
    16921 gggtggttta tgaccgagtt tggtcgtcag ccgtgggcga tacaggacat cttaccgaca
    16981 tactccgcgc actccgcttt aaccacagga caactggctt tctcactgat catgatcgta
    17041 gggctttaca ccctgttctt aatcgccgaa gtctacctga tgcagaaata tgcccgtctg
    17101 gggccgagcg cgatgcagag tgaacaaccg acgcagcaac aggggtaaag gagaaaatca
    17161 tgtttgatta tgaaacattg cgcttcatct ggtggctgct gattggcgtg atcctggtgg
    17221 tctttatgat ctccgacgga tttgacatgg ggatcggctg tttgctgccg ctggtggcgc
    17281 gtaatgatga tgaacgccgg atagtgatta atagcgttgg tgcgcactgg gaaggcaatc
    17341 aggtctggtt gatcctcgca ggtggggcat tatttgcagc ctggcccaga gtgtatgcag
    17401 cggcgttttc cggcttttat gtggcgatga tcctggtgct gtgctcgctg ttcttccgtc
    17461 cgctggcctt tgattatcgc ggaaaaattg ccgatgcacg ctggcgtaaa atgtgggatg
    17521 ccggtctggt catcggcagt ctggtgccgc cggtagtctt cggtatcgcc ttcggcaact
    17581 tgttgctcgg cgtgccgttt gccttcacac cgcaattacg cgtggagtat ctcggcagct
    17641 tctggcaact gctgacgcca ttccctttat tgtgtggttt gctcagcctt gggatggtga
    17701 ttttgcaagg tggcgtctgg ttacaactga aaactgttgg tgtgattcat ctgcgttcac
    17761 agctggcgac taaacgcgct gcactgttgg tgatgctgtg ctttttgctg gcaggttact
    17821 ggctgtgggt cggtattgat ggctttgtac tgctcgccca ggatgctaac ggtccttccc
    17881 atccgttaat gaaactggtg gcagtgctac ctggtgcctg gatgaataat ttcgtcgaat
    17941 cgcccgtttt gtggatcttc ccgctgctgg ggttcttctg cccattgctg acggtgatgg
    18001 cgatttatcg tggtcgcccg ggttggggat ttttgatggc atcattgatg caatttggcg
    18061 tgattttcac ggcaggcatc acgctgttcc cctttgtcat gccgtcaagt gtgagtccaa
    18121 tctccagcct gacgttgtgg gacagcactt ccagtcagct gacgctgagc attatgttgg
    18181 taatcgtgct gatatttttg cccattgtgt tgctctacac tctctggagc tactacaaaa
    18241 tgtggggacg catgacaaca gaaactctcc gccgtaacaa aaacgagttg tactaaggag
    18301 caggaacatg tggtatttac tttggttcgt cggcattttg ttgatgtgtt cgctctccac
    18361 ccttgtgttg gtatggctgg acccgcgtct gaaaagttaa cgaacgtagg cctgatgcgg
    18421 cgcattagca tcgcatcagg caatcaataa tgttagatat gaaaagcgga aacatatcga
    18481 tgaaagcgat cttaatccca tttttatctc ttctgattcc gttaaccccg caatctgcat
    18541 tcgctcagag tgagccggag ctgaagctgg aaagtatggt gattgtcagt cgtcatggtg
    18601 tgcgtgctcc aaccaaggcc acgcaactga tgcaggatgt caccccagac gcatggccaa
    18661 cctggccggt aaaactgggt tggctgacac cgcgcggtgg tgagctaatc gcctatctcg
    18721 gacattacca acgccagcgt ctggtagccg acggattgct ggcgaaaaag ggctgcccgc
    18781 agtctgctca ggtcgcgatt attgctgatg tcgacgagcg tacccgtaaa acaggcgaag
    18841 ccttcgccgc cgggctggca cctgactgtg caataaccgt acatacccag gcagatacgt
    18901 ccagtcccga tccgttattt aatcctctaa aaactggtgt ttgccaactg gataacgcga
    18961 acgtgactga cgcgatcctc tgcagggcag gagggtcaat tgctgacttt accgggcatc
    19021 ggcaaacggt gtttcgcgaa ctggaacggg tgcttaattt cccgcaatca aacttgtgcc
    19081 ttaaccgtga gaaacaggac gaaagctgtt cattaacgca ggcattacca tcggaactca
    19141 aggtgagcgc cgacaatgtc tcattaaccg gtgcggtaag cctcgcatca atgctgacgg
    19201 agatatttct cctgcaacag gcacaaggaa tgccggagcc ggggtgggga aggatcaccg
    19261 attcacacca gtggaacacc ttgctaagtt tgcataacgc gccattttat ttactacaac
    19321 gcacgccgga ggttgcccgc agtcgcgcca ccccgttatt ggatttgatc atggcagcgt
    19381 tgacgcccca tccaccgcaa aaacaggcgt atggtgtgac attacccact tcagtgctgt
    19441 ttattgccgg acacgatact aatctggcaa atctcggcgg cgcactggag ctcaactgga
    19501 cgcttccagg tcagccggat aacacgccgc caggtggtga actggtgttt gaacgctggc
    19561 gtcggctaag cgataacagc cagtggattc aggtttcgct ggtcttccag actttacagc
    19621 agatgcgtga taaaacgccg ctgtcattaa atacgccgcc cggagaggtg aaactgaccc
    19681 tggcaggatg tgaagagcga aatgcgcagg gcatgtgttc gttggcaggt tttacgcaaa
    19741 tcgtgaatga agcacgcata ccggcgtgca gtttgtaatg cataaaaaag agcattcagt
    19801 tacctgaatg ctctgaggct gatgacaaac gaagaactgt ctaatgcgta gaccggaaaa
    19861 ggcgttcacg ccgcatccgg tcactttcag ttttactctt tctcggagta actataaccg
    19921 taatagttat agccgtaact gtaagcggtg ctggcgcgtt taatcacacc attgaggatt
    19981 gcgcctttaa tattaacgcc agcctgttcc agacgctgca ttgacaagct cacctctttg
    20041 gcggtgttca agccaaaacg cgcaaccagc aggctggtgc cgacagagcg tcccacgacc
    20101 gcagcattgc tcaccgccag catcggcggc gtatcgacaa tcaccagatc gtaatggtcg
    20161 ttcgcccatt ccagtaattg acgcatccga tcgcgcatca gcagttcaga cgggttaggt
    20221 ggcacctgac cgcgagtaat cacatcaaag tctcctttgc caaaatgctg gatcactttg
    20281 ttgagctcat ctttacctgc cagatattcc gacaagccat gttcattact cacggtaaac
    20341 aggttatgcg aataaccacg gcgtaagtcg gcatcaataa ataacacttt ttgatcggac
    20401 tgggcgatca ccgctgccag agttgaactg acaaacgttt taccactgtc tggcgtcgca
    20461 ccggtgatca tcagaatgtt attctcagtc tccatcatag cgaaatgcag actggttcgt
    20521 agcgcacgta cggcttccac agcagaatcc gccgggttat ccaccgccag gaaggggata
    20581 tttttagtac gatggcgctg ctgattagaa aataaatttt tcttacgcag acgggtgcgt
    20641 ttatccagcc actcggacat tgggatagtg gaataaacgc tgatgccgtg ctcttccagt
    20701 tgttccgggg cttctacacc acgacgcaac atcgcacgcg ccagcacggc actcaccgaa
    20761 ataaacaggc caagaataaa accaagcacc acattcaacg cttttttcgg tttcactggc
    20821 tgcggctgag tgactgccgg gtcgataatc cgcacgttac caatggcact ggatttcgaa
    20881 atactcaact cctgctggcg gttaagtaat tgcagatata ccgcacggcc cgcttctacg
    20941 tcacgactta aacgcaacac ttcctgttgg gtggaaggca ttgccgatac ccgcttattc
    21001 aggcgtttgc gttcttgctc cagcgtctgg cgtttttcca gcagcgcacg ataagttggg
    21061 tgatctttct tatacagctg ggagatctct gcctcgcgga aggtcagctc attgagttga
    21121 ttatcaacgt tcacaatctg ctcaagaacg gctttggctt ccaggttaag gtcaaccgaa
    21181 tcgcgctgct ggcgataaac gttgagtttt tcttccgcct ggtccagctc gctgcgcact
    21241 tcaggtaact ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc ctggcgagcg
    21301 atattctgtt gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat caactgggga
    21361 tcatcaccag tcatggtaag ttccagcatc ccgctttctt tactgcgttc gctaacggta
    21421 aaggtttcct gcaatgcgtt aatcgcttcc agttcggtac gctggctcag gacaaactgt
    21481 gttcctggtt tggccttaat gtccgcgata gtcagcgcaa cgccatcttt ttccagacgc
    21541 tggccgacca taccattgac ggtgaactct tcaccttcca gtgtatagtg gccgttttcc
    21601 ccaaccgtga gtgtcagttg ctgatcctga ccattcagtt gtggaatatg catccagctg
    21661 atcgccagct cacctagttt ttctttggtt aatctcgccc agccgcgacc cacaatcgga
    21721 aaatacttct gctcaactat gtcgcgcaga ttcagttcag caatggtttt accgagaatc
    21781 atgcgcgatt gcagcagttg gatctccggt gcagactcgg gcgatgagtt agggatcata
    21841 tcgctcaggc cgctgagaat ggcgttgccc tgtttttgct caacctggac cagagtatct
    21901 gcctgataaa ttggtgtgct taacagcgat cagcgtgaat aacgcggtca cgctgataat
    21961 aaacttacgg tgatcccata actcgccgac cagacgaagc agatcgatct cattttcctg
    22021 agtgctgcct ggtggcgtat tcatattttt agttgtcatc gtattttata aactcaaatt
    22081 accggctgag gcgcttcgcc cattcctgac tggcgcgctc caacatactg tagacatgtt
    22141 caaatgcgtc ctgactttta cgatagggat ccgggatctc tttctgttcc agccattgcc
    22201 caaacagcat tgtcttcccg cggacctcgg gagcaattgc ggtaacctga gcaatatgtt
    22261 ccgactccat tgccagaatc agatcgtaat ttcgtgccat ctcagcagtg agcttacgtc
    22321 cggcatgtcc ttcaagagat acgccatgat tagcggcaac atctgccgct gtcgcatctg
    22381 cagggtgttt taccagacca tgaacacctg ccgatttgac ttttacgccc ggcagacgtt
    22441 tacgcagcaa gcgctcgcca atgggcgaac ggcaaatgtt gccggtacaa accactagga
    22501 ttgagttaaa ttttagttgg gccatgtttg aatccaccgt gaagtctctg tcaggtcatg
    22561 caccccggaa atcgtcggta ccagcagaga aatcacacgg ttccaacgag ccagcggcgc
    22621 ggtagtgaca taaacaatgt cgtaaggttc aagctggaat tccgttccga gtatcattgc
    22681 cgatgcgtcc ttcgcattca gctgataaat attggcgatt ttgccattct gcttgttttg
    22741 cgttgcacga ataacaaaaa tccccgtcgc atcagcgaca tcctgattca taccctcagc
    22801 gttacccagc gcttctgcca gggtcatacc gctgcggtcc attttcagcg tgctctgttt
    22861 acccacttca cccatcacga agactttcag atcatcgttg cgcggaataa ataaaatatc
    22921 gcccgggtgc agcagtttgt tctggcgcaa gtcgccacgc tgcatcagcg catagaggtt
    22981 cactttggtc tttacgccat tctgggtgag caccacgttg cgccagtctg catctgcggt
    23041 cagaccgccc gcggcgttaa tcgcatccat gatggtcaga ggaatattgg taatcggctg
    23101 ctgacccgat ttgctgactt cgccggtgac gtacgctttt tgcgatcgga acgccgcgac
    23161 gctgacatcg acttgcgggc tttcaatgac tgaatcgaga cgcgctgtaa tctcgttgcg
    23221 tacctgggta agtgttttcc cggccacttt caggcgacca atataggggt agaaaatcgc
    23281 accatcggcg ttaacccagt tgccggtatc actggcgcta cggtattgcc ctgcgggggt
    23341 ggttagttcc gggtgatccc agaccgtaac catcaacaca tcaccaatcc cgatacgata
    23401 ttcatagttt gcgatttgct gctccagctc cgggttagcc tgcgacataa tcggtttggc
    23461 gcgcagctga tcaatcaatc ctggcgtgac gggatagata ttgaccattt tgtccaggtc
    23521 atactgattg tcaggtagtt caatgacatc cttgttactg gtcgataaat tctgaccagg
    23581 aacaagggta catgctgtca aagacagaac agccaatgtc agtaccgaaa acttaaaaat
    23641 gttctttttc attggtttat atttatcagt caatgtaatg ttatgaatta cgtcaggtta
    23701 ttgtccaacc ggtacttctc gctctgccgt cattggatcg agctggtatt tgcgtgacag
    23761 catttgtcca ccatcacgcg tcaatggtgt ccacgaaacc accgcgcggt tgcggttagg
    23821 tccaatggtc atcaagtcga acggaatcga gatataaaaa cctttactaa agccgccttc
    23881 gccgtagtca tctttagata cgttactgat tgccgcccat acccctaccg ccacgccgct
    23941 gtcaaagagt ttggcgacgt cgatcgttgc gcctttatct tttgccagat attgcccaac
    24001 gctaagtttc atcagtacgc cattgagcgt cggcgggttc cagtaagccg tcacgaaacc
    24061 tgtgggcgtg gaataatcgg tgaagcgcat catgttgtcc cagtcacgct gcttcacgta
    24121 gttaacgtcc acacccagcg cccagctggc atctagcggg cgataaagaa gctcggaacc
    24181 gacaccggca tacatcgttt ccagataacc gccatacacc tggccataga aaccattgcc
    24241 taagtcggca aagtaattcg cttgcaagtt gttgagataa acgtcattgc gaacgtaatc
    24301 acggatatgc gtgcgcacgc ggggcagggt agagtccgcg ggtaacagtg aagacttaaa
    24361 cttgtcgtag ttgttgtaaa tattggtgaa aataccgtca tcaagcagca ggtggtcggt
    24421 aaaccagtag cgggcgctgg acatcagccc cagctggaac atatagaaat cttccgggcc
    24481 gcccagcgac tgtgaaagtg ttgggttgaa agagtagcta aagcgatctt cacgaatacg
    24541 gtagcaccga ccaaaggcag aaagatcttc tgcttcaaca cgttgttgtt gcagttgctc
    24601 tgatttacca ggcgctgtgc cggccagctg tttgcgcagg ctggctacgt cggtttcggt
    24661 agtcaccatc gccatatgct cgcgcttttg cgtcacgcta atcttctcaa cgccttgcgg
    24721 caggttattc accagaatcc ggttggcacg atcgaccgct tcgcgagagt cacggtattt
    24781 gtattgctga ccagacatat acagcgtctt atcgcgtagc tgaatttctg gcgcgtcaaa
    24841 gcccgcgtta tacttcaggg cggtaagttg gtttgctacc gtggtgtact gcaaaccttc
    24901 agactctggc gcaggctgcc aggccggttt tggcgtatcg cgtagggcag ggcgcagatc
    24961 gttgaaattg gtccgtaaag tgaaaccaaa catcaacgtg tttccgcgtt cataactgag
    25021 gttgaggtct gcccagctgg cagcgcgata aactgcaccg acgttgaaat ggcttgcctg
    25081 aggcagtttg ccagcgaaat cattctggta attgttgccg tcgtattcga gtttcagacg
    25141 caggggattc cacggcgttt gatactcaat gccgccaaag atggaagccg ggccacgaaa
    25201 gatatcgcta aagctgatat cgcccgcatc gtgagactct gcgcgatgac aatatttatc
    25261 gctcacccgg caaaacgggt tggtaatatt gcccgcatta ccggcgtagc cccatgccat
    25321 cccgagggtg aaatcaaatg gccccgcttg cttgctggcc accagatatt caccgtcaaa
    25381 caaacccgta ccagcaatat cacgtttacc aaacgccact tgcggtagcc agtaaccttc
    25441 ttcccacagg cgaagtttaa aatcgaatga tttgtctttg tatgactgat cgccgctgaa
    25501 atcttcccac tggctatatt tgcgtgtgcg cacatccgta taacgaatgg tgccttccag
    25561 ccatgggaac agcgccacgg aggtggagta gaaccggtat tgatcgttat cccggtagtt
    25621 gacgctgaat tcaccttccg gtgcgatgcg cgcatttggc atctgcaaca atcctgtgcc
    25681 gccaaagtct gattgcgacg aacccagcgg atccgggtag gttaatactt cagcatgaca
    25741 ggcactggag acggcaatgg ccaggcagct taaaagaaat ggccaggcag cttaaaagat
    25801 aagaattctt cttcatcagt caggaacacg ttgcgttaaa acagacacaa tctggtcatt
    25861 taaatcagca tatttttcgg gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc
    25921 aggcggtggt tccacatgtc ttttattcca cagggcaaca ggggcgacga cagtctcacc
    25981 ctcaggcgta atcaccataa cgttattctt gtctgcgcct gcaaggcggg ggtgatcttg
    26041 cagataatcg gtaacgctac ggcctgcctg ccagggcaat tgcccagccc cagaaaccgc
    26101 acccagcaga gtaatagtca ccggacgctg tacggtatag agcgtatagt ccccgaccaa
    26161 cgggggattg ctgttctcat ccactcgtac aaaatcaggg tccagtttga ccggtaagcg
    26221 accagtgata ttgagattga gtaactgctg gcgcacggat ttaatcgttg ccgccacatc
    26281 gtcgtcagct tctgcttccc aggaagcaag ctgtgccatg acatgttggt agtctttaag
    26341 cgctttagct ttcgccgcag agtcggtcag caaggcacca ggccaccaga ggcgatcgcg
    26401 cagttgcggt tgtgtcacca actggacaac attttccacg gggccaacgg aaagtgtctg
    26461 ttgttcgcta ggcagataaa tagtcaccgt tccttgcgca aaggcatggg gtgtcattac
    26521 gtaaagtacg ctggcaatga aatacgactg taatttattc atggtgcggg tttaagaaac
    26581 gtcatttcta cgggaataac ccctgcgcct aacatttgcc gactttgacg aacctggcca
    26641 gtagccgaat cgacccagaa tgtgttttgc cactgttttt ctggcagcgt ggaagtcacc
    26701 tcttcctgcc agacattaca cagcacattg tgacccgcaa tattcagcgt ctctaatcca
    26761 gcaaaggaaa aagtagaact tagggtcgca gagcgaaaat ggttgtcttc actccagaga
    26821 atatcgcgcg tccagcggct gccatctttt attgccagcg cgttacgcag aggggcccgc
    26881 ccgggatttg tcacttccag cagattatta ttaagtttga cggtctttaa aagacgtcca
    26941 ttgtgggtaa ccagcatggc gttatcctgg gataaccatt tgctttgttc ttgttctata
    27001 tatcccagta ccacaaagat tcgttgccct tcattaaggc gtaaatacat cgtggaataa
    27061 ggcaacgcct ggatctgctg agccgctaca gtgatatcct gattatcgaa aaggctggcg
    27121 cgaaatgtat cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa
    27181 atcgataaaa taagagggcg cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat
    27241 ctctaaagcc cggtacgaat atcgcaaccg ggtattattt aacttgaatt tcgacgcgtt
    27301 taagcagaat tagcgcgtag tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc
    27361 actgtcattt gctgcaacta ctgccaacgt cgccacacca acagccgttg ccacaccgac
    27421 aactgtcgcc gtgccgacag cgccagcagc cacgccacta gtggtagccg cagtcgtggt
    27481 tgcacctgta gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg tcccgtttgc
    27541 tgcttcaggg gcagcaaacg cagctggcgt cgtcaacatg aaggccataa ggattgcaga
    27601 aagcttgtgt ttcataactt ttcctttatt catcgcatgg acaatacgta cgcttgagat
    27661 caggattgtc tactttgagt ataacatcct tgtgaaatct ccctttcctc ataccgctag
    27721 cactatatac tgcgggtggg aaaggcgcgc agagggaaat ataagattgt taacgatttc
    27781 cctctggaaa tacgacttgt gagcataaga taaatcttag aaaaatacga attaaagata
    27841 agaaaattgt tacgaaagct attaattatt gttggtaata tagtttcaag tggtactata
    27901 actggctgtt aaatggcatt tataactatt aggtgcagag atattcgctt aaatggatta
    27961 gtttcttagt ggaaaagtaa ctgatgttat aatgtatggt gttgactatt gaactattcc
    28021 aataaaacca gtcagcttta aacaagcagc gtcatattaa gagagataaa catttgccgc
    28081 tgttggtcct cgcaggccat ttacgcggca aaattccacg cgtaatcctg gtataagcac
    28141 ttctgcgtcg cggggagtga atgcggaaat atggacctgg acttctttac gaccgtcgga
    28201 ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt
    28261 acgggacaaa caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat
    28321 aaagccagcg atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta
    28381 tcatgcctta atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa
    28441 gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagtg
    28501 gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct
    28561 aataa
//
LOCUS       DAJTRN010000050        28394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRN010000050 DAJTRN010000000
VERSION     DAJTRN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..130)
                     /gene="yjdI"
                     /locus_tag="OO328_002206"
     CDS             complement(<1..130)
                     /gene="yjdI"
                     /locus_tag="OO328_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5930760.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNL"
     gene            311..1942
                     /gene="dcuS"
                     /locus_tag="OO328_002207"
     CDS             311..1942
                     /gene="dcuS"
                     /locus_tag="OO328_002207"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5930761.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1939..2658
                     /gene="dcuR"
                     /locus_tag="OO328_002208"
     CDS             1939..2658
                     /gene="dcuR"
                     /locus_tag="OO328_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5930762.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3229..4569
                     /gene="dcuB"
                     /locus_tag="OO328_002209"
     CDS             3229..4569
                     /gene="dcuB"
                     /locus_tag="OO328_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5930763.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4647..6293
                     /gene="fumB"
                     /locus_tag="OO328_002210"
     CDS             4647..6293
                     /gene="fumB"
                     /locus_tag="OO328_002210"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5930764.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6416..7045
                     /locus_tag="OO328_002211"
     CDS             6416..7045
                     /locus_tag="OO328_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5930765.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7184..8605)
                     /gene="melB"
                     /locus_tag="OO328_002212"
     CDS             complement(7184..8605)
                     /gene="melB"
                     /locus_tag="OO328_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5930766.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8708..10063)
                     /gene="melA"
                     /locus_tag="OO328_002213"
     CDS             complement(8708..10063)
                     /gene="melA"
                     /locus_tag="OO328_002213"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5930767.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10346..11254
                     /gene="melR"
                     /locus_tag="OO328_002214"
     CDS             10346..11254
                     /gene="melR"
                     /locus_tag="OO328_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5930768.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11453..13720
                     /gene="adiA"
                     /locus_tag="OO328_002215"
     CDS             11453..13720
                     /gene="adiA"
                     /locus_tag="OO328_002215"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5930769.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14045..14806
                     /gene="adiY"
                     /locus_tag="OO328_002216"
     CDS             14045..14806
                     /gene="adiY"
                     /locus_tag="OO328_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5930770.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14943..16280
                     /gene="adiC"
                     /locus_tag="OO328_002217"
     CDS             14943..16280
                     /gene="adiC"
                     /locus_tag="OO328_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5930771.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16384..18027
                     /gene="eptA"
                     /locus_tag="OO328_002218"
     CDS             16384..18027
                     /gene="eptA"
                     /locus_tag="OO328_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5930772.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18024..18692
                     /gene="basR"
                     /locus_tag="OO328_002219"
     CDS             18024..18692
                     /gene="basR"
                     /locus_tag="OO328_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5930773.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18693..19793
                     /gene="pmrB"
                     /locus_tag="OO328_002220"
     CDS             18693..19793
                     /gene="pmrB"
                     /locus_tag="OO328_002220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5930774.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19769..19858)
                     /gene="pmrR"
                     /locus_tag="OO328_002221"
     CDS             complement(19769..19858)
                     /gene="pmrR"
                     /locus_tag="OO328_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5930775.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19970..21472)
                     /gene="proP"
                     /locus_tag="OO328_002222"
     CDS             complement(19970..21472)
                     /gene="proP"
                     /locus_tag="OO328_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5930776.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21736..22614)
                     /locus_tag="OO328_002223"
     CDS             complement(21736..22614)
                     /locus_tag="OO328_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5930777.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFDDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22611..24839)
                     /gene="crfC"
                     /locus_tag="OO328_002224"
     CDS             complement(22611..24839)
                     /gene="crfC"
                     /locus_tag="OO328_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5930778.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25242..25577
                     /locus_tag="OO328_002225"
     CDS             25242..25577
                     /locus_tag="OO328_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5930779.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25735..26178
                     /gene="yjdN"
                     /locus_tag="OO328_002226"
     CDS             25735..26178
                     /gene="yjdN"
                     /locus_tag="OO328_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5930780.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26311..27099
                     /gene="phnC"
                     /locus_tag="OO328_002227"
     CDS             26311..27099
                     /gene="phnC"
                     /locus_tag="OO328_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5930781.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27124..28140
                     /gene="phnD"
                     /locus_tag="OO328_002228"
     CDS             27124..28140
                     /gene="phnD"
                     /locus_tag="OO328_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5930782.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28246..>28394
                     /locus_tag="OO328_002229"
     CDS             28246..>28394
                     /locus_tag="OO328_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5930783.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7295 a         7415 c         6933 g         6751 t
ORIGIN      
        1 tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga catatcgcag
       61 tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg tccagtagcg
      121 cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca tagccccgga
      181 atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata tagccaatca
      241 tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa tttgtcacac
      301 aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc cgatgaaatt
      361 gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat tggtggtgca
      421 tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca acaaggcact
      481 ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc agaaaaaacc
      541 gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg atctgctgtt
      601 tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc agcgtattgg
      661 tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa atgtcgctat
      721 caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg atgaaaatca
      781 taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc aacagatcaa
      841 tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg gactgattgg
      901 cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac cctacgaaat
      961 ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag gcgtcgttgc
     1021 cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat tgctgaatta
     1081 ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt cacaggtggt
     1141 agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga ttacgattaa
     1201 agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta tcatcggtgc
     1261 catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac tcgacggtct
     1321 ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata aattgcatgt
     1381 gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca ttctcaaaac
     1441 agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat ctccggttat
     1501 cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata cgctgatttt
     1561 aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga cgctgattac
     1621 cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac ccggtggcga
     1681 aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta acgatgatgg
     1741 accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct cgacaaaagg
     1801 aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc tcggcggcag
     1861 catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga taccctggga
     1921 cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc aatggtcgcg
     1981 gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg aacagcctcg
     2041 acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga cctgatattg
     2101 ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct gcataacgcg
     2161 cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac cattaaagat
     2221 tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc ccgctttgaa
     2281 gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca gtattatgac
     2341 caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga tcctcgtcgc
     2401 ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat tgacgcgcat
     2461 caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc gcgtgtttcc
     2521 tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag tatccattat
     2581 ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta ctcactgctg
     2641 aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat taccgtaatg
     2701 caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca gatcaaatat
     2761 aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt gtttttaatt
     2821 tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg tatttttacc
     2881 cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg agaacaaaaa
     2941 atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct gcgcagtaca
     3001 aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat cacagtgcat
     3061 aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta aagccggtaa
     3121 cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag aacgtgactg
     3181 tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat gttatttact
     3241 atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg tggtatcgcg
     3301 ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca ccttcagcca
     3361 ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc atcggcgacc
     3421 ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct gctgcgccgc
     3481 aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac cattctttgc
     3541 ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc cattaagaac
     3601 aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat ggggattatc
     3661 gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa tgtcaccttt
     3721 gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc gacgttaatc
     3781 ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga taaagacgaa
     3841 gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg tgataccgcg
     3901 acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat tttcctcggg
     3961 gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc cttcggcggc
     4021 aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg ggcgctgatt
     4081 attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt ccgttccggt
     4141 atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat gttcggtgcg
     4201 catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc gtgggcgtat
     4261 gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc gctggcggcg
     4321 attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc ttcagcaccg
     4381 gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc gattcagttt
     4441 gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt tattctgccg
     4501 gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc gatgtacggg
     4561 ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt ttcgaataac
     4621 aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac caggcacctt
     4681 tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac gttagcgttg
     4741 ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc ctgctggcgc
     4801 agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa caggttgcgg
     4861 ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg caattcttaa
     4921 gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc ggcaccgcga
     4981 tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa gaagcgctgt
     5041 cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa aacgcggcgc
     5101 tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc gacctgtacg
     5161 cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc tctgccaaca
     5221 aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg aaaaacttcc
     5281 tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat atcgcgtttg
     5341 tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca agcgctcact
     5401 attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc gatgtccagc
     5461 tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag tttggcggta
     5521 aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca tcctgcccgg
     5581 taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc aaccgcgaag
     5641 gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa gaactgcgtc
     5701 aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa gagatcctcg
     5761 cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc attattgtgg
     5821 gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa gaacttccgc
     5881 agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct gccggttatc
     5941 catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg gatctgctgc
     6001 aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag caggttaccg
     6061 acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg gcggcggtac
     6121 tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg ggtatggaag
     6181 ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat gacaaaggta
     6241 acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag taacctcttc
     6301 ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct tttgcgattg
     6361 taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac tcaccatgac
     6421 ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt taatcctgat
     6481 ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga caccggtgat
     6541 tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc tgctgctcta
     6601 tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca cctacgcgaa
     6661 agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc cgtatgacaa
     6721 gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa ttctcgttcg
     6781 cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg tggcgctggc
     6841 gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg gccagggagc
     6901 ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata tgttctgcgc
     6961 gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc gccagctacg
     7021 gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga tgcggcgtga
     7081 attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga cgtggcctga
     7141 taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct ttcacatcac
     7201 tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg cgatatttat
     7261 ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag aaacggaagt
     7321 agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata aactgcatac
     7381 ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc cctaacacaa
     7441 ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc tgcacggagt
     7501 aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg gtatctgcca
     7561 ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa atacccgcaa
     7621 tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga acaccacagc
     7681 ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat gatttaacta
     7741 agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac agataatagg
     7801 ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat ccggtaataa
     7861 tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc tgatcgtttt
     7921 tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca gacgattgat
     7981 tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa acaataaaaa
     8041 aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca ccgccgacat
     8101 aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg gcaaaaaaac
     8161 gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt ggaaccagcg
     8221 accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag gtcacgcaaa
     8281 caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga aataagatta
     8341 cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat cgcgatcgcg
     8401 tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc ctcgccacca
     8461 gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag taatacatga
     8521 ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat ccataactga
     8581 gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac gcccgccttc
     8641 tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag tagcgtttag
     8701 tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca atcaggtcgt
     8761 caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg tccatcatcg
     8821 cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc agcgtctgta
     8881 cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg actttggtcg
     8941 gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct tgtggcaggt
     9001 tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg ccagtccaga
     9061 tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc cgggaggcgt
     9121 ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag cgtttcgggt
     9181 actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc ttaataaacc
     9241 acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc agctttttga
     9301 acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt ggcttcggtg
     9361 cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa ctgccgtcgg
     9421 cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg caacggtaac
     9481 gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt ccctgcaccg
     9541 aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc caggtattca
     9601 tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc gtcatgttct
     9661 cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg cccggcccca
     9721 acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa tcagtcaccg
     9781 tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca tcctctaagg
     9841 cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa tccatcagct
     9901 tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc agggcaatat
     9961 gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta acgaaaatcg
    10021 tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc tcctggcttg
    10081 cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc caggttgagc
    10141 attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat gggaataaat
    10201 tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg cccctctggg
    10261 aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg ccataattct
    10321 gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca gcagcgacga
    10381 aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg agattgagtt
    10441 tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag tgaatgtgcc
    10501 tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca atcagggtca
    10561 tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa cctgtcagag
    10621 catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg ataaagacct
    10681 gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc aacttagccc
    10741 gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa ttcgccagct
    10801 cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct gggaaccgat
    10861 tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg cgcaatttta
    10921 tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga ccatcaacga
    10981 tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc agcgggtcat
    11041 gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc gcgcgttact
    11101 gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc gttcaagtag
    11161 tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat accgcaaact
    11221 tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc agccaaaaaa
    11281 atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca taacgtattg
    11341 tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag cccgaaaagg
    11401 ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa tgatgaaagt
    11461 attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg ccgttgagcg
    11521 tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct cctttgatga
    11581 tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca gctatcaaat
    11641 ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc atgagcgcca
    11701 acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg caatggaccg
    11761 ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg ccgactttat
    11821 cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc cgccactgtt
    11881 cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc caggccacca
    11941 gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact actatggtga
    12001 aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt tgcttgacca
    12061 tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg ccgatcgctc
    12121 ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg cttgcatgac
    12181 cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac aaggtttgat
    12241 gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg gcattatcgg
    12301 gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca gtgaaagccc
    12361 gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca actgcaccta
    12421 tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa cctccgatcg
    12481 tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct atgccgatca
    12541 ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg ccacccactc
    12601 catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac gtgaaggtcg
    12661 tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca ccacctcccc
    12721 gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg gcaacagcgg
    12781 cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg cgatggcgcg
    12841 gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga acaaagaagt
    12901 cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa ccaaactgct
    12961 gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg gcttcaaaga
    13021 tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg ctccgggaat
    13081 gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca ctgcctggct
    13141 tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt tcctgttctc
    13201 tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct ccttcaaaca
    13261 ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg aacaatatcc
    13321 tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg cctggctgaa
    13381 agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg tggcggaaat
    13441 caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg tatccattga
    13501 aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag gaatcccgat
    13561 gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt atttacgctc
    13621 gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag ggactgaaat
    13681 tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc gctgaaggcg
    13741 taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg gcaacgtttt
    13801 cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc ccacgctttt
    13861 ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta taaaaatttg
    13921 taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca attatataac
    13981 catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac tcaggagtac
    14041 atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa aagatgtctg
    14101 gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg cgttattaaa
    14161 ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg ctcatattag
    14221 tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc aaataccagt
    14281 atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag acgtctttcg
    14341 cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg aacgaacacg
    14401 cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc tttcattaat
    14461 gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg aaagcgatat
    14521 tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc caagtctgtt
    14581 aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca cctgccgcat
    14641 gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg tatcacagtc
    14701 ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct acggtatgac
    14761 gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt taaccttaac
    14821 gaagagctat attaataacg gcatcagcga taacccggtc gataataatt caactatcga
    14881 atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc aggagtttaa
    14941 ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc ctgatggtgt
    15001 cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc tccactggcg
    15061 ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc tcgatggtat
    15121 acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac gcccgccgct
    15181 gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc tgctggatcg
    15241 gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc ccgattctga
    15301 aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc gtcctgctga
    15361 acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg ctggcgctga
    15421 tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa acctatatgg
    15481 cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc cttaacgtta
    15541 cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg gtgaaaaacc
    15601 cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc gtttgctatg
    15661 tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc gtttctgcct
    15721 cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc attgtttcct
    15781 tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg gcgggtcaaa
    15841 cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt gtaaataaag
    15901 cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc ttccagctca
    15961 gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc tcggtcatct
    16021 ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc cacggtcact
    16081 ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc tactgcattt
    16141 gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc ctgatggtca
    16201 tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat cccttagatg
    16261 caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca accttaagaa
    16321 cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga tggaaacacc
    16381 gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg gctattgttg
    16441 gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt gttgcaggcg
    16501 ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt tgtcgctttc
    16561 agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg accactggcc
    16621 tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta cggcatcgtc
    16681 atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag ttatgcgctg
    16741 atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga gctgattgcc
    16801 tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt ccgtggagcc
    16861 aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta taaagactac
    16921 gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc taacagcatt
    16981 gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct ggtgcgaatt
    17041 ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa tttgaccatc
    17101 ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta cccgcgtgaa
    17161 actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc atcttgcggc
    17221 acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga gcactacaaa
    17281 gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc gggcatcaac
    17341 gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt gcctcaccag
    17401 aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta tgacgaagtg
    17461 ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt gattgtctta
    17521 cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca gttcaggaaa
    17581 tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca actggtgaac
    17641 acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat taatctgctt
    17701 aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca cggtgaatcg
    17761 ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc ggatagccaa
    17821 aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta tcaggttgac
    17881 cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa tttattctcc
    17941 acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga tgatattctg
    18001 caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat acgctgttat
    18061 tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc gtgacaaccg
    18121 cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta ctggatttag
    18181 ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa aaatacaccc
    18241 tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc gggctggatg
    18301 tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac gcccgtatcc
    18361 gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt ggcaatctga
    18421 cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt ctgacgccca
    18481 aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta catcgggaaa
    18541 ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc ctggaagtgc
    18601 atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg cgcggctttg
    18661 gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt ctgcgccgac
    18721 ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg gtgtttgagc
    18781 tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg tttgagcagg
    18841 cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc gaggcggtcg
    18901 ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc tgctatcagg
    18961 cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa gcgcgcaccg
    19021 ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa gcggtggttt
    19081 cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa aggttgttta
    19141 ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg catctggaac
    19201 tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt gatcagatga
    19261 tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt tcttccggta
    19321 attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac gaactcagta
    19381 ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc gacatcaccg
    19441 ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa aacgcccatc
    19501 gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc ggagcggtca
    19561 tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag ttgagtaaag
    19621 cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt attgtcagcc
    19681 gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag acttccggca
    19741 cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata taaagaaagc
    19801 tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg tttttcattg
    19861 cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg aaccttaaga
    19921 gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct tattcatcaa
    19981 ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca atctcgtggt
    20041 caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt tccttcgctt
    20101 cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc gtctctttca
    20161 tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag gcaggcatca
    20221 tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga ccggcaacca
    20281 gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc gggaacatcg
    20341 ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc agcatcagta
    20401 acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc gggatcgcca
    20461 acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa cggtcactca
    20521 gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata ataatcagca
    20581 ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata taggtcagca
    20641 acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac aggctgcgcc
    20701 agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa ccttcgcggt
    20761 cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc agcgcatggc
    20821 gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata cgccagcccc
    20881 agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg cccgcaccca
    20941 gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca cgtttacggt
    21001 caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg accgagaaac
    21061 cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc gtgtcgtagg
    21121 acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg atagcgagga
    21181 tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca agcggtcgaa
    21241 tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg gggtcagccc
    21301 ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca aaatcaaacc
    21361 attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta ccgtcatcaa
    21421 taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc atagctttcc
    21481 tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc cgcaaaaggg
    21541 gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct tacaccctcc
    21601 ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa tgaccttttt
    21661 accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt aggactcatt
    21721 gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca ttgcccccac
    21781 caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc agcgttgggt
    21841 cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc aacttaaaaa
    21901 atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat ggtaatagtg
    21961 acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt tctcgcgcaa
    22021 atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat cgtcaaatgc
    22081 accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc aggcgttata
    22141 cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct gcaccagtag
    22201 gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc actcatacaa
    22261 ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat ccattttttg
    22321 cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat ccagcatcga
    22381 cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca tgccgccgtt
    22441 cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt cacgaaactg
    22501 ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca ctaaaaagcg
    22561 cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg tcaatatcgt
    22621 tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt gtaaatccag
    22681 ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac cgattcattt
    22741 tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa acatgaagcg
    22801 aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg ctctgccaga
    22861 actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg ttgcaacaca
    22921 ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc gggctgattc
    22981 aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc gtccgttgct
    23041 tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg aactacgccg
    23101 tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc cattttgact
    23161 tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga ttccatctcg
    23221 gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa cagtgtctca
    23281 cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc ggctaactgc
    23341 gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag ggccagctcg
    23401 atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag ctgcaaactt
    23461 tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa cccgtgcgca
    23521 cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg cgcagcagat
    23581 cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg aattggctgg
    23641 gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc gaggtccgcc
    23701 agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc cacccagcgt
    23761 tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc ccgattcgcc
    23821 aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca gcctttcatc
    23881 agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg atcctgttga
    23941 tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa aatcgcctca
    24001 cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc cagtaccgcc
    24061 gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc ggcttcattt
    24121 ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc cgccagatgg
    24181 acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga aaaaggaaaa
    24241 tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag acaatgaaaa
    24301 ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat ccgctgcata
    24361 agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat atcgcaatca
    24421 cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg tgaaaaatgc
    24481 agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc agtcattggg
    24541 cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt tgatttccct
    24601 gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt acacaactca
    24661 ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa agccagttgt
    24721 ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa ctgttggcga
    24781 gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg tgtgtacata
    24841 gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt ttttgagttc
    24901 tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg ttgttttttt
    24961 atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa aatatcatgt
    25021 gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat agaggcaaaa
    25081 agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta aaatcagcag
    25141 tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc aatctgccgt
    25201 tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta ccacactgcc
    25261 caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc ccggaatgtg
    25321 cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc gttaaagatg
    25381 ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat ctgaaggtga
    25441 aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc ctggttgaag
    25501 gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg aaatctgagt
    25561 ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct cgcgccgcat
    25621 ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc agcctctcat
    25681 ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac catcatgccg
    25741 ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta ttatcaacgt
    25801 acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa atcagcgcag
    25861 gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat cgctcatgcc
    25921 aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc aggaaaagcc
    25981 agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg aaaacgctgg
    26041 tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac tttctgggcg
    26101 catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa tgtcgtcaaa
    26161 caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca tcgaattccc
    26221 gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata atccgccacg
    26281 atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt cgagaagctc
    26341 gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat tcatcacagt
    26401 gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt acgtcattta
    26461 agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact gggccgcact
    26521 gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa caccggctac
    26581 atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt gctgattggc
    26641 gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg cgagcagaaa
    26701 cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca tcagcgcgtt
    26761 tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct gatgcagcag
    26821 gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc agcgcgcatc
    26881 gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt cgtcacgctg
    26941 catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg tcaggggcac
    27001 gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca tctctaccgc
    27061 agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca ttgaggaaaa
    27121 cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag cctcagcacc
    27181 ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg cattatttca
    27241 acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga tatggagaag
    27301 aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat tatccagggg
    27361 atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat ggaagcggtg
    27421 gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc gccgggttac
    27481 tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga tctgctggcg
    27541 aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg cttcctcgtc
    27601 cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa gcgcaccgtc
    27661 aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga tgttgccacc
    27721 aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct taaagagctg
    27781 aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg caagaatctg
    27841 tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa aacgccggaa
    27901 gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag cgacctgcaa
    27961 ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa aggcaataaa
    28021 gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca gctggatgac
    28081 ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc ggtgcagtaa
    28141 atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc gttgatgccg
    28201 gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca aaccatcacc
    28261 atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt tctcgacgtg
    28321 ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa agacggcggc
    28381 aacatggcaa cctt
//
LOCUS       DAJTRN010000051        27662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRN010000051 DAJTRN010000000
VERSION     DAJTRN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            16..786
                     /locus_tag="OO328_002230"
     CDS             16..786
                     /locus_tag="OO328_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5930784.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            777..1250
                     /locus_tag="OO328_002231"
     CDS             777..1250
                     /locus_tag="OO328_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5930785.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1357..1896
                     /gene="dnaT"
                     /locus_tag="OO328_002232"
     CDS             1357..1896
                     /gene="dnaT"
                     /locus_tag="OO328_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5930786.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            1899..2636
                     /gene="dnaC"
                     /locus_tag="OO328_002233"
     CDS             1899..2636
                     /gene="dnaC"
                     /locus_tag="OO328_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5930787.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2685..3179
                     /locus_tag="OO328_002234"
     CDS             2685..3179
                     /locus_tag="OO328_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5930788.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3419..5710
                     /gene="opgB"
                     /locus_tag="OO328_002235"
     CDS             3419..5710
                     /gene="opgB"
                     /locus_tag="OO328_002235"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5930789.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(5848..6870)
                     /gene="lgoD"
                     /locus_tag="OO328_002236"
                     /pseudo
     CDS             complement(5848..6870)
                     /gene="lgoD"
                     /locus_tag="OO328_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7009..7923
                     /locus_tag="OO328_002237"
     CDS             7009..7923
                     /locus_tag="OO328_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5930790.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8138..9499
                     /locus_tag="OO328_002238"
     CDS             8138..9499
                     /locus_tag="OO328_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5930791.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9548..11212)
                     /gene="tsr"
                     /locus_tag="OO328_002239"
     CDS             complement(9548..11212)
                     /gene="tsr"
                     /locus_tag="OO328_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5930792.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEISSASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11331..11777)
                     /gene="hpaR"
                     /locus_tag="OO328_002240"
     CDS             complement(11331..11777)
                     /gene="hpaR"
                     /locus_tag="OO328_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5930793.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12049..13338
                     /locus_tag="OO328_002241"
     CDS             12049..13338
                     /locus_tag="OO328_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5930794.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13335..14801
                     /gene="hpaE"
                     /locus_tag="OO328_002242"
     CDS             13335..14801
                     /gene="hpaE"
                     /locus_tag="OO328_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5930795.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            14803..15654
                     /gene="hpaD"
                     /locus_tag="OO328_002243"
     CDS             14803..15654
                     /gene="hpaD"
                     /locus_tag="OO328_002243"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5930796.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15664..16044
                     /locus_tag="OO328_002244"
     CDS             15664..16044
                     /locus_tag="OO328_002244"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5930797.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16112..16915
                     /gene="hpaH"
                     /locus_tag="OO328_002245"
     CDS             16112..16915
                     /gene="hpaH"
                     /locus_tag="OO328_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5930798.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            16926..17729
                     /gene="hpaI"
                     /locus_tag="OO328_002246"
     CDS             16926..17729
                     /gene="hpaI"
                     /locus_tag="OO328_002246"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5930799.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            17751..19106
                     /gene="hpaX"
                     /locus_tag="OO328_002247"
     CDS             17751..19106
                     /gene="hpaX"
                     /locus_tag="OO328_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5930800.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19116..20006
                     /gene="hpaA"
                     /locus_tag="OO328_002248"
     CDS             19116..20006
                     /gene="hpaA"
                     /locus_tag="OO328_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5930801.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20033..20970
                     /locus_tag="OO328_002249"
                     /pseudo
     CDS             20033..20970
                     /locus_tag="OO328_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21197..22755
                     /gene="hpaB"
                     /locus_tag="OO328_002250"
                     /pseudo
     CDS             21197..22755
                     /gene="hpaB"
                     /locus_tag="OO328_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            22773..23285
                     /locus_tag="OO328_002251"
     CDS             22773..23285
                     /locus_tag="OO328_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5930802.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23678..25828
                     /gene="btsT"
                     /locus_tag="OO328_002252"
     CDS             23678..25828
                     /gene="btsT"
                     /locus_tag="OO328_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5930803.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25878..26081
                     /locus_tag="OO328_002253"
     CDS             25878..26081
                     /locus_tag="OO328_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5930804.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26092..27048
                     /gene="yjiA"
                     /locus_tag="OO328_002254"
     CDS             26092..27048
                     /gene="yjiA"
                     /locus_tag="OO328_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5930805.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27117..>27662)
                     /locus_tag="OO328_002255"
     CDS             complement(27117..>27662)
                     /locus_tag="OO328_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5930806.1"
                     /translation="IKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDE
                     DLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQP
                     QEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWG
                     LAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6695 a         7274 c         7020 g         6673 t
ORIGIN      
        1 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
       61 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      121 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      181 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      241 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      301 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      361 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      421 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
      481 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
      541 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
      601 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
      661 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
      721 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
      781 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
      841 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
      901 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
      961 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1021 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1081 cttataccgc aatgatttcg tcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1141 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1201 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1261 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1321 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1381 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1441 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     1501 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     1561 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     1621 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     1681 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     1741 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     1801 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     1861 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     1921 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     1981 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2041 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2101 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2161 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2221 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2281 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2341 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2401 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     2461 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     2521 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     2581 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     2641 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     2701 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     2761 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     2821 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccaag
     2881 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     2941 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3001 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3061 aaacaaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3121 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3181 ccgcgaaatg ttacccggct gacgcggctc tctcctgccc ttttccctgc tcctggacgg
     3241 ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc
     3301 ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg ccattacatt
     3361 gtgcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg atcgcccatt
     3421 gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg catggaaagc
     3481 gggacgtaac acctggtggt ttgcagccac gttaacggtg ctggggctat ttgtcgtttt
     3541 aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg acgcggttct
     3601 ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc tgccgggtat
     3661 cggcattgtg ctggggctga cggcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg
     3721 tcgtcatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg cgctgggttc
     3781 ggtggacgcc agcccggcat ttcgtcagat aacggaactg gtgaaatccc agtcacgcga
     3841 tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc ctgacccgaa
     3901 actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg ataacgaagc
     3961 tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg atttcagcca
     4021 cacacagcag ctgccaggaa cggattacac gattgcgggc atggtggctt ctcagtgcgg
     4081 cataccgctg ttcgccccct ttgaaggcaa cgcctccgcc tctgtctcca gcttcttccc
     4141 gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact atttcgtgca
     4201 gggcgcaaat ctgcgttttg ccggtaaaga tgtgttcctg aaatcgcacg gcttcgacca
     4261 cttatacggc tcagaagagc tgaaaagcgt ggtagccgac ccgcactatc gcaacgactg
     4321 gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag agctttcccg
     4381 ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc cggatggttt
     4441 tatctcgcgc acctgtaacc gcaaaaaata tgattttgac ggtaagccga atcagtcatt
     4501 cagcgcggta agttgcagcc aggagaatat cgcgacgttt atcaacaaaa tcaaagcgtc
     4561 accgtggttt aaagatactg tcatcgtcgt ctcttctgac catttagcga tgaacaacac
     4621 ggcgtggaaa tacctcaata agcaggatcg caataacctg ttttttgtca ttcgtggcga
     4681 caagccgcag caaaagacgc tggcagtgaa gcgtaacacg atggataacg gcgcgacggt
     4741 gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt tatccgggca
     4801 gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga agccagatat
     4861 catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc agcagaaaaa
     4921 catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg tttcagacaa
     4981 acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc agctggccga
     5041 tttcgctcca cgcgacaatt tcgtctgggt tgacagttgc tacaagatgg cacaactctg
     5101 ggctccggaa ctggcacttt ccaccgactg gtgtgtctcg caggggcagc ttggcggtca
     5161 gcaaattgtt cagcatgttg acaaaacaac atggaagagc aagacggcat ttaaagatac
     5221 ggtcatcgac atggcgcgtt acaaaggcaa tgtcgatacg ctgaagattg ttgataacga
     5281 tattcgctac aaagccgaca gtttcatctt taacgtcgcc ggtgcgccag aagaggtgaa
     5341 acagtttagc gggatttccc gtccggagtc gtggggccgc tggtccaacg cgcagctggg
     5401 cgatgaagta aaaatcgagt acaagcatcc gctgccgaag aaatttgacc tggtgattac
     5461 cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag gcaatgaaga
     5521 acaaactctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg ataacccaac
     5581 cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat
     5641 cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag tggtagaacg
     5701 tgaagggtaa ttcctgactg atgtgtaggt cgcatagggt gcgacctaca cgccttctcc
     5761 ggcttacaac acattattaa gaaacaaaaa aaacagcgcc caacacttcc ccggacgctg
     5821 catggcaact ttaataatct cgccttatca gaaagtaatt acacccttaa tcaactcacg
     5881 attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt
     5941 taacatcatg tcggcagtga ttttcccttc cgccatcagt cgaccgactt tagcgaaatc
     6001 ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc
     6061 ggagaactgc aactcacctt taaacagacc gacaaatacc accgtgccgc cgtgacgaat
     6121 cagattcacg gtattattca tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc
     6181 cagcgaacca ccaaactgcg cccgcagctg cgcgtcaaaa tcttcggctg acgggtccag
     6241 cacgggcaac tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac
     6301 caccacctgt gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggccccgc
     6361 cccgaccacc agcacctgct cgccgggagc aatggctgcg cgacgcaccg catgagcgct
     6421 aatagcgaaa ggttcaatca atgcggccgc ctgcgggtca ataccgtctg cgggcaaaat
     6481 gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac
     6541 tgaaattttt tcacagcagt tggtacgccc gcttttacac gccgggcatt gctgacaggc
     6601 aacataaggg atcacagcaa cttgctgacc atttttaaga tcagcaatat ttttacccag
     6661 cccaacaatc tccccacata tttcatggcc taaaacccgt ggataactaa aaaatggttg
     6721 atttccaccc taggcatgaa tatcggtacc gcaaatcccg acagacttaa tttttattaa
     6781 tgcttcattg tcacccggaa taggtatctc gcgttgtttc cagactaatt ctttcggctg
     6841 ctggcaaatt aaaacattca tcgtagacat gattcggcta tccatttttt gttgccttag
     6901 atatcaatga aaatgaaaaa tggcagcgga acggaaaatc tgttttgtga aaacacacgc
     6961 ataaaaatgt tttaaatcgg gttttaatgg actcacaagg aggaaaatat gagtcgttca
     7021 caaaatttac gccacaatgt gattaaccag gtcattgatg atatggcgcg gggtcatatc
     7081 ccttccccac ttccatcgca aagtgcgctg gcggaaatgt acaacatcag ccgcactaca
     7141 gtgcgtcata ttctcagcca attacgcgaa tgcggcgtct taacgcaggt tggaaacgac
     7201 tatgttattg cgcgtaaacc tgaccatgat gacggtttcg cctgtactac cgcttcaatg
     7261 agtgaacaaa acaaagtgtt tgagcaggct tttttcacga tgataaacca gcgtcagtta
     7321 cgccctaggg aaactttttc tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtg
     7381 gttgtaagag aatatctttt aaaattcggc cgttataatc ttattcaaag cgaaaagcgc
     7441 ggccagtgga gcatgaagca attcgatcaa tcctacgccg agcagctgtt tgagctacgc
     7501 gaaatgctcg aaacacattc acttcagcac tttctcaacc tgcctgatca tgatccccgc
     7561 tggctgcagg cgaaaaccat gctagaacgt catcggctac tgcgtgacaa catcggcaat
     7621 agctttcgca tgttctcgca acttgacaga gattttcact ccttactgct ttcagctgcc
     7681 gacaatatct tttttgatca atcgcttgag atcatctcag tcatctttca ttttcactat
     7741 caatgggacg aaagcgatct taaacagcgc aatatcattg ccgtcgatga acatatgacc
     7801 attctcagcg ctctgatttg ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat
     7861 ttgaattcag ctaaacaatc aatgattcga tccattaatg agaacacgcg ttatgctcat
     7921 taagtacgga ttaaaaacag caaactatta gcgggtgcac ggttttatca aaagataaat
     7981 accgggaaat tcccgcttac ctatgctcac aatcagtcga ctaaaaatgt ttttgtgagc
     8041 ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca accttgcgta atgcacaatt
     8101 acgtaaacaa ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa atatcaccct
     8161 cgatccgcgt tcttcattta ctccatcttc gtcggcagat attcccgtgc caccagatgg
     8221 attagttcaa cgcagtaccc gaattaaacg cattcaaacc accgccatgt tgttattatt
     8281 ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa atttaacgat
     8341 tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct ttgctctccg tgttttcact
     8401 cgcttacggc attgcgcaac ttccttgcgg cccactattg gatcgtaaag gcccacgcct
     8461 gatgctggga ctggggatgt tcttctggtc actgttccag gccatgtctg gcatggtgca
     8521 cagctttacg cagttcgtgt tggtgcgtat cggtatgggg attggtgaag cgccgatgaa
     8581 cccatgcggt gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg gacgcccgat
     8641 gggcttcttc aacgcagctt ctaccattgg cgttgctgta agcccaccga ttctggcggc
     8701 aatgatgctg gtgatgggct ggcgcgggat gtttattacc attggtgtac tggggatttt
     8761 tctcgccatc ggctggtata tgctctatcg caaccgcgag cacgtagaac tgactgccgt
     8821 tgaacaagct tatctcaatg caggtagcgt caatgcccgc cgagatccgc tcaattttgc
     8881 cgaatggcgc agcctgttcc gtaaccgcac aatgtgggga atgatgctcg gattcagtgg
     8941 catcaactac actgcgtggc tgtatctggc ctggcttcct ggttacctgc aaacagccta
     9001 taacctggat ttaaaaagca cagggttgat ggcggctatc cctttcctgt ttggggctgc
     9061 cgggatgctg gtcaacggtt acgttactga ctggctggtc aaagggggaa tggctccgat
     9121 taaaagccgt aagatctgca ttattaccgg gatgttctgt tctgccgcct ttacgctgat
     9181 agtaccacaa gcgacaacat ccatgatggc ggttctgctg attggcatgg cactgttctg
     9241 tattcacttt gccggaacat cctgctgggg cttgatccac gtcgcagttg cttctcgcat
     9301 gactgcgtcg gtgggcagta tccagaactt tgccagcttc atctgcgcct ctttcgcgcc
     9361 gatcattact ggttttattg ttgatacaac ccactcattc cgtctggcac taatcatttg
     9421 cggttgcgtc accgcagcgg gagcactggc gtacatcttc ctggttcgcc agccgatcaa
     9481 cgacccgcgt aaagattaaa ctccctatgc ccgataacat tttgcttatc gggcattttt
     9541 atggcgatta aaacgtttcc cagttctccc cgctatctgc cactgccatt ttacgcggcg
     9601 tagctggcgt cacggttttt accacagccg atgtttcacg ctgctgttgc tgctgttgct
     9661 gaatccggaa cactgccaca gcttcggtca ggcgactggc ctgctcttcc agcgcggcgg
     9721 cggcagcggc agattcttcc accagcgcgg cgttctgttg agttacccgg tccatctcag
     9781 caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca gaagatattt
     9841 cgcccataat gtccgtcacg cgggtcacag cactgacaat ctccgccatt gtttccccgg
     9901 cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca atcaggcttt
     9961 taatttcacg aaccgcctgg gcgctacgct gggccagatt acgcacttct cccgcaacca
    10021 ccgcaaaacc acgtccttgc tcacccgcac gcgctgcttc aaccgccgcg ttcaaagcca
    10081 gaatattggt ctggaaggta atgccgtcaa ttacgctgat aatatcggcg attttctgcg
    10141 aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg ccgccgcgtt
    10201 gcgccgtttc agaagcactt aatgccagat ggctggcctg gcgcgcattc tcggcgttct
    10261 gtttcaccgt tgcggtcagt tgctccatgc tggctgccgt ctcttccagc gaagcggcct
    10321 gttgctcggt gcgcgaagag agatcattat tgccggtagc gatttcgctg gcaccgctat
    10381 agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct ccctgcatat
    10441 ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca atcggtttca
    10501 ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt ggcgctacca
    10561 gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg atcatcacgc
    10621 ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg gcgatatcat
    10681 agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg tcctgatatc
    10741 cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt tggatcagct
    10801 ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct gcggcggtgc
    10861 tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc gcctgtttca
    10921 gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca atattattct
    10981 gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc aacaacgcga
    11041 cccagctgcc attcagcgtg ggttgctgct ggcgaatggt ttgtaaaaca gtgaaatttt
    11101 ctttgtcatt ctttaaggca ttaaaaaaca gaccgcctga tgtcagttgt aaaaggccaa
    11161 aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac atggtttctc
    11221 tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat gaaaaggtgt
    11281 aacccgccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag ttattcatta
    11341 tcgcccggga tgtcttcttg tcgggaattg cccagagcaa taaattcctc taacagatgg
    11401 gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata ggcttcttca
    11461 atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggtcagcga gatatagagc
    11521 ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc catccgcgtg
    11581 aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc gtgaaaatcc
    11641 attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt caaattatgc
    11701 cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag caacgcaatg
    11761 gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat atagaaacaa
    11821 ttaatcatca cgttaccggg actgagctgc ccccgcaatt cttcatttta tcaaaattga
    11881 tgatatttta ttaacttact gatatttaat attttattat ctttaaatgt aaatagtttg
    11941 ttaattagat cacatttata tcacttactt ttgccaaact acgcaatcgg gaataaaagt
    12001 aatcattaac atattaatga ttaagatgat cgaatccgag gagtggtaat gaaaggcact
    12061 atcttcgccg tagcgttgaa ccatcgcagc caacttgatg cctggcagga agcgttccag
    12121 caatctccct acaaagcccc gcctaaaact gcggtctggt ttattaaacc gcgcaatacg
    12181 gtgattggtt gcggtgaacc gattcccttt ccacagggtg aaaaagtact gagcggtgcg
    12241 actgttgcgc tgattgtggg gaaaacggcg acgaaagtac gtgaagaaga tgcggcagag
    12301 tacatcgccg gatatgcgct ggataacgac gtcagcctgc cggaagagag cttttaccgt
    12361 ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac cgtggctctc
    12421 agcaatgtcg ataatctgac tatctatacc gagatcaacg ggcgtcctgc cgatcactgg
    12481 aataccgccg atttacaacg taacgccgca cagctgctga gcgccctgag cgaatttgcc
    12541 acgctgaatc caggcgatgc tattctgctc ggcacgccac aggcgcgcgt ggaaatacag
    12601 ccaagcgatc gcgtgcgtgt tctcgcagaa ggtttcccgc cgctggaaaa tccggtagtg
    12661 gacgaacgtg aagtgaccac gcgcaagagc ttcccaacgc tgccacaccc gcacggtacg
    12721 ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg aactggaatt taagccaccg
    12781 gaagagccgc tggtgttcct gaaagcgccg aataccctca ctggcgacaa ccagacctcc
    12841 gtgcgcccga acaatattga atacatgcac tacgaagcgg agctggtggt agttattggc
    12901 aagcaggcgc gtaacgtcag cgaagccgat gccatggatt atgtcgcggg ctacaccgtg
    12961 tgtaacgact acgccattcg cgactatctg gaaaactact accgccctaa cctgcgggtc
    13021 aaaagccgcg acggactgac gccgatgctt tcaaccatcg tgccgaaaga ggcgatcccg
    13081 gacccgcata atctgaccct tcgcaccttc gtcaacggcg agttacgcca gcaaggcacc
    13141 accgccgatc tgatcttcag cgtgcccttc ctgatcgcct acttaagcga atttatgacc
    13201 ctgaatccgg gcgacatgat cgccaccggc acaccaaaag gcttatctga cgtggtgcct
    13261 ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg aattgtgagt
    13321 gaggaaacag cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat gttgcaggta
    13381 acgactactt ccagactacc aatccggcaa cgggtgaagt gctggcggat gtggcctctg
    13441 gcggtgaagc ggagatcaat caggcggtag cggcagcgaa agaggcgttc ccgaaatggg
    13501 ctgatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat ctgatcgacc
    13561 agaacgtgcc tgagatcgcc gcgatggaaa ccgcggacac gggcctgccg atccatcaga
    13621 ccaaaaatgt gttgatccca cgcgcttctc acaactttga atttttcgcg gaagtctgcc
    13681 agcagatgaa cggcaagacc tatccggttg acgacaagat gctcaactac acgctggtgc
    13741 aaccggtagg cgtttgtgcg ctggcgtcgc cgtggaacgt gccgtttatg accgccacct
    13801 ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt gctgaagatg tcggaactct
    13861 ccccgctgac cgccgaccgc ctgggtgagc tggcgctgga agctggtatt ccggcgggcg
    13921 tgctaaacgt ggtacagggc tacggcgcaa ccgcaggcga tgcgctggtc cgtcatcatg
    13981 acgtgcgtgc cgtgtcgttc accggcggta ccgccaccgg gcgcaacatc atgaaaaacg
    14041 ccgggctgaa aaaatactcc atggagctgg gcggtaaatc accggtgctg atttttgaag
    14101 atgccgatat tgagcgtgcg ctggacgccg ccctgttcac catcttctca atcaacggcg
    14161 aacgctgcac cgccggttcg cgcatcttta ttcagcagag catctatcca gaattcgtta
    14221 aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat ccgaataccc
    14281 aggttagggc gcttatcagt cagcaacact gggaaaaagt ctccggctat atccgtctcg
    14341 gcattgaaga aggcgcaacc ctgttggcgg gcggcccgga taaaccgtcc gacctgcctg
    14401 cgcacctgaa aggcggcaac ttcctgcgcc caacggtgct ggcggacgta gataaccgta
    14461 tgcgcgttgc ccaggaagag attttcgggc cggtcgcctg cctgctgccg tttaaagacg
    14521 aagccgaagg cttacgcctg gcaaacgacg tggagtacgg cctcgcgtcg tacatctgga
    14581 cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat tgaagcaggc atggtgttcg
    14641 tcaacaccca gaacgtgcgc gacctgcgcc agccatttgg cggcgtaaaa gcctccggca
    14701 ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag aacgtctgca
    14761 tttccatggg cgaccatcca attccgaaat ggggagtctg atatgggtaa gttagcgtta
    14821 gccgccaaga tcacgcacgt accgtcgatg tatctctctg aactaccagg aaaaaaccac
    14881 ggttgtcgcc agggtgcgat cgacgggcat aaagagatca gcaagcgttg ccgggaaatg
    14941 ggcgtcgata ccattatcgt tttcgatact cactggctgg tcaacagtgc ttatcacatc
    15001 aactgtgcag accattttga aggcgtctac accagcaacg agctgccgca ttttattcgc
    15061 gacatgacct acaactacga gggcaacccg gagttggggc agctaattgc cgaggaagcc
    15121 ttaaagctcg gcgtgcgggc aaaagcgcac aatattccca gcctgaaact ggaatacggc
    15181 acgctggtac cgatgcgcta catgaatgaa gacaagcact tcaaagtggt ctccatttcg
    15241 gctttctgca cggttcacga ttttgccgac agccgcaagc tgggcgaagc gattctgaaa
    15301 gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt atcgcaccgc
    15361 tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga gttcgaccgc
    15421 cagatggacg agcgtgtggt gaagctgtgg cgcgaaggcc agttcaaaga gttttgcaat
    15481 atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga cacggtgatg
    15541 ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat taccgagctg
    15601 ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgctgcccgc gtaaggacgt
    15661 tttatgccgc actttatcgt ggaatgtact gaaaatattc gtgaagaagc ccgcttacct
    15721 gagctgtttg ccagtgttaa cactgcactg gcggcaacgg ggatttttcc gcttggggga
    15781 atacgtagcc gggcgcactg gatcgatacc tggcaaatgg ctgatggcaa gcacgattac
    15841 gcctttgtgc atatgacgct aaaaatcggc agcggacgca gtctggaaag ccgtcaggaa
    15901 gtgggcgaaa tgctgtttga tctgattaaa acgcacttcg cctcactgat ggagagccgt
    15961 tacctagcgc tttcatttga gattgcagag ctgcacccga cgttgaattt caaacagaac
    16021 aacgtacacg cgttgtttaa ataaatttgc accagcttcc aggccggatg ataaggcgct
    16081 gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacacaccc acaccctgat
    16141 cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga tctcgctgga
    16201 ttacccggag atcaccatcg aagacgccta cgccgtgcag cgcgaatggg ttcgcctgaa
    16261 aatcgccgaa agtcgcacgc tgaaaggcca caaaatcggt ctgacctcga aagcgatgca
    16321 ggcaagctcg cagatcagcg aaccggatta cggtgcactg ctggacgaca tgttcttcca
    16381 cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag tggagctggc
    16441 ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg tttacaacgc
    16501 cacggactat gtgatcccgg cgctggagct gatcgacgct cgctgccaca acatcgatcc
    16561 ggaaacccag cgcccgcgca aagtattcga caccatttcc gataacgccg ccaacgccgg
    16621 agtgatcctc ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt ggatctccgc
    16681 cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg tgctgaatca
    16741 tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg tacaactgga
    16801 aaccgggcaa atcattctcg gtggttcgtt caccctcccg gttccggcgc gtaagggcga
    16861 caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg tttaaggaga
    16921 gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgtccgcag attggattat
    16981 ggctggggct gagcagcagc tacagcgcgg agttactggc cggaccagga ttcgactggt
    17041 tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc caactacagg
    17101 cgattgcgcc ctaccccagc cagccagtgg tacgcccatc gtggaacgat ccggtgcaaa
    17161 tcaaacaact gctggacgta ggcacacaaa ccttactggt gccgatggta caaaacgccg
    17221 acgaagcccg taaagcggta cgcgccaccc gttatccccc cgccggtatt cgcggtgtgg
    17281 gcagtgcgct ggctcgcgcc tcgcgctgga atcgcattcc tgattacctg caaaaagcca
    17341 acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag aacttaccgc
    17401 agattctgga cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat ctgagcgccg
    17461 atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt gagcaggcga
    17521 tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat gagcaactgg
    17581 caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac accaccctgc
    17641 tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtaa tagttcttct atttcgatgg
    17701 cgaagcaaaa caacaactct gtctactaaa cgtacgatgt ggagcgcatc atgacgacct
    17761 ctaccctgca atataaagat aataaaactg ttgaaattga acatcgtgtc attaataaat
    17821 tatttcgtcg cctaatcatt tttcttttta ttctgtttgt tttttcgttt cttgatcgca
    17881 tcaatatagg atttgccgga ctgacaatgg gcaaggatct tggcctgacg tccacaatgt
    17941 ttggcctcgc cgcaacactc ttttatgtca cgtacgtcat gtgcgggatc cccagtaata
    18001 tgatgttagc gaaaattggc gctcgtcgat ggatcgctgg gatcatggtg gtctggggaa
    18061 ttgcttccac ctgcacaatg tttgcaacaa gccccgaaac actctatcta ctgcgtatgc
    18121 tggttggtat tgctgaggcg ggttttttac ctggtatttt ggtttatctg acctggtggt
    18181 tcccggcata tcatcgggcc agagccaatg ccttatttat gatagcgatg ccagtcacta
    18241 tgatgctggg attgatcctt tcaggctaca ttctggcctt ggatggactg tggaatctaa
    18301 aaggctggca atggctcttt ttgctggaag gtctgccatc ggtcattctt ggtgtcgtca
    18361 cctggtttta tctcaacgac acaccagacc aggcgacctg gcttgatgaa aacgaaaaac
    18421 aggctttaaa aacgatgatt ctccgtgaac aagagagtgc atttgcccat gcatctgttc
    18481 cacgctcaac cttacgtgaa gtcctgaccc cagccatcat gctatataca cttgcttatt
    18541 tttgcccgac taatactctg agtgcgatta atatctggac gccgcaaatc ctacagagtt
    18601 tcaatactgg cagtagcaac atcatgattg gtttgctggc ggccatcccc caattctgca
    18661 caatttttgg catgatctgg tggagtcgtc gttccgaccg tcttaaagaa cgcaaaaagc
    18721 atacgattct tccctacctg tttgccgctg ccggatggtt actcgcttct gcgacagatc
    18781 acagcctcat tcaactgagc agcatcatta tggcctcaat gggatcattt accgcgatga
    18841 ctatcttttg gacaacaccg gatcaagtta taagcttgca atcgcgtgcc gttgcgatgg
    18901 ctgtcatcaa tgctgtcggc aatgtaggtt cagcagtcag tccattactt atcggtattt
    18961 tgaaagatgc aaccggcagt tttagctcag gtttgtggtt tgtagcagga ttgctggtac
    19021 tgggcgcgtt agtcatgacc cgaatcccta tggggagtcg ggaacactct ctttcatctg
    19081 aacttatcgc accaaaaccg caataaggag tcatgatgtc cagtgaagct atctttgaac
    19141 atggttatga aaatattgat ttggtccggg aatatgatgc ccgctacgct ggtgaagatg
    19201 tgcattatga aacatttgcc cggttagcga atttctttgg acgcgatatg cgccctcact
    19261 ggcacgatag atattttcaa ctccattatc tggtcactgg acgcattact ttacaactgg
    19321 atgagcactt ctattctgtg cgcgcaccgc tttttgtact aacaccacca tcggtgccac
    19381 atactttctt tactgatcct gatagcgatg ggcatgttct gaccgtacgt caggagttaa
    19441 tttggcccct ggtagaaaag ctctggcctg gtagtggaga tgccgcaatg ctacaaggta
    19501 tctgtctgtc actggaggga atggaacata cactggcggc attaaatcat tattggccga
    19561 taatatccct ggagtttcaa caaaataagc aaggacggga aattctattg caatcactgg
    19621 cgcaaagtat ttttacaatc ctactgcgtg aagcaccacc aaatgatatt tcaacatgca
    19681 gcgtgcgcgg cgagatgtgt ttattccaga agtttaatcg gctgattgac gaaaattatc
    19741 gccaacattt aatggtccct gaatatgcaa caatgctcgg tatgagtgaa tcacggctta
    19801 ccgagctgtg ccgacgtttt gccaatcaat cgccgaagag attgattttc gaacgagtga
    19861 gccgggaagc cagacggctt ctgctctata gcgagcaaag catcaaccag atagcccttg
    19921 atcttggcta caaagatccc gcctatttcg cccgtttttt caaccgaatg atgggttgct
    19981 caccaacaca attccgtggt cgctgaccat ttatcaacct gagagactcg acatgaaaat
    20041 atctttacgc tcactatttg tggcgtgcct gattgcgcca gtggcattca cctacgcctc
    20101 cgaaccgcaa taaatgaaaa ttgtactact tggaacgaaa ggtggccctt ctttattaaa
    20161 agctgaccgt ttaccgatgg caacggcact tattattggc aataaaacct ggttaattga
    20221 tgctggttac ggggccagct tacaactggt aaaaaataat attccactgc gccagattga
    20281 taatatttta ctcacccatt tacacagtga tcatattctg gactatccat cagtcttaat
    20341 gaatgcatgg gctagcggtc tgcaggacaa aactattcat gtattcggtc cgccaggcac
    20401 ccgagccatg accgctgcca gttggcaggt cttcgatcgg gacatcacac tgcgtatgga
    20461 agaagaagga aaacccgatc ctcgcaaact cgttacagct caagatgttg gtgaaggcgt
    20521 agtttataaa gatgataccg tgaccgtgac tgcattaaag gtccctcatt ctcctttccc
    20581 tgacggtgag gcttttgctt atcgcttcga cacacaaggc aagcgaattg tcttctcagg
    20641 agatacctcg tactttcccc cactggcaac tttctcagaa ggcgctgata tacttgtgca
    20701 tgaggccgta catgagccct ccgtcgctaa gctggcagac agtattggca atggtaaaac
    20761 actggcaaaa gccattgctt ctcaccatac gactattgag gatgtaggca aaattgccaa
    20821 tgaagcaaaa gtgaaaaaac tcatccttag ccacctggta ccaatcaccg ttccggatga
    20881 aacatggaag caagaagcac agaaaactat tcaggtccta tcgtcgttgg tcatgaaaat
    20941 atgacgatta ccattccatt cgccccataa aaaattccgt ccccgttagc ccttgttagc
    21001 ggggaatatt atgatttgcc ttactgtaag ttgatcacga tcgcagttta ctatttaact
    21061 ccaccttgct gtcgaaaagt accaacgttc gcctgaaacg tctcttcccg acaaccgcgt
    21121 tcaacgcttc aattaaataa caaaaacaac acacaaaatt aacaatcgta ttccgattaa
    21181 tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac gtcctttcac
    21241 cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct atggcgagcg
    21301 agtgaaagac gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg ttgcccaact
    21361 gtacgacgcg ctgcacaaac cggagatgca ggactctctg tgttggaaca ccgacaccgg
    21421 cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg acctgcgcca
    21481 gcaacgcgac gccatcgctg aatggtcacg cctgagctat ggctggatgg gccgtacccc
    21541 agactacaaa gccgctttcg gttgcgcact gggcgcgaat ccgggctttt acggtcagtt
    21601 cgagcagaac gcccgtaact ggtatacccg tattcaggaa actggcctct actttaacca
    21661 cgcgattgtt aacccaccga tcgtcatttg ccgaccgata aagtgaaaga cgtttacatc
    21721 aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt ggttgccacc
    21781 aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt gatgggcgaa
    21841 aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt gaaattaatc
    21901 tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga ctacccgctc
    21961 tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtact gatcccatgg
    22021 gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc gctggacgat ggaaggcggt
    22081 ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact cgacttcatt
    22141 acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg tggtgtgcag
    22201 gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag tgactcgatg
    22261 tgttcagaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc cgcactgcaa
    22321 acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat agaacgcaac
    22381 gttaccagtg gcctgatcta cctcccttcc agtgcccgtg acctgaacaa tccgcagatc
    22441 gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt ccagcgcatc
    22501 aagatcctca aactgatgtg ggacgccatt ggcagcgaat ttggtggtcg tcacgagctg
    22561 tatgaaatca actactccgg tagccaggat gagattcgcc tgcaatgtct gcgccaggca
    22621 caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct gtcggaatac
    22681 gaccagaacg gctggactgt gccgcacctg cacaacaacg acgatatcaa catgctggat
    22741 aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa cgcctgcgct
    22801 ttcatgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc gagggcgacg
    22861 ccggacaatg cgggattacg gcaacggctg tctgctcggt cacggataca ccaccgtcgc
    22921 tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc aacggcaagt
    22981 tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc gcgccacttc gcgggcatga
    23041 caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt ccgctggcgc
    23101 agccggtgct aaaaggttcg ctggccagtc ttgaaggaga gatccgcgat gtgcaggcga
    23161 ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt gcagaaggtc
    23221 acggacttat ttactttaaa cgccgtttcc atccggtgat gctggaaatg gaagctgcga
    23281 tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg atctcaatca
    23341 catatctacc cgatgaataa ttcttgccca accgctcacc gccattcctg accgcttaca
    23401 tccctaaaat aaccactcag ttatttacct tactttacgc gcacgtatct ctggcaacat
    23461 cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag aacggcttcg
    23521 gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc aagtgagtga
    23581 atccccggga gcttacaaaa gtaagtgtca gaggtgaacg aacgcagctg ccgcacctgt
    23641 aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa aactattcaa
    23701 gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt
    23761 acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct
    23821 ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac tcgaccccac
    23881 gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga ccaaccgtta
    23941 cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg tgggaccggt
    24001 tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg gggtagtgct
    24061 ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta acggcgcatc
    24121 tctcggtgag atgatcaaag aagagatggg gccagtaccg gggactatcg cgctgtttgg
    24181 ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg ttaaagccct
    24241 ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat
    24301 gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg ttattggtat
    24361 cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc cgtactgggg
    24421 tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct atgcgtttgt
    24481 ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg caaccttcct
    24541 gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc cggaactgaa
    24601 aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag gcgctctgtt
    24661 cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg cgctgatctc
    24721 ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca ttggctacgg
    24781 cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt ccatcatcga
    24841 accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatca ccatgcctaa
    24901 cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga aagacgttac
    24961 cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag agcagatcct
    25021 gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccgac
    25081 gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg acatgggctt
    25141 ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg
    25201 tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa
    25261 aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg
    25321 ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc tgtggccgct
    25381 gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca ccgttgtgct
    25441 gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg tatggctgct
    25501 tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc agatggaagg
    25561 cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg acctgacggc
    25621 gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg caggcctgag
    25681 tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa cctggcttgc
    25741 ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa tcccggaagg
    25801 cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc ggggctttgt
    25861 tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat ctcggccagg
    25921 cggcgaagat gttgattggt attccagact acgacaacta cgtcgagcat atgaagacca
    25981 accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc cagaacgcgc
    26041 gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag catgaacccg
    26101 attgcagtta ccctactcac cggtttttta ggcgcgggca aaactaccct gctgcgccat
    26161 attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt cggcgaagtc
    26221 tccgttgatg accaactgat tggcgaccgc gccacgcaga tcaaaacgct gaccaacggc
    26281 tgcatctgct gttcgcgctc caacgagctg gaggacgcac tactcgacct gctggataat
    26341 ctcgacaagg gcaatattca gttcgatcgt ctggtgattg aatgcaccgg catggccgat
    26401 cccggcccga ttattcagac ctttttctcc catgagattt tatgccagct ctacctgctg
    26461 gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa ccagttcacc
    26521 atcgcccagt cccaggtggg ctacgccgac cgtattctgc tgactaaaac cgacgtcgca
    26581 ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc accggtctac
    26641 accgtcaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg ttttatgctg
    26701 gaagagaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa acaaaacgat
    26761 atttcgtcaa ttgtggtgga actggattac ccggtagata tcagcgaagt ttcccgcgtg
    26821 atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg gatgctgtgg
    26881 attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta cagcgccgac
    26941 tgggacaggc cgtggggcga tgaaaaaccg catagcacaa tggtgtttat cgggattcag
    27001 ttgccggagg aagagattcg ggcggcgttt gcgggattga ggaagtaagt tcgggaaaga
    27061 tcaacttttt gagagttcca ccgcaacatt attacgagtg gaactcttta gatatgtcag
    27121 accaaccaaa gcttcttcaa ttgcgttttc attttttcat gtctcatttt cagttcagcc
    27181 aatccccatt ctgaagaatt acaaacatct tttgttaaat aaaaagagct tttactatta
    27241 agtttctcat aaatagattt ctttttgata aaatcatagt gttgtgtttc agaatttttg
    27301 gaaccactta tgagtgttaa attacctatt ttatgcagcc agtagacatg ctcttgaggt
    27361 tggaatctat taatccagta ttcattaact aatgcttgag gaagaatatg ctcaattgtg
    27421 attctccccg tataggtttt aatcacgctc tcatcttgtt gctccatatc taagcgaagc
    27481 aaaattgctt ttattaagtt cacttgattc ggacgaggct catataaatc ttcatccaga
    27541 gcagctataa aacctgaatt atctgcatgt tggtttatct gatttatcac agaatcaaaa
    27601 ggcatatcat tatttattgc taccagagca gaataacaaa ccatttctct ttggctttta
    27661 at
//
LOCUS       DAJTRN010000052        27536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRN010000052 DAJTRN010000000
VERSION     DAJTRN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO328_002256"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO328_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5930807.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO328_002257"
     CDS             complement(1614..1937)
                     /locus_tag="OO328_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5930808.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO328_002258"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO328_002258"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5930809.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO328_002259"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO328_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5930810.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO328_002260"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO328_002260"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5930811.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO328_002261"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO328_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5930812.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO328_002262"
     CDS             7537..8574
                     /locus_tag="OO328_002262"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5930813.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO328_002263"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO328_002263"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5930814.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO328_002264"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO328_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5930815.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO328_002265"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO328_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5930816.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO328_002266"
     CDS             10745..11626
                     /locus_tag="OO328_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5930817.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO328_002267"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO328_002267"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5930818.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO328_002268"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO328_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5930819.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO328_002269"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO328_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO328_002270"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO328_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5930820.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO328_002271"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO328_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5930821.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO328_002272"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO328_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5930822.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO328_002273"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO328_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5930823.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO328_002274"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO328_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5930824.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO328_002275"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO328_002275"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5930825.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO328_002276"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO328_002276"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5930826.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO328_002277"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO328_002277"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5930827.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO328_002278"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO328_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5930828.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO328_002279"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO328_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5930829.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO328_002280"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO328_002280"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5930830.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO328_002281"
     CDS             24893..25273
                     /locus_tag="OO328_002281"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5930831.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO328_002282"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO328_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5930832.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO328_002283"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO328_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5930833.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27536
                     /locus_tag="OO328_002284"
                     /pseudo
     CDS             26442..27536
                     /locus_tag="OO328_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6790 a         6853 c         7223 g         6670 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaa
//
LOCUS       DAJTRN010000053        26974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRN010000053 DAJTRN010000000
VERSION     DAJTRN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..816)
                     /locus_tag="OO328_002285"
     CDS             complement(<1..816)
                     /locus_tag="OO328_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5930834.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGG"
     gene            complement(830..2332)
                     /locus_tag="OO328_002286"
     CDS             complement(830..2332)
                     /locus_tag="OO328_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5930835.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(2472..3428)
                     /gene="ytfQ"
                     /locus_tag="OO328_002287"
                     /pseudo
     CDS             complement(2472..3428)
                     /gene="ytfQ"
                     /locus_tag="OO328_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            3738..4268
                     /gene="ppa"
                     /locus_tag="OO328_002288"
     CDS             3738..4268
                     /gene="ppa"
                     /locus_tag="OO328_002288"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5930836.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(4648..4989)
                     /locus_tag="OO328_002289"
     CDS             complement(4648..4989)
                     /locus_tag="OO328_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5930837.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(4992..8771)
                     /gene="tamB"
                     /locus_tag="OO328_002290"
     CDS             complement(4992..8771)
                     /gene="tamB"
                     /locus_tag="OO328_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5930838.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(8768..10501)
                     /gene="tamA"
                     /locus_tag="OO328_002291"
     CDS             complement(8768..10501)
                     /gene="tamA"
                     /locus_tag="OO328_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5930839.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            10707..11345
                     /gene="msrA"
                     /locus_tag="OO328_002292"
     CDS             10707..11345
                     /gene="msrA"
                     /locus_tag="OO328_002292"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5930840.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            11668..13011
                     /locus_tag="OO328_002293"
     CDS             11668..13011
                     /locus_tag="OO328_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5930841.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13073..13279)
                     /locus_tag="OO328_002294"
     CDS             complement(13073..13279)
                     /locus_tag="OO328_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5930842.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            13604..14158
                     /locus_tag="OO328_002295"
     CDS             13604..14158
                     /locus_tag="OO328_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5930843.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(14221..15159)
                     /locus_tag="OO328_002296"
     CDS             complement(14221..15159)
                     /locus_tag="OO328_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930844.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(15371..16111)
                     /gene="cysQ"
                     /locus_tag="OO328_002297"
     CDS             complement(15371..16111)
                     /gene="cysQ"
                     /locus_tag="OO328_002297"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5930845.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            16301..18244
                     /gene="cpdB"
                     /locus_tag="OO328_002298"
     CDS             16301..18244
                     /gene="cpdB"
                     /locus_tag="OO328_002298"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5930846.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(18370..18750)
                     /locus_tag="OO328_002299"
     CDS             complement(18370..18750)
                     /locus_tag="OO328_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5930847.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            18839..19699
                     /gene="qorB"
                     /locus_tag="OO328_002300"
     CDS             18839..19699
                     /gene="qorB"
                     /locus_tag="OO328_002300"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5930848.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            19808..20773
                     /locus_tag="OO328_002301"
     CDS             19808..20773
                     /locus_tag="OO328_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5930849.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            20881..21543
                     /gene="ytfE"
                     /locus_tag="OO328_002302"
     CDS             20881..21543
                     /gene="ytfE"
                     /locus_tag="OO328_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5930850.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(21588..23000)
                     /gene="cycA"
                     /locus_tag="OO328_002303"
                     /gene_synonym="dagA"
     CDS             complement(21588..23000)
                     /gene="cycA"
                     /locus_tag="OO328_002303"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5930851.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(23308..23928)
                     /gene="fklB"
                     /locus_tag="OO328_002304"
     CDS             complement(23308..23928)
                     /gene="fklB"
                     /locus_tag="OO328_002304"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5930852.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            24147..24692
                     /locus_tag="OO328_002305"
     CDS             24147..24692
                     /locus_tag="OO328_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5930853.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(24676..25323)
                     /locus_tag="OO328_002306"
     CDS             complement(24676..25323)
                     /locus_tag="OO328_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5930854.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            25535..26326
                     /locus_tag="OO328_002307"
     CDS             25535..26326
                     /locus_tag="OO328_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5930855.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            26336..>26974
                     /locus_tag="OO328_002308"
     CDS             26336..>26974
                     /locus_tag="OO328_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5930856.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSD"
BASE COUNT         6565 a         7155 c         6659 g         6595 t
ORIGIN      
        1 tccgccgcca atcaccaccg cgagaatggc gtccagctcc agccataatc cggcgttgtt
       61 ggcatcggca ccgcgaatat ccgccgccac gataatgccc gcaatcgccg cacatagccc
      121 gctcaacacg taggtgagca tgacgatgat tcgcgtgttt accccggcat tttttgccgc
      181 ccgaatgttg ataccaacga cttcgataaa catccccagc gccgttttgc gggtcaacag
      241 ccagaacagg agaagcgtca gcaccgcgat aatgaccggc gttggcagga acaacagcga
      301 tccgctgcca aaccatgaga gatccggcga gttaaacgtg acgatctgcc cggcggtgat
      361 cagttgcgcc acgccgcgcc cggcgaccat caggatcagg gtggcgacaa acggctgaat
      421 tttgaggatc gctaccagta tgccgttcca caatcccgcc aggatgccgg tgcccagggc
      481 gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc atcgcagccg ttgtggctcc
      541 ggcgatagcc atcaccgccc ccacagagag atcgatacca ccggtggcga tcaccagcgt
      601 aataccaatc gccagtaacg caacgggagc tgcacggtta agaatgtcga tagggctacc
      661 gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc ggggctacca ggctatcgac
      721 cagcagcacc agcaacagtg ccgccagctg cggcatcccc gtcggccagt gaaaacgcct
      781 ttttggcggc gtagtgtcag ggagagattg aggcatcaca ctgttctcct tacgccgcaa
      841 tggcgttcat gatcgccgga acggaaagct gtgccagcgg gatctccgcc acctgtttgc
      901 gatcgcgcat gatgatcacc cggtcggcat agcccaccag ctcttccagt tcggaggaga
      961 tcaccagcag cgccagaccg tcagcacaca gcgtttcaat caggcggatg atctcggcgt
     1021 gggcaccaac atcaatgcca cgggttagct catcaaggat cagaaattgc ggtcgggtca
     1081 gtagccaacg tgaaagcagc actttttgct gattgccacc ggagagaaat tcaatcggtt
     1141 gttcagttga aggtgtgcga atgccaagct ggcggataaa gcgttcggca atctcttgct
     1201 gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc ctggagagcg aggatgatat
     1261 tttcccgcac cgaggcggcg gcgatgatgc catcggtttt cctgtcttcc gggcagaatc
     1321 cgatgcccag tactgaagcc tgatgtggcg atcgcagggt ttgcggtttg cctttgatca
     1381 acgccgtgcc gctgtcagca ggtttgatac cgaagatcac ttcggcggtt tcggtacgtc
     1441 cggatcccag caagccagcc agaccgacga tctcgccggg gcgcacttcg agatcaaacg
     1501 gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc aacgggtttg tcgctcaaca
     1561 atgttcgccc ggcacgctgt agcgcgtggg tatccagctc gcgccccagc atcattttta
     1621 ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc tacgaaactg ccgttgcgta
     1681 agacggtgat ccgatcgctg acctgataga cctgatcgag aaagtgagtg acaaagatca
     1741 ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa taatagctcc atttcctggg
     1801 tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt ggcggagaga tcgatagccc
     1861 ggcaaatggc gacgatttgc tgcatcgcca ccgaaaaacg gttaagcggt tcgcgcacgt
     1921 cgagggagaa accgtaagat gccatcagtt cggtggcgcg cttttccatc tctttacggc
     1981 gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag attatcagca accgacatat
     2041 tgggtagcag gttgacttcc tgatagacgg tgccgatacc gagttgttgt gcatgggcgg
     2101 tatttttcgg tgagatagtc tggccttcca gccagatggt gccgcgatcg gcgtggtata
     2161 caccagttaa tgctttgatt agcgttgatt ttcccgcccc gttttcacca agcagcgcca
     2221 tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa cgctttgacg ccggggaaaa
     2281 atttacttaa tccttcggtg cggaggatct cctggtgttg gtcggtcgtc atggtttccc
     2341 ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg gtaagcgtcg catcaggctt
     2401 cagtgcacca tcgccggatg cggcgcgagc gccttatccg gcctacgaat cggcatcagg
     2461 catagcaacc ctcaataccc catatttttc ttcttctcta actcttcttt tgcggtatca
     2521 ggcaggtaaa gggtggattt ggttaacgtc agcttttcag gcatggtgcc gtcttttttg
     2581 tatttctcca gcgcatcgaa agcgggtccc gccatgttcg gcgtcagttc aacactggcg
     2641 ttcgcttcgc catccatcat cgctttgtag atgtccggta caccgtcaat ggaacccgtg
     2701 aggatatctt tgcccggttt caggcccgct tctttaattg cctgaattgc accaatcacc
     2761 atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc cgttgttttc cgctttgata
     2821 aagctctcca tgacttcttt gcctttactg cgggtgaagt cacctgactg cgagcggata
     2881 attttgatat ttggcgcatt cttaatggct tcggcaaagc ctttcttacg gtcaatggcg
     2941 acgctggccc caacggtgcc ctgcagctcc accacgttgc atggtttgcc attcacttct
     3001 tttaccagcc agtcaccgat caacttacct tcgaggatgt tgtcggcagt aacggtggtc
     3061 atatagagag atttgtcttt cacatcgatg gaacggtcga gcaagaagac cgggatttcg
     3121 gcatctttcg cctcttttaa taccggttcc caacccgtcg caaccactgg agcgataaag
     3181 atcgcatcca ccccttatgc aacgaaggaa cgtaccgctt taatctggtt ttcctgcttt
     3241 tgctgaccat cggcaatttt caacgtaatt ccgcgttttt cggcctcact tttcgccaca
     3301 ttggtttctg cggcgcgcca gccggattcc gatccgacct gcgaaaatcc tacggttaat
     3361 ggagcggcca acgccataga cgacatggct gccgagactg cagagactac aagtaagcgt
     3421 ttccacatag ggttgtcctc gtcggggtaa ttattattgg tgaaaagatg ttcgcgaaaa
     3481 aactatagac aattcgttat gtaacggatt gcgttacatc acacttcaaa aaagtaaata
     3541 aaacgttaat cacaagtttg taatcgcttt catctcacta tgaaaaatgc ggctacggtt
     3601 atggattttc ctgctctgta taccgtctta aaactggcga aaaaggaaaa tgaagacgaa
     3661 aacaagcaaa gacattcggc gcgagttggc tataatactt ggcacttgtt tgccacatat
     3721 ttttaaagga aacagacatg agcttactca acgtccctgc gggtaaagat ctgccggaag
     3781 acatctacgt tgttattgag atcccggcta acgcagatcc gatcaaatac gaaatcgaca
     3841 aagagagcgg cgcactgttc gttgaccgct tcatgtccac cgcgatgttc tatccgtgca
     3901 actacggtta catcaaccac accctgtctc tggacggtga cccggttgac gtactggtcc
     3961 cgactccgta cccgctgcag cctggttctg tgatccgttg ccgtccggtt gccgttctga
     4021 aaatgaccga cgaagccggt gaagatgcga aactggttgc ggttccgcac agcaagctga
     4081 gcaaagaata cgatcacatt aaagacgtaa acgatctgcc tgaactgctg aaagcgcaaa
     4141 tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg caagtgggtg aaagttgaag
     4201 gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc ttccttcgag cgcgcaaaga
     4261 ataaataagt tcttctagcg caataaccct gaacgccggg cttcggttag taagggtttt
     4321 tttatgcccg cgataaataa actctctatt ccaccatcat tattctcagc ggttgcaagg
     4381 cttgaacggt aagaacaagc aaacccgacc accattttgc tgttcatagc cacttgctgg
     4441 aagttagccg acctcactca tactcaccgt tcaaagcgtg cccccgtttt acccctgcac
     4501 atttgtgaac gccagcagag ttttttatgc gattgcagat atggaaagcg gctcggagag
     4561 aaaagcaaaa gatggaaaag ttacaaaaaa aaatcgcgag atacatcgca taaaacaacg
     4621 ccacccgaag gaggcgtgct tttattatta cttatccctg tctaaccagt cgccgctttc
     4681 aattagcttt aatccatcga cgggtcgttg ataaacgtac atccatgcac tcccatacgg
     4741 cgtctgaatc aactggcgcg cgtattcacc gcccctggtg cgcaaggcat caagttcggc
     4801 cagcgtggcg ttgtcaatac gataaacttc accgtgtacc gttccgttcc ccggaactgc
     4861 gcctggatag tggcccaggc tatacaactg gtagttatcg atactgaaat cgcccagtaa
     4921 ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg cgtaaactgc cgtagacaaa
     4981 tattcgcatt gctaaaactc gaactgatag agcaaatcca gtgcctggtc tacaccagac
     5041 acggcttcca gatatagctt aggcatcagg cgataacgta acgtgagtgt tgctatagag
     5101 tcaaatatac ccacgccgta tttcacttgc agacctggca atacatagcc gctgaccact
     5161 acctgggagg agtcgcctac tcccagggtg tcgagcgcta aattgcttac gccaaacgtc
     5221 tcgccgattt tacccacaat ctggccactt tgcgcaaccc ccagaccaat cagcatcgag
     5281 gtcattgccg cactgtcgct ctgatcgctc tccagccctt gtccacgtag caaataagac
     5341 aatgcagctt gttgcgacat cgccgggtca gagaagatct ccgctttcgg ttcgtccgcc
     5401 agaccagtga cgcgaactcc ggcgattaca tcgtcttctg tagcatccgg gttacgaata
     5461 gcttcaatat taagatacgg ttgatccggc ggaccagaga acagtaactc acccttacgc
     5521 acaatcagat cctgaccata ggcatggaag cgcccttcag ggatgttgat ctgcccgttc
     5581 aggcccagcc cttgtttgtc ctgtacgacg ttaagatcgc ccgtcagccg cgctttcagg
     5641 ccaaaggcgt caatgcgcac attgttgcca acgtggacaa tcaggttact gttaatcgga
     5701 atcgacgccg ttttcggctc ttccggttgc aggttatcgt taagcatcac cacatcgctg
     5761 gagacgccta ctgcgctttc cggcagatcg tgcaccacga tgcgcgccca cgggacatcc
     5821 acgcgaccat cgagggtaaa caggtttggt gtagcctcga atacaacatc tggcgataca
     5881 tccattcgta ccatcggcgg cacggtgatc cgcactttac tgcctttcgc cgttactcgt
     5941 gcccgccagt tttcaatttg gctccagtcg gcatcaccgt tcaggtagat ttcaccctgc
     6001 tgggtccgta ctgtaccggc aagcgtcgag cgcataccgt taaagttgac cgcaagctgg
     6061 ctcggctgca tatcaaacgg cataaagttg ccgtcgatat ccacacccgt aacctgaagc
     6121 tgaccaaaca actgcgggct ttgcacatca ccacccagac gcaagttggc actcaccatc
     6181 cccgctgctt tttccccacg ggtaaagatg gggtttatca tcgcaaggtt gaagttacgg
     6241 atattgacgt tgccaccaag attacggcgg ccttgcggat cggtcacctg cacctgtcca
     6301 tcaaactggc cgttattggt caggcggatg gtccagccca attcggcacg gttgttacgc
     6361 aattccgccg tcagattcag tgtctgaaac gccaccggca gcgccgcatc gttgacggtt
     6421 tgcgttacct gcacgttacg ccccgaaagg gtgatactgc cctgcggcag cccctctttc
     6481 gtggtgtccc aggcaacatc cgctttaccc gtgaagatac cgctggcctg agtggtttct
     6541 ggcataaacg gtttcagcat ggcgaggtcg aagcggttga gattcaccac cgcacgccct
     6601 tcggcccccg catcgatagt ttgcggcacg cacagttccg cattcgggtt aagccagcag
     6661 tgtggcccga tgctgatttt ttgctcctta ttgcggtaat ccagcgcaat atcgcgggtc
     6721 agcgaccacg ggccgaccgg cgtctggaag cgggtattgc taagagttcc cttccagcgt
     6781 tcttctttgc gatcaaaact tcctgccaga ttaagctgcc cggagacagg ctcgccctga
     6841 atccgcaact gtagctcgtg ctgcttttcg ctgcctttgg cattcagggt gacgaggttg
     6901 atatttacat ccggctgcga aatttgctca acgcgtacgt cgagtttccc ggcgatctga
     6961 tcggtggatt taatgtcgcc ttccacgcga acctgcgcca cggaaagttc ctgccagcgc
     7021 aggccgcgcg cggtgatatc tgccagtagt tgtggcgctt ccaccgtgcc gcgtactttc
     7081 accagccctt tcgctgtacc gccaagcccc ggcagcgcgt tatccagccc cggcgcgttg
     7141 atggtggcat caagattgag atcttttacc cccagctcgc ctttcacttc ggcactgttt
     7201 ggcccgagtt ccagatgaag cccagggatc atccactgca tataactgtt gcctttcagc
     7261 gtgccgtcaa cgttcacttt gttctgtttg acgttaccgg tcagcttcag ttctggcacc
     7321 tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt taatcaagcc attgagtttc
     7381 gacggccact ccgggaactc tttggcggtg ttaatgccat taagcgttag ctcaccgcgc
     7441 caactaatgg cctgctgcca gtcgagcaac gccttgagtt cagttttccc ttccagcgcc
     7501 gcgacggtga gtttgtcgag attgacctgc tgttcattac ctttggcatc aagggtaatg
     7561 gtggctggcg ggatctcctg tcccttcact gccgtacgca tagagagcgt gtaatcggtc
     7621 attttgccgg taagtttcag tttcagatca tccgcctgat actgcttctc accagtgaac
     7681 ggccagtaaa gctgtttgct gttcacttcc acgttgagtg gcaatccggc ttccgccagt
     7741 cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa ggttaacgcc tatctccagc
     7801 tgttcgcgca gcgcgccgcc cactttcagc ttcacctttt cacctttcaa cggctccacg
     7861 ttcagtgtgc tgttgagggt gatatccacc ggccagttgt ctgacagctg cgccgtgccg
     7921 ctggcgttga cgatcccctg gttggaatcg atatccaggg cgtccagttt agtattgccg
     7981 tcaatgctgc tcactttcag cagcatggtg cgcacggtga tgtccgtgtc gcccgtcacg
     8041 cgcagctgct cgcccttaaa ctcttcaatg ttcaggttaa gcggcaaatg cacgtcggtc
     8101 atttccggca ataccgggcg agaaaagaga tctttcagcg tttcgccgag cggcttttcc
     8161 tccggctgcg gattttcaat tttcggttcg accacttctt cctgcgccac ttccaccact
     8221 ttcggcagag caatcagcag gcctttcagc gacgtcggtt tcagggtcag ggttttctcc
     8281 tgccagttca ggccggaggt gaagtccatc accgacaccg tggtgtcatc aatcttgatg
     8341 ttgacgttgt ccagcgccac ccgtgtcagg gtgatgggat acggcgtgga gagatccagc
     8401 ggaccgctat cttcctcttc ttcaacctgt tcagaaggag gcattttttt actgtcgatg
     8461 ttgacctgaa tgtctttcag cgccaggtca ttaatacaaa cgctactgtt ccacaggcac
     8521 tcaagaccga cccccagatg cagattaccc gcttttaccg ccacgcctag ctgctcataa
     8581 cgaacgtcag acaaggtgag atcgcgccag ccgccggtga ccttgccaat atccagtcct
     8641 ggcacccagc gatccgccgc tttaaataca agatgcaggc cgctggtggt gcccaccaga
     8701 aacgccaccg atcccagcaa cagtaagata acgataacca cgccgaggct gatttttttc
     8761 cataaactca taattctggc cccagaccga tgtaaaactg taacccgtgt tcgtctttat
     8821 ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac cggcgattcc cagcgtacgc
     8881 cgaccccggt accggtttta aagtcgctgc ggcgaatatc gcttaccgct tcgccactat
     8941 cgacaaacac cgcgccccac cattttccgg tcacgttgta ctggtattcc agcgatccgg
     9001 ttatcaactt cgaggcccct ttcaggtcac cgttggcgta tttcggggcg atagatttgt
     9061 atttgtagcc gcgaatactg cgatcgcccc cgacgaagaa acgcagatcc ggcggtactt
     9121 tgtcgaaatc accggtttca atccagccca gcgtgccgcg tgtcacaaag cgatggcgat
     9181 cgtacagtgt gcggatccag acgtgctgcg cctggaaaac ggagaaatcg acatctgaac
     9241 cccaggccgt gttggagtag tcgatagagt agcgttgcga gtctccccag gttggcatca
     9301 ggccaccacg agaacgcgtg cggctaatca tcaccccagg ataaaacagc atcgtggtgt
     9361 tggtaatttc accctgggta aagtggtcga ggctccagcg caggttaatg gcacgctgcc
     9421 agccgctgga gagatcccag tagcgagaag ccaccagcgt agtggagtca gattcggtat
     9481 cgttcaggtc agtgcgctta aaaccgccct gcaccaaata atattgttcc agtggattct
     9541 tcagcagcgg cattttatag ctgaagtcga gggtctgttc cggcgcggaa atactggtac
     9601 tggtggtcaa actgtgaccg tatgagttca tccacggctt tttccacgtc gctttcacgc
     9661 gcggtcccac gtccgtagag taaccgaccc cggtttcgat ggtgttttct gttcgcggcg
     9721 aaaccacgcc cgtcaatggt aatactttcg tttcgcgcgc tttatcaaat tgtggagcca
     9781 ccaccaccga gttaaaccag ccggtagcag aaagtcggcg gttcagttcc gccagatctt
     9841 tcgattcata ctcatcgccc tctttaaacg gcaccagatt ttgcaggtat tcatcgcgga
     9901 tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg ttcgccactg ttataatcaa
     9961 tatcccagaa ggctttatgc aggccgagcg caatgcccag ctgcgctttg gtaaattcgc
    10021 tatcgaaata acctttacgc aacgcaatac tggttaagga ctttttgaaa ttttcataat
    10081 cgccctggtt cagtaccgta ccaatagccg ggcgagtatc gagcaatttc aaatagtctt
    10141 tatcggtccg cgcgccgccg cgcaatacca catcggtgcc gccaattaac accggcacac
    10201 ctggcgtgac tttggcgatc aatacctgcc gccctttctt tggcggtgga cggagatcaa
    10261 attcaatggt cggctggtaa taacccagcg ctttcagacc ttcacggatg gcatcatcga
    10321 cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact ttcaatcgta gaaagctgcg
    10381 cacgaacgtt cttttccagc tgtcccgata acccctcgac ctgtagacgg acgttcgcgg
    10441 cgacggcaga tccgcttaag cagagtaagc ttacacagca taactgtcgg atatagcgca
    10501 cattttctcc tgaatatcct tttttcctgc ccctggaacg ccgttaaacg gcttaacaaa
    10561 aatccagtaa tatggattaa aaagcagact aaaccccaaa tatttcttat gttttacttt
    10621 agacctattc acggtggtta ttgtgtgcaa atacgcctct tgttacaacc ttaaccccaa
    10681 tgaccgattt tcgggagagc gacaccatga gtttatttga taaaaagcat ctggtttccc
    10741 ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt agccacgctg catgcggtca
    10801 acggtcactc aatgaccaat gtacctgacg gaatggagat tgccattttt gcgatgggtt
    10861 gtttctgggg tgtggagcgt ctgttctggc agttacccgg cgtttacagc accgccgcag
    10921 gctataccgg cggctatacg ccaaatccga cttatcggga agtgtgctcc ggtgatacgg
    10981 gtcatgccga agcggtacgc attgtttacg atccttccgt catcagctat gagcagttgc
    11041 tacaggtatt ttgggagaat cacgatcccg cccagggaat gcgtcagggc aatgaccacg
    11101 gcacgcagta tcgttcagcg atttatccgc tgaccccaga acaggatgcc gcagctcgcg
    11161 ccagtctgga acgttttcag gcggcgatgc ttgccgccga tgacgatcgt cacatcacca
    11221 cggaaatcgc taacgccaca ccgttttatt atgccgaaga tgaccaccag caatatctgc
    11281 ataaaaaccc atatggttat tgtggaattg gcggaattgg cgtctgtctg ccgccggaag
    11341 catagcgtta cgggtacaaa tgtagtttgt tgataaagtg ctctttgtct atgcctgatg
    11401 cggcttaaac gacttaccag gcctacaaaa tcgcccaaat tcaatatatt gcaaggactg
    11461 cgtaggcctg atcggcatag cgcatcaggc agtattgcat ttatcagcag tctgaatttt
    11521 aaccctctgg cgactttaca gtaccttacg ctatactagc cactgaaaat gccggttcac
    11581 tttcttcgaa tcggctttca atgtgtattt cacacaaatt aatcaacttt cccttccgag
    11641 ggtctggcct gaaaggtcgg ataagatatg ttaaacagta ttttagtcat actctgcttg
    11701 atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc ttgccgcctc acgcaaaatc
    11761 aaacttaaac tgctggctga tgaaggcaat ataaatgccc aacgcgttct gaatatgcag
    11821 gaaaatcccg gcatgttctt taccgtggtc caaatcggtc tgaacgcagt ggcgattctc
    11881 ggcggtatcg tcggtgatgc ggcattttct ccagcttttc acagcctgtt ctcccgctat
    11941 atgtcggcag agctctctga gcaactgagc tttattctct ctttctcgtt agtgactggc
    12001 atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg gtatgattgc gccagaagcg
    12061 gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt acgtttgcac cccgctggtg
    12121 tggttcttca acggcctggc gaacatgatc ttccgtattt tcaaactgcc aatggtacgt
    12181 aaagatgaca tcacttctga tgacatctac gcggtagtgg aagccggtgc gctggcgggc
    12241 gtgttacgta aacaggaaca cgagctgatt gaaaacgtct ttgagctgga atcccgtacc
    12301 gttccgtctt caatgacacc gcgtgaaaac gtgatttggt ttgatctcca cgaagatgag
    12361 caaagcctga agaataaggt ggcggaacat ccgcactcta agttcctcgt ctgtaatgaa
    12421 gatattgacc acatcatcgg ttatgtcgat tctaaagacc tgctgaaccg cgtgctggct
    12481 aaccaaagcc tggcactgaa cagcggcgta caaattcgca acacgctgat tgtgccggat
    12541 acgttaaccc tttcagaggc gttggaaagt tttaaaaccg caggtgaaga cttcgcggtg
    12601 atcatgaacg agtacgcgct ggtggtgggg atcatcaccc tcaacgacgt gatgaccacg
    12661 ctgatgggcg atctggtcgg tcaggggctg gaagagcaga ttgtcgcccg tgatgagaac
    12721 tcatggctga ttgacggcgg caccccaatt gacgacgtca tgcgcgtgct ggatattgac
    12781 gagttcccgc agtcgggcaa ctacgaaacc atcggcggct ttatgatgtt tatgctgcgt
    12841 aagatcccga aacgcaccga ttcggtgaaa ttcgccggct acaaatttga agtggtggat
    12901 atcgataact accgcatcga ccagctgctg gtgacccgga tcgacagcaa ggccaccgcc
    12961 ctttcgccaa aactgcctga cgctaaagat aaagaagaaa gcgtcgcgta acccccagaa
    13021 acatcaacgg ctcctgaatc aggagccgtc ttattactgc atagcacttt ggttaagcca
    13081 tctctgtttg cagacgcata acctgacggt taacttcgga catcactgac aaatgcagct
    13141 tatccttcac ttttgggata agaatcttac ccttatcaaa ttcaaaagcg ccaatgtcct
    13201 tgatgtataa ccgtccacgg aacaggatct ttacgtactt cgccacctga agtgggttgt
    13261 accgttggaa aattttcatt gttatctcct gctgagtatt acgcctgtgc ggtgccacaa
    13321 tcggcccaac tattatgagg cgcaaatttt aatgcttagt gactatagac tatccgggca
    13381 atgtttccac cgcgtataac tttttttacc taaaggttac aattattcag aattatcttt
    13441 ttaccgaagc gcggtcttca gtataagcat tcatttttca tatgatttgt gcgcttgacc
    13501 gcaaactggc atcacacttg cgggaaattc gataaatagc acatatgatt aaaactcaga
    13561 cccaagtggt cggatcacct gcatatcata agaaggaaac accatgaccc tacgcaagat
    13621 tctggcactc acctgcctgc tgttgccgat gatggcttcc gcacatcagt tcgaaatcgg
    13681 tcagcgagtg ccgccgattg gcattaccga tcggggcgag ttggtgcttg ataaagatca
    13741 gtttagctac aaaacctgga acagcgcgca gttagtggga aaagtgcgag tactgcaaca
    13801 tattgctggt cgcacctctg caaaagagaa aaacgcgacg ctgattgaag cgattaaatc
    13861 agcgaagtta ccgcacgatc gttaccagac caccaccatt gttaacaccg acgacgcgat
    13921 tccgggttcc ggcatgtttg tgcgcagcag tctggagagt aataaaaagc tttatccctg
    13981 gtcgcagttt attgtcgata gcaatggcgt cgcgcgcggt gcctggcagc tggatgaaga
    14041 aagttccgct gtagtggtgc tggataaaga cggtcgcgtg caatgggcca aagacggggc
    14101 gctcacaccg gaagaggtgc agcaagtgat ggacctgctg caaaagttgc tcaaataatg
    14161 gcaataacat aaattctgga aaaataattt tcccctatcg ttatctatgg gataggggaa
    14221 ttaagtctct gagttcttac accgaactaa tactggtaac gctagttgct cttttttaaa
    14281 cgcctcaatg gtcgatttat atttctcgca aatcttttct ttggtaatat tactatctgc
    14341 ggccagattc gcacttacta tcgcatccac ttgttcggct aattcgactt gttggcaacc
    14401 tgcattaacc agaaaattat aaataagaga gtaatcttca tcctccaaaa caacagagga
    14461 accattacga aaacgttcat cccctccata gattttaagc gcaaagccca aagattctat
    14521 gactatggca aaattatcat caattatttt ttcgtaaata taccatccat cgcattcttt
    14581 aattccaccg cctggtattt ggagttcaaa tttaccccgt tttaatttgc ttactacatc
    14641 ctccaataag gcataaccga aagatacgtc accaaacgcc tggcgaatat aacctttaat
    14701 cctttttggt atgtcagact gttttttctg caaaatatca caggcataca atcttctgtt
    14761 ttgttctaac ggaaactcgc ggtttttgaa atccatgaat gagttatact catttattgt
    14821 tatgggaatt tcttcgtatt caaacccact gcctcgcaat gttattgtat attcagcacc
    14881 gttatcttta aggctgaaac tgataagaaa attatccatg gcattaacag tttcattttt
    14941 tgatgaaagc acttgataac tatttttatc aatctgtgtt gcgcaacgaa aggactcccg
    15001 caaagttaac agactatcat aattcgaaac ccaatctttt ttcttaaatg catgctgaat
    15061 attattaaaa aaatcgtttg aatgtgtttt tgtatgcgat gactttacaa ataactccac
    15121 cgcaccaata aaattattgt gtctgatcct gggtaacata tggctacctt attttatttg
    15181 gttattttat gtcgccatta aatatctcat tatagaagta aactatcaaa aaacgctcat
    15241 tattaaaatg taacttacta gcaattgaat ttttgcactt taaatacaat ctctttttat
    15301 ttataacttt ttaggttggg ataaatcagg atggcagaat caggaaatac actgtttctg
    15361 ccatctgaat ttagtaaatg gacactctga accccggatt caggaacgac tcgcgcggag
    15421 tgtaatccag cggtttaccc tgccagtcgt gaacgtgcgc tccggcagct gcagccacag
    15481 catgtccggc ggcggtgtcc caaatattcg ttggtccgaa gcgcgggtac agctgcgcct
    15541 gtccttccgc caccaggcag aatttcagcg aagagccgat ggacgtggtc tgatgttcgc
    15601 caagctgttg cagatactct ttcagctccg catctgcatg ggaacggctg ataaccacca
    15661 gcggcgggcg cgcatcgcgg acctgaatca gcttgcgcac accgcactct tctttccagg
    15721 ccttgccttc tgccgcgctg tacattacgt tcattaccgg cgcatacacc acacctaaaa
    15781 tcggtttgcc atggtcaatg agcgcaatgt taacggtgaa ttcgccatta cgtttaataa
    15841 actctttagt accatccagc gggtctacca gccagtaacg ctgccagtgc tgacggactt
    15901 cccaaccggg aggatcttct tcagaaagga ccggaatatc cggtgccagc gtacgtaaac
    15961 cgtccatgat aacggtgtga gcggcaatat ccgctgccgt taccggagaa ttgtccgctt
    16021 tgctgacgac gtccatcggt ttcgtcccgt cgtagacctg cataatggca tcgcctgcat
    16081 tccgtgcaag ctggcatact tgatctaaca tttctccacc tcgtctctgt gagcggtgtt
    16141 aacttattgt tttacttata ccctatcgtt aatgaatgcg ccaactgtga tagtgtcatc
    16201 attttcaaag cgtaaaattg tggcattctt cactgttcta taagtaaggc gtttattctc
    16261 ccttttcttt cgtattcccg atgataaaag gatgtccctg atgattaagt ttagcgcaac
    16321 gctcctggcc acgctgattg ccgccagtgt gaatgcagcg acggtcgatc tgcgtatcat
    16381 ggaaaccact gatctgcata gcaacatgat ggatttcgat tattacaaag acaccgccac
    16441 ggaaaaattc ggactggtac gtacggcaag cctgattaac gatgcccgca atgaagtgaa
    16501 aaacagcgta ctggtcgata acggcgattt gattcagggg agtccgctgg ccgattacat
    16561 gtcggcgaaa ggattaaaag caggtgatgt tcatccggtt tataaggcgc tgaatacgct
    16621 ggattatacg gtcggtacac tcggcaatca tgaatttaac tacggtctgg attacctgaa
    16681 aaatgcgctg gcgggagcga aattccctta tgtaaatgcc aacgtcattg acgccagaac
    16741 caaacagcca atgtttacac cgtatttaat taaagatacc gaagtggtcg ataaagacgg
    16801 aaaaaaacag acgctgaaga ttggctatat tggcgtcgtg ccgccgcaaa tcatgggctg
    16861 ggataaagct aatttatccg gaaaagtgac ggtgaatgat attaccgaaa ccgtgcgcaa
    16921 atacgtgcct gaaatgcgcg agaaaggtgc cgatgtcatt gtcgttctgg cgcattccgg
    16981 gctgtctgcc gatccgtata aagtaatggc ggaaaactca gtttattacc tcagtgaaat
    17041 tccgggcgtt aacgccatta tgtttggcca tgctcacgcc gttttcccgg gtaaagattt
    17101 tgctgatatc gaaggggctg atatcaccaa aggcacgctg aatggtgttc cggcggtaat
    17161 gccgggcatg tggggcgatc atcttggggt ggtcgactta caactcagta atgacagcgg
    17221 taaatggcag gtgacgcagg cgaaagcgga agcacggccg atttacgaca tcgccaataa
    17281 aaaatccctc gcggcggaag acagcaagct ggtagaaaca ctcaaagccg atcacgatgc
    17341 cacacgccag ttcgtcagca agccaatcgg taaatctgcc gacaatatgt atagctatct
    17401 ggcgctggtg caggacgatc cgaccgtgca agtcgtgaac aacgcgcaaa aagcgtatgt
    17461 cgaacattac attcagggcg atccggatct ggcaaaactg ccggtgcttt cagctgccgc
    17521 accgtttaaa gttggtggtc gcaaaaatga cccggcaagc tatgtggagg tggaaaaagg
    17581 ccagttgacc ttccgtaatg ccgccgatct ttatctctac cccaatacgc tgattgtagt
    17641 gaaagccagc ggtaaagagg tgaaagagtg gctggagtgc tccgccggac agtttaacca
    17701 gattgctcct aacagcacga aaccacagtc actcatcaac tgggatggtt tccgcactta
    17761 taactttgat gttattgatg gtgtgaatta tcagattgat gttacccaac ccgcccgcta
    17821 tgacggcgag tgccagatga ttaatgccaa tgcagagcgg attaagaacc tgacctttaa
    17881 tggcaagccg attgatccga acgctatgtt cctggttgcc accaataact atcgcgctta
    17941 cggcggcaaa tttgccggta cgggcgacag ccatatcgct tttgcttcac cggatgagaa
    18001 ccgctcggtg ctggcagcgt ggattgctga tgagtcgaaa cgtgcggggg aaattcatcc
    18061 ggcggcagat aacaactggc gtttagcacc gatagctgcc gataagaaac tggatatccg
    18121 tttcgaaact tccccgtcag ataaagccgc agcgtttatt aaagagaaag ggcagtatcc
    18181 gatgaataaa gtcgcgaccg atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa
    18241 gtaaaacact tctttttcgg cctataaatc aaccgcatcc ggcatttatt ggcggatgcg
    18301 atgctgccgt atcttatcca ccctacaagc catgaaccgt agaccggata agcgcagcgc
    18361 atccggcagt tatgccgcac gttcatcccg caccgccaac acctcgggca aattcaactc
    18421 aatccagtcc gccagtgcag caaccttttc gctcacctgc tcgcccagcg gcgtgaggct
    18481 atattccaca tgcggcggca ccaccggata cgcgatacgg ttaagaaaac catcctgttc
    18541 taacgcctgt aacgactgcg caagcatctt ttcactcacc ccaccgattt tgcgccgcag
    18601 gtcgctaaag cgatgagtac cttcgcgtag cgccaccaga atcaacaccc cccaacggct
    18661 ggtgacgtgt ttcaacacct cgcgcgacgg gcactgttcc gcaaagagat taccctcttt
    18721 cagttgttgt gacaggctaa cctgactcat ttcatactta cctttttgca cgtacttact
    18781 aaaagtaagt ttaggtgtta gcgtatttaa acacaagaca aaacgatgga gacttcccat
    18841 gatcgctatt actggtgcca ctggccaact tggtcactat attattgaat ccttgatgaa
    18901 aacggttcct gccagccaaa tagtggctat cgttcgtaat ccggcaaaag cccaggccct
    18961 ggcagcacaa ggcattaccg tgcgtcaggc tgactacagc gatgaagccg cactgacatc
    19021 tgcacttcag ggagtggaaa aactactgct gatctcttcc agcgaagtgg gtcaacgtgc
    19081 cccgcagcat cgtaatgtta ttaatgccgc aaaggcggct ggcgtgaaat ttatcgctta
    19141 taccagcctg ctacatgcag atacctcccc gctcggcctc gccgatgagc acatcgagac
    19201 ggagaaaatg ctggctgatt ctggcatcgt ttacaccctg ctgcgcaacg gctggtacag
    19261 cgaaaactac ctcgccagcg ccccggcagc actggaacac ggcgtattta tcggtgcggc
    19321 tggcgatggc aaaatcgcct cagcaacgcg ggcagattat gcgacagctg cggcacgcgt
    19381 gattagcgaa gccggtcacg aaggcaaggt ttacgaactg gcgggcgata gtgcctggac
    19441 gttgacacag ttagcggcag agctgaccaa acagagcggc aaacaggtta cctatcaaaa
    19501 tctgagcgaa gccgatttcg ccgcggcact gaaaagcgtc ggactgcccg acggactggc
    19561 ggatatgctg gcggattctg acgttggcgc atcgaaaggt ggtctgtttg ataacagcaa
    19621 aacgcttagc aaattgattg gctgcccaac gacaacgtta gccgaaagcg taagccatct
    19681 ttttaatgtt aataactagt taattaaagt ggcatcctcc cgcatcctct ctgataatga
    19741 cgggatgccg ggagcaatca tgtctgcttc ctgaacgttt cttctgacag accaatggat
    19801 gccagtaatg attagcggcg tgctgtacgc cctgttagca gggttgatgt gggggcttat
    19861 ttttgtcggg ccgttgatcg tgccggaata cccggcgatg ttgcagtcga tggggcgtta
    19921 tctggcgtta gggttaattg cgctacccat tgcctggctg ggacgcgtgc gtctgcgtca
    19981 gttggcgcgt cgggactggc ttaccgcctt gatgctcact atgatgggca acctcattta
    20041 ttacttctgc cttgccagtg ccattcaacg tactggcgcg cctgtttcca cgatgattat
    20101 cggcaccctg ccggtggtga ttcctgtctt tgccaatctg ctttatagcc agcgcgacgg
    20161 caaactcgcg tggggaaaac tcgccccggc actgatttgt attggcatcg gcctggcgag
    20221 tgtgaatatt gctgagttaa accacggact ccccgatttt gactgggcac gttatacctc
    20281 tggcatcgtg ctagcgttag tttccgtggt ctgctgggca tggtatgccc tgcgcaacgc
    20341 ccgctggctg cgggaaaatc ccgacaaaca tccgatgatg tgggcgacgg cgcaggcgct
    20401 ggtcacgctg ccggtttctc tcatcggcta tctcgtcgcc tgttactggc tgaatataca
    20461 aacgccggac ttctccttac cttttggccc ccgtccgctg gtgtttatta gtctgatggt
    20521 tgcgatagcc gtgctttgct catgggttgg cgcactctgc tggaacgtcg ccagccagcg
    20581 attaccgaca gtgattctcg ggccgctgat tgttttcgaa acgctggcag gtttgctgta
    20641 caccttttta ctccgccagc aaatgccgcc gctaatgacg ctgagcggta tcgcgctgtt
    20701 agtgattggc gtggtcatcg cggtcagagc aaaaccagaa aaacctttaa ctgaatctgt
    20761 ctcagaaagt tgacatgctg gcagtgagtt aaataagcct ctgctacgta agggttatag
    20821 cttttgcctt aaaggtgtat ttaaaataca tcttatctta ttaagaatga ggtatcagct
    20881 atggcttatc gcgaccaacc tttaggtgaa ctggcgctct ctatccctcg cgcttcggct
    20941 ctgtttcgta aatatgatat ggattactgc tgtggcggta agcagacgct ggcgcgcgcg
    21001 gcggcacgta aagaactgga tgttgaggtc attgaagctg aactggcaaa gcttgctgaa
    21061 caaccgattg agaaagactg gcgtagcgcc ccgctggcag aaatcattga ccatatcatc
    21121 gtgcgctacc acgatcgtca ccgcgagcag ctgccggagc tgatcctgca agcgactaaa
    21181 gtcgagcgcg ttcacgccga caaaccgagc gtgccaaaag ggctgacaaa atacctgacc
    21241 atgctgcatg aagagctttc cagccacatg atgaaagaag agcagatcct cttcccgatg
    21301 atcaaacaag gcatgggcag ccaggcaatg gggccaatca gcgtaatgga aagcgagcac
    21361 gatgaagcgg gcgaactgct ggaagtgatc aaacacacta ccaataacgt cacaccgccg
    21421 ccagaagcct gcaccacctg gaaagcgatg tataacggca ttaatgaact gattgatgac
    21481 ctgatggatc acatcagtct ggaaaacaat gtactgttcc cacgcgccct agcgggtgag
    21541 tgatctaaaa gctggatggc attgcgccat ccagcatgat aatgcggtta tttccgcagt
    21601 tcagcagccc gcttcttacc aataaacagc cagcccaacc ccagcgcgat aaaccacagc
    21661 ggggtaacca gcagcgcctg gcgagtgtca tcttccagtg tcagcaacac gaccacgaac
    21721 acaaagaacg ccatacatac ccagcacatc agcttgccga gcggcatctt gtagatcgac
    21781 ttttcatgca gatgaggacg ctgtttgcgg tacaccaggt aagagcaaag gataatcgtc
    21841 cagacgaaca taaacagaat cgcggaaacg gttgtaatca tcgtgaacgc gccaatcacg
    21901 ctaggattca catacaacat caccacgccg ccgagcagac agatacacga gaaggtcaac
    21961 cctttcgcgg gtactgcgcg cttagaaagt ttagcgaacg ctttcggcgc aacaccttcc
    22021 tgcgccagac caaacagcat acggctggta gagaagacac cgctgttagc ggaagacgcc
    22081 gcagaggtca gcaccacaaa gttgatcacg ctggcagccg caggcaaacc taccaacaca
    22141 aacagctcaa cgaacgggct tttctccggg actaccgaac tccacggtgt cacggacata
    22201 atcacaatca gcgcgaagac gtagaacata atgatacgga tcggaatgga gttaatcgcg
    22261 cgtggcagtg atttctccgg atctttggtt tccgcagctg ttgtacctac cagctcaatc
    22321 cccacgaaag cgaaaaccgc tatctggaat ccggcaaaga agccacttaa acctttcggg
    22381 aaccagccgc cgtcattcca caaatgcgcg aatgacgctt ccacaccggt cggtgactga
    22441 aagtgcatcg ccaccatgac caggccgacg acaatcagcg acacgatggc gacgattttg
    22501 atcatcgcaa accagaactc catctcaccg aacattttca cggtggcaag gttgagcgtc
    22561 agcagcagca ctatcaccgc cagcgaggcg acccagtcgg agagatcggg gaaccagaac
    22621 tgagcataag ccgtgatcgc caccacgtct gccataccgg ttacaaccca gcagaaccag
    22681 taagtccagc cggtgaaata tcccgcccac ggccccagta aatcggaagc gaagtcacta
    22741 aaagatttgt attccagatt cgaaagcagc aattccccca ttgcccgcat cacgaaaaag
    22801 agcataaaac caatgatcat ataaacgaaa atgatcgacg gcccggcaag gctaatcgtt
    22861 ttgccggacc ccataaacaa cccagtacca atggcacctc caatggcaat aagctgaaca
    22921 tgtcggtttg tgagattgcg ccgtagcgac tgttcagccg gagcctgatc atcggcaacg
    22981 acttttacct gatctaccat gttttttctt cctgtacctg tctgtgttgt tcaggctcta
    23041 cggcctttag tcagtctatg atacgacgaa tctgtatcgg gactcatcga tattaggtaa
    23101 caatacgcgg gatgaatact aagatttaga tctaatgtta attttatgtt taaagtgagt
    23161 gtcatatcac ggtgataacg cgaaacagct cacaaaaata cacgcttacg gggtctttgc
    23221 aacacaaaat agcgccgttt ggcaaactga agggtttatt gctgaatgcc tgctcccctc
    23281 tggttcgagg ggaacagaat gcgctgctta gaggatttcc agcagttcga cttcaaacac
    23341 cagggtgctg aacggaggga tggatgcacc tgcgccgcgc tcgccatatg ccagttcctg
    23401 cgggatagtc agttcccatt tagaacctac cggcatcaga gtcagtgctt caatccagcc
    23461 agggatcacg ccattaaccg ggaattcagc gggttcacca cgagcaacgg agctgtcaaa
    23521 cacggtgccg tcgatcagtt taccggtgta atgaacacga acgcggtcgg tacgtgccgg
    23581 aattgcgcct tcaccctggt tgatcacgcg gaattgcagg ccagattcgg tgctattcac
    23641 accttctttt ttggcgtttt cttccaggta tttcacgcct tcagcagcca tcgcctggaa
    23701 acgctgacga cgaacggcat cggcgcgttc gtggatttca cgcagcgcgc gatgcaccac
    23761 atcaaccggc acagccggat gtttgccttc cagcgcatcg gcaatacctg caaccagtgc
    23821 ttctggcagc agcccttcca ggccagattc actcagttgt tgccctacct gcaaaccaat
    23881 gccgtagctt gcttgcgctt cgatggtgtc aaaagttggg gtggtcatcg tatttccttt
    23941 cttagggtat aaaagtagcg ggcagcatat cagccacact tctttgggta aaactttgtc
    24001 gcaggaatga tgacaaatcc cggggaaaaa gaaacaatag caatatcccg tatgaatata
    24061 ataaaggcgg gtttaccacg gacatcatag cgttagctta ttcataggct atgattgagg
    24121 aacaagacgt ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa ccaaccctgg
    24181 agaaagtctg gcacgcgccg gacaattttc gcttcatgga cccgctgccg ccgatgcatc
    24241 gtcgggggat cattattgcc gccattgtat tggtggtcgg atttctgctt ccatctgatg
    24301 atacgcccaa cgcgtcggtg gtgacgcgtg aagcgcagtt agatattcag tcgcaatcac
    24361 aaccgccgac agaagaacag ccgcaaacta cccaaacgca accattccag ccagatagcg
    24421 gaattgataa tcaatggcgc tcttaccgtg tcgaacccgg taaaacgatg gcgcagctgt
    24481 tccgcgacca cggactgccc gctaccgatg tctatgcaat ggcgcaggta gaaggcgcgg
    24541 gtaagccgct gagtaatttg caaaatgggc agatggtgaa aatccgccag aacgccagcg
    24601 gcgtggtgac gggcttaacc attgatacgg ggaataatca acaggtcctg tttacccgcc
    24661 agccggatgg cagttttatt cgtgcgcggt agatgaaggg tcaaccaaaa gccagctaag
    24721 gattgtctct ttgatgacct tacgtctggc ttccagtgcc tggggggaaa ccatactgat
    24781 atgataaact aggccaagcg tatgctggtt gatcaaataa tatcccccaa gcgcagcaat
    24841 attaatattc acctgcaatg ggtcgatacc tggcttaaaa atattgtatt tcttcccttc
    24901 atccaataag gattccatta actgcaagtg agcatggtta atttctaaga gacgctgtga
    24961 ttttgcataa tgcacacctt tactttgatt ttcactatgc acaattttta aaaaccaagg
    25021 attagcaata taataatccc atgtaaaatc aataattttc tcaatggctt tttgtggcgg
    25081 aagatctgag aaatcaaggg ccttttcttt ttgtcgaata tcgttccagg tatattccag
    25141 cacttcgata aataaatttt ctttattacc aaagtggtga taaaccgtct gtttactaca
    25201 ccctgctgcc tttacaatat tatctactct ggccccttca taaccatatt cggcaaattc
    25261 attaaccgca catagcagca atttttcctt caggctctgt acgttgctgg tatcaatata
    25321 cataacgctt ccccacgacc tcttcgtggt gactatcata acctgatttg atttcagctt
    25381 cagaaaaaag ggcaaaaact tgtactttca tcaggttatc aaatgtcctg tgatcgccat
    25441 cgcatatcaa ccaactagat ggtttatctt tttatcctcc agtcgcaagc taatcatcac
    25501 caaggaaaca aaattcaaaa aagagagcta ttgtatgaaa tccactcgtc ctgttgccgt
    25561 gattaccgga gccactcgcg gtattggaaa gggctgtgcg caggagcttg ctcgtggcgg
    25621 gtttaacctg ctgattaacg atcgtcccga tgccgacagc gtagaaaaac tgcatatcac
    25681 acaacaagaa tgtttggcgg aaggcgtgga agtgatttgc ttccctgctg atgtcggtga
    25741 tctatctctg catgaggaga tgctcgacgc ggcgcaaaac cagtgggggc ggctggactg
    25801 tctgctcaat aacgcgggga tttcagtgaa aaaacgcggt gacctcctcg acctcgaacc
    25861 agacagcttc gatcaaaaca ttgccattaa cacccgcgcg ccgttcttcc ttgctcaggc
    25921 attcagtaag cgcttactgg cacagccgaa accggaagcg gagctgccgc accgctcgat
    25981 tattttcgtc agttccatca acgccatcat gctggcgatg aatcgcggcg aatacaccat
    26041 tgccaaaacc gccgtttctg ccgctgctcg tctgtttgct gctcgccttt gtaatgaaca
    26101 aattggcgtt tatgaagtgc gccctggtct catcaaaact gatatgacta ttccggcaac
    26161 ggcttattac gacgaactga ttgccaaagg tctggtgccg tggggacgtt ggggttatcc
    26221 ggctgatatt gcttccaccg tccgcgcgat ggcggaaggt aaactgattt acacctgtgg
    26281 tcaggcggtc gcaatcgacg gcggcttaag tatgccgcgc ttctgaggct gaatcatgag
    26341 agatctgtta caacgcccgg atttattcag tatcaatacc gcgacactgg gctataaaac
    26401 gccgctaccc gccattattg atgcctgcgc cgcacgcggt attggcgcaa ttgccccctg
    26461 gcgcagggag ttgcagggtg aagatctcca gcaaatcacc cgccagcttg ccgccagtaa
    26521 catgagcgtt tccggtttgt gccgtagcac ttactacacc gcgccgacac tggcggaacg
    26581 caaactggcg attgatgata accgccgggc gctggacgac gcagcggtgc ttaacgccgc
    26641 ctgttatatg caggtggttg gcggtctgcc aacgggcact aaagatctat atgaagcgcg
    26701 agaacaagtg aaacaaggga ttcgccagtt gctgccacat tcgaaagatg tcggcgtgcc
    26761 aattgcgctg gagccactgc acccgatgac agctgccgat cgctcttgcc tgtgcacgtt
    26821 acgtcaggcg ctggactggt gtgatgaact cgatcccgat ggtgagtttg gcctcggcgt
    26881 ggcggtggat gtttatcacg tctggtggga tccggatctc gccagtcaga tcctgcgtgc
    26941 cggaaaacgc atcctcgcgt ttcatgtttc cgac
//
LOCUS       DAJTRN010000054        26271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRN010000054 DAJTRN010000000
VERSION     DAJTRN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            53..346
                     /locus_tag="OO328_002309"
     CDS             53..346
                     /locus_tag="OO328_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5930857.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            418..1077
                     /locus_tag="OO328_002310"
     CDS             418..1077
                     /locus_tag="OO328_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5930858.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1169..1615
                     /locus_tag="OO328_002311"
     CDS             1169..1615
                     /locus_tag="OO328_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5930859.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1822..2722)
                     /gene="hlyE"
                     /locus_tag="OO328_002312"
                     /pseudo
     CDS             complement(1822..2722)
                     /gene="hlyE"
                     /locus_tag="OO328_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3095..3514
                     /locus_tag="OO328_002313"
     CDS             3095..3514
                     /locus_tag="OO328_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5930860.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3514..4782
                     /gene="umuC"
                     /locus_tag="OO328_002314"
     CDS             3514..4782
                     /gene="umuC"
                     /locus_tag="OO328_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5930861.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(4828..5358)
                     /gene="dsbB"
                     /locus_tag="OO328_002315"
     CDS             complement(4828..5358)
                     /gene="dsbB"
                     /locus_tag="OO328_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5930862.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5504..7045)
                     /gene="nhaB"
                     /locus_tag="OO328_002316"
     CDS             complement(5504..7045)
                     /gene="nhaB"
                     /locus_tag="OO328_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5930863.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7266..7985
                     /gene="fadR"
                     /locus_tag="OO328_002317"
     CDS             7266..7985
                     /gene="fadR"
                     /locus_tag="OO328_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5930864.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8037..9569)
                     /locus_tag="OO328_002318"
     CDS             complement(8037..9569)
                     /locus_tag="OO328_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5930865.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            9899..11197
                     /gene="dadA"
                     /locus_tag="OO328_002319"
     CDS             9899..11197
                     /gene="dadA"
                     /locus_tag="OO328_002319"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5930866.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11207..12277
                     /gene="dadX"
                     /locus_tag="OO328_002320"
     CDS             11207..12277
                     /gene="dadX"
                     /locus_tag="OO328_002320"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5930867.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12445..13859)
                     /locus_tag="OO328_002321"
                     /pseudo
     CDS             complement(12445..13859)
                     /locus_tag="OO328_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14470..16206)
                     /locus_tag="OO328_002322"
     CDS             complement(14470..16206)
                     /locus_tag="OO328_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5930868.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16301..17215)
                     /gene="ldcA"
                     /locus_tag="OO328_002323"
     CDS             complement(16301..17215)
                     /gene="ldcA"
                     /locus_tag="OO328_002323"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5930869.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17315..17926
                     /gene="emtA"
                     /locus_tag="OO328_002324"
     CDS             17315..17926
                     /gene="emtA"
                     /locus_tag="OO328_002324"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5930870.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(17928..18662)
                     /locus_tag="OO328_002325"
     CDS             complement(17928..18662)
                     /locus_tag="OO328_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5930871.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            18863..19117
                     /locus_tag="OO328_002326"
     CDS             18863..19117
                     /locus_tag="OO328_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5930872.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19167..20627)
                     /locus_tag="OO328_002327"
     CDS             complement(19167..20627)
                     /locus_tag="OO328_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5930873.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20564..21121)
                     /locus_tag="OO328_002328"
     CDS             complement(20564..21121)
                     /locus_tag="OO328_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5930874.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21147..22001)
                     /locus_tag="OO328_002329"
     CDS             complement(21147..22001)
                     /locus_tag="OO328_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5930875.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22001..22810)
                     /locus_tag="OO328_002330"
     CDS             complement(22001..22810)
                     /locus_tag="OO328_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5930876.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(22820..23578)
                     /locus_tag="OO328_002331"
     CDS             complement(22820..23578)
                     /locus_tag="OO328_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5930877.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23575..24555)
                     /locus_tag="OO328_002332"
     CDS             complement(23575..24555)
                     /locus_tag="OO328_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5930878.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24555..25577)
                     /locus_tag="OO328_002333"
     CDS             complement(24555..25577)
                     /locus_tag="OO328_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5930879.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            25927..>26271
                     /locus_tag="OO328_002334"
     CDS             25927..>26271
                     /locus_tag="OO328_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930880.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNILH"
BASE COUNT         6602 a         6406 c         6781 g         6482 t
ORIGIN      
        1 gtttcattga tgttagctta tgccaaaacc tggcatctta aagagtaaaa gtatgttttg
       61 tgcgatttat cgaagcagca agcgtgacca gacctattta tatgtcgaaa aaaaagacga
      121 tttttcgcgt gttcctgagg aactgatgaa aggttttggt cagcctcagt tagcgatgat
      181 tctgccgttg gatgggcgga agaaactggt caatgctgat attgaaaagg ttaaactggc
      241 attaaccgag caaggttact atttgcagtt accgccacca cccgaagatt tgctgaagca
      301 acatctttcc gtcatggggc agaaaacaga cgacactaac aaataaccga tatccggcgg
      361 tggcattatc tttgtcggcg cgggttttca tatccacgat aaggtgaggg gaacgttatg
      421 tatcaacatc acaactggca aggtgcgctg ctggattatc cggtgagtaa agtagtctgt
      481 gttggcagta actatgccaa acatattaaa gagatgggca gcgcagtgcc cgaagagcca
      541 gtgctgttta ttaaaccaga aacggcactg tgcgatctgc ggcagccatt ggcgatccca
      601 tccgatttcg gttcagttca tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg
      661 cgtcaggcaa cggaagagca tgtccgcaaa gccattgccg gttacggcgt ggcgcttgat
      721 ctgacattgc gtgatgttca gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggct
      781 aaagcgtttg ataactcttg tccgctttcc gggtttatcc ccgcggcgga atttaccggc
      841 gatccacaaa atacaacgct gggcctgagc gtaaacggcg aaaaacgcca gcaaggtacg
      901 actgcggaca tgatccataa aatcgttccg ttgatcgctt atatgagcaa gttttttacc
      961 ctcaaggccg gtgacgttgt gttgacaggc acgcctgatg gcgtcggccc gttgcaaagc
     1021 ggtgatgagc tgacagtcac tttcgatggg cattctttga caactcgcgt tttgtaatac
     1081 tttttgccgc cagaaagcgg cggcaaaact tgcatcgctg tgccagactg gttataaggt
     1141 gcgttttaac gtaatggcgg aacacctgat gagcgatgta cctttctggc aaagtaaaac
     1201 cctggacgaa atgagcgatg cggaatggga gtcgttgtgt gatggttgcg gtcagtgttg
     1261 cttgcataaa ctgatggatg aagacaccga cgaaatctac ttcactaacg tcgcctgtcg
     1321 ccagctcaat attaaaacct gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc
     1381 cgactgcatt aaattaaccc gtgaaaatct gccaacattc gaatggctgc caatgacctg
     1441 cgcttatcgg ttgctggcgg aaggtaaaga tttacctgcg tggcatccgc tacttactgg
     1501 ttcgaaagcg gcaatgcatg gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc
     1561 agaagtgatt gactggcagg atcatatctt aaataaacct gactgggcgc agtgataaat
     1621 ttaatagatg ctgatggggg ctttcttaat atacgtctgg atgctcgcca tttcgtagcc
     1681 ctgtttttga aagcgctctg tcataatgag agttacccga taccttatta tcaacaggta
     1741 actctcaatc tttaaaaaac gtctgtgttt ttaaatgtga taaaccttat accttgagta
     1801 catggagaga ataatcgctt atcagacttc aggtacttca aagagtgtct ttttaccgtg
     1861 tcttttctga tactcattac aggtgttaat catttttttg gccgctgctt ttagcaaaga
     1921 aagcattaaa tcatcataat caacatagaa tctggttgtt tcagtttccg tttttatctc
     1981 cccgatggcg gctatttcgg tggttaattt caatttggcg gcatcgatat ctttattcgc
     2041 ttgtttaacc gtgttagaca gggtggtaaa gaaactctgc acagatttta acttgttctt
     2101 caattctgga atcagtttcc cttcaactac gcccgcagca atagaatagg aaatgattaa
     2161 accaaatgga ccggcgacga catcggctgc ggcaccggca tacgcttcct tcctgatttt
     2221 atctacctgt gactggaaat agctgctttt ttctgaaaaa tcattggtta actggctatc
     2281 taacgccagc agtttcccgg aagcgttgtt gaaactttgt gagcttacca gcagggattt
     2341 ttgcgcttca ttcagcttcg tgatgccgtc atccagtacc ttaatgaggg gcggatgctt
     2401 tcttctcatt gtactcatca aatagcaaaa tatacgctgc gagcaattgc gtcgcaacac
     2461 cacaccattc atacactgtt tgggttgctt caaaatactt atcctggcta tccataagta
     2521 aggttttaat atcgccgact aaaacggagg ctgcctgtga atactcctgt ttaaagcgac
     2581 ttaactcttt tatggtttca tcaaaggtct gccaggggat gacctgatcg agatatttat
     2641 tataaagatc taatgctcca tctgcggttt cgattgcgtt tttaactact tctaccgttt
     2701 tatctgcaac gatttcagtc ataatcattc gcctctttaa atatataaat tgtaatgaaa
     2761 ctcctgtttt acaactatta ataaatttta cttcatctaa ttcatagtta gccgggcggg
     2821 atgcgtcaat gtctttattt ctattaatat gataaatatc aaacaatgtt taatgtcatt
     2881 atggcgaatg cttctattct atttttttgc cgggtgatat ttttcatttc tgctggatga
     2941 gcgtcgtcgc cagaaggcca cgtgagcaca ggataagaga acgaaaaatc agcagtctat
     3001 gcagcgacaa atattgatag cctgaatcag tattgatctg ctggcaagaa cagaccactg
     3061 tatataaaaa cagtataact tcaggcagat tattatgttg tttatcaagc ctgcggatct
     3121 ccgcgaaatt gtgacttttc cgctatttag cgatcttgtt cagtgtggct ttccttcacc
     3181 ggcagcagat tacgttgaac agcgcatcga tctgaatcaa ctgttgatcc agcatcccag
     3241 cgcgacttac ttcgtcaaag caagtggtga ttctatgatt gatggtggaa ttagtgacgg
     3301 tgatttactg attgtcgata gtgctattac cgccagccat ggtgatattg tcatcgctgc
     3361 tgttgacggc gagtttacgg tgaaaaaatt gcaactacgc ccgacggtac agcttattcc
     3421 catgaacagt gcgtactcgc ccattaccat cagtagcgaa gatacgctgg atgtctttgg
     3481 tgtggtgatc cacgtcgtta aggcgatgcg ctgatgtttg ccctctgtga tgtaaacgcg
     3541 ttttatgcca gctgtgagac ggtgtttcgc cctgatttat ggggtaaacc ggtggttgtg
     3601 ctatcgaata atgacggttg cgttatcgcc cgaaacgctg aggcaaaggc gctgggcgtt
     3661 aaaatgggcg atccctggtt caaacaaaaa gatctgtttc gtcgctgtgg tgtggtttgc
     3721 tttagcagca attatgagct ttacgcagac atgagcaatc gggtgatgtc gacgctggaa
     3781 gaactatcgc cccgcgtcga gatttacagt attgatgaag cattctgcga tctgacaggt
     3841 gtgcgtaatt gtcgcgatct gactgatttt ggcagagaaa ttcgcgcaac ggtgctacaa
     3901 cgtacccatc ttactgttgg tgtggggatc gcccagacca aaacgttggc taagcttgcc
     3961 aatcatgcgg caaaaaaatg gcagcggcag acgggtgggg tggtggattt atcaaatctg
     4021 gaacgccagc gtaaattaat gtctgctctc cccgtggatg acgtctgggg gattggacgg
     4081 cggatcagca aaaaactgga cgcgatgggg atcaaaaccg ttctcgattt ggcggataca
     4141 gatatccggt ttatccgtaa acattttaat gtcatgctcg aaagaacggt gcgtgaactg
     4201 cgcggcgaac cctgtttgca actggaagag tttgcaccga cgaagcagga aattatctgt
     4261 tcccgctcgt ttggtgaacg catcacggat tatacgtcga tgcggcaggc catttgtagt
     4321 tacgctgccc gggcggcgga aaaacttcgc agtgagcatc aatattgtcg gtttatctcc
     4381 acatttatta agacgtcacc atttgcgctc aatgaacctt attacggcaa tagcgcgtcg
     4441 gtaaaactgc tgacgcccac tcaggacagc agggatatca ttaacgctgc tacgcgatct
     4501 ctggatgcca tctggcaagc gggccatcgc taccaaaaag cgggcgtgat gctgggggat
     4561 ttcttcagtc agggagtcgc gcagctcaat ttattcgatg acaacgcacc gcgccccggg
     4621 agtgagcaat tgatggcggt aatggatact ctgaatgcta aagagggcag aggaacactc
     4681 tattttgccg ggcaggggat ccaacaacaa tggcagatga agcgagctat gctttcacca
     4741 cgttatacaa cgcgaagttc tgatttactg cgggtcaaat aaatatagcg gcaggaaaaa
     4801 agcgctcccg caggagcgcc gaatggatta gcgaccgaac agatcacgtt ttttcgcttt
     4861 aaacggctgg gaaatcacca ccagcaccgc gacaatcagg taagcgataa aaataccgag
     4921 cagccactgc ggcatttcca tacctaaaaa atcccactga cgctcggcgc aatcgccaga
     4981 ggcgacaaac acttgcggca cccatttatc cagcggcagc cattccggga aacgaaccat
     5041 aaaatcacag gtggcaaatg gcgaaggata gagctgaagc atggtgtgct cgtaagttaa
     5101 ctgcacaccg cggaacgcac tatacaacca gataaccatc gctacataac gcagcggagt
     5161 tttcggggcg atcgcgccaa tcagcgcagc acccagaacg ccgaataacg cgcagcgttc
     5221 ataaatacag agcacgcaag gtttcagtaa catcacatgc tggaaccaca gcgccgtcag
     5281 ttccagtgcc agagcagtaa acgccatcaa cagccacgcg ccccggcctt gtgaacattg
     5341 gttcaaaaat cgcaacataa tcatttccct gcaatatgca tagagtgcgc agtttaaacc
     5401 aattcattcg ctgcgccacc aggaggcagc aaaaatagga cgcaattgga caattatcgg
     5461 gcgaaaaggc aaaccgggca tttatatgcc cggtaagttg ttatcaaagc gttgctatcc
     5521 agcccatttg cataaaccat tcggttacag gggcaagcgt aaactcgacg cagagcaagc
     5581 cgacgagtgt cagtacgagg gtgtaaggca gggccatcca caccatgcgg ccataagaga
     5641 ggcgaatcaa tggcgcgagt gcagaggtca gcaggaacag gaacgcagcc tgaccgttcg
     5701 gcgtagcgac agagggcaga ttggtaccgg tattaatggc gaccgccagc agctcgtatt
     5761 gcttcaacgt gatagcgcca ctttccattg ccgctttcgc ttcgttgata taaatcgtcc
     5821 ccacgaagac gttatccgaa atggatgaca gcagaccgtt gaaaatatag aacagcgaca
     5881 gctgagcatg ttcagatgcc tgcaacacaa actgaataat cggcgaaaac agttgttggt
     5941 cgataatcac cgcgactacc gagaaaaaca ccgtcaacag tgcggtgaat ggcagagatt
     6001 cggtgaaggc tttaccgata gcatgttcat cggtgacacc ggtcaatgat gttgccagaa
     6061 taatgactga caaaccaatc aagccaactt ccgccagatg caacgccagc gcagtcacca
     6121 gccagacgcc aataatcgcc tggacaatca gacggatttt atcctgacgg gtacgctggt
     6181 ggcggctttg atcgtcaaac tgttgcaaca cttcgcggac tttctccggc agcgtttcac
     6241 cgtagccaaa ccaacgcagc ttctctacca gcaggcaggt taacaggcca caaatcagaa
     6301 ccggaacggt caccggcgac atgcgcagga agaaatcgcc aaaatgccag ccagccgctt
     6361 tagcgatgat caggttctgt ggttcgccca ccatggtcat tacgccgcct aatgcggtgc
     6421 cgacaccggc atgcatcatc aggctacgca gaaagccacg gaactgttcc agaaccacgt
     6481 tgtaatgctt gtcgatatga ctatcgtctt gcaggtcggt gtcttcggta cgggaagagg
     6541 ctacgcgatg ataaatacca taaaaaccga ctgcaacgct gatcaccacc gccacgacgg
     6601 ttaaggcatc gaggaacgcg gagaggaacg ctgccgccac gcaaaaagag agcgacagca
     6661 gcattttgga gcgaatgctt aacagcaaac gggtaaatat gaacagcaac agctgtttca
     6721 taaaatagat acccgccacc ataaacatca ataacagcaa gacttcaaga tttgccgcca
     6781 cctcttcacg gacgtgttcc gcgctggtca tgccgatgaa taccgcttcg atagccaaca
     6841 gaccaccggg gagtagcggg tagcatttca gggccatcgc cagagtgaaa ataaattccg
     6901 ctaccagcaa ccagcccgcg acgaaagggc tgatgaggaa aattaacggg tttacgatta
     6961 agaaaatgat gagggcgagt ttgtaccagt cgggggactg gcccaaaaaa ttgcgccata
     7021 gcgcgcggcc ccaggagatc tccatgatgg tttcccttac cttacaaata atcaatgatg
     7081 tttttatgtt taaacgcaaa gcttaacggt caggcaggag tgaggcaagt cttgatagtc
     7141 aaggggaaag agatgcggaa aatgaagcct tgatcccttt ttcttctttt tgtctgctat
     7201 cagcgtagtt agccctctgg tatgatgagt ccaactttgt tttgctgtgt tatggaaatc
     7261 tcactatggt cattaaggcg caaagcccgg cgggtttcgc ggaagagtac attattgaaa
     7321 gtatctggaa taaccgcttc cctcccggga ctattttgcc cgcagaacgt gaactttcag
     7381 aattaattgg cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg gcacgagatg
     7441 gctggttgac cattcaacat ggcaagccga cgaaggtgaa taatttctgg gaaacttccg
     7501 gtttaaatat ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg cagcttattg
     7561 ataatttgct gtcggtgcgt accaatattt ccactatttt tattcgcacc gcgtttcgtc
     7621 agcatcccga taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg
     7681 atgcctttgc cgagctggat tacaacatat tccgcggcct ggcgtttgct tccggcaacc
     7741 cgatttacgg tctgatcctt aacgggatga aagggctgta tacgcgcatt ggtcgtcact
     7801 atttcgccaa tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt
     7861 tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga
     7921 gtggcgagat ttggcaccgg atgcagaaaa atctgccggg tgatttagcc attcaggggc
     7981 gataatccct tccgtttaaa gagcaacccc ctcaaacgag gggttttttg ttgtttttac
     8041 agatttccca ttcttggcgg gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc
     8101 tgttcgagca tcacatcaaa tccccacagg cgatgcacat gcttcaggac ttctttgcgc
     8161 ccccgatcca gcggtgcgcg attatgtgga atataacgca gcgtcagcga acggtcgccg
     8221 cgcaaatcca cgttccagat ctgaatattc ggctccagat tacttaagtt atattgcgac
     8281 gataaccggt tacggatctc ccgataacct tcttcattat gaatagcgga aatctccaga
     8341 taattatgcc gatcatcatc cagcacggtg aagaagcgga aatcacgcat cactttcggt
     8401 gacagaaact ggctgataaa actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt
     8461 tccagccagt cggaaccggc gatatccggg aaccagtatt tgtcttcttc cgttggcgac
     8521 tgacaaatcc gtttaatatc ctggaacatg gcgaacccga gggcatacgg gttgatgccg
     8581 ctgtaccacg ggctgttata ggggggctgg aagaccacat tggtgtggct gtgcaaaaac
     8641 tccagcataa aacgttccgt tactttccct tcatcataca gatggttaag gatggtatag
     8701 tgccagaagg tcgcacagcc ttcgttcatc acctgagtct gtttttgcgg ataaaaatac
     8761 tggctcacct tacgcacaat acgcaggatt tcacgctgcc atgattccag cagtggcgca
     8821 tttttctcca taaaatagag caggttttct tgtggttcgg acggatagcg gcgcgcttca
     8881 gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc agagcatatt gacctgactt
     8941 tgtagatact cttcgcgact tttctgccgg gctttctctt cttgcagcga gattttttgc
     9001 gggcgtttgt accggtccac gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt
     9061 tctacttcat caacgccata acgttcttcg cactcggtaa tatatttacg ggcaaaaatc
     9121 agataatcga caatcgaact ggcgtcggtc caactgcgga ataagcaatt gtttttaaag
     9181 aaagagttat gtccatagca ggcgtgtgcc atcaccagcg cctgcatagt aatggtgttc
     9241 tcttccatca agtaagcgat acacgggtta gagttaatga cgatttcata ggccagccct
     9301 tgctgaccgt gcttatacag ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc
     9361 ggatagttaa ttggcatgcc gacgctggaa taggcatcca tcatctgttc tgaggttatc
     9421 acttcaatct ggtgcgggta ggtatccagc cggtagagtt tcgccacccg gtctatctct
     9481 gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg
     9541 tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg
     9601 tgtggagcac ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc cggagaattt
     9661 ttttctttgc gatttcttat tatcagagtg ccactaatcc gcttctgaac ggaattttat
     9721 gctggataaa aagggcgttc agcaggagat actaaagacg ccatattgcc gcagagtcag
     9781 ggagatgtga gtcagctcac cataaaaaag ccgtatgttg aataatattt tcaactgagt
     9841 tatcaagatg taattagatt attattcttt tactgtatct accgttatcg gagtggctat
     9901 gcgagttgtc atactgggaa gtggtgtggt aggcgttgcc agcgcctggt acttaaatca
     9961 ggcaggacat gaggtcaccg tcattgatcg ggagccgggg gccgccctgg aaaccagtgc
    10021 cgcgaatgcc gggcaaatct cccccggata tgctgcaccg tgggcagcac caggtgtgcc
    10081 tttaaaagcg attaaatgga tgttccagcg ccatgcgccg ctggcggttc gtctcgacgg
    10141 tacgcagttt cagttgaaat ggatgtggca aatgttacgt aactgcgaca ccagccacta
    10201 catggaaaac aaagggcgga tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc
    10261 attgcgcgcc gaaaccaata ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt
    10321 ccgtaccgaa caacagtatg aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg
    10381 cgtaccgtat cagctgctgg aatccagccg cctggcggaa gtggatcccg cgctggcaga
    10441 agtagcgcac aaactgacgg gcggcctgca gttacccaat gatgaaaccg gagactgcca
    10501 gctgtttacc cagaatctgg cgcggatggc agagcaggcg ggggttaaat tccgctttaa
    10561 tacgcccgtt gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga agtttggcga
    10621 tgaagtgatt aaggccgatg cgtatgtgat ggcgtttggt tcttactcga cggcgatgct
    10681 caaaggcatt gttgatattc cggtgtatcc gctgaaaggc tactcgctga ctattccaat
    10741 tgcgcaggaa gatggtgcgc cagtatccac cattcttgat gaaacctaca aaatcgccat
    10801 tacccgtttc gataaccgca ttcgtgttgg cggaatggcg gaaattgttg gttttaacac
    10861 cgagctgttg caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg
    10921 cggcggtcat gtcgagcagg cgactttctg gacgggtttg cgcccgatga cgccagacgg
    10981 tacgccggtt gtcgggagta cacgctttaa aaatctgtgg ctgaataccg gtcacggcac
    11041 gctcggctgg acgatggctt gcggttccgg tcagttgtta agcgatctgc tctctggtcg
    11101 cacgccagcg atcccatatg aggatctaag cgtagcgcgc tacagccgtg gatttacgcc
    11161 atcacgtccg ggccatttac atggcgcaca cagctaagga aacgagatga cccgtccgat
    11221 acaggccagc ctcgatctgc aggcattaaa acagaatctg tccattgtcc gccaggccgc
    11281 gccgcacgcg cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg gtattgagcg
    11341 tatctggagc gcgctcgggg ccaccgatgg ctttgcatta cttaacctgg aagaggcaat
    11401 aacgttacgt gagcgcggct ggaaggggcc gatcctgatg ctggaaggat ttttccatgc
    11461 tcaggatctg gagatttatg accagcaccg cctgaccacc tgcgtacaca gcaactggca
    11521 gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg gatatttatc ttaaagtgaa
    11581 cagtgggatg aatcggttgg gcttccagtc cgatcgcgtg cttaccgtct ggcagcagtt
    11641 gcgggcaatg gcgaatgttg gcgaaatgac cctgatgtcg cattttgctg aagcggaaca
    11701 tcctgatgga atttccggcg cgatggcgcg tattgagcag gcggcggagg ggctggagtg
    11761 tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac ccggaagcgc attttgactg
    11821 ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc ggtcagtggc gtgatatcgc
    11881 caataccgga ttacgtccgg tgatgacgct aagcagtgag attattggtg tccagacgct
    11941 aaaagcgggc gagcgtgtgg gctacggcgg tcgctatact gcgcgcgatg aacagcgaat
    12001 cggcattgtc gccgcaggat acgccgacgg ttatccgcgc catgcgccta ccggtgctcc
    12061 tgttttagtg gacggcgtgc gcaccatgac ggtggggacc gtctcgatgg atatgctggc
    12121 ggttgattta acgccttgcc cgcaggcggg gattggtacg ccggttgagc tgtggggcaa
    12181 ggagatcaaa attgatgatg tcgccgccgc tgccggaacg gtgggctatg agttgatgtg
    12241 cgcactggcg ttacgcgtcc cggttgtgac agtgtaactt gttgtaagcc ggatcggagg
    12301 cagcgtcttc tggttgcgaa aaaatcatcc atccggctgg tcagcaactg tagttgttaa
    12361 tgtgacagag ccattgccca tgatagtgtc cattaaaaga atggacacta tttccccgga
    12421 acctgaactc gccgcacagg cgttctacat aaaacgctta cgcttcattg ttgactcgga
    12481 gtcgacttca gataaatcac gcttcaccct tgatgcagat tcgggttcat gttcagattg
    12541 ttgattaaca ggctgcgagt cctgttgtct ttccagactc aacaggccca actgttgatc
    12601 caggagctcc aggtcctcct gtaccttcac gcgctccagg agttccgggt catccaggaa
    12661 ctccagatcc ataaggtcct ccagctcctc gagtccttcc aggtcaacct agccttcctt
    12721 gtgcaaaaag tccaaaaggt ccttcaggcc ctgctgtttt ttcaggccct cgacggcttt
    12781 gagcagcgtc ggtaaattgt ctttgtccca cgggttacca ttacagtcaa acaccttcag
    12841 gctctggggc tgcggcacct gtaccgtttt aaacttgttt tctccacagt agtaaattat
    12901 tagtttgggc ggcagccgtg gtgaagagat caaatcgtta ctagaacagt caaatacttt
    12961 caggcgagtc aagttctcca gcatctcatc aggtattgag ttaatattgt tgccggcaca
    13021 ggatagttta tacaggtact ggggcatcga cggcggtaaa aattgcaaga agttttcaga
    13081 acagttaatc actctcaggt tagtcgacag ctcaggcagc cagataatac tgttttcata
    13141 gcacttcagt accatcagtt ttgagggctg caccttcggt aacaagatca agacgttatc
    13201 tgtacagtca agcaccagca gccactttgg catcaccttc ggtaacgcgg tcaacacgtt
    13261 tttactgcag taaaattcaa tcagatgtgg aggcaaattc tcagggagtg aagtcaatcc
    13321 caggttgcta agattgagca tactccgtct tgtaatgaga cattccttca ttctgcgcaa
    13381 tgcttctgcc cggttttcaa tccggcttgc tggcaggttt tcttccagga tattttcctt
    13441 gatttcttcc agactttctt tcaggtcttc ttggggaatt tctgactgtt tgttttgctg
    13501 gttttcttcc aggatttcgt acaggttttt ttgcaggttt tcttgcaaac catcctgctg
    13561 tatagcccat tcgcaccatt tggcgaccat catgaactcg ttatgttgct gattcagacg
    13621 agtccccaac gtgatgatgg tattgttgtc cacctgtatg ttcactttgc ccgcgatact
    13681 gaggtcaatg atctgccagg gggcatcacc ccagctgaat ttttgcgggc tgagatactt
    13741 catgttctgc aaaatttgat tcttgagaag agccgccctg gcgggcgtcg tacgcctggg
    13801 ttaacggaag atgggggaat ggcgggggcg aatgggcaga tgtctttaaa gctagccata
    13861 atgctacgtt tccttgaaat aaatatcctc tggcagcgcc ggagacgtga atgtggtcac
    13921 cgcaaaggag ccacaggacg tcatttgcgc tgatatcact actcatcgtt gacttttttc
    13981 ctggtcagaa atgccgtgtc gggtctggta catgctgtat tacggcctag gtagctctga
    14041 cgtattaaag aaaatacgag agtgaacgac ccggaaataa tcgttattaa gcaaaggaat
    14101 aactataaga aatcgctcat gcatccggtg ctacaggcgg atgaaacgcc tattgtgtgg
    14161 gctatgtcag tgcggcaggt tgcgtggggg aatatgttca ggcgatgtca ggcaggggcc
    14221 cctgatggtt gaccggtaat gcggggctgg tttttattac ctgttacagt cggaaaaagc
    14281 ccttacgctg actttcttga gccggtgttt tgagttcatt agccagagcc tgcaatggtt
    14341 tgtcatccat aatttaacct tcttctgatg ttgggatgag catatcaaaa tcaggaaatg
    14401 acaagtttat tacaggctca ggctgcgttc agtaaagcat cgcatccggc gatagatgct
    14461 gcattaaatt taagattcag cttcctcttc agccacccga acgccaatct tcaacacttc
    14521 attatcttct ttctcggcca ccgtccagat catcccggca aactctacct ggtcaccgac
    14581 aaccggtgcc gcgcctagca actgctggac aatttcaccc agcgtttgct gtttatcacg
    14641 atattctcgc ccgtcttcca ggccatatat cagcgcaaca tcagcatatt tggcgctggc
    14701 ttcgagaatg aagtcaccaa agaagcgttg atccagcgcg accggtggcg actggctgaa
    14761 cagtttaccg agcgccggga gatcgcgttc ccgaccaatt acacacaata catcgccttc
    14821 gcgcagtcgg gtgctgccgg taggatgaag caactggtta tcacgaaaca gggctgcaat
    14881 acgcgtctct tttggcatat gcaaatcgcg cagtgccgca cctacacacc atttatcagc
    14941 actcaattga taaacaaact gctcccacgg attttccgga tgaatatcca ggccaacgcg
    15001 tgacaccgga cgtccgacgg gcggaaccac cactttggct tttttcgccg cccacgagag
    15061 tgatgttccc tgcaagagca gtgaaaccag aaccacaaag aaggcgacat taaagaacag
    15121 tcgcgcattc tccagccccg ccatcatcgg gaacacggct aggatgattg gcaccgcgcc
    15181 gcgtaatccc acccagctga taaacacgcg ctcacgcaga ttgaaaccgc ggaaagggag
    15241 caatccagca aataccgaaa ggggacgggc gaagaatatc atccatgccg acaaaatgag
    15301 cgccggaatg gcaataggca gcagatcgct tgggttaacc aatagcccca gcaccaggaa
    15361 catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt
    15421 gcgaatcggc cgattaccca gcagaaaacc gcacagataa acagccagaa taccgctgcc
    15481 ttccagcgcc gtggttaaag caaaaattaa aataccgccg cttaatgcca gcaatggata
    15541 taatccggca ggcagggcga tgcgattaat catttgcagc agtaaataac caccgccaag
    15601 accgatgaca atcccgaggc caaattgctg cagaatatcg accacgaaca tccaactgac
    15661 actgctttca tgctgctgga tcattgcaat cagggtaatc gtcagaaaga ccgccattgg
    15721 atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa gccccttacc
    15781 acccagcaga gaaaagaccg ctgcagcatc ggtggagccg acgatagcgc cgattaataa
    15841 gccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta aaccagaggt
    15901 gataagtacg cccagcgtcg ccagcgacag cgccggtcct aacgccacac gaaaggaact
    15961 ggcctgagtg cgcatcccgc cgtcgagcaa aataatcgcc agcgccaggt tacttaccat
    16021 gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcgactc ccgccagcat
    16081 gccgatcgcc agaaaaataa ccagaatagg aatgccaaga cgggaagaaa atgaactaag
    16141 taatatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta ttgttgtggc
    16201 atccaatggt cggttactcc tgattacgct gtctcttata taaaccctac catattagcg
    16261 gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta agaacaggat
    16321 gaccgctaat cgtcaactga gcgccttccc gggtgttatt cagaatggca tgtgcgccca
    16381 acggcagcgt aaccgtgcgt tgttcatgac caaaatcgag gccggtaatg agtggaatcg
    16441 acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg tcataatcat
    16501 tgggcgtgct gccgctaaag ctaccgagaa taatcgcctt ctgacgtggc aaaattcccg
    16561 catgatagag ctgcaacagc atacgttcga cgcggaaagg gtgcacgtta atatcttcca
    16621 gcaccagaat accgttctca atttttggca tccacggtgt accaatcagt gaaatcagca
    16681 tcgcaagatt gcctccccac aacgtgcctt cggtctgaca tgtcgggcct tccccttgcc
    16741 attcaatggt gaaggtttca ttgcgtaacg ccagccagaa atggtgctcg gtaaaggcgt
    16801 tcagttcatc cgcaccaaag tttgctacca gcatggggcc gctaaaggtg atgacattgc
    16861 cttgcgccag aagaccgcac tgaatggcgg taaaatcgct atgtccgcaa atgagtaacg
    16921 ggtcatgttg ttggcgagcc accagtgcct gccagtcaat atctgccagt aaacgactgg
    16981 caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtagtcagt ctggcaaggg
    17041 aattgagatc ttccagacgc tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa
    17101 taacctcgac gttattgact tgatgccccg cgtcggtaag gcgctggata ccacgcagcg
    17161 cggcgtgctg tttaatgctg taacccgatg gggcaattaa gtgaaacaga gacatggcaa
    17221 ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc
    17281 tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt ttgccttttt
    17341 gattgtgtta ttagcgggtt gttcatcaaa gcatgactat acgaacccgc cgtggaacgc
    17401 gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag ccggtgcagc
    17461 ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg gtggtaatcc
    17521 gaacgcggtg agtaaatcga atgccattgg tttgatgcag ttaaaagctt caacctccgg
    17581 acgtgatgtt tatcgccgta tgggctggag tggtgagccg acgaccagcg agctgaaaaa
    17641 tccagagcgt aatatttcaa tgggggcggc ttacctgaat attctggaaa ccggcccgct
    17701 ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat acgctaacgg
    17761 ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa
    17821 cgatttggat gctgacgagt tcctcgatca cgtagcgcga aatcatcctg cgccgcaggc
    17881 tccgcgctat atctacaaac ttgagcaggc actggacgcg atgtaaatca gtcgcgcact
    17941 ttgtccgctt tttcccgggc ttctcgctcg agagagaaaa taatccgctg taattgccgc
    18001 tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt gatggtttca
    18061 ttcttgccat caatcacttt gcgctcgctg gtggagatta actgggcgtc aaagtgaaaa
    18121 acaccccatt gccccatgtt gacttcaatt tgagcgaagc gcataccttc ttgtaattcg
    18181 gcaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc atacaagcgg
    18241 aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg atggagtggg
    18301 gcggagatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg cggtacggta
    18361 ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt aaactcgact
    18421 ttcgcaccct gagtttcggc ggtaatggta atgtgctgtg cctttagcac ggcgatgttg
    18481 tcttcggctt gactgccgaa atccagcacc agtttatccg gggttattgc cagtattttg
    18541 ctgatcagct gcccgccatt ccaactgaga cgcaaaggaa ttgcggcttt gtgcaaatcg
    18601 cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg gtaatgactc
    18661 acgcggaaaa actcctgact gacaaactgt ctttggttta tcggtcatag ttaacaaaac
    18721 ttaatacaaa tgcgtgaata ttttttacat gttgttctta aatcagccga caaacgccca
    18781 gcatcgatcc cgcccttgcc tatacttaga gcgttgacgt aagcatttct tgcgaccgat
    18841 tcatcgaaac gagggcctga acatgggaat tattgcctgg attatttttg gcctgatagc
    18901 cggcattatc ggcaagctaa tcatgccggg gcgtgatggt ggtggatttt tcctgacctg
    18961 tattctcggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt ttggcattga
    19021 cggctccatc agcggtttta atttgcacag cttcctggtg gcggtggtgg gcgctatcct
    19081 ggtgctgggc gtattccgcc tcctgcgaag agaataagat tttcataagg cggatagcga
    19141 tacagatgcc gctatccgct ttcacatcag aacgtatact cgacacctgc ccagaaattg
    19201 cgaccttctt caataaaccc ttcactgtag gcatattggg tatcaaacag gttattgacc
    19261 gacgcattga cgctgaagcc atgacctaag gtgtaatcgg ctcgaatgtg ggtcaccgca
    19321 aaaccggcgg ctttttgtga accgtcactg ttgctgtagc tggaggaacg cgcctcttcc
    19381 gacagcgtta cgcttaacgg ctcccatggt ttgagtgtca tccatgcggt cattgtctgc
    19441 gttggaagat cggttatctt gccgatgtct ttgcgtttgg cgtcagcgtg gatcagagcg
    19501 tagctcaatc ctacatccag tatattgctg attttcccct taataccggc atccagaccg
    19561 ctgtaatcca ccgtgccgct gttctgattt tgaatggtat cggcattgat attgtgcgag
    19621 aggatggcat cactcacccg gttatagtaa acgctgacct caaatcccca gtcgcgcgtg
    19681 aaggcaccat tccaggttaa atccaccccg cgagcgcgtt ccggcttgag ctgcgggtta
    19741 acaatcgcta tctggttata cgcaggtttg gatgtggtat agcgttcttt cagcgtcgga
    19801 aagcgtgtgc ggtcatagta cgaaagcgcc agagtgtctt cattggcaaa gtggtatttc
    19861 cccatcacct gccagttaaa agctgactga ttgttgtcgt cataatgggt gatgctgcca
    19921 tctttctcgt gttttttcgc ttctacgcta tcgcgccagt catagctgat tcccgccacg
    19981 acatcgacat tatcagcagc agcccattga tattcactgg cgagcgacca ggtacgatct
    20041 tcatagcgat cgtaagcggc gtgcggcgca cctttttccc ggtgtacgtc atctttccag
    20101 ttaacggcaa acgacagcag atcgttttca cgcacatcgg ctgccagttg taatccggca
    20161 ccgtcgctgt aatcggaata atggctgtag ctgccttttt tatttttcaa atcagccagc
    20221 gagttgtaca tcatcagcgt attttcaaag gtgtcgcgat acagccgact tttcagggta
    20281 aaacggtcgt ttagttgggt cgctccctga taataaaaac tttctttgtc atactctggc
    20341 cactgccagt agcgtgattt ttgaccacaa tttccgctgt atggcgggtt atctttttca
    20401 ccatcctgct taatgtaagt cagcgtgtat tcatcgtttt cgcgcggtgt aaaaccgagc
    20461 ttcacaatgc cgcgtttatc atcagccgat gaattaatca tcttgccgtg tttgcctgca
    20521 atatcattat ttacaccatg cggcaggccg agaaaatcct gctttagctg gctaccgctg
    20581 acttgcaaat acccaagatc gctgctggcg gcaaatgaag catgcatatc gtaggcattg
    20641 tcctgactac ggctccatcc ctggcgatat cccagacttg cttcttcagg gtggtgatat
    20701 taatggctcc gcccatctga ttaggcccat gaagcagcga cgaataccct ttggaaactt
    20761 cgactgcccc cagattgttg gtcagaatcc gcgccagatc gaggttgccg tcatagggga
    20821 cataaatggg cacaccgtcg aaatagaccg gcacctgccg actatcaaag ccacgaactt
    20881 ttacctgttc ttcgttgcgg ctgcctgact tttgcagcac cacgccgggg acgacactta
    20941 atgcctgagc gacattctgt ttatccaagg ccttcatggt gggttgatcg agaacggtgg
    21001 tcgtcgtcga tgataccgga ctggaccata cggtcagcgt gtcgctctcc tgagccgcta
    21061 cggcctgctg ggtaaaggca agtgataatg ctgtgcagag ataacgtttt ttgagtctca
    21121 tagaataaat tccgtttaac aaagaattaa atagaggctg tgggttgcag actgacttta
    21181 atgtcagctg gtgccgcgta ataaggcgcg gaggtaataa aaaggcgaat gccgcagtcg
    21241 aggtaatttt tgagtgtcgt cagattaatt ccgccggtca gagccagcgt gcagtgagga
    21301 gccagcgacg gcgctatttg cgcaatttca gttgcctgtt gcggactaaa tttgtcgagc
    21361 tgaagtacgt ctggttgtgc gcgtaacgcg gcgatagcct ctttcggcgt gtcggcttca
    21421 acaacaattt tcttctcggg tgcgtggcga cgtaactgag cgattgcgcc tgaccagccc
    21481 tgattgtcat gaagaaaatg gcggtggttg gcaaacaata atatggtttc cgcacatccg
    21541 gcacgatgaa ttagtcctcc ggcagccaga attgcctgcg aagccagtaa gcgggtgccc
    21601 ggaattgctt ttcgggtgca ggcgatattg ccgtcaggat aacgttcacg aagcaatgcc
    21661 agcatttgag cgagataatc agaaacaccg caactccact ccagcacatt ctggactgcc
    21721 ttccatccct gatgaagtgc tgcggcatta ccctgcgcac ggattagccg ttgacccgcg
    21781 ttcgcttgtg aaccgtcgct gaccgcgtca tcaatagtta atcccagcgt agttaacatc
    21841 ttacacgcga cagaaacgcc gctgacgcag ccgccctgac ggtgaaaaaa cgctatatag
    21901 ccaggctggt gtccaatatt taatgctcgc gtggtcaggt cacctccctg gatatcttcc
    21961 agcagtaacg catcgatttg tgcctgagaa aggaaaatca tcacagtgcc tcctgcggta
    22021 cgctatccca ccagatgagc gcccagtcgc ggctggcagg ggcgagggca cgagcatctt
    22081 gttgttgata ccagtgatat aaccgctcac gctcatcgtc gttaagcgcg ccaagactcc
    22141 agctgacgtt ctgctcaaaa cgttcccagg tgctgttatc ctgttggcaa ttttgtccac
    22201 ggataaaatc aacgtgggca taaattccca tctgatacag gacgttgagc gcaaagatat
    22261 agttgggtaa ttcgatcact tcccgaccag ccgcccgctg aatggctgga gagacgaacg
    22321 aggtgctgac cagatgagtt gtatagacgc gaagccgtgc ctgattattc agtttgctca
    22381 ttgcatggcg catatcagcc accagagtgg agcgggaagc cacggcaata tcgcagcgtg
    22441 gcaggtcact ccagtcctct tcccaggcgc gctggatcca gtggacattg tcagccttca
    22501 gtgcggcggc gcggcgggcg gcaacgttca gcatcccctg actgtaatca acgccataaa
    22561 ccgtagtgag tttgtcagcc agcgccagcg atacggtgcc tggtccgcag cccatatcga
    22621 ataaggtctg agcgccttgc aggtcaattt tagcgatcag ttgttgcagg tagctgtccg
    22681 ttggcgaggc gcaattttcc gccattttct ccgcccgttg atcccagtga tcgggcgttt
    22741 tttctgtgcg gtgagccagt ttgagttgct gcagataaag gtctgcaaaa tcaatatcat
    22801 caatgagcat gattgttcct tagcggctaa ttgcagagag gtgatgatga atttggtcgg
    22861 cagaaacccg atacagggcc gccagtttgt tcgttgttaa ctgttcggtc ggtaagcctt
    22921 gtgttactcg tccatctggc tctacttgga taatgctgtc ggcaatggcg ttggcatgga
    22981 gcgtatggtg agtcgacatt aacattgtca tgccgttatt tttgagctgc gccagcgtgt
    23041 ccagaagttg gatctgatga ccgaaatcga ggctggaggc gggctcatcc agtaacaaca
    23101 agcgcggttg ttggacgagt gcacgggcga ttaaagccag ctgacgttca ccgccgctaa
    23161 gcgtattcca gcgtcgtgtc gccagatgca aaatgcccag cttttccagt tgctccgtgg
    23221 ctttcaggcg ttcttgctta ccgggaacgg aaaaagcgcc tatggtgggg gccaggccca
    23281 tcaacaccat atccagcaca ctgaaagcaa aaataccgtc gtgcgcctgc ggcacccagg
    23341 caatagcctg tgcgcgctgg cgatggctga gtgtctgcac agagaggcta tccagtagga
    23401 tctcgccttt taataacggt ataacgccaa gaatggagcg catgagtgtg gttttgccgc
    23461 aaccattcgc gccaagcaga caacaaattg ttccttgcga cagggtgaag gacacgtcgc
    23521 gtagcactgg atggcgggag taacccagag acgcatgacg aacggcaagc aaagtcactg
    23581 gcgacctccg cgtaacaaca aaagcaggaa aaacggtgcg ccaacaaaag cggtcagaat
    23641 gccaagtggg atttctgttg ttccaatgct gcgcgccagc gtatcggtaa gcaggagcag
    23701 aatagcgcca gtacacattg ccataggcaa cagctgctga tggttatgac cggtcaacaa
    23761 gcgggcaatg tgcggaacga ccagaccgat ccaaccaatt atgcccgcta ttgctaccgt
    23821 gctggcggtg attagtgtgg cgcagacaat caatccaaag cgcagacgcg tcacgttcag
    23881 tcccagcgaa cgggcttcgt cgtcggaaag cgtgagtaaa ttcatgcgcc agcggagaaa
    23941 aaacaggggc aaggagccta tgagaatgat cggtgcagca tagcagagat cgcgtaaggt
    24001 cacggtggac agtcccccca gcaaccagaa ggtaattgac ggtaactggg tgtaaggatc
    24061 ggccagcgtt ttaattagcg atatccccgc tccgcacaac gttcctaacg caatgcctac
    24121 cagcaccagg gttaagatcg gatcgtggcg ggtcacccga cgcgtaatca accagacgcc
    24181 cgcgaccacc atcagcccgc cgcaaaatgc gtacagctgg atgtaaacaa tgggtaatcc
    24241 ccacaggatt gccgcgcagg caccaagtcc ggcaccggcg gagacgccta aaatatccgg
    24301 tgaaactaag ggattacgca acattccctg ataggtggtt ccggctcctg ccagcgctgc
    24361 gcccagtaac agcgcagcaa gaatacgcgg cacgcggatt tgccagaaga caatttgttc
    24421 ctgcacagac tcattttctt gacgaagaat tagcgccagc agttgctgca tatcgagatg
    24481 ataagcccca cttacggcgg caaaagtgat actgatagct gccagcagca aaatacatcc
    24541 gtttacgatt ctcattagct cgccaccaat ttttgatagt cggcatcact gagtgaacaa
    24601 tgccagaaca gttgggcgta atgctgcatg tcacttttaa actggcgatt gatggcggga
    24661 tccagccagg cgtgaagtct gcgtaatccc agaagacggt tgattcccgg cggggcatcg
    24721 agccagccaa agggcaagcc acttaaaaat aagatgcgtt gttccgcaac ggctttcacg
    24781 ccctgccaga gaggatcacg acgaataaaa tctgcagtaa cggcctcctg aaccagaata
    24841 atatccggct gccagcgcag gagattttcc atggaaacct gtgtcaggcc gtggcgatcg
    24901 gctatttgcg cgacgttgtg caaacctaat aattccgccg cctcggtatg caacgaaccc
    24961 tgtaagcccg tttccagccc tcggggaccg cgcgcagcat aaaagcggag gttagcggcg
    25021 ggtgaggtgg cgaatgcctg agcttcacca acgaagtggc tggcgagatt ggcttgttct
    25081 gcggcgcggt gctcttcgcc taacgttttg ccaagcgttg ttaactgttc tgctgattgc
    25141 gccagttttc cgttaagcaa taaccagggg atttgtgtct gttcgctaac ctgccgtgcc
    25201 tgggagatca aggtttcatc cgtattgccg caatcaacga ccaaatcggg atgtaacgcc
    25261 ataagccctt ccagcgatag tgtgctggcg cgcccggcga gtcgtcccag cctgggtaac
    25321 tggcgaatgt gctctggcaa tggaattaat gcctgacggg caaaatcaaa agaggaaaaa
    25381 ccgaccattt tttctggcgc gacagccagc aagagcaaat cggcgggcgc gcctgcgctg
    25441 acgatgcgtg taatgttttg ggcttcggga agagattgcg aagcaaaacg attaactgtt
    25501 tgtgaaaagg acggtagaga ctgcaggagc agggtagatg caagtgcctg cgcaaacgtc
    25561 cgtctggtta tgggcatggt gaacatcctg aaaagtaaaa tcgatatata ctttcatata
    25621 tatcgataaa aatcatgttc aatatcaaag tttagctatt gaaaacatga agatgtgacg
    25681 acttccgcaa gcctgttgac cctctctatc attaaaaatg tatttatcag gtggctaata
    25741 ttatttaaat catttaaggc taaattattt aaatatgaat gacattattg tttttttatc
    25801 gtccacggtt acgcgacgtt gaagttactt gttttataat gagcgatttt tgatatctcg
    25861 ccgagaaaat tatcagcatt ctctcgtttc agtgtcgtga atatactgtt aacggaggtg
    25921 aaaagaatgc tgggggttat acaaaaccta tgttgttgtc gtccggattc accgcttgct
    25981 gatagtaagc agcgtaacca gaagttaccg caacaagact ttgaggattt taagaacgac
    26041 gttattaatg tcattaacaa caatgaagat atttattttc ttagagagag taatttactg
    26101 aatgcgttta ttcggacgtg caaaaaaaga gatgataaca gttacggaaa tatgatattt
    26161 acgctttatt ttgataacga tatctatggg aacagaatta tcaaagcatc gctgaacggc
    26221 ggagagttgc agttattaag cacagggtgt agcaatgata acattctcca t
//
LOCUS       DAJTRN010000055        25834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRN010000055 DAJTRN010000000
VERSION     DAJTRN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO328_002335"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO328_002335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5930881.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO328_002336"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO328_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5930882.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO328_002337"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO328_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5930883.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO328_002338"
     CDS             3807..3995
                     /locus_tag="OO328_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5930884.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO328_002339"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO328_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5930885.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO328_002340"
     CDS             4756..5115
                     /locus_tag="OO328_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5930886.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO328_002341"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO328_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO328_002342"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO328_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5930887.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO328_002343"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO328_002343"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5930888.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO328_002344"
     CDS             10913..11254
                     /locus_tag="OO328_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5930889.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO328_002345"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO328_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO328_002346"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO328_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5930890.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13680..14966
                     /gene="dctA"
                     /locus_tag="OO328_002347"
     CDS             13680..14966
                     /gene="dctA"
                     /locus_tag="OO328_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5930891.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15187..16683
                     /locus_tag="OO328_002348"
     CDS             15187..16683
                     /locus_tag="OO328_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5930892.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16779..17708)
                     /gene="kdgK"
                     /locus_tag="OO328_002349"
     CDS             complement(16779..17708)
                     /gene="kdgK"
                     /locus_tag="OO328_002349"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5930893.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17940..18707
                     /gene="pdeH"
                     /locus_tag="OO328_002350"
     CDS             17940..18707
                     /gene="pdeH"
                     /locus_tag="OO328_002350"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5930894.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18777..20837
                     /locus_tag="OO328_002351"
     CDS             18777..20837
                     /locus_tag="OO328_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5930895.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21019..22341)
                     /locus_tag="OO328_002352"
     CDS             complement(21019..22341)
                     /locus_tag="OO328_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5930896.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22752..23765)
                     /gene="yhjD"
                     /locus_tag="OO328_002353"
     CDS             complement(22752..23765)
                     /gene="yhjD"
                     /locus_tag="OO328_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5930897.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23814..24713)
                     /locus_tag="OO328_002354"
     CDS             complement(23814..24713)
                     /locus_tag="OO328_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5930898.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25233..>25834
                     /locus_tag="OO328_002355"
     CDS             25233..>25834
                     /locus_tag="OO328_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5930899.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6156 a         6491 c         7030 g         6157 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc ccccccccca taaccttaca agacctgtgg ttttactaaa ggacacccta
    13681 tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta
    13741 ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct
    13801 tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca
    13861 ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact
    13921 ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc
    13981 ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg
    14041 ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg
    14101 tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg
    14161 gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt
    14221 tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgc
    14281 tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc
    14341 agctgattat ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa
    14401 tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc
    14461 tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg
    14521 agaaactcgg ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta
    14581 accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta
    14641 acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta
    14701 aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg
    14761 tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag
    14821 aagctcgtgc gctaactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt
    14881 gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg
    14941 gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag
    15001 tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg
    15061 tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta
    15121 acgtcattta gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg
    15181 gttcacatgc agggcataaa aattcgactt ttagcgggcg gtttgctgat gatggccact
    15241 gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt
    15301 tccaacggtt tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa
    15361 attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt
    15421 cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt
    15481 tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat
    15541 gacaccacgc tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg
    15601 ctctcttatc tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac
    15661 gcgctgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg
    15721 cgctatcgtc tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa
    15781 cccgttgaag cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg
    15841 acgctactgg tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg
    15901 tttggcgaac tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg
    15961 cgtgcggaag cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg
    16021 tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg
    16081 gacctggccc gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtgacagc
    16141 aaagacatcg gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc
    16201 aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg
    16261 gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag
    16321 aaactggagc tgcagaaact gtttgccgcc tatgcacggg ctgataccga tattctgatg
    16381 ggccagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag
    16441 aaactgcggc aggatttcct taacagcctg actgtggaga tgttaaatca ggatctgcgt
    16501 cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt
    16561 aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatctac cgccgctgcg
    16621 accacctctg tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag
    16681 taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga
    16741 ggggtaacac tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc
    16801 gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc
    16861 gcgttttccg cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg
    16921 ccagctgcgg tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatcaact
    16981 aacgcttcac cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct
    17041 ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc
    17101 gcgtcttcgt cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgctggtac
    17161 acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc
    17221 acttttccgc ctttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc
    17281 gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct
    17341 tcgcaaatcg ccgcagactg ctcgctcgcc agccagaatt tggcggcggc ttcgttacgc
    17401 cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggaaga
    17461 cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc
    17521 atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca
    17581 ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc
    17641 ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt
    17701 ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt
    17761 tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg
    17821 aaagattccg ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgccctta
    17881 cgcgccgata atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga
    17941 tgataaggca ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg
    18001 aacggcgttt ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat
    18061 gcgggcggtt aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc
    18121 aacgcctgcc gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg
    18181 tgaaagagca gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacggcctgc
    18241 tggcatcggt caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc
    18301 tgcgccagat tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc
    18361 cgaaagattc aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg
    18421 gtaccgggat ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg
    18481 cgcgagaact gtttgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc
    18541 ttttacatct aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg
    18601 aagagtggcg tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac
    18661 gcccggcacc gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt
    18721 ccgccgggct ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga
    18781 gcaaggcagg caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg
    18841 cgatcatttt gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag
    18901 tctctgcgga gttgaatcgt ccgttcgcta tccgtggcaa tctgggcgtg gtgtgggagc
    18961 ggcaaaaaca agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag
    19021 acatcattct tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag
    19081 tagaggcaac gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca
    19141 agctcgaaaa gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt
    19201 ttaatcttgc caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc
    19261 ggctggataa tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag
    19321 cggatctgga aatttttgtt gatccgttag gcaagccgct gccattcagc gaagttactg
    19381 gatcgaaagg taaagcggat aaagaaaagg tgggcgatta catttttggc ctgaaggcgc
    19441 agggacgtta taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc
    19501 tgcgtggcga agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg
    19561 ttgcttttga tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta
    19621 aattttctgg cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata
    19681 ccccgccgtt tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg
    19741 tctttgatta tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg
    19801 tctacaccac cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc
    19861 ggctggcgga cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga
    19921 aacggtctga acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt
    19981 atgaccgctt cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc
    20041 ggcgcattga gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca
    20101 aaaatgctga cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg
    20161 cgaatattca tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc
    20221 gtcgactgaa actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg
    20281 aaatgaacgg tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca
    20341 acagtaacgg caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg
    20401 agattgttgg gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg
    20461 tgcgggtgaa ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga
    20521 tttttgcttt cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt
    20581 cggaacagct ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc
    20641 gttcgccgct gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac
    20701 cgctgattgc ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg
    20761 cgttactggc actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt
    20821 cgcagatgaa gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt
    20881 agatttttgg caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    20941 tacaaatcct tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca
    21001 tcaggctttt cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc
    21061 gattaacgtc aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt
    21121 agcctgcaac caggctgcga tatacggcgc aacggaagcc ccgagaatcg acgatacgtt
    21181 gtaagagaac gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc
    21241 acccatcggc ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac
    21301 cagaatcggg ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat
    21361 cagcgtggtg atgatcacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc
    21421 gactggcacc atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt
    21481 acgcggcagg ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt
    21541 catgatgtaa aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg
    21601 aacatgtttg gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac
    21661 tttctcaaac accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag
    21721 caccgccgag aagataaatg gcacacgcca gccccagctc ataaactgct catcggtcag
    21781 tagccaggaa agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag
    21841 ctgtgggaag gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag
    21901 cgccgcgccg ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag
    21961 cagcagtggg gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt
    22021 cgaaatcccc atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc
    22081 aaaatgacca aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc
    22141 gagcgactgt agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac
    22201 aataacggcc gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag
    22261 agaggcgaca aggactttat tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag
    22321 tgttgtggct gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta
    22381 cgcacagcaa aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc
    22441 aaaaagggga taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt
    22501 ataaatagtg gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc
    22561 tggggttatg agttggagtg aaattgtttg ctcccaaata gatggctgaa gaggctctgg
    22621 ggaagagttt caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat
    22681 ccggcctacg gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca
    22741 tccggcctat tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg
    22801 gtggcaatcc acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc
    22861 agccccagca cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg
    22921 taggtcatca cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc
    22981 agggcttttt tgcgcggacg gtggcgcggc aaccgccaga agatccagaa gaaaagcaga
    23041 tagttggcga agatggaaat cgccaggcca atcaatcgcc acgtcggttt cagccactca
    23101 atgctgttca ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg
    23161 atcgaaagcg tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa atcacgcaga
    23221 tattttaccc agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg
    23281 gcacgaatcg cttcacgcag gttacccatc cagttgatgc cggaataaag tgccaccgcc
    23341 aggccgacaa gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac
    23401 gtggcggcta gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc
    23461 aacatcggat gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg
    23521 atcatcgaca aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg
    23581 ttaaagcgtt ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg gactttttcg
    23641 gcagtggtgg tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc
    23701 aactgcttaa tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc
    23761 gtcatccgct cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt cggtcagtcc
    23821 aggtgctctt tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc
    23881 tcccgacgct gcgggtaaac cagcgaaagg gagagcggct cggcacggta gcctggcaac
    23941 acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata
    24001 atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg
    24061 ccgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt
    24121 acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc ggggctttgc
    24181 ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt tagtttgccg
    24241 aggggacggg cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa
    24301 ccatcatgaa gaatatctac cggacggtta tggctactca gttccagctc aattcccgga
    24361 tgctgataga gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg
    24421 atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt
    24481 tcgctgagat tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg
    24541 agtttgaccc gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc
    24601 tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa
    24661 ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg
    24721 accgattgtt gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct
    24781 aacatctaat gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga
    24841 aaagggcgga atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt
    24901 gattcccttt aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg
    24961 ccatttgtta ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc
    25021 accaatataa ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg
    25081 cgtttagctg taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg
    25141 ccaccgccaa atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag
    25201 tctttttaac gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt
    25261 caatatttat tcatggaata aaaatcagtt tacagcagcg tattccagga gtgagtattc
    25321 agggggtcag tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag
    25381 ttatgctcga tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg
    25441 tgcagtttcc tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac
    25501 aggaagttat ccattttaat gtgctggcca ttgtgcctcg cgattcaacc gtcgagacat
    25561 ttgcgttggc ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga
    25621 cgacgccgga aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt
    25681 taaccatgtt agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta
    25741 ccggaacggt aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca
    25801 cccaggcggc aatgatgtta aatatctcct cctg
//
LOCUS       DAJTRN010000056        25743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRN010000056 DAJTRN010000000
VERSION     DAJTRN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..132)
                     /locus_tag="OO328_002356"
     CDS             complement(<1..132)
                     /locus_tag="OO328_002356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5930900.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     "
     gene            complement(265..570)
                     /locus_tag="OO328_002357"
                     /pseudo
     CDS             complement(265..570)
                     /locus_tag="OO328_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            638..1253
                     /gene="tnpA"
                     /locus_tag="OO328_002358"
                     /pseudo
     CDS             638..1253
                     /gene="tnpA"
                     /locus_tag="OO328_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1581..2663
                     /locus_tag="OO328_002359"
     CDS             1581..2663
                     /locus_tag="OO328_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5930901.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3152..3950)
                     /locus_tag="OO328_002360"
                     /pseudo
     CDS             complement(3152..3950)
                     /locus_tag="OO328_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3960..4511)
                     /gene="ybcL"
                     /locus_tag="OO328_002361"
     CDS             complement(3960..4511)
                     /gene="ybcL"
                     /locus_tag="OO328_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5930902.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4680..5012)
                     /gene="emrE"
                     /locus_tag="OO328_002362"
     CDS             complement(4680..5012)
                     /gene="emrE"
                     /locus_tag="OO328_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5930903.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5357..5671)
                     /gene="fliE"
                     /locus_tag="OO328_002363"
     CDS             complement(5357..5671)
                     /gene="fliE"
                     /locus_tag="OO328_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5930904.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5885..7544
                     /gene="fliF"
                     /locus_tag="OO328_002364"
                     /pseudo
     CDS             5885..7544
                     /gene="fliF"
                     /locus_tag="OO328_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7537..8532
                     /gene="fliG"
                     /locus_tag="OO328_002365"
     CDS             7537..8532
                     /gene="fliG"
                     /locus_tag="OO328_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5930905.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8525..9211
                     /gene="fliH"
                     /locus_tag="OO328_002366"
     CDS             8525..9211
                     /gene="fliH"
                     /locus_tag="OO328_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5930906.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9211..10584
                     /gene="fliI"
                     /locus_tag="OO328_002367"
     CDS             9211..10584
                     /gene="fliI"
                     /locus_tag="OO328_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5930907.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10606..10947
                     /gene="fliJ"
                     /locus_tag="OO328_002368"
                     /pseudo
     CDS             10606..10947
                     /gene="fliJ"
                     /locus_tag="OO328_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10944..12071
                     /gene="fliK"
                     /locus_tag="OO328_002369"
     CDS             10944..12071
                     /gene="fliK"
                     /locus_tag="OO328_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5930908.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12176..12640
                     /gene="fliL"
                     /locus_tag="OO328_002370"
     CDS             12176..12640
                     /gene="fliL"
                     /locus_tag="OO328_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5930909.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12645..13649
                     /gene="fliM"
                     /locus_tag="OO328_002371"
     CDS             12645..13649
                     /gene="fliM"
                     /locus_tag="OO328_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5930910.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13646..14059
                     /gene="fliN"
                     /locus_tag="OO328_002372"
     CDS             13646..14059
                     /gene="fliN"
                     /locus_tag="OO328_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5930911.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14062..14427
                     /gene="fliO"
                     /locus_tag="OO328_002373"
     CDS             14062..14427
                     /gene="fliO"
                     /locus_tag="OO328_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5930912.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14427..15164
                     /gene="fliP"
                     /locus_tag="OO328_002374"
     CDS             14427..15164
                     /gene="fliP"
                     /locus_tag="OO328_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5930913.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15174..15443
                     /gene="fliQ"
                     /locus_tag="OO328_002375"
     CDS             15174..15443
                     /gene="fliQ"
                     /locus_tag="OO328_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5930914.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15452..16237
                     /gene="fliR"
                     /locus_tag="OO328_002376"
     CDS             15452..16237
                     /gene="fliR"
                     /locus_tag="OO328_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5930915.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16522..17145
                     /gene="rcsA"
                     /locus_tag="OO328_002377"
     CDS             16522..17145
                     /gene="rcsA"
                     /locus_tag="OO328_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5930916.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17189..17377)
                     /gene="dsrB"
                     /locus_tag="OO328_002378"
     CDS             complement(17189..17377)
                     /gene="dsrB"
                     /locus_tag="OO328_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5930917.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17540..17767
                     /gene="yodD"
                     /locus_tag="OO328_002379"
     CDS             17540..17767
                     /gene="yodD"
                     /locus_tag="OO328_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5930918.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18065..18880
                     /locus_tag="OO328_002380"
                     /pseudo
     CDS             18065..18880
                     /locus_tag="OO328_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18877..20571)
                     /gene="yedQ"
                     /locus_tag="OO328_002381"
                     /pseudo
     CDS             complement(18877..20571)
                     /gene="yedQ"
                     /locus_tag="OO328_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20742..20924)
                     /locus_tag="OO328_002382"
     CDS             complement(20742..20924)
                     /locus_tag="OO328_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5930919.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21003..21920)
                     /locus_tag="OO328_002383"
     CDS             complement(21003..21920)
                     /locus_tag="OO328_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5930920.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22093..23013
                     /gene="yedA"
                     /locus_tag="OO328_002384"
     CDS             22093..23013
                     /gene="yedA"
                     /locus_tag="OO328_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5930921.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23002..23472)
                     /gene="vsr"
                     /locus_tag="OO328_002385"
     CDS             complement(23002..23472)
                     /gene="vsr"
                     /locus_tag="OO328_002385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5930922.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23453..24871)
                     /gene="dcm"
                     /locus_tag="OO328_002386"
     CDS             complement(23453..24871)
                     /gene="dcm"
                     /locus_tag="OO328_002386"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5930923.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24938..25478)
                     /locus_tag="OO328_002387"
                     /pseudo
     CDS             complement(24938..25478)
                     /locus_tag="OO328_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25518..>25743)
                     /locus_tag="OO328_002388"
     CDS             complement(25518..>25743)
                     /locus_tag="OO328_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930924.1"
                     /translation="TWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALV
                     GFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6491 a         6599 c         6343 g         6310 t
ORIGIN      
        1 ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
       61 tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt
      121 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt
      181 tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc
      241 atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt
      301 gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat
      361 ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg
      421 gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac
      481 gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa
      541 ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa
      601 aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt
      661 taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
      721 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      781 cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca
      841 gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt
      901 attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc
      961 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac
     1021 gagccttata tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga
     1081 ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc
     1141 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
     1201 acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc
     1261 tcaattacat taataaatct aaataaatat catgcaaata atttccatgg ttatttttag
     1321 gctatttaaa ttaaaaaaat aaattatgag tcaatcataa ataacaacct ttaatataat
     1381 ttgactacca aatttatatt cgttcacaaa atagtcatgc attgtgtgca atagaaacag
     1441 ttcagataaa aatagggatc tacttcacaa tttaaatgag aaactaaaac ttacatcttg
     1501 aaataatctc attgattgga tgaatatttg tagtgcagtg acatcatttt taaataatag
     1561 ttcaaataaa agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat
     1621 cagtactgat ggcgatgtct gctcaggcag cagagattta caataaagac agtaacaagc
     1681 tggatctgta cggaaaagtt aatgctaagc actacttctc ttctaacgat gcagatgatg
     1741 gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac tcaaatcaac gatcaactga
     1801 ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg tgctgaatct caaggttctt
     1861 ccaaagataa aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg
     1921 attatggccg taactacggt gtagcatacg atatcggtgc atggaccgac gttctgccag
     1981 aattcggtgg cgatacctgg acccaaacag atgtgttcat gactggtcgc accacaggtg
     2041 ttgcaaccta tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc
     2101 agtatcaggg taaaaatgac cgcactgacg taactgaagc taacggtgat ggtttcggtt
     2161 tctccactac ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc
     2221 gcactaatga tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg
     2281 aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt
     2341 ctgaaaccca gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact
     2401 tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc
     2461 aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga cctgattgaa tatattgatg
     2521 taggtgcaac ctattacttc aacaaaaata tgtccacttt tgttgattac aaaatcaacc
     2581 tgattgataa gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg
     2641 taggattggt ttaccagttc taatctgatt actcaaagat atgttgtggg aggctttgcc
     2701 tccccaacat ataagtggcg ctctcaagcc actttcctta gaagcactat cttgcttctg
     2761 actatataaa ttacccttta ttttctggga cgtttcaacg ccccattttt aataatactc
     2821 agtgaacaat taacgtatca attagattta ataacaacga tatccgtacc taacctgata
     2881 tctaatgtca taagcatccc tttaattatc ccctcaacct cttgtaaatt tcccactctt
     2941 gagctccaca tagcccaaac gccggatgca aaaaaaatca tacgtgcatc tctgaaattg
     3001 tgatggagga agtgagtatc aattatatgt gcttataaac gttcccgatt ggcacagaag
     3061 tcaccggatt gttcaggctc caataggttg agtatgtcgt atttattttc caaaataaaa
     3121 atcgatgctg ttgctcctta ataaaatact tttactctct ggacgctttt cttagctctc
     3181 ttggtgttat attaaaacga ttatggaacc tttcagtaaa acgagaagga cacttgtaac
     3241 cattttccct ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca ggacaacgca
     3301 ttatttaacc tcacgtcgtc caatatactt tgaaaactta ttccctcgct tgccagacgg
     3361 cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc tgtccatttt
     3421 gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg tttactatct
     3481 gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc cattgctaag
     3541 tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg ctcccaacaa
     3601 tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat ttgatcagtt
     3661 atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag catttctgca
     3721 agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca gggaaataat
     3781 attatctgat tattatcaac agttagacta tgcgtctccc aattgataac tttttttccc
     3841 tgacggatac gacataaagc tggcataaga ggcttaaccc tgtgaatctc atgatgttta
     3901 tgcatccgta tttcttcaat ctttaggtta gacttatctc ttgccagcat actcttaccc
     3961 tattttatct cataaactgg tgttatctca gcggttgcaa ttttattggc attaagcata
     4021 taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt ttttagagcc
     4081 catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc tccaccaaat
     4141 cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt tgtaccatca
     4201 cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc aacagtccag
     4261 tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc aaagcttttg
     4321 gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc ttcacaccca
     4381 aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc attactagtg
     4441 acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact tgaaacgata
     4501 agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga tgtgccttga
     4561 tctcattatg cataaaggcg caatgatcgc gcaaatgcaa tcataattat aaaggtgccc
     4621 cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag aattattttt
     4681 taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca aatcaacatc
     4741 atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga aagcaaacta
     4801 atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg tatataagcc
     4861 agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc aacagagggc
     4921 cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac ctctgcaagt
     4981 attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt catactggag
     5041 agaattgtac tacagtttga acacaactca ccgttttcat cctggtgtat gtgtatccaa
     5101 ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag gaaccccagg
     5161 ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt tatcgcgttt
     5221 acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac cagaaattga
     5281 gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa caagcatagt
     5341 caacaggtta cacaacctac acctgcatgg acatcacttc ctgatacgcc gccaccagct
     5401 tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca
     5461 catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc gtgcgggcga
     5521 ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg
     5581 tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt aactggctga
     5641 taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt tctggtaaca
     5701 aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa actggtttat
     5761 tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtgtgctga
     5821 cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag ccacgaagtg
     5881 cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc gcctgcgtgc
     5941 gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca tggtcgcact
     6001 gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt ccgatcagga
     6061 tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct tcagcgaagc
     6121 cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgg cgcaacaagg
     6181 tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat caggaaaagt ttggtatcag
     6241 ccagttcagc gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat
     6301 cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt
     6361 attcgtccgt gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg
     6421 cgcactcgat gaagggcaaa ttagcgctat tgtacatctg gtttccagcg cggttgctgg
     6481 tctgccgcct ggaaacgtca cgctggtgga tcagggcggg catctgttaa cccagtccaa
     6541 taccagcggg cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg
     6601 tattcagcag cgtattgaag cgatcctgtc acctattgtt ggtaacggta atattcacgc
     6661 ctaggtcacg gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc
     6721 taacggtgat gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag
     6781 cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa
     6841 taacgcgcca atcagcacgc ctccgacaaa tcaaaataac cgccagcagc aggcgagcac
     6901 caccagcaat agtgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga
     6961 tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt
     7021 agtggtgaat tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat
     7081 gaagcaaatt gaagctctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc
     7141 gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa agcggcggag cactgccgtt
     7201 ttggcaacag caagtgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc
     7261 tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc
     7321 gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc ccgcgaggaa gtggaagatg
     7381 cggtggaagt ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac
     7441 tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg
     7501 tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat
     7561 aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac
     7621 ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc
     7681 aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgctgca
     7741 ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa
     7801 cgtgccgcca gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa
     7861 acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa
     7921 attatcgcca ccattctggt gcatctgagg cgcgcccagg ccgccgatat tctggcgttg
     7981 ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag
     8041 ccagccgcgc tggccgaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc
     8101 aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg
     8161 cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa
     8221 atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag
     8281 cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag
     8341 ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac
     8401 gatctcgcca agcgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt
     8461 ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat
     8521 acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc
     8581 ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag
     8641 ccttgagcag caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcggg
     8701 gattgccgaa ggtcgccagc aaggtcacga acagggctat caggaaggac tggcccaggg
     8761 gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca
     8821 gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg
     8881 cctgatgcag atggcgctgg aggcagcacg tcaggtgatc ggtcagacac caacggtgga
     8941 taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg
     9001 gaaaccacag ctgcgcgtac acccggatga tctgcaacgt gtggatgata tgctcggcgc
     9061 aactttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccacc ctggcggatg
     9121 taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact
     9181 ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc
     9241 acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga
     9301 ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca
     9361 acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt
     9421 ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc
     9481 ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg
     9541 ccgctcggtc cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc
     9601 ctgcccttcc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg
     9661 caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt
     9721 accgtcggac gcgggcagcg tatggggctg tttgccgggt cgggcgttgg taaaagtgtg
     9781 ctgctgggga tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg tttgattggt
     9841 gaacgtgggc gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca
     9901 cgctcagtgg tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc
     9961 gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt
    10021 atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa
    10081 ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag
    10141 cgtgccggta atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc
    10201 gaaggcgatg accagcagga ccccattgct gactccgcgc gggcaatcct cgacggtcac
    10261 attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg
    10321 tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc
    10381 ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat
    10441 gccaaaggca gcgatccgat gctcgataaa gccatcgccc tgtggccgca gctggagggc
    10501 tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag
    10561 cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcgga agaacagctc
    10621 aaaatgttga tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc
    10681 gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc
    10741 attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac
    10801 agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc
    10861 acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag
    10921 cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg
    10981 acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt
    11041 tgagcgaagc attagcaggc gagacaacaa ctgacaaagc ggccccccag ttgctggtag
    11101 caacagataa gcccacgaca aaaggcgagc cgctggtcag cgatattctt gccgatgcgc
    11161 aacaagcgga tttactgatc cctgtggatg aaacacttcc tgtcatcaac gacgaacaat
    11221 ccacatcaac accgttaacc accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa
    11281 atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg
    11341 ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa
    11401 ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacta
    11461 cagcacagcc tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag
    11521 aagcccagag taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc
    11581 cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg
    11641 caccgctggg ttctcacgaa tggcaacaat cattgagcca gcatatttcg ctgttcaccc
    11701 gccaggggca acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa
    11761 tttccctcaa agtggatgat aaccaggcgc agatccagat gatttcaccg catcagcacg
    11821 tacgcgccgc cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca
    11881 ttcagttagg gcaaagcaac attagtggcg aaagctttag tggtcagcag caggccgctt
    11941 cccagcaaca gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg
    12001 atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc ggcgttgata
    12061 ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct
    12121 caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac
    12181 tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat
    12241 taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc
    12301 tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt
    12361 tacggtcaat ttgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgactgaa
    12421 agatgaagca acccgctcgc ggctgagtga gtatttgccg gaagtccgta gtcgcttgct
    12481 gttactgttt tcacgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct
    12541 gattgccgag attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga
    12601 tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc
    12661 tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga
    12721 cagccagtat tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg
    12781 ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc
    12841 gtatggggct gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca
    12901 ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc
    12961 acctgaaacc gctacgcggc acagggctgg tagtgttctc gccgagtctg gtgtttatcg
    13021 ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt
    13081 ttacccatac tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg gaaggctata
    13141 gcgacgcctg gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg
    13201 tggaatttac caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg
    13261 tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc
    13321 cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact
    13381 ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggaactggtg gccaactttg
    13441 ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga
    13501 tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt cccggttctg accagtcagt
    13561 acggtactct caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa
    13621 attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa
    13681 cggcgcaatg gacgatctgt gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa
    13741 aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca
    13801 ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg
    13861 gatgaccatc aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct
    13921 ggcgggcgaa ccgctggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt
    13981 ggttgttgcc gataaatatg gcgtgcggat caccgatatc attaccccgt ccgagcggat
    14041 gcgccgcctg agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt
    14101 tctgctgagc cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc
    14161 gctgctgcct ggctggtaaa acggctggga tttgccccta aacgcactgg cgttaacggt
    14221 ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg
    14281 gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt
    14341 cccccttcag caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaat
    14401 ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc
    14461 tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctac
    14521 ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga
    14581 cgttcattcc agcgatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg
    14641 gtttattgcg taacgcgctg ggaacgccct ccgcgccacc taaccaggta ttgctggggc
    14701 tggcactgtt tttgaccttt tttattatgt cgccagtgat cgacaaaatt tatgtcgatg
    14761 cgtaccagcc attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggcgc
    14821 agccgctgcg tgagtttatg ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca
    14881 gactggcgaa taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc
    14941 cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc
    15001 cttttttgat tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg
    15061 ttccccccgc caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct
    15121 ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac
    15181 ctgaatcggt catgatgatg ggaactgaag cgatgaaagt cgcgctggca ctggcagccc
    15241 cgctgttgct ggtagcgctg gtcacgggcc ttatcatcag tattttgcag gcagccacgc
    15301 agattaacga aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatca
    15361 ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta
    15421 acctgccgta tatcatcggg tagcccgtac aatgatgcag gagacaagcg atcaatggct
    15481 ttcctggtta agcctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc
    15541 gccaattctg agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat
    15601 cacgttcgcc attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc
    15661 tctgtggctg gccgtacagc agatcctgat cggcattgcg cttggtttta ccatgcaatt
    15721 tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt
    15781 tgcgacattt gtcgatcctg gcagccatct caatatgccc gttttagcgc gcatcatgga
    15841 tatgctggca ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct
    15901 ggtcgatacc tttcacaccc tgccgattgg tagcgaaccg ttgaacagca atgcgtttct
    15961 ggcacccacc aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct
    16021 cattactctg ttgctgaccc tgaatctggc attaggttta ctgaatcgta tggccccgca
    16081 attatccatt tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc
    16141 ggcattaatg ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt
    16201 gctggctgat attattagtg aattaccatt aatataattc cgtaacgttt atcatgctac
    16261 cctaaggatt atccgaaaaa taatatctgc gaacatcttc caggatactc ctgtagcgaa
    16321 atatttgttt taagctcact cacatatcgc aacatttact ttaagacaat tccaggcaaa
    16381 ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag
    16441 gtgacccatg ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta
    16501 cgcattgagt gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga
    16561 ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt
    16621 gaaaccgttg atgaccttgc catagcttgt gattcacagc gtccttcagt ggtgtttatt
    16681 aatgaggact gtttcatcca cgatgcttct aacagtcagc gcatcaagca catcattaat
    16741 caacatccca atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa
    16801 tatctattgg tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc
    16861 gacgatatcc ttggcgatat tctgaaaaaa gagacaacga taacctcgtt tttaaatatg
    16921 ccgacgttat cattgagccg aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag
    16981 ggaaccattc aaatctccga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa
    17041 ggtaatatta aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga
    17101 ctgacggata atgtgactaa tggtattttt gtcaacatgc gctaacacat tctgactggt
    17161 ggtttcccac cagtcaggct gaataagatt actctgcttt ctcaacaaag ataccgtcct
    17221 gatgccctgc ttcattaaag aaccagatgc cgagcgggta gtcttccagc gcaaccaggt
    17281 acattgtgcc ttcactaaac tcctcaactg ccagtaccac tccaggacga cgcggaccgc
    17341 catccgtttt gactgttacc cgatcattca ccttcatcgt tttcctcctg tggctttgtg
    17401 ccagtgtaga acaatttcgt ttttctggca gcgccaagcg cgcgcgagtg ctgattttct
    17461 cgacggtcta tacttaagag atgccaggcg gacttaacga ctggcggcaa caacagagta
    17521 acggttgcga ggaaagatga tgaaaaccgc aaaagtgtac agcgataccg caaaacgtga
    17581 ggtcagcgtc gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt
    17641 tcatcgcagc cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg
    17701 gcgtgaattg gcggatgcct tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa
    17761 tcgttgaatg cacaataaaa aaatcccgac cctgagggtg tcgggatgaa acttgcttaa
    17821 gcaagaagca cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct
    17881 gcaatttttt tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa
    17941 tagaaatgtg acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa
    18001 aaatcatctt tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc
    18061 gaccatgttt tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct
    18121 ggacagtcat agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc
    18181 caatattccc gtcattctct gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa
    18241 aatgttgggg ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc
    18301 tgaacaatgg caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga
    18361 aatctgccag gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga
    18421 tgatgtcgac gatgcaacca ttgccgaatg gacgggatta agccgtagct aggcggcgct
    18481 gacgcagctg catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc
    18541 acaatttatc gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg
    18601 gcacgtactg gatgcctctg ccggaaaaga tcaggctgcc aactggatta tcgcgaccta
    18661 tcaacaattg tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc
    18721 gcctttactg gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt
    18781 acatctgcat gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg
    18841 ctggcgtgaa gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac
    18901 ccgattgcgc ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg
    18961 ttcaaaatca taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg
    19021 tatcgttgta ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc
    19081 tgcgacctcc gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc
    19141 accgacccgc ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg
    19201 agaaagaaca cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc
    19261 aagatcgacc tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg
    19321 acgggctttt tcgaacagtg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc
    19381 ctgccactgc aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata
    19441 ccaggagagg agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat
    19501 actgccgaaa tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc
    19561 gaaatggtcc agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt
    19621 gtcatgttcc attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt
    19681 tggatggtca ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc
    19741 accatcgagg tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat
    19801 actcatcccc agcacgccat accagtagtt attactgtct accggaacac tgacggtaac
    19861 ctgcggttca gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg
    19921 acgattttct cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg
    19981 cgttggtaca ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga
    20041 gacatacatc gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc
    20101 aacttccagt gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggaaatt
    20161 cccctcgctg actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag
    20221 ttcgatttgc caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag
    20281 agaggtgaaa tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag
    20341 tttgtcgacg ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt
    20401 acgctagctg gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt
    20461 ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg
    20521 tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac
    20581 tcccaacctg cttttttatg attctggcat aacccggcaa tagcgtaccg caaaacagcg
    20641 ataattttat aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca
    20701 ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa
    20761 cgactcccct cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa
    20821 ccgtcatacc aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg
    20881 ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt
    20941 cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag
    21001 tattaatgtg cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg
    21061 ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca
    21121 ctttgctgcc cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca
    21181 atcccgccgc cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc
    21241 caaggcgcga taatcaataa ccctttacct aatgcctgca tcagcgcgct agatttttcc
    21301 gccagccaat accctaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc
    21361 acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcctct
    21421 gccacaatcc ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc
    21481 gcccctttaa ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga
    21541 cgctgctggc tctgcgctgg atcttcttta tgtttacgcg cctccagcat atgcagcact
    21601 ttctctaccc cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc
    21661 cacgggataa acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc
    21721 gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact
    21781 tgttgcgcat tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt
    21841 ttgcccatca cagagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa
    21901 ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc
    21961 aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa
    22021 ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg
    22081 ccgtgaggga ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg
    22141 tatatcattt ggggctcaac ctattttgtc attcggattg gcgtgggaag ctggcctccg
    22201 ttaatgatgg cgggcgttcg attccaggca gccggtattt tattgctggc atttttgcta
    22261 ctgcgcggac acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg
    22321 ttattgctgg ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc
    22381 ggcatcgccg ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg
    22441 tttggcatta aaacgcgcaa actggaatgg gtgggtattg ccattgggct tgccggaatc
    22501 atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta
    22561 atcggctcga ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta
    22621 gggatgatgg cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg
    22681 atgattgcgg gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc
    22741 tatctggcgc tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat
    22801 gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg
    22861 ggtacgggac tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt
    22921 gttttcgcgg tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc
    22981 gcgccagtta ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc
    23041 gctggcacct tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag
    23101 cttctcgcgc ctacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg
    23161 caagcgactg atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa
    23221 ctcagttcga gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc
    23281 gtgggtaaat atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact
    23341 ggcgtcctga acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc
    23401 aatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg
    23461 aacgtcgacc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct
    23521 caagcagttt tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg aactggcgat
    23581 aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca
    23641 ttaagcgcgc gcattcacga ggcgttaacc gacgtggacg atgttgctga ttcagcggat
    23701 cgtcaaagtc tttctcaccc gtggccatat cccaaccgcg atcgattaaa atttccgcgc
    23761 catctttgta ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa
    23821 ccattccata accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt
    23881 acttccacag caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg
    23941 ccagcgtcac tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt
    24001 tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga
    24061 aatgtttacc atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt
    24121 catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac
    24181 tcttcaggtt tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc
    24241 gcaccacatc aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga
    24301 gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta
    24361 aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc
    24421 cttcttgatg actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt
    24481 cgcaataatg gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca
    24541 cgcactgtcc accaatcgat tcaaaaccac gacggatgcc gccaattccg gcaaatagat
    24601 cgataaagcg aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca
    24661 gatgggcaaa ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt
    24721 ttaaaattgc cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcattt
    24781 taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt
    24841 atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg agatggccga
    24901 caggttaaca taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat
    24961 ctatcacctt gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca
    25021 tcaaacagcg ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc
    25081 cccagggctt ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg
    25141 atttgcgcgc tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc
    25201 ggaaattgca cgaactcttc acggagcagg cgacgcgtct cttctgccgc caggattgaa
    25261 gaacgctgcc gttgcggatg atttttcgcc aggctcacga tatcgtgaaa ataacaggcg
    25321 gttaaaatca ccagcatatc gacatcgtca tctgccgcca gtttctgtgc cgtagcccag
    25381 acacggcgaa aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac
    25441 cagttctcga actatgcctg ccagtgttgt aactccatac gcgctccgtt gccaaagagt
    25501 ctcaacctta gcagttttta ttcatagcgt ctgcaacgtg cgggacgagg tcgggtgtac
    25561 catgcaatgc cgcctaacaa ggcaccaacc agggccataa acagaaatcc gaccaatgct
    25621 cctaaccact tccccgcggt agatcctaaa tcactttcaa atcccgacac gggggcatcg
    25681 gggatccggc tcatcaccca gatgtatcgc gccattgccc agacgaaata gccacaccat
    25741 gta
//
LOCUS       DAJTRN010000057        25171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRN010000057 DAJTRN010000000
VERSION     DAJTRN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..238
                     /locus_tag="OO328_002389"
     CDS             <1..238
                     /locus_tag="OO328_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR5930925.1"
                     /translation="RVLSRGTALAGTGEAAMTGEQILATHRSGKTEVYQRQAGFITGP
                     AKVLMLTLTTQRPFDDHTDQLWTAWLTSFQPAKS"
     gene            260..667
                     /locus_tag="OO328_002390"
     CDS             260..667
                     /locus_tag="OO328_002390"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5930926.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            664..2952
                     /locus_tag="OO328_002391"
     CDS             664..2952
                     /locus_tag="OO328_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5930927.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            2949..3938
                     /locus_tag="OO328_002392"
     CDS             2949..3938
                     /locus_tag="OO328_002392"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930928.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            4038..4241
                     /locus_tag="OO328_002393"
     CDS             4038..4241
                     /locus_tag="OO328_002393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930929.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            4259..4579
                     /locus_tag="OO328_002394"
     CDS             4259..4579
                     /locus_tag="OO328_002394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5930930.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(4645..4877)
                     /locus_tag="OO328_002395"
                     /pseudo
     CDS             complement(4645..4877)
                     /locus_tag="OO328_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(4987..5757)
                     /gene="yafV"
                     /locus_tag="OO328_002396"
     CDS             complement(4987..5757)
                     /gene="yafV"
                     /locus_tag="OO328_002396"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5930931.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            5938..6384
                     /gene="ivy"
                     /locus_tag="OO328_002397"
     CDS             5938..6384
                     /gene="ivy"
                     /locus_tag="OO328_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5930932.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(6427..8871)
                     /gene="fadE"
                     /locus_tag="OO328_002398"
     CDS             complement(6427..8871)
                     /gene="fadE"
                     /locus_tag="OO328_002398"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5930933.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            9111..9689
                     /gene="lpcA"
                     /locus_tag="OO328_002399"
     CDS             9111..9689
                     /gene="lpcA"
                     /locus_tag="OO328_002399"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5930934.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9895..10662
                     /locus_tag="OO328_002400"
     CDS             9895..10662
                     /locus_tag="OO328_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5930935.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(10633..11373)
                     /locus_tag="OO328_002401"
     CDS             complement(10633..11373)
                     /locus_tag="OO328_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5930936.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            11757..12278
                     /locus_tag="OO328_002402"
                     /pseudo
     CDS             11757..12278
                     /locus_tag="OO328_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            12454..12951
                     /gene="rayT"
                     /locus_tag="OO328_002403"
     CDS             12454..12951
                     /gene="rayT"
                     /locus_tag="OO328_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5930937.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(13168..14880)
                     /locus_tag="OO328_002404"
                     /pseudo
     CDS             complement(13168..14880)
                     /locus_tag="OO328_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            14852..15637
                     /gene="lafU"
                     /locus_tag="OO328_002405"
     CDS             14852..15637
                     /gene="lafU"
                     /locus_tag="OO328_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5930938.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            15708..16763
                     /gene="dinB"
                     /locus_tag="OO328_002406"
     CDS             15708..16763
                     /gene="dinB"
                     /locus_tag="OO328_002406"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5930939.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            16815..17108
                     /gene="yafN"
                     /locus_tag="OO328_002407"
     CDS             16815..17108
                     /gene="yafN"
                     /locus_tag="OO328_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5930940.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            17111..17509
                     /gene="yafO"
                     /locus_tag="OO328_002408"
     CDS             17111..17509
                     /gene="yafO"
                     /locus_tag="OO328_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5930941.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            17519..17971
                     /locus_tag="OO328_002409"
     CDS             17519..17971
                     /locus_tag="OO328_002409"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5930942.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            18217..19260
                     /locus_tag="OO328_002410"
     CDS             18217..19260
                     /locus_tag="OO328_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5930943.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            19322..19936
                     /locus_tag="OO328_002411"
                     /pseudo
     CDS             19322..19936
                     /locus_tag="OO328_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(19993..21450)
                     /gene="pepD"
                     /locus_tag="OO328_002412"
     CDS             complement(19993..21450)
                     /gene="pepD"
                     /locus_tag="OO328_002412"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5930944.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            21712..22170
                     /gene="gpt"
                     /locus_tag="OO328_002413"
     CDS             21712..22170
                     /gene="gpt"
                     /locus_tag="OO328_002413"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5930945.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            22262..23506
                     /gene="frsA"
                     /locus_tag="OO328_002414"
     CDS             22262..23506
                     /gene="frsA"
                     /locus_tag="OO328_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5930946.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            23564..23965
                     /gene="crl"
                     /locus_tag="OO328_002415"
     CDS             23564..23965
                     /gene="crl"
                     /locus_tag="OO328_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5930947.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(24004..25059)
                     /gene="phoE"
                     /locus_tag="OO328_002416"
     CDS             complement(24004..25059)
                     /gene="phoE"
                     /locus_tag="OO328_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5930948.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         6127 a         6373 c         6686 g         5985 t
ORIGIN      
        1 ccgggtactt tcgcggggaa cggcactggc gggaactggc gaagctgcca tgacaggtga
       61 acagattctc gcaactcatc gttctggaaa aacagaagtg taccagcgtc aggccgggtt
      121 tatcaccggg ccagcaaagg tactaatgtt gacgctgacc actcagcgtc cttttgatga
      181 tcacaccgac cagctatgga ctgcatggtt gactagtttt cagcctgcta aaagctaaaa
      241 aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg gatgacccca tctaccacac
      301 cagcgccctt gccgggtttt tgataggggc catcataggt atcgccatta ttgccgtcgc
      361 cagctttgct ttttttacct gcggcttttt ggccgggctg attttgagtt ttctggctga
      421 tcagatagct tccggagtcc tgcaactggg tgaggctatt gggcgctcga tacattccac
      481 agtgggaaaa attctgaccg gttctgataa tgtcagcacc aacagtcgcc cggccgcccg
      541 ggccgtactg agtacggtga tatgcgaaga ccattccccg gaaatccgca ttgcccaagg
      601 ttccggcaac atctacatca acagccagct gcccgtaagg atgaccacac tgagtgtgat
      661 gcagtgattg aggacggctc ccccaatgtc tttctcggcg gcggtacgca gacggtactg
      721 gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg tggatgtgct gtatgtggtc
      781 gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg cggcaaagat ggggtcgaaa
      841 tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa tggtcgggat ggccatctct
      901 gacacagtca tgggattgtt cagcaatccg gtagatgtca ccactgggca aaaaattttg
      961 ctgccggaga cggattttac cctgccagga cgcctgcctg tcacctgttc gcgcttttac
     1021 gccagccacc tggaaactga aggattgctg ggcagaggat ggcggctgaa ctgggagata
     1081 aacctgcgtg aggatgaaac atatatcacc ttcatcggtg tacagggtcg ggaactgagt
     1141 tatccgaaag agatgctgat accgggccac cagatattcg acccggaaga gcagttttac
     1201 ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca ccgactgcag ctattacgtg
     1261 tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt ttatggagac gccgtaccgg
     1321 cagcgtattg ctttcgggcg tgagaacggc aggctggtgc gggtggcctc cagcagcgga
     1381 caccatctgt tattgcaccg cacgatgacg caagcagggg aacgcctgtc gcatattgaa
     1441 cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc gttacgacga taacgggcaa
     1501 ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc agtttgccta tgaaaatggc
     1561 ctgatgacag agcatcgcaa cgcgacgggc tttacctgta cctaccactg ggaagagatt
     1621 gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg gcgaggatta ccggttccac
     1681 tatgattttg ctggcgggca gacggtggta accggcaggc cggagcagaa gtggcagtgg
     1741 tggtttgatg aagagacgta tgtgactgcg caccggacgc cgggcggcgg aatgtaccgc
     1801 ttcacgtaca acgaaaacca ttttccggta gcggtggaac tccccggcga acgcagggta
     1861 acgctggaat acgacaccct gagccgggtg gtgaaagaga cggacccggc aggccgggtg
     1921 acacagacgc agtggaacgg cagttttgct gaaatcacgc gccgggcgct ggatgacgac
     1981 cacgtatgga aggcggatta caacgaacac ggccaggtga tacgggagac ggacccggaa
     2041 gggcgcgtca cgcggtacgg gtatgatgat caggggctgc cggagacggt ctgccacccc
     2101 ggtaaacagc aggaccgtta cacctggaac gcgctggggt tactgagcag ccaccgtcgc
     2161 atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg ggatgctggc gcgccatacg
     2221 gatgaagaga aacgggagac gcgctggcag tacaccccgg aagggctggt tgccagtctg
     2281 agcaacggca acggggctca gtaccggttc agctacgacg gcgacggacg gctgacggga
     2341 gaacagcgcc cggacggcct gataaggatg tttgcgctga atgcggacgg gtttccggtc
     2401 atcatcccga cgcagggcac agagggcggg gtgcgcaatg aacagcagga gcgtgatgcg
     2461 ctgggccgcc tgctgcgcag tgacacgcag cacagtacac ggaccttcag ctacaaccgt
     2521 cttgaccaga taacggaagt gacgctcacc cccacggaag aaggggaacg gctgcaccac
     2581 atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg ggtggctgac ggcagagcac
     2641 agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg gcaatcccac cgatatcacg
     2701 ttgccggacg ggcagcatct cagtcatctt tactacggca gcgggcatct gctccagacg
     2761 gcactggacg gtatcacggt cagtgaatat gagcgtgaca gcctgcaccg tcaggtgata
     2821 cgcacgcagg ggaaactggc gacgttcagc ggctacaacg ccgacaaccg gttaagctgg
     2881 cagcgcagcc tgccaggtgg cagccagaat ccacagcagg cggtactccc ccggcgcaga
     2941 acgacagcgt gacggcgcgg gactatatct ggaacgcaga cggtgaagtc ggcggcatca
     3001 atgacaaact gcgcggttgc ctggtgttca gctatgaccg cagcggctgg ctgaccagcc
     3061 gcacggggca gatgtatgac catgaccatt attattacga caaagcgggc aacctgctga
     3121 cggatgagta tcagggagcg gtgatggata accgcctgcc gggttacggg cgtgaccgct
     3181 accgctataa cgaatggggc gaactgacag agcgcaggga ccagcagctg gagtggaacg
     3241 cgcagggcca gttaacgcgg gtcatcagca gcaacagcga gacgcggtat cagtacgatg
     3301 cgctgggcag gcgcatcagc aaggcgacca gcaatctcca taccgaccgg ggagaacgaa
     3361 gccggacaac gtttgtgtgg gaagggttcc ggctgttaca ggagacaacg tggcagggaa
     3421 agcggacata cctgtacgat gcggaacagc cgtacacgcc ggtggctgcc atcacggggc
     3481 gcggagagag ccagaaaatc tggtattacc acacggacct gacgggcacg gtacacgagg
     3541 taaccgcgcc ggacgggacg ctggtgtggg cgggctacca ggcgggattt ggtgaaaacc
     3601 ggggcgacat cagcaacagc ggggcgtact ttgagcagcc gcagcggctg ccggggcagt
     3661 actttgacga agaaacaggg ctgcattaca atctgttcag gtattatgcg ccggagtgcg
     3721 ggaggttcgt aagccaggac ccgataggat taaacggtgg gttgaactta tatgcttatg
     3781 cgcctaatcc gttaggttgg atcgatccgc taggactgaa ttctttagga atagattcaa
     3841 ataatatatt tagaggagat ggtaactata aaggtggcgg aattggacag ccaaagagtg
     3901 gcacaatatc agcacaggac attattgatc atctctaaga aaaaggtgat gggcgtttaa
     3961 catcttttag tactaaaaaa acatctagtt ccaactctcg tggtgcagat tttttctcaa
     4021 aaaaacatgt cttcaaagtg gcagtttctg atctcgatga tttagttaaa aataatcaga
     4081 tcactgtttt aacacaagaa aaagtaaaag caatatttgg tttagaatca agcaagaaaa
     4141 ttagaaaaga acttggtaat attcttcaga atatggctag gaataatgag gtgttagttc
     4201 atgggcaaat tcttggaaat aaagttagcc gatgtaatta gttagtggag aggaagttgt
     4261 ggacgaaatt agaataagaa ttgctggtaa tacggctgcg ccttgtttcc atgtgctaaa
     4321 aaataaggga tataaagtaa caatggaaac tcatgcatta tctgacgatt gtttagattg
     4381 tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt agtgcaacga gtccagaaga
     4441 gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat gactggagag ctaatttgaa
     4501 tgagataaat acttatcaaa aagccataga agaatccccg ttttttgata aaaacggcaa
     4561 tgaaatagag tctgactaaa ttttattgat atagcaacga taataaccgg aaatttcttc
     4621 cagatctttc cggcttttta ttatttaact ttcgttcagc acactaaaca tcccgtctcg
     4681 catcccctta actggctgtt taacctgata aagttccctg cgcagttcct gtacttcgtt
     4741 gttatcccaa gcaggatcaa acatccctcc gagatcttca cggtaacgcc cgtgccgatc
     4801 tcaaatcccg cttttctcag ccatttggcg ctgattttga tcaccggcgt gtcgctgcgg
     4861 ttgggcagat aacccacggt acagcgacgc tgtgttttgg aaatggtgac ttctggcgta
     4921 atattgtgtt taagttaggg gctttcgtaa tctagcccag cccccggccc taaacacaac
     4981 ccccactcac cacaacctaa actcatccgc atcccgccat gccggaaact tttctctata
     5041 ttcccgcaat gccaccatcg acagctccgc atcaatacgc gttgccttat gcgcgtcggc
     5101 ggtagcgata atctcgcctt gcggattaat cacccggctg tcaccgcgat aatggcagcc
     5161 gttgccatcg ctgccgacgc gattgcatcc cgccacatac gcctgattct caatcgcgcg
     5221 ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct ggccagttgg cgacgtacag
     5281 ggcgaggtca taatcgttga gattgcgcga ccacactgga aaacgtaagt cgtagcacac
     5341 cagcggcaaa atacgccagc cgcgccattc cacaatcact cgcgcattgc ccgctttata
     5401 atgcagatgc tcatctgcca tgcggaacag atgacgctta tcataaaaat gtaccgtgcc
     5461 gcccggctca accagcaaaa agcggttaac cgaaccagac tccgtttgta atgcaacact
     5521 gcctgcaatc agcgcattgc actgctgcgc cttagctgtc atccagttca ctacgtcatc
     5581 ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg ctggtaaaca tctccggtag
     5641 aacgatcaca tcgcgcccgg taataccttc cagttgacga tcaaaatggc gcaggttggc
     5701 aggaccatcc acccacacca acggttgctg caaaagcgta atcttcaaac taggcacggt
     5761 gtacaactcc tttatgcgaa gggttttata actttaacac cttatcaggc agttgcctta
     5821 gcgcagaata aattgataac aaatgctgat attggaaata tctgatttgc aaattatcgt
     5881 gttatcgcta ggctttagga ggttaataac atgggcagga taagctcggg aggaatgatg
     5941 tttaaggcaa taacgacagt cgccgcactg gtcatcgcca ccagtgcaat ggcgcaggat
     6001 gatttaacca ttagcagcct tgcaaagggc gaaaccacca aagctgcgtt taatcagatg
     6061 gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg gtacttatac tcccgcacaa
     6121 accgtaacgt tgggagatga gacgtatcag gtgatgagcg cgtgcaaacc gcatgactgt
     6181 ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta atcagatgac ggggctgttc
     6241 tcggctattg atgagaaaac gtcgcaagag aaactcacct ggctgaatgt gaacgatgcg
     6301 ctttcgattg atggtaaaac ggtgctgttc gcggcgttga ccggcagcct ggaaaaccat
     6361 ccggatggct ttaattttaa ataattagcg gataaagaaa cggagccttt cggctccgtt
     6421 attcaattac gcggcttcaa ctttccgcac tttctccggc aactttaccg gcttcgtcgc
     6481 cagctcttcc ggatcaaagt catcaacgtt aatactgcgc agacggcttt cttccgcttt
     6541 caccagaata gcggcttcat ctttatcaat cagccccttc gtcagcgcgt tgtgcgccag
     6601 ttcatccaga cgggtaaatg gcagattttt acccagctct ttacagatcc gctgatgaat
     6661 cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt aaaccaaccg ggttatgctc
     6721 gcttggcgtc aggtactgac cgcgaccaat acgtgaacga gtggcgttcg gcacttgtaa
     6781 aatcttcgcc attttatgat ccagcttgtc agacggtgcc agataatgac gtccggtcgg
     6841 gaagatcacc acattcagca gcccggcaac cacgcggttc gggaagtttt gcagtaaatt
     6901 gtccattgcc tgttctgcct gatacagcgc atcctgtacg ccccagtgca ccagcggcag
     6961 atcggcttca ttacggcctt cgtcgtcata acgcttcaga acggcagagg cgaggtaaag
     7021 ctggcttaaa atatccccca gacgtgccga gatacgctca cgacgtttca ggctgccgcc
     7081 cagcagtgcc atcgagacat cagaaagcag ggcgaggttg gcgctcaggc ggttcaggtg
     7141 ctgatagtag cgtttagtgg catcgccggt tggcgtgctg ctggttaaac cgcgcgtcag
     7201 gcccagccag aagctgcgaa ctttgttgct accgacgtga ccgatatgtt tgaacaacag
     7261 tttatcgaac gcgttgacgt cattgttctt cgccgcttcc atctcttcca gcacgtacgg
     7321 atggcaacga atcgctcctt gtccgaagat catcatgctg cgggtcagaa tgttagcccc
     7381 ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc gccaggaagt tgctttgccc
     7441 gagcataatg cctttaccgc cggtaatatc catcgcatca ataatcgact gctgcccgcg
     7501 gtgggtacag tgatacttaa cgatagccga cagcacggca ggtttttcgc cgagcataat
     7561 gccgtaggta atcagcgatg ccgcagcatc cattacataa gcgttcccgg caatacgcgc
     7621 cagcggctct tcaatccctt ccatcttacc gatagagatt ttgaactgac ggcgaatgtg
     7681 agaatacgcg cccgttgcca gcgctaccga tttcacgccg ccggttgagt tggaaggcag
     7741 ggtgatgccg cggcctaccg agaggcactc caccagcatc cgccagcctt gcccggccat
     7801 tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca tctttaccgc gcgtcggtcc
     7861 gttctggaac ggtacgttca gcgggaagtg gcgacgacca atttccacgc ccggcgtggt
     7921 ggttgggatc agcgcacagg taatgcctaa atcttctgca ccgccgagta atttttccgg
     7981 gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc ggtgccagcg taatgtagcg
     8041 tttgttccag gtcagacgca tccccagcac ctgctggccc tgccattcgc ccatgcagac
     8101 aatcccggtg tccggaatcg cgcccgcatc ggaacccgct tccgggctgg tcagtgcaaa
     8161 gcaggggatc tcctgaccac gcgccagacg cggcagatag tgatctttct gctcgtcagt
     8221 gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt ggcacgccga cggtaatcgc
     8281 caggatcccg ctcacgccag agagtttttg cagcacacga gactgggcat aagctgagaa
     8341 ctccagtccg ccgtactctt ttttgataat catcgcgaag aagcgatgct ctttaaggta
     8401 cgcccacaac tccggtggca gatccgccag ctcatgagta atctggaaat cattcgccat
     8461 ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc tcttcagcgg tcaggcgcgg
     8521 ctgcggatag ttatgcagct ttttccagtc cggcttgccc tggaacaagt cgccctccca
     8581 ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc gacatcggcg gcatcacctt
     8641 acggaaaccg cgaaataccg gcgcggaaat catcgactta cgcataggcg caaagttaaa
     8701 tggcacgagg ataatggtca gaggcaccag tacccacgcc gaccacagac cagcaacgcc
     8761 gagggcggct gtccaggcga gcaaaatcag actgctgata aataagctca cgcggtgata
     8821 gaacaacgcg ccgagcagga caaccgtagc gagaatactc aaaatcatca taacgaaaag
     8881 ccccttactt gtaggaggtc tgaccacttg tgatgatatg gttgtagtgg atgtaaaaac
     8941 atttagcaat gtgtttacaa tataattaca acaaagctca cattgttgct gtttttatcc
     9001 gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg tacttctcgc ttttggcggt
     9061 atccggtaca ctgcattttg tctattacat ttatgctgaa ggatatcctc atgtaccagg
     9121 atcttattcg taacgaactg aacgaagcgg cggaaacgct ggctaacttt ttaaaagatg
     9181 acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt agcagacagc tttaaagccg
     9241 gtggcaaagt gctttcctgc ggcaacggcg gttcccattg cgacgctatg cactttgccg
     9301 aagagttgac cggtcgctac cgtgaaaacc gtccgggcta cccggcgatt gctatttctg
     9361 acgttagtca tatttcctgc gtcggtaatg atttcggttt caatgatatt ttctcccgct
     9421 acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg gatctccacc tccggtaact
     9481 ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa ggggatgaaa gtgatcaccc
     9541 tgaccggtaa agacggcggt aaaatggctg gcacggcgga tatcgaaatt cgcgttccgc
     9601 actttggtta tgccgaccgc attcaggaga ttcacattaa agtgatccat atcctgatcc
     9661 agttaattga aaaagagatg gttaagtaag tctggcgtag gccggataag gcgtttacgc
     9721 cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg acgcgtctta tcatgcctac
     9781 aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg ccgcatccga cctgaaaatt
     9841 cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt tgtggaggtt acccatgtgc
     9901 gaattgctcg ggatgagcgc caacgtccct accgatatct gctttagttt caccggactt
     9961 gtacagcgtg gtggtggaac cgggccacat aaagatggct ggggcattac cttttacgaa
    10021 ggtaaaggct gtcgcacatt taaagatcca caacccagct ttaattcccc catcgccaaa
    10081 cttgtccagg actacccgat aaaatcctgt tcggtggtgg ctcatattcg ccaggctaat
    10141 cggggcgagg tggcgctgga aaatactcac ccatttaccc gcgagttatg ggggcgtaac
    10201 tggacttatg cccataacgg acaactgacg ggctacaaat cactggaaac cggcaacttc
    10261 cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct ggctgctgca taaattaacg
    10321 cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt ttaaatatat cgcctcactg
    10381 gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc tttcggacgg gcgctatgta
    10441 atggcgtatt gctcgactaa tttacactgg atcacccgcc gcgcgccgtt tggcgtggca
    10501 acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc agaccacacc gaatgatgtg
    10561 gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa cctggcaaaa aattatgcca
    10621 ggcgaatggc gcttattttg cctcggggag cgtgtagttt gatgccagtt gtggctgcac
    10681 cacttcgtgg cttaacggct tgctgaccac gtaacggcca ttggccacag aaacggtcgg
    10741 tggcttacgg gtttgctcaa agtagtcgta gcccggcttc agctgctccc agaagtcctt
    10801 aaagttggaa tatttatggc gcttcatatt ggcgtcggtc atgcggaatg ggtaaatact
    10861 cacctgcacg ctcggctgac caaacaccag cgcaccagta acgaactgga agatctcatc
    10921 aataccctga ttggtcattg cgtagcagcc gatggataca caatcgccgt ggatcatcag
    10981 gtatttccct tcataaccat gcgcacggtc ataagcattg gggaatccaa tattaatcgc
    11041 tttgtagtaa cggctgtctg gttttaactg attacgctgg acgctataaa acccttccgg
    11101 gcttttgaaa tcgccctgac gctgttttgg ccctaagccg ccggaatatt tacagatttt
    11161 ataactgtcg agcagttgat attgctcgcc cattttgacg taaagatcga gcgtacgttc
    11221 ttccttgaag atctggatgt agacagggga tcccatcaac tgctgcttat actctttgct
    11281 cactggcgtg gtcgagctac tgctgcccag caaaccggca aacgaaacgc acgggatcaa
    11341 caacatcgca agaattaatg cgattttacg catactgctt attccttgat aaaacggtta
    11401 cacacgccag gacggcaaaa tggatcccaa atcggaatag tctggatttg gaaggctcac
    11461 attatcacca aaagagtttt acgcaagcct gtcggcgctg ggtttacaaa tttagcacag
    11521 gggcagtttt caatagtccg aaaagcgcat gtatcattta tttcctaata ttatgaaatc
    11581 tcaatagtaa tatgcaaata agcaattaat aatcccgttt actcctccgg tcttttaaaa
    11641 ttctcacctt cattcagccg gtttatgtct ttcatgtctt cgttcttgct cggcagattt
    11701 ttgcatccag gcgtgttttc gttatgcgtt ttgctccctt tactcgccag cgccaccaca
    11761 accgggagca gataaaacag catccagaat ggtttcctgc gccgctcaag gcgagtgaca
    11821 gacgctggca ggcgctggcg gaaaacaacc actttttaag cagcgatcac ctgcataaca
    11881 tcaccgaagt ggcgattcac cgcctggagc agcagcttgg caagccttac gtctggggcg
    11941 gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt tttttatgcc tacaacaaga
    12001 tccttgaggc gaaactcccg cgcacggcca atgagatgta ccactaccgt cgggcaacga
    12061 ttgtggcgaa taacgacctg cgccggggag atttgctatt tttccatatc cacagccgcg
    12121 agattgccga ccatatgggg gtgtatctgg gcgacggtca gtttattgag tcactgcgca
    12181 ccggggaaac cattcgggta agccggttag ccgaaccttt ctggcaggac cattttttag
    12241 gcgcgcgcag gattttgacg gaagagatga ttttgtaggc cggataaggc gtttacgccg
    12301 catccggcgg ttgtgcacgg atgcctgatg cgacgctgac gcgtcttatc atgcctacaa
    12361 atctgtgcgt ttttgcgagc cgctttcccg atataacaca gcgtcattgt cggcctttcc
    12421 ttttcctctt accgttgaga aaaaggagtc gctatgtcgg aatatcgtcg ttattacatc
    12481 aaggggggaa catggttttt cacggtaaat ttacgcaatc gtcgaagcca tcttctgacc
    12541 acccagttcc agacgctccg taacgccatt attaacgtta agcgagacag accttttgaa
    12601 attaacgcct gggtcgtttt gccggagcat atgcattgca tatggacatt acccgaaagc
    12661 gatgatgatt tttcctctcg ctggcgggag attaaaaagc agttcaccca tgcttgcgga
    12721 ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca ttcgcaatac aaaagattat
    12781 cgccatcatg ttgattatat ttatataaat ccagagaaac atggctgggt aaagcaggtg
    12841 agtgattggc cattctcaac gttccatcgc gatgtcgcgc gagggttata tcccatcgat
    12901 tgggcgggag acataacgga tttaagtgcc ggggagcgta tcattttata actgtgcgct
    12961 ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt gctttaacgg taggccggat
    13021 aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc tgatgcgacg ctggcgcgtc
    13081 ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga taaggcgttt acgccgcatc
    13141 cggcaattgt gcaccgatgc cggattctta catcaacgcc cccataatct tcaactccag
    13201 ctcatccggc acttcgttat acgacagcac atgcagcccc ggcgcaaaca accttgcata
    13261 acgcggcagc aaagggcgta gctgcggcgg caccagcagc accggatctt tccccgccgc
    13321 tttcatctgc tctttcacct gtggcatcgt gctctggaac tggttgagca tattcgggtc
    13381 aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc gcctgattca ctacgttggt
    13441 cagcagattt tccagctcat tattcagcgt atacaccgtc agctcctgct tgcgaacgaa
    13501 cggatgagta atgctgcgcc gcagcgccag gcgcacatcg gccgccagca gaatatgatc
    13561 tttagtcacc gtgctactgg cgaccagcac ggtggcgatg gtgacgatat cgcgcaggga
    13621 aacgccttcg gtcagcagcg cccggtacac tttcagcaat tggctgtaat tgagcgccgc
    13681 gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa cggttatgca actgcgtgat
    13741 gtcatcatag ttaaacaaat caggaatata gctgcgcaca atcttgttca catgggtggc
    13801 aatcacactg gcgctgtcga tcacctgata ccccatattc agagccttcg ctttctgcgc
    13861 aggctggatc caggtgaccg gtatcccgta cgccgggtcg ttccccagca cgccgtcaat
    13921 ctcgccgtag gtttcgctgg agggcagcgc catcagttta tccgccggaa tatccgcttc
    13981 atcagcctca atgccgttga tgaaaatagc gtactggctg ggcttgaggc ggaagttttc
    14041 ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg gaaatcacct gccgaacgcc
    14101 gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc accagcgcca ccagcttgta
    14161 gccgaggctt aagctgatgg gctcgatcag cggaatagtt tcccaactga cctgttgttc
    14221 gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt ttctcttccg cctccgccgc
    14281 ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc agggtgctga acagcaaaaa
    14341 cggcagatgc ggcatccccg gcaccaccgc cagcacgaac atgatcccgg tggcggtgta
    14401 gagcaccgac gggcttgcca gcaactggtg gcgcacgtca tgggcgatat cgccgttgtc
    14461 gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc agcagggaag ggatctgcgc
    14521 caccaggccg tcgccgatgg tcatcagcac gtattgctgg aaggcggcgt cggcgctcag
    14581 gttgtatttg aagatgccga tacagacgcc gccgctcagg ttgatcgcca gaatcatcat
    14641 cccggcgatg gcgtctccgc gtacaaactt cgatgccccg tccatcgcgc cgtagaaatc
    14701 ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc gcctggttga tcaatccggc
    14761 gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc gcgtccaggg taaagcgggc
    14821 agaaacctcg gaaatacgct cggcctcttt ggtgacgacg ataaagttga ttgtcaacag
    14881 cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg cacgggcagt cgatttttaa
    14941 cggtggtggg ctgtcgccgt taaataaaat cagcccgtct catccgccaa aacctgccac
    15001 cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat gtgaatgaga aaacggcgct
    15061 gctgaagaag aaatccgcca ccgagcttgg cgagttggca accagtatca acaccattgc
    15121 ccgtgatgcg catatggaag cgaatctgga gatggagatt gttccccagg gattacgcgt
    15181 gctgattaaa gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc agattatgcc
    15241 gttctttaaa actttgctgg tggagctcgc gccagtgttc gactcgctct ataacaaaat
    15301 tattattacc gggcataccg atgcgatggc ctacaaaaac aatatctaca acaactggaa
    15361 cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg gaagaggccg gaatgccgga
    15421 agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggacg cgaagaatcc
    15481 gcaaagcgcg ggcaatcggc gtattgagat tatggtgctg accaaaagtg cgtccgatac
    15541 gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg tgcaaaagct
    15601 ggataagcag caggtgcttt cgcagcgaat gcgctaaatg ctgaatcttt acgcatttct
    15661 caaaacctga aatcactgta tactttacca gtgttgagag gtgagcaatg cgtaaaatca
    15721 ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat cccgctctgc
    15781 gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc agcaccgcca
    15841 attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc tactgggatg gcgctcaaat
    15901 tatgcccaca tctcaccttg cttccggggc gctttgacgc ctacaaagaa gcctcaaatc
    15961 atatccgtga aatcttctcg cgctacacct cgcgcattga accgttgtca ctggatgagg
    16021 cttatctcga tgtcaccgat agcgtccatt gccacggttc tgcgaccctc atcgcccagg
    16081 aaatccgcca gacgatcttc aacgagctgc aactgacggc atctgcgggc gtggcaccag
    16141 taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggtcag tttgtgatta
    16201 cgccggcaga agttccggca tttttacaaa ccttaccact ggaaaaaatc cccggcgtcg
    16261 gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg gacctgcggt gatgtacaaa
    16321 agtgtgatct ggtgattctg cttaaacgct ttggcaaatt tggccgcatt ttgtgggagc
    16381 gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttacgaaaa tccgtcggcg
    16441 tggaacgcac gatggcggaa gatattcatc actggtctga atgtgaagcg attatcgagc
    16501 ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa acctgattta ctgattgctc
    16561 gccagggggt gaaattaaag ttcgacgatt ttcagcagac cacccaggag cacgtctggc
    16621 cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat gaacgccgcg
    16681 gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt gcttgacccg caaatggaaa
    16741 gacaactggt gctgggatta tgatgtatac tattatgtat attctggtgt gcattattat
    16801 gagggtatca ctgtatgcat cgaattctcg ctgaaaaatc ggtcaatatc actgagttac
    16861 gtaaaaaccc agctaaatac tttattgatc aaccagttgc ggttctttct aataatcgcc
    16921 ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt aatggacatg cttgctgaac
    16981 aagaggagaa aaagcccata aaggcgcgct ttcgtccaag tgctgcaaga ttagaggaaa
    17041 ttacacgccg cgctaaacaa tatcttaatg atatgacgga tgatgatttc aatgacttta
    17101 aggaataagg atgcgggtat tcaaaacaaa acttattcgc ctgcaactta cagcagagga
    17161 acttgatgcg ttaacggcgg attttatttc ctataagcgt gacggtgttt tgccagatat
    17221 atttggtcgc gatgcactct acgacgactc gtttacctgg ccattaatca aatttgagcg
    17281 agttgctcat attcatctgg caaatgtgaa taatccattt ccgccacagt tgcgccaatt
    17341 cagcagaacg aatgacgaag cgcatttggt atattgtcag ggtgcgtttg atgagcaagc
    17401 atggttgctc attgccattc tgaagcctga acctcataaa ctggctcgag ataacaacca
    17461 aatgcataaa attggaaaaa tggcagaagc gtttcgcatg cgtttttgaa tttatattat
    17521 gaataacata caaataagag attatcagcc tggcgatttt cagcaactat gcgctatttt
    17581 cattagagcg gttacgatga ccgtcagtca gcattattca ctacaacaaa ttgccgcctg
    17641 ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc
    17701 agttattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata tcgatatgtt
    17761 atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa aaccttttat
    17821 taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct tttttgaacg
    17881 ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt ggtttattaa
    17941 tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgc
    18001 ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat gacaaatcct
    18061 ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg ccctggattc
    18121 gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc agcacatgca
    18181 acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat tcgtccctta
    18241 tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt agcgatccaa
    18301 caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat gccagattta
    18361 caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg tttttaccca
    18421 gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga tattctcgct
    18481 cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga cgttgctgaa
    18541 gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg
    18601 cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga tcaaatcatc
    18661 aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact gctggttcat
    18721 agcggctcga gggggctagg gcagtctatt ttacagcggc atattgcctc gttttcgcat
    18781 catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa tcgccacctg
    18841 atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct ggatgtggcg
    18901 cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca tcgtaaaggt
    18961 gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga ttactccagg
    19021 ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc tggcgcatgg ggctgggcgt
    19081 aaatgggggc gcaccgagtg taaagggcgt ctggcagcga aatacacagc gacgcaactc
    19141 tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata aacaactcat cttcgaagaa
    19201 gcgccacaag cctataaatc ggctgaaagt gtggtgcaat gtcagggcag gccggattaa
    19261 ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa aaacagtgga gggaaaaaag
    19321 gatgatcttg ctataactct cctcagcgca ggggccggaa gaatgttgtc tcgcagtgaa
    19381 aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa gacgtcgcgg taacggtgct
    19441 ggaaacagaa acaggccgct actctgacac gctgcgttcg gcgctgattt ctctggatgg
    19501 cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact attcagtgga tttgcccgag
    19561 cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg ggcattgggc gttttaccgc
    19621 tgatgagcag gagcaatccg atgcgatccg gtatgagacg ctgcgttcgt cggggccggg
    19681 cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat
    19741 tagcgtgaag gttcagtcag agcgcagcca gcatgctaac aaacggctgg cgcgattgct
    19801 gattgcctgg aagctggatc agcagcaata ggaaaatagc gcggtgctga aatcacagcg
    19861 gcgaatgttc catcaccaga ttgaacgtgg caacccgcga cgaacgttta cagggatggc
    19921 ttttatcgaa ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag
    19981 caaatcaaat aattacttcg ccggaatttc tttcagcagt tcagtcagca gtgtccagta
    20041 atgacctacg ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgatagttgg
    20101 cccgatagaa accatgtcca tttccggata cggttttttg aacagaccac attccaggcc
    20161 cgcgtggata atctggatgt tcggcgtctt gttgaacagg cgctgatagg tttcacgtac
    20221 cagatgcatc accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc
    20281 ggttttcgcg ccagccagtt tacccagcga atccagcatg ctcaccacgt agtctttacc
    20341 gctgtcgatt agtgaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac
    20401 cacaccgacg ttcagggagg tttcgaccac accttttgcc acgtcggaat tgcggatcac
    20461 accgttcggg gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc
    20521 agctttatcg ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag
    20581 ctcgtttttc aggatctcct gataggtatt caccagagat ttcagtgcgt cgactttatc
    20641 agctgcgaca gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc
    20701 gttgaaatcg ataaggcgca ggtccagttc ttctgcatga cccgccagga agcgcaccag
    20761 cagtttgttg gcattaccca ggccaacgtg gatttcaccg ccggagtgac cgcctttcag
    20821 accttttaag gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg
    20881 caggttggag gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc
    20941 ggagtcggtg ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc
    21001 gtccataccg gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt
    21061 ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg ccgttatccg cacccagcgt
    21121 ggtgccgcgc gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa
    21181 gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac
    21241 cggtttacga ttttccatac ctgcggtagc aggttgacga atcaggatat tacctacctg
    21301 atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg
    21361 ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg
    21421 ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa
    21481 cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga
    21541 tatgtattga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
    21601 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
    21661 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
    21721 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
    21781 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggg
    21841 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
    21901 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
    21961 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg ctgttgcgat tcgtgaaatg
    22021 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
    22081 gactatgttg ttgatatccc gcaagatacc tggatcgaac agccgtggga tatgggcgtc
    22141 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcatt gccgggcgtt
    22201 gttcttttta acttcaggca ggttacaata gtttccagta agtattctgg aggctgcatc
    22261 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
    22321 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga
    22381 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
    22441 tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgctgaacg
    22501 taccgacgat gatttatacg ataccgtgat tggctaccgt ggtggcaact ggatttatga
    22561 gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt acggaagaag atccgcaact
    22621 cagtggtcga cactggctgc atgcggctac gttgtacaac attgccgcct atcctcatct
    22681 gaaaggagat gacctggccg agcaagcgca ggctttatca aaccgcgcct atgaagaggc
    22741 cgcccagcgt ctgccgggca cgatgcggca gatggagttt accgtacccg gcggtgcgcc
    22801 catcaccggc tttttgcata tgccgaaagg cgatggcccg ttcccgacag tattaatgtg
    22861 tggtggtctg gatgcgatgc agacggacta ttacagcctg tatgaacgtt attttgcgcc
    22921 gcgcggcatt gcgatgctga ctattgatat gccgtcggtg ggcttttctt caaaatggaa
    22981 gctcacccag gactccagcc tgttgcatca gcacgtctta aaggcgctgc ctaacgtacc
    23041 gtgggtggat cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt
    23101 gcgtctggca taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt
    23161 tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt
    23221 tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa gcgttacgcg tggagctgaa
    23281 tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc tgcccaacgc caatgttatc
    23341 aggctactgg aagaacgatc cgttcagccc ggaagaggac tcacgcttaa tcacctcatc
    23401 atctgctgac ggtaaattat tagagatccc atttaacccg gtgtatcgga attttgacaa
    23461 aggtcttcag gaaatcaccg gctggatcga aaaacgcttg tgttaaaaat ttgctaaatt
    23521 ttgccaattt ggtaaaacag ttgcatcaca acaggagata gcaatgacgt taccgagtgg
    23581 acacccgaag agcagattga tcaaaaaatt taccgcacta ggcccgtata ttcgtgaagg
    23641 taagtgcgaa gataatcgat tctttttcga ttgtctggct gtatgcgtca acgtgaaacc
    23701 ggcaccggaa gtgcgtgaat tctggggctg gtggatggag ctggaagcgc aggaatcccg
    23761 ttttacctac agctaccagt ttggtctgtt cgataaagca ggcgactgga agagtgttcc
    23821 ggtaaaagat actgaagtgg ttgaacgact ggagcacacc ctgcgtgagt ttcacgagaa
    23881 gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa ccggcgaatg attttcgtga
    23941 tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga tgcatcacat ccggcaatat
    24001 tcattaaaac tgatacgtca tcccaaccgc gacagtatca tcattattaa tattcagctt
    24061 attatcgcta tccagttggt tgattttata atcaacaaac gctgacatat ttttgttgaa
    24121 ataatacgta gcaccgacgt cgatataatt gaccagatct tcatcaccga tgccctcaat
    24181 atctttccct ttcgataaga cataacccag cgatggacgc agaccaaagt caaactggta
    24241 ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag ccgccagtta ttggcgtcat
    24301 tttgcgtgtt tcagaataga aagttgccag ataaatatta ttggcatcgt atttcagacc
    24361 tgttgcccat gcttctgcac gcttgcctgt gccacggctt tgcaggttct gctcgttggt
    24421 gcgatctgag ttggtatagg ccccactaat ggcgaaatcg ctgccgccaa agtcatatgt
    24481 caatgacgtg ccgaagccat cgccgttttg ctttttaacg tcgcggtttt cgtttttccc
    24541 ttgatattgc agggttaagt tcaggccatc gataacgccg aagaagtcgg tgttccgata
    24601 cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg gtctgcgccg aggaatcgcc
    24661 accaaattcc gggaacatat cggtccaggc ttccacgtca tacaacgcgc ccaggttacg
    24721 accataatcg aaagaaccca aatctttata tttcaacccg gcaaaagcga gacgcgtttt
    24781 ttgctgtgca gtatcactct ccgctttatt tccggcaaac tccgcttccc accgaccata
    24841 accagtcagt tgatcgttaa tttgagtttc acctttaaaa ccaaaacgga tataactctg
    24901 gtcgccatct ttactggcgt tatcactcat ataatgcatg gctttaactt tgccatagac
    24961 atccagttta ttaccgtctt tattatatat ttctgcagcc tgtacagatg cagatgccac
    25021 aatgcccatc accactaatg ccagagtgct ctttttcatt ttcattcctg attttaatta
    25081 acgcgcgaat attcagcggg agagccccgt tgaaaacagg aaagttttta acctgagatt
    25141 gttaaagata tattacagat taatgatatt c
//
LOCUS       DAJTRN010000058        25130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRN010000058 DAJTRN010000000
VERSION     DAJTRN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..839)
                     /gene="yeaG"
                     /locus_tag="OO328_002417"
     CDS             complement(<1..839)
                     /gene="yeaG"
                     /locus_tag="OO328_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5930949.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TAT"
     gene            1275..2021
                     /gene="mipA"
                     /locus_tag="OO328_002418"
     CDS             1275..2021
                     /gene="mipA"
                     /locus_tag="OO328_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5930950.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2111..2872
                     /locus_tag="OO328_002419"
     CDS             2111..2872
                     /locus_tag="OO328_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5930951.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(2923..3807)
                     /locus_tag="OO328_002420"
     CDS             complement(2923..3807)
                     /locus_tag="OO328_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5930952.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(3891..4886)
                     /gene="gapA"
                     /locus_tag="OO328_002421"
     CDS             complement(3891..4886)
                     /gene="gapA"
                     /locus_tag="OO328_002421"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5930953.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5228..5641
                     /gene="msrB"
                     /locus_tag="OO328_002422"
     CDS             5228..5641
                     /gene="msrB"
                     /locus_tag="OO328_002422"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5930954.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5683..5955
                     /locus_tag="OO328_002423"
     CDS             5683..5955
                     /locus_tag="OO328_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5930955.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6325..7401
                     /locus_tag="OO328_002424"
     CDS             6325..7401
                     /locus_tag="OO328_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5930956.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7428..8807
                     /locus_tag="OO328_002425"
     CDS             7428..8807
                     /locus_tag="OO328_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5930957.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            8824..9869
                     /locus_tag="OO328_002426"
                     /pseudo
     CDS             8824..9869
                     /locus_tag="OO328_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            9890..10726
                     /locus_tag="OO328_002427"
     CDS             9890..10726
                     /locus_tag="OO328_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5930958.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            10731..11678
                     /locus_tag="OO328_002428"
     CDS             10731..11678
                     /locus_tag="OO328_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5930959.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            11688..12668
                     /gene="ydjG"
                     /locus_tag="OO328_002429"
     CDS             11688..12668
                     /gene="ydjG"
                     /locus_tag="OO328_002429"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5930960.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            12805..13563
                     /locus_tag="OO328_002430"
     CDS             12805..13563
                     /locus_tag="OO328_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5930961.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            13681..15039
                     /locus_tag="OO328_002431"
     CDS             13681..15039
                     /locus_tag="OO328_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5930962.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15132..15773)
                     /gene="pncA"
                     /locus_tag="OO328_002432"
     CDS             complement(15132..15773)
                     /gene="pncA"
                     /locus_tag="OO328_002432"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5930963.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(15784..16800)
                     /gene="ansA"
                     /locus_tag="OO328_002433"
     CDS             complement(15784..16800)
                     /gene="ansA"
                     /locus_tag="OO328_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5930964.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(16967..18823)
                     /gene="sppA"
                     /locus_tag="OO328_002434"
     CDS             complement(16967..18823)
                     /gene="sppA"
                     /locus_tag="OO328_002434"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5930965.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            18984..19535
                     /locus_tag="OO328_002435"
     CDS             18984..19535
                     /locus_tag="OO328_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5930966.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19652..20695
                     /gene="selD"
                     /locus_tag="OO328_002436"
     CDS             19652..20695
                     /gene="selD"
                     /locus_tag="OO328_002436"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5930967.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20700..22661
                     /gene="topB"
                     /locus_tag="OO328_002437"
     CDS             20700..22661
                     /gene="topB"
                     /locus_tag="OO328_002437"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5930968.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            22788..23828
                     /locus_tag="OO328_002438"
     CDS             22788..23828
                     /locus_tag="OO328_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5930969.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23945..>25130)
                     /gene="gdhA"
                     /locus_tag="OO328_002439"
     CDS             complement(23945..>25130)
                     /gene="gdhA"
                     /locus_tag="OO328_002439"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5930970.1"
                     /translation="LVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRF
                     HPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELY
                     RHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGY
                     GLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDE
                     SGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDV
                     DAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQN
                     AARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQG
                     VI"
BASE COUNT         6391 a         5845 c         6487 g         6407 t
ORIGIN      
        1 tgagtggcgg ttaacaaggg atgcagcact tttatcggtg ctttaaacat ctcaacgaat
       61 tccatgatcc cctgattggc gcggcacagc gcaccggaat agccgtaggc gtccgggtca
      121 ttctgcgcgt agtgttcgag tttacgaata tcgactttcc caaccagcgc ggagatgtcc
      181 tggttgttct catcaccggg ttccgttttg gcgatagcaa tttgttgcag aattgacggc
      241 cagaccttca ctacccggaa cttagtgata tcgccaccaa attcatgcag gcgttttgcc
      301 gcccatggcg acatgatggt gccgagataa cggcgaggaa tgccatactc tttttccaga
      361 atctgagcat cttcctgcgg attgaaaaga cagaacggat gatcgttgac cgggctacgc
      421 tcaccgttgg cgctcaatac ataaatcggt acgagctgca ttaatgattt cagtcgctca
      481 gcaagcgatg atttaccccc acccacaggc cccagcagat acaggatttg tttcttctct
      541 tccagcccct gagccgcgtg tttcagataa gagacaatct gttcaatcgc gtcttccatg
      601 ccgtaaaact cttcaaatgc cggataacgt gcaatgaccc ggttagaaaa gagtcgagaa
      661 agtctggggt cctgggctgt atcgaccatg acaggctcac cgatagccat caatagccgc
      721 tcagccgcgt tggcataagc actgcgatct tgccgacaag tggtaagaaa ctcctgcagt
      781 gtgaactctt cgtccttggc agcttcatat cgctggcgat agtgatcgaa tatattcatg
      841 gtatgccgtc ctttcgtttt ttagcacagg ttaagagccg ttcatatgaa tagtagaggc
      901 tcccggaaga ggtaaactga acgacgtctc ttgctaaaac acagcaactc tcatgccatt
      961 ttatcgacga tgagaacaac gtgcccggtg atacaccttc aaaattaagc gtagatggca
     1021 aatggaaaac ttgcctgcac ggctttctta atttcaatgg catatcaaca actcatccat
     1081 aaattttgca taattaatgt aaagaccagg ctcgccagta acgctaaatc catttggctg
     1141 taagcgcggt gtcatccgcg gcaggaaaat taaacagtta ctttaaaaaa tgaaaacgta
     1201 aaaaggttgg gtttcgatgt attgacgggt aaactttgtc gcccgctaaa catttgttta
     1261 aggaatgatt aattgtgacc aaactcaaac ttctggcact tggagtgctt atcgcaacgt
     1321 ctgcaggcgt agcgcacgct gaaggtaaat tttccctggg cgcaggcgta ggtgtcgttg
     1381 agcacccata taaagattac gataccgatg tttacccagt accggtaatc aactatgaag
     1441 gcgataactt ctggttccgt ggcttaggtg gtggttacta cctgtggaat gacgcaacgg
     1501 ataaactttc aattaccgct tactggtcgc cgctttactt caaagcgaaa gacagtggcg
     1561 atcaccaaat gcgtcacctg gatgaccgta agagcaccat gatggctggt ctgtcttatg
     1621 ctcactttac ccagtacggt tacctgcgta ccaccctggc tggcgatacc ctggataaca
     1681 gcaacggcat tgtctgggat atggcctggt tgtatcgtta caccaacggt ggcctgaccg
     1741 tgactccggg tattggtgtg cagtggaaca gcgaaaacca gaacgaatac tattatggcg
     1801 tatcgcgcaa agagtccgct cgcagcggtc tgcgtggcta taacccgaat gacagctgga
     1861 gcccttacct tgagctgagc gccagctaca acttcctcgg cgactggagt gtttacggta
     1921 ccgcgcgcta cacccgtctg tctgatgaag ttactgacag cccgatggtg gataaatcct
     1981 ggactggcct gatttctacc gggatcacct acaaattctg ataatgcatc tttccagggc
     2041 attttgtgca taaaaacagg gcgtcacgcc ctgttttgca ttacggtggt gcaatacctg
     2101 ggaggaaaca atgcaacaaa aaatgattca atttagtggc gatgtctcac tgccagccat
     2161 agggcaggga acatggtata tgggcgaaga tgccagtcag cgcaaaacag aagttgctgc
     2221 actacgcgca ggcattgaac tcggtttaac cctcattgat accgccgaaa tgtatgccga
     2281 tggcggtgcc gaaaaggtgg ttggggaagc attaaccggt ctgcgagaga aggtctttct
     2341 cgtctctaaa gtctatccgt ggaatgctgg cgggcaaaaa gcgataaatg catgcgaagc
     2401 cagtttacgc cgtctcaata ctgactatct cgatctctac ttattacact ggtctggcag
     2461 tttcgctttt gaagagactg ttgctgcgat ggaaaaattg atcgcccagg gaaaaatccg
     2521 tcgctggggc gtttctaacc ttggttatgc cgatatgcag gaactctggc agctgccggg
     2581 gggaaatcag tgtgccacta atcaggtgct ttaccatctc ggttcacgag ggattgagta
     2641 cgatctactc ccctggtgcc agcaacagca gatgccggtg atggcttaca gtccattagc
     2701 ccaggccggg cggttgcgca atggactgtt aaaaaacgcg gtagtcaacg aaattgcaca
     2761 tgtccaacaa catgcagctg cgcttgaggt cgaactttcg tcagcggaat tagctatgct
     2821 ggataaggca tatccggcac caaaaggaaa aactgcgctg gatatggtgt gatgttgata
     2881 cgtgccggat gcatcgcacc cggcacacaa cattaacgca aattaacgtt tggcaacgcg
     2941 aatggattgc gccagatgtg caggtttctc tttggtgact ttttgcgttt ctgaagcgta
     3001 agccgtttct acacaaacaa aggtcttgta gccatcgtcc ggcatgtcgc ccatgctaac
     3061 tgaaagcgcc ggtcccgggt tccagccgac aacgttcaga tgatgctggt ggcctacggc
     3121 gataatacga ttcagcgctt catcattaat cacgctgcaa tcttgcggat tcagatacac
     3181 gcgatcggta cggtcaggga aggtctgaat accgtcggtc agtacatctt ctttcgcgtc
     3241 attcacttta tcaatgaagc gatcgcccag cccactgacg cttaccttag cgatatcacc
     3301 cacgttaaag taggtatgca gggcagaggt ggtttcaaat tcgccatgtg attcaagatc
     3361 gatttcacag gttttaccca cgcggaaatg cgctaacagc gtaaagtcgt gcggccagaa
     3421 ttttttcgtc tcttcgcttt gtgtcaattc aaaagtcagc gctacgccat cagcatcttc
     3481 atgatgtgat ttcagcgtcc acggcaggtt gcgggcaaaa ccgtgcgcag gcagaccttg
     3541 ttgtgccgcc ggaccaaacc acggccagca aaccggtacg ccaccgcgaa tagcgacgcc
     3601 atttttgaac ggtgtgttgt tgctcaacca cagaacttct tcttcacccg caggtttcca
     3661 cgagagaagg tgtgcgccct gtaatgcaaa agaggctttt acctggggat gatcgaccac
     3721 aatgaggtcc agttcatcca gtttacgacg ggagaggaca ggggagattt gttcgatgac
     3781 cggaagggca aaaattttct taatcatgac gcagtccttt aacttcattt tatcaggtaa
     3841 aaaaaagagc gaccgaagtc gctcttttta gatcacagtg tcatctcaac ttatttggag
     3901 atgtgagcga tcaggtccag aactttgttg gagtaaccgg tttcgttgtc gtaccaggat
     3961 accagtttca cgaagttgtc gttcagagcg ataccagctt tagcatcgaa cacggaagtg
     4021 caaacttcgc cgttgaaatc ggtagatact acgtcatctt cggtgtagcc cagaacgcct
     4081 ttcatttcgc cttcagcagc agctttaacg gcagctttga tctgctcgta agttgcagct
     4141 ttttccagac gaacggtcag gtcaactacg gatacgttcg gggtcggaac gcggaacgcc
     4201 ataccagtca gtttgccatt cagttctggc agtactttac ctacagcttt agcagcaccg
     4261 gtagaggacg ggatgatgtt ctgggaagcg ccgcggccgc cgcgccagtc tttgtgagac
     4321 gggccatcaa cggttttctg agtagcggta gtagcgtgaa cggtggtcat cagaccttcg
     4381 atgatgccga agttatcgtt gataacttta gccagcggag ccaggcagtt ggtggtgcag
     4441 gaagcgttgg aaacgatgtc ctggccagca tatttgtcga agttagcgcc tttaacgaac
     4501 atcggagtgt tgtctttaga cggaccagtc ataaccactt tcttcgcacc agcggtgatg
     4561 tgtttacgag cagtttcgtc agtcaggaac agaccagttg cttcagcgac aacgtcaaca
     4621 cccacttcgt cccatttcag gttagccgga tcacgttcag cggtaacacg gattttttta
     4681 ccgttaacga tcagatgacc gtctttcact tcaacggtac cgtcgaaacg gccgtgagtg
     4741 gagtcatatt tcagcatgta tgccatgtaa tcagcgtcta acaggtcgtt gattgcaacg
     4801 atctcgatgt cagaacgttt ctgagcagca cggaaaacaa tgcgaccgat acggccaaaa
     4861 ccgttgatac ctactttgat agtcatatat tccaccagct atttgttagt gaataaaagg
     4921 ttgcctgtaa aattacaaaa accttacgca gcgtcaagcg gaatcgtgtc aatcattgcg
     4981 acaaatcaat cctgtgccta agcattacgc gactgactcg cctcactttt cctttgggct
     5041 tgagaccaca tggggtcggc gccccgaatt ttaaagggca attacgataa aaatgtgatt
     5101 ttcatcacga tttaggtgca gccatttcgt cgagatcaag tttaggacaa aattttgcca
     5161 gttggtgtta atgttttgtt agaatcagtc acgtaatgtg agcacgatta aagtgagatg
     5221 tgagtaaatg gcgaataaac cttcggcaga agaactgaaa aaaaatttgt ccgagatgca
     5281 gttttacgtg acgcagaatc atgggacaga accgccattt acgggtcgtt tactgcataa
     5341 caagcgtgac ggcgtatatc actgtttgat ctgcgatgcc ccgctgtttc attccgaaac
     5401 caagtatgat tccggctgtg gctggcccag tttctacgaa ccggtgagtg aagaatccat
     5461 tcgttatatc aaagacttgt cacatggaat gcagcgcata gaaattcgtt gcggtaactg
     5521 tgatgcccat ctggggcatg tctttcccga cgggccgcaa ccaacaggcg aacgttattg
     5581 tgttaactct gcttctttac gcttcactga tggcgaaaac ggcgaagaaa tcaacggttg
     5641 aaaaaacgat tcagcaactt attccacagg agcggaaaaa ttatgaatct tgatgacatt
     5701 atcaacagca tgacgcctga ggtataccag cgtttgtcga ccgccgttga actggggaaa
     5761 tggcctgatg gcgttgcgtt gaccgaggaa caaaaagaga attgcctgca actggtgatg
     5821 ctgtggcaag cccgccataa taccgaagca cagcatatga caattgacac aaatggtcag
     5881 atggtcatga agagcaaaca gcagttaaaa gaagattttg gtatctcagc aaagcctatt
     5941 gccatgttta agtaagtggt tcatcccctt tttgccgcgg ctatgaaggg gatagccaac
     6001 aatgacccca cgactcgtct caattatctc gacccgacct ggctaagtcc cagtccgatc
     6061 tcttgcagtc gcgttcacgc ctgaatgact tctcctgaac ttaagtctca gacctatttg
     6121 gccggtaatc cctctcgaat ttgctctttt tgcgtcccgc cttccaccaa cctgtaacag
     6181 acccagccat tttctgcgta attgtgattt gagttgtaaa atgcaggtca ttatctgtgt
     6241 tgtgcagatt taatattgac ctcaaaaatc atgtgggtaa gtatgatggc catctaagca
     6301 aaacaaaaca cagaggatga cgcaatgaaa gcactggctc ggtttggcaa ggcctttggc
     6361 ggctacaaga tgattcatgt cccacaaccc atttgtggcc cggaagacgt agtgactgaa
     6421 attaaagccg cggcaatctg cggcgcagac atgaagcact acaatgtcga tagcggttct
     6481 gatgagttta actctatccg cggccatgag ttcgcaggtt gtattgcgca ggttggtgaa
     6541 aaagtcaaag actggaaagt ggggcaacgc gtcgtgtcgg ataacagcgg tcacgtttgc
     6601 ggcgtttgtc cggcctgtga acagggtgat tttctgtgtt gtacagaaaa agtaaacctt
     6661 ggtctggata acaatacctg gggcggtggt ttttccaaat attgtctggt tcctggtgaa
     6721 attctcaaaa ttcatcgtca tgcgttgtgg gaaatccctg atggtgttga ttatgaggac
     6781 gcagccgtac ttgaccctat ctgtaatgcc tataaatcca tcgcgcagca atcgaaattc
     6841 cttcctggtc aggatgttgt cgtcattggc actggcccac tcgggctgtt ttccgtacaa
     6901 atggcgcgga ttatgggggc ggtaaatatc gtcgtcgttg gtctgcaaga agatgtggcg
     6961 gtccgcttcc cggttgcaaa agaactgggg gcgacggcag tagtaaatgg ttctaccgaa
     7021 gatgtggtgg cgcgctgcca gcaaatttgt ggcaaagata atctgggact ggtgattgaa
     7081 tgctccggtg ccaatatcgc actgaaacaa gccatcgaaa tgctccgccc gaatggggaa
     7141 gtggtacgcg ttggaatggg cttcaaacct cttgatttct cgattaatga cattaccgcc
     7201 tggaataaaa gcatcattgg gcatatggcc tatgactcca cctcatggcg taacgctatc
     7261 cggctattag ccagcggcgc tatcaaagtc aaaccgatga tcacgcatcg tatcggcctg
     7321 tcgcaatggc gcgaagggtt tgatgcgatg gtcgataaaa ccgcaatcaa agtgatcatg
     7381 acttacgact ttgatgaata actactctcc tgaaggaacg ttatcgcatg gaacagataa
     7441 caaaaccgca ttgtggtgca cggctggatc gcttaccgga ttgccgctgg cattcgtcaa
     7501 tgtttgccat cgtcgcgttt ggcttgcttg tctgctggag taatgccgtt ggtggcttga
     7561 tcctcgcgca gctgaaagcg ttgggctgga cagataattc cactacagcc acattctcag
     7621 caatcacgac cgccggaatg tttctcggtg cgctggtggg cggcatcatt ggcgacaaaa
     7681 ccggtcgcag aaatgcgttc atcctctatg aggccattca tattgcctcg atggttgtcg
     7741 gtgctttctc accgaatatg gattttctta ttgcctgccg ttttgtgatg gacgttgggc
     7801 tgggagcttt actggttaca ctgtttgctg gtttcaccga atatatgccc ggtagaaatc
     7861 gcggaacgtg gtccagtcgg gtttccttta ttggcaactg gtcatatccg ctctgctcac
     7921 tgattgcgat gggactcacg ccgctgatta gtgcagagtg gaactggcgg gtacaactgc
     7981 ttattcctgc aatattgtcg cttatcgcta cggcgctggc ctggcgctac tttcctgaat
     8041 ccccgcgctg gctggaatcg cgcggacggt atcaggaagc cgagaaagtc atgcggagta
     8101 tagaagaagg cgtcatacgc cagacgggta aacctttgcc gcctgtggtt attgctgatg
     8161 acggtaaagc gccacaagcg gtgccgtatt cagccttact gacaggagta ttactgaaac
     8221 gcgtgatatt aggctcttgt gtgctgatta ccatgaacgt tgtgcagtac acactaatta
     8281 actggttgcc aacaatattc atgacccagg ggattaattt aaaagactcg attgttttaa
     8341 ataccatgag tatgtttggc gcgccatttg gtatttttat tgccatgctg gtgatggata
     8401 aaattccgcg taaaacaatg ggtgtggggc tattaatcct gattgcggtg ctcggatata
     8461 tctattcact gcaaaccagt atgttgctca taacgctgat tggtttcttc ctgattactt
     8521 tcgtctatat gtacgtttgc tatgcctcgg cagtgtatgt ccctgaaatc tggccgacag
     8581 aggccaaact ccgtggctcc ggtctggcga atgcggtagg gcgaatcagt ggtattgccg
     8641 caccttatgc cgttgcagtg ctgctcagta gttatggtgt aacgggagtc tttattcttc
     8701 tgggggctgt ttcaaatatt gtcgcaattg ctatcgccac cattggaatt gaaaccaaag
     8761 gtgtctccgt tgaaagttta agtattgatg cagtagccaa taaataatac gggtggattc
     8821 aaaatgaaaa attcaaaagc aatattgcag gtgccgggca caatgaaaat tatttcagca
     8881 gaaataccag tgcctaaaga agatgaagtt ttgattaaag tagaatatgt cggtatttgt
     8941 ggttcagatg tacatggttt tgaatcaggc ccgtttattc cgcctaaaga cccaaatcaa
     9001 gaaattggcc tgggtcatga atgcgccggg acggttgtgg ctgtgggaag ccgcgtgcgc
     9061 aaatttaaac cgggggatcg ggtaaatatc gaacctggcg ttccttgcgg tcactgtcgt
     9121 tactgtctgg aaggcaaata taacatctgc ccggacgttg attttatggc gacacaaccc
     9181 aactaccgcg gcgcattaac gcactatctg tgtcatccgg agagctttac ttacaaactg
     9241 cccgacaata tggacacgat ggaaggggcg ctggtggagc ctgccgcagt cgggatgcat
     9301 gccgcgatgc tggcagatgt taaaccgggt aagaagataa ttattctggg agcaggttgt
     9361 attggtttga tgacgttgca agcgtgcaaa tgcctgggcg caacggaaat tgccgtcgtt
     9421 gatgtactgg aaaaacgtct ggcaataggg gaacagcctg gtgcgacagt ggttattaat
     9481 ggcgcaaaag aggacactat tgcacgctgt cagcaattta ccgaagacat gggcgcagat
     9541 attgttttcg aaacagcggg ttctgcggtc accgttaaac aggcaccttt aatctggtaa
     9601 tgcgcggcgg taaaattatg attgttggta ctgtacccgg cgattcggca atcaatttcc
     9661 tcaaaatcaa tcgcgaagtc actatccaga cggtattccg ctatgccaat cgttatccgg
     9721 tcacgattga agctatttct tcagggcgat tcgatgtgaa atcgatggtg acgcatattt
     9781 acgattatcg ggatgtacaa caggcatttg aagagtcagt taacaacaaa cgcgacatta
     9841 ttaaaggcgt tattaaaatt agcgattaat taatacggga gaacagaaca tgctcgcaga
     9901 tatcagatat tgggaaaacg atgccacaaa taaatattat gcaattgccc attttaacgt
     9961 atggaatgca gaaatgttga tgggcgttaa agacgcagcc gaagaagcga aatccccggt
    10021 tattatttct tttagtacag gttttgtcgg taacacctca tttgaagatt tctctcacat
    10081 gatggtatca atggcacaaa aagcaacggt gccggtaata actcattggg atcatggtcg
    10141 gagtattgag attattcata acgcctggac tcatggtatg aattcattaa tgcgtgatgc
    10201 ttctgcattt gatttcgaag aaaatattcg tttaaccaaa gaggctgttg atttctttca
    10261 tccgctgggt attccggtag aggcggaatt agggcatgtc ggtaatgaaa ccgtttatga
    10321 ggaagcgctg gcgggctatc actataccga tcctgaccag gcggctgaat ttgttgaacg
    10381 tacgggctgt gactcattgg ctgtcgccat cggcaaccag catggggttt atacgtcaga
    10441 gccgcaattg aactttgagg tcgtcaaacg cgtacgcgat gctgtttctg ttccgctggt
    10501 tttgcatggc gcatcgggga tcagtgatgc cgacattaaa actgcaattt cgttgggtat
    10561 tgcgaaaatc aacattcata cggagctctg tcaggccgca atggtagcag tgaaagaaaa
    10621 ccaggatcag cctttcctgc atttagaacg tgaagtacgt aaagctgtaa aagaacgggc
    10681 attggaaaaa attgaactgt tcggttctga tggcaaagcg gaataatgat atggataatc
    10741 tcgacgttat ttgtataggt gccgctattg ttgatattcc attacaaccg gtcagtaaaa
    10801 atatctttga tgtagattct tatcctcttg aaagaatcgc aatgagcacc gggggcgatg
    10861 caataaatga agcaacaatt atttctcgtc tggggcatcg taccgcatta atgagccgca
    10921 ttggtaaaga tgcctcaggg caatttattc tcgaccattg tcgcaaagag aatattgata
    10981 ttcaaagcct gaaacaggac gtcagcatag atacatctat taacgttgga ctggtgacgg
    11041 aggatggcga gcggacgttt gtcacgaacc gtaacggcag tttgtggaaa ttaaatattg
    11101 acgatgttga ttttgcgcgg tttcctcagg cgaaattatt atcactggcc agtattttca
    11161 acagtcctct attggatggt aaagcgctaa cagaaatttt tacgcaagcg aaagcccggc
    11221 agatgatcat ttgtgccgat atgatcaaac cgcggttgaa tgaaacgctg gatgatattt
    11281 gcgaagcatt gagttatgtc gattatctgt ttccaaattt tgccgaggaa aaattactca
    11341 cagggaaaga gacactggat gaaattgctg actgctttct tgcgtgcggc gtaaaaacgg
    11401 tggtgattaa aacgggtaaa gacggctgct ttatcaagcg tggtgacatg acgatgaagg
    11461 tgccggcggt cgcaggaata accgccatcg acaccattgg cgcgggcgat aactttgctt
    11521 caggttttat tgcggcactg ttagaaggca aaaatctgcg tgaatgcgca cgctttgcca
    11581 atgcaacggc ggctatctcg gttctaagcg tcggtgccac caccggcgta aaaaacagaa
    11641 agctggtcga acaattgctg gaagaatacg aaggataatg aaggcaaatg aaaaagatac
    11701 ctttaggcac gacggatata acgctttcgc gaatggggtt ggggacatgg gccattggcg
    11761 gcggtcctgc atggaatggc gatctcgatc ggcaaatatg tattgatacg attcttgaag
    11821 cccatcgttg tggcattaat ctgattgata ctgcgctagg atataacttt ggcaatagtg
    11881 aagttatcgt cggtcaggcg ttaaaaaaac tgccccgtga acaggttgta gtagaaacca
    11941 aatgcggcat tgtctgggaa cgaaaaggaa gtttattcaa caaagttggc gatcggcagt
    12001 tgtataaaaa cctttccccg gaatctatcc gcgaagaggt agaagccagc ttgcaacgtc
    12061 tgggtattga ttacatcgat atctatatga cgcactggca gtcggtgccg ccatttttta
    12121 cgccgatagc tgaaactgtc gcagtgctta atgagttaaa ggctgaaggg aaaattcgcg
    12181 ctataggcgc tgctaacgtc gatgctgacc atatccgcga gtatctgcaa tatggtgaac
    12241 tggatattat tcaggcgaaa tacagtattc tcgaccgggc aatggaaaac gaactgctgc
    12301 cgctatgtcg tgataatggc attgtggttc aggtttattc cccgctagag cagggattgt
    12361 tgaccggcac tatcactcgt gattacgttc cgggcggcgc tcgggcaaat aaagtctggt
    12421 tccagcgtga aaacatgctg aaagtgattg atatgcttga acagtggcag ccactttgtg
    12481 ctcgttatca gtgcacaatt cccactctgg cactggcgtg gatattaaaa cagagtgatt
    12541 taatctccat tcttagtggg gctactgcac cggaacaggt acgcgaaaat gtcgcggcac
    12601 tgaatatcaa cttatcggat gcagacgcaa cattgatgag ggaaatggca gaggccctgg
    12661 agcgttaaat actcaacacg attcacctct gtttctgtga aaactgtgat gaaaatctgt
    12721 gattcaaata ggttattttg aaagtaaaca tcggttatgc gataatcgcg ctaatgtgat
    12781 gtaaaaagat acaggggtgt aatcgtggcg gcaaaagaca ggattcaggc aattaagcaa
    12841 atggttgcca acgataaaaa ggtgacagtc tcaaatttga gtgggatttt tcaggtaacc
    12901 gaagaaacca ttcgccgcga tcttgagaag ctggaagatg aaggcttttt gaccagaacc
    12961 tatggtggtg ctgttttaaa tacagcggtg ttgacggaga atatccattt ttataagcgc
    13021 gcttcatcgt tttatgaaga gaagctgctt attgcacgca aggcactacc ctttatcgac
    13081 aataaaacca ccatggcagc cgattccagt agtaccgtaa tggaattgct caaattgtta
    13141 caggaccgta gtgacctgac gttgctaacc aactcggcgg aagcgattca tgttctggct
    13201 cagtcagaaa ttaaagtcgt ttcaacgggt ggggaactaa acaaaaatac actttccctg
    13261 caaggaagaa ttactaaaga gatcatcagc cgctaccatg tcgatatcat ggtaatgagt
    13321 tgtaaaggtc ttgatattaa cagcggcgcg ctggactcta atgaagcgga agctgaaatc
    13381 aaaaagacaa tgatccgtca ggcgacagaa gttgcgttat tggttgatca ctctaagttt
    13441 gatcgtaaag cttttgtcca gttagctgac tttagtcata ttaattatat aataactgat
    13501 aaatcaccgg gtgcagagtg gatagcattt tgcaaagaca ataatatcca gctggtgtgg
    13561 taatttaagc acacgttgtc aatattattt gttcctgcga ctggaaataa aatgatgata
    13621 aagatgcggc aagtgaattt gccgcaattg ctacctttta tagcaaatcc gaggcaccca
    13681 atggaacaat atgatcaaat tggcgcaaga ctggaccgct tgcctttggc ccggtttcat
    13741 tatcgtatat ttggtataat aagctttagt ctgttattaa cggggttttt gagttactca
    13801 ggtaatgtcg tcttagcaaa gctggtaagc aatggatggt caaataattt cctctatgcc
    13861 gcctttacct cggcattaat gtttggttat ttcatcggct cacttactgg tgggtttatt
    13921 ggtgactact ttgggcggcg cagggcgttt cgcataaatc ttctcatcgt cggtattgct
    13981 gcaacagggg ccgcttttgt ccctgatatg tactggctca ttttctttcg cttcctgatg
    14041 ggaacaggaa tgggggcgct gattatggtt ggctatgcct catttacgga gtttatcccc
    14101 gcgacggtgc gtggaaaatg gtccgcgcgg ctctcatttg ttggtaactg gtcgcccatg
    14161 ctgtctgcgg cgataggcgt ggtggttatc gcttttttta gttggcgaat aatgtttctt
    14221 ctgggtggta ttggcatact gttagcctgg tttctctcag gtaaatactt tatcgagtcg
    14281 ccacgatggc tggaagggaa agggcaaatc gccggtgcag aaagccaact tcgtgaagta
    14341 gagcagcaaa ttgaaaggga gaagagtatt cgtttacccc agcttacttt gaaccagagc
    14401 aacagcaagg ttaaggtaat caagggtacc ttcaggctcc tgtttaaagg ggaaatgtta
    14461 cgacgtacat tagttgcgat aactgtttta attgcaatga acatttcgct ttataccatt
    14521 accgtatgga taccgaccat atttgttaac tccggcattg atgtcgataa atcaatatta
    14581 atgaccgctg ttattatgat tggtgctccg gtaggaatat ttattgcggc attaattatt
    14641 gatcattttc ctcgtcgatt atttggctcc gccttactta ttattattgc cgtgttaggc
    14701 tatatctatt caagtcagac tacagagtgg gcgattttaa tctatggtct ggtgatgatc
    14761 ttctttttat acatgtatgt ttgcttcgcg tcggcggttt atatcccgga gctttggcca
    14821 acgcatttac gcctgcgcgg ttcgggtttc gttaatgccg tcggacggat cgtcgcagtt
    14881 ttcacgccct atggcgttgc ggcattatta acacattatg ggtcgatcac ggtgtttatg
    14941 gtgcttggtg tcatgttagt gctctgtgcg ctggttctct ccatttttgg catcgaaacg
    15001 cggaaggtgt cgctggaaga gatttctgag gtgaattaaa tctttcgttg atggcatcaa
    15061 ttgccagatg taccactgac gtgttttatt tggcctacaa tgccagtaca gaattgtagg
    15121 ccagcgtaaa attacccctg cgtctcttcc cagtctgcca gcgtatatag cgttgcccca
    15181 gctgctgaca tctccataaa cgcgtgtgca ctgtcctggg gctggatatt cacgccacga
    15241 cagccatcgg taatcacgtt taccttataa cctaactgta acgcgtccag cacggtaaac
    15301 ttcacgcaat agtcagtagc caggcccata acgatcaatt catcgatttc atgatcgcgc
    15361 aaccagtcat caagcgcggt tttctgccga cgtccgttat caaaaaaggc actgtaactg
    15421 tcaactaaag gattttcgcc tttatggaac accgctgcga tctctttttg attcagtaac
    15481 ggatgtaatt gtgcgccttc actgttctgc acacagtgtt caggccagaa agtttgccgc
    15541 aggccgccga gttggcctgg cgtataaggc tctacgccgt gctggctggc aaagctgccg
    15601 tgattcgccg gatgccagtc ctggctggcg ataaccgctt ctccgcgcga ctggcaccag
    15661 tcaataaggc ggttagcaac ctccaccgta ctgtcacctt ccggtacggc gagcgcgcca
    15721 ccagcacaga aatcattttg taaatcgacc agtaacaggg cgcgatgggg cattgcagtc
    15781 tccttaatca tccggcgtca gttcgccgcg caggttttgg ctcatggcct tgcgaatggt
    15841 ttcagtatcc agttcctggc tcagcaggta atgcagtttg gttagcgtgg cttctacagt
    15901 catatctgca ccgtcaataa cgccggcatg ggcgagggcg ttaccggtgg cataaccact
    15961 catattcact ttaccggaca tacattgtgt caggttgacc accacaatac cgcgatcgct
    16021 ggcttcttgt aattcctgca ggaaggcttt gttttgtggc gcattaccca cgccatagga
    16081 gcgcagaatc aatgctttca ccggttggcg cagaaaattg cgcacgacgt cagcagaaat
    16141 ccctggataa atcgtcacca cgccaattgg ttgtggcgtg attggatgaa cgattaattc
    16201 cccttcaccg tgcggggcgg gtggcgtatt caaacgacga atatggatac cagcttccag
    16261 taacggagga aggtttggag aggcaaacgc atcaaaacca tcggcatggg ctttggtagt
    16321 gcggttgccg cgatacaatc ggttattgaa aaagagcgtt acttcgttga tcggataatt
    16381 cggcgcaacg tacaacgcat tcagcagatt aatttgtccg tcagagcgta actcagccag
    16441 cgggatttgt gaccctgtca caatgaccgg tttaccgaga ttctcgagca tgaacgacag
    16501 cgcagaggcg gtatacgcca tcgtgtcggt accgtgtaga atgacaaaac catcatagtc
    16561 gtcatagtgc gctttaatat cttcagcaat atgctgccag tcttccggcg tcatatcaga
    16621 agaatccatc aacggcgtat attcatgaat ggtgaaatct ggcatctccg ggcgatggaa
    16681 ttccggcatc agcgccagtt ggcgttgtag atgacctgac accggaatat aaccctgctc
    16741 ggaacgctgc atcccgatgg tcccgcccgt gtaggcgacg taaattgatt tcttttgcat
    16801 gatattgata tgaatgatat cgaaagagca aaagtatata cgtgaggcag gaggagcgca
    16861 cctgatacta tgcggataag gaaatatggg gcgagacagc gggaagagga ttcgtcgagg
    16921 taaaaaataa tgtggccgac gatctgtcgg ccactcagta caagacttaa cgcacgttgg
    16981 cgcaggtcag gcaaaacgca taacggtttt gtgggtcgtt aaacgcggcc agcttgtcgc
    17041 tttcactttt aacggtagag gctaccgagg caagcggtgc aggtaacatg gcctggaacg
    17101 catctggcaa cattgcccgg acagaaccag acatgttgtc catcactttg tcgaagaagg
    17161 tcggttcatc aacgtagtat tccagatgcc actgtttcac ttttgcgagc tctgctgctt
    17221 tggcaaccgc atcatcgaaa tccccgagac tatcgaccag cccgttagct tttgcatcct
    17281 gaccggtcca gacgtggccc tgggcgattt tatcaatttg ctccggcgtc gaatgacgcg
    17341 catcagcaac cagcgtgata aagcgtttat agccattctc aatgcttaat tgcatcatct
    17401 gctgcgcttc cggcggcagt gccctggtga tagaaacatc cgccagcggt gaagttgaga
    17461 caccatcagt atgaacacca atcgaatcca gactattttc tacggtggtg atcacgccga
    17521 agataccgat agaaccggtc agggtgctgg ggttagccac aatgtaatta gctggcgtgg
    17581 aaatccagta accaccagat gccgccatgc cgcccatcga tacaaccaca gacttacccg
    17641 ctgcccgggc tgctgccagt tcagcgcgaa tcacttcaga cgcggtaacg ctgccgcctg
    17701 ggctattaac acgcaggaca atcgctttca ctttcgggtc aaggcgagcg tcgcggattt
    17761 gtgccgcagt ggtatcaccg ccaacattcc cctgagtttc ctcgccatcc ataattgcgc
    17821 cattagcgaa gacgacaccg atgctgtcac cggtatctgc cggcgttttc aatgcgtaat
    17881 cgtaataact gatggcgcga taatttttat cagtcttact ccagccgaac tctttggtca
    17941 gagctttttc gatttcggca ctcgatgcca gtgcatcgac cagcttgttt tccagtgcat
    18001 atttcgcggt atcgccaccg gttttggtta aaccctcaag caacccttgc gcgccaggga
    18061 atacctgctg agcagggatc tgccggttag cggcaacagt attcagatag ttttgccaca
    18121 gctcaccaat ccagcggctg tcagcttcgc gggctgccgg tgacatatca tcacgaataa
    18181 acggttcaac ggcagattta tacgtaccca cgcggaacac atgggtggaa actttcagct
    18241 tatccagcaa cgatttgtag tacagaccgt tggtagcaaa accgtgcaga tcaaccacgc
    18301 cttgcggtga cagccaaatt ttattggcga aactggcgag ataatattgc ccctggctgt
    18361 agttctcgcc aacggcataa accggtttcc cgctgtcacg aaactctttc agggctttgc
    18421 cgatgtactg catagacggc tggtcgccgc ctgcgaagtt tttcagatcc atcacgatac
    18481 cggtgatatt gcggtcgtcc ttcgcctggc gaatggtgtt gacgatatca aacagtgagt
    18541 tttcctgcag acgatcggaa ctggcaccaa gcagctggcg gcttaattta ctaaaccgct
    18601 gagaactgtc tggtttatcg acgatcacac cagaaatgtc cagcagcagt gcgccacgac
    18661 tggccgtttc tttcgaatca ccaccactga cctgcatcca gatccccaca ccaaccagta
    18721 cgaggaaaat aaagaacagg ttaagtacca tttcacggac gaaattcagc agacgccacg
    18781 tccatttaaa aaatccggca ataaatcgcc aaagggttcg catgtattct cccaacttaa
    18841 ggtcacacct gtcacaggcg caatagatgt ggttatcgta atgacccaac cggcacttgt
    18901 cagcaggaat tgcggactgt gctgtaacaa aatccactct cgtgttaatt ttgtgaataa
    18961 atatcacgac aggagttaat caaatggatg cactcgaact attgatcaat cgccgtagcg
    19021 cctcccgcct ggctgaaccc gcgccaacgg gtgaacaact gcaaaacatc ctgcgtgcgg
    19081 gtatgcgtgc gccggaccat aagtccatgc aaccgtggca tttttttgtg attgaagggg
    19141 aagggtgcga gcgtttcagc gccgtactgg aacagggggc gattgctgcc ggtagtgatg
    19201 ataaagctat cgacaaagcc cgtaatgcgc cgttccgcgc accgctcatc atcacggtgg
    19261 tggcgaaatg caaagaaaat cataaagtcc cgcgatggga acaggaaatg tctgccggat
    19321 gcgcggtcat ggcgatgcaa atggcagccg ttgcccaggg atttggcggc atctggcgca
    19381 gtggcgcatt aactgaaagt ccggtagtgc gtgaagcatt cggttgccgt gagcaggata
    19441 aaattgtcgg ttttctctac ctcggcacgc cgcagttgaa agcatctacg tcgattaacg
    19501 tcccggaccc gacgccgttt gtaacttatt tctgataatt gtcgcgaaac tgtctggatt
    19561 gtgagcaaag gcgcaggaat tcagacactc tcacttatca cttcacggaa tgagggttac
    19621 catacccgga ttgcattgac aggagatgtc catgagcgag aactcgattc gtttgaccca
    19681 atacagccac ggagctggtt gcggctgtaa aatttcccca aaagtgttgg aaaccatcct
    19741 gcacagtgag caggcgaagt ttgttgatcc gaatttgctt gtgggtaatg aaacccgcga
    19801 cgatgcggcg gtgtacgatc tgggcaatgg caccagcgtt atcagtacca ccgacttctt
    19861 tatgccgatc gttgataatc ctttcgattt tggccgcatt gcggcgacta acgccatcag
    19921 cgatatcttc gcgatggggg gcaaaccgat tatggcgatt gcgatcctcg gctggccgat
    19981 taacaaactt tccccggaaa ttgcccgcga agtaaccgaa ggtggacgct atgcatgccg
    20041 tcaggcgggt attgcgctgg ctggcggtca ctccatcgat gcgccggagc cgatttttgg
    20101 tctggcggta acggggatcg taccgaccga gcgggtgaag aaaaacagta ccgcacaagc
    20161 cggatgcaaa ctgttcctga cgaaaccgct ggggatcggc gttcttacca cggctgagaa
    20221 aaaatcactg ttgaaaccag aacatcaggg actggcgacg gaagtgatgt gccggatgaa
    20281 catcgcaggc gcgtcctttg ccaacatcga aggcgtaaaa gcgatgaccg acgttacggg
    20341 ctttggtctg ctgggccact tgagcgaaat gtgtcagggg gctggtgtgc aggcacgcgt
    20401 cgactatgaa gcgatcccga aactccccgg tgttgaagag tacattaagt tgggcgcagt
    20461 acctggcggc actgaacgta actttgccag ctacggtcat ctgatgggtg aaatgccgcg
    20521 tgaagtgcgc gatctgctgt gcgatccgca aacttctggc ggtttgctgc tggcggtcat
    20581 gccggaagca gaaaatgagg ttaaaactac agccgccgag tttggcattg aactgacggc
    20641 gattggcgaa ctggtgccag cgcgcggcgg tcgtgccatg gttgagatcc gttaatttaa
    20701 tgcggttgtt tattgccgaa aaaccgagtc tggcgcgcgc cattgctgat gtcctgccca
    20761 aaccgcaccg gaaaggcgat ggctttatcg agtgcggtaa tggtcaggtg gtgacttggt
    20821 gtatcggtca cctgcttgag caggcgcagc cagacgccta cgacagccgc tatgcgcgct
    20881 ggaatcttgc ggatttgccg attgtcccgg aaaagtggca attacagcca cgaccctccg
    20941 tgaccaaaca acttaacgtc atcaaacgct tcctgcatga agccagcgaa atcgttcacg
    21001 ccggggaccc ggatcgtgaa gggcaattgc tggtggatga agtgctggac tatctgcaac
    21061 tggcaccgga aaagcgccag caggtgcagc gttgcttgat aaacgacctg aacccgcagg
    21121 cggttgagcg ggcgatcgac cgtcttcgtt ccaacagtga gtttgtgcca ttgtgcgttt
    21181 ctgcgctggc gcgagcgcgt gccgactggc tgtacggcat caatatgacc cgtgcgtata
    21241 ccattctcgg tcgcaatgcc ggttatcagg gcgtactttc cgtgggacgc gtgcagacac
    21301 ccgtgctcgg gctggtggtg cgccgcgatg aagagattga aaacttcgtg gcgaaagact
    21361 tctttgaagt caaagcacat atcgtgacac ctgccgatga gcggtttacc gctatctggc
    21421 aaccgagcga agcgtgtgaa ccgtaccagg atgaagaagg gcgcttgtta catcgtccac
    21481 tggcggagca tgtggttaac cgcattagtg gtcaaccggc tattgtcacc agctataacg
    21541 ataaacggga atcagaatcc gcgccgctgc cgttttcgct ttcggcgttg cagattgaag
    21601 cggcaaaacg cttcggtctg agcgcgcaga acgtgcttga tatctgccag aagctgtacg
    21661 aaacgcacaa gctaatcact tatccgcgtt ccgattgtcg ctatttgcca gaagaacatt
    21721 ttgccggacg ccacgcggtg atgaatgcca tcagcgttca tgcaccggat ctgttgccgc
    21781 agccagtggt agatccagat atacgcaacc gctgttggga tgacaaaaag gtcgatgcgc
    21841 accacgccat catcccgacc gcacggagtt ctgcgatcaa cctgacggag aacgaagcga
    21901 aggtctataa cctgattgcc cgtcaatacc tgatgcagtt ctgcccggat gcggtgttcc
    21961 gcaagtgtgt tatcgaactg gacattgcca aaggcaaatt tgtcgctaaa gcccgttttc
    22021 ttgctgaagc aggctggcgc acgctgttag gcagcaaaga gcgcgatgaa gaaaacgacg
    22081 gcacgccatt gcctgtggtg gcgaaaggcg atgagttgct gtgtgaaaaa ggtgaagtgg
    22141 tagagcggca aacccagccg ccgcgccatt ttaccgatgc aacactgctt tcggcgatga
    22201 ccgggatcgc gcgctttgtg caggacaaag atctgaaaaa ggtccttcgt gcgaccgatg
    22261 gtctggggac agaagcaacc cgtgccggga ttattgaact gctgttcaag cgtggttttc
    22321 tgaccaaaaa agggcgctat atccactcca ccgacgccgg aaaagcgtta ttccattcgc
    22381 tgccagaaat ggcgacgcga ccggacatga ccgcgcactg ggaatcggtg ctgacgcaaa
    22441 tcagcgaaaa gcagtgtcgc tatcaggact ttatgcagcc gctggtgggg acgctatatc
    22501 agcttattga tcaagccaaa cgtacgccgg tgcggcagtt tcgcggcatt gtggctccgg
    22561 gcagtggtgg cagtgctgat aagaaaaagg ctgcaccgcg taaacgtagt gcgaaaaaaa
    22621 gtccgccagc agatgaagcc ggaagcgggg cgatagcgta agcgagcgaa tctttcgtgc
    22681 tattcgagtc atattctgaa atatccagcg gatcaagaaa gttcgttgga tatttttttg
    22741 catggataaa attatcgcct ctaaagtatg taataacagg gaatgtgatg aaaaaagttt
    22801 tattgcaaaa ccatcctggg agcgagaagt attcttttaa tggctgggga atatttaata
    22861 gtaattttga acgggtgatt aaagaaaata aggccatgct gctttgtaag tgggggtttt
    22921 atttaacatg tgttgtcgct gtaatgtttg tattcgcagc gataacatcc aacggtttga
    22981 atgaaagagg cctgattacc gcgggatgct cttttcttta tctattaatt atgatggggc
    23041 ttattgttcg ggccggtttt aaagcaaaaa aagaacaact gcattattat caggctaaag
    23101 gtattgagcc gctcagtatc gagaagttac aggcgctaca attgatcgca ccttatcgat
    23161 tctatcataa gcaatggtct gaaacgctag agttctggcc gcgaaagcct gaacctggca
    23221 aagatacctt ccaatatcat gtgcttcctt ttgactcgat cgatatcatc agtaaaagac
    23281 gcgagtcttt agagaatcaa tggggtatcg aagatagcga aagttattgt gccttaatgg
    23341 agcattttct ttctggcgac catggagcca atacctttaa agcaaacatg gaggaagccc
    23401 cagagcaggt tatcgccttg ttgaataaat ttgctgtttt tccctcagac tatatctctg
    23461 attgcgctaa taatcgctcc ggtaaatcct cggcgaagct aatatgggcg gcggaattat
    23521 catggatgat ctcgatatca agcacagctt ttcaaaacgg gacaattgaa gaagaactgg
    23581 cctggcatta tataatgctt gcttctcgaa aggcgcacga gttgttcgaa agcgaagaag
    23641 attatcaaaa aaatagtcta atgggatttc tttactggca tatctgctgc tatcgcagaa
    23701 agttaacgga tgctgaactt gaagcgtgtt atcgttacga caagcagttt tgggagcact
    23761 acagtaagaa atgccgttgg cccataagaa atgttccctg gggagcatca tccgttaaat
    23821 actcataaac gcctgaaatt ttgccggggg cgctgcgctt gaccggccta cattagagcg
    23881 taactgcaat gaattgtgcc catctgtagg cctgataagc gtagcgccat caggcattta
    23941 caacttaaat cacaccctgc gccagcatcg catcggcaac cttcacaaaa ccggcaatgt
    24001 tcgcgccctg cacgtagttg gtttgctcac cttcaccacc atgctcaaca caggcatggt
    24061 ggatatccag catgatgtga tgcaaacgtg cgtcaacttt ctcggctttc cagcccaggc
    24121 gcgcagcgtt ttgtgccatt tccaggcccg acgttgctac accgccagca ttagccgctt
    24181 tacccggtgc aaacagtacg cctgcctgct ggaacagttc agtcgcttcg atggtggtcg
    24241 gcatatatgc cccttcagcg acggctttaa cgccattggc gataagctga tgcgcggcgt
    24301 caacatccag ttcgttctgg gtggcgcaag gcagggcgat atcaaccggt acagaccacg
    24361 gctgttggcc ttcgagatag accagaccaa attctttggc gtaatctgcc gctcgaccat
    24421 cgcggctggc tttgatttcg ataagacgtg ccagtttctc tttcgtgaat ccgctttcat
    24481 caactacagt gccgctggag tctgacgcag tgatcacacg agcaccaaat tccatcgctt
    24541 tttcgatagc gtactgggcg acgttgccgg agccagaaac ggaaacgctc atcccttcaa
    24601 aacccatacc gtggcgtttt aacattgctt ctgtgaaata aaccagaccg tagccggtag
    24661 cttccgggcg aataagacta ccgccaaatg aaaggccctt accggtgaag acgcaggcgg
    24721 tattgttgga gagctttttc atcatccccg ccataaagcc gacttcacga ccaccaacgc
    24781 cgatatcacc tgccggaacg tcggtatccg cgcccaggtg gcgatacagt tcagtcatca
    24841 gcgtctggca aaaacgcatc actttacctt cgctttttcc tttcggatcg aaatcgctgc
    24901 cgcctttacc accgcccatc ggcagggtag tcagggcatt tttgaaggtt tgttcaaagc
    24961 cgaggaattt gagaatggaa aggttaactg acggatggaa gcgcataccg cctttgtacg
    25021 ggccgatggc agagctgaac tgcacacgcc atgcacggtt gacctgtatc tggttgcgat
    25081 catcaaccca taccacgcga aactggatca cgcgctccgg ttcaaccaga
//
LOCUS       DAJTRN010000059        24341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRN010000059 DAJTRN010000000
VERSION     DAJTRN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            598..2526
                     /gene="thrS"
                     /locus_tag="OO328_002440"
     CDS             598..2526
                     /gene="thrS"
                     /locus_tag="OO328_002440"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5930971.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2530..3072
                     /gene="infC"
                     /locus_tag="OO328_002441"
     CDS             2530..3072
                     /gene="infC"
                     /locus_tag="OO328_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5930972.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3169..3366
                     /gene="rpmI"
                     /locus_tag="OO328_002442"
     CDS             3169..3366
                     /gene="rpmI"
                     /locus_tag="OO328_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5930973.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3419..3775
                     /gene="rplT"
                     /locus_tag="OO328_002443"
     CDS             3419..3775
                     /gene="rplT"
                     /locus_tag="OO328_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5930974.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3892..4016
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3898..3942
                     /gene="pheM"
                     /locus_tag="OO328_002444"
     CDS             3898..3942
                     /gene="pheM"
                     /locus_tag="OO328_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5930975.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4225..5208
                     /gene="pheS"
                     /locus_tag="OO328_002445"
     CDS             4225..5208
                     /gene="pheS"
                     /locus_tag="OO328_002445"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5930976.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5223..7610
                     /gene="pheT"
                     /locus_tag="OO328_002446"
     CDS             5223..7610
                     /gene="pheT"
                     /locus_tag="OO328_002446"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5930977.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7615..7914
                     /gene="ihfA"
                     /locus_tag="OO328_002447"
     CDS             7615..7914
                     /gene="ihfA"
                     /locus_tag="OO328_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5930978.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8015..8995
                     /gene="btuC"
                     /locus_tag="OO328_002448"
     CDS             8015..8995
                     /gene="btuC"
                     /locus_tag="OO328_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5930979.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9058..9609
                     /gene="btuE"
                     /locus_tag="OO328_002449"
     CDS             9058..9609
                     /gene="btuE"
                     /locus_tag="OO328_002449"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5930980.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9609..10358
                     /gene="btuD"
                     /locus_tag="OO328_002450"
     CDS             9609..10358
                     /gene="btuD"
                     /locus_tag="OO328_002450"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5930981.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10436..10900
                     /locus_tag="OO328_002451"
     CDS             10436..10900
                     /locus_tag="OO328_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5930982.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11147..11860
                     /gene="ydiV"
                     /locus_tag="OO328_002452"
     CDS             11147..11860
                     /gene="ydiV"
                     /locus_tag="OO328_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5930983.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11924..13360
                     /locus_tag="OO328_002453"
     CDS             11924..13360
                     /locus_tag="OO328_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5930984.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13364..13555)
                     /gene="hemP"
                     /locus_tag="OO328_002454"
     CDS             complement(13364..13555)
                     /gene="hemP"
                     /locus_tag="OO328_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5930985.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13687..14733)
                     /gene="aroH"
                     /locus_tag="OO328_002455"
     CDS             complement(13687..14733)
                     /gene="aroH"
                     /locus_tag="OO328_002455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5930986.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14890..15723)
                     /gene="ppsR"
                     /locus_tag="OO328_002456"
     CDS             complement(14890..15723)
                     /gene="ppsR"
                     /locus_tag="OO328_002456"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5930987.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16055..18433
                     /gene="ppsA"
                     /locus_tag="OO328_002457"
     CDS             16055..18433
                     /gene="ppsA"
                     /locus_tag="OO328_002457"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5930988.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18490..20136)
                     /gene="fadK"
                     /locus_tag="OO328_002458"
     CDS             complement(18490..20136)
                     /gene="fadK"
                     /locus_tag="OO328_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5930989.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20193..20486)
                     /locus_tag="OO328_002459"
     CDS             complement(20193..20486)
                     /locus_tag="OO328_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5930990.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20483..21772)
                     /locus_tag="OO328_002460"
     CDS             complement(20483..21772)
                     /locus_tag="OO328_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5930991.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21828..22766)
                     /locus_tag="OO328_002461"
     CDS             complement(21828..22766)
                     /locus_tag="OO328_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5930992.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22786..23550)
                     /locus_tag="OO328_002462"
     CDS             complement(22786..23550)
                     /locus_tag="OO328_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5930993.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23869..>24341
                     /locus_tag="OO328_002463"
     CDS             23869..>24341
                     /locus_tag="OO328_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5930994.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6010 a         5911 c         6408 g         6012 t
ORIGIN      
        1 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
       61 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      121 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      181 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      241 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      301 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      361 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      421 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      481 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      541 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      601 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      661 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
      721 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
      781 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
      841 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
      901 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
      961 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1021 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1081 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1141 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1201 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1261 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1321 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1381 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1441 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1501 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1561 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1621 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1681 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     1741 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     1801 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     1861 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     1921 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     1981 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2041 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2101 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2161 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2221 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2281 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2341 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2401 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2461 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2521 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2581 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2641 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     2701 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     2761 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     2821 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     2881 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     2941 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagta
     3001 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3061 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3121 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3181 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3241 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3301 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3361 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3421 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3481 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3541 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3601 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3661 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     3721 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     3781 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     3841 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     3901 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     3961 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4021 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4081 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4141 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4201 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4261 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4321 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4381 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4441 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4501 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4561 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4621 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4681 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     4741 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     4801 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     4861 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     4921 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     4981 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5041 gggatggtgc atccgaacgt gctgcgtaac gttagcatcg acccggaagt ttactctggt
     5101 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5161 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5221 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatc gatagcgata
     5281 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5341 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5401 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5461 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5521 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5581 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5641 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     5701 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     5761 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgactatcg
     5821 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     5881 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     5941 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6001 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6061 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6121 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6181 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6241 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6301 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6361 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6421 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6481 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6541 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6601 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6661 aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     6721 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     6781 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     6841 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     6901 agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     6961 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7021 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7081 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7141 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7201 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7261 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7321 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7381 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7441 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7501 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7561 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7621 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7681 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     7741 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     7801 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     7861 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     7921 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     7981 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8041 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8101 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8161 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8221 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8281 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8341 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8401 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8461 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8521 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8581 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8641 gccgatgaat atgttagcgc ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     8701 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     8761 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     8821 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     8881 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     8941 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9001 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9061 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9121 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9181 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9241 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9301 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9361 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccac agtcgcgccg
     9421 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9481 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9541 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9601 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9661 cgctttcagg cgaggttcgg gctggggaaa tcctgcacct ggtggggccg aatggcgcgg
     9721 gtaagagtac cttactggcg cgaatggccg gaatgaccag cggtaaggga agcattcagt
     9781 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
     9841 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
     9901 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
     9961 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10021 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10081 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10141 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10201 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10261 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10321 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10381 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10441 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10501 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10561 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg
    10621 ttcgggattt gtggttgtga cgatgcgcga tcgtttcgat ttgcatctgc cccgtgaaac
    10681 caaacaacaa gcctctattg gcacgcaaat tgataaagac gagttgctgc ctggtgacct
    10741 ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa
    10801 caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt
    10861 ctattggcag aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa aattgacggc
    10921 gatgatacat cgccgtcaat ttactcctga atacagtagt attttgcaga caaaaattcc
    10981 gtaaatggat tattcgttta taatcaggat aatctaaaaa aaggccccct gaaaaagtgg
    11041 ttaacatgga acaaatgaaa gttactcctt ctgtaattgt tatgtcttat ggcctcatcc
    11101 cttttagccg gatactgaaa aacatccttc gagagggacg gttaccatga agattttttt
    11161 ggagaatctt tatcattctg attgctattt cctgccgata agagataacc agcaactcct
    11221 tgtcggcgtt gagttaatta cccatttctc cagtgaagat ggcacggttc gtatcccgac
    11281 gagtcgggtt atagcgcaac ttaccgagga gcaacattgg caattatttt cagagcagtt
    11341 ggaattacta aaatcatgcc aacacttttt tattcaacat aaactctttg cctggcttaa
    11401 tttaacgcca caagtggcta cattattatt agacagggat aattacgcag gcgaactatt
    11461 aaaatatccg tttattgagc tgttgattaa tgagaattac ccgcatctca atgaaggaaa
    11521 agataatcgc gatcttttgt ccttatcgca aatgtatccc cttgtgttag gcaatttggg
    11581 agcaggtaat agcacaatga aagcggtttt tgatggtctt ttcacccgcg taatgctgga
    11641 gaaaggtttt attcagcaac aaatcacgca tcgctccttc gaaccgttta ttcgtgcgat
    11701 ccaggcgcaa atttcccctt gttgcaattg catcatcgcg gggggcattg atacgccaga
    11761 aatattggca cagattaccc ccttcgattt ccatgctctc cagggatgcc tgtggcctgc
    11821 cgttcctata aatcagataa cgacgttggt tcagcgataa ccctcctgtt tgctggtgtt
    11881 taagacgaga gtaaccgtct acactatcaa acagatagga tctatgaccc tgtcttttat
    11941 tacccgctgg cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa
    12001 taatgcccgg ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc
    12061 gctgtttaaa aatgccgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc
    12121 actggcacag gtttacagtg gtcatcagtt cgtcgtctgg gcgggccaac tgggtgatgg
    12181 gcgcggcatt ttacttggcg aacaactgct tgctgatggc actacaatgg actggcatct
    12241 gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc
    12301 gacgatacga gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg
    12361 cgcgttaagt atcgtcacca gcgattcgcc agtgtatcgg gaaacggtgg agccaggcgc
    12421 gatgctgatg cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta
    12481 tcgccgcgag ccggaaaagg ttcgtcagtt ggctgacttt gccattcgtc attactggtc
    12541 acatcttgaa gacgatgagg acaaataccg tctctggttt aacgatgttg tcgcacgtac
    12601 cgcgtcgtta attgcccaat ggcagacggt cggctttgca catggggtga tgaataccga
    12661 caacatgtcg ctgctgggac tgacgcttga ttacgggccg tttggttttc ttgatgatta
    12721 cgaacccggt tttatttgta atcactcgga tcatcaaggg cgttatagct ttgataatca
    12781 acctgctgtc gcgttgtgga atttacagcg tctggcgcag acattgtcac catttgttgc
    12841 cgtagatgcc ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg
    12901 acaacggatg cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact
    12961 gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat
    13021 gctgagtctg accgagcagc acagcgcggc gtcaccgcta cgtgaggagt ttattgatcg
    13081 tgcggcattt gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag
    13141 cgatagtgag cgtcagcaac tgatgcaaag cgttaaccca gctctggtat tgcgcaactg
    13201 gttggcgcaa cgggcgattg aagcggcaga aaagggtgat atgatggaat tgcaccgcct
    13261 gcatgaggcg ttgcgaaatc ctttcagcga cagagatgat gactatgtca gtcgtccacc
    13321 tgactggggt aaacggctgg aagtcagctg ctcgagttaa aggctactta gttaacagca
    13381 gcttgccagc ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt
    13441 tgccatccgg acctaacagt gtctggctgg taattcgccg gatgggctgc ggggaagcgg
    13501 tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa
    13561 ttaataataa ttgatataac aatgattcct attatcaata ttatggcaga ccagagcaag
    13621 cagtaatgtg ttgagaagac aacaagttgc gcagaaatgc ccatcccatc aggaatgggc
    13681 acgcattcag aagcgggtat ctaccgcaaa ggcgagtttt tcgaccaggc gttcggtatc
    13741 ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gaccgccgac
    13801 gattttttgc gttccttcgc gcaggaaact ttccgccata attccagcaa tctccgtaga
    13861 gccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt
    13921 ctggcagtta ccgtggctga aatccaccac cagatgttca ggtaaatcaa actcgtgcag
    13981 cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat
    14041 aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac catttttgtc
    14101 tggcgagagg aacatatggc tggcgcgggc tgcgcggata gcatccacgg caatccgcgt
    14161 gttgccatcg gtaccatttt taaaacctac cggacaggag agtgctgaag ccatttcgcg
    14221 gtggatctga ctttcggtag tacgagcgcc aatcgcgccc caactgatta aatcagcaat
    14281 aaactgaccg gttaccatat cgaggaactc agtcgcggta gggacaccca gctcatttac
    14341 ctgtaaaagt aatttgcgcg ccagctccag accgtgattt acccgatagc tgccgtttaa
    14401 atccggatcg gagattagcc ctttccagcc gacaacagtt cgtggttttt caaaataggt
    14461 gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc
    14521 gtactccatt gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg
    14581 cttatcttca ccattcagta ttttttcaat tctgcggcgg gagtcggtga catgggtggc
    14641 gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat
    14701 acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct
    14761 acggcaggac taccggaaag tgatgcgcat cacgataaac caatcgcagc tgatttcaag
    14821 tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaac aaaccaatgc
    14881 actagttctc tagtacattc ggcgactaag gcccatgata tcgaggatct tggtggcaat
    14941 ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaaggc
    15001 ttccacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcgac tgttctcccg
    15061 acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttatg
    15121 ctgaagcggt ttgagcgacg cgggtagcac cagattatcc atatcgtcgg caataaaggg
    15181 gtagtttgcc gcgcggatac caaattgcat tgccagatac agactggtgg gggttttacc
    15241 acagcgagaa acaccgagca ggatcacctg agcctgatcc agattgcgca acgaaatgcc
    15301 gtcatcgtgg gcgagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt
    15361 attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg
    15421 ttgtagcggg gcaaccagcg cctgaacgat atcctggcaa aagccttcac tttgcaagat
    15481 gatggcgcga atctccggca acacgatgga gtagaagacc agcgggcgca cgcctgtctg
    15541 gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa
    15601 cggcagcgtg atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac
    15661 ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc
    15721 cattttgttc ttcccgtgaa gcagacataa ttctatgaaa gcataaatta aaagcgcaca
    15781 gaagcgtagg acgtaatgtc tggtttataa aatgaacctt cattttattt tttataaaaa
    15841 cagcatttca tttttatggt ttcgtttata ccgatggttt atgtggaaat tgtcgaatag
    15901 agcaggtttg cgcaacgctg ggatcagtct taaaaagtaa aaaaatatat ttgcttgaac
    15961 gattcaccgt ttttttcatc cggttaaata tgcaaagata aatgcgcaga aatgtgtttc
    16021 tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc aacaatggct cgtcaccgct
    16081 ggtgctttgg tataaccaac tcggcatgaa tgatgtagac agggttgggg gcaaaaatgc
    16141 ctccctgggt gaaatgatta ctaatctttc cggaatgggt gtttccgttc cgaatggttt
    16201 cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa accagcgcat
    16261 ttatgaactg ctggataaaa cggatattga cgatgttact cagcttgcga aagcgggcgc
    16321 gcaaatccgc cagtggatta tcgacactcc cttccagcct gagctggaaa acgccatacg
    16381 cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc tcttttgcgg tacgctcctc
    16441 cgccaccgca gaagacatgc cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa
    16501 cgttcagggt tttgacgccg ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa
    16561 cgatcgcgcc atctcttatc gtgtgcacca gggttacgat caccgtggcg tggcgctctc
    16621 cgccggtgtt cagcggatgg tgcgctccga cctcgcatct tctggcgtga tgttctccat
    16681 tgataccgaa tccggctttg accaggtggt gtttatcact tccgcatggg gccttgggga
    16741 aatggtcgtg cagggtgcgg ttaacccgga tgagttttac gtgcataaac cgacactggc
    16801 ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc gcatggttta
    16861 cgcgccgacc caggagcacg gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg
    16921 tgacatcttc tcgctgacca acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat
    16981 tgagaaacac tacggtcgcc caatggatat tgagtgggcg aaagatggcc acaccggcaa
    17041 actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg
    17101 ttatacgctg cattcacagg gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat
    17161 cggcgcgggt ccggtgaaag tcatccatga catcagcgaa atgaaccgca tcgaacctgg
    17221 cgacgtgctg gttactgaca tgaccgaccc ggactgggaa ccgatcatga agaaagcatc
    17281 tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact
    17341 gggcattccg gcggtagtgg gctgtggaga tgcaacagaa cggatgaaag acggtgagaa
    17401 cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc tatgcggagt tgctggaatt
    17461 tagcgtgaaa agctccagcg tagaaacgat gccggatctg ccgttgaaag tgatgatgaa
    17521 cgtcggtaac ccggaccgtg ctttcgactt cgcctgcctg ccgagcgaag gcgtgggcct
    17581 tgcgcgtctg gaatttatca tcaaccgtat gattggcgtc cacccacgcg cactgcttga
    17641 gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc gagatgatga aaggttttga
    17701 ttctccgcgt gaattttacg ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc
    17761 gttttatccg aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa
    17821 cctggtcggt ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg gcttccgtgg
    17881 tgcgggccgc tatgtttccg acagcttccg cgactgcttc gcactggagt gtgaagcagt
    17941 gaaacgtgtg cgcaacgaca tggggctgac caacgttgag atcatgatcc cgttcgtgcg
    18001 taccgtagat caggcgaaag cggtggttga agaactggcg cgtcaggggc tgaaacgtgg
    18061 cgagaacggg ctgaaaatca tcatgatgtg tgaaatcccg tccaacgcct tgctggccga
    18121 gcagttcctc gaatatttcg acggcttctc aattggctca aacgatatga cgcagctggc
    18181 gctcggtctg gaccgtgact ccggcgtggt gtctgaattg ttcgatgagc gcaacgatgc
    18241 ggtgaaagca ctgctgtcga tggctatccg tgccgcgaag aaacagggca aatatgtcgg
    18301 gatttgcggt cagggtccgt ccgaccacga agactttgcc gcatggttga tggaagaggg
    18361 gatcgatagc ctgtctctga acccggacac cgtggtgcaa acctggttaa gcctggctga
    18421 actgaagaaa taaaataaat ccccggcggc gttcagtcgc cggggttatg tgatccccga
    18481 agatgaaact tattcaatct cttcacagac atcctgcgtt aatcgccgta taatatcttt
    18541 tcttaacaaa aacttttgta ttttatctga ggcagtgcgc ggtagttttt cgattaccac
    18601 gatatgttca ggatatttat attttgcgac ccgtttacgg ctaaaaaaag ccgccatctc
    18661 ttccagcgat aatgaatgat gcggcgcttt cagcacgaca taagcgcatg atcgttcacc
    18721 taaacgttca tcgggcattg caaccacaca ggcatcgtga attttaggat gctgcaataa
    18781 aatatcttcc acttcacggc tgctaatatt ttcgccgccg cggacaataa tatctttttt
    18841 gcgtccggta atttttatat agccagcctc atccatacgg cagagatcgc cgctgtaata
    18901 ccagccttct tcatccagcg cacgggcggt taattcaggt tcatcaaaat accccataaa
    18961 cacattgggg ccacgcgatg cttcttcacc ttcgcaacct ggcggtaagg tcttgcgtgc
    19021 gtcatcgacc actttaatct ctacacctgc ggcagcgtaa ccatcggtat gcataaagcg
    19081 cgacaaagaa ttatcgagat tcaccaccgc atgcggcgaa ctttctgtgg aaccataaac
    19141 acttaataat ttaatgctgc gctgctggca ttcgcgcgcc acttttttgg ggattgtggt
    19201 accgccgcaa agaaagaagc gcagcgctga aaggtctgcg ggttgtttct ctagtaaatt
    19261 caaaagatca tagacaaacg gcgttgcgcc tagcatacag gtgcaacgct gctgctcaag
    19321 cagcgcgaga cacgcatcag gagtgaaaat atctaacaac acgctgcgag cgccaattaa
    19381 gaatggtgcc gttacgccat gcagaaagcc cgttgcgtga ccaagtggcg caggcatcat
    19441 aaagacatcc agccaggtca gattcagccg cgcgcaataa gcccgctcac tggcgagaat
    19501 attgttatgc gttagcatca cgccctttgg cagaccctcg gttccggagg taaacagcac
    19561 cgcagctaat tcatcgccgt gggtcgttat cgccgtggtc agtgggatat tgtcggcgat
    19621 aatctgactt aatgagaggg aagaggtggc gggagccagt ttgtccacgc cgacaatttg
    19681 ttgtagttgt ggaagctgat tttgcagcgg caggattaaa tctaccggac gcgtttgttt
    19741 aaacaacgtc ggtgcaaaga acatttttgc ctgacactta ttgagcaccc acaccagttc
    19801 tgcttcccgc caggaaggca acagcggcac ggaaaccgca ccgattttca ggcaggcaag
    19861 atagataacg gtaaattcac accagccagg cagttgaaat gcgatgcgat cgcctgattc
    19921 aatacccttc gctaacatcc agtttgccag acagctcgcg gcgtgatcga gcgcgctata
    19981 ggtgtacgat gcaccatgat tatcgaccac ggcaattttg tctggcatcg cacgagcggt
    20041 ctgctgccag taatcggcca gtgaagcatc gccccatagc ccttgctgac gatacgccgc
    20101 acgacgttgt tcgttaaacg ttaatgtcac tttcattttg gactctcgaa acagaacatc
    20161 aggcccgagt tgcgggcctg tgggatgcat gattagccgt agcgaaaatc aataccgaag
    20221 gtgcctgcgg gatattgcca ctgttcgaga atagtgttac cgcacagcac ccgacaggtg
    20281 ccgcactcca gacaaccggc gggatcaaaa tgaatgtttc ctacgtcatc ctgcttataa
    20341 agtccggcag ggcaggcttt cattaattta cggaattcat tgatatcggg attttccgtc
    20401 agaatgatat gcgggtggcc ctcatcaaca tggaatttat tgacgcctaa tttgatgtcg
    20461 acgttaaccg tagcgttctg gctcatagcg cggttgctcc cttaatgcca tctttcagca
    20521 agttgatcag cccaattttc ttcgcgtgtc ccatgatcat tttgcgtacc ggctggtttg
    20581 gtttgccgtc aatggtgaac atctcgttca tgatgtcggc taccattcgt gggtattggc
    20641 taaacaggcg cgggttttcc atcagcgccg ggatcttgcg aaaatgctgc atatcacgca
    20701 tgacgcagct ttgttccagc tcacgtttgt attgcgccag actgttggcg gagaaatccg
    20761 cgcgttcttt ggcggcgatc accgttgtgg cggcagcctg agccgatgca atggctaaat
    20821 ccatgccgcg gaccgtaaaa cccaaattca ggcagaagcc tgcggcgtca ccaacgatca
    20881 tcacgccctc gttaaccagt tgcggcacca ttgccagacc gccttccggc accatatgcg
    20941 cggaatattc aagcagtttg ccgccgctaa tcagcgggcg aatggcgggg tgttgtttaa
    21001 aatcttccag catttgcggc acgctttttt gcgcatgggc gatatcaccc aatccacaaa
    21061 ccagccccaa ggatatggaa tccttgttgg tatagagaaa tcccccgccc atcaggccgt
    21121 cagaagggga accggcaaac agccaggcgg caccttcctc gcccgtaata ttaaagcgat
    21181 cgttgatctg ttctggtgtg aggccaataa cctctttaac accaacagcg taatgatgcg
    21241 gatcggaagc gggaaccatt cccagcgagc ggccaagcat cgagttaacg ccatcagcca
    21301 gaatcaccac attcgcttcg agaatatcat ccccagcctg cacgccagtg accttgtttc
    21361 cttcacgaac cagcgcgtcg acgcgaacgc ctgggataaa ctgcgcgccc gcctgctcgg
    21421 cttgttccat caaccacggg tccagacgat tacgcaatac ggtataagat gcgtgttgag
    21481 gaacatctgg ttgctcgcgg tgaaaatcga gggtaacggc gctttcttcg gttaagaagg
    21541 agattttctc gcgtgtgacc ttgcgttcta ccggcgctga tactgcaaag cctggaatga
    21601 ttgcttcaag tgtgtgggca taaagacgcc cgccggtcat gtttttacat ccggcactgt
    21661 cgccgcgttc tatcaccagg acatccagcc cggctcgtgc catgaccagt gcggcaacgc
    21721 taccagcaac gcccgcaccg accacaatgg catcaaattt gtcatccgac atgcgttatg
    21781 ctcccttcct gctgcgttta cagcgggaga tgtggttgaa aggggaatta gcggctcaac
    21841 tggctaatca gggcagggac gactttgtag atatcgccca ccagaccgta gtcggcatag
    21901 ttgaagattg gcgcattttt atctttattg atggcgacaa tcacttttac gccgttgccg
    21961 ccaaccatat gttggatctg cccggagatc cccagcgtca ggtagagatc ggatttcagc
    22021 aacacgccgg agacaccgat ataacgttca cgctccatcc agttctcgcc ttcggcaatt
    22081 ggacgtgaac agccgacttc agcattcagc accgccgcca gttcgtggac cattttcagg
    22141 tcatcctgcg ccgccagacc acgaccgacg ccaaccacac gttttgcttt gctcaggtcc
    22201 acgctgcttg cggctttcgc acggcgttcg cgacagagaa tttcatgacg cggagcaaca
    22261 taaggtaccg tttctgtcgg gcactgatga gaggtatcac tggtgcacgg ttcctgaaca
    22321 ccgggtgcaa gggtaatgat cgccagcggg ctgttgatct tttcctgagc gaacgctaac
    22381 ccgccataca tccggtgttc ggcgcaaatg tgaccatcga caatatccac cgccgtggca
    22441 tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg ctgccagagc tttaccacgt
    22501 ttcgtcgcgg ccaacagcaa catagcgggg tgtttatctt tcagcagggc agcaatgctt
    22561 tcggcgtaat tttcagtgcg ttgcagcgcg tcgttttgct caagaacata aatacatttt
    22621 ggaccataag gcataactgc ctgcgcctgg tcggtatttt gtacaatggc atacacctgt
    22681 tggccccatt gctgagcgcc gccaaacagt tcagcataac gttcaggatt atcgctaaag
    22741 acccagacgc tgtttaattg actcatcgtt tcttctccgt aggcttcagt tcagggcttt
    22801 cttcagatgt tccgccagct cggcaatggc ctccggcgaa tcgttatcga gaatgatgtg
    22861 cttacgttct gtttgcggcg gtacgcgaat gccgaccagt tcagcaagtg gcgcgctctg
    22921 gctccagtca atatcacttg cctgccactg atttaccggt tttttacccg cgccgagaat
    22981 ggctttcatc gaaggaatgc gtggcacgtt aatatcggag gtgacgcaga gcacggctgg
    23041 aacagagagt tcaataactt caacatcatc ttcaagcgtg cgttcaatca ccagtgtatt
    23101 gccctgacgc tgaatagcac tcactgcatt aatcaccgga agttgcagaa tttctccgac
    23161 cagcaagcca acctgctggg cataaaggtc gccggaacct tcaccaaaga tcagtaaatc
    23221 gaagccgatc ttttcaattg ctgccgccag cgcttttgcg gtatcgagag gcagtgcatg
    23281 ttcaagttgc gcatcctgca ccaaatacag gctgtgcggc ccgcgggata gcacgtcttt
    23341 gcgcactttc gagttctgca acaatgagcc gccaacggtc agcgcggcta tctcatcgtc
    23401 atctgttgcg agctggcttg cagcttcaat ggcattgaga tcgaactggc tgattttggc
    23461 gtcggcattg tcgaaattca gggtgtattc tggagtgaca acaatgtcct gttcttcagg
    23521 caccagctta aagcaggtta ttattttcat gatttctcct gtgagtcgca tacggaacca
    23581 tctccgatgg aaggtaaaaa tggcctgaaa atagtgtgcg atagcggtgc gggggggggg
    23641 gcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg attgctaaaa
    23701 caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg cattcagtgt
    23761 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
    23821 tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc
    23881 tgttttgata atgccagtga aacgctgttt gtcgccggta aaacgccacg gctttcacgt
    23941 tttgcattta gcgatgatcc aaaatgggag tctggacatc acgttcatga caatgaaacc
    24001 gagctgattt acgtcaagaa aggggttgca aggtttacca tcgattcttc gttatatgtc
    24061 gcacatgcgg atgacattgt ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac
    24121 gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct
    24181 gaggaaaatc agttactgca accgcattct tgtccggtaa ttgccgcggg gcagggaaaa
    24241 gaagtcataa aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
    24301 caaacatctt cgttatggga tgcattagcc tatacattag c
//
LOCUS       DAJTRN010000060        24183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRN010000060 DAJTRN010000000
VERSION     DAJTRN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(22..594)
                     /locus_tag="OO328_002464"
     CDS             complement(22..594)
                     /locus_tag="OO328_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5930995.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO328_002465"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO328_002465"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5930996.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO328_002466"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO328_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5930997.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO328_002467"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO328_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5930998.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO328_002468"
     CDS             complement(3556..5337)
                     /locus_tag="OO328_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5930999.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO328_002469"
     CDS             complement(5330..7102)
                     /locus_tag="OO328_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5931000.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO328_002470"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO328_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5931001.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO328_002471"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO328_002471"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5931002.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO328_002472"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO328_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO328_002473"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO328_002473"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5931003.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO328_002474"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO328_002474"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5931004.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO328_002475"
     CDS             complement(11658..12029)
                     /locus_tag="OO328_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5931005.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO328_002476"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO328_002476"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5931006.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO328_002477"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO328_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5931007.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO328_002478"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO328_002478"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5931008.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO328_002479"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO328_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5931009.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO328_002480"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO328_002480"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5931010.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO328_002481"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO328_002481"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5931011.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO328_002482"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO328_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5931012.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO328_002483"
     CDS             21800..22378
                     /locus_tag="OO328_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5931013.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO328_002484"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO328_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5931014.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6664 c         5827 g         6025 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 ata
//
LOCUS       DAJTRN010000061        23515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRN010000061 DAJTRN010000000
VERSION     DAJTRN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..609)
                     /gene="rluF"
                     /locus_tag="OO328_002485"
     CDS             complement(<1..609)
                     /gene="rluF"
                     /locus_tag="OO328_002485"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5931015.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKL"
     gene            957..1904
                     /locus_tag="OO328_002486"
     CDS             957..1904
                     /locus_tag="OO328_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5931016.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            1974..2780
                     /locus_tag="OO328_002487"
     CDS             1974..2780
                     /locus_tag="OO328_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5931017.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            2790..3197
                     /locus_tag="OO328_002488"
     CDS             2790..3197
                     /locus_tag="OO328_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5931018.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3197..3691
                     /locus_tag="OO328_002489"
     CDS             3197..3691
                     /locus_tag="OO328_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5931019.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            3757..4554
                     /locus_tag="OO328_002490"
     CDS             3757..4554
                     /locus_tag="OO328_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5931020.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4565..5353
                     /locus_tag="OO328_002491"
     CDS             4565..5353
                     /locus_tag="OO328_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5931021.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5405..6634
                     /gene="sorE"
                     /locus_tag="OO328_002492"
     CDS             5405..6634
                     /gene="sorE"
                     /locus_tag="OO328_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5931022.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7016..7705
                     /gene="pepE"
                     /locus_tag="OO328_002493"
     CDS             7016..7705
                     /gene="pepE"
                     /locus_tag="OO328_002493"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5931023.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7796..9427)
                     /locus_tag="OO328_002494"
     CDS             complement(7796..9427)
                     /locus_tag="OO328_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5931024.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9647..13330)
                     /gene="metH"
                     /locus_tag="OO328_002495"
     CDS             complement(9647..13330)
                     /gene="metH"
                     /locus_tag="OO328_002495"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5931025.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13530..14354
                     /gene="iclR"
                     /locus_tag="OO328_002496"
     CDS             13530..14354
                     /gene="iclR"
                     /locus_tag="OO328_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5931026.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14671..16855
                     /gene="espL4"
                     /locus_tag="OO328_002497"
                     /pseudo
     CDS             14671..16855
                     /gene="espL4"
                     /locus_tag="OO328_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(16824..18561)
                     /gene="aceK"
                     /locus_tag="OO328_002498"
                     /pseudo
     CDS             complement(16824..18561)
                     /gene="aceK"
                     /locus_tag="OO328_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(18745..20049)
                     /gene="aceA"
                     /locus_tag="OO328_002499"
     CDS             complement(18745..20049)
                     /gene="aceA"
                     /locus_tag="OO328_002499"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5931027.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20079..21680)
                     /gene="aceB"
                     /locus_tag="OO328_002500"
                     /pseudo
     CDS             complement(20079..21680)
                     /gene="aceB"
                     /locus_tag="OO328_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(21949..22878)
                     /gene="metA"
                     /locus_tag="OO328_002501"
     CDS             complement(21949..22878)
                     /gene="metA"
                     /locus_tag="OO328_002501"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5931028.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23035..23478
                     /locus_tag="OO328_002502"
     CDS             23035..23478
                     /locus_tag="OO328_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5931029.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5809 a         5991 c         5969 g         5746 t
ORIGIN      
        1 cagctttttc acttcatagc cgaaatgctc gcacatgcgc cggatctgac ggttcagccc
       61 ctgcactagg gtaatgcgga agacaaacgg cgcttctttt ttgactttgc actttttcgt
      121 caccgtcccg aggatcggca cccccgcccc cataccacga ataaattcat cggtaatcgg
      181 tttatcgacc gtcaccagat actctttctc atgatcgtta cccgcacgga ggatcttatt
      241 caccagatcg ccgtgattgg tgaggaaaat cagcccctgg gagtctttat ccaggcggcc
      301 aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc gctcgccatc
      361 ttcggtggtg ctgacaatac cgacgggctt gttcagggcg ataagtacca aatcttcggc
      421 ttcccgaggc tcaatcaact gaccgtttac tttcacaatg tcgccgggtt tcacctgatc
      481 gccaatggtg gctcgcttgc cattaaggaa cacattgcct tgctcgatat agcgatccgc
      541 ttcgcggcgt gagcaaattc cgctttcgct gatgtattta tttaaacgga ctgatgagtc
      601 gggcagcata aattctcctg taaaacgcgg aatataccgt acctcttagc ggggaaaaac
      661 agggttatgc ggcaacgtcg aaattaaatg tgatctgcca cgccttttat cacaaaagtg
      721 tctccggaag tacgatgcaa atttgcctgg caaaataagg ataatcaaca tattacgaat
      781 cgctgcacac ctgtgctgat gactactacg cagagaaaca aatgtgcaag agaaagcgct
      841 atttctcccc atgcaaagag tgaaaaatcg ctttgaaagg ccctcatgat ggggttatat
      901 cagatcaagc ggtgagaaag cgtatttgca caaatgtgca gaaccaggag gcggcaatgg
      961 aaaacagtga cgatatccgt ttgattgtga agattgccca actctattac gaacaggata
     1021 tgacgcaggc gcaaatcgcg cgcgaactgg gtatttaccg caccaccatc agccgcttgc
     1081 ttaaacgagg ccgcgatcag ggaatggtca ccatcgccat caactatgac tacaacgaaa
     1141 atctctggct ggagcagcaa ctgaagcaaa agtttggcct gaaagacgtt gtggtggtgt
     1201 cgggaaatga tgaggatgaa gagactcaac tggcgatgat ggggttacac ggcgcgcaac
     1261 tgctggatcg cttgctggag cctggcgata ttgtcggttt ttcctggggt cgcgcggtga
     1321 gcgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg gcagttaatc tgcgtaccaa
     1381 ttattggcgg accgtccggt aaactcgaaa gccgctatca tgtaaacaca ttaacctaca
     1441 gcgcggcagc gaagctgaaa ggggaatcgc atctcgcgga ttttccggct ctgctggata
     1501 acccattaat tcgtaatggg atcatgcagt ctcagcactt taaaaccatc tctgcctact
     1561 gggataatct ggatatcgcc ctggtgggaa ttggctcacc ggccattcgc gacggcgcta
     1621 actggcatgc gttttatggt ggtgaagaga gtgacgacct gaatgcccgc caggttgctg
     1681 gcgatatttg ctcgcgcttt tttgatattc acggcgcaat ggttgaaacg aatatgagcg
     1741 aaaaaacgct ctctatcgaa atgaataaat taaagcaggc gcgatattcc attggcattg
     1801 ccatgagtga agaaaaatac agcggaatta ttggtgcact gcgtggaaaa tatattaatt
     1861 gtctggtaac gaatagcagc acagctgaac tattactgaa ataaaaatct tcccgatgtt
     1921 taccgtaata aaaattacgg cgggtcccta ttatctaaaa tcaggagtct gttatgcaaa
     1981 cgtggttaaa tttgcaggat aaaatcatta ttgtcaccgg cggcgcatcc ggtattggtc
     2041 tggcgattgt agaggaatta ttagcacaag gcgcgaatgt acagatggtc gatattcacg
     2101 gtggcgatgg tcaatatgaa ggtcataaag gctatcagtt ttggccgacc gatatttcta
     2161 gcacaaaaga ggtaaatcat acggtagcgg aaattatcca gcgttttggt cgcatcgacg
     2221 gtctggtcaa taacgccggg gtcaatttcc cgcgtctgct ggtcgatgag aaagcgcctg
     2281 ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat ggtcaatatc aaccagaaag
     2341 gtgtttttct gatgtcgcag gcggtggcgc gacagatggt caaacaacat gatggcgtga
     2401 ttgtgaatgt ttcctcagaa agtgggctgg aaggctcaga aggacaaagc tgttacgccg
     2461 ctaccaaagc cgcgctcaat agcttcacgc gctcctggtc gaaagagctg ggtaaacacg
     2521 gtatccgtgt ggtcggtatc gcgcccggga ttctggaaaa aaccgggcta cgcacgccgg
     2581 aatatgaaga ggcgctggcg tggacgcgca atatcaccgt cgagcagctg cgtgaaggct
     2641 ataccaaaaa cgccattcct attgggcgcg ccggaagatt agcagaagta gctgattttg
     2701 tttgttatct gttgtctgaa cgcgccagct acatcaccgg agtaaccact aacattgcgg
     2761 gcggcaaaac gcgcggctaa ggaggcttta tggtcaacgc cattttttgt gcccacggca
     2821 aattagcttg cgccatgctg gaatcggttc agatggttta cggcaacgcc aatgttgaag
     2881 ccgttgcatt tgtgcccggt gaaaacgccg gagacattgc ggtaaagctg gaaaagttag
     2941 taagcattca caatcaagat gagtggctga ttgccgtgga tttacagtgc ggcagcccgt
     3001 ggaatgcggc agcaatgctt gcgatgcgca atcctcgttt gcgggtaatc agcggccttt
     3061 ctctgccgct ggcgctcgaa ctggttgata accaggacag catgaatgtt gatgaactgt
     3121 gtgaacacct gacgcaaatc gcaaagcaaa cctgcgttgt ctggaaacaa ctggcaacga
     3181 cagaggagga tttctgatga acattacgct cgcccgcatt gatgaccgtc tgatccacgg
     3241 ccaggtcact accgtctggt cgaaagtggc taacgcccag cgcattatta tctgcaatga
     3301 cgaagtttat aacgatgaag tgcgccggac attattgcgt caggctgcgc caccgggaat
     3361 gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt tatcacaacc cgcaatatca
     3421 ggacgaaacc gttttttatc ttttcactcg cccacaggat gccttagcga tggttcgcca
     3481 gggggtaaaa ataggcaccc tgaatattgg cggcatggcc tggcggcccg gtaaaaaaca
     3541 gttaaccaaa gcggtttcat tagatgatga tgatattaac gcatttcatg agttaaataa
     3601 tcttggtgtc atactggatt tacgcgtcgt agcgtcagat ccctcaatca atattatcga
     3661 caaaataaac gaacagttaa tcgcaaatta aataatagcg cctgtgtaca tatgctttaa
     3721 caggcaggga tgacgacact taaaaggtga caaatcatgg aaataagtac cctacaaatc
     3781 attgctatat ttcttttttc ctgtattgcc ggaatgggca gcgtgctgga tgaatttcag
     3841 actcatcgcc cgttaattgc ctgtacggtg attggtttaa ttctcggtga tttaaaaacc
     3901 ggaataatac tcggcggtac gctggaattg atagctctct gctggatgaa cgtcggcgcg
     3961 gcgcaatctc cggattctgc actcgccagc ataatctccg ccattctggt tatcgttggt
     4021 cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg tggctgcggc aggccaggtg
     4081 ctgacggtgt ttgcccgtac catcaccgta gtgttccagc acgcggcgga taaagcagca
     4141 gaagaggcgc ggtttcgcac tctcgatatt ctgcatgtct ccgcgcttgg cgtgcaggcg
     4201 ctgcgcgtcg ccattccggc actgattgtc tcactgttcg tcagcgccga tatggtcagc
     4261 aatatgttaa acgccattcc cgaatttgtg acccgaggac tacagattgc tggcggtttt
     4321 atcgtggtgg tcggttacgc catggtgcta cgtatgatgg gcgtgaaata tttgatgcct
     4381 ttctttttcc tcggtttcct cgcaggtggc tacctcgatc tcagcctgct ggcgttcggt
     4441 ggcgtcggcg tgatcatggc cctggtctac atccagctaa atccacagtg gtgtaaagct
     4501 gaacctcaac tccaggccac cgcctccacc gcccttgacc aacttgacga ttaacggagc
     4561 catcatggaa cagagaaaaa ttacacgcag cgatctggtg agcatgtttc tgcgctccaa
     4621 cctgcaacag gcgtccttta actttgaacg tattcacggg ctgggctttt gctacgacat
     4681 gatccccgcc atcaagcgac tttacccatt aaaagaggat caggttgcgg cgctcaggct
     4741 acacctggtg ttcttcaata ccacgccagc cgtatgtggc ccggtcatcg gcgcggaaat
     4801 tgatgacggt accatcaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg
     4861 cgatccactg gtctggggaa cgctgcgccc gattaccgcc gcgctcggcg catctctggc
     4921 actttcgggc aacattctcg gcccgctgct gttcttcttt attttcaacg cggtgagtct
     4981 ggcgatgaag tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga
     5041 tatgggcggg aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt
     5101 gatgggcgtg ctggtgacca aatggacgtc aatcaacgta ccgttggtgg tttcacaaac
     5161 gcatgccgcc gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg
     5221 ccctggtttg ctggcgctcg gtctgacgct actaatggtt cgtctgctca acaaaaaaat
     5281 taacccggta tggctgattt tcgccctgtt tggcttaggg attatcggca atgctctggg
     5341 cttcctgtcc tgattcttcg ccccggcacg actgccgagg ccatcgctca acatgaggtg
     5401 gtttatgaaa acaacagctc tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt
     5461 tgaccttcct gaaatgcagg aggatgaaat cctcgcaacg gtggtcactg acagcctgtg
     5521 cctctcttcc tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt
     5581 gacgaccaac ccaatcatca tcggccacga attttgcggc gatattctgg ccgtgggtaa
     5641 aaagtggcag cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact
     5701 ccccgaccgc ccggattgcc ccggctactc cttcccgtgg gtagacggcg aagcgacgca
     5761 tgtggttatt cccaacgagg tcatggaaca atattgcctg ctggcatacg acggcgaaac
     5821 ctattttgaa ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa
     5881 ctatcatctt caggaaggta gttataacca cacgatgggg attcgcccgc aagggcacac
     5941 gctgatcctc ggcggcaccg gaccaatggg actgttggcg attgattatg cgctgcatgg
     6001 accagttaac ccgtcgctgc tcgtcattac cgataccgac aacgacaaat tgagttatgc
     6061 gcgcaagcac tatccatcag aaccgcaaac actgattcat tatctcaatg ccaccgatgc
     6121 agcatttgat acgttaatgg cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt
     6181 tgtgcctaat gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgcct
     6241 gaatttcttc gccggaccgc aggataaaca tttcagcgcg ccaattaatt tctacgatgt
     6301 gcattatgca tttacccact acgtgggcac gtcaggcggc aataccgacg acatgcgcgc
     6361 ggcggtcaaa ctgatcgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct
     6421 tgggctgaat gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa
     6481 gctggtgtat accgggaaat acctgccgct gacgtcactc acgcagattc aggatcaagc
     6541 actggcggcg attctggcgc gtcatcaggg gatctggtcc ggtgaggtgg agcaatacct
     6601 gctcgcccat gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc
     6661 tggctggtcg ccccagcaac ttcgcgaccg gatgtgcggc tgattgctga agcagcacaa
     6721 tttgcacgag aaggataaaa aatatcagcg ccttaagggg cgctcaaatt ggtggcaaag
     6781 tacacattgt ttatgccgga tgcggaatga agccttattc ggtctacata attttgaaaa
     6841 tccaatatat tgcagttatc tggtaggcca ggtaagcgta gcgcatcagg caatgctgtg
     6901 tttgtcatca gtctcagcgc ccggtgcaat acgtgacatc gtgagccatg tcagtaagtg
     6961 gttaaattcg cagtggtagc atgccttccg tgatttctta ctaaccggag aagtaatgga
     7021 actgctttta ttgagtaact cgacgctgcc gggtaaagcc tggctggaac atgcactgcc
     7081 gctaattgct gaacagttgc agggtcgccg ctcagcggtg tttatccctt tcgctggcgt
     7141 aacgcagacc tgggatgatt acacagagaa aacggctgcg gttctcgctc cgctgggtgt
     7201 ttctgtcacc ggtattcata gcgttgtcga tcccgttgcc gcgattgaaa atgcggaaat
     7261 tgtgattgtc ggcggcggta atactttcca gttgctgaaa cagtgccgcg agcgcgggct
     7321 gctggctcca attactgacg tggttaaacg tggcgcgctg tatattggct ggagcgcagg
     7381 cgctaacctt gcttgcccaa ccattcgtac caccaacgat atgccgattg tcgatccgca
     7441 aggtttcgat gcgctaaatc tgttcccgtt gcaaatcaac ccgcacttca ccaacgcgct
     7501 gccggaaggc cataaaggtg aaacccgtga gcagcgtatt cgcgaactgc tggtcgtcgc
     7561 gccagaactg acgattattg gtctgccgga aggtaactgg atcacagtga gtaaaggtca
     7621 cgctacgctg ggtggcccga acaccactta tgtgtttaag gctggtgaag aagcggttcc
     7681 gctggaagct ggtcaccgtt tttaagtgaa ttgccggatg catgatccgg catcttgttc
     7741 atagtgcctg atgcgacgct tgacgcgtct tatcaggcct acaggattca caatattaat
     7801 actcgtcccg tccttcatct tcatccggct gttccagcac actgtaagcc accgaacaaa
     7861 acagcgagtt caggcgctgc atatctccca gtaagcctaa atgtagcgaa ctggtttcaa
     7921 tgctctgcac gttttgctga tgcaggcgat cgacgtgggc gtgggaatag cggcgattaa
     7981 gaatgcgaaa acgatgtttg ctgcgacgca aacgacgagc gctggtgaca tcgccagaga
     8041 agaacaccga cattgccagc tttaaattac tgagcaattg ctcatagagc gcatccagtt
     8101 ccttcaaccc atcaagcgaa aatgcccgcc gtgctgccag cgatttatcg gcaatttcgc
     8161 tgcccatgcg ctcgacgata tcggaggcct gttcaaggtt gagcgacatt tcgatgatct
     8221 ccgcccagcg gcgcgactct tcctccgcca actcttcttt tggcatccgc gccagataca
     8281 gcttaatggc ggtgtagaga acgttgatat catctgccag cttacgcagc tctttctcct
     8341 gccgtggctc gccgtgcatc actttattca gcccttccat catctgttcc atggcgtcgc
     8401 caatgcgcag ggtttcgcgc gcggcgttcg ccagagcaag cgtgggcgtg tccagcgcgc
     8461 tgacatccag atgtttaggc cgtagctggg tatccagttc cggttcatcg cgaataatcg
     8521 ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat gaccaggcaa cgtacaaggt
     8581 tgtagaagac gtggaaatag atcaccagtt ccgcttttgg caatgacagc ttccccattg
     8641 tctctgccag caaatggaca aacggcagga taatcaggct acccaccagc ttaaacagca
     8701 gactacccag cgcgacacgg cgggctgcgg cattggcggc actgttgttg agcatcgcca
     8761 gcaggccgga accgaggtta gctccaatca ccagacagag cgccacgggg aaggagataa
     8821 tgcctgcagc ggtcagagtc gcggtcagca gtacagcagc aaggctggag tagctgataa
     8881 tggcgaacat cgcgccaatc agcgcatcca gcagaatatc gccggtcagc gaggcaaaga
     8941 tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc ctgcacaatc aactccagcg
     9001 ccagcaaaat cagcccaaga ccaataccga cgcggcccag ttgcccggcg cgtgactgtt
     9061 tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa cggtgacagc caggataagt
     9121 cgaaggtgag aatacgcgcc attagcgccg tcccgacatc tgcccccagc acaatgacca
     9181 gagccggtgc gagggctacc agatcctgag cgacgaacga ggtcaccagc atggtggtgg
     9241 cattactgct ctgtaccagt gcggtaacgc cgatccccgc gcaaaaggcg agcggctttt
     9301 tttcgacgct ccggctaagg acagtacgca aacgcgcgcc gaagacgcgc attacgccgg
     9361 ttcgaacaat atgagtcccc cagaccagca gggcgacggc agaaagcagg tgaagcagcg
     9421 ttaacacgag ttcagattct ccttatcgtt atatgttcct gaagcaattg aatgaccaaa
     9481 ggaccggaaa cacgctgact ggcatcttta ctgtgccttt tgacattcgt ttttccggct
     9541 aagattattc cagcctgttt ccagtataag tgtttcatat taataaagag acagggcact
     9601 cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt tgtgaatcag tccgcgtcat
     9661 accccagatt cggtgccagc cagcgctcaa catcggagac gctcatacct ttacggcggg
     9721 catggtcttc aacctgatcg cgctgaattt gcgccaccgc gtagtacttg ctgtccggat
     9781 gactgaaata ccaacccgaa accgatgcac caggccacat cgcgaaagat tctgtgagtt
     9841 tcatgccagt atgtttttcc acttccagca gctcccagat ggtggctttt tccgtatgtt
     9901 ccgggcaggc cggatagccc ggtgccggac ggatgccctg gtagttttcg cggatcagtt
     9961 cttcgttgct gaggttctcg ttcggcgcat agccccagta gactttacgc acacgctcat
    10021 ggagatactc cgcaaaggct tcggctaaac ggtcggcaag cgctttcacc atgattttgt
    10081 tgtaatcatc gtgctgcgct tcaaaggcat cagccagtgc gtcctcttcc agcccgccag
    10141 tcacggcaaa tgcgccgatg taatctgctt taccagaaag cttcggcgca acgaagtcag
    10201 cgagacagta gttagcgaat cccgtttttt cggtctgttg acgcagatgg tggctgacgt
    10261 tgatcacatg ggtgcgcgtt tcgtcacggt agatttcaat gtcatcgccc acacggtttg
    10321 ccgggaacag gcccaccacg ccacgcggat tcagcgtttt ctcggcgctt aatttatcca
    10381 gcatgtcgtt ggcgtcttta aacagccgct gcgcctcaac gcccaccact tcatcttcca
    10441 gaatgcgagg atacttcccg gccagcgacc aggtcataaa gaacggtgtc cagtcgatgt
    10501 aattacgcag cgtttcgatg ctggcttcca cttcctgcac gccgagacgg tgcgccaccg
    10561 gcggcgtgta agcctgccag tcaaatgcga aatcgttatc gcgcgccgct tccagcgtga
    10621 ccggtggtgt gcgtggtttc ttgcgcccgt gctgaatacg cacggtttcg tactccttgc
    10681 aggtacgagc gacaaaatca tcacgctgag tatcggaaag cagcgccgct accacaccaa
    10741 cggtgcgcga ggcattctgc acatacaccg tagggccgct gtagttctgc tcgattttca
    10801 ccgccgtgtg cgcttttgag gtggtcgcgc cgccaatcag taacggaatg gcgaagccct
    10861 gacgctccat ctctttcgcc acgttaacca tctcgtccag cgacggcgtg ataagccccg
    10921 aaaggccaat caaatcagca ttcacttctt tggcgatacg gagaattttt tccgcaggca
    10981 ccataacgcc gagatcgaca atttcgtagt tgttacattg cagcaccaca ccaacgatat
    11041 ttttaccgat gtcgtggaca tcgcctttca cggtggcgat caccatcttg ccgttggttt
    11101 taccctgctc tttgctggct tcaataaacg gttcgagata cgccactgcc tgtttcatga
    11161 cgcgcgccga tttgaccacc tgtggcagga acattttgcc ttcgccaaac agatcgccga
    11221 ccacgttcat gccgtccatc aacgggcctt caatcacttc aatcgggcgc gtagcctgct
    11281 ggcgggcttc ttcagtatcc tgctcgataa actcggttat gcctttgacc agcgagtatt
    11341 ccagacgttt attcacgtcc cacgagcgcc actccgcttg ctgggcgtta gcagtgtcgt
    11401 ccgctttact gccgcgatat ttctcggcaa gctccagtaa acgctcagtg ccatcgtcac
    11461 gacgattaag aatcacatct tccaccgcgt cgcgcagttc agcgggtagg tcgtcgtaaa
    11521 tcgccagttg cccggcgttg acgatcccca tatccatgcc attgcgaata gcgtagtaga
    11581 ggaacactgc gtgaatggct tcgcgcaccg gatcgttgcc acggaatgag aaagaaacgt
    11641 tagatacgcc gccggaaatc agcgcgtgcg gcagttcgcg tttgatgtct tcacacgcgc
    11701 cgataaagtc ctgcgcgtag ttgttgtgct cttcaatacc ggtggcgacc gcgaagatgt
    11761 tcgggtcgaa gatgatatct tctggcggga agccaacctc ttcggtgagg attttgtacg
    11821 cccgacggca aatctcgatt ttccgtgcgc gagtatcggc ctgtccctgt tcgtcaaagg
    11881 ccatcaccac cactgccgca ccgtagcgac gcaacaattt cgcgtgatgg ataaaggcgt
    11941 cgacgccctc tttcatcgag atagagttaa caatgccttt gccctggata cacttcagac
    12001 ctttttcaat gacatcccat tttgaggagt cgatcataat cggcacgcga gcgatatccg
    12061 gttcaccggc aatcagattg agaaaacgca ccatcgccgc ttcggcatcg agcatccctt
    12121 catccatgtt gatatcgata atctgcgcgc cgttttccac ctgctgacgc gccacatcca
    12181 gcgcctcgct gtatttctct tctttgatca ggcgcttgaa cttagcagag ccggtgacgt
    12241 tggtgcgttc acccacgttc acaaacaggc tatcgtcgcc aatgttcagc ggctccaggc
    12301 cagacaaacg gcaggctacg ggaatttccg gcagtttgcg cggcgctaat ccttctactg
    12361 cacgactcat cgctgcaata tgttgcggcg tggtcccgca gcaaccaccg acgatattga
    12421 gaaagcccgc ttgcgcccat tcacgtatct gttttgccat cgtgtcggcg tcgagatcgt
    12481 attcaccaaa ggcgttaggc agcccggcgt tcgggtgggc ggtgacgtag cattccgcaa
    12541 tccgtgatag ttcctgcacg tactggcgca gttcatcggg ccccagcgca cagttcaggc
    12601 caaaggtcag cgcttcggcg tggcgcaatg agttgtaaaa tgcttcggtg gtctgcccgg
    12661 agagcgtgcg cccggaggcg tcggtgatgg tgccggagat cataatcggc agctcaacgc
    12721 ccagcgcttc aaactccgtt ttcaccgcaa agaccgccgc tttggcgtta agggtgtcga
    12781 aaacggtttc aatcaggatc agatccgcac caccttccac cagcgctttg gtggattcgc
    12841 gataggccgc cgccagctgg tcaaaagtga tgttgcggaa tgccggatcg ttgacgtccg
    12901 gagaaataga cgccgtgcgg ttggttgggc caagaacacc ggcaacgtag cgcggtttct
    12961 ccggcgtgcg cgcggtccac tcgtcagcac aggcgcgcgc cagtttcgcc gccgcaaagt
    13021 tgatttccgc cgacagggat tccatctggt aatccgccat cgcaatggtc gtggagttga
    13081 aggtgttggt ttcgatgata tccgcgcccg cttcaaagta ggcgttgtgg atagcggcga
    13141 tcacttccgg tttactgagt accagcaggt cgttgttgcc tttgaggtcg catggccagt
    13201 cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg atagctctgg atcatggtgc
    13261 ccataccgcc gtccaacacc agaatacgtt catttaactg cgcacgcagt tgttccactt
    13321 tgctgctcac acttgctccc gacacgctta acccagattt aataaaaatt caacaaacca
    13381 tactggaata aacgcatctg tggtaaaagc gaccaccacg caacatgaga tttgttcaac
    13441 attaactcat cggatcagtt cagcaactat tgcattagct aacaataaaa atgaaaataa
    13501 tttccacgat acagaaaaag gaggctgtca tggtcgcacc cattcccgcg aaacgcggca
    13561 gaaaacccgc cgttgccacc gcaccagcga ctggacaggt tcagtcttta acgcgtggcc
    13621 tgaaattact ggagtggatt gccgaatcca atggcagcgt ggcactcaca gagctggcgc
    13681 aacaagccgg gttacccaat tccacgaccc accgcctgct aaccacgatg caacagcagg
    13741 gtttcgtgcg tcaggtcggc gaactgggac actgggcaat cggcgcacat gcctttatgg
    13801 tcggcagcag ctttctccag agccgtaatt tgttagcaat tgttcaccct atcctgcgca
    13861 atctaatgga agagtctggc gaaacggtca atatggcggt gcttgatcaa agcgatcacg
    13921 aagcgattat tatcgaccag gtacagtgta cccacctgat gcgaatgtcc gcgcctatcg
    13981 gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc ctttttagcc caactgagcg
    14041 aagaacaggt gacgaagctg ctgcaccgca aagggttaca tgcctatacc cacgcaactc
    14101 tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg ggttattcat
    14161 ttgacgatga ggaacatgcg ctggggctac gttgccttgc agcgtgtatt ttcgatgagc
    14221 accgcgaacc gtttgccgca atttctattt ccggaccgat ttcacgtatt accgatgatc
    14281 gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa ggaagtgacg ctggcgtacg
    14341 gtggaatgcg ctgacttttt ctggcgggca gaggcaatat tctgcccatc atacctgagt
    14401 ggcaatagaa taagggtgtc tgttaatcgc attgacgcca aaataactta atgtcataca
    14461 cttcacacac ttttattaac tcagcattat ttttaaacat caaaccactt aattattaac
    14521 aaaatcgcaa gatctcattc caccgaaact tttacggctg cattatgaga ttgagcgatt
    14581 tatgatattt ttttagttgt aatcatatta aattttttca ctgttctgat agttaaaatt
    14641 caagacatca ataaacaatg agatatttaa atgattactc gtattcctcg tagttctttc
    14701 tctgcaaata ttaataatac agcccagaca aatgaacacc aaaccctgag tgaattgttt
    14761 tataaagaac tcgaggataa attttctggc aaggagctgg cgacgcctct attaaaaagc
    14821 ttctcagaga actgtagaca aaatgttcgt catatcttta gcaacaagga ttttgtcatt
    14881 aaattttcca cgtccgtctt acaagctgat aagaaagaaa ttacgatgat taataaaaac
    14941 gaaaacacga cactcactca aaccattgct acaatatttg aagaatacct aatggaaatt
    15001 ttacctcaac gctcagacac tcttgataaa caagaattaa accaaaaatc agatagaaaa
    15061 gaaaaagaat tcccaagaat taaacttaat ggtcaatgtt atttcccggg gcgccccaaa
    15121 accgtatagt ataccgacac attgctgcac aatatattaa tgatatttat cagaatgttg
    15181 attacaaacc ccatcaagat gattactctt cagctgaaaa atttctcact cacttcaaca
    15241 aaaaatgcaa aaaccagact ttggcgttga tttccagccg tcctgagggg cgttgcgttg
    15301 ctgcctgcgg tgatttcggg ctagttatga aagcatattt tgacaagatg gaatcaaatg
    15361 gcatcagtgt tatggcagcc atattactgg tggataacca tgctttgacg gtccggctaa
    15421 gaataaagaa cacaactgaa ggatgtaccc attacgtggt ttcggtttat gatcctaatg
    15481 taactaacga taaaataaga attatgagcg aaagcaaaga ggatattaaa cactattctc
    15541 tgatggattt tatgaatgta gattatagcc tcctgaaatg gtcaaatgat catgttatta
    15601 accaatctgt tgcaataatt ccagcacttc cgaaagaaca gctattgatg ttaaaaggaa
    15661 ctgtggatga aataacccct ccattatcac cagcaacgat gaatttgcta atggcaattg
    15721 gtcagaatca ccaacttacg caactgatga ttcagctcca gaaaatgcca gaactacata
    15781 gaacagaaat gttgactgcc tataatagta ttaacctccc cggtttatat ttggctataa
    15841 attatggcaa tgcggatatc gttgagacta ttttcaattc attgtcagaa acaggatatg
    15901 aagggttact ctcgaaaaaa aatctcatgc atattctgga ggcaaaagat aaaaatggtt
    15961 tttctggatt atttttagcg atatcacgta aggataaaaa tgttgtaacc tcgattctga
    16021 acgtcttacc taaactggcc gcaacacatc atttagataa tgaacaggtg tataaattcc
    16081 tgagtgccaa aaatagaacg tccagccatg ttttatacca tgttatggcg aatggtgatg
    16141 ccgacatgct gaaaattttt ttggtcgcgt tacctttgtt aattcgcaca tgtcatttga
    16201 ctaaagaaca ggtactcgat ctcctgaagg caaaggattt ttatggttgc ccaagactat
    16261 acctggcgat gcaaaatgga catagcgata tcgtgaaagt tattctcgaa gcattgccct
    16321 gtctggccca ggaaattaac atttcagctt ccgatattgt agatcttctg accgctaaaa
    16381 gtcttgcgcg cgacacgggt ttgtttatgg ccatgcagcg cggacatatg aacgttatta
    16441 atactatttt taacgcatta cccactcttt ttaatacgtt taaattcgat aaaaaaatat
    16501 gaagcccctc ctcctggcaa ataattctaa tgaatatccc ggtttgtttt cagcgataca
    16561 gcataaacaa caaaacgttg tagagacggt ttatcttgct ttatctgacc atgcacgcct
    16621 gtttggattt accgctgaag atattatgga tttttggcaa cacaaagcgc cacaaaaata
    16681 ctctgccttt gagttggctt ttgaatttgg tcaccgggtt attgctgaat taatccttaa
    16741 tacattaaat aagatggctg aaagctttgg ctttacggat aaccctcgat acattgcgga
    16801 gaaaaattat atggaagctt tactcaaaaa agcatctccc cataccgtac gctaaatctt
    16861 tgcctgcgcc gatacgcata aacatcttcc acatgccctt cacgtatgcg gttttgtagt
    16921 gcgcgccagt aatcagcgcg gaacaggtcg gcgtgcatct cttcaaacag cggaccaata
    16981 cgcgggtcgg cgcatagcca gtggcgaaac tcttccggga aaacatcgcc cggcgagacg
    17041 ctgtaccacg gttcgctggc aagttcgtct tccggatagc gcggcggcgg gatgtcgcgg
    17101 aaattcactt ccgtcatgta gcaaatttca tcgtaatcat aaaaaaccac acgcccgtga
    17161 cgggtgacac cgaagttttt aaacagcatg tcgccaggga aaatgttagc agcggcaagc
    17221 tggcgaatag cgttaccgta ttcttcaatg gcgtcgcgca actgctgacc ttccacttgt
    17281 tccagccaga tattgagcgg caccatccgc cgctcgatta tagagatggc gaattacaat
    17341 ctgctcacca agatcggtga ttttttccgc tgcttcctgg agcagtaatt ccattaatac
    17401 cggggaaata tgccgcttct ccagcacaaa gttttcaaac tcctgggtgt ccgccattcg
    17461 gcccacacgg tcgtgctctt tcaccagttg atagcaggca cgaacgtgag cggcagacat
    17521 ctctttctgc ggcgcgaact tgtctttgat gactttgaat acccgatcaa agcccggcag
    17581 cgtaaacacc aacatcacca ttccacgaat acccggcgct tcaatgaact gctcattaca
    17641 gccctgtaga taaacgagat attcgcggta gctttcggtt ttggcgtgct tctggcagcc
    17701 gatagccata tacaattcag cagtggtttt acctggcaga atttcccgta gccactcgac
    17761 cagcgctgcg ggcagcggtg cataaaccat aaaataagaa cgcgcaaagc caaaaacaat
    17821 gctcgcttcg gcggtcgtcg tcaggcaggt atcaataaat aactcgccgt cgtccgtctg
    17881 gtggatcggc agtaagaagg gcaacgtgcc ggaaggcgtg atcagtttgc ccaccagcca
    17941 ggcggcttta ttgcggtaaa acagttcgtt cgccacctgt aaatgacttt ccgcgaggtt
    18001 gtctgtcccc agcgtttccg tcagatggcg aatgatgtaa tgaatgtcac ggcttttatt
    18061 ctgccagcgc aggcgcagcg gcaggtcgct gataacgcgc atcagtagag attcccagcc
    18121 gtgatcgggg tgaaagtctt tcgccagcgg gcggggaatg gtacgaaagc ggcgctctgg
    18181 ctgagagcta aagataaaaa gccgctcggg agtaagcgag cggtggtcaa ataaccgaca
    18241 gtacacggag ttaaaaaagc tctccgcaat ctcgaagcgc gggtaatccg gcaacagccg
    18301 cgtgtaatgc tctttaacgc gcagtaaaaa tgccgcgtcc gtactttggc cgttagtaat
    18361 gcagcgcagt tgctccacga ccagaccaac gtgatgatcg taaagatgga tacggttttt
    18421 catcgcctgc tggacagcat gccagtcggc ctgttcgaaa cgctgctgcg ccccggaagt
    18481 cacttcgagg aatcgaccat actgagcgtc gaagccttgc aaaatggttt gagcaatcag
    18541 taattccagg ccacgcggca tttagcgccc tcatcaggag cagagaattg ccggatgcgg
    18601 cgtaaacgcc ttatccagcc tacgttcggc aacggttgta ggcatgataa gacgcgcaag
    18661 cgtcgcatca ggcatcgtgc accgattgcc ggatgcggcg taaatgcctt atccggccta
    18721 cagtcagcaa cgtttgtagg ttgcttagaa ctgcgattct tcagtggagc cagtcagcgc
    18781 ggtgactgaa gacgtgccgc cctgaataat agtcgtcact ttatcgaagt aacctgtacc
    18841 cacttcctgc tggtgagata cgaaggtata gccatctttc gcggcggcaa attccggctg
    18901 ctgcactttc tcaacatagt gcttcatacc ttcgccctgc gcataagcgt ttgccaggtc
    18961 gaacatgtta aaccacatgc tgtggatacc tgctagggtg atgaactgga acttgtagcc
    19021 catatccgac agctgctgct ggaagctggc aatagttttg tcgtcgaggt ttttctgcca
    19081 gttgaacgac ggcgagcagt tataagccag cagtttgccc ggatatttcg cgtggatagc
    19141 ttgtgcaaag cgacgcgcca gttccagatc cggcgtggag gtttcacacc agaccaggtc
    19201 agcatatggc gcatacgcca ggccacggct gatcgcttgc tcaatgcccg catgagtacg
    19261 gaagaagcct tcactggtac gctcgccggt aataaattcg ctgtcatacg ggtcgcaatc
    19321 tgaggtgatc agatccgccg catcagcatc ggtacgtgca accagcaggg ttggaacgcc
    19381 cgtcacgtca gctgccagac gcgccgcgac cagtttctga atagcttcct gagttggcac
    19441 taaaactttg ccgcccatgt gaccgcattt cttcactgac gccagctgat cttcgaagtg
    19501 aactgccgca gcaccggctt caatcatcgc tttcatcagt tcaaaggcat tcaggacacc
    19561 gccaaaaccg gcttccgcat cggcaacgat cggcaggaag taatcgacat agcgcggatc
    19621 gcccggctca atgcccacgg accattggat ctgatcggca cgacggaagg tgttgttgat
    19681 ccgctccacc acagctggca ccgagtttgc cggatagagc gactgatccg gatacatgct
    19741 ggccgccagg ttagcgtccg ccgctacctg ccatcccgac agatagactg cttcaatacc
    19801 cgctttcgcc tgttgcagcg cctgaccgcc agtcagtgca ccgaggttgt tgatgtagcc
    19861 ttttttcgac tcaccgtgca gcagacgcca cattttcgct gcgcccagtt gcgccagcgt
    19921 gcattcagga ttgactgaac cgcgtaattt caccacatct tccgcactgt atgggcgagt
    19981 aatgccttcc caacgcggtt gagtccactc tttctgtaat tcttcaattt gttgtgtacg
    20041 ggttttcatg tgcagatgct ccatagttat gtggtggttt acgctaacag gcggtagcct
    20101 ggcagggtca ggaaatcaat taactcatcg gaagtggtga tctgttccat caagcgtgcg
    20161 gcatcgtcaa aacggccctg ggagaaacgt tcttcgccca gttcgctggc aatgactttc
    20221 atctcttcgc ccagcatctg gcggaacaag gctttggtca ccggtttgcc attgctcaac
    20281 gttttttgat gatggatcca ctgccagatc gaggtacggg aaatttcagc cgtcgccgca
    20341 tcttccatca ggccataaat cggcacgcag ccgttgccgg agatccacgc ttcgatgtac
    20401 tgcacagcca cgcgaatgtt agcgcgcata ccttcttcgg tgcgttcacc atcacaaggt
    20461 gccagcagct gatcggcagt aatcggcgcg tcttgttcgc gcaatacttc aagctgattt
    20521 ttacgggagc cgagaatgtc gttgaatacc gccattgccg tgtccgcaag gcctgggtga
    20581 gcgatccatg tgccatcgtg accgttattg gcttccagcg ctttatccgc ttttactttg
    20641 ttgagcacct ggttattacg ctcttcatct ttgctcggaa taaacgcggc catgccgccc
    20701 atcgcaaaag caccgcgttt atggcaggtt ttaatcagca ggcgtgagta agcattcagg
    20761 aatggtttat ccatcgtcac ggcctgtctg tctggcagga cacgatcggg atagtttttc
    20821 aacgttttga tatagctgaa gatgtaatcc cagcgaccgc agttcagacc aacaatatgg
    20881 tcgcgcagcg cgtgaaggat ttcgtccatc tggaacacgg cgggcagcgt ttcaatcagc
    20941 aacgtcgcct tgatggtgcc gcgcggcaga ttaaagcgat cttctgcata gctgaagact
    21001 tcgctccacc aggccgcttc ctgctaggac tgggttttcg gcagatagaa atagggaccg
    21061 ctgccctttg ccaacagtgc ctgatagttg tggaagaaat agagcgcaaa atcaaacagg
    21121 ctaccgggga ttgcctcacc acgccaggtg acatgttttt ccggcaagtg cagaccgcgt
    21181 acccgacaaa tcaaaaccgc tggattgggc ttgagctgat aaattttgcc tgcttcattg
    21241 gtatagctga tggtaccgtt aaccgcatcg cgcaggttaa tttgcccgtc gatcactttg
    21301 ttccagtctg gtgccagtga atcttcgaaa tcggccataa agactttcac attggcgttc
    21361 agtgcgttga tcaccatctt gcgctctacc gggccagtta tctctacgcg gcggtcttct
    21421 aagtccgcag gaatcccgcg aattttccaa tcagcatcgc gaatggaagc cgtttccgaa
    21481 ataaaatcag gcaacgttcc gttatcaata tcttgctgct gctgaatgcg cgctgccaga
    21541 agtttattgc gttgtggcgt aaaatgcgtc accagctcag tcagaaattc taccgcttcg
    21601 gcagtaagaa tttgcttctc ctgctcgcca tacggccttg tgaaagccag ttcatcggtt
    21661 gttgttgcct gttcagtcat cgtgcagctc ctcgtcatgg atccgaaaac ttcccctctg
    21721 tgaacgaagg aacgttgtgc gcttttcgtt cagcacaact aagactactc atttaaaatg
    21781 caaaatcaaa aacaatttcc attttaatta aaaatacttg ataacttatt gataacaata
    21841 tgaataaaat ttattcactt gctgaggtgc gtttcggaaa taaaaaaggc acccgaaggt
    21901 gcctgaggta aggtgctgaa tcgcttaacg atcgactatc acagaagatt aatccagcgt
    21961 tggattcatg tgccgtagat cgtatggcgt gatctgatag acgtaatagt tgagccagtt
    22021 ggtaaacagc aaattaccgt gactacgcca gctcgctcgc ggtttgtttt gcggatcgtt
    22081 gtgcgggaaa tagttatacg gtacatccgg gtctagtccg gcttccacat cgcgtaaata
    22141 ttcctgcgcc agcgtttgcg catcatattc gggatggccc gtcacaaagg caatgcgctt
    22201 atctttgctg gcaaacagat atgcatcccc ttcttccgtc tctgccagaa tttccagatc
    22261 ggtgtaatca cgaatcaacg ctgccggaaa gtcagcatag cgcgaatgcg gtgccaggaa
    22321 tgaatcatca aagccacgcg tcagaagcgc atgaggatgg agaatatgat gctcgtaaac
    22381 gccagagagt ttgtcggtgc gagtttgctt aggaatgccg tagaggatat tgagcgcggc
    22441 ctgtaccgcc cagcagacaa acagcgtcga ggtgacgtga tctttcgacc actccagcac
    22501 ctgtttgatc tgcggccagt aagcgacatc attaaactcc accaggccca gcggcgcacc
    22561 ggttacaatc agaccgtcaa agttctgttc ctgaatatct tcaaagttac agtagaagtt
    22621 gttcagatgc tctgcgggcg tgttgcgcga ttcacgggaa tcgatgcgca acagctgaat
    22681 atcgacctgc aaaggtgagt ttgaaagcag gcgcagaaac tgattttcag tttcaatctt
    22741 cttcggcatc aggttaagga tcagaacctt aagtggacga atttcctgac cagacgcacg
    22801 agaagttgtc atcacaaaga cgttttcttc acgcaagaaa ttgacggcgg gtagctcgtc
    22861 cggcacacga atcggcataa cctgattacc tcactacata cgcttatacg tttagacatc
    22921 cagatagctg aagataacca gaaaatttgc caatgtcgag cctgcatgtt gaaggtgaga
    22981 aagtttcaag gaaaaggcta cgttagaata taagaatgac gaaaaggaga gaaaatggtt
    23041 attagtattc gccgctcacg gcatgaggaa ggggaggaac tcgttgcgat ttggtgtcga
    23101 tctgtcgatg ccacccacga ttttttatca gcagagtatc gggccgagct ggaagagctg
    23161 gtgcgttcct ttctgccgga agcaccgttg tgggtcgcgg ttaatgaacg ggagcagccg
    23221 gttgggttta tgttgttaag tgggcagcat atggatgcgc tgtttatcga tcctgatgtg
    23281 tgcggctgcg gcgtgggtcg gatgctggtg gagcatgcgc tctcgatggc accggaactg
    23341 acaaccaacg ttaatgagca aaatgagcag gcggttgggt tctataagaa ggtgggtttt
    23401 aaggttacgg gacgctctga ggtggacgat ttggggaaac cgtatccgtt gctgaatctg
    23461 gcgtatgtgg gggaataagc cgcctctgcc gtgcctgccc aagctggaga agacg
//
LOCUS       DAJTRN010000062        22925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRN010000062 DAJTRN010000000
VERSION     DAJTRN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO328_002503"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO328_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5931030.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO328_002504"
     CDS             1207..1869
                     /locus_tag="OO328_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5931031.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO328_002505"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO328_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5931032.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO328_002506"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO328_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO328_002507"
     CDS             2795..3100
                     /locus_tag="OO328_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5931033.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO328_002508"
     CDS             3103..3507
                     /locus_tag="OO328_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5931034.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO328_002509"
     CDS             3497..3796
                     /locus_tag="OO328_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5931035.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO328_002510"
     CDS             3982..4278
                     /locus_tag="OO328_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5931036.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO328_002511"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO328_002511"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5931037.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO328_002512"
     CDS             5627..5995
                     /locus_tag="OO328_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5931038.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO328_002513"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO328_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5931039.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OO328_002514"
     CDS             7203..7904
                     /locus_tag="OO328_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5931040.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OO328_002515"
     CDS             7927..8091
                     /locus_tag="OO328_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931041.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OO328_002516"
     CDS             complement(8303..9613)
                     /locus_tag="OO328_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5931042.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OO328_002517"
     CDS             complement(9641..10972)
                     /locus_tag="OO328_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5931043.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO328_002518"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO328_002518"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5931044.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OO328_002519"
     CDS             complement(12683..13072)
                     /locus_tag="OO328_002519"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5931045.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO328_002520"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO328_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5931046.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO328_002521"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO328_002521"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5931047.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO328_002522"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO328_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5931048.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO328_002523"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO328_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5931049.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO328_002524"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO328_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5931050.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="OO328_002525"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="OO328_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5931051.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="OO328_002526"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="OO328_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="OO328_002527"
     CDS             21379..21726
                     /locus_tag="OO328_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931052.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="OO328_002528"
     CDS             21895..22545
                     /locus_tag="OO328_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931053.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6184 a         5781 c         5285 g         5675 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 gcggg
//
LOCUS       DAJTRN010000063        22409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRN010000063 DAJTRN010000000
VERSION     DAJTRN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            82..2682
                     /locus_tag="OO328_002529"
     CDS             82..2682
                     /locus_tag="OO328_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5931054.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="OO328_002530"
     CDS             2673..3656
                     /locus_tag="OO328_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5931055.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="OO328_002531"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="OO328_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="OO328_002532"
     CDS             4302..4817
                     /locus_tag="OO328_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5931056.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="OO328_002533"
     CDS             4783..5520
                     /locus_tag="OO328_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5931057.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="OO328_002534"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="OO328_002534"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5931058.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="OO328_002535"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="OO328_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5931059.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO328_002536"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO328_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5931060.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO328_002537"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO328_002537"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5931061.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="OO328_002538"
     CDS             10827..12734
                     /locus_tag="OO328_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5931062.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="OO328_002539"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="OO328_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5931063.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="OO328_002540"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="OO328_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5931064.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="OO328_002541"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="OO328_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5931065.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="OO328_002542"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="OO328_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5931066.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO328_002543"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO328_002543"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5931067.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="OO328_002544"
     CDS             complement(17402..19162)
                     /locus_tag="OO328_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5931068.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="OO328_002545"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="OO328_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5931069.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO328_002546"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO328_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5931070.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO328_002547"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO328_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5931071.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22409
                     /gene="sxy"
                     /locus_tag="OO328_002548"
     CDS             22005..>22409
                     /gene="sxy"
                     /locus_tag="OO328_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5931072.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGE"
BASE COUNT         5721 a         5258 c         5871 g         5559 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggag
//
LOCUS       DAJTRN010000064        22268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRN010000064 DAJTRN010000000
VERSION     DAJTRN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1015)
                     /locus_tag="OO328_002549"
     CDS             complement(<1..1015)
                     /locus_tag="OO328_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5931073.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAP"
     gene            complement(1117..2097)
                     /locus_tag="OO328_002550"
     CDS             complement(1117..2097)
                     /locus_tag="OO328_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5931074.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2105..2815)
                     /locus_tag="OO328_002551"
     CDS             complement(2105..2815)
                     /locus_tag="OO328_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5931075.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OO328_002552"
     CDS             complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OO328_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5931076.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5228..6637
                     /gene="glnA"
                     /locus_tag="OO328_002553"
     CDS             5228..6637
                     /gene="glnA"
                     /locus_tag="OO328_002553"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5931077.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6810..7859
                     /gene="glnL"
                     /locus_tag="OO328_002554"
     CDS             6810..7859
                     /gene="glnL"
                     /locus_tag="OO328_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5931078.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7871..9280
                     /gene="glnG"
                     /locus_tag="OO328_002555"
     CDS             7871..9280
                     /gene="glnG"
                     /locus_tag="OO328_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5931079.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9392..9502
                     /locus_tag="OO328_002556"
     CDS             9392..9502
                     /locus_tag="OO328_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5931080.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OO328_002557"
     CDS             complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OO328_002557"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5931081.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OO328_002558"
     CDS             complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OO328_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5931082.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12384..13016
                     /locus_tag="OO328_002559"
     CDS             12384..13016
                     /locus_tag="OO328_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5931083.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OO328_002560"
     CDS             complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OO328_002560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5931084.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16548..17480
                     /locus_tag="OO328_002561"
     CDS             16548..17480
                     /locus_tag="OO328_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5931085.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17521..18951)
                     /locus_tag="OO328_002562"
     CDS             complement(17521..18951)
                     /locus_tag="OO328_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5931086.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OO328_002563"
     CDS             complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OO328_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5931087.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OO328_002564"
     CDS             complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OO328_002564"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5931088.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20812..21081)
                     /locus_tag="OO328_002565"
     CDS             complement(20812..21081)
                     /locus_tag="OO328_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5931089.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21151..21735
                     /gene="mobA"
                     /locus_tag="OO328_002566"
     CDS             21151..21735
                     /gene="mobA"
                     /locus_tag="OO328_002566"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5931090.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21717..22244
                     /gene="mobB"
                     /locus_tag="OO328_002567"
     CDS             21717..22244
                     /gene="mobB"
                     /locus_tag="OO328_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5931091.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5514 a         5579 c         5330 g         5845 t
ORIGIN      
        1 taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg
       61 ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt acgagcgcag
      121 cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg
      181 aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt
      241 aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg
      301 tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc cagatggttt
      361 tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct tttttcacct
      421 caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg cctacggcaa
      481 tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca ctgccaaacc
      541 aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt
      601 cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt accgacttga
      661 gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga
      721 tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg
      781 agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac agaaaaaagc
      841 ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat
      901 ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg
      961 taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg agcataacat
     1021 cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac cattcatatg
     1081 gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa gatggtttgc
     1141 ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa ttgcatcttc
     1201 tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg tttcactcat
     1261 ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg ccgacgcata
     1321 gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat cgccaaggca
     1381 gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa tacaggcttt
     1441 atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga cctgggtaat
     1501 atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga tcatgttagc
     1561 aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg acaccagctc
     1621 atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt cctgatacgt
     1681 ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca aattaccttc
     1741 ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg cgacggcttc
     1801 gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt tcagacggcg
     1861 ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc
     1921 gccatccaga acatggattt tgacgccacg tagattattt tcactggcga tatcaagtaa
     1981 atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag catgtaaata
     2041 aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg tcaccataaa
     2101 acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct
     2161 ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg
     2221 cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt
     2281 tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt
     2341 caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa
     2401 ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt gatggcgtca
     2461 ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc tcggtaaacc
     2521 cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg
     2581 gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta cggaagattt
     2641 tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta atgcccagca
     2701 gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg ccatctttca
     2761 accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc gccattgttt
     2821 aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa gaaagcaatt
     2881 aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta atcacagact
     2941 tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa cctgccatcg
     3001 ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg cggttggcgc
     3061 ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggagtcactt
     3121 ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg
     3181 gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg
     3241 tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc tgaccttcgt
     3301 aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg aacagcgcga
     3361 acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc tgacccactt
     3421 cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca gtaccggaag
     3481 tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc acgtagtcga
     3541 gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca
     3601 gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg ttttcatacg
     3661 gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat accgccagtt
     3721 cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca ccacgaccag
     3781 aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca
     3841 gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct tctttaccgc
     3901 agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg
     3961 ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccca
     4021 ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata cgttccagac
     4081 cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg ctatcgatga
     4141 tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg ccgataacgc
     4201 caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa
     4261 cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg tcttccgcca
     4321 tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt
     4381 ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc
     4441 aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt
     4501 aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa
     4561 ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg
     4621 cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt
     4681 tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt
     4741 gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg
     4801 ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt
     4861 tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg
     4921 atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc tttcacggag
     4981 cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt tccattgaag
     5041 cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg ttaagcatga
     5101 taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct tttttacggc
     5161 gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa tccaggagag
     5221 ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt
     5281 tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc ctgctcatca
     5341 ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg
     5401 gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg cagtgattga
     5461 cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac ctggcaccct
     5521 gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt acctgcgttc
     5581 cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc tgttcgatga
     5641 catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata tcgaaggcgc
     5701 atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg cagtgaaagg
     5761 cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg aaatgtgtct
     5821 ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag cgactgctgg
     5881 tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat
     5941 ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga cctttatgcc
     6001 aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc tgtctaaaaa
     6061 cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg cgctgtacta
     6121 cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc cgaccaccaa
     6181 ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt actctgcgcg
     6241 taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga
     6301 agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc tgctgatggc
     6361 cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca aaaacctgta
     6421 tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc
     6481 actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt tcacggatga
     6541 agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc
     6601 gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc
     6661 gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
     6721 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
     6781 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
     6841 agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg gcgatccatt
     6901 acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacgc
     6961 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
     7021 aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt cgctcgcata
     7081 tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag atggcaccga
     7141 tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa gtagctgccc
     7201 gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg
     7261 cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat accaaagtga
     7321 ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg ccgcagctgc
     7381 ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta acgctggtgt
     7441 cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc ctgccggaac
     7501 tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc aatgcgctac
     7561 aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg tttcaactga
     7621 ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat aacgggccgg
     7681 gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg
     7741 gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca ggcaaaattg
     7801 aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc aggaaataaa
     7861 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
     7921 ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg agaacggcgc
     7981 ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
     8041 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc caatgcttcc
     8101 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
     8161 ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc tggttgagcg
     8221 cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta acggcccaac
     8281 gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta tcggtcggct
     8341 ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt
     8401 cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg cgctgaatat
     8461 ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga aaggcgcgtt
     8521 tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg gcacgttatt
     8581 tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
     8641 agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
     8701 cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct
     8761 gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac gtcgggaaga
     8821 tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg gcgtagaagc
     8881 gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc caggcaacgt
     8941 gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc aggaagtgtt
     9001 gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat
     9061 gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc tgcgttccgg
     9121 tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac tgacgaccgc
     9181 gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct ggggccgcaa
     9241 cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt gtgtgtaaag
     9301 attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat aagttcagta
     9361 tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc
     9421 gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc
     9481 gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga aagtcgtagg
     9541 cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg atgcggcgta
     9601 aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc cagcgcatca
     9661 ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta aacgaagcgc
     9721 cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg cgcagatagg
     9781 tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc acctgtattc
     9841 ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc ttgagatctt
     9901 ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg taatccagac
     9961 ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca cgcgttagcg
    10021 caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac tgcttcaact
    10081 ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg cccatcccca
    10141 gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc acgccttcac
    10201 gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg ataaactgat
    10261 agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt tgctgcggac
    10321 tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc agatgcgcgt
    10381 agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg gtaaaggcga
    10441 aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg gaggtaaaac
    10501 caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc tcgcggttaa
    10561 ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga ttaaagcctt
    10621 cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact tcgatcgaaa
    10681 tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga cggctgattt
    10741 gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg acgtgacgcc
    10801 cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga tactgatcgg
    10861 ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa agcttatggc
    10921 agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc acggcttgta
    10981 aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac gaggtgtatc
    11041 gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt tgctgtacag
    11101 acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg gatgcccgtt
    11161 tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct gtagtttaac
    11221 gaataaccac accagataac atataaccgg aagggttatc agcagaacgg gaaggcccac
    11281 ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct tctcgtcttc
    11341 ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact catcaatacg
    11401 atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc ctgcttccag
    11461 acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact ccgcctgcgg
    11521 tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt cagtcactcc
    11581 caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct ggcctttgct
    11641 gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac gacgtttttt
    11701 ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc gacgcgcttt
    11761 tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc gtcttttagc
    11821 cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa gaaaaaaggc
    11881 gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc ttacaatcct
    11941 tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt tgattccatt
    12001 tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct tcgcctcctg
    12061 gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc tatgggtgct
    12121 actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca cattttcaga
    12181 gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat cgcagaattt
    12241 tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg gcttttactt
    12301 acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc gccacaaagc
    12361 tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg cattttgtga
    12421 tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg gcttttgcag
    12481 gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag aaaagcctgg
    12541 ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa gtggctgacg
    12601 gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa gagatgaagc
    12661 gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg caaggtctgg
    12721 tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg attgagtggg
    12781 cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa ctggcaagcg
    12841 gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt aacggtgatg
    12901 tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg aggcagaaac
    12961 tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc gaataatttt
    13021 cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa tagattacaa
    13081 ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt ctttcgccca
    13141 ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt
    13201 acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact
    13261 ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag ttgttgcata
    13321 ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg
    13381 gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc
    13441 agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg
    13501 gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata
    13561 cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc ccgtttgata
    13621 atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga
    13681 cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca
    13741 acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg
    13801 tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc
    13861 cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta
    13921 cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc cgccgttgcc
    13981 cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg cgagagatgc
    14041 gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata atcctctggc
    14101 gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg
    14161 ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc
    14221 acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt cgacttcagc
    14281 ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct
    14341 tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaata
    14401 ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt aaattcttca
    14461 cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag ctcttcagaa
    14521 tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcacc
    14581 ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg tttctgcaga
    14641 tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc ggcgtaacgt
    14701 ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt acccgcaatc
    14761 tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga
    14821 cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc
    14881 agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg cccgaccttc
    14941 agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc gtgagagatt
    15001 tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac gcctggctcg
    15061 atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta
    15121 tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca
    15181 tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg
    15241 tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc ctgtaaccat
    15301 ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa cagccccaac
    15361 aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc cagttcaaca
    15421 tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt ttgctcgagc
    15481 ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca
    15541 tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt ttcaccgacg
    15601 cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa gtcgatgatc
    15661 aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat ggtattcgtc
    15721 atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgccagtg
    15781 ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc gataacgtcg
    15841 tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc
    15901 agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga tttgtaatgt
    15961 tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc
    16021 ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat cgcaccggtc
    16081 ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat
    16141 gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt
    16201 tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt ttcaccgctt
    16261 ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt ggataacttt
    16321 gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag catgatgttt
    16381 gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga taatgggatt
    16441 ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg ttatttgggt
    16501 cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg gctaaccttt
    16561 tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat
    16621 tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg attgtaaaac
    16681 tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt
    16741 attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg caatgggaag
    16801 ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta
    16861 gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat
    16921 atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc tgggcgctgg
    16981 atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg gagcgacgtg
    17041 gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca
    17101 ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acccactcca
    17161 cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg
    17221 gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc
    17281 cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg gatttacagc
    17341 ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc
    17401 agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct ctcatgtcat
    17461 cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg agtgagtatt
    17521 ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt
    17581 aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt tgtcaatcgt
    17641 gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat cagcctgctt
    17701 ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac
    17761 gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt ttgtggatga
    17821 tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc
    17881 gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata
    17941 ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg
    18001 gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt ggcgcactgc
    18061 tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc
    18121 ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac
    18181 gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt
    18241 gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt tcttttcttc
    18301 taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca ttggtgataa
    18361 ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg aactaccatc
    18421 accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg caggggcaac
    18481 atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga
    18541 tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta agttcacttt
    18601 tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg gcaacggacc
    18661 gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga gatcgggtgc
    18721 accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt tgtcaaagtt
    18781 tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat cgttaaaatc
    18841 tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc
    18901 ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca tataaatatt
    18961 ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt ccagcggcag
    19021 gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg tgaatattca
    19081 cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc accgtatcag
    19141 catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga ttcagctgat
    19201 atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct gcggcttttt
    19261 cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg tactcttcac
    19321 ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga atggtctggg
    19381 ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc agcgccatcg
    19441 ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag ttgacgtggt
    19501 atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa atatgcagaa
    19561 cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc acttgcggcg
    19621 cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac tgcgccgccg
    19681 atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc atgatctact
    19741 ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac ttttgcctgt
    19801 tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca cgggaaattt
    19861 ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata aaccaaacgc
    19921 atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct aaattcttca
    19981 taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc attgagcaac
    20041 atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac aaacattgga
    20101 ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag aacggtgaaa
    20161 tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt cagaaatgcc
    20221 gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg ctcaatgaga
    20281 tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc cgtctggtgc
    20341 atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc ttcgaactgg
    20401 cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa taaagtctga
    20461 ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg caacgcaaat
    20521 tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa tttgacgaca
    20581 aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct gttaagcggg
    20641 gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat ggtatccggg
    20701 tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa acattacgaa
    20761 tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct tttactcttt
    20821 aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat ctttctgcaa
    20881 acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga tcagaatgtc
    20941 atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt tctgcaaaaa
    21001 ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt caataacttc
    21061 attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca cgttagcagg
    21121 gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat aacaggcgtt
    21181 gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt gctggactta
    21241 aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct ctctcacgtc
    21301 gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa agtgattgaa
    21361 gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat gcagcaggaa
    21421 gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca tgatttagcc
    21481 gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga cggtgaacgc
    21541 gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact ggaatatctg
    21601 caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca tgcggttgat
    21661 ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct tgcccgatgg
    21721 caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc ggaaaaacta
    21781 cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg gggctgatta
    21841 agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat gagctgcgca
    21901 aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg atgacggaaa
    21961 caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat acctcaaagc
    22021 tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt gtgctgtttc
    22081 gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt attgctgtag
    22141 ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat gttgaggggc
    22201 tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga gtaggctgga
    22261 tcagacgc
//
LOCUS       DAJTRN010000065        22189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRN010000065 DAJTRN010000000
VERSION     DAJTRN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..434)
                     /locus_tag="OO328_002568"
     CDS             complement(<1..434)
                     /locus_tag="OO328_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5931092.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTG"
     gene            442..942
                     /gene="tnpA"
                     /locus_tag="OO328_002569"
     CDS             442..942
                     /gene="tnpA"
                     /locus_tag="OO328_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5931093.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(981..1328)
                     /gene="grxD"
                     /locus_tag="OO328_002570"
     CDS             complement(981..1328)
                     /gene="grxD"
                     /locus_tag="OO328_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5931094.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1663..2490
                     /gene="mepH"
                     /locus_tag="OO328_002571"
     CDS             1663..2490
                     /gene="mepH"
                     /locus_tag="OO328_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5931095.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2618..3199
                     /gene="sodB"
                     /locus_tag="OO328_002572"
     CDS             2618..3199
                     /gene="sodB"
                     /locus_tag="OO328_002572"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5931096.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3345..4514)
                     /locus_tag="OO328_002573"
     CDS             complement(3345..4514)
                     /locus_tag="OO328_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5931097.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="OO328_002574"
     CDS             complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="OO328_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5931098.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            5068..6093
                     /gene="purR"
                     /locus_tag="OO328_002575"
     CDS             5068..6093
                     /gene="purR"
                     /locus_tag="OO328_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5931099.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6090..7022)
                     /locus_tag="OO328_002576"
     CDS             complement(6090..7022)
                     /locus_tag="OO328_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5931100.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            7135..8346
                     /locus_tag="OO328_002577"
     CDS             7135..8346
                     /locus_tag="OO328_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5931101.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8637..9785
                     /gene="cfa"
                     /locus_tag="OO328_002578"
     CDS             8637..9785
                     /gene="cfa"
                     /locus_tag="OO328_002578"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5931102.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="OO328_002579"
     CDS             complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="OO328_002579"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5931103.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10681..12054
                     /gene="mdtK"
                     /locus_tag="OO328_002580"
     CDS             10681..12054
                     /gene="mdtK"
                     /locus_tag="OO328_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5931104.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12095..13351)
                     /locus_tag="OO328_002581"
     CDS             complement(12095..13351)
                     /locus_tag="OO328_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5931105.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13659..13735
                     /locus_tag="OO328_002582"
     tRNA            13659..13735
                     /locus_tag="OO328_002582"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13693..13695,aa:Val,seq:gac)
     gene            13740..13816
                     /locus_tag="OO328_002583"
     tRNA            13740..13816
                     /locus_tag="OO328_002583"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13774..13776,aa:Val,seq:gac)
     gene            13924..14229
                     /locus_tag="OO328_002584"
     CDS             13924..14229
                     /locus_tag="OO328_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5931106.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14355..15777
                     /locus_tag="OO328_002585"
                     /pseudo
     CDS             14355..15777
                     /locus_tag="OO328_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15789..16601)
                     /locus_tag="OO328_002586"
     CDS             complement(15789..16601)
                     /locus_tag="OO328_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931107.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16605..17390)
                     /locus_tag="OO328_002587"
     CDS             complement(16605..17390)
                     /locus_tag="OO328_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5931108.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17387..18055)
                     /locus_tag="OO328_002588"
     CDS             complement(17387..18055)
                     /locus_tag="OO328_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5931109.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(18119..18757)
                     /locus_tag="OO328_002589"
     CDS             complement(18119..18757)
                     /locus_tag="OO328_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5931110.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18770..20849)
                     /locus_tag="OO328_002590"
                     /pseudo
     CDS             complement(18770..20849)
                     /locus_tag="OO328_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20870..21496)
                     /locus_tag="OO328_002591"
     CDS             complement(20870..21496)
                     /locus_tag="OO328_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5931111.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21952..22161)
                     /gene="fumD"
                     /locus_tag="OO328_002592"
     CDS             complement(21952..22161)
                     /gene="fumD"
                     /locus_tag="OO328_002592"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5931112.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5541 a         5746 c         5157 g         5745 t
ORIGIN      
        1 cccgtgacct gacggctatt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgtcctgc gggtagcgga atgcagcatt ctgtccccgc
      121 tttttgaatc ggggaaaagc agcccgcttc tggaagaagt ttttgtaagc ccgctcaagg
      181 tctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg
      241 gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg
      301 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc
      421 tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg
      481 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac caaataccga
      541 cgccagattt ttgaccatga cacgacagaa aaactacgca cttacttttc aaatgtatgt
      601 gctgattttg cagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta
      661 attaactatc ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcatatcg
      721 aataggttac tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt
      781 ttgtggagtc ctggctattt tgccagtagc tgcgaaggtg cgccaatatc cgtcatccgc
      841 cagtacattg aacaacagca aacacctggt caggtggaaa accgcgcctt atatccccgc
      901 cctgaaggac gaggttttac ggcgcaccgg ataaaggact gacggatgcc cgttcgcatc
      961 cgtcagtatt gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca
     1021 gtttctttga tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa
     1081 ccgccgacca gctcgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc
     1141 ggcagttccg cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca
     1201 catgcggcaa gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa
     1261 cctttcatgt acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg
     1321 gtgctcattg tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt
     1381 cagtgcggca tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct
     1441 atttgttgca tttcaaatat tcgtttcttc tctgatgcgc ggcaaactgg cgtatttata
     1501 accattttta gctttcactg ctgcattttt ttgcgctcgc caacgaaacg tattttttaa
     1561 caataaaagc tattaacttt ctcttcttct atgcattaga atcatcaagt tttgtaaatc
     1621 agacgcaggc atgatagacc tgccttaaca gagggacgct cagtggcgcg gataaaccgt
     1681 atttcgatca cgctctgtgc tttacttttt accaccctgc ctttaacgcc tatggcccat
     1741 gcttcaaagc aagccaggga gagttctgct accactcata tcaccaaaaa agcagataaa
     1801 aagaaaagca cggcaaccac caaaaagacc cagaaagcca gccagaaaac agcgaaaaaa
     1861 gccgccagta aaagtatgac caaaagcaaa accgcttctt ctgttaaaaa atcttccatt
     1921 accgcttcta aaaacgccaa aactcgcagc aaacacgccg tcaataaaac ggcctcagcc
     1981 agctttaccg aaaagtgtac caaacgtaag ggctataaat cgcattgtgt gaaagtcaaa
     2041 aatgccgcgt caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa agccacgaaa
     2101 gtggcaatga ataaactgat gcagcaaatt ggtaagccat atcgttgggg cggcagctca
     2161 ccgcgtaccg gttttgattg cagcggcctg gtttattacg cttataaaga tttggtgaaa
     2221 attcgtattc cgcgtacggc gaatgaaatg tatcacctgc gtgatgcagg acctatcgaa
     2281 cgtagtgagc tgaaaaacgg cgacctggtc tttttccgta ctcagggacg cggtacagcc
     2341 gatcatgtcg gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg cacaggtcag
     2401 gaaattcaaa tcacttctct cagtgaagac tactggcagc gccactatgt tggcgctcgt
     2461 cgggtaatga ccccaaaaac acttcgctaa aactttaccc tgttgttacg gcaacagggt
     2521 aagttcatct tttgtctcac cttttaattt gctaccctat ccatacgcac aataaggcta
     2581 ttgtacgtat gcaaattaat aataaaggag agtagcaatg tcattcgaat tacctgcact
     2641 accatatgct aaagatgctc tggcaccgca catttctgcg gaaactatcg agtatcacta
     2701 cggcaagcat catcagactt atgtcactaa cctgaacaac ctgattaaag gtaccgcgtt
     2761 tgaaggtaaa tcactggaag agattattcg cagctctgaa ggtggcgtat tcaacaacgc
     2821 agctcaggtc tggaaccata ctttctactg gaactgcctg gcaccgaacg ccggtggcga
     2881 accgactgga aaagtcgctg aagctatcgc cgcatctttt ggcagctttg ccgatttcaa
     2941 agcgcagttt actgatgcag cgatcaaaaa ctttggttct ggctggacct ggctggtgaa
     3001 aaacagcgat ggcaaactgg ctatcgtttc aacctctaac gcgggtactc cgctgaccac
     3061 cgatgcgact ccgctgctga ccgttgatgt ctgggaacac gcttattaca tcgactatcg
     3121 caatgcacgt cctggctatc tggagcactt ctgggcgctg gtgaactggg aattcgtagc
     3181 gaaaaatctc gctgcataat aactgatggc aaatgcagca ttgcctgatg cgcttcgctt
     3241 accatgccaa cgcggatgag gcgttaacag cgcattcggc aagataaatc gcacgttgtc
     3301 agcaactgta acgcagaagg ttatccttct gcgtttttgt ttaattagct gttagcaacg
     3361 caaactgttt caggttgttt tctggctgac ataaacacca gcaataatgc cagtcccgcg
     3421 acaatcgccc ccgtcaccgg cacaaagctg tatcccagcc cagcggaaat taccgcacca
     3481 ccagcagctg ctcccagcgc atttccaaga ttaaaggcac caatattgac tgatgaagac
     3541 agacctggcg cttcactggc gacatgcatc acgcgcatct gtaacggagg tacaaccgca
     3601 aaggttgctg cgccccacac caccatgcta atagctgcgc cgaactcatt gcgggccagg
     3661 aacgggattg ccagcataat caccatcagc agcaacaaaa agcctttcaa cgtgccgtta
     3721 actgaacgat ctgccagttt gccgccgaga tagttaccga tagagaatcc gacaccaatc
     3781 agcaccagca ttgccgtgac gaacaccggt gttgcgtggg taatactttg cagtaccgga
     3841 gagatatagg tgtagagggt aaacattgca ccagctccca gtaccgtcgt cagcaatgca
     3901 gacagcacct gcggacgcat taataccgcc agctcttttt tcacttcagg tcgtgcccct
     3961 gtaccacctt taggtaatga gaagaacaga cttaccattg aaatcactcc cagccccgcc
     4021 gttgccagaa atgacatccg ccagccgatg gtttcaccca accaggtcgc cgccggcacg
     4081 ccaccgatat ttgccagggt taaccccata aacatagtgg caactgcgct ggcctgttta
     4141 tgttttggca ccacgcttgc ggccacgact gaacccaaac caaaaaatgc tccgtgattc
     4201 aggctggtca aaatacgtga aagcatcagg gtcatataat ccggcgcgat ggcggaaaga
     4261 acgttgccta gcgtgaaaat tgccatcagg aaaatcaacg cactgcggcg ggcacgatga
     4321 gaaagtagaa gcgtcatcag cggcgcgccc accattacgc caactgcata ggcactgatt
     4381 aacattccgg cagcgggaat cgagacatcc acaccgcgcg caatgacggg caacaagccc
     4441 attggcgaga actccgttgt cccgatacca aacgcgccaa tcgccagcgc cagcaacgga
     4501 tagttaattt tcatgcctta tctccacctc ttcgcgtcat tacgcgatat tcattaaagt
     4561 ggcgaaagca tgacagcaat cacaaaaaaa tgaaaataac aaaaagagaa aacacttttg
     4621 ccattttgct aacaaacagg aaggagatgc gagggagaac gcgctccctc gagaggaaat
     4681 cagtgcagcg cggcagtcaa acccacggct acgatcaaac cgaggacgat aaccgttgtt
     4741 accagtgaaa atttaaggtc ggtgctcatc aagttttctc cttttttatt accacacaaa
     4801 aagtgatatt acgcattttt acacactgtg atgaaaaaat ctcccgtcat ttataatgat
     4861 aagtgttttt accacttccc cttttcgtca agatcggcca aaattccacg cttacactat
     4921 ttgcgtactg gccattgacc ccttcctgac gctccgtgtc gtttttccgg cgtaccgcaa
     4981 cacttttgtt gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgatt ttgcaggagc
     5041 tgaagttagg gtctggagtg aaatggaatg gcaacaataa aagatgtagc gaaacgagca
     5101 aacgtttcca ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt cgctgaagaa
     5161 acgcgcaacg ccgtgtgggc agcgattaaa gaattacact actcccctag cgcggtggcg
     5221 cgtagcctga aggttaacca caccaagtct atcggtttgc tggcgaccag cagcgaagcg
     5281 gcctattttg ccgagatcat tgaagcagtt gaaaaaaatt gcttccagaa aggttacacc
     5341 ctgattctgg gtaatgcgtg gaacaatctt gagaaacagc gggcttatct gtcgatgatg
     5401 gcgcaaaaac gcgtcgatgg tctgctggtg atgtgttctg agtacccaga gccgttgctg
     5461 gcgatgctgg aagagtatcg ccatatccca atggtggtga tggactgggg tgaagcaaaa
     5521 gctgacttca ccgatgcggt cattgataac gcgttcgaag gcggctacat ggccgggcgt
     5581 tatctgattg aacgcggtca ccgcgaaatc ggcgtcatcc ccggcccgct ggaacgtaac
     5641 accggcgcag gccgccttgc cggttttatg aaggcgatgg aagaagcgat gatcaaggtg
     5701 ccggaaagct ggattgtgca gggtgacttt gaacctgaat ccggttatcg cgccatgcag
     5761 caaatcctgt cgcagccgca tcgccctact gccgtcttct gtggtggcga tatcatggca
     5821 atgggcgcac tttgtgctgc tgatgaaatg ggcctgcgcg tcccgcagga tgtttcgctg
     5881 atcggttatg ataacgtgcg caacgcgcgc tattttacgc cggcgctgac cacgatccat
     5941 cagccaaaag attcgctggg tgaaacagcg ttcaacatgc tgttggatcg tatcgtcaac
     6001 aaacgtgaag aaccgcagtc cattgaagtg catccgcgtt tgattgaacg ccgctccgtg
     6061 gctgacggcc cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt ctcttccggc
     6121 tcccgcagcc actccttatt cagcgtctca ctatcgctga gatactcaag cagccaggtt
     6181 aacgcaggcg acatatcatt ttgctgccat gtcagacaac atgccgaatc cgggaagggg
     6241 ttttccagtt caagtgctac ccacttcccc tcattaagcc acggtttggc gaaatgtgtt
     6301 ggcaccatcc ctatgcataa tcctgccgag atacaggttg ccgatgattc ccagtcaggt
     6361 acgacaagtc ttttttggtt atccagcaac caggtaatac gtttaggtag cgttcgcgag
     6421 gtgtcttcgc gcaccaacga cggccagttg cgcaacgtat catcgctgaa cgggccatcc
     6481 atcaacgcca gcgggtggtg actggcaaca acgcaactcc agcttagcat ccccatatcc
     6541 cggaaggcat aacgaccgcc taccggaatc gcgcgtgttg cgccaattgc cagttccacg
     6601 cgcccgtcgg aaagcgcatc ccagacaccg ttgaacactt cctgaaagac aagaagttcg
     6661 acatcatcaa aatggcgata aaaatcaacg atcatctgcc gtgtacgttc tggcctgaca
     6721 atattatcca ctgcgatagc taactgaccg cgccagccgt tcgctatctg ctgacattgc
     6781 tggcgggtga tctgcatttt tttgacaaca gagcgccctt ctttgagaaa ccacgctcca
     6841 gcagcggtca gctccacatc acggtgccgt cgttcaaaga gcggtaccgc cagccactct
     6901 tccagctgac gcacggtata gctgaccgca gaaggaacac gatgcagctc ctgtgccgca
     6961 gcgctaaaac taccattacg cgctaccgca tcaacaactt cgagtgaata ttctgaccac
     7021 atagtctgcc tgcaaaattt ttgaaaccag tcatcaaata ttaccgtttc acaacactaa
     7081 tttcactccc tacactttgc ggcggtgttt aattgagaga tttagagaat atacatgcaa
     7141 cctgggaaaa gatttttagt ctggctggcg ggtttgagcg tactcggttt tctggcaacc
     7201 gatatgtatc tgcctgcttt cgccgccata caggccgacc tgcaaacgcc tgcgtctgct
     7261 gtcagtgcca gccttagtct gttccttgcc ggttttgccg cagcccagct tctgtggggg
     7321 ccactctccg accgttatgg tcgtaaaccg gtgttattaa tcggcctgac aatttttgcg
     7381 ttaggtagtc tggggatgct gtgggtagaa aacgccgcca cgctgctggt attgcgtttt
     7441 gtacaggctg tgggggtctg cgccgcggcg gttatctggc aagcattagt gacagattat
     7501 tatccttcac agaaagtcaa ccgtattttt gcgaccatca tgccgctggt gggtctgtct
     7561 ccggcactgg ctcccctgtt aggaagctgg ctgctggtcc atttttcctg gcaggcgatt
     7621 ttcgccaccc tgtttgccat taccgtggtg ctgattctgc ctattttctg gctcaaaccc
     7681 acgacgaagg cccgtaacaa tagtcaggat ggtctgacct ttaccgacct gctacgttct
     7741 aaaacctatc gcggcaacgt gctgatatac gcagcctgtt cagccagttt ttttgcatgg
     7801 ctgaccggtt caccgttcat ccttagtgaa atgggctaca gcccggcagt tattggttta
     7861 agttatgtcc cgcaaactat cgcgtttctg attggtggtt atggctgtcg cgccgcactg
     7921 cagaaatggc aaggcaagca gttattaccg tggttgctgg tgctgtttgc tgtcagcgtc
     7981 attgcgacct gggctgcggg cttcattagc catgtgtcgc tggtcgaaat cctgatccca
     8041 ttctgtgtga tggcgattgc caatggcgcg atctacccta ttgttgtcgc ccaggcgctg
     8101 cgtcccttcc cacatgcaac cggtcgcgcc gcagcgttgc agaacactct gcaactgggt
     8161 ctgtgcttcc tcgcaagtct ggtagtttcc tggcttatta gtatcagcac gccattgctc
     8221 accaccacca gcgtgatgtt atcaacagta gtgctggtcg cgctgggtta catgatgcaa
     8281 cgttgtgaag aagttgactg cccgaatcat ggcaatgccg aagtcgctca tagcgaatca
     8341 cactgatcta tatcgatata cttatactta ggctgctaac aaaattttgt tgtatgattg
     8401 aaattagcgg cctatactaa tttcgagttg ttaaagctac gataaatatt atgtttttac
     8461 ggggacagga tcgttcccga ctcactatgg atggtcattt cggcaagggt tcctcctttc
     8521 cctctgttct acgtcggatt atagactcgc ggttttttct gcgagatttc tcacaaagcc
     8581 caaaaagcgt ctacgctgtt ttaaggttct gatcaccgac cagtgatgga gaaactatga
     8641 gttcatcgtg tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt atcgccaacg
     8701 aattacttag ccgtgccggt atagccatta acggttctgc cccggcggat attcgtgtga
     8761 aaaaccccga tttttttaaa cgcgttctgc aggaaggctc tttggggtta ggcgaaagtt
     8821 atatggatgg ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa gtcttacgcg
     8881 caggtctcga gaaccaactc ccccatcatt tcaaagacac gctgcgtatt gccggcgctc
     8941 gtctcttcaa tctgcagagt aaaaaacgtg cctggatagt cggcaaggag cattacgatt
     9001 tgggtaatga cttgttcagc cgcatgcttg atcccttcat gcaatattcc tgcgcttact
     9061 ggaaagatgc cgataatctg gaatctgccc agcaggcgaa gctcaaaatg atttgtgaaa
     9121 aattgcagtt aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg ggcggactag
     9181 cgcactacat ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt tctgccgaac
     9241 agcaaaaaat ggctcaggaa cgctgtaaag gcctggatgt caccattttg ctgcaagatt
     9301 atcgtgacct gaacgaccag tttgatcgta ttgtttctgt ggggatgttc gagcacgtcg
     9361 gaccgaaaaa ttacgatacc tattttgcgg tggtggatcg taatttgaaa ccggaaggta
     9421 tattcctgct ccatactatc ggttcgaaaa aaaccgatct gaatgttgat ccctggatta
     9481 ataaatatat ttttccgaac ggttgcctgc cctctgtacg ccagattgct caatccagcg
     9541 aaccccactt tgtgatggaa gactggcata acttcggtgc tgattacgat actacgttga
     9601 tggcgtggta tgaacgattc ctcgccgcat ggccagaaat tgcggataac tacagtgaac
     9661 gctttaaacg gatgtttacc tattatctga atgcctgcgc aggtgctttc cgcgcccgtg
     9721 atattcagct ctggcaggtg gtgttctcac gcggtgttga aaacggcctt cgagtcgctc
     9781 gctaaaggct attctatcgc cccctcttcg ggggcgagtt cagatcaggc ttctgtacct
     9841 ggttgattca tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc taccactgcc
     9901 tgagtttgtg gatcgatttc aatgttgacg cgtgcgccaa gttttttctt cccaagagtc
     9961 gtgcgttcca gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt gacttcgccg
    10021 acggtcaggc taataccgtc gatgccaata aatcctttgt acaggatata tttcatcaac
    10081 tgactatcct ggactttaaa ccagatctgg cgattatttt ccgaggttaa tattttcgcc
    10141 acttcagcgg tggtcataat atgacctgac attaagtgtc cgccaatttc atcactgaat
    10201 ttcgccgcac gctcaacgtt tacccaatcc cccactttta aatcgccaag attggtaatg
    10261 cgtaacgttt ctttcatcag gtcaaaactg acatggttgc cgttaatttc cgtcacggtc
    10321 aggcagcaac cgttatgcgc cacggaagca ccggtttcca ggccgtccag catgtggtcg
    10381 ggtaactcca ccacatgcgt acgaaaattt ggtttctcgt caatcgacac cagttttgcg
    10441 gtgccctgta caatccccgt aaacatactt acaactcctg aaatcagtta agacattctg
    10501 ttcagcacaa tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa tggctatttt
    10561 ttcactggag aattaataaa tcctcgctac aatagactga atttcccctg cttcttcttt
    10621 ttgctgccca ttcaggcggc tttttagtct ctcatataac tacaaataaa aggtgttcac
    10681 gtgcagaagt atatcagtga agcgcgtctg ttattagcat tagcaatccc ggtgattctc
    10741 gcgcaaatcg cccaaactgc gatgggtttt gtcgataccg tgatggcggg cggctatagt
    10801 gccaccgaca tggcggcggt cgctatcggt acttctatct ggcttccggc gatcctcttt
    10861 ggtcacggac tgctgctggc attaacgccg gttatcgcgc aattaaatgg ttccggtcga
    10921 cgtgagcgca ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt tgtttccgtt
    10981 ctcattatgc tggtgctgtg gaatgcaggt tacattatcc gctacatgga aaacatcgat
    11041 ccggctctgg cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg cgcgccggga
    11101 tatctgttct tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac caagccgggt
    11161 atggtaatgg gctttatcgg cctgctggtg aacatcccgg tgaactatat ctttatttat
    11221 ggtcatttcg gtatgcctga gctcggtggc gttggttgtg gcgtggctac tgcggcggtg
    11281 tattgggtca tgttccttgc catggtttct tacattaaac gcgcccgctc catgcgcgat
    11341 attcgtaacg aaaaaggcac cgctaagccc gagcctgcgg ttatgaaacg actgattcaa
    11401 ctcggtttgc cgattgcgct ggcactgttc tttgaagtga cactgtttgc cgtcgtggct
    11461 ctgttagtgt ctccgctcgg tattgttgat gtcgcaggac accagattgc cctgaacttt
    11521 agttcactga tgtttgtgct tccaatgtcg ctggcggcag cggtaactat ccgcgtaggt
    11581 tatcgtctgg gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac cgggcttatg
    11641 gtgggtgtct gtatggcaac cctgacggcc attttcacgg tttcactgcg ggagcaaatc
    11701 gccctgttgt acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt gatgttgctg
    11761 gcggcggtat atcagatttc tgactcaatc caggtgattg gcagtgggat tttgcgtggt
    11821 tataaagata cgcgttccat tttctatatc acctttacag cttactgggt gctgggcttg
    11881 ccaagcggct atattctggc actgaccgat ctggtcgttg aacctatggg gccagcaggc
    11941 ttctggatag gctttattat cggcctgaca tcggcagcca ttatgatgat gctgcgtatg
    12001 cggttcctgc aacgtatgcc gtcagccatc attctgcaac gagcgtcccg ctaataaaga
    12061 caaggcgcaa ccttcacggg ttgcgcctgt gtttttacgc aggctggagc gttgcgccaa
    12121 tcccgtcttc gtctggctgt aatttcagag cgttacacag agtttgccga ctggcatctg
    12181 ttatctcaac gcgtatatcc ccgttaactt cccctttcac attgatgaag tcaggtgaaa
    12241 tttctttcgc actggtttgc aggcaaatcg ctccctctcc gcgcaggcgt ttcactttca
    12301 gttgcacatg cgttgccgca ccagtgatca gccacagttt tccgccattc agattgatgt
    12361 taccaacaat gaaagtgacg ctgtcacctt ttaccgtact gccgtcgaga atcaacacac
    12421 cgccctgctg tacagtaatc gttgattgtt gcagctctgc gccataacca ataatcattg
    12481 aagcgccgtc cagtaatgca acactcccgg tcagttcaac cggtgcgcga tcttcctcta
    12541 cgacaaaatt ttcgtccgcg ctggtgggtg acaacaggat cactgttcca ccttgtatta
    12601 ccgcattttc cagcgtaccg gcatgcactt cagcccgccc ccccacattc accgacaggt
    12661 tttgcgtctt accggaactg tagagctgaa ggccatccgt ccccagacga taaatggatc
    12721 cattctcaac aatagtgtca gagtcttcac caccgagatt attcatggtg ccaccagcaa
    12781 gcaacgtatc actggctttc ccggccagca taaaaacgcc gccctgatta atctcacaat
    12841 tgctggcttc tccacttgga tacaccaaca attcaccacc ttcatctacc acgaccgctg
    12901 aggttgtccc attaaccaac aggccgccct cttgatcgag aatgattttt tccgcgcgat
    12961 gtccttccag tacacgcagg ttaccgccat tttccagcaa caaaccgcag gcataaccct
    13021 gatcaacgct gaattcacct tcgggatggc ggccattaac cgtagcgagc gtagagagac
    13081 taattgcgcc tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg ctgaccgata
    13141 gcgtgccatt gttaccgtac tgtgtgcatt tggcgatccc accggttgcc actttcaacc
    13201 agccagcccc cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca atcaactcgc
    13261 cttcagtaag ttgcgtcgcg ccaaggacct cgccggtctt aacaatttgc acattcccgt
    13321 cgctcatcaa gtttttcgca tcagatccca tgatttattc ctttgctgca tctgtgtgcc
    13381 tttattgcta cctaagtgta aaggctacgg aggatttatc cacgacagat ttgagatggt
    13441 ggcaaacaac tctgtttaaa ctctgataca cgaattattg ggttgtatca gatgtaaatg
    13501 cgatcctgaa taaaaatcgc ccttgcaaat caacaaaata tagccaattt gcttaaaagc
    13561 tctgcaaacg gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac ctttccactc
    13621 tgccgctaat attcgtcccc gttgtcacct acaacgttgc gttcatagct cagttggtta
    13681 gagcaccacc ttgacatggt gggggtcgtt ggttcgagtc caattgaacg caccatcctg
    13741 cgtccgtagc tcagttggtt agagcaccac cttgacatgg tgggggtcgg tggttcgagt
    13801 ccactcggac gcaccagatt ttcttaatct ggtcttttcc tttttccctc tgtttcttct
    13861 ctgtatccaa tacgttaaaa gatttacact gtcttcgtat gcgttatcag aaggagaatc
    13921 gctatggcaa ctttgttaca acttcatttt gcttttaatg gcccatttgg cgacgcaatg
    13981 gctgagcagc ttaaaccact tgctgagtcg attaatcagg aacctggttt tctgtggaag
    14041 gtatggacag aaagtgaaaa gaaccacgaa gccggtggga tctacctttt cactgatgaa
    14101 aaaagcgccc ttgcctatct ggaaaaacat actgcccgac tgaaaaatct cggcgttgag
    14161 gaagttgtcg ccaaagtttt cgatgtcaat gagccactta gtcaaatcaa tcaggcaaaa
    14221 ctcgcctgac agaatttaat caagggcggt tagcgccctt ttcatccctg tctgaaattt
    14281 ctcaaattct aaaaatctca accaaactta tctgataaca ctaaattcga aagaatgcgt
    14341 acaggtaagt aacaatgaaa aaaattgcta ttgtgggtgc cgggcctacg gggatctaca
    14401 ccttattctc gcttctacag caacaaactc cactttctat ttctatcttc gagcaggctg
    14461 acgaggccgg tgtcgggatg ccatacagtg atgaggaaaa ctcaaaaatg atgctggcaa
    14521 atattgccag tattgaaata ccgccgatta attgtacgta tctcgaatgg ctacaaaagc
    14581 aagaagccag ccatctccag cgttatggcg ttaaaaaaga aaccttgcac gatcgtcagt
    14641 ttttaccgcg aattctgctg ggcgaatatt tccgcgatca atttttacga ttagtagacc
    14701 aggcacgaca gcaaaaattt gcagtggctg tttatgaatc atgccaggtt accgatctgc
    14761 aaattacaaa tgctggcgtc atgctcgcta caaatcagga tttacccagc gagacgtttg
    14821 atttagcggt gattgccacg ggtcacgtct ggcctgatga agaagaagca accagaacgt
    14881 attttcccag cccgtggtca ggcctgatgg aagcaaaggt cgatgcgtgt aacgtgggta
    14941 ttatgggaac atccttgagc ggactggatg cggcaatggc agtggctatt cagcatggtt
    15001 cgttcattga agatgataaa caacacgtcg tttttcaccg cgataacgca agtgaaaagc
    15061 taaatattac gttgatgtcg cgcacgggta ttttacccga agccgatttc tattgcccta
    15121 ttccctacga gcccttacac atcgtcaccg atcaggcatt aaatgctgag attcaaaaag
    15181 gtgaagaggg ccttttggat cgggtattta gattgatagt agaggaaatc aagtttgctg
    15241 atccagactg gagccaacgc atagccttag agagcctgaa tgtcgattcc tttgctcaag
    15301 cctggtttgc cgagcgcaaa caacgcgacc catttgactg ggcagaaaaa aatctccagg
    15361 aagtcgaacg caataaacga gaaaaacata ctgttccctg gcgttatgtc attctgcgcc
    15421 tgcatgaagc cgtacaggaa attgttcctc atctgaatga acacgaccat aaacggttca
    15481 gtaaaggcct tgccagggtt ttcatcgata attatgcggc aatcccttca gagtctattc
    15541 gtcgactact ggccttacgt gaagcgggga tctgaaatcc ctgatgttgg cgaagattat
    15601 acgttacagc aacccgaaga tattcgtgga cgcgtagcgt tcggcgcgtt gccctggttg
    15661 atgcacgacc agcctttcgt tcagggactt acggcatgtg cagaaattgg tgaggcgatg
    15721 gctcgggcgg tcgtaaagcc tgcatcccgt gctcgtcggc gtctttcgtt tgattaaagg
    15781 taaagctatc agcactcaac aaactgatgc tgttcattaa gacgatagcg gactccggtt
    15841 cgaatattgt tttcaccttc aatggcgacg gcgaagcgaa ctctttcgcc gtcatgatat
    15901 gccagtgcgg cgcttccccc tagcccgaga ataattgagt cgaccacgcc cgtgctcact
    15961 accgttgaat tttcaccgct ggcaatgatg tgtacattgt cgccgctgtt ggcaattctg
    16021 gcgtttgcgc caaagctggc gatttgcacc aggtcaccat tactggcgac atgacaccgt
    16081 tcacccaatg tacaaacgcg tactcgcatt ccggtattcg caatccgact actatctcca
    16141 gcgctgctta tccgcgaaga attaccggtt acagcaaccc gtgcgcgttc accggagctg
    16201 ccaatgtgac tgttaaagcc gacacttcca atccgcacgc tataacccat actggcaatt
    16261 tgcgcggcat acccagcggt tgcgattcgg gcgttatcct cacaacagcc gatgcgagca
    16321 tgatcgtcgc tgcgggcaaa ctgactgcaa aatggtatct ctccggggtc tgtggccagt
    16381 ttttgcatag aggaaacttc ctgatgcgcg aagttttcat ccgccagcca ttttttccac
    16441 gcatactcaa ccagactctc cgcccagtcc gtatatcctt cctcaatcag tgcatgacga
    16501 atatcggcat aactaccgcc gcgtgggaaa tggcgcagga accagcgata catcaccgct
    16561 ccgatacgcc attctcgcag gtctgctcgt gtaatgatca ttcgttagtg ccggtgatag
    16621 ccatcgacca tgcttttaaa ggtttcgtgt ggcgtacgcc ccgtggtgca aagataaaga
    16681 tgaccgaaga taaaaaagag gcttataaat gccagagcaa aatgcgcctg caataaccag
    16741 tatcttacgc caggaaacac atctcccacg gcttgcggat agagacacaa cagccccgtc
    16801 aatagtaaca acggcagcaa tccatacatg acaccaacat aggcgacctg ctgtaaggga
    16861 ttaaatttag actgtgttgt tgccgggaaa ggatgttctt ccccctgcat aatgccaaac
    16921 aaataaaatc gcgtttgttt tgccgctcgt tccagccacc cctgacgacg aatgcgatag
    16981 tggtgaccat tatccccaac ggcattgatc agcacaaagc cgagccagca tgccagtaac
    17041 aaaaatccgc aaacttcatg caccgcaacc agacttttaa ccgcagttgc gcccaccatc
    17101 gcaaagtgat ttatcagccc acttgccagc aacaatacaa agagtaacgc attcgaccag
    17161 tgccataatc tgaccgcttt gctgtaaaga taaaccttct caccatgatc ggtggctgat
    17221 ttcttcacgc cacgagcacg aagcaaggcg tgcagcccca gcaccaacca catcccaacc
    17281 agcaatactc cggcaatgat caaccacacc ggccaaaatt cgggagtgaa ctgtggcaca
    17341 tagttcgcta actggctctg aaactgttca gcatgttgcg acgggttcat acattttcct
    17401 ttttaatcaa atgttgaccg aaccggcgat acagatgcgg ttttccggca ccaggtagct
    17461 gatattgata gtatttatta tcctgcaacc acgcctgaat ttctgggcta tcttcgcgac
    17521 caaagatcaa tgcgtgttcc ggacaggcgc tgacgcaaat gggcggaaag ccttttgcca
    17581 gtcgggactc agcgcagaaa tcacatttat ctgccacttt agtgacaggg ttaaggtagc
    17641 gaaccaggta cggacaagcg ccaatgcaat aactgcagcc gatgcactgc gatttttcta
    17701 cccgcacgat cccctgctca tcacgccacg atgcccccgt cgggcatacg tcgatgcacg
    17761 gagcatcttc gcagtgctga catgactggc gaaaaaagtg atactgcgtt tcgttgtcgt
    17821 tatcggtgac tggaatgtgt gctatcgata aacggcttcc ctgagcgggg acgtggttag
    17881 ttttacggca tgcgcgggcg caaatattac agccattgca ccgtgactcg tcatgaatca
    17941 tggcataccg aacttccttt tcttgcctcg tgttcgctaa cagagaagac gcagaaccag
    18001 taaaaaagat tactgttccc atccccagaa caaatttgcg tcgagtgaag gacatctgat
    18061 tatcccctaa tgcagtggct gcaactgtcc accctatcca tgacatctcc ctgaaatttc
    18121 aggggttctg aaactgctct acctcgaacc attcgcataa gccataagcc ttgccatagg
    18181 tcataaatcg ctcgctaaac aagtacaaca ccgccgtcga tgactccacc tgccgtatat
    18241 cggcatattc aggtgcaaca tccatcgcgg cgatcgcggc ttcaatctgc gcttcctgga
    18301 agtaatatgg cgcttgcatc agcatggcga ctttgtaagg acgaggatac gtctggcact
    18361 caaaacgaac ggcgtgtgca atggcgcgac aaatgtcctg ctccacaact tgcaaagaca
    18421 tcgccgcgta gttttcactc atggcctgcg ttgagtaata gtaatcatcc tgacttcccg
    18481 tcatacaagc gatatccgcg tagtctccgt tctgcttaat gctttccagc aaaactgaca
    18541 gttcctcttc tgcaaccgaa tagggtggcg agagaaacac ggtgcgcgcc accagctgcc
    18601 cagccgcaga atgctggcga atgaactcag ccaggatttc agcaggttct ggttcggtta
    18661 ccgtatcaac ggggtgacgc atcccctgca accattgccg cttcgcctcg tcaacttcgc
    18721 tgactttcgc taacggcaat tcatcctgat ggttcatctt acccattcct tacgcaggca
    18781 gtagattgtg tgctgccaga tcggcggcaa tatcttccag ccccagacgc tgcaatgttt
    18841 cgcgggttgg acaaccaagc tgtgggtccc agcccatttc tttgtagaac atcgtgagcg
    18901 aagcatgcat atcgtcacga tccattttgt cagtaccttc agtaaagacc gggatctgcg
    18961 gatccttgtc gaaaacccag gaacagataa gatcgtgttc attacgcata tctttggttt
    19021 gcatcggttt taccgtgtag gcacgatgca acgtaaagat acgctctgca gctaaatcta
    19081 atttttcctg agtcatctct tcgccggtga tcgctttgaa gaatttggct tcaagcgcca
    19141 gatcgccccg gtaattacgg cttttcagcg gcgaaacggt cattggccag acccagttgc
    19201 acagagtgac ggcgttatgc aaacagaccc gcaacagcga ccactttgca tatttaattt
    19261 ttgcgtcatt gattggcgtg tagtttttgg tttcatcgta agcatcttca gaaccaaaaa
    19321 gttctttcgc cacttcacgt tgcagtttca atggcaagcc ggaaccaata aagttgatat
    19381 gggtatgcgt catgcagtca cggttgaaca tacacttaac aatggaaccg acctgcgctg
    19441 acgcttcatt ggcatggtga accggatagc caaacggcga ccagagttta tttttcgcgt
    19501 agccccagta ctctttaccc aaattccagc gttctgcgat ggcatatgaa ccatcagcca
    19561 ggtgactcag ctcacccaca cgatgcgcca gacggtagta aaaatcttta atgaagttaa
    19621 cgtcacccgc ttccagttga tcccagcgaa tttctgcata ctcttcagct ggcagaacac
    19681 gcttgaacac acctttgctg taacaataag taaagtcgcg atgcaactgc ccgtagttac
    19741 accataggcc gtagtcatcg aacaaattca gaccaaccag gttaccaatc acacgaccat
    19801 cgtctttatc ttcaaaatct ttcggcccgt tcgggaagat ggtggtatgg acaaagtttg
    19861 ctacacaagt gttaccacct gtgctgggca cgccaaactc tttcacccga ggaatattca
    19921 tttgggtcat acaacggatc gggcaagagt ggcagccgct catttttact gtgtatttct
    19981 ctgccgccgg tcctaagtca aaaacggatt tataggtacg aaagccgacc gtattctgat
    20041 tgcctggcgg aatttcaccc gtttcaatcg gaccaccttc agccgcgccc caaaacagcc
    20101 ctttacgtgc tgtccagcgt gacttgggat ctgcgtactt ggcacgacat ggttgttatt
    20161 cgcaccaata agttcagtca tcatgtaatc attgagacgc ttcatctcct gacgatcggc
    20221 aatgttgacc cctttcgtcc cttcaaccgc aatcgctttc aggtttttcg aacccattat
    20281 tgcgccagtt cccgcaccgc cgctgtggtt acggctattc aacatgccag agagaggaat
    20341 aaggttttcc ccagcctgac caatagccgc cacacaggtt tccgggctgg tcaatcgaca
    20401 aatttcttcc gtcgttgcgc gcgtcccttt tccccataag aaatcggctt tttccaggct
    20461 aactttgtca tctttaatct tcagccatac cggtgatttc gccttccctt cgataataat
    20521 gacgtcgtat ccagcgaatt tcatttgcgc tgcaaaaaag ccacccatat gggcatcgcg
    20581 actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga actacagggg
    20641 gcaccagatc caggtaatgg gccggtagca aagactaatt tattcgcttc atcgaaaggt
    20701 ttcgtgccta gcggtacttc gtcatacata attttgtagc cgaagcccat gccaccgaca
    20761 aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag attgactctt
    20821 aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt cacacagtaa
    20881 tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg cattcaccgc
    20941 ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc atccacgggc
    21001 atgccgtagt acaggcgcta cagccaatac aacgtttatg atcgacggta atacagcctt
    21061 ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc ggctctttgc
    21121 attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg ccagagccaa
    21181 ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag aatgtcccta
    21241 ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc gtgcatcgcg
    21301 ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg ccgctatcaa
    21361 tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt agaccagcaa
    21421 ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct aatagtggac
    21481 gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga caattcagca
    21541 gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac cagatatttt
    21601 ccctaacccg atagtgtatg agaaaagatt taacgccgat aaactaatcc tttatggtta
    21661 ggagaaaaaa taatgtgatt attctgagcc cttaacattg atcgttatca attaaaatta
    21721 caaagcaaga aattataagc gaactgatat ttattatcat ttgaaataaa tttaacttaa
    21781 gcgacaatca tttgtgacgt tcattgcaga aaataaaaat aacattatcg ctataaatta
    21841 aaatacatac cgaaattcgt ataatttcag cagttgttat ttagaatgat taactggacc
    21901 gcaactgaag caagaatatc aggatgaagc ttaccgcctc atcctgaatg gtcattttac
    21961 cagtgcgtta atgacggttt ccatcgcctg cttttccagc tcactgcgct gaatgcgttt
    22021 attggctaat gctgtacgta atacgcctgc gataacaata tgctttggtt cctgccccag
    22081 atcgcgcatt tccagcacaa ctttaccgac aactctgcac atttctcggt acagttcatc
    22141 ctcttttgtc cgatttccca ttctttatac ctatttatca tgccaacta
//
LOCUS       DAJTRN010000066        21302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRN010000066 DAJTRN010000000
VERSION     DAJTRN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="OO328_002593"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="OO328_002593"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5931113.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="OO328_002594"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="OO328_002594"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5931114.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="OO328_002595"
     CDS             complement(3122..3421)
                     /locus_tag="OO328_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5931115.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="OO328_002596"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="OO328_002596"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5931116.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="OO328_002597"
     CDS             complement(4633..5070)
                     /locus_tag="OO328_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5931117.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="OO328_002598"
     CDS             complement(5116..5457)
                     /locus_tag="OO328_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931118.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="OO328_002599"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="OO328_002599"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5931119.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELVRAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="OO328_002600"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="OO328_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="OO328_002601"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="OO328_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5931120.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="OO328_002602"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="OO328_002602"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5931121.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="OO328_002603"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="OO328_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5931122.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13218
                     /locus_tag="OO328_002604"
     CDS             12688..13218
                     /locus_tag="OO328_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5931123.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13330..13866
                     /locus_tag="OO328_002605"
     CDS             13330..13866
                     /locus_tag="OO328_002605"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5931124.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13974..14798)
                     /locus_tag="OO328_002606"
     CDS             complement(13974..14798)
                     /locus_tag="OO328_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5931125.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15118..17148
                     /gene="malS"
                     /locus_tag="OO328_002607"
     CDS             15118..17148
                     /gene="malS"
                     /locus_tag="OO328_002607"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5931126.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17326..18579
                     /gene="avtA"
                     /locus_tag="OO328_002608"
     CDS             17326..18579
                     /gene="avtA"
                     /locus_tag="OO328_002608"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5931127.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18730..19203)
                     /locus_tag="OO328_002609"
     CDS             complement(18730..19203)
                     /locus_tag="OO328_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5931128.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="OO328_002610"
     CDS             complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="OO328_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5931129.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20353..>21302
                     /gene="yiaK"
                     /locus_tag="OO328_002611"
     CDS             20353..>21302
                     /gene="yiaK"
                     /locus_tag="OO328_002611"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5931130.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRR"
BASE COUNT         5357 a         5437 c         5222 g         5286 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaaccc ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcaccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaaatgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgccttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgagc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcacccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc
    13081 ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag
    13141 tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa
    13201 aatcggctgg cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact
    13261 gacgcccgag cgcggcatat tctgcaagta tgtgaacatc tacatattcc cgtaccggaa
    13321 aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc
    13381 ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa actgttgcat
    13441 cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc
    13501 attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat tcaggccatg
    13561 cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc
    13621 gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat
    13681 gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg
    13741 tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt
    13801 tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg
    13861 ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg
    13921 cgagcaaaag ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata
    13981 tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc
    14041 ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga
    14101 tccgctttac gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc
    14161 aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc
    14221 actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta
    14281 ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc
    14341 atactggtag ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta
    14401 ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca
    14461 ggtgcccatt ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct
    14521 gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt
    14581 ccggaagggt attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac
    14641 tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct
    14701 gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga
    14761 atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg tcggatttaa
    14821 tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa
    14881 atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg
    14941 attcagattg gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt
    15001 tgcgcgcgat cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt
    15061 tgtctggctt atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg
    15121 aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact
    15181 tctccggggt tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag
    15241 ttgcccaaag gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca
    15301 gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa
    15361 tggcgattgt tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca
    15421 acattgatga tttccatcca gaacgccgcc gaaccggtag caaacctggt ccgtgaatgc
    15481 ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc
    15541 gccgtacggg attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta
    15601 caacccgctg ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc
    15661 gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac
    15721 ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc
    15781 acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg
    15841 ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggt
    15901 ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg
    15961 aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat
    16021 cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg
    16081 gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa
    16141 acgctgggtg aacgctggag cgactggaaa cctgccgccg gacaaacctg gcatagcttt
    16201 aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg
    16261 atcagaacgg atatcggcga ttacgacaat cctggattcg atgatctcac tatgtcgctg
    16321 gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat
    16381 aaaaacaaaa cggatactca cgctaaagtc atcgaaggct ttacacctcg cgattactta
    16441 acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc
    16501 gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt
    16561 cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc
    16621 ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg
    16681 atgatcaatt tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg
    16741 gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc
    16801 tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta
    16861 ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct
    16921 acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt
    16981 aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc
    17041 gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt
    17101 gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt
    17161 cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg
    17221 ttgcgggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc
    17281 gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt
    17341 tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac
    17401 ggtttacgca cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa
    17461 atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat
    17521 gcactgtgta actacgacgg tccacagggg aaaacggagc tactcacact gcttgccgga
    17581 atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc
    17641 agccagagcg cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg
    17701 gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg
    17761 gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt
    17821 aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc
    17881 tcccggccga cgaatccaac aggtaatgtg attactgacg aagagttgct gaagcttgac
    17941 gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc
    18001 ccgggtatca tcttcagtga agggcgcccg ctatggaatc cgaatatcgt actgtgcatg
    18061 agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa
    18121 atcatcactg ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt
    18181 ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc
    18241 atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta
    18301 ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt
    18361 aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg
    18421 atggtgccgg ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa
    18481 tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg
    18541 gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga tgcgctacgc
    18601 ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata
    18661 aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcattg agaaacgccg
    18721 gaaaacgttt caaacaggct gctgctgtag ccgatgccgc tgcactttct cgacatccat
    18781 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca
    18841 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
    18901 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat
    18961 atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc
    19021 acactgatga caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga
    19081 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
    19141 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
    19201 catgatcatt cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
    19261 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgccc
    19321 aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt
    19381 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
    19441 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
    19501 agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg
    19561 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
    19621 ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
    19681 gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt
    19741 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg
    19801 gtttcaccag tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc
    19861 agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct
    19921 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
    19981 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
    20041 ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc
    20101 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
    20161 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc
    20221 acttgatttt tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga
    20281 tatatttgaa atctagttcc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    20881 atgttctctt acggcatgtt agaagttaac cgcctggcag gccgtcagct cccggtcgat
    20941 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    21001 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    21061 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    21121 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    21181 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    21241 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    21301 aa
//
LOCUS       DAJTRN010000067        20811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRN010000067 DAJTRN010000000
VERSION     DAJTRN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..700
                     /locus_tag="OO328_002612"
     CDS             <1..700
                     /locus_tag="OO328_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5931131.1"
                     /translation="HENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVK
                     RYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVI
                     SYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSM
                     SRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGL
                     TPIEYRNQTYMPRV"
     gene            802..954
                     /gene="hokA"
                     /locus_tag="OO328_002613"
     CDS             802..954
                     /gene="hokA"
                     /locus_tag="OO328_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5931132.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1141..1353)
                     /gene="cspA"
                     /locus_tag="OO328_002614"
     CDS             complement(1141..1353)
                     /gene="cspA"
                     /locus_tag="OO328_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5931133.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1634..1924)
                     /locus_tag="OO328_002615"
                     /pseudo
     CDS             complement(1634..1924)
                     /locus_tag="OO328_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2358..3068
                     /locus_tag="OO328_002616"
     CDS             2358..3068
                     /locus_tag="OO328_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5931134.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3118..4092)
                     /gene="ghrB"
                     /locus_tag="OO328_002617"
     CDS             complement(3118..4092)
                     /gene="ghrB"
                     /locus_tag="OO328_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5931135.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4196..4846)
                     /locus_tag="OO328_002618"
     CDS             complement(4196..4846)
                     /locus_tag="OO328_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5931136.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5009..7207
                     /locus_tag="OO328_002619"
     CDS             5009..7207
                     /locus_tag="OO328_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5931137.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7176..7616)
                     /locus_tag="OO328_002620"
     CDS             complement(7176..7616)
                     /locus_tag="OO328_002620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5931138.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7613..8176)
                     /gene="tag"
                     /locus_tag="OO328_002621"
     CDS             complement(7613..8176)
                     /gene="tag"
                     /locus_tag="OO328_002621"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5931139.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8334..9032
                     /locus_tag="OO328_002622"
     CDS             8334..9032
                     /locus_tag="OO328_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5931140.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9261..10469
                     /locus_tag="OO328_002623"
     CDS             9261..10469
                     /locus_tag="OO328_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5931141.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10793..12484
                     /gene="eptB"
                     /locus_tag="OO328_002624"
     CDS             10793..12484
                     /gene="eptB"
                     /locus_tag="OO328_002624"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5931142.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12576..12652
                     /locus_tag="OO328_002625"
     tRNA            12576..12652
                     /locus_tag="OO328_002625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12610..12612,aa:Pro,seq:cgg)
     gene            13396..15003
                     /gene="dppA"
                     /locus_tag="OO328_002626"
     CDS             13396..15003
                     /gene="dppA"
                     /locus_tag="OO328_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5931143.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15311..16330
                     /gene="dppB"
                     /locus_tag="OO328_002627"
     CDS             15311..16330
                     /gene="dppB"
                     /locus_tag="OO328_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5931144.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16340..17242
                     /gene="dppC"
                     /locus_tag="OO328_002628"
     CDS             16340..17242
                     /gene="dppC"
                     /locus_tag="OO328_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5931145.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17253..18236
                     /gene="dppD"
                     /locus_tag="OO328_002629"
     CDS             17253..18236
                     /gene="dppD"
                     /locus_tag="OO328_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5931146.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18233..19237
                     /gene="dppF"
                     /locus_tag="OO328_002630"
     CDS             18233..19237
                     /gene="dppF"
                     /locus_tag="OO328_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5931147.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19267..20538)
                     /locus_tag="OO328_002631"
     CDS             complement(19267..20538)
                     /locus_tag="OO328_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5931148.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5041 a         5114 c         5443 g         5213 t
ORIGIN      
        1 tcacgagaat agaggccgat acggataccg tagggtaacg ctgtctcttc atcgagaagg
       61 gaaacagatt aaccataaag ctgttcagcg cctgatggga accctctcac ttaaagcagc
      121 gattaaggtc aagcgatacc gctcttacag aggagaggta gggcaaaccg cccctaatgt
      181 tctccaaaga gatttcaagg ctacgcggcc aaacgagaag tgggttaccg atgttactga
      241 atttgcagtc aatgggcgca agctgtattt gtctccagta atagatctct tcaacaacga
      301 agttatttct tacagccttt cggaaagacc agtgatgaac atggttgaga atatgctcga
      361 tcaggcattc aaaaagctta atcctcacga gcatcctgtt ctgcactctg accagggatg
      421 gcagtatcgt atgagaagat atcaaaatat ccttaaagaa catggtatta aacaaagcat
      481 gtccagaaaa ggcaattgtc tggataatgc tgtggtggag tgtttctttg gaaccttaaa
      541 gtcggagtgt ttttatcttg atgagttcag taatataagc gaactgaagg atgctgttac
      601 ggaatatatt gaatactata acagcagaag aattagcctg aaattaaaag gtctgactcc
      661 aattgaatat cggaatcaga cctatatgcc tcgtgtataa ctgtccaact ttttggggtc
      721 agtacaaagt gaggctaccc tatgcctgaa caccataagg ttagcctctt actcgttgga
      781 aatcaacaca gggggctggg aatgccgcag aaatatagat tactttcttt aatagtgatt
      841 tgtttcacgc ttttattttt cacctggatg ataagagatt cactgtgtga attgcatatt
      901 aaacaggaga gttatgagct ggcggcgttt ttagcctaca aattgaaaga gtaagagtct
      961 tcgacgggaa attattcccg ccttacttac ggcgttgcgc attctcattg cacccatatt
     1021 tattcttcac aaaaataata atagatttta ttacgcgatc gattatttat ttcctgaaaa
     1081 aaaataaaaa aatccccgcc aaatggcagg gatcttagat tctgtgcttt taagcagaga
     1141 ttacaggctg gttacgttac cagctgccgg gcctttagcg ccgctttcga tggtgaagga
     1201 cactttctga ccttcgtcca gagatttgta accatcgttc tggatagcag agaagtgtac
     1261 gaacacatct ttagagccat cgtcaggagt gatgaagccg aagcctttgt cagcgttgaa
     1321 ccattttacg ataccagtca ttttaccgga catagtgtat tacctttaat aattaagtgt
     1381 gcctttcggc gatatggcgt gctttacaga ttttgaagcg ttaaaggaat gtgcactacg
     1441 aggggtatca acgataactc ttgaagggac ttgccttact acactgcttt aatggtctgt
     1501 acgtcaaacc gttgatgtgc attaagccac tcattggcgg gtgatgcaac aattattttt
     1561 catatttatg attaatcggt tggaaaacgg tcctgtcatc aggaccgtaa acagcaataa
     1621 agtggataaa agtctatttc atcaactgct tacttaatgc cgggttggct tgaatcaaac
     1681 gcatcaattt tagttcggca cttgaaggct tcacgcgtct ggattcccat tcctttacca
     1741 tggcgactga tacgcccaaa acccgggcga aatcatcgat ttttaatcct gtcccttttc
     1801 ataactgctc aatttcggta caggacgttg ttctgtgcgt cagggtaatt ttctgcgttt
     1861 catctttaaa aacaatctgt tccaggctgc tcaacagctc atgcattgga tctttatatt
     1921 ccattgaaaa ctcctcaaat cacactgcgg gatcgtgaat tgcatcgaag ctcattaaga
     1981 atagtcggga aaagcagaca cagcggggtg aagtcagggc atgctctggc tatgattcga
     2041 ttcagcctgt catttagcgt gacttatagc cacctaacga tctgataaat gccgatatat
     2101 ctcttatcac tcctgttttg taaagatttc gcaaatctaa ccttctgcga aggcgtaaat
     2161 ctctggctac aaaaaaggca acggcactat cattttttaa caatgaaaca acaaattaac
     2221 cttgtttcat tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag
     2281 cctgtcctgg actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg
     2341 gtgaaaggag taagaaaatg gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg
     2401 cggcgttatt aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta
     2461 aagcgtttat cgacttcctg caaaatacag tgatgcgtag cggtgaacgt ctaccaaccc
     2521 tgactgccga tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg
     2581 gttattctca gcaggtgaat caggcgatgg attccggtct gcgcccggtc gtagacagcg
     2641 ttaacgcaat tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga
     2701 atggttcgct gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg
     2761 cgcactctgc gctaaaacag agcgatgacc tgaaaccggt atttgatcag gcattcacca
     2821 aagtggtaac gacgccagct gatgcattgc aaccgttgat tccggcggca caaactttca
     2881 cgcaacaact ggtgatggtt ggagactaca ttgctcagca aggtactcag gtgagctttg
     2941 tcgcaaatgg catccagttc ccgacttcac agcaggcaag tgaatataac aaactgattg
     3001 cgccattacc ggcacaacat caggcgttta atcaggcctg gactacagca gttactgcaa
     3061 cccaataaag agtaaaagcc cgagcgatta tcgggcttta cttcacgcag tcgcagctta
     3121 gtccgcgacg tgaggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa
     3181 attatccacg gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg
     3241 cggtactgcg acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg
     3301 ttcgaagaca tccagcccgg cggcgtgaat ttcgcctttc tgcaatgctg cgatcagtgc
     3361 attttcgtca accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt
     3421 ggcgaattgt tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca
     3481 aacgaaatct gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc
     3541 ttctgcttct ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg
     3601 cacacgttgt gccagcgcca tgccgatccg tcccatcccg acaatgccca gtgttttatg
     3661 gtgaacgtca gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc ctgcttttac
     3721 ccgttctgct acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc
     3781 ggcgacggtt tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag
     3841 cgcatcgaca tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg
     3901 cattttttcc agcaatgcgg catctacatt ctcgtttgaa cccagtaaac cttcagcttc
     3961 tgcaaaaatt gctgcatttt gaacgacggt ttgtgggctg aggtttgcca cctggtgaac
     4021 ggtgaaatgc gcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac
     4081 ggacggcttc atgcttctct ccattcactg ataaggcgat caccttagcc tgaaatatga
     4141 tgaaggaaaa ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca
     4201 gcgggcttaa ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc
     4261 tggcgattgg gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct
     4321 gggtgatcaa cgcgctggca acggaatccg cacgctgctg ggagagacgc atgttcaggt
     4381 cgtgaccccc cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt
     4441 tcagcaccat tgccacgccg gtcagggtgt tagcgcctgc cggtttcagg ggagcgctgc
     4501 tgctgtcgaa ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta
     4561 cgctaacacc agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca tccatgtaat
     4621 aacccacgcc gccgcccaga gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc
     4681 gatctttctt cgaagaagag agcgcaccaa tacccgcgcc cacgagagag cccagacctg
     4741 cgccgatagc agatttgcct gcttcgcgtt cgccggtgta agggttagtt gtgcagccag
     4801 ataccgccag agcgccactc actacggcgg caagtaagat aaacacgttt cttcattgtt
     4861 aatccttaat aaccttttta ttctttgtca cgggttccgt ggcgggagat tatgccgcgt
     4921 gaacatgaag attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa
     4981 tacatctaac ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct
     5041 gactgccgcc cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc
     5101 gcgtggcgcg ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt
     5161 tcacagcaat acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga
     5221 aaacccgcaa ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct
     5281 ggcaggcggt tataccgggc atctggggga ttattcgacc ggtgcggcac aggcgatcat
     5341 gccgtatgtc gtaggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct
     5401 ggaacatagc gatgtcgtgg tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc
     5461 gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg acagtgggaa
     5521 aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
     5581 ggagtgggtg gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac
     5641 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc
     5701 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaacg ccgaatgggc
     5761 ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca
     5821 aaataccacc atgctgatgg caggctgggg aatgcagcgc caacagtttg gtgagcaaaa
     5881 acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg
     5941 ttttggtctt tcttaccatt ttgccaatgg tggtgacccc acgcggcgtt ctgcggtgct
     6001 ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc
     6061 ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg
     6121 acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca
     6181 ggataccaat cgtctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg
     6241 cttctggacg gcggcggcga aacacgcgga tattgttctg cctgcgacta cctcttttga
     6301 gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca
     6361 agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttgagtga
     6421 acgttgggag aagggcggct atgcgcggtt tacggaagga aaaagtcagc tgcaatggct
     6481 ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc
     6541 atttgctgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga
     6601 gcggtttatt cgcttcgctg atttttgccg cgatccgctg gcgcatccgt taaaaaccgc
     6661 cagcggcaag attgaaatct tctcacagcg tattgccgat tacggttacc cggattgccc
     6721 tgggcatcca atgtggctgg agccggacga atggcagggc aatgccgaac cagaacagtt
     6781 gcaggtactt tctgcccatc cggcgcaccg cctgcacagc cagctgaatt acagttctct
     6841 gcgcgaattg tacgcggtag caaaccgtga gcctgtcacc attcatcctg acgatgctca
     6901 ggcgcgcggc ataaccgaag gggatatggt gcgggtgtgg aactcccgtg ggcagatcct
     6961 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc
     7021 atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct
     7081 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cgacgctggc
     7141 atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
     7201 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa
     7261 ccctgcgcac ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga
     7321 tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc
     7381 ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga
     7441 agcttaccgt cctcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc
     7501 agcggaatgc agtcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt
     7561 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt
     7621 tatttcccag atagcaacag cagccaacca catgatcatt caccagccca catgcctgca
     7681 taaaggagta acagattgtg gtgccgacaa acttaaaacc acgttttttc agtgccttag
     7741 atagggcgtc ggaggcggac gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca
     7801 cctgtggctg atgatttaca aacgaccaga caaagtcggg aaacggttcg ccgttctgtt
     7861 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttccct cgatggcgga
     7921 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgaccttca
     7981 ccggatcgaa ctgatgaaag taggcgcgat agttttcgcg ttttttgagg acggtgatcc
     8041 acgataaccc agcctgctgc ccttcaaagc agatcatttc gaacagtttt ttactgtcag
     8101 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggccc tgactcaccc
     8161 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
     8221 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
     8281 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
     8341 taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagcgtggta atcagtgcct
     8401 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac
     8461 attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt
     8521 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
     8581 ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg
     8641 ttacaaccgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
     8701 agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct
     8761 taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata
     8821 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
     8881 caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
     8941 atattgccgc ctatgctgcg ctaactcagg cagaaaatac cactaataat agcgactatc
     9001 tgtatacgat gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa
     9061 taacaacagt tgtggcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat
     9121 tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc
     9181 gtttttcccc aagcataaaa tgcacttcgt tatggttgtc ggcagagatt tttccttttt
     9241 attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac
     9301 tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacccgg agcctgttta
     9361 atggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct
     9421 tgttaagtct ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg
     9481 gcgttaaacg cgtcaccgtg gcttccggca ttttgctggg attaggcttc ttcctgaccg
     9541 cgcattccaa caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg
     9601 atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggttcccg gagcgtaaag
     9661 gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta
     9721 tcgacacgca gctgctcgaa acggtgggtc tggaaaaaac ctttgtgatt tggggagcga
     9781 ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag
     9841 tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac
     9901 cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga
     9961 ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca
    10021 atgcagtaac ggttatttcc atcgctaacc tttcaggtcg tctggtgctg ggcattctgt
    10081 ctgacaaaat tgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta
    10141 tggcggccct gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg attgcctgcg
    10201 tggcatttaa ctttggcggc actattaccg tctttccatc actggtcagt gagttcttcg
    10261 gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca
    10321 tttttggttc gattatcgcc tcactgtttg gcggtttcta tgtgaccttc tacgtgattt
    10381 tcgccctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa
    10441 tgttgcgtga ggcgcatggc tccctttaat gctcagccgc gagctactgg gcttcagctt
    10501 ccgtgtgcat caaacgcatc aacgaaatgt ataaacaatg actcgtaaaa tttgggccag
    10561 ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt
    10621 tgtaaatttg tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac
    10681 ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc
    10741 tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata
    10801 catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt
    10861 tatgaatggc gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg
    10921 gaaaggcgtt tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact
    10981 acgtcttcta tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct
    11041 cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat
    11101 catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt
    11161 tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg
    11221 tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggtggt
    11281 cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa
    11341 gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc
    11401 ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga
    11461 atcttccgat aataattcat tgcttaatcc ggcgaagaag tttacatacc aggctccaca
    11521 aaacgttgat gatacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat
    11581 gggtattttc ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc
    11641 ggcgttccgt ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt
    11701 acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc
    11761 ggttctgaag cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt
    11821 ctacagcaac acgatggcag acaacattgc ttatcgtgag cagattggtg cggagccacg
    11881 taatcgtggc aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctagg
    11941 gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta
    12001 cacccagcgt tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag
    12061 cggctgtacc aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca
    12121 ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc
    12181 tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc
    12241 accgccggag cagttccgcg taccgatgat ggtctggatg tcagataaat atctggaaaa
    12301 tccggccaat gcgcaggcgt ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg
    12361 ccgtcacgta gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg
    12421 tggaattaac gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa
    12481 ctaagcggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg
    12541 agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg
    12601 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa
    12661 aagcctgctc aacgagcagg tttttttgca tttgtagctc atgaggatga gaacctccgg
    12721 gggcagggag gctcgactcg acgtaggcct gataagacgc gccagcgtcg catcaggcaa
    12781 cggcaccgca cttattgtcg gatgcggcgc gaccgcctta ttcgaccaac acaccacaca
    12841 ttttcctgcc aactttgcgc cccatatctc actatccatt aacggatttg tgacagaaac
    12901 cattgtcttt ttttatattt gcttaaatct tttttgcgtt acttatggct ggcgctggca
    12961 ttttccgctg tgcgttttag cgttcatggt attaatcgct cagatatcca ccttttcctc
    13021 agattttttt cattaaatgc ataaaactta atcaccaaat gttgctaaat attaagctga
    13081 ttgttctggt tatgttggag tagcacaaat ttctctgctg caaatagctg tttgaagttt
    13141 ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat cccggttggc tgtattgacg
    13201 ttttcacatt ctgttgacag attgtaggtc acgaggggca ttttatggag gatccgcact
    13261 gttacactga tgttaattag tacggcatcc ccacctcata acgttgaccc gaccgggcaa
    13321 aaaacaaaaa aggtcaggca gcgacaaccc actgcaaagg gttaaaacaa caaacatcac
    13381 aattggagca gaataatgcg tatttccttg aaaaagtcag ggatgctgaa gcttggtctc
    13441 agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta aaactctggt ttattgctca
    13501 gaaggatctc cggaagggtt taacccgcag ctgtttacct ccggcaccac ctatgacgcc
    13561 tcttccgtcc cgctttataa ccgcctggtt gaatttaaaa tcggcaccac cgaagtgatc
    13621 ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta aaacctatac cttccatctg
    13681 cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac cgacgcgcga aatgaacgcc
    13741 gatgacgtgg tgttctcgtt cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt
    13801 tctggcggca gctacgaata cttcgaaggt atgggcttgc cggagctgat cagcgaagtg
    13861 aaaaaggtgg acgataacac cgttcagttt gtgctgactc gaccggaagc gccgttcctc
    13921 gctgacctgg caatggactt cgcctctatt ctgtcaaaag aatatgctga cgcgatgatg
    13981 aaagccggta caccggaaaa actggacctc aacccaatcg gaaccggtcc gttccagtta
    14041 cagcagtacc aaaaagattc ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc
    14101 aaaccgcaga tcgatacgct ggtcttctct attacccctg atgcttccgt gcgttacgcg
    14161 aaattgcaga agaatgaatg ccaggtgatg ccgtacccga acccggcaga catagctcgc
    14221 atgaagcagg ataaatccat caacctgatg gaaatgccgg ggctgaacgt cggctacctc
    14281 tcgtataacg tgcagaaaaa accgctggat gacgtgaaag ttcgccaagc actaacctac
    14341 gcggtgaaca aagacgcgat catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa
    14401 aacctgatcc cgccaaccat gtggggctat aacgacgacg ttcaggatta cacctacgat
    14461 cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac
    14521 ctgtgggcga tgccggtaca acgtccgtat aacccgaacg ctcgccgcat ggcggagatg
    14581 attcaggcag actgggcgaa agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt
    14641 gagtacctca agcgtgcgaa agatggcgaa catcagacgg taatgatggg ctggactggc
    14701 gataacgggg acccggataa cttcttcgcc accctgttca gctgcgccgc ctctgaacaa
    14761 ggttctaact actcaaaatg gtgctacaaa ccgtttgaag atctgattca accggcgcgt
    14821 gctaccgacg accataacaa acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac
    14881 gatcaggctc cggcactgat catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa
    14941 gtcaaaggct atgtggttga tccattaggc aaacatcact tcgaaaacgt ctctatcgaa
    15001 taattaaaag ccatacaaga ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat
    15061 caggcctacg aaaattctgc aatgtattga atttgcacga ttttgtaggc cggataaggc
    15121 gttaacgccg catccggcat aaacaaagcg cactttgtca acaatctgta tacccggtgg
    15181 cgctgtgcct ctgcgtgcag cgccatccgg cagcaatact tcattctctg tccgacaagg
    15241 gcgggaattg atttgtgagc aatacagaca cgcagttcca ggctgcgagt cactatagag
    15301 aatccgggtt atgttgcagt ttattctccg acgtttggga ctcgtcatcc ccacgtttat
    15361 cggtattacc cttctcacat ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat
    15421 catggcaggc gaacgtggga tctcccctga gcgtcacgtg cagctgctgg ctgaactcgg
    15481 cttagataaa ccgatgtggc agcagtatct ccattacatt tggggcgtaa tgcacggcga
    15541 tctaggcatt tcaatgaaaa gccgaattcc agtatgggaa gagttcgtgc cgcgctttca
    15601 ggccacgctg gaacttggcg tctgcgcgat gatttttgct acggcagtcg gtattccggt
    15661 tggtgtgctg gctgcggtta aacgcggttc cattttcgat cacactgcag ttggcctggc
    15721 gctgaccggc tactcgatgc cgatcttctg gtggggcatg atgctgatca tgctggtttc
    15781 ggtgcactgg aacctgacgc ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga
    15841 ctccaatccg ttaaccggtt ttatgctaat cgacaccgcc atctggggtg aagacggcaa
    15901 ctttatcgat gccgtcgccc atatgatctt gcccgccatt gtgctgggta ctattccgct
    15961 agcggtcatt gtgcgtatga cacgctcctc gatgctggaa gtgctgggcg aggattacat
    16021 ccgcaccgcg cgcgccaaag ggctaacccg catgcgggtg attatcgtcc atgcgctgcg
    16081 taacgcgatg ctgccggtag tgaccgttat cggcctccag gtgggaacat tgctggcggg
    16141 ggcgattctg accgaaacca tcttctcgtg gcccggtctg gggcgctggt tgattgacgc
    16201 actgcaacgc cgcgactatc cggtagtgca gggcggcgta ttgctggtgg cgacgatgat
    16261 tatcctcgtc aacttgctgg tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca
    16321 taagaagtaa ggggacatca tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt
    16381 gccgatgacc ccgttacagg agttctggca ctattttaaa cgcaacaaag gcgcggtcgt
    16441 cgggctggtt tacgtcgcca tcgtgctgtt catcgcgatc tttgccaact ggattgcgcc
    16501 ttataacccg gcggatcagt tccgcgatgc gctgctcgcc ccgccagcct ggcaggaagg
    16561 cggcagtatg gcacacttgc tgggcaccga tgacgtcggt cgtgatgtgc tgtcgcggct
    16621 gatgtacggc gcgcgtttat cgctgctggt tggctgtctg gtggtggtgt tgtcgctgat
    16681 tatgggcgtt attctcggcc tgatcgccgg ttacttcggc ggcctggttg ataacattat
    16741 catgcgcgtg gtcgatatca tgctggcgct gccaagtctg ctgttggcac tggtgctggt
    16801 ggcgattttc ggcccgtcga ttggtaacgc cgcgctggcg ctgaccttcg ttgccttgcc
    16861 gcactatgtg cgcttaaccc gcgccgccgt gctggtggag gtgaatcgcg attacgtcac
    16921 cgcctctcgc gtggcaggtg ccggggcgat gcgccagatg tttattaaca tcttcccgaa
    16981 ctgccttgcg ccgctgattg ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat
    17041 ggctgctctt ggtttcctcg gcatgggggc acagccgcca acgcctgagt ggggcaccat
    17101 gctctccgac gtgttgcagt tcgcgcaaag cgcctggtgg gtcgtgacct tccctggtct
    17161 ggcgatcctg ttgacggtgc tggcatttaa cctgatgggt gacggtctgc gtgacgcgct
    17221 cgatcccaaa ctaaagcagt aagaggttcg agatggcgtt attaaatgta gataaattat
    17281 cggtgcattt cggcgacgaa agcgcaccgt tccgcgccgt agaccgcatc agctacagcg
    17341 taaaacaggg cgaagtggtc gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt
    17401 cactggcgat tatggggctg attgattatc cgggccgcgt aatggcagaa aaactggagt
    17461 ttaacggcca ggatttgcag cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg
    17521 aagtggcgat gatcttccag gacccgatga ccagccttaa cccgtgctac accgtgggtt
    17581 tccagattat ggaagcgatt aaagtgcatc agggcggcaa caaaagtacc cgccgtcagc
    17641 gggcgattga cctgctgaat caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt
    17701 acccgcatca gctttccggc ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct
    17761 gtcggccaaa actgctgatt gccgatgaac cgaccaccgc gctggacgtg accattcagg
    17821 cgcaaatcat cgaactactg ctggagctac agcagaaaga gaacatggcg ctggtgttaa
    17881 ttacccatga cctggcgctg gtggcggaag ctgcgcataa aatcattgtg atgtatgcag
    17941 gtcaggtagt agaaacgggc gatgcacacg ccatcttcca tgcgccgcgt cacccgtata
    18001 ctcaggcatt gctgcgtgcg ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt
    18061 tgccaggtgt tgttcccggc aagtatgacc gcccgaacgg ctgcctgctt aacccgcgct
    18121 gcccctatgc cactgacaga tgtcgcgctg aagaaccggc gctgaatatg ctcgctgatg
    18181 ggcgtcagtc caaatgccat tacccacttg atgatgccgg gaggcccaca ctatgagtac
    18241 gcaagaggcc acctcgcaac aaccgctgtt gcaggctatc gacctgaaaa aacattatcc
    18301 ggtgaagaaa ggcatgttcg tgccggaacg tctggtgaag gcgctggacg gcgtttcgtt
    18361 taaccttgaa cgtggcaaaa cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac
    18421 cctcggtcgg ttgctgacga tgatcgaaac gcccaccggc ggcgagctgt attatcaggg
    18481 gcaggatctg ctcaagcacg atccgcaggc gcagaagctg cgtcggcaga aaatccagat
    18541 cgtcttccag aatccctacg gttcgctgaa tccgcgtaag aaagttgggc aaattctcga
    18601 agagccgctg cttattaaca ccagcttaag caaagaacag cgtcgggaaa aagccctgtc
    18661 gatgatggca aaagtcggcc tgaaaaccga gcactatgac cgctatccgc acatgttttc
    18721 cggcggacaa cgtcagcgta tcgctattgc ccgtggtctg atgctcgacc cggatgtggt
    18781 gattgccgat gagccggttt ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct
    18841 gatgatggat ttgcagcagg agttggggct ttcttatgtc tttatctccc acgacctgtc
    18901 ggttgtggag cacattgctg atgaagtgat ggtgatgtac ctaggccgct gcgtggagaa
    18961 aggaacgaag gaccaaatct tcaataaccc gcgccatccg tacacccagg cgctgctctc
    19021 cgcgacgcca cgcctgaacc cggacgatcg tcgtgaacgc atcaaactca ccggtgaact
    19081 gccaagcccg ctgaatcctc cgccgggttg cgccttcaac gcccgctgtc gtcggcgctt
    19141 cggcccctgc acccagttgc agccgcagct aaaagactac ggaggtcaac tggtagcttg
    19201 ttttgctgtt gatcaggatg aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc
    19261 tccggtttac tcaatcagct tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag
    19321 aatcagccag gctttaaatc cgcgaagttt ttccagttgt gcgactttat agatcaggaa
    19381 aaacggaatg aggcacgaca caatgccata taacgggctt cccaactgaa agaacaccag
    19441 cactgataca cgaaacgaaa cccaaatcgt caacgtaatg acgataaaag cgcagatcgc
    19501 cagagtcagc atgcgtgagt taattttctt ggtatcaata attcggctta acagattgag
    19561 aataatgcct ttaatagcct cgtggaaacc gagataaatg ccaaagaatg cggtcagtac
    19621 ggcaaagata ttcagcaccg tagaggtgat atgaatgata tgtccaggga tcacctgcgc
    19681 ggccagtgcc agtgccgaga tattttgttc aaaggcagaa acggcttctt cgtggctaat
    19741 tgagaaggta aacgaaaagg caaaaaacag gatcaccgcg atgagcgtga tataactaat
    19801 tcggtgggta cgcagcgcga gccgggttgc cagtacttta tctgcttccc gtttacgata
    19861 ggcaatattc attgggttaa gtacctgaat aaataccgca gaaaagaagc aaaatggaat
    19921 ggtaagcagg acatcgcgga aaaagacgga ggcttgcggg aaggcggtta tattggcgaa
    19981 attccagtgc gggatcatcg caaaaccgaa cacgacaata atccctactt tgaccaccac
    20041 cattggccca gaaatcttaa acagtaatcg ttcaccacca gacgcaatcg ctatcagtac
    20101 ggcgaaaata gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa
    20161 ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga gagtagataa aaatcccgtg
    20221 gataatcatc aaaaagtaga taaccccgag gaaaattccc cagttcttcc ccaggtaatg
    20281 actgataata tcggtgtagt cattacagga atcgctttca gaaagggttt ttaaataaat
    20341 gtcctgcact acccaggtga caggataagc aatgatcgct gcggtaataa atacccaaat
    20401 tcccttcaag ccaatttgta ctggcatcag cacggttccg gcaccaatcg ccatgcctat
    20461 gcataataaa acccagccga agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga
    20521 tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt cggcctcgca aaaatgaata
    20581 aacccagttt ctcttgcgca acaggtggag aagtcgaata cgcagtatgt aaaaaatgag
    20641 gcaacagatt cactggggag gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac
    20701 taccagtctg gagtcagccc ggatatgaac tgcaactatt gcaaataaag aaattaagct
    20761 ggaaggcatc gcgcaatgag gaagattgcc gggtgctgac agtaaaaagc a
//
LOCUS       DAJTRN010000068        20781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRN010000068 DAJTRN010000000
VERSION     DAJTRN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..319
                     /locus_tag="OO328_002632"
     CDS             <1..319
                     /locus_tag="OO328_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5931149.1"
                     /translation="TDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLL
                     DLFLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGAS
                     GRW"
     gene            complement(379..1677)
                     /gene="eno"
                     /locus_tag="OO328_002633"
     CDS             complement(379..1677)
                     /gene="eno"
                     /locus_tag="OO328_002633"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5931150.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1765..3402)
                     /gene="pyrG"
                     /locus_tag="OO328_002634"
     CDS             complement(1765..3402)
                     /gene="pyrG"
                     /locus_tag="OO328_002634"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5931151.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3630..4421)
                     /gene="mazG"
                     /locus_tag="OO328_002635"
     CDS             complement(3630..4421)
                     /gene="mazG"
                     /locus_tag="OO328_002635"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5931152.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4517..6751)
                     /locus_tag="OO328_002636"
     CDS             complement(4517..6751)
                     /locus_tag="OO328_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5931153.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6799..8100)
                     /gene="rlmD"
                     /locus_tag="OO328_002637"
     CDS             complement(6799..8100)
                     /gene="rlmD"
                     /locus_tag="OO328_002637"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5931154.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8157..10913
                     /gene="barA"
                     /locus_tag="OO328_002638"
     CDS             8157..10913
                     /gene="barA"
                     /locus_tag="OO328_002638"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5931155.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11145..12485)
                     /gene="gudD"
                     /locus_tag="OO328_002639"
     CDS             complement(11145..12485)
                     /gene="gudD"
                     /locus_tag="OO328_002639"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5931156.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12506..13846)
                     /locus_tag="OO328_002640"
     CDS             complement(12506..13846)
                     /locus_tag="OO328_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5931157.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13848..15200)
                     /gene="gudP"
                     /locus_tag="OO328_002641"
     CDS             complement(13848..15200)
                     /gene="gudP"
                     /locus_tag="OO328_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5931158.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15634..16083)
                     /locus_tag="OO328_002642"
     CDS             complement(15634..16083)
                     /locus_tag="OO328_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5931159.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16101..16883)
                     /gene="truC"
                     /locus_tag="OO328_002643"
     CDS             complement(16101..16883)
                     /gene="truC"
                     /locus_tag="OO328_002643"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5931160.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16883..17212)
                     /locus_tag="OO328_002644"
     CDS             complement(16883..17212)
                     /locus_tag="OO328_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5931161.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17834..18379)
                     /gene="syd"
                     /locus_tag="OO328_002645"
     CDS             complement(17834..18379)
                     /gene="syd"
                     /locus_tag="OO328_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5931162.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18447..19295
                     /gene="queF"
                     /locus_tag="OO328_002646"
     CDS             18447..19295
                     /gene="queF"
                     /locus_tag="OO328_002646"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5931163.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDKDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19407..20771
                     /gene="ppnN"
                     /locus_tag="OO328_002647"
                     /gene_synonym="ygdH"
     CDS             19407..20771
                     /gene="ppnN"
                     /locus_tag="OO328_002647"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5931164.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5068 a         5570 c         4976 g         5167 t
ORIGIN      
        1 cactgatcat tattactttg ctattttttg ggtatttgca gtgatgttct tccctttctg
       61 gttttttcat caaggcagta atttttgtcg cgcatgtaaa agtggagtct gtatttcagc
      121 aatccatctt ttagatttat tcctgttctc cgataaaact ttttccattg ctgtattttc
      181 cttctttttt actttcacca tatttatggt ctttacctgt ttatgtgtac ttcagaaaag
      241 agcatctggt agaagttatc attcagacaa cagcggttcc gctggaggct caagtggcgg
      301 cgcatccggc cgctggtaac tccaccataa aaaatgccag cccgaaggct ggcattatca
      361 aatcagataa agtcagactt atgcctggcc tttgatctct ttacgaccgt tgtacggtgc
      421 tttttcgccc agagcttctt cgatacgaat cagctggttg tatttagcaa cacggtcaga
      481 acggctcata gaaccagttt tgatctggcc tgcagcagta ccaacagcca gatcagcgat
      541 ggtagcatct tcagtttcac cagaacggtg agagataact gcagtgtagc cagcatcttt
      601 cgccatcttg attgcagcca gagtttcggt cagagaaccg atctggttga atttgatcag
      661 gatggagtta gcgatacctt tttcgatacc ttctttcagg atcttggtgt tggttacgaa
      721 caggtcgtca ccaaccagct ggattttgtc gcccagaact ttggtctggt atgcgaaacc
      781 gtcccagtca gattcgtcca gaccgtcttc gatagaaacg atcgggtact gtttggtcag
      841 ttcttccagg aagtgagtga attcttcaga ggtgaacgct ttgttgcctt cgccagccag
      901 aacgtattta ccatctttgt agaattcaga agctgcgcag tccatcgcca gagtgatgtc
      961 tttgcccagt tcataaccag cagctttaac agcttcagcg ataacagcca gagcttcagc
     1021 gttggaaccc aggttcggcg catagccacc ttcgtcacca acagcagtgt tcatgccttt
     1081 cgctttcaga acttttgcca ggtgatggaa aacttcagaa cccatgcgga tggcttcttt
     1141 cacagttttc gcgccaaccg gctgaatcat gaattcctgg atatcaacgt tgttgtcagc
     1201 gtgctcacca ccgttgatga tgttcatcat cggaaccggc atggagtatt tgcccggagt
     1261 gccgttcagt tcagcgatgt gctcgtacag cggcatacct ttagcagctg cagcagcttt
     1321 ggcgttagcc agagatacag ccaggattgc gttcgcgccg aatttggatt tgttttcggt
     1381 gccgtccagg tcgatcatga tcttgtcaat gccagcctga tctttagcat ctttgccaat
     1441 cagcgcctga gcgatcgggc cgtttaccgc agcaacagct ttggttacgc ctttacccag
     1501 gaaacgggat ttgtcgccat cgcgcagttc cagagcttca cgggaaccag tagaagcacc
     1561 tgacggagca gctgccatac cgacgaaacc accctccaga tgtacttcgg cttcaacagt
     1621 cgggttacca cgggagtcga tgatttcacg accgatgatt tttacgattt tggacattag
     1681 gttttcctca agtcactagt taaactgaaa ctccagacaa acaacgcgta cccaaggtac
     1741 gcgttgccgt tctaactttt ttacttactt cgcctgacgt ttctggaact cgctggcggc
     1801 tttcacaaag cctgcaaaca gcgggtgacc atcacgtgga gtagaagtaa actccggatg
     1861 gaactggcaa gccacgaacc acgggtgatt cggaacttcg atgatctcga ccaactgatc
     1921 atccccggaa cggcccgcaa cgcgcagacc tgcatcttca atctgtttca acagcatgtt
     1981 gttgacttcg taacggtgac gatgacgctc aacaattgtc ggcgcattgt atagctggcg
     2041 aaccaggcta tcgtcaacca actggcactg ctgtgcgccg agacgcatgg taccgccgag
     2101 atcgctcttc tcgctacgaa cttcaacgtt gccgttttca tcgcgccact cggtaatcag
     2161 cgccacaacc gggtacttac agtctggcac aaattctgta gagttggcgt tctccatgtt
     2221 ggcaacatgg cgagcgtaat caattaacgc cacctgcata cccaggcaaa tgcccagata
     2281 aggaatattg ttctcacgcg caaaacgcgc ggtcgtaatc atgccttcta cgccacgata
     2341 gccgaaaccg ccaggtacga ggattgcgtc cagaccttta aggatttcaa cgccgcgcgt
     2401 ttcaacatct tgtgaatcga tcagtttgat gttgacgctg acacgattct tcagcccacc
     2461 gtgtttcagt gcttcgatca ctgatttata agcatccggc agttcaatgt acttgccgac
     2521 cataccgatg gtgacttcac ttaccggatt cgcttcttcg aagataacct gttcccattc
     2581 ggacagattt gcttccgggc agtttaagct gaatcgttta caaatataat cgtccagccc
     2641 ctgagatttc aacaggcccg gaattttata gatggaatcg acgtctttca gagaaataac
     2701 cgctttttcc ggaacattac agaacaatgc aatcttcgca cgttcgttcg ccggtacagc
     2761 gcgatctgaa cgacaaatca ggatgtcagg ctggataccg atggagagca gctcttttac
     2821 agagtgctga gtcggtttgg ttttgacttc accagacgct gccatgtacg gcaccagcgt
     2881 caggtgcata aacagagtgt gctcacggcc aatttcaaca gccatctggc gaatagcttc
     2941 gaggaacggc aaggattcga tatcacctac tgtaccgccg atttctacca gtactacgtc
     3001 atgaccttcg ccaccttcca gcacgcgctc tttgattgcg ttagtgatgt gcggaataac
     3061 ctgcacggtt gcgccgaggt agtcaccgcg gcgttcttta cgcagaacgt cagagtagat
     3121 acgacccgtg gtgaagttgt tgcggcggct cattttggta cgaatgaaac gctcgtagtg
     3181 ccccaggtcc aagtcggttt cagcgccgtc ttcagtaacg aacacttccc cgtgttggat
     3241 tgggctcata gtacctggat cgacgttgat gtaaggatcc agtttcatga tggtcacatt
     3301 gaggccacgg gcttcaagaa tggctgcgag ggaggctgcg gcaatgcctt tacccagaga
     3361 ggatacgacc ccgccggtca caaaaatata gttcgttgtc atgctgaacc tgagaagtta
     3421 ggttgaaaag acgatggaat aaccaagacg ggaaagcagt atacccgaac atgacctgtg
     3481 ccacaaactt tcattatccc tcctcttcgc cagcgcacta ttgaaatcag gagtgagaaa
     3541 atagcccctt tggggtaaat gtttttgacg caaatcaagc gcttgtcatt taaaaaatga
     3601 cacaaatggc gcttgaccgc gtaattccct tagagatcaa tttcctgccg ttttacctgt
     3661 tgccagactt cttccattgt ttcgaggtca acacctgtca tttccagtcc acgcgcggca
     3721 acaatacgct ccacttcgcg aaaacgacgc tcgaattttt cgttcacttt ttgcaatgcg
     3781 atttctgctt tcgtccctaa atggcgagcc agattaaccg tggcaaacag caggtccccc
     3841 atttcctcct ccagtttagc ctggtcgaca acagcctgcc gcgcctcgta catcacctcg
     3901 tcgatctctt cgtagacttt atcaaccacc gggccaagcg tcgtccagtc gaagccaacg
     3961 ttggcgcaac gtttctggat tttttgcgcc cgcattaaag ccggtaaact acgaggaata
     4021 tcgtccagcg ccgaatgctg cgctttctgc gcgcgctctt cggttttgat ttgctcccaa
     4081 cgggcaagca cttcactact gttttcggca gaactatcag caaaaacatg cggatggcga
     4141 cgctctaatt tatcgctaat agcagcgcaa atatcattaa agtcaaagcg cccttcttcc
     4201 tgagccattt gcgcgtaaaa caccacctgg aatagcagat cgcccagttc accgcgaaga
     4261 tcgtcaaaat cttcacgggc gatggcgtcc agcacttcgt aggtttcttc aagggtgtaa
     4321 ggcgcaatgg tggcaaatgt ctgctcttta tcccacgggc agccgttttc cggatcgcgc
     4381 aggcgctgca taatagtgag caaacggtcg atttgattca ttgaattgtc ctgaaaattg
     4441 cgggtctgtc aggtgtaata ccttcgagta aaagatatcg ctggatacgg ctgacgccat
     4501 atccagctat gtaagactaa ctcccgtgca accgacgcgc gtcgataaca tccggcacct
     4561 ggttgagttt acccagcacg cgccccagca cttgcaggtt gtaaatctca atggtcatgt
     4621 cgatggtcgc cagttgctgt ttggtgtcgc tacggctggc aacgccaagc acgttcacct
     4681 tctcgttggc gagaatggtc gtgatatcac gtaacaaccc actacgatca ttagccacca
     4741 cgcggaccac cagcgaatat ccggcggagt agctctcacc ccataccgcg tcaacaatgc
     4801 gttctggcgc atgggagcgc agttccgcca gttgttcgca atcggcgcgg tgtactgaaa
     4861 taccgcgtcc ctgggtaatg aagccgacaa tctcatctcc aggaatcggc tggcagcagc
     4921 gcgcgatgtg gtgcatcagg ttaccaacac cttcaaccac cacacgaccg ttatctttac
     4981 tgcggttttg cggtgtgtag cttttttgct gaagctgttt cagcgcggcg gcgtcctgct
     5041 cttcggcact cggcttatta aattgcgatt gcaggaagtt caccatctga ttgagacgga
     5101 tatccccgcc accaatcgcc gccagcaact cgtcgacatc attgaagttg taacgcggca
     5161 gcagatgttt ttctgcttct ttcaggctga tccccagatg ttccagctcg tcgtcaagga
     5221 tttgccgccc agccagaatg tttttgtcac ggtcctgttt acggaaccag gcgtgaattt
     5281 tcgaacgccc acggctggtt gtgacgtaac cgaggtttgg gtttaaccag tcacggctgg
     5341 ggttcggctg tttctgggtg ataatttcaa tctggtcgcc catttgcagc tggtaggtga
     5401 acggcacaat gcgcccgcca atttttgccc cgatgcagcg gtgtccgaca tcactgtgga
     5461 tgtggtaagc gaagtccagc ggcgttgatc ccgcaggcaa atcaacgaca tcacctttcg
     5521 gcgtaaagac atacacccgg tcgtcaaaca cctgactacg tacttcgtcg agcatttcgc
     5581 cggaatcagc catctcttcc tgccacgcaa tcagtttacg cagccaggca atccggtctt
     5641 catgtcccga acgtgcgccg ccagctgccg cgccctcttt atatttccag tgcgcagcaa
     5701 cacccaactc cgcatcttca tgcatctgtt tggtgcggat ttggatctca acggtttttc
     5761 cacccggccc cagaaccacg gtatgaatag actgataacc gtttggtttc gggttagcga
     5821 cgtaatcgtc aaactcatcc ggcaggtggc gatagtgagt gtgcactatc cccagtgcgg
     5881 cataacaatc ctgtaaacgc tcggcgacaa tacgtaccgc acgcacatca aacagctcat
     5941 cgaaggcgag gttctttttc tgcattttgc gccagatgct gtagatgtgt ttcggacgac
     6001 catacacttc agctttaacg ccttcagctt tcatctcagc gcgcagatgg ccgacaaact
     6061 cttcgatata gtgttcgcgg tcgagacggc gttcatgcag cagttttgcg atgcgtttgt
     6121 attcggtcgg gtggagataa cggaagcagt aatcttccag ctcccatttc agttgcccaa
     6181 tcccaagacg gttcgccaac ggcgcgtaga tattggtgca ctcttttgcc gccagtacac
     6241 gttcatcttc cggcgcatct tttacttcgc gcagatgagc aatacgctcc gccagtttga
     6301 tgactacgca gcgaaaatca tcgaccatcg ccaataacat ccggcgaacg ttatcgacct
     6361 gttcggagga aacagaatca gtgtgcgtcg ctttcagctg gcggatcgcc gccatatcac
     6421 gcacgccgtg aataaggtta acaaccgact taccgacgct ctcacgcagc acatcttcgc
     6481 tgactacgtt ggcatcagcc agagggaaca gcagcgccgc ccgcagcgtg tcaatgtcca
     6541 tacttaatgt cgagaggatc tccaccatct caacaccacg ccacaataac agactggcat
     6601 ccggatgccc ctgcgtctgt tgcagacaat acgcccaggt ttcggctaag cactcacacg
     6661 acttctggct ggtaataccc agacttgcga tccatttttc cggatcaaat tcaccagcct
     6721 tattgatatg tgcacttctt accgcaacca tcgtcctctc ctttagggac cagacctgcc
     6781 gaaatcggca aatcgtaact atttaacgcg cgagaaaagt accatcgatt ccagatgtcc
     6841 cgtgtgtggg aacatatcca gcatcgccag tcgcgcaatg gtatatcctg cttttaataa
     6901 cgcttcgcta tcccgagcca gcgttgcagg gttacaggat acataaacta tacgaatagg
     6961 ttccagtttt ataatttgct gcataacacc tgcggcacct gctcgcgccg ggtccagcaa
     7021 cactttatcg aagccgtttt tcgcccacgg ctgctttgtg acatcttctt caagattttc
     7081 gtgataaaac gtcacattct gtaagccatt aagacgcgca ttctgctggc ctttttccac
     7141 cagcgcagga acaccttcta caccgaccac actggcagct tgtgtcgcca atggcagtgt
     7201 aaagttgccc ataccgcaga acagatccag tacgcgatct tcaggttgca catccagcca
     7261 ttccaacgca cgcgctacca ttttttggtt cacacccgca ttgacctgaa taaaatcgcg
     7321 cgggctaaaa gttaagcgca acccgtttga gtcataccag ggcatctcac cagagacggt
     7381 ttcgagtatc tcactatcgg gggcgagata cagatccagg ccttcagaat gcgaaaagcg
     7441 ttccagtttt tcgcgatctg ccgaacttag cggtgcggta tggcgcaaaa tcatcagcgt
     7501 gccgctggtt gcctgtacca gttcaacatg accaagatgg cgcatagctt gtaagctgcc
     7561 cagacatgcc ctgactttgg gcagcaatgc ttcaagttgg ggcgctaaaa tggggcattg
     7621 tttgacgtcg acaatgtcac tggagcccgc tttgcgaaac cccatctgaa gttgctgtgt
     7681 tttcggtaag tagttcagac ttaaacgcgc gcggcggcga tagccccagg gaacatcggc
     7741 gatcacttca gagacatcgt gtttcattaa tcgggcgagt gccgcacttt tgcttcgctg
     7801 ctgtaaatcc acgctggcgt gttgttgctg acagccgccg catacgccaa aatgaggaca
     7861 gcgtggcgtt tcgcgttccg ggctatcgct taaccggcgt acgactttag cgcgggcata
     7921 ctgtttttta tcttcagtaa cagcaacttc cgcgttttcc tgcggcaata atccggggat
     7981 aaataacgtt ttgccgttat gtcgcgccac gccctgacca aaagagtcga ggtcgttgac
     8041 tgaaacggtt atgatctgac gcgtcgtcgt gcgtcgtttt gcagagtaga attgcgccat
     8101 tggcgagact ttctcaattt aacagtgtga ccttaattgt cccataacgg aactccatga
     8161 ccaactacag cctgcgcgca cgcatgatga ttctgatcct ggcaccgacc gtccttattg
     8221 gtttattgct gagtatcttt ttcgtcgtgc atcgctataa cgacttgcag cgtcaactgg
     8281 aagatgccgg tgctagcatt attgagccgc ttgcagtttc tactgaatat ggcatgagcc
     8341 tgcaaaatcg cgaatctatc ggtcagttaa taagcgtact gcatcgtcgc cattccgata
     8401 ttgttcgcgc gatttcggtt tatgatgaaa ataaccgact ctttgtcacc tccaattttc
     8461 atcttgatcc ctcatcaatg cagctcggca gcaacgtgcc gtttcctcgc cagctcactg
     8521 tcactcgtga cggcgatatt atgatcctcc gcacgccgat tatttctgag agttactccc
     8581 ccgacgaatc gcccagtagc gatgccaaaa atagtcaaaa tatgttggga tatattgcgc
     8641 ttgagctgga tcttaaatcg gttcgcttgc agcaatataa agagatcttt atttcctgcg
     8701 tgatgatgct gttttgtatc ggtattgcgc ttatttttgg ctggcgctta atgcgcgatg
     8761 taaccggtcc gattcgcaac atggtgaata ccgtcgaccg catccgtcgc gggcaactcg
     8821 acagccgggt ggaaggattt atgctcggcg agctggatat gctgaaaaac ggtatcaact
     8881 cgatggcaat gtcgctggct gcttatcacg aagagatgca gcacaatatc gaccaggcga
     8941 cgtccgatct gcgtgaaacg ctggagcaga tggaaattca gaacgttgag ttggatctgg
     9001 cgaaaaagcg cgcccaggaa gcggcgcgta ttaaatccga gtttctggca aatatgtcac
     9061 acgagctgcg tacaccactg aatggtgtta ttggctttac ccgcctgacg ctgaaaacag
     9121 aattaacacc aacgcagcgc gatcacctga atacgattga acgttcggca aataatttgc
     9181 tggcaattat taatgatgtt ctcgacttct cgaaactgga agcaggtaag ctgattctgg
     9241 aaagtattcc attcccacta cgcagcacgc tggatgaagt cgttactctg ctggcacatt
     9301 cttctcacga taaagggttg gaactgacgc tcaatattaa aagcgacgtg cctgataacg
     9361 tgatcggcga cccactgcga ttacagcaaa tcatcactaa cctggtgggg aatgcaatta
     9421 aattcaccga gaatggcaac attgatattc tggtagaaaa acgtgcgctg agtaatacca
     9481 aagtgcagat tgaagtgcag attcgggata ccggcattgg tattcctgaa cgcgatcaat
     9541 cgcgcttatt ccaggccttc cgacaggctg atgccagtat ttcccgccgt catggtggca
     9601 ccgggctggg gctggtgatt acacaaaaac tggttaatga aatgggcggc gatatttcgt
     9661 tccatagcca gccgaatcgc ggttcaactt tctggttcca cattaatctc gatctgaacc
     9721 cgaacattat tatcgaaggg ccatccatcc agtgcctcgc aggtaagcgc ctggcctatg
     9781 tcgaaccaaa ctccgcagca gcgcaatgca cgctggatat tttaagtgaa acgccgctgg
     9841 aagtggttta tagcccaacg ttctccgcgc tgcctcccgc gcattacgac atgatgttgt
     9901 taggcatcgc ggtgaccttc cgcgagccgc taacaatgca acatgagcga ttagcgaaag
     9961 cggtatcgat gaccgatttc ctgatgctgg cacttccttg ccatgcacaa gttaatgctg
    10021 aaaaactcaa gcaagatggt atcggcgcgt gtctgctgaa accattaaca cctacgcgcc
    10081 tgttgcctgc cctgacggaa ttttgtcatc acaaacaaaa cacgcttttg cctgtaaccg
    10141 atgaaagtaa gctggcaatg acagtcatgg cggttgatga caaccccgct aacctgaaac
    10201 ttatcggcgc attgctggaa gatatggtgc aacatgtgga actttgcgat agcgggcatc
    10261 aggcagttga acgggcgaaa cagatgccgt tcgatttgat cttaatggat attcaaatgc
    10321 cggacatgga tggcattcgg gcctgcgaac tcatccacca actcccgcat cagcgacaaa
    10381 cgccggttat cgcggtaacg gcgcatgcaa tggccgggca aaaagagaag ctgcttggcg
    10441 cagggatgag cgattatctg gcgaaaccga ttgaagaaga gcgattgcat aatttgttgt
    10501 tgcgctacaa gcctggcagc ggtatttcgt ctcgcgtcgt gacgcccgaa gtcaacgaaa
    10561 ttgtggtgaa cccgaatgcg accctcgact ggcaactggc actacgccag gcagcaggaa
    10621 aaaccgattt agcgcgcgat atgctgcaaa tgttactcga tttcctgcct gaagttcgca
    10681 acaaagttga ggaacagctg gctggagaaa acccggaagg cctggttgat ttgattcata
    10741 aactgcatgg cagttgcggc tatagcggcg taccacgtat gaagaatctc tgccagctga
    10801 tcgaacaaca gctacgtagc ggtactaaag aagaagattt ggagccggag ctgctggaac
    10861 tgttggacga gatggataat gtcgcgcgcg aagccagcaa aattctcggg taacggacgt
    10921 cttgtcgagt agcaatcggc tttttatgac gctggtttca gattggtgac aaagtgcctg
    10981 tgtttatgcc ggatgcggcg ttaacgcctt atccggccta caaaaccaat taaattcaat
    11041 gaattaaaaa atgatgtaga cctgatacat agcacaacag gctattttgc gtttagcatc
    11101 agtctcaaac cggctccaga tagagccggt tttggttttc tgtcttaacg caccatgcac
    11161 gggcgcttgt tatcgaacgt ccagccagga atcagatact gcattcccat cgcatcgtca
    11221 cgcgcgccaa gcccgtgttt ctgatacagc tcatgggctt tcatcacttg atccatatcg
    11281 atttctacac ccagccccgg tttttctggc acctgtacca gcccgccttt gatctcaaac
    11341 ggttctttgg tcaggcgctg attaccttcc tgccagatcc agtgcgtatc aatagcagta
    11401 attttacccg gtgcagcggc ggcaacatgg gtaaacatcg ccagggaaat atcgaagtgg
    11461 ttgtttgagt gtgaacccca ggtcaggcca aattcatggc acatttgcgc cacacgtacc
    11521 gaaccttgca tagtccagaa atgcggatcc gccagcggga tatcaacgga ttgtaacgag
    11581 agcgtatggc ccatctgccg ccagtcggtg gcgatcatat tggttgcagt cggcaggcca
    11641 gtcgcgcgac ggaactctgc catcacttca cgcccggaga aaccttgctc cgcaccacac
    11701 ggatcttctg cataagccag cgaacctttc aggtatttac caattttaat tgcttcgttc
    11761 agcgaccagg caccgttagg atcgagcgta atacgcgcct gcgggaagcg ttgcgccagt
    11821 gccacaatag actcggcttc ttcttccccg gccagtacac cgcctttcag tttgaaatcg
    11881 ttgaagccat atttttcata cgccgcttcc gccaggcgca ccaccgcatc cggcgtcatc
    11941 gcttcttcat gacgcaggcg ataccagtcg catgagtcat ccggctggct ttgatacggc
    12001 agaggtgtgg ctttgcgatt accgacgaag aacagataac cgagcatttc gacttcgcta
    12061 cgctgttgac catcgcccag cagcgatgcc acgtttaccc ccagatgctg ccccagcaga
    12121 tccagcattg ccgcttctat cccggtaact acatgaatag tggtacgcaa atcaaatgtc
    12181 tgcaaaccgc gcccactagc atcacgatcg gcaaaagtat tacgcaccag cgtcagaacg
    12241 tttttgtatt cacccagcgt tttacctacc accagcggaa tcgcatcttc cagcgtttta
    12301 cggattttct cgccgccggg aatttcccct acgccagtgt gaccggaatt atctttgata
    12361 atcacaatat tacgcgtgaa gaacggtgcg tgtgcaccac tcagattcat cagcatactg
    12421 tcatgacccg ccaccgggat gacctgcatt tcagtaacaa caggcgtcgt aaattgagaa
    12481 ctcatattta tgtccttttt cagaatcaat gacggccgaa aacgggacgt ttacggtcaa
    12541 aggtccagcc ggggatcagg tactgcatcg gacctgcgtc gttacgcgca ccgcaaggca
    12601 gacgtttata ggcctcatgt gccttttgta cctgttccca gtccagttcc acgcccagac
    12661 cgggcgcatc aggaacggca atttttccgt ttttaatctc cagcggattt ttggtcaggc
    12721 gacaatcgcc ctcctgccaa atccagtggg tatcgatagc ggtaggatta cccggtgccg
    12781 ccgcgcccac atgggtaaat atcgccagag agatatcgaa atggttgttg gaatggcagc
    12841 cccaggtcag cccccagtcg tcgcaaagct gcgccacgcg gactgcaccg gaaagcgtcc
    12901 agaagtgcgg atcggcaagt ggaatatcta ccgcattgag catcaccgca tgacccattt
    12961 cgcgccagtt ggtggcgatc atgttagtcg cgacgggcaa gccggtcgcc cgtcgaaact
    13021 ccgccatcac ttcacgtccg gagaagccct gttctgcgcc gcatggatct tcggcatagg
    13081 taagaacatc attcagccct ttgcataaag aaatggcttc atcaagcagc catgcaccgt
    13141 tgggatcaac ggtaatccgc gcatccggga agcttttctt caatgcacga acagtgtcga
    13201 tttcttgctc gccaggtaac accccgccct taagtttgaa atctttaaag ccatagcgat
    13261 cctgtgaggc ttccgccaga cgcacaacgg cttcgctgtt catcgctttc tgatggcgca
    13321 actgatacca ctcatggttg cccggcgtat tttccagata aggaagatcg gttttggtcc
    13381 gatcacctat ataaaacaga taaccgagga cggtaatagt ctcgcgttgc ttgcctggcc
    13441 ccaacagttc gcagaccgga acattcagcg ccttacctag caggtcaagc aaggcggctt
    13501 ccagcgccgc cacggcatta acgcgcaatt caaaagtcca ggcacctttg ccaaaggtat
    13561 caaaatcggc tgcctgatta cctttatgta cctgctgaac cactttattc aggcgcgcaa
    13621 cttcctggcc cagaaccatc ggaatagcat cgaccagtgt ttgataaatc acctctccgc
    13681 ccggcgcttc accaatgccg gtatgcccgg cgttatcggt gagtaccacg atattgcggg
    13741 tgaagtatgc gttatgcgca ccaccaatat tgagcaacat gctgtcatgt ccggcgaccg
    13801 gaatgacttt catatcagta ataacaggac tggattgtgt cgccatctta ttgccccgcg
    13861 acaggtttca gctcgatacg cttgatatcg cccaccagca ccaggtagct cagtaccgcg
    13921 attagggcat gaacaccaac ataaatcagc gccccattaa acgagccagt cgtgccaacg
    13981 atataaccaa ttgcgattgg cgtgacgatg ccagaaatgt taccgaacat gttgaacagg
    14041 ccaccggaaa gaccgctgat ctcttttggc gcggtatctg ccattactgc ccaacccagc
    14101 gccccgatgc ccttaccgaa gaaggccagc gccataaagc cgatgatcat ccactcaacg
    14161 ttgacgtagt tgcagaacac catcaccatc gacagcaaca tgcccattac gatcggtgtt
    14221 ttacgcgcaa tgttcagcga tcccgtgcgg cgcatcagcc agtcggaaat aattccaccc
    14281 agcacaccgc cgataaaacc gcaaaccgcc ggaacggaag ccacaaagcc cgctttcaga
    14341 atcgacatcc cacgcgcctg caccagataa accggaaacc aggtaatgaa aaagtaagtc
    14401 agggcgttga tacagtactg accgatataa acgccgatca tcatccgtga cccgagcagc
    14461 tgtttgatct gcccccactt cacgctgaac ggcactttaa ctttggtgct ttgctgatcc
    14521 atattgatca gcgcgccacc cgcggcgata tactccagct ctttctgatt tacccccgga
    14581 tgttgatttg gctcgtggat gactttcaac cagataaagc tgatgacaat gcccagaccg
    14641 cccataaaga agaagacgtg tgaccagccc acttcatgcg tcagccagcc cataatcggc
    14701 gcgaagatca ccgttgcgaa gtattgagcg gagttaaaaa tcgacaccgc cgttcccctt
    14761 tcctgcaccg gaaaccaggc cgcaacaatg cgactgttgc cggggaaaga tggcgcttca
    14821 gcaagcccga ccaggaagcg cagagtaaac agggcaacga taatgccgaa tccactaaag
    14881 atatcgacga agccttgcag caaggtaaac atcgaccaga taaagatcga ccagaagtag
    14941 acgcgttttg aaccaaaacg atccagcaac caaccaccag ggatctgccc gataacataa
    15001 gcccatgaga aggcagagaa cacatagccc attcccacgg gatcaaggcc gatatctttg
    15061 gccatttccg aaccggcaat agaaagcgta gcgcggtcgc cgtagttgaa ggatgtgacg
    15121 ataaacaaca tcaccactat ccagtaacga gcattcgtgc gtttttccac actgctcgca
    15181 gcctgactta aagaactcat tgttgcactc ctgaaaattc gcgttagcca cgctcactct
    15241 ggactgcgac atcgccagga aatcagaggt gacgtagggt gttttttgcc gtttttatag
    15301 gtcgttcgcc gaatacggcg cgtgtttata tctggcaata gcagtataaa aagcgcgcca
    15361 tagcggctca ccgtgcaaca acacaacatt aatgcgttca atgaggcccg attttggcat
    15421 tagccctgga cggtggaatc cacttcacgg aaatgaaaac aagaacaaga aaggaagggt
    15481 aaaaacgaag aaataaagaa gagtatgaaa tggatcgctt gactctaggc aaacgccagt
    15541 aaaaatccgc gctatgaagc agtttttact ggcatttgcc tgaaaagatt cgattcagca
    15601 ccgctaaaac gacatttacc gctcgctgac atatcaggac aacagcgtgc cccactgttc
    15661 gacccacgga tttgattccg tttccggttc cgggttctca ctggcgtcaa tcaacagcat
    15721 ttcgccaacc cgctgagcgc tctgttcctg caacaaggca tcgaactgtt tgccgccatt
    15781 gcaaaaattt acataactgc tgtcgccgag cgcaatcacg ccataacgca gattcggctg
    15841 gaagcccaga ctatctttga ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc
    15901 ctgcccggtc gtggacgtaa ccaccagaac atacttatcc tgatagggca gccagtcgct
    15961 taattcagga tcttcaaata ccgttgcttt gtggccctgt gcggtcagaa ttgcttctgc
    16021 ttcttccgcc actaacagcg aatttccgta catggtgccg acaaaaatac caatttccgc
    16081 cataccgttt tctccctgga ttaggaactt atctctccat cctgacccga tggcgcactg
    16141 aactcaaccc tttcattttc aggaagcaga ccgcgccagc caaattgtga taacgcctgc
    16201 atccaggtgt cgtccaggcc cgcgtgaata gtcagcggct cgccagtaaa aggatgcgtc
    16261 aatgacaact gactggcatg tagcattaac cgctggaggc caaaatgctc agcaccgctg
    16321 cgattctggc gtaaatcgcc atgtttgcta tcgccaataa tcggatgacg caaatgggca
    16381 agatgtcgtc gaagctgatg tttgcgtccg gttttcggct ccagttccac caggccgtag
    16441 cgcgtggtcg ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag accgcgataa
    16501 tgcgtcactg ctggctgcgg gcctttatct tcgcgggcaa atttatcagc gattttgtcc
    16561 agttcttcca ccagtggata atccaacacc gcttcttcca tcaaccagcc gcgcacaatc
    16621 gcatggtaac gtttctggat ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc
    16681 tcgctggata atcccatcaa caacacacca gaagtgggtc ggtccagacg atgagcagta
    16741 aaaacatgct gtcctatctg gtcacgcacg gtttgcatga ccactacttt ctcgtcgcga
    16801 tccagccagc tacggtgaac cagccagccg gagggtttat ttaccgcaac cagccattca
    16861 tcctgataga gtatttccag cattagctcg catcatccgc aaaaagagca tccagttttt
    16921 ccaactcagc cagaataagc gcgcgttgcg gatggtccgt cgccagcgcc atttcataat
    16981 agggtgcaac agcaaaagcg cccggtaacg gctggttgtt atttaacaaa tcgtgcattc
    17041 gcgggatcag cccccactgc aaccactcca gtggttccat ggtgtccata aagaacggtt
    17101 gggtactatt aaattgatgc ggctggggtt catcgtttcg ccagtgctga tgttcacgca
    17161 gtaacgcttc aagcgcctgc aactggagac gaacgcggtc atgagtggtc ataaagcaac
    17221 ctcaataaga aaaactgccg cgaaggatag caggaataaa aaaagggagc actgtattca
    17281 cagtgctccc ggttcgtttc gcagcattcc agctactttt gttgctccct gctcatcctt
    17341 gacaactttt cctctggcct tgcggccaat cgttcatcct gaactattgc ttcctgctca
    17401 caccaccccg atgtgatact tcatcctgaa gtgtccctgg ccttcctgac ccaccgaatc
    17461 atcctgaccg gttctcattc tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc
    17521 tcttcgcttc atccagaagc ctttccctga aacaccatcc tggtgtgtcc tgcagaagtg
    17581 tcatcatcct gatgttcact tcgttgtctg actccctgtc gacgaagata gaatcgtctt
    17641 tttcgcgaag tcttacaagg cgcttacaga caatgcctta aggaaaattt catatgaaag
    17701 ttaagaatta acaatcaaca ttttgatttt aaatgaaaat aacgattctt atctttttaa
    17761 gtattcgctt atttcctggc gatctcctac agagtctgta agagatctct cacaaccaga
    17821 tggggtattt ggattacaga agaggcttaa gttgattaag gaattccgca agatttgagg
    17881 ctaaattcgt ccgtttacgt gttcccagcg tctctttgca cacttcaccg gagagattac
    17941 acaccgagat gacctctaac tcttcttcca gcgtcgcgat aaatagcgtg ggcggcagtt
    18001 ttagcctttt ctgagtcacc agatgaccaa tcagattttc ctgcacccga cggaagtcgt
    18061 cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc aaactgcgcg tgcatatccc
    18121 cggcaaactg agtcgtataa aaggtatgaa ttgtgggttg tatcacaata tcaaaagcgc
    18181 gttcaaccgc gtttacattt tgttccccgg taaacggctg gggttgccag tatacggcat
    18241 cttcggtagt tgaaataatg cacggtgaag gaacaccgta caattcctca cttaacggcc
    18301 agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct ttcagggctt
    18361 gtgcggtcaa atcgtccact aatttctctc ttcacttaaa ccagatacac ttgtcgttta
    18421 gtttatctgg tttatgacgg tgaaacatgt cttcttatgc aaaccatcag gcacttgcgg
    18481 gcctgactct tggaaaatca accgattacc gggataccta tgacgccagc ctactgcaag
    18541 gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa agcggataac ctgccttttc
    18601 acggtacgga tatctggacg ctgtatgaac tttcctggct gaatgcgaaa ggtttgccgc
    18661 aggtcgctgt cggtcatgtt gaacttgatt acaccagcgt aaatctgatt gagtcgaaga
    18721 gttttaagct ctatctcaac agttttaacc agacgcgttt taataactgg gatgaggtgc
    18781 gccagacgct ggagcgcgac ttaagcactt gcgctcaggg taagattagc gtggcgttat
    18841 atcgtcttga tgaactggaa ggccagccga taggtcattt caatggcact tgcattgatg
    18901 acaaggatat cactatcgat aactatgaat tcactactga ctatctggag aatgccacca
    18961 gcggtgaaaa agtagtggaa gagacgctcg tcagccacct gctgaaatca aactgcctga
    19021 tcacccatca accagattgg ggttcgatcc aaattcagta tcgtggacgc caaattgaca
    19081 gagaaaaact gctgcgttac ctggtctcat tccgtcatca caacgagttc cacgaacagt
    19141 gcgtggaacg catctttaat gacctgttac gcttctgcca gccagagaaa ttgagcgttt
    19201 acgcacgtta tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt aatagcgatt
    19261 ttgtcccatc gaccacaaga ctggttcggc aataaatttt ttctcaattt tgcgtgctgg
    19321 attcacgcag aaggttgtga aaggtcatca ggcagggcta ttgtaatcaa agggaatgac
    19381 gatattcgtc ccataaggag tttttcttga ttacacatat tagcccgctt ggctccatgg
    19441 atatgttgtc gcagctggaa gtggatatgc ttaaacgcac cgccagcagc gacctctatc
    19501 aactgtttcg caactgttca cttgccgtac tgaactccgg tagtttgacc gataacagca
    19561 aagaattgct gtctcgtttt gaaaatttcg atattaacgt cctgcgccgt gaacgcggcg
    19621 taaagctgga actgattaat cccccggaag aggcttttgt cgatgggcga attattcgcg
    19681 ctttgcaggc caacttgttc gcggttctgc gagacattct cttcgtttac gggcaaatcc
    19741 atcataccgt tcgttttccc aacctgaatc tcgacaactc cgtccacatc actaacctgg
    19801 tcttttccat cttgcgtaac gctcgcgcgc tgcatgtggg tgaagcgcca aatatggtgg
    19861 tctgctgggg cggtcactca attaacgaaa acgagtattt gtatgcccgt cgcgtcggga
    19921 accagctggg cctgcgtgag ctgaatatct gcaccggctg tggtccagga gcgatggaag
    19981 cgccgatgaa aggtgctgcg gtcggacacg cgcagcagcg ttacaaagac agtcgtttta
    20041 ttggtatgac agagccgtcg attatcgccg ctgaaccgcc taacccactg gtcaacgaat
    20101 tgatcatcat gccggatatc gaaaaacgtc tggaagcgtt tgtccgtatc gctcacggca
    20161 tcattatctt ccctggcggt gtgggtacgg cagaagagtt gctgtatttg ctgggaattt
    20221 taatgaaccc ggccaacaaa gatcaggttt taccattgat cctcaccggc ccgaaagaga
    20281 gcgccgacta cttccgcgta ctggacgagt ttgtcgtaca tacgctgggc gaaaacgcgc
    20341 gccgccatta ccgcatcatc attgatgacg ccgctgaagt cgctcgtcag atgaaaaaat
    20401 cgatgccgct ggtgaaagaa aatcgccgtg atacaggcga tgcctacagc tttaactggt
    20461 caatgcgcat tgcgccagat ttgcaaatgc cgtttgagcc gtctcacgag aatatggcta
    20521 atctgaagct ttacccggat caacctgttg aagtgctggc tgccgacctg cgccgtgcgt
    20581 tctccggtat tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt gaagagtttg
    20641 gtccttacaa aatcaacggc gataaagaga ttatgcgtcg tatggacgac ctgctacagg
    20701 gttttgttgc ccagcatcgt atgaagttgc caggctcagc ctacatccct tgctacgaaa
    20761 tctgcacgta atctcccctc c
//
LOCUS       DAJTRN010000069        20775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRN010000069 DAJTRN010000000
VERSION     DAJTRN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..579
                     /locus_tag="OO328_002648"
     CDS             <1..579
                     /locus_tag="OO328_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5931165.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO328_002649"
     CDS             597..1235
                     /locus_tag="OO328_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5931166.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO328_002650"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO328_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5931167.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO328_002651"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO328_002651"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5931168.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO328_002652"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO328_002652"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5931169.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO328_002653"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO328_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5931170.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO328_002654"
     CDS             complement(5552..6613)
                     /locus_tag="OO328_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5931171.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO328_002655"
     CDS             complement(6610..8007)
                     /locus_tag="OO328_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5931172.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNASFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO328_002656"
     CDS             8163..9161
                     /locus_tag="OO328_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5931173.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO328_002657"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO328_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO328_002658"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO328_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO328_002659"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO328_002659"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5931174.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO328_002660"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO328_002660"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5931175.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO328_002661"
     CDS             complement(14232..14855)
                     /locus_tag="OO328_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5931176.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO328_002662"
     CDS             complement(14852..15274)
                     /locus_tag="OO328_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5931177.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO328_002663"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO328_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO328_002664"
     CDS             complement(16089..17141)
                     /locus_tag="OO328_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5931178.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO328_002665"
     CDS             complement(17172..18014)
                     /locus_tag="OO328_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5931179.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO328_002666"
     CDS             complement(18001..18882)
                     /locus_tag="OO328_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5931180.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO328_002667"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO328_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20775)
                     /gene="ycjM"
                     /locus_tag="OO328_002668"
     CDS             complement(20200..>20775)
                     /gene="ycjM"
                     /locus_tag="OO328_002668"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5931181.1"
                     /translation="NWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAIL
                     LSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVY
                     HELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVN
                     ITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5666 c         5052 g         4986 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gacgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctagtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtt
//
LOCUS       DAJTRN010000070        20692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRN010000070 DAJTRN010000000
VERSION     DAJTRN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            82..351
                     /locus_tag="OO328_002669"
     CDS             82..351
                     /locus_tag="OO328_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5931182.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO328_002670"
     CDS             345..710
                     /locus_tag="OO328_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5931183.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO328_002671"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO328_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5931184.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO328_002672"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO328_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5931185.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO328_002673"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO328_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5931186.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO328_002674"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO328_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5931187.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO328_002675"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO328_002675"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5931188.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO328_002676"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO328_002676"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5931189.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO328_002677"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO328_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO328_002678"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO328_002678"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5931190.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO328_002679"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO328_002679"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5931191.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="OO328_002680"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="OO328_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5931192.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="OO328_002681"
     CDS             complement(16430..17188)
                     /locus_tag="OO328_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5931193.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO328_002682"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO328_002682"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5931194.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO328_002683"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO328_002683"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5931195.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..20101
                     /gene="glpD"
                     /locus_tag="OO328_002684"
     CDS             18596..20101
                     /gene="glpD"
                     /locus_tag="OO328_002684"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5931196.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRAHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20370..20600)
                     /locus_tag="OO328_002685"
                     /pseudo
     CDS             complement(20370..20600)
                     /locus_tag="OO328_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4890 a         5694 c         5217 g         4891 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg cgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    20101 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    20161 aactgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc
    20221 atggtcttgt aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt
    20281 gtcaaaaatc taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac
    20341 ccaacgtaac aaaactcagg cacgttcccc actcgccccg agaaaaatag cattaatgcg
    20401 cccagcgtca gcataaaaat attgagcaga aggtaaattg ttggcgtaaa gagtaaattg
    20461 caaacctcag gaatagcggc aagcccagcc cccagcatgg caataaccgc tgcaaaaaat
    20521 tgcgtcagta ttgggatgcc gaaaaaagtc attaccagcg aggtcaaaat ccattttcta
    20581 ttttgcatta ttctttccat tctttctgaa tggcgaaatt atactccccg agtccccttg
    20641 ccccttctgg acacttttcc gaaatgatgg cgaaaaaaac gggacccttt gg
//
LOCUS       DAJTRN010000071        20602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRN010000071 DAJTRN010000000
VERSION     DAJTRN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            86..385
                     /locus_tag="OO328_002686"
     CDS             86..385
                     /locus_tag="OO328_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5931197.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            395..919
                     /gene="ygaP"
                     /locus_tag="OO328_002687"
     CDS             395..919
                     /gene="ygaP"
                     /locus_tag="OO328_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5931198.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO328_002688"
     CDS             complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO328_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5931199.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2038..2487
                     /gene="alaE"
                     /locus_tag="OO328_002689"
     CDS             2038..2487
                     /gene="alaE"
                     /locus_tag="OO328_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5931200.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2524..2868)
                     /locus_tag="OO328_002690"
     CDS             complement(2524..2868)
                     /locus_tag="OO328_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5931201.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3020..3250
                     /locus_tag="OO328_002691"
     CDS             3020..3250
                     /locus_tag="OO328_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931202.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3597..3842
                     /gene="nrdH"
                     /locus_tag="OO328_002692"
     CDS             3597..3842
                     /gene="nrdH"
                     /locus_tag="OO328_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5931203.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3839..4249
                     /gene="nrdI"
                     /locus_tag="OO328_002693"
     CDS             3839..4249
                     /gene="nrdI"
                     /locus_tag="OO328_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5931204.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4222..6366
                     /gene="nrdE"
                     /locus_tag="OO328_002694"
     CDS             4222..6366
                     /gene="nrdE"
                     /locus_tag="OO328_002694"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5931205.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6376..7335
                     /gene="nrdF"
                     /locus_tag="OO328_002695"
     CDS             6376..7335
                     /gene="nrdF"
                     /locus_tag="OO328_002695"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5931206.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7690..8892
                     /gene="proV"
                     /locus_tag="OO328_002696"
     CDS             7690..8892
                     /gene="proV"
                     /locus_tag="OO328_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5931207.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8885..9949
                     /gene="proW"
                     /locus_tag="OO328_002697"
     CDS             8885..9949
                     /gene="proW"
                     /locus_tag="OO328_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5931208.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10007..10999
                     /gene="proX"
                     /locus_tag="OO328_002698"
     CDS             10007..10999
                     /gene="proX"
                     /locus_tag="OO328_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5931209.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11191..12370
                     /locus_tag="OO328_002699"
                     /pseudo
     CDS             11191..12370
                     /locus_tag="OO328_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO328_002700"
     CDS             12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO328_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5931210.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13221..13556
                     /gene="ygaH"
                     /locus_tag="OO328_002701"
     CDS             13221..13556
                     /gene="ygaH"
                     /locus_tag="OO328_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5931211.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13647..14177
                     /gene="emrR"
                     /locus_tag="OO328_002702"
     CDS             13647..14177
                     /gene="emrR"
                     /locus_tag="OO328_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5931212.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14304..15476
                     /gene="emrA"
                     /locus_tag="OO328_002703"
                     /pseudo
     CDS             14304..15476
                     /gene="emrA"
                     /locus_tag="OO328_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15493..17031
                     /gene="emrB"
                     /locus_tag="OO328_002704"
     CDS             15493..17031
                     /gene="emrB"
                     /locus_tag="OO328_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5931213.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO328_002705"
     CDS             complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO328_002705"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5931214.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO328_002706"
     CDS             complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO328_002706"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5931215.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19389..19817)
                     /locus_tag="OO328_002707"
     CDS             complement(19389..19817)
                     /locus_tag="OO328_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5931216.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO328_002708"
     CDS             complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO328_002708"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5931217.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4972 a         5270 c         5146 g         5214 t
ORIGIN      
        1 tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact
       61 taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtaccga
      121 acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga ttctgtgcat
      181 gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagcgc
      241 ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca acagccaacg
      301 ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac
      361 cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca
      421 tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga
      481 atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc
      541 aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg
      601 caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact
      661 cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca
      721 acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taaacggcgt
      781 tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg
      841 gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc
      901 gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc
      961 agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg agcaattggc
     1021 ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc agtgaattta
     1081 tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc agagctatta
     1141 cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca ggtgctaatt
     1201 ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta
     1261 gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc
     1321 atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa
     1381 accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttattat
     1441 atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc tgttattttc
     1501 tgaaactctc agaataattt caagattata tattagccat tttgatgagt aatttaggtt
     1561 attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca aaaccgaagt
     1621 gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc tttctgctgt
     1681 taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac aaacaccgaa
     1741 ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat taaaggcaag
     1801 atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa tccacaatgt
     1861 acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat aaaaattttt
     1921 cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga tggcaatgag
     1981 atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg
     2041 ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac
     2101 tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct
     2161 ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg
     2221 taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat
     2281 ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta
     2341 gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg
     2401 atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc
     2461 agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg
     2521 tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact
     2581 aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg
     2641 ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc
     2701 ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata
     2761 gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa
     2821 accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg
     2881 ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt
     2941 ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg
     3001 aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt
     3061 gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc
     3121 ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcgttgct
     3181 gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc
     3241 cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc
     3301 tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga
     3361 taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa
     3421 tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat cactatttgc
     3481 tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     3541 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     3601 gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     3661 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag
     3721 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     3781 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     3841 gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt
     3901 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     3961 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     4021 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     4081 tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg
     4141 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     4201 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg
     4261 atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg tcgcattcag
     4321 ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt
     4381 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     4441 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     4501 accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg
     4561 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     4621 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg
     4681 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     4741 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     4801 gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat
     4861 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     4921 gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc gcgtcagggg
     4981 gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     5041 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg
     5101 gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccctatgac
     5161 gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa
     5221 ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     5281 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacagtaaac
     5341 cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     5401 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatt
     5461 tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga tttttcccgc
     5521 acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca tatccgcagc
     5581 gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat
     5641 ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc cctggatttc
     5701 accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggcg
     5761 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     5821 ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt
     5881 gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     5941 atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg gtctatctct
     6001 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     6061 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga aaatctggcg
     6121 ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg
     6181 actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact
     6241 cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     6301 atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     6361 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     6421 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     6481 gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga acaacaactg
     6541 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     6601 ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc gaatatcagc
     6661 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc
     6721 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct
     6781 cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     6841 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     6901 ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac
     6961 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt
     7021 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     7081 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     7141 tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     7201 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     7261 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa
     7321 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat
     7381 tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt
     7441 tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat
     7501 atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg cgatttgctc
     7561 gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg
     7621 gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat aaagaaatca
     7681 ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt tggtgagcat
     7741 ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa
     7801 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     7861 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     7921 attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgac
     7981 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     8041 atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat
     8101 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     8161 gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     8221 gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca
     8281 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     8341 attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg aattgccatt
     8401 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg
     8461 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa
     8521 gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt cggcccacgt
     8581 tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt
     8641 aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     8701 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     8761 gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa
     8821 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     8881 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca
     8941 atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg gtgctgactg
     9001 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     9061 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt
     9121 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     9181 attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca tcacctggca
     9241 gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat
     9301 cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc tgctgttctg
     9361 tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag cggcgaaaat
     9421 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     9481 cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct
     9541 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
     9601 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
     9661 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
     9721 ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg
     9781 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
     9841 cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
     9901 gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg
     9961 cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt
    10021 actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc
    10081 gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa ccttccagac
    10141 gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt
    10201 ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa
    10261 ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga aattttatcg
    10321 tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga aaaccgccga
    10381 ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga
    10441 taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg
    10501 tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg
    10561 gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt
    10621 ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt
    10681 ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc
    10741 gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg
    10801 gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga
    10861 tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg
    10921 ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc
    10981 gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc tcatccgccg
    11041 ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca aaccacatcg
    11101 cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt attgatttcc
    11161 agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg
    11221 gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc
    11281 cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt
    11341 attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt
    11401 gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc gggcggtatg
    11461 ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc attaaccggt
    11521 ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat
    11581 aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat attgctggca
    11641 cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct
    11701 tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca aatgaaatca
    11761 gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt catcagcgat
    11821 aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt cagcattctc
    11881 tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga tggtgacatt
    11941 ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt
    12001 gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac tttcatggct
    12061 ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc tggtgctgga
    12121 cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa tacatcctga
    12181 tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg
    12241 ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg
    12301 cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc
    12361 cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag
    12421 cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt
    12481 aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat
    12541 ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt
    12601 cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat
    12661 catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt
    12721 gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt atggcccgtc
    12781 actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat gggcgtttgg
    12841 cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata atcgccgctg
    12901 gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac
    12961 ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc
    13021 attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa
    13081 acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa cgctattttc
    13141 tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc
    13201 attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg
    13261 cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa
    13321 ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc
    13381 tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc
    13441 tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa
    13501 cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca
    13561 caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg
    13621 gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc
    13681 taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc
    13741 tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg
    13801 ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta
    13861 ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg
    13921 ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac gatcgccgct
    13981 gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc
    14041 agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg
    14101 agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc
    14161 tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat
    14221 gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag
    14281 ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac
    14341 cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa
    14401 ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg
    14461 atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga
    14521 cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg
    14581 acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc
    14641 gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt gaggtgcaaa
    14701 aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca
    14761 acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc
    14821 aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac
    14881 tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tagctggcgc
    14941 tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt gccgtgcaac
    15001 cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt
    15061 gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt cagccggtca
    15121 ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg
    15181 atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc ggtaactgga
    15241 tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat
    15301 acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc
    15361 aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg cgtgaaatca
    15421 gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca
    15481 gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg
    15541 attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc taacgtggcg
    15601 atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg ggtaatcact
    15661 tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc
    15721 ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc ttgggcgtgt
    15781 ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc
    15841 gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc
    15901 tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc
    15961 ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg
    16021 attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga aacccgcacc
    16081 gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg
    16141 cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga aattatcatt
    16201 cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat
    16261 aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt
    16321 atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag
    16381 gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt
    16441 ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg
    16501 gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa
    16561 ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggtc
    16621 tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg
    16681 gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata
    16741 accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta
    16801 aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg
    16861 caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct gattatttcc
    16921 gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg gctggtgtgg
    16981 tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta
    17041 agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactagat
    17101 gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac
    17161 ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta
    17221 agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac
    17281 gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag
    17341 actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt
    17401 accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct
    17461 ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc
    17521 gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg
    17581 atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat
    17641 tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca
    17701 tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt tcttttctgt
    17761 caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc
    17821 tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc
    17881 ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata
    17941 ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag
    18001 caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc
    18061 gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac
    18121 tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc
    18181 cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc
    18241 agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc
    18301 ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac
    18361 agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac
    18421 agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca
    18481 ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg
    18541 tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg
    18601 agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc
    18661 tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca
    18721 ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag
    18781 cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag
    18841 aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg
    18901 tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg
    18961 tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac
    19021 atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcagc
    19081 atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg
    19141 gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc
    19201 agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt
    19261 aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg
    19321 acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag
    19381 gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac tgcaacatag
    19441 cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat gcgcatccat
    19501 cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact
    19561 gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt tttacgcaat
    19621 ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct
    19681 gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac cagcacgact
    19741 tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc
    19801 gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct gcacgggccg
    19861 cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac
    19921 gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag
    19981 cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg
    20041 cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt
    20101 caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt
    20161 cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa
    20221 tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac tgaagaccgt
    20281 agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc aggattgtgc
    20341 catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa
    20401 aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa
    20461 attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg aaattttttg
    20521 gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
    20581 tctatccagc tgagctacgg at
//
LOCUS       DAJTRN010000072        20486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRN010000072 DAJTRN010000000
VERSION     DAJTRN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            165..1538
                     /gene="mpl"
                     /locus_tag="OO328_002709"
     CDS             165..1538
                     /gene="mpl"
                     /locus_tag="OO328_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5931218.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(1621..2172)
                     /locus_tag="OO328_002710"
     CDS             complement(1621..2172)
                     /locus_tag="OO328_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5931219.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            2266..3618
                     /gene="pmbA"
                     /locus_tag="OO328_002711"
     CDS             2266..3618
                     /gene="pmbA"
                     /locus_tag="OO328_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5931220.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            3801..4183
                     /gene="cybC"
                     /locus_tag="OO328_002712"
                     /pseudo
     CDS             3801..4183
                     /gene="cybC"
                     /locus_tag="OO328_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(4228..4692)
                     /gene="nrdG"
                     /locus_tag="OO328_002713"
     CDS             complement(4228..4692)
                     /gene="nrdG"
                     /locus_tag="OO328_002713"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5931221.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(4850..6988)
                     /gene="nrdD"
                     /locus_tag="OO328_002714"
     CDS             complement(4850..6988)
                     /gene="nrdD"
                     /locus_tag="OO328_002714"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5931222.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(7382..9037)
                     /gene="treC"
                     /locus_tag="OO328_002715"
     CDS             complement(7382..9037)
                     /gene="treC"
                     /locus_tag="OO328_002715"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5931223.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(9087..10508)
                     /gene="treB"
                     /locus_tag="OO328_002716"
     CDS             complement(9087..10508)
                     /gene="treB"
                     /locus_tag="OO328_002716"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5931224.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(10627..11574)
                     /gene="treR"
                     /locus_tag="OO328_002717"
     CDS             complement(10627..11574)
                     /gene="treR"
                     /locus_tag="OO328_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5931225.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            11759..11812
                     /gene="mgtL"
                     /locus_tag="OO328_002718"
     CDS             11759..11812
                     /gene="mgtL"
                     /locus_tag="OO328_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5931226.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            11953..14649
                     /gene="mgtA"
                     /locus_tag="OO328_002719"
     CDS             11953..14649
                     /gene="mgtA"
                     /locus_tag="OO328_002719"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5931227.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(14855..15241)
                     /gene="ridA"
                     /locus_tag="OO328_002720"
     CDS             complement(14855..15241)
                     /gene="ridA"
                     /locus_tag="OO328_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5931228.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(15314..15775)
                     /gene="pyrI"
                     /locus_tag="OO328_002721"
     CDS             complement(15314..15775)
                     /gene="pyrI"
                     /locus_tag="OO328_002721"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5931229.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(15788..16723)
                     /gene="pyrB"
                     /locus_tag="OO328_002722"
     CDS             complement(15788..16723)
                     /gene="pyrB"
                     /locus_tag="OO328_002722"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5931230.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(16727..16861)
                     /gene="pyrL"
                     /locus_tag="OO328_002723"
     CDS             complement(16727..16861)
                     /gene="pyrL"
                     /locus_tag="OO328_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5931231.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(17142..17537)
                     /locus_tag="OO328_002724"
     CDS             complement(17142..17537)
                     /locus_tag="OO328_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5931232.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(17669..18382)
                     /gene="bdcA"
                     /locus_tag="OO328_002725"
     CDS             complement(17669..18382)
                     /gene="bdcA"
                     /locus_tag="OO328_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5931233.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            18453..19046
                     /locus_tag="OO328_002726"
     CDS             18453..19046
                     /locus_tag="OO328_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5931234.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            19191..19643
                     /gene="tabA"
                     /locus_tag="OO328_002727"
     CDS             19191..19643
                     /gene="tabA"
                     /locus_tag="OO328_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5931235.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            19766..>20486
                     /locus_tag="OO328_002728"
     CDS             19766..>20486
                     /locus_tag="OO328_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5931236.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         4951 a         5059 c         5405 g         5071 t
ORIGIN      
        1 tgcaatgttt atggagtaaa gcgaccgcaa caatcttaac aaataatctc aatgttgcgc
       61 tcaggtgaat cgcgccagca aattgcggat tatcctgaaa tgcgtttctc acttgcccga
      121 catatgcgta aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc attcatattt
      181 taggaatttg tggcacattt atgggcggtc tggcgatgct ggcgcgccag ttaggccatg
      241 aagtaacggg ttcggacgcc aatgtgtatc cgccgatgag caccttactt gagaagcaag
      301 gcattgagct gattcagggt tacgatgcca gccagctcga gccgcagccg gatctggtga
      361 ttattggcaa cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg gaaaaaaaca
      421 tcccttatat gtcaggtcca cagtggctgc acgattttgt gctgcgcgac cgctgggtgc
      481 tggccgttgc cggtacacac ggcaaaacca ccaccgcggg aatggcgacc tggattctgg
      541 aacagtgtgg ttacaaaccg ggatttgtga tcggcggtgt gccggggaac tttgaggttt
      601 cggcgcgtct gggcgaaagc gacttctttg ttatcgaagc ggatgagtac gactgcgcct
      661 tcttcgacaa acgctctaaa tttgtccatt actgcccgcg tacgctgatc ctcaacaacc
      721 ttgagttcga tcacgccgat atctttgacg acctgaaagc gatccagaaa cagttccacc
      781 atctggtgcg tatcgttccg gggcagggcc gtattatctg gccggaaaat gacatcaacc
      841 tgaaacagac catggcgatg ggctgctgga gcgagcagga gctggtgggt gagcagggtc
      901 actggcaggc gaaaaagctg accaccgatg cttccgaatg ggaagttttg ctggatggcg
      961 aaaaagtggg cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac aatggcctga
     1021 tggcgattgc agcggctcgc catgttggtg tagcgccggc agatgccgct aacgcgctgg
     1081 gttcgtttat taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat ggcgtcacgg
     1141 tatatgacga ttttgcccat cacccgacgg cgattctggc aacgctggcg gcgctgcgtg
     1201 gcaaagttgg tggtacggcg cgcattattg ctgtgctgga accgcgctcg aataccatga
     1261 aaatggggat ctgcaaagac gatctggcac cttcattagg tcgtgctgat gaagtcttcc
     1321 tgctgcaacc ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc tgcgttcagc
     1381 ctgcacactg gagtggcgat gtggatacgc tggcagatat ggtggtgaaa accgctcagc
     1441 ctggcgacca tattctggtg atgagcaacg gcggttttgg tgggatccat cagaaactgc
     1501 tggatggtct ggcgaagaag gcggaagctg cgcagtaatt cggcttcagc ctgatatagc
     1561 attgccggat aaggcgttta cgccgcatcc ggcaaaccat actcacatca acaacgaaaa
     1621 ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg cggatttcgg
     1681 cggcttattc ccttctttct ctttcttcgc gttgcggatc agtgtacgca gttgctgacg
     1741 atcggcatcg ggccacagat tcaatacttc agcgatggcg tcatcacctt gatcgatcag
     1801 acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt gacggttttt
     1861 cagcttatcc agcgcctgac gaataggctc tacgtcgcgc tggcgcagca tcttaccgat
     1921 gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct caatagccgg
     1981 gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat caacaatttc
     2041 cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac tgacccagat
     2101 aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc agtcttcggg
     2161 ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt aagatgcgca
     2221 ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc acttgcaatg
     2281 aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt ttccactgcg
     2341 ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa gaccaccggc
     2401 attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag tgatggcgcg
     2461 ctggggatca ctgtttatca ccagaaccgt aaaggtagcg catcgtctac cgatttaagc
     2521 ccgcaggcca ttgcccgcac cgtacaggcg gcgctggata ttgcccgtta tacctcgcca
     2581 gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc gaatctcgac
     2641 ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc ccgcgcagaa
     2701 caggcggcat tgcaggcgga caaacgcatc accaataccg aaggtggcag ctttaacagc
     2761 cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta ctgctcaacg
     2821 cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga gcgtgattac
     2881 gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt cggggccgac
     2941 tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat gaaagcgccg
     3001 gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg ggcgatagcg
     3061 ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa acaaattctg
     3121 ccggactggc tgaccattga agagcatccg catctgctga aagggctggc gtcgacgcca
     3181 ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg catcctgact
     3241 cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac cggacatgcg
     3301 ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca aatgctcaaa
     3361 gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag tgccattacc
     3421 ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat tcagtatccc
     3481 gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat tgtcaccgtc
     3541 ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt gccggagatg
     3601 aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta aaaaattaca
     3661 caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag cgtactcgca
     3721 gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg atgaagtgta
     3781 aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca gtctcctcgt
     3841 tggtattcag ttctgcgtcg tttgccgctg atcttgaaga caatatggaa accctcaacg
     3901 acaatttaaa agtgatcgaa aaagcggata acgcggcgca agtcaaagac gcgttaacga
     3961 agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc gaagataaat
     4021 caccggacag cccggaaatg aaagatttcc gccacggttt cgacattctg gtcggtcaga
     4081 ttgacgacgc gctgaagctg gcaaatgaag gtaaaaagaa gcgcaggctg ctgcagagca
     4141 actgaaaacg acccgcaacg cctatcacca gaagtatcgt taattcctca tttccctgtt
     4201 gcctgcactc aggtaacagg gaacttatca tcgcaaatga tgcaccacct gattgctgct
     4261 gccgcgccag atcagggaag ggtcttttaa atcctgcaca aatttgccgt cgaccagcac
     4321 gttaatcaaa tcaacaacct gcatttgcgc agcgttgagt tcgtcgagtt tatagcctgt
     4381 ccacacccag atgtctttac ccggacactc ggcgcggatg cgttttacca gtttcagaat
     4441 atccggcacg ttttgcggat gcagcggatc gccgccggag agggaaatcc cctggcgttt
     4501 gatacgagtg tcattcagat ctttaatgat cttgtcttcc attgctttgg taaatggctg
     4561 accagaattt acccgccatg tgcttttgtt atagcaaccg gggcattcat gaacacaccc
     4621 ggagacaaac agggtgcagc gagtgccggg gccgttgacg atgtcgacag gatagtactg
     4681 atgataattc attatttcgc ctggcttatc agtccgcgtg taggctgcat ccggtaatca
     4741 atgcctgatg cgacgctgtc gcgtcttatc aggcctacag ccgttgccaa atgtaggccg
     4801 gataaggcgt ttacgccgca tccggcattt gcttagcgca gagtgaagat taacctatct
     4861 gcccattccc caaatgttta acgcggcgct taacttcttc ctgcttacca gcgttaaacg
     4921 gacgtgcatc cgggctacct aaatatccgc acacgcggcg agttaccgac acacgggagg
     4981 cgtcatggtt accacatttc gggcaagtga agcctttgct ggtgcactcg aactcaccgg
     5041 taaagccaca ctcgtagcac tcatcaatcg gtgtattggt gccgtaatac ggtacatgct
     5101 gatagctgta atcccagaca tcttccagcg ctttcaggtt gtgctgaatg tttggatact
     5161 cgccgtagca aatgaaacca ccgttcgcca gcggcgggta aggcgcttca aagtcgatct
     5221 tgtcgtacgg gttcaccttc ttctccacat caaggtggaa actgttggtg tagtaacctt
     5281 tatcggtcac gcccggcacc acgccaaact cagcagtatc gagacggcag aagcggtcgc
     5341 acaggttttc actcggcgtg ctgtaaagac tgaaaccata acccgtttct tctttccact
     5401 gatccactgc ctgacgcaga cgttcaacaa tggcgatacc tttcgcgcga agcttctcgt
     5461 tgtcgtaaac atgctcgccg ctgaacagcg cgttaatggt ttcgtggatg ccgatgtaac
     5521 ccagcgaaat agacgcacga ccgtttttga agatttcaga aacatcatcg tcagcattca
     5581 gacgtacgcc acaagcacct tccatataga ggatcggggc gacgcgcgct ttcacgcctt
     5641 cgagacgagc gatacgggtc atcagtgcct tacgtgccag caccagacgt tcatccagca
     5701 gcttccagaa ggtggcttca tcgccttttg cttccagagc aatacgcggc aggttcaggc
     5761 tgatcacgcc gaggttgtta cgaccatcgt ggatctgctc gccgttttca ttttcccaca
     5821 cgccgaggaa gctgcggcag cccatcgggg ttttaaacga accggtgacg ttcactacct
     5881 gatcgtagtt caggatatcc ggatacatgc gcttgcttgc gcactccagc gccagctgtt
     5941 tgatgtcgta gttcggatcg ccttttttat ggttcaggcc atcgcgaatc gcaaacacca
     6001 gtttcgggaa caccgcagtt ttacggtttt taccgaggcc tgcgatacgg ttacgcagga
     6061 tcgattcctg aatcaggcgc gattcccagc tggtgcccag accaaaacca aaggttacaa
     6121 acggcgtctg accgttggcg gtgtgcaggg tgtttacttc gtactccagc gactggaagg
     6181 catcgtagca ctctttgatg gtacgagagt tagcgtagcc ttcggcgtcc gggatgttcc
     6241 actcttctgc ggtcttgcga tgtttgttgt agctggcggt aacaaacggc gccagcactt
     6301 catcgatacg gttaatggtg gtgccgccat aaatatggct ggcgacctga gcaataatct
     6361 gcgcagttac cgcggttgcc gtagagatcg acttcggcgg ttcaatctcg gcgttcccca
     6421 ttttaaaacc ctgggtcagc atgcctttca ggtcgatcaa catgcagttg aacatcggga
     6481 agaacggcga gtaatcgaga tcgtgatagt gaatatcgcc acgctcatgt gcctgcacca
     6541 cgtcacgcgg cagcaggtgc tggcgtgcat agtgtttagc cacgatcccg gccagcaggt
     6601 cgcgctgggt tggaatcacc ttgctgtctt tgttggcgtt ttcgttgagt aacgaggcgt
     6661 tggtctgctc gaccagacca cggatctcct ggttcaggcg accgcgtttt tcacgttcaa
     6721 tgtcgcgatc gtgacggtac tcgatgtaag cacgagccag ttgtttgtat ggacccgaca
     6781 tcagctggtt ttcaactgcg gtctggatct cgttgatatc cacctggttg cgcccctgca
     6841 tctgctcgtt gacaaccgcg gcaacagtgg cgcaataatc ggcatcatcg acttccgctg
     6901 ctttagctgc acgcagaatc gcttctttga tgcgctctga tttaaacggc actttgcagc
     6961 cgtctcgttt catcacatgc ggtgtcatga tcgctccata tttttaagaa caggttatcc
     7021 acagaaattg ggaaaggcgt tttccggttt tttcgtttcg ttttccggtg ctttccgcaa
     7081 tcgccatccg cttatccaca acgccgcacc tttttcgtgg ggagctgtag tagcaattat
     7141 agtcgattaa tacaacatat tgggttggga cgcattttaa agtctatata tagtgctttg
     7201 catcaaggat gttttaactt tttttgatgt agctcaaagt aaaaagcaga gcgtacggat
     7261 gacgggcgct acagcgttat gtaaattttt taatgaattt gctggttgaa aaatcaacaa
     7321 aaacaacatg ctgacagaca aaaccccgga atgaccggca ttatcggggc ttagggaaga
     7381 tttacttctg taaccaccag acagcctcaa aaggccgtaa attcatggca cagggttgtg
     7441 gtgaggcttc ttcgtagtta tgcatcacaa gctgccagtt gccgcgcatt tgccctggct
     7501 gccagggttg gatctcacgg ctaaggttgg caatgaccag caaggtttgc cccttccatt
     7561 cacggcgata gcaccacaat acagggctgt ttggcagcag atcctggtaa ttgccccatg
     7621 tcaggatggc ttcttgctta cgcagtgcga ttaacttttg gtaggtgtaa aacacagagg
     7681 agtcatcggc cagcgcggct tctacgttga tttgttgata gttatcgcct aggccaatcc
     7741 acggttcgcc agccgtaaac ccggcattat cgccgttgct ccattgcatg ggcgtgcggc
     7801 tgttgtcacg ggatttactg gcgaggattg ccaataactc gtcggcatca cgaccatcgt
     7861 tgcgcagctc ggcaaacata ttgaggctct ccacgtcgcg atagtcagta atgcgcgtga
     7921 aatgcgggtt ggtcatgcca atctcttcgc cctggtagat atacggcgtt ccctgcatgc
     7981 catgcagcac catcgccagc atttttgccg caggcacgcg gtattcacct tcatcaccaa
     8041 agcgagaaac aatgcgcggc tgatcgtggt tacaccagaa caaggcattc catgccacgt
     8101 tgtgcattcc ttgttgccag tggcggaaca atgttttcaa cgccacaaag tcaggtttag
     8161 ccagcgtcca tttttcacca ccgggataat cgaccttcag gtgatgaaaa ttaaaggtca
     8221 tcgacaattc actgcctgtc agtgccgcgt atcgctggca atgctcaagg ctggtggagg
     8281 acatttcacc tacggtcatt aacccgcgtg gcgtaaacac atcgcggttc atctcgtgca
     8341 aaaactcgtg tgctcgtggc ccgtcggtgt agaagcgacg cccgtcgcca tccaggtctt
     8401 cagggaaacg cgggtctttg gagatcagat tcaccacatc caggcgcaac ccgtcgaccc
     8461 cacgatcggc ccagaattca cagacttttt tcagctctgc gcgtaccgct ggattctccc
     8521 agttgagatc cgcctgttct ggtgcaaaga gatgcaaata gtactgttcg ctttccgcat
     8581 gccagcgcca cgcactaccg ccaaattttg aacgccagtt gttcggtggc gtttctggtt
     8641 cgccatcgcg ccagatataa aactggcggt aagggctttc tttgttcagc gcctcgcgaa
     8701 accaggcatg ttgggtagag gtatggttaa acaccatatc gagaatgata cgaatcccgc
     8761 gcgattttgc ctgcgtcacc agttcgtcaa aatcgtccag cgtgccgtag gtgggatcaa
     8821 tcgccgtata gttcgctaca tcgtaaccgt tatcgacctg gggagagaca taaaaggggg
     8881 ttagccagat ggcatcaacg cccagtttat gcagatagtc caggcgttgg ataacgccac
     8941 gtaaatcgcc ggtgccgcta cccgtggtgt cctgaaaact ctttggataa atctggtaga
     9001 taacgccgtt ttgccaccag tggggaagat tagtcattac gttattcctg caaatgcgag
     9061 ggggcgcaat tgcgccccga agaaaattaa acaatgtcca gcgtgcccag gcggtatttc
     9121 cgctgataga taaacgaggt gagtacaatc gggatgatga tggcgatagc cattgccagc
     9181 gcaaatacct gccagtagct cggttgaata gagagaattc ccggcaggcc ccctacgccg
     9241 ataccattcg ccataacgcc gttcaggccg catagcaatc ctgccagacc agaaccaatc
     9301 atcgcgcaca gcatcgggaa gcgatatttc aggttgatgc cgtacattgc aggctcagtg
     9361 accccaagcc aggcggagat agcggcaggc acggagatct cgcgttcatt gtgcttgcgg
     9421 ctggaaatga taatgcctat cacggcggag ccctgagcga tattcgacag cgcaatcagc
     9481 ggccacactg gcgtgccacc catgctttga atcatctgca aatcaatagc aagggtggtc
     9541 tggtgtacgc cggtgatcac cagcggcgcg tacaggaagc caaacaatgc cgcaccaatc
     9601 ggagcaaagc tgccggtcat caggtgacgt accgcaaagg caacgccatc gccaatcatg
     9661 cgaccaaacg gaccaatcag cgcatgggcg aggaacaccg cgaggatcag cgaacagacg
     9721 ggcaccacca ccagatagag gtaatccggt acgatgcgtt taaggcgagt ttcaataacg
     9781 cccagcgcca gcccggctaa cagtgccggg atcacctgcg cctgatagcc cactttggcg
     9841 atgctgaaca tgccaaagtc ccacacttcc ggcagctgct gcccgagcag ataagcgttc
     9901 atcagctggg gggaaaccag tgtcacgcca agcacgatac caaggatcgg cgtgccgccc
     9961 atttttttca ccgctgacca gcaaatcccg accggcaggt agaagaaaat cgcttcaccg
    10021 atcaaccaca gaaaatcgta gatcgtttgc agggaagggt acatttgcgc cagcgtctga
    10081 ccgttgctca tgggcaaatc gccgatcaca ttgcgaaaac cgaggatcaa accgccgcta
    10141 atcaacgcgg gcagcaacgg gaagaagatc tccgcgaaat gagagatcaa ctgctcatgc
    10201 catttcatat tttgccgggc ggcttttttt acctgctctt tatcaacctg cgcctgtccg
    10261 gttgacgcta ttagtgcttg atagtaatca cccacgttgg tgccaatcac cacctgaaat
    10321 tgcccggcat tggtgaaaca gcctttcacc atggggagtt gctcaatttc tttcggtctg
    10381 gcattggccg gttggttgag gacaaagcgc aggcgagtaa tacagtggct caccgtcgca
    10441 atattgccgc gcccgccgac cagttcaatc aaccgatcga tatccgtttg gtttattttg
    10501 ctcatcataa agccccatgg cagatgacat ttttggttgg ctgcagaata tattgcgcgg
    10561 aaaaatttaa aaacgggaac gttcccgaaa cgcagcgaag atcacaattt atcgttcagg
    10621 aaacgatcag gacagggtgg cggggatgat gatttgttgc ggttcgctgc gcccggttac
    10681 ctgtgcgatc aattggcagg ccgcctggcg tccagcttcg gcgtagccag gatctacggt
    10741 gacgatctcc ggatgaagga atttcattaa cggcgtatta cctacgctcg ccagttgcaa
    10801 ggtgtcgatg cgttgctctt gcaggtattt acttgcgcca agagccagcg tgtcggttgc
    10861 gcacagtaac gcggtagttt caggcgtaat cacttttgct acgttttcat agccttgctt
    10921 catagcaagc cctggcagag cggcaacggg atgcagttta tgcgctttgc agaacgccag
    10981 gtaggcttcg tgacgtcgct tcccggtcgt cacgtcacta tgcggcacgc cgagataact
    11041 gatattgcga tgcccctggt catagagccg ttgcatcaga atcttgattg ccccttcgtc
    11101 gtcataacag accgaagcaa agccttttgc gtcacgcgcc agcagaacca gcgatgactg
    11161 ccagtgggct aacatttctt cggttatgcc ggtaaaaccg aacagcacta cgccgtcgat
    11221 attacgccgt ttcagcaccc ccaaatgttc ggcaactaat tgtggggaaa actgattttc
    11281 catcatgatt ggatcgtaac cttgttcata gaacgctggc agcatggttt gaacggcgag
    11341 attttctgac aacgaatcca gacgggtaac aatgatggcg accactttat cgctttgccc
    11401 acgcatagcg cgcgcagagc gggaagggga aaatccatgc tgattcatca ctgcttcaac
    11461 acgctcgcgc gtgcgctgac tcacgccgct ttcgttattc agcacccggg aaaccgtaga
    11521 tttccccacg ccgcttaagc gcgcgatgtc tttgatggtc agccgatttt gcatcctgtt
    11581 gtcccataac gtgttgttta attatttgag cctaacttta cccgtgcatt cagcaatggg
    11641 taaagtctgg tttatcgttg gtttagttgc cagcaggtat tatatcgcca taaatgctac
    11701 gaatattatt ggattctcct tattatttgc ggcgcttttt tcacttaccg gaggttatat
    11761 ggaacctgat cccacgcctc tccctcgacg gagattaaaa cttttccggt aagcccgtct
    11821 tttcacggcg ttaccggatg cgtaaggccg tgacgtttta atgtccctgc tcagctttat
    11881 taccttcagg taaggcttcg ccacgcctga agacatttct gtactgtttc agacagtgcg
    11941 gagggactcc ttatgtttaa agaaattttt acccggttca ttcgccattt accttcccgt
    12001 ctggttcatc gcgatccatt gcctggcgcg cagcagacag tgaatacggc ggtcccgccg
    12061 tccttaagtg cgcattgcct gaaaatggcg gtgatgcccg aagaagaatt gtggaaaacg
    12121 ttcgacaccc atccggaagg attaaatcag gcggaagtgg aatctgcccg cgaacaacat
    12181 ggtgataata aattacccgc ccaacaaccg tcgccgtggt gggtacattt atgggtctgc
    12241 tatcgcaacc cctttaatat tttgctcacc attcttggtg ctatttctta cgccacggaa
    12301 gatttatttg ccgcaggtgt tatcgcgcta atggtcgcta tttctacgtt gctgaacttt
    12361 attcaggaag ctcgttccac caaagcggca gatgccctga aagcgatggt cagtaatacc
    12421 gcgacggtac tgcgagtaat taacgacaaa ggcgaaaatg gctggctgga gatcccgata
    12481 gaccagctgg tgcccggcga tattataaaa ctggcggcgg gggatatgat cccggcagat
    12541 ttacgtatct tgcaggcgcg ggatctgttc gtcgctcagg cgtcgttaac cggtgagtct
    12601 ctgcccgtgg aaaaagccgc tactactcgc cagccggagc acagcaatcc gctggagtgc
    12661 gacaccctgt gttttatggg caccaccgtg gtgagcggca cggcacaagc aatggtgatt
    12721 gctacaggag ccaacacctg gtttggtcaa ctggcggggc gtgttagtga gcaggaaagc
    12781 gagccgaatg cctttcagca agggatcagc cgcgttagta tgctgctgat tcgctttatg
    12841 ctggtgatgg ctccggtggt gttgcttatc aacggttaca ccaaaggcga ctggtgggaa
    12901 gcggcactgt ttgcgctttc ggtcgcggta ggcttaacgc cggaaatgtt gccgatgatt
    12961 gttacctcga cgctggcgcg cggcgcagta aagctgtcga aacagaaagt gatcgtcaaa
    13021 catctggatg ccattcagaa ctttggcgca atggatattc tgtgcactga taaaaccggc
    13081 accctgacgc aggacaaaat tgtgctggag aatcataccg atatctccgg taaaaccagc
    13141 gaacgcgtgc tgcatagcgc gtggttgaac agtcattacc agaccggact taaaaatctg
    13201 cttgataccg cggtgctcga aggtacggat gaagagtcag cgcgttcgct ggccagccgt
    13261 tggcaaaaaa ttgatgagat tccgtttgat ttcgagcgtc gtcggatgtc ggtggtagtg
    13321 gcagaaaata ccgaacacca tcagctggtt tgcaaaggtg cattgcagga aatcctcaat
    13381 gtgtgttcgc aggtgcgtca caatggcgag attgtaccgc tcgatgacac catgctgcgt
    13441 aagattaagc gggttactga tacgctgaat cgtcaggggc tgcgcgtggt tgcggtggcg
    13501 acgaaatacc tgccagcgcg tgaaggagat taccagcggg cggatgaatc cgacctgatc
    13561 ctcgaaggat atattgcttt ccttgatccg ccaaaagaga caaccgctcc ggcactgaag
    13621 gcattaaaag cgagtggaat taccgtaaaa atccttactg gcgacagtga gttagttgca
    13681 gcgaaagtgt gccatgaagt ggggctggat gcgggagagg tggtcattgg tagtgatatt
    13741 gaaacgctat ctgacgatga actggcaaat ctcgcacagc gcaccacgct gtttgcccgc
    13801 ctgacgccga tgcataaaga acgcattgtg actttactga agcgcgaagg gcatgtggtt
    13861 ggctttatgg gcgatggtat taatgatgcg cccgccttac gcgctgcgga tatcggcatt
    13921 tctgtggacg gcgcggtaga tattgcccgt gaagcggctg atatcatcct gctggaaaaa
    13981 agcctgatgg tgctggaaga gggggttatt gagggacgtc gcactttcgc caacatgctg
    14041 aaatacatca aattgacggc gagttctaac ttcggtaatg tgttcagcgt gctggtggcg
    14101 agtgctttct tgcccttcct gccgatgttg ccgttacact tgcttattca gaacctgctg
    14161 tacgatgtgt cacaggtggc gatcccgttt gataacgtcg acgacgagca aattcaaaag
    14221 ccgcagcgtt ggaatccggc ggatctgggg cgctttatgg tcttcttcgg accgatcagc
    14281 tcgattttcg atattctgac gttttgcttg atgtggtggg tattccatgc caacacgccg
    14341 gaaacgcaaa cgctgttcca gtcgggctgg tttgtggtgg gattactgtc gcaaacgctg
    14401 attgtgcata tgatccgtac ccgccgtgtg ccgtttattc agagctgtgc atcgtggccg
    14461 ttaatgatca tgaccgtgat cgtgatgatt gtcgggatcg cattgccgtt ttcaccgctg
    14521 gccagttatc tgcagctgca ggcgctgccg ttaagctatt tcccgtggct ggttgcgatt
    14581 ctggcagggt atatgacgtt aacccagttg gtgaaagggt tctatagccg tcgttacggc
    14641 tggcagtaaa gaataaactg ggcatggtgg cccggattca gattgcagac aaagtgagct
    14701 ttgttcatgc cggatgcggc gtaaacgccc aatccggcct acaaattcaa taaattgcag
    14761 gaatcacgta ggcctgataa gcgtagcgca tcgggcgatt ttgcttttgt catcagactt
    14821 aatccgggca tgatagcccg gatttccatc aagattagcg acgaacagcg attgcttcga
    14881 tctcaatctt cacgtctttc ggcagacggg caacttcaac gcaagaacgt gccgggaaag
    14941 tggcgttgtg ttcggtgaag aaggcttcgt aagtggcgtt tacggttgcg aagtcgttca
    15001 gatctttcac gaacacagtg gttttaacga tgtcgcccac tttcaggcca gcggcttcga
    15061 cgattgcttt tacattatcc agcgactgac gtgcctgtgc agcgacgtct gccggtactt
    15121 caccagtttt cggatttacc gggatctgac cggaggtgat gatcatattg cccagatcaa
    15181 cgccctgtac gtaaggaccg atagctgccg gtgcattttc cgtcgcgata gttttgctca
    15241 tgatttctcc tttattacag cggtaaaaag tctggctcat tatagggagc cagactttta
    15301 ttaccaaccg caattaattg gccagcacca cattatggga aaactctttt tcacagtatt
    15361 tgcatttaag cgcgatgtca ttggcgcgtt ttcgcacggc aaagctggat gaaaccggtt
    15421 cggcatggct gatacagttg ctgttcgggc agaccagcac attgtcgatg cgctctggca
    15481 gacttgggcg cgatttaccc accacttcat agttgtcgat acggttaacc gtggcttgcg
    15541 gtgcatacaa tgccagttgg tctacttgct cttcactcaa aaaggtattt tcgattttga
    15601 tcagatcttt gcggcccatc tcgccagaag gcaaattcag accaatggtg atgcgctgat
    15661 ctgtttcggt caacttgaac agactcaaca gcttaaaacc gatctgggcg gggatatggt
    15721 caattaccgt gccgcgttta atagcttcaa cctgcaattt attatcgtgt gtcatctcta
    15781 tttcccctta cagtaccaga tcgcgattca gaaccagtgc cagtaacgcc tggcgagcga
    15841 aaatcccgtt gcctgcttgc tggaagtacc aggcgtgagg cgttttatcc acatccgtcg
    15901 caatctcatc aacacgcggc agcggatgca gcactttcat attggctttg gcgttgtgga
    15961 gatcgctggc gcgaagaaca aactgcgctt tcacgttggc gtactcggac gggtccagac
    16021 gctctttttg cacgcgggtc atgtacagga tgtctacttc cgccatcact tcttcaatag
    16081 agctgtgcag actccaggcg atcccttttt catcgagcat atccagaatg tattgcggca
    16141 ttgccagcgc atccggcgcg ataaagtaaa aacggttgcc gtcgaacttc gctagcgcct
    16201 gggtcaggga gtgaacggtg cggccatatt tcaggtcacc aaccattgcg acgtggagat
    16261 tgtccagacg cccctgggtt tcctgaatgg tgaataagtc cagcaaggtt tgcgtcggat
    16321 gttggttgga gccatcaccg gcattcagta ccggtacatt gccagaaaat tcggtggcta
    16381 ggcgtgccgc accttcctgc ggatgacgca tcactatcgc atcgacgtaa gtgctgataa
    16441 ccgaaatggt atcggccagc gtttcgcctt ttttacccag tgatgtattg gcgctgtcgg
    16501 agaagcccac cacgctggca cccaggcggt gcatggaagt ttcgaaagag aggcgggtac
    16561 gggtagaggc ttcgaagaaa cagctggcaa tgactttgtg cttcaacagc tctggttgcg
    16621 ggtttgcttt cagtttcgcc gctgtcgcca gcaccagatt aaggtcatcg cgactaaggt
    16681 cgtttatgga aatgatatgt ttctgatata gcggattagc catcttttat ctcctgacac
    16741 ctaggcaaaa aaaagcccct cgattgaggg gctgggaatg ggtgatcaac gggaagaaaa
    16801 acggcaggcc agcgtctttt ttcagacgcg gtaagacaaa atgtcgaaca cactgaacca
    16861 tacatcctcc cggcaaattg tccggcatta tactcatagc cagaagcggt gcaagcattt
    16921 gatgcaatat tttgtcagcg caaacggttt atttgaatta aaagtcaaaa tatatgcatt
    16981 tttattttct atgattctgg aggggaagtg tcgttcggta tcaataattg cagacatttc
    17041 ctgctttttt ctttttcatt ttcacgcaat cagaaatgca ttttattcca ttcatccgac
    17101 ttatagggcg gagggtgaaa acgaacggct aacactattg cttatagctc agggatacgc
    17161 gctatcactt taatttcaaa atcaaagcct gccagccagg taacacccac cgccgtccag
    17221 tttggataag gtggggcgct aaatatttca tttttcaccg tcatgatgtc ttcagattgt
    17281 ttttccggat cggtatgaaa gctcgtaaca tcaatgatat cgtcaaaagt gcatcccgca
    17341 gctgccaggg tcgcatgcaa attatcaaag gccagtctga cttgttgctg aaaatcgggt
    17401 tctggtgttc cgtcctctcg acttcctact tgcccggaaa caaacagcaa atcgccggaa
    17461 cgaatagccg cagaataacg atgctcagca tacagtgaat gtcggccagc agggaaaaca
    17521 gcggttcttt ctaccatttg gttatcctca agatttacga catgaacaga agatttatat
    17581 ttatcgggag ccgcttttag ggggacgacg ttagtaaaca aaacccagac atcatggata
    17641 atggctgggc ttaattgagc gtagtcggtt atgcgccaaa cgcgccatca atggtatgca
    17701 tcgcgccggt aacaaaactg gcttctggcc ctgctaacca tgcgaccata ccagcgacct
    17761 cttccggttg cccatgtctt ttgatggcca taaaactatg caacatatcg cgcattggcc
    17821 cgttggcggg attaacgtcg gtatcaattg gtcctggctg gacgacgttg atggtgatcc
    17881 ctcgcggtcc aaaatcacgg gccagcccgc gcgccatgcc ttgcagggca gatttactgg
    17941 cggcataagc agccatgcct gcaacaggca tacgatcgcc attcacggag ccgatgatta
    18001 agatgcgccc gccttcgggc atctgccggg cggcttcaac agaggcatga taaggagcat
    18061 gaatattgat tttgaaaagg cgatcaatat cgtcggcatt taattccagg gcatcgccaa
    18121 agacgccaat acctgcattt accaccagga tatccaatgc gccgctctta cgaacgacat
    18181 caataacagc gtctctgtca gcactatctg tgaatactgc tgtcgctcca gtctcttgtg
    18241 ccagacgttc agcggcatct ttcgaccccg cataggtgaa tcgtacattg gccccatcgg
    18301 tgacgaaacg acgtacgata gcggcaccga ttccacgact gccaccgagg atgagaactg
    18361 tcttaccggt aaaagcgccc ataaggactc cttgatttat tatgtaacat gcattacaaa
    18421 actgttttaa ctttctgtca acaggttttg taatggtcac taaaaaacaa tctcgcgttc
    18481 caggtcgtcc cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa gtgctttttc
    18541 accaaaaagg tttcgatgca gtaagtgtct ctgaagttac tgattatctt ggtattaacc
    18601 ccccgagtct ctacgcggct tttggcagta aggctgggtt atttagccgt gtactcaatg
    18661 aatatgtcgg tacggaagct attccgcttg ccgatattct tcgtgatgat cgtccggtag
    18721 gcgagtgcct ggttgaggta ttaaaagaag cggcgcgcag atatagccaa aacggcggct
    18781 gcgctggctg tatggttctt gaaggtattc atagtcatga tccacaagcg cgtgatattg
    18841 ccattcaata ttatcacgcc gcagaaacga ccatttatga ctatatcgcc aggcggcatc
    18901 cacaaagcgc acaatgtgtg actgatttta tgagtaccgt gatgtcaggg ctttctgcga
    18961 aggcacggga ggggcactcc atcgaacaac tctgtgcaac agctgccctg gcaggggaag
    19021 cgataaaaac tcttctcaag gagtgatgct ggccttgatc cgaaaggcgg gaacgcgcct
    19081 gccgataagt tgtgataaga caataattca cgcgttaagg ctggcggaat tgatcatctt
    19141 ttcgtataac gatagaaatg aaacattgtt ttaattaagg agtggaaaag atgatcatcg
    19201 gaaatattca taatcttcag ccgtggctac cccaggagtt acgccaggcg attgagcata
    19261 tcaaagcaca cgttacggca gaaacgccaa agggcaagca tgatatcgaa ggcaatcgtc
    19321 tgttttatct tatctcggaa gatatgaccg agccgtacga agctcgccgt gcggagtacc
    19381 atgcccgcta tcttgacatt cagattgtgt taaaaggtca ggaaggcatg accttcagca
    19441 cgcaacctgc aggcgcgccg gataccgact ggttagctga taaagacatc gcatttttgc
    19501 cggaaggcgt tgatgagaaa acagttatcc taaatgaagg tgattttgtt gtgttttatc
    19561 cgggggaagt gcataaaccg ctgtgcgcag tgggcgcgcc agcccaggtt cgcaaagcag
    19621 tagtgaagat gctgatggcg tgatgacttt tcgccgtaaa taactccagg tttacggcga
    19681 gtttgtgaaa agagcgtttt ttgatatttt tttgtgagta aaatttgtaa tgcttagacg
    19741 ttcttattaa ctcaaggagt tcgtcatggg caaaatatca gatttgaatt attctcaaca
    19801 cattacatta gccgacaatt ttaaacaaaa aaatgaagcc ttagatacct ggtatgtggg
    19861 aatgaatgat tttgcccgaa ttgccggggg gcagaatagc agaagcaata ttctttctcc
    19921 tagagcattt ttggagtttt tggcaaagat atttaccctg ggttatgtgg attttagcaa
    19981 acgctccaac gaagcgggta gaaatatgat ggctcatatt gagttctcat cttattctaa
    20041 agatactgat ggcaatgaaa aaatgaagtt ttacatgaat aatcctgaag gggagcgagc
    20101 ggatttatct aaggtgaaga tagaaattac acttgcctca gcttctacta aagggattcg
    20161 tgaaggacat acagtcatta tatttaaaca atctgatggt tcgactaatc gttatgaagg
    20221 gaagtctttt gaaagaaaag atgatagttc attacacctg attactaaca aggttctggc
    20281 gtgttaccaa cgcgaagcta acaagaaaat agcgcgtcta ttaaataatc atcagaagtt
    20341 aaataatata caggagttaa atgattcgca ggagttaaat aattcacaga agttaaataa
    20401 ttcgcaggag ttaaataact cgcaggtgag ttgtaaaggt tcagttgatt ctacgattac
    20461 ggatttatta gaaaaagcat tgaata
//
LOCUS       DAJTRN010000073        20440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRN010000073 DAJTRN010000000
VERSION     DAJTRN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="OO328_002729"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="OO328_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5931237.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="OO328_002730"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="OO328_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5931238.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="OO328_002731"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="OO328_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5931239.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO328_002732"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO328_002732"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5931240.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO328_002733"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO328_002733"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5931241.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="OO328_002734"
     CDS             4451..4816
                     /locus_tag="OO328_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5931242.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="OO328_002735"
     CDS             4966..5484
                     /locus_tag="OO328_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5931243.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNVFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="OO328_002736"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="OO328_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="OO328_002737"
     CDS             6715..7197
                     /locus_tag="OO328_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5931244.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="OO328_002738"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="OO328_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5931245.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="OO328_002739"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="OO328_002739"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5931246.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="OO328_002740"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="OO328_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5931247.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="OO328_002741"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="OO328_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5931248.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="OO328_002742"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="OO328_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5931249.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="OO328_002743"
     CDS             10941..11732
                     /locus_tag="OO328_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5931250.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="OO328_002744"
     CDS             11753..13039
                     /locus_tag="OO328_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5931251.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="OO328_002745"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="OO328_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5931252.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="OO328_002746"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="OO328_002746"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5931253.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="OO328_002747"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="OO328_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5931254.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="OO328_002748"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="OO328_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5931255.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="OO328_002749"
     CDS             complement(16987..17277)
                     /locus_tag="OO328_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5931256.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="OO328_002750"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="OO328_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5931257.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="OO328_002751"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="OO328_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5931258.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="OO328_002752"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="OO328_002752"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="OO328_002753"
     CDS             19139..20380
                     /locus_tag="OO328_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5931259.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5279 a         5346 c         4790 g         5025 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatgttttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca
//
LOCUS       DAJTRN010000074        20352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRN010000074 DAJTRN010000000
VERSION     DAJTRN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..444)
                     /locus_tag="OO328_002754"
     CDS             complement(<1..444)
                     /locus_tag="OO328_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5931260.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO328_002755"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO328_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5931261.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO328_002756"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO328_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5931262.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO328_002757"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO328_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5931263.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OO328_002758"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OO328_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5931264.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OO328_002759"
     CDS             complement(5214..6812)
                     /locus_tag="OO328_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5931265.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO328_002760"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO328_002760"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5931266.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OO328_002761"
     CDS             complement(8491..9069)
                     /locus_tag="OO328_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5931267.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO328_002762"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO328_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5931268.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OO328_002763"
     CDS             10508..10687
                     /locus_tag="OO328_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5931269.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OO328_002764"
     CDS             complement(10807..11166)
                     /locus_tag="OO328_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5931270.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OO328_002765"
     CDS             complement(11528..11872)
                     /locus_tag="OO328_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5931271.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OO328_002766"
     CDS             12004..13914
                     /locus_tag="OO328_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5931272.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OO328_002767"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OO328_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5931273.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OO328_002768"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OO328_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5931274.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="OO328_002769"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="OO328_002769"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5931275.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="OO328_002770"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="OO328_002770"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5931276.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO328_002771"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO328_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5931277.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO328_002772"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO328_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         5022 a         5090 c         5273 g         4967 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt ta
//
LOCUS       DAJTRN010000075        19919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRN010000075 DAJTRN010000000
VERSION     DAJTRN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..577)
                     /gene="aroD"
                     /locus_tag="OO328_002773"
     CDS             complement(<1..577)
                     /gene="aroD"
                     /locus_tag="OO328_002773"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5931278.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQ"
     gene            complement(608..1474)
                     /gene="ydiB"
                     /locus_tag="OO328_002774"
     CDS             complement(608..1474)
                     /gene="ydiB"
                     /locus_tag="OO328_002774"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5931279.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1486..2758)
                     /locus_tag="OO328_002775"
                     /pseudo
     CDS             complement(1486..2758)
                     /locus_tag="OO328_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2941..4146)
                     /locus_tag="OO328_002776"
     CDS             complement(2941..4146)
                     /locus_tag="OO328_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5931280.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4246..4602)
                     /locus_tag="OO328_002777"
     CDS             complement(4246..4602)
                     /locus_tag="OO328_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5931281.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4738..4845)
                     /gene="rprA"
                     /locus_tag="OO328_002778"
     ncRNA           complement(4738..4845)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO328_002778"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5031..6143)
                     /gene="ydiK"
                     /locus_tag="OO328_002779"
     CDS             complement(5031..6143)
                     /gene="ydiK"
                     /locus_tag="OO328_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5931282.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6532..9588
                     /locus_tag="OO328_002780"
     CDS             6532..9588
                     /locus_tag="OO328_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5931283.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9585..9995
                     /gene="menI"
                     /locus_tag="OO328_002781"
     CDS             9585..9995
                     /gene="menI"
                     /locus_tag="OO328_002781"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5931284.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10095..10283
                     /locus_tag="OO328_002782"
     CDS             10095..10283
                     /locus_tag="OO328_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5931285.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10831..11199
                     /gene="sufA"
                     /locus_tag="OO328_002783"
     CDS             10831..11199
                     /gene="sufA"
                     /locus_tag="OO328_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5931286.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11208..12695
                     /gene="sufB"
                     /locus_tag="OO328_002784"
     CDS             11208..12695
                     /gene="sufB"
                     /locus_tag="OO328_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5931287.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12705..13451
                     /gene="sufC"
                     /locus_tag="OO328_002785"
     CDS             12705..13451
                     /gene="sufC"
                     /locus_tag="OO328_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5931288.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13426..14697
                     /gene="sufD"
                     /locus_tag="OO328_002786"
     CDS             13426..14697
                     /gene="sufD"
                     /locus_tag="OO328_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5931289.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14694..15914
                     /gene="sufS"
                     /locus_tag="OO328_002787"
     CDS             14694..15914
                     /gene="sufS"
                     /locus_tag="OO328_002787"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5931290.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15927..16343
                     /gene="sufE"
                     /locus_tag="OO328_002788"
     CDS             15927..16343
                     /gene="sufE"
                     /locus_tag="OO328_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5931291.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16492..17496
                     /gene="ldtE"
                     /locus_tag="OO328_002789"
     CDS             16492..17496
                     /gene="ldtE"
                     /locus_tag="OO328_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5931292.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17559..17795)
                     /gene="lpp"
                     /locus_tag="OO328_002790"
     CDS             complement(17559..17795)
                     /gene="lpp"
                     /locus_tag="OO328_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5931293.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18106..19518)
                     /gene="pykF"
                     /locus_tag="OO328_002791"
     CDS             complement(18106..19518)
                     /gene="pykF"
                     /locus_tag="OO328_002791"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5931294.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5216 a         4794 c         5101 g         4808 t
ORIGIN      
        1 cctgcatctc cagggtcgcg gcaagcaacg tcagcacatc gctggtactt tgcggcatca
       61 gcgcaatctt aggaatatcg gcgtcgaagg actgcatttt gcgcagacgg gcaatgattt
      121 cttcggcttc cggcgtttta tggaagtcat ggttggacat gacaactttc acatcatgcg
      181 cgtgggcgta ggcgacggtt tctttgacct gatcatcgcc ggtaaataac tccagatcga
      241 tcatatcaac caggccgctg tcgatggctg cacgattgag agcaatataa gcctcggtgg
      301 aaatcgcctg ctcgccgcct tctttggcac tgcggaaggt aaacagcagc ggtttttctg
      361 gcatggtttc acggagaatt tttgccgccg ccatgacaga ctccacattg gagaggtcgg
      421 caaagtggtc cacacgccat tccagaatat caaagtccgc ttcacgatag gcgagagctt
      481 cggatttcac gcgggcgata tctttcgcca tcagcgagac gatgatttta ggtgcgcccg
      541 caccaatgac gagatctttt acagttacgg ttttcatttt ttaccctttc tgcacgcggt
      601 cagcctgtca ggcaccgaac cccatgacct gtttaacata ttccagaggg aaatctttgc
      661 cagtccataa cgtgaactgt tcagcccctt gccacaacaa catgccgtat ccatcaatcg
      721 ttttgcaacc agcttgttgt gcctgctgca ataacttcgt catatgcggg ttatacacgc
      781 attcagtgac cagcagtccc ggatgtaaca gactgatatc attaaccaat gattcattct
      841 caaggggttt catacctact tttgtgccat tggttaaaat gtcggcggaa gccagggctt
      901 cagcaaaggc ttgctgatcg gcgagatcgg tatccgtgac gacacaatcg gtgttttcgt
      961 taacccgctg cgcgaaggcg agggctttat cgaagaactc atcccgacgg ttaaagagtt
     1021 taatttcttt taaaccttca attgccccct gcgcgccaat cgccgttgag gcacctccgg
     1081 cccctaacag caccatcgtt ttgcctttga tatcaaaacc gctctcttta atggcgcgaa
     1141 tatggcccgt gccgtcggtg ttatagccac gcagatagcc atcatcatta acgatggtgt
     1201 tgatggcacc caccagtttg gctgctggcg ttaattcatc aacatattca cacgccagtt
     1261 gtttgttcgg catcgacaca ccagttccgc gcattttgag ggcttttaat ccttcaattg
     1321 ctccgggaaa gctatcgtta tccacttcga aggccatata ggtaaatggc aatcccgctt
     1381 tttctaaggc tttattctgc atttcaggcg ataaactgtg gcggatagga taagccatca
     1441 atccaatcaa ttcgtatttt gcggtaacat ccataattaa ctcctttaac ctctatgctt
     1501 aattgtgttt aaccgggtgc taaatttgcg ttcgccaaac cgcacatcat tctcaggaat
     1561 aataaaaacg cggtaatagc ggctaaaaac aataattgcg gtaatcaggg ccagcagcgc
     1621 gaaagtaaaa tcgagaacaa tgatatattg caggccgatg ttcgacagat aaccggtaat
     1681 cagcggaata acaaagttag ccagtccacc catcatcata taaatactgg tgactttggc
     1741 tttgcttttg gggaaaaact ctgacatgac cgaaacaccg agctgtaaaa tgccgccagc
     1801 tgctgagaaa ccgataacaa aggcgccggc attacacacc agtggagaag ggtacaggta
     1861 gataatggca gctgttattg ttgccagcgc agagttaaat acattagccc agatgggccg
     1921 gaccattttt ttcagtagtg cggcaaaaat aaagacacag accaacgagc ccatactgta
     1981 ataagagatg gtttttaatg cctcagcttc tgacatacca gcaaaagcca tcgcatattt
     2041 gggcatccac accacaatca cataaaaggt cgagaatgcg gctacaccga acagtaccga
     2101 tgaaacacct tccagccaga ctaaaggttt tgctgttcat ttgcggtaat tcattagcta
     2161 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2221 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2281 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2341 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2401 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2461 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2521 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2581 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgacagag
     2641 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2701 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     2761 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     2821 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     2881 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     2941 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3001 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3061 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3121 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3181 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3241 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3301 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3361 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3421 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3481 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3541 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3601 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3661 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3721 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     3781 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     3841 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     3901 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     3961 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4021 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4081 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4141 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4201 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4261 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4321 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4381 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4441 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4501 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4561 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4621 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4681 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4741 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     4801 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     4861 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     4921 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     4981 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5041 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5101 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5161 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5221 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5281 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5341 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5401 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5461 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5521 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5581 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5641 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5701 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     5761 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     5821 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     5881 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     5941 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6001 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6061 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6121 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6181 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6241 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6301 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6361 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6421 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6481 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6541 cagatttccc tggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag
     6601 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6661 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6721 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     6781 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     6841 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     6901 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     6961 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7021 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7081 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7141 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7201 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7261 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7321 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7381 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7441 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7501 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatat gcctgacaaa
     7561 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7621 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7681 attggctggc aggtgtgccg cgagctggcg ggggttgaac gtatctatgc gatgcgcaaa
     7741 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     7801 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     7861 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     7921 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     7981 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8041 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8101 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8161 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8221 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8281 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8341 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8401 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8461 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8521 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8581 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8641 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8701 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     8761 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     8821 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     8881 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     8941 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9001 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9061 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9121 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9181 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9241 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9301 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9361 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9421 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9481 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9541 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9601 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9661 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9721 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
     9781 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
     9841 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
     9901 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
     9961 gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10021 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10081 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10141 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10201 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctacttcg
    10261 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10321 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10381 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10441 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10501 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10561 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10621 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10681 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10741 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    10801 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    10861 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    10921 ctggtggcaa agcagccagg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    10981 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11041 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11101 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11161 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11221 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaactat aaagaaggat
    11281 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11341 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11401 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11461 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11521 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11581 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11641 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11701 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    11761 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    11821 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    11881 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    11941 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12001 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12061 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12121 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12181 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12241 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12301 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12361 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12421 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12481 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12541 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12601 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12661 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12721 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    12781 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    12841 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    12901 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    12961 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13021 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13081 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13141 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13201 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13261 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13321 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13381 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13441 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13501 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13561 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13621 gcgggagata tatccccaca gcagcgtgat gccttagcgt taacgttaga cgccgtgcgg
    13681 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13741 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    13801 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    13861 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    13921 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    13981 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14041 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14101 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14161 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
    14221 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14281 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14341 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14401 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14461 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14521 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14581 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14641 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14701 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    14761 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    14821 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    14881 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    14941 gttcggcgga agagctggtg tttgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15001 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15061 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15121 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15181 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15241 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15301 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15361 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15421 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15481 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15541 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15601 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15661 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15721 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    15781 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    15841 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    15901 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    15961 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16021 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16081 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16141 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16201 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16261 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16321 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16381 ccggatggtc aatgtttaca ctgaccggca caggagtgtt ttggtttcag gtgaacataa
    16441 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16501 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16561 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16621 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16681 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16741 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    16801 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    16861 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    16921 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    16981 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17041 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17101 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17161 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17221 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17281 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17341 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17401 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17461 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17521 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17581 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17641 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17701 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    17761 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    17821 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    17881 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    17941 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18001 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18061 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18121 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18181 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18241 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18301 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18361 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18421 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18481 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18541 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18601 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18661 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18721 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    18781 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    18841 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    18901 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    18961 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19021 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19081 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19141 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19201 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19261 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19321 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19381 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19441 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19501 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19561 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19621 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19681 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19741 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    19801 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    19861 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgag
//
LOCUS       DAJTRN010000076        19878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRN010000076 DAJTRN010000000
VERSION     DAJTRN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..3847)
                     /gene="mukB"
                     /locus_tag="OO328_002792"
     CDS             complement(<1..3847)
                     /gene="mukB"
                     /locus_tag="OO328_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5931295.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRET"
     gene            complement(3847..4551)
                     /gene="mukE"
                     /locus_tag="OO328_002793"
     CDS             complement(3847..4551)
                     /gene="mukE"
                     /locus_tag="OO328_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5931296.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4532..5854)
                     /gene="mukF"
                     /locus_tag="OO328_002794"
     CDS             complement(4532..5854)
                     /gene="mukF"
                     /locus_tag="OO328_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5931297.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(5851..6636)
                     /gene="cmoM"
                     /locus_tag="OO328_002795"
     CDS             complement(5851..6636)
                     /gene="cmoM"
                     /locus_tag="OO328_002795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5931298.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            6772..7551
                     /gene="elyC"
                     /locus_tag="OO328_002796"
     CDS             6772..7551
                     /gene="elyC"
                     /locus_tag="OO328_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5931299.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7528..8421)
                     /locus_tag="OO328_002797"
     CDS             complement(7528..8421)
                     /locus_tag="OO328_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5931300.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8575..9321)
                     /gene="kdsB"
                     /locus_tag="OO328_002798"
     CDS             complement(8575..9321)
                     /gene="kdsB"
                     /locus_tag="OO328_002798"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5931301.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9318..9500)
                     /gene="ycaR"
                     /locus_tag="OO328_002799"
     CDS             complement(9318..9500)
                     /gene="ycaR"
                     /locus_tag="OO328_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5931302.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9552..10784)
                     /locus_tag="OO328_002800"
     CDS             complement(9552..10784)
                     /locus_tag="OO328_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5931303.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(10821..11807)
                     /gene="lpxK"
                     /locus_tag="OO328_002801"
     CDS             complement(10821..11807)
                     /gene="lpxK"
                     /locus_tag="OO328_002801"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5931304.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(11804..13552)
                     /gene="msbA"
                     /locus_tag="OO328_002802"
     CDS             complement(11804..13552)
                     /gene="msbA"
                     /locus_tag="OO328_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5931305.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13589..15848)
                     /locus_tag="OO328_002803"
                     /pseudo
     CDS             complement(13589..15848)
                     /locus_tag="OO328_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16055..16339)
                     /gene="ihfB"
                     /locus_tag="OO328_002804"
     CDS             complement(16055..16339)
                     /gene="ihfB"
                     /locus_tag="OO328_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5931306.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16499..18172)
                     /gene="rpsA"
                     /locus_tag="OO328_002805"
     CDS             complement(16499..18172)
                     /gene="rpsA"
                     /locus_tag="OO328_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5931307.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18283..18966)
                     /gene="cmk"
                     /locus_tag="OO328_002806"
     CDS             complement(18283..18966)
                     /gene="cmk"
                     /locus_tag="OO328_002806"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5931308.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19139..19876)
                     /gene="ycaL"
                     /locus_tag="OO328_002807"
     CDS             complement(19139..19876)
                     /gene="ycaL"
                     /locus_tag="OO328_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5931309.1"
                     /translation="MATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIA
                     NQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANG
                     CVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVAS
                     QLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGG
                     RTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4761 a         5512 c         4839 g         4766 t
ORIGIN      
        1 gcgtttcacg cacgttcacg ttgagacgca cgctgttcac ctgaccaaac gatacgttct
       61 gcaacccctg gttgagcata cggatacggt tctgctcgcg ctgaatggtt ttgcgaatga
      121 tgttcgccac gctgcgggaa ctgatcgcca gtttctgttc acgggaggtt aattcttcgg
      181 tcagacggct aagttcaatc tccatctgtt cgatagcttc caccggatca tcggtacgaa
      241 taatatcctg acgaatacgt tcacgcagat gctgataaac cgccacgaag aactgaattt
      301 tacgctccgg acgtttcgga tcttccgaca tgcgcagcac gtcgcgcaga tgttcgttat
      361 ccgccaccgc cagacgcagc gcacctaacg ccttatccga catggaacgc aaatcatcag
      421 cggagagata agccagctca cgacggtgta agcggcgctc aacgccgtta tctttcacca
      481 tgcgcatcac cgcacaccag cccgctttgg cggtcactac ctgctcgcgc atctcaaagt
      541 aatcccgctc cagcttgcgc agtttgcggg tcaggttgtc catctccgct tcgcagaagg
      601 taagcgcttt ttccagttga ttgcggcgtg aacggttatt gctcagttgc gcatgcagct
      661 cgtcacggcg aatacgcgcc cgctcttctg ccccgctatc agcacgcacg ccgatatcct
      721 gcaattcacg ttgcagatcg ttgagtagct cttttttggt gtcgtaagaa cttttcagcg
      781 aagccagcac ctggttgtac tgactcaact gcgctgcgtg accgcgcaac gcttcgcgag
      841 cgcgggtacg ctccgcttcc gcctgttcca gacgttcacg cagtttttcg ttgagatcgc
      901 tgttaccgct aagcatttct gccgagtcag aatagctaaa gtgcgcacga cgctgcacca
      961 cttccgtcag ggcaaacgct tgctgacggg catcgcgctg catctgctga gagtacgcgt
     1021 aatcttcttt taactgttcg aactgttccg ggtcgctctg caataccgaa acgatcggtt
     1081 ccagtttcgc cagttgattg ccaaactgct gaacaaaacg cgcggcttcc tgggcttcat
     1141 ccagacgttc gcggatttca tcgacgcgat ccgccaggct gtcatcagcc aacaggttga
     1201 gacgcggcag aatgcggttc agcgccgtaa cgccttcttt cgcctgctca aactgaatac
     1261 gctgctgctg gttatcattt tcatgattac tcaacgcccg ctccagttcg actcgacggc
     1321 tgttcagttg acggatttct gcttccgggt cagactcaaa cgccaccgcc agatgactgc
     1381 cgataaagcg gctgaacgcc tgatgcagac gctgggtttt ctgtacatca aaggagagcg
     1441 tggcgaagcg ttcggaaagc acttcacgct cggcatggag gctttcaata cggctttcac
     1501 gcgcagcacg accaaacagc ggcacttccg ggaaacgtga ataacgccac tgtcgatcgg
     1561 cgattttcac caccaccgct ttttccagct catcaacgct gaacacgcta tcatcgaatg
     1621 actgcggatc cccttcgatc aaatagagat cttccgggca atcggtcaag ccttccaggt
     1681 gttcggttac ctgtgacaga tctggcacca cgatggcgtg gcgtgacggg ccatacagag
     1741 ccgagaagta cggcgcatct tccaggctaa cgtcgtcata aatttctgac agcagcacac
     1801 caccaaaacg ctccgccagc gcgttcagac gctgatcttc agagccgcca ggctggctta
     1861 aacgttcgat ctcttcatcg acggcgtttt tgcgcgcgcc tacttcatcg cgttcaacaa
     1921 tcgcctcgcg ctcacgctcc agcaactgtt gcagatattc agtgacgtcc tggctggagg
     1981 agaactcttc gccgcactgt tcactcaact ggttgagact gttttgcgct gccagccaaa
     2041 ccggtgcacg ctgcatcaaa ctctgaatgc gagactgcag ctgttcctgt tcctgccgca
     2101 gtgccatccg ctcttcacgg gcgttagata cgctatcgga aagagaggca atgcgtgctt
     2161 ccagctcctg atgcagagct tccagttcat caatatcaaa attcttgccc tgacgtttgc
     2221 agaaatctgc cagcagacgc tcagcttctt gctgctcacg cagacgctgt tccagttcgc
     2281 ttaagcgcat ccgcaacggc tgaacctgct ctgccaggtg acgctgatca accccttcgc
     2341 gcaatagttc gcgagcgaca tcccacgcct cgttacgcgc cagcgggccg ttgattgcca
     2401 ccaccagctg ataagcctgc tcaaactggc tgtgcgcggt ttgcgccatg ctcattttct
     2461 gctcaagaga gagcattttt tcagtcgctt ccagctcttt cgcctggaag gtttccagcc
     2521 attcggcggc gctgtcggcg gttaagtccg gcagatggca cagttctttg gcacgattaa
     2581 gcgcagcaat cgcctggtta tactggatcg cgcgcgtttg ctggacgtcc agcgcctgct
     2641 ggtagtcagc aagctggctt ttcagctcgt ccacttccag ttcggcagct tccgcgcgag
     2701 cctcattctc ttcctggcgt tcgatggctt ctgccaccac ttcattttgc tcttccagac
     2761 ggatctgcag ctcatcgaga tccacttcgt agcgttcgat cttctcctgc tgacgcaatg
     2821 cggtttgcac caggttcagg tgatcgctgg ccgcctgata atccgcttcc agatcacctt
     2881 cggcaccgtt gtgctctgcc agctcacgcg ccatatcgac gtgtttgtac tgctcagccg
     2941 ccagttgctg acgcgaagta tgtagctcgc gacgaaactc cagggctttg tcgagatgga
     3001 cacggcgctc gttggcgtga cgcatgtagt ccgccgccac gtagttggta gcttcgctga
     3061 tcagatgctt aaacaggtca cggtccgact gggtgacacg aatcgcttcc agcgtcatgc
     3121 ggttttcacg cagcgccgct tccatgtcct ggaacgcttt acgcacgccg ctgttttccg
     3181 gcaacaggta gtcgcgcaga gaacgggtaa tggcactgga gatcccgcca tacagcgaag
     3241 cttcgatcag acgatagaat ttgctacggt cagatgcgga gcgcagacga cgcgcgatga
     3301 tgcccagatc gaacatcagc gagtggtaat cggtaatgga gttgaactgt ttaaactgca
     3361 cgccttccat cgcctcgagc ttgtctttca gctcgttaag cggcagcacg cgcgcctggc
     3421 gttcgttcag ggtttcggtc accagctgtg tcggctgcac cgacatcggc agtccctgaa
     3481 tggcaaatgg tttgatatcc actttacgat cgcgtccggc aacctgttgc agacgcacac
     3541 cgaccaccac gcgctggtgg cgcgagttaa tggtgtcgag catcgaataa cagacacccg
     3601 ctttcagctt accgtgcaga cctttatcgc gcgaaccgct ggtggccccg gcttccgtag
     3661 tgttacggaa atgcagcagg gtcaggtcgg ggatcagcgc cgtaacgaac gccgccatcg
     3721 tggtggattt acccgccccg ttaccgccgg aaagcgtcgt gaccagctcg tcaaggtcaa
     3781 aagttcgggc aaaaaagccg ttccagttaa tcagcgtcag tgagcgaaat ttaccgcgtt
     3841 caatcattat tcttcctctc cgctatctgg ctgactctct tcggtttcat cgttgagttg
     3901 cagatgattt tcaatcggca ttgcttcgcc atcacgaatc aggcgacgct gtgcttcacg
     3961 gggatcgtcg ccagcacgca catcggctcc gaagcggaac accgattcgg taatgcggaa
     4021 cttgctgctg tcgtggccca taaaccacac catgcctaaa cgacgcagac ggttgagcga
     4081 agaacgtact ttctcctgca acttctgacg gtcaacgtct gaaccggttg aacggttgtt
     4141 caccagtttc agcagtttcg cttcatcggc cagggtgagc agttcgtcgt acagttcctg
     4201 ctgggtgaaa atcccctcat tcgccagccg ttccgggctg agatagagat aacagaggat
     4261 tttcccgacc atcatatcca gttccgacaa gacggaacgc gggataagag tggtggaacg
     4321 tgggcgtaaa tagaagaacc cttccggtgc gcgaataagc tcaacgttat aacgcgcgta
     4381 aaactcttcc agatattcct gaaaatccat caggaatgca tgattatcca gttcgtcgag
     4441 gccaatatgg cggcctgaac gtaaggcgct gtccagcgcc ggaaataacg gattcgccag
     4501 cgcctgcgcc agcttaaccg gcatcacttg ttcaatattt gtcgatgaca tgcgcctgta
     4561 ccttggctcc gtaatcatta atcggctgcc atttcgctgg cagtccggtg aaatctgctt
     4621 gcgctacgcc aagacgtacc gcctgatcaa taacaatacg cgcaacgtca aagtgacgtg
     4681 cacgcggata ctgtgacaga tattcgcgta ccaccagacc aagatccagc ggcacttgtc
     4741 tggttttgta cacggcaagt tgttcttcga tgatcgccgc cagctgttcg cggatctcgt
     4801 taaactcttc gtattccaga tcctcaggaa gttccccagt cacttcttca tcgcgcagtg
     4861 ccatctcttc gtcacgcata tccagcagac gatcggcatt ggcataagtt agcgcccacg
     4921 gctcatcaaa ataggtttgt accgactgac gtaaccgctg agcaaagacg cggtttttat
     4981 ccatatcgat cgcggtacga ataaatttgt gtacgtggcg gtcgtagcca atccacaagt
     5041 cgatggattg ctggccccaa ctgataatac gatcgagttt gctctgcaga tcgaacacca
     5101 gacgatcgac gaaatgcaga tcgtcatggg tcatcgtcgc atcctgaatg cgcaacagat
     5161 tagcctgcaa tttgtcgcct gccgcttcca gcgtatcctg caattcacgc agcgttccgg
     5221 aagtttccga aagcaacaat tcacagctgg aaatcgccgc ccgccagtct ttgttcagca
     5281 actgggcgat atcgtccttc acctgctgct gctgttcgtc catcagacgt tgcgtcaggt
     5341 cgatactgtc gaaaatttct gctaccgaat atttcagtgg cgcatagaca ttacggtgcc
     5401 agtgaaattc atcaccgccc tcttcggcgg catctgctgc gcgtttgagc tcacccgcca
     5461 caatcgacaa ctgcatagaa agacgcagcg tagaaaactc gcgctgacgg atgtagtagt
     5521 cagtaatgcc gatgccgagc ggcgtcagac ggtaaattgc gttcccttcc gcctgctcgc
     5581 tggtaaagcg gttcagcaga cgttgacgca ccatgtcgtt gatcgcgtta ttggcgcgca
     5641 cgccgatggt ttcgctggtt tgctcaaacg catcactcac atggcggaat gcatccacca
     5701 gctcgccttc actcatctca ccatccagac gctcgccgtt cagcgtggca accgccagca
     5761 gaaaagagag tcggtctacc ggcagcgaga tggagaagtc attttttctg gcccaggcaa
     5821 ccagttcggg gactgtctgg gaaaattcac tcatacttta tccttgctct gcggtttgcg
     5881 cgcggtgaca tgaatataac gccctagggt aatatacggt tcctgacggc aataacgcgt
     5941 ttctaattca agtaatgctt catagcagtc gcgctgctgg tacttctcgc gcagataatc
     6001 atgaaacacg cgaacgcctg tcttacccat aatttgccaa ccagcttctt ccagccacag
     6061 ataaacctgc gccgggtcgc gtggataatc tggcgaaagc gtccgttttt tctttttcgg
     6121 cattcccgcc tgcacgtaat caaaattccc ggcgaccatg ttatgcatca acaaaccatg
     6181 cgcattgtag aacattaacg acaacactcc gcctggacgc aacactgacc acagggtctg
     6241 caatacactg cggggatcgg ccacccactc gagcaccgca tggaacaata tcagatcaac
     6301 gggcgtttcc aaatgcgaag caacatcctg agcggcgcaa tgtataaatt gcatgttgtc
     6361 gctcacacct tttgcttctg ccgcctgttt tgcgcggtcg atcatctgcg cagaaagatc
     6421 gcataaaata acctgatgcc cgcgctcggc cattttgatt gcggtctgcc cttctccgcc
     6481 gccagcatcc agcacacgca gtttttgcgg gcccatttcc gccagcacgc gatcgagatc
     6541 ctgccacaga atagcctgtc gaagctgccc tttggtggtg ccgtaaatgt tacgggaaaa
     6601 cttttccgca atatcatcaa aattgcgatc ctgcattgct ttctccaccg gttggctaag
     6661 acgttttctg ataaagacgc tattttgtca cagccacggc gagaatgaac ccgtctggtg
     6721 taatatcgct agcgatccct tgctatatgg ttaaaaaagg aaccagaaag gatgcttttt
     6781 acactgaaaa aagtgattgg caatatgttg ctgccacttc cgctgatgtt gttaattatt
     6841 ggcgctggcc tggcgctgct gtggtttagc cgttttcaaa aaaccgggaa gatttttatt
     6901 agcattgggt ggctggcact cttgttattg agtttgcaac cggtagccga ccgcctgctg
     6961 cgtcccatcg aaagcaccta tccgacatgg aataattcgc aaaaagtgga ctatatcgtg
     7021 gtgctcggag gtggttatac ctggaacccg cagtgggcac cgagctctaa tttaatcaat
     7081 aacagtctgc cacgcctgaa tgagggtatc cgtttatggc gcgaaaatcc gggttcaaaa
     7141 ctgatcttca cgggaggcgt agcaaaaacc aatacggtga gtacagcgga agtaggtgcc
     7201 agagttgcgc aatcgctggg cgtaccgcgc gagcaaatta tcaccctgga tttgccaaaa
     7261 gataccgaag aagaagctgc ggcagtgaaa caggcgattg gtgacgcccc tttcctgctg
     7321 gtgacctccg catcacatct gccgcgggcg atgatctttt ttcagcagga agggttaaat
     7381 ccgctaccag ccccggcgaa tcagttggcc atcgactcgc cgcttaatcc gtgggaacgg
     7441 gcaatccctt ctccggtctg gctgatgcat agcgatcgtg ttggctatga aacgcttggc
     7501 cgcatctggc agtggctgaa aggtccgtca ggcgagccac ggcaagagtg attttgatgc
     7561 cagatcgaag ttgcgccgac taaatcgtcc ggtattaacc agttgcgcaa cttcatccca
     7621 taacacatac aaccaacgcc gccagatgaa cgactccgcc actggcgcgc gttgcaggta
     7681 actccagagc aaatcttccg ccagagaatt atccattagt cggaacagtt cgtattctcg
     7741 cggtgcccaa agcattagtc ccggcccgac catcgccagt aactgatcgc tgcgcgaatc
     7801 tttcagcatg ctgcgtaaac agaagttacc gtgaatcaac acacaattgt cgttaaagcc
     7861 ttcaaataat gccgggagac attcgcgagt gcgaaacagg atccgcttat cctgcatcgt
     7921 cagaccggtg ttattgaact gattgagcgt ggtccatagc acttcaacat gttgccggta
     7981 ccatgagggc cagaaatttt cctgagtatt gtcgaccgcg ccgacgcaac cgcgactgtc
     8041 ctgacggtgc caggccagta aggcttcaac gatttggtct ttgagctgtt cccagcgttc
     8101 tggtgttcgg gctggtgcct ccaccgaaac accacgcatc cgctccagta gcaggacatc
     8161 agggccgggg tgttcttcat gcgtcatcac accataaata gtcggcatac ggacagtccc
     8221 acttcttgcc agcatggtgg ttttccatgc cagttgtcgg gcttttccgg gcgtactaaa
     8281 gcttcttgcc attaacggca ttgggtttcc ctggctgtca tacaaagccc acaacgccgt
     8341 atccgctttt tcattgacgc actcaatacg actgagtttt tcgcccagta aatggcttaa
     8401 ttcggcacgc agctgttcca ttcagattac cctcataaaa gctactgctt tcataatgag
     8461 cgccaatata aaagatgtca cctgaatgag atcaattgtt gaaaagaaaa attgaggaaa
     8521 atcgtaacgg tacgacactc ctcccaaaat tggctgaagt gtcgtgaagt gaaattagcg
     8581 catttcagcg cgaacgcgct caagatcttc aggggtatcc acacctgtgc caggaacttc
     8641 ctgagcaaca gcaacatgga ttttttcgcc gtaccacaga acacgaagct gctctaacat
     8701 ttcgatgtgt tctaacggac ttgcctgcca gttgacgtaa cgacggataa agcctgcacg
     8761 gtagccataa ataccaagat gacgcaggaa gttatcgcca acggtttcaa ggccttctgc
     8821 aaaacgatca cgatcccaag gaatggtggc acgtgagaag tacagtgcat acccttcagc
     8881 gtcgagaacc actttcaccg cattcgggtt aaacgcttct tccgcattgt ggattggcac
     8941 cgccagagtc gccataccca cctgacgctg agcgaggtta tcagcaacct gacgaatgat
     9001 tgtcgcaggg atcatcggtt catcaccctg cacattaacg atcaccgtgt cgtcgctgaa
     9061 tgcgcatttt tcgacaactt ccgccagacg ttctgttcct gactgatgat cggcgcgcgt
     9121 catacatact tcaccgccag cggcttcaac ggcgcgggca acatcatcat gatcggttgc
     9181 cacgatgatg cgctcggcac ctgattcacg cgcgcgttca agaacatgaa caatcatggg
     9241 tttgccgtta atatcaacca atggtttacc gggcagacgc gtggacgcgt agcgcgcggg
     9301 aataatgacc acaaaactca tgatttactc tcatcagcag tcagcacgcg ggcttccgtt
     9361 tccagcaaca ctggaatgcc atcgcggagc gggaaagcca ggttatccag tttgcaaata
     9421 agctcttgtt tttcctggtt ataccaaagt tttccgttgc aaaccgggca ggcaatgatt
     9481 tcaagcaaac gatgatccat agttcctcca gatggatcgg gttatgaatg cataaatctt
     9541 atcataatca tttatgcgac ggggtctatt tcccagccag tgcggcaatc agtaaagaga
     9601 ccttgcgggc agcgtcctaa tgtcacccgc gttgcctgct gccagctcgc gaaatcagta
     9661 atcgcctgac gtaacccttt ttgcagcatt gtcgttggtt taatgccttc ctgtaaccac
     9721 agagagataa cttcaaggat gcctgtctgg cgatgcattt tggcatccat tcgcccaact
     9781 aattgcccac gatgtaataa tggcagaaca aaatagccat actggcgttt cggcgctggg
     9841 gtatagcact ccagccggta gctaaaatca aaaagctgct ctgcgcgttt gcgatcccag
     9901 acaacaggat caaaaggcga aagtaccgcg ctgtgcgttg cagtgagctt tccggccagc
     9961 gcacgctcga gtagcggcag caaatcagca tgcagccaaa gattgcccaa tttttcaaca
    10021 tgcacagcaa tgatttgctg ctgttcagcc ctcgcttcgc gccacgccgc cagtgccgga
    10081 cgtttcagcc gatagtaatc tgccagccac tgttcgcgga atattcccag gctacgcgca
    10141 ctgttatcca gcatgatgat ttctgcttct gtttgcgaaa cgagatcgcg ctcatcatcc
    10201 cagtcaggca tgacacggtg ggttaaatca taaacgcgct ggaagttgcg ccgttcaatc
    10261 accatcacct ttccggcagt aaataaacct tccagatgcc gtttatgcgg cttccattcc
    10321 caccagccgc ttgcaccttt acgaggatgc tcaaaatcgg ctgaacgtac cggcccttta
    10381 tcatgaatat gctgaattaa ctgtgcaatt tccgcctcat gttcctgcat ccaggcgtct
    10441 ttgtatttcc agcccatttt ttcaggtgcc agcatgcggt ggcgaataag acgaaagtcg
    10501 ctacgcggca taaagcaggc ttcatgcgcc cagtattcca ttaattcgcc acgcgccaga
    10561 gactcatcca gccactgggc aggataattt cccagacgac tgaaaagcac cagatatgga
    10621 ctacgggcaa caatattgat ggtatcgatt tgcagcaagg acatgcggga gatcgttgcc
    10681 ggaatatcct ccaacgacgc tcgacggcgg ggtttgttta acaggccttg tgcggcaagg
    10741 tgaagattac gcgcatcagc aagggagagg tgcggcagcg acattcatga ctccatcaat
    10801 cgaacgctgc cgcggcgtaa ctagttgcca gaagccagcg aggttagttg cgtaagcagt
    10861 ttcgctggtt catcacctga aagctgtgcg tctacaggca aataccacca attttcttct
    10921 gcaaaggccc ggcatttcac cgcatctttt tcagtcatta ccagcgtttg cccggcgctt
    10981 accaacgcac tgacatcctc atggttcaaa gactgatgat cggccagcgg tacacatttt
    11041 tccggttgta cgccacacat cttcagcgtg gcaaaaaagc gcggcggatg cccaatcccc
    11101 gccatcgcca ctacatgttc aagctgagca acgtcacaac gcgtaccggt acgtaaattc
    11161 accgcctgac ccggcagcag atgcatgggg atttcaccgc tgcgagggac accgccgttg
    11221 acgattaccg catcaaccga ctttaagcgc cccgctcgct cacgcattgg ccccgccggc
    11281 aaccaccagc cattgccaaa gcgacgcaca ccatcaataa cgacaatttc cacatcacgc
    11341 gccagacggt aatgctgtaa accgtcgtcg gttacgatga tctgcacatc agggtgttgc
    11401 gccagaatgg cttttaccgc atcagaacga acgggagaaa ccgcaacagg cgcatcagtg
    11461 cgttgataaa tcaacacagg ttcatcaccc gcctgtgctg tggtggtatc tgccgacaat
    11521 aacagcggat aagattcagc cttaccacca tatccccgcg atacgacccc cacgcgaata
    11581 ccgcgctgtt gcaactgttc caccagccag acaacgaccg gggtttttcc gttgccgcct
    11641 gcggtgagat taccaaccac gacaaccggt acgggggcac gccaggcgcg cttcagtttt
    11701 agtttatagc aaagacggat cgcgccactc accaggccat acaaccagga gagtggcagc
    11761 aatagccgcc acaaagggga ttcaccagac cagatttttt cgatcattgg ccaaactgca
    11821 ttttgtgaag ttgcgcgtaa acgccgcggt gctcaagcaa atcgttatgc gtaccgcgtt
    11881 ccacaatgac accatcctcg acgaccacga tttcgtctgc cttttcaatg gtagacaagc
    11941 ggtgggcaat caccagagag gtacggtttt tctgcaactc atccagtgcc gcctgaatcg
    12001 cacgttcgga ttcggtatcc agagccgagg tagcttcatc cagaatcaga atcgggctat
    12061 cacgcagcaa ggctcgagcg atagcaatac gctgacgctg accgccagag agcagcacgc
    12121 cgttttcacc aatcactgta tcgagaccgt tatccatctt attgatgaag tccatggcgt
    12181 aggccatccg cgccgcttct tcaatttgct cacggctgta ctgttcagtc cgtgcgtaag
    12241 caatgttgtt agcaaccgta tcgttaaaca gatggacatt ctgcgacacc agagcaacct
    12301 ggttacgtaa cgacgccagg gtatactcgc gcagatcgtg accatccatc aggatttcgc
    12361 cttcatcaat atcgtaaaaa cgcgtgatca ggctggcgat ggttgattta cctgaaccag
    12421 agcgtccaac cagagcaacc gtcttccctg ccggaatttt caggttgatg ttacgcaatg
    12481 cgggtacgtc acgtcccgga taagtaaagg tgacattgcg gaattccacg tcgccagtcg
    12541 cacgctcgat cacgcgctta ccttcgtctt tctcctgctc actgtccaga atggtaaaca
    12601 gcgtctgaca agccgccata ccgcgctgga actgggcgtt aacgttggtc agcgatttca
    12661 gcggacgcat cagtgcaatc attgaagaga aaacaacggt aatcgtaccg gcagtcaggt
    12721 tatccataac acttgggaag ctcgccgcat acagaacaaa cgccagcgcc aaagaggcga
    12781 tcagctgaat gatcggatca gagatggaag aggctgaaac cattttcatc ccctgaagac
    12841 gcattcggtt actgacttta tcgaagcgtt tcgtttccac ttcctgacca ccgaaaatca
    12901 atacttcttt gtggcccttc agcatttgtt ctgcactggt ggtcacttgc cccatggtgt
    12961 tctgcatgtt tttactgatg ttgcgaaaac gcttcgatac tacgcgaatc gcaatcgaaa
    13021 caatcggtgc cagcacaatc aaaatgatcg acagttgcca actgtaatag aacatcatga
    13081 tgaacaggcc gatgatcgac gcaccttcac gcacaacagt aatcagtgcg ccggaagaag
    13141 aagaagcaac ctgttcggaa tcgtaggtga tacgtgacaa cagcgtaccc gttgactgtt
    13201 tgtcaaagaa tgaaactggc attcccatca tgtgaccaaa caggcggcga cgcatggtca
    13261 ttaccacctt tcctgatacc caggagatac agtagctgga gacatagctg gtgataccac
    13321 gtaaaatcat cagtccgatc accaccagcg gcatccacac cagcacggag cgatctgttt
    13381 taccaaagcc atcatcaaga agtggcttaa ggagcgataa catgaaggta tcgctggctg
    13441 cgttgaggat taacgctacg cccgccacga tcagacccgc tttgaaaggc gcaatggttg
    13501 gccacagtcg gcggaatgtc tgccacgtag agagatcttt gtcgttatgc attcaaaaaa
    13561 ccagcatttg ttgaaatagc cgcatattct acccgttatc cactggcacg ccaaaccact
    13621 gatgatacca acgaggtaaa atttgctctc tgaggctgct aatccgccag ccttgtgctg
    13681 aaaaattgac cgtcacttgc ccctgatgtg gagtatcaag ccattgatat cctttctgtt
    13741 gatagcgatg cttaactttg ttagagggca atcgccatgc gttatagcgc gatgccgatg
    13801 cgagtgccac ttttccattc actcgctgaa ttaatggcaa cgatgatgag gtattactgc
    13861 catggtgagg tacctgaagc aatgttgcct gcacttgctg ccagtaacgg cttagcatct
    13921 tttgttcagc tggggcttca atatcaccgg ttagaagaat gctattcgtc ccgtcatcaa
    13981 ccttaaccac acaggaatgg ttatttccct tatcgttgct accttgtaaa ggccagtgcg
    14041 cgctgaaacg caatccttgc cactgccacg cttcgccacg cacacagggc tgatgatgtt
    14101 cccagtttaa cggacttctg atccataaca tcggccatat atgcaatatt gaatccagcc
    14161 ctccccggtg atccagatgt tcatggctca gaataacgcc ttccggttca agattatgcc
    14221 agtggagcca ggggataatc agttgttgcc cactatcccc ttcaggccag tcctgtgtca
    14281 tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc gacatcaagc
    14341 atgtacagct gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagcaag
    14401 cctccagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt
    14461 aagaaccatg gggaaaatga tagccattgc caacattcaa caatgttgat ccacccttcc
    14521 ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac
    14581 ccctgttcca ggattaacgg cccgctaaaa tgcacaacca tcgcggcgag gatcaacgga
    14641 accgtgataa atgtcaccaa gggaattgca aatagatttg caataaacga ggtcagacta
    14701 atgccatgaa atatgacgat ttgcatgggc ataagcagga gtgtgattcc cagttgcaga
    14761 tggatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac
    14821 cactgatacc aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag
    14881 agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag
    14941 ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta
    15001 cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagattccg
    15061 ccaattaatg gtatttgcca gtggatccag cgcccaggca gaaaaatttg tccactgcga
    15121 atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa
    15181 tgcgccgttc cagtatcgcg catgatattt ttgatttctt tggggacgga taaccgttcc
    15241 cccataccta aaccaagaat aaccgcattc cacggatagg gttgcagcgt tgtttgtagt
    15301 gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc ctgcagaaaa
    15361 cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca
    15421 ttaagttggc catgaactgc acgaactttg agtttcattg accattgttg tccagcacaa
    15481 accgcttgcg gaagttattc gccatacagc acaaggcctg gcgcagggaa tatacgtcga
    15541 ccttgtagat aagtaatttg accgtaatga gtggtcatgc cgtcagttgc agtgatctca
    15601 acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat ttgctttgct
    15661 gataatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac ggttttaaca
    15721 gggataaatg ccagtacgca ggcgaagaga gtcagaaacg caagggttaa tgtcctaggc
    15781 aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt
    15841 attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg
    15901 tgggaatttt gcgttccccg aggcacattc agaagaattt aacgaaaggg tgaaaactgc
    15961 tcaaaactct gcgaggcagc ttccagttcc tgattagaga attgaaaaaa gcacccgaca
    16021 ggtgcttttc tcgcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc
    16081 gcagttcttt accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc
    16141 cagtcttcgg attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac
    16201 cgcggatttc aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt
    16261 ttactgcatc ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta
    16321 tcaattctga cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt
    16381 tgctgcattg attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct
    16441 gcaatctgtc aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt
    16501 actcgccttt agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt
    16561 cctgtttgtt aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag
    16621 acaggctgat tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc
    16681 caacgctcag aaccagggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca
    16741 ggtaaccttc aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta
    16801 ctttaccggt tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt
    16861 ctgcgagctg tttaacgccc agggagatac gttcacgttc tgcgtcaacc tgcagaacaa
    16921 ctgcagcgat ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc
    16981 aggagatgtc agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac
    17041 cgaagtcagt gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg
    17101 cgaactgctg ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt
    17161 cttcgtcgat atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg
    17221 ggtggatgtt tttgttggtc caatccattt cggaaacgtg taccagacct tcaacgcctt
    17281 cttcgatttc aacgaagcag ccgtagtcag tcaggttggt cacgcgacca gtcagtttgg
    17341 taccttccgg ataacgttta gcgatagcta cccacggatc ttcgcccagc tgtttcaggc
    17401 ccagggatac acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc
    17461 ccacgttgac gatttcgctc ggatgcttaa cgcgtttcca ggccatgtca gtgatgtgca
    17521 gcaggccgtc aacgccgccc agatcaacga atgcaccgta gtcagtgagg ttcttaacga
    17581 tacctttaac ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt
    17641 tttcggattc gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct
    17701 tgattacttt aaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa
    17761 cgtctaccag agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc
    17821 ccttaacttt gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca
    17881 gcgtgatcca ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc
    17941 cgtcttctac tgcgtccaga gcaacgtcaa cttcgtcacc tacctggatt tccagctcgc
    18001 cctgggcgtt tttgaactgc tcagccggga tggcggactc agatttcaga ccagcgtcaa
    18061 ccagtactac gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg
    18121 tttcgatttc ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc
    18181 ttcaggttta tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc
    18241 gctgtcattc cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt
    18301 ctggcgcgcg tattgtagcg ctttttcaat caattgctca atgcttaagg tggtggaatc
    18361 caacactaaa gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc
    18421 gtcgcgttct ttgatctcgg ccaaaaggcg ctcgaagtta acactaaagc ccttctcctg
    18481 caactgtagc atgcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac
    18541 tggtgcatca gggaatacca ccgttcccat gtcgcggcca tcggcaatca gacctggtaa
    18601 ttcgcgaaac gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac
    18661 ttgtgaagcc gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc
    18721 gaggatcact tccagattgc cattggtcga cacaaaacgt acatccagat gggatgccag
    18781 cggtaccagc gcatcttccg acgcaacatc aacatggtga tgtaatgccg ccaatgccag
    18841 tacgcgataa attgcacccg agtccagcag atgccattgc aacgcttccg ccatagcctt
    18901 acacaaggtg cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc
    18961 cgtcatcttt atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg
    19021 ccgacgccac aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg
    19081 gatttattgc gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt
    19141 acttaccaga ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag
    19201 agtcaaacat ggactgggtg cgaccgccat ccaggctagc cagtttttca aagctgccaa
    19261 ccagcccctg ggtgctgatg ccacgtttct tcaacagatc aaaagagaaa tcatctgctt
    19321 cggactcttt atcacgggag tatttagcat taatagcgcc ttctgcgata tcacccaatt
    19381 gtgagcggga aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa
    19441 cgatcgcata agaagctttc atttcagcca gcgagtgacc caacgcgacg tggcccagtt
    19501 catggcccag aacaccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga
    19561 cacgaacaca gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat
    19621 agttgaccgg cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt
    19681 taccgtactt gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct
    19741 gattggcaat cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac
    19801 cggagctgat agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg
    19861 ctgcagaggt cgcaatcg
//
LOCUS       DAJTRN010000077        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRN010000077 DAJTRN010000000
VERSION     DAJTRN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO328_002808"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO328_002808"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5931310.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO328_002809"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO328_002809"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5931311.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO328_002810"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO328_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5931312.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO328_002811"
     CDS             6038..6658
                     /locus_tag="OO328_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5931313.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO328_002812"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO328_002812"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5931314.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO328_002813"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO328_002813"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5931315.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO328_002814"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO328_002814"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5931316.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO328_002815"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO328_002815"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5931317.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO328_002816"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO328_002816"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO328_002817"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO328_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO328_002818"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO328_002818"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5931318.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO328_002819"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO328_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5931319.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO328_002820"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO328_002820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5931320.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO328_002821"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO328_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5931321.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO328_002822"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO328_002822"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5931322.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO328_002823"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO328_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5931323.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTRN010000078        18888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRN010000078 DAJTRN010000000
VERSION     DAJTRN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..2092
                     /gene="pbpC"
                     /locus_tag="OO328_002824"
     CDS             <1..2092
                     /gene="pbpC"
                     /locus_tag="OO328_002824"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5931324.1"
                     /translation="RYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGST
                     LTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGAA
                     SWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSR
                     EQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWK
                     GRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYG
                     LALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAY
                     GPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRL
                     QPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAI
                     GVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTR
                     GVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQR
                     VAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVR
                     DGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVM
                     DDVGQIATVKFVMQ"
     gene            2242..2673
                     /gene="ndk"
                     /locus_tag="OO328_002825"
     CDS             2242..2673
                     /gene="ndk"
                     /locus_tag="OO328_002825"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5931325.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2823..3977
                     /gene="trmG/rlmN"
                     /locus_tag="OO328_002826"
     CDS             2823..3977
                     /gene="trmG/rlmN"
                     /locus_tag="OO328_002826"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5931326.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4262..5275
                     /gene="rodZ"
                     /locus_tag="OO328_002827"
     CDS             4262..5275
                     /gene="rodZ"
                     /locus_tag="OO328_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5931327.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5302..6429
                     /gene="ispG"
                     /locus_tag="OO328_002828"
                     /gene_synonym="gcpE"
     CDS             5302..6429
                     /gene="ispG"
                     /locus_tag="OO328_002828"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5931328.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6532..7806
                     /gene="hisS"
                     /locus_tag="OO328_002829"
     CDS             6532..7806
                     /gene="hisS"
                     /locus_tag="OO328_002829"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5931329.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7824..8444
                     /gene="yfgM"
                     /locus_tag="OO328_002830"
     CDS             7824..8444
                     /gene="yfgM"
                     /locus_tag="OO328_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5931330.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8455..9633
                     /gene="bamB"
                     /locus_tag="OO328_002831"
     CDS             8455..9633
                     /gene="bamB"
                     /locus_tag="OO328_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5931331.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9751..11223
                     /gene="der"
                     /locus_tag="OO328_002832"
     CDS             9751..11223
                     /gene="der"
                     /locus_tag="OO328_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5931332.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11293..11508
                     /locus_tag="OO328_002833"
     CDS             11293..11508
                     /locus_tag="OO328_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5931333.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11505..12875)
                     /gene="xseA"
                     /locus_tag="OO328_002834"
     CDS             complement(11505..12875)
                     /gene="xseA"
                     /locus_tag="OO328_002834"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5931334.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13037..14503
                     /gene="guaB"
                     /locus_tag="OO328_002835"
     CDS             13037..14503
                     /gene="guaB"
                     /locus_tag="OO328_002835"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5931335.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14572..16149
                     /gene="guaA"
                     /locus_tag="OO328_002836"
     CDS             14572..16149
                     /gene="guaA"
                     /locus_tag="OO328_002836"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5931336.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16244..16783)
                     /locus_tag="OO328_002837"
     CDS             complement(16244..16783)
                     /locus_tag="OO328_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5931337.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16799..17317)
                     /locus_tag="OO328_002838"
     CDS             complement(16799..17317)
                     /locus_tag="OO328_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5931338.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17628..17819)
                     /locus_tag="OO328_002839"
     CDS             complement(17628..17819)
                     /locus_tag="OO328_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5931339.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17837..17989
                     /locus_tag="OO328_002840"
     CDS             17837..17989
                     /locus_tag="OO328_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931340.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18171..>18888
                     /gene="pdeF"
                     /locus_tag="OO328_002841"
     CDS             18171..>18888
                     /gene="pdeF"
                     /locus_tag="OO328_002841"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5931341.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKST"
BASE COUNT         4460 a         4596 c         5150 g         4682 t
ORIGIN      
        1 acgttacctt gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt
       61 taatccattc tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc
      121 cggtggcagc acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt
      181 tggtggcaaa attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg
      241 tgaaattctg atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg
      301 tgcggcaagt tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc
      361 aatgctggcg gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg
      421 tgccgaagcc gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg
      481 tgagcaggta aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca
      541 actggcaccg ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac
      601 tacgctggat gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg
      661 gttgccaccg cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg
      721 cggctgggtg ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt
      781 caatgcgatc cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga
      841 tgaaggcttg atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta
      901 tcgaccaggt aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt
      961 gcgctcgctg aacttacctg ctgtgcaggt gctggaagcc tatggaccga aacggtttgc
     1021 ggcaaagtta cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct
     1081 ttcactcatt ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc
     1141 gtttgctcgc cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga
     1201 acggccttta atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc
     1261 gcaacccttg ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac
     1321 cagctatggc tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat
     1381 ctggactggc agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt
     1441 accattgttg aatcaggtca ataatatctt actgtcgcgc agtgcgaatc tgccggaaga
     1501 cccgcgcccg aactcagtca ctcgtggcgt tatctgctgg ccgggcggac agtctttgcc
     1561 ggaaggtgac ggtaactgtc gccgccgcct ggcaacctgg ctgctggatg gcagccagcc
     1621 gccaacgctg ttactgtcag agcaggaagg gattaatggc attcgcttcc ccatctggct
     1681 ggatgacaaa gggcagcgcg tcgccgctga ctgcccgcag gcgaagcaag aaatggtaaa
     1741 tgtctggccg ctaccgctgg aaccctggct gcccgcatca gaacgtcgcg ctgtgcgctt
     1801 accaccggcc tcgacaattt gcccgccata cggtcacgat gcccagctcc ctctgcagct
     1861 gacaggcgtt cgcgatggcg cgattattaa acgtttaccg ggcgcagcgg aagcgacttt
     1921 gccgttgcaa tcaagtggag gggcaggtga acgctggtgg tttctgaatg gcgaaccgtt
     1981 aactgaacgc gggcgcaacg tgaccctgca tttgacggat aaaggtgatt atcaattgct
     2041 ggtgatggat gatgtggggc aaatcgcgac agtgaaattt gtcatgcaat agtctgtttt
     2101 gtccctgatt gttgctaaaa ctcattttat tttaaaaaaa atgttacctt catcaatagt
     2161 caacggccct gttgctcatt ataatccgcg ccatctcgta cgctggtaca gacaacaaca
     2221 gaacaattta cagaggtaaa aatggctatt gaacgtactt tttccatcat caaaccgaac
     2281 gcggtagcaa aaaacgtcat tggtaatatc tttgcgcgct ttgaagctgc agggttcaaa
     2341 attgtcggca ccaaaatgct gcatctgacc gttgaacagg cacgtggctt ttatgctgaa
     2401 cacgatggaa aaccgttctt tgatggtctg gttgaattca tgacctctgg cccgatcgtg
     2461 gtttccgtgc tggaaggtga aaacgccgtt cagcgtcacc gcgatctgct gggcgcgacc
     2521 aatccggcaa acgcactggc tggtactctg cgcgctgatt acgctgacag cctgaccgaa
     2581 aacggtaccc acggttctga ttccgtcgaa tctgccgctc gcgaaatcgc ttatttcttc
     2641 ggcgaaggcg aagtgtgccc gcgcacccgt taataatttc gtaaatgccg cgtgcaaacg
     2701 tggcatccgt gcgccagaat ttgtacaatg cagcgccccc ggacgagcag ccgctcaccg
     2761 gggcgtttct tttttcaacc ctccaggggc cataacgtgt aataacgagg ccggaatagc
     2821 atatgtctga acaattagtc acacctgaaa acgtcaccac gaaagatgga aaaatcaacc
     2881 tgctggatct caaccgtcag cagatgcggg agttttttaa agatttaggt gaaaaaacct
     2941 tccgcgccga tcaggtgatg aagtggatgt atcactattg ctgcgacaac tttgatgaga
     3001 tgaccgacat caacaaagtg ttgcgcggca aactgaaaga ggtggcggaa atccgcgcac
     3061 cggaagtggt tgaagaacag cgttcatccg acggcaccat taaatgggcg atcgccgttg
     3121 gcgatcagcg cgtcgaaacg gtgtatatcc cggaagacga ccgtgccacg ctctgcgtct
     3181 cttcgcaggt ggggtgtgcg ctggagtgta aattctgttc caccgcccag cagggcttta
     3241 accgcaacct gcgggtatcg gaaattattg gccaggtgtg gcgtgcggcg aaaatcgtcg
     3301 gcgcagcaaa agtcaccggt cagcgcccaa tcactaacgt cgtgatgatg ggcatgggcg
     3361 agccgctgct caacctgaac aacgtcgttc cggcgatgga aatcatgctt gatgatttcg
     3421 gttttggcct gtctaaacgt cgcgtgacgc tttccacttc cggtgtagtt ccggcgctgg
     3481 ataaactggg cgatatgatc gacgttgcac tggcaatttc cctgcacgcg ccgaacgacg
     3541 aaattcgtga cgaaattgtg ccgatcaaca aaaagtacaa tatcgaaacg ttccttgctg
     3601 cggtgcgccg ttatctggag aaatccaacg ccaatcaggg ccgagtcact attgaatacg
     3661 tgatgcttga ccacgttaac gacggcactg aacacgcgca ccaactggcg gaactgctga
     3721 aagatacgcc gtgtaagatc aacctgatcc cgtggaaccc gttcccggac gcgccgtatg
     3781 gacgcagttc gaacagccgt atcgaccgtt tctcaaaagt gctgatgagc tacggtttca
     3841 ccactattgt gcgtaaaacg cgtggtgatg atatcgatgc tgcctgtggt cagttggcgg
     3901 gcgatgttat cgaccgtacg aaacgtactc tgcgtaaacg gatgcagggt gaagccatcg
     3961 atattaaagc ggtctgatag ccattacgcc acggttactt tatgtaccgt ggcgtaatct
     4021 tttatgaatg aagtattaag ttgcaattgt ctggtcaata ttaacggtgc gtttttgctg
     4081 actttaaggc agtatgtaac gatgcaacag taagttagcc tcagtgaatg tgggctttgt
     4141 cacgagcaca cagacggtct tatactgtat gataacggtt aacttaacgg atgtttcgcg
     4201 gtgtgggtga gcatgatggt tcaccggcat ctcaattctc atttaaacgt acctgcagcg
     4261 aatgaatact gaagccacgc acgaccaaaa tgaagcactt actaccggcg ctcgcctgcg
     4321 taatgctcgc gaacaactag gacttagtca gcaggccgtt gccgagcgac tttgcctgaa
     4381 ggtttccacg gtacgcgaca ttgaagaaga taaggcaccc gccgatcttg cttcaacatt
     4441 cctgcgcgga tatatccgct cttatgcgcg tctggtacat attccagaag aagaactgct
     4501 gccagggctg gaaaagcagg ctccacttcg ggctgcaaaa gttgcgccaa tgcagagttt
     4561 ttccctcggt aaacgccgca aaaaacgcga cggctggcta atgaccttca cctggctggt
     4621 gttgtttgtg gttatcggcc tgagcggtgc ctggtggtgg caagaccaca aagctcagca
     4681 ggaagagatc accactatgg ccgatcaatc ttcggcggaa ctgagcagta atagcgagca
     4741 ggggcagagt gttccgttaa atacgtcgac aactacagac ccggctacaa cgtccacgcc
     4801 gccagcgtct gtggatacta ccgcaaccaa cacgcaaaca cctgccgtaa ctgcgccagc
     4861 accagctgtt gatccgcaac agaatgcggt tgtttcgcct tcgcaggcaa atgttgatac
     4921 cgccgcgacc ccggtaccaa cggcagcaac aacgccagat ggtgctgcgc cgttgccaac
     4981 cgatcaggct ggcgtgacca cgccggcggc tgatccgaat gcgctggtga tgaactttac
     5041 tgccgattgc tggctggagg tcactgatgc taccggtaaa aaattgttta gcgggatgca
     5101 gcgtaaagac ggtaatttga atttaaccgg ccaggcaccg tataaactga aaattggtgc
     5161 gccagccgca gtacagatcc agtatcaagg gaaacctgtc gatctgagtc gttttatcag
     5221 aactaaccag gttgcgcgtc tgaccctcaa tgccgaacaa tcaccggcgc agtaacagac
     5281 gggtaacgcg ggagattttt catgcataac caggctccaa ttcaacgtag aaaatcaaca
     5341 cgtatttacg ttgggaatgt gccgattggc gatggtgctc ccatcgccgt acagtccatg
     5401 accaatacgc gtacgacaga tgtcgaagca acggtcaatc aaatcaaggc gctggaacgc
     5461 gttggcgctg atatcgtccg tgtctccgta ccgacgatgg acgcggcaga agcgttcaaa
     5521 ctcatcaaac agcgggttaa cgtgccgctg gtggctgaca tccacttcga ctatcgcatt
     5581 gcgctgaaag tagcggaata cggcgtcgat tgtctgcgta ttaaccctgg taatatcggt
     5641 aatgaagagc gtattcgcat ggtggttgac tgtgcgcgcg ataaaaacat tccgatccgt
     5701 attggcgtta acgccggatc gctggaaaaa gatctgcaag aaaagtatgg cgaaccgacg
     5761 ccgcaggcgt tgctggaatc cgccatgcgt catgttgatc atctcgatcg cctgaacttc
     5821 gatcagttca aagtcagcgt gaaagcgtct gacgtcttcc tcgctgttga gtcttatcgt
     5881 ttgctggcaa aacagatcga tcagccgctg catctgggga tcaccgaagc cggtggtgca
     5941 cgcagcgggg cagtaaaatc cgccattggt ttaggtctgc tgctgtcgga aggcatcggc
     6001 gacacgctgc gtgtatcgct ggcggccgat ccggtcgaag agatcaaagt cggtttcgat
     6061 attttgaaat cgctgcgtat ccgttcgcga gggattaact tcatcgcctg cccgacctgt
     6121 tcgcgtcagg aatttgatgt tatcggtacg gttaacgcgc tggagcaacg cctggaagat
     6181 atcatcactc cgatggacgt ttcgattatc ggctgcgtgg tgaatggccc aggtgaggcg
     6241 ctggtttcta cactcggcgt caccggcggc aacaagaaaa gcggcctcta tgaagatggc
     6301 gtgcgcaaag accgtctgga caacaacgat atgatcgacc agctggaagc acgcattcgt
     6361 gcgaaagcca gtcagctgga cgaagcgcgt cgaattgacg ttcagcaggt tggaaaaata
     6421 ataacgtgat gggaagcgcc tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa
     6481 cattgaggga agcgttgagg gttcattttt atattcagaa agagaataaa cgtggcaaaa
     6541 aacattcaag ccattcgcgg catgaacgat tacctgcctg gcgaaacggc catctggcag
     6601 cgcattgaag gcacactgaa aaacgtgctc ggcagctacg gttacagtga aatccgcttg
     6661 ccgattgtag agcagacccc gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt
     6721 gaaaaagaga tgtacacctt tgaggatcgc aatggcgaca gcctgactct gcgccctgaa
     6781 gggacggcgg gctgtgtacg cgccggcatc gagcatggtc ttctgtacaa tcaggaacag
     6841 cgtctgtggt atatcgggcc gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt
     6901 cagttccatc agttgggctg cgaagttttc ggtctgcaag gtccggatat cgacgctgaa
     6961 ctgattatgc tcactgcccg ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt
     7021 gagctgaatt ctatcggttc gctggaagca cgcgccaatt accgcgatgc gctggtggca
     7081 ttccttgagc agcataaaga aaagctggac gaagactgca aacgccgcat gtacactaac
     7141 ccgctgcgcg tgctggattc aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg
     7201 gcattaggtg attatctgga cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg
     7261 ctggagagcg cggggatcgc ttacaccgta aaccagcgtc tggtgcgtgg tctggattac
     7321 tataaccgta ccgttttcga gtgggtgact aacagtctcg gctcccaggg caccgtgtgt
     7381 gcaggcggtc gttatgacgg tcttgtggaa caactgggcg gtcgtgcaac accggctgtc
     7441 ggttttgcga tgggcctcga acgtcttgta ttgttagtac aggccgttaa tccggaattt
     7501 aaagccgatc ctgttgtcga tatatacctg gtggcttcag gtgctgatac acaatctgcg
     7561 gctatggcat tagctgagcg tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac
     7621 cacggcggcg gcaactttaa gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt
     7681 gctgtggtgc tgggtgagtc tgaagtggct aacggcacag cagtagtgaa ggatttgcgc
     7741 tctggtgagc aaacggcagt tgcgcaggat agcgtagccg cgcatttgcg cacgttactg
     7801 ggttaaggaa ggagaaggac agcgtggaaa tttacgagaa cgaaaacgac caggtagaag
     7861 cggttaaacg cttttttgct gaaaatggca aagcactggc tgttgggggg attttgggcg
     7921 ttggcgcact gactggctgg cgctactgga acagccatca ggttgattct gcacgctccg
     7981 cttctcttgc ctatcaaaat gcggttaccg cagtgagcga aggcaaaccg gatagcatcc
     8041 cggcggcgga aaaatttgct gctgaaaata aaaatactta tggtgcgctg gcttctttgg
     8101 aacttgcgca gcaatttgtt gacaaaaatg aactagagaa agctgccgcc cagttacaac
     8161 aggggctggc agacacgagc gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc
     8221 gcgttcaggt acagctcaag caggctgatg ccgcgctgaa aacccttgat accatcaaag
     8281 gtgaagggtg ggctgccatt gttgccgact tgcgtggtga agcattgctg agcaaaggtg
     8341 ataagcaagg tgcgcgtagt gcatgggaag caggcgtgaa aagcgatgtt actccggcac
     8401 tgagcgaaat gatgcagatg aaaattaata atttgtccat ctgagaggga cccgatgcaa
     8461 ttgcgtaaat tactgctgcc aggactgctt tccgttaccc ttttaagcgg ctgttcgctg
     8521 tttaacagcg aagaagatgt ggtaaagatg tccccattgc caaccgttga aaaccagttt
     8581 acgccgacca cggcgtggag cacttccgtt ggtagcggca ttggcaactt ctattccaat
     8641 cttcatccgg cactggcgga caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa
     8701 gcgctgaatg cggatgatgg caaagaaatc tggtctgtca gcctggccga gaaagatggc
     8761 tggttctcta aagagcctgc attactttct ggcggtgtga ccgtgtctgg tgggcatgtc
     8821 tacattggca gcgaaaaggc gcaggtttac gcgctgaata ccagcgatgg tactgtggca
     8881 tggcaaacta aagtcgcggg tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg
     8941 ttaatccaca ccagtaacgg tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa
     9001 tggacagtta acctcgatat gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg
     9061 gcttttggtg cggccgtcgt gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa
     9121 cagggccaga tgatttggca gcagcgtatt tcccaggcga ccggttctac cgaaattgac
     9181 cgtctgagcg atgttgacac gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc
     9241 tataatggta acctgacggc gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa
     9301 ctgggttcgg tgaatgattt catcgtcgat ggcaatcgca tctatctggt cgatcaaaat
     9361 gaccgggtga tggcgttgac cattgatggc ggcgttacgc tgtgggcaca aagcgatctg
     9421 ctgcatcgcc tgctgacttc tccggtgctg tataatggca acctggtggt cggtgacagt
     9481 gaaggttatc tgcactggat taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt
     9541 gatagttccg gtttccagac tgaaccggtt gccgctgacg gcaaactgct gatccaggca
     9601 aaagacggaa ccgtgtactc tattacacgt taatcgtctc tgtcgttcac tttgaaaacg
     9661 gctcctggac aggggccgtt ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt
     9721 ctgatgattt ataaaaatga ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc
     9781 cctaacgtag gaaaatccac gttatttaac cgtctaactc gcacccgaga tgcgctggtt
     9841 gcggatttcc cgggtctgac tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt
     9901 gagtttatct gtattgatac cggcgggatt gatggcacag aagacggtgt agaaacccgc
     9961 atggcggaac agtcgctgct ggcgattgaa gaagcggacg tcgtactgtt tatggtggat
    10021 gcgcgcgcgg gcctgatgcc ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa
    10081 aaaccgacct tcctggtggt aaacaaaact gacggtctgg atcccgatca ggcagtggtt
    10141 gatttctact cgcttggttt aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc
    10201 gtattaagtc tgctggagca tgtgctgctg ccgtggatgg aagatctcgc accgcaagag
    10261 gaagtcgacg aagacgctga atactgggcg caatttgaag cggaagagaa cggcgaagaa
    10321 gaagaggaag acgacttcga cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt
    10381 ccgaacgtag gtaagtctac actcactaac cgtattcttg gagaagagcg cgttgttgtt
    10441 tacgacatgc ctggcacgac gcgtgacagc atctacatcc caatggaacg cgatggacgt
    10501 gagtatgtgc tcattgacac cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta
    10561 gagaaattct ccgtaatcaa aacgttgcag gccattgaag acgccaacgt ggtgatgtta
    10621 gtgattgatg cgcgcgaagg tatttccgat caggatctct cgctgctggg ctttattctc
    10681 aatagtgggc gctcacttgt cattgtggtg aataagtggg atggcctgag ccaggaagtg
    10741 aaagagcagg tgaaagaaac gctggacttc cgtctgggct ttatcgattt tgctcgtgtg
    10801 cactttatct ctgccttgca cggcagtggt gttggtaact tgtttgaatc agtacgtgaa
    10861 gcgtatgaca gctccacccg tcgtgtgggg acctctatgc tgacgcgcat catgacgatg
    10921 gctgttgaag atcaccaacc gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc
    10981 cacgctggtg gttataaccc gccgattgtg gtgattcacg gtaatcaggt gaaagacctg
    11041 cctgattcct acaagcgcta cttgatgaac tacttccgca aatcgctgga cgtaatggga
    11101 tcgccgattc gtattcagtt caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc
    11161 ctgacgccaa cccagatgcg taaacgtaag cgtctgatga agcacatcaa gaaaaataaa
    11221 taattatccg taagtatttt atctgccatt ccctctatga atgtagaggg aatggctacc
    11281 ggaggaattc atatggaact gcattgtcca aaatgtcagc atgtgcttga tcaggataac
    11341 ggccatgcgc gttgcccatc ctgcggggaa tttattgaaa tgaaagctct ttgtccagac
    11401 tgccatcaac cgttgcaggt gttaaaagcc tgcggtgcag tagattactt ctgtcagcac
    11461 gggcatggct tgatatcgaa aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta
    11521 cgcgattttt ttaccggctg gatgtttttt acttcacttt ctatccagcc gtcttccaga
    11581 cgtgtggtta gcatttcacc cgttttaact tgcttcactt ttttcagtac attgccgtca
    11641 gtagcagtag taacgctgta tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg
    11701 tgcgttactg cattaccgaa acgttcacgc gtggcgctaa gctgtgcgcg caggatttct
    11761 gctaaacgat attccagttg ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga
    11821 ttttgctgat tcagccgctg tgttaatcgc tgctgctgtt gcccggcacg cttaagctga
    11881 ttttccagcg caaagctcat ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc
    11941 agccggagct gcggatgctg ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgtgta
    12001 cggttggcga gataataatc catcgccatc tccagccgtt gacgggccga ttgcacctgg
    12061 cgcagtaact cttgctgatt acggctcact acttcagcgg cggcagacgg cgttggcgca
    12121 cgcagatcgg caacaaaatc ggcaatggtc acatccgtct catgcccgac ggcgctgacg
    12181 accggaatgc ggctggcaaa aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa
    12241 tcttccagcg aaccgccgcc gcgcccaacg atcaacacgt cgcactcatt gcgctgattc
    12301 gccagttcaa tggcgcgaac aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg
    12361 tagatgatca ccggtagaga aggatcgcga cgttttaaca catgcaaaat atcatgtagc
    12421 gcagcaccgg tttttgaggt gatcacacca acgcaatgcg caggggaggg aagtggtttt
    12481 ttgtattgct gatcgaacaa acattcagcc tgcaacttcg ctttgagctg ttcgtacttc
    12541 tgttgcagca gcccttcacc ggccggctgc atactctcaa cgattatctg gtagtcgccg
    12601 cgcggctcgt agagcgtaat attggcgcga actaaaactt gttgcccatg ctgcggacgg
    12661 aaggtgaccc ggcggttgct gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct
    12721 ttgagtgtaa agtaccagtg accggaagcc ggttgtgtga aattagagat ttcgccgctg
    12781 atccaaacct gtcccatctc atgctcaagc agcagacgaa ccgtttgatt caggcgacta
    12841 acggtaaaaa ttgcagggga ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca
    12901 gcaggttatt cagtctatag taacccgccc ttcggggata gcaagcattt tttgcaaaaa
    12961 ggggtagatg caatcggtta cgctctgtat aatgccgcgg caatatttat taaccactct
    13021 ggtcgagata ttgcccatgc tacgtatcgc taaagaagct ctgacgtttg acgacgttct
    13081 cctcgttcct gctcattcta ccgttctgcc gaatactgct gacctcagca cccagctgac
    13141 gaaaactatt cgtctgaata tccctatgct ttccgcagca atggataccg taacggaagc
    13201 gcgcctggct attgctctgg ctcaggaagg cggtatcggc tttatccaca aaaacatgtc
    13261 cattgaacgc caggcagaag aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac
    13321 tgatccgcag actgttctgc caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg
    13381 taacggtttt gcgggctatc cggtcgttac cgaagaaaac gaactggtgg gtattatcac
    13441 cggtcgtgac gtgcgttttg ttaccgacct gaaccagccg gttagcgttt acatgacgcc
    13501 gaaagagcgt ctggtcaccg tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat
    13561 gcacgaaaaa cgcgttgaaa aagcgctggt ggttgatgac gaattccacc tgatcggcat
    13621 gatcaccgtg aaagacttcc agaaagcgga acgtaaaccg aacgcttgta aagacgagca
    13681 aggccgtctg cgtgttggtg ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga
    13741 cgcgctggtt gccgctggcg ttgacgttct gctgatcgac tcctcccacg gtcactcaga
    13801 aggcgttttg cagcgtatcc gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg
    13861 cggcaacgtg gcaacagctg caggtgcacg cgctttggca gaagctggtt gcagcgcggt
    13921 taaagtcggt atcggccctg gttctatctg tacgactcgt atcgttactg gcgtaggtgt
    13981 tccgcagatc actgccgttg ctgacgcagt tgaagccctg gaaggcaccg gtattccggt
    14041 tatcgcagat ggtggtattc gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc
    14101 aagcgcggtg atggtaggtt ccatgctggc gggtactgaa gaatctccgg gtgaaatcga
    14161 actctaccag ggccgttctt ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc
    14221 caaaggttcc tctgaccgtt atttccagag cgataacgct gccgacaaac tggtgccgga
    14281 aggtatcgaa ggtcgcgtag cttataaagg tcgcctgaaa gagatcattc accagcagat
    14341 gggcggcctg cgctcctgta tgggtctgac cggctgtggt actatcgacg aactgcgtac
    14401 taaagcggag tttgtacgta tcagcggtgc gggcattcag gaaagccacg ttcacgacgt
    14461 gaccattact aaagagtccc cgaactaccg tctgggctcc tgattctctt cgcccgattt
    14521 catgtcgggc gatttatatt atctgtttca cttgcctcgg aataagcgtc aatgacggaa
    14581 aacattcata agcatcgcat cctcattctg gacttcggtt ctcagtacac tcaactggtt
    14641 gcgcgccgcg tgcgtgagct gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa
    14701 gcacaaattc gtgacttcaa tccaagcgga attattcttt ccggcggccc ggaaagtact
    14761 actgaagaaa acagtccgcg tgcgccgcag tatgtctttg aagcaggcgt accgatattc
    14821 ggcgtttgct atggcatgca gaccatggca atgcagttgg gcggtcacgt tgaagcctct
    14881 aacgaacgtg aatttggcta cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc
    14941 ggtatcgaag atgcgctgac cgcagacggt aaaccgctgc tcgatgtctg gatgagccac
    15001 ggcgataaag ttaccgctat cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc
    15061 ccgtttgcca ttatggctaa cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa
    15121 gtgactcaca cccgccaggg tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag
    15181 tgtgaagccc tgtggacgcc agcgaaaatt atcgacgatg ctgtagctcg catccgcgag
    15241 caggtaggcg acgataaagt catcctcggc ctctctggtg gtgtggattc ctccgtaacc
    15301 gcaatgctgc tgcaccgtgc tatcggtaaa aacctgactt gcgtattcgt cgacaacggc
    15361 ctgctgcgtc tcaacgaagc agagcaggtt ctggatatgt ttggcgatca ctttggtctt
    15421 aacattgttc acgtaccggc agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat
    15481 ccggaagcaa aacgtaaaat catcgggcgc gttttcgttg aagtattcga tgaagaagcg
    15541 ctgaaactgg aagacgtgaa gtggctggcg cagggcacca tctaccctga cgttatcgaa
    15601 tctgcggctt ctgcaaccgg taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc
    15661 ctgccgaaag agatgaagat gggcctggtt gaaccgctga aagagctgtt caaagacgaa
    15721 gtgcgtaaga ttggtctgga gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc
    15781 ccgggaccag gccttggcgt tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg
    15841 ctgcgccgtg ctgacgccat cttcattgaa gaactgcgta aagcggacct gtacgacaaa
    15901 gtcagccagg cgttcaccgt cttcctgcca gtacgttccg ttggcgtaat gggcgatggt
    15961 cgtaagtatg actgggttgt ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca
    16021 cactgggcgc atctgccgta cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa
    16081 gtgaacggta tttcccgcgt ggtgtatgac atcagcggca agccgccagc taccattgag
    16141 tgggaatgat tttgatccct gcactatgaa tgaacaaaac cctccgttac tacagagggt
    16201 tttttatctt caagaatcat aggcttgaag ttactaacat cgattaatta aaccagctgt
    16261 ccgatttgtt ctcttctgct ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca
    16321 tatttctgtt tgcgtcagct tcagattgca ccacatcggt ttgcgcagct ttgtgcttca
    16381 gttcctgatc gataaattcg ttttcgcgct taacgcgggc ttcttctttc gccagcgcca
    16441 gtttttgttt ctgaatctct aagctgcgta gctcatcttc ataactttga tcgcgttttt
    16501 tgtccgcagt ggcttcggcg tccagtttat cctgacgagc tttcttattt gccgctgccg
    16561 ttgccgctct tttgttcgcg gcggcctggg catttgcgcg acgttgcttc tcttgctgga
    16621 tttccctgtt gcgctccgcg acccattcgt catgctgcct ttgctcttca tttttacctt
    16681 gctgttccgc ttctgcgaca gcagagagtt gatcctgcaa tgatgaggct atagccggat
    16741 agcttaagga ggctaagatg gcgcaaagaa aaactttctt catgactcct cctgattatt
    16801 agctcttttc aggacatttg gtatttggct gaatacgcgt ttcgttatac gttgtggtaa
    16861 taacaacggc taaacctgtc gtaaactggc actctttacc ctcctgggtg gaggtataca
    16921 ctttggtgcc ttccttatat gttaaagaaa caccttccac taaggtttta tcattcacca
    16981 tagaacccgc tgccgcgcct acagctccgc cgccaactgc ccctgccgtc gttccggaat
    17041 tgctgccaga accgacgttg tggccgataa caccgccagc gactgcgcca ataagcgcgc
    17101 cgaaggcttg tgcgttccgt ttattttggg agttgtctac ggcaactttt gcgggaagaa
    17161 tggaaataat attaacggtt ttagtttctt gtttggtatt cagttgatcg gtttgataaa
    17221 catcggcggc atgatcgtca gcatttgact ggcatcctgc cagagtgaat gacgctaaca
    17281 ttgccacagg cagaagacat tttttaaatt tcatcactat tccttgttat acacattacg
    17341 aaaaataaga catttaagat catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat
    17401 agattaaatt tattaaaatt cacgcaggga ataatttata aaaatatatc attacaacat
    17461 atatattaat tttatattta tttcgcagcc aaacaggata aacatcagtc aggacaaaga
    17521 cgtaaaattt aagatgaaag aatatctgcc gctttgataa tcccaaagag caaaagcatc
    17581 cgacagcgaa gtgccgttat gttctgtgaa agtggagaag gtaaccgcta acgttgtacc
    17641 ggtgattcga cggagagtgt ggattgctga gcttcttttt tgctctgatg gtgctgccag
    17701 gcaccgacgg acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg
    17761 atacgggtca tgcgactgtt aaatcggtgt cgttttcgca tactagttgc ctgactcaca
    17821 aaaggttcct tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga
    17881 caaaatggtc aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat
    17941 ggaagttaat ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg
    18001 taataattca ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga
    18061 cacttagaat caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg
    18121 cctgtctgaa tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga
    18181 atgcaactta tataaaaata cgtgataaat ggtgggggct tccgctgttc ctgccttctt
    18241 taatcttgcc cattttcgcc cacattaata ctttcgcgca tatttcttcc ggtgaggttt
    18301 ttctctttta tctgcctttg gcactgatga tcagcatgat gatgtttttc agctgggcgg
    18361 cattgccagg gatcacctta gggatttttg tccgcaaata tgcagagctg ggtttttacg
    18421 aaacgctctc attaacggct aattttatta tcattatcat tctctgttgg ggcggttaca
    18481 gggtctttac tccccggcgt aacaacgttt cacatggtga tacccgttta atttcccagc
    18541 gtatattctg gcagatagtg tttcctgcaa cgctgtttct gatacttttc cagtttgctg
    18601 catttgtagg attactggcg agcagagaaa atctggtcgg tgtcatgcct tttaacctcg
    18661 ggactttaat caattatcag gccttactgg tgggtaatct gatcggtgtc ccgctgtgct
    18721 acttcatcat tcgggtagtg cgaaatccat tttatttacg tagctattat tcgcaattaa
    18781 aacagcaggt tgatgccaaa gtcaccaaaa aagagttcgc gctctggcta ctggcattag
    18841 gtgctttatt gttgctgtta tgcatgccgt taaatgaaaa aagcacaa
//
LOCUS       DAJTRN010000079        18052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRN010000079 DAJTRN010000000
VERSION     DAJTRN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            446..1129
                     /locus_tag="OO328_002842"
     CDS             446..1129
                     /locus_tag="OO328_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5931342.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO328_002843"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO328_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO328_002844"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO328_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5931343.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO328_002845"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO328_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5931344.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO328_002846"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO328_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5931345.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO328_002847"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO328_002847"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5931346.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO328_002848"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO328_002848"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5931347.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO328_002849"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO328_002849"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5931348.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO328_002850"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO328_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5931349.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO328_002851"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO328_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5931350.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO328_002852"
     CDS             complement(13386..13817)
                     /locus_tag="OO328_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5931351.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO328_002853"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO328_002853"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5931352.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO328_002854"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO328_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5931353.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..17862
                     /locus_tag="OO328_002855"
     CDS             16903..17862
                     /locus_tag="OO328_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5931354.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17849..18052)
                     /locus_tag="OO328_002856"
                     /pseudo
     CDS             complement(17849..18052)
                     /locus_tag="OO328_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4548 a         4664 c         4674 g         4166 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17761 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17821 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17881 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17941 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    18001 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg ca
//
LOCUS       DAJTRN010000080        17796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRN010000080 DAJTRN010000000
VERSION     DAJTRN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            246..770
                     /locus_tag="OO328_002857"
     CDS             246..770
                     /locus_tag="OO328_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5931355.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            851..1525
                     /locus_tag="OO328_002858"
     CDS             851..1525
                     /locus_tag="OO328_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5931356.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1541..4003
                     /locus_tag="OO328_002859"
     CDS             1541..4003
                     /locus_tag="OO328_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5931357.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4019..5052
                     /locus_tag="OO328_002860"
                     /pseudo
     CDS             4019..5052
                     /locus_tag="OO328_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5134..5472)
                     /gene="rcnB"
                     /locus_tag="OO328_002861"
     CDS             complement(5134..5472)
                     /gene="rcnB"
                     /locus_tag="OO328_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5931358.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5691..6521)
                     /gene="rcnA"
                     /locus_tag="OO328_002862"
                     /pseudo
     CDS             complement(5691..6521)
                     /gene="rcnA"
                     /locus_tag="OO328_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6643..6915
                     /gene="rcnR"
                     /locus_tag="OO328_002863"
     CDS             6643..6915
                     /gene="rcnR"
                     /locus_tag="OO328_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5931359.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7007..7103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7138..7926
                     /gene="thiM"
                     /locus_tag="OO328_002864"
     CDS             7138..7926
                     /gene="thiM"
                     /locus_tag="OO328_002864"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5931360.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7923..8723
                     /gene="thiD"
                     /locus_tag="OO328_002865"
     CDS             7923..8723
                     /gene="thiD"
                     /locus_tag="OO328_002865"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5931361.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8788..9609
                     /locus_tag="OO328_002866"
     CDS             8788..9609
                     /locus_tag="OO328_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5931362.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTEKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9657..10403
                     /locus_tag="OO328_002867"
     CDS             9657..10403
                     /locus_tag="OO328_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5931363.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10377..11342)
                     /locus_tag="OO328_002868"
     CDS             complement(10377..11342)
                     /locus_tag="OO328_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5931364.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11339..12342)
                     /locus_tag="OO328_002869"
                     /pseudo
     CDS             complement(11339..12342)
                     /locus_tag="OO328_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12382..13434
                     /gene="fbaB"
                     /locus_tag="OO328_002870"
     CDS             12382..13434
                     /gene="fbaB"
                     /locus_tag="OO328_002870"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5931365.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13742..14596
                     /gene="gatY"
                     /locus_tag="OO328_002871"
     CDS             13742..14596
                     /gene="gatY"
                     /locus_tag="OO328_002871"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5931366.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14625..15887
                     /gene="gatZ"
                     /locus_tag="OO328_002872"
     CDS             14625..15887
                     /gene="gatZ"
                     /locus_tag="OO328_002872"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5931367.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15897..16349
                     /gene="gatA"
                     /locus_tag="OO328_002873"
     CDS             15897..16349
                     /gene="gatA"
                     /locus_tag="OO328_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5931368.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16380..16664
                     /gene="gatB"
                     /locus_tag="OO328_002874"
     CDS             16380..16664
                     /gene="gatB"
                     /locus_tag="OO328_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5931369.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16668..>17796
                     /locus_tag="OO328_002875"
     CDS             16668..>17796
                     /locus_tag="OO328_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5931370.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIG"
BASE COUNT         4664 a         4049 c         4504 g         4579 t
ORIGIN      
        1 ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa tctacattaa
       61 ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat attgcattgg
      121 ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt tttgatgtga
      181 gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt attaaggaac
      241 atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt atgagcgctg
      301 gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat attgcagaat
      361 ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg actgtaccaa
      421 ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc ggtgtaacca
      481 gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc tcgttgatca
      541 caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat accgtaggtg
      601 ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat aaaacagcgg
      661 aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat aactcagtgc
      721 aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa taaataaaat
      781 tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt ttaatttaag
      841 aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc ttcctgcaca
      901 tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata aagaagttat
      961 ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga ttgatgatgg
     1021 cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc caccagtggc
     1081 aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga ataaactgcc
     1141 cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa atagtccaga
     1201 gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt tgttttaccg
     1261 gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg taaatcgcag
     1321 tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt cggatgtcaa
     1381 agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag aaagtcagag
     1441 tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca atgaccacgg
     1501 caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa tgaccccact
     1561 tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg aagaaacctt
     1621 tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata ttcgtcttga
     1681 tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata agcaatggcg
     1741 cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat caagagaagt
     1801 tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat gtttaacatt
     1861 tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc gtctcgattt
     1921 cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac cggaaaactg
     1981 ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt acagcgacta
     2041 taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt taaatttact
     2101 ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc caggggtgtg
     2161 gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca cgcttcgcgt
     2221 gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg gtgtgcggtt
     2281 gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac gggtgcaggg
     2341 gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg tttatcagaa
     2401 agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg gtggagcaga
     2461 tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg tgccttatgc
     2521 agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg cgggggcgag
     2581 caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact tattgacgct
     2641 gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa cgggttggaa
     2701 cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac aagacaacgg
     2761 cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga gccaaacgtc
     2821 gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga catttaacga
     2881 tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata tctatgacat
     2941 tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca atatgagcca
     3001 gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag attactgggg
     3061 gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc ggcggataag
     3121 ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga aacgttttaa
     3181 tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc gtcggcaaat
     3241 atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata atacgggatt
     3301 atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga gtcatcaaca
     3361 tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg ttgcgacagt
     3421 gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg tttcaggggg
     3481 cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa cgtttgctgt
     3541 gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc gcacaacaaa
     3601 ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc acctgatgct
     3661 ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa ttgccgcccc
     3721 ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc cctggtttat
     3781 aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca atgatatcca
     3841 tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca ccaatgaaat
     3901 accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca caatcacctt
     3961 cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag gtggttctat
     4021 ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga gctatgcggc
     4081 atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt ttgcacttac
     4141 tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg tgagtacaaa
     4201 tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg ataccattaa
     4261 attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa ataatcagag
     4321 cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg caaaagtgaa
     4381 tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat tatccagtgc
     4441 ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat taggtggcaa
     4501 gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg caggtgtcta
     4561 ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac aggacgattt
     4621 attaattact ttagacagta ttcccgtttt acaattacca gccacaggta acaaagcaac
     4681 aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag gtcaacaaaa
     4741 tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa ccaacagcaa
     4801 cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag taatggttcg
     4861 ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt aaaagaaatt
     4921 gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc cagttactac
     4981 gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt aatcaacgtg
     5041 aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat aacaaggtgt
     5101 ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa tctcaccgtc
     5161 gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc cacccatata
     5221 ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact gcttaatgtt
     5281 gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat gcttaaagtc
     5341 agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg aacccgcggt
     5401 ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca acatcttatt
     5461 tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat atctttcctg
     5521 tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc gtaaaaagtg
     5581 taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg aaatagctga
     5641 tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag ttatcgcatt
     5701 atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa cagactggaa
     5761 aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc gacctgctga
     5821 acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc aatgctgaaa
     5881 ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat caacagcacg
     5941 gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa aatttgccag
     6001 ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc tcgtgcatgg
     6061 gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg gtgatgttcg
     6121 tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc cagccagttg
     6181 cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat gatcactgcg
     6241 gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact gatcaccatc
     6301 ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc cagtccgagc
     6361 atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat catcgttttt
     6421 gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg gatgaagaac
     6481 caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat tcttagtatt
     6541 aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc agtagattaa
     6601 tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca tacaatccgt
     6661 gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt ggcgctcaag
     6721 aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc tgctatccgt
     6781 ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga acacatcgtt
     6841 caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa ggtgctggat
     6901 tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct cgtttacaac
     6961 gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa acgactcggg
     7021 gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat aatgccagcg
     7081 tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga gcaaactatg
     7141 caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt tcaccaacat
     7201 tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc caataccttg
     7261 ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag tcagtttgcg
     7321 gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg cgcacaggcg
     7381 atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct tgatccagta
     7441 gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc ttttaaaccg
     7501 gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc taatggcgga
     7561 cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca aacactggca
     7621 cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac cgatggacat
     7681 cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg aactggctgt
     7741 gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct ggaaaatgtc
     7801 gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag aagcgagggg
     7861 ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca ggaggtgcag
     7921 gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt ggtgcgggga
     7981 ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca gttattactg
     8041 cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag cctgattttg
     8101 tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact aaaatcggta
     8161 tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt tatcagatcc
     8221 aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg ctttcacctt
     8281 cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata acgccaaact
     8341 tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag gaaatgctgg
     8401 aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa ggtggtcatc
     8461 tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa cggtttaccg
     8521 caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct gcggcgttgg
     8581 ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca aaaatctggc
     8641 tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt ggtccggttc
     8701 accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat tgtcagcctt
     8761 tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg aaaaaaactc
     8821 accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc gcgtcagacg
     8881 gtgaattttt tctattcgac agcagttcag attacagact acatccactt ctacggttat
     8941 cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca cggattagat
     9001 gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg cgacaacgat
     9061 atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt ggacccctat
     9121 ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc gtatcattat
     9181 ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac ggtggatttc
     9241 tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt atcggcaaaa
     9301 gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac ggaaaaaaag
     9361 ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta tttcaatgaa
     9421 tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg catggcctgg
     9481 cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc ggtggatttt
     9541 aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt aaagaaacgc
     9601 cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa agacaaatgg
     9661 aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg gcggataaca
     9721 cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc ggggtgctgg
     9781 agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc gtttcgcgca
     9841 ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg cgttcgcgcg
     9901 gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg agaggttttt
     9961 ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat aagcgtgtcg
    10021 tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt gatgtctttt
    10081 tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa tcgtgggttc
    10141 cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac tatttccgca
    10201 gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg ccggatgccg
    10261 agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc aagcaagtgg
    10321 cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc agcgatctat
    10381 acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc gccccgatgc
    10441 ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag tggcagacca
    10501 gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat ggtgtctaca
    10561 acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata ccatgcgcct
    10621 tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg cttgccaagt
    10681 gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc ttgacgattg
    10741 agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg gatatcgcca
    10801 atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag ccattccact
    10861 aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag gttgccaggc
    10921 gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc gctgaatgac
    10981 ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt atcgctttcg
    11041 gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat aatctccgcc
    11101 cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg ctttaacgcc
    11161 acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag ttcgatatcg
    11221 cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc cgcgccgacg
    11281 accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg agcgccgctc
    11341 ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg ttgaaatcaa
    11401 gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta atgccatgca
    11461 acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg ccaagattgg
    11521 cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc gcaatggcgc
    11581 acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc tgttcgcggg
    11641 cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga cgtgccgcca
    11701 gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt gaaggcaatg
    11761 aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc catgcgatga
    11821 ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc agcgccacat
    11881 catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc gagacgcgca
    11941 tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta ccgtcgcgaa
    12001 tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag gcgtcgaaac
    12061 gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg ccttcacatt
    12121 ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt cggcgcggtt
    12181 aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa tccagccaaa
    12241 gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg catcccctaa
    12301 cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc cccttgaatc
    12361 ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac
    12421 aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct ccccggacat
    12481 gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt acgtaatatg
    12541 cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt
    12601 gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac
    12661 ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc atcaacttac
    12721 ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct cgtcaaactt
    12781 aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg
    12841 gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt tggctcggaa
    12901 gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca cgagctgggc
    12961 atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga tggcgttgat
    13021 taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac cataggtgca
    13081 gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat taattacggt
    13141 tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt
    13201 tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg cggtgctgca
    13261 ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa acgcgcaggc
    13321 ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa
    13381 ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc ctgatacgcc
    13441 tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat ccggcaacct
    13501 atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca cgtatgttta
    13561 caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt
    13621 tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc attatctcga
    13681 catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac aagatatgaa
    13741 aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt
    13801 tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa
    13861 cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg ctggtacaga
    13921 aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca
    13981 tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc
    14041 agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt
    14101 ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc aacttggcgg
    14161 ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc ccgcacaggc
    14221 gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg gcacggctca
    14281 cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca ttcgccagtg
    14341 ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg atattcagca
    14401 aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctt
    14461 gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc gggattattt
    14521 gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg
    14581 cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg
    14641 cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg
    14701 ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg attgaagcaa
    14761 cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg
    14821 aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt attctcggcg
    14881 gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat
    14941 ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt
    15001 caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg gaacgagctg
    15061 ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag caactgagct
    15121 atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt cagtcagtac
    15181 acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag gcctttattg
    15241 cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag ccgggtgtgg
    15301 aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggca ctggcgcaat
    15361 ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag acccggacgg
    15421 cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc gcattaacct
    15481 ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt atcgcccccg
    15541 aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa ccgcaatact
    15601 ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt tacagcctgt
    15661 cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt gaaacgatga
    15721 tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat cttcccaaac
    15781 aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt attatgaata
    15841 aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga cggtatatga
    15901 ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt ttaacctata
    15961 tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag gcattaattg
    16021 ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc
    16081 attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag
    16141 ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt attgcgttga
    16201 ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac
    16261 agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag gaatacttca
    16321 caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta
    16381 tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg
    16441 aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc cagtgtcggg
    16501 ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact gccagggtgg
    16561 atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct ggtgtcggta
    16621 tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg ttttcagaag
    16681 tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc atcattattt
    16741 tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg catatcggga
    16801 ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt ggtccggcgg
    16861 cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg
    16921 gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg attgcgatat
    16981 tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga
    17041 atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt tcatggatga
    17101 tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc gactggtttg
    17161 cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac ggtacgtcgg
    17221 cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta
    17281 accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt ggcgaacctg
    17341 tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac gatgtcaaag
    17401 gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca cggatgatta
    17461 aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga
    17521 agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg gggcatacgg
    17581 cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct gtttgtgttc
    17641 cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc gtggcgatgg
    17701 cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc atcattatga
    17761 gcatcaccct gtggatcgcg acgcaaacta ttggtt
//
LOCUS       DAJTRN010000081        17597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRN010000081 DAJTRN010000000
VERSION     DAJTRN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..673)
                     /gene="chbR"
                     /locus_tag="OO328_002876"
                     /pseudo
     CDS             complement(1..673)
                     /gene="chbR"
                     /locus_tag="OO328_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(681..1031)
                     /gene="chbA"
                     /locus_tag="OO328_002877"
     CDS             complement(681..1031)
                     /gene="chbA"
                     /locus_tag="OO328_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5931371.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="OO328_002878"
                     /pseudo
     CDS             complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="OO328_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="OO328_002879"
     CDS             complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="OO328_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5931372.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="OO328_002880"
     CDS             complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="OO328_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5931373.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3681..4508
                     /gene="nadE"
                     /locus_tag="OO328_002881"
     CDS             3681..4508
                     /gene="nadE"
                     /locus_tag="OO328_002881"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5931374.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4738..5625
                     /gene="cho"
                     /locus_tag="OO328_002882"
     CDS             4738..5625
                     /gene="cho"
                     /locus_tag="OO328_002882"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5931375.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5585..6160)
                     /gene="ves"
                     /locus_tag="OO328_002883"
     CDS             complement(5585..6160)
                     /gene="ves"
                     /locus_tag="OO328_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5931376.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6363..6848)
                     /gene="spy"
                     /locus_tag="OO328_002884"
     CDS             complement(6363..6848)
                     /gene="spy"
                     /locus_tag="OO328_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5931377.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7178..8146)
                     /gene="astE"
                     /locus_tag="OO328_002885"
     CDS             complement(7178..8146)
                     /gene="astE"
                     /locus_tag="OO328_002885"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5931378.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8139..9472)
                     /gene="astB"
                     /locus_tag="OO328_002886"
                     /pseudo
     CDS             complement(8139..9472)
                     /gene="astB"
                     /locus_tag="OO328_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9469..10947)
                     /gene="astD"
                     /locus_tag="OO328_002887"
     CDS             complement(9469..10947)
                     /gene="astD"
                     /locus_tag="OO328_002887"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5931379.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10944..11978)
                     /gene="astA"
                     /locus_tag="OO328_002888"
     CDS             complement(10944..11978)
                     /gene="astA"
                     /locus_tag="OO328_002888"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5931380.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11975..13195)
                     /gene="astC"
                     /locus_tag="OO328_002889"
                     /pseudo
     CDS             complement(11975..13195)
                     /gene="astC"
                     /locus_tag="OO328_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13641..14447
                     /gene="xthA"
                     /locus_tag="OO328_002890"
     CDS             13641..14447
                     /gene="xthA"
                     /locus_tag="OO328_002890"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5931381.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14614..15327
                     /locus_tag="OO328_002891"
     CDS             14614..15327
                     /locus_tag="OO328_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5931382.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLLYARHK
                     HVDLSASRRSPLTHPKNEG"
     gene            15332..16009
                     /gene="ydjY"
                     /locus_tag="OO328_002892"
     CDS             15332..16009
                     /gene="ydjY"
                     /locus_tag="OO328_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5931383.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16024..16731
                     /locus_tag="OO328_002893"
     CDS             16024..16731
                     /locus_tag="OO328_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5931384.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16731..17279
                     /locus_tag="OO328_002894"
     CDS             16731..17279
                     /locus_tag="OO328_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5931385.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17289..>17597
                     /locus_tag="OO328_002895"
     CDS             17289..>17597
                     /locus_tag="OO328_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5931386.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     N"
BASE COUNT         4350 a         4676 c         4299 g         4272 t
ORIGIN      
        1 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
       61 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
      121 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      181 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      241 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      301 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      361 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatgcgcgtg gcaccaaact
      421 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
      481 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
      541 aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata aacacatgga
      601 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
      661 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
      721 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
      781 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
      841 gcttcattca atgccatccg tgactgatcc atcatggctt tcgctgcggc aaaatcgccc
      901 tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg cttgtccaga gttgatgata
      961 agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg aatattatcg
     1021 agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt accggcatcg
     1081 attaaaattt caggggttag cgatatcttc ttcgctctct tctttatcaa tcgcattttg
     1141 tgctttgtta gccaccacaa caaagggcag ataaattaac gttgcgatgc caaggttgaa
     1201 gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta aagaaggctc ccagaccggt
     1261 tggcatggtc cacggtgcaa tattggtcac cggaggaatg atgcccatgt agtacgctgc
     1321 gagggtgatt gccgccagaa tcggttgtac cagtacaaac gggataaaca tcaccgggtt
     1381 catgataatt ggcagaccaa acagaatcgg ttcgttaatc tggaagatgc cggacggcag
     1441 cgccagtttt gccacctgac gataatcagc acggcgagag gcgataaaga tagccaggat
     1501 caggcctaaa gtcgcaccac tgcccccaag gaaaataaag gagtccagca tcggcttggc
     1561 ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat attgctgata
     1621 ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg cggtcagcgc
     1681 cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat aggcccagcc
     1741 caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga tctgatggaa
     1801 gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa gaataataaa
     1861 gccgggaatt aatgccgaga aagaacgcga tactgaagca ggtacgctgt cgggcagttt
     1921 aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca ggccaataat
     1981 aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat aggcctcacc
     2041 gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccag ccgccagcgc
     2101 atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca ttaaagacat
     2161 tattcctaat gttccgttat atacgttgcc gccaatacct ttcagaccat taagtgtttc
     2221 aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa acgacccctc cccaaagctt
     2281 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     2341 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     2401 aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaaaaaccg
     2461 caatttaaat attacggtat tgatttatga aataactctt tgacgggaaa atttaaaata
     2521 aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca
     2581 tcgactttgc cataaagcag cgagtcaatt acttcaaccg gtttgttggg taacaaacgc
     2641 tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga
     2701 cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt
     2761 tctgcctgtg cgcgcatttt tgataccagt aaagagatag acatgcccgc agaacaaaac
     2821 agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac
     2881 actccgcaag ccttgacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt
     2941 ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttat
     3001 aaattgtttc tccttgacct gtggttatga ccctcttcac attcgggcaa ataattcgcg
     3061 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
     3121 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
     3181 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
     3241 gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg
     3301 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     3361 ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata agctgtacaa
     3421 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     3481 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     3541 agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     3601 tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct
     3661 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     3721 aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc
     3781 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     3841 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa actcgcaacg
     3901 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggctgac gaacaagatt
     3961 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     4021 cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     4081 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     4141 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     4201 atggtgacgg cggtacggat attaatccgc tgtatcgtct caacaaacgc cagggtaaac
     4261 agttactgac ggcattaggt tgcccggaac acctttataa gaaagcccca actgccgatc
     4321 ttgaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     4381 tcgacgacta tctggaaggg aaaaacctac ctgaacaggt cgccagaaca atagagaact
     4441 ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa cgttttcgat gatttctgga
     4501 aaaagtaata atttgcacct attggattgt gcgaaaaaga gtaatttgtt cacgccggat
     4561 gcggcgtgaa cgccttattc tacctacaaa actatgcaaa ttcaatacat tgcaggagtc
     4621 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatca acccgcctct
     4681 tttttaatcg cctcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     4741 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     4801 gaacatttac attcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     4861 ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg
     4921 ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc
     4981 tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     5041 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     5101 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     5161 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag catcgctgat
     5221 gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagtcgcgg tcgtgcatgt
     5281 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     5341 catgcgctac gcttgcgcca gtctctggag cgtttgcgag tggtgtgttg gccttggcaa
     5401 ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     5461 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     5521 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     5581 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     5641 gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag cacacgccct
     5701 ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     5761 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     5821 catccaggcg cgtcataata ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt
     5881 ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc
     5941 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     6001 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     6061 aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc
     6121 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     6181 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     6241 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     6301 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     6361 gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc
     6421 tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc
     6481 tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc
     6541 gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt
     6601 tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc gtggatctgc
     6661 tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac
     6721 ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg
     6781 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     6841 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     6901 cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     6961 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga
     7021 gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga actataccgg
     7081 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     7141 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     7201 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     7261 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca
     7321 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt
     7381 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     7441 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     7501 gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc aaaatgtctg acgctgaaat
     7561 gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     7621 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     7681 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccaacgcaca gattctttgc
     7741 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     7801 tttcagcaaa tagctgccaa cgaccgccaa acattcggtt catatcgcta tggcaataac
     7861 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     7921 ggatctcgcc gtgagatatc gcgccaagca acgcgtccag catttccaca ggtgccgtct
     7981 cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca
     8041 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     8101 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     8161 cgctggaacg gataaaccga accgagattc agtaattggc tcaatacatc cagtgcttcc
     8221 cgcccttcgc gcagcaattg cgggtcggcc agatcggcag cagtaagacg atcgcggtag
     8281 taacgatcca cccagtcatt gagcgcatta aacaaggtat cgttcatcat caccgccgga
     8341 ttcaccgccc ggcgttcttc ttctgtcaat accacccgca accgcaggca cgccgggccg
     8401 ccgccattcg ccatgctttc acgtaagtca aagactttta gttcgctaat cgggttgtca
     8461 gcggcaagga gttcattgag ataaccccat actccggcgt gttcccgaca ctcctgaggc
     8521 agcaccaaca tcatggaacc atcatcgcgg ctcaacagtt ggctgttaaa cagataggta
     8581 gacaccgcat ccgacacgga aacctgagtt gccggaactt ctatcgccat aaaaccgttc
     8641 acccgcgtgc cagtaactgt gactggcgag cgaacgcctg ttggtggcaa aacagcacct
     8701 ggcggttact cacggcaatc acgtcattat gaaaaacgct ctggtcgata acgtccgggt
     8761 tttgctgggc gaaaatcacc tgttggggat tcacctgatt aagccttgcc accgcctcgc
     8821 tggcttcgcg agtctgtcgc gccggataac gggaaggccg ggtatcattg ccttcttctc
     8881 gcccgtagac aaaaagtagc atacccggtt caccgtaatg accgccgaga cgattgtggt
     8941 ttgccgcccc ctcatcaccg agcaacgcta cctgcggcaa cgccgaatgg acgctaaatt
     9001 tctcttcgtc cttaaaaatc gcttttaaca gcgattcagt gacgggcgct tccagcgaac
     9061 ggtgaaattt attgttcagg ttggcaacgg tgagatgcac tttgccatcc agcgtatcgg
     9121 cagatggcgc gatcgttgcc gcattggcta cccacattgg cgaagcggaa ctgacgctgg
     9181 aaagccagtg cggtgcctgg cgtgcaactt tttccagtac ctgctcatcg ctaccactga
     9241 atcccaactg acgcagcacc ggaataaacg gacgctcgtg cggcggaatc acggcctggg
     9301 ggaatcccgc atcggcaagg gctttcattt tcagtaagcc ctgttttgcc gccagtcgcg
     9361 ggttagacac ctgaaaacgg tgacgggtag aggcttcatt accaaacgac aggcccgcgt
     9421 aatgatgcgt cagccctacc agcccgtcga aattaacttc ccaggcgttc atcgcaccac
     9481 ctcatcggaa aaatccagcc cggggttaag cgtggcgggc aatgttaacg agtccgactc
     9541 caggctcgcc ataggccatg cgcagtaatc tgcggcatac caggcgctgg ggcgatggtt
     9601 accggaagca ccaatgccgc cgaatggggc ggtactggca gcaccagtaa gcggtttgtt
     9661 ccagttaaca atccccgccc gcgcctccag caacagttga tcgaattttt cccgctcggg
     9721 ggaaaccaga ccgcaaaaga gtccgaagcg agtgttgttc gccatccgaa tcgcctcatc
     9781 aaaagtatca taacgccaga cgcgcaaaaa cggcccgaac acctcttcat ccggcacacc
     9841 gccaacgccc gtcatttcaa tgatccctgg tgtcagcaac gatgtccctg cttgtaataa
     9901 gcgcggcgca agcagggttc gtccgcccat cgcttccagt tgctgccagg cagtaaccac
     9961 ctgctgtgcg gcctgttcag aaatcagccc gccaataaac ggctgcggtt cgtcatccca
    10021 gttgcccggc gttaatcgct ggctgacggc aaccagacta gcaagaaacg catcgccctg
    10081 cgctccgctt ttgagcaata aacggcgggc gcaggtgcag cgttgcccgg ctgtgacaaa
    10141 cgccgactga atggtcagat ggacagccgc gtcgatatcc gccacctcat cgataattag
    10201 cggattatta ccgcccatct caagggcgag aattttctcc ggctgaccgg agagctggcg
    10261 atgcagctgg tagccggtat tggcgctacc ggtaaacagc aaaccgtcga gatcctccag
    10321 cgcactcagc gcctgacccg tttcacgccc gccctgcacc aggttcagca cgcccggcgg
    10381 caagccagcc tgctgccata aacgcattac cgcttcgcca ctccacggtg tcagttcgct
    10441 gggtttaaag ataatggtgt tacctgccag caatgccgga acgatatgtc cgttcggcaa
    10501 atgaccaggg aaattatacg gcccaaacac cgccagcacg ccgtgcgggc gatgtcgcag
    10561 gctcgccgca ccgtccggca tttcactacg ctgctcgccg gtacgaacgt gatacgcctt
    10621 aattgatatc gcgattttat tgatcatcgc cgtcacttcg gttgccgctt cccagcgcgg
    10681 cttacccgtt tctctggcaa taatcgcggt taattcggct ttattgcttt ccagcaatcc
    10741 ggcaaagcgt tcgacaacga cctgacgttc agccaatgag agccgcgccc agcgcggaaa
    10801 cgccgcacgg gctgcccgac aagcctgttc gacctgagcg gcatcggcat cattgccttg
    10861 ccataacacc tcgcccgata ccggattacg cttcacacgc gatgcgccct ggcccgttat
    10921 ccagtcaccg ttaatccata aagtcatgct gttttctcct ctgcgcacag gcgcaccaga
    10981 cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt gtgcggcggt taaaatcaaa
    11041 cgctcggttg ccggatcggt acgcgccagc accacgcgga aatggtgata attttcattg
    11101 gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc cttctgccac ttccaccagc
    11161 cgacttttac ggatggcgcg cacgcggtcg atgtcacact caagcgttgg cccgccgtcg
    11221 aagatgtcga tatagttgcg gtaacgaaaa ccttctttct ccagcaccgc gcgggcaggc
    11281 gcggtttgcg gatgtacctg accgatgacg tcctgggctt cctgggataa aaagtgggta
    11341 tagatcggat gtttcggcat cagttctgca ataaatgcct tttgcccggt gccgcagaga
    11401 aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac cgaggctttg ccagaacggt
    11461 gaatagccgt gttcgtcaat caccccgcgc atttcagcaa ccactttgtc attaaacttg
    11521 tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca gcaaatagcc gttgccctct
    11581 ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt gtgatcgtta
    11641 ctaagaaaca gcgtcggcaa tgcgttatag acattcagct cttttgaggc gtgaaccaac
    11701 gtgccgacgc gatagttgta ccagggatcg ttcagcccaa ccgccacctc aatagcgcaa
    11761 atccccgcca cggtgcctgt ctcgctatct tccagcacga acacatagcc ctgctcgctt
    11821 ttgggcagtt caccttgcca ggttttgatt gccctttcga tacgcgccga aagcgtggct
    11881 tcattggcgg gaagcgacgt caggccgccg cccgttttgc tggcaagctg catcagcgcc
    11941 gagacatctg agcgctcaac gggacggatg accatcatga tgaacctcgg ctaacaaagt
    12001 gttcgcaagc agctgcaaag cgatccagtc cggtcgtcac ctcttcttcg ctgacattga
    12061 gcgcaggcgc aaaacgcacc acgttgccac ccgcaatcag taccatcacg cctgctttcg
    12121 ccgcttccag agagagctgt ttcgcttgcc cggcgtaatc ggcattcagt acacagccaa
    12181 tcaacaaacc taagccgcga acttcactga acaaaccata gcggtgatta atagtattaa
    12241 gacgctcaac aaaccagtcg tgacgctgtt taacgccatt aagcatctct ggtgtgttga
    12301 tgagctccag cactttgcct gctaccgccg aggccagcgg attaccgcca taggtggtgc
    12361 catgagtgcc aacggtcatc acgcgggcgc actcttcggt tgccaacaac gcaccgaccg
    12421 ggaaaccgcc gcccagcgct ttggcggtgg ttaacagatc gggcgtcacg ccgtagtgca
    12481 tataggcata cagctcacca gtgcgcccga cgccggtttg tacctcatca aaaatcagca
    12541 aggcattgtg gcggtcacac aattcacgca gaccttgtaa aaacgcgttg ctggctggca
    12601 ccacaccgcc ttcgccctag atgggctcga caatcaccgc acaggtggcg tcgtcaatca
    12661 gcgcgctggc agagttaata tcgttatatg cagcatgacg aatatccgcc ggcagtggcg
    12721 caaaatcctg tgaataggct ggctgcccac ccgcactgac agtaaacagc gtgcgaccat
    12781 gaaacgcatt tttgaacgcc acgatgccac tcttatggct gccgtagcgg tcgtgagcga
    12841 atttacgcgc cagttttagc gccgcttcgt tggcttccgc accggagtta caaaagaaga
    12901 cgcgatcggc aaacgtggcg tcgatcaatt ttttcgccag tcgcagtacc ggctcgttgg
    12961 tgtaaccgtt gccggtatgc cagaacttac tcgcctgttc gttcagcgct tcacgcagtt
    13021 ccggatgcgc atggcccagc gcgttcaccg caatgccacc cgcgaagtcg atatactctt
    13081 tcccctgctg atcccacaag cgcgaacctt cgccacgtac cggtataaag ggtgccggag
    13141 cgtaaacagg tatcatccat tcatcaaagt tttcacgcgt aattggctga gacatagcga
    13201 cctctacagt aaataattcg ttatttgtat gttaacaata agtaatgttt gcgctgtaaa
    13261 tgtagattgc agggttcgtg ccagccagtg ataaaagtgc ataaacggcg gaggctaact
    13321 ggaaatcaag gagttataac caaaccatat gcatttaaag tgcatataaa gtgaatacgt
    13381 ttgcgatgtg ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag taaaatataa
    13441 ttctagaatt gtgatcattg acgaaattta ctggaaatta ctgcgccatt ctgacgcagc
    13501 gcgcaccaaa agcgggcatt ttttgcgcca tcgttgacat cattaacaac catcgatcaa
    13561 atcacttaac aacaggcggt aagcaacgcg aaattctgct accatccacg cactctttat
    13621 ctgaataaat ggcagcgact atgaaatttg tctcttttaa tatcaacggc ctgcgcgcca
    13681 gacctcacca gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg
    13741 agacaaaagt tcatgacgat atgtttccgc tcgaagaggt ggcgaagctc ggctacaacg
    13801 tgttttatca cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa gagacgccga
    13861 ttgccgtgcg tcgcggcttt cccggtgacg acgaagaggc gcagcggcgg attattatgg
    13921 cggaaatccc ctcactgctg ggtaatgtca ccgtgatcaa cggttacttc ccgcagggtg
    13981 aaagccgcga ccatccgata aaattcccgg caaaagcgca gttttatcag aatctgcaaa
    14041 actacctgga aaccgaactc aaacgtgata atccggtact gattatgggc gatatgaata
    14101 tcagccctac agatctggat atcggcattg gcgaagaaaa ccgtaagcgc tggctgcgta
    14161 ccggtaaatg ctctttcctg ccggaagagc gcgaatggat ggacaggctg atgagctggg
    14221 gggtggtcga taccttccgc catgctaatc cgcaaacagc agatcgtttc tcatggtttg
    14281 attaccgctc aaaaggtttt gacgataacc gtggtctgcg catcgacctg ctgctcgcca
    14341 gccaaccgct ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatt cgcagcatgg
    14401 aaaaaccgtc cgatcacgcc cccgtctggg cgaccttccg ccgctaattt agcagctctc
    14461 ctggctcaaa ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact gtcagtaatg
    14521 atttgttgcc tgccgtcctt tgttataccg tcactgcgtt tttagttgtc tgaccacttc
    14581 tctattatca agtttgatat aggaaactcc acgatgaacg ctgagcgtaa ctttctgttt
    14641 gcctgtctta tttttgcgct ggtcatttac gctatccacg ctttcggttt attcgatctg
    14701 ctcaccgatt taccccactt acagacactc attcgccaga gcggattgtt cggttatagc
    14761 ctctatattc tgttattcat caatgccccc ctctttctgt taccaggaag catactggtg
    14821 atcgctggag gaatagtttt tggcccgctc ttagggacac aactctcatt aattgccgca
    14881 acgctggcct cctcgtgctc attcctgctg gcgcgctggc tgggacgtga tttactgctg
    14941 aaatacgtgg gccatagcca taccttccag gccattgaaa aaggcattgc gcgtaacggt
    15001 attgattttc ttattctgac ccgcttaatc ccgttgtttc cttacaatat tcaaaattac
    15061 gcttacggat tcaccaccat cgccttctgg ccttataccc ttatttcggc actcacgacc
    15121 ctgcccggta ttgttattta taccgtgatg gcaagcgatc tcgccaatga aggcattacg
    15181 ctgcgcttta ttttacaact ctgtctggcg ggcctggcgc tgtttattct catccagctc
    15241 gcaaaactac tctacgcccg acacaaacat gtggatctgt ctgcttcgcg ccgcagccca
    15301 cttactcacc caaaaaatga aggatagaac gatgtcgcaa cattattcag tgtcatggaa
    15361 aaaaggactg gctgcactct gcttactggc tgttgccggg cttagcggct gcgatcaaaa
    15421 agagaatgcg gcggcaaaag tggaatacga cggactttcg aacagccaac cgctgcgcgt
    15481 cgatgccaat aaccatacgg taaccatgct ggtgcaaatt aatggtcgtt tcctcaccga
    15541 cgacactcgt cacggtattg tgtttaaaga tggctccaac ggacataaat cgctgtttat
    15601 ggcttatgcg cccccgaaag cattttatga agccctgaaa gaggcaggtg gtacgccggg
    15661 cgaaaacatg acgatggata ataaagaaac gactcatgtc acaggcagca aactggatat
    15721 ttcggtcaac tggcaagggg cggcaaaagc gtattccttc gatgaagtga ttgttgacag
    15781 taatggcaag aaactggaca tgcgctttgg cggtaattta acggcagcag aagagaagaa
    15841 aacgggttgc ctggtgtgtc tggatagctg cccggtcggc atcgtcagca atgcaacata
    15901 cacttatggt gcggtggaaa aacacggcga agtgaaattc aaaggcaatg cctcagttct
    15961 cccggcggat aacacgctgg caacggttac ctttaaaatc accgaataaa gccaagataa
    16021 aggatgatga agatacaatc gcgtaaaatc tggtactacc gtataaccct catcatcctg
    16081 ttgttcgcta tgctgctcgc atgggcgctg cttcccggcg tccatgagtt tatcaatcgc
    16141 agcgttgcgg cgtttgccgc cgtggaccaa cagggtatag aacgctttat tcagtcttac
    16201 ggcgcactgg cggcagttgt ctcgttcttg ttgatgattt tacaggccat tgctgcaccg
    16261 ctacctgcgt ttttgatcac ctttgccaat gcgtcgctgt ttggcgcgtt ctggggcggc
    16321 ttgctgtcgt ggaccagttc gatggccggc gcggcgctgt gcttttttat cgccagagtg
    16381 atgggccgcg aagtggtgga aaaattaacc ggcaaaaccg tgcttgacag tatggacggc
    16441 tttttcactc gctacggcaa acacaccatc ctggtctgtc ggttattgcc ttttgtccct
    16501 ttcgatccaa tcagctatgc tgccggtctg acttcaatac gttttcgctc gttttttatc
    16561 gccaccgggc ttggtcaatt accggcgact attgtttatt cctgggcagg cagcatgtta
    16621 acaggcggta ctttctggtt tgtcaccgaa ctgtttattc tgtttgccct gaccgtggtg
    16681 atttttatgg cgaagaaaat atggcttgag cgccagaaga ggaatgactg atgggtttac
    16741 cgccgcttag caaaattcct ttcattttac gtccgcaggc gtggctgcat cgtcgccatt
    16801 acggcgaggt gctaagcccg attcgctggt gggggcggat cccgtttatc ttttatttgg
    16861 tgtcaatgtt tgttgggtgg ctggagcgca aacgctcacc gctcgatccg gtagtgcgat
    16921 cgcttgtcag cgcgcgcatt gcgcaaatgt gcctgtgtga gttttgcgtg gatatcacca
    16981 gtatgaaagt cgccgagcgt accggcagca ccgataaact gctggcagtg gctgactggc
    17041 ggcaaagccc gctctttagc gatgaagaac ggctggcgct ggagtacgcc gaagccgcaa
    17101 gcgtcacacc gccaacagtc gatgatgccc tgcgtacccg actggctgcg cattttgacg
    17161 ctcaggcgct caccgaactg acggcattga tcggcctgca aaacctgtcg gctcgtttta
    17221 attctgccat ggacattccc gctcaggggc tgtgccgtat tccggaaaaa cgttcttaag
    17281 gagggaagat gcgccattgt gggtggttgc tgggattgtt atcgctgttt tctttggcaa
    17341 cacatgccag tgactggcag gaaattaaaa atgaggccaa agggcaaacc gtctggttta
    17401 acgcctgggg cggcgatacc gcaattaacc gctatctcga ctgggttagc ggcgagatga
    17461 aaacccatta cgctataaac ctgaagattg ttcgtctcgc ggatgccgca gacgcggtga
    17521 agcgcattca gaccgaagct gctgccggtc gtaaaacggg cggctcggtg gatctgctct
    17581 gggtgaacgg cgaaaac
//
LOCUS       DAJTRN010000082        17255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRN010000082 DAJTRN010000000
VERSION     DAJTRN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OO328_002896"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OO328_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5931387.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO328_002897"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO328_002897"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5931388.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO328_002898"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO328_002898"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5931389.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OO328_002899"
     CDS             complement(2205..2348)
                     /locus_tag="OO328_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5931390.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OO328_002900"
     CDS             complement(3019..3306)
                     /locus_tag="OO328_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5931391.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO328_002901"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO328_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5931392.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OO328_002902"
     CDS             3683..3922
                     /locus_tag="OO328_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931393.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO328_002903"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO328_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5931394.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OO328_002904"
     CDS             5033..6406
                     /locus_tag="OO328_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5931395.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAIRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO328_002905"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO328_002905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5931396.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO328_002906"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO328_002906"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5931397.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO328_002907"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO328_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5931398.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO328_002908"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO328_002908"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5931399.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OO328_002909"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OO328_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5931400.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OO328_002910"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OO328_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OO328_002911"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OO328_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OO328_002912"
     CDS             16524..16760
                     /locus_tag="OO328_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5931401.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..17056
                     /locus_tag="OO328_002913"
     CDS             16865..17056
                     /locus_tag="OO328_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5931402.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17255)
                     /locus_tag="OO328_002914"
     CDS             complement(17081..>17255)
                     /locus_tag="OO328_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5931403.1"
                     /translation="KDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIG
                     NLHYIDTGAVFGS"
BASE COUNT         4186 a         4350 c         4159 g         4560 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatacgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagctccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttt
//
LOCUS       DAJTRN010000083        17116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRN010000083 DAJTRN010000000
VERSION     DAJTRN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO328_002915"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO328_002915"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5931404.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKPPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO328_002916"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO328_002916"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5931405.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO328_002917"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO328_002917"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5931406.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO328_002918"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO328_002918"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5931407.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO328_002919"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO328_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO328_002920"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO328_002920"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5931408.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO328_002921"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO328_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO328_002922"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO328_002922"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5931409.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO328_002923"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO328_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5931410.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="OO328_002924"
     CDS             complement(8973..10211)
                     /locus_tag="OO328_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5931411.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="OO328_002925"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="OO328_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5931412.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO328_002926"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO328_002926"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5931413.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO328_002927"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO328_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5931414.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="OO328_002928"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="OO328_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5931415.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="OO328_002929"
     CDS             14682..15584
                     /locus_tag="OO328_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5931416.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15934
                     /locus_tag="OO328_002930"
                     /pseudo
     CDS             15731..15934
                     /locus_tag="OO328_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(15953..17100)
                     /locus_tag="OO328_002931"
     CDS             complement(join(15953..16863,16863..17100))
                     /locus_tag="OO328_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5931417.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         4225 a         4437 c         4134 g         4320 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagtaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agccccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc gcaatgaatc gccacggata atctagacac
    15961 ttccgagccg ttgataatac tggttttcat attctgtcgg tgacatctgt tcgctagaac
    16021 catgccgacg cttactgtta taaaacattt cgatgtaatc aaaaatatca ctgcgggctt
    16081 cttcccgcgt tccgtagatc tttttcttta tccgttcacg tttcaacaac tggaaaaaac
    16141 tttctgcaac cgcattatca tggcagttac cgcgacggct catgctaccc tccaggccgt
    16201 gtgatttcag gaacgactgc cactcatggc ttgtgtactg actgccctga tccgaatgaa
    16261 ccagcacctg tttttcggga ttacgccgcc atacagccat cagcagtgcg ttcaggacaa
    16321 tgtcctttgt catccgggat tgcatggacc agccgataat tttgcgtgag aacagatcaa
    16381 caacaacggc aagatacagc cagccttcgt gggtcctgat gtaggttatg tccgttaccc
    16441 aacgctcatc aggagcatcc ggattgaact gtcgctggag cctgttgggt gacacgatac
    16501 tggcctcgcc tttacgtgcc cgcgggcttc ggtatccgac ctgagccttt attccgacac
    16561 gtttcatcag tctccagact ctgtttactc cgcactgttg cccgctgtca cgcagatcca
    16621 gatggatttt gcgataacca tagacgcatc ccgattccag ccagaactgt ttaatctgtc
    16681 ctgtcagtct caggtctgcc tgatggcgtt gtgaatgcgg ctgctgaagc caggcgtaaa
    16741 aaccactggg atgaacatcc agcacccgac agagcaggcg aacaggccag caacaggagt
    16801 tgtcacggat aaaggcgtac ctcagtcgga cagctttgcg aagtacgccg cggctttttt
    16861 taatatgtcc cgttcgtcgg taacccgttt cagctctttc tggagacggc ggatctcggc
    16921 ctgagcatct gactgttctt tattagtgga agaatccgga ccgtacttct ttatccaggc
    16981 ataaaggctg tgggtggtga tatcgagacg tgttgcaacg ctggcaacag aataaccgcg
    17041 atcaacaacc tgtttgactg cttcagtttt aaactcttcg ggataacgct taccgctcat
    17101 gggcacctct ctttaa
//
LOCUS       DAJTRN010000084        16794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRN010000084 DAJTRN010000000
VERSION     DAJTRN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            193..1326
                     /gene="fepE"
                     /locus_tag="OO328_002932"
                     /pseudo
     CDS             193..1326
                     /gene="fepE"
                     /locus_tag="OO328_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
     gene            complement(1323..2138)
                     /gene="fepC"
                     /locus_tag="OO328_002933"
     CDS             complement(1323..2138)
                     /gene="fepC"
                     /locus_tag="OO328_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5931418.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(2135..3127)
                     /gene="fepG"
                     /locus_tag="OO328_002934"
     CDS             complement(2135..3127)
                     /gene="fepG"
                     /locus_tag="OO328_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5931419.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3124..4128)
                     /gene="fepD"
                     /locus_tag="OO328_002935"
     CDS             complement(3124..4128)
                     /gene="fepD"
                     /locus_tag="OO328_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5931420.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4239..5471
                     /gene="entS"
                     /locus_tag="OO328_002936"
     CDS             4239..5471
                     /gene="entS"
                     /locus_tag="OO328_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5931421.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGTAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5475..6431)
                     /gene="fepB"
                     /locus_tag="OO328_002937"
     CDS             complement(5475..6431)
                     /gene="fepB"
                     /locus_tag="OO328_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5931422.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6723..7898
                     /gene="entC"
                     /locus_tag="OO328_002938"
                     /pseudo
     CDS             6723..7898
                     /gene="entC"
                     /locus_tag="OO328_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7908..9518
                     /gene="entE"
                     /locus_tag="OO328_002939"
     CDS             7908..9518
                     /gene="entE"
                     /locus_tag="OO328_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5931423.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9532..10389
                     /gene="entB"
                     /locus_tag="OO328_002940"
     CDS             9532..10389
                     /gene="entB"
                     /locus_tag="OO328_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5931424.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10389..11135
                     /gene="entA"
                     /locus_tag="OO328_002941"
     CDS             10389..11135
                     /gene="entA"
                     /locus_tag="OO328_002941"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5931425.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11138..11551
                     /gene="entH"
                     /locus_tag="OO328_002942"
     CDS             11138..11551
                     /gene="entH"
                     /locus_tag="OO328_002942"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5931426.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11732..13837
                     /gene="cstA"
                     /locus_tag="OO328_002943"
     CDS             11732..13837
                     /gene="cstA"
                     /locus_tag="OO328_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5931427.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            14019..14216
                     /locus_tag="OO328_002944"
     CDS             14019..14216
                     /locus_tag="OO328_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5931428.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14226..15314)
                     /gene="hcxA"
                     /locus_tag="OO328_002945"
     CDS             complement(14226..15314)
                     /gene="hcxA"
                     /locus_tag="OO328_002945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5931429.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15423..16585
                     /gene="ybdL"
                     /locus_tag="OO328_002946"
                     /pseudo
     CDS             15423..16585
                     /gene="ybdL"
                     /locus_tag="OO328_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            complement(16586..>16794)
                     /locus_tag="OO328_002947"
     CDS             complement(16586..>16794)
                     /locus_tag="OO328_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5931430.1"
                     /translation="IRHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVL
                     RLKQINGLQELFADRQYSRAWTVK"
BASE COUNT         3721 a         4381 c         4795 g         3897 t
ORIGIN      
        1 aattacagaa aataattatg ggttttttat ttgtttgatt tataggtttg atgaatattt
       61 ctcttaaata gagtgaatgt tgcattcagt ggaaggaatt tacaaacttc agcttcggat
      121 aattctcacc aactggtaaa gtgagcgccg tggagatttt cccctattgg actcattttc
      181 aggttatacc ccatgtcatc attgaatatt aaacagggaa gtgacgctca tttttctgag
      241 tatcctctgg cgtcgcccag taataatgaa attgatttac ttaatctaat cgaagtttta
      301 tggcgggcca aaaaaacggt catggcggtc gtttttgcat ttgcctgcgc aggtttactg
      361 atctctttca tcctgccgca aaaatggacc agctcagccg ttattactcc cgccgaagcc
      421 attcagtggc aggacctgga gaaaaccttt accaaactcc gcgttcttga tctcgatgtt
      481 aatatcgacc gtggcggtgc atttaacctg tttatcaaga agtttcagtc tgttagcttg
      541 ctggaagagt acctgcgttc atcaccttat gtgatggact aattaaaaga ggcgaaaatc
      601 gatgaactgg atttgcatcg cgctattgtt gcattgagcg aaaaaatgaa agcggttgat
      661 gacaatgcta gtaagaaaaa agatgaaccg tcactgtata catcctggac gctaagtttt
      721 accgcgccaa ccagtaaaga ggcgcagacc gttttgagcg ggtatatcga ttatatctct
      781 gcgttggtgg tgaaagagtc gatagaaaac gtccgtaata aactggagat caaaacccag
      841 tttgaaaaag aaaaactggc tcaggatcgc attaaaacga aaaatcaact tgatgcaaac
      901 attcagcgcc tcaattattc actcgacatt gccaacgcgg caggaattaa aaagcccgta
      961 tacagtaatg gtcaggccgt taaagatgac cccgatttct ctatttccct cggcgcagac
     1021 ggtattgaac gcaaactgga aattgaaaaa gcggttactg acgttgcgga actgaacggt
     1081 gaattacgta atcggcagta tcttgtcgag caattaacaa aagcaaatgt caacgatgtg
     1141 aattttacgc cgtttaaata tcagttaagc ccgtcattgc cagtgaaaaa agacggtccg
     1201 ggtaaggcga ttattgtgat cctttccgcg ctgatcggcg ggatggtggc ttgtggtagc
     1261 gtgttattgc gctatgcgat ggcatccaga aaacaggatg ccatgatggc agaccactta
     1321 gtttaactat ttgcggttga aggtgccgtt cgtccgagcg gcaccacaag cggcattccg
     1381 gctaccggat cgtcaatgat catgcatcgc agaccataaa tgcgctcaat cagttcagca
     1441 gtgacaatct ccttcggcgc tccctgagca acaattttcc cttcccgcaa tgcaatcaaa
     1501 tggctggcgt aacgacaggc ctgattaaga tcgtgcagca ccgccgccag ggtatagcct
     1561 ttctcgcggt tcagttcgct taacagttcc agtagatcaa tctgatgact gatatccagc
     1621 caggtcgtgg gttcgtcgag cagcataatt gccgtttcct gggccagcac catcgcgatc
     1681 cacgctcgct ggcgttgtcc gccagaaagg gtatccacgc tttgatctgc cagatgagtt
     1741 attcccgtgg cctgcatcgc tttcgttacc gcttcttcat cctctttgcg ccagcgggta
     1801 aacagcggtt gatgcggata acgtccacgc gccaccagct cctgcacggt gatatcgccc
     1861 ggcgtggtag cattttgcgc caacagacca atccggcgtg caacttcttt actggcgtaa
     1921 tgttgaatgt gctcgccatc cagccagaca tgcccgtgag caggcgtcat caggcggctt
     1981 aaggtacgca gtaacgtgga tttaccacag ccatttggtc cgataattgc cgtgaagtga
     2041 ccatcaggaa tttctacagt cagattttcc gcaacggtat atttgccata tcccagggtt
     2101 aactgttcgc cgcgcaaacg ggctactgat tcggtcattt tttgcgagac tcctgaatta
     2161 acaagacgat aaggtaaata ccgccgaggc tgacggtaac gacgccaacc ggaagttgat
     2221 acggcataaa cagttgttgg gcgcagagaa cggccgccag cagtaacagc gctccgcaca
     2281 gcgccgcctg ggtcagcccc cagcgagcgg tgccgctaat gcgtcgggca atgtgcggcg
     2341 cgactaaagc aataaaggaa atcggcccgg caagcgccgt tgccgcagcg gtaagcacca
     2401 ctgcaaccag catcattaac agacgcgaac gctcgacgct gacgcccagc gcgcaggcgg
     2461 tatcatcgcc catctccagc aagcgcattc gccgcaccag caaggtggcg gcaatgagca
     2521 tcaatataat gatgggtgcg gaaggcgagg tttttgccca cgtcaggccg ttgagcgatc
     2581 cggcattcca caaacctgct gttagcgccg tttctaaaga cgctttcagc aacagccagg
     2641 tattaaaggc caccagcatg gcgcgaacgc cgataccgat aataatcaac cgaaaggtgt
     2701 cgataccgtt acgccaggcg agcagccaga ccaacagcga agtgataatg ccgcccacca
     2761 ttgctgccag cgcgatagcc gtcagatcct gaccaaacag caccatcgcc accagcacgc
     2821 cgctccacgc cccggtgtta aagcccatta cgtcagggct gccgagcggg ttacgcatca
     2881 gcgactgaaa aatcgcgccg ctgacgccca gcgccgcgcc aatcaacagc gccatcagca
     2941 cgcgtggtaa acgccattcg gtgaccacca tcgtcatact gcgcggcgca tcgcccatca
     3001 acgcggcgaa tacctgcgag atttccagcg tgacggcacc gctgcgtaat ccccagatac
     3061 ctgcaaccac acaggcggaa atcagcagca aacaggtgat gagtaatcgg cgagagacgt
     3121 aaatcatgca ccacctcgcg ttttacgtcg cacgaggaag atcagcaccg gtgcaccaat
     3181 aaacgcactg accacagaaa cgcgcagttc gccgggcaca atcacccgcc cgatgatatc
     3241 ggcaaacagc agcagggcag gggtagcaag tagcgtgacg ggcagcgacc agcgatgatc
     3301 ggcacctacc agccagcgcg ccatgtgcgg catcatcagg ccgataaagg caatcgggcc
     3361 aactaccgcc gtcgcactac cacaaagcac ggtaatcgcc agcagaccaa tcaactgtgt
     3421 gcgcgccacg cgactgccca gcgccgtcgc ggtgtcactg ccgaggctca aactgttcag
     3481 cgcgcgactc agtaatagcg cagttgctcc ggcgatcaga accgggatca gcaccacttt
     3541 taaggtatgt agattgcgaa tatccagcga accggcttgc cagaaacgca gttgatcgta
     3601 gacgtcagga ttaagcaggg cgatgccgct ggtcagtcct tccagcaccg ccgccagcgc
     3661 cacgcccgcc agggttaaac gcaccggact taactgccct ccaccctgac tgccggtaaa
     3721 ggcaacaatc aatgaggcca ccagcgcccc ggcgaaggcc atcgccagtt gttcctgtgc
     3781 ggatgagtaa ccaaacagcg ccgcacccag cacaatggca aagctggctc cggcgttcac
     3841 gccaagcaag ccggggtcgg caagtgggtt tcgggtgagg gtttgcatta acgccccggc
     3901 aaggccaagc gcgccgcctg ccagtaaacc ggcaagggta cgcggcagcc gcgcgtcgag
     3961 cacgatggtg cagtcggcgc tctggcaggt gccggagaag gcctccagca ctacggaagc
     4021 ggggagtgat tttgccccaa tgagcaggct taatgccgtc gcgataatca gtaataacag
     4081 caatccgggc acggcaatgg cgcgtgtcac ggcaacagaa ccagatatac gaacttccat
     4141 gataatgaaa ttaattatcg ttatcgatct tatttggata tgttaacatg tgcagcctaa
     4201 gaataggtat ttaaaatatt tgatggcaag gcattgtaat gaataaacaa tcctggctgc
     4261 ttaacctcag cctgttgaaa acgcacccgg cgtttcgcgc agtattcctc gctcgtttta
     4321 tctcaattgt gtctctgggt ttgctcggcg tcgcggtgcc ggtgcagatc cagatgatga
     4381 cccattctac ctggcaggtg gggctttcgg tgacgctgac cggcggcgcg atgtttgttg
     4441 gcctgatggt tggcggtgtg ctggcggatc gctatgaacg caaaaaagtg attttgctgg
     4501 cgcgcggcac ctgtggcatt ggcttcattg gactgtgcct taatgcactg ctgccggagc
     4561 cgtcattgct ggcaatctat ttacttggtt tatgggatgg ttttttcgca tcacttggtg
     4621 ttacggcgct actggcggca acatctgcac tggtagggcg tgaaaactta atgcaggccg
     4681 gggcgatcac catgttgacc gtgcgcctgg ggtcggtgat ttcgcccatg attggcggtt
     4741 tattgctggc gaccggcggc gtagcctgga actacgggct ggcggcggcg ggcacgttta
     4801 ttaccttgtt accgttatta agccttccgg agttgccacc gccaccgcag ccgcttgagc
     4861 atccgttgaa atcattactg gcaggatttc gttttctgct tgccagcccg ctgctgggtg
     4921 gtttattgac gatggcgagc gcggtgctgg tactgtatcc ggcgctggct gacaactggc
     4981 agatgtcagc ggcacagatt ggttttctct acgcggcgat cccgctcggc acggctattg
     5041 gtgcgttaac cagcgggaag ctggcacata gtgcgcgacc agggttattg atgctgctct
     5101 ccacgctggg atcgttcctc gccattggtc tgtttggcct gatgccgatg tggattttag
     5161 gcgtggtttg tctggcgctg ttcggctggc tgagcgcggt tagttcgttg ctgcaatata
     5221 caatgctgca aacgcaaacc ccggaagcga tgttagggcg gattaacggt ttgtggacgg
     5281 cgcagaacgt gacgggcgat gccataggcg cggcgctgct gggtggtttg ggcgcgatga
     5341 tgacaccggt tgcttccgca agcgcgagcg gttttggttt gttgattatc ggcgtgttgt
     5401 tattgctggt gctggtggag ttgcgacgtt ttcgccagac gccgccgcag gtgacagcgt
     5461 ccgacagtta atgcttaaaa cagcgcctta agccgttcca gcacttgcat ggcgctgtag
     5521 taatccaggc ggaacgtctc ggttcccagc gcataaacct gcttgttttg taccgcaggc
     5581 aggtgcgcga gcagtggatt ggcataaatc gcatcggcat ctttctgatc accagcgaac
     5641 aggaatagcg attcaccgtt taaccccgca gccagatttt ccccaccaag ctgaatgatg
     5701 tcatggcgtt tgccctggct ttggctggcg tttaaccctg cgggcaattt cgccagcgta
     5761 aagccgagtt gttccagcat ctgcccttgt gctgattccg gcgtccagag attggcactg
     5821 tgtgcagcgg cggtatagac aatggcagtg accggctgcg gcggtaattt gatttgctct
     5881 ttcgccgctg ccagttgctt atcaaactgt gcaatccgct ctgccgcttg tttctcatgc
     5941 ccggtaattt cgccaagctg cgttaacagc gactgccagc ttttgtcgtc gtaattgatg
     6001 attaatgtcg gggcgatggt ggaaagctga tcatacagcg ccagcgccga atccccgccg
     6061 gttgcgctaa ttaaaatcag atccggcatt tgcgcggcaa cggcttcggc gctcggttcg
     6121 ccgatataga gccgttgcag tttgcgttct ttcgccacct tgctccactg gcgtaaaaag
     6181 ccctggtcat ctgcgacgcg gttattcggc gtggtcgcgc cgctggcgat caccggagca
     6241 tcaatcgcca gcagtgatcc ggtcagggtg acgctggtgg aaacaatacg ctgcggctgg
     6301 ctttccaggg tatgagtgcc ccggctgtca gtaatctgac gcggccagtc ggcggcctga
     6361 actacggcta ttcctgaaag caaaagtcct gttaatagaa gggcgttgcg gtagagcggg
     6421 gcgagtctca caagtcagct tcctgttatt aataaggtta agggcgtaat gacaaattcg
     6481 acaaagcgca caatccgtcc ctttggggag agggggcaaa acggcgcagg acatcacatt
     6541 gcgcttatgc gaattcatca ataatgcttc tcattttcat tgtaaccaca accagatgca
     6601 accccgagtt gcagattgcg ttacctcaag agttgacata gtgcgcgttt gcttttaggt
     6661 tagcggccga aaatataagt gataatcatt attaaagcct ttatcatttt gtggaggatg
     6721 atatggatac gtcactggct gaggaagtac agcagaccat ggcaacactt gcgcccaatc
     6781 gctttttctt tatgtcgccg taccgcagtt ttacgacgtc aggatgtttc gcccgtttcg
     6841 atgaaccggc tgtgaacggg gattcgcccg acagtccctt ccagcaaaaa ctcgccgcgc
     6901 tgtttgccga tgccaaagcg cagggcatca aaaatccggt gatggtcggg gcgattccct
     6961 tcgatccacg tcagccttcg tcgctgtata ttcccgaatc ctagcagtcg ttctcccgtc
     7021 aggaaaaaca gacctcagcc cgctgtttta cccgcagcca gtcgctgaac gtggtggaac
     7081 gctaggcaat tcctgaacaa accacgtttg aacagatggg tgcccgcgcc gccgcactta
     7141 ccgccacgcc gcaggtcgac aaagtggtgt tgtcacgatt gattgatatc accactgacg
     7201 ccgccattga tagtggcgta ttgctggaac ggttgattgc gcaaaacccg gttagttaca
     7261 acttccatgt cccgctggct gatggtggcg tcctgctggg ggccagcccg gaactgctgc
     7321 tacgtaaaga cggcgagcgt tttagctcca ttccgttagc cggttccgcg cgtcgtcagc
     7381 cggatgaagt gctcgatcgc gaagcgggta atcgtctgct ggcgtcagaa aaagatcgcc
     7441 atgaacatga actggggact caggcgatga aagaggtact gcgcgaacgc agtagtgagt
     7501 tacacgttcc ctcctctcca caattgatta ccacgccgac gctgtggcat ctcgcaactc
     7561 cctttgaagg taaagcgaat tcgcaagaaa acgcactgac tctggcctgt ctgctgcatc
     7621 cgacccccgc gctgagcggt ttcccgcatc aggccgcgac ccaggttatt gctgaactgg
     7681 agccgttcga ccgcgaactg tttggcggca ttgtgggttg gtgtgacagc gaaggtaacg
     7741 gcgaatgggt ggtgaccatc cgctgcgcga agctgcggga aaatcaggtg cgtctgtttg
     7801 ccggagcggg gattgtgcct gcgtcgtcac cgttgggtga gtggcgcgaa acaggcgtca
     7861 aactttctac catgttgaac gtttttggat tgcattaagg agcgaggatg agcattccat
     7921 tcacccgctg gccggaagag tttgcccgtc gctatcggga aaaaggctac tggcaggatt
     7981 tgccactgac cgacattctg actcgccacg ctgcgagtga cagcatcgcg gttatcgacg
     8041 gcgagcggca gttgagttat cgggagctga atcaggcggc ggataacctc gcctgtagtt
     8101 tgcgccgtca gggcattaaa cctggtgaaa ccgcgctggt acaactgggt aacgtcgctg
     8161 aactttacat tacctttttc gcgctgctga aactgggcgt cgcgccggtg ctggcgctgt
     8221 tcagccatca gcgtagtgaa ctgaacgcct acgccagcca gattgaaccg gcgttactga
     8281 ttgccgatcg ccagcatgcg ctatttagcg gggatgattt cctcaacaca tttgttgcag
     8341 agcattcttc cattcgcgtg gtgcaactgc acaacgacag cggtgagcat aacttgcagg
     8401 atgcgattaa ccatccggca gagggtttta ctgccacgcc atcacctgct gatgaagtgg
     8461 cctatttcca gctttccggc ggcaccaccg gcacaccgaa actgatcccg cgcactcata
     8521 acgactatta ctacagcgtg cgtcgtagcg ccgagatttg tcagttcaca caacagacgc
     8581 gctacctgtg ctcgatcccg gcggctcata actacactat gagttcgcca ggatcgctgg
     8641 gcgtctttct tgccggagga acggttgttc tggcggccga tcccagcgcc acgctttgct
     8701 tcccgttgat tgcaaaacat caggttaacg ttaccgcgct ggtgccgccg gcagtcagcc
     8761 tgtggttgca ggcgctggct gaaggcgaaa gccgggcgca gcttgcctcg ctgaaactgt
     8821 tacaggtcgg cggcgcacgt ctttctgcca cgcttgcagc gcgtattccc gctgagattg
     8881 gctgccagtt gcagcaggtg tttggcatgg cggaagggct ggtgaactac acccgtcttg
     8941 atgatagcgc ggagaaaatt atccataccc agggttaccc aatgtgtccg gacgatgaag
     9001 tgtgggttgc cgatgccgaa ggaaatccac tgccgcaagg ggaagttgga cgcctgatga
     9061 cgcgcgggcc gtacaccttc cgtggctatt acaaaagtcc gcagcacaat gccagtgcct
     9121 ttgatgccaa cggtttttac tgttccggcg atctgatctc tattgatcca gagggttaca
     9181 tcaccgtgca ggggcgcgag aaagatcaga tcaaccgtgg cggcgagaag atcgctgccg
     9241 aagatatcga aaacctgctg ctgagccatc cggcagtgat ctatgccgca ctggtgagca
     9301 tggaaaatga gctgatgggc gaaaaaagct gtgcttatct ggtggtaaaa gagccgctgc
     9361 gcgcggtgca ggtgcgtcgt ttcctgcgtg aacagggtat tgccgaattt aaattaccgg
     9421 atcgcgtgga gtgtgtggat tcacttccgc tgacggcggt cgggaaagtc gataaaaaac
     9481 aattacgtca gtggctggcg tcacgcgcat cagcctgaag gagagaacac gatggctatt
     9541 ccaaaattac aggcttacgc actgccggag tctcacgata ttccgcagaa taaagttgac
     9601 tgggcctttg aaccgcaacg tgccgcgttg ttaatccatg atatgcagga ctattttgtc
     9661 agcttctggg gcgagaactg cccgatgatg gagcaggtga tcgcgaatat tgctgcgctg
     9721 cgcgactact gcaaacagca caatatcccg gtttattaca ccgcccagcc gaaagagcag
     9781 agcgatgaag atcgggcgct gttgaatgat atgtgggggc cgggcctgac ccgctcgccg
     9841 gaacagcaaa aggtggtgga tcgcctgacg ccagatgccg acgacacggt gctggtgaag
     9901 tggcgctaca gcgcgtttca tcgttctccg ctggagcaaa tgctcaaaga gagtggacgt
     9961 aaccagctga ttattacagg agtatatgcc cacattggct gtatgaccac cgcaaccgac
    10021 gcatttatgc gcgatattaa accgtttatg gtggcggatg cgctggctga tttcagccgt
    10081 gacgagcatt tgatgtcgct gaaatatgtg gccggacgtt ctggccgggt ggtgatgacc
    10141 gaagaattac tgccagcacc tatccccgcc agcaaagcgg cgctgcgtga ggtgatcctg
    10201 ccgttgctgg acgagtccga tgaaccgttc gatgacgaca acctgatcga ctacggtctg
    10261 gattcggtgc gcatgatggc gctggcggcg cgctggcgca aagtgcatgg tgatatcgac
    10321 tttgtcatgc tggcgaaaaa cccgaccatc gacgcctggt ggaagctact ctcccgcgag
    10381 gtgaaataat ggatttcagc ggtaaaaatg tctgggtaac cggcgcaggt aaaggtatcg
    10441 gctacgccac ggcgctgacg tttgttgagg cgggagcgaa agttacaggt tttgatcaag
    10501 cgttcactca ggagcaatat ccctttgcga ccgaagtaat ggatgttgcc gacgccgggc
    10561 aggtggcgca ggtatgtcag cgactgttag ctgaaacgga gcgactggac gtgctgatca
    10621 atgcggcggg aattttgcgc atgggcgcga ccgatcagct cagtaaagag gactggcagc
    10681 agacttttgc ggttaacgtc ggcggtgcgt ttaacctgtt ccagcaaacc atgaaccagt
    10741 ttcgccgtca gcggggcggg gcgattgtca ctgtggcgtc cgacgccgca cacacggcgc
    10801 gtattggtat gagtgcttat ggcgcatcga aagcggcgct gaaaagcctg gcgttgagcg
    10861 tcgggctgga actggcgggt agcggcgtgc gctgtaatgt ggtttcgcct ggctccaccg
    10921 acaccgatat gcaacgcacg ctgtgggtga gcgatgacgc cgaagaacag cgtattcgcg
    10981 gctttggcga gcagtttaaa ctcggcattc cgctggggaa aatcgcccgt ccacaagaga
    11041 tcgccaacac gattttgttc ctcgcctctg acctcgccag ccatatcacc ctacaggata
    11101 ttgtggtcga tggcggctca acgctggggg cataagcatg atctggaaac gccatttaac
    11161 gctcgacgaa ctgaacgcca ccagcgataa cacaatggtg gcgcatctgg gaattgtgta
    11221 tacccttctg ggagatgatg tgctggaagc cgaaatgccg gttgataccc gtactcatca
    11281 gccgtttggc ctgctgcatg gcggcgcgtc ggcggcgctg gctgaaacgc tgggatcgat
    11341 ggccggattt atgatgaccc gtgacgggca gtgtgtggtg ggcacggaac ttaacgccac
    11401 ccatcatcgc ccggtgtctg aaggcaaggt acgcggcgtc tgccagccgc tgcatcttgg
    11461 gaggcaaaat cagagttggg aaatcgtcgt tttcgatgaa caggggcggc gttgctgcac
    11521 ttgtcggctg ggtacggcag ttttgggatg agcccgacag aattaaatga gattgcagga
    11581 aaactcggtt aacggagtga tcgagttaac attgttaagt taaatattgg tttcaactcc
    11641 gatttacatg gttaccgtgt tgttaaatcg tacaaagatg ttatagaaac aaaatgtaac
    11701 atctctatgg acacgcacac ggataacaac tatgaacaaa tcagggaaat acctcgtctg
    11761 gacagtgctc tctgtaatgg gagcatttgc tctgggatac attgctttaa atcgtgggga
    11821 acagatcaac gcgctgtgga ttgtggtggc gtcggtctgt atctatctga tcgcttaccg
    11881 tttttatggg ctgtatatcg ccaaaaatgt gctggcggtt gacccgacgc gtatgacgcc
    11941 agcggtgcgc cataacgacg ggctggacta tgtgccgacg gacaagaaag tgctgttcgg
    12001 tcaccatttt gcggccattg ccggagcagg gccgctggtg gggccggtgc tggcagcgca
    12061 aatgggctac ctgccgggga tgatctggct gctcgccggg gtggttctcg ccggtgcggt
    12121 gcaggatttc atggtgctgt ttgtttctac gcgccgtgac ggtcgctcgc tgggtgagct
    12181 ggtcaaagaa gagatggggc caactgccgg ggtgattgcg ctggtagcct gctttatgat
    12241 catggtcatt atccttgcag tactggcgat gatcgtggtg aaagccctga ctcatagccc
    12301 gtggggaacg tataccgttg cgttcaccat tccgctggcg atttttatgg gaatctacct
    12361 gcgctatctg cgtccgggcc gcattggcga agtgtcagtg attggcctgg tgttcctgat
    12421 tttcgccatt atctctggcg gctgggtggc agaaagtccg acctgggcac cgtactttga
    12481 ctttaccggc gtgcagctga cctggatgct ggtgggctac ggttttgtgg cggcggtgct
    12541 gccggtgtgg ttgctgctgg ccccgcgtga ctatctctcg accttcctga aaatcgggac
    12601 tatcgtcggt ctggcggtag gcattttgat tatgcgcccg acgctgacca tgcctgcgct
    12661 gaccaaattt gtcgatggca ctggcccggt atggaccggt aacctgttcc cgttcctgtt
    12721 tatcaccatc gcctgtggtg cggtgtctgg cttccatgcg ctgatctctt ccggcaccac
    12781 gccgaagatg ctggcgaacg aagggcaggc gtgctttatc ggctacggtg ggatgttgat
    12841 ggaatccttc gtggcgatta tggcgctagt ttctgcctgt atcatcgatc cgggcgtgta
    12901 ttttgccatg aacagcccaa tggcggtgct ggctccggca gggacggcgg atgtagtcgc
    12961 ttctgccgcg caggtggtga gtagctgggg ctttagcatt acaccagata cgctcaacca
    13021 gattgccagc gaagtgggtg aacagtcgat catttcccgt gcgggcggtg cgccgacgtt
    13081 ggcggtgggg atggcttaca tcctgcacgg tgcgctgggt ggtatgatgg atgtggcgtt
    13141 ctggtatcac ttcgccattc tgtttgaagc tctgtttatt ctgacggcgg tagatgcggg
    13201 tacgcgtgct gcgcgcttta tgttgcagga tctgctgggc gtggtgtctc ctggcctgaa
    13261 gcggaccgat tcactgcctg ctaacctgct ggcaacagcg ctgtgcgtac tggcgtgggg
    13321 ctacttcctc catcagagcg tggtcgatcc gctgggcggt attaacactc tgtggccgct
    13381 gtttggtatt gccaaccaga tgctggcagg gatggcgctg atgctctgtg ccgtggtgtt
    13441 gttcaagatg aaacgtcaac gttacgcctg ggtggcgctg gtaccaacgg cctggctgct
    13501 gatttgtacc ctgaccgctg actggctgaa agcgtttagc ccggatgcga aagtcggctt
    13561 cctggtcatt gctaacaagt tccaggcaat gatcgacagc ggcaatattc cgtcgcagta
    13621 tactgagtca cagctggcgc aactggtgtt caacaaccgt ctggatgccg ggttaaccat
    13681 cttctttatg gtggtcgtgg tggtcctggc actgttctcg attaagacgg cacttgcggc
    13741 attgaaagat ccgaagccaa cggcgaaaga aacgccgtat gagccgatgc cggaaaatgt
    13801 cgaggagatc gtagcgcagg cgaaaggcgc gcactaaagt cagagtgagg ggggatgttg
    13861 gcgaatgttg gcatagtgcc gagggttccc cctcacccta accctctccc cgatggggcg
    13921 aggggactga ccgagtgcgt tgatagcatt tgtaggccgg ataaagcgtt cacgccgcat
    13981 ccggcactct ttcagcaaca tggttagcgg aggccaagat gtttgattca ctggcaaaag
    14041 ccggaaaata tttaggtcag gcggcgaagc tgatgattgg tatgcctgat tacgacaact
    14101 atgtcgaaca tatgcgggtt aatcatcccg atcaaacgcc gatgacctac gaggagtttt
    14161 tccgtgagcg gcaggacgcg cgctacggtg gaaaaggcgg cgcgcgctgc tgctaaattt
    14221 gcacgtcagg ctttaaacga ttccactttt tcgaacgctg cacgcaacgt atctggtgtc
    14281 agcgtgactg gcaggtaatg aatggactcc accgggcgca aggtgtgggc aatcactttg
    14341 tcgatctccg cctgattatt gatatccacc tccagctccg ccagcgtagt cggcagatga
    14401 aaacgctgat acgccccggt taattgcgcc agcacatcat cctgacccag caaggcgctt
    14461 tgcaccagaa ttccgtaagc gactttggtg ccgtgaagaa acttctcggt ttgcggcagg
    14521 acagtcagac cgttatgcac ggcatgagcc gccgccacac gcgtaaaacg atcgcccaga
    14581 ccaccgacca tcccaccacc agcaataata gcatccacca catcgcaaaa tgattgcgtt
    14641 aactgctgat tttgctgatc ggcaagtgcc tgttcgctac tgtttaacaa gacgtcgcga
    14701 atggcctgcg cattattgat ccccagccgc acggttagcg gcaacgtttc tggttgcgga
    14761 gccagcacca ccgcttcata ccatttcgcc agcgtgtcac cgatccccgc cagcagatat
    14821 tgttgcggtg cattgaggat aatctccggt tccaccagca ccataaaatt ggcgtcgtcg
    14881 aaaatctcat aatgcagcgc ctgtccggca tcgttatacc agacggagag cggtgtccag
    14941 gctgcgcagg tggcggcgat cgtcggagcg gcaacaaatg gcagaccgag acggcgggcg
    15001 agggctttcg cggtgtcgag cagtgcgccg ccgccgacgc caatcaccac gctgcgatcg
    15061 tcaccggact cagccgccag ttgctgtaca tcgctttcgc tgcaatgacc acgaaacaaa
    15121 atatgctttg cccctggaag tccaaacgct ggcggaagtt tggtttgcgc cgcagcaatg
    15181 gcgcgtttgc cgtagatcca caccgcgcga gaaagttgtt catcagtgaa aaaattgtgc
    15241 aggtgattga aacttcctgg atgtgaaaag tagttagccg ggccgacgac cacgcggata
    15301 ggattgtgag gcatgttgtg ttgtccttat tgttttattt agacatctaa acgtcttgat
    15361 tgccaaatac tggcatcgtg ttatagtgcc ttcaacacgc aacttcgtca ggtacaataa
    15421 aaatgacaaa taaccctctg attccacaaa gcaaacttcc acaacttggc accactattt
    15481 tcacccagat gagcgcgctg gcgcagcaac accaggcgat taacctgtcg caaggctttc
    15541 ctgattttga tggtccgcgc tatttacagg agcggctggc gtaccacgtt gcccaggggg
    15601 caaaccaata cgcgcccatg accggcgtgc aggccttgcg cgaggcgatt gctcagaaaa
    15661 cggaacgttt gtatggctat caaccagatg ccgatagcga tatcaccgta acggcagggg
    15721 cgacggaagc gttatacgcg gcgattaccg cactggtgcg caatggcgat gaagtgattt
    15781 gttttgatcc cagctatgac agttacgccc ccgccatcgc gctttctggg ggaatatagt
    15841 gaaacgtatg gcactgcaac caccgcattt tcgcgttgac tggcaggaat ttgccgcatt
    15901 attaagcgag cgcaccagac tggtgatcct caacactccg cataacccca gtgcaactgt
    15961 ctggcagcag gctgatttcg ccgctttgtg gcaggcgatc gccgggcacg agatttttgt
    16021 cattagcgat gaagtctacg agcacatcaa cttttcacaa cagggccatg ccagtgtgct
    16081 ggcgcatccg cagctgcgtg agcgggcagt ggcggtttct tcatttggca agacctatca
    16141 tatgaccggc tggaaagtgg gttattgtgt tgcgccagcg cccatcagcg ccgaaattcg
    16201 caaggtacat cagtatctga ccttttcggt gaataccccg gcacagctgg cgcttgctga
    16261 tatgctacgt gcagaacctg agcattatct tgcgttaccg gacttttatc gccagaagcg
    16321 cgatattctg gtgaatgctt taaatgaaag ccggctggag attttaccgt gtgaaggtac
    16381 atactttttg ctggtggatt acagcgcggt ttctaccctg gatgatgttg agttttgcca
    16441 gtggctgacg caggagcacg gcgtagcggc gattccgctg tcggtgtttt gcgccgatcc
    16501 cttcccacat aaactgattc gtctctgttt tgccaagaag gaatcgacgt tgctggcagc
    16561 agctgaacgc ctgcgccagc tttagctatt taaccgtcca ggcgcgggaa tattgacggt
    16621 cggcaaacaa ctcttgcagg ccgttaattt gcttcagtcg cagcacttcg tcgctgtcca
    16681 ttcccagctc tttgccaatt ttattatcat cccatcccaa ctggctaagt tcacggacga
    16741 tttccgacat ggcggtgatt tgatggcgtc cacgagcgcg attatgacga atag
//
LOCUS       DAJTRN010000085        16389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRN010000085 DAJTRN010000000
VERSION     DAJTRN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO328_002948"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO328_002948"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5931431.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO328_002949"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO328_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5931432.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO328_002950"
     CDS             2339..2554
                     /locus_tag="OO328_002950"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931433.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO328_002951"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO328_002951"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5931434.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO328_002952"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO328_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5931435.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO328_002953"
     CDS             5920..6417
                     /locus_tag="OO328_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5931436.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO328_002954"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO328_002954"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5931437.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO328_002955"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO328_002955"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5931438.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO328_002956"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO328_002956"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5931439.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO328_002957"
     CDS             9102..9665
                     /locus_tag="OO328_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5931440.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO328_002958"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO328_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5931441.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO328_002959"
     CDS             complement(10257..11237)
                     /locus_tag="OO328_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5931442.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO328_002960"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO328_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5931443.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO328_002961"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO328_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5931444.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO328_002962"
     CDS             12540..12830
                     /locus_tag="OO328_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5931445.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO328_002963"
     CDS             12838..13431
                     /locus_tag="OO328_002963"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5931446.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO328_002964"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO328_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5931447.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="OO328_002965"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="OO328_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="OO328_002966"
     CDS             15882..16385
                     /locus_tag="OO328_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5931448.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4124 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAJTRN010000086        16064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRN010000086 DAJTRN010000000
VERSION     DAJTRN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..2072)
                     /gene="rnr"
                     /locus_tag="OO328_002967"
     CDS             complement(<1..2072)
                     /gene="rnr"
                     /locus_tag="OO328_002967"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5931449.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRL"
     gene            complement(2111..2536)
                     /gene="nsrR"
                     /locus_tag="OO328_002968"
     CDS             complement(2111..2536)
                     /gene="nsrR"
                     /locus_tag="OO328_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5931450.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(2741..4039)
                     /gene="purA"
                     /locus_tag="OO328_002969"
     CDS             complement(2741..4039)
                     /gene="purA"
                     /locus_tag="OO328_002969"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5931451.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4143..4340)
                     /locus_tag="OO328_002970"
     CDS             complement(4143..4340)
                     /locus_tag="OO328_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5931452.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4422..5426)
                     /gene="hflC"
                     /locus_tag="OO328_002971"
     CDS             complement(4422..5426)
                     /gene="hflC"
                     /locus_tag="OO328_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5931453.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5429..6688)
                     /gene="hflK"
                     /locus_tag="OO328_002972"
     CDS             complement(5429..6688)
                     /gene="hflK"
                     /locus_tag="OO328_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5931454.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(6774..8054)
                     /gene="hflX"
                     /locus_tag="OO328_002973"
     CDS             complement(6774..8054)
                     /gene="hflX"
                     /locus_tag="OO328_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5931455.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8130..8438)
                     /gene="hfq"
                     /locus_tag="OO328_002974"
     CDS             complement(8130..8438)
                     /gene="hfq"
                     /locus_tag="OO328_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5931456.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8524..9474)
                     /gene="miaA"
                     /locus_tag="OO328_002975"
     CDS             complement(8524..9474)
                     /gene="miaA"
                     /locus_tag="OO328_002975"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5931457.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9467..11314)
                     /gene="mutL"
                     /locus_tag="OO328_002976"
     CDS             complement(9467..11314)
                     /gene="mutL"
                     /locus_tag="OO328_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5931458.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11324..12661)
                     /gene="amiB"
                     /locus_tag="OO328_002977"
     CDS             complement(11324..12661)
                     /gene="amiB"
                     /locus_tag="OO328_002977"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5931459.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(12680..13141)
                     /gene="tsaE"
                     /locus_tag="OO328_002978"
     CDS             complement(12680..13141)
                     /gene="tsaE"
                     /locus_tag="OO328_002978"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5931460.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13113..14660)
                     /gene="nnr"
                     /locus_tag="OO328_002979"
     CDS             complement(13113..14660)
                     /gene="nnr"
                     /locus_tag="OO328_002979"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5931461.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            14659..15798
                     /gene="queG"
                     /locus_tag="OO328_002980"
     CDS             14659..15798
                     /gene="queG"
                     /locus_tag="OO328_002980"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5931462.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(15781..15834)
                     /locus_tag="OO328_002981"
     CDS             complement(15781..15834)
                     /locus_tag="OO328_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931463.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3684 a         4577 c         4046 g         3757 t
ORIGIN      
        1 aggcgttgcc ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc
       61 agaccatcaa tgaacaagtc gtccagacgg acgaagaagc caaagccagt gacgctggaa
      121 attacgcctt taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca
      181 gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt
      241 tgcaacatct cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc
      301 ccctgctctt tcgccagcag atatttaatg gcgcggtgca gcgtcaggtc aggataacga
      361 cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca gaccgaagtg accacggttt
      421 tctggatcgt aaatcgcctg tttcatcgag cgtagcagca tggtttgcag catttctgca
      481 tcaggacggt cggcaaccga ctccagcagt tccgcgtagt cacgcggttc cggcttgtta
      541 ccacccggca gctccagccc cagctccgcc agcactgaac ggaaagaggt aatcgcttcg
      601 gtgctcggct tgtcgtgaat acggaacagt gccggttctt tcgctttctc aacgaaacgc
      661 gccgccgaga tattcgccag aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc
      721 tgggtctgtt cgatacgttc aatacggcgt tcagcgttga aaatgaactt cgcttcttcg
      781 ctctcaaatg agatcccacc gcgttcttca cgggctttat ccagcacttt atagaggtta
      841 tgcaactctt cgagatgctt aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc
      901 tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg
      961 tagaatttgt agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac
     1021 aggcggtcta cctgcgggtt gagcgaacac aggccgttag agagcacttc cggcagcatc
     1081 gggataacct gcgaagggaa gtacaccgac gtgccacggt tacgcgcttc tctgtccagc
     1141 ggcgttggcg gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg
     1201 ccgccgcgtt ttttctcgca gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca
     1261 atggtgacca gcggtaaatc gcgcaaatcg acacggcccg cttttgcttc ttccggcact
     1321 tcttctttca gccccgcaac ctgttgctca acagcctgcg gccagatata cggaatttca
     1381 tgggtacgca gagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg
     1441 acgattttac ccaccgcttt ggtgcggcga gtcggacgct gagtcagttc gactacgacc
     1501 acaaagccca tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga
     1561 cggctatcgt caggaaccac aaagccgacg cccgcttcgg taaagtagcg accaacaatc
     1621 tggctggttt ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca
     1681 gcacccagcg gctgcgccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg
     1741 gagagataca aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtga
     1801 ccaataacgg tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga
     1861 gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc
     1921 tgctcttcgc cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca
     1981 cgtttggtta aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca
     2041 gcttcgcgtt cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg
     2101 aagattttcg tcactccacc agcaataatt tataaagcgg ttgattctct tcaaccaaat
     2161 cggcaagcgt gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct
     2221 gtttcaaccg acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata
     2281 agggctccag ctcgcgcacc acatcaccaa tacgtatcgc actcgccggt ttacccaggc
     2341 gaatgccgcc attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga
     2401 ttattttgac catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg
     2461 tcatccgccc ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga
     2521 aactcgttaa ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga
     2581 tgataaacca gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg
     2641 aggatttaac actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact
     2701 gttagcccgg caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacgggtcgc
     2761 gcagaatcat ggtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccgg
     2821 tcagctcttc aatacgcttg atgtagttca gcgccgcctg cggcaggccg ctacgatctt
     2881 tcacgccgaa agtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt
     2941 tccagtcgtc agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag
     3001 ccacgcagag tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc
     3061 cagacaggga gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac
     3121 gacgacgacg acccgtagtt gcaccgaatt cgttaccctg cttgcagagg aactcgccag
     3181 tttcatcaaa cagttcggcc gggaaaggac ctgcacctac acgagttgag taagctttga
     3241 ggatacccag aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac
     3301 cagcagtggt gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg
     3361 taccctgcgc accttcgaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt
     3421 cagaaacgtc aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa
     3481 ctttctggta atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt
     3541 ccatcacttc tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca
     3601 gaccgcgacg tgccacttta tcttcataag caggcccgat accacgaccg gtggtgccga
     3661 tcgctttcgc gccacgcgct ttctcacgcg cgttatccag agcaacgtga taatcaagga
     3721 tcagcggaca tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca
     3781 gttctttcat ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc
     3841 tggttacatt ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt
     3901 tgattacgag agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc
     3961 gttcagtcag aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta
     4021 cgacgacgtt gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc
     4081 gctttttcag agttacagca ctttttgcac tcaaaaatga cctcgatcag tctgtttttt
     4141 gttcagccaa tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca
     4201 ccgccaaaac gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc
     4261 catgccttcg ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc
     4321 agccagattg tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg
     4381 aggatgcggt ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc
     4441 ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg
     4501 ccagagaagc tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct
     4561 ttactgaatg catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcataatg
     4621 cggccctgac gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc
     4681 agtttttccg cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg
     4741 gcgcgcatac ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata
     4801 cgcacatcga caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact
     4861 ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atctgctgcc
     4921 ggggtagtaa cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg
     4981 agggtcagac gaccacggga atcggtgacg atatctttca cgtccaggcg accaatttca
     5041 gaacgcagac ggtcagagaa cttacgtttc aacagcactt ccgcttgcga aatgtcgcca
     5101 ccacccgttg ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagagtcg
     5161 acgatcaggt ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga
     5221 atacgtgcgt cgagcatttt caccgtttca atgaacggta tcttgaaatg cagaccaggc
     5281 tcataaacca aaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata
     5341 ccgcgctcac cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg
     5401 ataatcgcga taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct
     5461 gcgcgttggc gcggcgttgg tccataatat cgccctgact ggtggacgac gtgttgcttg
     5521 ctccactggt tgtggaggaa gaggctggcg gcagacgcag cagattgctg gcaccgttat
     5581 cgctcttcgc cgcaggggcg ttaccacctt tcagcatctg gtctaacggc agaaccatca
     5641 ggttgccacc tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg
     5701 tctcgatata cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt
     5761 tagcaaagcg cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg
     5821 cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggaatacg
     5881 cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg
     5941 ccgcttttac ttcttccggc ggacgagcag cctggaagtt gacgtccagc agcgtgatac
     6001 ccatgtcata cggacgaatc gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg
     6061 tacgaccttc cgtcagaatg cggtccatgg tgtatttacc gataactcca cgcagggcgc
     6121 tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg
     6181 gattggtgac gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca
     6241 gcatcacacc agaagcggcc agttcacgca cggcttccac gttcaccggt ttgacttcgt
     6301 cgataaacgt cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc
     6361 gtgttaccac gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa
     6421 tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt
     6481 gcgatgaact gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt
     6541 tgctcagttt gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt
     6601 ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc
     6661 cgttattacc gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc
     6721 ctgttgtctt tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc
     6781 aggtaatcga tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata
     6841 cgaacttgca gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc
     6901 tgataaaaac gactcctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc
     6961 acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc
     7021 tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg
     7081 aaatcttcca gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg
     7141 atctcttcaa gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca
     7201 tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgcc
     7261 gccaccagat cgtgcggcag gtggcgaata aaccctacgg tatctgcaag tacggtttca
     7321 ccgacatctg caacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct
     7381 gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta
     7441 tatcccacca gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca
     7501 cgctgctttt caactctttc caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa
     7561 cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt
     7621 ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc
     7681 tcaacctgca acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg
     7741 ccggtgcggt cgataacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg
     7801 ctcagggcat ggtcaaaaag aacgaccgaa gcacccatcg ctttgacagc ttccgcaatt
     7861 tcaactgctt taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta
     7921 atcacctgca atgcttcgac accggcggaa gagaccagag attcaaactc ctggaggtct
     7981 tccatatctt tgtcttgcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca
     8041 taacggtcaa acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg
     8101 ctccccgtgt aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
     8161 gaagtattct gcgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
     8221 ttactgtgat gagaaaccgg gcgagatggg acaacagtag aaatcgcgtg cttgtaaacc
     8281 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
     8341 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
     8401 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
     8461 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
     8521 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
     8581 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
     8641 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc
     8701 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
     8761 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
     8821 aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
     8881 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
     8941 cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
     9001 tgctgccgca accggatcta cctcctgaag ttgacgatgc aatgactccc aaccttgctc
     9061 tgccgcctgt tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa
     9121 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
     9181 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
     9241 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
     9301 gttcggcttc gccgtcccga tatccatccc tttgtaaata aggacagaat caacgcttat
     9361 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
     9421 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
     9481 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
     9541 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
     9601 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
     9661 atacggactg cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt
     9721 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
     9781 tacccaattc cgctagggca gactgcgctt tttctaatgc cgatttttct tcgccagaaa
     9841 cttttagccg caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca
     9901 attgtgcctg acgcagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat
     9961 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    10021 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    10081 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat
    10141 tcggccaggg ggcagccgga cgactgcctg aggcgggcgc aggagagtag cgcggagcta
    10201 ccggctcacg agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg
    10261 ggatggcacg cggtgcgggt tgtggttcat cgtccagcgg tagcggcgtt tccagttgct
    10321 gttgtagcac gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga
    10381 aacgcacttc gtgtttggcg gggtgtacgt tgacgtccac ctgatgcggg tcgatctcca
    10441 gatacaacac aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga
    10501 tcgcatgatt gatcaggcga tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt
    10561 ccgccagtgc gggcgtagtg tgatttggat cggccaccca gccgcgcagg gtgagatcgc
    10621 cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta
    10681 agcgccgttc tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt
    10741 tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatttcat
    10801 caatatggct aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    10861 agaacagatc cagcacctcc agcgtcgttc ccaccggatg cgctgccggt ttaaccgtca
    10921 cgtccatgtc gcgtccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    10981 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    11041 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgagcca
    11101 gcgccagcgc cagctcatct tttttgatac cgcagccgtt atcacgaatg cggataagtt
    11161 tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt
    11221 ccactagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    11281 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    11341 gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc accctgcggc gcagattgca
    11401 tcggatgcgc aaggaagtaa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    11461 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    11521 ccagtactga tgggatatcc ggcgagcgca gaacgccaag gctggcgtgt tctggtcgac
    11581 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    11641 ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    11701 actgactgtt cgccagcaca tcacccgccc cacccagcag ctccgacagt ttctcgtgct
    11761 gctccagcca gctggccatt tcactgttgg cgcgacggtt agagagcacc catacggaag
    11821 cgccagtcgc actacggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    11881 tttgcttatg tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    11941 ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    12001 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    12061 cgtgtccggc atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg
    12121 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    12181 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt
    12241 tcgcaaccac gggcggcgga ggaggcggtg gcggcacatc ggcgttaatc gtaaagacga
    12301 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat
    12361 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc agagcgaatc gccttcacca
    12421 gattattgcc gctgaacaac aacggcagtc cctgaatcat gcccgtttgt ttgatatcga
    12481 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    12541 acgttatccg cgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac
    12601 ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca
    12661 tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg
    12721 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    12781 ccgggtcagg aagaacacct gtaccttgtt gtggccactc caccaggcag atggcatcgt
    12841 tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca aggcggtaca
    12901 aatcaaagtg atagaccatt aagttgtcga gcgtataggg ttcgaccagc gtataagtgg
    12961 ggcttttgac attaccctga tgacccagag cctgtaaaaa gccccggcta aaggtggttt
    13021 tacctgcgcc taaatcgcca tacagataga ttacggttgc gccatcgcag gctttcgcta
    13081 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    13141 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    13201 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg
    13261 acacagcctg cacaggctgc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    13321 ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata
    13381 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    13441 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    13501 acggaacagc ctaacaaccg tgcggcctcg ctaggatgcg gcgtgatcac gcgattgtga
    13561 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    13621 cgaaaattct caactttttg cagggctttt ttcccccact cttgctggcc cagaccggga
    13681 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat agtcagttca
    13741 tgcaccatca attctggtcg tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt
    13801 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    13861 gccgtgccgt gatcgccacc gataatcacc agccgcccgt gatcgccttt atgcgaagtc
    13921 gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    13981 tcctgacctg ccagccagct atccagcccc agtgagtcaa aatgcagttg tcctgtaaca
    14041 tcccgcgctt ttccagtgag caagcctagt ttcagcgcaa taaaagtgat ggtgtgatcg
    14101 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    14161 accgccacaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    14221 cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg
    14281 ggccagacaa tattcgaagc atggatctcg ccacccgcgt ttaaccatgc ttcgcgtgcc
    14341 agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccaacaacgt gacctcaatg
    14401 ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    14461 cacagcacca gccagtggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggcc
    14521 tcgccagcgc gaagcatcag ctcatagagt gtgagcccca gcacatctgc cgcctcgcgt
    14581 tctccgcggc ggatatcgtc ggcgtaccag acggtgtgtg gtatacttac ggggtttttc
    14641 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    14701 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    14761 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    14821 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattacccgg tacgctgcgt
    14881 gtaatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    14941 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    15001 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    15061 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    15121 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta
    15181 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc
    15241 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    15301 gatgctcgcc gctgtatctc ttatctcacc atcgaacttg aaggggcgct cccggaagag
    15361 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    15421 tggaatcgct attcgcaact cactacagaa gacgatttca gcccgcgtaa gccgctacac
    15481 gcaccggaac tcattgagtt atttgcctgg agcgaagaga agtttttaaa agtcacggaa
    15541 ggatcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc cgtggcatta
    15601 ggcaatgccc cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac
    15661 ccacttctcg atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg
    15721 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg
    15781 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    15841 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cggaatcttg atttttaact
    15901 tatttatcaa taggttaaaa aaataccatt caccgtgcga agtttctttg aaattaagat
    15961 tacagcctga ggctgtggat aactctgttt acaagagttt gtcagagaca acaaaaacca
    16021 gcaataacgc atcgcgtcgc tgtagatatt ttattgagag aaga
//
LOCUS       DAJTRN010000087        15517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRN010000087 DAJTRN010000000
VERSION     DAJTRN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO328_002982"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO328_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5931464.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO328_002983"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO328_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5931465.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO328_002984"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO328_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO328_002985"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO328_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5931466.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO328_002986"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO328_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5931467.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO328_002987"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO328_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5931468.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO328_002988"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO328_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5931469.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO328_002989"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO328_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5931470.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO328_002990"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO328_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5931471.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO328_002991"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO328_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5931472.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO328_002992"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO328_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5931473.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO328_002993"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO328_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5931474.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO328_002994"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO328_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5931475.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO328_002995"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO328_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5931476.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO328_002996"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO328_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5931477.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO328_002997"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO328_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5931478.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO328_002998"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO328_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5931479.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO328_002999"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO328_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5931480.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO328_003000"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO328_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5931481.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO328_003001"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO328_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5931482.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO328_003002"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO328_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5931483.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO328_003003"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO328_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5931484.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO328_003004"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO328_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5931485.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO328_003005"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO328_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5931486.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO328_003006"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO328_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5931487.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO328_003007"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO328_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5931488.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO328_003008"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO328_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5931489.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO328_003009"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO328_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5931490.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO328_003010"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO328_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5931491.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO328_003011"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO328_003011"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5931492.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO328_003012"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO328_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5931493.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4006 a         3641 c         4130 g         3740 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctaaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaagccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctg
//
LOCUS       DAJTRN010000088        15490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRN010000088 DAJTRN010000000
VERSION     DAJTRN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(148..1257)
                     /gene="anmK"
                     /locus_tag="OO328_003013"
     CDS             complement(148..1257)
                     /gene="anmK"
                     /locus_tag="OO328_003013"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5931494.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1531..1998
                     /gene="slyB"
                     /locus_tag="OO328_003014"
     CDS             1531..1998
                     /gene="slyB"
                     /locus_tag="OO328_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5931495.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2045..2479)
                     /gene="slyA"
                     /locus_tag="OO328_003015"
     CDS             complement(2045..2479)
                     /gene="slyA"
                     /locus_tag="OO328_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5931496.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2680..2916
                     /locus_tag="OO328_003016"
     CDS             2680..2916
                     /locus_tag="OO328_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5931497.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2919..3776
                     /locus_tag="OO328_003017"
     CDS             2919..3776
                     /locus_tag="OO328_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5931498.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3776..5788
                     /locus_tag="OO328_003018"
                     /pseudo
     CDS             3776..5788
                     /locus_tag="OO328_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5789..6310)
                     /gene="sodC"
                     /locus_tag="OO328_003019"
     CDS             complement(5789..6310)
                     /gene="sodC"
                     /locus_tag="OO328_003019"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5931499.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6391..7287)
                     /locus_tag="OO328_003020"
     CDS             complement(6391..7287)
                     /locus_tag="OO328_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5931500.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7336..7575)
                     /locus_tag="OO328_003021"
     CDS             complement(7336..7575)
                     /locus_tag="OO328_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5931501.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7678..8277
                     /gene="nemR"
                     /locus_tag="OO328_003022"
     CDS             7678..8277
                     /gene="nemR"
                     /locus_tag="OO328_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5931502.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8314..9411
                     /gene="nemA"
                     /locus_tag="OO328_003023"
     CDS             8314..9411
                     /gene="nemA"
                     /locus_tag="OO328_003023"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5931503.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9492..9899
                     /gene="gloA"
                     /locus_tag="OO328_003024"
     CDS             9492..9899
                     /gene="gloA"
                     /locus_tag="OO328_003024"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5931504.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10002..10649
                     /gene="rnt"
                     /locus_tag="OO328_003025"
     CDS             10002..10649
                     /gene="rnt"
                     /locus_tag="OO328_003025"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5931505.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10742..12856
                     /locus_tag="OO328_003026"
     CDS             10742..12856
                     /locus_tag="OO328_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5931506.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12866..14158
                     /locus_tag="OO328_003027"
     CDS             12866..14158
                     /locus_tag="OO328_003027"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931507.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15108..15200
                     /locus_tag="OO328_003028"
     CDS             15108..15200
                     /locus_tag="OO328_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5931508.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15244..>15490)
                     /locus_tag="OO328_003029"
     CDS             complement(15244..>15490)
                     /locus_tag="OO328_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5931509.1"
                     /translation="VLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVN
                     GARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3549 a         4099 c         4072 g         3770 t
ORIGIN      
        1 ccggagagaa ggaccggcaa aatgataatt aacagttttt tcatagtcat atcccgaaga
       61 ctttcctggt ccggagggca aataccctcc ttaatcttcc aagtgtaacg gcagacacga
      121 taagaaaaaa ttcagttaat tctgatatca cgagttggcg gggaaaatag cccccagtac
      181 cgtctcctgg cttgcgccag tgacggaagg cagatttcct ggtaatcccg ccagcgtccg
      241 ccaggcaagc caggcgaaag ccaatgcttc catgtcatcg ccactaatgc caacggcatc
      301 ggtggtggtg acttctgtgc ctagcagtaa taccgccaga cgcgccatga gtagcggatt
      361 acgactacct ccaccacata ccatcaatcg ttcgcagcca ccgctcaaca aaacttgttc
      421 agaaatggtc acggcggtga gttctgccag tgtggcctga acatcacggg gatcaacacc
      481 cggaaaatgg cgtaaatggc gctccagcca gccgtagtta aagtattcgc gtccggtgct
      541 tttcggtgca ggttgcgaga aatacgggtc gctgagcata ttttgcagca gtgggagaat
      601 aactttaccc gcccgtgccc actcggcatc tttatcgtaa ggtttacctg cctgacgcca
      661 gatccaggca tccatcagca tgttaccagg accggtatcg tagccgccaa ccagctgccc
      721 aggaatgagc agtgacagat tggcgatgcc gccaatattg agcaccattc gtcgctcggt
      781 tgggtgagcc agcaaggcat gatggaacgc aggtaccagc ggtgcgcctt gtcctcccaa
      841 ggcaatatcg cgacggcgaa aatcaccgac aaccgtaatt ccggtgcgtg ccacaatttg
      901 attgttatcg ccaatctgta aagtgtgtgg tgccacgccc gtcggttcat gccagacggt
      961 ttgaccgtga cagccgatcg caactatatc tctcgcctgc aggttttgtt ccttaagcaa
     1021 ggcattgacc gcatcagcaa aaagtcgccc gagttgagta tcaagctgtc caaactgcga
     1081 aagtgtaagc tgctggccct ggcaaatatc cagtacagcc tgtttcagag acaccgggat
     1141 cggccagctc aagcttgcca gctgtgcgac ccggtgttca tcaattgtcg ccaacacaac
     1201 atcaacacca tcaaggctgg tgcctgacat aacgccaata aagcggcccg atttcatagt
     1261 tcatcctttt tcaatctgac gtttgcgcac cactcaaaca taaacttttc gtgaatacca
     1321 tgcggaatga ccgattttta ccgttggtag taaaacatta tcttcaaatc aataatcatc
     1381 atgaatgttt tgtttataat tggttgatcc tactttcatt atgatttgct catatttggt
     1441 agaacatgta accatggatt cacatatgcc atatactttg accatgaggg atgcttgcgt
     1501 ggcgtttcat ggtgaacagg agatttttca atgattaaac gcgtattggt tgtttcaatg
     1561 gtaggtctgt ctcttgtcgg ttgtgttaat aacgacaccc tgtcagggga tgtttatacc
     1621 gcttctgaag cgaaacaagt acagaatgtc agctatggca ccatcgttaa cgtacgtccg
     1681 gtacagattc agggcggtga tgattccaac gttatcggtg caattggcgg tgctgttctt
     1741 ggtggtttcc tggggaatac tgttggtggc ggaaccgggc gttctctggc tactgcagca
     1801 ggcgctgttg caggtggcgt agctggtcag ggcgtacaga gtgcaatgaa caaaacgcag
     1861 ggtgtcgagc tggaaattcg taaagacgat ggtaatacca tcatggtggt acagaaacaa
     1921 ggcaacactc gtttctctcc gggccaacgt gtcgtactgg ccagcaatgg cagtcaggtg
     1981 accgtttctc cgcgctaaat aagtttgcgt gtggtcaggt tactgaccac acgccccctt
     2041 catttcaccc tttggcctgt aactcaatga tattatgctc aagttttgcg atgagcgtaa
     2101 tcagttgctc cagttcctct gcggagatgc catgtaatat ttccgcgcgg gttttgttaa
     2161 taacagcttc catttcgctg atcagtggct ctgccttttc cgtcagttta atacgtttag
     2221 cccgacgatc gctggcacaa gtttgacgcg aaattaaccc tttttcttcc agttggtcca
     2281 gagtacggac cagtgatggc tgctcgatgc caatcgcttt tgccagttga atttgcgact
     2341 ggtctggagg taactgatgg atattgtgta acgtaaccca atgggtttgt gttaactcca
     2401 gcggtttcag gcgatggtct atcagagcac gccatatgcg caccaaccgt gccagatcag
     2461 aacctagtgg cgattccaat ttcatctcct tataattagc ttgctaagat attatgcggc
     2521 ttttagaata gtgtgcagca attgtattgc taaaacaaat gtattgctgc atttggttac
     2581 cgtcagacat atttttcaga aattgcgcgt aaatttttcg cacttaaaga atatttatta
     2641 atctaacgca atatattcgg tcgtaaagga atctactttg tgaagtttat gctcaatgca
     2701 acaggattgc ccttacaaga tctggtgttc ggtgcgtccg tctattttcc tccgtttttc
     2761 aaagcattcg cgtttggatt cgtcatctgg cttgtcgtac accgcctgct tcgtggctgg
     2821 atctacgccg gcgatatctg gcatcccttg ttaatggatt tatcgctgtt tgcgatttgc
     2881 gtttgccttg ctctggcaat actgattgcg tggtaactat gtcaattaaa acaattaagt
     2941 atttctcaac aatcattgta gcgatagttg cggttcttgc cggatggtgg ctgtggaatt
     3001 attacatgca atctccgtgg acacgcgacg ggaaaatacg cgcagaacag gtttctatca
     3061 ccccacaggt gtcgggacgt attgttgagc tgaatataaa agacaaccaa ctggttaacg
     3121 cgggggaact tctgctcacg atcgataaaa cgccctttca gatcgccgag ctgaacgcac
     3181 aggctcagct ggcgaaagcg caatctgacc tcgcaaaagc caataacgaa gcgaatcgtc
     3241 gtcgtcatct ctcacaaaat tttatttttg ccgaagaact cgataccgct aacctcaatg
     3301 ttaaagcgat gcaggccagt gttaatgccg cacaggcgac gctgaaacag acacaatggc
     3361 aactggcgca aacggaaatt cgcgccccgg tgagtggatg ggtgactaac ctcactaccc
     3421 gcatcggcga ttacgctgac accgggaaac cgctgtttgc ccttgtcgac agccactcgt
     3481 tttacgtcat tggttatttt gaagaaacca agttgcgcca tatccgcgaa ggtgcaccag
     3541 cacaaattac cctctatagt gataacaaaa cgttacaggg tcacgtttcc agtatcggtc
     3601 gggcgattta tgatcagagc gttgaaagtg actcaagcct tattccggat gtgaaaccta
     3661 acgtcccctg ggttcgtctc gcccaactcg ttcccgtccg ctttgcgctg gataaagttc
     3721 caggcgatgt cacgctggtg tcgggcacca cctgtagcat tgccgtaggt caataatgaa
     3781 cgcatcgtca tggtccttgc gcaatttgcc ctggttcagg gccacgctgg cgcaatggcg
     3841 ttatgcgtta cgcaatacca ttgccatgtg tctggcgctg acggttgcct attatttgaa
     3901 tctggatgaa ccctattggg cgatgacctc ggctgcagtg gttagctttc ccaccgttgg
     3961 cggtgtcatc agcaaaagcc tcggacgcat ggctggcagt ttactgggag ccattgcggc
     4021 actgcttctt gccggacata cgctcaatga gccgtggttt tttctattga gcatgtcggc
     4081 gtggcttggc ttttgtacct aggcctgtgc gcacttcacg aataacgtcg cgtatgcatt
     4141 tcaactggcg ggctacacgg ctgccatcat cgcctttccg atggttaata ttactgaggc
     4201 cagccagctg tgggatatcg ctcaggcgcg cgtttgcgag gtgatagtcg gtattttgtg
     4261 cggcggcatg atgatgatga tcctgcctag cagttccgat gctactgccc ttttaactgc
     4321 attgaaaaac atgcacgccc gattactgga acatgccagt ttactctggc agcctgaaac
     4381 aaacgatgcc attcgtgcag cacatgaggg ggtgattggg cagatactga ccatgaattt
     4441 gctgcgtatc caggctttct ggagccacta tcgttttcgc cagcaaaacg cgcgccttaa
     4501 tgcgctactc caccagcaat tacgtatgac cagtgtcatc tccagcctgc gacgtatgtt
     4561 gctcaactgg ccctcaccgc caggtgccac gcgagaaatt ctcgaacaat tgctgacggc
     4621 gctcgccagt tcgcaaacag atgcttacac cgtcgcacgt attatcgccc cgctacgccc
     4681 gaccaacgtc gccgactacc ggcacgtcgc cttctggcag cgactacgtt atttttgccg
     4741 cctttatctg caaagtagtc aggaattaca tcgtctgcaa agcggtgtag atgatcatgc
     4801 cagactcgca cggacatccg ctctggctcg tcataccgac aacgccgaag ctatgtggag
     4861 cgggctgcgt acattttgta cgttgatgat gattggcgca tggagtattg catcgcaatg
     4921 ggatgccggt gccaatgcat taacgctggc agcaattagc tgcgtactct actccgccgt
     4981 cgcagcaccg tttaagtcgt tgtcactgct gatgcgcacg ctggtgttac tttcgctatt
     5041 cagctttgtg gtcaaatttg gcctgatggt ccagattagc gatctgtggc aatttttact
     5101 gtttctcttt ccactgctgg cgacaatgca gcttcttaaa ttgcagatgc ccaaatttgc
     5161 cgcactgtgg gggcaactga ttgtttttat gggttcgttt atcgctgtca ctaatccccc
     5221 ggtgtatgat tttgctgatt ttcttaacta taatctggca aaaatcgttg gcgttgcgct
     5281 ggcttggcta gcgttcgcca ttctgcgtcc aggatcggat gctcgtaaaa gccgccgcca
     5341 tattcgcgcg ctgcgccggg attttgtcga tcagctaagc cgccatccaa cactgagtga
     5401 aagcgaattt gaatcgctca cttatcatca cgtcagtcag ttgagtaaca gccaggatgc
     5461 gctggctcgc cgttggttat tacgctgggg tgtagtgctg ctgaactgtt ctcatgttgt
     5521 ctggcaattg cgcgactggg aatcgcgttc cgatccgtta tcgcgagtac gggataactg
     5581 tatttcactg ttgcggggag tgatgagtga gcgtggcgtt cagcaaaaat cactggcggc
     5641 cacacttgaa gaattacagc ggatttgcga cagccttgcc cgtcatcatc aacctgccgc
     5701 ccgtgagctg gcggcaattg tctggcggct gtactgctcg ctttcgcaac ttgagcaagc
     5761 accaccgcaa ggtacgctgg cctcgtaatt acttaattac accacaggca tagcgttcac
     5821 cgccaccgcc cagcggttta ggttgatcgg acatattatc gccgccaacg tggaccatca
     5881 gcgccttgtc tttgatttca tccagtgatt tcagacgagg cgcgatgacg gcatcggtag
     5941 ctttgccgtc attattgacg accagtgcag gcagatcgcc taaatgcccg gtaccttctg
     6001 gcccttcatg tttaccggta ttttgtggat caagatgccc gcctgcggat tccgcggcgc
     6061 tggctttgcc atctttggtg gctggctggc agcttccttt ggcatgaata tggaagccat
     6121 gttcaccggg gggtaatgct ttcagatcgg gtgaaaactc cagaccttta tcggtttcag
     6181 taatggtgac gctaccaatt gactgcccaa ccccttgcga cgtgacgagg ttcatctcga
     6241 ctttttcact ggcagcttgt gcgccggtcg caacaaccag cgccagaatg gccagactaa
     6301 aacgtttcat aggacctccg ttcattgtgc attcctgaat aagtgtaaac cagtgacaca
     6361 tttttgaacg gcggctattc ctaaaagtgc ttacggtacg tcgtacccca gtgcagcttt
     6421 acggatacga aaccattgtt gacgggtcat tttcagtgtt tctgcttcga cagctgcccg
     6481 tacgcgctca attttacctg agccgataat tggcagcggc tgcgacggta aacgtaatac
     6541 ccaggcgtaa accacctgtt caatcgagcc cgcgtttaac tcctccgcca ccacagccag
     6601 ttcatcacgc agcggctgga aataatcatc attaaacaga cgaccaccac caaggcagga
     6661 ccacgccatc ggacgaacac gcagttgttg tagttggtcg agcgtgccat ccagcagtaa
     6721 cggctgatgc accggggata tttccacctg attagtggca agggtaaacg gcagacgtga
     6781 ttgcaacagg gcaaattgcg caggcgtaaa gttcgatacg ccaaaatgac gcactttgcc
     6841 gctctgatgc agatgtttga acgcgtccgc cacttcatcg gcatccatta acgggtctgg
     6901 tcggtggatt aacagcagat ccagatgatc ggtcgcgaga ttaattagcg actgttcggc
     6961 gctcttaatg atgtgattgc ggtcagtgat gtaatgacca atgacgtttt cttcacgcgc
     7021 ggtcgtcgcg ataccgcatt tactgacgat ttccatccgt tcacgcaggt gaggtgccag
     7081 tttcagtgcc tcgccaaacg ccgcttcgca ctgatagcca ccataaatat cagcatggtc
     7141 cacggtggtc acgccgagat ccagatgctc ttcaataaaa ctgaccagct ggcgggcgga
     7201 catattccag tccatcaatc gccagtagcc catcacaaaa cgggaaaact ccgggccttc
     7261 cggcgcaata gtaatacgct gaaccataat cgcttcctct tatcagatat aagaggagta
     7321 tacgcaagat taggttcaaa agagtgatgg ttgctccggt tcgtctgatg acgctggctt
     7381 atttgcgcgt aatttgcgca ttaatcgctg ccgacaaagg cgcagcacct cttgtttttc
     7441 gccatcgctc attttattcc agttaaaacg ctcatcccga ctacgaaaac agccgcgaca
     7501 aaacccgcgt tcgtcagact ggcaaattcc ccggcacggg ctctggacgg gaaagaactc
     7561 taattgctcc gccacctcgc cctcctcaga taagattatt accattattg aagctgttaa
     7621 tgtccaaagt agcaactttg cttgcactag accaactggt ctactacact ccaacgcatg
     7681 aacaaacaca ccgaacatga tactcgcgaa catctcttgg cgacgggcga gcaactttgc
     7741 ctgcaacgtg gattcaccgg gatggggcta agcgaattac taaaaaccgc tgaagtgccg
     7801 aaagggtcct tctatcacta ctttcgctct aaagaagcgt ttggcgttgc catgcttgag
     7861 cgtcattacg ccgcatatca ccagcgactg gctgagttgc tgcaatccgg cgaaggtaac
     7921 taccgcgacc gcatactggc ttattaccag caaacactga accagttttg ccaacatgga
     7981 accatcagtg gttgcctgac agtaaaactc tctgccgaag tgtgcgatct gtcagaagat
     8041 atgcgcagcg cgatggataa aggtgctcgc ggcgtgatcg ccctgctctc gcaggctctg
     8101 gaaaatggcc gtgagaacca ttgtttaacc ttttgtggcg aaccgctaca acaggcacaa
     8161 gtgctttacg cactatggct tggcgcgaat ctgcaggcca aaatttcgcg cagtttcgag
     8221 ccactggaaa acgcgctggc ccatgtaaaa aacattattg cgacgcctgc cgtttagcag
     8281 gcatttttta tcaccagacg accgggagcc tttatgtcat ctgaaaaact gtattcccca
     8341 ctgaaagtgg gcgcgatcac ggcggcaaac cgtattttta tggcaccgct gacgcgtctg
     8401 cgcagtattg aaccgggtga cattcctacc ccgttgatgg cggaatacta tcgccaacgt
     8461 gccagtgccg gtttgattat tagtgaagcc acgcaaattt ctgcccaggc aaaaggatat
     8521 gcaggtgcgc ctggcatcca tagtccggag caaattgccg catggaaaaa aatcaccgct
     8581 ggcgttcatg ctgaaaatgg tcatatggcc ttgcagctgt ggcacaccgg acgcatttct
     8641 cacgccagcc tgcaacctgg cggtcaggca ccggtagcgc cttcagcact tagcgcggga
     8701 acacgtactt ctctgcgcga tgaaaatggt caggcgatcc gcgttgaaac atccatgccg
     8761 cgtgcgcttg aactggaaga gattccgggt atcgtcaatg atttccgtca ggccattgct
     8821 aacgcgcgtg aagccgcttt tgatctggta gagctccact ctgctcacgg ttatttgctg
     8881 catcagttcc tttccccttc ttcaaaccat cgtaccgatc agtacggcgg cagcgtggaa
     8941 aatcgcgcac gtttggtact ggaagtggtc gatgccggga ttgaagaatg gggtgccgat
     9001 cgcattggca ttcgcgtttc gccaatcggt actttccaga acacagataa cggcccgaat
     9061 gaagaagccg atgcactgta tctgattgaa caactgggta aacgcggcat tgcttatctg
     9121 catatgtcag aaccagattg ggcggggggt gaaccgtata ctgatgcgtt ccgcgaaaaa
     9181 gtacgcgccc gtttccacgg tccgattatc ggcgcaggtg catacacagt agaaaaagct
     9241 gaaacgctga tcggcaaagg gttaattgat gcggtggcat ttggtcgtga ctggattgcg
     9301 aacccggatc tggtcgcccg cttgcagctc aaagctgagc ttaacccaca gcgtgccgaa
     9361 agtttctacg gtggcggcgc ggaaggctat accgattacc cgacgttgta atccaacatt
     9421 gcgagcggcg taaagccgcc gctatactaa aacaacattt tgaatctgtt agccattttg
     9481 aggataaaaa gatgcgtctt cttcatacca tgctgcgcgt tggcgatttg caacgctcca
     9541 tcgattttta taccaaagtg ctgggcatga aactgctgcg taccagcgaa aacccggaat
     9601 acaaatactc actggcgttt gttggctacg gcccggaaac cgaagaagcg gtgattgaac
     9661 tgacctacaa ctggggcgtg gataaatacg aactcggcac tgcttatggt cacatcgcgc
     9721 ttagcgtaga taacgccgct gaagcgtgcg aaaaaatccg tcaaaacggg ggtaacgtga
     9781 cccgtgaagc gggtccggta aaaggcggta ctacggttat cgcgtttgtg gaagatccgg
     9841 acggttacaa aattgagtta atcgaagaga aagacgccgg tcgcggtcta ggcaactaat
     9901 ctcctgccgg gcgtgaactc atcgcgcccg catctttact gcatcgacaa gtaatatttg
     9961 tcataatgcg cgctgcaatt tatccgtatt aagagaatca gatgtccgat aacgctcaac
    10021 ttaccggtct gtgcgaccgt tttcgtggtt tttatcctgt tgtgatcgat gttgaaacag
    10081 ccggatttaa cgccaaaacc gatgcgctgc ttgagattgc cgccatcacc ctgaaaatgg
    10141 atgaacaagg ctggctgatg ccggacacca cattacattt ccacgtcgaa ccatttgtcg
    10201 gcgcaaattt gcaaccagaa gccctcgcct tcaacggcat tgacccgaac gatcccgatc
    10261 gcggcgcggt cagcgaatac gaggcgctgc acgaaatttt taaagttgta cgtaaaggta
    10321 ttaaagcgag cggctgtaac cgcgccatta tggtggcgca caacgccaat tttgatcaca
    10381 gctttatgat ggccgccgca gaacgcgcct cgctgaaacg taacccgttc caccctttcg
    10441 ccacttttga cactgctgca ctggccgggc tggcactcgg acaaaccgta ttgtcaaagg
    10501 cttgccagac cgctggcatg gacttcgaca gcacccaggc gcactccgcg ctgtacgaca
    10561 ccgaacgcac tgctgtgctg ttttgtgaaa tcgtcaaccg ctggaaacgt ctgggaggct
    10621 ggccgctacc tgccgccgaa gaggtgtaat cgagtcgatg cctgatgaca tgcaatgatt
    10681 caggcatcta tagtgaggct attccacgca tcaagcatga tattcacggg gaatagcgtt
    10741 aatggcagat aatccagacc cttcatcgct cctgccggac gtgttttcac cggcgacccg
    10801 cgactggttt cttcgcgcct ttaaacagcc gaccgctgtc cagtcgcaaa cctggcatgt
    10861 ggcggcgcga ggcgaacatg cgctggtgat tgcaccgact ggctccggga aaacgctggc
    10921 agcatttctc tacgccctcg atcggctctt ccgcgaaggc ggcgaagata cccgcgaggc
    10981 gcataagcgt aaaacctcac gcatcctcta tatttcaccg ataaaagccc tgggcaccga
    11041 cgttcagcgc aacttgcaga tcccgttgaa gggtattgcc gatgaacggc ggcggcgcgg
    11101 cgaaacggta attattgatg aagtccacgc ggtagcgggc agtaaacgtg gtgcgcatct
    11161 ggcgttaagt ctggagcggc tcgatgcgct gctccacacc tcagcacagc gaattggcct
    11221 ttctgccact gtgcgctcag ccagcgatgt ggcagcattt cttggtggcg atcgcccggt
    11281 tacggtagtc aacccgccca caatgcgcca tccgcagata cgaattgtcg taccggtcgc
    11341 caatatggat gatgtctcat cggtcgccag cggcaccggc gaagacagcc atgccggccg
    11401 ggaaggctcc atctggccat atattgaaac gggtatcctt gatgaagtgt tgcgccatcg
    11461 ctcgaccatt gtctttacta attcacgcgg gctggcggaa aaactgacgg cacgattaaa
    11521 tgagctttac gccgcacgct tacagcgttc cccgtctatc gccgttgatg cggcccattt
    11581 cgagtcgacc tccggcgcaa cctctaaccg tgtacaaagt agcgacgttt ttattgcccg
    11641 ctcacaccac ggctccgtct ctaaagaaca acgagcaatc accgaacagg cgctgaaatc
    11701 gggtgaatta cgctgcgtgg tcgcaacctc cagtcttgaa ctggggattg atatgggcgc
    11761 ggtggatctg gtgattcagg tggcaacgcc gctttctgtt gccagtgggt tacaacgcat
    11821 tggtcgcgcc ggacatcagg ttggcggtgt atctaaaggg ctgtttttcc cccgtacccg
    11881 gcgtgattta gtcgattccg cagtcattgt agagtgtatg ttcgcaggca ggctggaaaa
    11941 cctgacacca ccgcataatc ctctcgacgt ccttgcgcag caaaccgttg ccgccgcggc
    12001 gatggatgca ttacaggtag acgaatggta ctcccgcgta cgccgtaccg caccgtggaa
    12061 agatctgcca agacgtgttt ttgacgccac gctggatatg ctttccgggc gctatccctc
    12121 tggcgatttt tctgcttttc gccccaaact ggtctggaac agggagaccg ggatattgac
    12181 cgcccgacct ggcgctcaat tgttggcggt taccagcggc ggcaccattc cggatcgtgg
    12241 catgtatagc gtgttattac ccgaaggtga agaaaaggcc ggttcgcggc gggtgggtga
    12301 actggatgag gagatggtat atgagtcgcg ggtgaacgac attatcactc tcggcgctac
    12361 ctcatggcgg atccagcaaa tcacccgcga tcaggtgatt gtgactcctg ctccgggtcg
    12421 ttctgcccag ctccccttct ggcgtggtga aggtaacgga cgtccggctg aattaggcga
    12481 gatgatcggc gattttcttc atttactggc ggatggcgcg ttcttttccg ggactattcc
    12541 cccgtggctg gcagaagaaa atacgaacgc caatattcag gggttgattg acgagcaacg
    12601 caacgcgacg ggcatcgttc cggggagtcg ccatctggtc ctcgaacggt gccgtgatga
    12661 aattggcgac tggcgtatta ttttgcactc tccctatgga agacgggtgc atgaaccctg
    12721 ggcgctggcg attgccgggc gaatacatgc cctatggggc gctgacgcgt ctgtggtcgc
    12781 cagtgatgac agcattgttg cgcgcattcc tgacactgat ggtaaattgc ccgacgccgc
    12841 gatttttttg ttttaaccag aaaagttgct gcaaattgtc cgcgaggcgg taggcagctc
    12901 ggcacttttc gccgcccgtt ttcgcgaatg cgccgcgcgg gcattattaa tgccgggccg
    12961 cacgccgggc catcgcaccc cgctttggca acaacggctg cgcgccagtc agttgctgga
    13021 aatcgctcag ggatatccgg attttccggt cattctcgaa accctacgcg aatgtctgca
    13081 agatgtttat gatcttcccg cactggaacg tttgatgcgt cgcctgaacg gtggcgaaat
    13141 tcaaatatcc gatgtaacga ccactacgcc ctcgcctttc gccacaagtt tattgttcgg
    13201 ctatgtcgcg gaatttatgt accagagcga cgccccgctg gcagagcgcc gggcatccgt
    13261 actgtcgctg gacagcgagt tactgcgcaa tctactcgga caggtcgatc cgggggaatt
    13321 actcgacccg caggtcattc gccaggtgga agaagagttg caacgactgg ctcctggtag
    13381 aagagcgaaa ggtgaagaag gattgttcga cctgctgcgc gaactggggc caatgaccgt
    13441 tgaagacctg gcgcaacggc atacaggcag cagtgaagag gttgcgtcgt atctggaaaa
    13501 tcttcttgca gtaaaacgaa tcttcccagc gatgattagc ggacaggagc gtcttgcctg
    13561 tatggatgat gccgccaggc tgcgtgatgc cctcggcgta cggctaccag agtcattgcc
    13621 agagatttat ttacatagag tcagttaccc gcttcgcgac ctctttctgc gctatctccg
    13681 ggctcatgct ctggtcacgg ctgaacaact ggctcatgag tttagtctcg gtattgccat
    13741 tgtcgaagag cagcttcagc aactgcgtga acagggtctg gtgatgaatc tgcaacaaga
    13801 catctgggtg agcgatgaag tatttcgtcg tctgcgtttg cgctcgctgc aagccgccag
    13861 agaagcgacg cgtcccgttg cagccacgac ctatgcgcga ttgctgctgg aacgtcaggg
    13921 cgtattaccc gccaccgatg gtagcccggc gctctttgcc tcaacatctc caggcgttta
    13981 tgagggcgta gatggagtga tgcgggtgat cgaacagctt gccggagtcg gtttacccgc
    14041 ctcactctgg gaaagccaga tcctgcctgc ccgcgtacgc gactattcgc cagaaatgct
    14101 cgatgaatta ctggcaaccg gagcggttat ctggtcggga caaaaaaaag ctgggtgaag
    14161 atgacggcct ggtggcactg catctacaga aatatgccgc agaatcgttc actcccgccg
    14221 aagcggatca ggcgaatcgt tcggcgctgc agcaagcgat aatcgctgtt ctggctgacg
    14281 gaggagcctg gtttgcacaa caaatcagcc aacggatacg cgataaaatc gtcgaatcgg
    14341 ttgattcctc ggcactgcaa gaggcgttat gggcgctggt ctggcaaggc gtcattacca
    14401 gcgatatttg ggcaccgtta cgcgccctca cccgcagcag ttccaacgca cgcacctcaa
    14461 ctcgccgcag tcaccgggct cgtcgtgggc gtcctgtcta tgcacaaccc gtctcgccgc
    14521 gggtatctta caacacacca aatctggctg gacgctggtc gttattgcag gtggagccac
    14581 taaacgatac cgaaaggatg ctggcgctgg cggaaaatat gctcgaccgc tacggcatca
    14641 tcagtcgtca ggcggtgata gccgaaaata tccctggcgg gtttccatcg atgcaaacac
    14701 tttgtcgaag tatggaagac tccgggcgaa ttatgcgagg tcgttttgta gaaggtctgg
    14761 gtggcgcgca attcgctgaa cgtctgacta ttgaccgatt gcgcgatctg gcgacacaag
    14821 ccacgcaaac acgccactat acaccagtgg cgctctccgc caacgatccg gctaatgtgt
    14881 ggggaaatct tctgccctgg gctgcacatc cggcaacgct ggtaccaacg cgtcgggcgg
    14941 gtgcgctggt ggtcgtttct ggcggcaaat tgttactcta tctggcgcaa ggcggcaaaa
    15001 aaatgctggt ctggcaggaa aaggaggaat tactcgcccc agagattttc caggcgctga
    15061 ccaccgcact gcgtcgcgaa ccaaggctgc gctttacgct aacagaagtg aatgatcttc
    15121 cggtccggca aacgccgatg tttacgctgc tgcgtgaggc gggattttca agttcgccac
    15181 aagggctgga ttggggatag ttgacatcct ccacgccctg aaagacgtgg ttccctgcta
    15241 cgttcaagct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc ggcctgactg
    15301 caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagcgccatt
    15361 tacatcggcg ttcgctgtat atccacatac ctggcatctg aatttacttt gtgacaggcg
    15421 gttttctttc gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg ctggcggaac
    15481 agcaagcacc
//
LOCUS       DAJTRN010000089        15408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRN010000089 DAJTRN010000000
VERSION     DAJTRN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1173)
                     /locus_tag="OO328_003030"
     CDS             complement(<1..1173)
                     /locus_tag="OO328_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5931510.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="OO328_003031"
     tRNA            complement(1372..1456)
                     /locus_tag="OO328_003031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="OO328_003032"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="OO328_003032"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5931511.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="OO328_003033"
     CDS             2800..4302
                     /locus_tag="OO328_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5931512.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="OO328_003034"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="OO328_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5931513.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="OO328_003035"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="OO328_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5931514.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="OO328_003036"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="OO328_003036"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5931515.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="OO328_003037"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="OO328_003037"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5931516.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="OO328_003038"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="OO328_003038"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5931517.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="OO328_003039"
     CDS             complement(11867..13063)
                     /locus_tag="OO328_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5931518.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="OO328_003040"
     CDS             13256..13759
                     /locus_tag="OO328_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5931519.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="OO328_003041"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="OO328_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5931520.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="OO328_003042"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="OO328_003042"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5931521.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3932 c         3968 g         3532 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcggcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcgcc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt caggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatgacg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat cacaccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatccg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttattgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaggt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccaca caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgagtttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgaa
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DAJTRN010000090        15229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRN010000090 DAJTRN010000000
VERSION     DAJTRN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..275)
                     /locus_tag="OO328_003043"
     CDS             complement(<1..275)
                     /locus_tag="OO328_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931522.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVT"
     gene            complement(728..1990)
                     /locus_tag="OO328_003044"
     CDS             complement(728..1990)
                     /locus_tag="OO328_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5931523.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(2188..2263)
                     /locus_tag="OO328_003045"
     tRNA            complement(2188..2263)
                     /locus_tag="OO328_003045"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2228..2230),aa:Phe,seq:gaa)
     gene            complement(2369..3076)
                     /locus_tag="OO328_003046"
     CDS             complement(2369..3076)
                     /locus_tag="OO328_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5931524.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3474..5609
                     /gene="speC"
                     /locus_tag="OO328_003047"
     CDS             3474..5609
                     /gene="speC"
                     /locus_tag="OO328_003047"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5931525.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5658..6914)
                     /gene="nupG"
                     /locus_tag="OO328_003048"
     CDS             complement(5658..6914)
                     /gene="nupG"
                     /locus_tag="OO328_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5931526.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7116..8195)
                     /gene="mltC"
                     /locus_tag="OO328_003049"
     CDS             complement(7116..8195)
                     /gene="mltC"
                     /locus_tag="OO328_003049"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5931527.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8260..8535)
                     /locus_tag="OO328_003050"
     CDS             complement(8260..8535)
                     /locus_tag="OO328_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5931528.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8563..9615)
                     /gene="mutY"
                     /locus_tag="OO328_003051"
     CDS             complement(8563..9615)
                     /gene="mutY"
                     /locus_tag="OO328_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5931529.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9776..10495
                     /gene="trmB"
                     /locus_tag="OO328_003052"
     CDS             9776..10495
                     /gene="trmB"
                     /locus_tag="OO328_003052"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5931530.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10495..10821
                     /locus_tag="OO328_003053"
     CDS             10495..10821
                     /locus_tag="OO328_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5931531.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            11005..11724
                     /locus_tag="OO328_003054"
     CDS             11005..11724
                     /locus_tag="OO328_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5931532.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11900..12946
                     /gene="ansB"
                     /locus_tag="OO328_003055"
     CDS             11900..12946
                     /gene="ansB"
                     /locus_tag="OO328_003055"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5931533.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13063..14025
                     /locus_tag="OO328_003056"
     CDS             13063..14025
                     /locus_tag="OO328_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5931534.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(14126..>15229)
                     /locus_tag="OO328_003057"
     CDS             complement(14126..>15229)
                     /locus_tag="OO328_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR5931535.1"
                     /translation="FIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANMMFG
                     SISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTA
                     GGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDALPSL
                     LLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTTSIV
                     LLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAV
                     LIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIK
                     PLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3977 a         3667 c         3891 g         3694 t
ORIGIN      
        1 gtaacaatat caggcgcgcc tgcggaacgg tccttaccat gctgcagtaa ccggaaataa
       61 acttttgggc gcaagatgac atcgaccgtt tcgacatcgt ctttttctcc ctcaaaaaat
      121 ttactgacaa tcgcttcgtc aagacgtccc gccgctatcc catgccagtg agtgaaattt
      181 tgggcgtctt tccgtttaaa actgcccttt cctgactcag catccgcaag cgagttgcgc
      241 cagtatgagg caaaccctaa agcattttca tccattacca gtcctgtgac tcaatgaaat
      301 ccattgattt atcgaatata aaatcaccgt atggacagct attgtccata ttgaaaaagt
      361 tggatgcaat gttttgttta taattcgata tcaaatgaac tggttgtaca aggttcagaa
      421 cacaagctca ttaaacagta cgtcaggact attgatttca taacagtgaa tacatttttt
      481 accgtttata ataactacct gtgttggacg gggataccag agcagccgaa aacaccgggg
      541 gttattacct gctctcatgg aaaccatata ttcctggtgc taatcacagt cataaatagc
      601 ctgaagaacg tgacgaaggg actttaatgg aagccggagt aacttgtcag aaacatcaat
      661 cttcgtcatc atttacaaag aaaaataatt agttaaagca gtccgggctg attgcccgga
      721 ctgcttacta ttttaatgga ttgttaatct ttgcatattc aaacgggctg atacccttct
      781 cccggttcgc atctagaaaa tcggcccacc attgcaacat cagtcgccgt tcttccagat
      841 gttctgcttt atggatatac gcagcacgta caccattacg ctcctgatgg ctcatctgac
      901 gttccacagc atcgcgggac cacaaacctg attcaatcaa tgcactgcac gccatcgccc
      961 ggaatccatg gccacagact tcctgggttg tgtcataccc catcaccctc agggctttat
     1021 ttacagtatt ttcactgatt ggtttacgcg ggtcatgtgc acccgtaaag atcagaccat
     1081 tttcaccagc ccatgtctga agttctgcca gtatggctac aacctgtttg ctgagtggca
     1141 caagatgtgg tgttcgcatc ttggatcccc ggtgtgagaa ttttacgcct ggcagaggtt
     1201 cgcgttcggc tggaatagtc cacatggaat tgtcaatatc aatttccgac cagcgggcat
     1261 atctgagttc actggaacga ataaaaacca gtaaattcag cataactgcc agtcgggtga
     1321 caggctggcc tttataaccg tcaatacgcg acagaagttc aggcaggcgt gaaagctcca
     1381 gagcagggcg atgctggcgt tttaccgtgg tcaatgcacc agccatatcc agagccgggt
     1441 tatatctgat gatgccagac tgtacggcgt aacgcattac agcagatatt ctttgctgca
     1501 gacgactggc gatttcataa atttgttttg cttctgcagc acgaacaggg ataagcaggt
     1561 ccggggtatt gagtgttgtg atatcccggt tgccgataaa ggggaaaacg tgtgtctcga
     1621 ggcttttgag tactttatcg ctgtgtgatt gtgcccattg tttattactg gcatgccagc
     1681 gtcgtgcaat atgttcaaaa gagagctgta aatcagggac aactttttta actcttttct
     1741 gttcgcaagg gtcaatcccc tctgaaatca gttttcgggc ctcatcccgt tttgtacgag
     1801 cttcggagag agaaacttct ggatacacac caagtgccag agtcttctcc ttacccagaa
     1861 tacgataacg gagacgccag taacgggaac cattaggatg aaccagcagg aacatgccgg
     1921 ccccgtcagt gagtttataa gccttgtcag tgggctttgc agcccggatt tttgcgtcag
     1981 tcagtgccat agttcagcct tctgaatgcg ttaaagaggg tacaaattct gtcgaactgg
     2041 ggtatacccg caattatacc cacacaatgg ggttgatgtg gggggaatct ggttgagttc
     2101 ggtagaattg atttggagga agaacactta aatcatggtg ttctgaaacg aaaaacggac
     2161 ctccgtggag gtccgttgat atgaatttgg tgcccggact cggaatcgaa ccaaggacac
     2221 ggggattttc aatcccctgc tctaccgact gagctatccg ggcaacgggg cgcattaaac
     2281 ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta ctgcagactg cacaatctat
     2341 catcactttg ccgctattgc acgcattgtc aggcaaacca ggcggtccag gcggcggaaa
     2401 gcggcattgc cagaaggagt gctggaagca tgttgaccac cgggaacatt ttaatgccac
     2461 agatgcgtaa tccggttgcc agcagcaata aaccgcctac ggcgctgaag tctgccatca
     2521 tcgacggtgt gatcagcggt aatatcagcg cggcagccca cgccagcgtt aactggatga
     2581 tcagtaatgg gatactaatt accgacaccg caatacccag cgagcaggcg aagatcatcg
     2641 ccgtaaagaa atcaagaaat gacttggcga ttaaaatact cggatcgccg gtcatccctt
     2701 cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa caggacaata atcgcgacat
     2761 aattctgaat aaaagattca tgcgctggct tcttacgtga gtgacgaaac agattttgtg
     2821 ctttggcgac tgctgtattg acacctttct ccagcaaaca aatttcgcca attagagcgc
     2881 caagtagcgt agctaaaacc atcgctggaa ggttggcgca tttcacaacc agtaaaatac
     2941 caatccccag cgatgccaga ccaaaaatag atgtcatgga gacgcggata cgttccggta
     3001 agcgttggct gagcagtgcg ccgagaacgc cacccagtaa gactgcacta gcgttgataa
     3061 aagggccgat gaccacaaga gttcctgtta gctgtattgt ttgattttct tattatgaac
     3121 tttttgcctt gttgatggct tgtctggtcg caaagtcctg gcttgcacgc cttagcgaaa
     3181 ggtgccatga ttgcgcgaat tttctcctct ctgtacggag tttgcccgat gcacgccatc
     3241 tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg tttccctttc tatgttactg
     3301 ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca gagcgtttct
     3361 gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccatat ctcatggggc
     3421 agggttttcc accttgtccg gtattcttac ttccccgaaa cgggtttgcg cttatgaaat
     3481 caatgaatat tgccgccagt agtgaactgg tatcccgact ttcttctcat cgccgcgtgg
     3541 tggcgttggg agatactgat tttacggacg tcgcggcagt cgtcattacc gctgcggata
     3601 gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt tcatctaccg gtgtttttgt
     3661 attccgaaca tgctgttgaa ttacctgcgg gcgttacggc ggtaatcaac ggcaacgagc
     3721 agcagtggct ggagctggaa tccgcagcct gtcagtatga agagaatttg ctgccaccgt
     3781 tttatgacac gctgacgcag tacgttgaga tgggcaacag cacctttgct tgccctggac
     3841 atcaacatgg tgcgttcttt aaaaagcatc ctgccggacg ccatttttac gatttctttg
     3901 gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt aaaattgggc gatctgctta
     3961 ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc caaagtcttt catgccgata
     4021 aaacctattt tgtgctgaac ggcacatcgg cagcgaataa agtggtgacg aatgcgctgt
     4081 taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca taagtcgaat catcacggcg
     4141 cgctgattca ggcggggacg acgccggtct atctggaagc ttcacgtaac ccgtttggtt
     4201 tcattggcgg tattgatgcg cactgtttta atgaagagta tctgcgccag caaattcgcg
     4261 acgttgcgcc agaaaaagcc gacctgccgc gcccgtttcg cctgacgatt attcagctgg
     4321 gaacctatga cggcactgtc tataacgccc gtcaggtgat cgataccatt gggcatctgt
     4381 gtgattacat tctgtttgat tccgcgtggg tcggttacga acagtttatc tcgatgatgg
     4441 cggatagctc gccgctgctg ttagaactta acgaaaacga tccggggatt tttgtgaccc
     4501 agttggtgca caaacagcag gctggattct cacagacgtc gcagatccat aaaaaagata
     4561 accatatccg cggacaggcg cgtttttgcc cgcataagcg gttgaataac gcctttatgc
     4621 tccatgcttc taccagccct ttctatccgc tgtttgctgc actggatgtt aacgccaaaa
     4681 ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg cgttgagttg ggtattgagt
     4741 cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc gtttatcccg cccgttgttg
     4801 atggcaaatt gtggcaggat tatccgacat cagtgttagc cagcgaccgc cgttttttca
     4861 gttttgagcc gggggcgaag tggcacggct ttgaaggata tgccgcggat cagtattttg
     4921 ttgatccgtg caagctgtta ctcactacac caggtatcaa tgccgaaacc ggcgaatata
     4981 gcgactttgg cgttccggcg acgattctgg cgcactatct gcgtgagaac ggcattgtgc
     5041 cggagaagtg cgatctcaac tccattctgt tcttattaac tccggcggaa agccacgaga
     5101 agctggcaca actggtggcg atgctggcgc aatttgaaca gcatattgag gatgactcgt
     5161 cgctggctga ggtgttgccg agcgtttata acaaatatcc ggtgcgctat cgcgactaca
     5221 ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt cagtttcgac gtcaaagacc
     5281 tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt ggtgatgaac ccccaggatg
     5341 cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg gattcgtgat gccgaagggc
     5401 gaattgcggc agaaggggcg ttgccttatc cacctggcgt gctttgcgtg gtacccgggg
     5461 aagtctgggg tggggcggtt caacgttatt tccttgcgct ggaagaaggc gtgaatttgt
     5521 tgccgggatt ttcgccggag ttgcaaggtg tctatagcga aaccgatgcg gatggcatga
     5581 aacgattgta cggttatgtg ttgaagtaag aataaaaaaa cgggtcacct tctggcgacc
     5641 cgtttttctt tgcgtaatta gtggctaacc gtctgtgtgc ctgtcgggac acgaacgtgt
     5701 ttatatttga acatcgccat gaacgcgaag gccagaacca cggaataacc agcgaaaatc
     5761 aaccatacgg tctgccagtc ggtaatgccg ttttgggtgt acatctcaac aactttaccg
     5821 ctcacgatgc cgccgaggat acagccgaag ccgttagtca tcatcaggaa catcccttgt
     5881 gcactggcgc gaattgccgg gctaacttct ttttcgacaa acaccgaacc agagatgttg
     5941 aagaagtcga atgcgcaacc gtaaacgatc atcgacagta ccagcagtac agtaccgaac
     6001 ggagtcgggt cgccgtaagc aaacagcgca aaacgcagga tccacgccac aatactgatc
     6061 atcattacgt tcttaatacc gtagcggctt aagaagaacg ggatggtcag aatgaacagg
     6121 gtttcagaga tctgcgaaat cgacatgatg attgacgcat gctgcacgat gaagctgctg
     6181 gcaaacatcg gatctttgtc gaagctgtgc aggaaggtat taccgaacat gttggtaatc
     6241 tgcagttccg cgcccagcag cattgagaag atgaagaaga ttgccatacg cttgttttta
     6301 aacagcgcga atgcatcgag gcccagcagg gttgtccagc tctgattcgc ttgctgttta
     6361 gcaaccggaa tatgcggcag agtcagggta aacagaacca gaatggcgga aagtgctgcg
     6421 ccaatataca gctgcatgtg gcttaattcg aagccagaca ggctcaccac ccacattgcc
     6481 atgataaagc cgatggtgcc ccagatacgg attggcggga agtcagtaac gatatccatc
     6541 ccggcatttt gcaggcgata gtaagagatg gtgttgatta acccaagcgt tggcatataa
     6601 gcaaacgagt taatcaatat cacaaggaac atcgcttccg gcgtcgtgac ctgtgccgcc
     6661 atgaacagcg tgatagcgcc aatggtgtgg caaatggcat atacccattt cgcacttaac
     6721 catttgtcgg ccacaatccc cagcagcgca ggcataaata ccgcggcgat acccagtgag
     6781 ctataaactg cgccaataga agcaccgtca aacttcaggg taacaaacat ataggagccg
     6841 agggtcgtca gccaacttcc ccacagacag aactgcagaa aagagaggat tttcagctgc
     6901 agcttaagat tcatgttaat ttcctcacat cgtgatgcgg atgagtgttt gaaaaagcac
     6961 atttgaccgt gcgagatgat gggattctat caacttcgcg gactggtttt ttgttacctg
     7021 tggcaaataa ttgcaaacat tcccaatttg caaaacgaaa ttgtgatgtg gataacattt
     7081 ttgcccctga gcatcgtcag gggcggttaa tggaattatc ggcggcggta ggatttttgc
     7141 gcggtattca ctttataaag ataacggcga gattccgcag agggatggcg ggtcgtcagc
     7201 gtctgataaa catcgcccgg cgtcatggtg ttaataatat tggcagcctg aatcttatca
     7261 ttcgaaaaga ctcgcagcac gctgcctgcg ccgccgttat aggcggtgat gacggcataa
     7321 cgccgcgatg ttgggttatc aattccgccg agataaacat tgttcagcat cgccagatac
     7381 gcggtgccgg tatcaatatt gctggcagga tcaaacaaga aactgcggct cggcgtgccg
     7441 gatttcccct gcgagcggaa cacatctttc ccggcagtat tttgtaccac ctgcattaat
     7501 cccagcgcat cggaacggct gaccgcatac gggttaaagg aagattcagt ctgcataatt
     7561 gccagaatca gcgactcatc aacgccatat ttccgtgacg cctggcggac catgccgaga
     7621 tatttgtgcg cacgtttatc aaggtggttc ggcaccatgt taatggtgac gctgtagatg
     7681 atacgtagcc cgttgctgcg gcttttcaga cggtttttca gcagataatc cgcgaagttg
     7741 cttgcgcgac cttcccagcg aatcggctgc ccggtgttgt ccaccacctg accgtaaagg
     7801 aaaggttctt tcgaaattgt aatatcatca acgtcggaat agagatcgac cgaactcgga
     7861 tcgtcaccca tcagcaacgt tttgataatt gcccggcgca aatgcgcggc aggttctgtc
     7921 ccggcgatgg tttcgatagt aatcgtaccg tcatcgaagt tgatgtggct gcgggtctga
     7981 tattgatcgg tgtatttcac gtagtcctta ggaccggcga tcaccacctc tttgaagccc
     8041 cagatgttct caatattgtg ggcaaattgc cccatcagaa tatcaaaacc gttggtatct
     8101 ttgacccagg cttcgttata ggtatcgcct tttttggtcg tcgaacagga gatgagcaac
     8161 ggcgcaatca aagccagcgc gagatatttt ttcatcattc cgggtgcgtg ttgtgtttgt
     8221 ttttatgcaa gctgccggag gcgtgctccg gcactgtttt tattttttat cttccggcgt
     8281 atagccctcg atatgcacct ctttaccctc gaacaggaag ttgaccatct cctgctccag
     8341 cagcttgcgg tgctcggcat tcatcatgtt gagtttcttt tcattaatca gcatggtttg
     8401 tttgtgctgc cactgcgccc aggcttcttt ggagatctcg ttatagatgc gttttcccag
     8461 ctcgccgggg tacagctgaa aatcctgacc ttctgcttca cgttgcagga aagtacaaaa
     8521 aatcgttctg ctcataaatc atcctcttta tcgactcacg cgctaaaccg gcgcgccagt
     8581 gcgtaactgc tgtaacaaac gctccacggg agccgccagg ccaactgacg gcggttgcgc
     8641 taagttatac cagagcgcat tgccttcatc catgcagccg gtgaatgacg acacgggaag
     8701 ccacataggc acaatatcta agtggaaatg gctgaaggta tggcgaaacg cggtcagttg
     8761 cgtcaggtta tcggcagcaa tctgccgttg tgccagccaa tgccgcaaac tttcttcgtc
     8821 ggcaaactgc gggaaacagt ataaaccgcc ccacaatccg ctcggcggac gctgcgccag
     8881 caatacttca tcttcgtgct gtaacaggag aaagtagccg gtgcgctccg gcagcgtctg
     8941 tttcggtttt ttgcccggat aaagcgacca gctattgttg gtggcggcaa tacatccgtt
     9001 ttgtagcgga cagagcgaac atttcggctt cgaacgcgtg caaatcatcg cgcccaaatc
     9061 catcatcgcc tgattaaacc gttccacgcc gaccgcgggc gtcacctgct cgctcaaact
     9121 ccatagttta ttctcgacct cttttttccc aggccagccg cttacagcat agcagcgcgc
     9181 cagcacgcgt ttgacgttac cgtcgagaat cggaaagtgc ttacccagag aaagcgagag
     9241 aatcgcgcct gcggtggaac gcccgacgcc cggtaacgcc gcgacttctt caaaggtttc
     9301 cgggaattta ccaccgtgta aggtcgccac ttgttgtgcc gctttatgca ggttgcgcgc
     9361 gcgggcgtaa tagccaagcc cggtccacaa gtggagaact tcgtcgagcg gcgcattggc
     9421 gagatcggtc accgtcggga agtgcgccat aaagcgttca aaatagggga taacggtcgc
     9481 aacctgagtt tgttgcaaca tcacttctga gagccatact ttgtagggcg tcttgtcaat
     9541 ttgccagggc agcgttttcc gcccgtattt atcgtaccag tccagaacct gggctgaaaa
     9601 ttgcgacgct tgcatggtca ccgacttcac tgttgttggg ggcaagattg cagcacagcg
     9661 acggcagggt gtaaaccgga actttccgca gccacggcct tatcatgctt gcatccggca
     9721 actaactttg gataatgccc gttttcagaa cactttcaca agcgactaaa tctttatgaa
     9781 aaacgacgtc atttcaccgg aatttgatga aaacggccgc ccactgcgcc gtatccgtag
     9841 ttttgtgcgc cgccaggggc gactgaccaa aggccaggaa catgcgctgg aaaactactg
     9901 gccggtgatg ggcgttgagt tcagtgaaga tatgctggat ttccccgcgc tttttggccg
     9961 tgaagcgccg gtgacgcttg agattggttt tggcatgggg gcgtcgctgg tggcaatggc
    10021 taaagatcgc cccgagcagg acttcctcgg cattgaagtg cattcaccgg gcgttggtgc
    10081 gtgcctggct tctgcgcatg aagaaggttt aagcaacctg cgcgtgatgt gtcacgatgc
    10141 ggttgaagtg ctgcataaaa tgattcctga caattcattg cgcatggtgc agctcttttt
    10201 ccctgacccg tggcacaaag cgcgccataa taaacgccgt atcgttcagg tgccgtttgc
    10261 cgaactggta aaaagcaaac tgcagctggg gggcgtattc catatggcga ccgactggga
    10321 accttatgcg gaacatatgc ttgaagtgat gtcttctatt gacggttata aaaacctgtc
    10381 agagagcaat gattacgtac cgcgtccggc atcacgtccg gtgacgaaat ttgaacaacg
    10441 tggtcatcgt cttggtcacg gagtatggga cttaatgttc gagagggtga aataatggca
    10501 aagaaccgta gccgtcgtct gcgtaaaaaa atgcacatcg acgaattcca ggaattagga
    10561 ttttcggtgg catggcgatt cccggaaggt acatcggaag aacagattga taaaatcgtt
    10621 gatgacttta ttaacgaggt tatcgaaccg aacaaactgg cctttgacgg cagcggttat
    10681 ctggcctggg aaggtctgat ctgcatgcag gaaatcggca aatgcaccga agaacatcag
    10741 gcgattgtgc gtaagtggct ggaagagtgc aaactggatg aggtacgcac cagcgaactt
    10801 ttcgacgttt ggtgggacta agaaagcata cgggcgatga caaatgcaaa actgcctgat
    10861 gcgctacgct tatcaggcct acagagatgc acgatcgagt aggcgggata aggcgtttac
    10921 gccgcatccg gcatggaaaa cgcgtacttt gttatcaatc tggggccagc atatgctggc
    10981 ctgatttgtt cttgagggaa gactatgatg cgcaaaatgc tgctggcggc agcactttca
    11041 gtgacggcaa tgaccgctca cgccgactac cagtgcagcg tcacgccgcg tgacgatgtg
    11101 attgtcagcc cgcaaaccgt gcaggtgaag ggcgaaaacg gcaatctggt gatcacgcca
    11161 gacggcaacg tgatgtataa cggtaagcaa tattccctga atgccgccca gcgcgagcag
    11221 gcgaaggatt atcaggctga actacgtagc acgctgccgt ggattgatga aggcgcgaaa
    11281 agccgcgtcg agaaagcccg tattgcgctg gataaaatta tcgttcagga gatgggcgac
    11341 agcagcaaaa tgcgcagccg tctgaccaaa cttgatgcgc agcttaaaga gcagatgaat
    11401 cgcattatcg aaacgcgcag cgatggcctg acgttccact ataaagccat tgatcaggtt
    11461 cgcgccgaag gccagcaact agtgaatcag gcaatgggcg gaattttaca ggacagcatt
    11521 aatgaaatgg gcgcgaaagc ggtgctgaaa agcggcggta acccattaca gaatgtgctg
    11581 ggaagcttgg gcggtctgca atcctcaatc caaaccgagt ggaaaaagca ggaaaaagat
    11641 ttccagcagt ttggcaaaga tgtttgtagc cgcgttgtga ctctggaaga tagccgcaaa
    11701 gccctggtcg ggaatttaaa ataatcctct attttaagac ggcataatac ttttttatgc
    11761 cgtttaattc ttcgttttgt tacctgcctc taactttgta gatctccaaa atatattcac
    11821 gttgtaaatt gtttaacgtc aaatttccca tgcaaagcta agggataatg cgtagcgtcc
    11881 acgtaactgg aggaatgaaa tggagttttt caaaaagacg gcacttgccg cactggttat
    11941 gggttttagt ggtgcagcat tggcattacc caatatcacc attttagcaa ccggcgggac
    12001 cattgccggt ggtggtgact ccgcaaccaa atctaactac acagcgggta aagttggcgt
    12061 agaaaatctg gttaatgcgg tgccgcaact aaaagacatt gcgaacgtta aaggcgagca
    12121 ggtagtgaat atcggctccc aggacatgaa cgataatgtc tggctgacac tggcgaaaaa
    12181 aattaacgcc gactgcgata aaaccgacgg cttcgtcatt acccacggta ccgacacgat
    12241 ggaagaaact gcttacttcc tcgacctgac ggtgaaatgc gacaaaccgg tggtgatggt
    12301 cggcgcaatg cgcccgtcca cgtccatgag cgcagacggt ccattcaacc tgtataacgc
    12361 ggtagtgacc gcagctgata aagcctccgc taatcgtggc gtgctggtgg tgatgaacga
    12421 caccgtactg gacggtcgcg atgtcaccaa aaccaacacc accgacgtag cgaccttcaa
    12481 gtctgttaac tacggtcctc tgggatacat tcacaacggt aagattgatt accaacgtac
    12541 cccggcacgt aagcacacca gcgatacgcc attcgatgtc tctaagctga atgagctgcc
    12601 gaaagtcggc atcgtttata actacgctaa cgcatccgat cttccggcta aagcactggt
    12661 agatgcgggc tatgatggca tcgttagcgc tggtgtgggt aatggtaacc tgtataaatc
    12721 cgtgttcgac accctggcaa ccgccgcgaa aaacggcact gcagtagtgc gttcttcccg
    12781 cgtaccgacg ggtgctacca ctcaggatgc tgaagtggat gatgcgaaat acggcttcgt
    12841 cgcctctggc acgctgaacc cgcaaaaagc gcgcgtcctg ctgcagctgg ctctgacgca
    12901 aaccaaagat ccgcagcaga tccagcagat tttcaatcag tactaatcac ctcgtcccgg
    12961 catcgtgccg gggctttttc acttcagact tacgtccatt gccaattttc attaccctaa
    13021 tgataatcac cggaataaat tattccgcgc gagggttttc gggtgaaaaa gcaatggatt
    13081 gttggcacgg cgctgcttat gttgatgact ggtaatgcct gggcagatgg cgaaccgcca
    13141 actgaaaata tcttaaaaga tcaattcaaa aagcagtatc acggcattct caagcttgat
    13201 gccatcagct taaaaaatct tgatgctaag ggtaatcagg ccacctggtc agcggaaggc
    13261 gatgtctctt ccagtgacga tctctatacc tgggtcggtc agttggcaga ttacgaactg
    13321 ctcgaacaga cctggacgaa agataaaccg gtaaaattct cggcgatgtt aaccagtaaa
    13381 ggaacgcccg cgtctggctg gtcggtgaac ttttactctt ttcaggcggc agccagcgat
    13441 cgtgggcggg tggtcgacga tatcaaaacg aataataaat atctgatcgt gaatagcgaa
    13501 gatttcaatt atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt
    13561 cctgcgctgg aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca
    13621 gcggatgcat attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt
    13681 aaaaaaattc accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg
    13741 gttaaatatg acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa
    13801 gagtgttatg aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga
    13861 cagacttttc tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc
    13921 gaaaaagggc aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg
    13981 attttgatga aaattgacga tatcaaccaa gctaatgaac gctagaaaaa agataccgaa
    14041 caactgcggc gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat
    14101 aaaaagttat ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg
    14161 atatttgcgg aatgtaagtg atcatcatca acgctactag caatgcagca taaaatagca
    14221 gcaatggttt gattaaatgt tgaatcttca cacccgagat agaacaacca acaaatagcg
    14281 cactacccac cggcggtgtt aacagcccaa tacaaaggtt ggccaccatc ataataccga
    14341 aatgtactgg gtccattccc agctcttgtg caatcggcaa gaagatcggt gtaaagatta
    14401 gtacagccgg tgtcatgtcc ataaagatgc cgacaattaa taagacgata ttaatcagca
    14461 gcaatatgat cagtgggtta tcagaaatcc ccatcaatgc gtcgctgatc atataaggaa
    14521 tatcagcatt agtcattgcc caggacatgc ccacggaaaa accgatcaac agtaatacaa
    14581 tcgaagtggt aacaaccgac tccagaatca gtttgggtaa atggcgccat tttacttccc
    14641 ggtaaatcag caccgagaga ataaatgtgt agacaacggc aattgctgac gcttcagttg
    14701 cggtaaaaat gccgcccaga atgccgccca tcacaatgaa caccagaagc aaacttggta
    14761 aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa agcgggtagc
    14821 cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt aagatccctg
    14881 gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc agggaaaaca
    14941 caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag acgtttacag
    15001 cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt gtgccaccaa
    15061 cggcagcagc tgccgcaacg gctgaaccag aaatggaacc aaacatcata ttcgccagga
    15121 cgttcacatg accaagcgaa ccaggaacgc ggccaaccat tacctgggcg aggttaatca
    15181 aacgtatcgc aataccgccg ctgttcatca gtgttccggc aaagatgaa
//
LOCUS       DAJTRN010000091        15065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRN010000091 DAJTRN010000000
VERSION     DAJTRN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO328_003058"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO328_003058"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5931536.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO328_003059"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO328_003059"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5931537.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO328_003060"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO328_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5931538.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO328_003061"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO328_003061"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5931539.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO328_003062"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO328_003062"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5931540.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO328_003063"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO328_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5931541.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO328_003064"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO328_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5931542.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO328_003065"
     CDS             complement(8065..9207)
                     /locus_tag="OO328_003065"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5931543.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO328_003066"
     CDS             complement(9216..10229)
                     /locus_tag="OO328_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5931544.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO328_003067"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO328_003067"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5931545.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO328_003068"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO328_003068"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5931546.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO328_003069"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO328_003069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5931547.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO328_003070"
     CDS             complement(12827..13951)
                     /locus_tag="OO328_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5931548.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO328_003071"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO328_003071"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5931549.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4284 a         3384 c         3101 g         4296 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg aaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgg cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaatatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccg
//
LOCUS       DAJTRN010000092        14551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRN010000092 DAJTRN010000000
VERSION     DAJTRN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..917
                     /gene="thrC"
                     /locus_tag="OO328_003072"
     CDS             <1..917
                     /gene="thrC"
                     /locus_tag="OO328_003072"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5931550.1"
                     /translation="KPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRGKISPLQEK
                     LFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISRLLAQICYY
                     FEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVNDTVPRFLH
                     DGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDETTQQTMRE
                     LKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGETLDLPKEL
                     AERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            1131..1427
                     /locus_tag="OO328_003073"
     CDS             1131..1427
                     /locus_tag="OO328_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5931551.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1580..2356)
                     /gene="yaaA"
                     /locus_tag="OO328_003074"
     CDS             complement(1580..2356)
                     /gene="yaaA"
                     /locus_tag="OO328_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5931552.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(2426..3856)
                     /locus_tag="OO328_003075"
                     /pseudo
     CDS             complement(2426..3856)
                     /locus_tag="OO328_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            4135..5088
                     /gene="tal"
                     /locus_tag="OO328_003076"
     CDS             4135..5088
                     /gene="tal"
                     /locus_tag="OO328_003076"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5931553.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            5203..5790
                     /gene="mog"
                     /locus_tag="OO328_003077"
     CDS             5203..5790
                     /gene="mog"
                     /locus_tag="OO328_003077"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5931554.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(5825..6391)
                     /gene="satP"
                     /locus_tag="OO328_003078"
     CDS             complement(5825..6391)
                     /gene="satP"
                     /locus_tag="OO328_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5931555.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6540..7253)
                     /gene="msyB"
                     /locus_tag="OO328_003079"
     CDS             complement(6540..7253)
                     /gene="msyB"
                     /locus_tag="OO328_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5931556.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(7279..7683)
                     /locus_tag="OO328_003080"
     CDS             complement(7279..7683)
                     /locus_tag="OO328_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5931557.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            8060..9976
                     /gene="dnaK"
                     /locus_tag="OO328_003081"
     CDS             8060..9976
                     /gene="dnaK"
                     /locus_tag="OO328_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5931558.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            10065..11195
                     /gene="dnaJ"
                     /locus_tag="OO328_003082"
     CDS             10065..11195
                     /gene="dnaJ"
                     /locus_tag="OO328_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5931559.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(11299..11451)
                     /gene="mokC"
                     /locus_tag="OO328_003083"
     CDS             complement(11299..11451)
                     /gene="mokC"
                     /locus_tag="OO328_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5931560.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            12037..13203
                     /gene="nhaA"
                     /locus_tag="OO328_003084"
     CDS             12037..13203
                     /gene="nhaA"
                     /locus_tag="OO328_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5931561.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            13269..14168
                     /gene="nhaR"
                     /locus_tag="OO328_003085"
     CDS             13269..14168
                     /gene="nhaR"
                     /locus_tag="OO328_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5931562.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(14206..>14551)
                     /locus_tag="OO328_003086"
     CDS             complement(14206..>14551)
                     /locus_tag="OO328_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5931563.1"
                     /translation="HHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELE
                     NSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQ
                     IMSRVKIVVMYN"
BASE COUNT         3730 a         3554 c         3759 g         3508 t
ORIGIN      
        1 ataagccagt gaccattctg accgcgacct ccggtgatac cggagctgca gtggctcatg
       61 ctttctacgg tttgccgaat gtgaaagtgg ttatcctcta tccacgaggc aaaatcagtc
      121 cactgcaaga aaaactgttc tgtacgttgg gcggcaatat cgaaactgtt gccatcgacg
      181 gcgatttcga tgcctgtcag gcgctggtga agcaggcgtt tgatgatgaa gaactgaaag
      241 tggcgctggg gttaaactcg gctaactcga ttaacatcag ccgtttgctg gcgcagattt
      301 gctactactt tgaagctgtt gcgcagctgc cgcaggaggc gcgcaaccag ctggttgtct
      361 cggtgccaag cggaaacttc ggcgatttga cggcgggtct gctggcgaag tcattcggtc
      421 tgccggtgaa acgttttatt gctgcgacca acgtgaacga taccgtgcca cgtttcctgc
      481 acgacggtca gtggtcaccc aaagcgactc aggcgacgtt atccaacgcg atggacgtga
      541 gtcagccgaa caactggccg cgtgtggaag agttgttccg ccgcaaaatc tggcaactga
      601 aagagctggg ttatgcagcc gtggatgatg aaaccacgca acagacaatg cgtgagttaa
      661 aagaactggg ctacacctcg gagccgcacg ctgccgtagc gtatcgtgcg ctgcgtgacc
      721 agttgaatcc aggcgaatat ggcttgttcc tcggcaccgc gcatccggcg aaatttaaag
      781 agagcgtgga agcgattctc ggtgaaacgt tggatctgcc aaaagagctg gcagaacgtg
      841 ctgatttacc cttgctttca cataatctgc ccgccgattt ttctgcgttg cgtaaattga
      901 tgatgaatca tcagtaaaat ctattcatta tctcaatcag gccgggtttg cttttatgca
      961 gcccggcttt tttatgaaga aattatggag aaaaacgaca gggaaaaagg agaaattatc
     1021 aataaatgcg gtaacttaga gattaggatt gcggagaata acaaccgtcg ttctcatcgc
     1081 gtaatctccg gatatcgacc cataacgggc aatgataaaa ggagtaacct gtgaaaaaga
     1141 tgcaatctat cgtactcgca ctttccctgg ttctggtcgc tcccatggca gcacaggctg
     1201 cggaaattac gttagtcccg tcagtaaaat tacagatagg cgatcgtgat aatcgtggct
     1261 attactggga tggaggtcac tggcgcgacc acggctggtg gaaacaacat tatgaatggc
     1321 gaggcaatcg ctggcaccca cacggaccgc cgccaccgcc gcgccaccat aagaaagctc
     1381 ctcatgatca tcacggcggt catggtccag gcaaacatca ccgctaaatg acaaatgccg
     1441 ggtaacaatc cggcattcag cgcctgatgc gacgctggcg cgtcttatca ggcctacgtt
     1501 aattctgcaa tatattgaat ctgcatgctt ttgtaggccg gataaggcgt tcacgccgca
     1561 tccggcattg actacaaact taacgctgct cgtagcgttt aaacaccagt tcgccattgc
     1621 tggaggaatc ttcatcaaag aagtaacctt cgctattaaa accagtcagt tgctctggtt
     1681 tggtcagccg attttcaata atgaaacgac tcatcagacc gcgtgctttc ttagcgtaga
     1741 agctgatgat cttaaatttg ccgttcttct catcgaggaa caccggcttg ataatctcgg
     1801 cattcaattt cttcggcttc accgatttaa aatactcatc tgacgccaga ttaatcacca
     1861 cattatcgcc ttgtgctgcg agcgcctcgt tcagcttgtt ggtgatgata tctccccaga
     1921 attgatacag atctttccct cgggcattct caagacggat ccccatttcc agacgataag
     1981 gctgcattaa atcgagcggg cgcagtacgc catacaagcc ggaaagcatt cgcaaatgct
     2041 gttgggcaaa atcgaaatcg tcttcgctga aggtttcggc ctgcaagccg gtgtagacat
     2101 cacctttaaa cgccagaatc gcctggcggg cattcgccgg cgtgaaatct ggctgccagt
     2161 catgaaagcg agcggcgttg atacccgcca gtttgtcgct gatgcgcatc agcgtgctaa
     2221 tctgcggagg cgtcagtttc cgcgcttcat ggatcaactg ctgggaattg tctaacagct
     2281 ccggcagcgt atagcgcgtg gtggtcaacg ggctttggta atcaagcgtt ttcgcaggtg
     2341 aaataagaat cagcatatcc agtccttgca ggaaatttat gccgacttta gcaaaaaaag
     2401 agaatgagtt gatcgatagt tgtgattact cctgcgaaac atcatcccac gcgtccggag
     2461 aaagctggcg accgatatcc ggataacgca acggatcaaa caccgggcgc acgccgagtt
     2521 tacgctggcg tagataatca ctggcaatgg tatgaaccac aggcgagagc agtaaaatgg
     2581 cggtcaaatt ggtaatagcc atgcaggcca ttatgatatc tgccagttgc cacatcagcg
     2641 gaaggcttag caaggtgccg ccgatgaccg ttgcgaaggt gcagatccgc aaacaccaga
     2701 tcgctttagg gttgttcagg cgtaaaaaga agagattgtt ttcggcataa atgtagttgg
     2761 caacgatgga gctgaaggca aacagaataa ccacgagggt aacaaattca gcaccctagg
     2821 aacccattag cacccgcatc gccttctgga taagctgaat accttccagc ggcatgtagg
     2881 ttgtgccgtt acccgccagt aatatcagca tggcgcttgc cgtacagatg accagggtgt
     2941 cgataaaaat gccaatcatc tggacaatcc cttgtgctgc cggatgcgga ggccaggatg
     3001 cagctgccgc tgccgcgttt ggcgtcgatc ccattcccac ctcattggaa aacatactgc
     3061 gctgaaaacc gttagtaatc gcctggctta aggtatatcc cgccgcgccg cctgccgctt
     3121 cctgccagcc aaaagcactc tcaaaaatag accaaatgac gtggggaagt tgcccgatat
     3181 ttattacgca aatcaccagg ctggtcagta cccagattat cgccatcaac gggacaaacc
     3241 cctgcatgag ccgggcgacg ccatgaagac cacgagtgat tgccagcaga gcaaagacag
     3301 cgagaataat gcccgtcacc agcggaggaa aatcaaaaga aaaactcagg gctcgggcaa
     3361 cggcgttcgc ctgaactccg ctgaaaatta tgccgtaggc gatgagcaaa aagacggcga
     3421 acagaacgcc catccagcgc atccccagcc cgcgcgccat ataccatgcc ggtccgccac
     3481 gaaactgccc attgacgtca cgttctttat aaagttgtgc aagggaacat tcggcaaacg
     3541 aggtcgccat gccgataaac gcagcaaccc acatccaaaa gacggcacct ggtccaccgg
     3601 cggtaattgc cagcgcaacg ccggccaggt tgccgctacc cacgcgcgcc gcaagactgg
     3661 tacacaatga ctgaaatgag gttaaaccgc ctggctgtgg atgaatgcta tttttaagac
     3721 ttttgccaaa ctggcggatg tagcgaaact gcacaaatcc ggtgcgaaaa gtgaaccaac
     3781 aacctgcgcc gaagagcagg taaatcatta ccgatcccca aaggacgctg ttaataaagg
     3841 agaaaaaatc tggcatgcat atccctctta ttgccggtcg cgatgacttt cctgtgtaaa
     3901 cgttaccaat tgtttaagaa gtatatacgc tacgaggtac ttgataactt ctgcgtagca
     3961 tacatgaggt tttgtataaa aatggtgggc gatatcaacg cagtgtcaga aatccgaaac
     4021 agtctcgcct ggcgataacc gtcttgtcgg cggttgcgct gacgttgcgt cgtgatatca
     4081 tcagggcaga ccggttacat ccccctaaca agctgtttaa agagaaatac tatcatgacg
     4141 gacaaattga cctcccttcg tcagtacacc accgtagtgg ccgacactgg ggacatcgcg
     4201 gcaatgaagc tgtatcaacc gcaggatgcc acaaccaacc cttctctcat tcttaacgca
     4261 gcgcagattc cggaataccg taagttgatt gatgatgctg tcgcctgggc gaaacagcag
     4321 agcaacgatc gcgcgcagca gatcgtggac gcgaccgaca aactggcagt aaatattggt
     4381 ctggaaatcc tgaaactggt tccgggccgt atctcaactg aagttgatgc gcgtctttcc
     4441 tatgacactg aagcgtcaat tgcgaaagca aaacgcctga tcaaactcta caacgatgca
     4501 ggtattagca acgatcgtat tctgatcaaa ctggcttcta cctggcaggg tatccgtgct
     4561 gcagaacagc tggaaaaaga aggtatcaac tgtaacctga ccctgctgtt ctccttcgct
     4621 caggctcgtg cttgtgcgga ggcgggcgtg ttcctgatct cgccgtttgt tggccgtatt
     4681 cttgactggt acaaagcgaa taccgataag aaagagtacg ctccggcaga agatccgggc
     4741 gtggtttctg tatctgaaat ctaccagtac tacaaagagc acggttatga aaccgtggtt
     4801 atgggcgcaa gcttccgtaa catcggcgaa attctggaac tggcaggctg tgaccgtctg
     4861 accatcgcac cggcactgct gaaagagctg gcggagagcg aaggggctat cgaacgtaaa
     4921 ctgtcttaca ccggcgaagt gaaagcgcgt ccggcgcgta tcactgagtc cgagttcctg
     4981 tggcagcaca accaggatcc aatggcagta gataaactgg cggaaggtat ccgtaagttt
     5041 gctattgacc aggaaaaact ggaaaaaatg atcggcgatc tgctgtaatc attcttagcg
     5101 tgaccgggaa gtcggtcacg ctacctcttc tgaagcctgt ctgtcactcc cttcgcagtg
     5161 tatcattctg tttaacgaga ttgtttaaac ggaaaaatct tgatgaatac tttacgtatt
     5221 ggcttagttt ccatctctga tcgcgcatcc agcggcgttt atcaggataa aggcattcca
     5281 gcgctggaag aatggctgac atcggcgcta accacgccgt ttgaactgga aacccgctta
     5341 atccccgatg agcaggcgat catcgagcaa acgttgtgtg agctggtgga tgaaatgagt
     5401 tgccatctgg tgctcaccac gggcggaact ggcccggcgc gtcgtgacgt aacgcccgat
     5461 gcgacgctgg cagtagcgga ccgcgagatg cctggctttg gtgaacagat gcgccagatc
     5521 agcctgcatt ttgtaccaac tgcgatcctt tcgcgtcagg tgggcgtgat tcgcaaacag
     5581 gcgctgatcc ttaacttacc cggtcagccg aagtctatta aagagacgct ggaaggtgtg
     5641 aaggacgctg agggtaacgt tgtggtgcat ggtatttttg ccagcgtacc gtactgcatt
     5701 cagttgctgg aagggccata cgttgaaacg gcaccagaag tggttgctgc attcagaccg
     5761 aagagtgcaa gacgcgacgt tagcgaataa aaaaatcccc ccgagcgggg ggatctcaag
     5821 acaattagtg ggattcacca atcggcagaa cggtgcgacc aaactgctcg ttcagcactt
     5881 cacccatcgc cagatagatt gcgctggcac cgcagatcag accaatccag ccggcaaagt
     5941 ggatgatcgc ggcattaccg gcaatgttac cgatcgccag cagggcaaac agcacggtca
     6001 ggctaaagaa aacgaattgc aggacgcgcg cgcctttcag agtgccgaag aacataaaca
     6061 gcgtaaatac gccccacaga cccaggtaga caccaaggaa ctgtgcattt ggcgcatcgg
     6121 tcagacccag tttcggcatc agcagaatcg caaccagcgt cagccagaaa gaaccgtaag
     6181 aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta ctccagcaga ccagcaaaaa
     6241 tttgcgcgat gccgccgtag aaaatgccca tggcaagaat aataccgtcc agagcgaaat
     6301 aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc gaagcccatc aggcccagcg
     6361 gtgccggatt agccaactta gtgttgccca taattcctca aaaatcatca tcgaatgaat
     6421 ggtgaaataa tttccctgaa taactgtagt gttttcaggg cgcggcataa taatcagcca
     6481 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
     6541 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
     6601 gtcacgcgat aggtgctgcc gctgaccgct ttaaccccat ttaatgctgc gcccacaggg
     6661 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
     6721 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca caccttttgc caataatttg
     6781 tctttcatca gcagcggcag cagctcttcc agctcattca ccctggcatc gaccgcgtgc
     6841 agaaactcct gcttatgttc ctcgtccatt ttcttccagg tattacgcag aaattgttcc
     6901 agtaactgtt gctcaatttc aaacgtagac atctctttgt cggctttcag cttcaatcgc
     6961 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
     7021 atactatcgc caccaaaatg ctgtaattct ccggcaatca gctgccagtt gcggcgatgt
     7081 tgctcgggat gcccttccat cgatttaaac agttcgttgc gcatcagtat gctggagagg
     7141 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
     7201 ctacaatgct gaagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
     7261 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
     7321 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcgct
     7381 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcaggct
     7441 ctgactggcg ctacgggcgg ctttgaaata aaccgatgca ccacttaact gtaaatcgcc
     7501 atggtcggca gagagttgta tgcgtttcac aatgcgacaa acaggaagtt tcagcgccag
     7561 atcgttggtt tcgttacgcg gcattgcaat gacgccgagg agtttatggt cgtttgcctg
     7621 cgccgtgcag cacagcatca ggctaatcgc caggctggcg gaaatcgtaa aaacggattt
     7681 cataaggatt ctcttagtgg gaagaggtag ggggatgaat acccactagt ttactgctga
     7741 taaagagaag attcaggcac gtaatctttt ctttttatta caattttttg atgaacgcct
     7801 tggctgcgat tcattcttta tatgaataaa attgctgtca attttacgtc ttgtcctgcc
     7861 atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt
     7921 gggttacgac cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatgaaattg
     7981 ggcagttgaa accagacgtt tcgcccctat tacagactca caaccacatg atgaccgaat
     8041 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
     8101 ttgtgtagcg attatggatg gcaccattcc tcgcgtgctg gagaacgccg aaggcgatcg
     8161 caccacgcct tctatcattg cctataccca ggatggtgaa actctggttg gtcagccggc
     8221 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
     8281 ccgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
     8341 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
     8401 gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattacc tgggtgaacc
     8461 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
     8521 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
     8581 agctgcgctg gcttacggtc tggacaaagg cactggcaac cgtactatcg cggtttatga
     8641 cctggggggt ggtactttcg atatttctat tatcgaaatc gacgaagttg acggcgaaaa
     8701 aaccttcgaa gttctggcaa ccaacggtga tacccacctg ggtggtgaag acttcgacag
     8761 ccgtctgatc aactatctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
     8821 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
     8881 ttccgctcag cagaccgacg ttaacctgcc atacatcact gcagacgcga ccggtccgaa
     8941 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ctggttgaag atctggtaaa
     9001 ccgttccatt gagccgctga aagttgcact gcaggatgct ggcctgtccg tatctgatat
     9061 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     9121 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     9181 tgctgctgtt cagggtggtg ttctgactgg tgacgtaaaa gacgtactgc tgctggacgt
     9241 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     9301 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     9361 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     9421 gggtcagttc aacctagatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     9481 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     9541 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     9601 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     9661 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     9721 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcgctgactg cactggaaac
     9781 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggaac tggcacaggt
     9841 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     9901 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     9961 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
    10021 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
    10081 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaga aaggcctaca
    10141 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga
    10201 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
    10261 acgatcagta tggtcatgct gcgtttgagc aaggaggcat gggcggcggt ggttttggcg
    10321 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
    10381 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
    10441 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
    10501 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
    10561 gttctggtca ggtgcagatg cgccagggat tcttcgctgt acagcagacc tgtccacact
    10621 gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
    10681 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
    10741 tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc accggcaggc gatctgtacg
    10801 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctgtattgcg
    10861 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
    10921 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac cggtaagcta ttccgtatgc
    10981 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg
    11041 tcgtcgaaac accggtaggc ctgaacgaaa agcagaaaca gctgctgcaa gagctgcaag
    11101 aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg ctcaaagagc ttctttgatg
    11161 gtgtgaagaa gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc
    11221 aggcctgggt aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat
    11281 cactccccgc cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
    11341 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc
    11401 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
    11461 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat aggaggcctc
    11521 gggttgatgg taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
    11581 gacagacagc ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttttgtttta
    11641 tcgattctaa tcctgaagac gcctcgcatt tttgtggcgt aattttttaa tgatttaatt
    11701 atttaacttt aatttatctc ttcatcgcaa ttattgacga caagctggat tatttttgaa
    11761 atattggcct aacaagcatc gccgactgac aacaaattaa ttattacttt tcctaattaa
    11821 tccctcagga atcctcacct taagctatga ttatctgggc ttagggtcac tcgtgagcgc
    11881 ttacagccgt caaaaacgca tctcaccgct gatggcgcaa attcttcaat ggctcgtaaa
    11941 aaacgaatta ttcctacact ataatctgat tttaacgatg attcgtgcgg ggtaaaatag
    12001 taaaaacgat ctattcacct gaaagagaaa taaaaagtga aacatctgca tcgattcttt
    12061 agcagtgatg cctcgggagg cattattctt atcattgccg ctatcctggc gatgattatg
    12121 gccaacagcg gcgcaaccag tggatggtat cacgactttc tggagacgcc ggttcagctc
    12181 cgggttggtt cactcgaaat caacaaaaac atgctgttat ggataaatga tgcgctgatg
    12241 gcggtatttt tcctgttagt cggtctggaa gttaaacgtg aactgatgca aggatcgcta
    12301 gccagcttac gccaggccgc atttccagtt atcgccgcta ttggtgggat gattgtgccg
    12361 gcattactct atctggcttt taactatgcc gatccgatta cccgcgaagg gtgggcgatc
    12421 ccggcggcta ctgacattgc ttttgcactt ggtgtactgg cgctgttggg aagtcgtgtt
    12481 ccgttagtgc tgaagatctt tttgatggct ctggctatta tcgacgatct tggggccatc
    12541 attatcatcg cattgttcta cactaatgac ttatcgatgg cctcccttgg cgtcgcggct
    12601 gtagcaattg cggtactcgc ggtattgaat ctgtgtggtg tacgccgcac gggcgtctat
    12661 attcttgttg gcgtggtgtt gtggactgcg gtgttgaaat cgggggttca cgcaactctg
    12721 gcgggggtaa ttgtcggctt ctttattcct ttgaaagaga agcatgggcg ttctccagcg
    12781 aagcgactgg agcatgtgtt gcacccgtgg gtggcgtatc tgattttgcc gctgtttgca
    12841 tttgctaatg ctggcgtttc actgcaaggc gtcacgctgg atggcttgac ctccattctg
    12901 ccattgggga tcatcgctgg cttgctgatt ggcaaaccgc tggggattag tctgttctgc
    12961 tggttggcgc tgcgtttgaa actggcgcat ctgcctgagg gaacgactta tcagcaaatt
    13021 atggtggtgg ggatcctgtg cggtatcggt tttactatgt ctatctttat tgccagcctg
    13081 gcctttggta gcgtagatcc agaactgatt aactgggcga aactcggtat cctggtcggt
    13141 tctatctctt cggcggtaat tggatacagc tggttacgcg ttcgtttgcg tccatcagtt
    13201 tgacaggacg gtttaccggg gagccataaa cggctccctt ttcattgtta tcagggagag
    13261 aaatgagcat gtctcatatc aattacaacc acttgtatta cttctggcat gtctataaag
    13321 aaggttccgt ggttggcgca gcggaggcgc tttatttaac tccacaaacc attaccggac
    13381 agattcgagc gctggaagag cgcctgcaag gcaaattatt taaacgcaag ggacgtggtc
    13441 tcgaacccag cgagctggga gaactggtct atcgctatgc cgataaaatg ttcaccttaa
    13501 gccaggaaat gctggatatt gtgaactatc gcaaagaatc caatttattg tttgacgttg
    13561 gcgtggctga tgcactttcc aaacgcctgg tcagtagcgt acttaacgcc gcagtggtag
    13621 aaggcgagcc cattcatctt cgctgcttcg aatccaccca cgaaatgctg ctggagcaat
    13681 taagtcagca taaactggat atgatcattt ctgactgtcc gatagactct acgcagcagg
    13741 aaggcctgtt ctccgtgaga attggcgaat gtggcgtgag tttctggtgt acaaatccac
    13801 caccagaaaa accgttcccg gcttgtctgg aagaacggcg acttttgatt cctgggcgac
    13861 gttcaatgtt agggcgcaaa ttgcttaact ggtttaactc ccagggatta aacgtagaaa
    13921 tcctcggcga gtttgatgat gccgctttga tgaaagcttt tggtgcgatg cacaatgcaa
    13981 tcttcgttgc cccaacgctt tatgcatatg acttttatgc cgataaaact gtcgtagaaa
    14041 ttggtcgcgt cgagaatgtg atggaagagt accatgctat ttttgctgag cggatgattc
    14101 agcacccggc ggtacagcga atctgcaata cggattattc tgcgcttttt agtccagcgg
    14161 tgcgttaatc ggcagctccc ccaaagttaa ggtgggggag atagattagt tgtacattac
    14221 cacgattttg actcggctca ttatttgccc gcttgagaca ttgtttccat atgtacgcgg
    14281 gcgaataaat agaggaatct gattacttcc ttcatgggga tgctgaaaag agtagtaatt
    14341 gcttttacca tactggattt cattcgtacc atcattggag agttgaaagc ctatttgacc
    14401 cgcaccgtcc tcactgtttt ctaactcatt ttttaatatg ctggttcctg actcctgtat
    14461 cgtcactggt tcaaagtaat attcaacgtc actgggttca aaaactaatt caccttgctc
    14521 gccctggatg cagtcaaatc ttatatgatg a
//
LOCUS       DAJTRN010000093        14481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRN010000093 DAJTRN010000000
VERSION     DAJTRN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(103..369)
                     /locus_tag="OO328_003087"
     CDS             complement(103..369)
                     /locus_tag="OO328_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5931564.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            643..4167
                     /gene="nifJ"
                     /locus_tag="OO328_003088"
     CDS             643..4167
                     /gene="nifJ"
                     /locus_tag="OO328_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5931565.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4534..5663
                     /gene="ompN"
                     /locus_tag="OO328_003089"
                     /pseudo
     CDS             4534..5663
                     /gene="ompN"
                     /locus_tag="OO328_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5804..6238
                     /gene="uspF"
                     /locus_tag="OO328_003090"
     CDS             5804..6238
                     /gene="uspF"
                     /locus_tag="OO328_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5931566.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6414..7349
                     /gene="ttcA"
                     /locus_tag="OO328_003091"
     CDS             6414..7349
                     /gene="ttcA"
                     /locus_tag="OO328_003091"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5931567.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7478..8845)
                     /gene="dbpA"
                     /locus_tag="OO328_003092"
     CDS             complement(7478..8845)
                     /gene="dbpA"
                     /locus_tag="OO328_003092"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5931568.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9323..10306)
                     /gene="zntB"
                     /locus_tag="OO328_003093"
     CDS             complement(9323..10306)
                     /gene="zntB"
                     /locus_tag="OO328_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5931569.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10561..11793
                     /gene="dgcM"
                     /locus_tag="OO328_003094"
                     /pseudo
     CDS             10561..11793
                     /gene="dgcM"
                     /locus_tag="OO328_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11814..12377)
                     /gene="smrA"
                     /locus_tag="OO328_003095"
                     /pseudo
     CDS             complement(11814..12377)
                     /gene="smrA"
                     /locus_tag="OO328_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12631..14097)
                     /locus_tag="OO328_003096"
     CDS             complement(12631..14097)
                     /locus_tag="OO328_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5931570.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGIACGLAWNIQI
                     MILFRALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT
                     DNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYT
                     LEEGARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFS
                     FSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRW
                     LLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKER
                     LKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSAL
                     FFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="OO328_003097"
     CDS             complement(14094..>14481)
                     /locus_tag="OO328_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5931571.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3859 a         3653 c         3418 g         3551 t
ORIGIN      
        1 cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa cgctggcgac
       61 cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc tgccacagct
      121 cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac acatcccaat
      181 cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg cacaatttgc
      241 ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg cagtcgcctg
      301 ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta cccaaaacgc
      361 tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata aataataggc
      421 agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt acttttttat
      481 ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg ggggacacaa
      541 aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact gggcgcgcag
      601 caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac tattgacggt
      661 aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat ctaccctatt
      721 acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg cttgaagaac
      781 gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc tatcgctacc
      841 gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc gcagggtttg
      901 ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt tgtcctgcat
      961 gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca ttccgacgtt
     1021 atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca ggaagcgcaa
     1081 gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt tattcatttc
     1141 tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc cgatgacact
     1201 attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc gctcaacccg
     1261 gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca gtctcgcgaa
     1321 gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat gaatgatttc
     1381 gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc gcaagcggaa
     1441 cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt tgatgaattg
     1501 ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc cttctccgcc
     1561 aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact ggacagaacc
     1621 aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc actggcagaa
     1681 gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg ctatggtctt
     1741 tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct caacgcggct
     1801 aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct gtcactgccg
     1861 ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta tggccttggt
     1921 agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa ttccacgccg
     1981 tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct gacggtttct
     2041 caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca ggctgatttt
     2101 gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg tttaaaacct
     2161 agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc gcgcttgccg
     2221 caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat taacgcggcg
     2281 aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca gatggctttt
     2341 ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca gggtgcgatt
     2401 gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca ggctctggcg
     2461 ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca cagcgccaat
     2521 cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac cgcggcgatg
     2581 ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg cacctggccg
     2641 atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat ctggaaagag
     2701 gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc tattcgcgca
     2761 aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc gctagatgtg
     2821 aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga agattgcacc
     2881 ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc agagattaaa
     2941 gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa ttacgatttc
     3001 ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat tcgtacatcg
     3061 cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg cgagacgccg
     3121 tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa cgccaccggc
     3181 tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga tgccaacggt
     3241 cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg ccttggtttc
     3301 cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca atttgccgat
     3361 aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga ggttcgtcga
     3421 gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca tgaacttctg
     3481 cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga cggctgggct
     3541 tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa cgtcaacatt
     3601 ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa agcgacaccg
     3661 ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa agatcttggc
     3721 gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg cgcgcagctg
     3781 aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc gctgatcatt
     3841 gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga ccagatgcgc
     3901 caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg tgccgatgaa
     3961 ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga agaaacgtta
     4021 cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc agaacagtta
     4081 tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca aatggccgga
     4141 aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca attctaaaaa
     4201 aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca gtggcaatgc
     4261 aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc gcagaataac
     4321 gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat acattcacca
     4381 gacttattat catttcagag gaattatttg attaaggttt tacttaaggc gtaacaaatg
     4441 atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga aatggggata
     4501 aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact ggcactttta
     4561 attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa taaagacggc
     4621 aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga taattcagcg
     4681 aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat
     4741 caacttactg gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca
     4801 aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga ttacggttct
     4861 ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac cgatatgctg
     4921 cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg tcgagccaat
     4981 ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct gaacttcgcg
     5041 gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac caataacgaa
     5101 cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata tgatttaggc
     5161 atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga ccaggttaac
     5221 catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa atacgatgct
     5281 aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc gtttggcgac
     5341 agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac cagtttgatt
     5401 tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt cacgctgctg
     5461 gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac gccgatgttg
     5521 gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa atcaacctgt
     5581 tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat attgtcgctt
     5641 taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc tttttctgct
     5701 tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac aaaaagtgtc
     5761 atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca gaacgattct
     5821 tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc acgttgaggc
     5881 agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt cactgcccta
     5941 ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg acctgaaagc
     6001 ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa ccgacagagt
     6061 gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg cgaagaagat
     6121 ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt atctgctcgg
     6181 ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg tgcgctgaca
     6241 ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc tgacattttc
     6301 ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat gctgagcgtt
     6361 ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt taaatgtcgc
     6421 aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa aaacgtctgc
     6481 gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc gatcgcatca
     6541 tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg cgcaatttgc
     6601 agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa aagcaaccgg
     6661 gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag tacaagattg
     6721 ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa accacttgct
     6781 cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa ctggggacga
     6841 cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc ttaaatatgt
     6901 tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat ggcaaacata
     6961 tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt gccgatgcaa
     7021 aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg caacgtcagg
     7081 tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc gagacgatgt
     7141 tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg ttcgatttca
     7201 aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt gatcgcgaag
     7261 agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag ttggatgagt
     7321 tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc aagcgtatcg
     7381 ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca atcccgaata
     7441 agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac cgcacccggc
     7501 acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga gcaacagcct
     7561 gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata tctgcgccat
     7621 caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt ttggcttttt
     7681 tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg gaactattag
     7741 ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata ttggcccgct
     7801 gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca cgagctgtac
     7861 gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc accaccagct
     7921 caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt acacgggcgc
     7981 taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg tgtaacgaca
     8041 atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct tttttggtat
     8101 tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc aacagaggaa
     8161 ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa gcatctgttg
     8221 agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg gcgatggctt
     8281 ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat
     8341 catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc atcaccaaag
     8401 tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa cgacccggcg
     8461 ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg aacggttgac
     8521 caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga cgcaattcac
     8581 ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg gtttgaaata
     8641 gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg ccgctgccgg
     8701 ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg gcggcctgca
     8761 ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg ggaggcaaaa
     8821 cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc aaagttagca
     8881 ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt cttcatcggg
     8941 tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa caggatcgcc
     9001 aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat ttccctccgg
     9061 tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa aaagccgact
     9121 catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa aaggaagtaa
     9181 gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag ggagatattg
     9241 ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt ttgtatacag
     9301 acagccggaa aaattgcttt ttttacaacc atttactacg gtgcaaccat aaagcaacac
     9361 caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc cacccgccgc
     9421 cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg ggtaaaaaga
     9481 ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc atcacctgag
     9541 cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg tcaaggccgc
     9601 gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc atccacggca
     9661 aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag cgacgcatca
     9721 caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc tgatcaagga
     9781 gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca ctggaatgat
     9841 cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga cccgtgccct
     9901 cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa
     9961 ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt tcatcggtgc
    10021 tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg actcggggcc
    10081 gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt gttgtcgcca
    10141 gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga tgcgcttcat
    10201 caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc atccaggcaa
    10261 atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg cctccatcat
    10321 caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc atcaccccat
    10381 taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa aatgcttttc
    10441 cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt atctgatcat
    10501 atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa cgggatctca
    10561 atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg gattgtttcg
    10621 ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat gcaagacctc
    10681 acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga actgccgaaa
    10741 tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt tcagcgtaaa
    10801 gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga aacagaaccg
    10861 gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg
    10921 gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac taactctgca
    10981 ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa cctcgccgcg
    11041 ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta ggaaataaat
    11101 atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc cggcggtcat
    11161 aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt gcagacctat
    11221 gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca tgatattact
    11281 gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc gatgaccgga
    11341 ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca gcatctcgcc
    11401 atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca cattaacgat
    11461 ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc cctcgaaagt
    11521 tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt cttattgcta
    11581 ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt aagcgtggca
    11641 aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc gcatcacgaa
    11701 ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta tcgggcgaaa
    11761 aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg agatcaacga
    11821 ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt acgtagtgcg
    11881 acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc ctgaacatca
    11941 tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg tgatttatct
    12001 tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag ggcttgttga
    12061 atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag attcaggctc
    12121 gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta caacccttcc
    12181 tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt agtgaggaaa
    12241 ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt tggatgccag
    12301 tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag aaacagcgat
    12361 ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt cagacgggtt
    12421 aattgttcat acttactgca tcgttccctt cacagattga aacaatactc tacttcatac
    12481 ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta tcgaacagtt
    12541 tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata gcaggaacca
    12601 acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa atgccatagt
    12661 ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta tttccgatgt
    12721 ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt cattcaccgt
    12781 atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag ttcgattatt
    12841 gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg ttaaattaaa
    12901 tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta atgttacgat
    12961 gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca gtaattcctg
    13021 ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa atccgcccag
    13081 ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt aaaagggaac
    13141 agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt cggcatttaa
    13201 tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata tcccaacccc
    13261 tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag catgaagatc
    13321 cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac tcaccaatgc
    13381 cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg ctccttcttc
    13441 aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg gataatcagc
    13501 aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa tactcaaaac
    13561 taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga ggttatcagt
    13621 gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg cactgactac
    13681 cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg tgaaaactaa
    13741 agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca tgatctgaat
    13801 attccatgca agtccacacg cgattccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DAJTRN010000094        14426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRN010000094 DAJTRN010000000
VERSION     DAJTRN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..132)
                     /locus_tag="OO328_003098"
     CDS             complement(<1..132)
                     /locus_tag="OO328_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5931572.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO328_003099"
     CDS             315..458
                     /locus_tag="OO328_003099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931573.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO328_003100"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO328_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5931574.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO328_003101"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO328_003101"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5931575.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO328_003102"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO328_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5931576.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO328_003103"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO328_003103"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5931577.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO328_003104"
     CDS             complement(7051..7659)
                     /locus_tag="OO328_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5931578.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO328_003105"
     CDS             complement(7730..8815)
                     /locus_tag="OO328_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5931579.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO328_003106"
     CDS             complement(8818..9180)
                     /locus_tag="OO328_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5931580.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO328_003107"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO328_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5931581.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO328_003108"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO328_003108"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5931582.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO328_003109"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO328_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5931583.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO328_003110"
     CDS             complement(13472..13681)
                     /locus_tag="OO328_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931584.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="OO328_003111"
     CDS             13965..14327
                     /locus_tag="OO328_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5931585.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3528 a         3842 c         3624 g         3432 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaacag
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac caaaca
//
LOCUS       DAJTRN010000095        14225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRN010000095 DAJTRN010000000
VERSION     DAJTRN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..718)
                     /gene="fabY"
                     /locus_tag="OO328_003112"
                     /gene_synonym="yiiD"
     CDS             complement(<1..718)
                     /gene="fabY"
                     /locus_tag="OO328_003112"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5931586.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAH"
     gene            complement(763..1200)
                     /gene="dtd"
                     /locus_tag="OO328_003113"
     CDS             complement(763..1200)
                     /gene="dtd"
                     /locus_tag="OO328_003113"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5931587.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1197..2069)
                     /locus_tag="OO328_003114"
     CDS             complement(1197..2069)
                     /locus_tag="OO328_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5931588.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2063..2662)
                     /gene="yihX"
                     /locus_tag="OO328_003115"
     CDS             complement(2063..2662)
                     /gene="yihX"
                     /locus_tag="OO328_003115"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5931589.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(2761..3546)
                     /locus_tag="OO328_003116"
     CDS             complement(2761..3546)
                     /locus_tag="OO328_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5931590.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3580..4476)
                     /gene="yihV"
                     /locus_tag="OO328_003117"
     CDS             complement(3580..4476)
                     /gene="yihV"
                     /locus_tag="OO328_003117"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5931591.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4644..5540
                     /gene="yihU"
                     /locus_tag="OO328_003118"
     CDS             4644..5540
                     /gene="yihU"
                     /locus_tag="OO328_003118"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5931592.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5564..6442
                     /gene="yihT"
                     /locus_tag="OO328_003119"
     CDS             5564..6442
                     /gene="yihT"
                     /locus_tag="OO328_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5931593.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6459..7700
                     /gene="yihS"
                     /locus_tag="OO328_003120"
     CDS             6459..7700
                     /gene="yihS"
                     /locus_tag="OO328_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5931594.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            7838..8740
                     /locus_tag="OO328_003121"
     CDS             7838..8740
                     /locus_tag="OO328_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5931595.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            8939..10975
                     /gene="yihQ"
                     /locus_tag="OO328_003122"
     CDS             8939..10975
                     /gene="yihQ"
                     /locus_tag="OO328_003122"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5931596.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11021..12395
                     /locus_tag="OO328_003123"
                     /pseudo
     CDS             11021..12395
                     /locus_tag="OO328_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12438..13841
                     /locus_tag="OO328_003124"
     CDS             12438..13841
                     /locus_tag="OO328_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5931597.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            13909..>14225
                     /gene="ompL"
                     /locus_tag="OO328_003125"
     CDS             13909..>14225
                     /gene="ompL"
                     /locus_tag="OO328_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5931598.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAV"
BASE COUNT         3446 a         3690 c         3587 g         3502 t
ORIGIN      
        1 tatgcgcatc cgccagaata atcgttccgc cgaggtggcg ttcacgcagc atcagccaga
       61 taagccccca accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt
      121 gcggattctg attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt
      181 gaatacgcac gcccattttt tcactaagcg ggatatgttc gtaccacgcc tgttgcagct
      241 gcgcgcacca gtcgccgcga tgcaaaatat catccagagt ggcgacgggc ttaatcatca
      301 aaaaatggcg aatcggcgtg gtggttggcg tggtgatttc tccctgatta acaaacccca
      361 gcttggcgaa aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt
      421 cctgacgcgc caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca
      481 cgtccgggtg aacgaccata aagcgaatgg acgcttcatt gtcggcatta atatacaatc
      541 ggcctaccgc caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat
      601 cccacgcgtc gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc
      661 gaaagtgata gtaacgctct aactcttctt ctgtttgtgg aacctgaagg tgatacatag
      721 cgatactctc tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa
      781 tgtcacgggg ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg
      841 cgtgttcatc tcttgctggc ggcagcgttc gacgaaatag tcatataacg cctctgcgcg
      901 atccggtgat gcgcctttgg agaaacttgg gcgcatcccc cgttcggtat ctgcggcgag
      961 ggtaaactgg gaaaccacca gtacactgcc gcccgcctgt tgcacgttga gattcatctt
     1021 gccttcggca tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt
     1081 ctgttcgtcg tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc
     1141 cgtcacttct ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat
     1201 ggttcgtcgt cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg
     1261 acagtaattt ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga
     1321 atggggatca ccgccagcac accgtaaatg agctgatatg acgggaacat ggtgatataa
     1381 agcgcgaaac ctttctttcc tgcttcgaac aggagtgcgg cgacaaacgc gccgacaatc
     1441 gcgtcgcggt taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc
     1501 cacgacaaca gcaacggaaa aatacgcagc acgttgtcga tgacagtatt gagatcgctc
     1561 gcccagcgca gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc
     1621 cccagcgtta aaatcatcca gtacacggcg aacgagtaaa tttttggtcg cgctcgttta
     1681 ctgcgccaga tggtattcaa cgcgctatcg atggagtaca tcaacaataa cgcggtgacg
     1741 atcagcccgc acgccccaac ggcggtcatc ttgttggaat tagcaacaaa ttgttcgata
     1801 taccgctgaa taacatcgcc agtagcaggc agaaagttgg caaaaataaa gtgacgtaac
     1861 tgaatgctga cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc
     1921 agcggcacta atgagagcaa cgacacatag gcaaggttac ctgccagggt tgtcatgttg
     1981 tcctcatcaa tacgttgcca gagtagtttt agccaggccc atagtggacg ggtacggtgc
     2041 ctggctttgt cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg
     2101 ttttatcttt caccagaata ctggtaatgc ctagctgatt agctccttct atattatcgg
     2161 cgttatcgtc gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct
     2221 ggtaaattcg tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag
     2281 cagcatcacg aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat
     2341 tggaaagcac caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt
     2401 ccgggcgcag cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg
     2461 gtagagccat ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt tccccacgct
     2521 catgctgatg aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac
     2581 gcgttaaatc gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac
     2641 ctaaatcaaa gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc
     2701 gggaaaggat gggtttggct atgtggcaag gtatgaagcc aggttaacgg tcacaaaaat
     2761 ctacgcgtct tcctggggaa gaatgatttc aatttggctg tcttgtaagc gtgattttag
     2821 cgcagcgggc ggcagctcgt cggtaaagag cgcagtgacc tgtgccacgt taccaatttc
     2881 aaccgccgcc gaagcatgat acttagtgtg atcggcgacc agcagaatat ttctcgcgtg
     2941 cgccatcatc gttttcacca cgttagcttc gtttacatca aactccatca acgcgccatc
     3001 gctctcaatc gccccaacgc ttgttaccag ataatcagca cgaaaatcag ccacaaagga
     3061 cgccgctgaa gggccaatga tcccgctatt atgagagcgc aacgtaccgc cgggcaccat
     3121 cacttcaaag cgcgggttgt ggtaaagaat atgcgccaca cgcaggctgt tggtgattat
     3181 ccgcaaatga ttatggttaa gtaacgcccg ggcaacatgc tcaacagtcg taccaatggt
     3241 gataaatatt gttgaaccat caggaatata gtctgccacc gcttcggcaa tcgctttttt
     3301 tttctcggtt tgcgaaacct cacgctgctc gaacgccgta ttaacgacgc tggaagcccg
     3361 acccgcgcca ccgtgatggc gcgtaatcag gccttgctcg cttaatttac gaatatcccg
     3421 gcggaccgtc tgcgtggaga catccagcag atttgccagc tcatcaatat tcatataccc
     3481 acgctcggcg atcagcatga ggagttggtc gtgccgcggg ttaccggtta gttcggtaag
     3541 gctcatgaaa attcctcgaa aaaccatcac cctggcattt tatacaaaaa gtgacaaaaa
     3601 agatcgggtt tgatcacagt cagggatccc ggctcgtcca ccgggacgtg tgcattttaa
     3661 cgccgctaca ccgctggcga agcggactga ctccgctaaa tccccacttg ttgccagcgc
     3721 caccgccaaa gcgccgtgaa aaacatctcc cgcacctgtg gtatctacca catcaacttt
     3781 gaaggccggt tgatgctgac gcccaccatt ttccagccag tcgcagcctg cgctaccctg
     3841 ggtcacatag acatgaccat ttgtgagcgt ttgtgcctgt tttagcgcac tggccatctc
     3901 tttcacgccc gttaagcgcg ccagacccgg ttctgaaaag gccgcgtgat cgcttaatgc
     3961 caccagctca ctgatatcct gcggcgtaat gtccccgtcc agaacggtca tcacacccgc
     4021 ctgacgggcc agggtgaagg cttttttagc gccgtcgtgc cagcgtacat ctgccagcac
     4081 aacatcccac tgagagaaat caatttcctc caaccactct gcgtcaggca gcagatccgg
     4141 gctggggtag ttaatgatta tccgctcgcc tttggtatcc accatgatgg cggattgcga
     4201 agatttcgcc tggttatacc gtttggtgta acgggtgtta acgccccagg attccagctc
     4261 tgccagcagg ctgttgcccg cgtcgtcatc acctacgcga ccaataaaat cgacctgcgc
     4321 ccccagcctt gccgccgcaa ccgctgccgt cgccgctggc ccgccaccaa cttccgtata
     4381 atttctcgcc acgtatttac cgctctccgt cggtaaccct tccacgtaat agatgcgatc
     4441 catcacggtt atacctacac aagcaacacg aatcatggtc attccttaaa cattttggga
     4501 tgcgttcatt ttaatttcac caattgttta aaaagtgacg gctgtcaatt ttttgacgat
     4561 aaattacaaa ttcgatcaaa aacagacaag caaagcatgt cataaatgac aaaaagtgac
     4621 atcactgtat tcaggagagg gttatggcag caatcgcgtt tatcggttta ggacaaatgg
     4681 gttcgccaat ggcgagcaat ttattgcagc aagggcatca acttcgcgtc tttgatgtga
     4741 acgccgaagc tgtgcggcat ctggtagaca aaggtgcgac tcccgccgcc aacccggcgc
     4801 aggccgctaa agatgccgaa tttatcatta ccatgttgcc gaatggcgat ctggtgcgca
     4861 acgtgttgtt cggtgaaaac ggcgtttgcg aaggcttatc taccgatgcg ctggtcattg
     4921 atatgtccac catccatccg ctgcaaaccg ataaattgat tgccgatatg caagccaaag
     4981 gcttcagcat gatggatgtt ccggtaggcc gtacttctgc aaatgccatt accggtactc
     5041 tgttactgct ggctggcggc accgctgaac aagttgaacg tgccacaccg atcctgatgg
     5101 cgatgggcag tgagttgatc aacgctggcg gtccgggcat ggggatccgc gttaagctca
     5161 tcaacaacta catgagcatc gcgctcaatg cgctttcggc agaagccgcc gttttgtgcg
     5221 aagccctgaa tcttcccttc gatgttgccg tcaaagtgat gagcggtacc gccgccggta
     5281 aaggtcactt caccacttcc tggccaaaca aagtcctcag cggagatctt tctcccgcct
     5341 tcatgatcga tcttgcccat aaggatcttg gcatcgccct tgatgtcgcc aaccagctgc
     5401 atgtgccaat gccgctgggg gccgcctcac gggaggttta tagccaggcg cgcgcagcgg
     5461 gtcgcggtcg ccaggactgg tccgccattc tggaacaggt ccgtgtcagt gccgggatga
     5521 ctgccaaagt aaaaatgtaa cgactggata aaggaataaa tgaatgaata agtacaccat
     5581 caacgacatt acgcgcgcat cgggcggttt tgccatgctg gcggtcgatc agcgcgaagc
     5641 catgcgcatg atgtttgccg cggctggggc acccgctccg gtagccgata gcgttttaac
     5701 tgatttcaaa gttaacgctg caaaggccct ctcgccttat gcctcggcga ttctggtaga
     5761 tcaacaattc tgctaccgcc aggtggttga gcaaaacgcg attgccaaaa gttgcgccat
     5821 gattgtcgcc gccgatgagt tcattcctgg caacggtatt ccggtcgata gcgtggttat
     5881 tgaccgcaaa atcaatccgc tacagatcaa acaagacggt ggcaaagcct taaaactgct
     5941 ggtgctgtgg cgtagtgatg aagatgcgca gcaacgtctg gatatggtga aagagttcaa
     6001 cgaactgtgc cactcacacg gtctggtaag catcattgag ccagtcgtcc gcccaccgcg
     6061 tcgtggcgat aaattcgatc gcgaacaagc gatcatcgat gccgccaaag agctgggcga
     6121 cagtggcgct gacctctaca aagttgaaat gccgctttat ggcaaaggtc cgcaacaaga
     6181 gcttctctgt gcttcacaac gtctgaatga ccatatcaat atgccatggg tgatcctctc
     6241 ttccagtgtc gacgaaaaac tgttcccgcg tgccgtacgc gtggcaatga cggcgggcgc
     6301 atcgggattc ctggcaggcc gtgcagtctg ggcatcggtc gtcggtttac cagacaacga
     6361 gctgatgctc cgtgacgttt gcgcaccgaa attacaacaa cttggcgata tcgtcgacga
     6421 aatgatggct aaacgccgct aagatgagga taaaaggaat gaaatggttt aacaccctaa
     6481 gccacaaccg ttggctggaa caggaaaccg accgcatctt tgattttggt aaaaattccg
     6541 tagtgccgac tggttttggc tggttaggca ataaagggca aatcaaagaa gagatgggca
     6601 cccatctgtg gatcacagct cgtatgttgc acgtttattc cgttgctgcg gcgatgggtc
     6661 gacctggcgc ttacgcgttg gttgatcacg gtattaaagc catgaacggc gcactgcgtg
     6721 ataaaaaata tggcggctgg tatgcctgcg tgaatgacga gggcgtggtg gatgcctcca
     6781 aacagggcta tcaacacttc tttgctctgc tgggtgccgc cagcgccgtc acaacgggtc
     6841 acccggaagc gcgcaagctg ctcgattaca ccattgagat tatcgagaaa tatttctgga
     6901 gcgaagaaga gcagatgtgc ctggagtcct gggacgaagc cttcagcaaa accgaagagt
     6961 accgcggcgg caatgccaat atgcacgcgg tggaagcttt cttgattgtt tatgacgtca
     7021 ctcacgacaa aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt atccacgacg
     7081 tcgccagaaa taatcattat cgcgttaacg aacacttcga tacccagtgg aatccgctgc
     7141 cggattacaa caaagataac ccggcgcatc gcttccgcgc gttcggaggt acaccaggcc
     7201 actggatcga atggggccgt ttaatgctgc acatccatgc tgccctggaa gcccgttgcg
     7261 aacaaccacc agcatggctg ctagaagatg ccaaaggtct gtttaacgcc accgtgcgcg
     7321 atgcctgggc acccgatgga gcggacggga ttgtttatac cgttgactgg gaaggaaaac
     7381 cggtggtccg cgaacgtgta cgttggccta tcgtcgaagc aatgggtacg gtctacgcgc
     7441 tctacaccgt cactggcgat cgccagtatg aaacctggta tcaaacatgg tgggagtact
     7501 gcattaagta cctgatggac tatgaaaatg gttcctggtg gcaggagctg gatgcggaca
     7561 ataaggtcat caccaaagtc tgggacggca aacaggatat ttatcacctg ctgcattgcc
     7621 tggtgatccc gcgtatcccg ttagcgcccg gcatggctcc agcggttgca gcgggtctgc
     7681 tggatattaa tgcgaaataa ctgatctgaa aatagggtgc gggttttccc ctcaccctaa
     7741 ccctctcccc aaaggggcga ggggaccgtc cggtgcagtt tttgggtttg acatcagcct
     7801 cacgggaggc tgtatgtcgt taattaaggt gccttgtatg caaatcacca atatgcattg
     7861 cagtggacag accgtttcac ttgccgcggg ggattatcac gcgaccattg tgaccgtcgg
     7921 tgctggtctg gctgaattaa cctttcaggg ttgtcattta gtcatcccgc acaaacccga
     7981 agagatgccg ctggcgcatc tgggtaaagt gcttattccg tggccgaacc ggatcgccaa
     8041 cggctgttac cgttatcagg ggcaggaata tcagttaccc attaatgaac atagttcaaa
     8101 agccgctatt cacgggctgc ttgcctggcg agactggcaa attagtgaat taaccaccac
     8161 cagcgttacc ctgacagctt ttctgccgcc cagttacggc tatccgttta tgctggcttc
     8221 acaagttgtt tattcgctca acgcacacac gggtctttcc gttgagatcg caagccagaa
     8281 tatcggcact gtcgcagcgc cttatggcgt gggtattcat ccttatttaa cctgcaactt
     8341 aacatctgtt gatgaatatc tgttccagct tccggcaaac caggtttatg ccgtcgatga
     8401 acacgtaaat ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt tcacgcaggc
     8461 gaaaaaaata gccgcgacaa aaatcgacca cacctttaaa accgctaatg atttgtggga
     8521 aatgaccatt acccatccac agcaggcgct ttcggtaagc ctttgttccg atcaaccctg
     8581 ggtgcaggtt tatagcggtg aaaaattaca gcgtcaggga ctggcagttg aacctatgag
     8641 ttgcccgcct aacgccttta attcaggtat cgacttatta ttactggaac caggaaaacc
     8701 gcaccgcctg ttttttaata tttacggaca gagaaaataa cttgtattga ttagcagtca
     8761 aaatcattga agaacatttc actcgctaaa ggcaacatta tgtatcttca atttattaag
     8821 ttaaaaacag ctcagcttct gtaaagtttt tatatatcag ttttatgaga atcattttac
     8881 ataagcctgt aataacagga aggcaacaat ctcgttctca actctatgga gattagttat
     8941 ggatacgcca cgtccacagt tattagattt tcaatttcat cagaataacg acagttttac
     9001 cctacatttt caacaacgtc ttattttaac ccataacaaa gataatcctt gtttatggat
     9061 tggctcaggt atagcggata tcgatatgtt ccgcggtaat ttcagcatta aagataaact
     9121 acaggagaaa attgcgctta ccgacgccat cgtcagccag tcaccggatg gttggttaat
     9181 tcatttcagc cgtggttctg acattagcgc cacgctgaat atctctgccg acgatcaggg
     9241 ccgtttattg ctggaactac aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct
     9301 tgccgctcaa ccagaggacc atatctacgg ctgcggcgaa cagttttcct acttcgatct
     9361 gcgcggcaaa ccgttcccgc tatggaccag tgaacaaggc gttggtcgca acaaacaaac
     9421 ctatgtcacc tggcaggccg actgcaaaga aaatgcgggc ggcgactatt actggacttt
     9481 cttcccacag cctacgtttg tcagcacgca gaagtattac tgccatgttg ataacagttg
     9541 ctacatgaac ttcgacttta gcgccccgga ataccatgaa ctggcgctgt gggaagacaa
     9601 agctacgcta cgttttgaat gtgctgacac atacatctcc ctgctggaaa aattaactgc
     9661 cctgctggga cgccagccag aactgcccga ctggatttat gacggggtaa cgctcggcat
     9721 tcagggcggg acggaagtgt gccagaagaa actggacacc atgcgtaacg cgggcgtgaa
     9781 ggttaacggc atctgggcgc aggactggtc tgggatccgt ataacctctt ttggcaagcg
     9841 cgtgatgtgg aactggaagt ggaacagcga aaactacccg caactggatt cacgcattaa
     9901 gcagtggtat caggagggcg tgcagttcct ggcctatatc aacccgtatg ttgccagcga
     9961 taaagatctc tgcgaagaag cggcacaaca cggctatcta gcaaaagatg cctctggcgg
    10021 tgactatctg gtggagtttg gcgagtttta cggcggcgtt gtcgatctca ctaatccaga
    10081 agcctacgcc tggttcaagg aagtgatcaa aaagaacatg attgaactcg gctgcggcgg
    10141 ctggatggct gacttcggcg agtatctgcc caccgacacg tacctgcata acggcgtcag
    10201 tgccgaaatt atgcataacg cctggcctgc gctgtgggcg aagtgtaact acgaagccct
    10261 tgaagaaacg ggcaagctcg gcgagatcct cttctttatg cgcgccggtt ctaccggtag
    10321 ccagaaatac tccaccatga tgtgggcggg cgaccagaac gtggactgga gtctcgacga
    10381 tggcctggcg tccgtagtcc cggcggcgct gtcgctggca atgaccggac atggtctgca
    10441 tcacagcgac attggcggtt acaccaccct gtttgagatg aagcgcagca aagagctgct
    10501 gctgcgctgg tgcgatttca gcgccttcac gccgatgatg cgcacccacg aaggtaaccg
    10561 tcctggcgac aactggcagt ttgacggcga tgcggaaact atcgcccatt ttgcccgtat
    10621 gactaccgtt ttcaccaccc tgaaacctta tctgaaagag gccgtcgcgc tgaatgcgaa
    10681 gtccggtctg ccggtcatgc gcccgctgtt cctgcattac gaagacgatg cacataccta
    10741 ttccctgaaa tatcagtacc tgttaggccg cgacattctg gtcgctccgg tacacgaaga
    10801 aggtcgcagc gactggacgc tctatctgcc ggaggataac tgggtacatg cctggacggg
    10861 tgaagcgttc cggggcgggg aagttaccgt taatgcgccc atcggcaagc cgccggtctt
    10921 ttatcgcgcc gatagcgaat gggcggcact gttcgcgtcg ttaaacagca tctaatcaca
    10981 tccgcccgtg gggaaactca tgggcaaagg gagaacatcg atgagtcaca tcacaacgga
    11041 agatccagca actttacgcc tgccctttaa agagaaactc tcttacggta ttggcgacct
    11101 ggcctctaac atcctgctgg atatcggtac gctttatctt ttgaagtttt ataccgacgt
    11161 tctggggctg ccaggcacct atggcggcat tatctttttg atttcaaaat tctttactgc
    11221 gtttaccgat atgggtaccg gcattatgtt ggattcccga cgcaagatcg gtccaaaagg
    11281 taagttccgt ccttttattc tgtatgcgtc attcccggtc accttactgg cgatcgccaa
    11341 ctttgtcggc acaccgtttg atgtcaccgg taaaacggtg atggccacta ttctgtttat
    11401 gctttacgga ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg tcccggcaat
    11461 caccaaaaac cccaacgaac gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct
    11521 gggcctgctg ctgtgcacgg tgggattcgt gccagttatg aatcttatcg aaggtaatca
    11581 gaaacttggc tatatcttcg ccgccacgct gttttcactg ttcggcctgc tgtttatgtg
    11641 gatctgctac tcaggcgtga aagagcgtta tgtcgaaacc caaccaacca atccggcgca
    11701 aaagcctggc ctgttgcaat ctttccgcgc aattgccggt aaccgcccac tgttcattct
    11761 gtgtattgcc aacctctgca ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta
    11821 ttacacccag tacgtactta acgatcccat cctgttgtcg tatatgggat ttttcagcat
    11881 gggctgtatt ttcatcggcg tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa
    11941 gaaggtctat atcggcggcc tgctgatttg ggtgctgggc gatctgctca actatttctt
    12001 cggcggcggc tcggtcagct ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgcc
    12061 tgaactgggc gctggtttcc gacaccgtcg agtacggcga gtggcgcacc ggcgtgcgtt
    12121 cggaaggcac ggtctacacc ggcttcacct tctttcgcaa agtgtctcag gcgctggctg
    12181 gtttcttccc cggctggatg ctgacgcaaa ttggctatgt gccgaacgtc gcacaggctg
    12241 accacaccat tgaagggtta cgccagttga tcttcatcta cccaagcgca ctggcggtag
    12301 tcaccattgt ggcgatgggt tgcttctaca gcctgaacga gaagatgtat gtccgcattg
    12361 tggaagagat agaagcccgt aaacgcacgg cgtaataata ataaacgacg ccctgcgggg
    12421 cgttataagg agtgattatg tctgaccata atccactgac attaaaactg aatctgcggg
    12481 aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt aatgctctgc atcggtactc
    12541 tgtatctcct caaactttat accgatgaat tagggatgcc tgcttactat ggcgggatta
    12601 tctttctggt cgcgaagttt tttaccgcgt ttaccgatat gctcaccgga tttttactcg
    12661 actcgcggaa aaatattggg cctaaaggca aatttcgtcc ctttatttta tatgcagcag
    12721 ttccggcggc gttaattgca acgctccagt ttatcgccac caccttttgt ttgccggtta
    12781 aaacgacgat tgccaccacg ctgtttatga tgtttggact ttcatacagt ctgatgaact
    12841 gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg
    12901 cggcttaccg ccagggtggt gcaaccatag ggctattgat ttgtaccgtg gcgtttattc
    12961 cgttgcagtc gcttttttct gactcaaccg tcggttatgc ctgtgcggca cttatgttct
    13021 ccattggcgg ctttattttt atgatgctgt gctacagagg cgtcaaagag cattatgtgg
    13081 acacagcgcc aaccggacat aaagccagta ttctcaaatc tttttgcgcg atatttcgta
    13141 atccaccatt gctggtttta tgcattgcta acctgtgtac tctggcggca tttaatatca
    13201 aactggcgat tcaggtctat tacacccagt atgtgctgaa tgatattaat ttattgtcgt
    13261 ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt attactggtg ccagtaactg
    13321 taaaatgttt tggtaaaaaa caggtttatt tagctggcat ggtgctatgg gcggtgggtg
    13381 atatactgaa ttatttctgg ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg
    13441 ccttttttgg cacggccttt gtaaacagcc tgaactgggc actggtacca gataccgtag
    13501 attacggtga atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc ggttatacct
    13561 tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa
    13621 ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt acagggtttg cgtcagctca
    13681 tttttatctg gccttgtgca ctggcaatta ttgctgcatt gacgatggga ttcttttaca
    13741 cactcaatga aaaacggttt gcattgatta ttgaggaaat caaccaacgc aaaaataaag
    13801 agatggcgac agaaaaaaaa acggcttccg taacgttata aatgtcaata gccgctattt
    13861 ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg gacttattat gaaaaagatt
    13921 aatgcaataa ttttattatc ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc
    13981 gaaaacaggg aggcatataa tcttgcctcc gatcagggcg aggttatgct acgtgtgggt
    14041 tataacttcg atatgggcgc gggtattatg ttaaccaata cctacacctt tcagcgagaa
    14101 gatgaactaa aacatggcta taatgaaatt gaaggttggt atcctttatt caaaccaacc
    14161 gataaattaa ccatccagcc cggtggctta attaatgata agagtatcag ttcgggtggt
    14221 gcagt
//
LOCUS       DAJTRN010000096        14140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRN010000096 DAJTRN010000000
VERSION     DAJTRN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..2884
                     /locus_tag="OO328_003126"
                     /pseudo
     CDS             1..2884
                     /locus_tag="OO328_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2894..6540
                     /locus_tag="OO328_003127"
                     /pseudo
     CDS             2894..6540
                     /locus_tag="OO328_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6601..7008
                     /locus_tag="OO328_003128"
     CDS             6601..7008
                     /locus_tag="OO328_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931599.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7550..8638
                     /locus_tag="OO328_003129"
     CDS             7550..8638
                     /locus_tag="OO328_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5931600.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8649..10928
                     /locus_tag="OO328_003130"
     CDS             8649..10928
                     /locus_tag="OO328_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931601.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10921..12057
                     /locus_tag="OO328_003131"
     CDS             10921..12057
                     /locus_tag="OO328_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5931602.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12054..14057
                     /locus_tag="OO328_003132"
     CDS             12054..14057
                     /locus_tag="OO328_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931603.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3494 a         3716 c         3621 g         3309 t
ORIGIN      
        1 ccagaatggc ggcaaccatt aattgctttg ttattacccg aaaaaccaga aattgcacat
       61 gaaattgccc agcatctact ggggcaaaaa aaattaccct cgcttgagtg gttaaaaata
      121 gtggcgactg atgagcacat tcttgcctca ttagaaaaat atcacgagcc atatgccatt
      181 tttgatgatt actattgtgg tgcgatatgg tcagccaccg tattacagga gcaaggtgtt
      241 gcagccctgc cccgatttgc tccctatgcc gcaagtgact actgcgccga tgtgttgcgt
      301 catatcaatc atccgttcgc attaacactg cttatacgtg tagccgggca tactaaacgc
      361 tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac acgcagcact ggcagcactg
      421 gcggaacttc tagtgcaaaa agaagagaat agttggcgca ttatgctaat gacgatgttt
      481 atctcgcaac cagcactggc agaacaggtc attccctggc tttcgacacc cgcagttgca
      541 gtgctgaaat catgccagca ccaactgaca cagccctcaa accatgccag cgccgatcta
      601 ctgccagcca tagtagtctc cccaccatgg atttcgaaaa agaaaaaatc gccgattccg
      661 gtgctggatt tagcgccatt aggcatcgag ccaatctgtt atctgacaga agaaatcagt
      721 aatcaacttt tggcgaaata tatctggtat tcaaaacaca tcacggttag ccatgaagaa
      781 agtactacca acctgttggc aaggatgggt tttcaacgac ggatcgctgg tacatatatt
      841 aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag attattcaac cttactaagt
      901 gaatttaagg tgtttcattc acctaccggg cattattggc agttggggat tttgacaaca
      961 ttgccgctgg aggctggaga aagcagtaaa agcatggaat gcccttactc tatctccaca
     1021 taccgatacc gaatacgcca tgttacattt tggactcaaa ggtttacctg gtttagttaa
     1081 ctcaattaca cgctatccac aggaagcctt gcccatcacg aattacttcg cagcgagtga
     1141 gctggctcct gccgtcgccc gtgccttcaa caaactcaaa acgctacgcg aaaacgcccg
     1201 cacctggctg ttgaaatacc cggaacatgc ccttaccggc ctgctgcctg cggcgctcgg
     1261 caaagccggt gaagcacagg ataacgcccg cgctgccttg cgtatgctta ccgaaaacgg
     1321 tcatcagcca ttactgcaag aaatcgcccg gcgttataac cagtcggaag taacagacgc
     1381 ggtgaacgct ctgcttgcgc tcgatccctt agataatcac ccgacaaaaa tccccactct
     1441 tccgaccttt tatcagccat cgatctggac gcgcccggta ttaaaagcaa acgcccaatc
     1501 actgccagat aacgccctcc tccacctcgg cgaaatgctc cgcttccctc aggaagaggc
     1561 tctgtatccg ggattattgc aggtgaaaga cgcctgtacc gctgactctc tggctgaatt
     1621 tgcctgggat ctgtttaccg cctggcagac cgctggcgcg ccgtcgaaag agagttgggc
     1681 gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc cgcaaactga cgccattaat
     1741 acgcgcctgg cctggtgaat cccagcataa acgcgccacc gttgggttgg atattctcgc
     1801 cgctatcggt agtgatatcg ccctgatgca gcttaacggc attgctcaga aactgaaatt
     1861 caaagcatta caggagcggg caaaagaaaa gattgccgac attgccgaga gccgcgaact
     1921 cacggtagcg gagcttgaag atcggttagc accggatctc ggtctggatg ataacggttc
     1981 gttgctgctg gattttggcc cacgccagtt caccgtcagc tttgatgaaa ccttaaaacc
     2041 gtttgtgcgt gatgtttccg gcagccgcct gaaagacctg ccaaaaccaa acaaaagcga
     2101 ttatgagtca caggcgaacg atgctgttaa ccgctacaaa ttgctgaaaa aagatgcgcg
     2161 taccgtcgcg gcccagcagg tagcaaggct tgaatccgcc atgtgcctgc gtcgccgctg
     2221 gtcgccggaa aacttccaac tcttcctggt tgtgcatccg ctggttcgcc acttaacccg
     2281 ccgtctgatt tggggcgttt atagcgccga caaccagcta ctggcctgtt tccgggtagc
     2341 ggaagataac agctacagca ccgctgacga tgatcttttc accctgccgg aaggcgatat
     2401 ctctatcggc attcctcacg ttctggaaat atcaccgacg gatgctgtcg cctttggtca
     2461 gctttttgcc gactacgaac tgctaccacc gttccgtcag cttgaccgta acagttacgc
     2521 cctgacagaa gccgagcgca acgccagcga actgacccgc tgggcaggca gaaaatgccc
     2581 gagcggccgg gtcatggggc tggcaaataa aggctggata aagggcgaac cacaggatgg
     2641 aggctggatg atcaaacctc tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt
     2701 tgcggtgggt atatcaccag ccgaacttag cgctgagcag ctcttaagca agctgtggct
     2761 atgggaaggc aaagcggaaa gctatggctg ggggagtaat tcaacacagg aagcgcagtt
     2821 ctccgtactc gatgccatca ccgccagcga gctaattaac gatattgaag ccctgtttga
     2881 ataaggaaag agcatggaca aggaattacc gtggctggcg gataacgccc aactggaact
     2941 gaaatataaa aaaggcaaaa caccactcag tcatcgcaat tggccgggcg aaccagtgcc
     3001 tgttatcact gaaagcatca ttcagacact gggtgatgaa ttgctacaaa aagctgagaa
     3061 gaaaaaaaac attgtctggc gttatgaaaa tttttcaccg gagtggcagt ccgccattac
     3121 gcaggccatc aacttgatcg gcgaacacaa accctcagtc ccggcccgca caatggcggc
     3181 actagccttt atcgcgcaaa atgacaacca acagttgctc gacgaaatcg tccaacaaga
     3241 ggggctggaa tatgcgactg aggtggtgat tgcacgccag tttattgcac ggtgttatga
     3301 gagtgatcct ctggtagtca cattgcagta tcagaacgag gactatggct atggttatcg
     3361 ctcagaaacc tataacgaat tcgatctccg actgcgtaaa cacctctctc tggcagagga
     3421 aagctgctgg cagcgttgcg ccgacaaact cattgccgca ctgccaggaa taaccaaagt
     3481 tcgccgccct tttattgcgc tgatcctccc cgaaaaacca gaaatcgcca atgagttggt
     3541 aagccttgaa tgcccgcgga ctcattttca ttctaaggag tggttaaaag ttgttgctac
     3601 tgaccccaaa gcggtgagaa aactcgaacg ctactggagc caggatatat ttagcgatcg
     3661 agaagccagc tacatgtcgc atgaaaacca cttcggctac gcggcctgcg ctgccctttt
     3721 gcgcgaacaa ggactggcag tcgttccgcg cctcgcgata tatgcccata aagaagattg
     3781 cggcagtctg ctggtacaaa ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc
     3841 tgataaaaac aaacccagcc tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc
     3901 gacactcgcc gcactggcag aactgctggc gttaaaagaa ccaccagccc gcccaggtta
     3961 tccaatcatc gaagacaaaa agctgcctgc acagcaaaaa gctcgagatg aatactggcg
     4021 tacgctgtta caaacgctga tggcatcgca gccacaactg gcagcagaag tgatgccgtg
     4081 gttaagtact caagcccggg cagtgctgaa tagttattta tcggcaccgc ccaaaccagt
     4141 tattgatagc accgataaca gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg
     4201 gagaaagaaa aagacttcca tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca
     4261 aacgtacagg tcgccgaata aacactggac gccggaacag caaactgcaa ttgaccattt
     4321 tagcgccatg tcttttgacg aacgtcttgc tacgagagga gcggatatct tcttgcggga
     4381 attaggtttt gacgttcata gcctggaatt taataagcgt cttcttaatg gacagttgga
     4441 tgcctttgat gctcagcaac tgcaagaata cacatcaata ggactattgc gtacttatca
     4501 cgcgcatcag gccaaatcta aacagtaccg ggaagacgcc accaatgcat tactggcaca
     4561 agatagcatt gcgttgatgg gagggtggcg aacttttcag cacggatttt actatggtaa
     4621 tgaacagcgt tgggggattt ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc
     4681 ggtatcgtgc tggcaacaaa ttgtcgaggc tgattttcat tacaccgggg tggaatattt
     4741 gttaagtgtg ctgggtaccg atgctttgcc tggattaaac accgcctttg cccgacatcc
     4801 aaaagagata tttccgctgc tgattcattt cggcgcaaca gaactggccc tgcccattgc
     4861 ccgcgtctgg cacctttttg cgggccagcg taatctggct cgccagtgga ttttacaatg
     4921 gccggaacat acggctactg cacttatccc tcttgtcttc accaaatcta gcgataaaag
     4981 cgaagctgca ttacttgccc tgcgtttact ttacgaacat gggcatggcg aatggctaca
     5041 aactgtagcc aatcgttggc agcgtacaga tctatggcct gccctggaac aattacttaa
     5101 acagagtcca atagagattt acccggcacg cattccaaaa gcccctgatt tctggcatcc
     5161 gcaaatgtgg tccaggccgc gccttatcac taataatcaa cctgttaccg atgacgctct
     5221 ggaaattatc ggcgaaatgc tgcgctttac ccagggggga cgtttttata gcgggctgga
     5281 acaactgaaa acgttctgcc agccacaaac gctggcagct tttgcctggg atctcttcac
     5341 tgcgtggcaa caagctggtg cccccgcaaa agacaactgg gcatttctgg cgttaagtct
     5401 ctttggtgac gaaagcacgg cacgggatct aacgacacat atcctcgcct ggccacaaga
     5461 aggcaaatct gcccgtgcgg tcagtggcat gaacatcctt accctgatga ataatgatat
     5521 ggcgctgatg cagctgcatc atatatcgca acgggcgaaa tcccgcccct tacgtgataa
     5581 cgcggcggaa tttcttcagg tagtcgcaga aaatcgcggg ctaagccagg aagagttagc
     5641 ggacagatta gtcccaacac tgggccttaa tgatccgcag gcgttgagtt ttgattttgg
     5701 tccccggcag tttaccgttc gcttcgatga aaacctcaac ccggttatct ttgatcagca
     5761 aaacgttcgc cagaaaagtg ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc
     5821 cgaggcactg gcccgactaa aagggttaaa aaaagatgct actcaggtga gcaaaaacct
     5881 gctcccgcgt cttgaagctg ccctacgtac cacccgacgc tggtcgctgg cagattttca
     5941 ttctctgttt gttaatcatc cctttacccg tctggttacc cagcgattaa tatggggggt
     6001 tatccggcaa atgaaccgcg tcgtttactc aacgcctttc gtgtggccgc agagggggag
     6061 ttctgcaatg cgcaagatga gccaattgac ctgcctgcgg acgctctgat tggcattgcc
     6121 cacccgttag aaatgacagc agaaatgcgc agtgaatttg cacagctttt tgccgattac
     6181 gaaattatgc cgccttttcg ccagttgtcg cgccgcacgg tgctgctcac acctgacgag
     6241 tcaaccagta acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg gcaacttatg
     6301 ggaatgcgat acaaaggctg ggagtcaggt tatgaggacg catttgtcta tgacctgggt
     6361 gagtaccggc tggtccttaa gttttcaccc ggttttaacc actacaatgt tgatagcaaa
     6421 gcgctaatga gcttccgttc tcttcgagtg taccgtgaca ataaatccgt cacttttgcc
     6481 gaacttgatg tgtttgattt gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa
     6541 aacaaagcgg gcagtgatgc gactgcccgt tatctacacg acttaccagc ggggaaagcg
     6601 atgatcgtgc aaaaagagct ggttgctatt tacgattatg aggtccctgt acctgaagat
     6661 ccgttttcct tcagacttga gatccataaa tgctctgaat tatttacagg ttccgtctac
     6721 cgactggaac ggttccggct acgtccaaca tttcatcaac gtgatcgaga ggatgttgac
     6781 ccgctaataa atgatgcgtt gatttatata agagatgagt ttattgatga gcaaaaatta
     6841 cgaggcgaat cacctgaacc tgtaatagta atttttaatt gtaaactaca gaatatattc
     6901 aaccaacaaa tagaattata tactctagat aattcaagtc gcagcacatg caacttgaag
     6961 tatgacgagt atagctttat taaagggggg aacggtattc ccccttagat gcgtttcaat
     7021 ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc cactaatagc tccagtacag
     7081 caatgagcac ggttaacttt tgtgtcagcg tgtccatccg ctctccttat taaggagcac
     7141 aacaccagca ccttaccctt acactgcctg tcgatactgg tttacgtttg ttgagtcgcc
     7201 atgttgcact ccggatcaga gcactacctg cggcaccacc cgtccggtag ccaggacttg
     7261 aaataggttc gatccggcgc aacttcccaa cagtcaggat tataaatctc attatcttaa
     7321 ttgcaacact gtccagaggt ggttataatt cgttcgttgg aacacgatgt tgcacaggca
     7381 gtggtgttag gcctgaaaat agtaaataaa agaacctgcc tcaccagcag ttttttttat
     7441 ttactgtgat ctgctttcca gatatttttc gctcaaacaa ctaatgcgcc aaacatttat
     7501 tgcgcgtaaa atatcgttta tttcattaat acatttcagg gatgaatata tgtcaccgca
     7561 gaataatcat ctgcagcgtc cgcctgctgc tgtgttatac gccgatgaac tggcaaaatt
     7621 aaaacaaaat gataacgcac cttgcccgcc cggttggcag ttaagtttgc ctgcggcccg
     7681 tgcttttatc cttggcgata gcgcgcaaaa tattagccgt aaagtggtga ttagtccctc
     7741 cgctgtcgaa cgtatgttag tcactcttgc caccggacgc ggtttgatgt tggtggggga
     7801 acctggcacc gcaaaatctc tcctgtctga attactggca acctctatta gcggcgatgc
     7861 cgggttaacc atccagggcg gcgcatctac taccgaagat caaatcaagt acggctggaa
     7921 ctacgccctg cttatcaatc atggtccatc aacggaagcg ctggtccctg ccccgcttta
     7981 ccagggaatg cgcgacggca aaatcgtccg ttttgaagag ataacgcgta caccgctgga
     8041 agtgcaagac tgtctgttag gtatgctttc tgacagggtg atgacggtgc cggaactcac
     8101 tggtgaagcc agtcagctct atgcgcgaga aggctttaat attattgcca ctgccaatac
     8161 ccgcgatcgc ggcgtcaatg aaatgagtgc tgcgctaaag cgtcgcttcg attttgaaac
     8221 tgtctttccg attatggatt tcgcccagga gctggagctt gtcgccagtg cttcggcgcg
     8281 tttgctggcc catagcggta ttccgcataa agtgcctgac gcggtactgg agttactggt
     8341 ccgcaccttc cgcgatctgc gtgccaacgg cgaaaagaaa acgtcaatgg atacactaac
     8401 agcgattatg tccaccgctg aagccgtgaa cgtggcccac gccgtaggcg tccgcgcctg
     8461 gtttttagcg aatcgcgcag gtgagccagc cgacctggtg gactgcattg ccggaaccat
     8521 cgtcaaagat aacgaagaag atcgcgctcg tctgcgccgt tacttcgaac agcgtgtcgc
     8581 cacacataaa gaagctcact ggcaggctta ttatcaagcc cgccaccgcc tgccgtgagg
     8641 aaagatgcat gagcgagccg ttaattgtcg gcatccggca tcatagtccg gcctgcgccc
     8701 ggctggtgaa atcgttaatc gaaagccagc ggccacgata cgtgttgatt gaaggccctg
     8761 ctgattttaa tgaccgggta gacgaactgt ttttagccca ccagcttccg gtagctattt
     8821 acagttattg ccagtatcag gacggtgcag cccccgggcg tggtgcctgg acgccatttg
     8881 ctgaattttc gccggagtgg caggcgctac aagccgatcg tcgtattcag gcacaaactt
     8941 acttcatcga tttgccttgc tgggcgcaga gtgaagaaga ggacgattcg cctgatacgc
     9001 aagaggaaag ccagaccttg ctgctgcgtg ccacccgcat ggataacagc gacaccctgt
     9061 gggatcactt gttcgaagat gaaagccagc aaactgcatt accctctgca ctggcgcact
     9121 attttgccca actgcggggc gactcccccg gcgatgctct aaatcgtcag cgcgaagcct
     9181 ttatggcccg ctggattgga tgggcgatgc agcaaaataa tggcgacgtg ttagttgtct
     9241 gcggtggctg gcacgctccg gcactggcaa aaatgtggcg cgaatgtccg caggtaatta
     9301 acacgccaga attgccgtcg ctggcagatg ccgttacagg ttgttatctc accccctaca
     9361 gtgaaaagcg ccttgatgtg ctggcaggat acctttcagg aatgcctgcc ccggtctggc
     9421 aaaactggtg ctggcagtgg ggcttacagc aggcgggaga acaactgcta aaaacggttc
     9481 tcacccgttt gcgccagcac aagttgcctg cttcgaccgc ggatatggct gccgctcatc
     9541 tgcatgcgat ggcactggca cagttgcgcg gtcatacact accgttacgc actgactggc
     9601 tggatgccat agcaggatcg ctgattaaag aagccctgaa tgcgccgttg ccgtggagct
     9661 atcgcggcgt tattcatccc gataccgatc cgattctgct aacgttgata gacacattag
     9721 cgggtgacgg attcggtaaa cttgcccctt ctacgccaca accgcctctg ccaaaagatg
     9781 tcacttgcga actggaacgt accgcaatct ctcttccggc ggagcttacc ttaaatcgct
     9841 ttaaccccaa tggactagcg caaagtcagg tgttacatcg gctggcaata ctggagatcc
     9901 cagggattgt acgccagcag ggaagcacac tgacacttgc aggcaacggt gaagaacgct
     9961 ggaaattaac ccgcccgctt agccagcatg cggcattgat tgaggccgcc tgctttggtg
    10021 ccacactcct ggaagccgca cgccataaat tagaagccga tatgctggac gcgggtggaa
    10081 tcggcagcat taccacatgt cttagccaag cggcgtcagc gggtctggcg tccttcagtc
    10141 aacaattact ggagcaactc acattattaa tcgcccagga aaatcaattt gccgaaatgg
    10201 gccaggcgct ggaagtgcta tatgccttat ggcggctgga tgaaattagc ggtatgcaag
    10261 gcgcgcagat attacaaacg acgttatgcg cggctatcga tcgcacgctg tggctgtgtg
    10321 aatccaacgg cagaccggat gaaaaggagt ttcacgctca cttgcatagc tggcaagcgt
    10381 tgtgtcatat cctgcgcgat ctacatagcg gtgtgcaact ccccggcgtt tcgctttctg
    10441 cggcggtagc cttgctggag cgacacagtc aggcgattca tgtcctggca ctggatcgcg
    10501 gcgcgactct tggtgcgctg atgcgtctgg aacatcccaa cgctagtgcc gaagcggcgc
    10561 tgacgatgct ggcgcagtta tccccggcac aatccggcga ggcgctgcac ggtttgctgg
    10621 cattagcccg tcatcaactg gcctgtcagc cggcatttat cgccggtttc agcagtcatt
    10681 taaatcaact aagtgatgcc gattttacca atgccctgcc cgatttacgc gcggcaatgg
    10741 cctggctacc gccacgagaa cgcgggacgc tggcgcatca ggtgcttgag cattatcaac
    10801 tggcgcaact tcccgtttcg gcactgcaaa tgccgttgca ttgtccaccg caagccattg
    10861 cacatcatca acaactcgaa cagcaggcac tggcatcgct gcaacactgg ggagttttcc
    10921 atgtctgaac tgaacgatct tctgaccacc cgtgagctac aacgctggcg attaattctt
    10981 ggcgaagcgg cagaaacgac gctttgtggg ctggatgaca acgcccggca gatagatcac
    11041 gcgctggagt ggctgtatgg gcgcgatcct gaacggctcc agcgtggtga acgctctagt
    11101 ggattaggtg gctcaaatct caccactcct gagtggatca acagtattca cacgctgttt
    11161 ccgcaacagg tgattgagcg gctggaaagc gatgccgtac tgcgctacgg cattgaagat
    11221 gtggtgacaa atctcgacgt gctggaacgt atgcagcctt ccgaaagcct gctacgcgcc
    11281 gttttgcaca ccaaacatct gatgaacccc gaagtactgg ctgccgcccg caggattgtg
    11341 cgccaggttg tggaagaaat tatggcgcga ctggcaaagg aagttcgtca ggctttttct
    11401 ggtgtccgcg atcgtcgtcg ccgctcattt attccactgg cgcgaaactt tgatttcaaa
    11461 agtactctgc gcgccaacct gcaacactgg cacccgcaac acggcaagtt gtatatcgaa
    11521 ttcccccgct ttaacagccg cattaaacgc caaagcgagc aatggcaact ggtcttactg
    11581 gttgatcaaa gcggatcgat ggtcgattcg gtgatccact ctgcggtgat ggcggcctgt
    11641 ttatggcagt tacccggcat tcgtacccat ctggtggcgt ttgacacaag cgtcgttgat
    11701 ctcacggcag acgttgccga tccggtagag ttattaatga aagtgcaatt aggcggcgga
    11761 accaatatcg ccagtgccat ggagtatggt cggcaactta ttgaacaacc tgcaaaaagc
    11821 gtcattatcc tcgtgagcga tttttatgaa gggggttcat catcattgct gacgcaccaa
    11881 gtgaaaaagt gtgtccagag cggcatcaaa gtgctgggac tggcagcgct cgatagcaca
    11941 gcaacacctt gctatgaccg cgatacggcc caggcgctgg ttaatgtcgg cgcacaaata
    12001 gccgccatga caccgggcga gctggcatca tggcttgcgg agaatcttca gtcatgaatt
    12061 cactacgtcc ggaattatta gaactgacac cgcaggcact gacggcgtta agcaatgccg
    12121 gttttgttaa gcgcagcctt aaggaactgg aaaatggcaa cgtcccggaa atcagccatg
    12181 agaacagcgc tttaatcgcc accttcagtg acggtgtccg tacccagctg gcgaacggcc
    12241 aggcactgaa agaggctcag tgcacctatg gggccagcgg catgtgccgt catcgcgtga
    12301 tgctggtgtt aagttatcaa cgactttgtg ctaccgccca gcctacggga aaagaagagg
    12361 aatgggatcc ggcaatctgg ctggaagaac tggctaccct tcccgatgcc acccgcaaac
    12421 gcgcacaggc gctggtcgct aaaagcatca ccattgagtt gttctgtgcg ccgggtgaaa
    12481 ttccctctgc ccgcttaccg atgagcgatg tgcgttttta ttcccgcagc agtattcgtt
    12541 tcgcccgttg tgattgtatt gaaggcacac tttgcgaaca tgtcgtactg gcagtacagg
    12601 cctttgtcca ggcaaaagcg cagcaagcgg aattgactca tttaatctgg cagatgcgca
    12661 gcgaacacgt cacatcatct gacgatccgt ttgccagcga agaaggcaaa acgtgtcgtc
    12721 aatatgttca acaactaagc caggcattat ggctgagcgg catcagccag ccgcttattc
    12781 actacgaggc cgctttcagc cgcgcgcagc aagcagcgga acgctgcagc tggcgatggg
    12841 tgagtgaatc gctacggcaa ctgcgcgcca gcgttgatgc cttccacgcc cgcgccagcc
    12901 actatcatgc cggagaatgc ttacgtcagc ttgcggcatt aaacagtcga ttaaattgcg
    12961 cacaagagat ggcccggagc gacagtgttg gtgaagttcc tcctgtgccg tggcgcacgg
    13021 tcgttggctc tggcattgcc ggagaagcaa agcttgatca tctgcggctg gtgtctttag
    13081 gtatgcgttg ctggcaggat attgagcatt atggtttacg catctggttt accgatcccg
    13141 acaccggcag tattttgcac ctttcgcgca gttggccgcg aagtgaacag gaagactcac
    13201 cggcagctac gcgtcggctg tttagttttc aggctggcgc actggcgggt gggcaaattg
    13261 tttcacaagc agcaaaacgc agtgccgatg gcgagctgct gttagctacc cgcaaccgct
    13321 taagcagcgt tgtaccgctg tcgcctgatg cctggcaaat gttgagcgcg ccgttacgcc
    13381 agccgggcat tgtggctttg cgggaatatt tacgccagcg tccccccgcc tgcatacggc
    13441 ctcttaatca ggtcgataac ttatttattc tgccggtcgc tgagtgtatt tcgctcggtt
    13501 gggatagcag ccgccagacg ctggatgcgc aggtaatcag cggcgaaggt gaagataatc
    13561 tgctgacgtt atcactgcca gcctcagcca gcgcgcctta tgccattgaa cgcatggcgg
    13621 cgcttttgca acaaacagac gaccccgtgt gtctggtttc tggctttgtc agttttgttg
    13681 atgggcaatt gacactggaa ccacaggtaa tgatgacaaa gactcgtgcc tgggcgctgg
    13741 acgcagaaac tgcgcctgtg gtggtatcac taccttctgc cagcgttctg cctgtgccgt
    13801 ctactgctca tcagttgctg atgcgctgcc aggcgttact tattcaactg ctccataacg
    13861 gctggcgcta tcaggaacag agcgctatta atcaggcaga gttgctggcg aatgacctca
    13921 ccgcggtggg tttttatcgg ctggcacatg tgttggcaca atttcgtaat acagaaagcg
    13981 aggcacgggt agaagcaatg aataacggtg ttttgctttg cgaacaatta ttccccatgc
    14041 ttcagcaaca aggatgaaat agtgcttttt actaagagtt ctactccagt tccggactgc
    14101 tcacgccacg gtattaggca tatcctatat agcccctggt
//
LOCUS       DAJTRN010000097        13954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRN010000097 DAJTRN010000000
VERSION     DAJTRN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            181..2028
                     /gene="ldtD"
                     /locus_tag="OO328_003133"
     CDS             181..2028
                     /gene="ldtD"
                     /locus_tag="OO328_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5931604.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2209..2757
                     /locus_tag="OO328_003134"
     CDS             2209..2757
                     /locus_tag="OO328_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5931605.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2784..3431
                     /gene="gloC"
                     /locus_tag="OO328_003135"
     CDS             2784..3431
                     /gene="gloC"
                     /locus_tag="OO328_003135"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5931606.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3654..4844)
                     /gene="aspC"
                     /locus_tag="OO328_003136"
     CDS             complement(3654..4844)
                     /gene="aspC"
                     /locus_tag="OO328_003136"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5931607.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5029..6117)
                     /gene="ompF"
                     /locus_tag="OO328_003137"
     CDS             complement(5029..6117)
                     /gene="ompF"
                     /locus_tag="OO328_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5931608.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRAGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6718..8118)
                     /gene="asnS"
                     /locus_tag="OO328_003138"
     CDS             complement(6718..8118)
                     /gene="asnS"
                     /locus_tag="OO328_003138"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5931609.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8287..9489)
                     /gene="pncB"
                     /locus_tag="OO328_003139"
     CDS             complement(8287..9489)
                     /gene="pncB"
                     /locus_tag="OO328_003139"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5931610.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9755..12370
                     /gene="pepN"
                     /locus_tag="OO328_003140"
     CDS             9755..12370
                     /gene="pepN"
                     /locus_tag="OO328_003140"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5931611.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12577..13344)
                     /gene="ssuB"
                     /locus_tag="OO328_003141"
     CDS             complement(12577..13344)
                     /gene="ssuB"
                     /locus_tag="OO328_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5931612.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13341..>13954)
                     /gene="ssuC"
                     /locus_tag="OO328_003142"
     CDS             complement(13341..>13954)
                     /gene="ssuC"
                     /locus_tag="OO328_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5931613.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3547 a         3560 c         3524 g         3323 t
ORIGIN      
        1 cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt tatatactga
       61 agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt ggcgttgtac
      121 aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag ggggcaaggg
      181 atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg cctggccgta
      241 acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc tggcgacagc
      301 ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac ggcaataatg
      361 gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca aatcgaatct
      421 caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt gtatgccgca
      481 cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg
      541 gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga gttactgacc
      601 gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat gatgggctat
      661 ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag cagtaaacct
      721 tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc gctggataaa
      781 ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata tgcggcgatg
      841 catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact gaccggcaaa
      901 gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa
      961 cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact
     1021 gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac
     1081 aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta cgataatgaa
     1141 ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga tggtgctatt
     1201 ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggca
     1261 ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac
     1321 attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga ttcgcgagtc
     1381 attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa caacgtagtg
     1441 gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct gccgaaagtg
     1501 cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg ctggaacagc
     1561 agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc gaatttaccg
     1621 tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg
     1681 ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat
     1741 acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg
     1801 ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa gcagggtgat
     1861 acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt
     1921 gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg
     1981 cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc
     2041 ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc agcccccgtc
     2101 actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc
     2161 gccagaagtg catataaacg ataacattga gctgtagact tgattatcat ggacaaattc
     2221 gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg
     2281 ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat
     2341 cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa
     2401 gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc
     2461 gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg
     2521 gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg cgcccgcagc
     2581 cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa
     2641 ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga
     2701 tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagtaa
     2761 tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc gcattctccc
     2821 agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc gatcctggcg
     2881 gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc
     2941 tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg
     3001 gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc
     3061 aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg
     3121 aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc cctgggcata
     3181 cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta
     3241 ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt
     3301 cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt ccgggtcacg
     3361 gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa gacgaaatgc
     3421 ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat ttctggcaaa
     3481 gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca aaatcgtgca
     3541 aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca ggcaatgttg
     3601 cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa cgcttacagc
     3661 actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc ggccacattt
     3721 acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag cacttgttct
     3781 tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct gaagtcgcgg
     3841 tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg aatacgctgg
     3901 cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct caggatggtg
     3961 gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat cgccgctttc
     4021 atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt acaagcgcca
     4081 acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat cagctcttta
     4141 tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg ggcaaaaccc
     4201 tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg tgccagtgtc
     4261 tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc atggaacagc
     4321 actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa gtcaagggtg
     4381 tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga gttaaagacg
     4441 ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct ggtatttttt
     4501 gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg tgccgtgcga
     4561 gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg agtgcagcga
     4621 ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt ttcgagcaga
     4681 tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc atctttatag
     4741 acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag atcggccagg
     4801 cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag gttccattat
     4861 ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt ccaactatta
     4921 ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg cataaaaaaa
     4981 acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt agaactggta
     5041 aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt cagaatcgat
     5101 ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt aggttgcgcc
     5161 cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt tcgctttaga
     5221 tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg caactaacag
     5281 aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag tgatcggcgt
     5341 agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt cgtacttcag
     5401 accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt cttgcaggtt
     5461 ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt cgtagctgat
     5521 agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt ttttacccag
     5581 gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt tacgataggt
     5641 agcaacgccg ccagcacgac caacgaagaa gtcatcgctg tatgcagtat caccaccaaa
     5701 ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt tacggccgta
     5761 atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg ttttgttacc
     5821 agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc actgaccata
     5881 accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg cataggtcat
     5941 gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat gcagaccaac
     6001 agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg cagcgtttgc
     6061 agtacctgct actaacagag cagggacgat cactgccaga atattgcgct tcatcattat
     6121 ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt tctgtcaata
     6181 aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc
     6241 attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt agtttcaaaa
     6301 agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg tcatatttcc
     6361 ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta cttatattta
     6421 aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc acaaaatcct
     6481 ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa
     6541 actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa tgtaatattc
     6601 cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta acaaacagat
     6661 aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca
     6721 gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc
     6781 agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc
     6841 gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat
     6901 acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga tctcaccgat
     6961 gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata
     7021 gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc
     7081 cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa
     7141 cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc
     7201 ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa
     7261 tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc
     7321 agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa
     7381 ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata
     7441 aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa
     7501 agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag
     7561 gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc
     7621 ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc
     7681 ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag
     7741 gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc
     7801 tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa attgttgccc
     7861 ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg
     7921 caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa
     7981 gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg
     8041 tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc
     8101 tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc
     8161 cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac aagcagaatt
     8221 cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat tagctccctg
     8281 atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac
     8341 aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt
     8401 accgttacac tctaccaact taatcacaat attcaggggt tttacctggg ggatatcgca
     8461 ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag
     8521 ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac tctgtggatc
     8581 aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga
     8641 gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac gcaggaaagc
     8701 atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact cttccagcca
     8761 ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct gatgtgcctg
     8821 gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta
     8881 gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac
     8941 ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg acatatcaag
     9001 accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc
     9061 aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg
     9121 aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca gcttgccatt
     9181 atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc agttaagata
     9241 gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct gcaggcgcag
     9301 gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc
     9361 acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg
     9421 ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa
     9481 ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa taaacatctt
     9541 caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc
     9601 cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt
     9661 ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa
     9721 caccagactc tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata
     9781 ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct
     9841 cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga
     9901 tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga
     9961 gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta atttgccgga
    10021 gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg cgctggaagg
    10081 gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt tccgccatat
    10141 tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa ttattgccga
    10201 taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag gcgaactcga
    10261 aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct acctgtttgc
    10321 gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt ctggtcgcga
    10381 agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt gggcgatgac
    10441 ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt atgacctcga
    10501 catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga ataagggtct
    10561 gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg acaaagatta
    10621 cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg gtaaccgagt
    10681 gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct tccgcgatca
    10741 ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg tgcgcaccat
    10801 gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc gcccggacat
    10861 ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg cggaagtgat
    10921 ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc aactctattt
    10981 cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga tggaagatgc
    11041 gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta cgccggttgt
    11101 gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca tcagccagcg
    11161 cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt ttgccatcga
    11221 attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc cggtgaattc
    11281 cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct acttccagcc
    11341 ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata agtggagcga
    11401 tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt gggatgcggc
    11461 gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc aagggcagcc
    11521 gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg atgagaagat
    11581 tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa tggctgaact
    11641 gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca ctcgtactct
    11701 ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc agagcgagta
    11761 ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc tgcgcttcct
    11821 tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc atgaagcaaa
    11881 caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc tgccttgccg
    11941 tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg tgatggataa
    12001 atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg tgcgtggtct
    12061 gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt tgattggtgc
    12121 gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt accagttcct
    12181 ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac gtctgattga
    12241 accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc gcgcggcgct
    12301 ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga taactaaagc
    12361 actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt cttatcaggc
    12421 ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac gccgcatccg
    12481 gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc tacaagtccg
    12541 ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc gaatcagttg
    12601 ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct ccgctaatcg
    12661 caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac taatttttcc
    12721 ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat catgcgtcac
    12781 caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct gcatctcgag
    12841 tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac ccggtcgatg
    12901 aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg cggcaggcca
    12961 ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg catcgcgcca
    13021 ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca gcagacgcgc
    13081 atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg tgcccgctaa
    13141 cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg tacttttacc
    13201 accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta aatccagttg
    13261 gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca atggtgtgcc
    13321 ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc cgggttccag
    13381 cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc gagcagggcg
    13441 taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc attcatcgcc
    13501 agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt cagccacatc
    13561 aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg caggatcaca
    13621 tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac cagcccgcga
    13681 tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt accgagcgcc
    13741 accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag cgggatcagc
    13801 gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct ttctccccag
    13861 cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat tgaaaagcca
    13921 atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJTRN010000098        13735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRN010000098 DAJTRN010000000
VERSION     DAJTRN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            271..1062
                     /locus_tag="OO328_003143"
     CDS             271..1062
                     /locus_tag="OO328_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931614.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1448..1654
                     /locus_tag="OO328_003144"
     CDS             1448..1654
                     /locus_tag="OO328_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5931615.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1740..2312
                     /locus_tag="OO328_003145"
     CDS             1740..2312
                     /locus_tag="OO328_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931616.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2314..2598)
                     /locus_tag="OO328_003146"
     CDS             complement(2314..2598)
                     /locus_tag="OO328_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931617.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2569..2712
                     /locus_tag="OO328_003147"
                     /pseudo
     CDS             2569..2712
                     /locus_tag="OO328_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3881..4900
                     /locus_tag="OO328_003148"
     CDS             3881..4900
                     /locus_tag="OO328_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5931618.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5010..5882
                     /locus_tag="OO328_003149"
     CDS             5010..5882
                     /locus_tag="OO328_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5931619.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6216..9338
                     /gene="ag43"
                     /locus_tag="OO328_003150"
     CDS             6216..9338
                     /gene="ag43"
                     /locus_tag="OO328_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5931620.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9459..11975
                     /locus_tag="OO328_003151"
     CDS             9459..11975
                     /locus_tag="OO328_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931621.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12051..12506
                     /locus_tag="OO328_003152"
     CDS             12051..12506
                     /locus_tag="OO328_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931622.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(12625..12836)
                     /locus_tag="OO328_003153"
                     /pseudo
     CDS             complement(12625..12836)
                     /locus_tag="OO328_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12917..13735
                     /locus_tag="OO328_003154"
     CDS             12917..13735
                     /locus_tag="OO328_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5931623.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         3671 a         3427 c         3410 g         3227 t
ORIGIN      
        1 atgaactacc tgtagctgta acgataaaca aatcacccaa aatgaaaaat actggttatc
       61 acagttcaga gaactcgtat gagtgtcccc ctgagcaatg cttcattttc tgtgattctg
      121 tgtgatagag ttatactgag tacagtggca gtaacactgc tttccatcac ttgaggataa
      181 aattcattaa aaaattaatt gcatgaatta acaggttttt ttatgaaatg ttatgcatct
      241 caaccaatat gattaacaga aaacttcatc atgacaacac gtataacgtt cagtatgacc
      301 agtgatgaga cgctgcgaat tgtggatgag tactgtcata cccacaaact ttcgcgctct
      361 aaggtaatca acgcattact cagcgccaca gcacctgtcc ttaatgatat taactgttat
      421 tatcaacttg caggcgaact gcagtcacgc ttattaaatg gagtgtatca acgggatctt
      481 cctcgcaaac gcaatgtcgt cagtgcagag aagtattgtc tggaaatctg ggaaaacaaa
      541 cttttcacca gaagaatcct ggagtttgac agttcaaatg gtgttctgta cgcgctgaaa
      601 cataaaagac actatcgccg ggataagatg attggaaggg ttgaaagtcg gtgtatcaaa
      661 gacatttgcg aataccagat gcagctatcc ggggagaaga cgaaatatgc ctgttttatt
      721 tatatcgaaa gaacaatata caatcacgat aatccatctg ataaaatacc tgtaaaagcg
      781 gctgttggta atgcagtcat cctgctggca aaggatgtga tatataacga gtactttttt
      841 gatttgcggc agtcattttt tgtgagcgtt acagatctga tggtctctgg cgcaaaaggt
      901 attccggaga cacagacata tccggacgtt tattgctgga tccctctttt ttccataaat
      961 tccggcgtgc tcattacccc tgtatataaa attgaccctc tgaaacccgt aacggtaaaa
     1021 aaaccggata agatcactgt tgtttgtaat tacagagaat aatttcagcc ttctaccttc
     1081 accgcgacag tgtttacctc tgctttatct tccgttcagt aaatacaaac caccgctctg
     1141 gcaggtacag ggcgtggttt atctccgata ctcccctgtg cagtaataat catcactgaa
     1201 aatactgatc ccgtcacggc aaaacgccat cccatattcc ggcaacacaa aatcatcttc
     1261 cgtttatacc ggattcactg acaaaacaga caacctgtat tgcctgtaca caacagtttc
     1321 tctcctggca ggaatattcc cctactcatt ttttacgttt ctccccggtt gttatgccgg
     1381 tgaaggtggt gcgtcgtttt catcaccaac gccggttgag attaacaaca tccggaggaa
     1441 cattctcatg aataccccag tttcactgat ggatgaccag atggtcgaca tgtcgtttat
     1501 cactcagctg accggcctga ccgataagtg gttttataaa ctcatcaagc atggagcctt
     1561 tccgcccccc atcaaactgg gccgtagctc ccgctggctg aaaagcgaag tggaagcctg
     1621 gctgcaggcg cgtattgcac agtcccgtcc gtaattcctg accgttatcc gttccaccgc
     1681 agcaaaggca tgatccggcc cccggaggca ttttgctgcg cgctaaagga gagaatatta
     1741 tgcaacaact tgaaaacagg tgtgacctgc tgctcatcca gcatcagaaa tggatgacaa
     1801 gcgtgacgag gcttatcgtc gctcatggaa tggggtctcc gcatcttcat ggttatcacc
     1861 gtctgaccct ggcacacttt tttctgccgg aaaaaggctc cgtcatttcg gtggcccccc
     1921 agggactcta tcaggtcgtc aacccgggaa caccaccgtt cattcctgcc attcaggaag
     1981 ggttaatgac cagcattcag acccatgaaa tcatgctgct tacacatttt aatctgggcg
     2041 gcgttttact cagtgagcta catcgtctgg gtgaaaaccg gctggcaaac aggctgaaca
     2101 gcctgctccg gcgtttcgac gatcgtgatc tctaccacac gctgatatgg ctgtgctggt
     2161 atgatctgat gtgtgcccac tccatgcagc cctggactga agagttaaag cataaatcac
     2221 atgcagagct tgaaaactgg gctgtggcgc gcaaacggga aaagcgcgag ctggaactga
     2281 tgattgacga gtacctgtta tacgcctgct gattcataac gttcccgggg ttccttcctc
     2341 atcgttacac acagccggac ggctatctat ggctgtccgc caaatgacca tcgccgcatt
     2401 tcgttccgct gaaaacggta cgtagtcagt gataccggaa tttccggaga gggccgcagg
     2461 tatattgctt ctgtgcaatt gatgcttatt tacattggat gtagttattt ctgcatccaa
     2521 tgctatcgga aagagattaa ccagcaaatg gttcctcaaa tcgtattgat atttacccgg
     2581 cgtattgagc accaccatct gacactccgt tcccgcatca aacgactgga ccgaaaaacg
     2641 atttactttt cacgtgctgt ggaaattcac gaaaaaatta ttgggtcatt catcgaaaaa
     2701 catatgttct gatgactgga gtcatcctcc agaaatatac gtcaactgaa caatatcgat
     2761 tttttatggg attctataca cttttccata tccctctgaa aatcagtcat cagctcacca
     2821 acacctcttc tgaaacttgt attaaactta ttgcttaaat agatgatata acatctcttc
     2881 cattgcactg aatcggcgaa aactgccgtt tcaataatct caacaatatt atttatcggc
     2941 taactttatc agtaaacaat ggaatgtcac actgatgaaa tgaacattca gagtaagatg
     3001 ataaaattca tataaacgcc attgctgaat attatttatt tcatacagat aaacgataaa
     3061 atacaaaagg gtatatttct caatgtaaca gtcaattatt catctccact ggcgagcacg
     3121 gtcagcacac aaacaaagtt agtcctcctg cttaatcatt gcgtatcaga aactaaaaaa
     3181 gaaaaaattc tgcagacaac ttaacaatct tcatcacatt tcctgaagac agacatatcc
     3241 acaaacggta gtcagcgttg aattaacaag tcttacagag ctatacgtag cttataaagc
     3301 cactcaatgt tcatcatcac ccgactactt atctggaata ccaccagtat aaactcatca
     3361 ataaaaacac ctgttgttaa aataagaata ctgtcagcag taaattatta aaacaacatc
     3421 tgtccacact atctgttctt ttaacgccaa cggcagcgaa aatagtgcat cacagccttc
     3481 ttttttcttt cgccaccact cagacaaaca caacactcac acccacacca caaaacagaa
     3541 tcaaaacata aaattaacaa taacagtcaa aaggaaaaca cagaatattg aggtggactg
     3601 tcaccaggac agcgcatacg acgcatgtga tcagcacatc ccgttcttcg ctggcatccc
     3661 caaaatgcag aacaaacaat cataacatta tgaatataaa agaaaatatg atttttcacc
     3721 atgaaaaaaa catcaatgta tctctgtata aaaataatga taacatgaca taaacattta
     3781 aaaacataaa taaacagcaa caaaaacatt tgataatcac ttagtgatta aatacaatta
     3841 agacgatttt gttataagag aggcaccatg gagtgagata atgaataacg aacagatgct
     3901 tatcaaatgc agagatcccc gtaaatgtcg gttttatgcc gaatatctga atcacaaata
     3961 ccctaatgaa ttccctcact ttgcgcctga caacccacaa aattgtatca ggaatgttaa
     4021 tttattttta aaggaaaagt attgtaatgt ttcacctccc ccctttggta cttttggtac
     4081 tactgaagaa gaagcactca ataacgaaat taatgagctt gaaattattg ccgggcaggc
     4141 aatactgcct gaagagagtt ttctctggtt gaaaactgac gaacttgcga ctttctttac
     4201 ctggactact atttatctgt cgaaggaaga ccatacaaaa ttcccgaaaa taaaccataa
     4261 ttcatcagga ataaaaaacg gaattgaagt caggaaaata cgaacagtta ccgatgaaac
     4321 gttaaacact tacaaaaaaa gtaaccttcc ggataatcca tcatcccata acgaacgaat
     4381 aaaagtaatc atttcatatt ttgactcctg ccattcttta attaataaaa atgcaaaaag
     4441 catatacctc gaggaactca ggcaggcgtg gcttaaaaag aaaagcgctt gtcaaaagtt
     4501 taagtggctc cttcctgacg atgatgaaat gtgtcagtgg ttctggacaa gactgaaaca
     4561 aaaacaggat gaaaaacatc cagtacataa cccagtgacc cggtggttca taccatcttc
     4621 aacgaaagaa tattatcttg cctctcttgt cgcctttgct ttatggaaag aagcgcctga
     4681 taccattgaa ctgttcagaa cacgaataaa tcatgcctgg catcagaaaa aacaaaagga
     4741 caaatatcgg agtgatggta aaaaggccat taatatacat ataagaagca atgtgaaaga
     4801 aatgcttgat gagctcagaa ctgtttacgg tctgagaaca ggagagtttt ttgaaatgct
     4861 gattacagag aaatatcttg aacacaagcg aaggaagtaa ttcattacct gttacattct
     4921 aaataatctc agacagatat tatctccgtg aatcccccgc cacctttccg gtgcgcgggg
     4981 ttttgtcttt tttcaccggg aatacatgta tgaatccgtc tgatgccatt gaggcaattg
     5041 aaaaaccgct ctcctccctg ccttactcac tttcccgtca catcctggaa catctgcgca
     5101 aactcaccag tcacgaaccc gtgattggca ttatgggtaa aagcggggcc ggtaaatctt
     5161 cactctgtaa tgcactgttt cagggggagg tcacaccggt cagtgatgtt cacgccggca
     5221 cccgggaagt gcagcgcttc cgtctgagtg gtcacggtca cagcatggtt atcactgacc
     5281 tgcccggggt gggcgagagc cgggacaggg atgcagagta tgaagccctg taccgtgaca
     5341 ttctgcctga actggacctg gtgctgtggc tgattaaagc cgatgaccgg gccctgtctg
     5401 tggatgagta tttctggcga cacatcctgc agtgcggaca tcagcaggtg ctgtttgtgg
     5461 tgacgcaggc tgacaaaacg gaaccctgcc atgaatggga tatggcaggt attcagcctt
     5521 ctcccgcaca ggaacagaac attcgtgaga aaacggaggc ggtattccgt ctgttccggc
     5581 ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg ggaactggat acgctggtca
     5641 gtgcgctcat gacagcgctt cccgaccatg ccgccagccc cctgatgacc cgactgcagg
     5701 acgagctgcg cacggagtct gtccgctctc aggcccgtga acagtttacc ggtgcggtgg
     5761 accggatatt tgacacagcg gagagcgtct gtgttgcctc tgttgtccgt acggccctgc
     5821 gcgctgttcg tgacaccgtg gtctctgttg cccgcgcggt atggaactgg atcttcttct
     5881 gaacctgttg tggatgatgt cctccctgcc tctgagtctg ctcacaaaag cgctgttttc
     5941 gttactgtct ctcttgtccg tgcaatagac tgataataga ataaaacgat cgatacctat
     6001 tttaccgatc gttaatatcg atcgttatgc taataataac tcctgttagc aacgtgcgca
     6061 gatacacaca gacatgagat tcagggaaca acagagccac acgtcagaaa cttccgtcag
     6121 ctggacctcc ggcactgtaa ccctttacct gccggtatcc acatctgtgg ataccggctt
     6181 ttttattcac cctcactctg attaaggaaa tgctgatgaa acgacatctg aatacctgct
     6241 acaggctggt atggaatcac attacgggcg ctttcgtggt tgcctccgaa ctggcccgcg
     6301 cacagggtaa acgtggcggt gtggcggttg cactgtctct tgccgcggtc acgtcactcc
     6361 cggtgctggc tgctgacatc gttgtgcacc cgggtgaaac agtgaatggc ggaacactgg
     6421 taaaccatga caaccagttt gtatccggaa cagctgatgg cgtgactgtc agtaccgggc
     6481 ttgagctggg gccggacagt gacgaaaaca ccggcgggca atggataaaa gcgggtggca
     6541 caggcagaaa caccactgtc accgcaaatg gtcgtcagat tgtgcaggca ggaggaactg
     6601 ccagtgatac ggttattcgt gatggcggag ggcagagcct taacggactg gcggtgaaca
     6661 ccacgctgga taacagaggt gagcagtggg tacacggggg agggaaagct gccggtacaa
     6721 ttattaacca ggatggttac cagaccataa aacatggcgg actggcaacc ggaaccatcg
     6781 tcaacaccgg tgcagaaggt ggtccggagt ctgaaaatgt gtccagcggt cagatggtcg
     6841 gagggacggc tgaatccacc accatcaata aaaatggccg gcaggttatc tggtcttcgg
     6901 ggatggcacg ggacaccctc atttacgccg gtggtgacca gacggtacac ggagaggcac
     6961 ataacacccg actggagggg ggtaaccagt atgtacacaa cggtggcacg gcaacagaga
     7021 cgctgataaa ccgtgatggc tggcaggtga ttaaggaagg aggaactgcc gcgcatacca
     7081 ccatcaacca gaaaggaaag ctgcaggtga atgccggcgg taaagcgtct gatgtcaccc
     7141 agaacacggg cggtgcactg gttaccagta cagctgcaac cgtcaccggc acaaaccgcc
     7201 tgggagcatt ctctgttgtg gagggtaaag ctgataatgt cgtactggaa aatggcggac
     7261 gcctggatgt gctgaccgga cacacagcca ctaatacccg cgtggatgat ggcggaacgc
     7321 tggatgtccg caacggtggc accgccacca ccgtatccat gggaaatggc ggtgtactgc
     7381 tggccgattc cggtgccgct gtcagtggta cccggagcga cggaaaggca ttcagtatcg
     7441 gaggcggtca ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg ctgaacgccg
     7501 gtgatacggc cacggatacc acggtaaatg gcggactgtt caccgccagg ggcggcacac
     7561 tggcgggcac caccacgctg aataacggcg ccatacttac cctttccggg aagacggtga
     7621 acaacgatac cctgaccatc cgtgaaggcg atgcactcct gcagggaggc gctctcaccg
     7681 gtaacggcag cgtggaaaaa tcaggaagtg gcacactcac tgtcagcaac accacactca
     7741 cccagaaagc cgtcaacctg aatgaaggca cgctgacgct gaacgacagt accgtcacca
     7801 cggatgtcat tgctcagcgc ggtacagccc tgaagctgac cggcagcact gtgctgaacg
     7861 gtgccattga ccccacgaat gtcactctcg cctctggtgc cacctggaat attcccgata
     7921 acgccacggt gcagtcggtg gtggatgacc tcagccatgc cggacagatt catttcacct
     7981 ccacccgcac agggaagttc gtaccggcaa ccctgaaagt gaaaaacctg aacggacaga
     8041 atggcaccat cagcctgcgt gtacgcccgg atatggcaca gaacaatgct gacagactgg
     8101 tcattgacgg tggcagggca accggaaaaa ccatcctgaa cctggtgaac gccggcaaca
     8161 gtgcgtcggg gctggcgacc agcggtaagg gtattcaggt ggtggaagcc attaacggtg
     8221 ccaccacgga ggaaggggcc tttatccagg ggaataagct gcaggccggt gcctttaact
     8281 actccctcaa ccgggacagt gatgagagct ggtatctgcg cagtgaaaat gcttatcgtg
     8341 cagaagtccc cctgtatgcc tccatgctga cacaggcaat ggactatgac cggattctgg
     8401 caggctcccg cagccatcag accggtgtaa gcggtgaaaa taacagcgtc cgtctcagca
     8461 ttcagggcgg tcatctcggt cacgataaca acggcggtat tgcccgtgga gccacgccgg
     8521 aaagcagcgg cagctatggc ttcgtccgtc tggagggtga cctgctcaga acagaggttg
     8581 ccggtatgtc tgtgaccgcg ggggtatatg gtgctgctgg ccattcttcc gttgatgtta
     8641 aggatgatga cggctcccgt gccggcacgg tccgggatga tgccggcagc ctgggcggat
     8701 acctgaatct gatacacaac gcctccggcc tgtgggctga cattgtggcc cagggaaccc
     8761 gccacagcat gaaagcctca tcggacaata acgacttccg cgtccggggc tggggctggc
     8821 tgggttcgct ggaaaccggt ctgcccttca gtatcactga caatctgatg ctggagccgc
     8881 aactgcagta cacctggcag ggactctccc tggatgacgg ccaggataac gccagttatg
     8941 tgaagttcgg gcatggcagt gcacaacatg tgcgtgccgg cttccgtctg ggcagccacc
     9001 acgatatgaa ctttggtaaa ggcacctcat cccgtgacac cctgcgcggc agtgccaaac
     9061 acagtgtgcg tgaactgccg gtgaactggt gggtacagcc ttctgttatc cgcaccttca
     9121 gctcccgggg ggacatgagc atgggtacag ccgcagccgg cagtaacatg acgttctcac
     9181 cgtcacagaa tggtacgtca ctggacctgc aggccggact ggaagcccgt gtccgggaaa
     9241 atatcaccct gggcgttcag gccagttatg cccacagcat caacggcagc agcgctgaag
     9301 gttataacag tcaggccaca ctgaatgtaa ccttctgata attctgcatt gcctctctgt
     9361 ggtcccggtc atgatgaccg ggacccggac gggggaaaac gtttcagtgc cacattcact
     9421 ggcattcaca ataacatgat attcatcacg gagtgactat gttacagata gtcggcgcgc
     9481 tgattctgct gatcgcagga tttgccattc ttcgcctttt gttcagagca ttaaccagca
     9541 cagcgtctgc gctggcaggg ttcatattgc tgtgtctgtt cggtccggct ttactggctg
     9601 gctatatcac tgaacgcata acccggttat tccatattcg ctggctggca ggcgtatttc
     9661 tgacgattgc cggaatgatc atcagcttca tgtggggact tgatggtaaa catatcgcac
     9721 tggaggctca tacttttgac tctgtaaaat ttattctgac caccgctctc gccgctggtc
     9781 tgctggctct tcccgtgcag ataagaacca ttcagcagaa cgggctcaca cctgaagata
     9841 tcagcaagga aattaacggg tattactgct gtttttatac tgcttttttc cttatggcgt
     9901 gttctgcata cgcaccattg atcgcattgc agttcgatat ttcaccctca ctgatgtggt
     9961 ggggcgggtt gttgtactgg ctggctgcat tagtgacgct gctatgggcg gccagccaga
    10021 tccaggcgct gaaaagactg accagtgcca tcagccagac actggaagaa caaccggtgc
    10081 tcaacagtaa atcgtggctg agcagtttgc aaaacgatta cagccttcct gaaacgctga
    10141 cggagcgcat ctggctgaca ctcatttcac aacggatttc ccggggagaa ctgagggaat
    10201 ttgaactggc agacggaaac tggttactga acaatgcctg gtatgaaaga aacatggcag
    10261 ggtttaacga acagttgaaa gagaacctgt cattcacacc tgatgaactg aaaacgctct
    10321 tccggaaccg cctgaattta tcaccggaag cgaatgacga ttttctcgat cgttgcctgg
    10381 acggcggtga ctggtacccc ttttcagaag gccgccgttt tgtatcattc caccacgtgg
    10441 atgagcttcg tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc ccggaaaatc
    10501 ataagccggc tccggaatgg tactgctcct ctctttgtca cgaaacagaa acactgtgtc
    10561 aggacattta tgaacgttct tacaccggtt ttatttccga tgcaacggcg aatggtctga
    10621 ttctcatgaa actgccggaa acctggagta caaatgagaa aatgtttgct tccggagggc
    10681 agggacatgg gtttgccgct gaacggggaa accatattgt cgacagagtc cgtctgaaaa
    10741 acgcacggat cctcggtgat aataatgcca gaaatggagc agacagactg gtcagcggaa
    10801 cagaaatcca gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg gcattcgacg
    10861 gacaaaacgg acagtatcgt tacatgggaa atcatggtcc catgcaactg gaagtccccc
    10921 gtgatcagta tgccggcgct gtggaaacca tgaagaataa gatccgcgaa ggtaaagtac
    10981 ccggtgtaac cgatcccgca gaagcgtccc ggctgattcg tcggggacat ctgacttata
    11041 cccaggcccg taatatcacc cggttcggga ccatcgaatc ggtcacttat gatattgccg
    11101 aggggtcggt tgtcagtctg gcggccggag ggatcagttt tgccctgacg gcatcggtct
    11161 tctggctcag caccggcgat cgcgatgctg ccctgcagac agctgctgtc caggcaggaa
    11221 aaaccttcac ccgcacactg gctgtctacg tcacaaccca gcaacttcac cggctcactg
    11281 ttgttcaggg tatgctgaag catattgatt tttcgacggc cagcccgact gtccggcagg
    11341 cgcttcagaa ggggaccggt gcaggaaata tcagtgccct gaacaaagtg atgaagggta
    11401 cgctggtgac atctctggca ctggtagctg tcacaaccag ccctgacatg atcaaaatgt
    11461 tgcggggacg gatctccggt gcgcagttca tcaggaatct tgccgtggca tcttccggtg
    11521 tggcaggtgg tgctgtcggg tcagtggcgg gcgggatatt gttcagtcca ctgggaccat
    11581 ttggtgcact gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg
    11641 tatcaggaaa aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga
    11701 ttcaggagca ggtgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa
    11761 atctgaacgc gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg
    11821 ccggtataca acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca
    11881 tcaggcaacg ccccgtcatg gaatatgatg catcccatct cggcaagatg gttaaccggc
    11941 tggaagaagc attccccccg gagctcccgg cataacgtgt gttctgctcc ttttaggttt
    12001 tatcacctga tttgccagtg tcatcgtcat gactcagaga ggtaaatacc atgattcacc
    12061 tgttcaaaac ctgcatgatt accgccttca ttctggggtt aacgtggtct gccccactcc
    12121 gggcacagga tcaacgttac atcagtatac gcaatacaga tacgatatgg ctcccgggaa
    12181 atatttgtgc ttaccagttc cggctggata atggcggaaa cggtgaagga tttggccccc
    12241 tcaccatcac tctgcaactc aaagacaaat atggtcagac gctggtgacc agaaaaatgg
    12301 aaacggaagc ttttggtgac agtaatgcca cgcgaaccac agacgcattt ctggaaacgg
    12361 agtgcgtgga aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa agtaacggcc
    12421 atcgtgtcag tctgccgtta tcggttttcg atccccagga ctaccatcca ctgctgatta
    12481 ccgtttccgg aaaaaatgtt aactgatccc tgaagtcagt ttcatgcttc agcctctgcc
    12541 acaacgtatg gcagaggcta tttatcagga gaaaccagat atgccgggtt acacacagag
    12601 tatgtcctgg ctgaaggatc tttctcatac gggcaggcgg ttgctgtcat aaccgcttac
    12661 cgcaatgtgt ttatccagga tgacccggga atgcatttcc gtcgggttat ccgcaacgcg
    12721 gaaggtcagc gacgctgacg cagccggaac tctgaagctg atgccggaaa acagcttaat
    12781 gcgtggctcg ccagtggagg gcttctcaga caataaactt cattgatttc atccatcagg
    12841 ccgcgtcttc tccgggagac gcggcttttt tcatttgttg caccagtaaa tcttaaccac
    12901 cgataaggag caaagtatgc aattagtcag tcgttttggt tatgcgaacc agatacgccg
    12961 tgaccgtccg ctgacacatg aagagctgat gcaccatgtg ccggggattt tcggggaaga
    13021 gaaacacacg tcccgaagcc agaactatac gtacatcccc accatcaccg tactggaaag
    13081 tctgcagcgg gaaggcttcc agcccttttt cgcctgccag acccgtgtgc gcgacccggg
    13141 ccgccgggga tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga
    13201 acatgtccct gaaattattc tgctcaactc ccatgacggt acctccagct accagatgct
    13261 gccgggttac ttcagattcg tctgccagaa cgggtgtgtc tgtggtcagt ctctggggga
    13321 agtgcgtgtt ccgcaccggg gaaacgtggt ggagaaagtt attgaagggg cttacgaagt
    13381 ggtgggcgtg tttgaccgga ttgaggagaa acgtgatgcc atgcagtcgc tggtcctgcc
    13441 gccaccggca cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca
    13501 tcagcccgtc accaccgccg acattctgac gccacgacgc cgggaggatt acggtaagga
    13561 cctgtggagt acttatcaga ccatccagga gaatatgctg aaaggcggga tttccggccg
    13621 cagtgcaaaa ggaaaacgta tccacacccg tgccattcac aacatcgaca ccgatattaa
    13681 gctcaaccgc gcattgtggg tgatggcaga aacgctgctg gagagcctgc gctga
//
LOCUS       DAJTRN010000099        13653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRN010000099 DAJTRN010000000
VERSION     DAJTRN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            122..4240
                     /gene="pic"
                     /locus_tag="OO328_003155"
     CDS             122..4240
                     /gene="pic"
                     /locus_tag="OO328_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5931624.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGDTVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4391..4633)
                     /locus_tag="OO328_003156"
                     /pseudo
     CDS             complement(4391..4633)
                     /locus_tag="OO328_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(4672..5622)
                     /locus_tag="OO328_003157"
                     /pseudo
     CDS             complement(4672..5622)
                     /locus_tag="OO328_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5673..6248)
                     /locus_tag="OO328_003158"
                     /pseudo
     CDS             complement(5673..6248)
                     /locus_tag="OO328_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6252..6652
                     /locus_tag="OO328_003159"
                     /pseudo
     CDS             6252..6652
                     /locus_tag="OO328_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7055..7636)
                     /locus_tag="OO328_003160"
     CDS             complement(7055..7636)
                     /locus_tag="OO328_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931625.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(7683..11540)
                     /gene="sigA"
                     /locus_tag="OO328_003161"
     CDS             complement(7683..11540)
                     /gene="sigA"
                     /locus_tag="OO328_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5931626.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(11901..12905)
                     /locus_tag="OO328_003162"
                     /pseudo
     CDS             complement(11901..12905)
                     /locus_tag="OO328_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            12890..13267
                     /locus_tag="OO328_003163"
                     /pseudo
     CDS             12890..13267
                     /locus_tag="OO328_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13306..>13653)
                     /locus_tag="OO328_003164"
     CDS             complement(13306..>13653)
                     /locus_tag="OO328_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5931627.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         3412 a         3208 c         3329 g         3704 t
ORIGIN      
        1 ttcacaaaaa cacattaatg cagtaactat attttccttt ctggtgataa cgtcgggtta
       61 tcattagctt cttcagctat tttactttta tatcccttgt aaacatcatg gagaatccat
      121 agtgaataaa gtttattctc ttaaatattg ccccgtcacc ggggggctta ttgctgtctc
      181 tgaacttgcc cgcagggtaa taaaaaagac atgccgaaga ttaacgcata ttcttctggc
      241 tggcattcca gcaatctgtc tgtgttactc tcagatatcc caggcgggta ttgtccgttc
      301 cgatattgcc tatcagattt atcgtgattt cgccgaaaac aaagggcttt ttgtacctgg
      361 tgccaatgat attccggtat atgataagga cggaaaactt gtgggaagac tgggtaaagc
      421 cccaatggcc gatttcagca gtgtgagctc aaatggcgtt gctacgcttg tatcgcctca
      481 gtatatcgtc agcgtaaagc ataacggagg atatcggagt gtgagctttg gtaatgggaa
      541 aaatacatat tcccttgttg accgtaataa ccacccttct attgacttcc atgctccacg
      601 tctgaataaa ctggttacag aagttattcc ctcagcggta acatcagaag gaaccaaagc
      661 caatgcttat aaatacactg aacgttacac cgctttttat cgggtgggta gtggtacgca
      721 gtacactaag gacaaggacg gaaatttagt taaggttgcc ggtggatatg cttttaaaac
      781 aggaggaacc acaggagttc ctctgatatc tgatgcaaca atagtctcta atcccgggca
      841 aacttataat cctgtaaacg gccctttacc tgactatgga gcccctgggg atagtggttc
      901 tcctttgttt gcttatgata aacaacaaaa aaaatgggtt attgttgctg tattaagagc
      961 atatgcaggt attaatggtg ctacgaactg gtggaatgtc ataccaacag attatctgaa
     1021 ccaggttatg caggacgatt tcgatgcccc cgtagacttt gtttccggac tgggccccct
     1081 gaactggaca tacgacaaaa catcaggcac aggtaccctg agccagggca gtaaaaactg
     1141 gaccatgcac gggcagaaag acaatgacct caatgccggt aaaaatctgg tattcagcgg
     1201 gcagaatggt gcaattatcc tgaaagacag tgtgactcag ggtgccggtt atctcgaatt
     1261 taaagacagt tacaccgtat ctgctgaatc cggaaaaaca tggacgggtg ccggcattat
     1321 tactgacaag gggacgaatg taacctggaa ggtcaacggc gttgccggtg acaacttgca
     1381 taagctgggg gaaggaaccc tgaccataaa cggaacaggt gtaaacccgg gaggactgaa
     1441 aacgggagac ggtatcgttg tacttaacca gcaggcagac actgcaggta atatccaggc
     1501 cttcagttca gtgaacctcg ccagcggacg tccgaccgtg gtgctcgggg atgcccgtca
     1561 ggtcaatccg gataacattt catggggata ccggggaggt aagcttgacc ttaatggtaa
     1621 tgccgttacc ttcacccgac tgcaggctgc tgattacggg gcggtgatta caaataatgc
     1681 acagcaaaaa tcccagcttt tactggatct taaggctcag gatacaaatg tcagtgaacc
     1741 gacgattgga aatatatccc cctttggtgg taccggcact ccaggaaacc tgtacagcat
     1801 gatactcaac agccagaccc gcttctatat tctgaaatct gccagctatg gtaacactct
     1861 gtgggggaac agcctgaatg atccggctca gtgggagttt gttggcatga acaaaaacaa
     1921 agcagttcag acagtaaaag ataggatcct ggccgggcgg gcaaaacaac ccgttatctt
     1981 tcatggtcag ctgaccggga atatggatgt cgccattcca caggtgccgg ggggaagaaa
     2041 ggtaatcttt gatggtagcg tgaacctgcc ggaaggtacc ctgagtcagg acagtggcac
     2101 cctgatattc cagggacatc cggttatcca tgcctccatc agtggcagtg caccggtcag
     2161 cctgaaccag aaagactggg aaaaccgtca gtttacaatg aaaacactgt cgctgaaaga
     2221 cgctgacttc catctttcac gtaacgcctc gctgaacagt gacattaagt cggataacag
     2281 ccatatcaca ctgggaagtg acagggcatt tgtggataaa aatgacggaa caggaaatta
     2341 tgtcattccg gaggaaggta cctctgtccc ggacaccgtg aatgacagga gccagtatga
     2401 agggaatatt acgctgaacc ataactcagc cctggatatc ggcagcaggt tcaccggggg
     2461 gattgacgct tatgacagtg ccgtcagcat cacctctccg gacgtcctgt tgacagcccc
     2521 gggtgctttt gccggcagtt cactgacagt gcatgatggc ggtcatctta cagcactgaa
     2581 cggtcttttc agcgacgggc atattcaggc cggtaagaac ggcaaaatca ccctgagcgg
     2641 tacaccggtt aaagatacgg ctaatcagta tgcccctgct gtatatctga cggacggata
     2701 tgacctgacc ggcgataacg cagcactgga aattacccgt ggagcacatg cttccggtga
     2761 tattcatgcc tctgcggcat caacagttac catcgggtct gacacgccgg cagaactggc
     2821 ttctgcggaa acggctgcat cggcgtttgc cggcagtctt cttgagggct ataacgcagc
     2881 attcaatggt gccataaccg gtggcagggc tgatgtcagt atgcataatg cactgtggac
     2941 tctgggtggg gactctgcca tccacagtct taccgtcaga aacagccgta ttagttctga
     3001 aggagaccgt acattccgta ccctgacggt gaataaactg gatgcaacag gcagtgattt
     3061 tgttttgcgt acggacctga aaaatgccga taaaattaat gtgactgaaa aagccactgg
     3121 ttcagataac agcctgaacg tcagctttat gaataatcct gctcagggac aggccctgaa
     3181 tattcctctg gtcacggcac cggcgggaac ttcagcagag atgtttaagg ccggcacccg
     3241 ggtgacaggt ttcagtcggg tgaccccaac cctgcatgtt gataccagtg gtggcaatac
     3301 gaagtggata ctggatggtt ttaaagcgga ggctgataaa gccgctgccg cgaaggctga
     3361 cagtttcatg aatgccgggt ataaaaactt catgacggaa gttaacaatc tgaacaaacg
     3421 tatgggtgac ctgcgtgaca caaacggtga tgccggtgcc tgggcgcgca tcatgagtgg
     3481 tgccggttct gcagacggtg gttacagtga taattacacc catgttcagg tcggctttga
     3541 caaaaaacat gaactggacg gtgtggacct gtttaccggt gtcacgatga cctataccga
     3601 cagcagtgca gacagccatg cattcagcgg aaagacgaaa tcggtggggg gcggtctgta
     3661 tgcttcagca ttgtttgagt ccggtgccta tatcgatttg attggtaaat atattcacca
     3721 tgacaatgat tacacaggta actttgctag cctgggaacg aaacactaca acacccattc
     3781 ctggtatgcc ggtgctgaaa cgggttaccg ctatcacctg acagaggaca cgttcattga
     3841 gccgcaggct gaactggttt acggcgccgt gtccgggaaa acattccgct ggaaagacgg
     3901 tgatatggac ctgagcatga agaacaggga cttcagtccg ctggttggaa gaacaggggt
     3961 tgaactgggc aagaccttca gtggtaagga ctggagtgtg acggcccgtg ccggaaccag
     4021 ctggcagttt gacctgctga ataatggaga taccgtactg cgtgatgcgt ccggggagaa
     4081 acggataaaa ggagagaagg acagccggat gctgtttaat gttggtatga atgcgcagat
     4141 aaaggacaat atgcgctttg gtctggagtt tgagaagtca gcctttggta aatataacgt
     4201 ggataatgcg gtaaacgcga atttccggta tatgttctga tgtgggggta aagggctgcc
     4261 ggaaggcagc ctttctcaga gactcttaaa atataaaact gaaggtcttc ggtaaggggg
     4321 agtgtattag acgtcaacta gttttgccga ctggcgcgag cctgtttaag attctgtgta
     4381 aatgcctttc tcagaagtga ccgtccaggc ggtcaccgaa ctcgataata aagcggctca
     4441 ttgccattcg ccagtccttc aacggcatcg tccatttctg ggacgcagac tggattgcca
     4501 gccacaccac ttttttcacc gagtcgtctg tcgggaacac tttacgcttt ttgatcgcat
     4561 ggcggatcac gctgtttagc gactcgatgg cattcgtcgt atagatcact ttgcggatgt
     4621 ccgttggata agcggtaagc gtacagcgag ggccgtattg acggggatgt gttattcagc
     4681 tggcagtgct atgcgccacg gaagcagttc gctgacccgg ttgaccggcc agtctgctat
     4741 gacgccaagc acatggcgaa ggtagctttc tggatccacg tcattcagtt tgcacgtccc
     4801 gatcaggctg tacagtagcg cgccccgctc accaccatgg tcagagccga agaacaggaa
     4861 gtttttacga cccagactga ccgcccgcag ggcattttca gcgatgttgt tgtcgatttc
     4921 cacccagcca ttactgcagt acacgttcag ggccttccac tgtttcagta ggtatgcgaa
     4981 cgcttttgcc gtatccgagt gacgcgacag tgttttcatc tgttgctgta tccggtcata
     5041 cagtgactgc atcagtggcg cggctcttgc ttttcttgcc gccagacgct gttctgctga
     5101 acatccccgg acttctgcct cgatggtata cagttcaccg atacgctgca gggcttccgt
     5161 ggtgatgtcg gttggcactc ttgcatgcac atcgtggatt tttctccggg catgggccat
     5221 acacgcagct tccgttattc tgccggattc gtataacgcc cggtaaccac cgtaagcatc
     5281 tgcctgaagc acaccgctgt aaccggctag gtgattctgt ggatggatac ctttccggtc
     5341 cgggctgtac gcgaaccaga ccgccggggg catctgtgaa ccggcgttac ggtcatcacg
     5401 gacatagacc cacagccggg cagttcttgt tttaccactg cccggctcct ggaccggaac
     5461 ggggatatca tccgcatgaa ctttaccggg catcagtaca tactggcgca ggacgtcata
     5521 cagcggctcc agcagttcag caacggcacc ggtccagcga cccagtgtgg cacggctcag
     5581 ctccacgccc tgacgacggt atatttctga ctggcggtat aactgtcaac gacggatgaa
     5641 aagtggctct tccgcaacct gggtggagat tttcatgcca gtggactcat taccctctgc
     5701 ctgagtagtt caaatcctgc tcgaccatac atctggcgtt tcagcatctt cttcaggcga
     5761 tttacatgac cttcgacaac accattactc cagcgactac tgattgcctc acatatagct
     5821 gcagcatcag cttccatccc cgccgccacg cgccgaaatt ctgctgaacc gctttcgtgg
     5881 actcgagtga accaactgct tagctgtgat ttattttggg ttttcagaat acggtagaac
     5941 tcgagtgcca gttgctgcgc tattttcagc tcaggttctt tttcacacat cagactaata
     6001 aatcgggaag cataattttc ttcatccctg gttattctcc agggcatcaa ccagcggctt
     6061 acccgggaga ctgaaggaag tctgacggca gtagtaaccg gtgggttcca gcctttacgc
     6121 catttggcaa ccgcatccct gacgatggtt tcactgcctg taaatccccg ggccaccatt
     6181 tcccgccata tccggctggc gttataatta ccactttctc gttgctcttt taaccactcc
     6241 ctccatggtg tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc tggttgcgga
     6301 aaagatgaag acccagtcgc gacactcaga gttggcgaag gcgttcgcgt acgcacttaa
     6361 ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg acaacaacat
     6421 cgctgaaaat gccttgcggg cggtcagtct gggtcgtaaa aacttcctgt tcttcggctc
     6481 tgaccatggt ggtgagcggg gagcgctact gtacagcctg atcgggacgt gcaaactgaa
     6541 tgacgtggat ccagaaagct accttcgcca tgtgcttggc gtcatagcag actggccggt
     6601 caaccgggtc agcgaactgc ttccgtggcg catagcactg ccagctgaat aacacatccc
     6661 cgtcaatacg gccctcgctg tacgcttact catcacctgc tattaatccc cgagtcagta
     6721 cttcgcggcc tgacgcgatc accctactca gtacttcaga gcccgcctcc ggtacctgcc
     6781 tggcgatcgc ccctgtgcaa caaaataaat caaccgttat caaaccacga tcgttcgtat
     6841 gcgccgccga tgacgcgcgc ctgaaaaacg cgtcaatatg agttccccgg aagcttacga
     6901 taatacgctt atgaacatta caacacgctc atgcggaacc tggatggggg aaggaacggt
     6961 gaagctgtta gcgaatgacc ggcgtttata caaacgccgg tcatgatgaa tgatatttca
     7021 gaatatacct cgggctaaaa taccgggaca gagatcaggc gtgattaatc tgcagcattt
     7081 ttaataacag aagtactgag cttactttac ctttgatgat gtaatagtta tttccgtcgg
     7141 tactcatttt tccctgaaat aatgtcacgg atgtttcttc ctgctgatcc tgcaggcgca
     7201 gtcgtaaggt catattttta atgagctgag atccgggtgg caaggggtat ttgctgtcag
     7261 atactgggcg actgagtctg atgtggcgcg tatacagccc gttttcgtac agggtggcct
     7321 ctgttatggt gtcagtgacc agcatgtggg agacaatgct gtattctttt ttaaagcacc
     7381 caccagaaac ggtgattgta atgataatgg tcttctcgat cgagatgtaa atacccgttt
     7441 cgttatactg tccgtatcgg aaccatgaga actcagttgc attccagcca tgctccagaa
     7501 tatgtgcatt tttttgcctg atagtttctt tttttattcg ctcatgttta aggcataaca
     7561 ggcagataaa taatgtcaga aaaagaaagc taataatgag agcaagagcg atatagtctg
     7621 tcacagggta atacatattc tctccggtag aagaagggcc gcaaacgcgg cccgggctgt
     7681 tatcagaaag agtaacggaa gttggcgttg atcgcgttat ccacgttgta tttaccaaat
     7741 gccgatttct caaactcaag accgaagcga agattatcgc gaatttcggc gttgagacca
     7801 acattcatga gaatacgacc gtctttttca cctttgatac gtttctcacc ggacgcatca
     7861 cgcagtacgg tttcaccgtt ggcaaacagg tcaaactggt agccaaggcc ggcgcgggct
     7921 gtgactttcc agtccttacc ggagaaggat ttacccacat caacaccggt acgcccaatc
     7981 agcggattaa agtccttatc cttcatggtc acgctcattc cgcgatcctg ccagtcgaac
     8041 cgtttacctg atacggcccc ataaaccagt tctgcctgcg gctcaatcca ggagtcttct
     8101 gttacatgat agcggtagcc tgcttcagca cccacataca aggaatgaga actgtagtct
     8161 tttgttccga gtccagcaaa ggtcgccgag tactcattat catggtgaac atacttacta
     8221 atcaggtcga tataggcacc agagtcaaat atagcggaag catacagacc tgccccgacg
     8281 gacttcgtct tgccactgaa tgcattactg ctggcatgac tgtcggtata cgtcatagtc
     8341 agaccagtga aaaggtcaag tccatccagc tcatgttttt tatccacacc aatctgcaca
     8401 tgggtgtagt tgtcactgta tccaccagaa gctgaacctg ctccgctcat gatgcgggcc
     8461 catgcaccgg cctcgccatt ggtgtcacgc agatccccca ttcgtttatt caggttgttg
     8521 acttctgtca ggaaggcttt gtaattaccg gacatatacg attttgctgc accggaagat
     8581 gctgtatttt cagccacctt atatccggtc agggtccacg tggtatgttc ctgatcctct
     8641 ttcgtgctga tcacaggaga tatgttgctg aaacctatgg cctgagtact ggctttaaat
     8701 atctctttat tgcttccttt tggggctgtt atcaggtcaa cgtttaatcc tgagttatta
     8761 tttcccactt tctgtatatc gaccgacaga ctgttattgc ttccggttaa ctgattttta
     8821 acaatgagtt tatccgattg atttacagac tggctcagat cagcccgcat aatcacagca
     8881 gtattagtgg catccagtgt gtttactgtc acagtatgga aacccgaatt ctctatgtta
     8941 ctaaacaaca aaagactgtc gcgggttttt aatgattgca gggctgactg tccgtttact
     9001 ctccataagg tgttatacat gctggctgag gctgcaggag catcaatacc tccggtataa
     9061 gaagtatcaa agccacccag caaacctgta gccagcggtg tgtgtgtggt tttgtccagg
     9121 ttatcttctt tatactggtc aacaccaaac agaatagatg cttttttgtc tgattttata
     9181 ttaccattga cccaggcata attttgagcc tttaacgtgg caccatcttc tgtcatatta
     9241 tatccatctg acatatagat tgccgggctg tatttattat tttctgtagg ggtgcctgtt
     9301 aatgtcaggg ttccggccag ttcaatagct ttatctacat acatttttcc ctgggcggta
     9361 acagtagctc ctttgttgat aatcaggtct gtattaatta aggataatgc accagtcata
     9421 gtaacagttg atttatcacc aacagttatt ttgctgtttt gagcactcag actgccgttg
     9481 aaactacttt cgcctgctga atccccggag cgaagctgtt gtctgaatcc aaaaccttcg
     9541 ccagtaatgt ttcttcctga gaacatgtca atgtatgcag ttttatttcc cagaacaatt
     9601 tgagagtttt tagcctctat gtgtcctttt acattagcat cccggcctat actcagggtt
     9661 gcgttttcta aatttaattt ccggaaatta aatttacgac tttcccagtc gggctgggaa
     9721 aaagaggcta tctgattgtt ggacttatat tccgtattgt aacgtttatt tacagtgctt
     9781 tcctggtcct ttatggcagc agaatagtct ttttgacata aaaaaggaat tggacagttg
     9841 ttattgcctt ctttaaatat ggcatgttct gtcgcatgtc cttgtaatgt taactgtgca
     9901 ttccggacag agatatcgtt tttgatatcg actgagccat caaagatcag attggcatta
     9961 ttgttatgtt gctgggtagt attgataata tgatttatgc ttatattacc tgatacattc
    10021 ccatggtaca tatatgcatc agtattagta agtgataatg ttgattgctt cacgtttgag
    10081 ttcgttacgg ttgttcccgc atctgttgct gcgattttct gaaatgactg gtcatagccg
    10141 tttagatcca gtgttccgcc atttctggta aaatagattt cgccattacc gctttcgctt
    10201 aaagcgtctt tggcatttat ttttaccgta cctttacctc cggccatgta aattttattg
    10261 aaggcttttt cagcactaag gatgacagtc ccattaccta ttttaaggtt atttccctgt
    10321 gctattttta catccagagt accactacct attttatgca gattatctcc ggcaacgccc
    10381 ttaacattcc attcaacggt ggtattatta tcaatatcta tcccggcacc tttataagac
    10441 ttatcttttc cgtacacttt atatgtctgg ttcttatcga atataagccc gccaatgcca
    10501 agatcggtat cctgcttgag cgttaatacc cctcctcctg agacagacag gtccttatct
    10561 tttataatcg atgataactc ttgtttttta ccatccagct gcatagtgtt tgtgaaattc
    10621 ttttttaaat tgtttaccag aatattatta aataacgagt agtttgtcca ggctatattg
    10681 gtactactga taaaaccaac tgtactggta acaccatgga aaacccattt ttgttgttct
    10741 ttatcatata gataagaacc agaaccacta tcgccaaaaa ttggtgagtt tttaaaaggg
    10801 gttctgttgg tataaatgta actcttgttt ttccagtcca gattaaaaag acttgcgctt
    10861 aacaagtcgg gattatagtt ttgtccaaac agatattgtt tgccgtttaa tatcgttgag
    10921 gttgttccgg cacctgctct gaagccaatt atttgttcct taccgttgta gtttacgcca
    10981 tatcttttca tcgcatcttc gggagaaagt gagaagtcgg cactctccgt aacccctgtt
    11041 gtttccacga cgaatttatt cagacgatga accgaaaagt cgccagatga tactctgtta
    11101 cttgctttat atgctgtctg atcccatgac tgtgttgtta ttgcatgatg ctgtgtaccg
    11161 ttatgagtcg cagtaacact gtacgcacct ccaattgatg ttgttgcacc tttgttggaa
    11221 acagcagaaa aatcaggtac aggtagttct ggaaaatgaa atatctttcc atctttcatc
    11281 attaattgaa catttgttgc acccggtctg aactctcctc tgttttgagc aaggtcaaga
    11341 taatcgcggg tccatatatt atttatatcc agcattgctg caaatgctgt ttcgccataa
    11401 cttccatata ttgaggataa aataataccg aggataactt tctttcttga tgtaccgaca
    11461 ctaactttcc gggtcagttc agaaacagca actaatccac ctgtaatatg actatatttc
    11521 agtgaataaa ttttattcat acgtgaactc cgatatttct gagttgtggt tatgggatag
    11581 catttaatta caacggggaa ctatcagtca tggtacgtaa ctgataaggg tataatgata
    11641 atcactgtca gttatatttg tatgacagat gtaacaaaat gaaaaaaaag ataagcacaa
    11701 gtgattgatt ggttagatat tgatcacttg gtgtttgtat tttgaatttt gcgatcaaaa
    11761 tttgatcttt gttgtttccg gtcaatcctt tacaggtttt ctgtattatc cgggggagtt
    11821 aagtctgtcg atgtccgaca gacttttgat gatggaaaca acatgcagcg ataatgtgca
    11881 tgctgttttg caggtggaac tcagaaggtg aactggaact gtgcgaccgc accgtaagtg
    11941 ttgtccgtgc cgatcaggcc tgcaacatca aggtgaacca catcaaacgg tgagatcccc
    12001 accccggccg tgaatgcact gccgtcgtta ccggccagat tctgacggta accggcgcgc
    12061 agctgtgccc atttccaggc attaaattct gtgccaatgg cggcaaactg acggttgctg
    12121 tcggaggtga aaccgctggc cggggtcaga tccacatcaa atgcagtggt gaacatcgcg
    12181 ttgtgccagg agacaccggc cgtcacctgc gggcggatcc tgaaggtttc cgtgataccg
    12241 ttcacttctt ttgtctcaat actacgggag atcaggttct gtgcgaccag tcccagcgtc
    12301 cagttgtcat ccaggtccgt actggcaccg atgtcggcgt tgatgccatt tttcgtgttg
    12361 tggtagcggt cgcctttgaa ggcgctgctg tcataatttc tgaccagcac gttgtagttg
    12421 aacaggtcga cgcgctggaa tttggggggg aagcccagag accatttctg cccggccgtt
    12481 tccagttccg tagccagagc aatacccaca tcagtgatca gtgccgtgcg ggcacaggcc
    12541 tgtgatgtca ggcggctttt gtccacctcc tgcccggtgc tgtttgccac ctcttccagg
    12601 taagtgaggt cagcatcaga gactttaccg tcgacactga cggtgccgtg tgatttaacc
    12661 atcagtgcgg cagagaggcg atcaccggga agggccgcca cagcagaaac gcccagctgt
    12721 gcggcggcat gtgtgttacg gaagtcccgg aggtgttctt tcaggcgtgc ggcagcctca
    12781 ggaacaccgt ggttactttc gatggcgcgg tcgaaggcct tccactcatc actgatccgg
    12841 tcggcgttgt cggtgatatt atccggattg gagagctgcg cgccgacgga cggaagcagg
    12901 atgctgggct catgcgcgac gcaaaatcca cgatgtatat atcagtacta aaagcgcgac
    12961 ggcggaagaa gctctgaaac taatcggtga actgtacgcc atagagcacg atatacgcgg
    13021 gttaccggtg tctgaacgcc tggcggtcag gcaaatgcag agtagaccgc tcctgacttc
    13081 cctgtataag ctgatgcagg agaaagaaca cacgttatcg aagaaatgtc gtctgggaga
    13141 tgcgttccgg tatatcagga agcactgggc tgcgttgtgc aacttctgtg atgatggcct
    13201 ggctgaggta gataataatg cagcagaaag agcgcttcgt gcagtctgtc tcgggaagaa
    13261 aactttttcg aggatcactc attccgtgtc tgactgcttc agggtttaat tttgcttctg
    13321 ctgactgaat gatgattggg cagcaagtgc aatatcccac ataaatccag tcaactcacg
    13381 agccaccgcc gtaatggcta cttgtgattt tttccctttt gctgtcagag ttcggtaacg
    13441 agaacacaat cgttgttgag ctttccatgc aatatctttg gaatattgtg ttacagagtc
    13501 cggtcggtaa tatataagcc atgctccaac ttttgcaggt gtacgataag accaggcggc
    13561 ttcgtaaagc agacgtctca gttcgctgtt tccaactttt gttattcctc tgggacgaat
    13621 actgtttcca ctggaatatt cacctggcac cag
//
LOCUS       DAJTRN010000100        13089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRN010000100 DAJTRN010000000
VERSION     DAJTRN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..883)
                     /locus_tag="OO328_003165"
     CDS             complement(<1..883)
                     /locus_tag="OO328_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5931628.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVK"
     gene            1124..1924
                     /locus_tag="OO328_003166"
     CDS             1124..1924
                     /locus_tag="OO328_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5931629.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2002..2772
                     /locus_tag="OO328_003167"
     CDS             2002..2772
                     /locus_tag="OO328_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5931630.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2820..4166)
                     /gene="mltD"
                     /locus_tag="OO328_003168"
     CDS             complement(2820..4166)
                     /gene="mltD"
                     /locus_tag="OO328_003168"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5931631.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4238..4993)
                     /gene="gloB"
                     /locus_tag="OO328_003169"
     CDS             complement(4238..4993)
                     /gene="gloB"
                     /locus_tag="OO328_003169"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5931632.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5027..5749
                     /locus_tag="OO328_003170"
     CDS             5027..5749
                     /locus_tag="OO328_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5931633.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5746..6213)
                     /gene="rnhA"
                     /locus_tag="OO328_003171"
     CDS             complement(5746..6213)
                     /gene="rnhA"
                     /locus_tag="OO328_003171"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5931634.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6278..7009
                     /gene="dnaQ"
                     /locus_tag="OO328_003172"
     CDS             6278..7009
                     /gene="dnaQ"
                     /locus_tag="OO328_003172"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5931635.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7142..7218
                     /locus_tag="OO328_003173"
     tRNA            7142..7218
                     /locus_tag="OO328_003173"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7176..7178,aa:Asp,seq:gtc)
     gene            complement(7349..8395)
                     /locus_tag="OO328_003174"
     CDS             complement(7349..8395)
                     /locus_tag="OO328_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5931636.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8409..8968)
                     /gene="tssG"
                     /locus_tag="OO328_003175"
                     /pseudo
     CDS             complement(8409..8968)
                     /gene="tssG"
                     /locus_tag="OO328_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8965..10848)
                     /gene="tssF"
                     /locus_tag="OO328_003176"
     CDS             complement(8965..10848)
                     /gene="tssF"
                     /locus_tag="OO328_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5931637.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10864..11358)
                     /gene="tssE"
                     /locus_tag="OO328_003177"
     CDS             complement(10864..11358)
                     /gene="tssE"
                     /locus_tag="OO328_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5931638.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11355..12185)
                     /locus_tag="OO328_003178"
                     /pseudo
     CDS             complement(11355..12185)
                     /locus_tag="OO328_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12172..13086)
                     /gene="tagK"
                     /locus_tag="OO328_003179"
                     /pseudo
     CDS             complement(12172..13086)
                     /gene="tagK"
                     /locus_tag="OO328_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3006 a         3392 c         3479 g         3212 t
ORIGIN      
        1 tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt accgcacggt
       61 cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct gccattaact
      121 ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa cacgcaatcc
      181 cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac aaaccgtact
      241 tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg gaagcgggtt
      301 cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc ttcccgtagc
      361 gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac ggcctggcac
      421 gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt ctttcgatca
      481 aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag cgttcacgga
      541 aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca tcgatccgta
      601 acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct gccatctcct
      661 gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg ctaagttgcc
      721 gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc accgcccggc
      781 ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg ccgctttcga
      841 cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct cctgttgcaa
      901 aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt gatatgtcat
      961 ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact tgcttacata
     1021 acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt agaaccctcc
     1081 cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa aaaacaccta
     1141 tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg ggtcttttgc
     1201 gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc gtattcggat
     1261 cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat taaagaacta
     1321 cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag agttagtaca
     1381 gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc tgccacaaca
     1441 tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc cgttacgtga
     1501 acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc cattacctga
     1561 cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg gcgtggatgt
     1621 ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg gcccggtcat
     1681 tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat atcgctttgc
     1741 gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc gggcgtttgg
     1801 ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt ctgtattggt
     1861 tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca agcctgataa
     1921 ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc ttttatactc
     1981 aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt agaaaagcag
     2041 tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg acgagattta
     2101 cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga tatgggctgc
     2161 ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt ggcgtatgac
     2221 ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca actgaaaaat
     2281 atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc atttgatatt
     2341 gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact gcgtgaagtg
     2401 aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc tccgggtcat
     2461 ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc tcacgtacga
     2521 aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat agttgataat
     2581 ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat gcgtacgcca
     2641 gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga ggtgagaacg
     2701 tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt agaagcacat
     2761 aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta tctggtttgt
     2821 caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc tggttgcaga
     2881 ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg gcgtttagca
     2941 atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct gttgtttgcc
     3001 agtcgctgtg caccaatcgt caaactttgg cctggcttca gcttagatcc gcgcagtttg
     3061 ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct tgaaagcgtg
     3121 tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgttggc aaccagcgtc
     3181 gactgtacag cagcaatttc gcctgaagcg agagattcac gcagttgatc tgcatgcttc
     3241 tttggcacca tcacgtactg cggcccactt gcgcccagcg tggagccttt cacgccagcg
     3301 ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt cgccatttca
     3361 accgggctac tcaggtgcac acgcgccaga gcacggcttt catcggtcgt tggcagacgt
     3421 acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt aggcacgtac
     3481 tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc acgcgctttg
     3541 ttcgttttaa ttgccttcat gacccgacct tcgccgctgt tataagccgc tacggtcaga
     3601 agccagtcgt cgtcaaacat tttgttcaga cgctgcatca tattcagcgc ggcagttgtt
     3661 gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc ataattgcgc
     3721 cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt tgcgtgagga
     3781 tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt acgtttttta
     3841 acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc gtggagatag
     3901 ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc cggaattccc
     3961 atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat agacgtccca
     4021 tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc agctgcagaa
     4081 aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag caggacagag
     4141 gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa gacgaccgat
     4201 cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct ttctttgacc
     4261 ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta attccattaa
     4321 ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt ttcagaatta
     4381 cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa taatcattta
     4441 tggacaaatc gtgcggaaaa atactcaaag caaacttcat atttgataac gtatattcat
     4501 gagcacaaca taccaatgta tcgtcaggta acgcacttag ctttttaagt gattgataca
     4561 tttgtaaagc tgtcccttca aacaaccgac cacacccacc agaaaacagc gtgtcgccgc
     4621 aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc ggtgtggtaa
     4681 ttacactaaa ttcatgccct aaaacgaagg cagtttcgcc atctttgact acctgtgttg
     4741 ttcccttatc ttgtgtctct tgtggaccat acactacaat ttgcggaaac ttttccacca
     4801 gttcttttac gccgccaacg tgatcgtggt gatggtgggt tagaaatatg gcctccggtt
     4861 gccagttatt ggcggtaatg gcgtttaata ctggctctgc gtctccggga tcgacaatca
     4921 ggcagcgacc tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag gcgggaatac
     4981 tgttaagatt catagattac ctctcagtgt aaaacggaag gttgtgatga aaccggcaag
     5041 agtccctcaa actgtcgtgg ctcctgattg ctggggcgat ttgccctggg gagagcttta
     5101 tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg gttttcatct
     5161 gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg tttctcatca
     5221 agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac ttcatcttcc
     5281 ttttgccgat aaatccgttg atatttgtct actggcacat accttgccgt ggtgcaccga
     5341 tccgcatcgt ttattacgtg aagccgatcg ggtactgatt gatgatggct ggctggtgat
     5401 tagtggcttc aaccccatca gtttaatggg attacgcaaa cttgtgccgg tattgcgcaa
     5461 aacctcgccc tataatagcc ggatgtttac tctgatgcgg caactggact ggctctcttt
     5521 gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga acaaacacgg
     5581 aggaaaacta ttgaatgcgc atattcctgc gcttggttgc ttacaactta ttgttgcccg
     5641 gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca agccacgaat
     5701 tcgccaggcg gttggagcca cccggcaatg tcgtaaacca caggcttaaa cttcgacttg
     5761 gtagcccgta tcttccagtg tgggattcat cgccgcggca cgagccagtt catcacagcg
     5821 ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt gatgctgccc
     5881 caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt tgtctgctgt
     5941 tttccagcca cgttttttcc agttatggat ccactgggtg ataccctggc ggacatactg
     6001 gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg cgacaatagc
     6061 ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa aggttttctc
     6121 gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac ccagacacga
     6181 accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg taattgaatc
     6241 gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa ttacacgcca
     6301 gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcacact atgaaggcca
     6361 caagatcatt gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg gcaataactt
     6421 ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg tacatggtat
     6481 tgctgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg agtttatgga
     6541 ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatag gctttatgga
     6601 ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct gtaaggtcac
     6661 cgatagcctt gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc tcgatgcgct
     6721 atgtgcccgc tacgaaatag ataacagtaa acgaacgctg cacggggcat tactcgatgc
     6781 ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga tggcttttgc
     6841 gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca ttgtacgtca
     6901 ggcaagtaag ttacgcgttg tttttgcgac agatgaagag ctggcagcac atgaagcccg
     6961 tctcgatctg gtgcagaaaa aaggcggaag ctgcctctgg cgggcataaa tacatgtaaa
     7021 aggcgctaaa aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa gatgagaaaa
     7081 atcgttgacg aaggtcgagg caatccgtaa tattcgcctc gttcccaacg gaacacaacg
     7141 cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc gcgggttcga
     7201 gtcccgtccg ttccgccaat attcagaaag cccgaatcag taatgattcg ggctttcgtc
     7261 gttttggtga agtgagaatt tttcactccg aagtaaaacg gatatgattt aaaaaatggc
     7321 cagacattat aaattctggc cacttctgtt actccagcgc atcccatttc tccagcagtt
     7381 ctactggaat ttcatttatt acctccgaga cgcgttttcc gaccaggcgt tctgcctggc
     7441 gcagcaaagg aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga cggatgacac
     7501 gcaggcgttc cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg ccggtcatcg
     7561 gtgatatttc ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc atctgttcag
     7621 tatgaacgat ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg atctccggcg
     7681 tggtgctctg cactgctccc ggtaacaggg ccagcagttg cgtcagacgg gagaagtccg
     7741 gtgcctggtc attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg gtctctgctg
     7801 cctggcgaaa ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc agttgctggc
     7861 gtaccgattc tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa cgctccacat
     7921 cccgcacctg aaggcgcatg gcagcattat ttgacagtgt aatgccacga atatccgcca
     7981 ttacaccttc gtggtccagc aacgccgcca gcgcgttact gcgtgccacc gcagcatctt
     8041 cggcggtgat atcttcagtc gcgagcagtt gaggatggat ggcgtccggg tacgtggagc
     8101 acaacgtttc cagcagcgtg agcgcctccg acagtcccct ggcaccggcc tgctggatac
     8161 ggcagcgcag caggacaacc agaatgcgga tatctttgct gcgcatcagc agacgatggg
     8221 cgtcccgctc aagctccgcc cagttaatgt tttccggcgt actgacaaaa tcgccatatt
     8281 gcgcttcttc acgcggtgcg gcgcgggtga acagcagcag gtattccgga tcgtactcca
     8341 gatccgggcc gcaggggcat tccgccgaaa ttgggtcaga caggaatatt tccatttaat
     8401 tatcctcagt gcgccagcgt ggtggccaga ccgtccggat tgctggactc acgcaccaga
     8461 tgggccgatg ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct ctcccgcaaa
     8521 tcctgcccgc tgaggctggc gacataaaag gcaaagcggt catgttgcgg tttatccaga
     8581 ccgcccccag cggactgggc gctgcgccat gcctggtaaa actgcgtcag ccagcggtga
     8641 tgaaaaagat cagaaaaacg ggtcagcgtc ggctttcagt ccggttacgc gccagttcgg
     8701 tgtagtgcag cggcagtgga ccgttcggcc cccacagcac gaggctgtac agactgatgt
     8761 gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag gtcagcgcgg
     8821 gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcca ggtgttgccc gccggaacgg
     8881 tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaacgc cagggggggg
     8941 cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc tgacaggcca
     9001 ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg tattaatgga
     9061 aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac tgatgccgga
     9121 aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga ccagtagtcc
     9181 ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc tgcgcacctg
     9241 gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc gcaacgcctg
     9301 gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata actggcggat
     9361 caaccgccat gccatctcac gttcagccat cgggggctgc ggcgagcgtg gcgggcggat
     9421 aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc ggccattgcg
     9481 ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga tgtggcgcag
     9541 attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt ccgagcccgt
     9601 atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg gctcccggcg
     9661 cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt agagcgggcg
     9721 aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga ccgagaacac
     9781 ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg tgctgtgcgt
     9841 gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac agaacaggct
     9901 gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt tcagcaggat
     9961 aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc ctgtcggggt
    10021 gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat gaccgtgaaa
    10081 cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt acataacggg
    10141 ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag ccaggggcgc
    10201 gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct cgccgcagag
    10261 ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac caaaggtgcg
    10321 cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc ggtgcagggc
    10381 aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg gccacagcgt
    10441 tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa tggcggagaa
    10501 aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt ccgtatcggg
    10561 atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc tgaccacttc
    10621 cagcaggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg tgcgggcggt
    10681 caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat ccgttccctg
    10741 catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc ccgtttcccg
    10801 cagatagctc agttcgcggt tgtagtattc cagtagccgt ggatccatcg gtctctctct
    10861 gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta cccggaatgc
    10921 agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg tgttatggcc
    10981 actctggcgg cgctcatcct gaagtgggat gatttgcaga gtggacgctt tcagccgggg
    11041 ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact tgtgctcata
    11101 cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg ccgcgtacgg
    11161 ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa atgacaaatc
    11221 ccggcggatg atctccttaa tctgaaccgg actgacgctg ataccgtggt cgcgtttctg
    11281 gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat tgcctggacg
    11341 ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca ggtggacaaa
    11401 tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag cgcctgtacc
    11461 agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag ggagtcggca
    11521 gccttttcag agccgctgta gcggggaaac agctaggcac tgtgtgtatc gccattgttc
    11581 agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg cggcgatttc
    11641 agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat cagttcgagt
    11701 accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc attccactgc
    11761 ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc ttcctgcgct
    11821 tcaccacggg cgttacaggc gagggcggtc agcagatcct ccatccaggc gggcggcggg
    11881 agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat ctcacagcgg
    11941 atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg ggcgcatagc
    12001 tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg aaacaggctg
    12061 tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat ctgaatgccc
    12121 tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc gttattgttt
    12181 gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggctgt caatgccaat
    12241 gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg tttccggggc
    12301 gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag gccagatagt
    12361 ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta acccaccagt
    12421 gaccatatcc tgcaatgtct cggcttcggt gaccaggtca cgcaatgaat ccagatcctg
    12481 cgaggagggc tcttcgttaa aaggaaaagg atcagcatgg cgattaagat cttgttcggg
    12541 agataacaac gcctcgcggt attcctgata aagtcgggtc agggcatttc ctttttccgc
    12601 accttctccg gtggaggagg ctccatgaac aggaatgata tcgaatggat tttgtgatgt
    12661 cagatgctgc tgcctgaacc aatccagatc cagatattcg gttattgctt ccgacgtgat
    12721 ggcagtctgc gatatcgttt ttactgtgct caggttcgga gatgatggat tgccatgtac
    12781 aaggcactat gaagataacc cccattctat caggtcgcct tcattcagac gcatacggtg
    12841 atgaggttcc acaatctggt cgtttaccgt acagcaatat tcggtagact gattgataat
    12901 ccaccatgct tcttcgtggc gggtcagagt gagggcaatc tttacatcat cattcacctt
    12961 aaaaacagga atccagggag aggtaacggt gaagataatt tgtgttcctt cagtcgcgta
    13021 aattgtgtta tcggcgatcc cgtgcgcctg tattttttgg agtccccacg taatatttgg
    13081 tttcattgt
//
LOCUS       DAJTRN010000101        12902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRN010000101 DAJTRN010000000
VERSION     DAJTRN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO328_003180"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO328_003180"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5931639.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO328_003181"
     CDS             complement(1806..3224)
                     /locus_tag="OO328_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5931640.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO328_003182"
     CDS             complement(3378..3722)
                     /locus_tag="OO328_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5931641.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO328_003183"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO328_003183"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5931642.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO328_003184"
     CDS             complement(4712..5647)
                     /locus_tag="OO328_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5931643.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO328_003185"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO328_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5931644.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO328_003186"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO328_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5931645.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO328_003187"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO328_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5931646.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9080..9514
                     /gene="marR"
                     /locus_tag="OO328_003188"
     CDS             9080..9514
                     /gene="marR"
                     /locus_tag="OO328_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5931647.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9534..9917
                     /gene="marA"
                     /locus_tag="OO328_003189"
     CDS             9534..9917
                     /gene="marA"
                     /locus_tag="OO328_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5931648.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9949..10167
                     /gene="marB"
                     /locus_tag="OO328_003190"
     CDS             9949..10167
                     /gene="marB"
                     /locus_tag="OO328_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5931649.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10198..11097)
                     /gene="eamA"
                     /locus_tag="OO328_003191"
     CDS             complement(10198..11097)
                     /gene="eamA"
                     /locus_tag="OO328_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5931650.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11361..12479
                     /gene="ydeE"
                     /locus_tag="OO328_003192"
     CDS             11361..12479
                     /gene="ydeE"
                     /locus_tag="OO328_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5931651.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12606..12701
                     /gene="mgtS"
                     /locus_tag="OO328_003193"
     CDS             12606..12701
                     /gene="mgtS"
                     /locus_tag="OO328_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5931652.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3399 a         3298 c         2949 g         3256 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcatttga cttatacttg cctgggcaat attaccccct acaactaatt
     9061 acttgccaag gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt
     9121 gggtcgctta atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc
     9181 tccgctggat attaccgcgg cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg
     9241 tattactccg gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac tgacccgtat
     9301 gctggatcgc ctggtctgta aaggctgggt tgaaaggttg ccgaacccga atgataagcg
     9361 cggcgtactg gtaaaactta ccaccagcgg cgcggcaata tgcgaacaat gccatcaatt
     9421 agttggccag gacctgcatc aagaattaac aaaaaacctg acggcggacg aagttgcaac
     9481 acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg acgatgtcca
     9541 gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg
     9601 aatcgccact gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc
     9661 aacggatgtt taaaaaagaa accggtcatt cattaggcca atacatccgc agccgtaaga
     9721 tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac
     9781 gatatggctt cgagtcgcaa caaactctga cccgaacctt caaaaattac tttgatgttc
     9841 cgccgcataa ataccggatg accaatatgc agggcgaatc gcgcttttta catccattaa
     9901 atcattacaa cagctagttg ataacgtgac aacgtcactg aggcaatcat gaaaccactt
     9961 tcatccgcaa tagcagctgc gcttattctc ttttccgcgc agagcgttgc ggaacaaacc
    10021 acgcagccag ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa
    10081 ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg
    10141 ccctattata atcaacacgc tatgtagttt gttctggccc cgacatctcg gggcttatta
    10201 acttcccacc tttaccgctt tacgccaccg caagccaaat acattgatat acagcccggt
    10261 cataatgagc accgcaccta aaaattgcag acccgttaag cgttcatcca acaatagtgc
    10321 cgcacttgcc agtccgacta cgggcaccag taacgataac ggtgcaaccc gccaggtttc
    10381 atagcgtccc agtaacgtcc cccagatccc ataaccaaca attgtcgcca caaacgccag
    10441 atacatcagc gacaagatgg tggtcatatc aatagtaacc agactgtgaa tcatggatgc
    10501 ggaaccatcg agaatcagcg agacaacaaa gaagggaatg attgggatta aagcgctcca
    10561 gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt tattgaagat
    10621 gttgccacac gcccaactaa atgctgcagc cagggtcaac ataaagccga gcatcgccac
    10681 atgctgaccg ttcagactat cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat
    10741 ccccgccaat tgtttgccat gcagtcgctc cccgaaagta aacgcgccaa gcacgatagt
    10801 aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac caaagttaat
    10861 ggcgcagaac agaaaggcaa actgcgcaaa actgatggtt aatccatacc ccaagagcaa
    10921 attcagtggt actttcggtc gtgcaacaaa aaagatggcc ggaaaagcga ccagcataaa
    10981 gcgcaaaccg gccagcatca gcggtggcat gttatgaagc cccactttga tgaccacaaa
    11041 atttagcccc catacgacca ctaccagtag cgccaacacc ccatcttttc gcgacattct
    11101 accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac ttttctttaa
    11161 cagttgattc gttagtcgcc ggttacgacg gcattaatgc gcaaataagt cgctatactt
    11221 cggatttttg ccatgcgtcc gatggttaat aacaacgacg aggcaagaag ggcgctggta
    11281 gagcgtcgta gggataagtt catttgtctg tccggcagtg cgtttcgtta tgttttattt
    11341 tagagatgta aagaaatagc atggctacgc tgccatttat gaccatttac ttgagtcgcc
    11401 agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc actattggcg
    11461 tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa cgctatatgt
    11521 tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg aataacgtga
    11581 cgctggttgt gctctttttt gccctcatta actgcgccta ttctgttttt gctaccgtgc
    11641 tgaaagcctg gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca
    11701 actacaccat gctaaacatt ggctggacca tcggtccgcc gctcggcacg ctgttggtga
    11761 tgcagagcat caatctgccc ttttggctgg cagctatctg ttccgcgttt cccatgcttt
    11821 tcattcaaat atgggtaaag cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct
    11881 ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc tctggttttc
    11941 tggcttcttt tgtaagcggc gcatttgctt catgcatttc acaatatgtg atggtgattg
    12001 ctgatgggga ttttgccgaa aaggtggtcg cggttgttct tccggtgaat gctgccatgg
    12061 tggttacgtt gcaatattcc gtgggccgcc gacttaaccc agctaacatc cgcgcgctga
    12121 tgacagcagg caccctctgt ttcgtcatcg gactggtcgg ttttattttt tccggcaaca
    12181 acctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc atttatgcgc
    12241 cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc agctattttt
    12301 ccgcccagtc tttaggctgg cttggtgccg cgattaaccc attagtgagt ggcgtagtgc
    12361 taaccagcct gccgccttcc tcgctgtttg tcatcttagc gttggtgatc attgctgcgt
    12421 gggtgctgat gttaaaaggg attcgtgcaa gaccgtgggg gcagcccgcg ctttgttgat
    12481 ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttatctc tgctaatatc
    12541 tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat aaacggaggc
    12601 aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt ttattttctg
    12661 gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag ataatccctc
    12721 acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg acggcgaaaa
    12781 aaaccgccag taaaccggcg gtgaatgctt gcatggatag atttgtgttt tgcttttacg
    12841 ctaacaggca ttttcctgca ctgataacgg atcgttgaca cagtagcatc agttttctca
    12901 at
//
LOCUS       DAJTRN010000102        12654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRN010000102 DAJTRN010000000
VERSION     DAJTRN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..403)
                     /locus_tag="OO328_003194"
     CDS             complement(<1..403)
                     /locus_tag="OO328_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR5931653.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYG"
     gene            complement(663..1283)
                     /gene="sodA"
                     /locus_tag="OO328_003195"
     CDS             complement(663..1283)
                     /gene="sodA"
                     /locus_tag="OO328_003195"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5931654.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1568..2602
                     /gene="rhaT"
                     /locus_tag="OO328_003196"
                     /pseudo
     CDS             1568..2602
                     /gene="rhaT"
                     /locus_tag="OO328_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2599..3465)
                     /gene="rhaR"
                     /locus_tag="OO328_003197"
     CDS             complement(2599..3465)
                     /gene="rhaR"
                     /locus_tag="OO328_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5931655.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(3521..4357)
                     /gene="rhaS"
                     /locus_tag="OO328_003198"
     CDS             complement(3521..4357)
                     /gene="rhaS"
                     /locus_tag="OO328_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5931656.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4645..6114
                     /gene="rhaB"
                     /locus_tag="OO328_003199"
     CDS             4645..6114
                     /gene="rhaB"
                     /locus_tag="OO328_003199"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5931657.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6111..7370
                     /gene="rhaA"
                     /locus_tag="OO328_003200"
     CDS             6111..7370
                     /gene="rhaA"
                     /locus_tag="OO328_003200"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5931658.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            7612..8436
                     /gene="rhaD"
                     /locus_tag="OO328_003201"
                     /pseudo
     CDS             7612..8436
                     /gene="rhaD"
                     /locus_tag="OO328_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            8446..8760
                     /gene="rhaM"
                     /locus_tag="OO328_003202"
     CDS             8446..8760
                     /gene="rhaM"
                     /locus_tag="OO328_003202"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5931659.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9061..9507
                     /locus_tag="OO328_003203"
     CDS             9061..9507
                     /locus_tag="OO328_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5931660.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            9518..10969
                     /locus_tag="OO328_003204"
     CDS             9518..10969
                     /locus_tag="OO328_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5931661.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            10959..12029
                     /locus_tag="OO328_003205"
     CDS             10959..12029
                     /locus_tag="OO328_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5931662.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12029..>12654
                     /locus_tag="OO328_003206"
     CDS             12029..>12654
                     /locus_tag="OO328_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5931663.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         2991 a         3289 c         3354 g         3020 t
ORIGIN      
        1 taccgtactg ctgcatgatg gaagcgtaaa gtccgccgtt ggtcatatcc atcgccgcca
       61 ccagcgccag cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa
      121 tgcgcgaggc aattgccgca accacccacg cgacggcaat tttagttacc accagcgtac
      181 cggatttacg cagtaccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa
      241 accacaccgc cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat
      301 atttccccgc ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc gggacgagca
      361 tcatcccccc cgggattttc tcaatcgagc gttttatctg catttccatc acctgtcggc
      421 atatttttaa gggctgatgc gtaattcctc aatggagcat cagtgttgat ggaaacgaag
      481 atagacattc atttaatcaa taaaagtgat caaaatcaca tcaattgaaa cgctgtttta
      541 tttttatagg accattgttt ttattttgga attgatcaca aaaaaaccac cgccgttggc
      601 gatggttcat tttttaagct gatatgcggc ctatacgcct cattgcagca ggcggcaaat
      661 gattattttt tcgccgcaaa acgtgccgct gcttcgtccc agttcaccac gttccagaac
      721 tctttgatgt agtccggacg gcggttctgg aatttcaggt agtaagcatg ttcccacaca
      781 tccaggccca gaatcgggaa gccggaagcg ccagaaatag cttcacccat cagcggagaa
      841 tcctggttag cagtagaaac caccgccagt ttatcgcctt tcagcaccag ccatgcccag
      901 ccggaaccaa agcgggaagc tgccgctttt tcaaattctg ctttgaagtt atcaacggag
      961 ccgaagtcac gttcgatagc cgctttcagg tcaccctgca gggtggtgcc ttttttcaga
     1021 cctttccaga acaggctgtg gttagcgtga ccgccagcgt tgttgcgcag tacggttttc
     1081 ttgtctgctg gcagctggtc cagtttggtg atcagctctt caaccggcag gttggcaaat
     1141 tctggcaggc tttccagcgc cgcgttggcg ttgtttacgt aggtctgatg gtgtttggtg
     1201 tggtggattt ccatggtctg cttatcgaag tgcggttcca gggcatcgta agcatacggc
     1261 agggatggca gggtatagct catattcatc tccagtattg tcgggcggcc gattgttaat
     1321 gccgcgtaag cagttggttc attatagtta attaaatgat attgaaaatg attatcaatg
     1381 ccgtactttt cgtaagggta tggttttgca ggaaaatgcc cgagatgtga agcaaatcac
     1441 ccacttaatg ccgtgattgc cagtaaatcg acaacggcgg caacaggcga aaggttaatc
     1501 gacagcacga tttttacact catctcgtcg gagatgtgaa gcgacgaaaa atgatgagga
     1561 taagaagatg agtaacgcga ttacgatggg gatattttgg catttgatcg gcgcggccag
     1621 tgcagcctgt ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg
     1681 gtcagtcggt gggattgttt cgtggattat tctgccgtgg gccatcagcg ccctgttact
     1741 accggatttc tgggcgtatt acagctcgtt tagtctctct acgctgctgc ctgtttttct
     1801 gttcggcgct atgtggggga tcggtaatat caactatggc ctgaccatgc gttatctcgg
     1861 catgtcgatg ggaattggca tcgccattgg cattacgttg attgtcggta cgctgatgac
     1921 gccaattatc aacggcaatt tcgatgtgct gattaacacc gaaggcggac gcatgacgtt
     1981 actcggcgtt ctggtggcgc tgattggcgt agggattgta actcgagccg ggcagttgaa
     2041 agagcgcaag atgggcatta aagccgaaga gttcaatctg aaaaaagggc tggtgctggc
     2101 ggtgatgtgc ggcattttct ctgccgggat gtcctttgcg atgaacgccg caaaaccgat
     2161 gcatgaagcc gctgccgcac ttggcgtcga tccactgtat gttgctctgc caagctatgt
     2221 tgtcatcatg ggcggcggcg cgatcattaa cctcggtttc tgttttattc gtctggcaaa
     2281 agtgaaggat ttgtcgctaa aagccgactt ctcgctggca aaaccgctga tcattcacaa
     2341 tgtgttactc tcggcactgg gcgggttgat gtggtatctg caattctttt tctatgccta
     2401 gggccacgcc cgcattccgg cgcagtatga ctacatcagt tggatgctgc atatgagttt
     2461 ctatgtattg tgcggcggta tcgtcgggct ggtgctgaaa gagtggaaca atacaggacg
     2521 ccgtccggta acggtgttga gcctcggttg tgtggtgatt attgtcgccg ctaacatcgt
     2581 cggcatcggc atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt
     2641 catcccggtt tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc
     2701 ggtcgaaata tcactgatta acaggcggct atgctggaga agatattgcg catgacacac
     2761 tctgacctgc cgcagatatt gattgatggt cattccagtc tgctggcgaa attgctgacg
     2821 caaaacgcgc tcactgcacg atgcctcatc acaaaattta tccagcgcaa agggactttt
     2881 caggctagcc gccagccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg
     2941 caacgaatca ctggtgtaac gatggcgatt cagcaacatc accaactgcc cgaacagcaa
     3001 ctcagccatt tcgttagcaa acggcacatg ctgactactt tcatgctcaa gctgaccgat
     3061 aacctgccgc gcctgcgcca tccccacgct acctaagcgc cagtgtggtt gccctgcgct
     3121 ggcgctaaat cccggaatcg ctccctgcca gtcaagattc agcttcagcc gctccgggca
     3181 ataaataata ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc
     3241 atgaatataa aagagatcgc cacgggtaat gcgataagga cgatcgttga gtacatgcag
     3301 accattaccg cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cggcaaagac
     3361 atcttgcgga taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc
     3421 tttgagaagt tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt
     3481 attcgcaata tggcgtacaa atacgttgag aagattcgcg ttattgcaga aagccatccc
     3541 gtccctggcg aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt
     3601 ggttactgtc gctgaatcca cagcgatagg cgatgtcagt gacgctggcc tcgctgtggc
     3661 gtagtagatg tcgggctttc atcagtcgca ggcggttcag gtatcgctga ggcgtcagtc
     3721 ccgtttgctg cttaagctgc cgatgtagcg tacgcagtga aagagaaaat tgatccgcca
     3781 cggcatccca attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga
     3841 gacgtgatgc gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta
     3901 attgcataaa caagatctcg cgactggcgg tcgagggtaa atcattttcc ccttcctgct
     3961 gttccatctg tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta acgcgccagt
     4021 gagacggata ctgcccatcc agctcttgtg gcagcaactg attcagcccg gcgagaaact
     4081 gaaatcgatc cggcgagcga tacagcacat tggtcagaca cagattatcg gtatgttcat
     4141 acagatgccg atcatgatcg cgtacgaaac agaccgtgcc accggtgatg gtatagggct
     4201 gcccattaaa cacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat
     4261 gatgatgttc aggaaaatcc gcctgcggga gccggggttc tatcgccacg gacgcgttac
     4321 aagacggaaa aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac
     4381 acggcgaaat agtaatcacg aggtcaggtt cttaccttaa attttcgacg gaaaaccacg
     4441 taaaaaacgt cgatttttca agatacagcg tgaattttca ggaaatgcgg tgagcatcac
     4501 atcaccacaa ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaatg
     4561 gcccattttc ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta gactggtcgt
     4621 aatgaaattc agcaggatca cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc
     4681 gcatccagtg ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg
     4741 cgcgaaatcc atcgttttaa caatgggctg catagccaaa acggttatgt cacctgggat
     4801 gtggatagcc tggaaagtgc cattcgcctt ggattaaaca aggtgtgcga ggaagggatt
     4861 cgtatcgata gcattgggat tgatacctgg ggcgtggact ttgtgctgct cgaccaacag
     4921 ggtcagcgtg tgggcctgcc cgttgcttat cgcgatagcc gcaccaatgg cctgatgccg
     4981 caggcacaac agcaactcgg caaacgcgat atttatcagc gtagcggcat ccagtttcta
     5041 cccttcaata cgctttatca gttgcgtgcg ctgacggagc aacaacctga acttattcca
     5101 cacattgctc acgctctgct gatgccggat tacttcagtt atcgcctgac cggcaagatg
     5161 aactgggaat ataccaatgc caccaccaca caactggtca atatcaatag cgacgactgg
     5221 gacgagtcgc tactggcgtg gagcggggcc aacaaagcct ggtttggtcg cccgacgcat
     5281 ccgggtaatg tcattgggca ctggatttgc ccgcagggta atgagattcc ggtggtcgcc
     5341 gttgccagcc atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc
     5401 gcttatctct cttctggcac ctggtcattg atgggcttcg aaagccagac gccatttacc
     5461 aatgacacgg cgctggcagc caacatcacc aatgaaggcg gggcggaagg tcgctatcgg
     5521 gtgctgaaaa atattatggg cttatggctg cttcagcgag tgcttcagga acggcaaatc
     5581 aacgatctcc cggcgcttat cgccgcgaca caggcacttc cggcctgccg cttcatcatc
     5641 aatcccaatg acgatcgctt tattaatcct gacgagatgt gcagcgaaat tcaggctgcg
     5701 tgtcgggaaa cggcgcaacc gatcccggga agtgatgctg aactggcgcg ctgtattttc
     5761 gacagtctgg cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct gcgcggtgaa
     5821 gatttctcgc aactgcatat tgtcggcggc ggctgccaga acacgctgct caaccagcta
     5881 tgcgccgatg cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc aacgctcggc
     5941 aatatcggca tcgagttaat gacgctggat gaactcaaca atgtggatga tttccgtcag
     6001 gtcgtcagca ccaccgcgaa tctgaccacc tttaccccta atcctgacag tgaaattgcc
     6061 cactatgtgg cgcagattca ctctacacga cagacaaagg agctttgcgc atgaccactc
     6121 aactggaaca ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg
     6181 aggaggcgct gcgccaactt gatcgtttac ccgtttcaat gcactgctgg cagggcgatg
     6241 atgtttccgg ttttgaaaac ccggaaggtt cgctgaccgg ggggattcag gccacaggca
     6301 attatccggg caaagcgcgt aatgccagtg agctacgtgc cgatctggaa caggctatgc
     6361 ggctgattcc ggggccgaaa cggcttaatt tacatgccat ctatctggaa tcagatacgc
     6421 cagtctcgcg cgaccagatc aaaccagagc acttcaaaaa ctgggttgaa tgggcgaaag
     6481 ccaatcagct cggtctggat tttaacccct cctgcttttc gcatccgcta agcgccgatg
     6541 gctttacgct ctcgcatccc gacgacagca ttcgccagtt ctggattgat cactgcaaag
     6601 ccagccgtcg cgtttcggcc tattttggcg agcaactcgg cacaccatcg gtgatgaaca
     6661 tctggatccc ggatggtatg aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc
     6721 tgctggcagc actggatgag gtgatcagcg agaagctaaa ccctgcgcac catatcgacg
     6781 ccgttgagag caaattgttt ggcattggcg cagagagcta cacggttggc tccaatgagt
     6841 tttacatggg gtatgccacc agccgccaga ctgcgctgtg cctggacgcc gggcacttcc
     6901 acccgactga agtgatttcc gacaagattt ccgccgccat gctgtatgta ccgcagttgc
     6961 tgctgcacgt cagccgtccg gttcgctggg acagcgatca cgtagtgctg ctggatgatg
     7021 aaacccaggc aattgccagt gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg
     7081 gccttgactt cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt ggtacacgca
     7141 atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgctgagctg cgcaagctgg
     7201 aagcggcggg cgattacact gcgcgtctgg cactgctgga agagcagaaa tcgttgccgc
     7261 ggcaggcggt ctgggaaatg tattgccaac atcacgatac gccagcaggt agcgaatggc
     7321 tggagagcgt gcgggcttat gagaaagcga ttttgagcca gcgtgggtaa acactgccgg
     7381 atgcggcacg agcgccttat ccggcctacg ggtcgaatgt ccatgcgggc tgtcaccctc
     7441 accccggccc tctccctgaa agggcgaggg agaaaaacat agcctcgtgc ggcaacggag
     7501 ctgaacaccg ctccggacag tcccctcgcc cctacgggga gagggttagg gtgaggggaa
     7561 caagccgcac catgtctaaa caaggcaacc taatttacag gaacacagaa catgcaaaac
     7621 attacccagt cctggtttgt ccagggaatg atcaaagcca ccaccgatgc ctagctgaaa
     7681 ggctgggacg agcgcaacgg cggcaacctg acgctgcgcc tggatgacgc cgatatcgcg
     7741 ctttatcacg acaacttcca cccacaaccg cgctatattc cgctcagcca acccatgcct
     7801 ttactggcaa atacgccgtt tattgtcacc ggctccggca aattcttccg taacgtccag
     7861 ctcgatcctg cagctaactt aggcgtcgta aaagtcgaca gcgatggcgc gggctaccat
     7921 attctctggg ggttaaccaa cgaagccgtt ccaacttctg aacttccggc tcactttctc
     7981 tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat gcactgccac
     8041 gccaccaacc tgatcgccct cacctatgta ctggaaaacg acaccgcggt ctttactcgc
     8101 caactgtggg aaggcagcac cgaatgtctg gtagtattcc cggatggcgt tggcattctg
     8161 ccgtggatgg tgcccggcac ggacgaaatc ggccaggcga ccgcacaaga gatgcaaaaa
     8221 cattcgctgg tgttgtggcc cttccacggt gtgttcggtt ccggaccgac gctggatgaa
     8281 gcctttggtt taatcgacac cgcagaaaaa tcagcacaag tattagtgaa ggtttattcg
     8341 atgggcggca tgaaacagac catcagccgt gaagagttga tagcgctcgg caagcgcttc
     8401 ggcgttacac cactagccag tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc
     8461 tttgtcatgc aggtaaaccc cgacgcccac gaagagtatc agcgtcggca taatcccatc
     8521 tggccggaac tggaagcagt gttgaaatct cacggtgtgc ataactacgc catttacctc
     8581 gacaaagcgc gtaatctgct gtttgccatg gtagagattg aatctgaaga acggtggaat
     8641 gcggtagcca gtactgatgt ttgccaacgt tggtggaaat atatgaccga tgttatgccc
     8701 gctaacccag ataacagccc ggtgagtagc gagctgcaag aagtgtttta cctgccgtaa
     8761 ttgcccaatg tgccggagat gcctccggca caatatttta aatttcgatt tgaaaaatta
     8821 aacatattac atctcccggt aagttatatt tccctgatac attgtgagta aatcacaaaa
     8881 ataatgaata acccattaat gattcatgtg gtttatttaa ataaccatta tgtgcattac
     8941 tccgcaaatc tgacctttca ctctgtctca aattgtcatt gtttaccgac atatcgcctc
     9001 tcatcactta atcgctccac cattacaatt gattaaaata tattttatgg agtccgatta
     9061 atggcagctc ttactgcaag ctgtattgac ctgaatattc agggcaatgg cgcttattcc
     9121 gttctgaagc agttggcgac aatagcgtta caaaacggtt ttatcaccga ctcacaccag
     9181 ttcctgcaaa ccctgctgct gcgcgaaaaa atgcactcta ctggatttgg ttccggtgtc
     9241 gcagtgccgc acggtaaaag cgcctgcgtt aaacaaccgt tcgtattatt cgcccgcaaa
     9301 gcgcaggcta ttgactggaa agccagcgat ggcgaagacg tcaattgctg gatctgcctc
     9361 ggcgtgccgc aaagcggcga agaggatcag gtcaaaatca tcggcacact gtgtcgcaaa
     9421 attattcacc aggaatttat tcatcaactg caacagggcg ataccgacca ggtgctggcc
     9481 ttgttaaatc aaaccctcag ctcataagga agtggcgatg gagtcatcct tacgtattgt
     9541 cgcgatcacc aactgccccg ccgggatcgc tcacacctac atggtggcgg aagccctgga
     9601 acagaaagcc cgttctctcg gccataccat aaaagtggaa actcaagggt ccagtggcgt
     9661 tgaaaaccgc ttatccagcg aagagattgc cgctgccgat tacgtcattc ttgctaccgg
     9721 gcgtggcctg agcggtgatg atcgcgcgcg gtttgccggg aagaaagttt atgagattgc
     9781 catctccctg gcgttgaaaa atatcgacca gattttcagc gaattaccga caaactcgca
     9841 gctttttgcc gcagatagcg gcgtgaagct tggcaaacag gaagtgcaaa gcggcagcgt
     9901 gatgagccac ctgatggcgg gcgtctctgc ggcgctgccg tttgtcatcg gcggtggtat
     9961 cctggtggcg cttgccaaca tgctggtgca gttcggtttg ccgtataccg atatgtcgaa
    10021 aggcgcgccg tcgtttacct gggtagttga atccatcggt taccttggct tcacctttat
    10081 gatccccatc atgggcgctt atattgcctc gtcgattgcc gataaaccgg ctttcgctcc
    10141 ggcgtttctg gtttgctatc tggcgaacga caaagcgctg ctcggcaccc agtcgggtgc
    10201 gggattcctc ggcgcggtgg tgctggggct ggcgattggc tatttcgtct tctggttccg
    10261 caaagtgcgc cttggcaaag cattacaacc gctgctcggt tcaatgctga tcccgttcgt
    10321 tactctgctg gttttcggcg tgctcactta ctacgttatc gggccggtga tgtccgacct
    10381 catgggcggg ctgctccact tcctgaatac cattcctcca tcaatgaagt ttgcggcagc
    10441 gtttctggtc ggtgcgatgc tggcgttcga tatgggcggc cccatcaata aaaccgcctg
    10501 gttcttctgc ttctcactgc tggaaaaaca catttatgac tggtacgcca tcgtcggcgt
    10561 tgtcgcgttg atgccgcccg ttgcggcggg tcttgcgacg ttcattgcgc ccaaactgtt
    10621 tacccgacag gaaaaagagg ctgccagtag tgccattgtg gtcggtgcca cagtcgcaac
    10681 cgaaccggct ataccttacg ctctggcagc tccgttgccg atgattaccg ccaatacgct
    10741 ggcgggcggc attaccggcg tgctggtgat cgctttcgga atcaaacgtc tggcacctgg
    10801 tttgggtatc tttgacccgc tgatcggcct gatgtcgccg gtaggttcgt tttatctggt
    10861 gctggcgatt ggcctcgcgc tgaacatttc attcattatc gtcctgaaag gattgtggtt
    10921 acgccgcaaa gcgaaagccg cgcaacagga gcttgtccat gaacattgag ttactgcaac
    10981 agttgtgcga agccagcgcc gttagcggcg atgaacagga agttcgcgac atcctgataa
    11041 acacgctgga accttgcgtt aatgaaatca cctttgatgg tctgggcagc tttgttgccc
    11101 gtaagggaaa taaaggtcca aaagttgccg ttgtcgggca tatggatgaa gtcggcttta
    11161 tggtcaccca catcgacgag agcggttttc tgcgtttcac caccattggt ggctggtgga
    11221 atcagtcgat gctcaaccat cgggtaacca tacgcacaca caagggagtg aaaatccctg
    11281 gtgtgattgg tttcgtcgcg cctcatgcgt taacggaaaa gcaaaggcaa cagccgctgt
    11341 catttgatga gatgttcatt gatattggcg caaacagtcg cgatgaggtg gaaaaacgcg
    11401 gtgtggcgat gggcgatttt attagcccgg aagccaattt tgcctgctgg ggcgaagata
    11461 aagtggtcgg caaggcgctg gataaccgca tcggctgcgc aatgatggct gaactattgc
    11521 agacggtgaa taatcccgaa attacgctgt atggcgtcgg cagcgtggaa gaagaagttg
    11581 ggctacgcgg ggcacaaacc tcggcggaac acattaaacc ggatgtagtg atcgtgctgg
    11641 ataccgctgt cgcaggcgat gttccgggca ttgataacat taaatacccg ctgaaactgg
    11701 gccaggggcc ggggctgatg ctgtttgaca agcgctactt ccccaaccag aaactggtag
    11761 cagcgttaaa aaactgtgcc gcacataacg atttaccgct gcaattttcc accatgaaaa
    11821 ccggagcgac ggatggcggg cgctacaacg tgatgggcgg cgggcgtccg gttgttgcgc
    11881 tgtgcctgcc aacccgctat ctgcacgcta acagcggcat gatttcaaaa gccgattacg
    11941 atgctctgct cacgttgata cggggttttc tgacgacctt aaccgcggag aaagtcaacg
    12001 cgtttagcca gttccgtcag gtggattaat gctaaacgaa cgccagttaa agattgtcga
    12061 tctgctggag caacagccgc gcacgcctgg tgagctggcg caacagactg gcgtttcagg
    12121 caggaccatc ctgcgtgata ttgactatct caactttacc cttaacggca aagcccgcat
    12181 tttcgccagt ggcagtgcgg gctatcagct ggaaatcttc gagcgccgca gcttttttca
    12241 gttgctgcaa aagcacgata acgacgatcg gctgctggcg ctgttattac tgaatacttt
    12301 cactccccgt gcgcaactcg cctcggcgct taatttgcca gaaacgtggg tagcagagcg
    12361 tctgccccag ttaaaacagc gttatgaacg cacttgttgc ctggccagcc gccctggttt
    12421 gggccatttc attgatgaga cagaagagaa actcgttatc ttgctggcga acttgctgcg
    12481 caaagatccg tttttaattc cgctggcggg cataacacga gacaaccttc agcatttatc
    12541 cacggcctgc gacaaccaac accgctggcc gctcatacag ggtgattatc tctccagcct
    12601 gattctggcg atttacgccc tgcgtaatca actgaccgat gagtggccgc aata
//
LOCUS       DAJTRN010000103        12628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRN010000103 DAJTRN010000000
VERSION     DAJTRN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1028
                     /locus_tag="OO328_003207"
     CDS             <1..1028
                     /locus_tag="OO328_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5931664.1"
                     /translation="LVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWF
                     DEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDA
                     EHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIG
                     GMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHD
                     GNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNT
                     CEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPI
                     RPIARRT"
     gene            1029..3194
                     /locus_tag="OO328_003208"
     CDS             1029..3194
                     /locus_tag="OO328_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5931665.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3208..4119
                     /locus_tag="OO328_003209"
     CDS             3208..4119
                     /locus_tag="OO328_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5931666.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4119..5414
                     /locus_tag="OO328_003210"
     CDS             4119..5414
                     /locus_tag="OO328_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5931667.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5459..5689
                     /locus_tag="OO328_003211"
     CDS             5459..5689
                     /locus_tag="OO328_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931668.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5667..6167
                     /locus_tag="OO328_003212"
     CDS             5667..6167
                     /locus_tag="OO328_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5931669.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6167..7585
                     /locus_tag="OO328_003213"
     CDS             6167..7585
                     /locus_tag="OO328_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5931670.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7585..8433
                     /locus_tag="OO328_003214"
     CDS             7585..8433
                     /locus_tag="OO328_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931671.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8433..8888
                     /locus_tag="OO328_003215"
     CDS             8433..8888
                     /locus_tag="OO328_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5931672.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8891..9583
                     /locus_tag="OO328_003216"
     CDS             8891..9583
                     /locus_tag="OO328_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5931673.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9593..10924
                     /locus_tag="OO328_003217"
     CDS             9593..10924
                     /locus_tag="OO328_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5931674.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10925..>12628
                     /locus_tag="OO328_003218"
     CDS             10925..>12628
                     /locus_tag="OO328_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5931675.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DP"
BASE COUNT         3397 a         3125 c         3331 g         2775 t
ORIGIN      
        1 accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa gatggcattt
       61 caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt gacacaatcc
      121 acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt cttacccgta
      181 ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg atgtctgacg
      241 ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac atgaccatct
      301 atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca tatcccgagc
      361 atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga atcttccaga
      421 taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc tacgtaattg
      481 gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg tgggataagg
      541 aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca gccgttcagg
      601 cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct catgatggaa
      661 accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac gctggattta
      721 tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag ccaggcatca
      781 ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc tgtgagccat
      841 tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc aagcttaacg
      901 acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca aaaatgatgc
      961 gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc atcgcacgga
     1021 gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt gacgcagact
     1081 ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt agccgagtgt
     1141 cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga caattcgatg
     1201 ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct gtcgacgttc
     1261 tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg gggatgtatc
     1321 gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt cgtgagcaga
     1381 tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac gatccaacaa
     1441 gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac gtcatatggg
     1501 acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg attaacgcct
     1561 tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg gataccctgc
     1621 catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat gatgtcgtct
     1681 acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc taccgcgacc
     1741 cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc atcgacgacc
     1801 tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg cgtgtgtata
     1861 agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc ggagagcata
     1921 ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag tgctacgagg
     1981 gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg tcgttcaacg
     2041 ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag caaatcgaag
     2101 gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag aacaggactg
     2161 atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac cctgaagtgc
     2221 cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa gtggctagtc
     2281 ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc aatcaactga
     2341 acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc gcaatgcgac
     2401 gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct cgtcatgtaa
     2461 cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa gttgtcgatt
     2521 accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag tgctatacag
     2581 acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt caggagttgc
     2641 tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa tacttcacgc
     2701 tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa ctggtgatga
     2761 tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag gcacaacaac
     2821 agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg caaggtcagg
     2881 ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct gccaaggttg
     2941 aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat atggacctcg
     3001 acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag caacagcgca
     3061 gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag actcattcac
     3121 aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc ggagtagccg
     3181 agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca ggcttctgaa
     3241 gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt tgtcgataat
     3301 gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga cgatgagaaa
     3361 ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga acgcaaacgc
     3421 cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc ggagcacctg
     3481 cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc cgaagatgca
     3541 ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca ggcaaacagt
     3601 gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca gggtcgcaaa
     3661 actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa gcactacgag
     3721 gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt catgcaactg
     3781 gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc cgccgctctc
     3841 atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat ggacgggcaa
     3901 tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc tcgagccaag
     3961 cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt tgctgcaaat
     4021 atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt agagacgtac
     4081 cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat gaaggtcaac
     4141 tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg ccaatggcgt
     4201 caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc aacaccgtgt
     4261 ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact ggcaacgcaa
     4321 ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac gatttcttcg
     4381 agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc caggcatccg
     4441 ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact gaaatgggct
     4501 cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct ggctgggatt
     4561 ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac atgggcatca
     4621 gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta gatggtgaca
     4681 tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt cagattgctg
     4741 gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca accgcgactg
     4801 gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt gataccgatg
     4861 gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc accaccggat
     4921 ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag atggcgaaga
     4981 atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt actcacatcg
     5041 aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa gagaaggctt
     5101 acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg aacgtggcaa
     5161 caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct cagccgatcc
     5221 cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt ccaggtattg
     5281 gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt aagtgccgta
     5341 ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt ggtctgccta
     5401 accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg agacaagcat
     5461 gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg gctacgactt
     5521 tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt tttcacatcc
     5581 tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc gtaaaaagcc
     5641 tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat ttagttcttg
     5701 cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc gaaccgcagt
     5761 ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt ggcggtgatg
     5821 cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct ccggatccgg
     5881 gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac cttgcctgcc
     5941 gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact gccagatacg
     6001 ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct aaatgtaagt
     6061 ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag tggaatggtt
     6121 ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc ctattcagca
     6181 acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata tcgactatct
     6241 gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat atcttcgctc
     6301 attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg tcgagtacaa
     6361 catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag gagaaagtga
     6421 agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga catcacaggc
     6481 ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca aaaccgtctc
     6541 aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc gcgacattac
     6601 gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt ttatcactga
     6661 ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg agtctcagcc
     6721 tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg gttcatcgac
     6781 tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt atgttgcaca
     6841 accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc cattcgctga
     6901 ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata tcatcgactc
     6961 cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct cctacactgc
     7021 tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg agttgctgat
     7081 tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca atggcccaca
     7141 atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg acttcgttta
     7201 cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga aactgcaatt
     7261 cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc cactcttcaa
     7321 agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg ttgcgcagta
     7381 cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc gtgagcagat
     7441 gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc gagtagggcg
     7501 catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg tcactctgtc
     7561 tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc ggtaatcatc
     7621 caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca gtcgtatctg
     7681 ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc caacgaggcc
     7741 ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat tctcgctgac
     7801 catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga gcgaatcaga
     7861 caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga aagcgatatt
     7921 tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca tgaaacgcgc
     7981 atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta ctctggtgta
     8041 tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac gctcacaccg
     8101 tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt tgtgttcaac
     8161 gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag cggaaccgcc
     8221 aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt cagcagtcgt
     8281 aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt ggataaagac
     8341 ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc ggcgtttact
     8401 gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca acgcgaaaca
     8461 tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc aacaacggtg
     8521 atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac ggtcagtttg
     8581 gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac gccatgtacc
     8641 tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga tacattctga
     8701 ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga cacggtcaga
     8761 tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac ttcaaaggcg
     8821 tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc ctgaatcacg
     8881 ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg aggaacctta
     8941 ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt atgcagcaga
     9001 agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca tgaaaaacct
     9061 tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc tttccagttt
     9121 agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata aagaccttgc
     9181 agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac ttggttcgac
     9241 agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt ggttatcaaa
     9301 tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc aaggtcaggc
     9361 aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa gcgcagctct
     9421 tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg gtggcacgtc
     9481 tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc cttggggcgc
     9541 tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa tcatggctac
     9601 ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact caaacgctcc
     9661 gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg atattgagcg
     9721 ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta ttgcgggtgt
     9781 ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt acgctaatgc
     9841 ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc cagaccagat
     9901 tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt ctatcggcac
     9961 cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat catggctgca
    10021 aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg ctcagatgta
    10081 ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga acagtctcgg
    10141 gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta ccatgcgcgg
    10201 tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc gcggacaaga
    10261 tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa accgcattaa
    10321 caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc tgaagcgcca
    10381 ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc agcaagcgct
    10441 aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca ctctgaacga
    10501 tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa tccccaacgt
    10561 tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat cacaagcata
    10621 cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg agggcaaaaa
    10681 gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct ttcgtcgcaa
    10741 cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg gtaagtacgt
    10801 tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt acctgaagca
    10861 gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat ctttggggca
    10921 gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg gtgtgccaga
    10981 agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg cagtaagctc
    11041 cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata tgggttatac
    11101 cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct atctggcaaa
    11161 gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg acggtcccgg
    11221 caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc gcggttacgt
    11281 tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag tcgaacagcg
    11341 cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg cacaactgga
    11401 agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa ataatccatt
    11461 tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa gttctgtcgc
    11521 accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa cacgggaaga
    11581 accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg cagggcaaat
    11641 tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc agggtggcgc
    11701 aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg atgatgttta
    11761 tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa taggcggtta
    11821 tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg ctgacgcggc
    11881 gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta gcattgctac
    11941 gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata taattagtgg
    12001 caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac cattctccgg
    12061 tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag tagcacaagg
    12121 tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag tagcagcaca
    12181 aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc aggctgaagt
    12241 tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact tttccgggaa
    12301 cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg ctctacaggt
    12361 gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa ttgatcgcgt
    12421 ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg tcaatggaag
    12481 aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa aggcgatgcc
    12541 tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg tggcagaaga
    12601 tcttggcggg atgaaaaacc ttgaccct
//
LOCUS       DAJTRN010000104        12529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRN010000104 DAJTRN010000000
VERSION     DAJTRN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..572)
                     /gene="rutR"
                     /locus_tag="OO328_003219"
     CDS             complement(<1..572)
                     /gene="rutR"
                     /locus_tag="OO328_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5931676.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRD"
     gene            860..1951
                     /gene="rutA"
                     /locus_tag="OO328_003220"
     CDS             860..1951
                     /gene="rutA"
                     /locus_tag="OO328_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5931677.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            1951..2643
                     /gene="rutB"
                     /locus_tag="OO328_003221"
     CDS             1951..2643
                     /gene="rutB"
                     /locus_tag="OO328_003221"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5931678.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2655..3041
                     /gene="rutC"
                     /locus_tag="OO328_003222"
     CDS             2655..3041
                     /gene="rutC"
                     /locus_tag="OO328_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5931679.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3049..3849
                     /gene="rutD"
                     /locus_tag="OO328_003223"
     CDS             3049..3849
                     /gene="rutD"
                     /locus_tag="OO328_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5931680.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3859..4449
                     /locus_tag="OO328_003224"
     CDS             3859..4449
                     /locus_tag="OO328_003224"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5931681.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4460..4954
                     /gene="rutF"
                     /locus_tag="OO328_003225"
     CDS             4460..4954
                     /gene="rutF"
                     /locus_tag="OO328_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5931682.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            4975..6303
                     /gene="rutG"
                     /locus_tag="OO328_003226"
     CDS             4975..6303
                     /gene="rutG"
                     /locus_tag="OO328_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5931683.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6385..6492)
                     /locus_tag="OO328_003227"
     CDS             complement(6385..6492)
                     /locus_tag="OO328_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931684.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6865..7461
                     /gene="wrbA"
                     /locus_tag="OO328_003228"
     CDS             6865..7461
                     /gene="wrbA"
                     /locus_tag="OO328_003228"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5931685.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7482..7709
                     /locus_tag="OO328_003229"
     CDS             7482..7709
                     /locus_tag="OO328_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5931686.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7747..8988)
                     /gene="agp"
                     /locus_tag="OO328_003230"
     CDS             complement(7747..8988)
                     /gene="agp"
                     /locus_tag="OO328_003230"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5931687.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9281..10537)
                     /locus_tag="OO328_003231"
     CDS             complement(9281..10537)
                     /locus_tag="OO328_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5931688.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10798..11718
                     /gene="cbpA"
                     /locus_tag="OO328_003232"
     CDS             10798..11718
                     /gene="cbpA"
                     /locus_tag="OO328_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5931689.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11718..12023
                     /gene="cbpM"
                     /locus_tag="OO328_003233"
     CDS             11718..12023
                     /gene="cbpM"
                     /locus_tag="OO328_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5931690.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12116..>12529)
                     /gene="torD"
                     /locus_tag="OO328_003234"
     CDS             complement(12116..>12529)
                     /gene="torD"
                     /locus_tag="OO328_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5931691.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         2990 a         3198 c         3162 g         3179 t
ORIGIN      
        1 tcatcgcgca acgtcgcgcc agtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt
       61 tgagtggaag cccaaatcat aaaaatcaaa tgctgcggat caatcggcgc gagtttgccg
      121 cttttgaccc aaccggcaat cagcgccgat ttctcatcaa ttaatgcctt caaatcgccc
      181 gtcagttcat ccattaacaa cggcgcgcct gccagcatct ccatacagaa caggcgcgaa
      241 gcctgcggat aatcgcgtga gacttccagc ttcagacgga tgtactcttt tatcgccgcc
      301 agcggggcga aatcttcacg aaacgctttt aacggtgcca gccagatatc gagaatctgc
      361 cgcagcacgg caatatacag cgcctctttt gacgggaagt aatacagcag attggttttt
      421 gaaacacccg ccaactctgc gatctgctcc agccttgtgc cgtgaaaacc gaattgtgaa
      481 aaagtgtcca gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt
      541 ttacccgttg ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggtaac
      601 aggatagcaa aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acggatgcat
      661 gttttatgtg caactgtttt gaccgtttag tccacttttt accagatatt ttagataaca
      721 caatgctaac acattaataa tattcacttt ttaaaaactg gcatccgctt tgcgaacaag
      781 ccaatacgcg gctgaaaagg atatgcagga tgcagcgccc cgtctgactt tcactttacg
      841 agacgaagag aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg
      901 gctcatttcg acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccattgc
      961 gcaaaaagcg gagcattacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt
     1021 tggcggcaaa actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcgggtct
     1081 ggcagccgtg acctcgcgca ttcagattta cgccactgct gccaccttaa cgttacctcc
     1141 ggcaatcgtc gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt
     1201 caacctcgtg actggctggc aaaagcccga gtatgagcag atgggtatct ggcctggcga
     1261 tgactatttc tcccgtcgtt acgactatct caccgaatat gttcaggtgc tgcgcgacct
     1321 gtggggcacg gggaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt
     1381 cagtccgcaa ccgagtatcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat
     1441 ggcgttctcc gcccagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacacc
     1501 cacggctttc gccccgaccg ctgcgcggat gaaacaggcc acagagcaaa ccggacgcga
     1561 cgttggctct tatgtgttgt ttatggtgat tgccgatgaa accgacgatg ccactcgcgc
     1621 caaatgggaa cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca
     1681 aagtcagaaa gatacccgct ccggtactga caccaacgtt cgtcagatgg ccgatcccac
     1741 ttcggcggta aacatcaata tggggacgtt agtcggttct tacgccagtg tcgcgcgcat
     1801 gttagatgaa gtcgcaagcg tgcctggtgc agaaggcgtg ctgttgacct tcgatgattt
     1861 tctgtcggga atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca
     1921 tctctctgcg ctgactcagg aggtggcatg atgacaacct taaccgctcg accggaagcc
     1981 attaccttcg atccgcagca aagtgcgcag atcgtggtgg atatgcaaaa cgcttatgcc
     2041 acgccaggcg gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt
     2101 gccaacattc aaaccgctgt gaccgcagcg cgagcggcag ggatgttgat catctggttt
     2161 caaaatggct gggatgaaca gtatgtcgag gctggcggcc ccggctcacc gaattttcat
     2221 aaatcgaacg ccctgaaaac catgcgtaag cagccgcagc tgcaggggaa attgctggcg
     2281 aaaggctcct gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg
     2341 ctgccgaagc cgcgctacag cggtttcttc aatacgccgc tggacagcat tttacgcagc
     2401 cgtggaatac gccatctggt tttcaccggt atcgctacca acgtctgcgt cgaatcgacg
     2461 ctacgcgatg gcttttttct ggagcatttc ggcgtggtgc tggaagacgc aacacaccag
     2521 gcggggccgg aatttgcaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg
     2581 gtcagcgacg tcgaaacgtt ctgcgacgcg ctttctccca cgtcctttgc tcgtatcgct
     2641 taaggagttt agcgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg
     2701 cccccttcgt tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt
     2761 ttgatcaaca taataacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc
     2821 tggaaactat ccgcaaggtg atcgagacgg cgggtggcac gatggcggat gtgaccttca
     2881 acagcatctt tattaccgac tggaaaaatt acgccgcgat taacgaaatc tacgccgagt
     2941 tttttccggg tgataaaccg gcgcgattct acattcagtg cggactggta aaacctgacg
     3001 cgctggtgga aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca
     3061 ctctcatctc ccccttatgc tgatgcaccc gtagtggtgt tgatttcggg tcttggcggt
     3121 agcggcagtt actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt
     3181 tacgaccagc gcggcaccgg caataatccc gacacactgg cagaagatta cagtatcgcc
     3241 cagatggcag cggaactgca tcaggcgctg gtagccgcag ggattgagcg ttacgcggtg
     3301 gtcggccatg cgctcggtgc gctggtgggg atgcagctgg cgctggatta tcccgcgtcg
     3361 gtaactatgc tggtcagcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt
     3421 tttcaggttc gcgaacgatt actgtatagc ggcggcgcgc aagcatgggt ggaagcgcag
     3481 ccgttgttcc tctatcccgc cgactggatg gcggcccgcg cacctcgcct ggaggcagaa
     3541 gacgcgctgg cactggcgca ttttcagggc aaaaataatt tactacgtcg acttaacgcc
     3601 ctcaaacgcg ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc
     3661 tgcgccagtg atgatctgct ggtgccaaca gcatgttcca gtgaacttca tgccgccctg
     3721 cccgatagcc agaaaatggt gatgccctat ggcggacacg cctgcaacgt gaccgatccc
     3781 gaaacgttta atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc
     3841 gccctgtaag gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccctgttc
     3901 accgatgccc gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacag
     3961 gagctttatg ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacggatc
     4021 gtgtttatcc gcactgcaga aggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat
     4081 ctgcaaaaaa ccctgaccgc gcccgtcacc gctatcgtcg cctgggacag tgaattttat
     4141 gaacggttac cactactgtt tccccacggt gatgcccgca gttggtttac ctccagccca
     4201 caacttgccg aagaaacagc gtttcgcaac agttccatgc aggcggctta tctgatcgtc
     4261 gcttgccgtg cgctgggact ggataccggc ccgatgtcgg gttttgaccg tcaacacgtt
     4321 gacgacgcct tttttgcggg cagcacgctg aagagcaatc tgctgattaa tatcggctat
     4381 ggcgatagca gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg
     4441 ctgttgtaag gagcaaacta tgaacatagt tgatcaacaa acctttcgcg atgcgatgtc
     4501 ctgtatgggc gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt
     4561 caccgccagc gccgtctgca gcgtgaccga cacgccgccc acattactgg tgtgcctgaa
     4621 tcgtggggcg tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact
     4681 gagcgccggg caggagccgc tttcaaatct ttttggcggc aaaacgccca tggaacaccg
     4741 ctttgccgcc gcccgctggc agacgggtgt gaccggatgc ccgcaactgg aagaggctct
     4801 ggtttcgttt gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattctgtt
     4861 ttgcgccatc gaagcgattc atcgtcacac cacaccctac gggctggtgt ggttcgatcg
     4921 cagttatcac gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca
     4981 atgttcggtt ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg
     5041 ccggatgaac gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg
     5101 atgtttggcg cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt
     5161 ttaatgtcgg ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtccccagt
     5221 tatctcggct ccagcgcggc ttttgtcggc gtggtgattg ccgcgacggg ctttaacggt
     5281 cagggcatta acccgaatat cagcatcgcg ctgggcggga ttatcgcctg tgggctggtt
     5341 tataccgtca ttggcctggt ggtgatgaaa attggtacac gctggattga acgactaatg
     5401 ccgccggtgg tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgca
     5461 gtgaaaagtg tatcggcttc ggcctttgat agctggatgg cggtaatgac ggtgctgtgt
     5521 attgggctgg tggcggtgtt tacccgtggc atgatccagc ggttattgat tctcgtagga
     5581 ttaattgtcg cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc
     5641 gtcgatttta ctctcgtcag ctatgctgcc tggttcggtc tgccgcattt ttccactcct
     5701 ggttttaatg gccaggcgat gatgttgatt gcgcccgtgg cggtgattct ggtggcggaa
     5761 aacttaggtc atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg
     5821 gggcgagcat tcgtcggtga tggtctggca acgatgcttt ccggctctgt cggcggtagc
     5881 ggcgtcacca cctatgctga aaatatcggt gtgatggccg tgacaaaagt ctactcgacg
     5941 ctggtgtttg ttgcagctgc cgtcatcgct atgctgttgg gcttttcgcc gaaatttggc
     6001 gcgctgatcc ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg
     6061 ctgattgccg tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac
     6121 gctaatttga ttatggtcgc agtaaccttc gtgctgggcg cgggtgattt tgctctcacg
     6181 cttggcggtt ttacgttggg agggatcggt acagcaacct ttggcgcgat tttactcaat
     6241 gcgttattaa gcagaaggtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca
     6301 taaacagaaa accgccgggt taccggcggt gatgactttt tgacgagctt aagaaaaaaa
     6361 tgcattcgtc attgattagc ccggctagtt gtcgcttttg ccgtgactgc tcttgccacc
     6421 ttttttacct gcttctgatg ctctttcgcg gtcttctgca aaattgccgg aaccgcctcg
     6481 atggtttgcc attactgacc tccgtctggg tatagacatc atattgcata gaattaacgc
     6541 ggcgtatggc ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgcgga
     6601 cagacccgcg tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca
     6661 ataattatcc ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat
     6721 tttgcgacaa aattacgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat
     6781 ttgcactctg aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag
     6841 agataacacg aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac
     6901 ggacatattg aaacgatggc acgcgcagtc gctgagggtg caagcaaagt ggatggcgca
     6961 gaagttgtcg ttaagcgtgt accggaaacc atgccgccgc aattatttga aaaagcaggc
     7021 ggtaaaacgc aaactgcacc ggttgcaacc ccgcaagaac tggccgatta cgacgccatt
     7081 atttttggta cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac
     7141 cagacgggcg gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt
     7201 tccaccggta ctggcggcgg tcaggaacaa accatcacat ccacctggac gacccttgcg
     7261 catcacggca tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca
     7321 caggttcgcg gcggtacgcc gtacggcgca accaccatcg caggcggtga cggctcacgc
     7381 cagccaagcc aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg
     7441 gcagttaaac ttaacggcta atcttcaaca ggaggatacg catgccaact caagaagcga
     7501 aagctcatca cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg
     7561 ccattttcga agtcgccggg tacgacgaaa aaatggcaga aaagatttgg gaagaaggta
     7621 gtgatgaagt gttagtcaaa gcctttgcta agaccgataa agattcgctt ttttggggcg
     7681 aacagaccat cgaacgtaaa aacgtttaac cagcgactcc cccgcttctc gcgggggagt
     7741 tttctgttat ttcaccgcct cattcaacac gctatcaaac ttatccatcg ggcagaaacc
     7801 gttggcatct atcgggcaac cgcttaattc cagcgtcacg cgctgcgcag gtgcctgcag
     7861 ggttaacgca tcggcattac gtaactgttc cgcactctgg tacacatatt caattttcat
     7921 caaatcgcga ttggctttgc tgtcatgcca acgctggaaa acgattttgc cgccaatcgg
     7981 cgtgcgttcg ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga
     8041 ggctatgttg gagtcgtgcc caactaacac cgtgattttc ggagcactgg cgcgatcggt
     8101 gaccagggct ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg
     8161 tgaggtaaac agactgtcct ggtaaccgtt tttcagcttc gacaatacct tccactgctg
     8221 gtcagatttg atttctcccc aggccacctg atccatcgga aaaccttcgt agtattgcaa
     8281 agtaaacgca tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg
     8341 ttgatacttg gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt
     8401 acaggcaggg gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag
     8461 ctggagtttg ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga
     8521 atcatcggtg atcatcgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg
     8581 cacaggaata tcacacccag ggaatgcacc agtaataaag aactgggcgg tagcgacggt
     8641 acgttgcaga ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt
     8701 cactatcccc tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagcac
     8761 gccacctttg gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt
     8821 cgactgctcc agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat
     8881 catgagcact tgctgtagct gatagccttc cggtacggtt tgtgcctgag catttgaagc
     8941 gagtaaaact atccctgcca cagttgcggc gattagcgtt ttgttcattc ctggcacctc
     9001 ttttgttatc aatcattaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc
     9061 gcaaaatggc ttgttgaacg cctgtttttc tatatacatc ttcaaaaaca ggcaaggttt
     9121 gacgcaaact atgcctgatt agcccctgcc tcgcgtacgc cgctgataat tgcacctgtg
     9181 gctaaacaga atatctgcgg cgttaccaat acagagacaa tatttaccaa taaaaaagtg
     9241 tgagactaat aacaatggtt tttagcatat gcctttgcca ttaagttgca taacgattta
     9301 aatagtcaag ttgcgcattc atttccttat tttttaactt ataagcctga gaaaaaagtt
     9361 caggagggag gtaaacatca taatgattgc cagtgtttgt taaatatcca tactcaagat
     9421 cttcatctgt tgaaaaggat tcatttccca aggagattga tgccacatta ctattaacaa
     9481 taaataatga gcaaatgcta tctgaaagaa cagcaagaaa attcttcacc gctttaatcc
     9541 tatccgacgt gtcggtatct aaagcaagca aattcttgtt atcattaaat ataaactcgg
     9601 gtaaatgcag tatgcatttt ttgtcattca agcaactatc aatgaattgt tttttaaaaa
     9661 acagaaagct ccatgacata aaactaccca caccctgaat tttgttgtca gattgcagat
     9721 caacgccaaa taatgtcttt acatcttccc tgagattata gaatgccgta tcaacaagag
     9781 cctcgatcgt ttgtttatca acaattttaa taaactcttc gtatacttgc ttttgcgtct
     9841 tatggctgcc cagcgcccat gaatactcat gttcttttaa aaccagtatg gctcttaaca
     9901 gacaatatcc atcgccatct actggagcgt attttaatcc atatgtttta ttaatttcac
     9961 tctgaataaa agatatgcca gacaaatcaa taccagtatc taaatcagat attcccggca
    10021 tatttatctg ttctggctgg atactcatat tttcattatc taaacaatac tcagaaaaaa
    10081 catcatcaca cgcattaatg ttttcaatat attccataaa gccataccaa taataacctt
    10141 taaaatctgt gatagactct gaacctctgc ccggcaagga cactctaaca atatcaacat
    10201 cttgaacata ttgtatagta tatgtaatac ctcctgaaac ccctgaataa atttgtgtat
    10261 cattagtacg caatgttctc ataaataatg atagatcatt atcattctgc atttcctcaa
    10321 attctttttc tagtgattca cattccagac atggagtatc ctcttcattt actttacgtc
    10381 tgagttcatt gtacaaccgc ataaaacaca ccagtgtgtt ttcaaaaagc gtgattgaat
    10441 atgtttgatt tttttcgtac tcatttttta tatcgttcca ggcctgtttc aaataattat
    10501 ttgcagtgca actaattcca tgtatattac tacccatata tagcgtctat aaaatttaat
    10561 aaataatgac gccctagtta aacttaaagt gcctggttca actatcaaaa atcgctcacc
    10621 ctttttcacc tgtttaaaat atgttcagca acccatcttg atggcgacct cctctccgcg
    10681 atgatttcaa taacatattc tgtgttggca tatgaaattt tgaggattac cctacactta
    10741 taggagttac cttacagggg ttccttcaat ttgtgttgat ttacgcgaga taacgctatg
    10801 gaattaaagg attattacgc catcatgggc gtgaaaccga cggacgatct caagacaatc
    10861 aagaccgcct atcgtcgact ggcccgtaaa taccatcctg atgtcagcaa agaaccggat
    10921 gccgaagccc gcttcaaaga ggtcgctgaa gcctgggaag tgttaagtga tgaacaacgt
    10981 cgcgctgagt atgatcagat gtggcaacat cgcaacgatc cgcaatttaa ccgtcagttc
    11041 catcatggcg acggtcagag ttttaatgcc gaagattttg acgatatctt ctcgtcaatt
    11101 ttcggtcagc atgcccgcca gagccgtcaa cgccccgccg cacgtggcca cgatattgaa
    11161 atcgaagtgg cggtattcct cgaagaaacg cttactgagc ataagcgtac catcagctat
    11221 aacctgccgg tttataacgc ctttggcatg atcgaacagg aaatcccgaa aacgctgaat
    11281 gtgaagatcc cggcaggcgt cggcaatggt caacgcatcc gcctgaaagg ccaggggacg
    11341 ccgggcgaaa acggcggtcc aaatggcgat ttgtggctgg tgattcatat tgcgccacat
    11401 ccgctgttcg atattgtcgg ccaggatctg gaaattgtgg tgccggttag cccgtgggaa
    11461 gcggcgctgg gtactaaagt caccgttcca acactgaaag aaagcatttt gctgactatc
    11521 ccgccaggca gccaggccgg gcaacgattg cgcgttaaag gcaaaggtct ggtgagcaaa
    11581 aaacagaccg gcgatctgta tgcggtactg aaaatcgtga tgccgccgaa accggatgaa
    11641 aacactgccg cgctgtggca gcaactggca gacgcccagt cgtcttttga tccacgtaaa
    11701 gattggggga aagcataatg gctaatgtta cggtgacttt tactattacc gaattttgcc
    11761 tgcataccgg catctctgaa gaggagttga atgaaattgt cggtttgggg gtggttgaac
    11821 cgcgtgagat ccaggaaaca acctgggtat ttgacgacca tgccgccatt gtggtgcaac
    11881 gcgcggtacg cctgcgtcat gaactggctc tggactggcc ggggatcgcg gtggcgctga
    11941 cgttaatgga tgatattgcg cacctgaagc aggaaaaccg cctgctacgc cagcggcttt
    12001 cccggtttgt agctcatccg tgatgtgtgt ttgccggatg tgatgacgca tccggcaact
    12061 gcgccataat gcctgatgcg acgctaaacg cgtcttttca ggcgcataaa cgacgttatc
    12121 tgttttggtg gtcgcactcc actaacacca gcaataactg gcttagtgcc gcgtaaaaac
    12181 caaagctgtc atactgtcga caacgcgcag caaactctgg taaccattgc cgcagcgccg
    12241 tcagtgtttt ttgccgcaaa ctgtcgattc ttcgcgcagg aacggtcccc tctcccagcg
    12301 aaaaatgcag ataactcaac aactcgagat agatcgccag atgatctgcc ggttcgttga
    12361 aattgccact ggtttccatc cctgcctcaa ctaacaagcg tttaatctct tgctcgtcct
    12421 gtttgtaggc cgatgcatac ggcagcgccg cttgtttgtc ggtcatcaga aacaggccgc
    12481 aaaagtcagc ggccagttcc agacgggcat cgtcacgtac tgtcagcgt
//
LOCUS       DAJTRN010000105        12391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRN010000105 DAJTRN010000000
VERSION     DAJTRN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1635
                     /gene="maeA"
                     /locus_tag="OO328_003235"
     CDS             <1..1635
                     /gene="maeA"
                     /locus_tag="OO328_003235"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5931692.1"
                     /translation="EFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQ
                     GFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSR
                     GVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTA
                     CGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWP
                     DVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKK
                     IVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKL
                     VQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIV
                     MPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIG
                     LGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKM
                     AQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1769..2779
                     /gene="adhP"
                     /locus_tag="OO328_003236"
     CDS             1769..2779
                     /gene="adhP"
                     /locus_tag="OO328_003236"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5931693.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            2925..3209
                     /locus_tag="OO328_003237"
     CDS             2925..3209
                     /locus_tag="OO328_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5931694.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3437..4090)
                     /gene="fdnI"
                     /locus_tag="OO328_003238"
     CDS             complement(3437..4090)
                     /gene="fdnI"
                     /locus_tag="OO328_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5931695.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4083..4967)
                     /gene="fdnH"
                     /locus_tag="OO328_003239"
     CDS             complement(4083..4967)
                     /gene="fdnH"
                     /locus_tag="OO328_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5931696.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4980..8027)
                     /gene="fdnG"
                     /locus_tag="OO328_003240"
     CDS             complement(4980..8027)
                     /gene="fdnG"
                     /locus_tag="OO328_003240"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7440..7442),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5931697.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8259..9140
                     /gene="yddG"
                     /locus_tag="OO328_003241"
     CDS             8259..9140
                     /gene="yddG"
                     /locus_tag="OO328_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5931698.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9395..10495
                     /gene="ompC"
                     /locus_tag="OO328_003242"
     CDS             9395..10495
                     /gene="ompC"
                     /locus_tag="OO328_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5931699.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10659..11144
                     /locus_tag="OO328_003243"
     CDS             10659..11144
                     /locus_tag="OO328_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5931700.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11254..12243
                     /locus_tag="OO328_003244"
     CDS             11254..12243
                     /locus_tag="OO328_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5931701.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3244 a         3072 c         2885 g         3190 t
ORIGIN      
        1 gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac
       61 ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg
      121 atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag
      181 gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct
      241 gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt
      301 tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac
      361 gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt
      421 ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc
      481 tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac
      541 caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgat
      601 gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg
      661 ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa ccgctatcgc
      721 aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac agtcggcaca
      781 ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt
      841 ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa
      901 ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt tggcctgctg
      961 acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac
     1021 ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt gcgcaatgta
     1081 aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc
     1141 cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtct
     1201 cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc gctggtcgcc
     1261 actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat cgcccagtgt
     1321 aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg cgcgtcacgt
     1381 atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc
     1441 ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca
     1501 attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa aacgtctgct
     1561 gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg cgactaccgc
     1621 cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt ccctctccct
     1681 ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat
     1741 gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga aggatcatca
     1801 tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat
     1861 ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa
     1921 aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt
     1981 cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag gatgcggtca
     2041 ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata
     2101 cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg cggtaaaagt
     2161 gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg tcaccaccta
     2221 caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct acggtcttgg
     2281 cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc
     2341 cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag atttagcgat
     2401 taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc
     2461 tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc
     2521 aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg
     2581 tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac
     2641 cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt
     2701 agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt
     2761 gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt cgcagcatcg
     2821 cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg aacaataatg
     2881 atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa atggccaatc
     2941 atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg
     3001 agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag
     3061 cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa
     3121 tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg gtggttgtgt
     3181 cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg ccatttgaaa
     3241 ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca cgttcacttc
     3301 aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc atccgggatg
     3361 aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc attttcagct
     3421 taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga
     3481 tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
     3541 tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt
     3601 ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg
     3661 ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa
     3721 tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta ccaacatccg
     3781 ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt
     3841 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa
     3901 tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
     3961 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
     4021 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
     4081 acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
     4141 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
     4201 gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac
     4261 agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
     4321 gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc
     4381 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
     4441 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
     4501 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
     4561 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
     4621 ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta
     4681 cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat caccgtccag
     4741 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
     4801 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
     4861 acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
     4921 gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg ttcgccccct
     4981 tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca
     5041 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
     5101 cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga
     5161 cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac agtgacacga
     5221 tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac aaactgttcc
     5281 ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga
     5341 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag cgcgtcttgt
     5401 tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc
     5461 ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
     5521 aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt acccggtgcg
     5581 gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc
     5641 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg
     5701 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
     5761 gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt gtagatccag
     5821 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
     5881 ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata gccgttgttc
     5941 tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
     6001 atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag atgatggtag
     6061 atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag
     6121 tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc aaagcaggtc
     6181 gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg
     6241 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
     6301 agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac cgggttaaag
     6361 ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata cttgatgacg
     6421 tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg
     6481 gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag
     6541 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
     6601 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag cagacctaag
     6661 tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
     6721 cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
     6781 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
     6841 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca gatgttttca
     6901 acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac acagcgcgga
     6961 tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
     7021 gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc ttcgaaagca
     7081 aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt
     7141 ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc cgtaccggaa
     7201 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg atcgacaacg
     7261 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
     7321 gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag
     7381 tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
     7441 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgggtc
     7501 agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg
     7561 tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc
     7621 agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
     7681 ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc cagcaaaccg
     7741 gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
     7801 taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa tagcccgcaa
     7861 cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt
     7921 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc
     7981 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
     8041 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
     8101 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
     8161 tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca
     8221 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
     8281 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
     8341 gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
     8401 taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg
     8461 ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg tatgcggcga
     8521 cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc
     8581 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
     8641 tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa
     8701 tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta
     8761 tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
     8821 tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag
     8881 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
     8941 ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag
     9001 gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
     9061 tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc
     9121 tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa
     9181 attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc
     9241 gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc
     9301 attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa
     9361 atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt
     9421 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
     9481 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
     9541 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
     9601 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
     9661 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
     9721 ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat
     9781 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
     9841 caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
     9901 tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt atggtgctga
     9961 cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
    10021 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc
    10081 taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga
    10141 tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg
    10201 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
    10261 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta
    10321 cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
    10381 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
    10441 cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc
    10501 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
    10561 aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat
    10621 atcctccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
    10681 cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa
    10741 ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg
    10801 gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
    10861 ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata
    10921 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg
    10981 ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac
    11041 tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa aatcagcatt
    11101 cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat
    11161 aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa
    11221 gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat
    11281 catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa
    11341 aacactacaa aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac
    11401 attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat
    11461 gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat
    11521 gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac
    11581 ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat
    11641 agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc
    11701 agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata
    11761 gataggcttt catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa
    11821 ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt
    11881 gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa actaacaagt
    11941 ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct
    12001 gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac
    12061 cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga
    12121 ctggacaata ataaaataac ccacattgat actaataata catccgatat tggaacttta
    12181 ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat
    12241 tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact
    12301 ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat
    12361 aacgaattgt ttcactatac tattaaaaaa g
//
LOCUS       DAJTRN010000106        12357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRN010000106 DAJTRN010000000
VERSION     DAJTRN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            79..155
                     /locus_tag="OO328_003245"
     tRNA            79..155
                     /locus_tag="OO328_003245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Arg,seq:tct)
     gene            complement(201..962)
                     /gene="envY"
                     /locus_tag="OO328_003246"
     CDS             complement(201..962)
                     /gene="envY"
                     /locus_tag="OO328_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5931702.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1145..2035)
                     /locus_tag="OO328_003247"
     CDS             complement(1145..2035)
                     /locus_tag="OO328_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5931703.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2036..5004)
                     /gene="nfrA"
                     /locus_tag="OO328_003248"
                     /pseudo
     CDS             complement(2036..5004)
                     /gene="nfrA"
                     /locus_tag="OO328_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(4991..7228)
                     /gene="nrfB"
                     /locus_tag="OO328_003249"
     CDS             complement(4991..7228)
                     /gene="nrfB"
                     /locus_tag="OO328_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5931704.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7378..8820)
                     /gene="cusS"
                     /locus_tag="OO328_003250"
     CDS             complement(7378..8820)
                     /gene="cusS"
                     /locus_tag="OO328_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5931705.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(8810..9493)
                     /gene="cusR"
                     /locus_tag="OO328_003251"
     CDS             complement(8810..9493)
                     /gene="cusR"
                     /locus_tag="OO328_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5931706.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9650..11032
                     /gene="cusC"
                     /locus_tag="OO328_003252"
     CDS             9650..11032
                     /gene="cusC"
                     /locus_tag="OO328_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5931707.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            11056..11388
                     /gene="cusF"
                     /locus_tag="OO328_003253"
     CDS             11056..11388
                     /gene="cusF"
                     /locus_tag="OO328_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5931708.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            11404..12123
                     /locus_tag="OO328_003254"
                     /pseudo
     CDS             11404..12123
                     /locus_tag="OO328_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2975 a         3162 c         3219 g         3001 t
ORIGIN      
        1 gcaaccagat caaaagccat tgactcagca agggttgacc gtataattca cgcgattaca
       61 ccgcattgcg gtatcaacgc gcccttagct cagttggata gagcaacgac cttctaagtc
      121 gtgggccgca ggttcgaatc ctgcagggcg cgccatttct tcttcattta tgcccgtctt
      181 atccgtttcc gctttgccct tcaccacatc actttttgtc gctgtttggc gagataattc
      241 aacggtgtca ggctgtaaaa cgccttaaaa acagagataa agtacgacgt gctgctatag
      301 ccacataatt gcgccacctg agtgatattt ttgttatcca tcaataacat ctgtacggcg
      361 taacgcatac gacactctgt gacaatctgg ctatagctgg tattttcgtt ttttaatttc
      421 tttttgagca ggctggggct taaacatagc gaactggcga caattcgcag attccagtaa
      481 tgctgaatat cgctttgaat aatgcggcag acgctgtcgc ggacgctgct gcgtaagata
      541 tacatcagta gggcaataaa ccgcgattgc tcaagaaagt tagacaatac ggtaaaaagt
      601 aatgcgcgcg tcaactccgc ttcgcagggt ttgctggatt gctgcttgct gtggttggtg
      661 gctagccgga acacttccgg tgtacggcta tgacaagcga taaccggggt tgctagccgc
      721 gaccagggct ttacacaggt taatttctta tttaaaaaca acagataatc gttgatgatg
      781 ttacggttga tgtgggtgat taatacgtga tttaacgttg agagttcaat gacgttattg
      841 ttgcacgcga aggcggccag gtagtttttc ggaagggtaa acgtagcatg gttattgacg
      901 cttacctcta cctctttttc ggtcaggatc accacgcaag gttcactgct gctcaattgc
      961 atttcgcact cctcagatat cagaaactcc gctcaaagga tctatgcttc ctgcatgagt
     1021 gatcggcccg ttcgccgata acgatcttct ttctttagca tgctttttag caattaatct
     1081 tgatggaatt ctgacgagag cgaaagaggt aagccaggtc gtacccgact tacctggagg
     1141 ggatttaata ctcgagaatg ccgtgcgcga cgggcaaata gcgcagagag aaatagagca
     1201 tgtctttacc gcaagaggaa cgtttcgcta acagcgaggc aatttctgcg tccggtttcg
     1261 gtttcgctgt aaaggttttg cctttgccgg cgacaaacag ttcataaaca atgccgcttg
     1321 ctggggcgga gctttggcaa tcggcgctgg cctgtagata acgttcacgc tgttcagcgg
     1381 taagtttatc cacgccgctg ccatcctgta cccagacatt aactccggtt gctttaactt
     1441 gttccagcag ttggcgatag ccatcgggcg acatgtttcc ggcgaaaaaa ctactgatat
     1501 aaaccggttt tgctgaaaca tcgctaatca gccgctgcgc gttgtttaac catgttagca
     1561 aaggttgacg ggcggcttcg ctgcgccagt tcaggtcgtc aatttccgcg ctgatgtacc
     1621 agccatccgg cgttatgcca ggcgcggcgc tccataatct ggcttgctgg agatcggcag
     1681 ccagcaggcg attaagatag ctttccagcg ctgcagacga ctgtttctgg tgcataaaaa
     1741 attccggatc ggcgttcagc ccgacaatca gcttcaggcc agtctgttgc gcagctgcgg
     1801 cacgcttaaa caataacgtg cgctgttctg gctgggtaaa tgcatcgccg taacgggtcc
     1861 attgcaaaac cagggtatcg aagccttgaa aacgtaactg actcatcagc ccctgccact
     1921 gggtatcggt aacctgacta tctcggtttt gtggttgcca gataatacct ttcatcgcaa
     1981 aggaaaaagg gctgaccaaa agcagtgtca gcaatacgaa aatgaactta cgcatttacc
     2041 agtgcactcc aatggtgaga aacgcgttgt tgcgctctcc gttacgttga ttaatcgcct
     2101 taaaggtatg ttgatactcg acgccgagac tgactttgtg cggccaggcg tcgtagtgcg
     2161 tctcgccggt ccaaatattc cagcggaccc cgacaccgcc aagctgcgcg ccctgagtgc
     2221 ctttatcacg atagccgttg tcctgaacgt gagcgtaagg ctcaatagtc tgtccgttag
     2281 ctaccttctg atgccagctg acgcgataat ctgccgtcca cgcctgaata tcctggcgga
     2341 tatattgcgc cgcatcgagg tacaggtttt gggcaaacca gcctgaaccg ttcgggtgcc
     2401 attcgtcgct gtatttgccg ccattaaaga atgaggcgct ggcgcgcagc atggtatcgg
     2461 atgcgccatt ttgcccgttc agcggcaact gctgttcgac ggcgaggaaa aagatctgat
     2521 cgcgcagcgg cttccagcgc agaccggtgc cggacatcgg atttttcacc ggcatcatca
     2581 ccccgttttc tccggtatcg gcaaagacgc ggctataaac tgagagcagg tcgccttcca
     2641 gcagcatatt gcgtccgatg cggtactcgg cttccagttg tccgtagcta cgatagcttt
     2701 tcccaggcgc tgcaccgccg acattattgt tagcggtact cattgcgccg gaacgcaagc
     2761 cgatggaaga atcgaaactg aacgtccagc ggcgaccgac ctcctcatgc aaacggcgga
     2821 aattgaagcg ttgctgattt tgctctgggg tcagtggggt tatgcctgat tatcaatgtc
     2881 atcaatcacg agccgggcgt agtgctgcgt cgcaggcatg tcatccagac gctggttcac
     2941 gtaggccagt tgtcggatca gtgccggatc gtccggaagc cctttatgcg cctgttcgag
     3001 catttcccgc gactgtgcga tatcaccgct atcccacaag gcgtaaccaa gcgctgcctg
     3061 ggtgttgcta ttatttggtt ccagttccag cgcggcgcgc aaatcactca ccgcggccgg
     3121 aacattatga cgttggcgat aaattgtcgc ccgcgcaacg taagagttgg cagaaggggc
     3181 aatattgatt gagcgcgtga gatcgttcag tgcgagttct ggctgagctg ggatataacg
     3241 tcgcgcatgc agccaccagt attgagcatt gtttcccagc tcacgttgtt ctgcctgttg
     3301 aagccagcga tcgcgagctg cgccatttcc cgctgcctga gcggtgttgg cggcggcgag
     3361 cagatcatca ttgctcatgt cgtgaagact gattttctgc caggccgcca gtgcggtggc
     3421 gtagtcctca acctgatacg cctgataggc taccgcacga tgttgccagg cgctcggttg
     3481 tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg ggtagcgtgt cccgataaca
     3541 ctttgccaga cggttccagg cggcagcatc gtaggaaggc gacatatcgc ccagcaagcg
     3601 aactattgcc gggcaattat ctgcaatacc cggcaactga ctttgccact gacgttgctc
     3661 cgccagcggt aagggtttcg ataaaatcgc caccttcgcc ggcgttgcta ggtaaggatg
     3721 actttccagc agagacgcca gtcgcgccat taaagtctgg ctgacacgcg catcgccctg
     3781 gaaaggatag cgttgcagca ataaatcggc agcttcgcgt gactgctcgt tctgcatcag
     3841 ttgccaggtc agttgatcca ggcggggaag atttgtcggt tcttgctgat acagcaatcg
     3901 tgccagacgc agagattcag ccttgttacg ggtcgccacg ctgacagcat aacgctcctc
     3961 aagcatttca ttggcgggga gggtggcgag cagtttttgc gctgcgtcgt actgaccttc
     4021 ttttaacagc accggtagcg tcgcgccaac aacatactgg cggttatcgg caaactgcac
     4081 cgtataattc gccaatgcct gaacggggtt ggcgctgtat ttagataaca gatagagcca
     4141 acttttctct tgtgcgcccg tggtaaacag cggcttattt tcaatgagat aatgctggag
     4201 gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa gtaatatatg actgaggatc
     4261 ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg tccagctgcc cggcaagaag
     4321 cacgtcaaac cactgacggc gttctgccgc gcttaatgtg ttctgctggc gtgcttcatt
     4381 gtatagcgta tctgcctggg atcattgttt caggtagatt gcccgttgca gcagatcggt
     4441 tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca tcgttcagtt gcgctctggc
     4501 gacaggtaat tgtgccagcc gcagggcatt ctgcccgact tcactgcgac aacgcagggt
     4561 cggcgcagcg tcgcccgctt tttgctgggc aagcagttct tcaacagtcg taacgctttt
     4621 cacttcaact ggaatagccg ccagactgcg ctcaagtcgg gcatctcctg ggtgacgttt
     4681 cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga ccaaaatgac gataggcttc
     4741 cgcaaggtat aaagtcagcg gaatattatc cggcacctgt tggtgtatat attcaaattc
     4801 gcggatggcg gttgcttcgt cgttattttt ctgtgccttc agcgccttat cgagacgggg
     4861 ataaataaca aaatggcgat aatcgctcag ccccagctct tctgcgctgg tgccgatatt
     4921 gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt aaaccagacc atccgatgac
     4981 gcgattaagg ttattctcct tcattttcgg actccagttg cgcaacctgt tctgtgttta
     5041 aacctgcttt gagtaatagt gattgcatcg aaacttgtaa ttcgcgttga attgtcagga
     5101 cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac caaaaacttg ccgagcggca
     5161 gagaactgcg ttcatggcgc aataacaaca cgttaattgc tgaacgatta atatgaccga
     5221 gcgtggtcag tatttcggcg aacaggaact gatgcggcac atattgccgc cagatttcac
     5281 cggtctgctg ttccgtgagc cactgatgct gaaccgcatt gtacaacatt gcccgcggat
     5341 cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt gacaatttgt ccccgcagaa
     5401 caatgacgta acgcactttg cgtccgactt tacgcgtcag ggccgccagc gaaaccgggt
     5461 caataccatc ttcactgccg acaattaact catcattttc cagacgcagc ggcagtactg
     5521 cataatgcag cgctacggag gctggcattt cggcaatcag cgaggacggg atctgccagg
     5581 catcgatgga ttcccacgcc acgccgtttt gctctgccag cgcctgtgcc agctgctcgg
     5641 cgctaatcag cccctgcatc agcattgaac cgcccaggcg tagaccttcg acgcgattac
     5701 gcagtgctgt atcgagttgt tcttcagtga tgacctgatt ttccagcaga atttgaccta
     5761 acgggcgcaa cgagcgggta tcgccagtga cgctggggaa gtcatgcgtt gttttatccc
     5821 acgcgacgcg acgcggatcg ccgtgctgaa gaacctgttt cagcacgcgc cagttagcca
     5881 taaagttaat caggttgccc cagaaaagac gcaggacgga aagcaggccc tgcgtgaggc
     5941 cgtagtagcc agtgacgaaa atcactcgct gcacgatgcg gttaaccatc aagccaaagt
     6001 ttagccacag cagggtcatt aaccatgcgc tgccgctaaa aatagaaagg aaatgccagg
     6061 catcgggcca caaactttca tacgccagca acagcaaaag ctggatcatc accagcatcg
     6121 cgaggaagct gacaaagtta ctgattgccc ctttgcggtc gcgccagaga aagtagttca
     6181 gcgtcaggct ggaggtccat ttatgggttt taaagccctg gaaaacaatg ccgatgatcc
     6241 agcgagattt ttgtcgaacc gcagtcgaaa aggtatcggg gaaatattcg cgcacgcaga
     6301 tcatgtttga cgtccgtgcg tgctgtaaaa atttacgctg ctcgcgttct ttggcttcgt
     6361 ccaccaccgg aaaacggaca aaaatttccg tcataccttt ttctttcagg cggaagccaa
     6421 tgtcgtaatc ttcagtaaga ctctgcacgt cgaaagcaat accgttaccg tcagctaaca
     6481 gtgcggtcac ggcgcggcgg ctgaaacagg tgccgacgcc tgcgctgggc acttgtccgg
     6541 cgagggcttc acgcaccgga acatctttgc catgcagttc tgaaaactca tcaatgtaag
     6601 tcatgctggt gaagtgcgtc cattcgcgtt caaacggata cactgggatc tgaatcagat
     6661 ctttacgctc gaccagatag ttgaacagac gcaattccat cggtgaaatc acatcttcgg
     6721 cgtcatgcag aataaaacca gcaaaagcga aattggcgct acgttcaaac tgggtgatgg
     6781 cgtccagcac gttgttcagg cagtcggctt tgctggtggg accaggacgc gcgcagacta
     6841 ccttatgcac gttcggaaag cgagcgcaca cttcgtcaac atcacgttga gtatcggggt
     6901 cgttggggta ggtgccaaca aagatatgat agttttcgta gtcgagcgtg gtcgccgcca
     6961 gctcggccat attgccgatg acgcccgttt cattccacgc cggaaccata atcgctaacg
     7021 gtttttcatc tggtttatac agttcgcggt aactcattcg cgggtagcgg cgataaacac
     7081 tcaacttgcg tttaatgcgg cgtacccagt agacgacatc aataaaaaaa tcgtccagcc
     7141 cgctgatgaa catgatgacc gctaacgtta tcgcgattac ttttaagccg tagagccagg
     7201 tagcaaaaac atcaagaagc cagtccacac aaaaacctta cattaacgct ggttatgttt
     7261 agggtggcgt atattaaggt tttttatgaa ttgtgacagc tttttaccat taatatgtat
     7321 gactattgcg gcacgttatt tttacactgg ttataaaagt tgccgtttgc tgaataatta
     7381 agcgggtaat gtgataacaa accttgtccc ccgcgcatct gacgttaccg caaccgtgcc
     7441 tttatgcgcg acaacaatcg atttcactat cgccagccca ataccgctac cttcaccttt
     7501 tcgctggcgg gaggggtcaa cgcgatagaa acggtcaaac aatcgcggta agtgctcggg
     7561 cgcaatgggc gtaccggggt tttcgacgga aacttgcacc tggtgatcga ccgtctgaca
     7621 gcgtactaca attgtctctc tggttggcgt ataacgaagg gcattagaaa gcaggttgct
     7681 tagcgcccga cgcagcatca acggatcgcc cgcaacctga cgtttgtcgc caacaaaccg
     7741 caactccacg ccgcgatctt ccgctagcgc ctcgaaaaaa tcgaacactt tgccgacttc
     7801 atccgccagg ttgagcattt tcttttcggg gattagctgg ttgttatcgg cctgagcgag
     7861 aaacagcata tcgctgacca ttttcgccat tcgcgtcagc tcttcgagat tagagtagag
     7921 cacatcttcc agctccttct ggctgcgcga ctggctgagg gcgatttccg tttgcgtaat
     7981 gagattagta attggtgtgc gaatttcgtg ggcgatatcc gctgagaaat tggactggcg
     8041 agtaaagaca tcctcaatac gctcgatcat atggttgaac gacagtacca gctgttccag
     8101 ctcaatcggc acggtctgcg ggtcaaggcg aacgtcgaga tctttcgagg tgatattctg
     8161 gatttgacgg ctgacgctgc ggatcggcgc gtgaccttta tgtaccgcca acagcacgat
     8221 aaagacgatc aggatgctga ttaccgatgc ggtcataata agtttattca tcaaatcatt
     8281 tatgtaatga agatgaaaat cgatcgacag cgcgatgtag agcgtataaa tcggtttgcc
     8341 gtccaccaac gggccaaccg gcaagttaat catccgccag ttgctgtgtt ccatatgccc
     8401 gtgaccgtgg cctggcatca taatcgtcgg gccggaaagg agatacacct cgccaccccg
     8461 cgcgtcttta tcgggtatgg catcacgtgc aaactcgcgg atatcaggcg caccggggga
     8521 gtgatacacc gttttgccgt ggctatctgc cagggaaatc aacacgttgg aataaccact
     8581 gacgatatct tccagcgtca ttaagcgtcg ggcttgcgtt tcgtcagggt gatttagtac
     8641 ccgttcaagg gtggcgctaa tctcttttaa atcattaatg tcctgctcgg caaaatgcac
     8701 ttttactgag tggattatga tccatgcaaa ggcgaaaaac gccgcgatgg tggcctggct
     8761 gataaaaaag gtcaggcggg ttgccagcga aaacgggcgc tgaaatggct tactgaccat
     8821 ccggcacctc aagcatgtaa cccacgccgc gcacggtttg aatcagcttc ggctcaaagt
     8881 cgttgtcgat tttgccgcgt agcagcttca ccgccacatc aatggcgtta gtgtcgctgt
     8941 cgaaattcat gtcccatacc tgcgaggcga taagcgagcg gggcagcact tcgccctgat
     9001 ggcggagaaa aaactccagc agagtaaatt ctttactggt cagagtgatg cgcgtgccgc
     9061 tgcgggtgac tttgcggctg acgagatcga ccatcagatc ggcaacctga aactgacttt
     9121 cgataatcac cgccgccccg cgccgcagga gagtgcgcac ccgcgccagc agttcagcaa
     9181 aagcgaacgg tttcaccagg tagtcatctg cccccaactc cagccccttg acgcgatgtt
     9241 caatggtgcc aagcgcggta agcaacagaa tcggcatccc tttattggcg gagcgcaaca
     9301 tgcgcacgat atcccagccg ttcacgtctg gcagcataat atcgaggatt atcaggtcat
     9361 aatcaccggt catcgccaga tggtagccat tcagcccgtt gtcggccaaa tcgaccacaa
     9421 aaccggcttc ggttaaccct ttggtcaagt attctccggt tttcttttca tcttcgacaa
     9481 tcaacagttt catatttcct ccgcatgttg cccgggcaat tctagagtag cgggatcaga
     9541 tggcaatcgc ttattggcaa aatgacaatt ttgtcatttt cctgtcaccg gaaaatcaga
     9601 gcctggcgag taaagttggc gacataaaat caccagaaat tatgagccta tgtctccttg
     9661 taaacttctg ccattttgtg tggcccttgc gctaactggt tgttcactgg caccggatta
     9721 tcagcgtccg gtaatgcccg taccgcagca gttctcactc agccagaacg gcctggttaa
     9781 cgcagcggat aactaccaga acgtgggctg gcgcaccttt tttgttgata atcaggtgaa
     9841 gacgctgatt agcgaggcgc tggtgaataa ccgggatttg cgcatggcga cgctgaaagt
     9901 gcaggaggca cgggcgcaat atcgtctgac cgatgccgac cgctacccac agctcaatgg
     9961 cgagggcagc ggcagctgga gcggcaatct taaaggcgat tcagccacga cgcgggagtt
    10021 ctcgactggc cttaacgcca gctttgatct cgattttttc ggtcgcttaa agaacatgag
    10081 cgaagccgag cgccaaaatt atttagccac tgaggaagcc cagcgcgcgg tgcatatttt
    10141 gctggtttct aatgtcgcgc aaagctattt caatcagcag ctggcgtatg cgcaattgca
    10201 aatagccgaa gaaacgctgc gtaattatca gcagtcatat gcgtttgtcg aaaaacaact
    10261 gttgaccggt agcagcaatg ttctggcgct ggaacaggct cgcggggtga tagaaagtac
    10321 ccgcagtgac atcgctaaac gtcaggggga actggcgcag gcgaataatg cattgcaact
    10381 gttattggga agctacggca agctaccgca agcgcagaca gtaaacagcg gcagcctgca
    10441 aagcgttaaa ttaccggcgg gcttgtcgtc gcaaatctta ttgcagcgcc cggatattat
    10501 ggaagctgaa cacgcgttaa tggcggctaa tgctaatatt ggtgctgcgc gtgcggcatt
    10561 tttcccgtct atcagcctga ccagcggaat atcaaccgcc agcagcgatc tgtcgtcatt
    10621 atttaacgct agcagcggga tgtggaattt tattcctaaa attgagatcc ccatttttaa
    10681 tgccggacgc aaccaggcca atctggatat cgccgaaatt cgccagcagc agtcggtggt
    10741 gaattatgaa cagaaaatcc agaacgcctt taaagaggtg gcagatgcgc tggcattacg
    10801 tcaaagcctg gacgatcaaa tcagcgccca gcagcgttat ctggcgtcgc tgcaaattac
    10861 cctgcaacgg gcgcgggcat tatatcagca cggcgcggta agttatctgg aagtgctgga
    10921 tgccgagcgt tctttatttg caacccgaca aactttactt gacctgaatt atgcccgtca
    10981 ggttaacgaa atttatttgt ataccgcctt agggggcggt tggcagcaat aatttttaac
    11041 tccaggagag aataaatgaa aaaagcactg caagtcgcaa tgttcagtct gtttaccgtt
    11101 attggcttta atgcccaggc taacgaacat catcatgaaa ccatgagcga agcacaacca
    11161 caggtcatta gtgccaccgg tgtggtaaaa ggtgttgatc tggaaagcaa aaaaatcacc
    11221 atccatcacg atccgattgc tgccgtcaac tggccggaga tgaccatgcg ctttaccatc
    11281 accccgcaga cgaaaatgag tgaaattaaa accggcgaca aagtggcgtt taattttgtc
    11341 cagcagggca acctttcttt attacaggat attaaagtca gccagtaacc caggtttaat
    11401 gagatgaaaa aaatcgcgct tattatcggc agcatgatcg cgggcggtat tatttcagcg
    11461 gcaggtttta cctggtttga ccaggcggaa ccgcacgcag aaaaaatgcc gaccgcagaa
    11521 cgtaaaatct tattctggta cgacccgatg tatcccaata cgcggttcga taaaccaggt
    11581 aaatcgccgt ttatggatat ggatctggtg ccgaaatatg ccgatgaaga gagttctgcg
    11641 tctggtgtgc gcattgaccc gactcagacg cagaatctgg gggtgaaaac ggctaccgtc
    11701 acgcgcgggc cgctgacttt tgcccagagt ttcccgacga atgtcagtta caacgagtat
    11761 cagtatgcca ttgtgcaggc tcgcgctgcc gggtttatcg acaaggtgta tccgcttacc
    11821 gtgggcgata aagtacaaaa gggcacaccg cttctcgacc tgaccattcc cgactgggtg
    11881 gaagcgcaga gtgagtattt actgttgcgc gaaaccggcg gtacggcgac ccagactgaa
    11941 ggcattcttg agcggctgcg actggcggga atgccggagg cggatattcg ccacctgatc
    12001 gccacgcaaa aaatccagac tcgctttacg ctcaaagcgc ccattgatgg cgtgatcacc
    12061 gcgtttgatc tgcgcgcggg aatgaatatc gccaaagata acgtggtcgc gaaagaggaa
    12121 ggtaggttgc ttatctgtgt ctgtcattgt ctagcattgt ccactgtagt taattaactg
    12181 tctgttataa ctcgaaaaac aacctttttt tcacctgagt atgtctacga acgtcaaata
    12241 tagttgttta ctgtgcgctg ttgtccatgt gatattgtgt accagatgtg taccaaacca
    12301 ttatttctga gcgtaccaaa tattatttat ggctataagc gacacaaaac tgcgtac
//
LOCUS       DAJTRN010000107        12212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRN010000107 DAJTRN010000000
VERSION     DAJTRN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..484)
                     /locus_tag="OO328_003255"
     CDS             complement(<1..484)
                     /locus_tag="OO328_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5931709.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     L"
     gene            complement(515..1207)
                     /gene="fimC"
                     /locus_tag="OO328_003256"
     CDS             complement(515..1207)
                     /gene="fimC"
                     /locus_tag="OO328_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5931710.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1427..1969)
                     /gene="fimA"
                     /locus_tag="OO328_003257"
     CDS             complement(1427..1969)
                     /gene="fimA"
                     /locus_tag="OO328_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5931711.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2450..3316
                     /gene="folD"
                     /locus_tag="OO328_003258"
     CDS             2450..3316
                     /gene="folD"
                     /locus_tag="OO328_003258"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5931712.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3318..3530
                     /gene="ybcJ"
                     /locus_tag="OO328_003259"
     CDS             3318..3530
                     /gene="ybcJ"
                     /locus_tag="OO328_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5931713.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3638..4147
                     /locus_tag="OO328_003260"
     CDS             3638..4147
                     /locus_tag="OO328_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5931714.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4183..5568)
                     /gene="cysS"
                     /locus_tag="OO328_003261"
     CDS             complement(4183..5568)
                     /gene="cysS"
                     /locus_tag="OO328_003261"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5931715.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5742..6236
                     /gene="ppiB"
                     /locus_tag="OO328_003262"
     CDS             5742..6236
                     /gene="ppiB"
                     /locus_tag="OO328_003262"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5931716.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6239..6961
                     /gene="lpxH"
                     /locus_tag="OO328_003263"
     CDS             6239..6961
                     /gene="lpxH"
                     /locus_tag="OO328_003263"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5931717.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7079..7588
                     /gene="purE"
                     /locus_tag="OO328_003264"
     CDS             7079..7588
                     /gene="purE"
                     /locus_tag="OO328_003264"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5931718.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7585..8652
                     /gene="purK"
                     /locus_tag="OO328_003265"
     CDS             7585..8652
                     /gene="purK"
                     /locus_tag="OO328_003265"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5931719.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8789..9682)
                     /locus_tag="OO328_003266"
     CDS             complement(8789..9682)
                     /locus_tag="OO328_003266"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5931720.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9679..10326)
                     /locus_tag="OO328_003267"
                     /pseudo
     CDS             complement(9679..10326)
                     /locus_tag="OO328_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10329..11561
                     /gene="allC"
                     /locus_tag="OO328_003268"
     CDS             10329..11561
                     /gene="allC"
                     /locus_tag="OO328_003268"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5931721.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11572..>12212
                     /gene="allE"
                     /locus_tag="OO328_003269"
     CDS             11572..>12212
                     /gene="allE"
                     /locus_tag="OO328_003269"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5931722.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2965 a         3155 c         3115 g         2977 t
ORIGIN      
        1 tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa tcaaagttaa
       61 ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc tgctgggcta
      121 actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg ttaaaacagg
      181 gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat acgatatcct
      241 gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc gcaggttgat
      301 gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct aataaaaatg
      361 ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca agagatgaat
      421 aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta gtgggtattt
      481 tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact tctctgaccg
      541 gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc actcctgagg
      601 gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac ttttggctgc
      661 ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca ttgatatagt
      721 taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct tgtaattgaa
      781 ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca ttcctgccgt
      841 tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac agagactcac
      901 gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg cttttgggtt
      961 cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg ctgctgtttt
     1021 caatccacga attgaccaga aagagctcat tggtatggct atttttaatc cataccgcag
     1081 tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc cctccggcag
     1141 catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac tttattttag
     1201 tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt ataaagaaat
     1261 gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc ggcatgatac
     1321 aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac cggtacatta
     1381 ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat tcgtatctca
     1441 taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg gtgcctttat
     1501 aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg gttgagaagc
     1561 tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata ccgacacccg
     1621 ttgctgttgt tgtatttgta ctggatacaa tggccaataa attatcattg attgcatcag
     1681 cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag tcgttcaact
     1741 gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata tcggtacgat
     1801 attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag gcagcattca
     1861 ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa ctacccgtag
     1921 cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata tatattcctt
     1981 caatctcatt tatcgactcc acatccgtat ataaccgatt actttattta agacactgat
     2041 agtagtaaaa ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc
     2101 taaaaaatag agagccccgt tagatgaata cttccgcgca aaatatattc accacaaata
     2161 tagacctgaa gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt
     2221 aaattatctt aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg
     2281 tacgaaaaat gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa
     2341 gaggtcgctt cacgcgataa atctgaaacg aaacctgaca gcgcgccccg cttctgacaa
     2401 aatagacgca tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa
     2461 gattattgac ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca
     2521 ggctcgtatt gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa
     2581 ccctgcatcg caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt
     2641 ctcccgctct tatgacctcc cggaaaccac cagcgaagcg gagctgctgg agcttatcga
     2701 tgcgctgaat gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg
     2761 tattgataac gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca
     2821 tccttacaac gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg
     2881 cggtatcgtc acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt
     2941 gattggcgca tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc tcgcaggttg
     3001 caccaccacc gtgactcacc gcttcactaa aaatctgcgt catcacgtag aaaatgccga
     3061 tctgttgatc gttgccgttg gcaagccagg ctttattccc ggtgactgga tcaaagaagg
     3121 cgcaattgtg attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt
     3181 cgtgtttgaa gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg
     3241 cccgatgacg gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga
     3301 tccacagggt gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt
     3361 gcgacttgct gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg
     3421 ccgaaggcca ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg
     3481 ccggtcagac agtgagtttt gcaggacaca gcgtacaggt tgttgcctga tccctttccc
     3541 ggcctgtatc aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc
     3601 atgctgatag catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc
     3661 cgccgttccc ctttgcattg gtttagggtt gggttcgaaa ggtctgttat ttgccggaat
     3721 catcctggcg atgctgcccg atgccgacgt attgtcgttt aaatttggcg ttgcttacgg
     3781 caatgttttt ggtcatcgcg gttttaccca ttcgctggtg tttgcgtttg ttgtcccgct
     3841 cttatgtgtg ctgattggac gtcgatggtt cagggctggg ctgattcgct gctggctgtt
     3901 tttaaccgtc tcgttgctat cgcacagctt actggattcg gtaaccactg gcggtaaggg
     3961 cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc aggtgattaa
     4021 agtcgcgccg tttgcattgt ctcgttacac cacagcgtac gggcatcagg tgattatttc
     4081 cgaattgatg tgggtgtggt tgccggggat gctactgatg ggaatgttgt ggtggcgcag
     4141 acgataaccg gatgcgaaaa ctcgcatccg gcaattgcgc aattacttac gacgccaggt
     4201 ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt cacgtgccgc
     4261 atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa ttaacgcttc
     4321 aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt tgcaggaacg cttccggttc
     4381 ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca ttgcattcgc
     4441 cgctgccata tcttctgctt tcagacggtt cacttcacgc gccatatcaa acagcacgga
     4501 ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg cttcaaacgc
     4561 ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcagtgt agaggcgctc
     4621 cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcagctggc tgcgatagtg
     4681 gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca catcgcgcac
     4741 ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca ccattccgga
     4801 gtgcatccag tagttcacat actgaccatc atgggcacag gtggactgcg cgatttcgtt
     4861 ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt ggttacccag
     4921 ctgcttgcag ttcatggccg aacattcaat gtgccagcca ggacgacccg cgccccacgg
     4981 agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt ccatccggtt
     5041 gcgtttgtca tcaaccacgt caacgcgcgc gccagcctgc agctggtcga gatcctgacg
     5101 cgacagcacg ccataagttg gatcggtcgg gacgtcgaac atcacgtcgc tgttgtccgc
     5161 cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt ctgcgatatg
     5221 gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa aatctttgtg
     5281 catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt cattggcgcg
     5341 tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt tatagccgag
     5401 gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt gaccgatatg
     5461 acagagatcg taaacggtga ttccacacac gtacatgccg acttccccgg cgtgaatagg
     5521 cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg aagattccgt
     5581 ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg cgaatcagca
     5641 tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat atatgacccg
     5701 aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact ttccacacca
     5761 atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca gttaaaaact
     5821 tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt gttatcaacg
     5881 gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc accaaagaac
     5941 cgatcaaaaa cgaagccaac aacggcctga aaaatacccg tggtacgctg gcaatggcac
     6001 gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt gataacgact
     6061 tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt gctgaagtgg
     6121 ttgacggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt aacggtatgc
     6181 accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc gagtaatcgt
     6241 ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga tcaccgccgg
     6301 ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata ttcttggcga
     6361 tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc agatggcggc
     6421 agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg gcaaccgtga
     6481 ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc cggaagaaaa
     6541 ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc tgtgcaccga
     6601 tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc aaatgctatt
     6661 cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgcg cgaacagcaa
     6721 agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg ctgtggtcag
     6781 tgcgatggaa aaacatcagg tgcaatggct aatccacggg catacccatc gcccggcggt
     6841 gcatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactgggtg cctggcatac
     6901 ggaaggttcg atggtgaaag tcacggctga tgacgttgag ctgattcatt ttccgtttta
     6961 aaaaacccac aactttgctg atttcacagc cacgcaaccg ttttccttgc tctctttccg
     7021 tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt taagacgcat
     7081 gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa gcgactgggc
     7141 taccatgcag ttcgccgtcg aaatcttcga aatcctgaat gtcccgcacc acgttgaagt
     7201 ggtttctgct caccgcaccc ccgataaact gttcagcttc gccgaaagcg ccgaagagaa
     7261 cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgccag gcatgattgc
     7321 cgccaaaacg ctggtgccgg tgctgggcgt gccagtacag agcgccgcac tgagcggtgt
     7381 cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta cgctggcgat
     7441 tggtaaagct ggcgcggcaa acgcggcatt actggcagca caaattctcg cgactcacga
     7501 taaagagcta caccagcgtc tgaatggctg gcgcaaagcc cagaccgacg aagtgctgga
     7561 aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa cgggcagtta
     7621 ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg ctgtctggcc ggtcgggctg
     7681 gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga gatcgaacgc
     7741 tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt gaaccgcgat
     7801 gtgttcccga ttattgctga ccgtctgact cagaaacagc ttttcgataa gctccacctg
     7861 ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt gtttgatcgt
     7921 ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg cggtcaatgg
     7981 agtttacgcg ccaatgaaac cgaacagtta ccggcagagt gttacggtga atgtattgtc
     8041 gagcagggca ttaacttctc tggtgaagta tcgctggttg gcgcgcgcgg ctttgatggc
     8101 agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt gcgcaccagc
     8161 gtcgcttttc cgcaggccaa cgcacagcag caggcgcaag ccgaagagat gctgtcggcg
     8221 attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt caccccgcaa
     8281 ggtctgctga tcaacgaact ggctccgcgt gtgcataaca gcggtcactg gacacaaaac
     8341 ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct gccgttaccg
     8401 caaccagtag tgaataatcc gtcggtgatg atcaatctga ttggtagcga tgtgaattat
     8461 gactggctga aattgccgct ggtgcatctg cactggtacg acaaagaagt ccgtccgggg
     8521 cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac tgcgacgctg
     8581 gaagccttaa tcccgctgct gccgccagag tatgccagcg gcgtgatttg ggcgcagagt
     8641 aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc cttatccggc
     8701 ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc atcaggcatg
     8761 gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc gcttcgcccg
     8821 ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacta cggcttctga
     8881 cataaccact caccgccgtt accttcggcc ccatcgaacc atcggctttg gcaaatggcg
     8941 ctaactcatc cggtgtggca tggcgaatgg cacgttgctg cggcgttccc cagttttcat
     9001 ataccgcatc agcatcggtg aggatcacca gtccatcggc attaatctgc tcggcgagca
     9061 acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct tccgtcacag
     9121 gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac aactcgatgg
     9181 cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc aaatatttac
     9241 catcacgttt catctgccag ccgtaagccg cttccagtgc ctcttgttct tctggctgat
     9301 aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc gaaacctcaa
     9361 tgcgcgtcag caccgtcgta acaggcggca tctgcggctg tgcgctcaag ctctgcgcca
     9421 gcatatagcc aatcatcccc tggctttccg ccaccagtac atccagcgga tacggttcta
     9481 cctctttcca cgccagattc tgtaacgcca gcagccccac ctgcggcccg ttgccgtgaa
     9541 caatcgccag ccgataagaa cgggccaggc gtgccagcgc gggtacagca ctggcgatat
     9601 tgcgatattg attttccgcc gtcagcgcct caccgcgctg gagtaaggcg ttgcccccaa
     9661 gagctacaac cagtgttttc atggttttcc ttgtaataat tgttggccaa gccagaaccc
     9721 cagcagcgta tctgcccctg acgtatgccc cagcgccagc agcgaatcaa tcgcaacagc
     9781 ggtacgtttc gggcaactca gagcatggac aaagtgcagg agtggcgagg cgaaatatcc
     9841 ttgcgcggca taacgtaaat aactgacgct caccgctgtg gtaacgagtt gaagattgtc
     9901 ggaacaggca aaaaacgggc gaccggcgcg cgcatctaaa gcgccataat accaggccgc
     9961 cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccggggc ctttacccag
    10021 ccagtgccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt gacgaaactg
    10081 gcgtaattca gcgggcagcg gatcgcttgc cgccatcgcc agtggcccga aaagtccggt
    10141 ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgaag gcggcgtaat
    10201 acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc gaatcccttg
    10261 agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat cgaattgcgt
    10321 gcggcgaaat tacacattcc cgtcaagcta tagaagaaac gctgccctgg ctttcctctt
    10381 ttggcgctga cccaacgggt gggatgaccc gtttacttta ttcgccggaa tggctggaaa
    10441 cccagcagca atttaaaaaa agaatggcag caagtgggct ggaaacacgt ttcgatgaag
    10501 tggggaattt atacggtcgc ctgagtggca ccgaatatcc acaggaagtg gttctgagcg
    10561 gttcgcatat cgataccgtg gttaacggcg gtaacctcga cgggcaattc ggcgcgctgg
    10621 cggcgtggct ggcaattgac tggttgaaaa cgcaatacgg cgcaccgtta cgtacggtcg
    10681 aagtggtggc gatggcagaa gaagaaggca gccgcttccc gtatgtcttc tggggcagta
    10741 aaaatatctt tgggctggcg aatcctgacg acgtgcggaa tatctgtgat gccaaaggaa
    10801 atagttttgc cgatgcgatg aaggcttgcg gatttactct gccgaacgcc ccactaactc
    10861 cgcgtcaggc tataaaagcc tttgttgaac tgcatattga acagggctgt gtgctggaaa
    10921 gtaatgggca atcaattggc gtggtgaatg caattgtcgg gcagcgtcgc tatacggtga
    10981 cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt cgtgatacgg
    11041 tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag atgggcgatc
    11101 cgctggttct gacctttggc aaagtagagc cgcgcccgaa tacggtaaat gtggtgccgg
    11161 gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc tgccgtgctg cgcgatttca
    11221 cccaacagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt ggtattgata
    11281 tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggagctggtc gccaccctga
    11341 cagaattgtg tgaaagagaa aaactgaatt accgggtgat gcacagtggt gccgggcacg
    11401 acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat ttttatcccc agcatcaacg
    11461 ggatcagcca taacccggcg gaacgcacca atattactga ccttgccgaa ggggtcaaaa
    11521 cgttggcact catgctttat caacttgcct ggcaggaata aggagtcata aatgggatat
    11581 ttaaataacg tcaccggtta ccgcgaagat ttactggcta accgtgcgat tgttaaacac
    11641 ggtaatttcg cactgttaac cccggatggt ctgataaaaa atattattcc ggactttgaa
    11701 aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt tgattatctg
    11761 gtcacactgc atcaaaacgg tggcaaccaa cagggcttcg gtggcgaagg cattgaaacg
    11821 ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac atttgcctta
    11881 agcgaaggtg gctatcttta ttgcccacca ggctccttaa tgacgtttgt taacgcccag
    11941 gccgaagaca gccaaatctt tttatataag cgccgctatg ttccggtaga aggccatgca
    12001 ccgtggctgt tttctggcaa tgccagcgaa ctggaacgca ttcactatga aggtatggat
    12061 gatgtgatcc tgctggattt tctgcccaaa gagttaggtt ttgatatgaa catgcatatc
    12121 ctctcttttg caccaggtgc cagccacggt tatatcgaaa cacacgttca ggaacatggt
    12181 gcctatattc tttccggtca gggggtttat aa
//
LOCUS       DAJTRN010000108        12047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRN010000108 DAJTRN010000000
VERSION     DAJTRN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            104..187
                     /locus_tag="OO328_003270"
     CDS             104..187
                     /locus_tag="OO328_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5931723.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(241..1593)
                     /gene="gorA"
                     /locus_tag="OO328_003271"
     CDS             complement(241..1593)
                     /gene="gorA"
                     /locus_tag="OO328_003271"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5931724.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(1665..2507)
                     /gene="rlmJ"
                     /locus_tag="OO328_003272"
     CDS             complement(1665..2507)
                     /gene="rlmJ"
                     /locus_tag="OO328_003272"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5931725.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            2710..4752
                     /gene="prlC"
                     /locus_tag="OO328_003273"
     CDS             2710..4752
                     /gene="prlC"
                     /locus_tag="OO328_003273"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5931726.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            4760..5512
                     /gene="rsmJ"
                     /locus_tag="OO328_003274"
     CDS             4760..5512
                     /gene="rsmJ"
                     /locus_tag="OO328_003274"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5931727.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5561..7030)
                     /gene="dtpB"
                     /locus_tag="OO328_003275"
                     /pseudo
     CDS             complement(5561..7030)
                     /gene="dtpB"
                     /locus_tag="OO328_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7348..7782)
                     /gene="uspA"
                     /locus_tag="OO328_003276"
     CDS             complement(7348..7782)
                     /gene="uspA"
                     /locus_tag="OO328_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5931728.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8176..8511
                     /gene="uspB"
                     /locus_tag="OO328_003277"
     CDS             8176..8511
                     /gene="uspB"
                     /locus_tag="OO328_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5931729.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8582..10081)
                     /gene="pitA"
                     /locus_tag="OO328_003278"
     CDS             complement(8582..10081)
                     /gene="pitA"
                     /locus_tag="OO328_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5931730.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10313..11512
                     /locus_tag="OO328_003279"
     CDS             10313..11512
                     /locus_tag="OO328_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5931731.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         2949 a         3049 c         3215 g         2834 t
ORIGIN      
        1 tggccggagt gagattgccc acatctaaca atgaggcatg cgccgtgaca ggcagtggat
       61 gagtaagcgg atgcattctc actccatcgc atggagaaaa cgggtgattg ataaagcaat
      121 catcgttctt ggggcgttaa ttgcgctgct ggaactgatc cgctttctgc ttcagcttct
      181 gaactgatag cggaaacgta attaagggct aagagcacac tactcttagc cctttaacat
      241 ttaacgcatg gtcacgaact cttctgccgc cgttgggtga atggcgacgg tattgtcgaa
      301 gtcttttttg gttgcgccca tcttcagcgc caccgcgaag ccctgcaaca tttcgtccat
      361 accaaagcca atgccgtgaa taccgacaat cttctcttct ggcccaacgc acaccagctt
      421 catgcggcac ggctggcggt gagtggtgac ggcggtatac atcgcggtga aagaggattt
      481 atacactttc acctgatcgt cgccgtactg ctcgcgcgcc tgcggttccg ttaaaccaac
      541 agtaccaatc ggcggatggc tgaagaccac ggtcggaatg ttgctgtaat ccagatgctc
      601 atccggcttg ttattaaaca ggcgttcaga gagacggcga cccgctgcaa ctgccaccgg
      661 tgtcagttcc actgcacccg tgttatcgcc caccgcgtaa ataccttcaa tattggtgtt
      721 ttgatattta tcgacgacga tatagccttt ttcgttggtt ttaacgccag cggcttcgag
      781 gttgatgttg tcattggcag gctcgcgacc aatcgcccaa atcaggcaat ccaccgtttc
      841 actgcgacca tcttccagct ccagcgtcag gctaccatcg gcatttttca ctaccgcttt
      901 cgggatggcg ttggtatgca actgtgggcc ttcggcgttc atcacttcga ccagcgtttc
      961 agagatcatc gggtcgaagc tgcgcagcgg cgcatgttta cgcacaaaca gatgcgtttt
     1021 cgcgccgagg ccgttaatca cgccagccag ctcaacggcg atgtaacccg cgccaacaac
     1081 cgccacgcgc tctggcaaag caggaagggc gaagaagcca tcggaatcaa taccgtattc
     1141 cacgcctgga atatccgggt ggctcggacg accgccggtg gcgatcagaa tatgatcggc
     1201 cgtgatggtt tcgccgttaa cttccagcgt tttggcatca acgaagcggg caaagccttt
     1261 gattacatca acgttatttt taccgagcac gttttcatag gaagtatgaa tacggtcgat
     1321 ataggcggta cggctggcga tcaacgtttc ccagttgaac ttgttgatgg tggtatcgaa
     1381 accataatca gggccgtaca tatggatcgc ttcacggatt tgcgccgcgt gccacatcac
     1441 ttttttcggc acgcagccga cgttcacgca ggtgccgccc agctctttgg cttcaatcag
     1501 tgcgcatttc tggccgtaca tagccgcgcg gttgatggag gcgataccgc cgctgccgcc
     1561 gccgatggcg atgtaatcat agtgtttagt catgacatat tgtccttatc gttgattacc
     1621 gcgattgtag cgcgagacgt aataggtgcc agcaatggct gcaattactc cggcacgatc
     1681 cagcttacgg tggcgtgccc ggtacctgcc ggaaccagtt tgctgtgcag ccacgacagc
     1741 acgttattca tctgctgttc cagcttccac ggcgggttaa tcacaatcat gccggaagcg
     1801 gtcatgccac ggcgatcgct gtctggcagc accgccagtt caatttgcag aattttgcga
     1861 atgccggtcg cttccaggtc gtggatcatg cgcttaattt gctggcgcag caccaccgga
     1921 taccacaatg cgtaagtgcc agtggcgaaa cgtttgtaac cttctgctat cccgctgacc
     1981 accgcctgat agtcggtttt catttcatac ggcgggtcga taaggattaa accacggcga
     2041 gaaaccggcg gcagtttggc cttaagttgc tggaaaccgt cggctttttc gacgcgtgca
     2101 cggctgtctt tctgaaatcc agaacgcagc aacgggtaat cgctcgggtg cagttcggtc
     2161 agttgcaggc tgtcttgttc gcgcagtagc tggcgagcaa tcaacggcga accggggtag
     2221 taacgcaact ggccgctacg gttgaagtgt tttaccacat tgatgtacgc ctccagttct
     2281 gcgggcaaat cgtcctgctg ccagatacgg gcgatacctt cgagatattc gccggtacgc
     2341 tcggcatgtt cgctacctaa ctgataacgc ccggcccctg cgtgggtgtc gagatagaga
     2401 aacggtttat ctttctcttt cagcgactcg atgatcaggc tctgaacggt atgtttaagg
     2461 acgtcggcgt ggttgccagc gtgaaagctg tggcgataac tgagcatggg taaaggtgtt
     2521 ccggtaagta aaaattggcc cgtattcggg gcgcacaaaa gcctatcagg acagtatacc
     2581 gaaaagaggc cgccgccgcg aaagcgtaac gtttctcatt gaaattcact acacttaacc
     2641 ccatgctaca cacattatgt aaagcgcctg ttgagcgctt ccttaacctc tttaaccagg
     2701 actgcgctaa tgacgaatcc gttactgact ccctttgaat tgcctccgtt ttctaaaatt
     2761 ctcccggaac atgtcgttcc agccgtgact aaggcattga acgactgccg cgaaaatgtg
     2821 gagcgcgtag tagcgcaagg ggcaccgtac acctgggaaa atctctgcca gccgttggcg
     2881 gaagtggacg atgttttggg gcgtatcttc tccccggtca gccacctgaa ctcggtgaaa
     2941 aatagcccgg aactgcgtga agcgtacgaa caaaccctgc cgctgctgtc ggaatacagc
     3001 acctgggtag ggcaacatga agggctgtat aaagcgtacc gcgacctgcg cgatggcgat
     3061 cattacgcca cgctgaacac ggcgcagaag aaagcggttg ataacgccct gcgtgatttt
     3121 gaactctctg gcataggtct gccgaaagag aaacagcagc gttacggcga aattgctacc
     3181 cgtctttctg aactgggcaa ccagtacagc aacaacgtcc tcgatgcgac gatgggctgg
     3241 accaaactcg ttaccgacga agcggggctg gcggggatgc cagaaagcgc gctggctgcg
     3301 gcaaaagccc aggccgaagc gaaagagctg gaaggctacc tgctgacgct ggatatccca
     3361 agctacctgc cggtaatgac ctactgcgac aaccaggctc tgcgcgaaga gatgtatcgc
     3421 gcttacagca cccgcgcctc cgatcaaggc ccgaatgccg gtaagtggga taacagcaag
     3481 gtgatggaag agatcctcgc gctgcgtcac gaactggcgc aactgctggg ctttgaaaac
     3541 tacgccttta aatcccttgc cactaaaatg gcagaaaacc cgcagcaggt gctggatttc
     3601 ttaaccgatc tggcaaaacg cgcgcgtccg caaggcgaaa aagagctgga gcaactgcgt
     3661 gccttcgcta aagccgaatt tggtgtcgat gagttgcagc cgtgggatat cgcttactac
     3721 agcgaaaaac agaaacagca cctctacagc atcagcgacg agcagctgcg tccgtacttc
     3781 ccggaaaaca aagcggttaa cggcctgttt gaagtggtga aacgtattta cggcatcacc
     3841 gctaaagagc gtaaagatgt tgatgtctgg catccggatg tacgtttctt cgaactgtat
     3901 gacgaaaata acgaactgcg cggcagcttc tacctcgatc tgtatgcccg tgaaaacaag
     3961 cgcggcgggg cgtggatgga tggctgcgta ggccagatgc gtaaagctga tggttctctg
     4021 caaaaaccgg tcgcgtattt gacttgtaac ttcaaccgcc cggtaaatgg taaaccggcg
     4081 ctgttcactc acgacgaagt gatcaccctg ttccacgagt tcggtcacgg cctgcaccat
     4141 atgctgaccc gcatcgaaac cgctggtgtt tccggtatca gcggtgtgcc gtgggatgcg
     4201 gtcgaactgc cgagtcagtt tatggaaaac tggtgctggg agccggaggc gctggcgttt
     4261 atctctggtc actatgaaac cggcgaaccg ctgccgaaag agttgctgga taaaatgctg
     4321 gcggcgaaga actaccaggc ggcgctgttt attctgcgtc agctggagtt cggcctgttt
     4381 gatttccgcc ttcatgccga gttccgcccg gatcaggggg caaaaatcct cgaaactctg
     4441 gcagaaatca agaaactggt tgccgtggtg ccatctccgt cctggggccg tttcccgcac
     4501 gctttcagcc atattttcgc cggtggttat gcggcaggtt actacagcta cctgtgggct
     4561 gacgtactgg cggcagatgc tttctcgcgc tttgaggaag agggcatttt caaccgtgaa
     4621 accgggcagt cgttcctcga caacattctg agccgtggcg gttcagaaga gccgatggat
     4681 ctgttcaaac gcttccgtgg tcgtgaaccg cagctggatg cgatgctgga gcattacggc
     4741 attaagggct gatcattcag tgaaaatctg cttaattgat gaaacaggcg ccggagacgg
     4801 cgccttatct gttctggcgg cccgctgggg gctggagcac gatgaagaca acctgatggc
     4861 gctggtgtta acgccggaac atctggaatt gcgcaagcgt gatgagccaa aacttggcgg
     4921 catctttgtt gattttgttg gcggagcgat ggcgcaccga cgcaaattcg gcggtggtcg
     4981 cggtgaggcg gtggcgaaag cggtgggcat taaaggcgat tatttgccgg atgtggtgga
     5041 cgccaccgca gggctggggc gcgatgcctt tgtactggct tcagtcggct gccgcgtgcg
     5101 gatgctggag cgtaatccag tggttgccgc gctactcgac gacggcctgg cgcgtggtta
     5161 tgcggatgcg gaaatcggcg gctggttgca ggagcggttg cagttaattc acgcctccag
     5221 cctgacggcg ctaaccgata ttaccccacg cccgcaggtg gtctatctcg atccgatgtt
     5281 cccgcataag cagaaaagcg cgctggtgaa aaaagagatg cgtgtctttc agtcgctggt
     5341 ggggccggat cttgatgccg atggattact ggagcctgca cgcttgctgg cgaccaaacg
     5401 cgtggtggta aagcgcccgg actacgcgcc accgctggcg aatgtcgcca cgccaaacgc
     5461 ggttgtgact aaagggcatc gctttgatat ctatgcaggt acgccagtgt aaaagtaaag
     5521 cagggtagcg ataacgctac cctgctttct gcaagaataa ttaatggctt tccggcgtcg
     5581 caatcatgcg tttcagccac ggcaccatca acagcatcac tactgcaacg cccagcgtga
     5641 ccagaccaat cttaccaaac acgttggtat agacgggcaa cgtctcaagc ggatcggtaa
     5701 tgttgtccgg caccgcggta aatgttgcca catagccgcc cagcaagaac gcggcagcct
     5761 gcgtcaggaa ccacatcccg agaataaagc ccatcaaatg ctgcggcacc agggcagcaa
     5821 tcatcgccag gccaagggcg ctaataaaca attcacccag gctctagaat aaatacacca
     5881 gcacgataaa ccatggcgat gtcagcccct ggtcatccgc aaaccacatt cccgcagcag
     5941 cagccgtcaa aaagcccagt gagcacataa acatgctgag agtaaatttc atcggcatcg
     6001 agaggtcttt gcctttgtta cccagatgcg tgtaaatgcc tgccagtatt gggctggcga
     6061 gtaccaccca gaacgggtta agcgcctgga agctgaccgg gttgatggaa aaaccgagaa
     6121 tttcatgatg cacgttgttg atggcaaaga agttcagcga tgttggcatc tgggcgtaga
     6181 gaatgtaaaa caccaccgct tcgagcatca ggacaaaggc gacaaacatt ttattgcgcc
     6241 cggttttatc cagcttgaat gcctgacgaa agaagatgat agtgacgacg atggagagaa
     6301 caatcagcac cagattggcg acttctacgt tgtgcatcag ccatgcacat acgaagatca
     6361 tcaccacgct gccaagtaat acgtacaaca gtttgctgaa gctcatcggc ttgaagtcgg
     6421 gttcagaacc aatgtctttc accattccac gacaggcgat gtaaaccagt aatgcgataa
     6481 ttaaccccgc cccgcacagg ttgtaggtga ctgaataacc gaatctatca gcgatcacag
     6541 gggccagcga taacgctatc aacgagccga tattgatcga catatagaac agggtgaatg
     6601 cgccatcaag ccgcggatct ttcggcggat agcacttcga aagcaagctg gctgggttag
     6661 ctttaaacag gccgttaccg acagcgatag tccccagggc gatgaaaatc aggtcaggct
     6721 taagtagcga catgccggtc atgaagtagc caatcgccag cacaagtgct ccaagaacaa
     6781 tggtgcgttt ggtccccaac aggtggtcgc cgacatagcc gccaatggaa atgaggccat
     6841 agaccagtgc agcaaaagca ccaaaagtga caaaagcctg ctcttgcgag aatccaagct
     6901 gtttaacgaa gaaaaccgcc agtacgccct gcacgccgta gtagccgaat cgctcccata
     6961 actcgacaaa aaagatcatg aaaaatgggc gaggttgctg cagcatcccc atgggtgttg
     7021 ttgtattaat atttattaac cttccaatac catcctgaaa agtatgaacg cgtaagggat
     7081 aacgcgcgaa aaagtttaca tctgtcagag attcttaata ttgatgcggt gaatagatta
     7141 atacactatt acaacagaaa ataaccagct gatcacacta atgatggaaa atatgatggt
     7201 gttaaatttt gtattggcag gagattttga ttgcgtacgc agatcgtagg ccagataagg
     7261 ggtttacgct gcatcaggta acaaggcgcg ccaaaaagca aaacgcccgc atatgcgggc
     7321 gttcaggaac acgtcgtgga gggatgatta ttcttcttcg tcgcgtagcg gaacaatcag
     7381 catatcaacg tgaacggtgt tgatcagctg acgtgcggaa gacatcagtt tgctccagaa
     7441 gtcctggtgg tgaccacaaa ccaccagatc catatcgtat ttcttgattg catcgaccag
     7501 aacctggcct aggtcgccgc tgccgctcag ggtttcagtg attggatagc ctgcattagt
     7561 ggaaagctcg gtcagcgcat gatgtgtctc ttcagagatg cgtttctgca tatcacccag
     7621 attcacatca ataagcccgg tgtataggtc agagtagttt acatctacgt ggatcagaga
     7681 aactttcgca ttgtaggggc gagccataga gactgctttc tctaccagaa ctttgctttc
     7741 cggggagaga tcgactgcga tgagaatgtg tttataagcc atagtgttac tccttccata
     7801 aagttgtcga tgactggcca gctagcgttt cttgtgccgt tacgtcaccc gcgtcctgcg
     7861 aactacgtgc gcggggctaa cccagccgta aagattcagt gttaagacca tccttacctt
     7921 atagcgaccc ggatgatccg tcaatccgcc ttgcttaccc gttagataaa caaattttcg
     7981 acatgaatat tatattgata gtggttaacc ttctggaaaa aaaacaacct gatctcctac
     8041 actatctata gagccgctcg gatgttggtc accacaaaag cggtttctcc aggacctggt
     8101 gttaactgat tggcagcgta tcgggagcgg tagccccggg gtggaactcc gcgggcaggt
     8161 cgccggggag gaggtatgat aagcaccgtc gcattatttt gggctttatg tgtcgtttgc
     8221 attgttaaca tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt actgcgtaac
     8281 tgcgatccat tgctctatca atatgttgat ggagggggct tttttacctc acatggccaa
     8341 cccaacaaac aggtgcgtct cgtttggtat atctatgccc aacgttatcg cgatcatcac
     8401 gatgatgagt ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct gactagcgca
     8461 ttgtgtggtc tggtggtggt cagcctgatt gcattgatga tttggcatta agcacttcag
     8521 caataataac gtataaaaaa agcgggccag taaactgacc cgctcgtttt ggtgcgtacg
     8581 attacaggaa ctgcaaggag aaccagtaca gcccgccgga aagtagtacc gcagccggaa
     8641 gggtaaacac ccaggccatc agaatactgg ttacggtttt acgctgtaag ccgccgccat
     8701 ctaccaccat cgtccccgcg acagaagagg agagtacgtg agtggtggaa accggcatcc
     8761 cggtataact cgccaggccg attgacactg ccgccgtcat ctgggcagac atcccctgag
     8821 cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca cgacgccagc
     8881 caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac accggcgcat
     8941 actcgatggt gctaagcatg tcggacttca gtttcttcaa caggcgctga tcgtcagcac
     9001 tcacaccagg catcttcacc actttgtcga tagtgtcaga aacgcacagc ataatgcggc
     9061 gcatctggct acgttgatca agcgacagct tgtcgtagct ttccacatcg gtggtcaaca
     9121 tacctttcag gcggttgagc gcgttaatgg tattcgacgg atggcagtgg aactccgcag
     9181 gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta gcctgtttga
     9241 gcagcgcagg atgctgctca aagtaagctt cgacgttgtt gatggcatca cgggtacggg
     9301 tgatttcgta gccagtggca ttcatgttca ccacgaagcc tgctggcgcg acgccaatca
     9361 ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca
     9421 cgccgatagc ggaaaggatc agcgcgatac gcgtccagaa cggcggcttt ttcttgccgt
     9481 ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc
     9541 agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca ataggggaaa
     9601 cgatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc accactgacg
     9661 tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc agcgtatgag
     9721 agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc gccgccagca
     9781 acatagagaa caccatggca aggccatgag acgatcccat attaagcagc agatccgtcg
     9841 gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca cccaaaaagt
     9901 tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta tagataacgg
     9961 ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac agcacaaaag
    10021 ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac aaatgtagca
    10081 taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg
    10141 cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt cactcaaaaa
    10201 ataattatct tatataattc aggcaaatac ttccttttag taatattgat gctggtgcga
    10261 ccactgagga atctttacaa ttcacgcccg ttttttctaa gaggagcgca acgtggaaag
    10321 gtttgatgcc attattatag gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg
    10381 tcaggtagga cgccgggttc tgctgatcga taatggtaaa atactagggc gcaaaatcct
    10441 tatgtctggc ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct
    10501 gagccagaat ccgcattttt gtaagtctgc actcgcacgt tttacccagt gggatttcat
    10561 tgatctggtc aataaacacg gtatcgcctg gcacgagaaa acgttagggc aactcttctg
    10621 cgatgactcc gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt
    10681 aaccttcaga ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct
    10741 tgatctgaac ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc
    10801 aatgccgggg ctgggcgcat cgccgtttgg ttataagatt gccgaacaat ttggcctcaa
    10861 cgtgctgccg acccgcgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga
    10921 gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcaccgt
    10981 tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg tgttgcagat
    11041 ttcaagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggacct
    11101 cgaaaccttc ctgaatgagc agcgtaacgc acatccgaat caaagcctga aaaacacact
    11161 ggcggttcat ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga
    11221 tgtttcgcta aaacagctca acgtgtgtga ccaacaggca ctgattagca cattgaccga
    11281 ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg
    11341 cgtggacacc aacgaacttt cttcacggac gatggaagcg cgcaaagtgc ctgggctgta
    11401 cttcatcggt gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc
    11461 gtggtcgagt gcatgggctt gtgcgcagga tttggcgtca attgtgaaat aaccatgacg
    11521 ttttcccgtc agccgtttgt atatgtcgtt attaagtcat tttggtacgg ttctgcatcc
    11581 gacattctgg tgttaactcg ctgttttgtt agcgaattaa cgctattgcc gttgtggtta
    11641 ttttatagtg tatttattct cagagaatat atattactga aataaaaaac acagagaata
    11701 accaaaggaa attatttgaa acgtaaaagg tatatatttt taatctgtaa aatttaaaat
    11761 cacctcctct ggattgaatc aacttgctct atttaagacg attgttttta cattttcacc
    11821 acctgtatca ggtggtgttt ttttattggc ggcaatgcct ttatcttatt tttattacgt
    11881 tgtaaatatg gctattccta aatgaaattt gatttgctca gtttatttgt gatgtcaggg
    11941 gagtgcttgc catgagagtc gtgatattat tattcatcat ttttaggtaa tgctgccaac
    12001 ttactgattt agtgtatgat ggtgatttta aggtgcttgc gtggctt
//
LOCUS       DAJTRN010000109        11925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRN010000109 DAJTRN010000000
VERSION     DAJTRN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..2342)
                     /locus_tag="OO328_003280"
     CDS             complement(<1..2342)
                     /locus_tag="OO328_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5931732.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO328_003281"
     CDS             complement(2276..2899)
                     /locus_tag="OO328_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5931733.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO328_003282"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO328_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO328_003283"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO328_003283"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5931734.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEYIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO328_003284"
     CDS             7507..8229
                     /locus_tag="OO328_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5931735.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO328_003285"
     CDS             complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO328_003285"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5931736.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10301..11896)
                     /locus_tag="OO328_003286"
     CDS             complement(10301..11896)
                     /locus_tag="OO328_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5931737.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2759 a         3307 c         2943 g         2916 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgta ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg ttataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcgg
//
LOCUS       DAJTRN010000110        11902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRN010000110 DAJTRN010000000
VERSION     DAJTRN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(247..891)
                     /gene="cheZ"
                     /locus_tag="OO328_003287"
     CDS             complement(247..891)
                     /gene="cheZ"
                     /locus_tag="OO328_003287"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5931738.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(902..1291)
                     /gene="cheY"
                     /locus_tag="OO328_003288"
     CDS             complement(902..1291)
                     /gene="cheY"
                     /locus_tag="OO328_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5931739.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1306..2355)
                     /gene="cheB"
                     /locus_tag="OO328_003289"
     CDS             complement(1306..2355)
                     /gene="cheB"
                     /locus_tag="OO328_003289"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5931740.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2358..3218)
                     /gene="cheR"
                     /locus_tag="OO328_003290"
     CDS             complement(2358..3218)
                     /gene="cheR"
                     /locus_tag="OO328_003290"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5931741.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3237..4838)
                     /gene="tap"
                     /locus_tag="OO328_003291"
     CDS             complement(3237..4838)
                     /gene="tap"
                     /locus_tag="OO328_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5931742.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4884..6545)
                     /gene="tar"
                     /locus_tag="OO328_003292"
     CDS             complement(4884..6545)
                     /gene="tar"
                     /locus_tag="OO328_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5931743.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6690..7193)
                     /gene="cheW"
                     /locus_tag="OO328_003293"
     CDS             complement(6690..7193)
                     /gene="cheW"
                     /locus_tag="OO328_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5931744.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7214..9178)
                     /gene="cheA"
                     /locus_tag="OO328_003294"
     CDS             complement(7214..9178)
                     /gene="cheA"
                     /locus_tag="OO328_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5931745.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9183..10109)
                     /gene="motB"
                     /locus_tag="OO328_003295"
     CDS             complement(9183..10109)
                     /gene="motB"
                     /locus_tag="OO328_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5931746.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10106..10993)
                     /gene="motA"
                     /locus_tag="OO328_003296"
     CDS             complement(10106..10993)
                     /gene="motA"
                     /locus_tag="OO328_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5931747.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11120..11698)
                     /gene="flhC"
                     /locus_tag="OO328_003297"
     CDS             complement(11120..11698)
                     /gene="flhC"
                     /locus_tag="OO328_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5931748.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11701..>11902)
                     /locus_tag="OO328_003298"
     CDS             complement(11701..>11902)
                     /locus_tag="OO328_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5931749.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2720 a         3344 c         2893 g         2945 t
ORIGIN      
        1 caccatgaac tgacgaaaaa acgttatgaa gtatggcatc actacgatgt actgcactac
       61 cagtcatcgt cattttctcg ttcacaattt aatgcattga taaatttact ggcgcaacaa
      121 tcacgcttcg ctgatctaaa attatcaaac tacaaataac catcctccac gtactccctg
      181 aacgataccc gccaccctgc tatcggcttg gcatcatcgc gcaggccgaa gttcaggcaa
      241 tacaaatcaa aatccaagac tatccaacaa atcgtccacc tgatcctgac tggctaccac
      301 gccggctttg ctggtatcga cctgaggtcc attaagcaaa ctctggtttt cacgttttgg
      361 acgcgactcc tgttccggga tgttttccaa cagcaccatc agcaactggc gttcgatctc
      421 ctgaatgaca tccatcatcc gcttaataac ctgcccggtg agatcctgaa aatcctgcgc
      481 catcatgatt tccagcagtt gcgcgttagt aaagctggta tgcgcgggta catctgccag
      541 aaattgtcgt gtatctgtta ccagttcacg ggcgtcggca aggtcaatcg gatcggcaaa
      601 ccagtcatcc caacgttggg ttaacgcttt tgctgatttc tccatttgat cctgatgcgg
      661 ttgtgacgcc tcaacactgt tcagcgcccg ctccgcagcc tgggcggtca tctgcacaac
      721 atagtacaaa cgatcgcgcg catcggggat ggcttccgcc gcttcggcaa tggcctgaac
      781 cagccccagt tcccgcaaac tgtcgcgcag catatgcgtc aggctgccga tgcgcgcaat
      841 gatatcgcca gctgaatgct cgtcagcagg tttgattgat ggttgcatca tagtcgcatc
      901 ctcacatgcc cagtttctca aagattttgt tgagtttttc ctccagcgtc gcggcggtaa
      961 atggcttcac cacatagcca ctggcccccg cttgcgccgc agcaatgatg ttctctttct
     1021 tcgcttctgc agtcaccatt aacactggca atgccgacat cgcgccatcc gcacgaattg
     1081 ttttcagcaa ttccaggcca tccatattgg gcatgttcca gtcggagata acaaatccat
     1141 aaccgcctgc ctgcaactta ttgagagcgt cgacaccatc ttccgcttcc tcaacattat
     1201 tgaatcccag ctctttcagc aggttacgca ctatgcgtcg catggtggaa aagtcatcca
     1261 caaccaaaaa tttaagttct ttatccgcca tttcacactc ctgatttaaa tacgtatcgc
     1321 ctgtccggca ctaattgttg ccaacatttg ctggcttacc tggctaagat cgaccacttc
     1381 gcagacacca cccatattga tggcctcgcg cggcatgccg aacaccacgc aacttgcttc
     1441 gttttgcgca agggtccatg cccccgcctg acgcatcgcc aacattcccg ccgcgccgtc
     1501 gttgcccata ccggtcagga tcaccccaac cgcattacgc cccgcctgtt tggcgacaga
     1561 atggaacaac acatctaccg aaggccgatg acggttaacc gccgggccgt cgtgaatttt
     1621 gatttgataa tttgcgccac tacgcgccag ttccatatgc cgatcgcccg gcgcaatata
     1681 ggcatgcccc ggcaagacac gttctccgtc ttcggcttct ttaaccccga tctggcaaag
     1741 cttattaagt ctgtcggcaa aagagcgggt gaaaccgggc ggcatatgtt gggtaattaa
     1801 caatgccggg ctggaaagcg gcaacggttg cagtacgtga cgaattgcct cagttccacc
     1861 cgttgaagca ccaatcgcaa tcagtttttc agaactcaac aacggccctg ccttcagcgt
     1921 tgtcggtgct gacaatggct tatgtgctgc aaggctcgcc tttgctgccg tacgcacctt
     1981 ttcagcaatc atttcgctat acgccagcat tccttcgcga atacccagtt gcggtttggt
     2041 gacaaaatct attgccccca gctccagcgc gcgcagcgtg acttctgatc ctttgccggt
     2101 caaggaagaa accatcacaa cgggcattgg acgcaaacgc attaattttt cgaggaaatc
     2161 cagtccgtcc atccgcggca tttcaacatc cagcgtcagc acatcgggat tgaatttctt
     2221 aatcaagtca cgcgcaacca gcggatcagg cgcggtcgcc accatttcca tgtcgctatg
     2281 gctgttgatg atttctgtca tgatctggcg catcagtgcc gaatcatcga cagataacac
     2341 cctgattttg ctcatcgtta atccttactt agcgcataca ccgtctgacc acgcagcgtg
     2401 aagcggcgct cgaggtggct aaagttttca gagtgacccg caaacagcaa tccgtcgggt
     2461 ttaaggagcg gaacaaagcg gcgcaaaatc tcctgctggg tagtttgatc gaagtagatc
     2521 atgacgttac gacagaagat cgcatcaaac ggccccggca cggtgtactg tttcgccagt
     2581 aaattcagcg gggcaaaatc aacatagttc gccagctcct gacgcacgcg taccagccct
     2641 tcatgcggtc ccgtccctcg catgaaatac cgttgcagtt gctgcggcgt caggtttttc
     2701 aactcttcat ggcgatagat accgcttctg gctttttcca gcacttcggt gtcgatatca
     2761 ctggcaaaca ctttccagcg tccgggcacg gtgcccaatg tgtcagccag agtcatcgca
     2821 atgctgtacg gctcttcgcc ggtcgaagcc gccgcgctcc atacgcgatc ctcgccagaa
     2881 cggcgacgtg cgtgatccgc gagcagaggg aaatgatgag cctcacggaa aaatgccgtc
     2941 agattcgtgg tcagcgaatt gataaacgcc tgccactcac cgctgtgctg attagattcc
     3001 agcaagttca gataatgacc gaaatccgtc agtcccagag aacgcaaacg acgaaccagt
     3061 cggttgtaaa ccatgtcgcg tttatggtca gccagaacga tcccggctcg ttgatagatc
     3121 aattgactta tccgccgaaa atgcgcgtcg gaaagcgcca ggcgctcggt catctgtaac
     3181 aataaagacg tttgcccaca gggcagagat gaagtcatag cgccttctca atcacttcag
     3241 gataccactg gcgcaatttg taactgcgcc gactcatgtc gtgctacttc atgttcttca
     3301 agggtaaata ccgccacacg cgacgaaaga tggtcggcct gattcgccag ttgttccgtt
     3361 gccaccgccg cttcttctac cagcgaggag ttctgctgag tcacctgatc catctggctg
     3421 acagcctgtg caacctgctc aatcccccgc cgttgttctt ccgacgccga agcaatttct
     3481 cccataatgt cgttcacgcg ggtcaccgaa ctgacaatat cggtcatggt cgcggcggcg
     3541 ttattcacca gtttcgaacc ctgctggaca cgattgactg actcttcgat cagccctttg
     3601 atctcttttg ctgcctgcgc gctacggctg gcaagattgc gcacttcacc tgccactacc
     3661 gcaaaaccac gcccctgctc tccggcgcga gccgcttcca ctgccgcatt cagggccaga
     3721 atattggtct ggaaagcaat tccgtcgata acgctgataa tgtcgccaat tttttgcgag
     3781 ctggtggcga tctcctgcat ggtgtgagtc atggtactga cctgaacacc gcccgcctgc
     3841 gccgttgtcg cggcattttt tgccagttcc gacgcctgtc gtgcgttatc ggcgttttgc
     3901 cctaccgtgg cggttaattg ctccatactg gcggccgttt gtgccagcga tgccgcctgc
     3961 tgttcggtac gacttgagag atcgttattg cctgcgacaa tctccgcgat cccaatgtgc
     4021 atctcatggc ttcccttacg cacatcactc accgtcccac gcaaagcctg ctgcatggtc
     4081 ttcaggctgg caaaaatggc ggtgatctca ttacgaccat ataccgcaat cggacgcgcc
     4141 agattacccg cagcaatgct gtcaaaatgg ctaccgataa tggccagtgg ttgaacaatc
     4201 atcttgcgcg tccaccacag cgcactgctg atgtagatcg ccgcaacaat aatcatgctg
     4261 ataaacacca gcgccgaaat ctgatagtta cgctggcttt gcgcactggc ggcttccagc
     4321 acatggttga tctccagctg ccaggcctca aagttaacgt caaacgcatt ctgtgatccc
     4381 tgcaccggcg cagtgaggaa atccgaaagt tgattacttt caagccaggt cgcctgatgt
     4441 tcgagatcgt tgtgccagcg ggcaaaactt ttctccgtct ctttttgcaa tgccctgacg
     4501 tgctcgttgc ccgcagtcat cgccataaaa cttttaaaca gcgtggtgga ttgcgtcaga
     4561 ctggcgcgcg ccgtcgtcat caacgtttta atgtcatccg ccggatagct aagcgctgtc
     4621 agagtgcctg ctttgttcag cgcggtactg gcctgtaaca ttaccgcccg agtttgcgct
     4681 aatgccgcac gttgctgatt gctctgctcg ccgtgattca gtcgttgcaa atcgtcgcga
     4741 aatgcccaaa aagacatgcc gttactgcca atctgcaaga tcccgcagag aatcaaaatt
     4801 aaaaacagcg tggtcgaaat tcgaatacga ttaaacatca acgctcccca tcaggcggca
     4861 atgaccgcgt tagtaaaaac tcgtcaaaat gtttcccagt ttggatcttg ttcagtaatt
     4921 cgcagtcgtg gctgtgccgg tggttgctca ctggcaggac gggatggtgt ttgcggttta
     4981 ttggtgagtg ggctggctgc cagacggaac gcggaaaccg cttgcgttaa acgactcgcc
     5041 tgttcttcca gcgcagctgc ggcggcagct gattcctgca ccagcgatgc gttctgttgc
     5101 gtgacgcgat ccatttccga aaccgccaat gcgacttgat cgatgccacg gctctgttca
     5161 tccgatgccg atgcaatctc gcccataatg tcagtcacgc gagtgacagc attgacgata
     5221 ttgttcattg tttccccggc gctttcgacc agcaccgaac cggtatcaat gcgcgagacg
     5281 gagtcttcaa tgagggcttt gatctctttt gccgccaggg cgctgcgact ggcaagatta
     5341 cgcacttcac ccgccaccac ggcaaaacca cggccctgtt cacccgcacg cgcggcttca
     5401 accgcggcat tcagcgcgag gatattagtc tggaaggcaa taccgtcgat aacgctgata
     5461 atgtcggcaa ttttcttcga actatcggcg atctcatgca tcgttttcac tacgccatcc
     5521 accactttgc cgccgtgctg ggcggtgtcg gaggcacttt gcgccagttg cgaggcctgg
     5581 cgggcgttat cggcgttttg cttcactgtc gcggtgagct gctccatgct ggcggcagtt
     5641 tcttccagcg cggatgcctg ctgttcagta cgggaggaaa gatcggtgtt gcccgccgca
     5701 atttcacggg taccggcata gatggcatct gaaccttcgc ggacatgagt gacggtgtca
     5761 gtcaaagagc gttgcatatg tgaaacgctc tgcgccaggt cacccatttc actgcgcccg
     5821 tcaatggtca gggtattcgc caggttacca ccggcgattt cgcaaatgtg agcaataatt
     5881 tttgccagcg gagtaagcaa catacggcga atgccgtacc acgccaccag cagaatcaat
     5941 accaccacca gcgcgataac cgccagttgc cactgggcaa atcggtaatc atctgcgttg
     6001 tcagtgacga tatcgcgata cagtttttca ctgctgaggg cgtactgagc aaacgcttcg
     6061 cccattgcat tttgcattcc ctgggttggc tgagcgaaat aagctccagt attgccataa
     6121 tcaagataat caatcagttc agttaacgct gtgtaatagt ttttatattt ttcatcaaaa
     6181 ttacggctgg tagcgaccat ttcaggtaac ggtgccatgc ttttgaattt tttataatgc
     6241 gtcgcggcct gggccaatgt tttcctggcg ctatcgagca attcaacttt ggcgttactt
     6301 tgttgattgg aggaatccat catcatccgc accgctgaac gactcaggtt aatgcgcgtt
     6361 tgcagcatta aatcccaggt tgacgtcagc tcgccctgct gttcccgtaa ttgattggaa
     6421 accacaaagc tcttctggct atggtgaagg gaagaaaaaa acaaactgcc ggaaataagc
     6481 tgtaacagtg cgaatacccc cagcaccatt accaacagcg tgactacgcg gatacggtta
     6541 atcataaggc accttcctga aaacaagttg atctcgttat cggcaagaag gggggaaact
     6601 ttattgctga tgccacccgc cgcgaaattg aaataaaaaa cccgatgcgc agatcatcgg
     6661 gttcatttca attgaggaaa tcgggagaat tacgccactt ctgacgccgc gctatctaac
     6721 agcgccatct cttcgctgtt cagcagtttt tcgatgttca ccagaatcaa catacggtcg
     6781 cccagtgcgc ccagtccagt gagatattct gttgaaagcg tcacggcaaa ctctggtgcc
     6841 ggacgaattt gctccgccgt caatgaaagc acgtctgaaa cgccgtcaac cacgatgccg
     6901 accacccgct gtccgagatt caggacgata actaccgtgt tgtcgttata gtccacatcc
     6961 acctggctga acttaattcg taagtcaaca atcggcacaa taacgccgcg cagattcgtg
     7021 acgcctttga taaacgctgg cgtgttcgca atccgtgtta cctgatcgta gccacggatc
     7081 tcctgcactt tcaggatatc aataccgtac tcttcatcac caagggtaaa taccagaaat
     7141 tcctggcctg acggctcgct ggccagcttt gttacattcg tcataccggt catattgtta
     7201 cctttttact cattcaggcg gcggtgttcg ccatacgttg ttcgcggttt atcgcctgca
     7261 aggcggagac atcaacaatc agtgccacgc tgccgtcgcc aagaatggtc gcagcagaaa
     7321 tgccggggac tttgcgatag ttactttcaa ggtttttaac cacaacctgg tgttgaccaa
     7381 ttaattgatc caccagcaag gcgtagcggc gaccgccact ttgtaagatc accacaattc
     7441 cctgggtggc ttcggttttc gcgcccgcga cgttgaacac tttccacagt tcgacgatgg
     7501 gcagatattc accccgcact tccagcaccc gctcgccgcc ggccagtgga tggagatcgg
     7561 cttcacgggg ttgcagtgat tccataacag cattcagcgg cagaatgaaa acttcatccg
     7621 caacgcgtac ggacatgccg tcgaggatgg ccagcgtcag cggcagtaaa atgcggatcg
     7681 tagtgccagt accctgcttc gactggattt cgacatgacc gcccatctcc tggatattac
     7741 gtttaacgac gtccatgccg acgccgcgcc cggagacgtc ggtgacctgc tctgccgtgg
     7801 agaagcctgg tgcaaatatc agcattgcaa cttcgtcgtc gctcatgttt tcgctgacag
     7861 tcaaaccttg cgaggccgct tttgccagaa ttcgctcacg gtttagcccc gccccatcgt
     7921 cggtcacttc aatgcaaatg ttgccgccct gatgttcggc agacagaatt aaatttccga
     7981 cgctgttttt acctgcggcg agccgttttt ctggcagttc aataccgtga tcgaggctat
     8041 tgcgtaccag gtgggtcagc gggtcgataa tgcgttctat caggctcttg tcgagctcgg
     8101 tggaactgcc caccagcgtc agttctacct gcttgccgag ttttcccgcc agatcacgca
     8161 ccagccgggg atagcgacta aagacatatt ccatcggcat catgcgaatc gacatcactg
     8221 attcctgcaa atcacgggcg ttacgttgta actgccccat gctggtaatc aaatcaccat
     8281 gattaaccgg gtccagttcg ctggaacgct gggcaagcat ggactgggtg ataaccagct
     8341 cgccgacgag gttaattaat tgatcaacct tttctaccgc tacacggatg ctggtggatt
     8401 cactgctgcg cgtcgttttt tcccgctcca cgcggccggt tggcgcttgt tcggctgcca
     8461 gtttaagcac aggtggggta gctatttttg gcgaaacttc tactgtttca aaggtaatct
     8521 gatcggcttc aatcacaaaa cagagtaccg ctgtgatgtc atcttcggcg atatcgcccg
     8581 gtaatattgc cgagagagaa tccgcccctt tcaccacgtc atttaacgtt gtcagatgcc
     8641 ccagctcttc ttccagcagg tcgacttcgc cggccttcag gcgcgaaagg ataattcgtc
     8701 gcggcaactg actgcgactt tgctcatctt gcggttcact tttggcaacc acacttaatc
     8761 gggtcactgc ggatggcgtt tcgcctttcg cttctaatgc cagttgacgc aaggcctggc
     8821 agatataatc gtagctggcg gcatccggct cttgcgactg tttataagcg tcgagctgtt
     8881 cttgcatgat gtccttcgtt tccaaaaaca gattgataat gtcggtgttg agttgcatct
     8941 cacctcgtct ggcttcatcg agcaggtttt ccatcagatg cgtggtttcc tgcaaaacgc
     9001 tgaagccaaa agttcctgcc cctcctttga tcgagtgggc agcccgaaag atggcattca
     9061 attgttcggc atctggcgct tccggctgca aaaccagcag atgctgctcc atatcagcca
     9121 acagttcgtc cgcttcatca aaaaatgtct gataaaaatc gcttatatcg atgctcacgc
     9181 tgtcacctcg gttcggctga tggcattgtg ggaacactga cctgtggtgc aacctcaggt
     9241 ttttctaggg cgcttactgg ctcattctgg ctttcggcgt tttcatgcaa aatggcctgt
     9301 tcggcttgtt tgttcagtac caacaggctg atgcgacggt tgacggcatc atcaggtccg
     9361 cgatcgctta agcgcatcgt tgccgccatg ccgacgacac gtaacacttt gccgctatcc
     9421 aaccctccga ccatcagttc gcggcgggat gcattggccc gatcggcaga aagctcccag
     9481 ttgctgtaac ctttctcacc gctggcgtag ggaaaatcat cggtatgacc tgaaaggcta
     9541 atacggttgg gaataccgtt cagcacaggc gcgatggcgc gcagaatgtc gcgcatatag
     9601 ggttcgacat cggcactgcc ggttctaaac atcgggcgat tctggctatc gatgatctga
     9661 atacgtagac cttcctggac cagatcgatt ttgagatgga gacgtaacgc ccgcagtttc
     9721 ggatcggact ctatcaactg gtcgagatca ccccgcaatt tccgcaatcg actttgctcc
     9781 atgcgttttt tcagctcttc gatgttcggc tgcttattca cttccccctg gctttgggtg
     9841 taatcatcac caccgccggg aattgggctt tcactattgg aaatgcgatc gccgcccgta
     9901 accgctgtcg ccagtggagt ccggaagtac tccgcaatct gaatcaactc ttttgggctg
     9961 gagatggaga tcagccacat caccagaaaa aaggccatca tcgcagtcat aaagtcggca
    10021 taagcaatct tccacgatcc atgtgctgcc ccgtggcttt tggctttgcg tcgtttgacg
    10081 acaataatcg gatgcgcttg attcttcatg cttcctcggt tgtcgtctgt tgttgcggat
    10141 ttttcaccgc acgcacatgc tcttccagtt caataaacga cggacgttcg ctggaataga
    10201 gcgttttgcg accaaactca acggcgatag gcggtgcgta accgttcaga ttagaaagca
    10261 gagtgacttt gacgcactgc atcattttgc tggtttcggc gcttttctga cgtaaaacac
    10321 tcgctagtgg ggaaataaat ccgtaagcca ataaaatacc gaggaaagtc cccaccatcg
    10381 catgtgcgat aagcgccccc agctcggcgg caggacgatc ggctgaacct aacgcgtgaa
    10441 cgccccccat tacagccgcg acaataccaa acgccggaag tgagtccccg accagcgcca
    10501 gactgttcgc cgggacttct gcctcgcttt cgtgcgtctc aatctcttca tccatcaggg
    10561 cttcgatttc gaaggtgttc atgtgaccgc tgataatcag gcgcagataa tcgacgataa
    10621 aatcaagcat gacgctatcc gcgaggatgc gtgggtagct ggcgaagatc tcgctctcac
    10681 ggggattttc aatatcacgt tccagcgaaa acatccccat ctgccgcgat ttcgccatca
    10741 accgataaag cagagccagc agatccatat acattgcttt ggtgtatttg gagcgacgaa
    10801 acagcaacgg cagcgctttc agcgtgcctt taatcgcttt gccattattg ccgacgataa
    10861 acgacccaat ccctgcaccg gcaataatca ccagttcagc gggttgatag agtgctccaa
    10921 ggcttccacc ggtcatcaaa taaccgccga aaactgtacc gagaacaacc aggtaaccta
    10981 ataagataag cacgacatca tccttccact gttgaccatg acaggatgtt cagtcgtcag
    11041 gcgttaacgc gcgattgggg aaaaaaaaag cagcggtacg tcgttaccgc tgctggaatg
    11101 ttgcgccaca ccgtatcagt taaacagcct gtactctctg ttcatccagc agttgtggga
    11161 taatatcggc aggattctgg gaaagtttac gtctttttac tgcccgggat ggcggttgac
    11221 ataagctgca ggcaaagctg ccaacaggct ggtgagcgtg ggtaataaaa ttgccgccgc
    11281 agcagttgca gctggaaagt tgcagtaatc cactttcaac aaaccgcacc aatgtccagg
    11341 cacgggttaa tgccagcagt ggtccttctt ctgcttgtgg gcactgttca aggtataaac
    11401 ggtaggcttt gatcaccgca tcgacgccat tacacaaacc ggttttcagt aaaaactgcc
    11461 atgcattaca gaacatcgaa gcatgaacgt tttgttccca ggtcataaac cagtcggttg
    11521 agaatggcag catgcctttc ggcggtgggc ttccgcgcag ttctttataa agttttatca
    11581 ggcgtccgcg acttaactgt gtttcgcttt ccagcatctg caaacgagcg cccagggtga
    11641 tcaattccat tgccagctga atatcccgcg cttcctgaac aatgcttttt tcactcatga
    11701 tcaggccctt ttcttgcgca gcgcttcttc aggctgatta acatcattca gcaagcgtgt
    11761 tgagagcatg atgccggtat gaatttgctg gagatcgtca acgcgggaat cttgcgtcaa
    11821 ctgagtaatc gtctggtggc tgtcaaaacg gaagtgacaa accagctgat tggtttctgc
    11881 cagcttaacc atttgcggaa ga
//
LOCUS       DAJTRN010000111        11721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRN010000111 DAJTRN010000000
VERSION     DAJTRN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            27..896
                     /locus_tag="OO328_003299"
     CDS             27..896
                     /locus_tag="OO328_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5931750.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1099..1452)
                     /locus_tag="OO328_003300"
     CDS             complement(1099..1452)
                     /locus_tag="OO328_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5931751.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1590..3236)
                     /gene="groL"
                     /locus_tag="OO328_003301"
     CDS             complement(1590..3236)
                     /gene="groL"
                     /locus_tag="OO328_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5931752.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3280..3573)
                     /locus_tag="OO328_003302"
     CDS             complement(3280..3573)
                     /locus_tag="OO328_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5931753.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3849..5105
                     /gene="yjeH"
                     /locus_tag="OO328_003303"
     CDS             3849..5105
                     /gene="yjeH"
                     /locus_tag="OO328_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5931754.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5121..5597)
                     /gene="fxsA"
                     /locus_tag="OO328_003304"
     CDS             complement(5121..5597)
                     /gene="fxsA"
                     /locus_tag="OO328_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5931755.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5934..7370
                     /gene="aspA"
                     /locus_tag="OO328_003305"
     CDS             5934..7370
                     /gene="aspA"
                     /locus_tag="OO328_003305"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5931756.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7488..8789
                     /gene="dcuA"
                     /locus_tag="OO328_003306"
     CDS             7488..8789
                     /gene="dcuA"
                     /locus_tag="OO328_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5931757.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8905..9243
                     /gene="cutA"
                     /locus_tag="OO328_003307"
     CDS             8905..9243
                     /gene="cutA"
                     /locus_tag="OO328_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5931758.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9219..10916
                     /gene="dsbD"
                     /locus_tag="OO328_003308"
     CDS             9219..10916
                     /gene="dsbD"
                     /locus_tag="OO328_003308"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5931759.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10941..11516
                     /locus_tag="OO328_003309"
     CDS             10941..11516
                     /locus_tag="OO328_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5931760.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11623..11698
                     /locus_tag="OO328_003310"
     tRNA            11623..11698
                     /locus_tag="OO328_003310"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11656..11658,aa:Phe,seq:gaa)
BASE COUNT         2800 a         3044 c         2896 g         2981 t
ORIGIN      
        1 ttgagaaatg gttagggaga acctacatgg ccataagcat caagggtgtt aataccggtg
       61 ttattcgcaa gagtaataat ttcattgcac tggcgctgaa aattaaagaa cctcgcaata
      121 aagaatcgtt gttctttatg tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa
      181 gtcgtctgca tcaaaagcat aaactggatg ctgctgcacg ccttcagtat gagcaagctc
      241 gcgataaagt cattaagaaa atggcagaaa acatcccaga aattctggtt gatgagctta
      301 aaaatgccga tattaatcgt cgggttaaca cgctggaact taccgataat caaggcgaaa
      361 atcttacctt cgtattaacg ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt
      421 tgcagattga aatgctggcg cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg
      481 aactggcatt acgtattacc tcactgttag atttcctgcc gttatacgac gttgactgcc
      541 aggacaatgg caatctggaa tatgacacct attcccaacc ggaatggaaa cataacttgt
      601 tcgaccatta tctggcggtg ctttaccgct ttaaagatga aagtggcaaa gaacaattta
      661 gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc
      721 gccgaatgct tgatttcagc ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct
      781 atgttcgcac cgtagcggcg aataacgccc aaccgctaac ccaggatcaa tgcttacgcg
      841 cgctgcatca cttacgtgta caatccacca gcaaaactgc accacaggcg aaataatctc
      901 atctttgcta atgcgaggct ggagccatcc agcctgaaat gactgataac gtgatcgttg
      961 gtcatgccgg atgcggcgta aacgccttat ccggcctaca aattctcaag cattcaatca
     1021 gttgcaatgc ctgctgtagg cctgataagc gaagcgcatc aggcaataat acgtttgtca
     1081 cccagcctct ggatcacatc agttcgggca cttataaacc tgaccgataa tctggctatc
     1141 cgtcgggaca aaactggaca acattccctg cgacgggctg ctgatgccat aaatcacgtt
     1201 accgcccatt gcagccgcct ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc
     1261 ctcttctccg tgttgcccgg aaagccagtt gctttgctta cctgtcgcag taccaatcag
     1321 ctggcactct gcgcctggct gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa
     1381 ttcgttgctg gagctacaac ccgccatcag tagcgcggca ccgacaatcc ctgctaagta
     1441 ttttacgtgc atgttattcc ccataatcaa tgagctggac gccactcgcg tcatccgtgt
     1501 ctgaatctta tactagaaag atgaccaaaa gaaaaacccc cagacatttc tgcccggggg
     1561 tttgtttatt tctgcgaagt gcagggcaat tacatcatgc cgcccatgcc acccatgccg
     1621 cccataccgc cagcagcgcc taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg
     1681 cattcggtgg tgatcatcag gccagccaca gaagctgcgt actgcagagc agaacgggtt
     1741 actttggttg ggtccaggat acccatgtcg atcatgttgc cgtattcttc ggttgctgcg
     1801 ttgtaaccgt agttgccgtc gccgccttta acggtgttag caacaacaga cggttcttcg
     1861 ccgcagttca ggacgatctg acgcagcgga gcttccattg cacgcagtgc aactttgata
     1921 cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca gtttagacgc tacgcggatc
     1981 agcgcaacac caccaccagc aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg
     2041 gcatcttcaa cgcgtgcttt tttctctttc atttcaactt cggtagcagc acctactttg
     2101 ataactgcaa cgccgcctgc cagtttcgct acgcgctcct gcagtttttc acggtcgtag
     2161 tcagaagttg cttcttcaat ctgctgacgg atctgagcaa cacggccctg gattgcagct
     2221 tcttcaccca cgccatcgat gatagtggtg gtgtctttgt tgatcacaac acgtttagcc
     2281 tgacccaggt cttccagggt tgctttttcc agctccatac cgatctcttc agagattacg
     2341 gtaccgccag tcagggttgc gatatcctgc agcatagctt tacgacgatc gccgaagccc
     2401 ggagctttaa ccgcagcaac tttcacgatg ccacgcatgg tgttaacaac cagagttgct
     2461 agcgcttcgc cttcaacatc ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca
     2521 gcttccagaa ccggcagcat ttcgcggatg ttggagattt tcttgtcagc cagcaggatg
     2581 aacgggcttt ccagttctac tgcgccagtt tccggcttgt tgatgaagta aggagacagg
     2641 tagccacggt cgaactgcat accttcaacc acgtccagtt cgtcctgcag accggtaccg
     2701 tcttcaacgg tgataacgcc ttctttaccg actttgtcca tcgcttcagc gatcagttta
     2761 cctacggttt cgtcggagtt agcggagatg gtaccaacct gagcaatcgc tttagaatca
     2821 gagcacggta cggacagcgc tttcagttct tcaactgcag cggtaaccgc tttgtcgata
     2881 ccacgtttca ggtccatcgg gttcatgccc gcagcaacag ctttcagacc ttcagtgatg
     2941 atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg
     3001 gaggcaactt ctttcaccat ctgcgcaccc atattttcga acttgtcttc cagttcgatt
     3061 tcacgagcaa cggaaacacc atctttggtg atggtcggtg caccgaaaga tttatccaga
     3121 actacgttac ggccttttgg accgagggta actttcactg catctgccag tacgtttacg
     3181 ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta cgtctttagc tgccattatc
     3241 tttattcctt aaattcgtat gttcagtgtc gtgcgcggat tacgcttcaa caattgccag
     3301 aatgtcgctt tcggacatga tcaacacttc ttcattgtcg atcttctcgg atttcacacc
     3361 gtagccatcg ttgaaaataa cgatgtcgcc aactttcacg tccagcggct tcacttcgcc
     3421 attttcaagg atacggccat tgccgacagc cagcacttcg ccgcgggtgg atttagccgc
     3481 tgcagagccg gtcagaacga tgccgccagc agatttagtt tcaacttctt tacgcttgac
     3541 gatcacgcga tcatgcaatg gacgaatatt cattgataac tctcctttga gaaagtccgt
     3601 atctgttatg ggtgacgccg gagcttacgt ggtttcccgg ctggtgacca gagaaatggg
     3661 gatgaggctt cgccccttca agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg
     3721 ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc ttcatcgccg tcataagcga
     3781 atctgattgt gctaccatcg aaaatctacg cagttgccca aaatttgggc gcaatcggat
     3841 atcaacccat gagtggactc aaacaagaac tggggctggc ccagggcatt ggcctgctat
     3901 cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag
     3961 gcaataacag cctgtgggcg tggcccgttt tgattatctt agtgttcccg attgcgattg
     4021 tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta
     4081 tggcgtttgg ttcgcggctt gagcgagtca ccggctggct gtttttatcg gtcattcccg
     4141 tgggtttgcc tgccgcacta caaattgccg ccggattcgg ccaagcaatg tttggctggc
     4201 atagctggca actgttgttg gcagaactcg gtacgctggc gttggtgtgg tatatcggta
     4261 ctctcggtgc cagttccagt gctaatctac aaaccgttat tgccggactt atcgtcgcac
     4321 tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc
     4381 caggaaatat cgaacttacc gggttatttg ctgcgttatc agtgatgttc tggtgttttg
     4441 tcggtctgga agcatttgcc catcttgcct cggaatttaa aaatccagag cgtgattttc
     4501 ctcgtgcttt gatgattggt ctgctgctgg caggattagt ctactggggc tgtacggtag
     4561 tcgtcttaca cttcgacgcc tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa
     4621 ttgtagtaca gttgttcggt gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg
     4681 cctgctttgc cagtcttaac atttatatac agagcttcgc ccgcctggtc tggtcgcagg
     4741 cgcaacataa tcctgaccac tacctggcac gcctctcttc tcgccatatc ccgaataatg
     4801 ccctcaatgc ggtgcttggc tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga
     4861 tcaatctgga cgctcttatt atttatgcca atggcatctt tattatgatt tatctgttat
     4921 gcatgctggc aggctgtaaa ttattgcaag gtcgttatcg actactggcg gtggttggcg
     4981 ggctgttatg cgttctgtta ctggcaatgg tcggctggaa aagtctctat gcgctgatca
     5041 tgctggtggg gttatggctg tttctgccaa aacgaaaaac gccggaaaat ggcataacca
     5101 cataatccgg cgtttcgaca ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg
     5161 tcatcatcct ttcgctgata ctcaccatca aaagtattac cgccaccggt cccggcgcta
     5221 aaaccgccgc ctggcatgcg agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc
     5281 ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat
     5341 aaaagcagca aaccggcaat aatcagcgaa acacttttaa tcatctccgc cgcagggttt
     5401 tcacccgccg ccattttttg ctgcatcagc acgaaattct taaagccctg gttacgtacc
     5461 agtgacatac cgataactga cgtgaatata accagcacga gggtcagcaa tacccccaat
     5521 acatgggcaa cctgaataaa gattgaaatc tcaatataga cataaaggaa aatggcaata
     5581 aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc ggttaaaccc ggctacctga
     5641 atgggttgcg aatcgccttc agcttatatt gtggtcatta gcaaaatttc aagatgtttg
     5701 cgcaactatt tttggtagta atcccaaagc ggtgatctat ttcacaaatt aataattaag
     5761 gggtaaaaac cgacacttaa agtgatccag attacggtag aaatcctcaa gcagcatatg
     5821 atctcgggta ttcggtcgat gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca
     5881 tatattctgt gtgtttaaag caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa
     5941 acaacattcg tatcgaagaa gatctgttgg gtaccaggga agttccagct gatgcctact
     6001 atggtgttca cactctgaga gcgattgaaa acttctatat cagcaacaac aaaatcagtg
     6061 atattcctga atttgttcgc ggtatggtaa tggttaaaaa agccgcagct atggcaaaca
     6121 aagagctgca aaccattcct aaaagtgtag cgaatgccat cattgccgca tgtgatgaag
     6181 tcctgaacaa cggaaaatgc atggatcagt tcccggtaga cgtctaccag ggcggcgcag
     6241 gtacttccgt aaacatgaac accaacgaag tgctggccaa tatcggtctg gaactgatgg
     6301 gtcaccagaa aggtgaatat cagtacctga acccgaacga ccatgttaac aaatgtcagt
     6361 ccactaacga cgcctacccg accggtttcc gtatcgcagt ttactcttcc ctgattaagc
     6421 tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg taaagctgtc gaattccagg
     6481 acatcctgaa aatgggtcgt acccagctgc aggacgcagt accgatgacc ctcggtcagg
     6541 aattccgcgc tttcagcatc ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg
     6601 aactgctgct ggaagttaac cttggcgcaa cagcaatcgg tactggtctg aacacgccga
     6661 aagagtactc tccgctggca gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac
     6721 cggctgaaga cctgatcgaa gcgacctctg actgcggcgc ttatgttatg gttcacggcg
     6781 cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt
     6841 ctggtccacg tgccggcctg aacgagatca acctgccgga actgcaggcg ggctcttcca
     6901 tcatgccagc taaagtaaac ccggttgttc cggaagtggt taaccaggta tgcttcaaag
     6961 tcatcggtaa cgacaccact gttaccatgg cagcagaagc aggtcagctg cagttgaacg
     7021 ttatggaacc ggtcattggc caggccatgt tcgaatccgt tcacattctg accaacgctt
     7081 gctacaacct gctggaaaaa tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag
     7141 gttacgttta caactctatc ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca
     7201 acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc
     7261 tggaacgcgg tctgttgact gaagcggaac ttgacgatat tttctccgta cagaatctga
     7321 tgcacccggc ttacaaagca aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg
     7381 tagtacaaat aaaaaaggca cgtcagatga cgtgcctttt ttcttgtgag cagtaactta
     7441 aaaataacaa tctaatatca acttgttaaa aaacaaggaa ggctaatatg ctagttgtag
     7501 agctcatcat agttttgctg gcgatcttct tgggcgccag attgggggga ataggtattg
     7561 gttttgcagg cggattgggg gtgctggttc ttgccgctat tggcgttaaa cccggtaaca
     7621 tcccgttcga tgtcatttcc attatcatgg cggttatcgc cgctatttct gccatgcagg
     7681 ttgctggcgg tctggactat ctggttcatc agacagaaaa gctgctgcgc cgtaacccga
     7741 aatacatcac gatcctcgca ccgatcgtga cctatttcct gactatcttt gctggtactg
     7801 gcaacatctc tctggcgaca ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta
     7861 aaccttgccg tccgctctct actgcagtgg tatccgcgca gattgcgatc accgcatcgc
     7921 caatctcagc ggcagtggtt tacatgtctt ccgtgatgga aggtcatggc atcagctacc
     7981 tccatctgct ctccgtggtc atcccgtcca ccctgctggc ggttctggtg atgtccttcc
     8041 tggtcactat gctgttcaac tccaaactct ctgacgatcc gatttatcgc aagcgtctgg
     8101 aagagggctt ggttgaactg cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa
     8161 cgtccgtctg gctgttcctg ctgggcgtag ttggcgtggt tatctatgca atcatcaaca
     8221 gcccaagcat gggtctggtt gaaaaaccgc tgatgaacac caccaacgca atcctgatca
     8281 tcatgctcag cgttgcaact ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc
     8341 tcaactccag caccttcaaa gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt
     8401 ggctgggcga tactttcgtt tccaacaaca tcgactggat caaagatacc gctggtgaag
     8461 tgattcaggg tcatccgtgg ctgctggccg tcatcttctt ctttgcttct gctctgctgt
     8521 actctcaggc tgcaaccgca aaagcactga tgccgatggc tctggcactg aacgtttcac
     8581 cgctgaccgc tgttgcttct ttcgctgcgg tgtctggtct gttcattctg ccgacctacc
     8641 cgacgctggt tgctgcggta cagatggatg acacgggtac tacccgtatc ggtaaattcg
     8701 tcttcaacca tccgttcttc atcccgggta ctctgggtgt tgccctggcc gtttgcttcg
     8761 gcttcgtgct gggtagcttc atgctgtaat gatcaatcgc ggggcgttca cgccccgctt
     8821 tctttcccgc cgactaacat ccttcccccg tccgttgtat agtgacctct ctcttgcggt
     8881 tccatctgtt cttgcgaggt gtttatgctt gatgaaaaaa gttcgaatac cacgtctgtc
     8941 gtggtgctat gtaccgcacc ggatgaagcg acagcccagg atttagccgc caaagtgctg
     9001 gcggaaaaac tggcggcctg cgcgaccttg atccccggcg ctacctctct ctattactgg
     9061 gaaggtaagc tggagcaaga atacgaagtg cagatgattt taaaaactac cgtatctcac
     9121 cagcaggcac tgctggaatg cctgaagtct catcatccat atcaaacccc ggaacttctg
     9181 gttttacctg ttacacacgg agacacagat tacctctcat ggctcaacgc atctttacgc
     9241 tgattctgct actttgcagc acttccgttt ttgccggatt attcgacgcg ccgggacgtt
     9301 cacaatttgt ccccgcggat caagcctttg cttttgattt tcagcaaaac caacatgacc
     9361 ttaatctgac ctggcagatc aaagacggtt actacctcta ccgtaaacag atccgcatta
     9421 cgccggaaca cgcgaaaatt gccgacgagc agctgccgca aggcgtctgg catgaagatg
     9481 agttttacgg caaaagcgag atttaccgcg atcggctgac gcttcccgta accatcaacc
     9541 aggcgagtgc gggagcgacg ttaactgtca cctaccaggg ctgtgctgat gccggtttct
     9601 gttatccgcc agaaaccaaa accgttccgt taagcgaagt ggtcgccaac aacgcagcgt
     9661 cacagcctgt gtctgtttcg cagcaagagc agcacaccgc gcaattgccc ttttccgcgc
     9721 tctgggcgtt gttgatcggt attggtatcg cctttacgcc atgcgtgctg ccaatgtacc
     9781 cactgatttc tggcatcgtg ctgggtggta aacagcggct ctccactgcc agagcattgt
     9841 tgctgacctt tatttatgtg caggggatgg cactgactta cacggcgctg ggtctggtag
     9901 ttgccgccgc agggttacag ttccaggcgg cgctacagca cccatacgtg ctcattggcc
     9961 tcgccatcgt ctttaccttg ctggcgatgt caatgtttgg cttgtttact ctacaactcc
    10021 cctcttcgct gcaaacacgt ctcacgttga tgagcaatcg ccaacagggc ggctcaccta
    10081 gcggtgtgtt tgttatgggg gcgattgccg gactgatctg ttcaccatgc accaccgcac
    10141 cgcttagcgc gattctgctg tatatcgccc aaagcgggaa catgtggccg ggcggcggca
    10201 cgctttatct ctatgcgttg ggcatgggcc tgccgctgat gctaattacc gtctttggta
    10261 accgcttgct gccgaaaagc ggcccgtgga tggaacaagt caaaaccgcg tttggttttg
    10321 tgatcctcgc actgccggtc ttcctgctgg agcgagtgat tggtgatgta tggggattac
    10381 gcttgtggtc ggcgctgggt gtcgcattct ttggctgggc ctttatcacc agcctacagg
    10441 ctaaacgcgg ctggatgcgc gtggtgcaaa taatcctgct ggcagcggca ttggttagcg
    10501 tgcgcccact tcaggattgg gcatttggtg cgacgcatac cgcgcaaact caaacgcatc
    10561 tcaactttac acaaatcaaa acggtagatg agttaaatca ggcgctcgtt gaagccaaag
    10621 gcaaaccggt gatgttagat ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga
    10681 aatacacctt cagcgacccg caggtgcaaa aagcgttagc agacacggtc ttacttcagg
    10741 ccaacgtcac ggccaacgac gcacaagatg tggcgctgtt aaagcatctt aatgtccttg
    10801 gcctaccgac aattctcttt tttgacggac aaggccagga gcatccacaa gcacgcgtca
    10861 cgggctttat ggatgctgaa accttcagcg cacatttgcg cgatcgccaa ccgtgaacga
    10921 cacttgcagt gggaaatagc gtgcaacgtg aagatgtact gggagaagcc ctgaaattat
    10981 tagaattaca agggattgcc aacaccacgc tggagatggt tgctgaacgt gtggattatc
    11041 cactggacga gctacgccgc ttctggccag acaaagaggc aatcctctac gatgcgctgc
    11101 gttatcttag ccaacagata gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa
    11161 ccgccgaaca aaagctgctg gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc
    11221 gctatccggg ctgtctattt atcgctgcct gtacgtttta tcccgatcct ggccacccta
    11281 ttcatcaact ggccgatcag caaaaaagcg ctgcctacga tttcacccac gaactgttaa
    11341 ccacgctgga agttgacgat ccggcgatgg tagcaaagca gatggaactg gtgctggaag
    11401 gctgtttaag ccgaatgctg gtgaatcgta gccaggcgga tgtcgacact gcacatcggc
    11461 tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg tggtgcattg acctgacaga
    11521 aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt
    11581 aggttgacga gatgtgcaga ttacggttta atgcgccccg ttgcccggat agctcagtcg
    11641 gtagagcagg ggattgaaaa tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa
    11701 ttcatatcaa cggacctcca c
//
LOCUS       DAJTRN010000112        11578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRN010000112 DAJTRN010000000
VERSION     DAJTRN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..346
                     /locus_tag="OO328_003311"
     CDS             <1..346
                     /locus_tag="OO328_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5931761.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO328_003312"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO328_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5931762.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO328_003313"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO328_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5931763.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO328_003314"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO328_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5931764.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO328_003315"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO328_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5931765.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO328_003316"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO328_003316"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5931766.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO328_003317"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO328_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5931767.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO328_003318"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO328_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5931768.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO328_003319"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO328_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5931769.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO328_003320"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO328_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5931770.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO328_003321"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO328_003321"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5931771.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO328_003322"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO328_003322"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5931772.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO328_003323"
     CDS             complement(8961..9236)
                     /locus_tag="OO328_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5931773.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO328_003324"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO328_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5931774.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO328_003325"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO328_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5931775.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO328_003326"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO328_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5931776.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO328_003327"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO328_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5931777.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..>11578)
                     /locus_tag="OO328_003328"
     CDS             complement(11073..>11578)
                     /locus_tag="OO328_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5931778.1"
                     /translation="HAQKYNKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPG
                     YSTPDYTGQTIGYVVTLPAQMPNCCSSELPTIDLYIDQLHTVTGVSNALGFIIAALLN
                     AYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENG
                     EWIYLRN"
BASE COUNT         2735 a         2849 c         3070 g         2924 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 tgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaa
//
LOCUS       DAJTRN010000113        11358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRN010000113 DAJTRN010000000
VERSION     DAJTRN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO328_003329"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO328_003329"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO328_003330"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO328_003330"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5931779.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO328_003331"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO328_003331"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5931780.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO328_003332"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO328_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO328_003333"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO328_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5931781.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO328_003334"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO328_003334"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5931782.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO328_003335"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO328_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5931783.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO328_003336"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO328_003336"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5931784.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO328_003337"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO328_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5931785.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO328_003338"
     CDS             9553..10332
                     /locus_tag="OO328_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5931786.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO328_003339"
     CDS             10322..11131
                     /locus_tag="OO328_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5931787.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..>11358
                     /locus_tag="OO328_003340"
     CDS             11218..>11358
                     /locus_tag="OO328_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IID with IIC forms the translocation channel;
                     involved in N-acetylgalactosamine transport; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIA"
                     /protein_id="HCR5931788.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQ"
BASE COUNT         2722 a         3076 c         2850 g         2710 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcag
//
LOCUS       DAJTRN010000114        11262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRN010000114 DAJTRN010000000
VERSION     DAJTRN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            308..1948
                     /gene="trg"
                     /locus_tag="OO328_003341"
     CDS             308..1948
                     /gene="trg"
                     /locus_tag="OO328_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5931789.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1986..2909)
                     /locus_tag="OO328_003342"
     CDS             complement(1986..2909)
                     /locus_tag="OO328_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5931790.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3126..4469
                     /locus_tag="OO328_003343"
     CDS             3126..4469
                     /locus_tag="OO328_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5931791.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4694..6349
                     /gene="opgD"
                     /locus_tag="OO328_003344"
                     /gene_synonym="mdoD"
     CDS             4694..6349
                     /gene="opgD"
                     /locus_tag="OO328_003344"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5931792.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6489..6713
                     /locus_tag="OO328_003345"
     CDS             6489..6713
                     /locus_tag="OO328_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5931793.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6776..7312
                     /gene="rimL"
                     /locus_tag="OO328_003346"
     CDS             6776..7312
                     /gene="rimL"
                     /locus_tag="OO328_003346"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5931794.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7307..8287)
                     /locus_tag="OO328_003347"
     CDS             complement(7307..8287)
                     /locus_tag="OO328_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931795.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8411..9403
                     /gene="tehA"
                     /locus_tag="OO328_003348"
     CDS             8411..9403
                     /gene="tehA"
                     /locus_tag="OO328_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5931796.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9400..9993
                     /gene="tehB"
                     /locus_tag="OO328_003349"
     CDS             9400..9993
                     /gene="tehB"
                     /locus_tag="OO328_003349"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5931797.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10296..10952
                     /locus_tag="OO328_003350"
     CDS             10296..10952
                     /locus_tag="OO328_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5931798.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10970..11248)
                     /gene="tnpA"
                     /locus_tag="OO328_003351"
                     /pseudo
     CDS             complement(10970..11248)
                     /gene="tnpA"
                     /locus_tag="OO328_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2904 a         2637 c         2832 g         2889 t
ORIGIN      
        1 ggccttcgtc tttctgattc ccggttagcc tgaaaacaga aagtctcagg cacccgcagg
       61 catcctatga ggtttcctta gggacgaaaa taatcacttc acgaaattgc gtgctgtttt
      121 ccagaatttt tcgtcattcg ggttagccag tttagccatt cgttactctc ttcattccaa
      181 tagcattaat tttctctgca ataattgttg taaaaatgtg acgcaaagag gtttttggtc
      241 ataagtaatt accgtcaagt gccgatgact ttctatcagg agtaaacctg gacgagagac
      301 aacggtaatg aatacaactc cctcacagcg attaggtttt ttgcatcaca tcaggctggt
      361 tccgttattt gcctgcattc ttggcggtat cttagttcta ttcgcattaa gttctgctct
      421 ggctggctat ttcctctggc aggccgatcg cgatcagcgt gatgttactg cggagattga
      481 gattcggacc gggttagcga acagttcaga ttttttgcgt tcagcccgga tcaatatgat
      541 tcaggccggg gctgcgagtc gtattgcgga aatggaagca atgaagcgaa atattgcgca
      601 agccgaatcg gagattaaac agtcgcagca aggttatcgt gcttatcaga atcgaccggt
      661 gaaaacacct gctgatgaag ccttcgacac tgaattaaat caacgctttc aggcttatat
      721 cacgggtatg caacctatgt tgaaatatgc caaaaatggc atgtttgaag cgattatcaa
      781 tcatgaaagt gagcagatcc gaccgctgga taatgcttat accgatattt tgaacaaagc
      841 cgttaagata cgtagcacca gagccaacca actggcggaa ctggcccatc agcgcacccg
      901 cctgggtggg atgttcatga ttggcgcgtt tgtgcttgcc ctggtcatga cgctgataac
      961 atttatggtg ctacgtcgga tcgtcattcg tccactgcaa catgccgcac aacggattga
     1021 aaaaatcgcc agtggcgatc tgacgatgaa tgatgaaccg gcgggtcgta atgaaatcgg
     1081 tcgcttaagt cgtcatttac agcaaatgca gcattcactg gggatgacag tagggactgt
     1141 tcgacagggc gcggaagaga tttatcgtgg caccagcgaa atttcagctg gcaatgcgga
     1201 cctgtcatct cgcaccgaag aacaagcggc ggctatcgaa caaactgccg ccagcatgga
     1261 gcaactcact gcgacggtga aacagaatgc ggataacgcg catcatgcca gcaaactggc
     1321 gcaagaggct tctattaaag ccagcgatgg cgggcagacg gtttccggtg tagtaaaaac
     1381 gatgggcgct atctccacga gttcgaagaa aatttctgag atcaccgccg tcatcaacag
     1441 tattgctttc cagacgaata ttctggcact gaatgctgcc gttgaagccg cgcgagcggg
     1501 tgagcaaggg cgtggatttg ccgttgtcgc cagcgaagta cggacactcg caagccgcag
     1561 cgctcaggca gcgaaagaga ttgaaggctt gatcagtgaa tcagtcaggt taattgacct
     1621 ggggtcggat gaggtggcaa cggcagggaa aaccatgagc actattgttg atgccgtcgc
     1681 gagtgtcaca catatcatgc aggaaatcgc cgccgcctcg gatgaacaaa gtagaggcat
     1741 aactcaggtt agccaggcga tttctgaaat ggataaggtg acgcaacaga atgcttctct
     1801 ggtagaagag gcctcagcag cggcggtgtc ccttgaagaa caggctgcac gattaactga
     1861 ggcggtgaac gtattccgtc tgaacaaaca ttctgtgtcg gcagaacctc gcggagcggg
     1921 tgaaccagtt agtttcgcta cggtgtgaaa atgttcaagg agggatcgac agatcccttc
     1981 acctttcaga acggcattga ttttcgaata gcgttaatca tcaactggca accagaagag
     2041 aacgtcgcat ctacgcgggt cagtattcca atcggttcgc ctgcaccatg tcccggaaca
     2101 ggcagggcca ccagcgtggc atgacgcagg tcgtctttta cagcgccaga agggacaaac
     2161 cacacgtaat cgtattcaac ggtaagttga cgagatagcg aagtagacag cgtttcgata
     2221 caacccgaag gaattttaca gccctggctc tgcactaatg catctgaatg ctggcgtggc
     2281 gcagtgcctt ctggtgatac aacgaccggc cattccagca cccggcttag cgttacgttc
     2341 tcctgaagta gcgggtgatt agggcggaca accagcttca acgattcaag aaacagcagt
     2401 tcgtaattaa gcccggtcat cagttcagga tctgacatcc gaccaatgcc gatatcgatt
     2461 tccccggttt ttaaacccgc cagaatcata gggttactca ttgtcgcaac ttgcaaggtc
     2521 gtctcttttt gttgctgatg aaactgacct ataaccgaag gtaatatccc cagtgccgca
     2581 gtaggtagtg caccaaccct gacgacatca ttattaagac cttctttacg atgaagcgac
     2641 tgtccggcag tgttgatggc gtcaagaact ctgactgcat gcgttaaaaa ttgttcgccg
     2701 ggtaaggtaa gttgcgcccc ctgacgacca cgctcaaaca agcgagcgcc cgtcagctgc
     2761 tccagttcat tcaatgtatt agagagcgca ggttgactca aattaagggt ttcagctgcg
     2821 cgccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg caaacggatg
     2881 cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc ggtgtcgctg
     2941 acaagtgaag ttgtttgatt atgataactt gattgcaaaa tattattaac aattaaagca
     3001 attatgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa
     3061 ttcttcccgc cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag
     3121 acactatggc gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt
     3181 cagccatgta ccagcaagaa gttccgcaat atggcacgct gctggaactg gtagctgatg
     3241 tgaatctggc tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagacg
     3301 agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc
     3361 ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc
     3421 tctcgcaggc aggggtgccg gtacattcga cagcatttcg gcccattgat gatgcttctc
     3481 tggcgcgtaa tcccttccgc gtttttacat ccttactccg ccttgagctt atcgagaacg
     3541 aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt
     3601 gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg
     3661 agtttgtgca ggaagccctg gaaacgtttc gctggcacca gttagcaacg gtagatgaag
     3721 aaacctatcg cgccttgcac aacgaacatc ggttgattgc tgatgtggtc tgttttcctg
     3781 gatgccatat caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga
     3841 tgcctgaatg cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac
     3901 cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc
     3961 agaaacaggg cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa
     4021 cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata
     4081 atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt
     4141 taatgcgtca gcaagggttg gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc
     4201 atcgtcaggc gattcatcct ggagacgatc cacagccctt aattgaacgt ggttgggtag
     4261 tggcgcaacc catcacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt
     4321 caaatctggg taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg
     4381 agcaggcgtt gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac
     4441 gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc
     4501 ctgattttac aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca
     4561 tattctctca gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa
     4621 ataagcactt tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga
     4681 ctcactttca ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt
     4741 gtgcggtacc agcggcattg cttctctttt ttctcaggca gcattcgcgg cagattctga
     4801 tattgccgac gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga
     4861 cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat
     4921 gacgccgcag gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt
     4981 tgagaaccgt caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt
     5041 tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga
     5101 gttgttcaaa tacaacgatg caggtgttga taccaaacaa ttagaagggc aaagcgatct
     5161 cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc
     5221 atttctcggc gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc
     5281 ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa gagttccccg actttaccgc
     5341 cttctggttt gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga
     5401 tagcgccagc attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat
     5461 tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcatttcgcc
     5521 gatgaccagt atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc
     5581 gcaaattcat gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg
     5641 tccgctgaat aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg
     5701 gtttggttta ttgcaactgg atcgtgactt ctcccattat caggacatta tgggctggta
     5761 taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg
     5821 cctgatggaa atcccaacaa cgggcgaaac gctggataac attgtctgct tctggcagcc
     5881 agaaaaagct gtaaaagcag gtgatgagtt tgcattccag tatcgtctgt actggagtgc
     5941 gcaaccgcct gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg
     6001 tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc
     6061 cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca aaaggcattg agccggtgat
     6121 tacgctttcc agtggggaag cgaagcaaat cgaaattctc tatattgaac ccatcgatgg
     6181 ttatcgtatt cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg
     6241 gatgtatcta cgttgtcagg gggacgctat cagtgaaaca tggctgtatc agtatttccc
     6301 gccagcgccg gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct
     6361 tcggcacctt cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct
     6421 aagttaaatg agtaatctga tggtgtgtat ttcagataca ccgtgtcagc cactaacagg
     6481 gagtgcgtat gtttccagaa taccgagatt taatatcccg tctgaaaaac gaaaatcctc
     6541 gctttatgtc cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag
     6601 gttccgacgg tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt
     6661 taaaagatga gatgctcaaa atcctgcagc aggagagcgt caaagaggtg taaactttcc
     6721 taagccgcca gccaggcggc ttttttaaca acagcatgga ttgactggag ataagatgac
     6781 tgaaacgata aaagtaagcg aatcacttga attacatgct gttgcggaaa gtcacgtcac
     6841 acctctttat cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc
     6901 gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca
     6961 tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat
     7021 ctcgtttaat cgtattgaac cactgaataa aactgctgaa atcggctact ggctggacga
     7081 atctcatcag gggcagggga tcatttctca ggcgctgcag acattgattc atcattacgc
     7141 tcagtctggt gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa
     7201 ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct
     7261 gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg
     7321 ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa
     7381 ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa
     7441 ttatcaccgc acctgtaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa
     7501 tcggtccacc acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat
     7561 taccttccac aatggcatat tccgccacct gcgaactgta atgaatcgtc ggaatggcat
     7621 cttcttctat gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc
     7681 agacgttgtt ctcttcatta ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt
     7741 caataaaagc ataccggacg acggcatcgc cataaatttg tgcctggtgg acaatacgcg
     7801 aggcgctaac ccttgcgcga tcataaattt ggagcaggag ttgatggtcg ggcgttaagc
     7861 cttgtgcggc gacgatcata gaatgttgat caattaaggc gtgaccaaat attctacatt
     7921 gcacgtaaac taatgagtca tgaatggtga cactgtcact tatatatgca ccctggctga
     7981 tttcactatt atcgatccag acgttatccg ttgcgtaaac ctctccccac agcacgctgg
     8041 tgccggtgat acgcgtatta ccggaaatca ccgttccgcc gaacgcaata gcattctggt
     8101 cgtaaatcca gcaatttcct tcctgtgcta acaccgtttc gcgatcgatc cagccgccag
     8161 cggttcctgc tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacac
     8221 tttttttagt gccatcttcc tgataactaa aggctcgctg ttcttcactt aggcgatatt
     8281 tgcgcatagc gttttcccac aggtgactta ctataaccgt agcaaattct gcggctctgg
     8341 ctatgctcga gaaaattcca taaaatgcat ttcaaatata ctttataaat taaacaaaat
     8401 gagtaagaag atgcagagcg ataaagtgct caatttgccg gcaggctact ttggtattgt
     8461 gttggggacg atagggatgg gatttgcctg gcgctatgcc agccaggttt ggcaggtcag
     8521 ccactggtta ggggatgggc tggtgattct ggcgatgatc atctggggat tattgactag
     8581 cgcatttatt acccgactca tacgctttcc gcatagcgtg ctggcggaag ttcgccatcc
     8641 agtgctgagc agttttgtga gtttgtttcc ggcaacgacg atgctggtgg cgattggttt
     8701 tgttccgtgg tttcgcccac tggcggtgtg catgttcagt tttggtgtcg tggttcagtt
     8761 ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg aagaagctac
     8821 cacgcctgga ctgtatctgc cgacagttgc caacaacttt atcagcgcaa tggcctgtgg
     8881 tgcgttgggc tacaccgacg ccggtctggt gtttttaggc gcaggcgttt tctcatggct
     8941 aagccttgaa ccggtgatct tgcagcgtct gcgcagttcg ggagaattac ccacggcact
     9001 gcggacatca ctcggcattc agctcgctcc tgcgctggtg gcctgtagtg cctggctgag
     9061 cgtcaacggc ggcgagggtg acacgctggc gaaaatgctt ttcggttatg gactgctgca
     9121 actgctgttt atgctacgtc tgatgccatg gtatctctcc cagccattta atgcttcatt
     9181 ctggagtttc tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc tggggagtgg
     9241 cagcgataat ggatttttcc atacgctggc ggtgccgctg tttatcttta ccaattttat
     9301 tattgcaata ctgctcatcc gtacttttgc gcttctgatg cagggaaaat tgttagtcag
     9361 aaccgagcgc gccgttttaa tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac
     9421 tattttactg ataaatatga attaacccgc acacactctg aagtactgga agcggtgaaa
     9481 gtggttaaac cgggtaaaac gctggatctg ggctgtggca atggtcgtaa cagtctttac
     9541 ctggcagcca atggttatga tgttgacgca tgggataaaa atgccatgag tatcgccaac
     9601 gtcgagcgca ttaaatccat tgaaaatctg gataatttac acacccgggt cgttgatctg
     9661 aataacctca catttgatgg acagtacgat tttattcttt cgactgtggt gctgatgttc
     9721 cttgaggcta aaaccatccc agggttgatt gccaatatgc aacgttgcac taaacctggt
     9781 ggttacaacc tgattgtggc ggcgatggat accgctgatt atccatgtac cgtcggcttc
     9841 acgtttgcct tcaaagaggg agaattacgt cgatattacg aaggctggga gagggtgaaa
     9901 tacaatgaag acgtcggcga gctgcaccgc accgacgcca acggtaatcg tattaaactg
     9961 cgtttcgcca cgatgctggc acgtaaaaaa tgacccggta agcacaaaac gcgtgaaaat
    10021 tccccacgct gagatgattt actgttcttc ttttcggtaa gcatattttt tatcgaaggg
    10081 atgtgaaatt aatcacagta gtcgaagttt tttagcagct taacttactg aaatttaact
    10141 actgatgatt gacttagccc ctttttcggc attgactatg tcgtctgaaa aggggctgaa
    10201 aaatttattt tcaccaacac tttttttgcc acaacacgaa gcggcgcttt ttgctataac
    10261 ttagaaagta atataatcat ctcaggaaac tattcatgcg taccacatca tttgcgaaag
    10321 ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc ctctaaaatc acccagccag
    10381 ataaatataa caattactct gatttaaaag aaacaacctc agcaacaggt aaacctgttt
    10441 tacgttgggt agacccgagt tttgatcaaa gcaaatatga cagcatcgtc tggaacccaa
    10501 tcacttatta tccggtacca aaaccttcta cccaggtagg gcagaaagtt ctggataaaa
    10561 ttttgaacta taccaacacc gaaatgaaag aagcgatagc gcagcgtaaa ccagtggtta
    10621 ccaccgctgg gccgcgtagt ctgattttcc gtggggccat taccggtgta gataccagca
    10681 aagaagggct gcaattctat gaagtggttc ctgttgcatt agtggttgcg gggacgcaaa
    10741 tggctacagg ccaccgtacc atggatactc gcctctattt tgaaggtgag ctgattgatg
    10801 cagcgactaa taaaccggtt atcaaagtcg ttcgtcaggg cgaaggtaaa gacctgaata
    10861 acgaaagtac gccaatggct ttcgaaaata ttaaacaagt tattgatgac atggcgaccg
    10921 atgccaccat gtttgacgtt aacaaaaagt agttccagac gcgccatcgt tagatggcgc
    10981 tttttatccg gtgcgccgta aaaccccatc cttcagggcg gggatataag gcgcggtttt
    11041 ccacctgacc aggtgtttgc tgttgttcaa tgtattggcg gatgacggat attggcgcac
    11101 ctccgcagct actggcaaaa tagccaggac tccacaaaac gcctttgtag taatacctga
    11161 ctgcaatatc tggtcgatct cgtcgcagta acctacccga tacgcctttg aggctgttta
    11221 ccagactgga tatcgccagt ttgggaggtt agttaattaa ca
//
LOCUS       DAJTRN010000115        11258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRN010000115 DAJTRN010000000
VERSION     DAJTRN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(217..292)
                     /locus_tag="OO328_003352"
     tRNA            complement(217..292)
                     /locus_tag="OO328_003352"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="OO328_003353"
     CDS             851..3103
                     /locus_tag="OO328_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931799.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4371
                     /gene="csiD"
                     /locus_tag="OO328_003354"
     CDS             3439..4371
                     /gene="csiD"
                     /locus_tag="OO328_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5931800.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4391..5659
                     /gene="lhgO"
                     /locus_tag="OO328_003355"
     CDS             4391..5659
                     /gene="lhgO"
                     /locus_tag="OO328_003355"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5931801.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5682..7130
                     /gene="gabD"
                     /locus_tag="OO328_003356"
                     /pseudo
     CDS             5682..7130
                     /gene="gabD"
                     /locus_tag="OO328_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7138..8424
                     /gene="gabT"
                     /locus_tag="OO328_003357"
                     /pseudo
     CDS             7138..8424
                     /gene="gabT"
                     /locus_tag="OO328_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8662..10062
                     /gene="gabP"
                     /locus_tag="OO328_003358"
     CDS             8662..10062
                     /gene="gabP"
                     /locus_tag="OO328_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5931802.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10083..10745
                     /gene="csiR"
                     /locus_tag="OO328_003359"
     CDS             10083..10745
                     /gene="csiR"
                     /locus_tag="OO328_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5931803.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO328_003360"
     CDS             complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO328_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5931804.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
BASE COUNT         2882 a         2768 c         2812 g         2796 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 aagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggcccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt
     4021 cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca
     4081 gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag
     4141 ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc
     4201 aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc
     4261 tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt
     4321 cagcgtggct atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg
     4381 aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc
     4441 catgcaactg attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg
     4501 cccggcctgt caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac
     4561 gcccggcagc ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg
     4621 cgatcaaaac ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct
     4681 cgaaatggaa cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga
     4741 gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat
     4801 ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat
     4861 cttccaggcc agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca
     4921 taaaaacggc gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat
     4981 tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt
     5041 tattatctgc ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt
     5101 taaccacctg atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac
     5161 ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga
     5221 aggctatcgc aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg
     5281 gattcgccgg gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct
     5341 gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga
     5401 tctccagccc tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat
     5461 tgacgatttt ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc
     5521 ggcagcgaca tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt
     5581 ggcaagccag agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca
     5641 cgccgcattt aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta
     5701 acttattccg ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag
     5761 tcatcgacgt caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg
     5821 cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc
     5881 tcaccgccaa agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc
     5941 aggacgattt agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag
     6001 gcgaaatcag ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt
     6061 atggcgacac cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga
     6121 ttggcgtcac cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag
     6181 ccggtccggc gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt
     6241 tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta
     6301 acgtggtcac cggttcggca ggcgtggtcg gtaatgaact gaccagcaac ccgctggtac
     6361 gcaaactgtc gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga
     6421 aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg
     6481 atgccgatct cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc
     6541 aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg
     6601 aaaaattaca gcaggcgggg agcaaactgc acatcggcga cgggctggag aaaggcgtca
     6661 ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg
     6721 cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact
     6781 tcttccagcc aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga
     6841 cgttcggccc cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag
     6901 ccaatgacac cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct
     6961 tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca
     7021 atgaagtggc cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt
     7081 atggcatcga agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg
     7141 cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt
     7201 gggcaaattc acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgtttaa
     7261 ggccgtgagt atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat
     7321 ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag
     7381 gtgctggctt acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc
     7441 gatttcgcca agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta
     7501 aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac
     7561 gggcgcacgc attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg
     7621 gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc
     7681 gaggatgacg ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat
     7741 atcgccgcca tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca
     7801 gcctttatgc agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat
     7861 gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg
     7921 gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc
     7981 accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc
     8041 ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat
     8101 ctgctgcaaa aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc
     8161 gaaaaacacc cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg
     8221 tttgaagatg gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc
     8281 gcccgcgata agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc
     8341 cttgtaccac tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag
     8401 tgttttgatg aggcgaagca gtagcaccgt ccccatgcag gaggcgacgc tgcgcgtctt
     8461 gtccggccta cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac
     8521 gtgaacagga ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa
     8581 tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac
     8641 acactttcga gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg
     8701 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     8761 gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc
     8821 gccggattac tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc
     8881 gataccggtt cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc
     8941 atcggctggt tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc
     9001 gccatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     9061 accctcgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     9121 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     9181 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     9241 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     9301 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat
     9361 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     9421 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     9481 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     9541 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     9601 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     9661 ccgtacgtgg cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat
     9721 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     9781 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga
     9841 agcgaaattc gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt
     9901 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     9961 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
    10021 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
    10081 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
    10141 attttcaacc ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg
    10201 ttgggccgtt gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
    10261 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg
    10321 cccgcgccaa tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat
    10381 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
    10441 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
    10501 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
    10561 gttatcgatt tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac
    10621 acgatcagca ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg
    10681 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
    10741 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
    10801 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
    10861 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
    10921 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
    10981 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
    11041 tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc
    11101 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt
    11161 tctccggcat ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
    11221 actcagaaag tgtagcagag gaacgcggcg cgttaacg
//
LOCUS       DAJTRN010000116        11184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRN010000116 DAJTRN010000000
VERSION     DAJTRN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO328_003361"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO328_003361"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5931805.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO328_003362"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO328_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5931806.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="OO328_003363"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="OO328_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5931807.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="OO328_003364"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="OO328_003364"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5931808.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="OO328_003365"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="OO328_003365"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5931809.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="OO328_003366"
     CDS             complement(5839..6879)
                     /locus_tag="OO328_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5931810.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="OO328_003367"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="OO328_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5931811.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="OO328_003368"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="OO328_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5931812.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="OO328_003369"
     CDS             7821..8261
                     /locus_tag="OO328_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5931813.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="OO328_003370"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="OO328_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5931814.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="OO328_003371"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="OO328_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5931815.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11184
                     /gene="rsxC"
                     /locus_tag="OO328_003372"
     CDS             9490..>11184
                     /gene="rsxC"
                     /locus_tag="OO328_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5931816.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2598 a         2877 c         2817 g         2892 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgc
//
LOCUS       DAJTRN010000117        11109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRN010000117 DAJTRN010000000
VERSION     DAJTRN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..458)
                     /gene="intS"
                     /locus_tag="OO328_003373"
     CDS             complement(<1..458)
                     /gene="intS"
                     /locus_tag="OO328_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5931817.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(620..694)
                     /locus_tag="OO328_003374"
     tRNA            complement(620..694)
                     /locus_tag="OO328_003374"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Arg,seq:cct)
     gene            complement(768..1700)
                     /locus_tag="OO328_003375"
                     /pseudo
     CDS             complement(768..1700)
                     /locus_tag="OO328_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1994..2749
                     /gene="mlaA"
                     /locus_tag="OO328_003376"
     CDS             1994..2749
                     /gene="mlaA"
                     /locus_tag="OO328_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5931818.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2931..3989)
                     /locus_tag="OO328_003377"
     CDS             complement(2931..3989)
                     /locus_tag="OO328_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931819.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4355..5695)
                     /gene="fadL"
                     /locus_tag="OO328_003378"
     CDS             complement(4355..5695)
                     /gene="fadL"
                     /locus_tag="OO328_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5931820.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6067..6351
                     /locus_tag="OO328_003379"
     CDS             6067..6351
                     /locus_tag="OO328_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5931821.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6531..7841
                     /gene="fadI"
                     /locus_tag="OO328_003380"
     CDS             6531..7841
                     /gene="fadI"
                     /locus_tag="OO328_003380"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5931822.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7841..9985
                     /gene="fadJ"
                     /locus_tag="OO328_003381"
     CDS             7841..9985
                     /gene="fadJ"
                     /locus_tag="OO328_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5931823.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10188..10673
                     /gene="sixA"
                     /locus_tag="OO328_003382"
     CDS             10188..10673
                     /gene="sixA"
                     /locus_tag="OO328_003382"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5931824.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2778 a         2542 c         2747 g         3042 t
ORIGIN      
        1 gcaccgcgat cttcaaatct gcggattact tccagcagtt gcatcggctc aatatcctga
       61 atctcaaggc cgccgatgat gggtaaaatg tcgtcatcaa acatttttgc aagttcagtc
      121 gcatacccta ctgaccagac ttgcttcttg tgttcgtacc attccttgta aatcgcacta
      181 aatgaattgt tgttagacga agactttttc gcctttaccg gatcgatgcc agccgagatg
      241 tctttcctcg cagtccatgc tttatccctt gcctcctgca aagtcataag cggatatttt
      301 ccgacggtca ggattttctc cttaccgtca atcttgtagc gaagctgcca tacctttttc
      361 ccggatacag ggacataaag gtacaggcca ttaccatcga gaaggcggta tggtttttct
      421 ttcggctttg ctgcttcaat ctgcttaacg gtgagcatgg gtaaaaatcc ggtgggtaaa
      481 attattttat ccacttttta cccgtcatgg agtgcggctg tcaacgatct gacgcgaacc
      541 atgacgaact gtgaatatac ggaaggcttg atattcaggg gattttgcgg actggtacgg
      601 atgggagcga actgataaat ggtgtcccct gcaggaatcg aacctgcaat tagcccttag
      661 gaggggctcg ttatatccat ttaactaaga ggacaatgcg gcgagtatac ccgctaatgg
      721 actgcggggt aagtacgctg ccgctcgatt gcttaaaccc tcgccattta tgctgggttt
      781 ttatcatttt tcttaatgtt ttccgcacgt tctgcttttt ggcgtgcttc tgctttacgc
      841 ttgttgctca tgtcgttacg aatctgtgca tgactcatta acgcgaagat aaaggtgccg
      901 ccgcagatgt tccccgctaa agtaggtagt gcgaagggcc agatgaaatc gctccagtgc
      961 agcgtaccgt taaacaccag atagaggatt tcaacagaac cgaccacgat atgggtggtg
     1021 tcacccaggg caataagcca ggtcatcaat ataatcacca caatctttgc cgcacctgct
     1081 gcaggaaaca tccataccat agtggcgatc agccagccgg aaatgatcgc gttggcaaac
     1141 atctcgctgg gggtgttctt catcacatcc atgccgattt tgacaaatgc atcgcgagtt
     1201 tcttcattga agataggcat atattcaaat gcccacgcag caataccagt cccgagaata
     1261 ttacccagca gcacgactcc ccataaccgc ataagtaagc caacgttgct cattgtcggt
     1321 ttttgcatga cgggtagtac cgcagtcacg gtgttttcgg taaataattg ctggcgggcc
     1381 attatgacga taataaaacc aaaggtataa ccgagattct ccagcaagaa gctgcccggc
     1441 acaccttcca gttcggcatg aaatatccct tttgccagta acgaagcgcc catcgacaga
     1501 cccgccgcaa tggctgacca cagtagcgcc attgcgtcgc gttctagctc tttttcacca
     1561 tcctggcgga tatgctcatg aattgccatc gctcgggagg ggagtcggtc ttcatctatt
     1621 tctatttttt tgccgcgctc tttttcttcg ctctaaactt caatttcgtc gctgtgttga
     1681 tcaattttgt cgttgtccat ggtctcttct cttgaattag cacgtatagc taaagcgtag
     1741 cggctttttt gctcgcaact ggcgggagtt actctgaaaa tgtagaaaag gctgcgtttg
     1801 cctttttctg tttctataga atcaagtagc ctacagggcg gcgattacca ggctatgatc
     1861 aaatcagcaa atcagggcgt ctggacatca gttgacgtgc tgttacaatc gcccacacct
     1921 aaacaggcgg atacggtatc gttccgtcat ggatggcaaa ctgcataagc cataaaaaaa
     1981 cagggagaca tttatgaagc ttcgcctgtc ggcgcttgct ctgggaacta cgcttctggt
     2041 ggggtgtgcg agttccggta cagatcagca agggcgttct gacccgttag aagggttcaa
     2101 ccgcaccatg tacaacttca acttcaacgt attagacccg tatattgttc gaccggtcgc
     2161 tgtcgcctgg cgtgattatg ttccgcaacc ggcgcgtaat ggtttgagca actttactgg
     2221 caaccttgaa gaacctgcgg tgatggttaa ctacttcttg cagggcgacc cttatcaggg
     2281 gatggtccac tttacccgct ttttcctgaa caccattttg gggatgggcg gctttattga
     2341 tgttgcaggg atggcgaacc cgaaactgca acggactgaa cctcaccgct tcggtagtac
     2401 gcttggtcat tatggcgtgg gttatgggcc ttacgttcag ttaccgttct acggtagctt
     2461 cacgctgcgt gatgacggtg gtgatatggc ggatgctctt tacccggttc tttcctggct
     2521 gacctggccg atgtctgtgg gtaaatggac gcttgaaggg atcgaaaccc gcgctcagct
     2581 gctggattcc gatggtctgc tgcgtcagtc gtccgatcct tatattatgg tgcgcgaagc
     2641 gtacttccag cgtcatgatt tcatcgctaa tggcggcgaa ctcaaaccgc aggaaaatcc
     2701 gaacgcgcaa gcgattcagg atgatttaaa agatatcgat tctgaataag aaacaaataa
     2761 aaaaggtgag tcgcaaaact cacctttttt gttgttatcc ctcaattacc acaagtaaaa
     2821 ggagataaac cgacaatgac ccggacgtaa tgatgtaaaa atactgatgt ttgtcactga
     2881 ctatttccga tattcaggcg ttgaaaaatc aaaaaactgg aaaaataatt ttaaatatca
     2941 tcaaaattaa cacctagtgc agatagttga ttaatttcat tttgatattt cgtctggcga
     3001 ttttgaagtc ttttttcaac tttatcttcg agattcttaa agtctttgta tttattttca
     3061 ggatggtcaa cgtcaacttt gttagcgaat aaagtagaaa ttatttcttt tacattatcg
     3121 cgtctaaatt ttgcatcgtc cacttgaaaa ttatatctta ttgctcgacc taaacgttta
     3181 aaaacttcgt taggttctct cagtctttcc ccgttgagct cgtttacaac ctgatgatcg
     3241 gaaccagttg caaatgattc tgccatgata gctttactgc tttcaaacca cgctgaagca
     3301 ctcctggcgt tttcactaca atattgggtg agactatcac cattaatacg aattcctctt
     3361 tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt tccgttttta
     3421 tttttaatta caatagcgag agagtcgcct tttttatctt taccgtacca ggtaaataag
     3481 tcagttggtt cgcttttccc gctctcatca aattccgtta ccgtatgtct gagtttgtgt
     3541 gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa tgcgctgaag
     3601 atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt aatgatagta
     3661 cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg gttgtcgata
     3721 ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac tgaggaatta
     3781 ttaatattag atctataagg atcgtcgttt actcgtatgg cgttttttat aagctcaagt
     3841 agatgtttaa tacaggtgct tattccttcc ttaccattaa aaaggttttt gaactcttgt
     3901 actaatccat taatttcatc attgttattt tcattgatgg attctgggta agctgcgctg
     3961 gtattgttga ttgaaataga tggtagcatg attttattcc atgttgattg atttgttaca
     4021 aatatatatt acctaatgaa atagactatc aaaaaacgct catttattta gaacctatct
     4081 ttaatttgag tgtataagcg cacaatagcc aggggggttg agtatcctgg aaatgcagaa
     4141 aagtaaactc tttgagaagg acgagcagaa cgcttgaaaa tatgcatttc ccttcaggtt
     4201 tgtctgtgga taccacttac tgatttgaaa gggcaagctg ccatcaggct gtttgcaaaa
     4261 ctcaggaggc aagcaatgag gaaactactt tcggtgaagt gggggagtta aagtcacctg
     4321 catagcaggt gactttatcc aggcgaacgc gttatcagaa cgcgtagtta aagttagtac
     4381 cgaacagcca ggctttacct tcagactcga actggtatgg gccttcgtta attttcacgc
     4441 tctgaccgtg catataagaa acaccaacgt cgactgaagc atctttatta aatgcgtaag
     4501 tcgtacctgc actcagccag aaacggtcct ggtccggaat ggagatagaa cgattctgag
     4561 ccggaactgg gctgtcatca aaggcgatac cggtacggaa ggtccagtta tcatcgtagt
     4621 aataagtggt acccaacgcg atgcggtaag catctttaaa gccttcatgt ttctggaaca
     4681 gcgtgtcgcc actggttgag gtcgctttca gctgctggaa ctgactccag ctggtgtaag
     4741 ccaggctata gtgaatcgcc cactgtggat caacacggtt ataacctgac acttcccaca
     4801 tttcaggcag gttcagcgtc agataacccg attgcgttgc gccacctgtc gcggtaggaa
     4861 ttggcaaacc gtagttatta aacgcacgat taagatcgct gctgtagtta cctttgaagt
     4921 caattttcac ttcagaacgg taggtcagtg catagcggtt atttttatcc agttcataca
     4981 ggattccggc gttccagcca aagccccact ggttaccgtt cagatgagcg attttggtat
     5041 tactgtcgat accgttggcg gtagctgcca atgcttgccc ttgaggagtt tgcccggcag
     5101 gagattgcat aatctgacca gcaaccaact gccccagatc gcctgcgaaa cgttcaattt
     5161 tcgcgcgagc gtagatggcg ttgaaaccaa gaccaaagct ccatgcatta tttaagcgat
     5221 acgcaccgct taagttcagg ttcatggttt caaggtcagt tgtacccccg acagagccgc
     5281 ctgcataagt atcattaaac tctgttgcca gaccatagtt agaggtaata gaagcgcccc
     5341 aaccaaattg gtcgttaatc ggtgcaacaa agtgcatgtt cggaacccat gccgtaggcg
     5401 cgatgttatc ggctttcagg ctacgaccag acggagacgt tccgctgata ttcacatccg
     5461 ggtcaatata aaccgcaccc gcagaaaatg tcgggcggtc aaacatggta atcaatgcgg
     5521 ggttacggct aacgttacct gcatcatcgg caattgcgcc ttcccctgaa taagcccggc
     5581 ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc cgaccaggcc tgggtggaga
     5641 taagtgccac tgcgactgcg agagcagact ttgtaaacag ggttttctgg ctcatgacca
     5701 taacctcaat gatttatttt tatacaaact atgttacgtg ctgtaacagg agcgcgaagt
     5761 gtagggtctg aggtacatct tagaaatcag accagtggcg agagtatagg tcggaccagc
     5821 tagaatgttg caagtatgtt tccatctttt ttcaaatatg atctaagaaa cgcgatccag
     5881 ctggcaaaat ttggaggttg cgcactcgcg gattcgggtg aattttgttt tagatcattt
     5941 ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt aacaactgga gcagcacttt
     6001 ccggaagcgt aaaatatcca caacgtttat ctggcacctt gggtgcacat acagaggaaa
     6061 tcaacgatga gtaaatgtag tgctgatgaa accccggttt gctgctgtat ggatgttggc
     6121 accattatgg acaactccga ctgcaccgcg tcttacagcc gcgtgtttgc aaaccgcgca
     6181 gaagcagaac aaacgctggc ggcgctgact gaaaaatccc gtagcgtgaa atccgaaccg
     6241 tgcaaaatct ccccgacttt cactgaagag tctgacggtg tgcgcctgga tatcgatttc
     6301 acttttgcct gcgaagctga aatgctgatc ttccagctgg gtctgcgtta attcctctcg
     6361 ggtacgtacg cccctgtcag ttgctggcag gggcgttttc ttttctttct tctgtcttca
     6421 ctgtgtcata gctcccactt tcctttttct ccgcttggct aaatgtaaaa aaatggttaa
     6481 gactatgatc aggtcagacc actttattta tttttttaca ggggagtgtt atgggtcagg
     6541 ttttaccact ggttacccgc cagggcgatc gtatcgccat tgttagcggt ttacgtacgc
     6601 cttttgcccg ccaggcgacg gcttttcatg gcattcccac ggttgattta gggaagatgg
     6661 tggtaggcga actgctggca cgcagcgaga tccccgctga agtgattgaa cagctggtct
     6721 ttggtcaggt cgtacaaatg cctgaagccc ccaacattgc gcgtgaaatt gttctcggta
     6781 ccggaatgaa tgtgcatacc gatgcttaca gcgtcagccg cgcttgcgct accagtttcc
     6841 aagcggttgc aaacgtcgca gaaagcctga tggcgggaac tatccgagcg gggattgccg
     6901 gtggggcaga ttcctcttcc gtattgccaa ttggcgtcag taaaaaactg gcgtacgtgc
     6961 tggttgatgt caacaaagct cgaaccatga gccagcgcct gaaactcttc tctcgcctgc
     7021 gtttgcgcga cttaatgccc gtaccgcctg cggtagcaga atattctact ggcttgcgga
     7081 tgggcgacac cgccgaacaa atggcgaaaa cctacggcat cacccgagaa cagcaagatg
     7141 cattagcgca ccgttcgcat cagcgtgccg ctcaggcatg gtcagacgga aaactcaaag
     7201 aagaggtgat gactgccttt atccctcctt ataaacaacc gcttgtcgaa gacaacaata
     7261 ttcgcggtaa ttcctcgctt gccgattacg caaagctgcg cccggcgttt gatcgcaaac
     7321 acggaacggt aacggcggca aacagtacgc cgctgaccga tggcgcggca gcggtgatcc
     7381 tgatgactga atcccgggcg aaagaattag ggctggtgcc gctggggtat ctgcgcagct
     7441 acgcatttac tgcgattgat gtctggcagg acatgttgct cggtccagcc tggtcaacac
     7501 cgctggcgct ggaacgtgcc ggtttgacga tgagcgaact gacattgatc gatatgcacg
     7561 aagcctttgc agctcagaca ctggcgaata ttcagttgct gggtagtgaa cgttttgctc
     7621 gtgaagtact ggggcgtgca catgccactg gcgaagtgga cgatagcaaa tttaacgtgc
     7681 ttggcggttc gattgcttac ggacatccct tcgcggcgac cggcgcgcgg atgattaccc
     7741 agacattgca tgaacttcgc cgtcgcggcg gtggatttgg tttagttacc gcctgtgctg
     7801 ccggtgggct tggcgcggca atggttctgg aggcggaata atggaaatgg catcagcgtt
     7861 tactcttaat gttcgtctgg acaacattgc tatcatcacc atcgacgtac cggatgagaa
     7921 aatgaatacc ctgaaggcgg aatttgcctc gcaggtgcgc gccattatta agcaactccg
     7981 tgaaaacaaa gagttgcgcg gcgtggtgtt tatctccgcg aaaccggaca attttatcgc
     8041 cggagcagac atcaacatga ttggcaactg caaaacggcg caagaagcgg aagcactggc
     8101 gcggcagggc caacagttga tggcggagat tcatgctttg cccattccgg ttatcgcggc
     8161 tattcatggc gcttgcctgg gcggtgggct ggagctggcg ctggcgtgcc acggtcgcgt
     8221 ttgtactgac gatcctaaaa cggtgctcgg tttgcctgaa gtacaacttg gattgttacc
     8281 cggttcaggc ggcacccagc gtttaccgcg tctgataggc gtcagtacag cattagagat
     8341 gattctcacc ggaaaacaac ttcgggcgaa acaggcatta aagctggggc tggtggatga
     8401 cgttgttccg cactccattt tgctggaagc cgctgttgag ctggcgaaga aggatcgccc
     8461 atcttcccgc cctctacctg tacgcgagcg tattctggcg gggccgttag gtcgtgcgct
     8521 gctgttcaaa atggtcggca agaaaacaga acacaaaact caaggcaatt atccggcgac
     8581 agaacgcatc ctggaggttg ttgaaacggg attagcgcag ggcaccagca gcggctatga
     8641 cgctgaagct cgcgcctttg gcgaactggc gatgacgccg caatcgcaag ctttgcgtag
     8701 tatctttttt gccagtacgg acgtgaagaa agatcccggt agtgatgcac cgcctgcgcc
     8761 attaaacagc gtgggaattt taggtggtgg cttgatgggc ggcggtattg cttatgtcac
     8821 tgcttgtaaa gcggggcttc cggtcagaat taaagatatc aacccgcagg gcataaatca
     8881 tgcgctgaag tacagttggg atcagctgga gggcaaagtt cgccgtcgtc atctcaaagc
     8941 cagcgaacgt gacaaacagc tggcattaat ctccggaacg acggactatc gcggctttgc
     9001 ccatcgcgat ctgattattg aagcggtgtt tgaaaatctc gaattaaaac aacagatggt
     9061 ggcggaagtg gagcaaaatt gcgccgctca taccattttt gcttcgaaca cgtcatcttt
     9121 accgattggt gatatcgccg ctcacgccgc gcgacctgag caagttattg gcctgcattt
     9181 cttcagtccg gtggaaaaaa tgccgctggt agagattatt cctcatgcgg gtacatcggc
     9241 gcaaaccatc gccactacgg taaaactggc gaaaaaacag ggcaaaacgc ccattgtcgt
     9301 gcgtgacaaa gccgggtttt acgtcaatcg catcttagcg ccttacatta atgaagctat
     9361 ccgcatgctg accgaaggtg aacgggtaga gcacattgat gccgcgctag tgaaatttgg
     9421 ttttccggta ggcccaatcc aacttttgga tgaggtagga atcgacaccg ggactaaaat
     9481 tattcctgta ctggaagccg cttatggaga acgttttagc gcgcctgcaa atgttgtttc
     9541 ttcaattttg aacgacgatc gcaaaggcag aaaaaatggt cggggtttct atctttatgg
     9601 tcagaaaggg cgtaaaagca aaaaacaggt cgatcctgcc atttacccgc tcattggcgc
     9661 acaagggcag gggcgactct ccgcaccgca ggttgctgaa cggtgtgtga tgttgatgct
     9721 gaatgaagca gtacgttgtg ttgatgagca ggttatccgt agcgtgcgtg acggggatat
     9781 tggtgcggta tttggcattg gttttccgcc atttctcggt ggaccgttcc gctatatcga
     9841 ttctctcggc gcgggcgaag tggttgcaat aatgcaacga ctggccacgc agtatggttc
     9901 ccgttttacc ccttgcgagc gtttggtcga gatgggcgcg cgtggggaaa gtttttggaa
     9961 aacaactgca actgacctgc aataagaagg tcaaagctat atgaatccgc gctgaatggc
    10021 ggagtgttgg tcaaaatgta aacgcatatt gactatactt acgccattga ggtaaaaaac
    10081 agcgtttcat tcggtgaatg gataaggcac aatgccggcc accgttttct ttctctggtt
    10141 tcagatgaaa gaaaacgggc gaatctggtt aacaaaagcg gtgcaatatg caagttttta
    10201 tcatgcgtca cggcgacgca gccctcgatt ccgccagtga ttcggttcgt cctctgacca
    10261 ctaatggttg tgacgaatct cgcctgatgg cgaactggct gaaaggtcaa aaagtggaaa
    10321 tcgaacgcgt tctggtgagc ccgttcctgc gagccgagca aacactggaa gaagtagggg
    10381 attgtctgaa cctgccttcc agtgcagaag ttctgccgga gttaacgccc tgcggcgatg
    10441 ttggtctggt cagtgcctat ttgcaggcgt tgaccaatga aggtgttgct tcggtactgg
    10501 tgatctctca cttaccgtta gtcgggtatc tggttgccga gttatgtccg ggcgaaacgc
    10561 cgcctatgtt taccacctcg gccattgcca gcgtaacgct tgatgaaagc ggaaacggta
    10621 cattcaactg gcagatgagc ccgtgtaatc tgaagatggc aaaagctatc tgatgtgttc
    10681 caaattatgc ccaatgaggt agattcattg ggcataattg ctcattaggc agcggatatc
    10741 tgtcaacctc actactatcg aagactctcc ttatctataa ataaaacact ttctacattc
    10801 attcggaaat tattcttcta cgcgtatctt gattttgccc aaagttacat gttgaaatga
    10861 ataatatctg tttgacactc attgtcattt attaatacag ataatagaga aaatagagtt
    10921 gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt gtttatatat
    10981 tatattgtca aaagtggata aatgccaatc gcattatatg ttcattgttt gttttttaag
    11041 ctgttgatat tcattattga tatactttct attttttcta aatacttatt ccgtattttt
    11101 taaggatgt
//
LOCUS       DAJTRN010000118        11048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRN010000118 DAJTRN010000000
VERSION     DAJTRN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            410..1240
                     /locus_tag="OO328_003383"
     CDS             410..1240
                     /locus_tag="OO328_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5931825.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="OO328_003384"
     CDS             complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="OO328_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5931826.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2369..3829)
                     /locus_tag="OO328_003385"
     CDS             complement(2369..3829)
                     /locus_tag="OO328_003385"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5931827.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="OO328_003386"
     CDS             complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="OO328_003386"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5931828.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5434..6777
                     /gene="gntP"
                     /locus_tag="OO328_003387"
     CDS             5434..6777
                     /gene="gntP"
                     /locus_tag="OO328_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5931829.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="OO328_003388"
     CDS             complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="OO328_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5931830.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="OO328_003389"
     CDS             complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="OO328_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5931831.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="OO328_003390"
     CDS             complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="OO328_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5931832.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9006..>11048)
                     /gene="fimD"
                     /locus_tag="OO328_003391"
     CDS             complement(9006..>11048)
                     /gene="fimD"
                     /locus_tag="OO328_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5931833.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2685 a         2827 c         2683 g         2853 t
ORIGIN      
        1 tacgtagatg aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt
       61 ccgcataatc agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa
      121 catatcaaat acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc
      181 aatatcaaat tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa
      241 tgagcgtttt ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga
      301 tatcactaca acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat
      361 ttatctattt ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc
      421 attaagcaat gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa
      481 agtcgacagt gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta
      541 tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat
      601 tagaaaactt aatattggca aacagtcctg gccatttaat tatgtgacag atacatgcag
      661 agaattcaat ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc
      721 agctttagac gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc
      781 agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac
      841 agttcatgaa ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat
      901 aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag
      961 aattgatatc tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt
     1021 cgcccttgcc gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat
     1081 agaagcctat cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg
     1141 tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt
     1201 tgaattaaaa aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac
     1261 taatattcag gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat
     1321 tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag
     1381 tcaggcgtcg actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc
     1441 gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc
     1501 cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc
     1561 caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg
     1621 attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt
     1681 tgcttcagca atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct
     1741 ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg
     1801 ggtagcctgc agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa
     1861 cagctcaaaa ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct
     1921 gcctgagcta tcaagcacat agataccggc accccggcgc acttccacca gccctttgat
     1981 ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat
     2041 ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgtg
     2101 gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt
     2161 gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg
     2221 cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag
     2281 ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga
     2341 ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc
     2401 gttcgcacag ctgctgatat gccgacgtca ccgcgccaac aaagtcggca ttctgcggca
     2461 gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat
     2521 actgcgcgtt aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac
     2581 cctgctcatc cacgccctgg gtgtaaccca tccagccagc cacgcccagc gccaggtgac
     2641 gccagctgcc gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact
     2701 tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac
     2761 gctcgatcag cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg
     2821 gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg
     2881 tgtcggcaat ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc
     2941 cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgtgc
     3001 ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga
     3061 acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta
     3121 aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt
     3181 tctcttcaat ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga
     3241 ccttcgccac atggccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca
     3301 gccctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg
     3361 cagtcgggtt ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg
     3421 ccgcatcagc gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac
     3481 gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca
     3541 tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca
     3601 gttgctgttt tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt
     3661 cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt
     3721 gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat
     3781 cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca
     3841 gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc
     3901 cggtgggtat taacggataa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc
     3961 ggccaggcct ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc
     4021 gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc
     4081 tttacgacgg tgttcttctt caacaatcgc tttcaccact ttgtacatat caacgtcacc
     4141 gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag
     4201 atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc
     4261 acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc
     4321 aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg
     4381 cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat
     4441 cgctttcagg aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag
     4501 ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc
     4561 aatgatgtta cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc
     4621 tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg
     4681 catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag
     4741 cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt
     4801 gcgaatgcca cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc
     4861 atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca
     4921 caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac
     4981 ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac
     5041 atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt
     5101 tcctctttca catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt
     5161 atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt
     5221 tctacctttt ggtaagacat gtcaaccaga attctgaaat tggttaaccg cgtcgcaaga
     5281 atttcactct catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc
     5341 agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa
     5401 taacaacggt tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg
     5461 gtattcggca ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg
     5521 gctttgttgg tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg
     5581 ctgcacacca tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg
     5641 ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat
     5701 acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcctt
     5761 attttcggtc tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt
     5821 gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct
     5881 gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct
     5941 tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta
     6001 atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca
     6061 ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt
     6121 ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg
     6181 gtaaaagata cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg
     6241 tttattgcga tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag
     6301 tgggtgatga acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc
     6361 ggtgcgggtg gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc
     6421 atgttgatgt cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta
     6481 attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt
     6541 gctgcaatcc ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta
     6601 ctggcgacgg ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg
     6661 ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     6721 ctggtcaact ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag
     6781 aacaccagag cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg
     6841 gacagtcccc tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg
     6901 cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg
     6961 tgcaggtttt gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt
     7021 aattttttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     7081 cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta
     7141 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     7201 cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     7261 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     7321 gaggaattgg cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa
     7381 catcacaacc gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact
     7441 gaaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac
     7501 cagctttaat agccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     7561 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg
     7621 gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag
     7681 ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa
     7741 agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt
     7801 ttacataaac attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac
     7861 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag
     7921 ctcgtttcat tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact
     7981 gcctgaatgg ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg
     8041 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     8101 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta
     8161 taatatccgg tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     8221 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata
     8281 agactgaaag aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac
     8341 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     8401 cttgcgagcg ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc
     8461 tccagttact gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca
     8521 ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc
     8581 agggtcgtcc acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca
     8641 ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg
     8701 ttggcattgt ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta
     8761 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta
     8821 aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca
     8881 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg
     8941 cgacttaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     9001 cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc
     9061 agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt
     9121 cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc
     9181 tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc
     9241 gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga
     9301 gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta
     9361 tccagcgcca ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc
     9421 cagtcggtac gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta
     9481 acaagcacca ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat
     9541 accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata
     9601 ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta
     9661 ccatcgcctc ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc
     9721 aacgtaccgt atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg
     9781 tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa
     9841 ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc
     9901 aggctatagc tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat
     9961 tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt
    10021 gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg
    10081 aggttgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg
    10141 ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa
    10201 taacggtaac ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga
    10261 aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc
    10321 atatccacag acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca
    10381 cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg
    10441 agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct
    10501 cctgccgtaa tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag
    10561 ggtacggtaa aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca
    10621 ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta
    10681 taaatgccat atccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg
    10741 atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt
    10801 gcgccgcgaa agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc
    10861 agccgggaac gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta
    10921 tttttgctac ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa
    10981 cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc
    11041 ccaatccg
//
LOCUS       DAJTRN010000119        10814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRN010000119 DAJTRN010000000
VERSION     DAJTRN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..285
                     /locus_tag="OO328_003392"
     CDS             <1..285
                     /locus_tag="OO328_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5931834.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWM
                     ENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OO328_003393"
     CDS             296..1036
                     /locus_tag="OO328_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5931835.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OO328_003394"
     CDS             1095..1481
                     /locus_tag="OO328_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5931836.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OO328_003395"
     CDS             1490..1819
                     /locus_tag="OO328_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5931837.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..4832
                     /locus_tag="OO328_003396"
     CDS             1791..4832
                     /locus_tag="OO328_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5931838.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4832..5161
                     /locus_tag="OO328_003397"
     CDS             4832..5161
                     /locus_tag="OO328_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5931839.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5161..5859
                     /locus_tag="OO328_003398"
     CDS             5161..5859
                     /locus_tag="OO328_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5931840.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5864..6607
                     /locus_tag="OO328_003399"
     CDS             5864..6607
                     /locus_tag="OO328_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5931841.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6544..7146
                     /locus_tag="OO328_003400"
     CDS             6544..7146
                     /locus_tag="OO328_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5931842.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7207..10686
                     /locus_tag="OO328_003401"
     CDS             7207..10686
                     /locus_tag="OO328_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5931843.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10754..>10814
                     /locus_tag="OO328_003402"
     CDS             10754..>10814
                     /locus_tag="OO328_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCR5931844.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2545 a         2560 c         3466 g         2243 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgcgcag tacaccggaa aaatgacgga tgaggacgac
       61 tggcaggctg ttctgcatat tgccgttttc gtccgtgcac aggcgccgga ttctgagctg
      121 gatacgtgga tggagaacac catatttccg gccatgaagg atattccggt gctttccgga
      181 ctcatcgaca ccatgatccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     4621 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     4681 agtacaacat tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga
     4741 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     4801 atggtggccg gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc
     4861 ggatatgaat gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga
     4921 acagcgtcgt gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt
     4981 tcgtaaaggt gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5041 atttcagtgg acgccacctt atgactggaa actgatcagg gtggtttgtc gtaaatggtc
     5101 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     5161 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg
     5221 gtggagctct gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat
     5281 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc
     5341 attcaggggt cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg
     5401 gtctctaacc tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc
     5461 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
     5521 ggaaacagcg acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc
     5581 agcgaactga gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc
     5641 gcggttttcc cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat
     5701 gagtgtggtt acacgggcag ggcagtggct gacgagttcg acaaaccaac aacggatatc
     5761 cggaaggaca aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat
     5821 ttcggcggtt tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc
     5881 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggct tcgtggtgag
     5941 aacgccggag ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg
     6001 gttccggatg gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact
     6061 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
     6121 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcacaagt tccgctgtgt
     6181 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     6241 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
     6301 gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
     6361 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
     6421 tcatgccgcc atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag
     6481 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     6541 ggaatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt
     6601 cgtgtgaaaa cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt
     6661 cagaaactga gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc
     6721 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg
     6781 cgtctggtgg gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg
     6841 gcggttgcta tatggatgcc gggggtagga attatggcga gtaatctgct gttttctctc
     6901 ggtgccagta tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc
     6961 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
     7021 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg
     7081 gtatctcagg agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt
     7141 cgctgatgca aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg
     7201 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
     7261 aaatccacgc agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg
     7321 gtggatggat taaaaagcgt gctgctgaac ggtacgccgg tcctggatag cgaggggaag
     7381 acaaactttt ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg
     7441 gaggggtttg aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg
     7501 ccgatcaccc ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg
     7561 caggcactgg tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     7621 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     7681 accacttcgc agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat
     7741 atccggatgc gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc
     7801 tggtcgtcgt acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc
     7861 ggcgtgcagg tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt
     7921 cgcgggcgca ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt
     7981 atctgggacg gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac
     8041 atgctcactc atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa
     8101 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc
     8161 acggagccgc gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt
     8221 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     8281 ttcgtgcagg accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg
     8341 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc
     8401 gttgaggtga actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag
     8461 gacacgcagg ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     8521 accagccggg gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa
     8581 acgcagaccg tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc
     8641 attgaaatct gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg
     8701 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc
     8761 acgctgataa gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc
     8821 accgacggcg tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg
     8881 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     8941 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc
     9001 gtggataacg gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg
     9061 ccagcggtgc agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg
     9121 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     9181 gcagcggacg acggcagtga gcggctggtc agcacagcca ggacgacgga aaccacatac
     9241 cgcttcaggc agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg
     9301 gggcagcagg gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc
     9361 actattgaac tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat
     9421 gacccgacgg tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag
     9481 gttgaaacca gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat
     9541 atcaggccgg gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg
     9601 gcatttgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct gagtttttat
     9661 aaagggttga tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt
     9721 cagcttgcgc cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc
     9781 acgcaaaccg tcaataaaaa actggaaaat cagagtgctg caatccagca gatacagaaa
     9841 gttcaggttg atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg
     9901 aaggacggac gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga
     9961 atgcagagtc aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc
    10021 aacacaaagc cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg
    10081 aaacgcctga cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca
    10141 cctggcggac ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc
    10201 gggacgctca acaacgtcac gattaataag aactgtcggg ttctgggaaa attgtccgcg
    10261 aaccagattg aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg
    10321 gcaccggaga ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt
    10381 gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat
    10441 aacaatattt attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac
    10501 cgaacgaccc tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac
    10561 ggtcacatga cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca
    10621 gcaagtatca gcgatttgct ggttgttgtg atgaagaaat ccacagcagg tatcagtatc
    10681 agctgaattt tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa
    10741 gcgagaggta attatgcgta aactttatgc tgccattttg tccgcagcca tctgtctggc
    10801 tgtatccggt gcgc
//
LOCUS       DAJTRN010000120        10741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRN010000120 DAJTRN010000000
VERSION     DAJTRN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            587..784
                     /locus_tag="OO328_003403"
     CDS             587..784
                     /locus_tag="OO328_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931845.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(734..1927)
                     /locus_tag="OO328_003404"
     CDS             complement(734..1927)
                     /locus_tag="OO328_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5931846.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2063..3787
                     /gene="iucA"
                     /locus_tag="OO328_003405"
     CDS             2063..3787
                     /gene="iucA"
                     /locus_tag="OO328_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5931847.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3788..4735
                     /gene="iucB"
                     /locus_tag="OO328_003406"
     CDS             3788..4735
                     /gene="iucB"
                     /locus_tag="OO328_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5931848.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4735..6477
                     /gene="iucC"
                     /locus_tag="OO328_003407"
     CDS             4735..6477
                     /gene="iucC"
                     /locus_tag="OO328_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5931849.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6474..7811
                     /gene="iucD"
                     /locus_tag="OO328_003408"
     CDS             6474..7811
                     /gene="iucD"
                     /locus_tag="OO328_003408"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5931850.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7817..10012
                     /gene="iutA"
                     /locus_tag="OO328_003409"
     CDS             7817..10012
                     /gene="iutA"
                     /locus_tag="OO328_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5931851.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10563..10706)
                     /locus_tag="OO328_003410"
     CDS             complement(10563..10706)
                     /locus_tag="OO328_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931852.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2649 a         2722 c         2823 g         2547 t
ORIGIN      
        1 aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa tcatgactcc
       61 tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag ttttacagga
      121 tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc ccaaaaaaca
      181 taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt caggtgtttg
      241 cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca gtaaaaatac
      301 ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa aagaaacctg
      361 gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc ctgataccca
      421 tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac gcgcagcgca
      481 gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca gagcaacgct
      541 gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg agattacacc
      601 gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca gaacactgcc
      661 tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact ccagtaaggt
      721 gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac acaatggtaa
      781 ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct gttttcgata
      841 ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta gcgagccagc
      901 ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg ggtgttgcca
      961 gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc tgttcggaga
     1021 gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg gccaccggag
     1081 tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact gcggacagac
     1141 ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac tggcgagtaa
     1201 gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat attgcgcaaa
     1261 gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt gtgggctgtg
     1321 gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct gccatcagca
     1381 gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc ggcccgaata
     1441 gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga ttcccttctc
     1501 cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg gtcagcccgt
     1561 aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg gcgctggtag
     1621 ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct cccagagccc
     1681 acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac ggggacgatg
     1741 gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga ctcagatttg
     1801 tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata ccgagaagcc
     1861 cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct tcgatccctg
     1921 gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa attaatttat
     1981 ctgacaataa catttattgt tgataatgag aatcattatt gacataattg ttattatttt
     2041 actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca cagatgtggc
     2101 tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac tgacagaata
     2161 cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt tcagagtggc
     2221 ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg tcacggcatc
     2281 aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata agctacgcca
     2341 tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc tggaaagcca
     2401 tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc gtgaaaaagc
     2461 gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc accctgcgcc
     2521 taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg atatggcacc
     2581 ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg aaagtttgca
     2641 tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc agttactcaa
     2701 cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg aatatctttt
     2761 gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact taggtgaggc
     2821 cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca ccagccgcga
     2881 tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc tgtccgtgaa
     2941 agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct ggcagatgct
     3001 acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg ggctgctcga
     3061 tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc tgctggtgga
     3121 tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac ctgatggcgg
     3181 tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta tcactgtgca
     3241 acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc cgctgtttac
     3301 ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc ttgtccagat
     3361 gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg cttttatgcc
     3421 tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata ttttcaccca
     3481 tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt ttgctgtgac
     3541 cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc gtgtacgagc
     3601 atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact acgtgctgga
     3661 aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc ataacgagaa
     3721 caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc aggctcagga
     3781 ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct gtgaaaaact
     3841 cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc actgtcccgg
     3901 ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg ccgcaccaca
     3961 actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg cgctgacgct
     4021 ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt taccgttatg
     4081 gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag agcgtgagat
     4141 ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt acgatccacg
     4201 tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag aacgtttcac
     4261 ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct cactggaggt
     4321 acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc tgattgggtg
     4381 cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag accgcattgg
     4441 tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg gtgaacagca
     4501 atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt acctgctgct
     4561 ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc agcgcctgtt
     4621 ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc cacataagcg
     4681 ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc tgtaatgaat
     4741 cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc tgagatggag
     4801 tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat taacctgccg
     4861 ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg gattgatgcc
     4921 caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat gcagctaaag
     4981 ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt gtatgccacg
     5041 ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag tgacctgatt
     5101 aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt cgtttttaat
     5161 aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga gtataccaac
     5221 accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg ctgcgataac
     5281 gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt tacacgcttc
     5341 agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc ggtacatccg
     5401 tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga aggcaggatg
     5461 gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg taccctgacc
     5521 aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta caatacctca
     5581 tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc acgctggctg
     5641 caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat ccttggtgaa
     5701 ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc tccctatcgc
     5761 taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct gaaaccggat
     5821 gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca gccgctggca
     5881 ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca attgttccgg
     5941 gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct tattgcacac
     6001 ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct gctgaaagac
     6061 ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc tttgcctcag
     6121 gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga tttgcagacg
     6181 ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct tggcgtacct
     6241 gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa caaacatcca
     6301 caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat tcgcgtggtg
     6361 ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat gctgccgaat
     6421 taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga atcatgaaaa
     6481 aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt gctgcattgt
     6541 cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat ttttcctggc
     6601 atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa gatctggtca
     6661 gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag cacaaaaagt
     6721 tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt tctgactatc
     6781 tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt gaaaatattg
     6841 atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa tattttgccc
     6901 acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg aagcatgtga
     6961 cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg agtggaaaac
     7021 ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat gcattacgcg
     7081 gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat tttaacgcac
     7141 tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca ggcttctccg
     7201 gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca tcggatggca
     7261 tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt gaagttctga
     7321 gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg gaaagtagtg
     7381 gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag agcctggaaa
     7441 gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata cttccctcac
     7501 tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat gacttcactc
     7561 tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc atgcaaactc
     7621 atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt attcttaatc
     7681 gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt cagtggcgca
     7741 gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat acagcaaata
     7801 ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc cgctgcttct
     7861 tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca tagtgtctgc
     7921 cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta tcgaaaatgc
     7981 cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg ctcagttaat
     8041 ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca tgcgtggccg
     8101 cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg acagccgaca
     8161 actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg gcgcgacggc
     8221 cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa aaggtcagcc
     8281 ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca gtaaagatca
     8341 cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg gacgtctttc
     8401 cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca ccctgcttga
     8461 taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa ccggtacgct
     8521 gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa gtcagggaga
     8581 cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga gcagcacacc
     8641 atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc atttgatcag
     8701 cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg tttactaccg
     8761 cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac aggcgacggc
     8821 tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc tgaacagcca
     8881 actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc gctttacctc
     8941 caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga acaaccataa
     9001 gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg ccttcctgca
     9061 atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct atcagtatac
     9121 tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg ctaccgggaa
     9181 ggcgatatct gccgacgcca ttcctggtgg ttcggtagac tacgataact ttctgttcaa
     9241 tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt tttcccaggg
     9301 ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg cagcagtgaa
     9361 cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg aaggcgtgaa
     9421 agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga ctcaaatcgc
     9481 ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga ccatcagtgt
     9541 gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc tgatcccgga
     9601 tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga aagtgaacgg
     9661 tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag cttacattaa
     9721 ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg acgtaagcga
     9781 tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta gttggcagct
     9841 tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact ataccactgt
     9901 ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt cactgtacga
     9961 ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct gacggggatg
    10021 cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt tgtttacagc
    10081 cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt tcaccactga
    10141 ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc ctcatttcat
    10201 tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct ggcagatcct
    10261 gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg ctgcaatgaa
    10321 ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa aaagaggtac
    10381 ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct gtcataaagt
    10441 ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc cggcccggag
    10501 gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg ctataggcaa
    10561 agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt ctgaataacg
    10621 cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat cgctaattcg
    10681 tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg aaatattaaa
    10741 c
//
LOCUS       DAJTRN010000121        10721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRN010000121 DAJTRN010000000
VERSION     DAJTRN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1194)
                     /gene="rspA"
                     /locus_tag="OO328_003411"
     CDS             complement(<1..1194)
                     /gene="rspA"
                     /locus_tag="OO328_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5931853.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLED"
     gene            complement(1400..1726)
                     /locus_tag="OO328_003412"
     CDS             complement(1400..1726)
                     /locus_tag="OO328_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5931854.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1861..2202
                     /locus_tag="OO328_003413"
     CDS             1861..2202
                     /locus_tag="OO328_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5931855.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2268..2797
                     /gene="speG"
                     /locus_tag="OO328_003414"
                     /pseudo
     CDS             2268..2797
                     /gene="speG"
                     /locus_tag="OO328_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2800..3510)
                     /locus_tag="OO328_003415"
     CDS             complement(2800..3510)
                     /locus_tag="OO328_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5931856.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3618..3923
                     /locus_tag="OO328_003416"
     CDS             3618..3923
                     /locus_tag="OO328_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5931857.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4122..4442
                     /locus_tag="OO328_003417"
     CDS             4122..4442
                     /locus_tag="OO328_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5931858.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5002..5580
                     /locus_tag="OO328_003418"
                     /pseudo
     CDS             5002..5580
                     /locus_tag="OO328_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6266..7255)
                     /locus_tag="OO328_003419"
     CDS             complement(6266..7255)
                     /locus_tag="OO328_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931859.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYVLQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7340..7831)
                     /locus_tag="OO328_003420"
     CDS             complement(7340..7831)
                     /locus_tag="OO328_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931860.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8152..8652)
                     /locus_tag="OO328_003421"
     CDS             complement(8152..8652)
                     /locus_tag="OO328_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931861.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8642..9157)
                     /locus_tag="OO328_003422"
     CDS             complement(8642..9157)
                     /locus_tag="OO328_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931862.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9588..9956)
                     /locus_tag="OO328_003423"
     CDS             complement(9588..9956)
                     /locus_tag="OO328_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931863.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10368..>10721)
                     /locus_tag="OO328_003424"
     CDS             complement(10368..>10721)
                     /locus_tag="OO328_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5931864.1"
                     /translation="HSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTPSAYSSW
                     FNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDLYGKG
                     HAVGRLADVLKTALL"
BASE COUNT         2762 a         2716 c         2653 g         2590 t
ORIGIN      
        1 atcttccaga cgtgcgactg gtagataagc aggttcatag ggatatttcg ccgccagctt
       61 ttcatcgaat tcgataccaa gacccggttt gtctcccgga tgcatatagc cgttatcgaa
      121 agtccagttg tgcgggaaga cttcgagcat ttgttcggaa taacccatgt attcctggac
      181 accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga ctggtgacaa
      241 atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat cggcaatccg
      301 gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg cggatataat cgatgagttg
      361 ctcttcaatc agttgtttgc agtcccagat gctgttgaag acttcaccca ctgcgatggg
      421 tgtgacggta tgttggcgaa tgagacggaa gcattcttgg ttttccgctg gcgtcgggtc
      481 ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg cttcaatagg
      541 cgttaagcga tggtgcatgt catgcagcaa atgttcatta aaaccaaact tgttacgtac
      601 cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca gctgctcttc
      661 cggccactgt cctttggttg cgggttcata agccagacct ttacctttcg acatgccgta
      721 ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc ccagctcctg
      781 atgacgggca taatcatcca gagcttcatc aatactgtga ccggtggtat ggcaataaac
      841 catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt tggcagcttt
      901 ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg taaccggacc
      961 gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac ggtgcgcatc
     1021 gcgaccaata agccgcggac aaagatgatc ctgcaaataa gaggccacgg aaagctcacg
     1081 tccattgagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga tttttaatgt
     1141 gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct tcatgttcga
     1201 ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa ctgagctact gccatacaag
     1261 tatgaagatc gaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg cgctgtgaat
     1321 ggttgacaaa agatgaaata gaataccttt tgtcagctga cacttcctcg tatcttattg
     1381 ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc aacaatgatc aacattccgc
     1441 aaagcgcaat caacgcaccc gtccagtcat aaagacttag tttcacgcca tccacaacgc
     1501 gcagccacat caatgccgtg cagacataaa cgccaccata agccgcgtaa acacgcccac
     1561 tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc cagtgaaatc cccgccggaa
     1621 gcaacagcca gatactggcg tttcgtttta accacaacca gggcagaaag catccaataa
     1681 tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt gtcacttatt
     1741 gacatcatgt atagttatag ggcgacataa tatcatcaat ataaacatcc tcctggtacg
     1801 ttttgcgttc gcagtggatg gtgtagaatc acctttaatc attcatacag ggaatgaatt
     1861 atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc ttgcgcactg
     1921 gcattgagca caactgttca tgccgaaact aacaaactgg tgattgagtc tggcgacagt
     1981 gcacaaagcc gccagcacgc cgctatggaa aaagagcaat ggaatgacac gcgcaatctg
     2041 cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc cgcttttgat
     2101 aaccgcgata aatgtgagca aagcgccaac atcaatgcct actgggagcc caatactttg
     2161 cgctgcctgg accgtcgaac tggccgcgtt attaccccct aacctgttat tgatttaagg
     2221 aatgtaagga cacgttatgc caagcgccca cagttaagct acgcccgctg gagcgtgaag
     2281 atttacgcta tgtacatcaa ctcgacaata acgccagtgt gatgcgttac tggtttgagg
     2341 aaccctacga agcctttgtt gaactctctg atctgtatga taagcgcata ttcacgatca
     2401 gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg tggagctggt
     2461 ggaaattaac catgttcatc gccgggcaga atttcagata attatctccc cggagtatca
     2521 ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg gactatggct tcaccgttct
     2581 caatctctat aagctgtatc tgatcgtcga taaagagaat gaaaaagcga ttcacattta
     2641 ccgcaagctt ggctttacgg ttgaaggtga attgatgcat gagtttttta ttaatggtca
     2701 atatcgtaat gccattcgca tgtgtatatt ccagcatcag tatctggcag agcacaaaac
     2761 accgggtcag actctcctga agccgaccgc acaatagcat taataataat cgatcgtatt
     2821 tttgatggtg taaacccgtt cgacggcggg ttttactcct tcatcaacaa tgatcagctg
     2881 acagtccact ggattggcgt gactgtcata ttcacagctc tgttttacat taccaactcg
     2941 ttgattattt agtaaagtaa ccgctgtgta atctaatttt ttgatcggat ccgttgatgg
     3001 cgtggcgctg acagataatg ttttgtcgtt acttttcgtg gttttaccca gcggataccc
     3061 ctgatcatca tagcgatatt ccatctgcat ttgtttgctg ctggctttaa tcacgaagcc
     3121 attatcatcg gtttcccaac tcaccccggc agaaggtaat tctgctagct ggcattttcc
     3181 ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg taataattgg cgtccagtac
     3241 cagagcgacc acggtattat tttccagatc cagtaattcg agtgaatcaa aacagccttc
     3301 ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct tgctcatcca ttaatgtctg
     3361 agtgaaatct tttaccggac cacgcagcgg atcaaaatcg aattcattag agaaactggc
     3421 catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga ggaatatcgc
     3481 gagtaagcat ggtagtaatt tgtatttcac aacagtcacc agagagtaga gatgattctc
     3541 aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag aagttatctt
     3601 cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg ctcgccctcg
     3661 cctcacccgc ggtactcgcc gcgccgggat cttgtgagcg catacagagc gatatatcac
     3721 agcgcattat caataatggc gtacccgaaa gcagcttcac gttaagtatt gtacccaatg
     3781 accaggttga tcagcctgat tcccaggtcg tcgggcattg cgctaatgat acgcataaaa
     3841 ttctctacaa ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct acccaggatg
     3901 gcgcgcctgc cgaaccgcag taacacatta tcgactgaac gccggatatg acacatccgg
     3961 cgatttgaac atacaacata atctcacctt attactcata cccttctatt gatgtggatt
     4021 aataattctt aacccaaaat gggtagactc cctctattgt tagcgcgcta aatattcaat
     4081 gtataaacat ttatataaca ataaagaaca gggagtgagt tatgtccaaa aatgaccgaa
     4141 tggtgggcat cagccgcaga acactcgtta aatcaaccgc gacaggttct ctggcgctgg
     4201 ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc agcggcagcg gtacaacagg
     4261 ccagcgaaaa agtgatctgg ggttcctgtt ccgtcaactg tggtagccgc tgtgcacttc
     4321 gtctacatgt taaagataat gaagtgacct gggtggaaac tgacaatacc ggcagcgatg
     4381 agtacggcaa ccatcagggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     4441 aatcatcccg atcgcttgaa tgacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     4501 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     4561 gtcgaacaat atggcaatga agctgtatat attcagtact cttcggggat tgttggcggc
     4621 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     4681 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     4741 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     4801 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     4861 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4921 acggctgccg gtcgtgaaga ggaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4981 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     5041 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     5101 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     5161 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     5221 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     5281 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     5341 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     5401 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     5461 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     5521 ttcatctgga actacaagga cttgaagcgg acccttagta ggcctgaggt cctgcattga
     5581 gtcaagacct tgtgtcattc tttgtgccag acttgtgtca ttagacggga tgtctagacg
     5641 gctggtcagg accctccggt ggcctcggtc tggacttgag gcgggctatg ggggaagtct
     5701 gctggcttaa tgtattagat ggtcattcac atttttctgt cagaaaccat cggtcccact
     5761 gtgagtacaa acctaagccc ccagaagccc acccacagcc ttagacagag tgtcgaatac
     5821 attcacaccg agtcgaccgg agaggaccat atagaggccc acagagagga acacgagttt
     5881 atagaccaga gtcaggaggg accttaggtc ttcggaagtc atggacataa agcccccccc
     5941 tatcttctat tgcactaccg cactagacaa ctagtacaaa acaaaggccc agaccattgg
     6001 tccagaccgt agttagttaa tcacagtgac aagccaccac ctgaagtcct gctgatattc
     6061 agtccagact tacctctgct catggtctta cctccctcgg tctcctgacc ttgggtatcg
     6121 actacagctt gctgagcgtc atctggagga gctacaggag gagctacagg aggcgggacc
     6181 gaagccccac cttgagtccc accaccacct gtcaggccct tggcgatgga cctgaagcct
     6241 ttactaatgc tcttacccat tggtcttacg cttcggtctt ggtgaacttc aggcgtttga
     6301 ctacaggcag gacggcagta gctgagccag tgcccatctt gatggtcgta gcagcttcag
     6361 cgacaacgaa cactttaccg aggatttcca agtcgtggga ctgagagtcg ccatcacgtg
     6421 aaaggacgac cgtaatattc tcaccgtcgt gacggaagca gccgaagctc agggccgtga
     6481 agcgtttagc cagcatgaat acacccttgt cttcaccgtt gtaataggcg atgtctgtcc
     6541 ccagcatatc gacgatagcg tcttcaccgg agaagccttg cttagcagcc agacgggaca
     6601 gtgctttcag ttggctctca tgagcgataa taccgtagtc agacacctca gaaccgaaag
     6661 aagactctgt gttctcatac agagcgtcca tgatagcgtc tgccttagcg gtcaggctac
     6721 cagtcaggac aggaagctct acggtcttca gcagcttaga ggattccaga acgtcgacca
     6781 tgcgagcgtt gatggtacgg accacattct cacagatgtt catagcgtcc tgctgaagtg
     6841 tgccctgagt gaatccggtg gatgacttgg cgtcccagag cttggtctgc tcagagatga
     6901 gaatagcctg ctcgattggt tcgaccacga tttcttgtcc agacggaacg gtcacgactt
     6961 caggtcgctt gccaccacgg acaggaacga ccacagagcg gtcagccaga gtgtgagcaa
     7021 tgattggggt cggtaagctg ttacgggtcg gcaggacttc tacagggaac gggatgttct
     7081 cttgagcgtc tttaagtaca ccgttcagca tagccttaac cgtcttagct aactggagga
     7141 catagacctg cgggtcagcg ttagtcggca gaccttcatt gacctgaatg ttgtcggtat
     7201 cggtgatgcg tactggctca atgccaaact ggaaagcgtc tttaatcgta gtcataataa
     7261 tcaatctcct aaattagttt aaatactgtg agtcctcaaa actgaggagt ccctaacatc
     7321 gggtcaggga ctgtgtgttt tacaagctgt ccatccagtt accgcctgac tcaaggtcag
     7381 cgacaatagc ggcacgggag cgaccagcgg aatgggtagc actgcggagg ctctcaccat
     7441 aggtccggcg ttcagctttg acctgttcgc ggcgctgtag ctctttctgc cactctgcat
     7501 cggtcatgtt ctcttcacgc atacgctcag cctgaagtcg ttgctcttta gcgatacgct
     7561 cagcttcagc tcgtttgttc atctggcggc gaccataggc ggtcatcaag tcattactga
     7621 agaagttgcc aatggtcacg ccagcttcag ccagagcttc cagtaactcc attgcctcgc
     7681 ggctggtaga cggcaggcct gacaggtagc ccggaatgag ggtcttgata gcgagcgaca
     7741 gcggataggg ctgaccgtcc agactcacag tgttttcata aaggttgatg tcggtgactg
     7801 acagcttcca gttgttgttg ttgaacatca taggtaattc tcctcttgag agttgagact
     7861 atcggtatcg cagacgcagt agcgcctacg ggtctccccg catcggtcat ctcaaaaggt
     7921 ttaaaattag gtttggtatg gtcaggactg cttagcaaag gctcggcgta actgattgtc
     7981 taaatcacgg tccaccagca tagctatcag ctcgtctcga ctgtagtaag ggcgctcagg
     8041 gtcttcgact ccaagtctca gcagagacag cagatgacgt gtgggcaggc cacggtaacg
     8101 aagggtcgct ttgccgatag gtgtacgagg gtcaatcata gccatgatta cttacctcca
     8161 ctcacagcga ccagcggtca cagcttgata gtctgaatac tcagcctgtg agacttcacg
     8221 tccggcgatg tagaggcctg tgccatgacg ctggatgaat tccttggtct ccggcttgat
     8281 gtcctcagca gacgctacag cagactgcac ggtggcgctg gaggtcatag acacgaaggc
     8341 tcgggttact gccagtccgt acccaaggtc caaccaccga ccgaactcta tagcggctac
     8401 tgggtcagcc agccacgagg tccctaagtg acccatgtta atctttttaa caaacccgtt
     8461 ttctgggttg gttagacttc taccagcctt ggtcctcatg aagcgagcaa cctcggtact
     8521 acggttatct tctaccttac caatctgaat gcctacccgg cgtatctcat ccagtcgaaa
     8581 caatccgtcc tgactggtga tgtcctgacc gaagatatga atggtgtatt cctgcttatt
     8641 gtcagaagtc ataagaggca cctcccgcca gagcttcggc aaaggacttg aagcgctgtt
     8701 cttggtcagc tttcgcctga aggcgttcgg catcaggtgg gaccagctta tcaaaggtgc
     8761 cgttttcgta atcttcagtt aggacctgaa ggcggcggga ccatttgctc tccacctgca
     8821 tcatcgcgtt agagatgtgc ttcaagtcgg catcagagcg tgacgctatc caatccatct
     8881 cttctgagtc accctcacag gtgtaaggat tgttgaaata gaagatgtcc tgcaggccac
     8941 gcttcagctc ttcctcggtg tgtgtctgga ggtatgactg gataggctcc gcgtacatct
     9001 cccggatttt ctcatagtcc cagtcttccg cctgggcata ccatcgggat aacttcagca
     9061 ggtctaagtt atagcgacag caatcgacac tggactctgc attggtcagc aggatgatta
     9121 agaactggcg gttagttact ggtaattcgg tattcataca aagatgctcc tttggtaaag
     9181 cactttctgt ctccacatgg agtccgcacc attcagacgc atcctaaacg gacccatcag
     9241 aaagtgctta acgaaagtga atggttaaat gttgacggtc tggagtgacc atgttctcca
     9301 tgtacgtata aaggtgcgac cgcatgtcag gacgagctgt aaggactcca gatggtgagc
     9361 ggtgatagac ttaagccaat gtagtgagta gacgagctac taaccgagac gactaaagaa
     9421 cgctaacgag ctggaggtcg atacaggagg tctgtcagag gcggtcacgg gccttgatta
     9481 cttaggagag atacttaatg tcggttgacc agatgctcgc atgttctgac gcatcagact
     9541 cagaggcgga ggctggtctc cctatagtga tagtttaggg tccgtaatca tgaaccagct
     9601 ttactggcgt ggtactggac ggatttactg gcgctgacga cggtagcata gacgtagcga
     9661 gcgtagcgag ttgaaaccat aggttcaccc aaggcaacac gcttacggct cagggactcc
     9721 tgcacagcgc ttacagttaa tgtgtcgtgc gttagtagtt gggtcagaat caagtatggg
     9781 ctgacaggct tggtattgct tgccattgat tcgttcaggc gaccagcacc agtaaggaca
     9841 tcatccagcc aaccactcag gtcgccctga ccatcgaatg acagcaggtc cccgcagtgc
     9901 tgctgcaagt aggctttggt gaacgtgttg tcgtgagttg cgattggtcg tgccatagag
     9961 tatctcctgt agttgaatta gttggttccc ctatagtgat aatcaagtcc atcactggtg
    10021 atgcatacag tagcgcgaca ggatgtgact cacaggttga accgaatgtc aaggactaaa
    10081 gacgcctcag gaattaagga agtatatata aagagagacc agaggcgact cacagttaaa
    10141 caggaatgtc aagaggagac cataggaatt tggagggact ggagtttagg acgcagttta
    10201 aataagacca cagacagact tagagtcgga cagccgaagg cggaccagtg ggcctatagt
    10261 caggagggat tatgattatt atcaccctcc ctaacttcag tcagacttca gtgacccaag
    10321 gtctcaacca tcaagccgta tatggtctcc accttgggtc tccctactta aagtaaagct
    10381 gtcttaagca catcagccag cctacccaca gcgtgtcctt tcccgtacag gtcatacgta
    10441 attgactgac tgctgtgacc taagatgcct tgagcgtcca ccagattgac tccacgctct
    10501 ttcattcggg tcgccagcca atgtctcaga ctgtggagag agacatcctg agagtctcct
    10561 agggcctcag tcaggaccct tgagttaaac cagctagagt aggcgctcgg agtcatcccg
    10621 aagagaggac cctcatcagg ctgcatgtct acccacgaca ggaaggacgt caggtcaaac
    10681 ccataagcgc catcggttaa cggcacaagt cggactgaat g
//
LOCUS       DAJTRN010000122        10433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRN010000122 DAJTRN010000000
VERSION     DAJTRN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..270)
                     /locus_tag="OO328_003425"
     CDS             complement(<1..270)
                     /locus_tag="OO328_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5931865.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OO328_003426"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OO328_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OO328_003427"
     CDS             complement(1130..1336)
                     /locus_tag="OO328_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5931866.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OO328_003428"
     CDS             complement(1443..2006)
                     /locus_tag="OO328_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5931867.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="OO328_003429"
     CDS             complement(3381..5216)
                     /locus_tag="OO328_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931868.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="OO328_003430"
     CDS             5317..5604
                     /locus_tag="OO328_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5931869.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="OO328_003431"
     CDS             5576..7105
                     /locus_tag="OO328_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5931870.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="OO328_003432"
     CDS             complement(7275..8492)
                     /locus_tag="OO328_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5931871.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="OO328_003433"
     CDS             complement(8859..9845)
                     /locus_tag="OO328_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931872.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10370)
                     /locus_tag="OO328_003434"
                     /pseudo
     CDS             complement(9842..10370)
                     /locus_tag="OO328_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3250 a         2386 c         1964 g         2833 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctacgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg
    10141 cgcattgcct gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt
    10201 ttaaccggtt cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc
    10261 gttcccccaa taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct
    10321 gattccgtaa tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc ctgatcttac
    10381 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg act
//
LOCUS       DAJTRN010000123        10346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRN010000123 DAJTRN010000000
VERSION     DAJTRN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..152
                     /locus_tag="OO328_003435"
     CDS             <1..152
                     /locus_tag="OO328_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931873.1"
                     /translation="VVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAMTALLMK
                     DNQRS"
     gene            254..1708
                     /gene="amn"
                     /locus_tag="OO328_003436"
     CDS             254..1708
                     /gene="amn"
                     /locus_tag="OO328_003436"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5931874.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2051..2767
                     /locus_tag="OO328_003437"
     CDS             2051..2767
                     /locus_tag="OO328_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5931875.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3223..3298)
                     /locus_tag="OO328_003438"
     tRNA            complement(3223..3298)
                     /locus_tag="OO328_003438"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3263..3265),aa:Asn,seq:gtt)
     gene            complement(3399..5026)
                     /gene="yeeO"
                     /locus_tag="OO328_003439"
                     /pseudo
     CDS             complement(3399..5026)
                     /gene="yeeO"
                     /locus_tag="OO328_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5031..5106
                     /locus_tag="OO328_003440"
     tRNA            5031..5106
                     /locus_tag="OO328_003440"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5064..5066,aa:Asn,seq:gtt)
     gene            complement(5144..6094)
                     /gene="cbl"
                     /locus_tag="OO328_003441"
     CDS             complement(5144..6094)
                     /gene="cbl"
                     /locus_tag="OO328_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5931876.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6196..7113)
                     /gene="nac"
                     /locus_tag="OO328_003442"
     CDS             complement(6196..7113)
                     /gene="nac"
                     /locus_tag="OO328_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5931877.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7441..7516
                     /locus_tag="OO328_003443"
     tRNA            7441..7516
                     /locus_tag="OO328_003443"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7474..7476,aa:Asn,seq:gtt)
     gene            complement(7572..8507)
                     /gene="ldtA"
                     /locus_tag="OO328_003444"
     CDS             complement(7572..8507)
                     /gene="ldtA"
                     /locus_tag="OO328_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5931878.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8569..9648)
                     /gene="cobT"
                     /locus_tag="OO328_003445"
     CDS             complement(8569..9648)
                     /gene="cobT"
                     /locus_tag="OO328_003445"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5931879.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9660..>10346)
                     /gene="cobS"
                     /locus_tag="OO328_003446"
     CDS             complement(9660..>10346)
                     /gene="cobS"
                     /locus_tag="OO328_003446"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5931880.1"
                     /translation="RWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCGAPL
                     AALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVV
                     LAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGR
                     QTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIEL
                     GELVFLLALL"
BASE COUNT         2888 a         2616 c         2242 g         2600 t
ORIGIN      
        1 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
       61 ggaactggca tagcatcgcc atttatttgc tggctggatg cctgatttct gcaatgaccg
      121 ctttattgat gaaagacaat caacgctcgt gatagcctgg cgaagatcat ccgatcttcg
      181 ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
      241 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
      301 actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg gcaaatatat
      361 tacaattggc gaattacctg atgaaaacgc ccgtaaacaa ggtctttttg tctatccatc
      421 actgaccgta acctgggacg gtagcacaac aaatcccccc aaaacgcgcg catttggtcg
      481 ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta
      541 tctgaatgag caactaacgt tgctttatca ggattatggt gcgcatatct ctgtgcaacc
      601 ctcgcaacat gaaatccctt atccttatgt catcgatggc tctgaattga cacttgatcg
      661 ctcaatgagc gctgatttaa ctcgctactt cccgacaaca gaactggcgc aaatcggcga
      721 tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc attttgatgc
      781 gcgccgcgtc gatttttccc tcgcacggtt gcgccattat accggtacgc cagtagaaca
      841 ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg
      901 gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt gtgctggcgg
      961 gaactggatc actgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa
     1021 aaaacaccag atgccagcat ggcatttaat taccgctgat ggtcagggta ttactctggt
     1081 gaatattggc gtgggaccgt ccaatgctaa aaccatctgc gatcatctgg cagtgctacg
     1141 cccggatatc tggttgatga ttggtcactg tggcggatta cgtgaaagcc aggccattgg
     1201 agattatgtc cttgcacacg cttatttacg cgatgaccac gttcttgatg cggttctgcc
     1261 gcccgatata cctattccga gcattgctga agtgcaacgt gcgctttatg acgctaccaa
     1321 gttagtcagc ggcaggcctg gtgaggaagt caaacagcgg ctgcgtacag gtactgtggt
     1381 aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc gttttaactt
     1441 aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt
     1501 ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttacatg gtgagattaa
     1561 gcttcccggc caggctaacc gtttttatga aggcgctatt tccgaacacc tgcaaattgg
     1621 cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg
     1681 aacctttaat gagccgccgt tccgataata agttatgaac cttcttatca gaaatagatg
     1741 tgaaggatta agtaagacat ctggcaaata gcctgcaata aacgcgggct gcttgcttcc
     1801 ttgggcagat gcgagtttta ttatcgtctt aatgatttcc acatattaaa agcaagtatg
     1861 ctttcaaaac acaattataa aaaatcccgc caacaacatt agtttttata aaattaaata
     1921 taagattatg gctttagaat atttttattt ctaatagacg agatttttcc tgttatgata
     1981 taatatgccg aattaacaca tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga
     2041 gatcattcca gtgggacgta aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg
     2101 tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc tatgctgctg ccaaacaagg
     2161 tgaacccgat ccagaattaa acacatcttt aaaattcgtt attgaacgtg caaagcaggc
     2221 acaagttcca aagcacgtta ttgataaagc aattgataaa gccaaaggcg gcggagatga
     2281 aacgttcgta cagggacgtt atgaaggctt tggtcctaat ggctcaatga ttattgctga
     2341 gacattgact tcaaatgtta accgtacgat tgctaacgtt cgcacaattt tcaataaaaa
     2401 aggcggcaat atcggagcgg caggttctgt cagctatatg tttgacaata cgggtgtgat
     2461 tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt ttgcttgaag ctgaagttga
     2521 tgttcgtgat gtaactgaag aagaaggtaa tattgttatt tatactgaac ctgctgacct
     2581 tcataaagga atcgcggcgc taaaagcagc tggaatcact gagttctcaa caacagaatt
     2641 agaaatgatt gctcaatctg aagttgagct ttccccagaa gatttagaaa tctttgaagg
     2701 gcttgttgat gcccttgaag atgacgacga tgtacaaaaa gtttatcata acgtcgcaaa
     2761 tctctaatta tattttaaag aaatctgtct ttatggcaga tttctttaat ctcatataat
     2821 tcttataaaa aatataatat ttaactcgtc atattgctta taaaaaatcc ccgtccccag
     2881 agaaataata tttattaaaa tggcaggtct tctttttctg attaccgaag acaaagtggc
     2941 aattacgtat agtttcccga taaagacgcg acagctacgt cccgcataag gcatttttct
     3001 ctttatcttt gtacggtact tcatggaaca gaatttttga ccttgtgaat cgtgatatct
     3061 gttggggagg ggcaatttgc tcactgaagc gtgagactcg attaagcgca cgaaacacag
     3121 aaatcaaaaa acccggccac ttttttacaa agtggccggg taaaaataat ttttattttt
     3181 taactgtcct gagtctcata gagatgtctc aaaactaaaa tttggctcct ctgactggac
     3241 tcgaaccagt gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat
     3301 cgtgagaacg aggcgaatat tagcgatgcc cacccacaat gtcaaagcct gttttttaaa
     3361 tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt attccgacac aactggcttt
     3421 ttttcacact tttgcggctc ggatcgaggg tatttccaca gccaacgtcc agtaaccatt
     3481 cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa acattcccat ccagacacca
     3541 accacacccc agccaagcat gattccctgc acataaccga ctacaacccg gcaaccccac
     3601 atgctcaaca tagaaaccca catggcgtaa cgggcatcac gagcaccctt aaatccagcg
     3661 ggtagcaccc atgaggcgga ccaaataggc ataaataaag catttagcta aatcagaatc
     3721 acaacgacgt gtttaacctg tggatcctgg gtgtaaaacg atgccataac cccggcaaag
     3781 ggagccgtta gccaggcgat ggccgttaat ccaagagtgg acagccagaa cacatgccgc
     3841 aactgaatct ctgcttgcgc tatctgccct acccccaacc ttcggcctgt aatgatcgta
     3901 gaagcagaac cgagcgcact tccgggtaag ttgataagag ccgcaattga gaacgcgata
     3961 aaatttccgg caataacgct ggtccccatc ccggcaacga acatttgggt taataaccga
     4021 ccactggtaa ataacactga ttcgacactc gctggaatac caatccccat gacttcccag
     4081 ataatgctaa aattcagcgg tttaaaatag ctctttaacg aaatccttag cgcaggatta
     4141 aaaccaatcg ccagcaccca caaaattgca actgcgccaa tataacgaga aatggttaaa
     4201 cccaacccag ccccgaccag gagaaaaggc cgtaaatcaa tatgccgcta ataataatat
     4261 taagaatatt caggctaccg ttaatcagta gcggtatttt cgtattccct gcaccacgaa
     4321 gtgccccgct accaataaga gtgatggcag cggctggata actgagtacg gtcagctcca
     4381 gataagtcaa cgccagtgct ttaacttctg tcgtggcatc acccgcgacg aaatcaataa
     4441 tttgttcgcc aaaatgatga attagcgttg ccaacagtac ggcaaacaac gtcatgatca
     4501 ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg agactaaatg
     4561 ctacaacgac agtagtacca agatcgatag ctgcaaaaaa agccataatg accatattga
     4621 agctgtccgc caaccccacg ccggccatcg catcttttcc cagccaactg accagaaaag
     4681 tgctcaggac ccccatcaac aggacacagg cgttctccat gaagatagga acagcaagcg
     4741 gggttatctc gcgctagaac aacactttgt agctcttgcg tttagagtgc cagcgagtgc
     4801 cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat tgcagaaagt
     4861 gaaaccacat ttcaaatgat gagggagaat cagcaaagct gcaaagattt tcgccaacaa
     4921 attgtctgca aatgcaacaa actgttgata gaaacggcaa acagttggga aatttaaaaa
     4981 tcgggtttga caaaatattt ttcgccgtta agatgtgcct caacagcgat tcctctgtag
     5041 ttcagtcggt agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag
     5101 gagccaaatt tctgaaaagc ccgcttttat agcgggcttt ttgctatatc tgataatcaa
     5161 tttcctcttc actgttttcc atcacctggc gcttgatatc ctcaactgac agtcctgcat
     5221 tacaaagttc cagaaagcgc caaacatagt tacgctgaag ttgtcctcgc ttcagtccca
     5281 accagacagt attagcatcg aaaagatgcc gcgtatccag gcggattaaa ttctcttcct
     5341 cttgttcgcc gctggattgt tcggcaacta atccgatccc aagcccaaga gcaacatagg
     5401 ttttaatgac atcagaatcc tgtgcactta atacaatatc tgccggcaaa cctttgcggg
     5461 taaatgcgtc atcagtgcgt gagcgccccg taattccctg tcggtaagta attaacggcc
     5521 acttcgctat tgattccagc gtcaatggtg taatttgcgt taagggatga tcgagtggaa
     5581 caagcaaact atggtgccaa caaaaccatg ggaaggcgac gagctgcggg tcattactca
     5641 aacgctcgct ggcgatacca atatcagctt cgccattttg caacaacgtc gcaatttcct
     5701 gtggcgaacc ctggattagc tcgagccgaa cctccgggaa aagttcgcga aaagctttaa
     5761 tgacctctgg caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg agaacaccag
     5821 acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt actggcttca ttcagaatac
     5881 gttctgcaat gaccagtaat gctttgcccg gttcagtcat ccccagcagt cgcttacctc
     5941 gtcgaacaaa tatttcgatg ccaaattcat cctccagttc ccgaatatga cggctaacgc
     6001 ctgactgtga ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc tgacgtgcag
     6061 cctcgcggat tatctttagt tgttggaaat tcacggtaaa ctccgggcag ttcagatatc
     6121 ccgttattgt taaagtctaa tgcccggcat aacaaataat aaaaacccgc atcttattcc
     6181 atcccgatat aacgcttagc tcaccaattg ccactgcctt ttttccatca ctggagaact
     6241 aatcactgac attaacaact ctttcactgc ctgtgcctgt ggtgacaagt ttgctctggc
     6301 gggtaaattt aaggacaaag agagactcat tgaaggagtg gtaatgcgtg acatccaccc
     6361 atttactgcg ccacataacg aacgcgcagc cgattcaggt aatactgcaa cgcccatgcc
     6421 gctggcaatc gctgcggtaa gcgtggcaat agactcaatt tcaccaatga cttttgccgt
     6481 gagccgccgt agggaaaaag cctcatcaac acgaagtcta acagcgctgt aatcactggg
     6541 gagaaaaagg ttcatttgcg caatagcatt aacatcaacg ttttgccccg ggcaatcttg
     6601 agttcctacc aggaaaagat cttctttcag caaagcctga ctggataccc cagccacagg
     6661 ggaatgctca taaatcaccg ccatatcgag ttggtgattt atcagttttt cgttaagcac
     6721 tgcaccacta ttttcatgaa gatagataac gacctccggg aattcagcgc gaaccgcctg
     6781 taataagggc atggtgatgg atgacgcagc ggttcctggc gcaaagccaa tcgagacttg
     6841 ccccgataat gactgaccaa cgttatgcac cgccagttgg gcctgctcac actgacgtaa
     6901 aatggcccgt gcatgggtat agagaatttt tccggcgtct gttggcgtaa cgccccgttt
     6961 tgtgcgaatc aaaagctgtt gatttaactc accttccagt atggcaacct gttggctgag
     7021 cgctggttgt gcgatatgca atacttcagc agcctgggtc aggctaccaa tatctacaat
     7081 ttttacgaag tatttcaggc gtctgaagtt catgttgcct ccggttttta agaatcggcc
     7141 caagtgccgc cattacttac aaccagtatg caagatgcct gccagttcta ttttggcgtt
     7201 gatgcgcaag ctaaccaact gtcaaataag agattatgat agattcgtca ttggcccttt
     7261 ttatcagcag tcgcattcac ctgccctata aaaggagggt atgcaccacg atggttcatt
     7321 acccaataag attgaaagct caccactttg ttgaaattgg cagcaaacaa acaaaaaaaa
     7381 tgcatttcac cctttgacat caccatgcac tgccattaat atgcgccccg ttcacacgat
     7441 tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac tggttcgagt
     7501 ccagtcagag gagccaaatt caaaaaagcc tgctttcgag caggcttttt actttctaat
     7561 taccaacgct cttaaaacat ctgtcttgaa ccagaactaa tttgcacagg cattcccgat
     7621 cgatgttgca acgcagcatt tgcgcgattt acatcaactt cttgcccgtt gataaacgcc
     7681 cgcaaagatg gggttaccgg caatggcact tttcggtcag actcatattc tgcacgattg
     7741 cgcgacagcg gctcatgaac ttccagccac tttgaaccat ccggttccgt tgtgtatttc
     7801 actggctgat caataatttg tacacgagtg cctacaggaa cattatcaaa cagatatttg
     7861 atatcgtcat tacgcagacg aatacagccc tgacttacac ggagcccaat accaaaattg
     7921 gcattggtac cgtggatggc atacaacctg ccaatataaa tcgcgtacag ccccatgggg
     7981 ttatcgggcc ccgcaggaac aaatgcgggc aaactctccc ctcgtttcgc atattcgcgc
     8041 cggatgttcg gcgttggcgt ccaggttggt gcttcttgtt tacgttcaac ggtagtcacc
     8101 cagttacgcg gggtttctcg cccagcctgg ccgataccaa taggaaagac ttccactgta
     8161 ttactgtctg gcggatagta ataaagacgc atttcagcga cgttaaccac aatcccttta
     8221 cgaacagtgg cgggcaaaat cagttgctgc ggaatggtga gttgtgagcc aggcttcggc
     8281 aaaaaaacat cagcgcccgg attcgcttcc agcatattac ttaacccttg cccgtattgt
     8341 gcggcaaaag tctccagcgg ctgagtattg tgatcaggaa cagttacagt aagcgactgc
     8401 cccactaaac ggctaccctc tggaggtaat ggataagtta ccgccaggct attttggctg
     8461 gcaaaaagca gagcaaatga gcaaagaata tttacacgac gcatcatgtc cctttcctaa
     8521 gtcgcgaata ctatccgata agtatagctt ttatcagact tttcgttttt agctgttcaa
     8581 atcagaagtc gtattccccg gtagaacaat attactggca gcaagttcgc ccatgttgtt
     8641 gtatatcgca caagcagctt cgatgatgga catcgccaga gctgcaccac tcccctcacc
     8701 taaacgcata tccatattga gataaggctc cagccccaaa tgcgagagcg ctatacgcgc
     8761 gcctttttct gccgacaagt gagaaggaat gagatacggt ttgattgcag gagacatctg
     8821 gcaggctgcg agcgcagcag cataagaaag aaatccatcc agcaacacgg gtaaaccgca
     8881 ggaagcagca cctaacatca ctccagctat tccgaccaaa tcaaatccac ccacttttgc
     8941 caggacatca acaccatcct gaggatttgg ttgattcaac gtaatcgccc gacgcacaac
     9001 atcaatttta ttagccagtt tatctgtcgg caggtttgcg ccaatcccaa ccacttcttc
     9061 aggatcccgg ccagtgattg tgctgactat tgccgctgcc ggtgtcgtgt ttgccatccc
     9121 cagttcacct acaccaaaca gcgtgacacc gtttttcgcc agctcccgcg tataacatat
     9181 gacgtccaaa agcaactttt cagcctgacg gcgactcatt gccggagctg aagcaatatt
     9241 gccgctacct cgtgcgacac gcatgttgat aagcccgggg ataggctcag cagtatcaat
     9301 accaacatca attacgtgga cgttagcgcc cgcttgtgct gccagcacac acacgccggt
     9361 tgttccacgg gtcatatttt cagcctgtat ggctgtcact tcttttggag aaatagcgac
     9421 cccttcctcc cagacgccgt gatcggcaca cataaccagt accgcttttt tgcccacatg
     9481 cggtatgcca ttcacccccg gcattcctgc cagttgtatg gcaagcgcct ccagctttcc
     9541 taggctacca acaggtttga ataacccgtc aacatgccgt tgtgcacgcg acatagcggc
     9601 agaattgatg gctgggatcg tattcagtaa atcggcaatt atttgcatgt cacgtttctt
     9661 tatagcagtg ccagcaggaa gaccagttca ccaagctcta tggctgctcc cagcgtatcg
     9721 cccgtttgcc cgcccagcgt acgtttgaga agctgaccga ggatgaaaat cgccaccatc
     9781 gtcaccacca tagcagccac accatgcata cccagcaata atacggcagc aaaaatagcg
     9841 gctaagccga gtgtgacgca ggtttgtcgc ccatcaattt tgccaatgaa cacattgcca
     9901 agcccttctt cccgcgcata acgatgacga tacatcaata atgcggcagt cccgcggctg
     9961 accgcacatg ctgccgccag cgatgcaagg atcggttcgc cacgcagagc caactcgctt
    10021 aacaccagaa tctttgccag taccacaaaa atcaatgcca gaccgccgtg ggtgcctaaa
    10081 cggctatcac gcatgatttc caacatgcga tcgcgactac gtgcagaaaa tacgccatca
    10141 caggtatcag cgaggccgtc cagatggaat tccccggtca tcagcgccag cacaagtacg
    10201 ctaaacagtg ccgccagtgg tgcgccacac catgcctgca gcaccatgaa gaccagcccg
    10261 ctaatcgcgc caagcaataa tccaatcaaa ggaaaagtaa taataccgcg agaataatgt
    10321 tcgaaatcca gtccctggga ccagcg
//
LOCUS       DAJTRN010000124         9774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRN010000124 DAJTRN010000000
VERSION     DAJTRN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            123..254
                     /locus_tag="OO328_003447"
     CDS             123..254
                     /locus_tag="OO328_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5931881.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OO328_003448"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OO328_003448"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5931882.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OO328_003449"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OO328_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OO328_003450"
     CDS             2687..3739
                     /locus_tag="OO328_003450"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5931883.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OO328_003451"
     CDS             3815..5248
                     /locus_tag="OO328_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5931884.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OO328_003452"
     CDS             5431..7611
                     /locus_tag="OO328_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5931885.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OO328_003453"
     CDS             complement(7752..9035)
                     /locus_tag="OO328_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5931886.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..>9774)
                     /locus_tag="OO328_003454"
     CDS             complement(9170..>9774)
                     /locus_tag="OO328_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5931887.1"
                     /translation="EGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLR
                     LAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKET
                     LDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSAR
                     LYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2387 a         2286 c         2479 g         2622 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcag
//
LOCUS       DAJTRN010000125         9626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRN010000125 DAJTRN010000000
VERSION     DAJTRN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            385..2016
                     /gene="oppA"
                     /locus_tag="OO328_003455"
     CDS             385..2016
                     /gene="oppA"
                     /locus_tag="OO328_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5931888.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2102..3022
                     /gene="oppB"
                     /locus_tag="OO328_003456"
     CDS             2102..3022
                     /gene="oppB"
                     /locus_tag="OO328_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5931889.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3037..3945
                     /gene="oppC"
                     /locus_tag="OO328_003457"
     CDS             3037..3945
                     /gene="oppC"
                     /locus_tag="OO328_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5931890.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3957..4970
                     /gene="oppD"
                     /locus_tag="OO328_003458"
     CDS             3957..4970
                     /gene="oppD"
                     /locus_tag="OO328_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5931891.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4967..5971
                     /gene="oppF"
                     /locus_tag="OO328_003459"
     CDS             4967..5971
                     /gene="oppF"
                     /locus_tag="OO328_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5931892.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6024..6353)
                     /locus_tag="OO328_003460"
     CDS             complement(6024..6353)
                     /locus_tag="OO328_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5931893.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6388..7848)
                     /gene="cls"
                     /locus_tag="OO328_003461"
     CDS             complement(6388..7848)
                     /gene="cls"
                     /locus_tag="OO328_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5931894.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7991..8164
                     /locus_tag="OO328_003462"
     CDS             7991..8164
                     /locus_tag="OO328_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5931895.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8219..9472)
                     /gene="kch"
                     /locus_tag="OO328_003463"
     CDS             complement(8219..9472)
                     /gene="kch"
                     /locus_tag="OO328_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5931896.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2511 a         2260 c         2402 g         2453 t
ORIGIN      
        1 tgtttgttaa ggcatggatg caagctatag attctgatac ggtcaataaa agagaatcgc
       61 ttaacaattt tgcaaaatgt attggcgagt aagaaccgca tttggtactt tccgggcaac
      121 cgccagacga ttctttattg gtaatgagaa taattaacaa ttaaagagcg tcgcgaaaga
      181 ataatgtgtc tcgacagggg agacacagta cgaatcgaca taaggtgatc gtctgaatca
      241 ccagaataaa taaagtcggt gatagtaata cgtaacgata aagtaacctg acagcagaaa
      301 gtctccgagc ttgtgcaggg tcccaatccg ggattacaca tgctggttaa taccagtaat
      361 tataatgagg gagtccaaaa aacaatgacc aacatcacca agagaagttt agtagcagct
      421 ggcgttctgg ctgcgctaat ggcagggaat gtcgcgctgg cagctgatgt acccgcaggc
      481 gtcacactgg cggaaaaaca aacactggta cgtaacaatg gttcagaagt tcagtcatta
      541 gatccgcaca aaattgaagg tgttccggag tctaatatca gccgagacct gtttgaaggc
      601 ttactggtca gcgatcttga cggtcatcca gcacctggcg tcgctgaatc ctgggataat
      661 aaagacgcga aagtctggac cttccatttg cgtaaagatg cgaaatggtc tgatggcacg
      721 ccagtcacag cacaagactt tgtgtatagc tggcaacgtt ctgttgatcc gaacactgct
      781 tctccgtatg ccagttatct gcaatatggg catatcgccg gtattgatga aattcttgaa
      841 gggaaaaaac cgattaccga tctcggcgtg aaagctattg atgatcacac attagaagtc
      901 accttaagtg aacccgttcc gtacttctat aaattacttg ttcacccatc aacttcaccg
      961 gtgccaaaag ccgctatcga gaaattcggc gaaaaatgga cccagcctgg taatatcgtc
     1021 accaacggtg cctatacctt aaaagattgg gtcgtaaacg aacgaatcgt tcttgaacgc
     1081 agcccgacct actggaacaa cgcgaaaacc gttattaacc aggtaaccta tttgcctatt
     1141 gcttctgaag ttaccgatgt caaccgctac cgtagtggtg aaatcgacat gacgtataac
     1201 aacatgccga tcgaattgtt ccagaagctg aaaaaagaga tcccggacga agttcacgtt
     1261 gatccatacc tgtgcactta ctattacgaa attaacaacc agaaaccgcc attcaacgat
     1321 gtgcgtgtgc gtaccgcact gaaactaggt atggaccgcg atatcattgt taataaagtg
     1381 aaagcgcagg gcgacatgcc cgcctatggt tatactccac cgtatactga tggcgcaaaa
     1441 ttgactcagc cagaatggtt tggctggagc caggaaaaac gtaacgaaga agcgaaaaaa
     1501 ctgctggctg aagcgggtta taccgcagac aaaccgttga ccatcaacct gcggtataac
     1561 acctccgatc tgcataaaaa gctggcgatt gctgcctctt cattgtggaa gaaaaacatt
     1621 ggtgtaaacg tcaaactggt taaccaggag tggaaaacgt tcctcgacac ccgtcaccag
     1681 ggcacttttg atgtggcccg tgcaggctgg tgtgcagact acaacgaacc aacttccttc
     1741 ctgaacacca tgctgtcgaa cagctcgatg aataccgcgc attataagag cccggccttt
     1801 gacagcatta tggcggaaac gctgaaagtg actgacgagg cgcagcgcac agctctgtac
     1861 actaaagcag aacaacagct ggataaggat tcggccattg ttcctgttta ttactacgtg
     1921 aatgcgcgtc tggtgaaacc gtgggttggt ggctataccg gcaaagatcc gctggataat
     1981 acctataccc ggaatatgta cattgtgaag cactaatggc aatacgtggg gcaggagtgt
     2041 cctgctccac ggtgtctgat ttttatcgca ttacagaagg cacaggccag aaggtagggc
     2101 aatgttaaaa tttattctac gtcgttgtct ggaagcgatt ccgacgctat ttattcttat
     2161 tactatttcg ttctttatga tgcgcctcgc accgggaagc ccttttaccg gcgaacgtac
     2221 tttaccgcca gaagtgatgg ccaatatcga agcgaaatat catcttaatg atccgatcat
     2281 gacacagtat ttcagctacc tgaaacaact ggcgcatggc gatttcggtc catcgtttaa
     2341 atataaagat tattcggtca atgacctggt ggcatccagt tttcccgttt ctgccaaact
     2401 gggagccgcc gcatttttcc ttgcggtaat actgggtgtt agtgctggcg ttattgccgc
     2461 attaaaacaa aacaccaaat gggactatac cgtgatgggg ctggcaatga ccggggttgt
     2521 tatccccagt tttgtggttg cgccattatt agtcatgata tttgcgatca ttttgcattg
     2581 gctgccgggc ggtggctgga atggtggggc gcttaaattc atgatattgc cgatggtggc
     2641 gttgtcactc gcctatatcg ccagtattgc gcgtattacc cgtggctcta tgattgaagt
     2701 attacactcc aactttattc gtactgcccg ggcgaaaggg ttacctatgc ggcggatcat
     2761 tttacgccat gcattaaaac ctgctctgtt acccgtgctc tcctatatgg gccccgcatt
     2821 tgtcggcatt attaccggtt ctatggtcat cgaaaccatt tatggtttgc cggggattgg
     2881 gcaactgttc gttaatggcg cattgaaccg tgactattcc ttagtgttaa gcctgaccat
     2941 cctggttggt gcgttaacca ttttgtttaa tgccattgtc gatgtgctat atgcggttat
     3001 cgacccgaaa atccgttact gatactggag ctcgcgatga tgttaagtaa gaaaaacagc
     3061 gagacgctgg aaaatttcag tgaaaagctg gaggtcgaag ggcgcagctt gtggcaggac
     3121 gcacgtcgac gttttatgca taaccgtgcg gcggttgcca gtctgatagt gctggtgctg
     3181 atcgcgttat ttgtaatcct ggcaccgatg ctttcccagt ttgcctatga cgatactgac
     3241 tgggcgatga tgtccagcgc cccggatatg gagtccggtc actactttgg tactgactca
     3301 tccggtcgcg acctgcttgt gcgcgttgcg attggcgggc gtatctcact catggtcggt
     3361 gttgctgcgg cactggtggc agtggtcgtg gggacacttt atggttcgct ttccggttat
     3421 ctgggcggta aagtggattc ggtaatgatg cgtctgctgg aaatcctcaa ctccttccca
     3481 ttcatgttct tcgtcatttt gctggtgacc tttttcggcc aaaacatcct gctgattttc
     3541 gtggcgattg gcatggtttc ctggctggat atggctcgta ttgtgcgtgg gcaaaccctg
     3601 agtctgaagc gcaaagagtt tattgaggcg gcacaagttg gcggtgtatc gacgccgggc
     3661 attgttattc gccacattgt gccgaacgta ctcggtgtgg tggtggtcta cgcatcgcta
     3721 ctggtgccca gcatgatcct ctttgaatct ttccttagct tcctggggtt gggtacgcaa
     3781 gagccgttaa gcagctgggg ggcattgctg agtgatggcg cgaactcgat ggaagtctct
     3841 ccatggttac tgttgttccc agcgggattc cttgtggtga cactgttttg tttcaacttt
     3901 atcggcgatg gcttgcgtga tgccctcgac ccgaaagatc gttaaggagt gcagccatga
     3961 gcgtaattga aactgcaact gtgccgctcg cacaacaaca ggctgacgca ctgctgaacg
     4021 tgaaagattt gcgtgtcacc tttagtaccc cggacggcga cgtcacggcg gtcaatgatt
     4081 taaatttttc cctacgtgcc ggagaaacgc tgggcattgt aggtgagtct ggttcgggta
     4141 aatcgcaaac tgcatttgcg ttgatgggcc tgttggctgc caacggacgc attggcggat
     4201 cggcaacctt caatgggcgt gaaatcctca atttgccaga gcgtgaactc aataaactgc
     4261 gcgctgaaca aatctcaatg atttttcagg acccaatgac ttcgttgaat ccctatatgc
     4321 gcgtcggtga gcagttgatg gaagtgctga tgctgcataa gaacatgagc aaagctgagg
     4381 cgtttgaaga gtcggtgcgg atgctcgatg cggtaaaaat gccggaagca cgtaaacgca
     4441 tgaaaatgta cccgcacgaa ttttctggcg gcatgcgtca gcgagtcatg attgcgatgg
     4501 cattgctatg tcgacctaag ttgctgattg cggatgaacc cactacagcg ctggacgtca
     4561 ccgtacaggc gcagatcatg acgctattga atgaactgaa gcgggaattt aatactgcca
     4621 tcattatgat cacccacgat cttggcgtgg tggcggggat ctgtgacaaa gtgctggtaa
     4681 tgtacgccgg gcgcacgatg gaatatggca atgcgcgcga tgtcttttat caacccgttc
     4741 atccttattc tatcggtttg ctcaacgcgg tgccgcgtct tgatgcggaa ggtgaaacaa
     4801 tgttgaccat cccgggtaat ccgccaaact tgctgcgatt accgaaaggt tgcccgttcc
     4861 agccacgttg tccacatgcg atggaaattt gtagtagcgc accgccgctg gaagagttta
     4921 cgcctggccg tctgcgtgct tgctttaaac cggtggagga actgttatga atgctgtaac
     4981 tgaaggaaga aaagtcctcc ttgaaatcgc cgatcttaaa gtgcactttg aaatcaaaga
     5041 tggcaaacag tggttctggc aaccgccgaa aacgctcaaa gccgtcgatg gtgtcactct
     5101 tcgcctgtat gaaggggaaa cattaggtgt ggtaggggaa tcgggatgcg gtaagtccac
     5161 ctttgctcgc gccatcatcg gtttggtcaa ggcgaccgac ggtcatgttg cctggttagg
     5221 taaagagttg ctgggcatga agcccgatga atggcgtgcc gttcgcagtg atattcagat
     5281 gattttccag gatccgttgg catcgctaaa cccgcgtatg accatcggcg agatcatcgc
     5341 tgaaccactg cgtacttatc atccgaaaat gtcacgccag gaagttcgcg agcgcgtgaa
     5401 ggcgatgatg ctgaaagtcg ggttattgcc taacctgatt aaccgctatc cgcatgagtt
     5461 ctccggtggg cagtgccagc gtatcgggat tgctcgtgct cttattcttg aaccgaagct
     5521 gattatctgc gatgagccgg tttcggcgct ggacgtgtca attcaggcgc aggtggttaa
     5581 cctgctccag cagctgcaac gtgagatggg attgtcatta atttttattg ctcatgacct
     5641 ggccgtggta aaacacattt ccgatcgtgt gttggtgatg tatctcggcc atgcagtaga
     5701 actggggacc tatgatgagg tctaccacaa tccactacat ccttacacca gggcattgat
     5761 gtcggcagtc cccatacctg atccggatct ggagaagaac aaaaccatcc agttactgga
     5821 aggggaatta ccgtcgccga tcaacccgcc ttccggttgt gttttccgta cccgttgccc
     5881 gattgccggt ccggagtgcg ccaaaacacg tcctgttctg gaggggagtt tcagacacgc
     5941 cgtttcttgc ctgaaagtcg atccgcttta aaaacaataa gggctgacag ttgtcagccc
     6001 tttttcacgc taaaagcgat tatttattcc cgccagatga tatggcaaag tttgtgatct
     6061 ttttcgcggc ataacagaat gcgggcgaaa acatcgctga tttcaccatc ttcactgtcc
     6121 gccagaccaa tcaccacttc ggcaaaaaag tccgggttca aatcgaaatc aacatgttcc
     6181 tgccagtctt ccgccggatc gaataactcc gcgccgccgc gctcttcaaa ctgaagattg
     6241 aacagcagaa catcagctgg atcgagattg tcggcagcca gttcgagaaa aatatcgtag
     6301 gcctgctcaa gcgtttcatc ttcagtcagg cgattgttta gatccatatc catagtcact
     6361 acctgtttaa cctctgttgg cgacgtttta cagcaacggg ctgaagaagt aaaacagtcg
     6421 ctcggcgaca cgttgccata atggacgttt tagccataaa cgggcatcga gcagacgtga
     6481 acgcgaaata taatcgtcct gaacggcggc aaggtctgca ccaaaacctt tatcgtcgat
     6541 tgccaaggta atctcgaaat tcagccacag actacgcata tcaaggttaa cggtgccaac
     6601 cagacttagt tcgccatcga ccagcacgct cttggtatgc agtaacccgc cttcaaactg
     6661 ataaatttta accccagcag caagcagttc cgtaaagaat gcgcgactgg cccagccgac
     6721 cagcatcgag tcattttttc gcggaaggat aatactgaca tccaccccgc gctgcgccgc
     6781 cgtgcaaatc gcatgaagta aatcatcgct tggcacaaag tagggcgtgg tcatgatcaa
     6841 atattcatgc gccgaataag ccgcagtcaa taatgcctgg tgaatgagat cttccggaaa
     6901 gccggggcca gaagcaattg tgtgaatggt gtgaccgctg gcctgttcaa acggcataat
     6961 attgacatct ggtggtggcg gcagaatacg ttttccggtt tcaatctccc agtcgcagga
     7021 ataaataatc cccatcgcgg tggcgatggg gccttccata cgcgccatca gatcaatcca
     7081 ttgccctacg cccgcatctt gtttgaagta gcgaggatcg accatattca tgctgccggt
     7141 gtacgcgatg taattatcga tcatgatcat cttgcgatgt tggcgcaggt ccatacggcg
     7201 taaaaacaca cgcatcagat tgacctttaa ggcttcgacc acttcaatac cggcattacg
     7261 cattagctcg ggccacgggc tgcggaaaaa agccacactc ccggcggagt cgagcatcaa
     7321 tcggcaatga ataccacgcc gtgcagccgc cattaatgat tcagccacct ggtccgccat
     7381 gccgccgggc tgccagatat aaaacaccat ctcaatatta tggcgcgcga gctggatgtc
     7441 gcggattaac gcctgcatca catcatctga ctcggtcatc agctgtagct gattcccttt
     7501 gaccccagcg atcccctgac gacgctcgca aagcttgaat aatggcgcag cgacactgct
     7561 attttcttcg gcgaagatat gcttacaggc tttaaggtcg ttaagccatt ttgcggtgga
     7621 aggccacatc gctctggcgc gctcagcgcg gcgtttgcct aaatggagct cgccaacggc
     7681 aagataggca ataattccga ctaacggcag aatgtaaata atcaacagcc aggccatcgc
     7741 ggagggaact gcgcgtcgtt tcattagaat gcgtaaagtt acgcctgcaa tgagcaacca
     7801 gtatcccaga atggccaacc aactcaccaa cgtataaacg gttgtcatag attagaaatc
     7861 cttttgaaag cgcattgtta tgagtttacg catatggatt catctggcaa ataaaaacgc
     7921 gggaaaagca ctggtcagcg tcgggggcgg gggtataatg accattctgt tattgcatag
     7981 agtagttaac atgaagcgga gtagaacgga agtggggcgc tggcgcatgc agcgtcaggc
     8041 tagccgacgt aaatcgcgtt ggcttgaggg gcaatcgcgc cgaaatatgc gtatccacag
     8101 catcaggaag tgcattctaa acaaacagcg taactcgtta ttgtttgcga tctacaatat
     8161 ctaaatgtac tgggcaccat tgcggtgccc aataagttta ctcttccgtc tttaaactct
     8221 atttttgcgc cgattcttta ctgtcagctt tcgtttcttg ttcatcgtta tcgctgaaaa
     8281 taccatgacc tgagttcaac aacattgaaa cgagcatatc attattaatc tcttcaccat
     8341 ttaaaactcg cgccagaatt tcgctgccaa acagttgcgg tgagagaatg atatccggat
     8401 gtaccatctt aatcttattc aggtttttgc tatcactgac ggcgagaact gttttgacat
     8461 cactgctcat atcttttgcc gagagtacaa caaacgcgtt atctgcatcg ttatcactca
     8521 gcgccagaat ggcccggcaa cgatcgattc ccgctttctt taatactgaa ctgtcattac
     8581 tgtcaccggg gataacatca gcgttatcgc ctaagcgttg ctcaagttgc ttgatatcat
     8641 cttcaggcaa gttgctgata accgttacgt tttgtccgcg ttgattcagt tgcaggatcg
     8701 tattgatggc gagaatcgaa tgtccgcaaa caataaaatg atctttacga tgcattgtat
     8761 gattgtttcc ttttaaaagt ttgttgaatc ccccgcggat aagcggacca aaaattgagg
     8821 tcatagatgt ggcaaaaacg gtaatgccgg aaataatgac cgaaatagtg aacaatcgtg
     8881 ctgattcaga aacagggaca atatctccat agccgacggt tgacatggtt tctatcgaaa
     8941 aatagaacgc ggtcatcaaa ctttctattc gcggattaaa accttcgctt aaataaagcg
     9001 caccgtaggt tgagtaaaac agtaatgtcg tgaaactaat aaatgcaaaa attgtcccgg
     9061 ctgcggcgct actgtgggag aagtctttgc gcagtatcag caaaaacacc agcgtaaaaa
     9121 tgcaaaatcc aatactaaat ttcagccagg gataaaaatg cagggtgtaa attagcgcta
     9181 tcaacaacaa aatgatactg attgcccatg cgagctttgc ccggaacagt aagccaatgg
     9241 agttcaggac aagaaagacg ccgagcataa atagtggggc gtttgccagc aaggagagat
     9301 ccaactctga aaatgctttg atatggaaaa tatcaagcaa attgaccgac ataccataga
     9361 ttgttttaaa aataagcaat ccatttaaaa agacggccag cgcgagaatg tcgtgccgta
     9421 gggtcaccca taaatttgtt gctgtttgtt tgaatgtagc ccagtgactc actgatacct
     9481 ctgtaaccac aattttgttt aattatctta gcgtgcacat tttctgaaca tacatgcagc
     9541 gcgtgaatgt gttaaacaat agtagatgac tttatccgca gtaatcaaag gttttgagcg
     9601 agcaactgta cctcaggaaa ggtcat
//
LOCUS       DAJTRN010000126         9601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRN010000126 DAJTRN010000000
VERSION     DAJTRN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(12..332)
                     /gene="psiF"
                     /locus_tag="OO328_003464"
     CDS             complement(12..332)
                     /gene="psiF"
                     /locus_tag="OO328_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5931897.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(451..1866)
                     /gene="phoA"
                     /locus_tag="OO328_003465"
     CDS             complement(451..1866)
                     /gene="phoA"
                     /locus_tag="OO328_003465"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5931898.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1967..2227)
                     /gene="iraP"
                     /locus_tag="OO328_003466"
     CDS             complement(1967..2227)
                     /gene="iraP"
                     /locus_tag="OO328_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5931899.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2409..2612)
                     /locus_tag="OO328_003467"
     CDS             complement(2409..2612)
                     /locus_tag="OO328_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931900.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2690..3784
                     /gene="ddlA"
                     /locus_tag="OO328_003468"
     CDS             2690..3784
                     /gene="ddlA"
                     /locus_tag="OO328_003468"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5931901.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3808..4020)
                     /locus_tag="OO328_003469"
     CDS             complement(3808..4020)
                     /locus_tag="OO328_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5931902.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4280..4588
                     /locus_tag="OO328_003470"
     CDS             4280..4588
                     /locus_tag="OO328_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5931903.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4647..5741)
                     /gene="yaiW"
                     /locus_tag="OO328_003471"
     CDS             complement(4647..5741)
                     /gene="yaiW"
                     /locus_tag="OO328_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5931904.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5754..6974)
                     /gene="sbmA"
                     /locus_tag="OO328_003472"
     CDS             complement(5754..6974)
                     /gene="sbmA"
                     /locus_tag="OO328_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5931905.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7326..8483
                     /gene="ampH"
                     /locus_tag="OO328_003473"
     CDS             7326..8483
                     /gene="ampH"
                     /locus_tag="OO328_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5931906.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8484..8987)
                     /locus_tag="OO328_003474"
     CDS             complement(8484..8987)
                     /locus_tag="OO328_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5931907.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9075..>9601)
                     /locus_tag="OO328_003475"
     CDS             complement(9075..>9601)
                     /locus_tag="OO328_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5931908.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2383 a         2450 c         2285 g         2483 t
ORIGIN      
        1 tcaatcaggt atcaggcggc tttcttgagg caggcactca taaacttatt acgatcatca
       61 cctttcagag cttgttgcgt tgcttgatta ttacattcac gcatcttttg ctgctgtggc
      121 gtcaaacttt tttcaccagg cgcagacttg ctgttcttca ggcaatcact catgtaggtc
      181 ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct gattacagga ggtcatacgc
      241 tgttgttgtg gggttaaggt tctctcggca gcgccgacgg tggttaaaaa aaccagacca
      301 aaaagcaagg taaccagtaa tgttattttc atagcaccat ccctcttcat gttttaacca
      361 tgagcgtttg cgcccgtgat ctgccattaa gtctggttgc taacagcaaa aaaaccaccc
      421 ggcagcgaaa attcactgcc gggcgcagtt ttatttcagc cccagagcgg ctttcatggt
      481 gtagaagaga tcggtctggt cggtcagtcc aacaacattg gcggcatgcg ggccatacgc
      541 cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcggaat tcccgtaact
      601 catcaccatc actgcgccat ctttagtatt tagcgcctga gtgaggcccg gcgctttggt
      661 atccggcgca acaacctggc tggcgtgggc gtgatcagcg gtgactatga ccagcgtgtt
      721 accctccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat cgaccgtctc
      781 gccaatttgc ccacaagggt tcgcagcgtg atcctgttta tcgattgacg caccttcaac
      841 ttgcaggaaa aatcctttct catttttact caacaattca atggctttgt cggtcatctg
      901 tgccagggtt ggtacactgt cattacgttg cggattaggc gtataggtga ctgcgggctt
      961 atcgatattg ccgtggtacg ttgctttcgg ccctagccag cgcactggca tattgccgtc
     1021 agcaaacagg ccaagcaggg gtttttgctg attcgcttcc gtcaccgaat tcagtgaggc
     1081 agcatcgttc accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg tttttccctg
     1141 ccattcacca gcggttgccg tttcagcaaa ggtttttgcg ccgccgccaa gcgtaacgtc
     1201 ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt ccagagcgtt
     1261 acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgaag tcacatgtgc
     1321 caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac cggtcgccag
     1381 acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa tatcgacgcc
     1441 cagcgcgccg ttataggttt tgacatcggt tgaccaggcg gttgctgatg cagccgagtc
     1501 ggtgacgtag tccggtttgc cagttttttt attcagcgca tagtgagtgt attgcccggt
     1561 aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat aattacgtgc
     1621 ggcagtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat tttttgcagg
     1681 tttatcgcta agagaatcac gcagagcggc ggtctgatca cccgttaagc ggcgagcacc
     1741 gccgggtgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca tttctggtgt
     1801 ccgggctttt gtcacagggg taaacagtaa cggtaagagt gccagtgcaa tagtgctttg
     1861 tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag cgactataag
     1921 tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac tgacaaggat
     1981 gcttcaataa cttctttacg taatcgcgca gcagctctgt atcgtcgtca ggaatgctgg
     2041 catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaaccgc tgttggtcat
     2101 tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct acctgcgcac
     2161 acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca gcaatgagat
     2221 ttttcatgtc tgtatttcct tatccaaagt atggagaagt taacattatg tttcacaact
     2281 tcatagtgtc tggaagtatt atttttatga caaaggaaat actttgcgca aatatttttt
     2341 gtttgattgc cagcgaaacg tttctcttat ttaaataatt acccacacaa aatataggct
     2401 tttaattatc aacctttatt ataaataaaa gctatgaaat atattctctt cgttaactta
     2461 taattaagag aaaaaaactc accgcattgc catttaagcc aggaataaat tagcttcccg
     2521 ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt caccagtacg
     2581 ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga atcccccgtt
     2641 ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga tggaaaaact
     2701 gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc
     2761 aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc tgggcattga
     2821 taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg cagacgatcc
     2881 tgcccatatt gcgttgcgcc cttcggcgac cagccttgcg caggtgccag gtaaacatga
     2941 gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg tcattttccc
     3001 gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa
     3061 tttaccgttt gtaggttctg atgttctggc ttcagcagcc tgtatggata aagatgtcac
     3121 caaacgtctg ctacgcgatg ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc
     3181 taatcgtcac aacatcagtt ttgccgaagt ggagtctaaa ctggggttac cactgtttgt
     3241 aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta
     3301 cgcaattgcc gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg agcaagggat
     3361 caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aatccgcaag ccagcacctg
     3421 tggcgagatc gtactcacca gcgatttcta tgcctacgac accaagtaca ttgacgaaga
     3481 tggcgcgaaa gtggtagttc cggcagccat tgcaccagag atcaacgata agatccgggc
     3541 gattgccgtt caggcttatc agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt
     3601 tttaacccca gagaacgaag tggtgatcaa cgagatcaac acactgcctg gcttcaccaa
     3661 catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac
     3721 acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat
     3781 gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg
     3841 acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata
     3901 ggtctgccaa ccttttgctt caaatcccag tacccccacc acgccattaa gaatgaatat
     3961 aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat
     4021 ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg tctgtggtgt
     4081 agacaacatt ttcgtaatta ttaataaatt taacttaagg ttgccatttt gtgactcaca
     4141 acgaagattc acgcttcggg aatattccca acatcaattt accatgattc tgatattgac
     4201 aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag
     4261 atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa
     4321 atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg
     4381 cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg
     4441 tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac
     4501 cattttcggg ctgatcccgt ttttagcagg ctgcctcatt tttgcagtgg tggcgctatg
     4561 gctgcactgg cgacatcgcc gccagtaaaa tttacgttgc cagatgcggc ctgaaacgcc
     4621 ttatctggcc tgacattcct gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct
     4681 acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga
     4741 atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc aagttgataa
     4801 actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc taactgacgg
     4861 cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt
     4921 ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg
     4981 ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta
     5041 aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag taaatggtaa
     5101 gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt accgtccatt
     5161 ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca
     5221 ccggtgcgca ccggattaag cgaaccaaac agcgtctgcc ccattggtac catgctgatc
     5281 agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta
     5341 taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa agcaggaatg
     5401 tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaacg
     5461 gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga gtagacgttt
     5521 tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca
     5581 tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac atctatcgct
     5641 ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa aaccagaacc
     5701 gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc tttttagctc
     5761 aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga
     5821 cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa
     5881 gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta
     5941 ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag
     6001 agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata gtttttccgt
     6061 acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca
     6121 taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc cagccccggc
     6181 agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca
     6241 atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc
     6301 gttaccagca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa actgacgccc
     6361 atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct
     6421 tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag
     6481 cggaacacgt agtgactgac aaagaagttg ttcagtacac tgatcaccac cgcgatcagc
     6541 gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc
     6601 gaacttagcg cggtttgaat cagatcatag aacggcgcat accaggcgtt gacggcgacc
     6661 ccgacttcca ccaaaaacca agtgacgaag ataatcagcg cagtaccgag tattgaccag
     6721 tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca
     6781 atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg
     6841 atctgcccgg aagcgccggt gatacgtgcc acccagtcac ccccaccggc ttgccagaag
     6901 ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa
     6961 aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt
     7021 aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga cccatagcaa
     7081 ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac aaaccaaaga
     7141 ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga
     7201 aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt
     7261 tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat ctatggacac
     7321 caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg
     7381 tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg
     7441 ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta
     7501 accagcgcgt ctttcgtagt tatggtgaaa cgcgacctgg taataacgtt cgcccgcagc
     7561 tggattcggt cgtgcgtatt gcttccctca ccaaactaat gaccagtgaa atgctggtga
     7621 aattgctcga ccaggggacc gtaaagttaa acgatccgct aagtaaatat gctccgccag
     7681 gcgcacgcgt gccgacctac aatgggacgc ccattaccct agtgaatctg gcaacccata
     7741 ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc
     7801 caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca
     7861 gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt
     7921 ccggcaagcc ttatacccag ttgtttgaag agcagattac ccatccattg gggatgaaag
     7981 acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg
     8041 ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg
     8101 gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc
     8161 aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa gtgattggca
     8221 tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag
     8281 agggtcgtcc gaggattatt cagaagacag gcggtggtgg cggtttcatt acctatatgg
     8341 cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc
     8401 gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac
     8461 cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt
     8521 ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg
     8581 acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc
     8641 aattcttcat tccagtcaat cattgataat aatgttgcgc gggtttgttt ggctggtaga
     8701 tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa
     8761 ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac gttttcttct
     8821 ctacgcagag agataactcc ttctaaaata acgaaagtat cttcgttatt gacatcactg
     8881 gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat
     8941 ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg cgcgtccttt
     9001 gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc agtatttctt
     9061 cggagtgaag aatattacca agtatattta acacccacgt tcgcggacca gtcttgatct
     9121 acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt ggtgaagctg
     9181 aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc gatggaatca
     9241 ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt gaagtacgga
     9301 gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc cagttcataa
     9361 cgcatgctgt cgtaaggctg accgtcaact ttcatgtcgt tgctcagctg gtagtcatca
     9421 ccagactgga acagaccaga aatgctgccg taaggagtca cgtaaccagc atcacccagt
     9481 ttgaagtcgt aaccggtttt caaaccgaag ccccaagcgt cggagttggt gctaccgtca
     9541 acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag
     9601 c
//
LOCUS       DAJTRN010000127         9519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRN010000127 DAJTRN010000000
VERSION     DAJTRN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..523)
                     /gene="pflA"
                     /locus_tag="OO328_003476"
     CDS             complement(<1..523)
                     /gene="pflA"
                     /locus_tag="OO328_003476"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5931909.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGW"
     gene            complement(715..2997)
                     /gene="pflB"
                     /locus_tag="OO328_003477"
     CDS             complement(715..2997)
                     /gene="pflB"
                     /locus_tag="OO328_003477"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5931910.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3052..3909)
                     /gene="focA"
                     /locus_tag="OO328_003478"
     CDS             complement(3052..3909)
                     /gene="focA"
                     /locus_tag="OO328_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5931911.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4316..6076)
                     /locus_tag="OO328_003479"
     CDS             complement(4316..6076)
                     /locus_tag="OO328_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5931912.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6206..6832
                     /locus_tag="OO328_003480"
                     /pseudo
     CDS             6206..6832
                     /locus_tag="OO328_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7031..8119
                     /gene="serC"
                     /locus_tag="OO328_003481"
     CDS             7031..8119
                     /gene="serC"
                     /locus_tag="OO328_003481"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5931913.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8190..9473
                     /gene="aroA"
                     /locus_tag="OO328_003482"
     CDS             8190..9473
                     /gene="aroA"
                     /locus_tag="OO328_003482"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5931914.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2419 a         2282 c         2393 g         2425 t
ORIGIN      
        1 accagcctgg gacaacaacg tagcggatcc acaccttcac atttttgttc gccagatatt
       61 tagcgaactc cagcgtgcgg tggttggaaa ctccaaccag attttggtgg atctcgtcgt
      121 tcatctgttt gagatcgagc attaccaggt cggttacttc cagcagttca tcaatcaccg
      181 gatcgtaacg acgaacaaaa ccgttggtat ccagacaggt atgaatgcct tcttttttac
      241 aggcgcggaa ccagtcacga acaaactcag cttgcaggat tgcttcaccg ccggatgcgg
      301 taacgccgcc gccggaagcg ttcataaagt ggcgataggt caccacttcc ttcatcaaat
      361 cttcaacggt aacttcttta ccgccatgcg tgtcccaggt gtcgcggtta tgacaataca
      421 ggcagcgcat caggcagccc tggaaaaagg tgataaagcg aatacctggg ccgtctacgg
      481 ttccacagga ttcaaaggag tgaatgcgac caataactga cattgcggtg tttctccaga
      541 tgtggcccat ctgaggccgt gttggtgcgc agctcgaagg ctacgtcgag tctgttttgg
      601 cagtcacctt aaagtataga tagctgacaa aaaaggctct cgtgctaaaa aaggccccac
      661 tttcgtggag cctttattgt acgcttttta ctgtacgatt tcagtcaaat ctaattacat
      721 agattgagtg aaggtacgag taataacgtc ctgctgctgt tctttagtca gcgagttgaa
      781 acgtactgcg tagccagata cacggatggt cagctgcgga tatttttccg ggttttccat
      841 cgcgtcgagc agcatttcac ggttcatcac gttaacgttc aggtgctgac caccttcgat
      901 ggatgcttcg tggtggaagt aaccatccat cagaccagcc aggttggtct tacgaacttc
      961 gtcgtcttta cccagtgcgt tcggaacgat agagaaggtg taggagatac catctttagc
     1021 gtaagcaaac ggcagtttag caacggaagt cagagacgct acagcacctt tctggtcacg
     1081 accgtgcatc gggttagcac ccggtccgaa cggcgcgcca gcacgacgac cgtctggggt
     1141 gttaccagtt ttcttaccat acacaacgtt agaagtgatg gtcagaacag actgagtcgg
     1201 gatagcgtca cggtaggtgt gcagtttctg aattttcttc atgaaacgtt ctaccaggtc
     1261 aacagccagg tcatctacac gcggatcatt gttaccaaac tgcgggtatt cgccttcgat
     1321 ttcgaagtcg atagccagac cgtcttcgtc acgaatcggt ttaactttcg catatttgat
     1381 tgcagacagg gagtcagcag caacggacag accagcgata ccacacgcca tggtgcggat
     1441 aacgtcacgg tcgtgcagcg ccatcagaga ggcttcgtag ctgtacttgt cgtgcatgta
     1501 gtggatgatg ttcagtgcag tgatgtactg tttagccagc cagtccatga agtgatccat
     1561 gcgctccatc acttcatcgt agctcaggag atcgcctttg atcggttcag acttcggacc
     1621 aacctgcatt ttcagttttt cgtcaacgcc gccgttgatt gcgtacagca tggttttcgc
     1681 caggtttgca cgcgcaccga agaactgcat ttgtttacca acgatcatcg ggcttacgca
     1741 gcaagcgata gcgtagtcat cgttgttgaa gtccggacgc atcaggtcat cgttctcata
     1801 ctgcagagaa gaggtgtcga tggacacttt agcggcgaat ttcttgaagt tcagcggcag
     1861 tttttcagac cacagaatgg tcatgttcgg ttccggagac ggccccatag tgtacagggt
     1921 gttcaggaaa cggaagctgt ttttggtaac cagggtacga ccatcgaggc ccataccacc
     1981 gatagattcg gttgcccaga ttgggtcgcc agagaacagt tcatcgtatt ccggagtacg
     2041 caggaagcga accatacgca gtttcatgac caggtggtca accatttcct gcgcttcttg
     2101 ttcggtgatc ttgccagctt tcaggtcacg ttcgatgtac acatccagga aggtggaggt
     2161 acgaccgaag gacattgcag caccgttctg agacttaaca gcagccaggt agccgaagta
     2221 agtccactgg atagcttcct gagcgttggt agccggacca gagatgtcgt agccgtattt
     2281 cgcagccatt tctttcatct gacccagagc gcggtgctgt tcagcgattt cttcgcgcag
     2341 acggatagtc tgttccaggt ttacgccgtt ttccagatca gcctgtagag aggtgaactg
     2401 agcgtatttg tctttcatca ggtagtcgat accgtacagc gcaacgcgac ggtagtcacc
     2461 gatgatacgg ccacggccat aagcatctgg cagaccggtc agaacaccgg atttacggca
     2521 acgcaggatg tccggagtgt aaacgtcgaa cacgccctgg ttgtgagttt tacggtattc
     2581 agtgaagatt tttttgatca tcgggtccag ttcgcggttg tacgctttgc aggaaccttc
     2641 gatcattttg ataccaccga acgggataag agcacgtttc agcggagctt cagtctgcag
     2701 accaacaact ttttccaacg ctttattgat gtagccagcg tcgtgagagg tgatggtgga
     2761 agcaacagcg gtgtcaaaat caactggcgc gtgagtgcgg ttttccagtt taacaccttc
     2821 cattactttg tcccacaggg tggtggtcgc ttcagtagcg ccagccagga aggactcgtc
     2881 accctcgtac ggagtgtagt ttttctgaat gaagtcacgg acgtttactt cattctgcca
     2941 gtcacctttg gtaaaacctt cccaggctgt ggctaacttt tcattaagct cggacatgta
     3001 acacctacct tcttaagtgg attttttatt tactgcgtac ttcgacaacc attaatggtg
     3061 atcgttttca cgcaggtaaa tgacccagta tgtcaaccca accaacaaac caccgccgat
     3121 aatattaccg atcgtaaccg ggatcaggtt atcagtgatg aagttcatca cggtcaggtg
     3181 agaaaaattt tccggtgcag aaccgacagc ggtccagaat tccggagatg cgaagtcgcg
     3241 gattacaata cccatcggga tcataaacat gtttgcgata ctgtgctcaa aaccgctggc
     3301 aacaaacatc gcgaccggca gcaccataat gaacgctttg tccatcaggc tgcggccaga
     3361 ataactcatc catactgcca gacataccat caggtttgcc aggataccaa gacagacggc
     3421 ctcaataaaa gtatggtgca ctttgtggtc ggcggtttgt aggacgttta gtccccattg
     3481 accatttgcg gtcatatact cgccggaaag ccacattaaa agtacaaaca gcagtgcgcc
     3541 gaccaggttg ccaaaataga catttagcca gtttttagcc aactgacacc aggtgatgcg
     3601 cccactcgcc ttagcaacaa caatcaacac tgtggaagta aagagatcgg ctccgcagac
     3661 aacacaaaga atcagcccca gagagaagca aatgccgcca accagttttg ccatgccgaa
     3721 gggcattgtg cctgtgccag tggttgctgt gatatagaag acgaatgcga ttgagatgaa
     3781 aacaccggcg gtaatcgcca gatagaaagt cttaagcgga tgtttcgttg ctttatagac
     3841 acccgcctct tcggccactt tggccattgc agcaggaagt aaaagatcaa aagggttgtc
     3901 agctttcaca ctaactctct ctttattaag tcggcgacga gatactaaca aagcattata
     3961 gatgagaaat tgatatagat catatctcgc ctggcttata ggcccgtaac tcgcatggtt
     4021 tttatgcaaa tacggagtaa ctatttgatt atccaaaata aaaataaatt ttaaaaatta
     4081 acaaatgagt tgaatttttt ccgcatcctc cgctaaaaca gttaattaaa agggagcatc
     4141 aggcgaataa agtaacaata tcgatcgtat ttattaaata caaattaccg atatttaacc
     4201 ttataattac aattatttta ttaatgcaaa tatatgtaaa gcggggcatt aaaaaaacgc
     4261 cccgtaatat aactcagact aatcattaag cctacattgc gtaggctatt tgattttatt
     4321 ttgcccagaa tgctgctttg gcgcgctgca gcttttcgta ggccttcaac aacgactgat
     4381 gtgcagcaaa cgcgtgcaga tcgctatcta ccggttgcag gccgtaaaac gccgcttcgc
     4441 cgctcattgc cgcactggcg gcttctacgg catctgcgcc gtacatgcga acaaacgcat
     4501 tcagatattg cagcggctgg cgatcttctt cctgtgccag taataacaac gtttgcaggc
     4561 agcgataata gttggcgcgt tccggactaa atactgatga gttaaactcc atcgtccatt
     4621 cggtccagac cagagcctgt tccagatcgc caccagccag cgccagcatg gcttttaatt
     4681 caccgatacg cagggtgtac caaccgttat ccgacccggt cgccagaccc aacagctcac
     4741 gcacgcgggt aaagtcatca aaaccttctt catccagttg ctcgatgagg ttcaggtaat
     4801 cttctttttc ccactcgctg cctggtagcg aaagaatcgt ttcacgtaaa tggctgccca
     4861 tactgttatt cgcgagccac agatcttcag ccggataaat atcggacatg ccaggcacga
     4921 taatacggca agcataaacg cccagatgct cgtaatcggc aatataaact tctttatctt
     4981 ctttgttgaa gatagccatc agcgtagcaa actcttcttc cgtggtgccg gagaaattcc
     5041 agtccacaaa cggataatcg gcatcctgct tgaacaggtc ccaggagatt aaaccgctgg
     5101 aatcgataaa gtgcgtttcg aggttggtat gttcagcgac ttcttcatca tcgaaggttg
     5161 gcggagtaaa cacatccaaa tctttcaggc cacgaccttg cagcagctcg gtcacggtac
     5221 gttccagtgc tacgccaaaa tcaggatgcg caccgaaaga ggcaaagcag gtgccgtttg
     5281 caggattaaa cagtaccacg caaatcaccg gatactggcc gccaagggaa ccatcatatg
     5341 cgaagatcgg gaaaccttct gtttccagtg tttcgatcgc ttcaaccacc gctgggtaac
     5401 gcgccagcac gtctgccggg atttccggca agctgatgct ttcagcaata atgcggtttt
     5461 tcacgtagcg ttcgaaaact tcggacaacc cctgaacgcg tgcttcgttg cgggtattgc
     5521 cagcggacat accgttagaa acgtacaggt taccaatgat attcatcgga atataaacgg
     5581 tctgattgtc ggactggcgc gtaaacggca gaccgcaaat accacgatct tcgttaccgg
     5641 attgtaggtc aatcagcata ctgccggtca attcattctc cggatcgtaa aatgcgcgca
     5701 gacggtcatc gagcagccct tctggcacat cgtcattttc gctcagtggg aaccattttt
     5761 cgttgggata atgcacaaat ggaccgttgg cgatggtttc gcccagccag aaatcagcaa
     5821 aaaagtagtt ggttgagaga cgctcgaaat attcaccgag tgcagaagcc agcgccgctt
     5881 tcttggttgc gcctttaccg ttggtaaaac acagtgcgca ctctttgtcg cgaatatgta
     5941 cagaccagac gttaggcacg ggattcagcc aggaggcctc ttcaatctga aagccgaggt
     6001 ctgaaagttt ttgctggaag cgagcgatgg aatcttccag agcggcatct ttgccgggga
     6061 taaatgtttg cgtcatgaaa atcactttag tcgtacggaa agcgcgcaat aatacgggtt
     6121 ttatctcaaa ggcgctatca ccgccgccat gccgatgaac tgttgactat gcttttagcg
     6181 gataaaccac gttaagagca taaaaatgaa ggcgttcgat ctccaccgta tggcatttga
     6241 taaagtgcct tttgatttcc ttggcgaagt tgcactacgt agtctttata cctttgtact
     6301 ggttttttgt tcctcaaaat gaccggaaga cgcggtgtgc gacagatgtc gctgtttgaa
     6361 gtgttaatca ttctcacgct gggttcagcg gcgggagatg tggcgtttta tgatgatgtg
     6421 ccgatggtgc cggtacttat cgtctttatc actctggcgt tgttataccg cctggtaatg
     6481 tgatggcgag ctggcctggt cgaaactcaa taactccaac atgacggaat ttgagttctt
     6541 tatggagcta cgattgcgtg gcgtggagca gctggggcag gtacgtctgg cgattctcga
     6601 aaccaacggg caaatcagtg tctatttctt tgaagatgac aaggtgaaac cgggtttact
     6661 tattttaccc agtgattgta cgcagcgtta caaagtggtg ccggagtcgg cggactatgc
     6721 ctgcattcgt tgtagtgaaa tcattcatat gaacgcgggg gaaaaacaat tatgtccgcg
     6781 ctgtgcaaat ccagaatgga cgaaggcaag tcgggcaaaa cgggtgacct gacagtaaaa
     6841 acatcggctt tttgctaata atccgagaga ttcttttgtg tgatgcaagc cacatttttg
     6901 ccctcaacgg ttttactcat tgcgatgtgt gtcactgaat gataaaaccg atagccacag
     6961 gaataatgta ttacctgtgg tcgcaatcga ttgaccgcgg gttaatagca acgcaacgtg
     7021 gtgaggggaa atggctcaaa tcttcaattt tagttctggt ccggcaatgc taccggcaga
     7081 ggtgcttaaa caggctcaac aggaactgcg cgactggaac ggtcttggta cgtcggtgat
     7141 ggaagtgagt caccgtggca aagagttcat tcaggttgca gaggaagccg agaaggattt
     7201 tcgcgatctt cttaatgtcc cctccaacta caaggtatta ttctgccatg gcggtggtcg
     7261 cggtcagttt gctgcggtac cgctgaatat tctcggtgat aaaaccaccg cagattatgt
     7321 tgatgccggt tactgggcgg caagtgccat taaagaagcg aaaaaatact gcacgcctaa
     7381 tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg gttaagccaa tgcgtgaatg
     7441 gcaactctct gataatgcgg cttatatgca ttactgcccg aatgaaacca tcgacggtat
     7501 cgccatcgac gagacgccag acttcggcaa agatgtggtg gtcgccgccg acttctcttc
     7561 aaccattctt tcccgtccga ttgacgtcag ccgttatggt gtaatttacg ctggcgcgca
     7621 gaaaaatatc ggcccggctg gcctgacaat cgtcatcgtt cgtgaagatt tgctgggcaa
     7681 agcgaatatc gcgtgtccgt cgattctgga ttattccatc ctcaacgata acggctccat
     7741 gtttaacacg ccgccgacat ttgcctggta tctatctggt ctggtcttta aatggctgaa
     7801 agcgaacggc ggtgtagctg aaatggataa aatcaatcag caaaaagcag aactgctata
     7861 tggggtgatt gataacagcg atttctaccg caatgacgtg gcgaaagcta accgttcgcg
     7921 gatgaacgtg ccgttccagt tggcggacag tgcgcttgac aaattgttcc ttgaagagtc
     7981 ttttgctgca ggccttcatg cactgaaagg acaccgtgtg gtcggcggaa tgcgcgcgtc
     8041 tatttataat gccatgccgc tggaaggcgt taaagcgctg acagacttca tggttgagtt
     8101 cgaacgccgt cacggttaat gccgaaattt tgcttaatcc ccacagccag cctgtggggt
     8161 ttttatttct gttgtagaga gttgagttca tggaatccct gacgttacaa cccatcgctc
     8221 gtgtcgatgg cactattaat ctgcccggtt ccaagagcgt ttctaaccgc gctttattgc
     8281 ttgcggcatt agcacacggc aaaacagtat taaccaatct gctggatagc gacgacgtgc
     8341 gccatatgct gaatgcatta acagcgttag ggttaagcta tacgctttca gccgatcgta
     8401 cgcgttgcga aattatcggt aacggcggtc cattacacgc agaaggtgcc ctggagttgt
     8461 tcctcggtaa cgccggaacg gcaatgcgtc cgctggcggc agctctttgt ctggatagca
     8521 atgatattgt gctgaccggt gagccgcgta tgaaagagcg cccgattggt catctggtgg
     8581 atgcgctgcg cctgggcggg gcgaagatca cttacctgga acaagaaaat tatccgccgt
     8641 tgcgtttaca gggcggcttt actggcggca acgttgacgt tgatggctcc gtttccagcc
     8701 aattcctcac cgcactgtta atgactgcgc ctcttgcgcc ggaagatacg gtgattcgta
     8761 ttaaaggcga tctggtttct aaaccttata tcgacatcac actcaatctg atgaagacgt
     8821 ttggtgttga aattgaaaat cagcactatc aacaatttgt cgtaaaaggc gggcagtctt
     8881 atcagtctcc gggtacttat ttggtcgaag gcgatgcatc ttcggcttct tacttcctgg
     8941 cagcagcagc aatcaaaggc ggcactgtaa aagtgaccgg tattggacgt aacagtatgc
     9001 agggtgatat tcgctttgct gatgtgctgg aaaaaatggg cgcgaccatt tgctggggcg
     9061 atgattatat ttcctgcacg cgtggtgaac tgaacgctat tgatatggat atgaaccata
     9121 ttcctgatgc ggcgatgacc attgccacgg cggcgttatt tgcaaaaggc accaccacgc
     9181 tgcgcaatat ctataactgg cgtgttaaag agaccgatcg cctgtttgcg atggcaacag
     9241 aactgcgtaa agtcggcgcg gaagtggaag aggggcacga ttacattcgt atcactcctc
     9301 cggaaaaact gaactttgcc gagatcgcga catacaatga tcaccggatg gcgatgtgtt
     9361 tctcgctggt ggcgttgtca gatacaccag tgacgattct tgatcccaaa tgcacggcca
     9421 aaacatttcc ggattatttc gagcagctgg cgcggattag ccaggcagcc tgaatgaaca
     9481 acgggcaata aatagccaaa tctttcttta tcaaaacgt
//
LOCUS       DAJTRN010000128         9393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRN010000128 DAJTRN010000000
VERSION     DAJTRN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO328_003483"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO328_003483"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5931915.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OO328_003484"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OO328_003484"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5931916.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OO328_003485"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OO328_003485"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5931917.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OO328_003486"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OO328_003486"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5931918.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OO328_003487"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OO328_003487"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5931919.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="OO328_003488"
     CDS             5060..6193
                     /locus_tag="OO328_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5931920.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OO328_003489"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OO328_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5931921.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO328_003490"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO328_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5931922.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO328_003491"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO328_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5931923.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO328_003492"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO328_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5931924.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO328_003493"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO328_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5931925.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="OO328_003494"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="OO328_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5931926.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2372 a         2421 c         2416 g         2184 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgcgc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agt
//
LOCUS       DAJTRN010000129         9374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRN010000129 DAJTRN010000000
VERSION     DAJTRN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..679
                     /gene="cmoA"
                     /locus_tag="OO328_003495"
     CDS             <1..679
                     /gene="cmoA"
                     /locus_tag="OO328_003495"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5931927.1"
                     /translation="RVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPGTQVYDLGCS
                     LGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRDIAIE
                     NASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGALVLSEKFSFEDAKVGELLFNMHH
                     DFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVA
                     LKAEDAA"
     gene            676..1647
                     /gene="cmoB"
                     /locus_tag="OO328_003496"
     CDS             676..1647
                     /gene="cmoB"
                     /locus_tag="OO328_003496"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5931928.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(1812..4241)
                     /gene="torZ"
                     /locus_tag="OO328_003497"
     CDS             complement(1812..4241)
                     /gene="torZ"
                     /locus_tag="OO328_003497"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5931929.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(4266..5366)
                     /locus_tag="OO328_003498"
     CDS             complement(4266..5366)
                     /locus_tag="OO328_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5931930.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(5754..6500)
                     /gene="cutC"
                     /locus_tag="OO328_003499"
     CDS             complement(5754..6500)
                     /gene="cutC"
                     /locus_tag="OO328_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5931931.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(6514..7080)
                     /locus_tag="OO328_003500"
     CDS             complement(6514..7080)
                     /locus_tag="OO328_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5931932.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            7296..9029
                     /gene="argS"
                     /locus_tag="OO328_003501"
     CDS             7296..9029
                     /gene="argS"
                     /locus_tag="OO328_003501"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5931933.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            9206..>9374
                     /locus_tag="OO328_003502"
     CDS             9206..>9374
                     /locus_tag="OO328_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5931934.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQE"
BASE COUNT         2275 a         2432 c         2271 g         2396 t
ORIGIN      
        1 acgggtcgct gaagtcttcc cggatatgat ccagcgttcc gttcccggct attccaatat
       61 tatttccatg attggtatgt tagccgagcg cttcgttcaa cctggtacgc aggtttacga
      121 tctgggttgt tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc atcatgataa
      181 ttgcaaaatt attgccatcg acaactcccc ggcgatgatt gaacgctgcc gtcgtcatat
      241 tgacgcctat aaagccccta cgccagtaga cgtcattgaa ggtgatattc gcgatatcgc
      301 cattgaaaac gcatcgatgg tggtgctgaa ttttaccctg caattcctgg aaccttccga
      361 gcgccaggca ttactggata aaatttatca aggactgaac ccgggcggcg cgctggtgct
      421 ttcggaaaaa ttcagtttcg aagatgccaa agttggtgaa ctgttgttca acatgcacca
      481 cgactttaaa cgtgccaacg gttacagcga actggagatc agccagaagc gcagcatgct
      541 ggaaaacgtg atgctgaccg attccgtgga aacccataaa gcacgcctgc ataaagccgg
      601 ttttgagcat agcgagctgt ggttccagtg ctttaacttt ggttcactgg tggcattaaa
      661 agcagaggac gctgcatgat cgactttggt aacttttatt ctctgattgc caaaaatcat
      721 ctttcacact ggctcgaaac gctgcccgcg cagattgcta actggcagcg cgagcagcag
      781 cacgggctgt ttaagcagtg gtccaacacg gtggaatttc tgcctgaaat taaaccgtat
      841 cgtctggatt tattgcatag cgtaaccgcc gaaagcgaag agccactgag caccgggcaa
      901 attaaacgca ttgaaacgct tatgcggaac ctgatgccat ggcgcaaagg gccgttctca
      961 ctgtatggcg tcaacatcga taccgaatgg cgttccgact ggaaatggga tcgtgttctg
     1021 ccccatcttt ctgatttaac cggacgcacc attctggatg tcggctgcgg cagcggttat
     1081 cacatgtggc gcatgattgg cgcaggcgcg cacctcgcgg tggggatcga ccctacgcaa
     1141 ctgtttctgt gccagtttga agcggtgcgt aaactactgg gtaacgatca gcgcgcacat
     1201 ttgttaccgt taggtattga acagcttccg gcactgaaag cctttgatac cgtcttttcg
     1261 atgggcgtgc tttaccatcg ccgttcgccg ctggagcatc tctggcagtt gaaagatcaa
     1321 ctggtgaatg agggtgaact ggtgctggaa acgctggtta ttgatggcga tgagaacacg
     1381 gtgctggtac caggcgatcg ttacgcgcaa atgcgtaatg tctatttcat cccttccgcg
     1441 ctggcgctga aaaactggct gaagaagtgt ggttttgtag atatccgcat tgtggatgtg
     1501 tgcgttacca ctactgaaga gcagcgacgc actgaatgga tggttaccga atctctgtcc
     1561 gatttcctcg acccacatga tccgagtaag acagttgaag gttatcctgc gcctaaacgc
     1621 gcggtgctga ttgcgcgcaa gccgtaaagg tctggtatta ctgccggatg cggcgtgaac
     1681 gccttatccg gcctacaaac ccttgctaat tcaatctatt gcagggacta tgtaggcctg
     1741 ataagcatag cgcatcaggc agttttacgt ttgcataacc tcagcgcccg tttccgggcg
     1801 ctattcactt cttacgcctg aacagcaggc ttatcaaatg ccgttaactt cggtgcgtta
     1861 cccgtatatt tttcgatata caccagcgca gagttaccgg cacaggcatt tgcccgctgc
     1921 gagctgggga tatccgccgt taacacgttc gcactgccgt ttttacacaa gccgttttcc
     1981 agatctggcc atgcaccttc atgcacgcat accacgcctt ttttgatccc gtcagtcacc
     2041 accgcgcctg tcaaaatctg accacgtttg ttccagacac gcaccagatc gccattcgca
     2101 ataccaaatc gagcagcatc ttcggtgtga atagtgatcg gttcacgatc ggcaaccgca
     2161 tattttttac gcagttccgc atagttaagc tgactgtgta aacggtgtgc cggatgtgcg
     2221 gtcagaagct gcaactgctt ctcgtcggcg gtacccttcc actcatcagg cgcaagccag
     2281 gttgggtgtg ccgggcaatc cttatagcca aatttttcca gcgttttgga gtaaatctca
     2341 attttgccgc ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa atcaccataa
     2401 cgaacgtact gttcgttctt ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag
     2461 aaggcattaa acattggcat agtgacgcgt tgcgcacggg cccctttctg agcggcatca
     2521 tagaaaaatt tcagccacgc catttcatct ttaccttcgg tatagatctc ttttccgcca
     2581 ggtttgagta attccgcaag atcggcaaac acgtcaaaat cgttacgcgc ttcaaattgc
     2641 ggagcaacag cctgcttcat cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc
     2701 aagtcattgc gctcaaacga tgtggtgatc ggtaatacga tatctgcgtg tttggctgct
     2761 gcggtccagt agcattcaga aacgacgatc atctccggtt tctgccaggc tttgatcaga
     2821 cgattagtat cctggtgatg ggtaaagtta ccgccacccg cccaccagat cattttgata
     2881 ttcggatacg tttgttcttt accgttatgc tgatatttgc cgccaggatt ttccagcgca
     2941 tcgacgatac gggcaacagg aatcgccgtc attccgccat catcagcggc ctcgctggcc
     3001 tgtccggcaa tcgccgcaga catttccggc agcacgccgc caacacgcgt cgggttgcca
     3061 ccattggagt aatgataaga gaaaccaaaa ccgccgcccg gtgtaccaat ctgccctaac
     3121 atcgccgcca gcgtcaccag catccagtgt ttctgttcac catattgctg gcgctgaatt
     3181 ccccagcctg ccatcagcat tgtgcgattc gccgccatca gttcagcgag tttgactatc
     3241 tgcgcttccg gtacgccagt aatttccgcc gtccagaccg cgctcttcgg cgtattatcg
     3301 ctcttacctg tcagatactc ttcaaactgc ggatacccgg tagtgtattt ttcaaggaac
     3361 actttatcgt gtttgccttg tgtcatcagg gtatgcgcaa tccctaacat cagtgccacg
     3421 tcggtgccca tattcggcgc gatccaggtg gcattatcgc caaagaattc gatggtttcg
     3481 gagcggatag gatcaatggc aatcactggt ttgccagatt ttttcagctg atggaagtat
     3541 tccagccctt gctcatcggt actgctccag gcaattttta aggtattcag cgggttcatt
     3601 ccccacagca ccacaacctg gctgttttcc agaatcagcg gccaggaggc ctgctgttca
     3661 tacacctcta cagaaccgac cacatgcggc atgatcacct gtgctgcccc ggttgaataa
     3721 tcgccgctat gcccggaata accgcccgcc aggttcatat aacgttgcag taaggtttgc
     3781 gctttatgca acacgccaga agagcgccag ccgtaagatc cggcaaaaat ggccgatggt
     3841 ccgttagctt tacgaatacg ctcatgttgc tcatgaatca gttttaatgc ctgttcccag
     3901 ctcacctgta cataggtatc ttcgccacga cctttcgccg gttgcagtgg attgtcgaga
     3961 tagctttttc tcaccatcgg atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc
     4021 gtagactgta aggagttcgg tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg
     4081 ccgtccttca cttctacgct catcgctccc caacgtcccg cggtgaggat tttaccgccc
     4141 ttctcttctg cccatgcggg gagcggtgct gccgatgtca ccaccagcgc tccagcggca
     4201 ataccgctgt gtttaataaa ttcacgtctt gttaatgcca taacttcctc cctgatcaac
     4261 gaggatcact gtttctcggt aatatctttg gcgttgtact ggaaataccg cgttaaaatg
     4321 tccggttcgt tttcgctcat gctggttcgt gcccccattc ctttggcaat ggacggccac
     4381 gcattgacgg tgtaatggtc ggcggcaata gaggcatgac aaccagcgca ataggtatcg
     4441 tcaagttttt cagcgtattg ccatagcggt ttacggtccg ctaatgcggg atcggtaagc
     4501 gcaccctgta aagacgcctg acgccattga ttgccgtatt cgtcagcctg ccattccccg
     4561 tttaccgtga gcgccttgat accttcttca cttaatgtgg ctagcgccag ccgttgacct
     4621 gccgccaggt agagcgtgtt ttcactgccc tgcatttgat aaccctgcaa cagaacgatc
     4681 ggctgtttgc cactggcatc aacgacggtg agatcggtac caggattcac ggtagccagc
     4741 tcgcctatgc gagaagtttt gaaaggataa atatgtgcgc cattagtcac tgaagtagcg
     4801 gcctgacttt ccagctcatg cgccgcgttg tcatccattt ttatttctgg cggaaaatgg
     4861 gcaatgcctt tatgacaatc gatacaggtt tcgccgccct tttgtgcttt gttatgcatt
     4921 ttctgcgcag attcactttg cgaggcaata tccatggcat caaaagaatg gcaactacgg
     4981 cacgttgcag agtcagttgc ttttaattct ttccatactg tttcggccat ttcctggcga
     5041 tgagtttcga acttatcgtc actgtctatt ttgccgctaa caaattcatg ataaatatct
     5101 ttagatgctt ttaatttagc aaataaataa tccatccctg actttggaat atggcaatcg
     5161 gcacattccg cacgtatccc tttctggttc gaaaagtgga cagttccctg atattcctca
     5221 aaaggtttac tcatcgagtg gcaggaaagg caaaatgctg tatccgacgt tttatgtaag
     5281 gctttttgcg ccagcaataa cccgccgcca ccaaccacaa ccgctatcag caaaaacaat
     5341 aacccaatgc gttttttccc tcgcatagat tcctctaaat aaattccaga acaccgaaat
     5401 cattgtataa ttaaatacac aacgaattaa ccgcttgcat aactcggcac aacactgcct
     5461 gaaataatcg ataaagaata tgatttatta caatgtaatc attaattgct aaggaataac
     5521 ccagttgcta tttagaatat atatttatca gttttagcaa tttaaatccc ataatcaatg
     5581 tgaatataaa aaatattttt agcacaacgc tatgcgcact gtcacccgct caacagatca
     5641 cggttatcgt tcgtttttta tactgctcag ggataaaaaa aggcccctgt tgaaattgca
     5701 ggggcctggt acgagcaagc atcatattgg gcgacatgat gcaacggtaa aaatcatttg
     5761 gcctgatggc gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc tacgatataa
     5821 cgcgaatact cgtccgcgtg ttcatctgat gacatggaca atccttgatt acgataacgc
     5881 atcggtgagg cttgccacgc tcccgcggag ctatggactt ccagcactcc ggcatcgagg
     5941 aagtggtgca ggttttctgc acggactcct gctccagcca taatgattgg agcatcacga
     6001 tgggcaataa gttccataat ttttgataaa ccttgcagtg cgtctgattt ttgccctgat
     6061 gtcagtaccc gggcaatgcc caattccgca agattattga gtgtatataa agggttagcg
     6121 cacatatcga aggcgcgatg aaaagtcact gccagcggac cggcagcagc cattattttt
     6181 tccattcgtg gcatatcgac attcccgtca acatcgagaa cgcccgtcac cagtccagga
     6241 aaacctaatt cgcggaccgt gcgcacatcc tcaagaatgg cggcaaactc accgtcgctg
     6301 tagcaaaaat caccaccgcg tgggcgaatt atcggatgca cagggatcgt cacccgctgg
     6361 cgcacggatt tcagtacccc caacgacggc gttaagcccc cctcttttgg ggctgcgcat
     6421 aattcaactc tgtctgcgcc gttttgctgc gccgttagtg cacattccat gctgtaacag
     6481 caaatttcca gtaatgccat ttttgctcct taattacgct gactgctcgc tggcaacgat
     6541 ctcttcaatg gaccacggat gaaacttaat agttgttttg ccatcggtga ccgccagcgt
     6601 cggattcggt aatcgctcat gctcaccttt tggtgaactg gttttaactg agatccccgg
     6661 taggctgagt ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg tttctggatc
     6721 acccggcaga acgatttcaa tatgttccca accttcgtgt gggtaacgtt tttccccggg
     6781 ccacggtagc tccacattgg aaaactgcca gtgcgcaacc tgtaccggtt catgcaattt
     6841 aaacagacaa atcggtctgc cattgatcat tttttctgac aaaagctcgc cacactgttc
     6901 aaaaccgcga cgccagcgct cagcggtgac gttttgatgg caacgcaaag aaatgtgatc
     6961 ggcagtcagc ggagtgatat ccaaccccag acggcgggaa agttcatcta atgcgtgggt
     7021 aaatcgcggt aaatccgatg caatatcctg cagctcgtcg atagattgcc agttcgccat
     7081 aatcactctt cgtctttcag taaaagcgtt aatttaccct gttgccctgt gccaaccaac
     7141 cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt
     7201 gctgacgaaa acagccattt gcagtatact cccgccctaa tttctttaac tggtgcgggc
     7261 aatttttgct cgcttcatca atgtaaggta ttccggtgaa tattcaggct cttctctcag
     7321 aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa ccgcagattc
     7381 gtcagtcagc aaaagttcag ttcggcgact atcaggctaa cggcatgatg gcagttgcta
     7441 aaaaactggg tatggcaccg cgacaattag cagagcaggt gctgactcat ctggatctta
     7501 acggtatcgc cagcaaagtt gagatcgccg gtccaggctt tatcaacatt ttccttgatc
     7561 cggcattcct ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc ggtgttgcta
     7621 cgccagaaaa acagaccatt gtggttgact actctgcgcc aaacgtggcg aaagagatgc
     7681 acgtcggtca cctgcgctct accattattg gtgacacagc agtgcgtact ctggagttcc
     7741 tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg gggcactcag ttcggtatgc
     7801 tgattgcatg gctggaaaag cagcagcagg aaaacgccgg tgaaatggag ctggctgacc
     7861 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     7921 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     7981 gcaaactggt cgacatcacc atgacgcaga accagatcac ttacgatcgt ctcaacgtga
     8041 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
     8101 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
     8161 ttgatgtgtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     8221 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     8281 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
     8341 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     8401 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtaccgtga
     8461 aactggcaga cctgctggat gaagccctgg agcgtgcacg tcgtctggtg gcagaaaaga
     8521 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
     8581 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     8641 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
     8701 ccgtgttccg taaagcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
     8761 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     8821 tggttgcccg tgaaggcacg ccgcatgtaa tgtgtgctta cctgtacgat ctggccggtc
     8881 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     8941 acagccgtct gaaactggca caactgacag cgaagacgct gaagctgggt ctggatacgc
     9001 tgggtattga gactgtagag cgtatgtaat cgatttttcg tgagagtgaa gcctgatcag
     9061 ggttagccga tcaggctttt ttattgccat ctaaatgtat tctgaaaacg gacatgccat
     9121 tgttttctca ctgttgagta agaggccaga agcgtaatat ccaggcccag gaaaacgata
     9181 acggttcaat ttaaggaata ccgaagtgtt taaatttctt gtattaacat taggcattat
     9241 ctcttgccag gcttacgcag aagatacggt tatagtaaac gaccatgaca cttcagccat
     9301 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
     9361 cctgcgacaa gaat
//
LOCUS       DAJTRN010000130         9341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRN010000130 DAJTRN010000000
VERSION     DAJTRN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(42..521)
                     /gene="xdhC"
                     /locus_tag="OO328_003503"
     CDS             complement(42..521)
                     /gene="xdhC"
                     /locus_tag="OO328_003503"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5931935.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(518..1396)
                     /gene="xdhB"
                     /locus_tag="OO328_003504"
     CDS             complement(518..1396)
                     /gene="xdhB"
                     /locus_tag="OO328_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5931936.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1407..3704)
                     /gene="xdhA"
                     /locus_tag="OO328_003505"
                     /pseudo
     CDS             complement(1407..3704)
                     /gene="xdhA"
                     /locus_tag="OO328_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4119..4874
                     /locus_tag="OO328_003506"
     CDS             4119..4874
                     /locus_tag="OO328_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5931937.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4953..5026
                     /locus_tag="OO328_003507"
     tRNA            4953..5026
                     /locus_tag="OO328_003507"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4985..4987,aa:Gly,seq:ccc)
     gene            5161..6771
                     /locus_tag="OO328_003508"
     CDS             5161..6771
                     /locus_tag="OO328_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5931938.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6803..7410
                     /locus_tag="OO328_003509"
                     /pseudo
     CDS             6803..7410
                     /locus_tag="OO328_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8341..9087
                     /locus_tag="OO328_003510"
     CDS             8341..9087
                     /locus_tag="OO328_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5931939.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2378 a         2307 c         2384 g         2272 t
ORIGIN      
        1 aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa
       61 tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt
      121 ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg
      181 gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga tttcgcataa
      241 gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct
      301 tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccaac
      361 accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt
      421 tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg
      481 ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg
      541 cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact
      601 ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag
      661 cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc
      721 gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat
      781 ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat
      841 atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga aaggcgacga
      901 ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga
      961 cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt agcgttggcg
     1021 tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac
     1081 ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga tgacgttgag
     1141 ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agcgagccat
     1201 cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat
     1261 aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg
     1321 cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag
     1381 aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat
     1441 aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg
     1501 ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt atgtccgtat
     1561 gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg
     1621 gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt agcatcgatg
     1681 atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct
     1741 tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat gcggttgatg
     1801 gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccggt
     1861 gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg ttcagggtgg
     1921 tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc
     1981 agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag catgactgcg
     2041 gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc aggcgcggca
     2101 acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc ggtatcttgt
     2161 gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag
     2221 aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca
     2281 tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag
     2341 gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg gttctggcat
     2401 tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac
     2461 cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg
     2521 cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac
     2581 tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg
     2641 ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact
     2701 ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac
     2761 agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat ttgcccgtca
     2821 atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact
     2881 ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc ttccagtaca
     2941 tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac gcatgaccag
     3001 ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa
     3061 acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct ttccatgtga
     3121 caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt
     3181 gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc gccgttatgg
     3241 attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg taattcttgc
     3301 cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg ggccacgacg
     3361 atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg atcggcggta
     3421 tcgcgcttgt tttcgtcaag tgtctaggca tgcccagctg tagcgaatgg aatatcaggc
     3481 acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg ttcatcatta
     3541 atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta acacatgccc
     3601 gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcgacg
     3661 cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt
     3721 tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac
     3781 gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac agatatgata
     3841 cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg ataaaatcaa
     3901 tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag tcaggaactt
     3961 cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat gaaattaacc
     4021 tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga ggctggctgc
     4081 tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga cgcctgaata
     4141 aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg ggctgttcgg
     4201 gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg aaacgggggg
     4261 atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg gcgcgactga
     4321 acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg ggtggggcga
     4381 aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc gcatcggtta
     4441 caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt tgttggttat
     4501 ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc aataaaggga
     4561 ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga aaggtggtgt
     4621 atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac agtgaagatt
     4681 acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa agcgtgaagg
     4741 ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc ctgcatttcc
     4801 agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg cagggcagca
     4861 agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg ctaaggagag
     4921 cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg tagaacgaga
     4981 gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac acttcttaaa
     5041 gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata gttttactca
     5101 ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag gtctcagtac
     5161 atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat ccgtcttagg
     5221 atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta taaactagcg
     5281 tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg gcacatcaag
     5341 tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga gctacgggag
     5401 cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct gaacgacccg
     5461 gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct tcgggaagct
     5521 gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt tatcggtgtg
     5581 cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc ttaccttgag
     5641 cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga acctgaagtc
     5701 cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca ggagaacgcc
     5761 cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac gctaatcgag
     5821 atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga tatgctcaga
     5881 gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa gtctatgccg
     5941 ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt taccattaac
     6001 aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa cgacttgata
     6061 gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc ctccgacgct
     6121 cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa agcgtactcg
     6181 cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc gattacaggt
     6241 gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac tgaggctggg
     6301 accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag tatcaagaac
     6361 gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt attggctgac
     6421 tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat ggtgttcaat
     6481 ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt taaccgaacg
     6541 ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt tagacataca
     6601 gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc gattatgggt
     6661 catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt ggaaacgctg
     6721 aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta atcgcatagg
     6781 gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag aaaataagcc
     6841 ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa tggcttcgga
     6901 agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt ttgctattga
     6961 ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga cgcagaaaga
     7021 gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca attatatggg
     7081 gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta ttgctcacgg
     7141 cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc aagacgatat
     7201 tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt tgagcgcctt
     7261 gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa tggttacagg
     7321 ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc ttttggcgtc
     7381 agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat agaggaatgg
     7441 acccttaact atcactatag gggagagacc ctaagtcccc accttaatag cagtcacgac
     7501 ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga aaccatccgc
     7561 agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta agctatcttc
     7621 ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc tgtgataatc
     7681 gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc catttgtgga
     7741 caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt tgagtcttga
     7801 ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat cacaattcca
     7861 ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa ttatggtcgc
     7921 tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt cgcgtcaagt
     7981 tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat ctcgcctctg
     8041 acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta gccaccagcc
     8101 taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact taagtctatc
     8161 accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc agaccactaa
     8221 ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc cctttacgtg
     8281 ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga gtacacgtaa
     8341 atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat gacctcagtt
     8401 gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat tcgtaatatg
     8461 gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag cgagcttaac
     8521 gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg tgagagccga
     8581 acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta ttccgttcac
     8641 ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct tcaactggaa
     8701 gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac ttggggcgac
     8761 tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct ccagcatcgt
     8821 ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa gtattccgac
     8881 tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta taacttccgg
     8941 ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa gatgcgaggc
     9001 atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct gaagctggca
     9061 caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac ctgaggggcg
     9121 catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc ttggtctaga
     9181 cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc aactggtcgc
     9241 tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt taaacacctt
     9301 ttgagatgac cgatgcgggg agacccgtag gccttactgt g
//
LOCUS       DAJTRN010000131         9267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRN010000131 DAJTRN010000000
VERSION     DAJTRN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            39..1154
                     /locus_tag="OO328_003511"
     CDS             39..1154
                     /locus_tag="OO328_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931940.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="OO328_003512"
     CDS             1833..2051
                     /locus_tag="OO328_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5931941.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="OO328_003513"
     CDS             2269..2511
                     /locus_tag="OO328_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931942.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO328_003514"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO328_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5931943.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO328_003515"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO328_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5931944.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO328_003516"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO328_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5931945.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO328_003517"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO328_003517"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5931946.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..>9267
                     /gene="fdhD"
                     /locus_tag="OO328_003518"
     CDS             8558..>9267
                     /gene="fdhD"
                     /locus_tag="OO328_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5931947.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEIL"
BASE COUNT         2269 a         2279 c         2403 g         2316 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtt
//
LOCUS       DAJTRN010000132         9265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRN010000132 DAJTRN010000000
VERSION     DAJTRN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..612)
                     /locus_tag="OO328_003519"
     CDS             complement(<1..612)
                     /locus_tag="OO328_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5931948.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRVMLPT"
     gene            complement(679..1404)
                     /gene="fimC"
                     /locus_tag="OO328_003520"
     CDS             complement(679..1404)
                     /gene="fimC"
                     /locus_tag="OO328_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5931949.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1441..1980)
                     /locus_tag="OO328_003521"
     CDS             complement(1441..1980)
                     /locus_tag="OO328_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5931950.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2045..2593)
                     /gene="fimA"
                     /locus_tag="OO328_003522"
     CDS             complement(2045..2593)
                     /gene="fimA"
                     /locus_tag="OO328_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5931951.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3074..3670)
                     /gene="fimE"
                     /locus_tag="OO328_003523"
     CDS             complement(3074..3670)
                     /gene="fimE"
                     /locus_tag="OO328_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5931952.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4148..4750)
                     /gene="fimB"
                     /locus_tag="OO328_003524"
                     /pseudo
     CDS             complement(4148..4750)
                     /gene="fimB"
                     /locus_tag="OO328_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6204..6920
                     /gene="nanC"
                     /locus_tag="OO328_003525"
     CDS             6204..6920
                     /gene="nanC"
                     /locus_tag="OO328_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5931953.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6940..8046
                     /gene="nanM"
                     /locus_tag="OO328_003526"
     CDS             6940..8046
                     /gene="nanM"
                     /locus_tag="OO328_003526"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5931954.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8111..9091
                     /locus_tag="OO328_003527"
     CDS             8111..9091
                     /locus_tag="OO328_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5931955.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2718 a         2019 c         1946 g         2582 t
ORIGIN      
        1 agttggcagc attacccgat tctgtacact atttccgctg aaattataat tgagcaatcc
       61 ggcattaata ccgggatccc ataactcagg aggaatataa ccacgcgcgc gattactcat
      121 aaatgcctga gggatcgtca ggttcagtcg ctgctgacca acatccagat gcgcagtagc
      181 gtcctggacc attgtggtta atggcacaca ggcatcatcc gccagcagat tcataccggc
      241 gacagaagcc gtattcagcc ccatactggc gagttgcgcg cgtgtcaggc agggaacaat
      301 cccttgttca ctgtcgcccg tattaaatgt gacatcacgc gttgccatat aaccattatt
      361 caaatagata tcgacgcgat acgtccctgg cggtaattct tgcccatttt caaaacgcga
      421 taaatcggcc acagcctggg gatcatccgc taaaaagcgc agattaaaat agaggtcggc
      481 agatgacaaa ggtgcctgtg cggcaaaagc acaggcgaca acgagtcgga caaaaaaacc
      541 agccaaacga tgcttacgaa tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt
      601 cagatatgac attattcctc cggtttaaaa tcacccggca gtcaattctt tttattcagg
      661 cgaaaaattc cccctgcgtt attccattac gcccgtcatt ttgggggtaa gtgcgccata
      721 atcatttatt gttcggtaag taatattgct tcctgcatca gaaggcaatt taaccgtgct
      781 ttcgcccatt ggaggcacca atgcattttc aagaacccgg gttccggcat tcaactctgt
      841 taccgtcagg taatagggtg tcgggttaat cagcgtcaga gaattcgcgc tacgacgaaa
      901 tcttaatttt tctgcggcct gatcgggtgg caacgctaat ttagccgggc gatagtacag
      961 tttaatgcgg ctgataattg cgagctgtag catattctca gtcaattttg atttatccat
     1021 tgacggaatc gctttaacgt tcatccagaa taaactttcc cggtcctgtg gcaattggtt
     1081 atttgttgca tcaagaatac gtaaggtatt ctcttttttt cccttcatcg caaacagagg
     1141 aggcgtcacg ataaaacgac catcctttac accatcggca ttttccaccc atgattgaat
     1201 taaataggta ctattttcat cattatttgt cacggcaagt tgcacttgtt tttgccctgc
     1261 cggataaatt acgcgagtcg cacctaaggc cactcctgct tcagcgcgtc cggcaaccat
     1321 cattgccatg aacatcagga tacctgccag caagcagaat gttatttcct gcgatttcct
     1381 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
     1441 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
     1501 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
     1561 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
     1621 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
     1681 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
     1741 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
     1801 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
     1861 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
     1921 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
     1981 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
     2041 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
     2101 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
     2161 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tctaggatct
     2221 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagccagt acatcagtac
     2281 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
     2341 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     2401 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     2461 cgcaagcggc gttaacaact tcccctttaa agtgaatggt cccaccattt accgtcgtag
     2521 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     2581 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     2641 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     2701 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     2761 cagtttattt gttggcttaa tattctatta ttatctttat ttatagattt ttatattgca
     2821 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     2881 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     2941 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat
     3001 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     3061 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     3121 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     3181 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     3241 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     3301 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     3361 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     3421 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     3481 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     3541 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     3601 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     3661 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     3721 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     3781 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     3841 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     3901 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     3961 tgattttgtt tatttatttt gaagaacata aatcattaga ttttgtatgg gtatggttaa
     4021 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     4081 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     4141 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     4201 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     4261 aatcctagat aagtcgcgta tctattccca tattcgccaa agcaaaacca cacgaatagc
     4321 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     4381 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     4441 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     4501 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatggata tatatacact
     4561 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     4621 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg
     4681 ctttaaggag tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct
     4741 tattcctcat cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
     4801 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg
     4861 taagtggacc agttcttctg agcgaactta aatggagtag cgatagttac ttataataac
     4921 tttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga
     4981 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
     5041 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttaaa
     5101 ctagccgtct caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca
     5161 tggaacgcag taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta
     5221 ttcctgcctg gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga
     5281 tatgggggaa cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc
     5341 actacgactt aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta
     5401 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag
     5461 tgaccagtta caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct
     5521 gatggcgagt aaacccttat tttcaccact ctcactgagt tagcttgcaa agctttcttt
     5581 gtttagattc taaataaatt atttataaaa cgtatagtta aaagaaaatc tttagttttt
     5641 gaagaccacc gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg
     5701 acatattttg tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga
     5761 tctggtataa caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat
     5821 ctatggcaaa catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga
     5881 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt
     5941 gagtaagtga tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac
     6001 tcaggtattt aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg
     6061 agaccttata caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg
     6121 caaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
     6181 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
     6241 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     6301 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     6361 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     6421 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
     6481 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     6541 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     6601 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     6661 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     6721 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     6781 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     6841 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     6901 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     6961 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     7021 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     7081 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     7141 gctgcattcc ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg
     7201 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     7261 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccgatgggc
     7321 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     7381 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     7441 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     7501 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     7561 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     7621 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     7681 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     7741 gggataagca atgattctct tatatttgct ggaggggccg gattcaaagg ttcacgagaa
     7801 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     7861 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     7921 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     7981 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     8041 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     8101 cgaaaattat atgaacgcaa taatatcacc cgattattac tatgttctta ccgttgctgg
     8161 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     8221 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     8281 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     8341 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     8401 gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc
     8461 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     8521 cggagccagc catgatgctt gtcgttgggg aacggatact ccgctgtacc aggatttagt
     8581 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     8641 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     8701 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     8761 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     8821 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     8881 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     8941 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     9001 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     9061 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     9121 tgttcgttct cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt
     9181 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     9241 caccgcataa accgttgtta ttgct
//
LOCUS       DAJTRN010000133         9232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRN010000133 DAJTRN010000000
VERSION     DAJTRN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO328_003528"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO328_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5931956.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO328_003529"
     CDS             1828..3147
                     /locus_tag="OO328_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5931957.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO328_003530"
     CDS             3445..3486
                     /locus_tag="OO328_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931958.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO328_003531"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO328_003531"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5931959.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO328_003532"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO328_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO328_003533"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO328_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO328_003534"
     CDS             5815..6711
                     /locus_tag="OO328_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5931960.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO328_003535"
     CDS             6708..7682
                     /locus_tag="OO328_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5931961.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO328_003536"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO328_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO328_003537"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO328_003537"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5931962.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2255 a         2255 c         2316 g         2406 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat at
//
LOCUS       DAJTRN010000134         9192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRN010000134 DAJTRN010000000
VERSION     DAJTRN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..904)
                     /locus_tag="OO328_003538"
     CDS             complement(<1..904)
                     /locus_tag="OO328_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5931963.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO328_003539"
     CDS             964..1242
                     /locus_tag="OO328_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5931964.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO328_003540"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO328_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5931965.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO328_003541"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO328_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5931966.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO328_003542"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO328_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5931967.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO328_003543"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO328_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5931968.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="OO328_003544"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="OO328_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="OO328_003545"
     CDS             complement(4922..5452)
                     /locus_tag="OO328_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5931969.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO328_003546"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO328_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5931970.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="OO328_003547"
                     /pseudo
     CDS             complement(6421..7644)
                     /locus_tag="OO328_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8225..>9192)
                     /locus_tag="OO328_003548"
     CDS             complement(8225..>9192)
                     /locus_tag="OO328_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5931971.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGSDNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2683 a         1826 c         1922 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttaccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgatcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc tg
//
LOCUS       DAJTRN010000135         9158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRN010000135 DAJTRN010000000
VERSION     DAJTRN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            328..480
                     /gene="hokE"
                     /locus_tag="OO328_003549"
     CDS             328..480
                     /gene="hokE"
                     /locus_tag="OO328_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5931972.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(602..1222)
                     /gene="entD"
                     /locus_tag="OO328_003550"
     CDS             complement(602..1222)
                     /gene="entD"
                     /locus_tag="OO328_003550"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5931973.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1397..3637)
                     /gene="fepA"
                     /locus_tag="OO328_003551"
     CDS             complement(1397..3637)
                     /gene="fepA"
                     /locus_tag="OO328_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5931974.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3880..5082
                     /gene="fes"
                     /locus_tag="OO328_003552"
     CDS             3880..5082
                     /gene="fes"
                     /locus_tag="OO328_003552"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5931975.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5085..5303
                     /gene="ybdZ"
                     /locus_tag="OO328_003553"
     CDS             5085..5303
                     /gene="ybdZ"
                     /locus_tag="OO328_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5931976.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5300..9145
                     /gene="entF"
                     /locus_tag="OO328_003554"
     CDS             5300..9145
                     /gene="entF"
                     /locus_tag="OO328_003554"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5931977.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         1989 a         2421 c         2527 g         2221 t
ORIGIN      
        1 gcatatccgg ttgtcaggtc aggatggtaa ggcacctgct ttacactttc gcccgcggtc
       61 agtgatggct gcgggcgatt tcgttttaag agcaatgatg agttttgccg acagaagtcc
      121 gaaacaggac tcactgtata aataaacagc tattttgcta aggaagggta agataacggc
      181 gggtgcctga agctttccgg tttcaggtta actctgaggt ttggaaagat gaagccccaa
      241 gagatatttc tatcaatcct ggggctgcca ctccgaacct caacaactcg gatggtagtc
      301 ccttcttcgc atggaggcaa tataaacatg ctgacgaaat atgcccttgt ggcagtcata
      361 gtgctgtgtt tgacggtgct gggatttacg cttctggtcg gagactcgct gtgtgagttc
      421 accgtaaagg aacgtaatat tgagtttaag gctgttcttg cttacgaacc gaagaagtag
      481 ccgttgtgcg gggagtaatc cccgcatatc cggttgtcag gtcagagtgg taaggcacct
      541 gctttacact ttcgcccgca gtcagtgatg gctgcgggcg aatcgtacca gatgttgtca
      601 attaatcgtg ttggcacaga gttatgacta ttttttcttt tatctgccag tgcacagcaa
      661 acatctcatt ctcacgatga atgatgacct gctgtttatt ccagctaatt atctgatagt
      721 ccagaaaacc tgcatcagtt tggatctcac ttgccttaaa tgcgctctct ttggcggaaa
      781 atgcaagtgt cagcgccaga gaaaaggcta aaccgcagtc tgcgagtcgc tcgtgttctg
      841 ctggtgtaat aatgttgtct gtcaattcgg tcgctgtttg cgcagaaaaa atttcttcga
      901 tatcaacgcc aatcggttga cgggaaacca cggccaatgc cgtagccccg cagtggctaa
      961 tactgccgta tacctccgca ggccacaccg gttggcgtag ctcgccgatt gcgggcacac
     1021 atttatagcc atattcccgc aaggcataaa cagcagcgat ccgtccggct aaatgctctg
     1081 ttttacgttt acgtccagcg tgttgcagtt gtgcgtagtg cggcagccag agtaaatcct
     1141 gctcacaaaa attcgccgga tcgaactcaa caaaatgcag cgtatgtccg gcaaagggga
     1201 gggaggtatg cgtagttttc atatcgacca tattcgagac tgatgacaaa cgcaaaactg
     1261 cctgatgcgc tacgcttatc aggcctacgt gatcctgcaa tatattgaat tggcaagatc
     1321 tttgtaggcc ggataaggcg ttcacgccgc atccggcgtg aacgacgcgc actttgtcaa
     1381 caatctgacg ttagcatcag aagtgagtat taatgctcat ataccacgta cgtcccggct
     1441 cgttataggt atacgcgccg gcaccggcga tatagttggc tcctgccaga tcgcccgtgg
     1501 tctgggcatt acctgcacgc cacaaacgtt tgtcgaacag gttgtccacg ccgccggtca
     1561 gactgacatt cttcgtcaca tcccaggtcg cgctcagacc cacaatgctg taaggactga
     1621 tttctttggt ttccgggcca accgctggct gacctttata gttgtacttc ttcggctgct
     1681 gcttgccgta ccaggtgaag gtcgtttgca tcgacaaatc ttcccgtgcc tgccagctca
     1741 gcgttgagtt caacgtgtac tccgggatga tcgacaaacg gtcgcccgtg gttttgtttt
     1801 cactcttcag catataagtg atgttattgg tccacatcac cgtttcgcta actggtacgt
     1861 ttaacgatcc ttccagacct tcaaccactg ctttcggcac gttatcccac tgatagagat
     1921 cagtgccgac tgcgttttgc cctacagcca catagcccgc ttcaatctta ttgcgataat
     1981 cgttacggaa ccaggtgacg cccgccagcc agccgtcgcg tttgaactcc agaccaatct
     2041 ctttgttgat gctggtttct actttcaggt catcgttacc ttgcagatag cagccgcccg
     2101 cgctggcata gcagccctga cctttactgt agagaatgta gttcgggtta gtctggtata
     2161 ggctcggcgc tttataggcg cgggcaatgc ctattttcag cgtgaagtca tcgcctaaac
     2221 cttgcgatat gttcagcgcc gggctccagt tattgccgac aatactgtga tgatcgaaac
     2281 gcagccccgg cgttacgatg gtgctgtcag tcagctccat gttgttttcg gcaaacagcg
     2341 agaaaatttc tgcttttgaa tacgggctac ggtcggtggc actcacgcca tcaatagcgc
     2401 caccggtatt cgttccggtc agtgcttggg tgttggaact taagtccttc atccgttgct
     2461 gattccactc cgtacccagc gtcagcgtct ggttaacgag gaaatcaatc ggcaggttaa
     2521 cttcgctgtg cagcatcacg tcatcgagat cgatatcgac gaaatcctgt gtcgcttttt
     2581 cgttaaattt cccttcggta ccgcccgcca gaccttccgg aatacgcgag ttacgggtgt
     2641 gttcatactg cacccagttg ctggtggtca cgccgttatc ccagccaccg ttccaggtca
     2701 gcgcgtagtt ctggcgatac aggcggttgg tttcatcgcc atatttcgag cgagtgtaag
     2761 cgtcagagtt ggtgttctgg gtatcgcccg catacaggtt accctggcgg ctgtaacccg
     2821 cttccagttc cagcgattgc aatggtgcga agtcccagcg caccacgccg ttaatatctt
     2881 tattgatcac cccttcgcgc ccggctggta acgtcgtggc atacgttccg gcacgcgcgg
     2941 actgatggcc ctggttgata tcccacgcgt cagcctgagt tttgtcgagg ttgccataca
     3001 agcggaagct gaattcgtcg cccagcggac cggtcaggct aaagttagtg cgtttggtgg
     3061 caccttcctc tttatgttct ggcgcattga aatatgcgtc ccaggagccg tgccactcgc
     3121 cgctgccttt tttggtaatg atgttaacca cgccgcccgc cgcgccgttg ccataacgcg
     3181 cagctgccgg accacgcaga acttcaatac gttcaatcat ttcaggtggc acccaggagg
     3241 tatcaccacg ggtatcgcgc tcgccacgcc agccctgacg caccgagtta cggctgctta
     3301 ccggcttgcc gtcaatcaaa atcagcgtgt tttccggacc cataccgcga atatcaatct
     3361 gtcggttatt cccacgctga ccactggtgg agttaccggt caggttaacg cctggcatgg
     3421 tacggatgat ctccgacaca tcgcgggcaa ccgggttttt gcggatttca tctgcggtga
     3481 tggtcgaaac gccaggagcc tgcaagttct gctcggcggc ggtaacgaca atggtatcgt
     3541 catgtgaaac aggtgtatcg gtcggctctt gtgcctgcgc taccccataa atccccagat
     3601 tgaccaacaa ggccagggaa tgaatcttct tgttcattgt tttattcctg catttttgcc
     3661 acgaattgca actgtcgggc atggtcgtca tcaacacgac gcatcccgtt accgcgaaaa
     3721 cctttgatcc tgaaagacac gcagtgcagt tggttaatta atgtccgcgc ttcccacggc
     3781 gcgccattac gctattgcaa atgcaaatag ttattaataa cattatcaat atatttctgc
     3841 aatcaatgaa aaattgcaca gtaaacatgg ggttatggtg tgacggcgtt aaaagtagga
     3901 agtgagagct ggtggcagtc gaaacatggc ccggaatggc agcgtctgaa tgacgaaatg
     3961 tttgaggtca ctttctggtg gcgtgatccc caaggttctg aagaatactc gacgataaag
     4021 cgcgtatggg tctacatcac tggtgtgacc gatcatcatc agaacagcca gccccagtcg
     4081 atgcagcgaa ttgcaggtac tgatgtctgg cagtggacga cacaactcaa tgccaactgg
     4141 cgcggcagct actgctttat tcccaccgaa cgcgatgaca ttttttctgc accatccccc
     4201 gatcgcctcg aattacgtga aggctggcga aaactattac cccaggcaat agccgatccg
     4261 ctgaatccac aaagctggaa aggcgggcta gggcacgctg tttctgcact cgaaatgccg
     4321 caagcgcctc tgcaaccggg atgggattgt ccgcaagcgc cagaaatacc agccaaagaa
     4381 attatctgga aaagtgaacg gttgaaaaat tcacgtcgtg tatggatttt taccaccggc
     4441 gatgtaacag cagaagaacg cccgctggca gttttgctcg atggcgaatt ttgggcgcaa
     4501 agtatgcccg tctggcctgt gctgacttcg ctgacccatc gtcagcaact tcctcccgcc
     4561 gtgtatgtgt tgatcgacgc tatcgacacc acgcaccgcg cccacgaact gccgtgtaat
     4621 gcggatttct ggctggcagt acagcaagag ttattacccc tggtgaaagt tattgcccct
     4681 tttagcgatc gcgccgatcg caccgtggtc gccgggcaga gttttggtgg gctgtccgcg
     4741 ctgtatgccg gactgcactg gcctgaacgc tttggctgtg tattaagcca gtcaggatcg
     4801 tactggtggc cgcatcgggg cgggcagcaa gagggcgtgt tacttgaaaa gctaaaagct
     4861 ggtgaagtta gcgccgaagg tctgcgcatt gtgctggaag cgggtattcg cgagccgatg
     4921 atcatgcggg ccaatcaggc gctgtatgcg caattacacc ctataaaaga atccattttc
     4981 tggcgtcagg ttgacggcgg acatgatgcg ctttgttggc gcggtggctt gatgcagggg
     5041 ttaatcgacc tctggcaacc acttttccat gacaggagtt gaatatggca ttcagtaatc
     5101 ccttcgatga tccacaggga gcgttttaca ttttgcgcaa tgcgcagggg caattcagtc
     5161 tgtggccgca acaatgcgcc ttaccggcag gctgggacat tgtgtgtcag ccgcagtcac
     5221 aagagtcctg ccagcagtgg cttgaagccc actggcgtac tctgacaccg gcgaatttca
     5281 cccagttgca ggaggcacaa tgagccagca tttacctttg gtcgccgcac agcccggcat
     5341 ctggatggca gaaaaactgt cggatttacc ctccgcctgg agcgtggcgc attacgttga
     5401 gttaaccgga gaggttgatg cgccattact ggcccgcgcg gtggttgccg gattagcgca
     5461 agcagatact ttgcggatgc gttttacgga agataacggc gaagtctggc aatgggtcga
     5521 tgatgcgctg atattcgaac tgccagaaat tatcgacctg cgaaccaata ttgatccgca
     5581 cggtactgcg caggcattaa tgctggcgga tttgcaacaa gatttgcgcg tcgatagcgg
     5641 taaaccactg gtctttcatc aactgataca ggtggcagat aaccgctggt actggtatca
     5701 gcgttatcac catttactgg tcgatggctt cagttttccg gccattaccc gccagatcgc
     5761 caacatttac tgcgcattgc tgcgtggcga accaacgcct gcttcgccgt ttacgccttt
     5821 cgctgatgta gtggaagagt accagcaata ccgcgaaagc gaagcctggc agcgtgatgc
     5881 ggcattctgg gtggaacagc gtcgccaact gccaccgccc gcgtcacttt ctccggcacc
     5941 tttagcgggg cgcagcgcct cggcagatat tttgcgtctg aaaatggaat ttaccgacgg
     6001 ggaattccgc cagctggcta cgcaactttc aggtgtgcag cgtaccgatt tagcccttgc
     6061 gctgacggct ttgtggctgg ggcgattgtg caatcgcatg gactacgccg ccggatttat
     6121 ctttatgcgt cgactgggct cggcggcgct gacggctacc ggacccgtgc tcaacgtttt
     6181 gccgctgggt attcatattg cggcgcaaga aacgctgccg gaactggcaa cccgactggc
     6241 aacccgactg gcagcacaac tgaaaaaaat gcgtcgtcat caacgttacg atgccgaaca
     6301 aattgtccgt gacagcgggc gagcggcagg tgatgaaccg ctgtttggtc cggtactcaa
     6361 tattaaggta tttgattacc aactggatat tcctggtgtt caggcgcaaa cccataccct
     6421 ggcaaccggt ccggttaatg accttgaact ggccctgttc ccggatgaac acggtgattt
     6481 gagtattgag atcctcgcca ataaacagcg ttacgatgag ccaacgttaa tccagcatgc
     6541 tgaacgcctg aaaatgctga ttgcccagtt cgccgcggat ccggcgctgt tgtgcggcga
     6601 tgtcgatatt atgctgccag gtgagtatgc gcagctggcg cagatcaacg ccactcaggt
     6661 tgagattcca gaaaccacgc tgagcgcgct ggtggcagaa caagcggcaa aaacaccgga
     6721 tgctccggcg ctggcagatg cgcgttacct gttcagctat cgggaaatgc acgagcaggt
     6781 ggtggcgctg gcgaatctgc tgcgtgagcg cggcgttaaa ccaggggaca gcgtggcggt
     6841 ggcactaccg cgctcggtct ttttgaccct ggcactccat gcaatagttg aagctggcgc
     6901 ggcctggcta ccgctggata ccggctatcc ggacgatcgc ctgaaaatga tgctggaaga
     6961 tgcgcgtccg tcgctgttaa ttaccaccga cgatcaactg ccgcgcttta gcgatgttcc
     7021 caatttaacc aacctttgct ataacgcccc gcttaccccc cagggcagtg cgccgctgca
     7081 actttcacaa ccgcaccaca cggcttatat catctttacc tctggctcca ccggcaggcc
     7141 gaaaggggta atggtcgggc agacggctat cgtcaaccgc ctgttgtgga ttcaaaatca
     7201 ttatccgctc acaggcgaag atgtcgttgc ccaaaaaacg ccgtgcagtt ttgatgtctc
     7261 ggtgtgggag tttttctggc cgtttattgc cggggctaaa ctggtgatgg ctgaaccgga
     7321 agcgcaccgc gacccgctcg ctatgcagca attctttgcc gaatatggcg taacgaccac
     7381 gcactttgtg ccgtcgatgc tggcagcatt tgttgcctcg ctgactccgc aaaccgctcg
     7441 ccagagttgc gtgacgttga aacaggtttt ctgtagtggt gaggccttac cggctgattt
     7501 atgccgcgaa tggcaacagt taaccggcgc gccgttgcat aacctgtatg gcccgacgga
     7561 agcggcggta gatgtcagct ggtatccggc ttttggcgag gaactggcac aggtgcgcgg
     7621 cagcagtgtg ccgattggtt atccggtatg gaatacgggt ctgcgtattc ttgatgcgat
     7681 gatgcatccg gtgccgccgg gtgtggcggg tgatctctat ctcaccggta ttcaactggc
     7741 gcaggggtat cttggacgac ccgatctgac cgccagccgc tttattgccg atccttttgc
     7801 tcctggtgaa cggatgtatc gtaccggaga cgttgcccgc tggctggata acggcgcggt
     7861 ggagtacctc gggcgcagtg acgatcagct aaaaattcgc gggcagcgta tcgaactggg
     7921 cgaaatcgat cgcgtgatgc aggcgctgcc ggatgtcaaa caagccgtta cccacgcctg
     7981 tgtgattaac caggcggcag ccactggtgg tgatgcgcgt cagttggtgg gctatctggt
     8041 atcgcaatcg ggcctgccgt tggataccag cgcattgcag gcgcagcttc gcgaaacatt
     8101 gccgccgcat atggtgccag tcgttctgct gcaactgccg cagttgccac ttagcgccaa
     8161 cggcaagctg gatcgcaaag ccttaccgtt gcctgaactg aaggcacaag cgccagggcg
     8221 tgcgccgaaa gcgggcagtg aaacgattat cgccgcggca ttcgcgtcgt tgctgggttg
     8281 tgacgtgcag gatgccgatg ctgatttctt tgcgctgggc ggtcattcgc tactggcaat
     8341 gaaactggca gcgcagttaa gtcgacagtt tgctcgtcag gtgacgccgg ggcaggtgat
     8401 ggtcgcgtca accgtcgcca aactggcaac gattattgat ggtgaagagg acagctcccg
     8461 gcgcatggga ttcgaaacca ttctgccgtt gcgtgaaggt aatggcccga cgctgttttg
     8521 tttccatccg gcgtccggtt ttgcctggca gtttagcgtg ctctcgcgtt atctcgatcc
     8581 acaatggtcg attattggca ttcagtcgcc gcgccctcat ggacccatgc agacagcgac
     8641 aaacctggat gaagtctgcg aagcgcatct ggcaacgcta cttgagcaac aaccgcacgg
     8701 cattgcggcg cggctgcgtg cccgtggcga acaggtggca tttcttggtt tgctggatac
     8761 ctggccgcca gagacgcaaa actggcagga aaaagaggct aatggtctgg acccggaagt
     8821 gctggcggag attaaccgcg aacgcgaggc cttcctggcg gcacagcagg gaagtacttc
     8881 aacggagttg tttaccacca ttgaaggcaa ctacgctgat gctgtgcgcc tgctgacgac
     8941 tgctcatagc gtaccgtttg atggtaaagc gacgctgttt gttgctgaac gcacgcttca
     9001 ggaaggtatg agccccgaac gcgcctggtc gccgtggatt gcggagctgg atatctatcg
     9061 tcaggattgt gcgcatgtgg atattatctc tccaggggcg tttgtaaaaa ttgggccgat
     9121 tattcgcgca acgctaaaca ggtaaattaa tattatct
//
LOCUS       DAJTRN010000136         9141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRN010000136 DAJTRN010000000
VERSION     DAJTRN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            384..1502
                     /locus_tag="OO328_003555"
     CDS             384..1502
                     /locus_tag="OO328_003555"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5931978.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1568..1816
                     /locus_tag="OO328_003556"
     CDS             1568..1816
                     /locus_tag="OO328_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5931979.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1881..2249
                     /locus_tag="OO328_003557"
     CDS             1881..2249
                     /locus_tag="OO328_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5931980.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2343..2996
                     /gene="nfsB"
                     /locus_tag="OO328_003558"
     CDS             2343..2996
                     /gene="nfsB"
                     /locus_tag="OO328_003558"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5931981.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3104..4351
                     /gene="ybdG"
                     /locus_tag="OO328_003559"
     CDS             3104..4351
                     /gene="ybdG"
                     /locus_tag="OO328_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5931982.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4419..5795)
                     /gene="pheP"
                     /locus_tag="OO328_003560"
     CDS             complement(4419..5795)
                     /gene="pheP"
                     /locus_tag="OO328_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5931983.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5896..9039)
                     /gene="cusA"
                     /locus_tag="OO328_003561"
     CDS             complement(5896..9039)
                     /gene="cusA"
                     /locus_tag="OO328_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5931984.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
BASE COUNT         2254 a         2530 c         2202 g         2155 t
ORIGIN      
        1 ggttgataga aatatctcct ggggcttcat ctttccagac ctcagagtaa acctgaaatc
       61 ggaaagcttc aggcacccgc agttatctta cccttcctca acaaaatagc tgtttattta
      121 tacagcaaat cctgtttcgg acttctcact ccccatttga tcaactgaag ctgaacccgt
      181 ttttttctgc atgcctcaat cgtcaaaatt cttcgccacc agccagctct gccataactt
      241 gccacattga aaaagtagcc aaagtgcttc aggcaccatc agttcaccat caccaaaaaa
      301 aagaaacatt ccagaacttc cgcatctcgc ccagccgctc cgacttctat actgaataga
      361 aaacgtcaac ttaagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta
      421 ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac ttaagccagg
      481 actcttcaat gctgattgac gcggttaaaa ataagatcac ggccggagag gtaaagcacg
      541 atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg
      601 ccgggcagtt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac catcatctgg
      661 aaatttgcgg cggtggcacg cccccgtttc agaaatggca gcgtcaggag gtatgcgaca
      721 acgaacgcta tcaacgaacg ctggaaaact ttggctatct catccagcag gcgaccgttt
      781 ttggtcagca tgtccatgtt ggctgtgcca gtggcgatga cgccatttat ttgctgcacg
      841 gcttgtcacg gtttgtgccg cactttattg ccctttccgc cgcgtcgcca tatatgcagg
      901 gaacggatac gcgttttgcc ccctcacgac cgaatatttt ttccgccttt cctgataatg
      961 gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc tgtctgagtt
     1021 acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc agtcctcatt
     1081 ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata
     1141 tggcgggatt aattcaggcc accgcccact ggttactgac ggaacgcccg ttcaaacatc
     1201 aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgtcgc tatggtctgg
     1261 aaggcgtcat taccgatccg cacactggcg atcgtcgacc gctaacggaa gataccttgc
     1321 gattgctgga aaaaatcgcc ccttcagcac ataaaattgg cgcatcgagc gcaattgaag
     1381 cactgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc gatttcgtcg
     1441 ccgatggcgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg gccggtgact
     1501 aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
     1561 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
     1621 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
     1681 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
     1741 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
     1801 ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc
     1861 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
     1921 ctcttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
     1981 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
     2041 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
     2101 ggtatcacat gaataaaaag cactggattt cggtgtattc cggcgaggaa atctccgaag
     2161 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
     2221 gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct atagcgcatt tttctcgctt
     2281 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attacttaca catggagtct
     2341 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
     2401 aaaaacttac cccggaacag gccgagcaga tcaaaacgct actgcaatac agcccatcca
     2461 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
     2521 ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg taaaatactt gatgcctcgc
     2581 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
     2641 accaggaaga tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
     2701 gtcgcaagtt cttcgcctat atgcaccgta aagatctgca tgatgatgca gagtggatgg
     2761 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
     2821 acgcggtgcc catcgaaggt tttgacgccg caatcctcga tgcagaattt ggtctgaaag
     2881 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
     2941 acgctacgct gccgaaatct cgtctgccgc aaaacaccac cttaaccgaa gtgtaattct
     3001 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
     3061 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
     3121 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
     3181 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
     3241 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
     3301 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
     3361 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
     3421 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
     3481 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
     3541 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
     3601 gattctaatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
     3661 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
     3721 ctggctggaa atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
     3781 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
     3841 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
     3901 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
     3961 taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa
     4021 tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac
     4081 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
     4141 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
     4201 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
     4261 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
     4321 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
     4381 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
     4441 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
     4501 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg
     4561 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgccgcgcga
     4621 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcaacgttgc taccaccagc
     4681 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
     4741 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
     4801 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
     4861 accccgctgt tatacaccga cagcgatgcc actagaatga cgaagttcag cgcagaagct
     4921 accacattgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
     4981 tccacccacg gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcaggatg
     5041 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
     5101 gctgtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
     5161 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
     5221 gctttctcgc cgccgtaacc agaaaacagc agccacaggc caaagccgat cataccgatg
     5281 atcgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc
     5341 accagattaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
     5401 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
     5461 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
     5521 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
     5581 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
     5641 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
     5701 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
     5761 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
     5821 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
     5881 acgaggtatc ctgctttaat tccgtacccg atgtcggtgc agccacatca gcttatacgc
     5941 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     6001 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     6061 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     6121 caacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggctcggc
     6181 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     6241 aagggcaata aagccggtac ccgtcgccac agaaagatga aagcccatcc accacagtaa
     6301 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     6361 ccgacggaat gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag
     6421 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     6481 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     6541 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     6601 cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg
     6661 cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc gccagctttg
     6721 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     6781 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     6841 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcagcaag
     6901 cgcagaagct acgcctggca ccgttcgcgc cacttcttca atttgctcag ccatcgcgtc
     6961 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     7021 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg
     7081 cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc gccactgatc
     7141 ctgtggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     7201 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     7261 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     7321 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     7381 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     7441 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcggattact
     7501 gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga tcactacgat
     7561 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     7621 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     7681 actgataaac agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     7741 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagct gtttatgcgc
     7801 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     7861 cagcgacata atattggcat tcagcccctg gaagggcatg acaataaaag caatacacaa
     7921 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacggcgc
     7981 gcagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     8041 ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc tccacgcctt ccggcagact
     8101 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     8161 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     8221 ccggcgcatc ttcgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     8281 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct
     8341 ggcgcgcacc atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt
     8401 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     8461 aggatcgata accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac
     8521 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     8581 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     8641 ggcatctggt cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     8701 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     8761 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
     8821 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg
     8881 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt
     8941 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     9001 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     9061 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     9121 atcaggaaca ggccgccgga a
//
LOCUS       DAJTRN010000137         9088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRN010000137 DAJTRN010000000
VERSION     DAJTRN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            112..768
                     /gene="pdxH"
                     /locus_tag="OO328_003562"
     CDS             112..768
                     /gene="pdxH"
                     /locus_tag="OO328_003562"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5931985.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            897..2171
                     /gene="tyrS"
                     /locus_tag="OO328_003563"
     CDS             897..2171
                     /gene="tyrS"
                     /locus_tag="OO328_003563"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5931986.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2230..3093
                     /gene="pdxY"
                     /locus_tag="OO328_003564"
     CDS             2230..3093
                     /gene="pdxY"
                     /locus_tag="OO328_003564"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5931987.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3137..3742)
                     /gene="gstA"
                     /locus_tag="OO328_003565"
     CDS             complement(3137..3742)
                     /gene="gstA"
                     /locus_tag="OO328_003565"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5931988.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3848..5350)
                     /gene="dtpA"
                     /locus_tag="OO328_003566"
     CDS             complement(3848..5350)
                     /gene="dtpA"
                     /locus_tag="OO328_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5931989.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(5961..6596)
                     /gene="nth"
                     /locus_tag="OO328_003567"
     CDS             complement(5961..6596)
                     /gene="nth"
                     /locus_tag="OO328_003567"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5931990.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6596..7291)
                     /gene="rsxE"
                     /locus_tag="OO328_003568"
     CDS             complement(6596..7291)
                     /gene="rsxE"
                     /locus_tag="OO328_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5931991.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7295..7915)
                     /gene="rsxG"
                     /locus_tag="OO328_003569"
     CDS             complement(7295..7915)
                     /gene="rsxG"
                     /locus_tag="OO328_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5931992.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7919..8977)
                     /gene="rsxD"
                     /locus_tag="OO328_003570"
     CDS             complement(7919..8977)
                     /gene="rsxD"
                     /locus_tag="OO328_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5931993.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2328 a         2280 c         2306 g         2174 t
ORIGIN      
        1 aacgcgaccg catcgtcttg aataactgtc agttacaaaa tccacagcgt tgagattttt
       61 ccaggggcgg cgcacaatag cgtcacccac tgacaatccg taaagaaaac catgtctgat
      121 aacgacgaat tgcagcaaat cgcgcatctg cgccgtgaat acaccaaagg cgggttacgc
      181 cgccgcgatc ttcccgccga tccattaacc ctttttgaac gttggctctc tcaggcttgt
      241 gaagccaaac tggcggaccc taccgcgatg gtggtcgcta ccgtggatga acatggtcag
      301 ccttatcagc gcatcgtttt actcaaacat tacgacgaaa aaggcatggt gttttacacc
      361 aacctcggta gccgtaaagc acatcaaatt gaaaataatc cgcgcgttag cctgctgttc
      421 ccgtggcata cccttgaacg ccaggtgatg gtgatcggta aagcagaacg actttcgact
      481 ctcgaagtga tgaaatattt tcatagccgc ccgcgtgata gccagattgg tgcatgggtt
      541 tcgaagcagt ccagtcgcat ttctgcccgc ggtatccttg aaagtaaatt cctggagctg
      601 aagcagaagt ttcaacaggg cgaagtgcca ttgccgagct tttggggcgg ttttcgcgtc
      661 agccttgaac agattgagtt ctggcagggc ggtgagcatc gcctgcatga ccgctttttg
      721 taccagcgtg aaaatgatgc gtggaagatt gatcgtcttg caccctgaaa agatgcaaaa
      781 atcttgcttt aatcgctggt actcctgatt ctggcacttt attctatgtc tctttcgcat
      841 ctggcgaaaa gtcgtgtacc ggcaaaggtg cagtcgttat atacatggag attttgatgg
      901 caagcagtaa cttgattaaa caattgcaag agcgggggct ggtagcccag gtgacggacg
      961 aggaagcgtt agcagagcga ctggcgcaag gcccgatcgc gctctattgc ggcttcgatc
     1021 ctaccgctga cagcttgcat ttggggcatc ttgttccatt gttatgcctg aaacgcttcc
     1081 agcaggcggg ccacaagccg gttgcgctgg taggcggcgc gacgggtctg attggcgacc
     1141 cgagcttcaa agctgccgag cgtaagctga acaccgaaga aactgttcag gagtgggtgg
     1201 acaaaatccg taagcaggtt gccccgttcc tcgatttcga ctgtggagaa aactctgcta
     1261 tcgcggcgaa caactatgac tggttcggca atatgaatgt gctgaccttc ctgcgcgata
     1321 ttggcaaaca cttctccgtt aaccagatga tcaacaaaga agcggttaag cagcgtctca
     1381 accgtgaaga tcaggggatt tcgttcactg agttttccta caacctgttg cagggttatg
     1441 acttcgcctg tctgaacaaa cagtacgatg tggtgctgca aattggtggt tctgaccagt
     1501 ggggtaacat cacttctggt atcgacctga cccgtcgtct gcatcagaat caggtgtttg
     1561 gcctgaccgt tccgctgatc accaaagcag acggcaccaa atttggtaaa actgaaggcg
     1621 gcgcagtctg gttggatccg aagaaaacca gcccgtacaa attctaccag ttctggatca
     1681 acactgcgga tgccgacgtt taccgcttcc tgaagttctt cacctttatg agcattgaag
     1741 agatcaacgc cctagaagaa gaagataaaa acagcggtaa agcaccgcgc gcccagtatg
     1801 tactggcgga gcaggtgact cgtctggttc acggtgaaga aggtttacag gcggcaaaac
     1861 gtattaccga atgcctgttc agcggttctt tgagtgcgct gagtgaagcg gacttcgaac
     1921 agctggcgca ggacggcgta ccgatggttg agatggaaaa gggcgcagac ctgatgcagg
     1981 cgctggtcga ttctgaactg caaccttccc gtggtcaggc gcgtaaaact atcgcctcca
     2041 atgccatcac cattaacggt gaaaaacagt ccgatcctga atacttcttt aaagaagaag
     2101 atcgtctgtt tggtcgtttt accttactgc gtcgcggcaa aaagaattac tgtctgattt
     2161 gctggaaata atgcattaag tggaaagggg gagtgagaaa tcactccccc tggtttttat
     2221 acagggaaca tgatgaaaaa tattctcgct atccagtctc acgttgttta tggtcatgcg
     2281 ggtaacagtg cggcagagtt tccgatgcgc cgcctgggcg cgaacgtctg gccgctgaac
     2341 accgttcaat tttctaatca cacccaatac ggcaaatgga ctggctgcgt gatgccgccc
     2401 agccatttaa ccgaaattgt gcaaggcatt gccgccattg ataaattaca cacctgtgat
     2461 gccgtattaa gtggctatct gggatcggcg gagcagggtg aacatatcct cggtatcgtc
     2521 cgtcaggtga aagcagcgaa tccgcaggcg aaatattttt gcgatccggt aatgggtcat
     2581 ccggaaaaag gctgtatcgt tgcaccgggt gtcgcagagt ttcatgtgcg gcacggtttg
     2641 cctgccagcg atatcattgc gccgaatctg gttgagctgg aaatactctg tgagcatgcg
     2701 gtaaataacg tcgaagaagc ggttctggca gcgcgcgaac tcattgagca agggccacaa
     2761 attgtgttgg ttaaacacct ggcgcgagct ggctacagcc gtgaccgttt tgaaatgctg
     2821 ctggtcaccg ccgatgaagt ctggcatatc agccgtccac tggtggattt tggtatgcgc
     2881 cagccggtag gtgttggtga tgtgacgagc ggtttactgc tggtgaaact gcttcagggg
     2941 gcaacgctgc aggaggccct ggaacatgtg accgctgcag tctacgaaat catggtgacc
     3001 accaaagcaa tgcaggaata tgagctgcaa gtggtggctg ctcaggatcg tattgccaaa
     3061 ccagaacatt acttcagcgc aacaaagctc tgaaatatct catttcggcc cacaacgacc
     3121 tgtgggccga tggctattac tttaagcctt ccgctgacag cgcgtcttgt acttccggac
     3181 gttcagccat acgttgcata aatgccgcaa tgtgctctaa cccttccaga ttcagtttca
     3241 ccgcgtatgc ccagcgcaga accgtaaaca gataggcatc agcaattgta aatctttgcc
     3301 cgcagatcca gtgctcatcc ttcagtgcct cgttcacata ttgcagcttc ttatccagct
     3361 gcgcgcgaac tgtcggtttg tactcttccg gtgtatctgg gcgaaacaga ggtgtgaaac
     3421 ctttatgcag ctcggtggcg atgtaattca gccattcgat ggttttatag cgggaaatac
     3481 tgtttaccgg tgccagcaac tggcggtcgg ggacgctgtc ggcaagatac tgcataatcg
     3541 caacgccttc cgtcagcaaa gtaccatcgt ccagcagcaa tgcaggcacc tgccccttag
     3601 ggttaacgga aaagtaatcg tcaccgtttt cgagacgttt tttcattaaa tccacactga
     3661 cgagcgtaaa atcctttccg ctctcacgca gggtgatatg ggaagcgaga gagcaggcgc
     3721 ccggtttgta gaacaatttc atcggtaact ccttttgtct gtggtttaaa gtatggtagt
     3781 gcgttgctga acaaaaaaaa agccgctaac gaaaagttag cggcttgtaa aagagtttcc
     3841 ctgaatttta cgctacagta gctttcgccg ctttgtctgc agcgtcatcc tgcgtcatgc
     3901 ggtgcagttt aggcgcggtc agcagcatca gtactgcaat aacggcagta gcgacaccaa
     3961 tctgcaagaa tacgcgacca tagacttcca gtgacatcag cgggtcggta acgttatccg
     4021 gcacagccat catacccgca acataaccac caatcaggtt tgcaccggca gtggtcagga
     4081 accagctacc cataatgaag cccatcagac gctgcggaac gagttgtgca accattgcca
     4141 gacccagacc agagatcatc agttccccga tgctctgcag gccatagctt gcgaccagcc
     4201 agctgacaga cacgatacct gcgtcagacg cgaatttcgc acccagcggc agaatcagga
     4261 acgcaccaga acacatcacc atgccgattg caaacttggt tggcatcggc agggtatcac
     4321 ccatcttgtt atagatagcg gccagaatcg gactaccgat gatgatccag aacgggttca
     4381 gtgcctgata ctgctcaggt tctacggcca gacccagaat ggagtgctca acgttacgaa
     4441 tcgcaaagaa gttcagtgac gttggcatct ggctgtacag cacgaagaag atgattgctt
     4501 cgagcatcag gatgaaggca acgatcattt tacgacgcgc agcacctttc atcgcgaaag
     4561 cttctttacc gaagataacc acaataccga aggcaacaac gcccagcgcc atacgcgcaa
     4621 cttcctgatt gtgcagtagc caggtggcaa cagcgatcag cgccacaaca ccaataatgg
     4681 tcagcaacag gttacggtag ttgactggtt caaagtctgg ttttgaaccg tactgtttaa
     4741 cccagcgttg gcagaaggcg aagttaacga tagtgatcag caggcctaca acgctcaacg
     4801 caaacgcaac actccagccg tatttcgcgg ccagccacgg ggtggctatc atagagaaga
     4861 aagagccgat gttgacggac atgtagtaca tggtgaatgc accgtccaga cgtgggtcgt
     4921 ttttctcata gcatgtagaa agcagagaag acgggttagc tttaaacagg ccgttaccga
     4981 ccgcaatagc cgccataccc atataaacaa taccggcgtc gtgaccagac caggcaacca
     5041 gagcataacc aatcgccagc acaatagcgc cgagcataat tacgcgttta gtacccagta
     5101 ccttgtcacc taaccagccg ccgatagcga ccagaccata aaccagggca ctaaaggaag
     5161 agaaaagggt gattgaatcc gcttcagaca tacccagttg tttaaccagg taaacagcca
     5221 taattccttg taggccgtaa taaccaaaac gttcccataa ctcaatcgag aagatgagat
     5281 agaacgcctt cggttgtttg aaagcgttca aactgacgct ttcagttggt ttttggtttg
     5341 cagtggacac ttttacctct ttttttacat cccatattaa cgggggtgct ttctcgcgtt
     5401 atgtctaaaa aacatacgcg caattgttat agtgggaggg gaaacggcag gtaatgttca
     5461 ctatcttgca ctttcagaca agtcctttgt aataatctgt tacatataac tgaggcgggg
     5521 aattcaacca ttccgggaaa tgttatccag cgttaaattt ttcaaaaaac gtaacggcag
     5581 attttttcac tgcattgaga gggggatgat ggcttggcgg cgatatgttc tgctatttag
     5641 cgtttttcct agtgagatag tccagtttct gaaaaatagc cagtgtaatg ttttgcaggt
     5701 caatatttgt tgcgtgtgta aatttaaatc attttaacct gattcttgca atgtttttac
     5761 attgccacag catcgtgatc ttgatcacga atgaatagaa aatttcgcta ttaaaaaaac
     5821 aattaacctg catttttggt tatccgcaaa cggattacct gatgataacg ctaagcaatg
     5881 gcatcattga cgttaaaaag atgttttaaa agaggataaa gaaaggttat caatggggta
     5941 atcggtgtta ccccttttct tcagatgtca actttctctt tgtattcaca aagatcttca
     6001 ataatacaag agccacagcg gggcttgcgg gcaatgcagg tataacgccc gtgcaggatc
     6061 aaccaatggt ggcagtcgac tttaaactct gccggaacca ctttcagtag cttttcttct
     6121 acctgttcga cgtttttccc cggcgcaaat tgagtacgat tacaaacgcg gaaaatgtgc
     6181 gtgtcgacag caatagtcgg ccagccgaat gcagtgttta atacgacgtt ggctgtttta
     6241 cgacctacgc cgggcagggc ttcaagcgca gcacgatctt ccggaacctc gccattatgt
     6301 tgttccagta atatacggca ggttttgatg atattttctg ctttgctgtt ataaaggcca
     6361 atcgttttga tataggtttt caccccttca acgcctagtt caagcatcgc tgcaggcgta
     6421 ttcgccaccg ggtagagttt cgccgtcgcc ttattaacac tgatatcggt cgcctgagcg
     6481 gaaagcagta cggcaatcag caattcaaaa ggcgaactga aattaagctc ggtggtggga
     6541 tgaggattgt tctcacgcag gcgagtgagg atctccagac gttttgcttt gttcatcaga
     6601 cattccctgt ttcaccgttt ggcaatgcac gttcggcagc tgcttcagca cggcgctttt
     6661 tcattctttc atcaatcagg tattttcctg ccagcatcag tcccaagcca ataaatgcac
     6721 ctggtggcag catcgccagc aggaaagggg agtcggtgtg gaaaatctcc acgcgtaata
     6781 cttttgccca gctacctaac agcgcatctg caccgtcaaa caacgtgcca ttgccgataa
     6841 tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc gcccgtacca attgaaaagc
     6901 cgtccagtgc cgaaagcgcc ggaccttttt tggcggcgaa ggcttcagcg cggcccacaa
     6961 cgatacagtt agtgacaatc agcggaataa aaatccctaa tgattgatac aggccaaagg
     7021 cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga ggcgatgatc atcacgtaaa
     7081 tgggaatgcg gatctcggct ggcgtccagt gacgcagcgt cgaaatggtc aggttggtca
     7141 gcgtcagtac cagcgtagtc gcaagtccta aacccagagc gttagtggca gtggatgtga
     7201 ccgccaatag cggacaaagg ccgagcagtt ggaccagtgc agagttgttt ttccacaacc
     7261 cctgaacaat aacgtcttta atttcgctca cgctttattc tccacaggca ggaagttgag
     7321 aaagttgtgc cggtaacgtc tgagcgtaca atccggcgcg ttttaccgca ttaaccaccg
     7381 cgcggggagt aatcgtcgcg ccggtgaact ggtcgaaatc accaccatct ttcttcaccg
     7441 cccagtgcgc atcatccgca ccactgattt tttttcccgc aaaatgggtg agccagtcag
     7501 aaaggcgcaa ttcgatttta tcgccaagcc ctggcgtttc gtggtgctct gtcacgcgcg
     7561 tgccaagtac cgtgccatta aaatcggctc ccaccagcag ctgaatcgca ccggaatagc
     7621 catctggcgc ggttgcttcc agaacggcgg ctaccggttt gtcatcctgt ttggcgatgt
     7681 aaacccgatg ctcaccttta cctaactctg gcgcagttac cagatagcaa ctctgtgcga
     7741 gcgcattgtt atagcgttcg gctggcagca cctgatcaaa taacgtcttt tgttgcagac
     7801 tggcctgttc agcaattgtc gttttggtca tctggttgat ggccgcagtt aaccctgtag
     7861 aacccgctgc aaatagtgcc agtgtaatgc cgtgttttcg gatagttttc agcatggttt
     7921 accctttgcg atggccgtag acgcgcggac gcgtgtagta atcgatcaga ggaaccgtga
     7981 tgttcgccag caggacggca aaagccacgc cgtcaggata gccgccgaaa ctgcggatca
     8041 accagactaa taagcccgcc agcgcgccga acatcagccg accacgattg gtcgtagaag
     8101 cagtaactgg gtcagtcaaa ataaagaatg cgccgagcat agtcgcacca gacagcagat
     8161 gaatttgcgg tgctgccagt gtttctggtg agaacaacca gcccaacgtt gcgcataacg
     8221 ccagcgttac taagaagctg aggggaatat gccagcgaat cgctttctgc catagcaacc
     8281 acacgccacc agccagccag gcgagattta cccattgcca accagcgccc gccagaatac
     8341 cgctgtagat cggatattgc ataatctgtt caaccgaatg acctgcgcgg acagaggttt
     8401 tgaatgtatc cagcggtgtc gcctgactaa tgccatcaat acctaagcgt agcgtattca
     8461 tatcaccacc actggcggta tgaccgctga aaataacctg tatggcgtcg ataaaaccag
     8521 ggatgttgac cgcaatttca tgtggcggta accagctggt catctgcacc gggaaggaga
     8581 tcagtaagac cacataacca atcattgccg gattaaacgg gttttgcccc agaccgccat
     8641 acaactgttt agcgatgatc accgcaaaca ccgtacccag tacgaccatc caccatggcg
     8701 ctaggggggg aatacttacc gccagcaata agcctgtcag caatgctgag ttatctttca
     8761 acgttgcggc taccgactgc ttgcgtagtt tgagtacgag agcttcggct aacagagcac
     8821 tgaccgacgc caacaggatc tgaacgagag taccccaacc aaaaaaccgc agttgcgctg
     8881 cgattcctgg cacggctgcg agcaacacca acagcataat gcgcgatgtc tggcgctggt
     8941 tatgggtata aggggagcta gctattctga ataccattta gtcctcgttt acaacctttt
     9001 gctgggcggc ttttttggcc tgaacgcggg caatagccgc ggcaactgcg gctttgcgcg
     9061 gatcgacctg ttcttctggt tccgcatt
//
LOCUS       DAJTRN010000138         9048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRN010000138 DAJTRN010000000
VERSION     DAJTRN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..799)
                     /gene="bglB"
                     /locus_tag="OO328_003571"
     CDS             complement(<1..799)
                     /gene="bglB"
                     /locus_tag="OO328_003571"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5931994.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGY"
     gene            complement(818..2695)
                     /gene="bglF"
                     /locus_tag="OO328_003572"
     CDS             complement(818..2695)
                     /gene="bglF"
                     /locus_tag="OO328_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5931995.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2829..3650)
                     /gene="bglG"
                     /locus_tag="OO328_003573"
     CDS             complement(2829..3650)
                     /gene="bglG"
                     /locus_tag="OO328_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5931996.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(3937..4662)
                     /gene="phoU"
                     /locus_tag="OO328_003574"
     CDS             complement(3937..4662)
                     /gene="phoU"
                     /locus_tag="OO328_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5931997.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4677..5450)
                     /gene="pstB"
                     /locus_tag="OO328_003575"
     CDS             complement(4677..5450)
                     /gene="pstB"
                     /locus_tag="OO328_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5931998.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5542..6432)
                     /gene="pstA"
                     /locus_tag="OO328_003576"
     CDS             complement(5542..6432)
                     /gene="pstA"
                     /locus_tag="OO328_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5931999.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6432..7391)
                     /gene="pstC"
                     /locus_tag="OO328_003577"
     CDS             complement(6432..7391)
                     /gene="pstC"
                     /locus_tag="OO328_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5932000.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7478..8518)
                     /gene="pstS"
                     /locus_tag="OO328_003578"
     CDS             complement(7478..8518)
                     /gene="pstS"
                     /locus_tag="OO328_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5932001.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8766..>9048)
                     /locus_tag="OO328_003579"
     CDS             complement(8766..>9048)
                     /locus_tag="OO328_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5932002.1"
                     /translation="DDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGE
                     LPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2299 a         2405 c         2135 g         2209 t
ORIGIN      
        1 tatagccggg atattggcca cgcgcctgaa catcaccaaa gaacatccag cgccggttct
       61 cttccatggc ctgcaacata tcctgtggct ggcaggtgag ggggtaaacc agcccaccga
      121 gaagcatatt gccgattttc gcttcgggga tcaggctatg ataggcttta actgcccgcg
      181 cactggcaac cagttgatga tggatcgcct gataaacttc cgcctcgcca ctctcttctg
      241 ccagccccac gcccgtgaat ggcgcgtgta acgacatatt gatttcatta aacgtcagcc
      301 ataacgctac tttatgttgg tagcgggtaa agaccgtgcg ggcgtaatgc tcgaagtgat
      361 cgatgaccgc ccgattagcc caaccgccgt agtttttcac cagcccatat agcatttcgt
      421 aatgggataa cgttaccagc ggcttgatcc ccgcctgcgc catttcatca aacagccgat
      481 cgtaaaacgc taaccccgct tcattcggtt cggcttcgtc gccctgaggg aaaattcgcg
      541 cccaggcaat ggaaatacgc agacaggtga agcccatctc ggcaaataac gcgatatctt
      601 ccgggtaacg gtaataaaaa tcgatggcga catctttgat attctctttc cccaggatgc
      661 gcggttccat ttttcccatt acgccatgag gctgtaaatc tgaggtcgtg atccctttgc
      721 catcttccag ccaggcacct tccacctgat tggcagctgt tgcgccaccc caaagaaatg
      781 tttctggaaa tgctttcata attaactcct tttatcgtta gcgaatgatg gataacagcg
      841 gttcacctgc gcttatctgc gccgtgccgt ggggtaatac gtctgtaaaa tcatcgctat
      901 tactgattaa taccggcgtc gtcagatcaa atccggcctc gcgaatagca gggatatcaa
      961 aagaaatcag ccgatcgcct gtattgacct tgtcacccac gttgacgtga gcggaaaaga
     1021 atttgccgtc cagttttacg gtgtcgatac cgacatgaat caggatctcc acaccatcat
     1081 ctgactcaat gccaatggcg tgtaatgtgg cgaacaacga agcaattcga cccgcaaccg
     1141 gagaacgcac ttcaccaacc gagggcagaa tggcgatacc tttacccaac aggccactgg
     1201 caaacgtggt atcagcgacg tgaatgagcg gcacaatctc tcccgtcatc ggtgaacaga
     1261 taccgccctg ctcaggtggt gtaataacct ctggtgtttt ctcttgcggg gcaccctgcg
     1321 ctggctgacg tttactggtg atgaaatgaa gcatcaacgt accaacaaat gcgcaaccta
     1381 tggcaatgac accgccaata acgctggccc agacggtgaa atcaatcccc gttgacggga
     1441 tggtctgcat gaaggtgaaa atacttggca aaccaaagga gtaaactttc gtttgcgcgt
     1501 agccaatgat ggtggccccc aatgctccac tgatacaggc gataacaaag gggtacttac
     1561 gcggcaggtt gacgccatat accgctggtt cggtgatacc aaacagaccc gtcaacgccg
     1621 ctgatcccgc caccactttt ttctgcgcat cgcgttcgca gaggaagacg ccgagcgccg
     1681 ccccgacctg cgccataatg gcgggcatta acagcgggat catggtgtcg tagcccagca
     1741 cggtgaagtt attgatacac agcggcacca ggcccctgtg cagtccgaac atgacgaaga
     1801 tttgccagaa gccgcccatt accgcgcccg caaatgcagg aaccgcctga taaagccaga
     1861 gataaccggc ggcaatcatt tcgcttatcc aggttgatag ctgccccacc agcagaaagg
     1921 tgacgggtgt gataaccatc agacatagca atggtgtgaa gaaatttttg attgccgacg
     1981 gtaaccacgc attaagtcgg cgttccagaa tgctgcacaa ccaggcagaa aaaataatgg
     2041 gaataaccga tgacgagtaa ttcaacaatg tgaccggaat acccaggaaa tccagccccg
     2101 gcgcatccgc tttttgcccg ttctcgaagg cagtcagaat taatggatgc actaacgctc
     2161 caccaatcac catggcagta aatggattac cgccgaagcg tttgcccgcg gtgtatccca
     2221 ggattatcgg gaagaaccca aacaaggcat cactggcgct gaataaaatt aaataagtac
     2281 cactttgttc ggtcgtccac tgaaaagtga gcgccagagc cagcatacct ttcaggatcc
     2341 cggttgccgc catcagaccg atcagaggcg taaaaatacc tgaaataaca taaacaaagc
     2401 ggtttagcag attacctttc tcatcatttt ccggtgcctg ttgcgctttt tcgccaaggc
     2461 ctgccacgct gttaaccgcc aggaagacat cggccacatg gttacctatg accacctgaa
     2521 actgaccacc gctttccacc accataataa taccgggggt ctttttcagt acctcagctt
     2581 gcgctttgct ttcatccttt aacttaaaac gtaatcgcgt tgcgcaatgc atcagactca
     2641 caatgttatc tgcgcccccg actcctgcga ctatttttct ggctaactcc gtcataactt
     2701 gccctctacc gctttgcggc aaaactccaa aaaaaaacct gaaaaaaacg gcctgacgtg
     2761 aatcaagcaa tttttttcag gttttgcccg cttagtgcgg taacaatcct ttactcagta
     2821 ataatatttc agtgttcttt gcgcacgcgc tctatattta tggctaaaaa cataatctct
     2881 gcgggtgaaa ttttacgttg atactgcaaa ccaataaaaa tggcgatccg ttccgcacat
     2941 tgccatgctt gcgggtaatt ttgttttact gcttgttgta atgattcatc actatcgtta
     3001 attgaagcat gttcaagaat acgccaggat aaaaacttca gatgtgtaac cagtcgctga
     3061 taactcaagc tttcttcctg gtaattaagg ctgaactgaa attttattaa ttgcagcatt
     3121 tcgcgcatta actgcgtgac acctgcaaca tcctccatat ttccgctcat ttgggcactg
     3181 accagatgca tggcaataaa gcccacttca tctttcggta actgcacgcc caaccgttta
     3241 tcaataatgg ttaacgcttc ttcccctagc tggaactctt tcgggtaaag ccgctggata
     3301 tcccacagca acacgttttg ctgaaagcgt ttaatcgcaa actggcaatg gtcagttagc
     3361 gagatataaa tactgtcctg taattttccc aagcgctcct gcgctaaaga gataagacga
     3421 tcacaggttg ccatcacctc aagaggaata tgacttaaga gttcgcttaa tcgcccgttc
     3481 agttcatgac tgctcaaggc atactctttt tctattccac ttgagttaat tctttcgcca
     3541 gcgcgttttt gaaagccaat tccgcgcccc atgacgactt tttcccgctg ttgatcatca
     3601 ataaccacca caacattatt gttgagaatt ttggtgattt gcatgttcat agcaaggacc
     3661 ttttttataa acaaaaaaaa accgacttca ccagtattct ctggttatgt caggttttgc
     3721 ctgcgaatgc agtaacaatc cagtcattta ttaatggttt ttataacgaa catccaggtt
     3781 cggaaattaa tttaattgcg tgcagagaaa atagcaatgc gctattgata aaaatatgac
     3841 catgctcgca gttattaacc ttgtgtaatt ttaggaattt ataaagttat atataacaaa
     3901 tcccaataat taagttattg ggatttgtct ggtgaattat ttgtcgctat cttttcccgc
     3961 cagcagttta tccagctcat caccgcctac atgacggaaa tcctgaccct tcacgtagta
     4021 gaagataaac tcacaaatat tctggcagcg gtcgccgata cgttcgattg aacgcgcaca
     4081 gaacagcgcg gtaagtacgc tcggaatagt gcgcgaatct tccatcatgt aggtcatcag
     4141 ttgacgaaca ataccttcgt attcctgatc gactttttta tcttcacgat aaatacgtac
     4201 cgcttcgtca atgtccatcc gcgcgaacgc gtccagcacg tcgtgcagca tctggatggt
     4261 atgacggccc agcgactcca gacttaccag caacggctga tgctgctggg agaatttctc
     4321 cagcgcagta cggcagattt tgtccgccac gtcgccaata cgctccagct cggcaatggt
     4381 tttactgatc accataacca gtcgcaggtc gctcgccgtc ggctgacgtt tggcgataat
     4441 gcgcacgcag gcttcatcga tcgccacttc catcatgttg acgttcttgt cgccttcgat
     4501 cacgcgcttc gccagatcgc tgtcctggtt atgcatcgcg gtgattgcat cagaaagctg
     4561 ctgctccacc atgccgccca tggtcatcac ctgcgtgcgg atactttcca gttcggcgtt
     4621 gaactggccg gaaatatgtt tattaagatt gagactgtcc ataacgcact cctgaatcaa
     4681 ccgtaacgac cggtgatgta gtcttctgtt tgtttcttcg ctggcttggt gaacagatcg
     4741 tccgtgttgc tgaactcaat caattcgccc aggtacataa atgccgtgtg gtcggaacaa
     4801 cgcgcagcct gctgcatgtt gtgggtgacg atcaccacgg tgtaatcctg cttcagttcg
     4861 gtgatcagct cttcaatacg cccggtagag atggggtcga gcgccgaaca cggttcgtcg
     4921 agcagcagca cttccgggcg aatggcgata ccacgcgcaa tacacagacg ctgttgctga
     4981 ccaccagaga gagagtaacc gctctggtgc aatttatctt tggtttcgtt ccacaatgcg
     5041 gctttggtca atgcccactg cacgcgctcg tccatgtcgg cacgggagag cttctcaaac
     5101 agacgaacgc caaaggcgat gttgtcgtag atggacatcg ggaacggcgt cggtttctgg
     5161 aacaccatgc ccactttcgc acgcagcagg gcgatatcct gagagttggt caggatgttg
     5221 tcgccatcaa gcagaatttc accttccgca cgctgctccg ggtacagttc aaacattttg
     5281 ttgaaggtac gcagcagcgt cgatttaccg cagccggacg gcccgataaa cgccgtaacc
     5341 tggtttttgg cgatatccag gttgatgttt ttcagggcat ggaatttgcc gtagtagaag
     5401 ttcaaattac gaacctgaat tttactcggg gcagtttcaa ccatactcat tgcaatcttt
     5461 tcctcatcgt acctgatgct tttcgctaat caggcttacc gtagggccgg gtaaggcgtt
     5521 cacgccgcat ccggaaaagt gttaaccgtg tttattcttc gcaaaaacaa cgcgcgccag
     5581 aatgttcagc agcaggacgc acagggtaat gatcaatacc ccggcccagg ccagttgttg
     5641 ccattccgca aacgggctca tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg
     5701 ctgcatcatg tccgtgctcc agaactggtt ggagagcgcg gtaaacagca gcggcgcggt
     5761 ttcaccggca atacgggcaa tcgccagcag gataccggtc ataatcccgg acaccgacgc
     5821 tttcagcgta atcgcggaga tcatcttcca cttcggtgta cccagcgcat aagccgcttc
     5881 acgcaggctg tccggcacca gtttcagcat gttctcagtg gtgcggataa cgatcggcac
     5941 ctgcaacagc gccagcgcaa ttacgcccgc ccagccggag aagtgctcca tctgtgccac
     6001 cacgatggtg tagacaaaca gaccaaccac aatcgacggc gcggagagca gaatgtcgtt
     6061 aatgaagcga atcacttctg ccagccagga tttacgacca tattccgcca gataaatccc
     6121 cgccataatg cccagcggcg taccgaatac cgtggcccac aaaattaaca gcccgctacc
     6181 cgccagggcg ttcgccagac caccaccttc cgtattgggc ggcggcgtca tttcagtgaa
     6241 cagcgccagc gacataccgt cgataccgcg agtgatggtg gacattaaaa tccagatcag
     6301 ccagaacagg ccgaaggcca tcgtcgccat cgagagcgtc agcgcaatac ggtttttgag
     6361 gcggcgacgc gcctgcattt tgcggcgaga ttcagccagc gccgcagtgg tttgcatttc
     6421 aaccatagcc attagcgtgc cccctcattc ttagccaggc gcataatcat aaacttcgat
     6481 gcggcgagga cgatgaaggt aatcacaaac aggatcaggc ccagttccat cagtgcggca
     6541 acgtgcagac cggattccgc ttccgcaaat tcgttcgcca gcgcagaggt gatactattg
     6601 cccggcatgt acagcgaggc gctgtcgagc tggtaggtgt taccgatgat aaaggtcacc
     6661 gccatggttt caccgagcgc gcggcccagc cccagcatga tgccgccgat aacaccattt
     6721 ttggtgaacg gaagaacgat acgccagata acttcccagg tggtgcagcc aataccgtag
     6781 gccgactctt tcatcatcac cggggtttgt tcgaacacat cacgcattac cgccgcaatg
     6841 tacggaataa tcatgatggc gaggatcacg cctgccgcga ggataccgat accaaatgcg
     6901 gggccagaga acagcgcgcc aacaatcggg atattcgaca tgatattgcc gaccggctcc
     6961 tgaaagtaaa cggcgaacag cggcgcaaag ataaacaggc cccacatgcc gtaaacgata
     7021 cttggaatgg ctgccagcag ctcaatggcg atacccagcg ggcgtttcag ccagccaggc
     7081 gcaagctcag tcaggaacag ggcgataccg aaactcaccg ggacggcgat cagcagcgcg
     7141 ataaacgaag tcaccaacgt accgtagatc ggcaccagcg ccccgtagat atcgttcggt
     7201 gcatcccact ctttggtcca taggaaagcc agaccaaatt tctgaatgct cggccaggag
     7261 gagatgatca gagagacaat aatgccaccc aacatcaata gcacaatcag cgccgccagt
     7321 tttaccagca cgctgaaaat tatgtcgccc tttttacccg gtgggttaaa agcgggcttg
     7381 gttgcagcca taagttactc ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg
     7441 atgcggcgta aaacaccgta cccggcctgg agttttatta gtacagcggc ttaccgctac
     7501 tgtctttaat attggtcttc cacgcagcgc gaacctgttc aactacacta tccggcaggc
     7561 tggcgtaatc caggtcgttc gcctgtttag ccccggtttt gtacgcccag tcgaagaatt
     7621 tcagcacctc tgtgccttgt tctggtttct tctgatcttt gtggatcaga atgaacgtgg
     7681 tagaggtaat aggccatgca tcttcgcctt tctggttggt cagatcctga gcgaaggttt
     7741 tgctccagtc tgcacctttt gctgcattag cgaagttttc ttcggtcgga ctaaccggtt
     7801 taccatcagc ggagatcagt ttggtgtacg ccaggttgtt ctgcttcgcg taagcatatt
     7861 caacataacc aattgcaccc ggcagacgct gaacgaacgc ggcgataccg tcgttacctt
     7921 taccgcccag accgatcggc cattttacgg tagagccagt accaacgttg tttttccact
     7981 cttcgttcac tttcgccagg tagctggtga agacgaagga agtcccggag ccatctgcgc
     8041 ggcgtactac agcaatgttt tgtgaaggca gtttcagacc cggattcagt ttggcgatgg
     8101 cttcatcatc ccacttcttg attttgccca ggtagatgtc gccgagggtt ttaccatcca
     8161 gcaccagttc gccagacttc agccctggaa tgttaaccgc cagcaccacg ccgccaatca
     8221 cggtcgggaa ctggaacaga ccttcctgag ccagtttttc gtcagacagc ggcgcgtcag
     8281 aggcaccaaa atcaacggta ttagcgataa tctgttttac gccaccggaa gaaccgatac
     8341 cctggtagtt aactttatta ccggtttctt tctggtaagt gtcagcccat ttggcataca
     8401 ccggcgcagg gaaggttgca cctgcacctg tcaggcttgc ttctgcaaac acagagaaag
     8461 cactcatcga taaggtcgcg gcgacaacag ttgcgacggt ggtacgcata actttcataa
     8521 tgtctcctgg gaggattcat aaagcattgt ttgttggcta cgagaagcaa aataggacaa
     8581 acaggtgaca gttatatata aggaatatga catttttatg acagaaagga attaccaaat
     8641 aacttactga ttttaaatgt gaagatgaaa attccacatg ccatcttttt gtcatgatgc
     8701 atttaaaagt gataaagaaa tgggtgagat gacaacgatg tgatgaacaa tcgttgtcag
     8761 acctattagc gcacaatctc aacagttatc gtcgctgttg cagtaaattg ccccggggct
     8821 gggcgagctc cagtaaggct cgcaggagca gctgaaaaag ttacactacc tgatttttca
     8881 ccatacactg ttgatttatc catgtctgtt ggcaactcac caccgttcac tttgatttcc
     8941 ttattatttt tgtcgtaaat ttttatcccc acgttagagt ttgaagttac gaccattgaa
     9001 ttgtccgtat tgggttcggt agcataaaaa gacgtactga tatcatcg
//
LOCUS       DAJTRN010000139         8926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRN010000139 DAJTRN010000000
VERSION     DAJTRN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..86
                     /locus_tag="OO328_003580"
     CDS             <1..86
                     /locus_tag="OO328_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5932003.1"
                     /translation="YLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            76..249
                     /locus_tag="OO328_003581"
     CDS             76..249
                     /locus_tag="OO328_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5932004.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(298..1371)
                     /locus_tag="OO328_003582"
     CDS             complement(298..1371)
                     /locus_tag="OO328_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5932005.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1473..4184)
                     /gene="torS"
                     /locus_tag="OO328_003583"
                     /pseudo
     CDS             complement(1473..4184)
                     /gene="torS"
                     /locus_tag="OO328_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4267..5295
                     /gene="torT"
                     /locus_tag="OO328_003584"
     CDS             4267..5295
                     /gene="torT"
                     /locus_tag="OO328_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5932006.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5268..5960)
                     /gene="torR"
                     /locus_tag="OO328_003585"
     CDS             complement(5268..5960)
                     /gene="torR"
                     /locus_tag="OO328_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5932007.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6047..7219
                     /gene="torC"
                     /locus_tag="OO328_003586"
     CDS             6047..7219
                     /gene="torC"
                     /locus_tag="OO328_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5932008.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7219..8880
                     /gene="torA"
                     /locus_tag="OO328_003587"
                     /pseudo
     CDS             7219..8880
                     /gene="torA"
                     /locus_tag="OO328_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
BASE COUNT         2149 a         2313 c         2369 g         2095 t
ORIGIN      
        1 cttacctgac cagggtaccg ggaaccaaca ccttactggc gtgttgctgt ctttcaagac
       61 cagacgaggt taacagtgaa tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc
      121 gactttatcg cgcaaaaaat cgccgagctg aacaagaata cagggaaaga agtctctgaa
      181 attcgcttca tcgcacgaga aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa
      241 ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta
      301 gtgaccaatc attggcgctt gtggaattaa gcgtcggtac aattcctccg gcaccgggct
      361 ttgccatact cccgcataca ttgcgtaacc aatcaccgca aacataatcc ccagaaccag
      421 tagcgtcatt aaccagccag acaacgcaaa ggctttttta tttgctgcag gtttttgcag
      481 tgaaaaggtc aatgttgagg ctaccggaca tgactctacg caagtcatac agccggtaca
      541 ttccactgtt cgtacctgaa ttaatttatc taccgggatc cgtgatgggc acttttttgc
      601 gcatttgcca caatcgatac aactttcggc attgcgacga atcttaaacg gcgacaatag
      661 cgaaaccacg cccatcagcg cgccatatgg gcaaagataa cgacaccagg catggcgaat
      721 aaacaggctg gcaatcagca aaacggtcac gctgattaat gtcgcggtcc ccatatgacg
      781 aaagaaatcg agcattttaa cgtccatcac cacgctatag ggcgacaaca taaaatagtg
      841 aatcgcctga gcgggcatca ataacgcgat atagagaaat aaactcaaca gcaaatactt
      901 cacgccgcgc agaggaatat ccagccagcg gggaaggaca cattgccgac caaacagttt
      961 gttaccgaga tcgccgatta attcagaaag cgtaccaacc gggcataacc atgagcaaaa
     1021 ggcctttttg agtaatagac tgatgacgat aaaagcgacc aataacagca tcgcggcggc
     1081 gtggacggac ggtaactgac ctgttgcaag gctatatttc agattcatca gaccggcaat
     1141 cggtagccag ccttcgatac ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc
     1201 gtaatagcgc acccaatacc agaacgtgat ggcaatataa atattcattg ccaatagtaa
     1261 gaattgcgtc gctttacgcc aggtcgtggc attgcgccag tcattccacg gtaatttgcc
     1321 gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt ttctctgcca tgattttgcc
     1381 agtccgttaa gttgcatacc aaatgccact attctagtta ttcttaactg gctgatattg
     1441 attcaaatcg cgttcaggtc tttcttattc aatcatgctt ccagagcggc aacactgcgt
     1501 gtaatttctt cgtgtggaag gggggcagat aatggctgct gctccagttg tgcgcatagc
     1561 tgactggcgc tacgcattcc cagactcgag caactgcttt ttagctgatg cgcggcacgc
     1621 tttattttct cgctgtcctg gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg
     1681 gcatgttgtt taaataatgc cagccattcg tggatcttct ccgtccccat taactgagcg
     1741 tcttcattga gttgcgagac atccagtggt agatcgttat tggcttgcag ttggagatag
     1801 tgcgccagta actgaccgag cacttcacgc ggcaccggtt tagggataat cccgcgaaat
     1861 agtgaactgg tacgctggcg cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa
     1921 accagcgacg gatattgctg tgccagttgc cgggcaaggg taatgccgtc gatatccggc
     1981 agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc
     2041 tgcgcggcat tgccaatagc aacaacctgc gcaccactgg tgttcagcat ctcaacggta
     2101 attcgctggg ttagcgggtt atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc
     2161 gcctgattgg ctgtttttgg cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa
     2221 aaacagcttc caacctccgg cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc
     2281 agacggctac tgatagtcag ccccagcccg gtgccgccgc gtttgccgct tacctgcaca
     2341 aatggctgga agatttctgc cagtttcgcg ggatcaatac cgcagccgct gtcttccact
     2401 tcgaccagcc attgctcgcc atcagtgcga ctacgcagga taatgtgccc ttcgtcagta
     2461 aaacgcaggg cattgctcaa taggttggta atgacctgac gaatacgtcg tggatcgccc
     2521 attaacgcgc tcggtacatc atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc
     2581 acccgtccgc tcattaattg cagggtactt tccagcagcg ggcgcggttc aaagggctca
     2641 tcgctgactg aaacattttt gccacctgct tcgatagccg aataatcgag aatatcgttg
     2701 aggatggtca gcaacgattc gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg
     2761 ttaagtgcgg ggttatctgc cagcagttga gcagtgccga gaataccgta cagcggtgtg
     2821 cggatctcat ggctcatcgc cgtcagaaac gccgattttg cctggctggc tttttctgct
     2881 tccgcccgtg cctgtcggtg ttctatcacc agttcctgca attcagctgt acgcgctttg
     2941 acctgcgccg ccagctgttc gcggtggcga ttcagtgcat gaacactgct gcgaaacgca
     3001 tccatcagcc gcccgatggt atccagctcc cgtacgccag cggtttccgg gaaaggggag
     3061 tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg
     3121 aatgagcgat aaaccacgcg ccagaggatc ataatcagtg cgcaaagtga aaccatcccc
     3181 agcaataaca ggctgtattg cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc
     3241 tgattgtgca gctcaatggt gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac
     3301 tgggcgatat tattttgggc gagagtttgc aggtgattgc tgatttcact gtcctgctgg
     3361 aacagcgcca acagatcgct atattggcta acggtagtta acgttgttgc gacctgcgca
     3421 cgaactcccg gatcttcaat gcgtatttgc cgacgttgca gaattttcac cgcattattg
     3481 agctgctttt ccagcgttgg tgcatttttc tggatctgct ccagccccag attcatcacc
     3541 atttgctgca cccgcagagc gctaaggcgc agttcattca tctggttaac atactcaaga
     3601 tcgatatcaa tcagccgatc gagtgcactt tcagcagcct gacgctgatc ttgttcgatc
     3661 aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg tcgcattatt cgcctgacct
     3721 tgctccaggc gtgcgatctc atcggcggca gctatctgct ggctgagttg ccgttgttgc
     3781 tggcgtagtt gcagacgccg ccccaccagt tccccttgct gacgtaatga acgggagatc
     3841 tcctgctcct gttgttcaat agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc
     3901 aacgcattaa tcttcaggct ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt
     3961 tcgttatcgg cactggtcag gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc
     4021 aactggcgcg ccatattcat ggtaggaatc aatgcctgag tattgtcttt ttccacctgg
     4081 ctgataaagc gcaggttgta ccatcccacc agggtactgg tcagggttaa cagcgccatc
     4141 agggcaaagc ccatccagag tcttcgggtc agggttaaat tcacggtcgg tgcacttaag
     4201 gtgaaaaagg ttgagtcgca aagtggaatg catctagcat aaagccttat tattgatgag
     4261 gctatcatgc gcgtactgct atttttactt ctttcccttt tcatgttgtc ggcattttcg
     4321 gctgataacc tgttgcgctg gcatgatgcg cagcatttca cggtgcaagc ctctatgccg
     4381 cttaaagcca aacgcgtatg gaaactgtgc gcgctttatc ccagcctgaa agattcatac
     4441 tggttatcgt tgaactatgg tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa
     4501 gtgctggagg caggcggcta cagccagttg gctacccagc aagcacaaat cgaccagtgt
     4561 aaacagtggg gcgcagaggc cattttgctc ggtagtagca cgacgtcatt tcccgacctg
     4621 caaaagcagg tagcaaatct gccggtgatc gaactggtaa atgctcttga tgatccccag
     4681 gtgaaaagcc gcgttggtgt accctggttt cagatgggct atcaacctgg gcgatatttg
     4741 gtgcaatggg cgcacggtaa accactgaac gtgctgctga tgcccggacc cgataacgcc
     4801 gggggcagta aggagatggt cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt
     4861 attgttgata ttgcgcttgg tgataacgat attgaaatcc agcgcaacct gttgcaggag
     4921 atgctggaac gccacccaga aatcgacgtc gttgccggaa cggccattgc ggcagaggcg
     4981 gcaatgggtg aagggcgtaa cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca
     5041 catcaggtgt atcgcgggct gaagcgggga agagtgatta tggctgccag cgatcaaatg
     5101 gtctggcagg gggaactggc agttgagcag gccatcaggc aattacaggg gcaatcggtg
     5161 tctgataatg tcagcccacc gattttagtt ctgacgccga aaaatgccga ccgagaacat
     5221 atccgccgct cgctgtcacc ggggggattt cgtccggtct atttttatca gcacacatca
     5281 gcggctaaga aataaccttc accatgttgc gtcaccagta aatccgcgct gagtttatga
     5341 cgtaaacgac gaattaacac atcgacggtg cgcaggtcag ggttttccac ccgacgcgca
     5401 gaaagcatac gcagcagacg ctcacggctg agaatttcgc ccggattagt cacaaatgcc
     5461 accaacattt catactctgc gcgggtcagt ttaatcggct cgccatcccg ctccagcgta
     5521 tggcgcgaca cattcaggca ataacccgca aagcgatagc agtagtcctg agtgtgcggt
     5581 tgagcttgtc gcgcgaggtc gattcgccag agcagatttt tcacccgtac taccagttcg
     5641 cgcagttcca gcggtttggt gacgtaatcg tctgcgccca tttccagccc aacaatacgg
     5701 tcaatccgat cgctgcgtcc ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg
     5761 gcgcgggtta acatcaggcc attttcatcg ggtaagttga tatccagcag aattaaatct
     5821 accggctgat tctgcataat ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta
     5881 tacccctcct gagtgaagta ggattgtaat cgcgcctggg taaccggctt atcttcaaca
     5941 ataacaatgt gatgtggcat cagagggttt tactcattct gttcatatct gttcatattt
     6001 gctcattaag atcgcttcac taaaccataa ttctacaggg gttattatgc ggaaactctg
     6061 gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg caattgggat
     6121 tgtgattggc attgcgctga ttgtattgcc acacgttggg atcaaagtca ccagcacaac
     6181 cgaattttgt gtcagttgcc acagtatgca accggtgtat gaagaatata aacagtcggt
     6241 gcatttccag aacgcctccg gcgtgcgagc tgaatgccat gactgtcata tcccgccgga
     6301 tattccaggc atggtgaagc gcaaactgga agcgagcaac gacatttacc agacctttat
     6361 tgcccactcc attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg
     6421 tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgt cgctcctgcc ataactacga
     6481 tgcgatggat catgccaggc agcatcctga agcggcacgt cagatgaagg tggcagcgaa
     6541 agataatcaa tcctgcatcg actgtcataa aggtattgcc caccagttac cggatatgag
     6601 tagcggcttc cgtaagcagt tcgatgagct gcgcgccagt gctaatgaca gtggtgacac
     6661 gctgtactct attgatatta agccaattta tgcggcgaaa ggcgataaag aagcctctgg
     6721 ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat
     6781 tgaaattacc ggctggacgg aaagcgccgg acgtcagcgt gtactcaccc aattcccagg
     6841 taaacgcatc tttgttgcct cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga
     6901 gaaaaccacc gttgccgaca ccaataccga gtggagcaag ttgcaggcca ccgcgtggat
     6961 gaagaaaggc gacatggtga acgatatcaa accgatctgg gcctatgcgg attcgttgta
     7021 caacggcacc tgtaacgagt gccacggcgc accggaaatc gcccactttg acgctaacgg
     7081 ttggatcggc acgctcaacg gcatgattgg ctttaccagt ctcgataaac gtgaagaacg
     7141 cactttgttg aaatatctgc aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga
     7201 taagaaggaa gaaaaataat gaacaataac gatctctttc aggcatcacg tcggcgtttt
     7261 ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg
     7321 cgccgtgcaa ctgcggcgca agcggcgact gaggctgtca tctcgaaaga gggcattctt
     7381 accgggtcgc actggggggc tatccgcgcg atggtgaagg atggtcgctt tgtggcggca
     7441 aaaccgttcg aactggataa atatccgtcg aaaatgatcg ccggattgcc ggatcacgta
     7501 cacaacgcgg cacgtattcg ttatccgatg gtacgcgtgg actggctgcg taagcgccat
     7561 ctgagcgaca cctcccagcg cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc
     7621 ctcgacatgt tctatgaaga actggaacgc gtacagaaaa ctcacgggcc gagtgccttg
     7681 ctgaccgcca gtggttggca atcgacgggg atgttccata acgcttcggg gatgctggcg
     7741 aaagctattg ccttgcatgg taatagcgtt ggtacgggcg gagattactc taccggtgct
     7801 gcgcaggtga tcctgccgcg cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc
     7861 tggccgctgg tattgcagaa cagcaaaacc attgtgctgt ggggctccga tttgctgaaa
     7921 aaccagcaag cgaactggtg gtgcccggat cacgatgttt atgaatatta cgagcagcta
     7981 aaagcgaaag tcgccgccgg tgaagttgag gtcatcagca tcgatccggt tgtcacatcc
     8041 acccatgagt atctggggcg cgagcatgtg aagcacattg cggttaaccc gcaaactgac
     8101 gtgccgctgc aactggcgct ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac
     8161 ttcctcgcta actactgtgt gggttttgag cagttcctgc cgtatctgct gggtgagaaa
     8221 gacggtcagc cgaaagatgc cgcatgggct gaaaaactga ccggcattga tgccgaaacc
     8281 attcgtgggc tggcgcggca gatggcggcg aacagaacgc aaattattgc tggctggtgc
     8341 gtgcagcgta tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg
     8401 ctggggcaaa ttggcctgcc aggtggtggt tttggctggc actacaacgg cgcaggcacg
     8461 ccggggcgta aaggcgttat tctgagtggt ttctccggct ctacgtcgat tccgcctgtt
     8521 cacgacaaca gtgactacaa aggctacagc agcactattc cgattgcccg ttttatcgat
     8581 gcgatcctcg aaccggggaa agtgatcaac tggaacggta aatcggtaaa actgccgccg
     8641 ctgaaaatgt gtatttttgc cggaactaac ccattccatc gccatcagca gatcaaccgc
     8701 attattgaag gctggcgcaa gctggaaacg gttatcgcca tagataacca gtggacctca
     8761 acctgccgct ttgccgatat cgtgctgcct gcgaccacgc agtttgagcg taacgatctc
     8821 gaccagtacg gcaaccactc caaccgtggc attatcgcca tgaaacaggt ggtgccgccg
     8881 tagttcgagg cgcgcaacga ctttgatatt ttccgcgagc tgtgcc
//
LOCUS       DAJTRN010000140         8823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRN010000140 DAJTRN010000000
VERSION     DAJTRN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            40..735
                     /gene="minC"
                     /locus_tag="OO328_003588"
     CDS             40..735
                     /gene="minC"
                     /locus_tag="OO328_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5932009.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            759..1571
                     /gene="minD"
                     /locus_tag="OO328_003589"
     CDS             759..1571
                     /gene="minD"
                     /locus_tag="OO328_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5932010.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1575..1841
                     /gene="minE"
                     /locus_tag="OO328_003590"
     CDS             1575..1841
                     /gene="minE"
                     /locus_tag="OO328_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5932011.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2213..2557)
                     /locus_tag="OO328_003591"
                     /pseudo
     CDS             complement(2213..2557)
                     /locus_tag="OO328_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2592..2711)
                     /locus_tag="OO328_003592"
     CDS             complement(2592..2711)
                     /locus_tag="OO328_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5932012.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2672..2857)
                     /locus_tag="OO328_003593"
     CDS             complement(2672..2857)
                     /locus_tag="OO328_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932013.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            2958..3131
                     /locus_tag="OO328_003594"
     CDS             2958..3131
                     /locus_tag="OO328_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932014.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3133..3477
                     /locus_tag="OO328_003595"
     CDS             3133..3477
                     /locus_tag="OO328_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5932015.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3481..3816
                     /locus_tag="OO328_003596"
     CDS             3481..3816
                     /locus_tag="OO328_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5932016.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3873..6194)
                     /locus_tag="OO328_003597"
     CDS             complement(3873..6194)
                     /locus_tag="OO328_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5932017.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6311..6520)
                     /locus_tag="OO328_003598"
     CDS             complement(6311..6520)
                     /locus_tag="OO328_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932018.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7271..8794)
                     /locus_tag="OO328_003599"
     CDS             complement(7271..8794)
                     /locus_tag="OO328_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5932019.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2447 a         2064 c         1937 g         2375 t
ORIGIN      
        1 gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac tccaatcgag
       61 cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga acctaaggtt
      121 atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa acatgccccc
      181 gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat gcataaggcg
      241 gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc gcaacttaaa
      301 gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa agcgccacgt
      361 ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa aacgcgttta
      421 atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga tctgattgtt
      481 acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca tgtctatggc
      541 atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca aatattttgt
      601 acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag tgatcaaatc
      661 ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc tttgaccgtt
      721 caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt attgttgtta
      781 cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc actggtttgg
      841 cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt aatctcgacc
      901 tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt cagggcgatg
      961 caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat attcttccgg
     1021 catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa gttcttgatg
     1081 atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg attgaaaccg
     1141 gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac ccggaagtct
     1201 cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca cgccgcgcag
     1261 aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac ccaggccgcg
     1321 taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc atcaaactcg
     1381 tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt gaaccggtca
     1441 ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa cgtctgttgg
     1501 gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc aaacgcttgt
     1561 tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag aacacagcca
     1621 acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc gatgcagaac
     1681 cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa tacgtacaaa
     1741 ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt tctattcttg
     1801 agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt aaaaacgcat
     1861 ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat ccacgttgtg
     1921 gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc cccctttaca
     1981 ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt aaataaccat
     2041 gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt cattgttctc
     2101 ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga attttctctt
     2161 tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat ggtcagaacg
     2221 accagttcac accagccacc gcgttccacg gggactccac accggcacca tggctatacc
     2281 ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct gcctggtata
     2341 ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact ttcacccgat
     2401 tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt tccataccgt
     2461 tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata ctgcgggtat
     2521 cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt gacctaagct
     2581 ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag agacagcgca
     2641 ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata tcccgcataa
     2701 gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa atcactcttt
     2761 tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa aataaaatga
     2821 ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac aataatattg
     2881 ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca cttcttgaat
     2941 aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt cacgtaaaaa
     3001 acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat agcgagcgtt
     3061 ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact tacttaatac
     3121 cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc attgttctgc
     3181 tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct aggcgcaggc
     3241 gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc tgttggcgct
     3301 ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa aggtgcaaaa
     3361 gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct cgaaggggcg
     3421 attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat gaaataagga
     3481 atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc aatgacctgg
     3541 gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac ttgcgcagaa
     3601 gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc ggtaatgggt
     3661 aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact gagcgccaaa
     3721 gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt gatcacggca
     3781 attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac cgcgtcgatg
     3841 aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag gcccaccatc
     3901 actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc ccagacgctg
     3961 gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt actcacccca
     4021 tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt atggatccag
     4081 ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta
     4141 cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc attctcaata
     4201 cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat agcctgtgcc
     4261 tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt attcagggca
     4321 aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga ttcataggct
     4381 aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga gtcaatgtat
     4441 gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc gctatagcct
     4501 ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt gtagtcgcgg
     4561 gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc accggttaac
     4621 agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg tccgttgctg
     4681 tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga acgctcctgt
     4741 tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg cataacaaaa
     4801 agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg caggacgggg
     4861 gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc gctcgttgcc
     4921 tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg ggttaatgaa
     4981 tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt tgattccccc
     5041 tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc tgcattggta
     5101 actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc cgtagctgaa
     5161 ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac gtaattttca
     5221 gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat atagaccgta
     5281 ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca ggtactatta
     5341 tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc tgaacccgtt
     5401 atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat aaaaacatct
     5461 gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc tttatggtag
     5521 aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt tgcttcctga
     5581 ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc agatgtattt
     5641 ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg acccgttgta
     5701 taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc atgagcattg
     5761 tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt ataatcaagc
     5821 gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc tgttccgtgg
     5881 gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc attacttcct
     5941 gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct tccgggagat
     6001 gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt atcatctccg
     6061 tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt gtcagtattc
     6121 aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt gacaatcgtg
     6181 tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct agctgttaat
     6241 gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac aatgacgcca
     6301 ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt ccattacccg
     6361 ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt gccacagcat
     6421 gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc gccagagcaa
     6481 taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta tccattcatt
     6541 ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa acggagggta
     6601 aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata tataaatgat
     6661 aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac tttggggtga
     6721 ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc catggtaatt
     6781 atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta tttgcgcaat
     6841 aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag ccgcatactg
     6901 acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt tctcttaaat
     6961 cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc
     7021 acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt gatcagcata
     7081 aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact attattcatg
     7141 ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa
     7201 tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac
     7261 tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata attttgacca
     7321 ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt
     7381 ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa
     7441 gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttta
     7501 cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca tgaagataag
     7561 aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca
     7621 gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt
     7681 ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga
     7741 caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg
     7801 gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa
     7861 gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc
     7921 caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac
     7981 catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga
     8041 tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca gcggcgctgg
     8101 cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa
     8161 agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg
     8221 tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata atgtcatccg
     8281 ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg cgaatatgtt
     8341 gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag agcaggatag
     8401 gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc gggacaacag
     8461 gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact attccctgtt
     8521 tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca agctgatatt
     8581 gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc gcctgtgacg
     8641 cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc aggtactctg
     8701 cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata aacaagcata
     8761 tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca aagggtttaa
     8821 tac
//
LOCUS       DAJTRN010000141         8742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRN010000141 DAJTRN010000000
VERSION     DAJTRN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(864..2060)
                     /gene="hemY"
                     /locus_tag="OO328_003600"
     CDS             complement(864..2060)
                     /gene="hemY"
                     /locus_tag="OO328_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5932020.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2063..3280)
                     /gene="hemX"
                     /locus_tag="OO328_003601"
     CDS             complement(2063..3280)
                     /gene="hemX"
                     /locus_tag="OO328_003601"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5932021.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(3302..4042)
                     /gene="hemD"
                     /locus_tag="OO328_003602"
     CDS             complement(3302..4042)
                     /gene="hemD"
                     /locus_tag="OO328_003602"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5932022.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4039..4980)
                     /gene="hemC"
                     /locus_tag="OO328_003603"
     CDS             complement(4039..4980)
                     /gene="hemC"
                     /locus_tag="OO328_003603"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5932023.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5367..7913
                     /gene="cyaA"
                     /locus_tag="OO328_003604"
     CDS             5367..7913
                     /gene="cyaA"
                     /locus_tag="OO328_003604"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5932024.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7953..8273)
                     /gene="cyaY"
                     /locus_tag="OO328_003605"
     CDS             complement(7953..8273)
                     /gene="cyaY"
                     /locus_tag="OO328_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5932025.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8321..>8742)
                     /locus_tag="OO328_003606"
     CDS             complement(8321..>8742)
                     /locus_tag="OO328_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5932026.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1996 a         2252 c         2246 g         2248 t
ORIGIN      
        1 atctgttctc caatgacaag tctaaaaact agtattaaga ctatcactta tttaagtgat
       61 actggttgtc tggagattca gggggccagt ctagcagttg acggggtatc tgctgcgctg
      121 gccatgagag gaagagcggc ggcgacagca acaacaagac gtttgggtga aaacgaaaat
      181 tccatgcaaa atgctccggt ttcatgtcat caaaatgatg acgtaattaa gcattgataa
      241 ttgagatccc tctccctgac aggatgatta cataaataat agtgacaaaa ataaattatt
      301 tatttatcca gaaaatgaat tggaaaatca ggagagcgtt ttcaatccta cctctggcgc
      361 agttgatatg taaggcaggt ttattatatc gcgttgatta ttgatgctgt ttttagtttt
      421 aacggcaatt aatatatgtg ttattaattg aatgaatttt atcattcata ataagtatgt
      481 gtaggatcaa gctcaggtta aatattcact caggaagtta ttactcagga agcaaagagg
      541 attacagaat tatctcataa caagtgttaa gggatgttat ttcccgattc tctgtggcat
      601 aataaacgag tagatgctca ttccatctct tatgttcgcc ttagtgcctc ataaactccg
      661 gaatgacgca gagccgttta cggtgcttat cgtccactga cagatgtcgc ttatgcctca
      721 tcagacacca tggacacaac gttgagtgaa gcacccactt gttgtcatac agacctgttt
      781 taacgcctgc tccgtaataa gagcaggcgt ttttttatgc atcagaaagg tcccggaggt
      841 gctttcctcc gggtgagaag gaactactgt ggcgggttat tctgcaacgt taacatcaaa
      901 ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca gtctgtccag cgcgtcggca
      961 agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg ccgcgcggaa ggcgagcgat
     1021 gcttcctgcc attcaccgtg cttcatcagt gactgaccca atgtgctcca caacagcggg
     1081 cgatcaccga cgtttttgat ttgctggcgt agcacttttt ccagctgttc cgggttattg
     1141 gttttcagac gcgggatcgg cagcagtagg cgatcgtcat actggcgttt caggccgtcg
     1201 atgataattt gctgggcagt atcatgatcg tcacattcaa taagatgttc cgccattgcc
     1261 acctgcaacg ctacctgatg acgcgttttc cggctttggt ttttccacca gttacgcaaa
     1321 ccttcactac cgttatcggc acgcgcctga tccatcaggc caatccatgc ctgttgttcc
     1381 agcattgcac gatgttcttc atcaccaaca tgggctttcg ccattgatgg gatgatatcc
     1441 agcagagaac tccatgcccc tgtgcggata tacgcctgtt ccgccagacg taatacttca
     1501 ggatggcgtg gcgtaacttc cagcagctta tccacgccgt ggcgtgcagc atggttttca
     1561 ttacgggcca gttgcagacg tacacgggtg atttctaccg gaatggtgtc attgccggcc
     1621 agctccgctg cgcgttccag atgttggttg gcgcgtgctt catcaccacg ttgttgcgcg
     1681 gcttcggcag ccagtagata gttcaccacc ggttgttcag cgtgatcggc atttttcgcc
     1741 atcagctttt caacttgctg ataatcacct tccgccagtt tcagcagcgc ctgttcggtc
     1801 tgcttacgcg cacggcgacg cttacgtccg acaaaccatc cacgggtgtg cgcgccagtg
     1861 cggaagatcc gccgcagtag ccactcaatg gcaaacagca ctaccatcgc cagaatcaat
     1921 atgatcgcca ggcccgtgac gctggtttcg atattgtagt tgtcggtctg gatcagcaca
     1981 taaccctgat gaccggcaat catcgggcca accactatcc ccgcaatcag caaaacaaag
     2041 agtaataaca cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcacctt
     2101 gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtttagctt
     2161 ccgttgtccc cgctgccggt tgtgccagca ggttacgcac gcgagtctgc atcagttttt
     2221 ccagcatcgc ctggctttgc agggtttccg gaagatccat cgagatattt tgctggctta
     2281 actggtccac ctcgtcgagg aacgctttgg tggtggcatc atcagtatcg tagtaagcac
     2341 gtacccaggt ggagacgttc tccagcgcct ggcgataagt ctcttcctga tgacgcggta
     2401 cagcttgtgc tgcgaccagc aggcgagagc gaatattttc gcgcagatag atatcctgat
     2461 ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg aatcgtaatg aagttgtcca
     2521 taaagttctg ccagcttttt tgcagattga tacgccattc gctgatggaa ctggaaagct
     2581 cttcaccgtc tgaatccatc ggcgaaccat cgctgtcatt atcggccaga cgcaggttat
     2641 ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc gtcataatcc acctgcgata
     2701 ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg aacggtaatc agactcgggt
     2761 cattcatatc cgccaggctg gcgtctgcac ttttcagcaa cgctgcagag gtcgtgacgt
     2821 cctgatcgct ccacagcttc cgtccggcga gtttcaccag aaaatcggcc tgagccagca
     2881 gccaggtttt agcatcgctg ccggaaatgg tggcgatctt ttgttggact tcatccaact
     2941 gttttgccag cgtttcttgc tgacgattcg cctgcttaag ctgcgcagct tgttgcttaa
     3001 taatgccttc cagctcggct ttttggctct cctgggcttt ttgcaatgcc gtcagttggt
     3061 tagccagggc atcgctggtg gcggtctgat tgacggcctg ttgtttaccc cagccataca
     3121 aaccgacacc cgccgccaga gcaatagcga tagccaccgc gctgagaatc aatgcagtat
     3181 tgttcttact ctttttttct gttgcgacag gttgtgacgt ggtgtccacg gcctccctgg
     3241 tctcttcaac cacggcggag gttttttctt gttccgtcat tatggcttcc tgttatgaga
     3301 gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt atcggcgacc ttaatgtctt
     3361 gccagcccag ttcccgggcg agtttcgcca aacgctcact gacgaccaat agtcgacagt
     3421 gtagtaacca gtgctcacga taccattgtg ggatcagcga ccagagttgc tgcaacattt
     3481 caccgctggt aacaacgacc atcgtcacct cgcgggattg ccagcgcatc gcttcttctg
     3541 caccatcgta atggattgcg catcgttgat aacattcaca aaaagtgacc tcagcaccgc
     3601 gcaccgtcag ggtatcccca attagctcac gaccgccatt gccacgtaat atcagcgcac
     3661 gtttgcccgc aatattttgt aattcaggta attgtagcaa gacttcgctg atttcccgat
     3721 cctgtgggta gagaatcttc tgtccgctta cggtatgtag cgccagtgcg gtggtgcgtc
     3781 caatggcgaa ataagtaggt agttggggcc atttacgatc ttgctgatgc agctgtgatt
     3841 gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag atcgctctcc cccagcgctg
     3901 ccagttgatc agcaagttgc ggtaattgtc gacccggaga aaactcaatc agcggaaaat
     3961 gccaggccac ctgccccagt gtgcgcagac ggctcactaa ctcttctcca gcgggagacg
     4021 ggcgggtgac caggatactc atgccggggc ctctccgtta tagacttcag cgaggatctc
     4081 gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc cccatttgtt cggcatcttg
     4141 cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg tccggcgcgc cgaccagcgc
     4201 acatagccag atttcgccat caataagctc ggcgtagcta ccaattggca cctgacatcc
     4261 gccttcgaga cgggtattca tggcgcgttc tgcggtaacg cgcagtgcag tttcgtggtg
     4321 attcagcgcg gcaagcagct cgcgagtgcg tgtatcatca aggcggcatt caataccgac
     4381 tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt aacgcggcgc gaatgcggga
     4441 ctccagacct aaacgtttta gtccggctac ggcaagaatg atggcatcgt attcgccgtt
     4501 atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg gagcggataa tcagatccgg
     4561 gcggcgttca gccagttggc actggcgacg taaactggac gtcccgacga tactgcctgc
     4621 tggtaacgca tccagactgt catagttatt ggacacaaag gcatcgcgag gatcttcacg
     4681 ctcgcaaata gtgaccagcc ccagaccttg cgggaattca accggcacat ctttcattga
     4741 atgtacggcg atatcagcgc gattctccag gagcgcgact tccagctctt ttacaaataa
     4801 gccttttccg cctactttcg ccagcggcgt atcaagaatc acatcgccgc gcgtcaccat
     4861 cggtaccagt tcaacgacca ggcccggatg gctcgccatc aacttgtctt tgacatagtg
     4921 tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt cttaaaacat tgtctaacat
     4981 gcttgttacc gtcattatca tccgtggtcc atcctaacat ccttgccaga gtgatgtcag
     5041 tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat gaggtctggc agtggatcct
     5101 gacaggcgtt tcacgccgtc gtaataagga atttacagag aataaacggt gctacacttg
     5161 tatgtagcgc atctttcttt acggtcaatc agcaaggtgt taaattgatc acgttttaga
     5221 ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa agtggtaacg gttacctttg
     5281 acatacgaaa tatcccgaat gccgcgtgtt accgttgatg ttggcggaat cacagtcatg
     5341 acgggtagca aatcaggcga tacgtcttgt acctctatat tgagactctg aaacagagac
     5401 tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc tgctatgggg cctgcattcc
     5461 aacaggtcta cagtctactg ccgacattgt tgcactatca ccatccgcga atgccgggtt
     5521 accttgatgg taacgttccc aaaggcattt gcctttacac gcctgatgaa actcaacgcc
     5581 actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt acaggatcca ccgaaaggtg
     5641 agcttccaat tactggtgta tacaccatgg gcagcacctc gtccgtaggg caaagttgtt
     5701 cctctgacct ggatatctgg gtctgtcatc aatcctggct cgatagcgaa gagcgccagt
     5761 tactacaacg taaatgcagc ctgctggaaa gctgggccgc ctcgctgggt gtggaagtca
     5821 gcttcttcct gattgatgaa aaccgcttcc gtcataatga aagcggcagc ctggggggcg
     5881 aagattgtgg ctccacccag catatactgc tgcttgacga attttatcgt actgccgtgc
     5941 gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg cgacgaagaa gagcattacg
     6001 acgactatgt gatgacgctt tacgcgcagg gcgtgctgac gccaaatgaa tggctggatc
     6061 tcggtggctt aagctcgctt tctgctgaag agtactttgg tgccagcctt tggcagctct
     6121 acaagagtat cgattcccca tacaaagcgg tgctgaaaac actgctgctg gaagcctatt
     6181 cctgggaata cccgaaccca cgtctgctgg cgaaagatat caaacagcgt ttgcacgacg
     6241 gcgagattgt atcgtttggc ctcgatccat actgcatgat gctggagcgt gttactgaat
     6301 acctgacggc gattgaagat ttcacccgtc tggatttagt acgtcgctgc ttctatttaa
     6361 aagtgtgcga aaaactcagc cgtgaacgcg cctgtgtagg ctggcgtcgc gcagtgttga
     6421 gccagttagt gagcgagtgg ggttgggacg aagctcgtct ggcaatgctc gataaccgtg
     6481 ctaactggaa gattgatcag gtgcgtgagg cgcacaacga gctgctcgac gcgatgatgc
     6541 agagctaccg taatctgatc cgctttgcgc gtcgcaataa ccttagcgtc tccgccagtc
     6601 cgcaggatat cggcgtactg acgcgtaaac tgtatgccgc gtttgaagca ttgccaggta
     6661 aagtgacgct ggtaaacccg cagatttcac ccgatctctc ggaaccgaat ctgaccttta
     6721 tttatgtgcc gccgggccgg gctaaccgtt caggttggta tctgtataac cgcgcgccaa
     6781 atattgagtc gatcatcagc catcagccgc tggaatataa ccgttacctg aataaactgg
     6841 tggcgtgggc atggtttaac ggcctgctga cctcgcgcac ccgtttgtat attaaaggta
     6901 acggcattgt cgatttgcct aagttgcagg agatggtcgc cgacgtgtcg caccatttcc
     6961 cgctgcgctt acctgcaccg acaccgaagg cgctctacag cccgtgtgag attcgccatc
     7021 tggcgattat cgttaacctg gaatatgacc cgacagctgc gttccgcaat caggtggtgc
     7081 attttgattt ccgtaagctg gatgtcttca gctttggcga gaatcaaaat tgcctggtag
     7141 gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt gcgtacgctg cacttcaacg
     7201 gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg caaaatgcat caggacgccg
     7261 caccgccaga tagcgtggaa gtcttctgtt atagccagca tctgcgcggc ttaatccgta
     7321 ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg cctttccagc acccgccagg
     7381 aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac ctggggcttg ttcttcgaac
     7441 gcctgaatgt atcggtacag aagctggaga acgccatcga gttttatggc gctatttcgc
     7501 ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa tcacgtcaaa ttaccggcgg
     7561 tggtggacgg ctttgccagc gaagggatca tccagttctt tttcgaagaa acgcaagacg
     7621 agaatggctt taatatctac attctcgacg aaagcaaccg ggttgaggtg tatcaccact
     7681 gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg cttctactcg tcatcgcacg
     7741 accgcttcac ctacggctca agcttcatca acttcaacct gccgcagttc tatcagattg
     7801 tgaaggttga tggtcgcgaa caggtgattc cgttccgaac aaaatcgatt ggcaacctgc
     7861 cgcccgccaa tcaggatcac gatacgccgc tattacagca gtatttttcg tgatgagcgt
     7921 gccggaaagc gaggcttatc cggcatgcaa tcttagcgga aactgactgt ttcacccgcc
     7981 tgttgcgtcg ccgcctgttc cagcaaatcc cagaaggttt cgccgctgcg atcgcaaatc
     8041 cattcatcgc ctttcaggtc aaaatggtag ccgccctgtt tggttgccag ccacacctgg
     8101 tgcagcggct cctggcggtt aataatgatt ttgctgccgt tttcaaaggt aatggtcagt
     8161 acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat ccctgtcgtc cagacgctct
     8221 tcaatggtca gccacagttg atcagccagg cgatgaaatt cactgtcgtt cattgttgta
     8281 tcctgttttt aagtgatggc ggcagtatag cggcatgggg tcagggcttc aaagtttgca
     8341 cctctgctgc tgcgttccgg catgattcat ccgtcaccgg aataatgatg tctctgtttt
     8401 gaacttcacg tacgttctcc tctctgacac gagttttcgc cgggcagggg gagggcgtaa
     8461 tccttacctc tgtatcggtc catgtataaa cattgcgatc gtcataagcg aagcgatcgt
     8521 ttttagttag cgaaccgtta actggaataa tttgcaagct ctttgaccgt aattggcgga
     8581 taaagtaagc atgctgtttg ccgctattaa atgagcgatc aatgaccagg tctggcgtat
     8641 ccagcgggat tatctcaagc tggtcttcac caaatgtcga taattttgtt acatacagcc
     8701 gatctttata ttgcgcaata ttgccagtaa tcagatggaa tt
//
LOCUS       DAJTRN010000142         8719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRN010000142 DAJTRN010000000
VERSION     DAJTRN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..297
                     /locus_tag="OO328_003607"
     CDS             <1..297
                     /locus_tag="OO328_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5932027.1"
                     /translation="HGPAAFLALRHSDNPLNGYSICAFPDAADKQTPDIGYMPGHLTW
                     YFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(405..1763)
                     /gene="hprS"
                     /locus_tag="OO328_003608"
     CDS             complement(405..1763)
                     /gene="hprS"
                     /locus_tag="OO328_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5932028.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(1763..2434)
                     /gene="hprR"
                     /locus_tag="OO328_003609"
     CDS             complement(1763..2434)
                     /gene="hprR"
                     /locus_tag="OO328_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5932029.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2567..2980
                     /gene="hiuH"
                     /locus_tag="OO328_003610"
     CDS             2567..2980
                     /gene="hiuH"
                     /locus_tag="OO328_003610"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5932030.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3089..4093
                     /gene="msrP"
                     /locus_tag="OO328_003611"
                     /pseudo
     CDS             3089..4093
                     /gene="msrP"
                     /locus_tag="OO328_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4094..4729
                     /gene="msrQ"
                     /locus_tag="OO328_003612"
     CDS             4094..4729
                     /gene="msrQ"
                     /locus_tag="OO328_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5932031.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4987..5637
                     /gene="zinT"
                     /locus_tag="OO328_003613"
     CDS             4987..5637
                     /gene="zinT"
                     /locus_tag="OO328_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5932032.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(6715..6984)
                     /locus_tag="OO328_003614"
     CDS             complement(6715..6984)
                     /locus_tag="OO328_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5932033.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7041..7709
                     /locus_tag="OO328_003615"
     CDS             7041..7709
                     /locus_tag="OO328_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5932034.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8025..>8719
                     /locus_tag="OO328_003616"
     CDS             8025..>8719
                     /locus_tag="OO328_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5932035.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2512 a         1957 c         1769 g         2481 t
ORIGIN      
        1 cacggtccgg cggcttttct ggcacttcgc cacagcgata acccactgaa tggttattct
       61 atttgcgcat tcccagacgc cgcagacaaa caaacgccag atattggcta tatgccgggt
      121 catctcacct ggtacttcgg cgaagagttg aagaaaatgg gcatgaacat cattaatgac
      181 gacatcaccg ggcgagtaca taaggaccgt aaacgtctca ccggcgacag tccttttgca
      241 gcgaatgcgt tgggtaaact ggcggcgcag gaaatgctgg cagcttacgc gagttaatcc
      301 ctgttacatt gtgatgtaag cgcataatca atcgcagcca cgttaatact cacgacgtgg
      361 ctgcgttttt tcacggttaa cttatggcgt actgaagccc gatgttaatt tctttgcggt
      421 aacgtaattc ggaacacgtt atgcttactg agatagtgat acgtagcact tcctccatgt
      481 aattcggcaa tcgctttgac taaagaaagg ccaagtccct gacctacgga atgacgcgaa
      541 ttatctcccc gccaaaatct acggaagagt ttttcaggct cattaatttt cgttccagga
      601 ctggcgatat caatattaag agagccgttg gcatcaagaa aactggttat atgaatacgc
      661 gatttttctg gtgaatatcg aatggcgtta acaataagat tcgataacat tcgttgcagt
      721 aaaattttat ccgcaaagat ttgttgattg cacttgacct taaagcaaat ctctttctcg
      781 tctgaaagat attcaagata gtccaaaaga ttttcaactt ccttattgag agaaagcgaa
      841 tccagtttca ccaaaacatt gtttttgtct gccctggcaa gaaacagtat attctctgtt
      901 aaccgcgaaa tattttccag ctcttcaatg tttccggcaa ttgttttttg atattcagcg
      961 atacttctga tttgactgag cgtaacctga ttctgaccca gtaatgcatt aattggcgtt
     1021 ctaagttcat gagcgagatc gtcagcaaat tgactaaggc gctcaaaatc tttgactaaa
     1081 gcatgatgca ttttattcaa cgcctgccca agaggtttta gttctctcgg taatgcgtta
     1141 acctcaaccg gcactcggct atcgttataa ttcagcgctt ccgttacgcc actcaacttt
     1201 ttgatctctc gcaaccccgt cctgattaac aacggactta atactgagca cagtataatg
     1261 gcgacaatgc aaattataat gctattaatt ttatactgtt caagcatatt atgtctggct
     1321 gaagccaatt tagccacagt aaccgttaat gatggcgaaa cttcatcaat gttaattcgt
     1381 aaagcatcta tctctgtatc attaataatg cttctgtaaa tgcctgctgc gctgattgtc
     1441 tcactagcag gtatgttatt taacatgtca tcactgacat ttgtccggtt aacaatttta
     1501 ttgatgccat cactatgaat gatcaaaata tcctgactaa catccatcat ccggttaaag
     1561 tacacaggta acgtatctgg atttacccca tcaattaaca actgcttgat ctgcgctgtc
     1621 cggttaatga gtgttgtatc atcgcgccat ttcaactcac ttgccaggcc attatagaga
     1681 gtccatacaa ttccagcgcc agcaacagac tgcagaaata taaaaagcaa ggttaaacgg
     1741 acggttatag ataatctttt cattattttt ttaccgctac gaatgaatag cccatccccc
     1801 ggattgtggc gattagcttt tcaggaaaag gatcatcaac ttttgcgcgg agcctgcgaa
     1861 tggcgacatc caccgtattg gtatcactat caaagttgat tccccaaatt tcactcgcaa
     1921 taaccgttct gggtataatc tcgccagctc tggaggccag tagccaaagt aactgaaact
     1981 ccttgcgcgt cagtgtaata ctgatattgt ccctgctgac actttgactg acagagtcca
     2041 tttttaagcc actgatttct aatgttgaat tcagagcgtg atgttgcctt aattgtgccc
     2101 gaacccttgc tagcaactca gaaaatgaaa aaggttttac cagataatca tttgccccac
     2161 tgtccaaccc tctgactctg tcatcgacag aatcccttgc agtaaggcaa ataacagggg
     2221 tttgctttgc cgttcttaac gtttgtaaga tctgccagcc atccataccc ggaagcataa
     2281 tatccagaat gatcaatgca taatcatcct tcagcgcaag ataaagccca tctctgccat
     2341 cagaaacggc atcaatgaca taacccgctt cggaaagccc ctgcgttacc cattcctggg
     2401 tcctttgatt atcttcaata agtagaatct tcatgaaaat aatatgccat aaatcatatg
     2461 ttatagaaac aacctggttc attacaaaat tgtaatgctg ctgtaaggtt accctggacg
     2521 ttttttcgct atcctcaaaa ctcattcaca tgacaaggat ataaacatgt taaagcgtta
     2581 tttagtactc tcggtagcaa cggcagcctt ttcattacct tctttggttt atgccgcaca
     2641 acaaaacatt cttagcgtgc acattttgaa ccagcaaaca ggaaaacccg ctgccgacgt
     2701 gacagtcact cttgaaaaga aagcggataa cggctggtta caacttaata ccgccaaaac
     2761 agataaggat ggacgaatta aggcactgtg gcccgagcaa actgcgacta ctggcgatta
     2821 ccgtgtcgta tttaaaaccg gggactattt caagaaacaa aatcttgaaa gtttcttccc
     2881 tgagattccc gttgaatttc atattaataa agtgaacgag cattatcatg tgcctttgtt
     2941 actcagccaa tatgggtatt caacctatcg tggcagttaa tgcgtaaata tgtaaaagct
     3001 gatctctgct actgttaggg aggtgactcc ctgacagatt aacagtaaac agctcttgtt
     3061 ggttaacgac aaaaaagtgt gatggcttat gaaaaagaag caatttttaa aagaatcaga
     3121 tgttacggcc gagtcggtat tctttatgaa gcgtcggcag gtgttaaaag cactgggcat
     3181 cagcgcagct gcattttctt tgcctcacgc tgcgcatgcc gatctgctta gctggtttaa
     3241 agggaacgat cgtccgcccg cccccgccgg aaaaccgctg gagttcagca agcctgccgc
     3301 ctagcaaaat aacctgccac tgacgccagc agataaagtc tccggttata acaacttcta
     3361 tgaattcggg ctggataaag acgatcccgc ggctaatgct ggtagcctga aaaccgatcc
     3421 atggacactg aaaatcagcg gcgaagtggc aaaaccattg accctcgatc atgatgattt
     3481 aacccgtcgc ttcccgctgg aagagcgtat ttatcgtatg cgctgcgtgg aagcgtggtc
     3541 gatggtggtg ccgtggattg gttttccgct acacaaattg ctggcgcttg ccgaacccac
     3601 cagcaatgcg aagtatgtcg ctttcgaaac gatttatgcg ccggaacaga tgcctggcca
     3661 gcaggaccgc tttatcggcg gcgggctgaa atatccttat gtcgaaggat tgcgtctcga
     3721 cgaagcaatg catccgctca cactgatgac cgtaggtgtt tatggcaagg cgttaccacc
     3781 gcaaaatggc gcgccggtgc gactgattgt gccgtggaaa tatggcttta aagggattaa
     3841 atcgatcgtc agtattaagc tgacccgcga gcgtccgcca accacctgga atctggcagc
     3901 gcctgacgaa tacggttttt acgccaacgt taatccgcat gttgatcacc cgcgctggtc
     3961 acaggctacc gaacgattta ttggttcagg cggcattctc gatgttcagc gccagccaac
     4021 actactgttt aatggttacg ccgaccaggt ggcatcgctg tatcgtggcc tggatttgcg
     4081 ggagaatttc taaatgcgtc tgacagcaaa acaagtgata tggctgaaag tttgcctgca
     4141 tcttgccgga ttgttgccgt ttctctggct ggtctgggcg atcaatcacg gtggactggg
     4201 tgccgatccg gtgaaagata ttcagcattt taccggtcgc actgcactga aatttttgct
     4261 tgcggccttg ttaatcacac ctctggcacg ctacgcaaaa cagccgttat tgatacgcac
     4321 tcgccgcctg ttaggcttat ggtgctttgc ctgggcgaca ctgcatttaa ccagttacgc
     4381 attgctggag ttgggcgtga ataatctggc gttattaggt aaggagttaa ttacccggcc
     4441 ttatttaacg ttaggaatta tcagctgggt tattttgctt gctttagcgt tcacttcaac
     4501 ccagtcgatg cagcgaaaac tcggcaaaca ctggcaacag ttgcataact tcgtctatct
     4561 tgtcgcgatc ctggccccaa tacattatct gtggtcagtg aagattattt caccgcagcc
     4621 actcatctac gccgggctgg ctgtactgct tttagcctta cggtataaga agttgctttc
     4681 ccttttcaac cggttacgca agcaggccca caacaaatta tctctataac ttcatattgc
     4741 cgacaaactt cgcgttatcc cggccggatg cggcgcgagc gccctatccg gcccacacag
     4801 atcgcatcaa gttatgtttc gtttgtcatc aacctttcat caccagaata atttatttta
     4861 tttcaataag atagttcaat acacctgaga ataccatgct ctcatttcct aatgagttgt
     4921 tgcatttttt catatgttac aatataacat tacacataat gtacattaac tctggaggaa
     4981 attgttttgg cgattcgtct tcataaactg gctgttgctt taggtgtctt tattgttagc
     5041 gctcctgcct tttcgcatgg tcatcattct catggcaaac ccttaacaga ggtcgaacaa
     5101 aaagctgcta atggtgtttt tgatgatacc aatgtacaaa accgaacgct cagtgactgg
     5161 gatggagtct ggcaatccgt ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt
     5221 cagaagaaag cggatgcaga taaaaccaaa acatttgctg aaattaaaga ttattatcgc
     5281 aaaggttatc cgacagatat cgaaatgatt ggcattgagg acggcattgt cgaattccat
     5341 agaaataatg aaacaacatc ctgtaaatat gattacgatg gatacaaaat actcacctat
     5401 aaatcaggca agaaaggcgt tcgttactta ttcgaatgta aagatcctga aagcaaagcc
     5461 cctaaatata tacaatttag cgatcatata attgcaccac gaaaatcatc tcattttcac
     5521 atttttatgg gtaatgattc ccagcaatct ttattgaatg aaatggaaaa ctggccaacg
     5581 tattatccct atcagttgag tagcgaagaa gtggtcgagg agatgatgtc tcattgattt
     5641 ttttgcgctg acttcagtta tttaacagaa cagtgcctgg tagtaacaac aataatttac
     5701 caagacaaat aagtaagaca aactcagaca cgctcatact tgagtcgtgt cttttttatt
     5761 atgtctgtag aaaaaaactg agaaaaaggc taacaataaa tacacaactc agccatacag
     5821 actggcagcg caaatgtttc caggagcaag tcatctgtca tggtaaagct aaccagaaat
     5881 ataggttaat tttggtgttt aaaccttttt tacttgttag tatgatacaa aggctttcaa
     5941 aaaagctggg aaattcaaca actcttggaa attatggact ttgtcccgcg gctttaatct
     6001 gacggccgcg ttcctttttt ggctcaatta ttgacccctt ctctacgcaa cttcagttcc
     6061 cgccagcaac tttgcggcag tttcatagga tcaatgtcta aaagaataat ggtgaccgat
     6121 aagaaaacga ctgaataact gtagattttc gctcgaaacc ttcctgtcag ctccatagcg
     6181 aatcaagcgc tgaatgtcac agtatcgaac agaaaacagt gacgatctaa cccttcaaga
     6241 atattctacg attgttctgt ttaggaaaag caaggcggga agtcgggaga taagtcattg
     6301 ataaaatggc ggagagaggg ggatttgaac ccccggtaga gttgccccta ctccggtttt
     6361 cgagaccgat gatacagagt cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg
     6421 tcttaaaatc gtacacgaaa aatcaatgaa ttacataaca ttaaagaaga atattccaag
     6481 ccttcttgag tccaccacct gttttcaggt gtcattcaga cgtgatttgc cccagattgg
     6541 gaaagcacgc ttttttctca gcttattccg ttttgcctgt gtgttgaata attccactgc
     6601 agttatataa aatcggtaac ggctggaaat cattcaatac tcgcactatc gaaagtttac
     6661 cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat
     6721 tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag
     6781 caacacccga tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc
     6841 cagatctaca gcatcctgaa gtggcgcaat atgctcactg aatccctgga tgtagaactg
     6901 tgtggtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc
     6961 aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa
     7021 ttcacaaata aaactataat atggtgaaat taatgaaaaa aaacacagat gatggggcta
     7081 aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc
     7141 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
     7201 gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga
     7261 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
     7321 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcctgcgg
     7381 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
     7441 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa
     7501 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
     7561 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
     7621 taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag
     7681 taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac
     7741 gtgctacggt ttcctttacg atttggggtt gcgactttac ctctattgat aatgcattcc
     7801 ggcagaacgt tcaaatatga acgtacgata tttaactaac cgaaaaacaa aatatagcac
     7861 aggcgagaca tttaccatct aagaaaatag tatcgttttt ttacagttaa atcaacatca
     7921 cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gcttattatt
     7981 ccccagtatc acattttatc aggatatcct tctgcgaggt tattatgctt cctgtaaata
     8041 atccccccct atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc
     8101 acaataatta tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca
     8161 gggaaactgc ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact
     8221 tatcttgttt aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg
     8281 taagcaataa ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg
     8341 caagcagtaa tcaattatct gcacttcctg aattaccagt gtcgctggaa tatataaatg
     8401 tgagtgataa ccatttgttc gcacttcctg aattacctgc gtcactagaa tatattaatg
     8461 taagtgacaa tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg
     8521 cagccagaaa tgctttggaa gtaataccag attttccaga aagagatgat catattataa
     8581 gaatattctg gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca
     8641 gttctgatag cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt
     8701 tgttacaaca aaccgccca
//
LOCUS       DAJTRN010000143         8668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRN010000143 DAJTRN010000000
VERSION     DAJTRN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..376
                     /locus_tag="OO328_003617"
     CDS             <1..376
                     /locus_tag="OO328_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5932036.1"
                     /translation="YDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFISMALFC
                     VHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSFNLALV
                     IAACVTFTGALCYFFIVKDRIE"
     gene            628..1545
                     /gene="oxyR"
                     /locus_tag="OO328_003618"
     CDS             628..1545
                     /gene="oxyR"
                     /locus_tag="OO328_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5932037.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1528..2928)
                     /gene="sthA"
                     /locus_tag="OO328_003619"
     CDS             complement(1528..2928)
                     /gene="sthA"
                     /locus_tag="OO328_003619"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5932038.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3249..3899
                     /gene="fabR"
                     /locus_tag="OO328_003620"
     CDS             3249..3899
                     /gene="fabR"
                     /locus_tag="OO328_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5932039.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3899..4258
                     /locus_tag="OO328_003621"
     CDS             3899..4258
                     /locus_tag="OO328_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5932040.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4298..5398)
                     /gene="trmA"
                     /locus_tag="OO328_003622"
     CDS             complement(4298..5398)
                     /gene="trmA"
                     /locus_tag="OO328_003622"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5932041.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5512..5702
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5767..7611
                     /gene="btuB"
                     /locus_tag="OO328_003623"
     CDS             5767..7611
                     /gene="btuB"
                     /locus_tag="OO328_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5932042.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7556..8413
                     /gene="murI"
                     /locus_tag="OO328_003624"
     CDS             7556..8413
                     /gene="murI"
                     /locus_tag="OO328_003624"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5932043.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2008 a         2105 c         2309 g         2246 t
ORIGIN      
        1 ctacgacctg gcgaagacgc gtaaaacggc tattgtctgc ggtttgatga tgtcggcatt
       61 aggcacgttg ctggtcgtgc aatcttcctc gccagctcag gcggtagcgt ttatctcaat
      121 ggcgctgttc tgcgtgcatt tcgcgggaac gtctgcatgg ggactggtgc aggtgatggt
      181 gtcagaaaca aaagtggctt ccatcgccgg gattcaaaac tttggcagtt ttgtttttgc
      241 ttcctttgct ccgatcgtaa ccggttgggt agtggatacc acacactcgt ttaatctggc
      301 gctggttatc gcggcctgtg tgacgttcac cggggcgctg tgttacttct ttattgtcaa
      361 agatcgcatt gagtaatcgt tactatcagg ctctcttgct gtgggcctgt agaataaaaa
      421 aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg agttgagaaa
      481 ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt tctgtgagca
      541 attatcagtc agaatgcttg atagggataa tcgttcattg ctattctacc tatcgccatg
      601 aactatcgtg gcgatggagg atggataatg aatattcgtg atcttgagta cctggtggca
      661 ttggctgaac accgccattt tcggcgtgcg gcagattcct gccacgttag ccagccgacg
      721 cttagcggcc aaattcgtaa gctggaagat gagctgggcg tgatgttgct ggagcggacc
      781 agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc gcgtaccgtg
      841 ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggtgagac gatgtccgga
      901 ccgctgcata ttggtttgat tcctacggtt ggaccatacc tgctaccgca tattatcccg
      961 atgctgcacc agacatttcc aaagctggaa atgtatctgc atgaagcaca gacccaccag
     1021 ttactggcgc aactggacag cggcaaactc gattgcgtga tcctcgcgct ggtgaaagag
     1081 agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc tatctatgaa
     1141 gatcacccgt gggcgaaccg cgaatgcgta ctgatggccg atctggcagg ggaaaaactg
     1201 ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg ctttgaagcc
     1261 ggggcggatg aagatacaca cttccgcgcg accagcctgg aaactctgcg caacatggtg
     1321 gcggcaggta gcgggatcac tttattgcca gcgctggctg tgccgccgga gcgcaaacgc
     1381 gatggggttg tttatctgcc atgcattaag ccggaaccat gccgcactat tggcctggtt
     1441 tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc catccgcgca
     1501 agaatggatg gccatttcga taaagtgtta aaacaggcgg tttaaaccgt ttaacgcagc
     1561 tacccgatag gcttccgcca tcgtcgggta gttaaaggtg gtgttgacga agtactcaat
     1621 agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa tttcggcagc
     1681 gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga acaaaatttt
     1741 cagcgtgccc acgttcatgc cgacgatttg tgcacgtgcc agatgtttga actgggcgcg
     1801 gcccacttca tacggcactt tcatcgcggt cagctgctgt tcggttttgc ccacagagct
     1861 gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgtg cggtggcttc
     1921 gccttttacc agcgcctgcg cggcaatgcg cccctggtca taggccgctg atgccaggct
     1981 cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct gatacatgct
     2041 gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta acgccagcga
     2101 atcggtatta ccggtacgac cgttggcata gagcaggcag tcagctttca gttttttacc
     2161 cgacttcaga tgcatgatca caccgtcgtc acagctttcg atcttctcgt actcttcgtt
     2221 gtgacgaatc actacgccac tgttccagaa gtggtaggag agagaatctg acatctcttg
     2281 atcgagaaat gccagcaggc gatcgcgggt gttgatcaga tccactttta catccatacc
     2341 gcggaagatc gacgcatatt cacagccgat cactccagca ccatagataa gtacatggcg
     2401 cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa atgcgtggat gggtgaaatc
     2461 aacatctgtt ggatgatatg gacgagagcc gcaggcaata acaaattttt cagcggttag
     2521 tgtttcaacg ctgccgtccg ggcaatccag cgccaacgta tgctcgtcaa caaagcgagc
     2581 gttcccctgc aatatttcac agtgattacg ttcgtaaaat ccctgacgca tgcgcgtttg
     2641 ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc ggagcagtcg
     2701 ggaatggtcg ctgtatagtg ggttttgatt gaattctata atgcggctga cggcgtgacg
     2761 gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat tttgataacg
     2821 ctcgataact gcgacgcgcg caccttgctt aaccaggccc attgcagcgc cttcgccgcc
     2881 ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg tagggcttac
     2941 ctgttcttat acataaaagc aacagaatgg taacattcta tcgcgggtaa gccaattgat
     3001 ccccgtcatt tatctggcta tatcctgagc ggcctttgct ttgtctgttt cttacttttg
     3061 ccctgacgtt ttattggatt tttatcgacg atactctccg tttaagcggc aggtttccgc
     3121 tgtacgtaaa agaaccggcc agtaaatatg ttttattgcg ttaccgttca ttcacaatac
     3181 tggagcaatc cagtatgttc attctctggt atagtgccag cagtactttt ggcaaggatt
     3241 cagacatcgt gatgggcgta agagcgcaac aaaaagaaaa aacccgccgt tcgctggtgg
     3301 aagccgcatt tagccaatta agtgctgaac gcagcttcgc cagcctgagt ttgcgtgaag
     3361 tggcgcgtga agcgggcatt gctcccacct ctttttatcg gcatttccgc gacgtagacg
     3421 aactgggtct gaccatggtt gatgagagcg gtttaatgct acgccaactc atgcgccagg
     3481 cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgtac ctcggtctcc acatttatgg
     3541 agttcatcgg taataatcct aacgccttcc ggttattatt gcgggaacgc tccggcacct
     3601 ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca cttcattgcg gaacttgcgg
     3661 actatctgga actcgaaaac catatgccgc gtgcgtttac tgaagcgcaa gccgaagcaa
     3721 tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga cgtcggcgtc gaacaacgtc
     3781 ggcaattaga agagcgactg gtactgcaac tgcgaatgat ttcgaaaggg gcttattact
     3841 ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg aaatgtgaag gacgagtaat
     3901 gaaacaagca aatcaagata gaggtacgct gctgctggcg ttggttgctg gtttatcgat
     3961 taatggtact ttcgcagcgc tgtttagctc cattgtgcca ttttctgtat tcccgattat
     4021 ttccctggtg ctgacggttt actgcctgca tcaacgttat cttaatcgca ccatgtcggt
     4081 aggcttgccg ggtctggcag ctgcctgttt tattctcggc gtactgctgt acagcacggt
     4141 agttcgtgcg gaatatccgg atatcggctc taacttcttc ccggcagtac tctccgtcat
     4201 tatggtgttc tggattggcg cgaagatgcg taaccgtaag caggaagttg ctgagtaatc
     4261 ggtatcatgc cggatgctgg cgcatccggc atgggtttta cttcgcggtc agtaatacgc
     4321 cgcactccat atggtgcgtg taggggaact gatcaaacag agccagacgt tcgaccttgt
     4381 gcgtctggct taatgtttcc agatttttgc ataacgtttc cggattgcag gagatataca
     4441 gaatacgtgg atacgcctgc accattttct cggtttcact gtccagaccg ctgcgcggtg
     4501 ggtcgacaaa aatggtttcg aactgatagc tctttaagtc gatcccttgc aggcggttaa
     4561 actcgcgcac accattcatc gcctgagtaa attcttctgc cgccatacga ataatttgta
     4621 cgttatcaat atggttagcg gcaatgttgt attgcgcggc agcgactgac ggtttggcga
     4681 tttcggtggc taatacccga tcaaaattgc gcgccagcgc taatgaaaag ttaccgttgc
     4741 cgcagtacag ctccagtaaa tcgccttttg agcctttggt tacgtccagc gcccattcca
     4801 gcatctgaat attcatcgcc gcgttcggct gggtaaagct gttttctacc tgacgataga
     4861 tcatctcttt ccctgcgacc ggcagacgct cgtcgatgta atcttgatcc agcgcgattt
     4921 tggttttcgt tgcccgacca atcagatgca cattcagatt ctgcgcgcgc agtgcatcgc
     4981 gcagtgcttc cgcttcctga cgccactcat catccagctt tttatggtac agcagggaaa
     5041 ccaccgcctg attactcagc gtggtgaggt aatcaatctg gaacaacttg tggcgcagaa
     5101 cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa ctggttgatg agttcactgg
     5161 cggcggggaa gctatccacg cggatgcggc ttttggtttg ttgatcgaaa atgatgtgat
     5221 acaggtcatc gccatcgtgc cagatgcgga actccgcgcg catccggtaa tgactgaccg
     5281 gcgagcgaaa cacttccgga accaggtcag aaaacggtgc catcatactt tgcaaacgta
     5341 ccactttttc ggctaactgc gcttcatact gttctgttgg aaggtgttcg ggggtcatga
     5401 tgtatcctga aaaattaaag tacgcggcga ttgtagggat tgctcatcag atgtccagat
     5461 cttgatgaat tcctatttgt gagctacgtc tggacagtaa cttgttacaa cctgtagcat
     5521 ccacttgccg gtcctgtgag ttaataggga atccagtgcg aatctagagc tgacgcgcag
     5581 cggtaaggaa aggtgcgatg attgcgttat gcggacactg ccatccggtg ggaagtcatc
     5641 atctcttagt atcttagata cccctccaag cccgaagacc tgccggccaa cgtcgcatct
     5701 ggttctcatc atcgcgtaat attgatgaaa cctgcggcat ccttcttcta ttgtggatgc
     5761 tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt ccgtcacggc attttccgct
     5821 tgggcacagg ataccagccc ggatactctc gtcgttactg ctatccgttt tgaacagccg
     5881 cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc aggatatcga ccgctggcag
     5941 tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg tcgatatcac ccaaaacggc
     6001 ggttcaggtc agctctcatc tatttttatt cgcggtacaa atgccagtca tgtgctggtg
     6061 ttaattgatg gcgtccgcct gaatctggcg ggggtgagtg gttctgccga ccttagccag
     6121 ttccctattg cgcttgtcca gcgtattgaa tatatccgtg gaccgcgctc cgctgtttat
     6181 ggttccgatg caataggcgg ggtggtgaat atcatcacga cgcgcgatga acccggaacg
     6241 gaaatttcag cagggtgggg aagcaatagt tatcagaact atgatgtctc tacgcagcaa
     6301 caactggggg ataaaacacg ggtaacgctg ttgggcgatt atgcccatac tcatggttat
     6361 gatgttgttg cctatggtaa taccggaacg caagcgcaga cagataacga tggtttttta
     6421 agtaaaacgc tttatggggc gctggagcat aactttactg atgcctggag cggctttgtt
     6481 cgcggctatg gctatgataa ccgtaccaat tatgacgcgt attattctcc cggttcaccg
     6541 ttgctcgata cccgtaaact ctatagccaa agttgggacg ccgggctgcg ctataacggc
     6601 gaactgatta aatcacaact cattaccagc tatagccata gcaaagatta caactacgat
     6661 ccccattatg gtcgttatga ttcgtcggcg acgctcgatg agatgaagca atacaccgtc
     6721 cagtgggcaa acaatgtcat cgttggtcac ggtagtattg gtgcgggtgt cgactggcag
     6781 aaacagacta cgacgccggg tacaggttat gttgaggatg gatatgatca acgtaatacc
     6841 ggcatctatc tgaccgggct gcaacaagtc ggcgatttta cctttgaagg cgcagcacgc
     6901 agcgacgata actcacagtt tggtcgtcat ggaacctggc aaaccagcgc cggttgggaa
     6961 ttcatcgaag gttatcgctt cattgcttcc tacgggacat cttataaggc actaaatctg
     7021 gggcaactgt atggcttcta cggaaatccg aatctggacc cggagaaaag caaacagtgg
     7081 gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc gtatttccgg atatcgtaac
     7141 gatgtcagtg acttgatcga ttatgatgat cacaccctga aatattacaa cgaagggaaa
     7201 gcgcggatta agggtgtcga ggcgacagcc aattttgata ccggaccact gacgcatact
     7261 gtgagttatg attatgtcga tgcgcgcaat gcgattaccg acacgccgtt gttacgccgt
     7321 gctaaacagc aggtgaaata ccagctcgac tggcagttgt atgacttcga ctggggtatt
     7381 acttatcagt atttaggcac tcgctatgat aaggattact catcttatcc ttatcaaacc
     7441 gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg cgtatccggt cacctctcac
     7501 ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac agtctatggc
     7561 taccaaactg caggacggga atacaccttg tctggcagct acaccttctg aaccacgtcc
     7621 caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg agatccggca
     7681 tctcttaccg gatctccatt acatttatgc tttcgataac gtcgctttcc cgtatggcga
     7741 aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt gtcaccgcgg tgcaagaacg
     7801 ttatcccctt gcgctggctg tggtcgcttg caacaccgcc agtaccgttt cacttcctgc
     7861 attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta aacctgctgc
     7921 acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaacag ttaaacgttc
     7981 ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa tgctgggctc
     8041 ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc gaagatgttt ctctggatgc
     8101 actaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata ccgttgtatt
     8161 gggttgcacc catttccctc tactacaaga agaactgtta caagtgctgc cagagggaac
     8221 ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgt tagaacatga
     8281 agccccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa tgacgccaga
     8341 agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg aaaaactggc
     8401 agttttaggc tgatttggtt gaatgttgcg cggtcggaaa attattttaa atttcctctt
     8461 gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg
     8521 ccgacgggtc agcggggttc tcctgagaat tccggcagag aaagcaaaaa taaatgcttg
     8581 actctgtagc gggaaagcgt attatgtaca ccccgcgcag ctgagaaaaa gcgaagcggc
     8641 actgctcttt aacaatttat cagacaat
//
LOCUS       DAJTRN010000144         8592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRN010000144 DAJTRN010000000
VERSION     DAJTRN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1036)
                     /gene="trkA"
                     /locus_tag="OO328_003625"
     CDS             complement(<1..1036)
                     /gene="trkA"
                     /locus_tag="OO328_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5932044.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVID"
     gene            complement(1058..2347)
                     /gene="rsmB"
                     /locus_tag="OO328_003626"
     CDS             complement(1058..2347)
                     /gene="rsmB"
                     /locus_tag="OO328_003626"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5932045.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2393..3340)
                     /gene="fmt"
                     /locus_tag="OO328_003627"
     CDS             complement(2393..3340)
                     /gene="fmt"
                     /locus_tag="OO328_003627"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5932046.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3355..3864)
                     /gene="def"
                     /locus_tag="OO328_003628"
     CDS             complement(3355..3864)
                     /gene="def"
                     /locus_tag="OO328_003628"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5932047.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4029..5153
                     /gene="dprA"
                     /locus_tag="OO328_003629"
     CDS             4029..5153
                     /gene="dprA"
                     /locus_tag="OO328_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5932048.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5125..5598
                     /locus_tag="OO328_003630"
     CDS             5125..5598
                     /locus_tag="OO328_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5932049.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5627..6169
                     /locus_tag="OO328_003631"
     CDS             5627..6169
                     /locus_tag="OO328_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5932050.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6174..6746
                     /gene="tsaC"
                     /locus_tag="OO328_003632"
     CDS             6174..6746
                     /gene="tsaC"
                     /locus_tag="OO328_003632"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5932051.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            6751..7569
                     /gene="aroE"
                     /locus_tag="OO328_003633"
     CDS             6751..7569
                     /gene="aroE"
                     /locus_tag="OO328_003633"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5932052.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7566..7823
                     /locus_tag="OO328_003634"
     CDS             7566..7823
                     /locus_tag="OO328_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5932053.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(7799..8353)
                     /locus_tag="OO328_003635"
     CDS             complement(7799..8353)
                     /locus_tag="OO328_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5932054.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2135 a         2095 c         2157 g         2205 t
ORIGIN      
        1 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
       61 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
      121 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
      181 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
      241 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
      301 ctgcaccgat attaccgcca ccaaccagca tgatccgttt atacggtttt tccagtcgct
      361 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
      421 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
      481 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
      541 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
      601 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
      661 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
      721 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
      781 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
      841 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
      901 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
      961 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
     1021 gaatgataat tttcatcagt cgcgacccgt tatctcatca ctttttgatt agcttagcgt
     1081 aaaagaagcc gtcgccctct tcggcaccag gctgattttg tttacccggt tgctctggtg
     1141 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc
     1201 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
     1261 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
     1321 cgcgacgtaa ccatttaata tctggatggc gacgaatcac ccccgttgct gaacaaggcg
     1381 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
     1441 caccttgttt cacggttgcc ttcataccaa ggcgttttaa attgtcgtaa atgcgagaga
     1501 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
     1561 gcgttgtttt accgccgggg gcggcacaaa gatccaagat gtgttcaccg ttttgtggcg
     1621 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
     1681 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
     1741 catgcgggaa acctttcatt cctgcttcat ccagcaatgc aagccagctg tcgcgggaat
     1801 gatgcgtacg attaatacgc agccacattg gcggacgctg gttattggct tcggcgatgg
     1861 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
     1921 aacgtgcatc acgggtatta aactcggcta ataactcttc ttgccgacgc tggaactggc
     1981 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
     2041 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
     2101 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
     2161 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
     2221 gtgctttgtc tttatcggaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
     2281 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
     2341 ttttcataag tataaaaatg ctcggcaaga ccgggcttag aagagtggac tatcagacca
     2401 gacggttgcc cggaacaaac cattcccgac gagagttcag gaggtcttgc gcgctcatcg
     2461 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
     2521 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
     2581 tgaccgacgc tttccagact ttaaccggct gtccttcaat ttccagccag ctcattggcc
     2641 atgggttgaa agcgcgaatg cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac
     2701 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
     2761 gtttcgccgt gccgtctgcc aattgtttca acgtggtgat aagcccttgt gggccaagct
     2821 ctgccagctt gtcgtacaac gtaccactgg tatcttctgc agtaatcggg caggagagct
     2881 tatagagcat gtcaccggtg tctaaaccaa catccatttg cataatggtt acaccagttt
     2941 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
     3001 gtgaaccatg aatgttgata cagccaagac gcggcatctc cagcacagct ttaggcagaa
     3061 ttaaaccata ggcgacgacg accataacat cagcctgcaa atcggcgacc agttgctggt
     3121 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag accttcttcc tcagccagaa
     3181 ctttaatcgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
     3241 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
     3301 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
     3361 ccgggctttc agacgatcca gtttttcaac tttctgacga attcgttgtt gtttcagcgg
     3421 tgacagataa tccataaaca gtttgccaac caggtgatcc atctcatgct gaatacagat
     3481 agctaacaga ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
     3541 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
     3601 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
     3661 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
     3721 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
     3781 aatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
     3841 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggagt tagaaagatt
     3901 atttcttcta ttctagacaa atccccctct gattgacagc atcacttgat tgacagcatc
     3961 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4021 agataaggat ggtcgatata gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4081 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4141 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4201 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4261 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4321 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4381 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4441 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4501 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4561 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     4621 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     4681 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     4741 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     4801 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     4861 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     4921 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     4981 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5041 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5101 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5161 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5221 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5281 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5341 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5401 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5461 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5521 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     5581 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     5641 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     5701 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     5761 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     5821 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     5881 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     5941 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6001 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6061 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6121 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6181 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6241 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6301 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6361 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6421 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6481 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6541 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     6601 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     6661 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     6721 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     6781 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     6841 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     6901 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     6961 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7021 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7081 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7141 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7201 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7261 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7321 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7381 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7441 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7501 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7561 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     7621 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     7681 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     7741 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     7801 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     7861 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     7921 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     7981 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8041 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8101 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8161 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8221 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8281 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8341 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8401 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8461 aaagggtgaa aaaacaacaa acagaaaaga agatcaaaaa aatacttgtg caaaatattg
     8521 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     8581 aacaacgaag ag
//
LOCUS       DAJTRN010000145         8373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRN010000145 DAJTRN010000000
VERSION     DAJTRN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="OO328_003636"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="OO328_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="OO328_003637"
     CDS             1128..1235
                     /locus_tag="OO328_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932055.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="OO328_003638"
     CDS             complement(1288..2046)
                     /locus_tag="OO328_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5932056.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="OO328_003639"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="OO328_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5932057.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="OO328_003640"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="OO328_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5932058.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="OO328_003641"
     CDS             3759..4619
                     /locus_tag="OO328_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5932059.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="OO328_003642"
     CDS             complement(4660..5196)
                     /locus_tag="OO328_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5932060.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="OO328_003643"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="OO328_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5932061.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="OO328_003644"
     CDS             6174..6764
                     /locus_tag="OO328_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5932062.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="OO328_003645"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="OO328_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2170 a         2004 c         2009 g         2190 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctgggcc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact cat
//
LOCUS       DAJTRN010000146         8331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRN010000146 DAJTRN010000000
VERSION     DAJTRN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..157
                     /locus_tag="OO328_003646"
     CDS             <1..157
                     /locus_tag="OO328_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932063.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO328_003647"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO328_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5932064.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO328_003648"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO328_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5932065.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="OO328_003649"
     CDS             873..1244
                     /locus_tag="OO328_003649"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5932066.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="OO328_003650"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="OO328_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6146..6892
                     /gene="traX"
                     /locus_tag="OO328_003651"
     CDS             6146..6892
                     /gene="traX"
                     /locus_tag="OO328_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5932067.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6947..7507
                     /locus_tag="OO328_003652"
     CDS             6947..7507
                     /locus_tag="OO328_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5932068.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7638..7850
                     /locus_tag="OO328_003653"
     CDS             7638..7850
                     /locus_tag="OO328_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5932069.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8094..>8331
                     /locus_tag="OO328_003654"
     CDS             8094..>8331
                     /locus_tag="OO328_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5932070.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2086 c         2671 g         1662 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cttgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcca gtgcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc actgcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa ctccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     6181 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     6241 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     6301 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     6361 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     6421 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     6481 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     6541 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     6601 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     6661 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     6721 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     6781 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     6841 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     6901 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga cagagcagaa
     6961 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     7021 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     7081 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     7141 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     7201 gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     7261 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     7321 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     7381 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     7441 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     7501 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     7561 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     7621 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     7681 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     7741 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     7801 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     7861 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     7921 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     7981 tcgatttttt atattttgtg attagtttca caaaataagg tgttattcaa tgtgtgctgc
     8041 actattcagg ggggctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     8101 aatatattcc actggcatta tttatctttt catggccggt attgtctgca gatattcatg
     8161 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     8221 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     8281 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca t
//
LOCUS       DAJTRN010000147         8308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRN010000147 DAJTRN010000000
VERSION     DAJTRN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..188
                     /gene="csgC"
                     /locus_tag="OO328_003655"
     CDS             <1..188
                     /gene="csgC"
                     /locus_tag="OO328_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5932071.1"
                     /translation="SSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSD
                     GQALHLSQQWTPSSEKS"
     gene            309..620
                     /locus_tag="OO328_003656"
     CDS             309..620
                     /locus_tag="OO328_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5932072.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            715..1248
                     /gene="ymdB"
                     /locus_tag="OO328_003657"
     CDS             715..1248
                     /gene="ymdB"
                     /locus_tag="OO328_003657"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5932073.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1190..2671
                     /gene="clsC"
                     /locus_tag="OO328_003658"
     CDS             1190..2671
                     /gene="clsC"
                     /locus_tag="OO328_003658"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5932074.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO328_003659"
     CDS             complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO328_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5932075.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4230..5765
                     /gene="mdoG"
                     /locus_tag="OO328_003660"
     CDS             4230..5765
                     /gene="mdoG"
                     /locus_tag="OO328_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5932076.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPLPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5758..8301
                     /gene="mdoH"
                     /locus_tag="OO328_003661"
     CDS             5758..8301
                     /gene="mdoH"
                     /locus_tag="OO328_003661"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5932077.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2100 a         1967 c         2171 g         2070 t
ORIGIN      
        1 gcagttcagg gcaaagtcag acgaagcaag aaaagaccct ctcattgcct gctaatcaac
       61 ccattgcttt gacgaagttg agtttaaata tttccccgga agatcgggtg aaaatagttg
      121 ttactgtttc tgatggacag gcacttcatt tatcacaaca atggacgccc tcttcagaaa
      181 agtcttaatt tgttgaaata tcgagcataa gatgaatctg gagagaatgg tctgctgcga
      241 atcagccaac ctgaaagtat ggataacaca accttcaagg atgactaatc attgaggaaa
      301 tagaataaat gttcagacct tttttagact ctcttatgct cggcagtttg ttttttcctt
      361 ttattgccat tgcaggaagc accgcgcaag ggggcgtgat ccatttttat ggccaaattg
      421 tggaaccggc atgtgacgtc agcacccagt catcacccgt agaaatgaac tgcccacaga
      481 atggttctat tccgggcaaa acctactcca gcaaagcgtt aatgagcggc aatgtcaaaa
      541 acgcgcaaat agcctcagta aaagtgcagt atcttgataa acagaaaaag ctggctgtaa
      601 tgaacatcga atataactaa gtttctgaac aactcacgtt gctgagcaga aaaatgcgat
      661 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
      721 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      781 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      841 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      901 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      961 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
     1021 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
     1081 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
     1141 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
     1201 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
     1261 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
     1321 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
     1381 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
     1441 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
     1501 gcctggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
     1561 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
     1621 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1681 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1741 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1801 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1861 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1921 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1981 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     2041 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     2101 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     2161 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     2221 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     2281 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     2341 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     2401 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     2461 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     2521 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     2581 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     2641 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2701 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2761 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2821 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2881 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2941 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     3001 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     3061 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     3121 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     3181 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     3241 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     3301 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     3361 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     3421 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     3481 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     3541 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     3601 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3661 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3721 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3781 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3841 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3901 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3961 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     4021 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     4081 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     4141 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     4201 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     4261 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     4321 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     4381 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     4441 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     4501 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     4561 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     4621 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4681 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4741 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4801 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4861 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4921 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4981 ccagtatgtt cctgtttggg ccgaaccaac cgttgcctgc aaataactat cgtccggagt
     5041 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     5101 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     5161 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     5221 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     5281 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     5341 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     5401 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     5461 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     5521 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     5581 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     5641 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5701 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5761 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5821 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5881 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5941 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     6001 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     6061 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     6121 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     6181 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     6241 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     6301 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     6361 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     6421 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     6481 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     6541 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     6601 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6661 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6721 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6781 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6841 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6901 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6961 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     7021 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     7081 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     7141 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     7201 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     7261 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     7321 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     7381 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     7441 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     7501 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     7561 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     7621 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7681 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7741 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7801 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7861 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7921 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7981 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     8041 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     8101 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     8161 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     8221 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     8281 ccggatgcgg cttcgcaata aaaacgta
//
LOCUS       DAJTRN010000148         8224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRN010000148 DAJTRN010000000
VERSION     DAJTRN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..305
                     /locus_tag="OO328_003662"
     CDS             <1..305
                     /locus_tag="OO328_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5932078.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2187
                     /locus_tag="OO328_003663"
     CDS             277..2187
                     /locus_tag="OO328_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5932079.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2188..2391
                     /locus_tag="OO328_003664"
     CDS             2188..2391
                     /locus_tag="OO328_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5932080.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="OO328_003665"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="OO328_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="OO328_003666"
     CDS             3971..5476
                     /locus_tag="OO328_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5932081.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="OO328_003667"
     CDS             5513..5860
                     /locus_tag="OO328_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5932082.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="OO328_003668"
     CDS             5918..6946
                     /locus_tag="OO328_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5932083.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="OO328_003669"
     CDS             6998..7384
                     /locus_tag="OO328_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5932084.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="OO328_003670"
     CDS             7396..7773
                     /locus_tag="OO328_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5932085.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..>8224
                     /locus_tag="OO328_003671"
     CDS             7760..>8224
                     /locus_tag="OO328_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5932086.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1906 a         1932 c         2540 g         1846 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacac
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgcgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccg
//
LOCUS       DAJTRN010000149         8152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRN010000149 DAJTRN010000000
VERSION     DAJTRN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..540
                     /locus_tag="OO328_003672"
     CDS             <1..540
                     /locus_tag="OO328_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932087.1"
                     /translation="DYFDMLQEELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPF
                     GDIKSDGTVKTALENLGLGEAAKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLIL
                     QCGQLNGAPNVSSTYGMRFPMTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITV
                     SPGSGYGSSADAYYIAMGY"
     gene            512..946
                     /locus_tag="OO328_003673"
     CDS             512..946
                     /locus_tag="OO328_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5932088.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1719..2768)
                     /locus_tag="OO328_003674"
     CDS             complement(1719..2768)
                     /locus_tag="OO328_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5932089.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3219..4409
                     /locus_tag="OO328_003675"
     CDS             3219..4409
                     /locus_tag="OO328_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5932090.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4799..6259)
                     /gene="gtrII"
                     /locus_tag="OO328_003676"
     CDS             complement(4799..6259)
                     /gene="gtrII"
                     /locus_tag="OO328_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5932091.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6256..7185)
                     /locus_tag="OO328_003677"
     CDS             complement(6256..7185)
                     /locus_tag="OO328_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5932092.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7182..7544)
                     /locus_tag="OO328_003678"
     CDS             complement(7182..7544)
                     /locus_tag="OO328_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5932093.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7806..>8152)
                     /locus_tag="OO328_003679"
     CDS             complement(7806..>8152)
                     /locus_tag="OO328_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5932094.1"
                     /translation="TKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLR
                     HTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNT
                     AQEENVVYLNAR"
BASE COUNT         2493 a         1563 c         1652 g         2444 t
ORIGIN      
        1 gactactttg acatgttgca ggaggagctt tgcagcgtgg tggaggcatc cggtgccagc
       61 ctggagaagg ggcggcacga tcagttgctt accgcacttc gcgcgctgct gttaagccgc
      121 aagaatccgt ttggcgatat caaatcggat ggcacggtga aaacggctct cgaaaacctt
      181 ggtttgggag aagcagctaa aaggaatgta ggtacagggg cgaatcagat acctgatatg
      241 agcctgttcg cgtcaattaa taccgtaacg gctgctgcgc aaaaatttcc gtctggatta
      301 attttacagt gcggtcagtt gaatggtgcc ccgaatgtat cttcaacata cgggatgagg
      361 ttcccgatga cgttctcaag agtaattgct gtcgtagtta cattgaacgt tactggcgcg
      421 gctgggcagc cgactgtatc ggcgacaagt gtccagaaca ctggatttaa tattactgtg
      481 tcgcccggtt caggatacgg ttcatctgct gatgcgtatt acattgcaat gggatattaa
      541 caaaatgtca tatttttatt ctgcatcgac aaacggattt tattcgactg aatttcacgg
      601 caccaatatt cctgatgatg cagtggaaat ctcggaatca gagtggaaaa cactgattaa
      661 tgcacagagt gtaacaaaaa tgattacctg tggtgagaac ggtcatcctg tcattgttga
      721 ccgtccttct ccaacaccag aacaattagc cttaataaat gatgaaaaga aatctgcact
      781 gatagcagag gcaacgaatg taatagctcc gcttcaggat gcggttgatt taggtatggc
      841 aacagatgat gaaacgaaac tgttactggc atggaaaaaa tatcgggtgc tgcttatgcg
      901 tgttaatgta gtaaaacccg agtggcctat gcatccaaat aaatagactt gttaatctag
      961 gataattgtt ggaataatac tcgtttttca gtaggttcag gtagcctggg tataatgctc
     1021 acttctacaa tgatttcaac ttattttatt taattgatat tacgaaactt aactcataca
     1081 taaacaatat gactcaatac tgtcctaaat agctgcgcag aaaagtgctc cactttcgct
     1141 cagcccttgc tgggcgaagc atcaggaagg cgttctcagc taggttgtgt tatatctggc
     1201 ttgagttttg ttgcccatac gcaacacatt acccatctgt aaacgacaag tgtggcagaa
     1261 ttaggtgcag ctatttagac tatatcgacg cagtaacgat aatagatatc gaaactgtac
     1321 tagatattag ctacttatgg ttaccggggg gagtcttttg agcactggtg ttatcaattg
     1381 gcttcaaata attcgccaat aaattatatt gcgatagctc atcaccttcc aggttgttta
     1441 tagccagttt atcagcgtca ccacctactg tttatgcttt tatttctttg attaataagt
     1501 atatgatatt ttaattcatc tggtgtattt ctttctcatg tgttggtcga ctgaatatct
     1561 gttcagtgaa ggggtaattt tatgtttgca cttcgaacat tcccttcagt tgctaggaaa
     1621 gaaaaccgta acatttgcgc attaatttta acagtataat ctgagcataa atatgcgcta
     1681 tatatctata aaaaggcgca attaattgcg cctttttatc attttatcaa aaatggccca
     1741 ccgactgtcc tcgatagcca atggccaatt ttatctgata catatacaca aatacaggta
     1801 tatatgattg cgcagatagg atttggaata gtcacgctga agcctataat tgctaaagcc
     1861 atagggataa ataatttggt gatatactcc attccacata tgttaagagt atttcttcca
     1921 gctcttacaa taaagtcatt tccacaaatt atttttgcta tgaaaatatt aaagattatc
     1981 aagcctaccg tggtgataat agtattaata gcttgaatat aactacttga tattatgtct
     2041 gcattgaata ttgagataat cttaaaaagt aaaggtttct ggttaagtag ttgatacgca
     2101 ctcattatag cggtaattga gaagacaatg aaaccaattt tagattttcc aaaaaacctg
     2161 tcacgtgtta attctaaaaa catacatcta ccgagaggca ataaccacca gtatgccata
     2221 gcggagtcta tgttccaaaa ccattgagga tctagtaatg ggttatgatt gagcactgtt
     2281 tgtgacaaca tgaacgataa cagtgatatg attaaaatca ctatattgtt cttgactatt
     2341 tctctaaaga ttgcatcaat tgctattata acaaaaaggc agtttataaa ccatattgtt
     2401 cctacgaatt ggttatttct aacaccatat attatatcat aaatgtggtt gtatatataa
     2461 tcaatcgtct cacctgagtt aattgtgttt attattaata tagaaaatgc aaaagtgaaa
     2521 tatggaatca ttaatctgta aaactttgac ttgatataat cgaaaacaga taagtcattt
     2581 tttttgatag taaaaaaacc agcagcgaaa aagaatagtg ggacgtgata actaaaaaca
     2641 aaaggataca atttgccagc ccccaaccca aggtgaccta aatatatagc aaaaataccg
     2701 agaaatttta aagcatcaac ccaatcatgt cttatttttc tgttgctgtc gattttgtta
     2761 ttatacattt aaagtaggct ctccagtttt gtgtgatttt taaacactca ttgcaaatat
     2821 gaatgcctga aagtttatga ttattaatca acgagttcaa tattaattta ttatttttca
     2881 aagtcgcact atataggcat ttacttccgc agtaccaccg atagtatcga tatcaaatca
     2941 gcctgcggcc gcgataactt tcgtaacatt ctgccagcgg ctttgttgaa taaatcaggt
     3001 tcaagataat cgtctctgca tcaggttaga gtttcaggca atacatcctc tttctggcat
     3061 acctgccact ctcgcaccat ggtcatagcc tttacgatct gcatactcac cagaccagag
     3121 cccgctccct tttaggctga ggaatcccca gaaaagaaga caggcataga gttttgtgat
     3181 ctactgattg cgctaacaaa ttcaatgaga tcacctctat gcctgcccgt aaagtatgcc
     3241 agactttttt ccgtaatgct ttagcttcca cgcatcagta tcgacaaaat gccattattg
     3301 attctgctgc tgctttagtc ggtggcgcat ctctttctct taccagcatt ggccggcatt
     3361 tacccggtcc tgctcgcgtg aaagataaaa taaaacgcgt tgactgtctg ttgggtaata
     3421 aacgacttca taatgatatc cctctgatat tcaaaaatat tacatccatg atgacaaata
     3481 aactgtcgtg gtgtgttatc gctgttgact ggagcggtta cccttttcag gaatatcatg
     3541 tccttcgtgc cagcctcctc tgcgacggtc gctctatccc cctgatgagt caggtcttcc
     3601 cgtcaaaaaa aaataacgag gcggtggaaa tcgcttttct ggatgcgctt tccggggcca
     3661 tttctccccg gactcgtgtt gttattgtta ctgatgctgg ttttcaaagt gcatggttcc
     3721 gtcatatcaa atcacaggga tgggatttta ttggtcgtat caggggggta gtgaagttca
     3781 ggctggatag tgacaaagat aagtggctgg acataaaaat gtgcaggggg agttcagagg
     3841 caaaatacct ggggacggga accctggccc ggaaaaaacg ctcgcagtgt gaaggacatt
     3901 tttatcttta taaacattca cctaaaggca gaaaaagcag gcgtgccaga gggaggccgg
     3961 gcttaccaac aacggaaaaa gaacaaaaag ccgcaggcag agaaccatgg ttaatattca
     4021 gcaatacagc tgagtttaat gccaaaaaaa tcatgaaact gtacagtcga agaatgcaga
     4081 tagagcaaaa ctttcgggat gaaaaaagtg aacgctttgg ttttggcctt cgggcgagca
     4141 gaagtcggag gggagagcgt tttctggtat taagtctact ggttacactg gccagtatcg
     4201 tactctggct acttggttat cattttgaaa ataaaggatt tcatttaaag tatcaggcta
     4261 atagtctcaa aaaacgaaga gtattgtctt ttctgacgct ggctgaaaac gtcctgagat
     4321 ttaaccctga acttctacga cgagcacaac ctgaaaagat attgtgccgg ttggccagca
     4381 cttaccgaag tatggtgctg gcttactgat gaaaattttc tggggatccc tcagcctttc
     4441 ggggaggaat gagggctctg atattctttt gccgtagttc atcgtgaaac cgtcggatgt
     4501 catttggcct gagtgaccca tgcctcccaa aagcagagtc gctagtgtta tgtaattagg
     4561 cggttaaact tcaattctca gtgttaagtt tgggtaaacg cttgtaaatg gtattgctaa
     4621 ttttttaatt gactaatatt agaaacggtt agtgtgattg tatatagata tttgagtgta
     4681 aggtatttaa aggggcattc agcccctccg tctatttgtg attgcatcca ttgatgtgtg
     4741 tattattttg agcttgaaaa agggaggcgc tctttaataa taaataaatc tatcaaagct
     4801 aaatattaaa tggaagccac ccttttttct ctgatagttt tattatcacc aaaccattta
     4861 tttcagctat cgaatctgga ttcggccagg aaggtaaact cttcacaatt ggataaatct
     4921 tctctatttg ttctttattt gctggtttgc aattacagat tccatagtaa ttcataaatg
     4981 caaccatcct aaaatagttg ccaccatccc accaaaaaaa agattttcca aaagtgtctg
     5041 cgctcatgga gagcatattt cctgagtcgt ttgaaccata tatatatata tattttccgt
     5101 taagggaaat gccttttgtt tgagatgtgt atgatatttc tttagctaaa gagatatctt
     5161 tctgtcttgc gagagtatca ttcaaaaata gatttttgga tgttgaaacg ccattaaata
     5221 taataaataa aaaaaacatg cagtttaggt attttataga gcgaaaattg cttaagcaag
     5281 aaaacaaaat tactgctact ataggcatta aaacaaacag gcgaggtggc gcccctgagc
     5341 caacaacaac gataaatata accggaagta tataaatgaa aatgattgat gaaatcaaat
     5401 aaattgatct tgtttttaat ttatatgtta gggtaaacat cagaattaat aaaggaatca
     5461 gtaaccactt gtataggttt aaaccagtgg gaggattgtt atagaagtta tatatattat
     5521 ctatggctga tttaacaagc catttaatat cgcttgcatt ttgtatataa tttgagatgt
     5581 actcgttcga ttcaagcgaa aaataatgct tgatagcttt ggttaatagc aggtaaatta
     5641 aggtagataa agctatgagg gatagagata aacatgaact ataaaaagat tcacgaatat
     5701 ttttctcatt tcgtattatc gaatttatct gcaacccaat gacgaatgca ataatatatg
     5761 ttacgaatgt ttggtagatt gccattgaaa gaatggacag tactatacca gaaaatatca
     5821 caatcctatt tttttgcgag tgaaaaataa ttgctgatat cgctgccagt agaaaagcaa
     5881 ttcccacagt atcagcttgg ttaagaaact ctaattgata ggagatctga gggaaggtaa
     5941 taaatactaa catacctatc aataattcat aagattctag ctttagcgat ctgcagatta
     6001 taaatgctga aatgatgata aaagataagg ctattaatgg tgttatataa agtgaaaaag
     6061 gctcagggag aaagtaatgt cgcaaaaaag tgtgaaacca ccgcccaagc gtgattgttt
     6121 gataaaaatt atttgtaaac tcgccatcaa tattaagtgt tccatcgtac aaccaccccc
     6181 aataacaata aattgatatt gcaaaacatg atatgaaggc aaggagattt gcatttttaa
     6241 ataaattaat tttaatcatg ggttacctcg atgagatttt tttatgatgt atttgggtct
     6301 attttttact tcaatataga ttctacctat atattctccg agaacaccaa tcccgatcag
     6361 ttgcactcca cccaagaaaa gtattgatac aagcagggag ggatacccgc gtactgggtt
     6421 accaaagaca agggtgtcta taatcatcca tgcaccatat aaaaatgaaa tgcttgcaac
     6481 aaacaagcct atataagtcc atacgcgaag aggaaaggtt gaaaaacttg tgataccttc
     6541 cagtgccaga ttccataatt tccagccatt aaattttgag atgccagcaa cacgctctgc
     6601 gcgtacatat tcaacgacat ccgtcggacc acccacccag ctcagtatgc ctttcatgaa
     6661 aagattgcgc tcaggcaaca gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa
     6721 atctccgaca ttttcctcga tctttggggt gcttattttg ttgtgtaatt tatagaacca
     6781 ctcagctgtc ttacgtttca gtcgtccatc agttgagcgg tctgaacgtt tagcaagcac
     6841 catgtcagca cctgcctgcc acttttcaat aagatgaggg ataacctcaa ttgggtcttg
     6901 caggtcgaca tcaataggaa ttacagcatc gccgcttgca tggtctaacc ctgcaaataa
     6961 ggctggttct ttaccaaagt tgcgtgtaaa tgacagcgga acaactagcg ggtctgaaac
     7021 agccagcgcg ttaataattg actctgtggc atctttactt ccgtcattta tgaatacaat
     7081 ttctacttca tatggcttca actcttggaa ttcacgtacc gttttataga aaacaggtat
     7141 cgcttcttct tcattgaaga caggaacgac aagagatatt ttcatttcgc atccctaaag
     7201 acaatgaact ttgaatagac gaaaccgcac accagactga tggcggagaa ggtgataaga
     7261 gtgactattg gaggaagtga gcatttatca gctgcccaac caacaatcac actcaatatt
     7321 cccataaatc ccacgtataa catgtagcgc atcgctgtag tcaatgcttt gaatgtgaat
     7381 cttgcattcg cgaagaagct aaagctcaca gccactacga aacctgtgaa gtttgccaga
     7441 gcctgactgg tatgtgcggc atagatacat acaccaaaaa ccacccagtg tataagtgtg
     7501 ttcagcacac ctatagatgt gtactttaca aataacttta acatttattt aatcaatgag
     7561 ctctgaaagg catgaagtct atcatccaag tctcaattga tcgatacttg ctatgtctga
     7621 tgagacaaaa ctgagacaca taaggcctca caatggcttg caaggcttta catgttttga
     7681 tgtggtggga cgtgtgagcg cagtgttgat gggataatcc tttgaattac aagcggattc
     7741 ttataattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc atttaaatca
     7801 ataagttacc tcgcatttaa gtaaactacg ttctcctctt gtgccgtatt tgtgccattg
     7861 cgacttataa tcgcatcgat tttgctcgcg tgctcggtaa gatgcccggc tgaaaggtgg
     7921 gcgtatcttt gaaccatttc gagagtttcc catcctccca tctctttaag cgcaagaaga
     7981 gagacaccgg actgaaccag ccagcttgcc caggtatgcc tcaggtcatg gaagcggaag
     8041 ttgctaatgc ctgcccgctt taacgctccc ttccatgcct tgttgctgtc ggttctcatc
     8101 ttccttaccg ctgctgtttt tgttccgtcg cttcggtagg caggtttggt gt
//
LOCUS       DAJTRN010000150         8050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRN010000150 DAJTRN010000000
VERSION     DAJTRN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..2048)
                     /gene="uvrD"
                     /locus_tag="OO328_003680"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(<1..2048)
                     /gene="uvrD"
                     /locus_tag="OO328_003680"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5932095.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     "
     gene            complement(2132..2848)
                     /gene="yigB"
                     /locus_tag="OO328_003681"
     CDS             complement(2132..2848)
                     /gene="yigB"
                     /locus_tag="OO328_003681"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5932096.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(2848..3744)
                     /gene="xerC"
                     /locus_tag="OO328_003682"
     CDS             complement(2848..3744)
                     /gene="xerC"
                     /locus_tag="OO328_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5932097.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(3741..4448)
                     /locus_tag="OO328_003683"
     CDS             complement(3741..4448)
                     /locus_tag="OO328_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5932098.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4445..5269)
                     /gene="dapF"
                     /locus_tag="OO328_003684"
     CDS             complement(4445..5269)
                     /gene="dapF"
                     /locus_tag="OO328_003684"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5932099.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5306..5509)
                     /locus_tag="OO328_003685"
     CDS             complement(5306..5509)
                     /locus_tag="OO328_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5932100.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            5698..7176
                     /locus_tag="OO328_003686"
                     /pseudo
     CDS             5698..7176
                     /locus_tag="OO328_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7173..>8050
                     /locus_tag="OO328_003687"
     CDS             7173..>8050
                     /locus_tag="OO328_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5932101.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPG"
BASE COUNT         1860 a         2229 c         2072 g         1889 t
ORIGIN      
        1 tcaccaaact tagcgtggcg tacacgctgg ccgagcttgt agccgctgtc gttctcgacc
       61 atcggcgtac ccatccgctg atggctgacc gggcggctta ccgtggcgcg caggcgcacc
      121 tcttccacac actcttccgg caactcgcca ataaagcgcg acgggcgatg gtaaacctct
      181 ttgccataca gacggcgagt ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt
      241 acgccaacgt aggccagacg gcgttcttct tccagacgcc cgccttcatc cagcgacatc
      301 tggcttggga acatgccctc ttccataccg acgataaaca cctgcgggaa ctccagacct
      361 ttcgccgagt gcatggtcat cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg
      421 cctgcttcca gcgccgcatg ggataggaac gcctgcagcg gcattaaatc ttcgtcttct
      481 tcgttgtagc tgaactggcg cgttgccgtc accagttcct ctaagttttc gatacgcgtc
      541 tgaccttttt cgcccttctc ctgctcgtac atggtacgca ggccggagtc tttaattacc
      601 cggtcagtct gtacatgcag cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt
      661 tccataaacc gctgtaaggc gctggcagca cgtccggcga gggctttttc ctgcaacagt
      721 tcacgacatg cctgccagag tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc
      781 agcgtccggt caccaatacc ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg
      841 tcgttacggt tggcaatcag gcgcagatac gagagcgcat ctttgatttc ctggcgttcg
      901 aagaagcgca tcccgccgta aatacggtac ggcatactcg cctgcaacag tgcttcttcc
      961 agcacacgcg actgggcgtt actgcggtag agaatggcgc actcggcaag cgccccgccg
     1021 ttgtcctgcc aggttttgat gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa
     1081 gcgcaataga gggaaatagg ctcaccgtcc gcgccatcgg tccacagttt tttacccaga
     1141 cgcccgttat tgttttcaat cagggcgtta gcggcgctca gaatattgct ggtagagcgg
     1201 tagttttgct ccagacgaat aatttcggca ccggggaaat cattaaggaa acgctgaata
     1261 ttctccacct gcgccccgcg ccagccgtag attgactggt cgtcatcacc gacgatcatc
     1321 actttgccgg tgtcgcccgc cagcaggcgg atccacgcgt actgaatgtt gttggtatcc
     1381 tggaattcgt ccaccaggat attggtaaaa cgttcgcggt agtgttgtag gatatgcggc
     1441 ttgttaagcc acagttcgtg agcgcgcagc agcagctcgg cgaagtccac caggcccgcg
     1501 cgatcgcacg cttcctgata cgcctgatac accttctgcc aggtctgctc caccggatta
     1561 ccgtagcttt gaatatgatg cggacgcagg ccttcatctt tctggctgtt gatgtaccac
     1621 attgcctggc gcggcggcca ctgcttctcg tcgaggttca tggctttgat cagacgctta
     1681 agcaggcgta gctggtcttc gctatcgagg atctggaaat cctgcggcag attggcgtcc
     1741 atatggtgcg cacgcagcag gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg
     1801 ccctggctgg tgcccatcag ttgtccgata cgatgacgca tctccgccgc cgctttgttg
     1861 gtaaacgtca cagccataat cgagtatggc gagcagtttt ccacgctcat cagccaggcg
     1921 atacgatgca ccagtacgcg cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg
     1981 ctgcgtggcg cggccaccgc ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa
     2041 acgtccattg gcaccgccgc gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc
     2101 aaaaagctgg gtatatatac agatttgctg attatatcag cgaggtcaga gatgccaacc
     2161 gggaaatttc cagatgtggc agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt
     2221 ccggtctgat ccaacaagcc tgcattccgc tgcgaattgc cccacccacg tcagtggtga
     2281 gatcgtcccc aacatgtaag atctcgccga tcggcacgtt gagtttttcc gcagccaaaa
     2341 agtacatatc gctgaacggt tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa
     2401 aataatcccc caaaccaaac agttccggct gggcgttacc gttggtgatc gccaccagcg
     2461 gccatttctt cgccagctgt tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc
     2521 tgcgccattt ggcaaagtta atcattgctg cgtgtgcgcc tgcactggct tcttcggcac
     2581 tcagcccggc gtcgagcatc gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt
     2641 gataaatctc gggttccgct tcccgtaccg cctggcgcag gcgttgcaga tcttcattct
     2701 ggaagctgcg cagcgccgga tgataatttt gcacaaaggt aagcgcctct cgctcggtgc
     2761 gcaaaatcac cggacggtta tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga
     2821 tgcgccccaa aggccggtaa aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc
     2881 atcgtacacc gaggcaaggt gttgaaaatc aagatgagta tagatttgcg tggtggagag
     2941 gttggcatga cccagtagct cctgcacacc acgaagatcg ccgctcgact ccagcatatg
     3001 cgtagcgaac gagtgacgta atttatgtgg gtgaacgtga ttattcagcc cttgttttat
     3061 gccccattcg gcaaagcgtt tctgcacatt acgcgcggag atacgcttgc ccagtttcga
     3121 cagaaaaagc gcgtcgtctt cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat
     3181 ccacgccaca gcgttgcgac caatcggcag gcggcgctct ttgctgcctt tccccatcac
     3241 ccacacttcg ccggactcca ggtcgaggtg tttgatatcc agccccacca gctcggaaag
     3301 acgcagaccc gcgccgtaca tcacttccag cattgcacgg tcgcgtacag cgaggggatc
     3361 gttgatatca atatccagca gccgattcat atcgtcgacg tcgatatttt tcggcagatg
     3421 acgcggcgct ttcggtgccg aaactccttt cgccgggtta gctttgagtt cgttctggct
     3481 gaccagccag tcaaaaaagc tacgtagcgc agaaagccgt aacgccagac ttgctgctcc
     3541 cagcccttta cggcgactgc gtacagcaaa attgcgtacc atcgctgcat cgcattgttg
     3601 ccagctttgc aggccgtttt cgctggcaaa attgatgatc gcctcaagct gacgctggta
     3661 gttaagtagg gttatcgggc taagctgacg ctccacgctc agataacgta ggtagcgttc
     3721 tacatcggtg tgtaaatcgg tcatacgcgt tcaatccaac gctccagaag ctccggcaac
     3781 atcagcgcaa tttcatgaag taactgcgtt ccctgccctt gttgatagtg actggcatcg
     3841 cgactggtaa acagcacaac acccaaatca gcatcgcttc ccagcatcga catcgccacc
     3901 gatcccaccg ctttcgcttc cggtagcacc accagcagct ccggtccgtt aagcggccca
     3961 agatagtgct gttcctgccc caaacgctga atacgcagcg gttcgaaaga ctgacggctt
     4021 aatgccagat gagtgtggtt cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc
     4081 agactcgcac cagctaggcc gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg
     4141 tcgagactgc tggcggcggt gagactgcgc tgcaggtaga gtaggcgata aaacagccct
     4201 tcgttggcga tagcctgctc catcaacaac gccatgttct cttccagaac atgaatgtga
     4261 ttacgtgcgc gggccatgtg ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc
     4321 acacgtatcg cttctactgc gcgcgcattg cggataaaaa actcaggatt tttaatcaga
     4381 taatcgacaa ccgcccggtc atcaagctcc gtgagtgttt cctgcagttc ttcccctggt
     4441 tgcttcatag atgaataaat ccatcgtaga catgtaccgc cgggccagtc atatataacg
     4501 ggtgacccgg acctttccag gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt
     4561 cttcgtccag caaaccttgc tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc
     4621 aggcctgggt ttctcctgcc ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa
     4681 ccacttgcat aaaaccgata ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag
     4741 gaccaagcgt ttctaccgcc gcggtatcga catcatcgac ctgaatcacg caatgcggat
     4801 ttcccatcga caccacgccg cataagattg tctgctcggc ggcgcgcata atataggtct
     4861 tttccgcttt gttagcgcga aacggcacgg cggaaggttc gaagttgggt tcgcccatat
     4921 ttacgcggac cagatcatca tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga
     4981 cgcggatatc acgcttattg gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg
     5041 caccgttacc gcactgcgcc acttcactgc catcagcatt gaaaatgcga tagtgaaaat
     5101 ccagttcagg atcatacggc ggctcaacca ccagcagctg gtcaaaccct acccccaggt
     5161 gccgatccgc caggcgacga atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt
     5221 cgacgaccat aaaatcgttg ccaaggccat gcattttcga gaactgcatc atttactcca
     5281 atcacgcggg tacagaaact gacttttagt aattcacctg ggatggacca tcgccagtgg
     5341 cgcgatcgtt tttatccggc accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg
     5401 gcggtgcgtt tttatctgca ggcgggaaat agagcggacc tttcagaccg cagcccgtca
     5461 ggctgaagag agtaagtaat acagtgagtg ccttaaacac gtttttcatt atggattgcc
     5521 tgtaaagttc atcgctttct gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt
     5581 tagcgactct ttatgaagtc tgaaaagtga ggaaatgctg cgagtataac gcttactcgt
     5641 gaggatttat gttctgctgg cagtttcgtg atcaacacct cgcgccagga tggctttatg
     5701 cactatcgca atgtggtttg tttgttgtct tgttcattgt ttttatcgtc ggcatgggga
     5761 tgtcggcttt ccgcacctga acataatatt taccagaaac agggaaaagg cgtggtgtac
     5821 ctgcgccctt ataaagaaac aaatctttct ctacctgacg tgaattacaa gagtttgcgt
     5881 cggttaccca atttattaat agacccgaca acactagacg aatgggataa agaaccgcct
     5941 ctgactgatt tgacaaccga ctacctctat gagggcgcac aggcctggta tcctcattat
     6001 tcatggcata gcgatggtcg caatatcctc tacgcgggtg aagttgtgca aaaccctccc
     6061 ggcacacctc ccgtcgatgt cgcctcgttc aaggcatggg gtgattttgc cgcagataaa
     6121 cacagtctct attttgaagg caagcgcacc gatgacaatg gtggtggaaa tagcttggat
     6181 atcaaaacgc tgcatcaggt ggaattccgg cctccctggg atcctgactt actggggctg
     6241 atattacgtg acgctaattt cttgtatatc aatgggcatc gcctcgctga ccccgagagt
     6301 ttccgtgtgc tggcgcagaa gtcctgggat cagcgaggaa agttttccac cacgtttaac
     6361 ccctgcattg ccgtaccatt tggcccctgg gataccctgg ctcgtacacg gaccaaaatc
     6421 ctgctcaacg gcgaacagct tgatgccgac ccggacacct tttccgtcgt gcgctggatg
     6481 cccggctcac tcttgacctg gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag
     6541 gaaaatctgg cgtgggatga agatttaaca aagcactgtc tggatttttc tctgctggaa
     6601 aagaaagtgt tctggcgtaa aggacctgct tgtaaacagg aagaattacc cggactcgat
     6661 ccggagcagt ttcaacccat cagtgatgct gtcgcccagc atcaggactc gctttatacc
     6721 atcatcgaaa cagagtctgg taaccgtaag ctggagatcg tgaaacttga tgatcccaat
     6781 cttattatca acaaacgttt caacgccggg aaacgccacg gctatttact tacgcgtgcc
     6841 gaagggtagc cataccattc cggtttacac gtgtttgaat ctgacggacc gctgatctta
     6901 ctggataacc gttctccgga tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc
     6961 agatggtatg cccgcgataa ccgctacatt tacagctttg atggcgcgca gctctggcga
     7021 taccgcaccg ctgatccgaa acaagttcgc ttaatctgga aggaacaaca ttcgggatat
     7081 gtctatggcg taaattacaa aacgggatat ctggacggaa aaattactga tgacggcgaa
     7141 tttattcctg ccccgcgcaa tgaggcgaca aaatgaaggg tcaaataatc acagttgcac
     7201 tcatcctgtt gggacacaca tttctgacgc ctgctgtaca ggccataggg ttcgactatt
     7261 acaatgatca cggtgttatg tcttacggta aaggttattg gggggataag aaaatcattg
     7321 cccagtttcc ggagatgagc agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa
     7381 cgggttatat accttccgat gaaataagca tagaaaatga ggaataccac tgggtgacag
     7441 acggccgcgt tattctctgg cgtggcaaaa tcgttagcaa tccaccggga acactcactg
     7501 tcgatattgc cagctttcag gctatgggcc gcttcgcggt cgataaatat agcctctatt
     7561 ttgacggaca gcgcaccgaa agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa
     7621 aagctatcga aggtaactct accacgctgg tggatagtaa aaatctctac ttgtccggtc
     7681 gacgtcaggg gagcagcagt gatgttactg tattagaaaa aagatggtgg ggtattaatc
     7741 cacgtcttat gagtgtaaac agaaattcat attccaatga tctgcttatc cgcagtgggc
     7801 agaatattta tttaaatggc gttcacctta cggcgaatgc agactcattt gagataattc
     7861 gctggatacc tcattcactg ctggtttttc gcgacaataa gggtctgcat cgttatccct
     7921 ttggtcaatt atcaggcaaa gcgatacccg tagatgatga cgtctctttt gaagtagggg
     7981 aaagtcgcgt tcgctggcgt aaacagctca cgcccgaccg tcagtggagc aagtggatag
     8041 acctaccagg
//
LOCUS       DAJTRN010000151         7899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTRN010000151 DAJTRN010000000
VERSION     DAJTRN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(113..943)
                     /locus_tag="OO328_003688"
     CDS             complement(113..943)
                     /locus_tag="OO328_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5932102.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1153..2166
                     /gene="intI1"
                     /locus_tag="OO328_003689"
     CDS             1153..2166
                     /gene="intI1"
                     /locus_tag="OO328_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5932103.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2369..2719
                     /locus_tag="OO328_003690"
     CDS             2369..2719
                     /locus_tag="OO328_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5932104.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2845..3405
                     /locus_tag="OO328_003691"
     CDS             2845..3405
                     /locus_tag="OO328_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5932105.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3408..6374
                     /locus_tag="OO328_003692"
     CDS             3408..6374
                     /locus_tag="OO328_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5932106.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6441..6818
                     /locus_tag="OO328_003693"
     CDS             6441..6818
                     /locus_tag="OO328_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5932107.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7019..7678)
                     /locus_tag="OO328_003694"
     CDS             complement(7019..7678)
                     /locus_tag="OO328_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5932108.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1752 a         2236 c         2133 g         1778 t
ORIGIN      
        1 gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca aaagcttgct
       61 tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt ttttataaat
      121 ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat gttaaattcg
      181 accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat ttgcttataa
      241 taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct gcaccagttt
      301 tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct atggtgtttt
      361 ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg aattgaattt
      421 gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat ccgttgtttc
      481 tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga taattcttga
      541 ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca gaaaattgca
      601 tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg gttttatccc
      661 atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt gataatgcga
      721 tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg aattgagcaa
      781 tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct tcaaataatg
      841 gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata atatttgcaa
      901 ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt attggtttgg
      961 taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc caactttgtt
     1021 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     1081 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag
     1141 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     1201 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct
     1261 tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
     1321 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
     1381 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
     1441 gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg
     1501 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
     1561 gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact gcgggtcaag
     1621 gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc caaggatcgg
     1681 gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc gcgtgcacgg
     1741 gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc cgacgccctt
     1801 gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt tgcgcagcac
     1861 acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta tgaccagacc
     1921 tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc cgccacaccg
     1981 cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga cattcgaacc
     2041 gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac gcatgtgctg
     2101 aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct cactagtgag
     2161 aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa ggccatcggt
     2221 gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg ccggcagcag
     2281 cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     2341 tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg tggaaacacc
     2401 cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt tcctgttcgc
     2461 caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga acaccaccat
     2521 gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc cgctcgatgc
     2581 cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg agtgctatca
     2641 gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg acgcttgtga
     2701 ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg tgtaaattgc
     2761 gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc atagagtctt
     2821 ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat cagggtcagc
     2881 accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg cgcttttagc
     2941 gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat aagcttcgcc
     3001 cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa tctcgatgat
     3061 ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt cgtcaaggaa
     3121 cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc ggtgatgggc
     3181 gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg tattgcgctc
     3241 gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga gcgtattgcc
     3301 gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg tgaattcgga
     3361 atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg ccacgtcgtt
     3421 ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc aaggacgacc
     3481 tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag cggcgcgggc
     3541 cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc ggcgtcatcc
     3601 tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac cagctcaagg
     3661 tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc gagcacctga
     3721 gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac cggcaggccg
     3781 tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg gccagcgcct
     3841 tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc gtcgagcggg
     3901 cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg gccgaaccac
     3961 tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac aacggcaaga
     4021 cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg catatgctgg
     4081 aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc atcgagcggc
     4141 tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg acacccgccg
     4201 acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg gccaccgagg
     4261 gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg ggtaagctgt
     4321 ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag gccatcaacg
     4381 ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag caatcaggcc
     4441 gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc gagagcgtca
     4501 ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc atcggcgaga
     4561 gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag ctgcgggccg
     4621 cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg aacaccgaca
     4681 acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc tggcagaaac
     4741 tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg ctgtccgagt
     4801 tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag ttcaaggact
     4861 tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc agcgaattgc
     4921 cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg ctgctggaag
     4981 cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat gccatcatca
     5041 ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc gcgcaggcgc
     5101 tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg ctgctcgaag
     5161 tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc gatctggcca
     5221 aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg ggcctgacca
     5281 agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg caagcctggc
     5341 atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct cagtttcggc
     5401 atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga cagaatttcc
     5461 gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc agcagcccag
     5521 gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc aaggtggtca
     5581 atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac gaatccgacc
     5641 tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc ttcgccctga
     5701 tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac accaagctct
     5761 acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc ggcacgctca
     5821 acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc tcgatcaagc
     5881 agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg cgccagaacg
     5941 gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc atcctcgact
     6001 ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag ggcgaggcgc
     6061 gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt gaccgcagtt
     6121 tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc atcgtgctgt
     6181 ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt catgccgtcg
     6241 atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac ctgaccggtg
     6301 attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg ctacggcctc
     6361 tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat tatggcgaag
     6421 gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc aaccggcttt
     6481 ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg gcatgttgca
     6541 atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag ctgtatgttt
     6601 ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata gcccggcttg
     6661 cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac cccggcgcta
     6721 accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat tattacctcc
     6781 acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt
     6841 ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc
     6901 cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat
     6961 cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaatt
     7021 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     7081 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     7141 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     7201 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     7261 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     7321 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     7381 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     7441 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
     7501 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     7561 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     7621 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     7681 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     7741 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa
     7801 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt
     7861 gatcttccgt cacaggtatt tattcggcgc aaagtgcgt
//
LOCUS       DAJTRN010000152         7726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRN010000152 DAJTRN010000000
VERSION     DAJTRN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO328_003695"
                     /pseudo
     CDS             complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO328_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            763..915
                     /locus_tag="OO328_003696"
                     /pseudo
     CDS             763..915
                     /locus_tag="OO328_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2296..3243
                     /gene="icsP"
                     /locus_tag="OO328_003697"
     CDS             2296..3243
                     /gene="icsP"
                     /locus_tag="OO328_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5932109.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3805..4177)
                     /locus_tag="OO328_003698"
                     /pseudo
     CDS             complement(3805..4177)
                     /locus_tag="OO328_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4421..4540
                     /locus_tag="OO328_003699"
                     /pseudo
     CDS             4421..4540
                     /locus_tag="OO328_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4790..5656
                     /gene="ospB"
                     /locus_tag="OO328_003700"
     CDS             4790..5656
                     /gene="ospB"
                     /locus_tag="OO328_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5932110.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5985..6725
                     /gene="phoN2"
                     /locus_tag="OO328_003701"
     CDS             5985..6725
                     /gene="phoN2"
                     /locus_tag="OO328_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5932111.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7085..>7726)
                     /gene="ospC4"
                     /locus_tag="OO328_003702"
     CDS             complement(7085..>7726)
                     /gene="ospC4"
                     /locus_tag="OO328_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5932112.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYK
                     VLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2419 a         1449 c         1448 g         2410 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaatctctg gcgactcatc tcttaacact
      181 tgcataatct ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct
      241 tgggaagaat catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttagag
      301 aagatcttac aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa
      361 ctatccacag aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct
      421 tccggtgtac tggcttcggc tctggaaata ggctgaaaat cttccggaca tggagaatac
      481 attgtcttca tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat
      541 tgtgccatac ttctggttat accagagata tacttcttgt catatatgtt tggtgattca
      601 ttaaagtaac catgttcaac tctaggataa atatttctca ctatatattc agtatttgca
      661 gtatttattg gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata
      721 ctcaaatata aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga
      781 gaacacttta tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcaat
      841 tacaatcgct ggcgacggca ccgttggtgt ggcggcctca gtccggaaca atttgaaaac
      901 cagaacctcg cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac
      961 gactgcgtcc atattatgtg ggtaggatca ataaagacgt tttatatgtg tctgcttgca
     1021 gccagaaaat ttataccctg caggtatcat aaacatgctt gataacttaa ttggggctcc
     1081 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     1141 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagcttat
     1201 ccaaactgcc aagcctcttt attataagta agatctggca ctggaatatt ctcaaaagga
     1261 ctaacatcat caccacgacc ttcaaccaac cattttattg catttaaatc ccagaatagt
     1321 tcagtagtgg tcaatttggg aaacgcacaa tattgtacgg ttacgttatc gcaatagagt
     1381 gaactctcgc cctatacggc gttttcaagg attagtcctt aatcggacaa ccacattaaa
     1441 gcataataaa taattaagct ttaataatat actatagata cagttctatt ataaaaaata
     1501 ctattttagg tgatgcatta aattagtaga atatttattc ttttataaaa gcaccaatga
     1561 attgtccaag taaacatgag ataaaatgtg tagaggataa ctgagtttgg tcactttaac
     1621 tgtattagtc gcagtctgac tctcgacttt aaaaggatgg gaattacctg tactgatagt
     1681 gagaaagctt tccattgaaa taagtcaaat aaaagttgat ggcttcagtg ctaaagtgag
     1741 gtgcccgcta gccgtattca ctcgaatcgg aattcaatgt attgcttctg ccatttccat
     1801 aatatttcac gggaaaaaca aaatctgaaa aactatactt aagtgttgta ttgagtcagt
     1861 tgagatacaa atacatattt atcagcacac gttacgtcaa atacaggagg ttgtcatgtt
     1921 ggtactgaaa ggcacgttgg ctattgcagc cgtgatgaca atttattgtc ttatcgttgt
     1981 tcttatggaa cgcttttctg attgatttca tattggcgag agaacaagaa ggtgagtaga
     2041 attgtcgcgg aatcctgaaa ctatcagcct caggtatgaa caccaaaagg taggtagaga
     2101 aatctatggc ccccgttact ttattcgcac aatgaggtat ttttttattt ttacaaaagt
     2161 tcaataaata ttataataaa agatatgttc ttggtaacat aaacaaatat ttaattaaaa
     2221 taatgcttgg cattgacgtt tatcttataa agtaagaaga tcatggacat ttcaaccaaa
     2281 aaagtagagt tctcgatgaa attaaaattc tttgtacttg cactttgtgt acctgcgatc
     2341 tttactacac atgctaccac taactatcca cttttcatac cggacaacat cagtacggat
     2401 attagtctag gatctctgag tggcaaaaca aaagaacgcg tttatcatcc caaggaaggg
     2461 gggagaaaaa ttagtcaact ggactggaaa tacagtaatg cgactattgt tagaggtggc
     2521 atcgattgga agctaattcc aaaagtgtct ttcggagttt ccggttggac tactttaggt
     2581 aaccagaaag caagcatggt tgataaagac tggaacaatt ccaatacccc tcaggtatgg
     2641 accgaccaga gctggcatcc caatacgcat ctccgtgatg ctaacgaatt cgagctgaat
     2701 cttaaaggtt ggttattaaa taatttggat tatcgacttg gactaatagc aggttaccaa
     2761 gagagtcgtt acagttttaa tgcaatggga gggagttata tttatagtga aaacgggggg
     2821 agcaggaata aaaaaggggc acatcctagt ggtgaaagaa caataggtta caaacagctc
     2881 ttcaaaatac cttatattgg attaactgct aattaccgcc atgagaattt tgagtttgga
     2941 gcagaactga aatatagtgg ttgggttctt tcatctgata cagataaaca ctatcagact
     3001 gagacaattt ttaaagatga aataaaaaac caaaattact gctctgttgc tgcgaatatt
     3061 ggatactatg tcacccccag tgcaaaattt tatatagaag gctccagaaa ttacatttct
     3121 aataaaaaag gtgatacatc tctttatgag caaagtacca atatatctgg caccattaaa
     3181 aatagtgcaa gtattgaata tattggtttt ctcacttccg caggtataaa gtatattttt
     3241 tgaattggtt caccgagttg attacgtttt catggctaaa aattgccgcc cattgtgagt
     3301 catattcgcc ctgactttcc agaaccatac gaactgcccg ttgacggact tcgggggaaa
     3361 aacgtgtatt tttagtcatt ctgtttacct ctttctcagg gcgtttagtc tccaggattt
     3421 ccggggcggt tcaaaaaact cttcgttgat tactgtggac ttactgttgg cattaccgac
     3481 cctgagaccg gagaaataag aaccgcttag atcatcgtgg ctgttctcgg ggtatcaagt
     3541 tacacatggg cagaggctac ccaaggtcta ctattatcgc ctgaaagcgc tgatggaaca
     3601 gtgctatcag ggacatgctg atgaaaatac ctaaataatc agccagcact ctatctttcc
     3661 aaatccacag catagcaaag agagcaaaag agcctgtaaa ttcagaaatt aaggaaacgc
     3721 ctcttggtgt acataatcta tcttatgtta aatgaaatct aatttcttga ttatatgctg
     3781 gatcgttcgt gggtggcttt gcccctatat ttccagacac ctgttatcat tggcccgctg
     3841 ccgcagatat tcccgtggcg agcgataatc cagcgcacta tgcggatgcc attcgttata
     3901 atgctcgaat gcctctgcaa ggttctttgc tgccgttaac ccgtcaggtt tgggcatgat
     3961 actgatgtag tcacacttta ttgttttcac gaagctcttt gctattctgt tactctccgg
     4021 actccgcacc gccgtgttct tcggttcaac aaagtcccaa catccgggtg aactggcgtg
     4081 tttcattagc tcggtagcat gaaccattat ccgtcagtca cttcactgga gacgccggaa
     4141 gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc gccaacggga gagcatctac
     4201 ggtctgattg aactgtttat tcaaattgtc caccgtctgt cagtacgctc tgaacgtcgg
     4261 ctggttaaaa cactgctcgc tgattttcag agggttcacg gtaaaacagc acttttattc
     4321 cggattgcgg aagcggctct gaataatccg gatgggctgg ttaaagaggt tgtgtaccat
     4381 ctgcatattg ttgaaccaga tcgcagcggt aagagaagtt catcatactt ggcgcagtta
     4441 cgcgatgttt ctgctagagg tgatgccgta aagaacggca gaacattacc ggaacaagat
     4501 agcggtctac ctgcactggt atcagacccg gggcttcccc ggatgatctc cacggtactt
     4561 taggtactgt gtctatatta tgtaggtagg atcaattaac tttgtagttg aaattctttc
     4621 attgaaataa tgaataaaaa agacaaaccg cattaaacaa tgccagaata taattgcatt
     4681 tttgttccgt ttttttaaat catagtatac aaagaagctt gcatactata tgaaaatata
     4741 acacccttta ctatgatgtt tataaaacaa tatatggagt catgtaggta tgaatttaga
     4801 tggtgttaga ccatactgta gaatagtcaa taaaaagaat gaaagcatat cagatattgc
     4861 atttgcacat ataataaaaa gggtaaaaaa ttcatcatgt actcacccaa aagcagcatt
     4921 ggttttttta ggagagaaag gtttttgtga tagcaatgat gttctatcta ttatgggaca
     4981 acaaatacca agagtattta agaacaagat gttatatgat tatgttttta aaaatgaaaa
     5041 aagtaaaaat gattttctaa aaatggctga atcatggcta ccacagagtg aaccaatagt
     5101 aataaataat gatgatgacg cattgaatgc tgctgcttat ttttctgtaa aaaaagcgaa
     5161 aataaaaaca gtaaacgata ctgattttaa agagtataat aaggtttata ttcttgggca
     5221 cggtagtcct ggttctcatc aattaggcct tggttcggaa cttattgatg tacaaacaat
     5281 catttcaaga atgaaagact gtggtattct aaatgtgaaa gatatccgtt ttacttcatg
     5341 cggctccgct gataaagtgg ctcctaaaaa ttttaacaat gcccctgctg aaagtctttc
     5401 ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa tctttgctag agcagataaa
     5461 aaaacacctt gaaaacgatg agtcattgag tgatggtcta aaaatatccg gctatcatgg
     5521 atatggagtt cactatggtc aagagctttt tccctactca cattatcgtt caacttcaat
     5581 tcctgctgat ccggagcata cagtaaaaag aagctctcag aaaaagactt ttattattaa
     5641 taaagaactg gattagtata aaatttttaa cctatagtta attgtttgca tttaagatga
     5701 agttatttta tctttagtag aaaagtgctt ccctatgagg gaaaatcaca aatcatcata
     5761 atacagagac aaaacgatac gaaaaaatag ataaaaaaca tcattctttt accacattat
     5821 tttccgtgaa tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc
     5881 atcactctgt tcaaattttt ccgcatgact tgtgtttttt gtaatacagc tcgtttttta
     5941 cagctgacca aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact
     6001 ttcttctttt ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg
     6061 cagaaggttt tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc
     6121 cggcagagaa ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc
     6181 tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc
     6241 ttgcattttc agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc
     6301 agttgttaac acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat
     6361 attataaaag agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag
     6421 atgagaaaat ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag
     6481 tagcactgat acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat
     6541 atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg
     6601 ggcgtttaat gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa
     6661 gccttagcga agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc
     6721 cataaagctg gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat
     6781 gagcatagga ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg
     6841 aaaggctggg acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa
     6901 acgatgatct ggcaacctga gttcacagat aaaacattct ctaggaaact cggggcggtt
     6961 ccgttcacca catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga
     7021 tcaatataac agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca
     7081 cctctcaaat cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg
     7141 aatctttgct tttcattgca ttgtcaagca tagtgtcccc actatttgct ttttgaaatt
     7201 ttttgtttac atcaaccaat ccattattga taaaatattc tagcacttta tagttggcac
     7261 catctttact aagtaagtat tcaacatcat acagattaca atatctattg tttttcattt
     7321 tctccatctc tgcgacatct tgttttgtaa aattaaattt agatagtaaa taaagtgcta
     7381 tatcagcttt tttattgcct aaagaataag caagggcctg atgagccatt tttttatcag
     7441 tcactttatt gttaatctct tcataattag atgctttaat cgcatcctcc aaagatatgt
     7501 ctctgagttt tatttttttg aagttatctg tacttagcat tcttggtata tgatactcaa
     7561 gctgaaacac aaagtttata attctatcca gactaacagg atcaatattt ttgttataac
     7621 aaaactctct gaatccttga tctttactct ttcttataaa atcaataaga tgcaacccaa
     7681 ttccaagcct catatcttta gtgttaaata ttggaacatc agtttt
//
LOCUS       DAJTRN010000153         7546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRN010000153 DAJTRN010000000
VERSION     DAJTRN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..1057
                     /gene="yjeM"
                     /locus_tag="OO328_003703"
                     /pseudo
     CDS             1..1057
                     /gene="yjeM"
                     /locus_tag="OO328_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1208..4519)
                     /gene="mscM"
                     /locus_tag="OO328_003704"
     CDS             complement(1208..4519)
                     /gene="mscM"
                     /locus_tag="OO328_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5932113.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4541..5509)
                     /gene="psd"
                     /locus_tag="OO328_003705"
     CDS             complement(4541..5509)
                     /gene="psd"
                     /locus_tag="OO328_003705"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5932114.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5606..6658)
                     /gene="rsgA"
                     /locus_tag="OO328_003706"
     CDS             complement(5606..6658)
                     /gene="rsgA"
                     /locus_tag="OO328_003706"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5932115.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6753..7298
                     /gene="orn"
                     /locus_tag="OO328_003707"
     CDS             6753..7298
                     /gene="orn"
                     /locus_tag="OO328_003707"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5932116.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1712 a         1973 c         2041 g         1820 t
ORIGIN      
        1 cagtcgttgc ttcaaagggg attaataaaa ttgcccgcat tactgcggtg ggcggtattg
       61 cagtaatgtg tctgaattta gtattgctgt tagtaagcat tactattttg ttattaaatg
      121 gtgggcattt cgcgcaggat attaatttcc ttgcatcacc gaacccgggt tatcagtccg
      181 gtctggcaat gctatcgttt gtggtatttg ctatttttgc ctatggcgga attgaagcgg
      241 ttggtggtct ggtcgataaa acggaaaatc cagaaaagaa ctttgccaaa ggtattgttt
      301 ttgccgctat tgttatttca atcggttatt cgctggcaat atttttatgg ggcgtcagca
      361 caaactggca gcaggtatta agtaatggtt ccgttaacct tggcaatatt acctatgtgc
      421 tgatgaagag cctcggggtg atgctgggta acgcactgca tttgtcacct gaagcgtcat
      481 tgtcgctggg cgtatggttt gcgcgtatta ccggactttc gatgttcctc gcttataccg
      541 gtgcgtttgc tattcaccgc tgaaagccat catccagggg acgccgaaag cgttgtggcc
      601 ggaaccgatg acgcgcctga atgcgatggg gatgccgtct atcgccatgt ggatgcagtg
      661 cgggttggtt actgtcttca ttctgctggt ttcgtttggt ggcggtaccg catcggcgtt
      721 ctttaacaag ctgacgctga tggcgaacgt gtctatgacg cttccttacc tgttcctcgc
      781 gctggctttc ccgtttttta aagcacgtca ggatctcgac agaccatttg tgattttcaa
      841 aacgcgtatg tcggcaatga ttgcgacggt ggttgtcgtt ctggtggtga catttgcgaa
      901 cgtcttcacc attattcagc ctgtggttga agccggagac tgggacagca cattgtggat
      961 gattggcggc cctgtcttct tctcgctgtt agcgatggcg atttaccaga actattgcag
     1021 tcgcatggcg aataaacctg agttagctct cgactgatca tatgaaatcc tgcaactcat
     1081 cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg gatgatactc tccagggccg
     1141 gtgataactg aagatgatgt taagctgcta aatgagccac gttgacatca tttggcagct
     1201 tcatgcatta caaacttccc gcctgacgac ctttgcccgc agacgtcagc gttctccccg
     1261 tttgtttacc gttgaggctt tccagacgca tctggaaggg cgggaatggc atatcgatac
     1321 cgtgggcgtg gaagcctgcc agaatcagct ggtggatctc atggcgtagc ggcatacggt
     1381 gacccatctc agcggcgtaa atacgcagct cgaaaatctg aatcccctgt tgcagatcca
     1441 ccaggaagac ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc gctgcggtga
     1501 gcaggatttc cgtcacttct tcactgttgg catcggcagg ggccggaatc gtcaacacta
     1561 cgcgcgtgac cgagtcagag agcgaccagt tgataaactg ctcggtaata aacgccttgt
     1621 tcggcacgat tatctcttta cggtcccagt cgctgatggt ggtggcgcgg gtgttaattt
     1681 ttgtcacgct accagtaaga tcgcggatcg ttaccgtatc gccaatgcga atcggttttt
     1741 cgaacaggat gatcaggcca gagataaagt tggcgaaaat ttcctgcaaa ccaaaaccga
     1801 gaccaacacc gagcgcggca accagccact gcaatttcga ccactcaata ccaatcattg
     1861 agaagccgac cagcccgcca atcagcatca gcagatattt tgtgatggtg gtgatggcgt
     1921 aacccgtacc cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc agcgcgggca
     1981 ggttgcgcac cagctgcgtg gtgatgataa ataccagaat ggcaatcagc accgcaccga
     2041 gggtaattgg ctccagactt tctacgccct gtaccgtgga ggtgacatcc cacagcgaaa
     2101 tattttcgag gaagccgaaa gcggaatgga tttctgacca cagcacaatg accgaaagca
     2161 gggcgatcaa catcaaaatt gagcgcacca gccgcaagga ttgcgcactg atggcatcga
     2221 gatcgacttc gctttcatcg acttcaattg ctccttccgg gctactgtga tgatgcgctt
     2281 cctcttcgcc acgcgcacgt tgcgctaaca tctctgcccg gcgatgcttc gcccggtcaa
     2341 acgccagcct gcgacgctgg atcagcatcc agcggcggat aacgtgataa accaccagta
     2401 gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa cagtgcctgc gccgttgcca
     2461 gataacccac cgccgacgcc agaatggcaa ccagtggtgc gccaatcatc atgttccaca
     2521 gcatatggtt ggtaatgttg tcgccgctgc cttctttgtt gagatacaac ggaataccgg
     2581 cttttttcag gcttagggtg accaccgcca gcgcgccgca aatgaggata aagcaaagcc
     2641 gtcccagcga accggaaaat tcgcggtcgt cgaggttatc gaacatcatc agcgccataa
     2701 tcagcccgat gctcatcagg tagtagcgca tcccacggga aacacgttcg cgcggccagc
     2761 caaaatgagc gataaacaag ccgttcgggc gggcaaaggt ggcgcaaatc atcactaccc
     2821 acagcagcgg cacggtggcc gttacaccat cgccaatcgc gaccgccagc ggataaggcc
     2881 acgcctcgcg caagccgtaa cccagcgtca tccacagcac cggtaacggt gacgcgacga
     2941 gaatcgacca gaaaagagtg cgcaacgtca gccagaagtg atcctgagtc actttgccga
     3001 ctttcgccgc cgaacgttca agaaaacggg tgaaatagcg gcgcgagtaa atactgcaac
     3061 cgaccagaat caacgcgcca aacagcggca aaattgtctc tttgctggtc agcatcatca
     3121 cactggcttt gcccaactga ctgaaggtgt ccagcgaaat gagacgacgc agatcctggg
     3181 cgatttccag cggccaggcg atggtcatcg ggcgcacgtc agaggcccag aacagatagc
     3241 ggtgcgttgc ttcgttcacc tctttcagcg catcctccag ttgcctgttg gagactttca
     3301 gcttggtcag ttccaatagt agcgtgtcgc caccctgcaa caatgagttc agcaactcac
     3361 gctgagtgcg cagttgtgct tccagaatac ggttttgctc ggcggtcagc ggctgaccgt
     3421 cggcctgatg gatttgccgt agcagcggct gtttattaag taaatcctca taacgtaacc
     3481 gttgcacacg caactgcgcc atttcggtat caagctgttg tggttttggc atttctggta
     3541 gccgtgccac ctgcgcccgc agcgcttcgc cgagcaggtt ggacgatccc agccattgcg
     3601 actgttcacg cagcgtattc aacgcctgcc ggacctgtaa cgtctggctg gcagcctgac
     3661 gctgttgcga ggcaacgagc tccatccgct gcgcctgttg attcagcgct gccgatagtt
     3721 cgcggttaat tttgaattgc gcgacgatat ctttcggcaa atcggcactg ttttctgcca
     3781 gtaattcggt actttccagc gcccgctccg cctcaagctg acgttggcta ttcaactgat
     3841 tacgtaaggc ctgcaaatac gcatccagtt gctggctctc tttttccgcc agctctgagc
     3901 gtaagcgcgc taattcctgg cggttattgg cagacagctg cgccagctcc agttcatcaa
     3961 cgagcgcctt aagacgtgca gagtcagact gcaacgcgaa attttgtgcc tgattgagcg
     4021 gagtattgcc ggtaagcgtt cccaggcggc gctcgatctc atttaactga cggcgggcgt
     4081 cggtttgctg ttgcggcagt tgattcagcg aatcggcaat ctcgcgggcg cgctcctgct
     4141 cttgctgggc ctgacggctt ttatccagca actggctgct gacctggaga atttcctgat
     4201 tcagcgcgtc ggtagacatt cccggcgaca cgctgcgcgg ctcgtcacga atgttgttta
     4261 attgtgcgcg cagagtagcg gagagtttcg gataattatc gataacttgc tgatattgtt
     4321 tgatgcgctc aagggaacct tttcgttcct caagcgcatt taaggcagac tggagcgcct
     4381 ctacgacttc cggctgtgcg ggtttcgccg cttttgcctg ctccagttcc tgagtgattt
     4441 gtttgctatc gggggccgtc gcggcgtacg ccccccaact gaggcaccag gccatcagaa
     4501 aagtgataat caggcgcacg tcagcgtttc ctttgatgga ttagacctgg tcttttttat
     4561 cgtcaaccaa tgggctggcg tcgtgttctg cttcgatctc ttcagcagga agtggggcgg
     4621 gttcagcgtc tggcgtaaca aaggtttcgg tagatactgc cagcggctgg ccaattttcg
     4681 tgacagacag gctttccagt tgctcaacca gattcacttt acccggtgca aacaggttga
     4741 taacggtgga accgagttta aagcgaccca tttcctggcc tttcagcagt gccacagaac
     4801 cgtcgttttc cccggcaggc caggtccagc gcttgatgat accttcgcgc ggcggcgtaa
     4861 tggtgcccgc ccagaccgtc tcaatgctgc caacaatcgt cgctccgacc agaatctgcg
     4921 ccattgggcc aaattcggta tcgaaaaggc aaatcacgcg ttcgttacgg gcaaacagat
     4981 ttggcacgtt ctgagcggtg agatggttaa cggaaaagag atcgcccggc acgtagatca
     5041 tctcacgcag aataccgttg cacggcatgt gtacgcgatg gtagtcacgc ggggagaggt
     5101 aagtggtcac aaacgtaccg ttgcggaaca ggtccgccat cagatagttg cctgccagca
     5161 gggcttcgag gctgtagttg tggcctttgg cttgcaggat tttatcttct tcgattttac
     5221 ccaactggct gataacgcca tcggcaggca tgaccagtac attcggatcg gtatcgattg
     5281 ggcgtacttc gtcacgcagc ggacggacaa agaattcgtt aaaggtgcgg tagctggcgg
     5341 tgtccggctt ttgcgcctct ttcatgtcga ccttgtagta tttaacgaac agatcgataa
     5401 ccagttttgt cagccatcct gcccgcttgc ttgcgcccca acccgccagg cgagtaagcc
     5461 atagtttcgg cagaatgtac tgtagcgaaa gtttaaatga atttaacaag gtagcctcca
     5521 ggccattgtt ttgtcgttcc tgatccggcc aacatgccgg atcctgaaaa aaaggggacg
     5581 attctaacga cggttagctt aattgtcagt catccgtatc agaaaagttt ttacgcgttt
     5641 ttacctgcgc catgctttcc agaatacggt gatagttttc gaaacgggtt tccgcgattt
     5701 tcccttcctc aaccgcttcc cggatagcgc agcccggatc ggtatcgtgt ttgcaatcgc
     5761 gatatttaca cagacctaaa tagtcatgga attcgacaaa gccctgagtg atttgttccg
     5821 gctccaggtg ccagaggccg aactcacgca caccagggga atcaatcaca tcaccgccgt
     5881 gcgggaagtg atacagccga gcggcggtcg tggtgtgctg accgagcccc gagttgtcag
     5941 agacatcgtt ggtcaggatc tctttttgca gccccagcaa cgcgttcagc aggctggatt
     6001 taccgacgcc agactgcccg gcaaaaatgc tgatgcgccc ggtcaacgcc tcttccagcg
     6061 gtttcagccc atcctgagta tggctggaaa ccatcaatac gcgataaccg atgttgcggt
     6121 agatatccat ctgctcgttg acgaacgcca tgccttcgtc gtccagcagg tctatcttgt
     6181 tgagcacgat aatcggctca atctgcaagg tttcgcaggc caccaggtaa cggtcgataa
     6241 tattgagcga cagctccggc aaaatggcgg agacaatgac aatctggtcg atgttggcgg
     6301 ctatgggttt cacgccgtcg tagaaatccg gacgcgttaa caccgaggtg cgctcatgca
     6361 ccgcttccac gatccctttg acatttacgc cttccgccgc cggtttaccc ggacgccaga
     6421 ctacgcggtc gccggttacc agcgaacgga tggtacggcg aatattgcag cggtgaacgt
     6481 cgccatcggc ggattccaca tcagcgtgca taccaaagcg gctgatgacg ataccttcat
     6541 caggctcgcc aaacagattg tcgtcgtagt cgggcttctc cttagacgtt ttaagacgac
     6601 gctggtgatt ggcgttcacg cggcgctgct ggcctttgga gagtttattt ttactcaatc
     6661 gtactgactc ctggtcgccc ctgatgggca aaacatctat gatacacgca attgtggatc
     6721 aatatagtca ctgtgaatgg gtggaaaata gcatgagtgc caatgaaaac aacctgattt
     6781 ggatcgatct tgagatgacc ggtctggatc ccgagcgcga tcgcattatt gagattgcca
     6841 cgctggtgac cgatgccaat ctgaatattc tggcagaagg gccgaccatt gcagtacatc
     6901 agtccgatga acagctggcg ctgatggatg actggaacgt gcgcacccat accgccagcg
     6961 ggctggtaga gcgcgtgaaa gcgagcacga tgggcgatcg ggaagctgaa ctggcaacgc
     7021 tcgaattctt aaaacagtgg gtgcctgcgg gaaaatcgcc gatttgcggt aacagcatcg
     7081 gacaggaccg tcgtttcctg tttaaataca tgccggagct ggaagtctac ttccactacc
     7141 gttatctcga tgtcagcacc ctgaaagagc tggcgcgccg ctggaagccg gaaattctgg
     7201 atggttttac caagcagggg acgcatcagg cgatggatga tatccgtgaa tcggtggcgg
     7261 agctggctta ctaccgcgag cattttatca agctgtaaaa tttttcctgg tcacgtaagc
     7321 gccggaatcc acttgcggcg gcgtgatttt gacgctaaat cgaacgtctt gctgataatt
     7381 tcagcacttg agataaaaac gcaaaaaaaa cttttttggg gggttgcaga gggaaagatt
     7441 tctcgtataa tgcgcctccc gtaacgacgc agaaatgcga aaattacgaa agcaaaatta
     7501 agtagtacgc gggaatagct cagttggtag agcacgacct tgccaa
//
LOCUS       DAJTRN010000154         7464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRN010000154 DAJTRN010000000
VERSION     DAJTRN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..1159
                     /gene="rbsA"
                     /locus_tag="OO328_003708"
                     /pseudo
     CDS             1..1159
                     /gene="rbsA"
                     /locus_tag="OO328_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1164..2129
                     /gene="rbsC"
                     /locus_tag="OO328_003709"
     CDS             1164..2129
                     /gene="rbsC"
                     /locus_tag="OO328_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5932117.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2154..3044
                     /gene="rbsB"
                     /locus_tag="OO328_003710"
                     /pseudo
     CDS             2154..3044
                     /gene="rbsB"
                     /locus_tag="OO328_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3170..4099
                     /gene="rbsK"
                     /locus_tag="OO328_003711"
     CDS             3170..4099
                     /gene="rbsK"
                     /locus_tag="OO328_003711"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5932118.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4103..5095
                     /gene="rbsR"
                     /locus_tag="OO328_003712"
     CDS             4103..5095
                     /gene="rbsR"
                     /locus_tag="OO328_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5932119.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO328_003713"
     CDS             complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO328_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5932120.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6511..7203)
                     /locus_tag="OO328_003714"
     CDS             complement(6511..7203)
                     /locus_tag="OO328_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5932121.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1892 a         1809 c         1968 g         1795 t
ORIGIN      
        1 aaaccatgta tgtcgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg
       61 acaagttagt gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc
      121 tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg
      181 aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccac tgtattgtct
      241 atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg
      301 atgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga
      361 tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg ccaggagata
      421 tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac
      481 gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga
      541 aagtgctcta cggcgcactg ccgcgcacca gcggttacgt caccctggat gggcatgaag
      601 tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc
      661 gtaaacgtga cggtttagtg ctgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc
      721 tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga
      781 gtgatttcat tcgtctgttt aatgtgaaaa cgccgtcgat ggaacaggca attggtctgc
      841 tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cccccaaagt
      901 attgatcctt gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
      961 actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat cggagatgcc
     1021 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     1081 atttactcgt gagcaggcca cccaggaagt gttaatggct gtcgctgtgg gcaagcttaa
     1141 tcgcgtgaat caggagtaaa aaaatgataa cccagactgt ctctggtcgc cgttatttca
     1201 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     1261 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     1321 cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatag
     1381 acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     1441 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg
     1501 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
     1561 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg
     1621 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     1681 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     1741 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     1801 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     1861 tggccgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg gggactggct
     1921 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     1981 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     2041 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     2101 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     2161 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     2221 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     2281 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
     2341 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     2401 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
     2461 ctaactaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
     2521 tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
     2581 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
     2641 cccgtgaacg tggcgaaggc ttccagctgg ccgttgctgc tcacaagttt aatgttcttg
     2701 ccagccagcc agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga
     2761 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
     2821 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
     2881 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
     2941 atcagattgg cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc
     3001 aggctaagta tccggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
     3061 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
     3121 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
     3181 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
     3241 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
     3301 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
     3361 tggcgatgac agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat
     3421 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
     3481 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgctcttt ccccatcgct
     3541 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagcg ttattaatgc agctggaatc
     3601 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
     3661 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
     3721 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
     3781 tgcagcgaag gcggcgcagg tactgcatga aaagggtatc cgtactgtac tgattacttt
     3841 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
     3901 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
     3961 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
     4021 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
     4081 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgtt tggcgggcgt
     4141 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
     4201 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
     4261 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
     4321 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
     4381 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
     4441 aaaacgcgtt gatggcttac tgttactgtg taccgaaacg catcagcctt cgcgtgaaat
     4501 catgcaacgt tatccgacag tgcctaccgt gatgatggac tgggctccgt tcgatggcga
     4561 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
     4621 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
     4681 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
     4741 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
     4801 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
     4861 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
     4921 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
     4981 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
     5041 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg catagattta
     5101 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
     5161 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
     5221 gtgccttcca tcccttcata aatgcgaagg acggccgcac ttacagcaac gcctaaacta
     5281 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
     5341 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
     5401 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
     5461 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
     5521 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
     5581 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
     5641 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
     5701 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtacgtcg cgcatggaga
     5761 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
     5821 acaatctttt ccccgaatag ctctattccg cttgaaaaga gaacaaggct gaggccaaac
     5881 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
     5941 cgcgcgtaaa gaaggcccgc aatacctatg gggatattaa ttaaaaatat ccagtgccag
     6001 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
     6061 ggcatggcga caaaattcaa taccggaaga agttcattac gaggataagc acgcagtaag
     6121 gccggccgag caacaggcat catcattgcg tcgcctatcc cttgaataac ccggaagaca
     6181 accagctgtg gtagcgaatt agaaagtgcg caggccagag aacccaacgt gaacagactc
     6241 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
     6301 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
     6361 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
     6421 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg acatactacg cttcttttta
     6481 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
     6541 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctggtg
     6601 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
     6661 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
     6721 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
     6781 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc
     6841 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
     6901 atgaaagttc tcttctgtta tccaccaagt aagcaattcc tgatcaagaa aattccagtt
     6961 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
     7021 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
     7081 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
     7141 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
     7201 cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
     7261 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggtg ttttgctgaa
     7321 aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggga taactcccta
     7381 taatacgcca ccactgacac ggaacaacgg caaatacgcc gccgggtcag cggggttctc
     7441 ctgagaattc cgacagaaaa agca
//
LOCUS       DAJTRN010000155         7449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRN010000155 DAJTRN010000000
VERSION     DAJTRN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(28..2940)
                     /locus_tag="OO328_003715"
     CDS             complement(28..2940)
                     /locus_tag="OO328_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932122.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKVFYERSEKSVGYWVGGIRRAAQKA"
     gene            2943..3059
                     /locus_tag="OO328_003716"
     CDS             2943..3059
                     /locus_tag="OO328_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5932123.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3423..4265
                     /locus_tag="OO328_003717"
     CDS             3423..4265
                     /locus_tag="OO328_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5932124.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4268..5356
                     /gene="capU"
                     /locus_tag="OO328_003718"
     CDS             4268..5356
                     /gene="capU"
                     /locus_tag="OO328_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5932125.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5361..6311
                     /gene="virK"
                     /locus_tag="OO328_003719"
     CDS             5361..6311
                     /gene="virK"
                     /locus_tag="OO328_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5932126.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6376..7320
                     /gene="lpxM"
                     /locus_tag="OO328_003720"
                     /gene_synonym="msbB"
     CDS             6376..7320
                     /gene="lpxM"
                     /locus_tag="OO328_003720"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5932127.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2019 a         1642 c         1740 g         2048 t
ORIGIN      
        1 tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc
       61 cacccagtat ccgacagact tttcactgcg ttcgtaaaaa accttcttcc ctcccagcat
      121 atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc
      181 cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg
      241 gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt
      301 gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc
      361 cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc
      421 ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac
      481 cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga
      541 cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg
      601 cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag
      661 caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc
      721 tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat
      781 atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag
      841 taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc
      901 attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt
      961 tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat
     1021 aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc
     1081 ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat
     1141 ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg
     1201 atgctccacc tgctgttcaa caagatacac attgtgttta gcacgggtga gcgcaacata
     1261 cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg
     1321 ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa
     1381 tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt
     1441 aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg
     1501 ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc
     1561 ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct
     1621 tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag
     1681 caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt
     1741 aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga
     1801 gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg
     1861 acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac
     1921 aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact
     1981 gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt
     2041 cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg
     2101 ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag
     2161 ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc
     2221 actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga
     2281 aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc
     2341 tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat
     2401 tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag
     2461 cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg
     2521 gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc
     2581 cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg
     2641 tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg
     2701 gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt
     2761 ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa
     2821 ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc
     2881 tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa
     2941 ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat
     3001 ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc
     3061 tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc
     3121 cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agtacctgtg ttgttctgag
     3181 gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt
     3241 gagtcgtttt taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt
     3301 agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata
     3361 aaatgcacca gctttattgg gttttccttt tgtctctgct tgctaggtgc cagaaacttt
     3421 aaatgttaaa cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc
     3481 atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa
     3541 taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt
     3601 atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg
     3661 acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt
     3721 taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc
     3781 atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt
     3841 ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc
     3901 atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg
     3961 acttgttgag cgggagggaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt
     4021 gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct
     4081 attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag
     4141 gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt
     4201 atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg
     4261 attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca
     4321 ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag
     4381 ggaaaacagc aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt
     4441 atttcgaaac agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt
     4501 tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg
     4561 gttattcact aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa
     4621 aacaaaagtt ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat
     4681 gaagacacat ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt
     4741 tgatttccag aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg
     4801 gctggcgtcc cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa
     4861 agggcacgaa tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg
     4921 ttggttgatt gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga
     4981 cagtatgggc atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt
     5041 atatcgggtt gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact
     5101 ggcagaagca tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga
     5161 tgttattcag aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat
     5221 gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc
     5281 aaaacaggat atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt
     5341 agcgaagcac aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta
     5401 aaaggattgt tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt
     5461 tcatgtacat tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac
     5521 tgcgttcctt gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa
     5581 ttcatcgacc ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg
     5641 agcattatcg ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa
     5701 aatcggtatc attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt
     5761 ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag
     5821 tgattgcccg gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg
     5881 gcggattaca gggagccccc aaaaataccg gacctgatat tattcggtgc gcaacccggg
     5941 cctgttatgg gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag
     6001 cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga
     6061 gatactggta tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg
     6121 tagcagggaa aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc
     6181 taagcaatat agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt
     6241 atatccatga aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg
     6301 acttaaatta aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg
     6361 cattcgcact aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat
     6421 tatctttccc ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca
     6481 atgtttcccc cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga
     6541 ttgagcagaa aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag
     6601 agtgatacag aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata
     6661 gtgatgatgg cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg
     6721 aaaggcctcg aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt
     6781 ccccatggct ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg
     6841 gccgccatgt ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg
     6901 cgtaaattcg gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca
     6961 gtacgccagg gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt
     7021 gaatttgctg atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg
     7081 aacatcagtc aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc
     7141 ctgactattg aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg
     7201 attgcccgac aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat
     7261 atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga
     7321 aattataaag tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat
     7381 cccccttccg tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt
     7441 taatttatg
//
LOCUS       DAJTRN010000156         7094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRN010000156 DAJTRN010000000
VERSION     DAJTRN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            554..1204
                     /gene="csgD"
                     /locus_tag="OO328_003721"
     CDS             554..1204
                     /gene="csgD"
                     /locus_tag="OO328_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5932128.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1209..1598
                     /gene="csgE"
                     /locus_tag="OO328_003722"
     CDS             1209..1598
                     /gene="csgE"
                     /locus_tag="OO328_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5932129.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1623..2039
                     /gene="csgF"
                     /locus_tag="OO328_003723"
     CDS             1623..2039
                     /gene="csgF"
                     /locus_tag="OO328_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5932130.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2066..2772
                     /locus_tag="OO328_003724"
                     /pseudo
     CDS             2066..2772
                     /locus_tag="OO328_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(2905..3396)
                     /locus_tag="OO328_003725"
     CDS             complement(2905..3396)
                     /locus_tag="OO328_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5932131.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3498..4052)
                     /gene="ycdY"
                     /locus_tag="OO328_003726"
     CDS             complement(3498..4052)
                     /gene="ycdY"
                     /locus_tag="OO328_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5932132.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4076..4813)
                     /gene="ycdX"
                     /locus_tag="OO328_003727"
     CDS             complement(4076..4813)
                     /gene="ycdX"
                     /locus_tag="OO328_003727"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5932133.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4868..5806)
                     /gene="ghrA"
                     /locus_tag="OO328_003728"
                     /pseudo
     CDS             complement(4868..5806)
                     /gene="ghrA"
                     /locus_tag="OO328_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6041..6128
                     /locus_tag="OO328_003729"
     tRNA            6041..6128
                     /locus_tag="OO328_003729"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6075..6077,aa:Ser,seq:gga)
     gene            complement(6278..6559)
                     /locus_tag="OO328_003730"
                     /pseudo
     CDS             complement(6278..6559)
                     /locus_tag="OO328_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6841..>7094)
                     /locus_tag="OO328_003731"
     CDS             complement(6841..>7094)
                     /locus_tag="OO328_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932134.1"
                     /translation="QYSTGGKSTLLGISKRGDKNLRRLLVQCARSFIMRLERKHGKLA
                     EWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA"
BASE COUNT         1930 a         1703 c         1566 g         1895 t
ORIGIN      
        1 tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt ccgctatcaa aaatcaccag
       61 acagtcattc ttcttgcccg tcgctgattg ctgcgatatg tcttgcgcac aagccgtgac
      121 aaacctcgtc tttttgcagt aaaaaaattg tccacggagg tgtggagaaa aaacaaaaac
      181 gttttacatg acgaaaggac tacaccgaaa tattttttat atgtattatt agtaagttag
      241 caccatttgt atgatttttt aaaattgtgc aataaaaacc aaatgtacaa cttttctatc
      301 atttctaaac ttaataaaac cttaatgtta acattttaat ataatgagtt acattttgtt
      361 acatgtttaa cacttgattt aagatttgta atggctagat tgaaatcaga tgtaatccat
      421 tagttttata ttttacccat ttagggctga tttattacta cacacagcag tgcaacatct
      481 gtcagtactt ctggtgcttc tattttagag gcagctgtca ggtgtgcgat caataaaaaa
      541 gtggggtttc atcatgttta atgaagtcca tagtattcat ggtcatacat tattgttgat
      601 cactaaacct tctttgcagg cgacagctct cttgcagcac cttaaacaat cgctagcaat
      661 tacaggaaaa ttacataata ttcaacgttc tctggacgat atctcttcag gctctattat
      721 tcttctggat atgatggaag cggataaaaa gcttatccat tattggcagg atactttgag
      781 caggaaaaac aacaatatca aaatattgtt gctaaatacg cctgaagatt acccgtaccg
      841 cgacattgaa aactggcctc atatcaacgg cgttttttat gccatggagg atcaagaacg
      901 tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc tactttacgc aaaagcttgc
      961 cagttacctg attacacatt ctggtaacta tcgttataac agcacggaat cagccctcct
     1021 tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc ggcgcgtcta ataacgagat
     1081 cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg catctttata atcttttcaa
     1141 gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg gcaaacgata acctcaggcg
     1201 ataaagccat gaaacgttat ttacgctgga ttgtggcggc agaatttctg ttcgccgcag
     1261 ggaatcttca cgccgttgag gtagaagtcc cgggattgtt aactgaccat actgtttcat
     1321 ctattggcca tgatttttac cgagccttta gtgataaatg ggaaagtgac tatacgggta
     1381 acttaacgat taatgaaagg cccagtgcac gatggggaag ctggatcaca ataacggtca
     1441 atcaggatgt tattttccag acttttttat tcccgttgaa aagagactcc gagaaaactg
     1501 tcgtctttgc actgattcaa actgaagaag cactaaatcg tcgccagata aatcaggcgt
     1561 tattaagtac ggacgatttg gcgcatgatg aattctaaat aaaaaattgt tcggaggctg
     1621 caatgcgtgt caaacatgca gtagttctac tcatgcttat ttcgccatta agttgggctg
     1681 gaaccatgac tttccagttc cgtaatccaa actttggtgg taacccaaat aatggcgctt
     1741 ttttattaaa tagcgctcag gcccaaaact cttataaaga tccgagctat aacgatgact
     1801 ttggtattga aacaccctca gcgttagata actttactca ggccatccag tcacaaattt
     1861 taggtgggct actgtcgaat attaataccg gtaaaccggg ccgcatggtg accaacgatt
     1921 atattgttga tattgctaac cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa
     1981 ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa taactcaacc gatttttaag
     2041 ccccagcttc ataaggaaaa taatcatgca gcgcttattt cttttggttg ccgtcatgtt
     2101 actgagcgga tgcttaaccg ccccgcctaa agaagccgcc agaccgacat taatgcctcg
     2161 tgctcagagc tacaaagatt tgacccatct gccagcgccg acgggtaaga tctttgtttc
     2221 ggtatacaac attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc
     2281 cactgctgtt ccgcaaagcg ccagggctta caaaacctgc ttaacgagcg caaggttatt
     2341 cgtgcggcac aagaaaacgg cacggttgcc attaataacc gaatcccgct gcaatcttta
     2401 acggcggcaa atattatggt tgaaggttcg attatcggtt atgaaagcaa cgttaaatcg
     2461 ggcggtgttg gggcaagata ttttgggatc ggtgcagaca cgcaatacca gctcgatcag
     2521 attgccgtga acctgcgtgt cgtcaatgtg agtaccggtg agatcctctc ctcggtgaac
     2581 accagtaaga cgatactttc ctatgaagtt caggccgggg ttttccgctt tattgactac
     2641 cagcgcttgc tggaagggga agtgggttac acctcaaacg aacccgttat gctgtgtctg
     2701 atgtcggcta tcgaaacagg ggtcattttc ctgattaatg atggtatcga ccgtggtctg
     2761 tgggatttgt aaaataaagc agaacggcag aatgacattc tggtgaaata ccgccatatg
     2821 tcggttccac cggaatcctg acagataaaa atgccggaat taataatccg gcatttactg
     2881 tttaagcgaa agcgtgagtg cccctcacgc tttatttttg atttcctgct cacggtgagc
     2941 cgtctttgca ctacgcgccg ccagccacag gccgctgttt ttcattgcgt aaccaaatac
     3001 cagcccaagc gccagcgaag gtaacaccag tttccagtca ccctgcccgg caaacgtcgc
     3061 gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc agctgtttgg cctgaataca
     3121 catcagaaac gcgacaatac cggttatgac ataaccgaga atttccagat gtggtgccag
     3181 tgcactaccg taaataatga ccatcgccca caccacgcca ctaagcaggg ttgcagcgga
     3241 gatcgccagc cctttcagcc caccttgcgg gcaagcaaag taggccgtac agcccaggaa
     3301 gcccgcccag cttagtaagc ccagggaaac agccacccat ccccagatac cggagagaat
     3361 gcctgttgtg attgcaatag agagaagtat gttcatgccg cgcatgatag cagaaaacgc
     3421 ggccatgaat gagatcgagc acacatttta tgcaacaaca aattatctgt tgcagttatg
     3481 agtgacgatg atcacattta ctcttcagaa tcttcttcca gctcgtccca cattgcacta
     3541 atggcatcac gggttagcgg tgccatggtg cgccagaaag gcgtggttgc atgggcctcc
     3601 actttgccga ggaacgcacc acaccagggt aacagatact cactgaacag tgtttccagt
     3661 gcttcgctct catcttccgt tgactgatct tccagccagg aagccgcaag cagcaatgtg
     3721 ccgatgtgat cggctggcgt atccgccagt agcatccctc gttcggaaag aaaagcgcgc
     3781 acttccgctt ccgttgcgtc ctcaacccat gcgctacgat atggcggcac ggcacattca
     3841 tcgccgataa acaacgcatt gtaatcggca gagacttgcg tcatatcaca gcttttctgt
     3901 aaacgcgtca gtaactcatc ctgctccagt ggccagttcg cagccagttt cccctcacga
     3961 atcagagtaa acagcggcac cagtaaagga tcttgcggtt ggcggtaata gagcgaaccc
     4021 agcacacgac agaggataga aaactcgttc attaatatat ttccattact agtaattaaa
     4081 ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc aaggaagttc agtaagcggc
     4141 gcggagaaac attcaaaatg cgctctagcg gaaaatctac cgcgtcgagg attttaagac
     4201 actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc cgagcctaat gccacccaac
     4261 ctccagcatc acgtaccgct gcagctaccg cacgacagtt gtcttcactg cccttacgtg
     4321 agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg atgtttcgcg gctgcttcag
     4381 caacggcttt aacatctatt tcatatttcg gatttccggg atggcttatt atatgcacat
     4441 tgccgctggc gatagtggag atcatcgctt gtgtattggt cgctttgtca tgtggcgcaa
     4501 aaaccggctc atgaaaaccg gcaataatca gatccagcga gtcaaacatt ttaccgctgc
     4561 agtcaatttc accatcaaca tttttaatgt tagcttcaat gccgcgcagg atccctaccc
     4621 catcaaccac tcgcggccag atacgcatgt taatgaagtg ccagtgatgc ggcgcatctt
     4681 ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat gcccttttgt ttggcctggg
     4741 caatgtaatc actcaatgtg ctatatgcat gtgtgctggc aacggtatgc atatgaaggt
     4801 cgacgggata cataactctc tcctgtattc aattcccgcc aaggatagca ggaatcctga
     4861 tgctttatta gtagccacgt gcgcggtcga cttgcccgca gaccctctcc cctttttcga
     4921 gctgggcaat agtgcgagaa atgtactcca cagcttcagc gggacgggta atcgcggcga
     4981 catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg actttcaggc ggtaagggtt
     5041 cacgattaaa aacatccagc attgcgcctt taactttccc gctatccagc gccgcgagca
     5101 ggtcatcttc cacaacatga acaccacgcg ccaggttgag gagatacgcg ccatcaggta
     5161 atttttcgag taattgttga ttaataatgc cgacggtttc aggggtattc ggtaacaaat
     5221 taatcaatac ccgacattgg ctcagaaatg cagacagttc ttcccgtccg gcaaagcttt
     5281 gcacgccagg ccacgattta cgggttcgac tccagcaacg cagcggaaag cgctaggttt
     5341 gcagactctg agcaacttta cagcccagta cgcctgcgcc caaaatgccg atggtaaaat
     5401 cttcccgatg atattcaggc agcggttgcc aatgcgaact attttgctgg atgcgataat
     5461 cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc atattcctgc atttgctcgc
     5521 ccataccggt atcttccagg cgaaaaagtg gaacagaagg gttcagcatt tcagggtgtg
     5581 cctgtagctt gctcaaaata gaatcaacac cggccccgag tgcgaacacc gctttaagat
     5641 cgcgccctgc cagcatttca acaggaggat gccagactaa aacataatca gcagagtcat
     5701 tatctccgct tttccatgcc ctgactcttg cctgaggaat agctttgcgc agtgcctcaa
     5761 tccaccattg ggtatcgaac gttgggtgat aaaagatgat atccatactg actcccgaaa
     5821 agcgttgtgc gaaatttatt cgcacttatc gttatgatct acaaaggcca ccagcataac
     5881 aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat taaaaccaca cattgattga
     5941 atgtttgaat aaacgcgcga ttttttcaaa aagtttgttg acctcaggtc atgatttccc
     6001 taaattagcg cccgttccag caagacagga acgacaattt ggtgaggtgt ccgagtggct
     6061 gaaggagcac gcctggaaag tgtgtatacg gcaacgtatc gggggttcga atccccccct
     6121 caccgccaga ttataagtgc atgttattgt ttaacatgcg cttttttatt tttccttcca
     6181 gacagtatgc catcgggtat gccatctgaa accggtttat atgccccgcg atcatatctt
     6241 atcggacaat ttccgatctt tgagcatact ggcaaagcta tactgccagc atatcaggat
     6301 agtgttttgc gataatatca ttcagtctgt caaccagttc tggttttctg aacgtgatat
     6361 gcgcggagcc cttcttaaag tatctgatgc tgaacatctc atcttcatag ctctctttac
     6421 cctgaacaga ctgaatgtga tcatccagac gaagggtgat gtttccccgg ttgtcgggga
     6481 ttggtttacc gctgaacagg tgcaacattc tttccagatc ggcgagtcgg tcagcctgtt
     6541 gtccggtgat aagatgaaat ctatggtcac ccccgttttt gcaacactga tttttgatta
     6601 atggttggct tgcttaaatc tatccggcgt ctctgtgggc aaggatgccc gcgccccgat
     6661 gagttccgca cctgataatc ctaaaaatcc cctcggcttc agtgagccat ttcatctgtt
     6721 aggtttttaa tcaggtcgat ttgccgttca tgtcatcaat aatcagtctt cgcaaaacca
     6781 gaaaggtaac ttgtatctta ttgtttaaaa taaactaatt gagttatatc agcatctata
     6841 tcaggcctga tattcttttt tctgagtagt gattgcccag gcaattcgag ccagtttatt
     6901 tgccagagcg caggtaacca cattcgaatg cttcttgttg agttgctccc gaacccactc
     6961 agcaagtttt ccatgcttgc gttcaagtcg cattatgaat gagcgggcac actgaaccag
     7021 taaccggcgt aaatttttat cacctcgctt actgataccc aacagagtcg attttcctcc
     7081 agtgctatac tgcc
//
LOCUS       DAJTRN010000157         7006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRN010000157 DAJTRN010000000
VERSION     DAJTRN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(176..1078)
                     /locus_tag="OO328_003732"
     CDS             complement(176..1078)
                     /locus_tag="OO328_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5932135.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1157..2110)
                     /gene="lsrR"
                     /locus_tag="OO328_003733"
     CDS             complement(1157..2110)
                     /gene="lsrR"
                     /locus_tag="OO328_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5932136.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2359..3894
                     /gene="lsrA"
                     /locus_tag="OO328_003734"
     CDS             2359..3894
                     /gene="lsrA"
                     /locus_tag="OO328_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5932137.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3888..4916
                     /gene="lsrC"
                     /locus_tag="OO328_003735"
     CDS             3888..4916
                     /gene="lsrC"
                     /locus_tag="OO328_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5932138.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4916..5908
                     /gene="lsrD"
                     /locus_tag="OO328_003736"
     CDS             4916..5908
                     /gene="lsrD"
                     /locus_tag="OO328_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5932139.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5920..6943
                     /gene="lsrB"
                     /locus_tag="OO328_003737"
                     /pseudo
     CDS             5920..6943
                     /gene="lsrB"
                     /locus_tag="OO328_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1629 a         1768 c         1834 g         1775 t
ORIGIN      
        1 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
       61 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      121 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      181 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      241 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      301 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      361 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      421 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      481 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      541 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      601 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      661 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      721 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      781 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      841 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
      901 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
      961 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1021 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1081 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1141 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1201 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1261 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1321 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1381 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1441 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1501 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1561 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1621 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1681 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1741 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1801 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1861 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     1921 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     1981 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2041 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2101 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2161 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2221 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2281 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2341 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2401 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2461 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2521 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2581 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2641 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2701 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2761 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2821 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     2881 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     2941 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3001 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3061 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3121 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3181 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3241 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3301 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3361 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3421 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3481 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3541 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3601 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3661 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3721 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3781 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3841 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     3901 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     3961 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4021 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4081 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4141 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4201 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4261 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4321 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4381 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4441 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4501 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4561 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4621 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4681 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4741 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4801 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4861 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     4921 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     4981 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5041 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5101 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5161 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5221 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5281 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5341 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5401 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5461 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5521 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5581 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5641 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5701 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5761 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5821 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     5881 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     5941 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6001 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6061 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6121 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6181 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6241 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6301 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6361 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6421 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6481 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6541 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6601 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6661 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6721 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6781 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6841 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     6901 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     6961 gagtaagtta tggcagattt agacgatatt aaagatggta aagatt
//
LOCUS       DAJTRN010000158         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRN010000158 DAJTRN010000000
VERSION     DAJTRN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            240..2672
                     /locus_tag="OO328_003738"
     CDS             240..2672
                     /locus_tag="OO328_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5932140.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO328_003739"
     CDS             2739..4208
                     /locus_tag="OO328_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5932141.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO328_003740"
     CDS             4208..5869
                     /locus_tag="OO328_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5932142.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO328_003741"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO328_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5932143.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO328_003742"
     CDS             complement(6478..>6795)
                     /locus_tag="OO328_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932144.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTRN010000159         6749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTRN010000159 DAJTRN010000000
VERSION     DAJTRN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            40..1464
                     /gene="sbcB"
                     /locus_tag="OO328_003743"
     CDS             40..1464
                     /gene="sbcB"
                     /locus_tag="OO328_003743"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5932145.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1507..1734)
                     /locus_tag="OO328_003744"
     CDS             complement(1507..1734)
                     /locus_tag="OO328_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5932146.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1748..2806)
                     /gene="yeeE"
                     /locus_tag="OO328_003745"
     CDS             complement(1748..2806)
                     /gene="yeeE"
                     /locus_tag="OO328_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5932147.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2985..4343)
                     /gene="plaP"
                     /locus_tag="OO328_003746"
     CDS             complement(2985..4343)
                     /gene="plaP"
                     /locus_tag="OO328_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5932148.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4333..4395)
                     /gene="yoeI"
                     /locus_tag="OO328_003747"
     CDS             complement(4333..4395)
                     /gene="yoeI"
                     /locus_tag="OO328_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5932149.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4556..5539)
                     /locus_tag="OO328_003748"
     CDS             complement(4556..5539)
                     /locus_tag="OO328_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5932150.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5585..6409)
                     /locus_tag="OO328_003749"
     CDS             complement(5585..6409)
                     /locus_tag="OO328_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5932151.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6492..6746)
                     /gene="yoeB"
                     /locus_tag="OO328_003750"
     CDS             complement(6492..6746)
                     /gene="yoeB"
                     /locus_tag="OO328_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5932152.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1824 a         1775 c         1581 g         1569 t
ORIGIN      
        1 aacagacagt taattaacta cagtggacaa tgctagacaa tgacagacac agataaacaa
       61 cctaccttcc tctttcacga ttacgaaacc tttggcacgc accccgcgtt agatcgccct
      121 gcacagttcg cagccattcg caccgatgac gaattcaatg tcatcggcga acccgaagtc
      181 ttttactgca agcccgcgga tgactattta ccccggcctg gagcagtatt aattaccggt
      241 attaccccgc aggaggcacg ggcgaaagga gaaaacgaag ccgcgtttgc cgcccgtatt
      301 cactcgcttt ttaccgtacc gaagacctgt attctgggct acaacaatgt gcgtttcgac
      361 gacgaagtca cacgcaacgt tttttatcgt aatttctacg atccttacgc ctggagctgg
      421 cagcatgata actcgcgctg ggatttactg gatgttatgc gtgcctgtta tgccctgcgc
      481 ccggaaggaa taaactggcc tgaaaatgat gacggtctac cgagctttcg ccttgagcat
      541 ttaaccaaag cgaatggtat tgaacatagc aacgcccacg atgcgatggc tgatgtgtac
      601 gccactattg cgatggcgaa actggtaaaa acgcgtcagc cacgcctgtt tgattatctc
      661 tttacccatc gtaataaaca caaactgatg gcgttgattg atgttccgca gatgaaaccc
      721 ctggctcacg tttccggaat gtttggggca tggcgtggca ataccagctg ggtggcaccg
      781 ctggcgtggc atcctgaaaa tcgcaatgcc gtaattatgg cggatttggc aggagacatt
      841 tcgccattac tggagctgga tatcgacaca ttgcgcgagc gtttatatac cgcaaaagcc
      901 gatcttggcg ataacgccgc cgttccggtt aagctggtgc atatcaataa atgtccggta
      961 ctggcccagg cgaatacgct acgcccggaa gatgccgacc gactgggaat taatcgtcag
     1021 cattgcctcg ataacctaaa aattctgcgt gaaaatccgc aagtgcgtga aaaagtggta
     1081 gcgatattcg cggaagccga accgtttaca ccttcagata atgtggatgc acagctttat
     1141 aatggctttt tcagtgacgc cgatcgtgca gcaatgaaaa ttgtgctgga aaccgagccg
     1201 cgtaatttac cggcactgga tatcactttt gttgataaac gaattgaaaa gttgttgttc
     1261 aattatcggg cacgcaactt cccggggacg ctggattatg ccgagcagca acgctggctg
     1321 gagcaccgcc accaggtctt cacgccagag tttttgcagg gttatgctga tgaattgcag
     1381 atgctggtac aacaatatgc cgatgacaaa gagaaagtgg cgctgttaaa agcactttgg
     1441 cagtacgcgg aagagatcgt ctaatttgaa gccgccgccg ctggtacggc ggaggacaat
     1501 gttgagtcag gctttttgaa cggtgatgct ccacgctgca tcgccaatct gctgataatc
     1561 ggtgatggca tgtccttctt ctgccgccca ctgtgggatt gcttcagttg cctgggtgca
     1621 atcaaactca attaccaact catcgccgct ggccatttct gccagtgctg cttttgcttc
     1681 tatgagtgga aacggacaga cctgcgtcac cacatccagc tttttaatcg ccatctttac
     1741 accttactta atttgccgca gcagttgcca gtcgcgcctt acgctgcggt cgaacataca
     1801 caagccagga cgcagtccag actccgagaa tcataaatac aaggccaatc cagccctgcc
     1861 aggtcatcat tgcagtcata accagtccat taccgataga gcaaccacct gcaatactgg
     1921 caccgaagcc catcagtaca ccacctaacc cgctacgtaa tgttgtttgt gcatcagctg
     1981 cgcgaacgcg gaactcacgg ctcgctttgg ctgcaataaa tgcctccacg aagatcccta
     2041 acaccaggaa aacgccccag ttaatgtatt tcacgtcacc cgcgaccaga aattgaagaa
     2101 tattggccgt tggggaagtg actcctaacc caaacatgcg tccggttgct tcactcaggg
     2161 gccaggctaa aagcgcaatc aaaccgatga gtacagcagt gacaaaggga tgccagcgtt
     2221 tttcaaacag aatatgagcg atcccggttc ggcgcggcgg taaggtcgcg actttgagtt
     2281 ttggcttctt caactctttc atcaccaccc agagcgttat caccagcaaa acggtaacca
     2341 acggccacac agacaaatta aaagtctcag caatagagtt atgttcagta gtgtagtgct
     2401 gcaaggtttg atttaaacca ctggcgtgtg gagaacgcat caccgcactc atcaccatat
     2461 aagtgaaaag cgcgatccaa ctgccgatca atccctcacc cgcgcgatac caggtcccgg
     2521 tggcacatcc acccgccaga acaattccca gcccgaaaag atacccacct ataacagtac
     2581 caagccacgg gaacgccccg gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa
     2641 cccccacgct ttgtgccgaa attgcaatca gcaaggcgta aaacatgcga ttatttttcg
     2701 cgatatacat atcgcgaaaa ccaccagtca ggcaaaaacg cccacgctgc atgacaaatc
     2761 ccagcagagc accacaaatt agcccgctta atatcattga aaacataaat taattaacca
     2821 gatgaatgtt aatggggaaa tttttcatga ctggtggaat gcagaccaat aaccaaattc
     2881 tctaaattag aacaaatggt tattaatgag gatttcgata aaaaagaata aaaaaaccgg
     2941 agccaaatgt tcattcgact ccggtttatt attagaaaag atgattatgc tacgtcttcg
     3001 tactgcggaa ccggattacg gaagctcttg gtgacgcaag ccaggtaaat cagaccgata
     3061 gcagcccaga tcagacccag aaccattgag ctttcttcca ggttaaccca cagcgcacca
     3121 acggtcagcg caccacacat cggcaggaac agatactgga agtgatcttt cagcgtcttg
     3181 ttacgcttct cacggatcca gaactgcgag atgaccgaca ggttaacaaa ggtgaacgcc
     3241 accagcgcac caaagttaat cagcgccgtt gccattacca ggtcgaagtt gattgccagc
     3301 agggcaatcg cgccaaccag gatgatgttc atcgccggag tacgccattt cgggtgtaca
     3361 taaccgaaga agcttttcgg gaacacgccg tcacgaccca ttacgtacat cagacgcgct
     3421 acgcctgcgt gcgccgccat accggacgcc agtacggtaa tggtggagaa gatcagcgcg
     3481 ccaacctgga acgctttacc cgcaacgtac agcatgattt caggctgtga cgcatccgga
     3541 tctttaaagc gagagatatc cgggaagtac agctgcagga agtaagttgc aaagatgaag
     3601 atcatgccgc caatcagcgc ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct
     3661 ttggtttctt ccgacagatt gctgatgccg tcaaagccgg taaaggagaa gcacaggatt
     3721 gtcgccccgg taatcatcgg gataacatgc gcatcgccag accagaacgg acgcgtgctc
     3781 gccagcgtac cagcgccttc accttcaaat acgccataaa caaccatgcc cagaatcacc
     3841 gcgatcagca ctacctgcaa cacgacgatt acagtgttga cgttcgctac ggatttcaga
     3901 ctacgcaggt taaaggcggt cataaaggcc accagcgcca ccacaaacat ccacgatgga
     3961 atggaaggca ccagagcttc aaaatagatt ttcgccagca gaatgttgat catcggcgcg
     4021 aacagatagt cgagcagaga agaccaaccc accataaagc cgacagtcgg gctaatggat
     4081 ttctgggcgt aagtgtatgc agagccagca gaaggatagc ggcgaaccag cttcccgtag
     4141 ctcagagccg taaacaggat cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca
     4201 tccgtgaggc ctgaaacgat accaaatgta tcaaacagcg tcatcggctg catataggct
     4261 agacccatca ttacaaccgg aactaacgta agcgttttac gcaattccac gcgagaggtg
     4321 tttggagtaa cgttatgcga catggttatt ctcctttacg gtgataaccg tcgcgtaagc
     4381 aaaaaattgc cccatttttt tggattcctc agcgacaaca actgtcgatt tttagtaaat
     4441 atctatccgg tacgaagccc ggcctcttgg tatgaatgat tggtttgaag caaaaaataa
     4501 ccgacgctaa ttaaacgtcg gtttttaatc attttttgac agcggcgcat tgtgcctaaa
     4561 tgggggggaa atgacaagag aatgagaggc ttgtcataat aatttttctt tgaatggctg
     4621 attttccgtc atcagatttt gcataaacac cggcgatcgc actatttgct aaaatttcat
     4681 ctcgccacca gccgctggca agccctgcgg tctgttcatt ccagccaatc cagacaggtt
     4741 caaagagagt ttgggcgacc actttcttct cgattaacgc gccactatcg agaaaacgtt
     4801 gcgccagata ccgtggtaaa tagccgcagc caaggccgct aatttgcagc tccagtttgg
     4861 ttttaaaatc gaaaacggta atggcttctt gttcgtcgag aagttgtgag gccgtagaag
     4921 cgcctgcctg cgcagtatcc cccaccacaa tcgcccgata tcgtttaatg atacgacggt
     4981 ttaacggttc ttcttcctgg gctaacggat gatgaggcgc gaccgcaaag acttgttcaa
     5041 gatcgcccaa ccgcgagaaa ccaaactcac tggaagatgg tggctcatgc atcgcgccaa
     5101 cgataatatc cgctcgcccc tgagtcaggg catcccagga accaccgagt acgccattga
     5161 taaacttcag gcgcgtcacg ctatgatgtt gataaaaagc ttcaatgaga ggcgcaagca
     5221 gagaaaaagg aaacgtgtcg tccacgccaa tcaccagctc gttttcccag ccttcatgca
     5281 gtttgatcgc ctgcttttcc agttctcgca cggtatgcag aacttcccgc cctttctcta
     5341 ataacatttt tccggtgcgg gtgaatttag cgcggtgtcc gctacgatca agcaattgaa
     5401 tatttaggtc gctttccagc ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga
     5461 gtttggctga cgccgccgca aaactgccct ctttttctat ggcatcgagg atcatcaaca
     5521 catccagcag tggtttcata cacgccccct tgcagtgcat gcggcgtcct gctggcgtga
     5581 tcgtttactc caacggcatt accagcggat cgggatactg gtattcaaat cccagttcat
     5641 tacaaatccg actgccatca ataatcttac ctttgccgct gtccagacta tttctgaact
     5701 gcggaggttg cagccccagt aaacgggcca tctgcggata gaaaacatta cgcgcagggt
     5761 gagctggcgc acatatatta tagatgtgtc cgcctttagg tgcctgtaac aacagagtga
     5821 tagcgccaat aacatcttct aaatggacta aattaacacc atgttcacca tcaggggcgg
     5881 tttttccggc aaagaagcgt cccggatgac gtcccggtcc gaccaggccc gcaagacgca
     5941 gaatatcgac cgaagtaccg ggtaaattgt gcagccagtc ttcgagttct tctaatactc
     6001 gtccactgtt ggttaccgga ttacgcgggg tagtttcttt aaccgtgcct tgcgcgtcgc
     6061 catagacaga tgttgagctg gtaaaaataa ttcggggaat acgatgggcc agcgcgctat
     6121 ccactaactc ttgtaccgct tgtaaataga actcatcgcc ggggccgcta cgacgtgccg
     6181 gaagcgtaat gaccagcgca tcggcatcca taagggcatc cagatcgtca gaatcgcaaa
     6241 ctaactcagg ctccatgcga agcagatagc tatcaatgcc actcattcgg gccgcttcga
     6301 caccatcttg tgtggtttta ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg
     6361 ccagcggcat acctaaccac cctaacccga caattgcgac ctttttcatc cgttatctcc
     6421 tgatacacct tagatctata aggctacgct agcgtatcaa aactgacaat tcattctatg
     6481 aatgaatctg ttcaataatg ataacgacat gctgcaatga gcagtgaatc atcagtaacc
     6541 gcgtatacca gacggtgctc ctctgtaatg cgtcgggacc agaaacctga caaattatgt
     6601 ttcaggggtt ctggtttccc cttaccttca aatggtgttc tgcgggtatc tttgataagt
     6661 tcattgatct ttttaacaat tcgcttatct gtttcctgcc agtacagata atcatcccat
     6721 gattcctcag accagattag tttcactca
//
LOCUS       DAJTRN010000160         6542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRN010000160 DAJTRN010000000
VERSION     DAJTRN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            461..1849
                     /gene="narU"
                     /locus_tag="OO328_003751"
     CDS             461..1849
                     /gene="narU"
                     /locus_tag="OO328_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5932153.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1931..5671
                     /gene="narZ"
                     /locus_tag="OO328_003752"
                     /pseudo
     CDS             1931..5671
                     /gene="narZ"
                     /locus_tag="OO328_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5668..>6542
                     /gene="narH"
                     /locus_tag="OO328_003753"
     CDS             5668..>6542
                     /gene="narH"
                     /locus_tag="OO328_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5932154.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1564 a         1717 c         1695 g         1566 t
ORIGIN      
        1 taagttggag tcattacctg accttagatg aaataaacaa tacttactat aataacgatt
       61 attacaatca ggcaataaga gaggaaccga tagactttct ggacagatcg ttttcctcca
      121 attcatggcc tttttagtga ataaatcatc actaattcta taagtgtctg gtcttgttga
      181 gaatatagtt aatatgtaac cctttagatt cacgatctga agggttgaaa catgacatac
      241 gttcaaagat taacccccgt ttgaaggtta gcttgttagt tacatttagt aacacatatc
      301 tggctactta atccccattc tgcccccgcg gttacgcttt cttatctatt ctttcaatca
      361 gcacttcacc caattcatcc ttaattctaa taactggcta ttgcgcctta cctcctgttt
      421 tgtctttttt cattaccaat gtgtgcatat gaggaacaat atggcactgc aaaatgagaa
      481 aaatagtcgt tatcttttac gcgactggaa accagaaaat ccggccttct gggaaaataa
      541 aggaaaacat attgctcgaa gaaacctctg gatatcagtc agttgtctac ttcttgcctt
      601 ctgtgtctgg atgctattta gcgcagttac cgttaatctc aataaaatcg gttttaattt
      661 tactaccgat caactctttt tattaaccgc attaccctcc gtttctggcg cattattgcg
      721 tgttccctac tcctttatgg tgcctatatt cggtggacgc cgatggccgg tttttagtac
      781 tgcaatcctg attattcctt gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc
      841 ttttgggata tttatcgtta tcgctttgct atgcggtttt gcaggtgcaa actttgcttc
      901 gagcatgggc aatatcagtt tcttctttcc aaaagccaaa caagggagcg ctcttgggat
      961 taatggcgga ttaggaaact taggtgtaag tgtaatgcag ctggttgcac cgctggtcat
     1021 ttttgtacct gtatttgcct ttctcggcgt caatggcgta ccgcaggccg acggttcggt
     1081 gatgtcgctg gcgaatgccg catggatttg ggtaccgcta ctggcgattg ccacgatcgc
     1141 cgcctggtca gggatgaatg atatcgccag ttcacgcgcc tcaattgccg accagctccc
     1201 tgtcttacaa cgcctgcatc tctggctgct gagcctgctt taccttgcca ccttcggttc
     1261 gtttatcggt ttttctgcgg gttttgccat gctggcaaaa acccagttcc cggatgtgaa
     1321 tattctgcgc ctggcgttct ttggcccatt tattggtgcc atcgcgcggt cggttggtgg
     1381 tgctatttcc gataaattcg gcggcgtgcg ggtgacgttg atcaacttta tttttatggc
     1441 gattttcagt gccctgctgt tccttacctt accgggcaca ggctccggta atttcatcgc
     1501 cttttacgcc gtatttatgg ggctgtttct gaccgcgggt ctgggaagtg gttctacttt
     1561 ccagatgatc gccgtcatct ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg
     1621 tagtgatgag caagctcaga gagaagccgt caccgaaacg gcggcaacgc tgggctttat
     1681 ctcagccatt ggcgcagtgg gcggcttttt tattccgcag gcatttggca tgtcgctcaa
     1741 tatgaccggc tctccggtcg gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt
     1801 gctgctgacc tggctggttt atggtcggcg gaagttcagc caaaaataag agatcgatgc
     1861 ctgatgtaca accatgtcgc atccgacaag gcatcagatc ccaattcatt cctggagcag
     1921 gagtcatgtc atgagtaaac ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac
     1981 ctttgccgat ggtcacggac aggtgatgca tagcaaccgc gactgggagg acagctatcg
     2041 ccagcgttgg cagttcgaca aaatcgtgcg ttccacccac ggcgttaact gtacaggctc
     2101 ctgtagctgg aaaatctacg ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga
     2161 ctacccgcgc actcgccctg acctgcccaa tcatgaacct cgcggctgcc cgcgtggcgc
     2221 aagttactcc tggtatcttt acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg
     2281 actgattgaa ctgtggcgcg aagccctcaa acaacacagc gatccggtac tggcgtgggc
     2341 atcgattatg aatgatccgc aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg
     2401 gtttatccgc tccaactggc aggaactaaa ccagctgatt gccgccgcta acgtctggac
     2461 catcaaaacc tacggcccgg atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat
     2521 ggtttcttac gccgccggaa cgcgttatct gtcgctgctt ggcggcacct gttaaagttt
     2581 ctacgactgg tattgcgacc tgccgcccgc ctcgccgatg acctggggcg agcaaaccga
     2641 cgtaccggaa tctgccgact ggtataactc cagctacatc atcgcttggg ggtctaacgt
     2701 accgcagaca cgtacgccgg acgcccactt ctttaccgaa gtacgctaca aaggcactaa
     2761 aaccatcgcc attacccctg actactctga agtggccaaa ttgtgcgacc agtggctggc
     2821 accgaaacaa ggcactgata gcgccctggc gatggcaatg gggcatgtga ttttaaaaga
     2881 gtttcatctc gataatccca gcgactactt tatcaactac tgccgccgct acagcgacat
     2941 gccgatgctg gtaatgctgg agcctcgcga cgatggtagc tacgttcccg ggcgcatgat
     3001 ccgcgcatct gacctggtgg atggactggg cgaaagcaac aatccgcagt ggaaaaccgt
     3061 agcagttaat accgcaggtg aattggtagt gccgaacggt tcgattggtt tccgctgggg
     3121 agaaaaaggc aaatggaatc tggaatccat tgccgccggt acggaaaccg aattgtcgtt
     3181 aaccctgctc ggtcaacatg acgctgttgc aggcgtggcc ttcccctact ttggcggcat
     3241 tgaaaatccg cattttcgca gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt
     3301 taaaaacctg acgttagtcg atggcaacac ctgtccggtg gtcagcgttt atgatttggt
     3361 actggcgaat tacggcctcg atcgcgggct ggaagatgaa aacagcgcga aagattacgc
     3421 tgaaatcaaa ccgtacaccc cagcctgggg tgagcaaatt accggcgtac cgcgccagta
     3481 tattgaaacc atcgcccgcg aatttgccga taccgcccat aaaacgcatg ggcgctcaat
     3541 gattatcctc ggcgcaggtg ttaaccactg gtatcacatg gacatgaact accgcgggat
     3601 gatcaatatg ctgatcttct gcggttgtgt cgggcaaagc ggtggcggct gggcacacta
     3661 tgtcggccag gaaaaactgc gcccacaaac cggctggttg ccactggcct ttgcgctcga
     3721 ctggaaccga ccaccgcgcc agatgaacag cacctcgttt ttctacaatc attccagcca
     3781 atggcgctat gaaaaagtct ctgctcagga gttactttca ccgctcgccg atgccagtaa
     3841 gtacagcggt catctgattg atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc
     3901 tgcgccgcag ctggggcgta acccgctcgg gattaaagct gaagccgaca aggccggatt
     3961 atcccccaca gaatttaccg cccaggcgct gaaatcgggc gatttacgta tggcctgcga
     4021 acaaccagat agcggcagca atcatccgcg taatttgttt gtctggcgct ctaacctgct
     4081 tgactcctcc ggcaaaggcc acgagtatat acagaagtat ctgctgggga ccgaaagcgg
     4141 gattcagggc gaggaactcg gtgccagcga cgggatcaaa ccggaagaag tcgagtggca
     4201 aactgcggcg attgaaggca agctcgacct gctggtgacg ctcgacttcc gcatgtccag
     4261 tacctgcctg ttctccgata tcgttctgcc caccgccacc tggtatgaaa aagacgatat
     4321 gaacacctcg gatatgcatc catttattca tccgctttct gcggcggtcg atccggcctg
     4381 ggaatcacgc agcgactggg aaatctacaa aggtattgcc aaagcatttt cgcaagtgtg
     4441 cgtgggccat cttggcaaag aaaccgacgt ggtattacaa ccactgctgc atgactctcc
     4501 ggcagagctc tcacagccgt gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat
     4561 cccgggtaaa accgcgccga atattgtggc ggtggagcgc gactaccctg ctacgtatga
     4621 acgctttacc tcgctcgggc cattgatgga caaacttggc aatggcggta aagggatttc
     4681 gtggaatacc caggatgaaa tcgatttcct cggcaaactc aattacacca agcgtgatgg
     4741 cccggcacag gggcgtccgc tgattgacac cgccattgac gcttcagaag tgattctggc
     4801 actggcaccg gaaaccaacg gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat
     4861 caccgggcgc gaacataccc atctggcgct gcacaaagag gacgagaaga ttcgctttcg
     4921 cgatattcag gcgcagccgc gtaaaattat ctccagcccc acatggtccg gtctggaaag
     4981 cgatcacgta tcctataacg cgggatacac caacgttcat gagttaattc cgtggcgcac
     5041 gctgtcggga cgccagcagc tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag
     5101 cctggtggct tatcgcccgc ctatcgacac ccgtagcgtc agtgagatgc gccagatacc
     5161 gccaaacggc ttccaggaaa aagcacttaa cttcctgacg ccgcaccaga aatggggcat
     5221 tcactcaacc tacagtgaaa acctgctaat gctgacgctc tctcgcggtg gaccgattgt
     5281 ctggatcagc gaaacagatg cccgagaact gaccattgtc gataacgact gggtggaagt
     5341 atttaacgcc aacggcgcgc tgactgcccg cgcggtggtc agccaacgtg taccgccggg
     5401 catgaccatg atgtatcacg cccaggaacg cattatgaat attcctggtt cggaagtaac
     5461 tggcatgcgc ggcggcattc ataactcggt cacccgcgtt tgcccgaaac cgacgcatat
     5521 gattggcggt tacgcgcagc tggcctgggg ctttaactac tacggcaccg tcggttcgaa
     5581 ccgcgacgaa ttcatcatga tccgcaagat gaagaacgtt aactggctgg atgatgaagg
     5641 tcgcgatcag gtacaggagg cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt
     5701 aacctcgata aatgtatcgg ctgccatacc tgttcggtga cctgtaacaa cgtctggacc
     5761 ggacgcgaag gcatggagta cgcatggttt aacaacgtcg aaaccaaacc gggcattggt
     5821 tatccgaaaa actgggaaga tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat
     5881 ggcaagatcc gtccgcgtct gggcagcaag atgggcgtaa taaccaaaat cttcgccaac
     5941 ccggtggtgc cgcagcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat
     6001 ttgcatagca caccggaagg caaacatatc cctactgctc gcccgcgttc actgattgac
     6061 ggcaaacgga tggacaaagt gatctggggc ccaaactggg aagaactgct gggcggcgag
     6121 tttgaaaaac gtgcccgcga ccgcaacttc gaggccatgc aaaaggagat gtacgggcag
     6181 tttgaaaaca ccttcatgat gtacctgccg cgcctgtgcg aacactgcct caatcccagc
     6241 tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg aagaagacgg cattgtgctg
     6301 attgatcagg ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa
     6361 atctacttca actggaaaag cggcaagtca gaaaaatgta tcttctgtta tccgcgaatt
     6421 gagtcaggcc aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc
     6481 gtgctgcttt acgacgccga ccgcatcgag gaagcgtcga gcaccgagcg cgaagttgac
     6541 ct
//
LOCUS       DAJTRN010000161         6434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRN010000161 DAJTRN010000000
VERSION     DAJTRN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..218
                     /locus_tag="OO328_003754"
     CDS             <1..218
                     /locus_tag="OO328_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932155.1"
                     /translation="QYSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLA
                     EWVREQLNKKHSNVVACALANKLARIA"
     gene            complement(557..1327)
                     /locus_tag="OO328_003755"
     CDS             complement(557..1327)
                     /locus_tag="OO328_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932156.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1372..1806)
                     /locus_tag="OO328_003756"
                     /pseudo
     CDS             complement(1372..1806)
                     /locus_tag="OO328_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1818..2039)
                     /locus_tag="OO328_003757"
     CDS             complement(1818..2039)
                     /locus_tag="OO328_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932157.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2040..2723)
                     /locus_tag="OO328_003758"
     CDS             complement(2040..2723)
                     /locus_tag="OO328_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5932158.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2787..3098)
                     /locus_tag="OO328_003759"
     CDS             complement(2787..3098)
                     /locus_tag="OO328_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932159.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3095..3997)
                     /locus_tag="OO328_003760"
     CDS             complement(3095..3997)
                     /locus_tag="OO328_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5932160.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4437..5396
                     /locus_tag="OO328_003761"
     CDS             4437..5396
                     /locus_tag="OO328_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5932161.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5396..5791
                     /locus_tag="OO328_003762"
     CDS             5396..5791
                     /locus_tag="OO328_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5932162.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5974..6354
                     /locus_tag="OO328_003763"
     CDS             5974..6354
                     /locus_tag="OO328_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5932163.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1759 a         1667 c         1560 g         1448 t
ORIGIN      
        1 ggcagtatag cactggagga aaatcgactc tgttgggtat cagtaagcga ggtgataaaa
       61 atttacgccg gttactggtt cagtgtgccc gctcattcat gatgcagctt gaacgccagc
      121 atggaaaact tgctgagtgg gttcgggagc aactcaacaa gaagcattcg aatgtggttg
      181 cctgcgctct ggcaaataaa ctggcccgaa ttgcctaggc aatcactact cagcaaaaag
      241 aatatcaggc ctgatatata gatgctgaca aaactcaatt agcttattgc aaacaataag
      301 atacaagtta ccgtctggtt ttgcgaagat tgattattga tgacatgaac ggcaaatcga
      361 cctgattaaa aacctaacag gggaaaatgg ctcactgaag ccgaggggat ttttaggatt
      421 atcaggtgcg gaactcatcg aggcgcgggc atcccttgcc cacagagacg ccggatagat
      481 ttaagcaagc ccaccattaa tcaaaaatca gtgttgcaaa aacgggggtg accatagatt
      541 ttccccgcca tcgtcatcat ttccggattt ttgccagggc atccgccatg ctgcggattt
      601 cggcccgggc ttcccggaca acttcccgca gaacaccacg gtcaaaccac cggcggggcg
      661 gttcatccgg tatacgcagc acatcgcagg ccagcccact ggacaggata tgctcaatac
      721 aatcgctgac cagatacatt tccaccgccg aaaaacgtgc gccccggttc aggatggcat
      781 cgagcaccac gggcaggttt tcatcgtctt catcgagcca gcaggggtga tcgctttcct
      841 tcacgaccag gcgaaagcgg cgcgtctggc gggtcagcaa cgtctgaccg cttgtggtca
      901 gcgccgcccg gatacgccgc gcatcttctg ccccccggca actgacactg catactgcat
      961 cgcgcaccgc atcaagaact gccctgattt cgtcttcggt cagccattca ccggcatacg
     1021 ggaaagacac ctgcgttccg tccgccggaa gtaccggcag cgactgccac tgactgccgg
     1081 acagaacaag ttccgctccg gttccggtca gtttctgaat ggcggaggcc aggtgcggcg
     1141 gcagcacgcc atcgtggcgg ttaacgagta acgtcgccag ccggggcgca tccagccgca
     1201 cgtaaatgct gatccagccc ggatagttcg ggttacattg gtcaacatcc gtctcttccg
     1261 gccagccctg cgccagcagc aggttacggg cgtgacgaca aatcagagca ttcagcgttt
     1321 cattcataat tcatgaactc cctgtatcgc cgcccacagg cggcgataac atcagtcgtt
     1381 ttcgtgtgtg ttgtttttca gcaggctgcg ggcctgatca accatcgcct gtcggcgggt
     1441 aacgtattcc gggaggggga aaccgtgatc ccgggcagcc cattcacgtt tccacagtgc
     1501 ctgttcagcg atctgagtca gggtctccag cagttcagca gggacggtca cctggcggtt
     1561 ttcgcgcacg ttgctgataa tctccatcgc cgcggcggaa gaaataccgg actcaatccg
     1621 ctagtcttcc gggatgtcct ccaggtgcgc caggacggtc ctgatttcat gatcggacag
     1681 ggaaatatcc attccgtcag cgagggcctg aatctcttcc ggggaccaga taaccagcat
     1741 cagcggcgtg tctcccgaat actttgctgc cagcgcccgg catagcgttt cgcatgttcc
     1801 gtacatactc tctcccctca atctttgaag caggaatcca gttccagaat ttcggtatat
     1861 gcctccatca agaggcggtt tgcgcgaaac aacgtctgac gcaccagacg ttccggcagg
     1921 gtgtcagcat taaagctgta ccgccgctcc atctcattca tggttgtacg caggtcgcac
     1981 atggcattgg ccacctccgc cacttcggcg cgaagttttt cgtgtccggc atagttcatt
     2041 tacgcagcct ccggcataaa ccagtcatca ttcgcggccc cctgctgcat ggcccgctgt
     2101 acggcagcca gtcgttgctg tccggcacgg tgatactgct caagcagctc gataccgata
     2161 taccggcgtc cggactggag ggcggcgacg caggttgaac cgctgcctgc aaacgggtcc
     2221 agcactatcg cattcgggtg tgtgaagctc tcaatcagcg gttgcaggct ggtaacgggc
     2281 ttttccgtcg ggtgatggcg gttgcccgaa tatttccagc ccaacacgtc cggcagcggg
     2341 ttttgtggca gacgtggacg gccttttgcc aggatgtagg cgcattcgtg gcgatagccc
     2401 acatatgcgg ccttcgatgt gtagtttttg gtgaagacca ggtgaccaac aacgctgaat
     2461 cccgcatttt tccaggcaga cataaagcga tcgacgcggt tccagccata gaagctcacc
     2521 atcagcgcgt cttttttgag tacgcggtac atttcattac aggccggttg cagccattca
     2581 tcagttttat caccagcaat ggtacgcccc tgacggtcac gaaaaccgac gagatatggc
     2641 ggatcggtga ggatgaaatc aatggcgtta tcaggaatac gggccataac atcgatgcag
     2701 ttaccgagga caaaacggga cataacagat ccttctctta cagagggcac ccctgtggtg
     2761 cccgttcccc gctgcctgtg ggctgttcat cgcttccggt aacgggtcag gccgaccgtg
     2821 gaataccgca gccgtcgtct ttttgacagc aaggttatgc cgcgaaagcg gcttgaaggg
     2881 cgttcatcag aaaagggcga cgggctggcg tacactgtca ccgacaccag ccgggagcgg
     2941 taaaagcgcc cgtcccgtgc ccgccggacg cgggacactg accacaggca gcgtttatgc
     3001 cgcgccgcgt cagccggcgc agtaaaaaga ggagcacggc gacatccttt gtcggaaaag
     3061 aaacgggcgg ttgcccgccc gtctccgtca tacttcatcc gccataatgc gccacattgt
     3121 cgactatcca cgcaggtccg gtgacttccg gctgctcatc gtcatctgtc ggggaagacc
     3181 attcaagttc cccccacagc acattgacga gctctccacg ctcatacaac tgccagccac
     3241 agaaatcaca gccctgttcg gcataccagt gctccagcgt gcagctgaaa cggcggctta
     3301 attctgcaat aacgtcgctt tccggctgac accacggagt gtcgaaatcc acctgaatga
     3361 agttttctcc ctgactgctg atattcaggt catatccaca aggccacttc acgccatacc
     3421 gttccgtata ccagtgatat gcagaaggaa caccgttcag aacaccacca ttaaagccgt
     3481 tcacttcaac atccaggcgg gtcggcagaa ccgtcagcag gtcaaacggc atggtttttt
     3541 ccggcactac gttgtcacac agacggttcc accacaccga tacatcctcg ttaccccata
     3601 tgtcacacca gtcacctctt tttgcggtga aatgcacagt gatggtttcc ctggcatcat
     3661 ccggtaatcc ttcccaacga cgctggccag taccgctctg taaccagagt tcatgcagca
     3721 tcaggcagtt ttgttcatcc agcaacacac cgttctgcag gtgtgtcagc cagcgggtga
     3781 acgcgatatt ttccgtcgat acagcaccac gtccggcagc cgtcactccg gggcactgtt
     3841 cagagcggat attttcagtg atctgcagaa gtccggcact tccggccaga aacagctgaa
     3901 taccttcatt tgtggcgcgg cgataaagcg gtgtgactgc accgctggcc agtcgtttaa
     3961 tctcagcgat ctgtgccggc tcaccaggaa aatacatacg attcgagcac cagtttggca
     4021 tgggaattct cctctcacat gcggcctcca tcaggccgtg ccgcacctgt aaccgggcga
     4081 ccgcatacga atgcaggtgc ggccccgaag ccgcagcgcg tggaggagga atgacgaaac
     4141 gtgaccgggt gaggaggccg gcgacagcga cacacttgca tcggatgcag cccggttaac
     4201 gtgccggcac ggcctggtta aatagtatcc gtctcagttc gtggcgcaaa atgagaaacc
     4261 aataagaact caaaatgaac cacaacaaga acataaagag actctaaaag agcaacaccg
     4321 tttgacatag actcaaaatg agtcacaata agaaacaata aaaaactcaa aaagatgcac
     4381 cgacataaag gatgctcaat ttgagataaa cacaaactca aaacgaggca aaaagaatgc
     4441 taaaagtatc atgcgatgat ggttcaacta acgtaaaact ggcctggctt gaagatggcg
     4501 aggtaagaac cagtctgtca ggaaacagct ttaaagaagg ctggaacccg ggactgttta
     4561 atgccggcaa agtttacaat tacgtcgtgg atgaaaaaaa atacacctat gatttgggaa
     4621 gcactgctgt cataggtaca acacatgtca gttatcaata cagcaccacc aatctgcttg
     4681 ccatacatca tgcactgctg acatccggcc ttcagccaca ggatgtagaa ctgacagtaa
     4741 cattacctgt gaccgaattc tttgataatg acaatcaacc aaatgaagaa agaatagaaa
     4801 gaaaaaaggc aaatgttctc cgtgagatat ctctgaataa aggggaaaca tttaaaatta
     4861 aaaaagttaa tgtaatgcct gaatctttgc cagctgcatt tgaatcacta aaaaaagata
     4921 aagtaaacaa acttgaacgc agcctgatta tcgatcttgg aggaaccact ctggattgtg
     4981 gtttaattct cggtgcattt gaaggcattt ctgaaatccg tggctattca gaaatcggga
     5041 catcccgcat cactcatacc gtcatgaatg cgctgacgaa agcctccaca ccatgtaatt
     5101 attttattgc tgatgagtta ataaaaaaca gacacgataa tgaatatctc cagacattaa
     5161 taaatgatgt agctgaaata aaaaacattt ctcatgtaat agacagagaa gtgaaatctc
     5221 tggcagaaag catcaggcag gaaatttcaa cattctcagg aatgaaccga atctatctca
     5281 ctggcggagg agccgagctt atttaccctc acataaagca atatttcccg aatctgaaag
     5341 tgaacaaggt ggatgaacct cagtttgcat tggtgaaagc aatggtgcat gcgtaatgga
     5401 aagcagtgat ccaaaaaaaa gaaagaaagt tgtggcatac ctgcacccgg cactttatcc
     5461 gcaggacaac ctgacacaac aaacaataga ctctctccca gtacaaatgc ggggagattt
     5521 ctacagacaa tcccttattt gcggagcagc tctttattct gttgcccccc gactgttaac
     5581 gctaatcagc gtttttttca gtgagaaaat cactgcagaa aatctggtga aacttataga
     5641 acaaacaaca ggatacacat ccacatcaat tgatataagt gtgttaaaaa acatcataga
     5701 ggcatcttca gaaaacaaat cagagagcat cacttcaaaa gatgatttcg aagaacaaac
     5761 cagacgcaat ctttctatgt taaaaaaata ataaaagaag agcaggttca gtcaattacc
     5821 tgctcttctt ttatacatac taaaaaatca gatttttacg aatattcaga ttatcataaa
     5881 aaatgcaaca gacaggaatg caataatttt gcattatctc gttgcttgta atgcaaatat
     5941 gcattattgt cagtgtgtga caataacaga gagatgacga tggcgaccat taacgcacgt
     6001 attgatgatg atataaagaa ccaagcagat gaagtgctga agctgatgaa tatcagtcaa
     6061 actcaggcta ttgcagcttt ttatcagtat attactgaac aaaagaaact gccatttgtg
     6121 atcacctcaa ttgtaaaaac gccgcatgat ttattacgtg aaagtacaga catgttagct
     6181 gaagctcttg cagtgatttc caatctacag gtgtggactg aacagcagga tgggattgga
     6241 aaagcaaagc taatggagta ttaccgccgc ctcgacgcac tgtactgttg tgcaaaagag
     6301 aaaataggtc ttctttcaga taaccgggat gctgaactag gctgtgtccc ttaatcatct
     6361 gagttatagt aactgtctgt ttacacaaca tattgaacta tggctcgcta cgaccttccc
     6421 gatgaggcat ggac
//
LOCUS       DAJTRN010000162         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRN010000162 DAJTRN010000000
VERSION     DAJTRN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO328_003764"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO328_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5932164.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO328_003765"
     CDS             2089..2238
                     /locus_tag="OO328_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932165.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO328_003766"
     CDS             complement(2983..3423)
                     /locus_tag="OO328_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5932166.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO328_003767"
     CDS             3641..4030
                     /locus_tag="OO328_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932167.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO328_003768"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO328_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO328_003769"
     CDS             complement(4585..5421)
                     /locus_tag="OO328_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5932168.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO328_003770"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO328_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5932169.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1431 a         1618 c         1641 g         1510 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aacaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTRN010000163         6197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRN010000163 DAJTRN010000000
VERSION     DAJTRN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..815
                     /locus_tag="OO328_003771"
     CDS             <1..815
                     /locus_tag="OO328_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5932170.1"
                     /translation="YLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVF
                     TGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLW
                     RFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSRKSV
                     LFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGS
                     SVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            858..1361
                     /gene="yeaK"
                     /locus_tag="OO328_003772"
     CDS             858..1361
                     /gene="yeaK"
                     /locus_tag="OO328_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5932171.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1362..1466)
                     /locus_tag="OO328_003773"
     CDS             complement(1362..1466)
                     /locus_tag="OO328_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932172.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1636..2082
                     /locus_tag="OO328_003774"
     CDS             1636..2082
                     /locus_tag="OO328_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5932173.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2039..2857)
                     /gene="nimR"
                     /locus_tag="OO328_003775"
                     /pseudo
     CDS             complement(2039..2857)
                     /gene="nimR"
                     /locus_tag="OO328_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            2954..4134
                     /gene="nimT"
                     /locus_tag="OO328_003776"
                     /pseudo
     CDS             2954..4134
                     /gene="nimT"
                     /locus_tag="OO328_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4189..4536
                     /locus_tag="OO328_003777"
     CDS             4189..4536
                     /locus_tag="OO328_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5932174.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4558..4812)
                     /gene="yoaF"
                     /locus_tag="OO328_003778"
     CDS             complement(4558..4812)
                     /gene="yoaF"
                     /locus_tag="OO328_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5932175.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4995..6020
                     /gene="dgcP"
                     /locus_tag="OO328_003779"
     CDS             4995..6020
                     /gene="dgcP"
                     /locus_tag="OO328_003779"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5932176.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6053..6151
                     /locus_tag="OO328_003780"
     CDS             6053..6151
                     /locus_tag="OO328_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5932177.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
BASE COUNT         1491 a         1495 c         1518 g         1693 t
ORIGIN      
        1 attatctcga tctcaatgac cgctatgtct atttttatga gccggttaat gttgaatact
       61 ttgcgatgaa taactggtcc ttcctgcagt caggaagtat tggcatcgat cgcaaagata
      121 ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat ttaccaggat cagcgtacta
      181 aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc agggcagcta aaagggattg
      241 tgctgctgga tattaacaaa aacaatttgc ggaatatctt ttacactcat gaccgccctc
      301 tcctctggcg ttttctcaat gtcacgctaa ccgataccga ttcggggcgc gacattatca
      361 tcaaccagag cgaagataat ctgttccagt atgtcagtta cgtccatgac ttacctggcg
      421 gcattcgtgt ctcgttatcc attgatattc tttactttat cacgtcttcg aggaaaagcg
      481 ttctgttctg gattttgacg gcgttaattt tgctgaatat ggtccggatg cacttccgtt
      541 tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat gactggactg tataatcgca
      601 aaattttaac ccctgaactg gagcagcggt tgcagaaact ggtgcaatcc ggttcttcgg
      661 tgatgtttat tgcaattgac atggacaagt taaagcaaat aaatgacacc ctcggtcatc
      721 aggaggggga tttagcgatt acgttacatg atgcgtataa agcttccgat gagcgtttat
      781 atgtcaataa gcagaacaaa aacagccgtt catgataacc ttctgtggtt gtttgcttgt
      841 aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat cagcagttaa
      901 tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt tacccatgaa gcggtaggta
      961 aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg ccagggtgct aaggcactgg
     1021 tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc gcagccgatc
     1081 aacaggccga tctgagccaa ctcgccagcc atataggcgg attacgcgct tcgttggcca
     1141 gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct
     1201 tccatccaaa actcaaactg gttgccgacc ctctactctt cgaacgattt gatgaaatcg
     1261 ccttcaatgc agggatgctg gataaatccg ttattttgaa aaccgcggat tatttgcgca
     1321 ttgcgcaacc agaactggtc aatttccgcc gcactgcgta gctagcctat ccgttcaata
     1381 agcagaacgg acaaaaccag cacaaccgca atagcaaaaa acgatgatgt gataatcagt
     1441 gtctcgacga acatttgatc gttcatagca tgcccccggg agatagctgt tttcacgtta
     1501 ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc tgatttttta tgcaaaaaac
     1561 gtaaagattt tctacttcct tcctgcagca agcataaagt aagcaggctt attatttttt
     1621 ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg
     1681 ggctttatca gtcataacac cactgtcgcc gtttcaattc tggtgttgat cattgtccgc
     1741 gtcacaccgt taagcacctt tttcccgtgg attgaaaaac aggggctaag tatcgggata
     1801 atcattctga cgattggcgt tatggccccc atcgccagcg ggacgctacc gccctcaacc
     1861 ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc
     1921 tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc agccgcaact ggttgctggg
     1981 ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg gcgtaccggt tggcccgctt
     2041 attgccgcgg gtctggtttc gctgattgtg gggaaacagt agttaatccc gcaatatatc
     2101 ggcctggcgt ttgccccaag cctttcttaa acatagtaat aaaggccgtt gttgaatcat
     2161 accccagcgt atgagcaact ttctgtacag tatcgccttt cactaatccc tgaagcgcca
     2221 taatcagctg taactgctga cgccactggc gaaaactcaa ccccgtctct ttcactatca
     2281 ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc ccattgcccc aatgcccccc
     2341 actcgacggg ccctttcgcc atcatctcca ccatcgtgcg gattttagga tgagaagaaa
     2401 cgggcaggtg cagctgttgt tgtggctgct gagggagttc atcaaacagg acctgaatta
     2461 atctgcgggt catcggctca gcacgctgag tcgtcgtcct gtttgccagg gttaaaatca
     2521 attcccggca taagggcgat attttcaatg tgcaacatgg tcggcattgt cactgcgctg
     2581 ggttcgataa acagaaaaca gagttcagcg ttagctgtca cttgattgct atgcttcacc
     2641 ccgcccggta tccaaaccgc atattgtggc ggcaccatcc acaaagcatt ttccaccgta
     2701 caggtaattg caccatgtaa tgccagtatc agctgtcctt tgcggtgctg atgtacagga
     2761 ctggttaact catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg
     2821 tgggttcata gccattgaga ttcaacctgt gcatcatttt gtccgaactt agcgataatt
     2881 tgtcatttta gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa
     2941 caatgagaag catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat
     3001 cgctggcatt ctgatgattg ccacaacatt acgcgtcacc tttaccggcg cagcgccgtt
     3061 actggatacg attcgttccg cttactcgct gacgacagcg caaaccggct tattaaccac
     3121 cctgccatta ttggcctttg cgctaatctc acctctggct gccccggtgg cgcgacgttt
     3181 tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc tgtgcaggta tcgcaattcg
     3241 ttctctccct tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc
     3301 attaggcaat gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag
     3361 acttaccggc gcatattccc tgacaatggg agctgcagcg gcactgggat cggctatggt
     3421 cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg ttgttcatgc tgatgtgttt
     3481 tcctctgctg gctctttttt tatggctgcc acagtggcga agtcagcaac atgcaaattt
     3541 gagtacctca cgcgccttac atactcgggg tatctggcgt tcgccacttg cctggcaggt
     3601 aacattgttt cttgggatca actcactggt ctattacgtg attattggct ggcttccggc
     3661 gatcctcatc agtcacggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca
     3721 actagccaca gcagctcccg gtttgctgat cgcacttttc ttacatcatg tgaaagacca
     3781 gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg ggcgcggttg ggctctgctt
     3841 tatgccagcg cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat
     3901 gatactgggg ttgacgttca ttggtcttcg gcaagttctg cgcatcaggc ggcggcactc
     3961 tcggggatgg cacaatccgt cgggtatttg ctggcagcct gtgggccgcc gctaatgggt
     4021 aaaatacacg atgctaacgg taactggtcc gtaccactga tgggtgttgc aatactttca
     4081 ctactgatgg cgattttcgg actttgcgcc gggagagaca aagaaattcg ctaatatccg
     4141 gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa caaacacgat gaatattcag
     4201 tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc
     4261 ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc
     4321 acgccgtcaa cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt
     4381 cgcgagcaat atcgcgcaga actggcgcaa cacgagcaag aaggaaagcg gctggcggac
     4441 atcgccaaaa aacagccgct gaccctgctc tacgcagcaa aaaacaccac gcagaaccat
     4501 gcgctggtgc tggccgactg gctacgtagc ttgtgatttt agtacggcat ccggcggtta
     4561 tttttcacca gccggatggt cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc
     4621 cggtaatttg caattgttgc tgaccccttg cgctgtctgc actggaatga gcgtcccgcc
     4681 cttctgctgg caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtgc
     4741 ttctggctga gaaggggttg aacaaccagc caggaccagc aagcaaggca gaacaaaact
     4801 gataattttc atttattgat ctcacatatt tatccaagct tagagtatcg cggtatcgtt
     4861 ttgttttgca gcactatttt tattacattc actcaaaaca tattacgtct tgtttcatct
     4921 ttgttgatga tgttttatca tgcctgcaaa gattaaataa tcagcactta cccgccgtat
     4981 cctggagttg ttccgtgtca gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag
     5041 aacagtcact ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata
     5101 tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg
     5161 cccgtaacag ccagaaaatg cacatcccgg agaattttac cgtcttgtgg gattactcgt
     5221 tatgcaaacg cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg
     5281 gcgactgtat tgcggcacgc aatcttggca tcaccacatt tctgagcacg ccaattcact
     5341 taccggatgg atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga
     5401 gtgaacgcgc ggaacaggtt ttacagttat tcgccggact gattgcacaa tatattcaaa
     5461 aagaggcact ggttgaacag ctgcacgaag ccaatgctgc gctgattgcg caatcgtata
     5521 ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt tgaaaatctg acgacgctgt
     5581 tttccctcgc ccggcatctt aaccataaga taatgatcgc gtttatcgat ctggataact
     5641 tcaaattaat caatgatcgt tttggtcata atagtggcga tctgtttctc attcaggttg
     5701 gcgagcgcct taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggtggtgatg
     5761 agtttttggt tgtttcacta aacaacgaga atgcggatat ttcgtcgctg cgagaacgta
     5821 ttcaacagca aatacgtgga gaatatcact taggtgatgt tgatttgtat tatcccggtg
     5881 ccagtcttgg catagtagaa gtcgatcctg aaacaaccga tgcagacagt gccctgcatg
     5941 ctgccgatat tgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc
     6001 atccagcgct acattcctga ggcgtattca catccttttg attggtgata acatgcgaat
     6061 cggtattatt tttccggttg taatcttcat tacagcggtc gtatttttag catggttttt
     6121 tattggctgc tatgctgccc cgggagcata aagatgaaaa aaacaacgat tattatgatg
     6181 ggtgtggcga ttattgt
//
LOCUS       DAJTRN010000164         6136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRN010000164 DAJTRN010000000
VERSION     DAJTRN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO328_003781"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO328_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5932178.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO328_003782"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO328_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5932179.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO328_003783"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO328_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO328_003784"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO328_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1618 a         1442 c         1450 g         1626 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagata
//
LOCUS       DAJTRN010000165         6011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTRN010000165 DAJTRN010000000
VERSION     DAJTRN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            525..1703
                     /gene="ltrA"
                     /locus_tag="OO328_003785"
     CDS             525..1703
                     /gene="ltrA"
                     /locus_tag="OO328_003785"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5932180.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1786..1965
                     /locus_tag="OO328_003786"
                     /pseudo
     CDS             1786..1965
                     /locus_tag="OO328_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1995..2477)
                     /locus_tag="OO328_003787"
                     /pseudo
     CDS             complement(1995..2477)
                     /locus_tag="OO328_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2733..3713)
                     /locus_tag="OO328_003788"
     CDS             complement(2733..3713)
                     /locus_tag="OO328_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5932181.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3713..4912)
                     /locus_tag="OO328_003789"
     CDS             complement(3713..4912)
                     /locus_tag="OO328_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5932182.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5234..5572)
                     /locus_tag="OO328_003790"
                     /pseudo
     CDS             complement(5234..5572)
                     /locus_tag="OO328_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5639..>6011)
                     /gene="istB"
                     /locus_tag="OO328_003791"
     CDS             complement(5639..>6011)
                     /gene="istB"
                     /locus_tag="OO328_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5932183.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1639 a         1378 c         1423 g         1571 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtagaggtgg gccctccggt cagggtttac
       61 aggagcacag accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt gggttcggga agctgaacga aagtaaactg
      181 tccgatgaag catcgaaatc ttagatgatg ccaaaatggg gccgtacagc cgtcccctga
      241 taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg cagcaggatc
      301 ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc gggaaacagg
      361 ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg aagggatggg
      421 gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga tgcaacgaaa
      481 atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc gtcgcaacaa
      541 gggagccccc cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa cgtgatggca
      601 atctatacaa gatctggaac cggctatgtt cgggaacatg gtttccgcca ccagtgctgg
      661 aaaaacggat cccgaagtcg aatggcaagg aacgcattct ggggatccca acagtatccg
      721 atcgaattgc tcagggagcg ataaaacttt tcatggaaga aaagcttgac ccgattttcc
      781 atgctgattc atatggttac cgaccaggca aatcagctca tgacgctctg aaacaatgtg
      841 ccatccggtg ctggcgttat agctggatac tggaagtgga catcagtgcg tttttcgacc
      901 atgtgagaca tgatctggtg ctcaaagctc tggaacacca cgggatgcct aaatgggtca
      961 tcctgtactg tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat ggagaattaa
     1021 taacccgaac acgaggcact ccgcagggcg gggtcattag tccgctactt gctaacttgt
     1081 tccttcacta tgcatttgat ttgtggatgg aaagagaata tcggggggta ccgtttgaga
     1141 ggtacgctga cgatattgta gtacattgtt cacgaatgag tgatgcaaca aggctaaaga
     1201 acaggttatc ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg aaaacgaata
     1261 tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac gagtttcacc tttctcggat
     1321 atgacttcaa agtgcgtacg ctgaagaatt tcaacggcga acggtaccga aaatgcatgc
     1381 cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac aatcaagaag tggcgtatac
     1441 atcgctcaac agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg atagtgagag
     1501 gctggatcga gtactacgga aagttctggt ccagaaattt caactatcga ctgtggagtg
     1561 caatgcagtc acgtctgctc aagtggatgc agtctaaata cagactttcg aaccggaagg
     1621 ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa gctatttgtt cactggtatt
     1681 tactacgtgc atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc acgtacggtt
     1741 ccgtgagcag ccggggagtg aaattctctc cggctgactc gactcgcggg ttccgacagc
     1801 gatgacgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     1861 gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc aggactggcc ggcaaaccgg
     1921 gtacgtgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc
     1981 tgacgagccg cttacttttg gcgtaattct gctgcgcctt tactgaacac aatgccgtat
     2041 tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc tttccattga aaccaaccga
     2101 cgcatattca aaaaagtctg gacgtcctgt tgctccagag tttttattgg tacgggtcgt
     2161 atagttccat gttggcaggc ctcagctatt gcctgtgcgt cgttataatc atttttctgg
     2221 caccgctgat aagctttaac atgttgtgcc ggaagcaata ttacatcatg gtcgagtttc
     2281 ctgatttccc gggcccagta atgagatgcc ccgccagcct ccatcgcaac agtcatttta
     2341 ggctgttgca ccagccagtt aagcagttga ttgcgagaaa gtttggtctt tcctgcgggt
     2401 ttgccatgag cattgatact gaaaagataa aagttcgttt tagccagatc aatgccggtg
     2461 attttctgtt cagacatggt atcccgctta cttttgttgt taaagtttat ccatcatggc
     2521 gcaattgaag gctgccgtga aagaggggga agactatctc atcggttact gttaaacggc
     2581 taagagaaac tatctttttc aagcaaagaa gatacggtga aacaggcgat ttcaccgtga
     2641 aatactgttc aaaaaaaacg ctaataaaag caaaatttct tttaaaacag ccaattaaaa
     2701 actggcgagt tgctggcgaa aaacttgaat tttcacagcg aattcagatt atcttttagt
     2761 acaagagcaa tcatacgatc taacttatcc tgaacttcca acggtaatct gccgaattca
     2821 tatgagaacg tacgtccctt tactcgtttt cgagcaaatt tatctttact atcaaatttc
     2881 cataacaaag ttacaaccgc tttatccttt actcctgaat caagcaatga agacatttcc
     2941 ttggttataa gccgcataat actgttttta acctcgtctt cagaaagatt gtcatcagca
     3001 gtaataagct caatactcgg cgagatattc ttcacaactt catcaataga tatatcaccc
     3061 agcctcagtc gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt cagctcagac
     3121 tgaacaggaa aaagagacac aaaatcttta ggtacggacg ctgcttggag ggcacgagtc
     3181 accttagctg ccgacaatcc ctctctttcc gcaatttgct tctggttcat accggcctct
     3241 ttgagacgta ctaaacgcaa cccaatttca cgaatattat gttcaagtga cgtctgcaaa
     3301 tctttcgcca gatgctgagc ttcagaaaca gtaagctcat catccgttac aagtacacgt
     3361 aatccaacct tgcatagcaa agctgctgcc cgacgacggg aaccatccaa aatctcaata
     3421 agatcgccag tccgaatccc aatacatgga tagaattgtt gtaaacgtat agttctggtg
     3481 atgtctttta gtgagtccgg cgtcagggct gtctgttcgc ggccattcac ctcttcaacc
     3541 acaaaagttt gagtatccac tttgtcatga tcaaccttga tctcagtaaa ttttgccttt
     3601 cggccagtat tgagggtaaa tacatgaact ggagccgata tttcttcagt attattcagg
     3661 atatttgtat tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc catcagtagt
     3721 tggcccttat aaattcaata cgatcaaaaa ctgctttagc gaaatcttct gctgccatac
     3781 gtgcattttt caaagcttca ccactgccaa cataagtaac aggattcgca gatatcacgg
     3841 tatcaaacga ctcgccgctt ctctcaaaac catcaagacg tggcattgaa acatcaagca
     3901 tgtcaccacc gaaaatctct tttgtcaggc tatggcagta tttatgatct gacttattcg
     3961 ccagtttcga catgaagcca atatttgcct gcaaacggca tgaacatcca tcctgttcaa
     4021 taatctgtac aagttcaggt aaacgagcca aatatttaag cgtggaatgg aaatcaactt
     4081 gtgctggcgg caccggtgta aacatgatat ctgcggcagc aatggcattt ttcaaaaatg
     4141 cgtccagatg cgggcctgta tcaataagga tgaagtcgaa atcatgcttc agcttatcaa
     4201 taatattctc acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc tcgcacagag
     4261 tatcccagtt tgatgcaata aaggcgtcgt caatagaagc cggcataaca taaacgccag
     4321 ggataactga cggaacgata aaatcttcca gtaattcctc acgactcacg ttctgcaaca
     4381 tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg caagtagttc agaaacatag
     4441 tggctgatga ctggggatct aaatcaagga gcagaatacg taaatcttct gacagcaggt
     4501 gaggatgagc acgcagggcg tgagctacgg aaacactact cacggtttta gacacaccgc
     4561 cttttagact accgatgaat atggaatatg cctcactata acgatcgcga tatttaggaa
     4621 tgccacgatg tgcatagata tcgataatgt tctggatggt taatgcatat tttgtcgcgg
     4681 ttcctgctgg gcgtttttca aactgatagc cctgagcctc catctcaccg actgcaccat
     4741 ccacgcttgc gcgcgttaat tttggaagct tagctacagc agactttgaa tagacttgat
     4801 aaaaaacatc agcctgaaac tcctgctttt gctcctgaat ctgttttgtt agggcgagca
     4861 acattttatc tgcacgctgc gcaaccttac ttaactgctc aaaactagtc attccccctc
     4921 cgtgctatgt aggattttaa cttattacaa aaaaaactgc attagcaggg taatgcatgc
     4981 aagtacatat tgtacatgct tatattaaaa atgcgcgcat tgctgattta ttaatcatcg
     5041 gaatgaatat tactgtaaga tagtatgcct ttccgggaga aagacgctga tttcccccga
     5101 ataatccagt tcaccaccac actccggaca acagctttct tcaggctcca gatggtgtat
     5161 ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt gactgtcgca acaggcgggg
     5221 gtagtgcttc caatcagtag aacatgtgct tttcaatgaa ggagccgatg actttttcgt
     5281 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
     5341 ggagggtccg gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc aggtgtttct
     5401 ccttcggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga
     5461 acggagtcaa cagggctaac agttcccgac aggtctcatc attccgtggc ccgaaagtgt
     5521 aagccaacac accgccagtt ttagtgttgt acgcatacca gagccagtgt tgtgtcaacg
     5581 acggatgaaa agtgatccac ttatatctcc accaacggcc caatattgat ccaccgtttt
     5641 actcaggatt agcttctgct ataaccccgg cctttcgttt ctgtctgagt cgatagcttt
     5701 ctcctttgat ttgaacgaca tgtgagtggt gtaagatacg gcccagcatc gctgaggtca
     5761 gtgctgcatc accggcgaac gtttgatccc actgcccgaa cggtagattg gatgtcagga
     5821 tcattgcgct cttttcgtaa cgtttagcga tgacctggaa gaacagtttt gcttcttcct
     5881 gactgaacgg cagatagcct atttcatcaa tgatgagcag gcggggggcc attactccac
     5941 gctgaagcgt cgttttataa cggccctgac gttgtgccgt agataactga agtaacagat
     6001 ctgctgctgt t
//
LOCUS       DAJTRN010000166         5983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTRN010000166 DAJTRN010000000
VERSION     DAJTRN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..393)
                     /locus_tag="OO328_003792"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO328_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO328_003793"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO328_003793"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5932184.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO328_003794"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO328_003794"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5932185.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OO328_003795"
     CDS             5797..>5983
                     /locus_tag="OO328_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932186.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJTRN010000167         5885 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTRN010000167 DAJTRN010000000
VERSION     DAJTRN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            26..1516
                     /locus_tag="OO328_003796"
     CDS             26..1516
                     /locus_tag="OO328_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5932187.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1568..2725
                     /locus_tag="OO328_003797"
     CDS             1568..2725
                     /locus_tag="OO328_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5932188.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2691..2813)
                     /locus_tag="OO328_003798"
     CDS             complement(2691..2813)
                     /locus_tag="OO328_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932189.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3016..4701
                     /locus_tag="OO328_003799"
                     /pseudo
     CDS             3016..4701
                     /locus_tag="OO328_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4739..>5885
                     /locus_tag="OO328_003800"
     CDS             4739..>5885
                     /locus_tag="OO328_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5932190.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDN"
BASE COUNT         1718 a         1275 c         1235 g         1657 t
ORIGIN      
        1 aaagccaaat attatcgtac taaccatgga tgatcttggt tatggacaac ttccttttga
       61 taagggatct tttgacccaa aaacaatgga aaatcgtgaa gttgtcgata cctacaaaat
      121 agggatagat aaagccattg aagctgcaca aaaatcaacg ccgacgctcc tttcattaat
      181 ggatgaaggc gtacgtttta ctaacggcta tgtggcacac ggtgtttccg gcccctcccg
      241 tgccgcaata atgaccggtc gagctcccgc ccgctttggt gtctattcca ataccgatgc
      301 tcaggatggt attccgctaa cagaaacttt cttgcctgaa ttattccaga atcatggtta
      361 ttacactgca gcagtaggta aatggcactt gtcaaaaatc agtaatgtgc cggtaccgga
      421 agataaacaa acgcgtgact atcatgacaa cttcaccaca ttttctgcgg aagaatggca
      481 acctcaaaac cgtggctttg attactttat gggattccac gctgcaggaa cggcatatta
      541 caactcccct tcactgttca aaaatcgtga acgtgtcccc gcaaaaggtt atatcagcga
      601 tcagttaacc gatgaggcaa ttggcgttgt tgatcgtgcc aaaacacttg accagccttt
      661 tatgctttac ctggcttata atgctccgca cctaccaaat gataatcctg caccggatca
      721 atatcagaag caatttaata ccggtagtca aacagcagat aactactacg cttccgttta
      781 ttctgttgat cagggtgtaa aacgcattct cgaacaactg aagaaaaacg gacagtatga
      841 caatacaatt attctcttta cctccgataa tggtgcggtt atcgatggtc ctctgccgct
      901 gaacggggcg caaaaaggct ataagagtca gacctatcct ggcggtactc acaccccaat
      961 gtttatgtgg tggaaaggaa aacttcaacc cggtaattat gacaagctga tttccgcaat
     1021 ggatttctac ccaacagctc ttgatgcagc cgatatcagc attccaaaag accttaagct
     1081 ggatggtgtt cccttgctgc cctggttgca agataagaaa caaggcgagc cacataaaaa
     1141 tctgacctgg ataacctctt attctcactg gtttgatgag gaaaatattc cattctggga
     1201 taattaccac aaatttgttc gccatcagtc agacgattac ccgcataacc ccaacactga
     1261 ggacttaagc caattctctt atacggtgag aaataacgat tattcgcttg tctatacagt
     1321 agaaaacaat cagttaggtc tctacaaact gacggatcta cagcaaaaag ataaccttgc
     1381 cgccgccaat ccgcaggtcg ttaaagagat gcaaggcgtg gtaagagagt ttatcgacag
     1441 cagccagcca ccgcttagcg aggtaaatca ggagaagttt aacaatatca agaaagcact
     1501 aagcgaagcg aaataactaa accttcatgc ggcggatttt tccgccgcct tattgagcga
     1561 gatagcgatg cacgttacag ccaagccctc cagttttcaa tgtaatctca aatgtgatta
     1621 ctgtttttac cttgaaaaag agtcgcagtt tactcatgaa aaatggatgg atgacagcac
     1681 tctgaaagag ttcatcaaac aatatatcga agcgtctggc aatcaggtct attttacctg
     1741 gcaaggcggt gaacccactc tggctggcct ggattttttc cgtaaagtta ttcactatca
     1801 acaacgctat gcaggccaaa aacgtatttt taatacatta caaacgaatg gcattttatt
     1861 gaataatgaa tggtgtgcct ttctcaaaga acatgaattt ctggttggta tctcgatcga
     1921 tggcccccag gagttacatg accgttacag acgcagtaat tcaggtaacg gtacttttgc
     1981 aaaagtgata gcagccatcg agcgtctgaa atcatatcaa gtagagttta atacgttaac
     2041 cgtcattaat aacgttaatg tccattatcc tcttgaggtt tatcattttt taaaatctat
     2101 cggcagtaaa catatgcaat ttatcgaatt gctagaaacc gggacgccga atattgattt
     2161 cagtggtcat agtgagaaca cattccgtat cattgatttt tctgtgcctc ccacggctta
     2221 tggcaagttt atgtcaacca tttttatgca atgggttaaa aacgatgtgg gtgaaatttt
     2281 cattcgtcag tttgaaagct ttgtcagccg ttttttgggg aatgggcata caagttgtat
     2341 tttccaggag tcctgcaagg ataatctggt tgttgaaagt aatggagaca tttacgaatg
     2401 cgaccatttt gtctatccac agtacaaaat tggaaacatt aataaatctg aactcaaaac
     2461 gatgaacagt gtacaactga cagcgcaaaa aaaacggatt tcagcgaaat gtcagcaatg
     2521 tgcatataaa cctatctgca atggcggttg tcctaagcat cgtattacta aagtaaacaa
     2581 tgagactgtt tcttattttt gcgaaggtta taaaatcctt ttttcaacca tggtacctta
     2641 tatgaacgcc atggttgagt tagctaagaa cagagtaccg ctttaccaca ttatggatgt
     2701 tgcaaaacaa atggagaata attaataacg ctctctttta actccattct tagcctcctt
     2761 cttatgacct taacaaggca cccgacataa attcctgtcg ggtgtttcgt catcatcatc
     2821 ttcccttgcg aaaatcctgg cttacgtggt atgcgtcagg gcattagcaa tacccttgat
     2881 tatcaatagc ataaccataa ccactgaaaa ttaaaaattt ctatatattt catattgtta
     2941 aatgatgctt aaataaattt catcagtaaa tcattgccat agataatcat tggatggcac
     3001 aatcaatttt cgctaatgat aaccattccc attacgtttt gtatgttgat agcaaagtat
     3061 ctctgtctgc tgaagccctt ttggctgcgt aagaataata aaacttcagt gttgttaatc
     3121 attattatcc tcgcaatgat ccttggcgtg gttaaaattc aggtctggtt aaacgactgg
     3181 aataatgatt ttttcaatgc gctaagccag aaagaaaccg ataagctttg gcagctcgtt
     3241 ctttggttcc ctgccctgct ggggatcttt gtattaatat ccgcaaacaa aacatggtta
     3301 attaaactac tgactattcg ctggcgcgaa tggttaacag attactacct taatcgctgg
     3361 tttgcagata aaaactacta cttcacgcag atctacggcg agcataaaaa tacagataac
     3421 cctgaccaac gtatcgctga agacattctc ttactgatca acaaaacgct cagtctttca
     3481 tttggcttca tccagtcact tagcatgctg atcaccttta ccgttattct ttggcaaagc
     3541 gcgggtacgc tctcattcac tgttggtgga acagaatgga atatccaggg gtatatggtc
     3601 tataccgtcg tgctcattgt gatcggtgga actttattta cccataaggt gggtaaacgt
     3661 attcgtccgc ttaatgtgga aaaacaacgt agcgaagcga catttcgaac taatcttgtg
     3721 cagcataata agcaggcaga actgattgcg ctaagcaatg ctgagtctct acaacgccag
     3781 gagttgcgcg acaattttca taccattaaa gagaactggc atcgtttaat gaatcgtcag
     3841 cggtggcttg attactggca aaatatttat tcgcgctcgc ttagcgttct tccctacttt
     3901 ctgttattgc cgcagtttat tagcgggcag atcaatctgg gcggactgat gaaatcgcgc
     3961 caggcattta tgctagtatc gaacaattta agctggttta tttataaata tgacgaactt
     4021 gctgaactgg ctgcggttat cgatcgcttg tatgagttcc atcaactcac tgaatagcgc
     4081 cctacgaata agcctaaaaa ttgccaacat gcggtacaag tggctaatgc gagtattcgt
     4141 acgcctgata ataagatcat attagagaac ttgaactttc atgtttcgcc aggcaaatgg
     4201 ctattactga aaggctactc tggtgcggga aaaaccacac tgcttaaaac attatcccac
     4261 tgctggccgt ggtttaaagg tgatatttct tctcctgctg acagttggta tgtgtcacaa
     4321 acaccgttaa tcaaaagcgg cttactgaaa gagattattt gtaaagcact tcccctgccc
     4381 gtagacgata aatcgttgag cgaagtactg catcaggttg gtctgggaaa attggctgcg
     4441 cgtattcatg accacgatcg ctggggagat attctttcca gcggcgaaaa acaacgtatc
     4501 gccctggcac gattaatttt acgacgtccg aaatggatat ttcttgacga aactacctct
     4561 catcttgagg aacaagaggc tatacgctta ctgcgtttag tgcgtgaaaa actacccaca
     4621 agcggcgtca ttatggttac acatcaaccc ggtgtctgga acctggctga tgatatttgt
     4681 gacattagca cggttttata acaacaataa aagccctgct gtacggagat ataaataaat
     4741 gaagcgagtt cttattcctg gcgtcatttt atgtggcgct gatgtggcgc aggccgtcga
     4801 tgacaaaaac atgtacatgt atttttttga agagatgacg gtctatgctc ctgtccctgt
     4861 acccgtaaac ggcaacacgc attacaccag tgaaagcatc gagcgtttac cgaccgggaa
     4921 tggcaatatc agcgatctgc tgagaaccaa ccctgcggta cgcatggatt caacgcaaag
     4981 tacctcgttg aaccagggag atattcgccc tgagaaaatc tctattcacg gtgcgtcgcc
     5041 ctaccagaat gcctatttga ttgacggtat tagtgcaact aataacctga acccagcgaa
     5101 tgagtccgat gccagtagtg caaccaatat tagcgggatg tcacaggggt attatcttga
     5161 tgtcagctta ctggacaatg tgacgcttta tgacagtttt gtgccggttg aatttggtcg
     5221 cttcaatggc ggggtaattg atgcaaagat caaacgcttc aacgctgatg atagcaaggt
     5281 gaaattgggt tatcgcacta cgcgtttgga ctggttaaca tcgcatatcg atgagaataa
     5341 caagagcgca tttaatcaag gttcttcagg aagtacctat ttctctccag attttaaaaa
     5401 gaacttttat accttgtcgt ttaatcagga gctggctgat aactttggcg ttaccgccgg
     5461 tttatcgcgc cgccagtctg atatcacccg cgcggattat gtttcgaatg acggcattgt
     5521 cgccggtcgg gcacagtata aaaacgttat cgatactgca ttgagcaaat ttacctggtt
     5581 tgccagcgac cgctttaccc acgatttaac cttaaaatat accggctcca gccgtgatta
     5641 taataccagc accttcccgc agtctgatcg cgaaatgggt aataaatcct atggtctggc
     5701 atgggatatg gatacgcaac tcgcatgggc caaactacgt accaccgttg gttgggatca
     5761 tattagtgat tatacccgtc acgatcatga catctggtac accgaacttt catgtacata
     5821 tggtgatatt acagggcgtt gtacccgtgg cggattagga cacatttccc aggctgtaga
     5881 taatt
//
LOCUS       DAJTRN010000168         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTRN010000168 DAJTRN010000000
VERSION     DAJTRN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..411
                     /locus_tag="OO328_003801"
     CDS             <1..411
                     /locus_tag="OO328_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932191.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO328_003802"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO328_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO328_003803"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO328_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5932192.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAJTRN010000169         5532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTRN010000169 DAJTRN010000000
VERSION     DAJTRN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1097
                     /gene="celF"
                     /locus_tag="OO328_003804"
                     /gene_synonym="chbF"
     CDS             <1..1097
                     /gene="celF"
                     /locus_tag="OO328_003804"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5932193.1"
                     /translation="QLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIF
                     DIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKMFIHDVLML
                     KDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKVSGVKNIFDLPFSEG
                     LIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKV
                     KPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCKLG
                     RDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHSDRD
                     AELLAREMILAHEKWLPNFADCIAELKKAH"
     gene            1110..1868
                     /gene="chbG"
                     /locus_tag="OO328_003805"
     CDS             1110..1868
                     /gene="chbG"
                     /locus_tag="OO328_003805"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5932194.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(1915..4176)
                     /gene="katE"
                     /locus_tag="OO328_003806"
     CDS             complement(1915..4176)
                     /gene="katE"
                     /locus_tag="OO328_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5932195.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4359..4622
                     /gene="cedA"
                     /locus_tag="OO328_003807"
     CDS             4359..4622
                     /gene="cedA"
                     /locus_tag="OO328_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5932196.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            4911..5532
                     /locus_tag="OO328_003808"
                     /pseudo
     CDS             4911..5532
                     /locus_tag="OO328_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1400 a         1202 c         1408 g         1522 t
ORIGIN      
        1 cccaactgcg cgttggccaa ttaccggcgc gtgaactgga tgaacgtatt ccattaagtc
       61 atggttatct tggtcaggaa accaacggcg cgggcggttt gtttaaaggt ctgcgtacca
      121 ttccggtgat ttttgacatc gtaaaagatg tcgaagaact ttgtccgaat gcatgggtga
      181 ttaacttcac taacccggcg ggaatggtta ctgaagccgt ttatcgtcat accggattta
      241 aacgctttat cggcgtgtgt aatattccga tcggcatgaa gatgtttatt cacgatgttc
      301 tgatgctgaa agacagcgat gatttatcta tcgatttgtt tggcctcaac catatggtgt
      361 tcattaagga tgtgctggta aatggcaagt cgcgctttgc cgaattgctt gatggtgtgg
      421 catccgggca gttaaaagta tctggcgtta aaaatatttt cgatctgcca tttagcgaag
      481 gcttaattcg ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa
      541 aagagatgct ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtag
      601 tacagaaagt cgagaaacaa ctttttgagc tgtataaaaa cccggagttg aaagttaagc
      661 cgaaagaact ggaacagcgc ggtggggctt attactctga tgcagcgtgc gaagtgatca
      721 acgctatcta caacgacaag caagctgaac attacgttaa tatcccgcat catgggcata
      781 ttgataatat tccggcagac tgggcggtag aaatgacctg taagctgggg cgcgatggcg
      841 cgacgccaca tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca
      901 ttaaaggctt cgagattgct gccagcaacg ccgcacttag cggagaattt aacgatgtgt
      961 tactggcgct aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac
     1021 gcgagatgat tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc
     1081 ttaaaaaagc acattaaccg aggctgatta tggaacgctt actgattgtt aatgccgatg
     1141 attttggctt aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg
     1201 tcacatcgac gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagtc
     1261 gtgatgaacc gagtctggcc atagggatgc actttgtcct tactatgggc aagccactga
     1321 cagctatgcc ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag
     1381 aagaagatgc tttaccgctg gaagaaatta ctcaggagct tgccagtcag tatttgcgtt
     1441 tcattgagct atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt
     1501 tcccgcagat tttcccgatt gttgccaggt ttgcggctga agaggggatt gcgttgcgca
     1561 tcgaccgtca gccgctatct aacgctggtg atttaccggc taatctgcgc agttcgcagg
     1621 gattcagtag cgcgttttat ggtgaagaga ttagcgaggc actgttcctg caagtgctgg
     1681 atgacgcaag ccatcggggt gatccttcgc tggaggtgat gtgtcatccg gcgtttatcg
     1741 ataatactat ccgtcagagt gcttactgtt tcccacggtt aacggagctg gaagtgctga
     1801 catcagcgtc gttgaaatat gcaattgctg agcgtggtta tcggttgggt agttatcttg
     1861 atgtgtaagt aaggtggtaa ggtcactccc caggggacgt gaccttatcc atatttacgc
     1921 cggaattttg tcaatcttag gaatgcgtga ccacacgcgg tgttctgcca tcagcgttaa
     1981 cagttcatcc ataaaactac cgtcggtgct gtcagcttcc acaatccctt cttcaccctg
     2041 gtcagctacc ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg caatcggttt
     2101 aaggtgtttg taggcttcca tcagatagta gttggcatcg ccgttgtcag cgatatccgc
     2161 gatattgccg caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac cggcaaaggt
     2221 agcggctata ggcaacaccg taccgtcatc cgcagtcact tcacccattc gggagtagag
     2281 cagtttggca tgaacgcctt tggccttcat ggccttgaga atggccagaa ggtctgccga
     2341 tctcacttca tcattaagta aaatcgctac cacgcgacct ttcacatcac cgtcaggaat
     2401 ggcgtacaaa cttaaggatg gatccttttt cagaccgttg acgtccggag gtggggtgat
     2461 attcagctgg tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt
     2521 gagatcaata tgcgccagct ggtcaacaac gcgctcacga atatacggac gaacgacttt
     2581 gcttaactca aaactgaaac catcgacaat atggcgctgc tcgaatggcg tctgacttag
     2641 ccagaacaga cgcggatggg aataatattc gccaaacgat gggctgcgct cgcgaacttt
     2701 attgccttcc acgcgctctt ggtatgattc aaaaccgccg cgtttcggcc ccggtggtgt
     2761 ttcgcgcggc cagttatcgt tgatcgagtt cggttcataa ttcgccgggt tagtgtcgat
     2821 ccccatacga tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat
     2881 cggaatctca tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataggagaa
     2941 caaacgtccc tgcaacaggg gatcgttggt gaagtccaga ccaggaacaa tatgacctgg
     3001 atggaatgct gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat tgagcaccat
     3061 tttgccgaca cgctgaacgg gcaccagttc ttccgggatc agtttggttg gatcgagaag
     3121 atcgaagtcg aacttgaatt catcttcttc aggaatcaac tggaagccca gttcgtattc
     3181 cggaaaatcg cctgcttcaa tggcttccca caactcgcgg cggtggaagt ccgggtcacg
     3241 tccggtgagt ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca
     3301 gtggaaacgt acaaacgttg ccttcccttc ggcattaatc aggcggaagg tgtgaatacc
     3361 gaagccttcc atggtgcggt aactgcgggg gatgccgcga tccgacatcg cccacatcac
     3421 gttgtgcaga gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg
     3481 cccttgtgga attgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt
     3541 atgcgcatcc tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc
     3601 ttcagtatag aacttggtgg caaaaccacg gatatcacgc acggtatcgg cagagccagc
     3661 gccaccctga acggtagaga acctaacaaa tactggggtg attttgttcg gatctgagag
     3721 gaaatccgct ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc
     3781 tgagccgcgt gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt gggtgatttt
     3841 ctcgcgcaga ataaaatctt ccagcagcgt tggaccacgg ttaccggcac gcagtgagtt
     3901 ttgatcatcg gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt
     3961 gcgtacgtct tccagagaat taagtttttc gttacgtgta tcaggggctt tcaggctccc
     4021 aggggcggta ggttgtgcac ctggcggtgt tggttcagcc gctggacgat gagatccgtc
     4081 ctcaggtgcc agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg
     4141 gtgctgatgt gggttctttt cgttatgttg cgacattgaa ctcgtctcct taatttatta
     4201 ctgaaagggc cgctgttttg gtcaaaaaac tctctaacta tagaccacca gccagatccc
     4261 gcttcggaga ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg
     4321 gctaaatcgg ctaaactaca gtttttctaa tttttaatat gcgtttagtg aagccagtaa
     4381 tgaagaaacc gctccgccag caaaaccgcc agattattag ctatgtccca cgcacggaac
     4441 ccgcgccgcc agaacatgcg ataaagatgg attcgtttcg tgatgtctgg atgctgcgtg
     4501 gcaaatatgt tgcgtttgta ctgatgggag agtcatttct gcgctcaccg gcgtttactg
     4561 tgcctgaatc tgcccaacgt tgggcaaatc agatccgcca ggaaggggaa gtgactgagt
     4621 aaggattaca tacctcccgg ttttgccggg agttacagga cctgaaattc tgcatttcgt
     4681 tgctacattc attcaaacca cctgtaacaa aaagctgcct ttgacgagct attgctataa
     4741 ataagagatc ttttttcttt aattatttat ttattttaaa atggttaagt tttgctaatc
     4801 atgatgagtt atttttgata gttcatcctg tggcgtcgct cttaaactta tattgtaaat
     4861 gacttagatt taaataagat aataattttg ttaacaatat gaggcgggag atgttttcta
     4921 caaacttaat acagagtaat tatggtgatt taaatattaa aagcctggct ttcgactctt
     4981 ttaaagaaag gcttgagtct accatggcgg cactcacttt ctttatatca acgggtcaat
     5041 gtgactgcga tgaaatagca gagtcgaact tcaatcgtat gattgcctat atgtctaaca
     5101 ttaactatga tgcctcaaag ctaggagcgc cagcacttag ttttgatacg tatctgcagg
     5161 acaatgttaa atatagagtg ataattaata atctttatgg cagtgagttt cggataagag
     5221 atattaatga gaattttgtt gggatggatt ttacctgtga attccgaccc gaaaaaatga
     5281 cgaatttgat caatataaaa aataggttgg ttattcatta tttaaggact atatactatg
     5341 aaaaaaatta tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt
     5401 ctcctaaagt ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta
     5461 aatcttccgg gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca
     5521 cttagaaaac gt
//
LOCUS       DAJTRN010000170         5488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRN010000170 DAJTRN010000000
VERSION     DAJTRN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1630)
                     /locus_tag="OO328_003809"
     CDS             complement(<1..1630)
                     /locus_tag="OO328_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5932197.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO328_003810"
     CDS             complement(1646..2530)
                     /locus_tag="OO328_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5932198.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO328_003811"
     CDS             complement(2541..3146)
                     /locus_tag="OO328_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5932199.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5488)
                     /locus_tag="OO328_003812"
     CDS             complement(3317..>5488)
                     /locus_tag="OO328_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5932200.1"
                     /translation="DKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKL
                     IVLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDN
                     PSTIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYW
                     GLINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFE
                     GRDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQL
                     GYQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMII
                     QELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFK
                     LAEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKK
                     SPAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRK
                     NNPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLP
                     AVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPD
                     RIWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLK
                     RDPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1582 a         1365 c         1141 g         1400 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgtttatc
//
LOCUS       DAJTRN010000171         5451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTRN010000171 DAJTRN010000000
VERSION     DAJTRN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..527)
                     /locus_tag="OO328_003813"
     CDS             complement(<1..527)
                     /locus_tag="OO328_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5932201.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGI"
     gene            complement(593..1222)
                     /locus_tag="OO328_003814"
     CDS             complement(593..1222)
                     /locus_tag="OO328_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932202.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1226..1441)
                     /locus_tag="OO328_003815"
     CDS             complement(1226..1441)
                     /locus_tag="OO328_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5932203.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1734..1808)
                     /locus_tag="OO328_003816"
     tRNA            complement(1734..1808)
                     /locus_tag="OO328_003816"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1773..1775),aa:Gly,seq:tcc)
     gene            complement(1812..1887)
                     /locus_tag="OO328_003817"
     tRNA            complement(1812..1887)
                     /locus_tag="OO328_003817"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1852..1854),aa:Thr,seq:tgt)
     gene            complement(1890..1966)
                     /locus_tag="OO328_003818"
     tRNA            complement(1890..1966)
                     /locus_tag="OO328_003818"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Arg,seq:tct)
     gene            complement(1968..2044)
                     /locus_tag="OO328_003819"
     tRNA            complement(1968..2044)
                     /locus_tag="OO328_003819"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2008..2010),aa:Ile,seq:cat)
     gene            complement(2244..2624)
                     /locus_tag="OO328_003820"
     CDS             complement(2244..2624)
                     /locus_tag="OO328_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5932204.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(2617..2817)
                     /locus_tag="OO328_003821"
     CDS             complement(2617..2817)
                     /locus_tag="OO328_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5932205.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(2912..3169)
                     /locus_tag="OO328_003822"
     CDS             complement(2912..3169)
                     /locus_tag="OO328_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932206.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(3166..4557)
                     /locus_tag="OO328_003823"
     CDS             complement(3166..4557)
                     /locus_tag="OO328_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5932207.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(4554..5432)
                     /locus_tag="OO328_003824"
     CDS             complement(4554..5432)
                     /locus_tag="OO328_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5932208.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
BASE COUNT         1317 a         1534 c         1188 g         1412 t
ORIGIN      
        1 tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg tccatagcag
       61 ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg aatcgcctcg
      121 catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg gaaacactta
      181 ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg ttcggtcaga
      241 ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat agcgttaacc
      301 tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc atccaccatt
      361 gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt tgtgtgatta
      421 ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc tgcggcaatc
      481 agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga tatttatcag
      541 gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata agtcagcccc
      601 ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca cgaagaagag
      661 cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc tctgccgcca
      721 cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt tcgtctttca
      781 ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc cggacaggtt
      841 ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa ggccgatggg
      901 gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct tcctctccgg
      961 cactgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt tctgacaatg
     1021 cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg actttgtggc
     1081 agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct tcagaactca
     1141 ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga ttgaatcgct
     1201 gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc ttgtcttctc
     1261 ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga cttcccagca
     1321 caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa aaccagtagc
     1381 cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc atggatttca
     1441 tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg aaagggattt
     1501 gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa gcactctatc
     1561 caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg atcgcacgcc
     1621 cgaaggttag tatttctgca cagcaatttt gcaaaaaaag gcgatcattc ataacttaaa
     1681 cgtctttcag tcactccggg atttcccatc atcgcagact gaaagactct gactggagcg
     1741 ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag ccattatacg
     1801 atgcccgcat atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag
     1861 ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg aacctcgcac
     1921 cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg tggcccttgc
     1981 tggacttgaa ccagcgacct ggcgattatg agtcgctcgc tctgaccaac tgagctaaag
     2041 ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct gaccaccagc
     2101 gtgtttaacg tcctgtaccg tttttcaggc ataaaaaacc cgcccggagg cgggtttaag
     2161 ctgtgtggcg aagcaaccac tcttaacaga atatcccgtt ttttacgtac gtaaaatatt
     2221 ttctagaaag tcgcccctta ccatcaggga tgttcaatat atttgtccat ttctaaccgg
     2281 acacccaaca tcatcaacat tccttccacc accccctcgg ctttttgtag cttctttcct
     2341 atataaccat ctgagcatcc atgcttccgc gccagcgtca tgaatgtcat cccaaacacg
     2401 tagtagtcaa ccagcaagtc atgtagatcg ttgttgttcc ggttaaggcg ggccatacac
     2461 ccacatataa ccatcgcgtc atcgtcacag cattgcgggc gggattttac ttttgaaggg
     2521 attaatccct taaaaccggc agcaatggac gaccaggtca catcctcatg attattagcc
     2581 acccacgcgc cccagcgctc aagaacctgc tgaatatcac gcatcactgt ctttacccct
     2641 gtcccatcca cgatgaacaa tcagaacacc atcaacaatg gcgtgccctt tgccctcttt
     2701 atcttcagca tattttctta ccgtggcgcg attacagttc agcattcgtg aaacttcggt
     2761 catattacct cgtgtctgga taagcagttc cggtatcgtt tgaattttga cgctcatcaa
     2821 atactctccg gttcggtgat ttttatcccg ggctttccac cagggacata atgatcgcgc
     2881 acaagccagg gcaaaacaaa ctcatggcgc atcagcgcag cgcctcctgc accagttttt
     2941 caaactttcc gactctggtt tccagctctg ccacacagtc caccagctca tctactgctt
     3001 tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc gggtaccata tctttacgga
     3061 tagcatcttt tgttatgccc gttttttcga gttgttcagc atgacgcagc atttcctgcg
     3121 cgtgtttacg caattgttca ggggtaaaag tcattgtctg gttgttcaaa agaaacgctc
     3181 catcttactg ctgtcagttc gtttattact gtatctgcgc ggattgccgg gcttcatggg
     3241 agtggaaagc acccgtgcac tttcctggtc cacaggcaga aaatgtccgt tataaaaacg
     3301 ccggtaaatc gttcccagag aaccgttacg ttgtttcgtg atattgattt ctgcgatgcc
     3361 cctggcctgc gtatccgggt tgtacacttc atccctgtaa agcatcagaa tgatgtctgc
     3421 atccgcctct atttctccgg aatttttcag gtctgagttc atgggacgtt tattgggtct
     3481 ggactccaca ccgcgggaga gctggctcag cgcaatcaac ggaaaaccac cggattttgc
     3541 caggccttta agcccctttg agatttcacc cacggcaagg tcatgacgcc ccgtggttcg
     3601 ggtttttatc agcccgagat aatcaaccac caccagcgcc gtttccggat atttaatcag
     3661 gtggtgtttc gttgttgcgc atatctcatc aatggccagg ttcgcctggt ccaccatcca
     3721 gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa cgcgtccagt cttcatcttc
     3781 aaagtgagcc acagatttca ggcgtgatac tggcatccct ccagccgcag acaccatacg
     3841 ttcaccaatc tggatgttcg ccatctccat ggtgaacaga agcacaccat gcccctgctc
     3901 agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc cccatcgaag gacgggcggc
     3961 aatgaatacc aggtctccgg gctccatacc gcctgttttt gcgtccagtt catcaatacc
     4021 ggtcatcaac gtcctggatt tctccagccc ctgattccgg cattcaacac gctcaaccac
     4081 ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg ccctttcctg tcgacagtgt
     4141 gaccatcatg ttctgcgcat ccttcagggc atcttcagct gcttcacagg tatgcgcatc
     4201 acgtaatttc tgcagcgcct cattcagtgt tttttctgca tcgcgcagtg cggcattgcg
     4261 ccgcaacgct gcaacatagt gctccagtga agacttcacc caggttttac gcccggtatc
     4321 agtaatcacc ggggcaagtt ccggcatctc attacacaac agcacggggt caatcactcc
     4381 tgaaacacgg gcctgtctgc agatgcctgt atagatatcc tgatacgctc gtacagaaaa
     4441 aacgtccgct ggcagtgtgg ccagaatatc catcacttca ggatctgctc cgcgcagaaa
     4501 gaacgcgccg atgacagcgc cttcaaggtc atcgttacgc catactggag ttgtcatgca
     4561 gccacacctc tgatacgaga acggtaactg ggccagttaa acgacaacca gttgcgtccc
     4621 ccgtctgtga tcctgtcggc aattcgggga ctgatgaacg cccacaactc ttccggtgag
     4681 aggttactga tcagaatggt gggcaagata ctttcgtacc tggcgttgat aatttcctgc
     4741 aaaatagcca tttcagccgc gctgccaaac tgaacgccaa cttcgtcgat gatcagcaaa
     4801 tccattgacg cataacgctc aataacttca tccgctgttt tttcgctgtc attccgccag
     4861 caatttttca cagcacgggt aaggcgcatc acgtcggtga tctccacact ggccagatag
     4921 ttacggatga tgtgttttgc catagccaca gccagatgat tttttccggt accacaactg
     4981 ccggtcataa caagactggt accgttctcc agcatatctg gccagttctc cgcatagcgg
     5041 cgacaggccg caagatttct ggctgcgcca ggattaacct ccagataatt atcaaactcg
     5101 cagtcccgaa aacgcagggc aattccggcg ttatcagtca gctcttccgc cttgatggac
     5161 gacagctcca tggtcaaatc gttggcctca gcgattaagc agtcagggca gcatgaaatt
     5221 tcttctctgt cctcgccatt acgatcgctc cacaccagta tatgcgtgtg atattcgcca
     5281 tgtttttcgc aatatccgcg accttcacgc atcaggcagg aacgataagg ccatggcttt
     5341 tcgcccttct gagcaaatgc aatctctgcc cgtaactcat ccattcgcgc ctgtagtctt
     5401 ttttgttgtt cacgcaggtt aaacgtcatc atcgctgtca cctcagaatg t
//
LOCUS       DAJTRN010000172         5325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRN010000172 DAJTRN010000000
VERSION     DAJTRN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..311)
                     /locus_tag="OO328_003825"
                     /pseudo
     CDS             complement(1..311)
                     /locus_tag="OO328_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(650..1204)
                     /locus_tag="OO328_003826"
                     /pseudo
     CDS             complement(650..1204)
                     /locus_tag="OO328_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1347..1469
                     /locus_tag="OO328_003827"
                     /pseudo
     CDS             1347..1469
                     /locus_tag="OO328_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1731..2297)
                     /gene="ipgB2"
                     /locus_tag="OO328_003828"
     CDS             complement(1731..2297)
                     /gene="ipgB2"
                     /locus_tag="OO328_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5932209.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2579..2893)
                     /locus_tag="OO328_003829"
     CDS             complement(2579..2893)
                     /locus_tag="OO328_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932210.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2971..3081)
                     /locus_tag="OO328_003830"
                     /pseudo
     CDS             complement(2971..3081)
                     /locus_tag="OO328_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3405..4082)
                     /gene="ospD1"
                     /locus_tag="OO328_003831"
     CDS             complement(3405..4082)
                     /gene="ospD1"
                     /locus_tag="OO328_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5932211.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYSE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4454..4591)
                     /locus_tag="OO328_003832"
                     /pseudo
     CDS             complement(4454..4591)
                     /locus_tag="OO328_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4673..4971)
                     /locus_tag="OO328_003833"
                     /pseudo
     CDS             complement(4673..4971)
                     /locus_tag="OO328_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1456 a         1141 c         1064 g         1664 t
ORIGIN      
        1 gataagggca acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt
       61 tttaatgtcc gtatgacggt attgatgccg attttcagcg tacggccagt atcgcgaact
      121 ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa ctcccggttt acgggcctcg
      181 taacagtaag tgagctgaaa cacccggtga cagtcacggc aacggaatcg atcatgtcct
      241 tttgggttct gcccgtgacg gtaaacctga acggactggc aacgggggca atgaaagtta
      301 actgtgacca tgaaagaacc taaaagcggg tattataacc cctaattcaa ctaattggag
      361 gcattgtcat ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc
      421 ttctcatgtt tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta
      481 aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa
      541 atccggagga cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt
      601 ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg gacacaggcc taagcgaggt
      661 tctggttttc aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat
      721 tgtaatcaca ttcgatataa ttaaacactg ttgccctcat tatttcccgg ctgataaagt
      781 gttctccgtg gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat
      841 cgtagcagca gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct
      901 gataatctgc tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc
      961 tcttacgccg ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg
     1021 gtgacatgga ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca
     1081 gccagccttc atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac
     1141 tggcgtaaaa atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac
     1201 tgaccgtatt gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga
     1261 gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt tccgtaagca gccggagagt
     1321 gaaactctct ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg
     1381 tgttgttctc gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aaatggctgc
     1441 gttacgttat tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt
     1501 ggaaagttga tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt
     1561 gagcagagaa aggggagagg gatcccacat ccatgagcta ggaaaataca aaaactggct
     1621 atacaacaat tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca
     1681 attcctgggt atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggc
     1741 gattctaaat ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat
     1801 tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg caacattgct tgttgtatca
     1861 tttttaacgc ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg
     1921 cttcctgata caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgcga
     1981 taatctttct caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg
     2041 tgttctgcac aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt
     2101 tcatcatttt tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata
     2161 ctagtattta ttttggttga tgatattttt actatccttt tccccattgt acagcggata
     2221 atctcatcca cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta
     2281 aaagatgttc caagcattat aatcaccttg tcatgtaaaa tagtaacatt gtcattattt
     2341 tagctgattc aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg
     2401 gaccgtctgg aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg
     2461 cagtccctaa atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc
     2521 ctgttagttg tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct
     2581 atggtctggc atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt
     2641 tgaggctatt catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat
     2701 gcccggagcc actgttgctg ataaattcga taaaaccttg gcgtttcttg aagctatcgt
     2761 taatgcggat aatgaaacaa cgattggtga gattcgctct tttgccgacg ccctggacgc
     2821 tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct
     2881 ggcgctggag catgaagtga tttggctggg acgtagccgc tgacagcttc tataaaagta
     2941 tgatcctacc cacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc
     3001 aggactgaga cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatata
     3061 attaaacact gtatatttca tttgtggagt ctccagtctg agagggcttt atgcattccg
     3121 tattgggcac tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc
     3181 tctatcacag tgcactttac ttaatagggt gggatgtgtt cattacattt attgtgtttt
     3241 ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat
     3301 cgctcagtct agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg
     3361 gagagatcct tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt
     3421 attttgtcag aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt
     3481 tctgtaagtt ttatcccact atgatggcat atatttatat atgccatcat agcctcgata
     3541 ttccctttcc ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat
     3601 agtttaatcg tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga
     3661 atcgcagcgg aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt
     3721 cgtgctgcaa gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca
     3781 ctgtatttac gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg
     3841 ttatactcct tattattctt tgtcggaatg gatatgttct gtattttggc caattttctg
     3901 aaagaaacac cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg
     3961 gtatttgtca cgttaatgat attatttttc attcctttat tttcattagc agtattgctg
     4021 cctattatta agtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac
     4081 atttttgttc atcctttttg ctaaaaaaaa acaataacct atttttgtaa tgtaatagat
     4141 attagtctat tgctaatcta aaaaaaccat tgccacattg aatggctata ttcaccaata
     4201 aattctttcg atttcgtata ttgacatatc gtagatatat cttatcccta agattcaata
     4261 atcgagtccg tttaccattc tgtgcaagcg ccttcactca gaagtgaccg tcaaggcgat
     4321 caccaaactt gataataaag cagctcattg ccatacgcca gtccttaagg ggcgttgtcc
     4381 atttctgtga tgctgcctag atttaactgc cagctccggc ttatctacat ctaaatatgt
     4441 gagcctaata ccgctacact ttgtccagac tatgtttttc tccgggaagt gggctaaccg
     4501 tctccataaa gtgacgaact tttttcaaca gtggccatat tgaacggcac tgatgattac
     4561 gggttattgt ctcatgaaga gccggccaca tcactgacag cagtgacttc aggccttcag
     4621 caccagacag cctgaaccag ttaatccatc gcccgacaaa tttagtaatc tagggctggt
     4681 ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc acgcagagta cggatttcca
     4741 gaggttcatc tccagtagtg ataatcttcg ttaagcaacc attgcgggaa ttgctgccag
     4801 atctaagctg atttttctcg cacccaagat gatgagtcat attagcattg agtacggttt
     4861 cattttcagc atccaatcaa actgactgag atcttcagga tttttgagac ttttggctag
     4921 ttcattagct aaagcccgca acttattaga tgtcaactgt ttttcattca taaattcacc
     4981 ttcatttgat atcaaattaa atagttcaac atcagagaat tatacaaact tatgtacagg
     5041 ctcctatgat cttcgaatcc tgaagattat agtgaaaata agttttcacg ttgaaatccc
     5101 acatagttag tatatgataa ataatcatgg atgacctgat taacctcttg gggtagtgct
     5161 tttatagaag aataaatatt ctactaattt aatacatcac ctaaaatagt attgtttttt
     5221 atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc cagtgccaga
     5281 tcttacttat aataaagggg cttggcagtt tggataaact attgt
//
LOCUS       DAJTRN010000173         5253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTRN010000173 DAJTRN010000000
VERSION     DAJTRN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..572
                     /locus_tag="OO328_003834"
     CDS             <1..572
                     /locus_tag="OO328_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5932212.1"
                     /translation="WPVLSLLIIGRGCGGLGAITTGKDSQGKLPPAVYWLTLPWRIGM
                     WLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVP
                     MLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVD
                     EAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(626..1231)
                     /gene="azoR"
                     /locus_tag="OO328_003835"
     CDS             complement(626..1231)
                     /gene="azoR"
                     /locus_tag="OO328_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5932213.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1432..>5253
                     /gene="hrpA"
                     /locus_tag="OO328_003836"
     CDS             1432..>5253
                     /gene="hrpA"
                     /locus_tag="OO328_003836"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5932214.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVS"
BASE COUNT         1289 a         1304 c         1474 g         1186 t
ORIGIN      
        1 gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt ggcgcgataa
       61 caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg acattgccct
      121 ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag ccggtgagca
      181 aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca cagaatgcgg
      241 ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga ctctatttct
      301 gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag gccgtggcga
      361 tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca ttgggattat
      421 cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt aaaacagttg
      481 atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag gaagagcaca
      541 aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac tgaatatcta
      601 cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg gctgcttttg
      661 cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa
      721 atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt
      781 ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct tttttacccg
      841 ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg cgtgcaacca
      901 ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt gccgcaataa
      961 cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct
     1021 gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt tcgccatcca
     1081 gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg gagtgctttt
     1141 cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga
     1201 tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt ttgaatggac
     1261 gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc gcgcaataaa
     1321 tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat
     1381 atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt tatgacagaa
     1441 caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat gctgcgcgac
     1501 agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc tgatgcacaa
     1561 caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa agtcctgctg
     1621 cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag tcagaaaaaa
     1681 caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaaccggc
     1741 tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg tattaaaggg
     1801 ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa ccgtattgcg
     1861 gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac
     1921 gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc ggagatccag
     1981 caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca cgaacgcagc
     2041 ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta
     2101 aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat
     2161 gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt
     2221 gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga cgccgtagac
     2281 gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga gcgggaaatt
     2341 cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat cttgccgctt
     2401 tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag cggacggcgc
     2461 attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat taaatacgtc
     2521 atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg
     2581 ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg tggtcgtgtg
     2641 tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg cccggagttt
     2701 accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg
     2761 gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag
     2821 gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc
     2881 tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc acgtctggcg
     2941 cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc
     3001 gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc atcggacgaa
     3061 aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa tctgtggaat
     3121 tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc
     3181 gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag
     3241 gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg tgaaattcac
     3301 atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga taaacaggaa
     3361 tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaag
     3421 ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt
     3481 gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat taaacgcacc
     3541 tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact
     3601 gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat cgattcggcg
     3661 ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca gacgcgtcac
     3721 gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca
     3781 cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga ccagcgcatc
     3841 agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt cagccgcgaa
     3901 acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc
     3961 agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagatat
     4021 cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct gccgttactt
     4081 aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaattggtg
     4141 attgctctga ttaaatcgtt gccgaaaccg gtacggcgta attttgtgcc tgcaccaaac
     4201 tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct cgacagcctt
     4261 gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg gcactgggat
     4321 caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa caagaagcta
     4381 aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt gcaggaaacg
     4441 ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg gagttttggt
     4501 cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc gtggccggcg
     4561 ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct ggagcaaaaa
     4621 caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc gccgatcaag
     4681 tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa cccgtatggc
     4741 aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact gatcgacgcc
     4801 aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtgcgcgcc
     4861 gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg
     4921 ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact ggggctttct
     4981 gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg taacggcttc
     5041 aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact ggaaaaactg
     5101 gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt ccagcaggcg
     5161 tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga cgtgaaagag
     5221 atccgttgga tgatagaaga gctgcgcgtt agt
//
LOCUS       DAJTRN010000174         5240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTRN010000174 DAJTRN010000000
VERSION     DAJTRN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            5..913
                     /locus_tag="OO328_003837"
     CDS             5..913
                     /locus_tag="OO328_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5932215.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(959..2950)
                     /gene="tkt"
                     /locus_tag="OO328_003838"
     CDS             complement(959..2950)
                     /gene="tkt"
                     /locus_tag="OO328_003838"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5932216.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3228..3986
                     /gene="loiP"
                     /locus_tag="OO328_003839"
                     /gene_synonym="yggG"
     CDS             3228..3986
                     /gene="loiP"
                     /locus_tag="OO328_003839"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5932217.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4192..5112)
                     /gene="speB"
                     /locus_tag="OO328_003840"
     CDS             complement(4192..5112)
                     /gene="speB"
                     /locus_tag="OO328_003840"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5932218.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1261 a         1404 c         1405 g         1170 t
ORIGIN      
        1 tgtcatgaaa gcacctgttt cattaacctc atttcgccca caaaaatctc ttgctatcgc
       61 cattggcgtg cttgccgtgg ttgtgctgcc atttctctcg tattacaccg ttaatgaagg
      121 tgaacgcggc attttgctgc gctacggaaa aatcgtaaaa gttgccgaac cggggctggg
      181 ttttaagatc ccttttatgg aaagcgtgga gaaaatttcc acgcgtaatc aggctgtggt
      241 ctatcaggga ttacaggcct acagccgcga ccagcaaccg gcacaaatga cagtatcagt
      301 cagcttccac attaaacctt cagaagcagg cgcggtttat accacctaca acactattga
      361 agcattaaaa gatcgtctaa ttgtccgtca gttgccaact cagctggaga acattttcgg
      421 ccaatatacc gccatcagcg ccgtacagga tcgtacaaaa ttggttcagg atctgcaaaa
      481 tgccatgcgc aaagccgttg ttgggccagt ggtaattgac ggggtccaga ttgaaaatat
      541 tgatttttct gacgcttatg aaaagtcgat tgaaaaccga atgaaagcag aagtcgccat
      601 tgccacgcgt aagcaaaatc tggaaacaga gaaaatccag gcgcaaatag cggtaactca
      661 ggcccaggca gaagcagaca gtaaactggc ggcagccaaa gcagaagcgg aaactattcg
      721 cgtaagaggc gcggcagagg ctgaaactat tcgtttaaaa agtgcggctg aagcagaagc
      781 aatccgcctg cgcggtgaag ctctgcggga taatcccgga cttgtagccc tcaccactgc
      841 cgaacgttgg gacggtaaac tgcccgatac catgatcccc ggcagcacag ttccgtttat
      901 ctctactaag taaaaaaagg gccgctttcg cggtccttct cacaaaacgt cttactgatt
      961 acagcagcgc ttttgctttc gcaacaacgt tatcaacagt gaagccgaac tcttcaaaca
     1021 gcagctctgc cggagcagat tcaccgaagg tggtcatgcc gacgatagcg ccgttcaggc
     1081 caacatactt gtaccagtag tcagcaatac ccgcttctac cgcaacgcgt gcagtaaccg
     1141 ctttcggcag tacggattca cggtaagccg catcctgctt gtcaaatgcg tcggtagacg
     1201 gcatggacac cacgcgcgct ttcacgcctt cggcagtcag tttttcgtag gcagcaaccg
     1261 ccagttcaac ttcagaaccg gtggcgatga agatcagttc cggctgacca gcacagtctt
     1321 tcagcacata accaccgcgc gcgatgtttg ccagctgctc ttcagtacgt tcctgctgcg
     1381 ccaggttctg acgggagagg ataagcgcag tcgggccgtc ctgacgctca acgccgtatt
     1441 tccacgcgac cgcggattca acctggtcac acggacgcca tgtagacatg ttcggggtca
     1501 cgcgcagaga agcgacctgc tctaccggct ggtgagtcgg gccatcttcg cccagaccga
     1561 tggagtcgtg ggtgtaaacc atcacctggc gctgtttcat cagcgcagcc atacgtacgg
     1621 cgttacgtgc gtattccacg aacatcagga aggtggaggt gtacggcagg aagccaccgt
     1681 gcagggagat accgttagca atcgcggtca taccgaactc gcgaacaccg tagtggatgt
     1741 agttacctgc agcatcttcg ttgattgctt tagaaccaga ccacagggtc aggttagacg
     1801 gtgccaggtc agcagagccg ccgaggaatt caggcaacag cgggccgaac gcttcgatag
     1861 cattctgcga cgctttacgg ctggcgattt tcgccggatt agcctgcagt ttagcgataa
     1921 actctttcgc tttggcgtcg aagtcagacg gcatttcgcc tttcatacgg cgggtaaatt
     1981 cagccgcttc ctgcggataa gctttcgcgt aagccgcaaa cttctcattc catgcagatt
     2041 ctttcgcctg gcctgcttct ttcgcatccc actgagcata gatttcagac gggatttcga
     2101 acggtgcata tttccagccc agctgttcgc gggtcagggc aatttcagcg tcgcccagcg
     2161 gcgcaccgtg ggagtcgtga gtaccggctt tgttcgggga accgaaaccg atgatggttt
     2221 tgcacatcag cagggacggt ttgtcagtca ctgcgcgcgc ttcttcaact gcgcgtttga
     2281 tggatgccgc gtcatgaccg tcgatgtcgc gaataacgtg ccagccgtaa gcttcgaaac
     2341 gcattgctgt gtcgtcggtg aaccagcctt caacgtgacc atcgatagaa ataccgttgt
     2401 catcgtagaa cgcaatcagt ttacccagct tcagcgtacc cgccagagag caaacttcgt
     2461 gggagatgcc ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag tggtcgacaa
     2521 tgtcgtggcc cggacggtta aactgcgccg ccagcgtttt ttctgcaatc gccataccga
     2581 ctgcgttggc aataccctga cccagcggac cggtggtggt ttccacacca gcggtataac
     2641 ctacttccgg atggcccgga gttttagagt gcagctgacg gaagtttttc agttcttcca
     2701 tcggcagatc gtaaccggtg aggtgcagca ggctgtagat cagcatggag ccgtggccgt
     2761 tggacagcac gaagcggtca cggtcagccc aggacggatt ctgcgggttg tgtttcagga
     2821 aatcacgcca caggacttcg gcaatgtcag ccatacccat aggggcaccc gggtgaccgg
     2881 atttggcttt ctgtactgcg tccatgctca gcgcacgaat agcattggca agctctttac
     2941 gtgaggacat tttgactcca gatcggatga tgaagggcac gcccttaacg acttgacgac
     3001 agcgcgtttt gggctacgcc ggaaaatttg ccaacaattt accgcaagcc gcgcgtcatg
     3061 tacatggaac atccttttgc cgcttcagaa atctctggat catgctcgca tgttgcgcaa
     3121 tctgctcgcc cgtccgctgc gcttttcctt atactgagac tgagcgtcga ttcacctgca
     3181 aacggcgcat ttttagaata atcctgacct tgtgcggaag agaaaacatg aaaattcgcg
     3241 ccttattggt agcaatgagc gtggcaacgg tactgaccgg ttgccagaat atggactcca
     3301 acggactgct ctcatcagga gcggaagctt ttcaggctta cagtttgagt gatgcgcagg
     3361 tgaaaaccct gagcgatcag gcatgtcagg agatggacag caaggcgacg attgcgccag
     3421 ccaatagcga atacgctaaa cgtctgacaa ctattgccaa tgcgctaggc aacaatatca
     3481 acggtcagcc ggtgaattac aaagtgtata tggcgaagga tgtgaacgcc tttgcaatgg
     3541 ctaacggctg tatccgcgtc tatagcgggc tgatggatat gatgacagat aacgaagtcg
     3601 aagcggtgat cggtcacgag atggggcacg tggcgttagg ccatgtgaag aaagggatgc
     3661 aggtggcgct tggtacaaat gccgtgcgag tagctgcggc ctctgcgggc gggattgtcg
     3721 gcagtttatc tcaatcacaa cttggtgatc tgggcgagaa attagtcaat tcgcaattct
     3781 cccagcgcca ggaagcagaa gccgatgatt attcttacga tcttctgcac caacgcggca
     3841 tcagcccggc aggtcttgcc accagctttg aaaaactggc gaagctggaa gaaggtcgtc
     3901 aaagctcaat gtttgacgac catcctgcat ccgccgaacg tgcccagcat attcgcgatc
     3961 gcatcagcgc ggatgggatt aagtaaagcc tagtggtgtc gaacgtaggt cagataaggc
     4021 gttcacgccg catctgacat taatggcaca tttgttacct tgtgcgcatt gccggatgcg
     4081 atgctggcgc atcttatccg gcctacgggt gccgaacgta ggtcggataa ggcgttcacg
     4141 ccgcatccga cattaatggc acgttttacc cgtgcgcatc gcatctggtg cttactcgcc
     4201 ctttttcgcc gcctgaatat acagcatctc cagcgccagc gtcgccgcag ccagagcggt
     4261 gatttccgac tgatcgtatg ccggagccac ttccactacg tccatcccaa cgatgttgag
     4321 atctttcagg ccgcgtacca gtttaatggc gcgatcggag gtcaggccgc caatcactgg
     4381 cgtaccggtt cccggcgcga aagcaggatc caggcagtcg atatcaaaag tcaggtaaac
     4441 cggcatatca cccacaatct gtttcacctg ggcgataacg tcatccacgc tgcgatcgtt
     4501 cacctggcag gcgtccagca cggtaaagcc gttgtcttta tcaaactcgg tacgaatacc
     4561 aatctgcacg gaatgattcg ggtcgatcag accttctttc ggcgcggtat agaacatagt
     4621 gccgtggtca aattcacaac cgttcgcata ggtatcggtg tgggcgtcaa agtgtaccag
     4681 cgccattttg ccgaaatgct tcgcatgagc acgcagcagc ggcagcgtaa caaagtggtc
     4741 accaccgaag gagagcatac gcttaccggc agccagcagc ttctcggcgt gcgcctgcag
     4801 cttttcgctc atctcacggg catcgccaaa ggcatatacc agatcgccgc agtccacgac
     4861 gttcagacgc tcacgcatgt cgaaattcca cgggaagcgg ttgtgttccc aggccagatt
     4921 cgtcgaaacc tgacggatcg ctgccggacc gtggcgacca cccgcacgac cagaagtggc
     4981 catatcgaac ggcacgccag taatcaccca gtctgcatcg ctgtcatacg gctggaagtt
     5041 catcggcagg cgtaaaaaac caaaggcatt ggaaaccagt gagttatcgt attgatgacc
     5101 taaggtgctc atggatggac tcctattaca aagtcgatat aaaaaaaccc ttccgcgtcg
     5161 ttaggcccga cgaggaaggg ttggatttgc gaaacacaag tttgtgctaa ttatcgcctt
     5221 aaatgggcga ggcatcaagc
//
LOCUS       DAJTRN010000175         5183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTRN010000175 DAJTRN010000000
VERSION     DAJTRN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..938)
                     /locus_tag="OO328_003841"
     CDS             complement(<1..938)
                     /locus_tag="OO328_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5932219.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDG"
     gene            complement(950..1439)
                     /locus_tag="OO328_003842"
                     /pseudo
     CDS             complement(950..1439)
                     /locus_tag="OO328_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1443..2663)
                     /locus_tag="OO328_003843"
     CDS             complement(1443..2663)
                     /locus_tag="OO328_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5932220.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2667..3410)
                     /locus_tag="OO328_003844"
     CDS             complement(2667..3410)
                     /locus_tag="OO328_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5932221.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3301..4767)
                     /locus_tag="OO328_003845"
     CDS             complement(3301..4767)
                     /locus_tag="OO328_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5932222.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTAMGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4767..5000)
                     /locus_tag="OO328_003846"
     CDS             complement(4767..5000)
                     /locus_tag="OO328_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5932223.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
BASE COUNT         1102 a         1461 c         1306 g         1314 t
ORIGIN      
        1 atgccatcaa cgtacgaaat ggtttctttg tacactggct cgactgcacc cagcttgccg
       61 tagtaagtga cgatctgata cagaccgcga tactgcaccg gcacgctctg aagcggaacc
      121 agcgggtagc ggacgtattt tttatcgttg gtgtacgcaa ctatgcgatc cttattcccc
      181 acaccacggc ctttcagcca tttaaccgcg cggatattca gcggaacacc gttctggtga
      241 tagctgatgg tgttggtctg aaggttcgtc aacagggact ggttacctgc agatgaaacg
      301 atgatgctgg acaacagagc aaactgctca ggcgggatca gcaaatcacg cggaaccaca
      361 gagtaaccag aagcggccca cgcatcagac agcacctggt taatgcttgc gcggatttcg
      421 tccggtgttg aggttgccca cgttttggca gcgttgttga caggcacgcc gtccagggta
      481 acaaggcctt tcaggtttaa tgcggaatcg ccaacatata cctgttcatc gttatccatc
      541 tgccatttca gttgcatacc gtcatacttc tgcgtatcaa tcgggcggcc gacctgctga
      601 gcagcctgca attctatgac cgtccagcca agttccatcc cccacaggtt cagcgggtta
      661 ccggatttgc cgatatccac gttcacgcca gcaatagcgg ttgagtcttt gcctacccag
      721 ttttttccat tcggatttgc gccagtaccc gcagcggcga agctggtatt cgtccagctg
      781 gaaatgtcat ctgcgatgga ggcgtcttca cgcagttgaa tatcgcggct ccaggtgtac
      841 cccaccagtg gcaggttcag cgtctggtcg agtctctcca gctccccgat gagaaaggca
      901 ccagagctgt caacggttgc ctgatcaaaa gtaatcattc gtctgttcct taaatcttcc
      961 aggaaatttc tgcattgccg tcagcatcac cggcacctgt gaattcagcg ttggtcagca
     1021 ccacgttttt gccactgact gacgtggaca tgaatccacc cagaggcact ttgatggatt
     1081 catcagtgga gatcgccttt tttgatagtg ctggcatcaa aatcagaacc gagattaacg
     1141 gtcacgtagc cacgcttcat ggcgtcgccc gggaagttct tgcctgttcc cacctggcga
     1201 accatgtccg gctgcgacgt ggtcggataa gggcgcacgt agatcccctt caccttgtct
     1261 gcggtatcac catctgccag cggcacgaaa aaaccgtcat catcgtattt accagccagg
     1321 ccataggcag cgaaggcgtt atcggattta aggaccaccg gttcgacggt taagtcctgc
     1381 gggcgagaga cagccccggc aatgccaaca ggcatccggt acagaaatac attattcatt
     1441 ttttaccctt tacggtttgc ccagaattca gcgttttgtt tgttcaggga agcgatactg
     1501 gtcatgccca tgtttaggcg ctgtgcatcg ccggtggtgg cgcgggtgtt tcgccctttg
     1561 gcaacctcag acacggcatt aaacgccatg tcgaccgatt gtttcggtaa tttgcggata
     1621 tccgcatcac cgactatctg gcgaaccagc gttttgtcag cagaagccag aacctcgcgt
     1681 ttgaacgcgg tcggtttcat cttacggctc agatcgatac ccggaacgat aacttcggca
     1741 cgccaggctg agtcaccagt aatcgtggtt tcctcttcat cgtcctcgcc gtcaccggtc
     1801 ggattatcgt caggcttatt atcgttatcg cccgtggcat ttctttccag cttagccagc
     1861 agggctttca gtaatgtttt gaggtcatca tcactgtcgc cggttggatc tccgcccatc
     1921 tctggtgctt tgtccggtag tggttgctgc ggggacaggt tgatgttgag attaacgccc
     1981 tgcggcaaat ccccctcatc tcctgtaacc gatgcgggag ccgactccac cagttcgttc
     2041 atggtgtcgg catctcctgt cttgatggcc gcacgcatgc ggttccacca gtttttcttt
     2101 tgatttgcca ttgtgtctct gtctccaatt gcacaacgat ttccggctct gcctttaggg
     2161 acaagagcca catggtttcc ggtaatatcg acctgctcgg ctttacctgg ctcggtctgc
     2221 tcgtactccg cgtcatagcc gcacgacact tcgcgcaggc catcttcgat aagctgaatg
     2281 gcgctttcgt ctttgacgat aaggtcagcc agcatcaaat cagactgctc acccgtcccg
     2341 cgccggacat tctggaggtg cccgacagca agcactttcc agttctcggg atttaccagc
     2401 cgcacattcc cgttttcatc ttcaggatgc aggatcgtga tgctcatccc ttcgaatgag
     2461 gcaagcgtgg ccggatggaa tacctgctca ggagaacgcg tgacgactat ttcaacgaac
     2521 ttatcgggtt tcagttttgg caggtcatca gcaccataga gctgcttacc tgttcgtcct
     2581 atcggcacgt ctttgcacag caacgagccg tcagccaggc ggtatcgggt ttccccgagc
     2641 ctggtctcaa aaaagtattt catgggttat gctgcccttt tcattgcgaa tggaacgcga
     2701 acaaacacga tctctttata gcagcggcag tttggcagct cgcccgcgtg gccggtcatg
     2761 ccgtccaaag ttggaggtga atcccagcgg acgaatttac ctttcatgtg atcgtgggaa
     2821 tctcggacat cgccatcatc ggctgtacgc caaatataac catccgagcc aatagctatc
     2881 gctcgggcca tatccagcgc gcccgttgct cttcccagtt ccgttcgggc aatcagatcg
     2941 gccctagact tagcaacatc gccggtagat gcaatctctt tagcaaactg ctccgcccga
     3001 ccaccggtga caacagcctc tatcgctttg ttctggatgt cgtacaccct gtctgcggcc
     3061 tcgagaggca gagatttgat gtacttgacc tgttcggcga tgatggattg catcacctgg
     3121 cccacaggag cgctttccac aagattgcgg agctcgcgac tgatgttctt gctgtgttgc
     3181 cgccaaactt tctcgttctg ccgggttagg tccgcagtaa agttttccgc gacctttgtc
     3241 gcccaggggg tgatgatttc actgtaacgc tccagcgccg caataatttc cgtgatactg
     3301 tcatttgaac catcgtagcg accatttacg atgtctccga ccgccctcgc tatcctgcgt
     3361 aggctggttc gataccggat ttccgcctga cggctcctgc ggttcgtcat caggttcgcc
     3421 gatgccgggc ggcgcttcat cttcggcatt ctcgatgtcc tcgttggtaa tggatgcccc
     3481 tatgccggtt acgtcagaat tttctcgcaa atcagtcata gcggctttca gtgtcatcag
     3541 accatcaccc atcgccgtac tgattgcgtt ggtggtgttt aacgccaccg ttgagcgatc
     3601 gacatcagac atttgccaga gcgggttaaa ctcaaacgtg aaatcgtccg gcagcggctt
     3661 gccaagttcc gaacgatgca tgatgtccag tatccgccgc accggaagac gtaaacgcct
     3721 ctcctgcaac gaactgatgc gatcgtaata gttggcaagg tctgcatcac cggtagaaaa
     3781 tcctttcggg gactgtccga acaaccgcac cagtgggata ccaacagcgc cactaatctg
     3841 ttctgcaaac tgcgaaagga tgtcatccag accactgaag ctgtactgat gggtttcaaa
     3901 cttatcccgc gagtccatga gcgtcatgcc ttcattactc tggaactgtc gaatcaggtc
     3961 gatattcttc agcaacgctt catacgcagg accaccaagt gcgataagct cgcgtagctt
     4021 ctccacgctg taggtacgca aatgcgcctt gtagaccagc tgcgccgcgc cgacagtagc
     4081 gctgtcgaac gcggtaagac gatcccagat acgctctaca accgacattc cccattcgtt
     4141 ctcggtcatc ttctgctgaa atggcaacgt gacgccatca aagcgaatca ggcgactgtg
     4201 atgaatgcgc caggcaggaa ttcccgttgc tgtggtcacc acatcgtaaa actcaggttt
     4261 acccaggtcc ggccccatat ctttaatgcg gcgggtcagt gccgggtcga ccatccagcg
     4321 gtcgagcggg agaatcccct taaacttgcc cttaccgatg gtttcgggtc gcagcggggt
     4381 cattggtgcc tgcccctcaa tcatgatgaa acccaccgcg ccgccgtaga ggcgcgacca
     4441 tttcagcacg tcattcagcg catcccagat ttgcaactca tccagttgtg attcgagaat
     4501 gccacgatct tttgcatcaa tttccgaagt gatgcgaatg cctttgcggg tcatatcatc
     4561 cgggatagca tcgactgctt cgccgatgat ccaggatgaa cgataggacc attccaccag
     4621 catgcggtta cgactggtga aattagcccg gtaggtggat gctgagtgct ggttaggtgt
     4681 ctgcatccct actcgggcaa taaaattctc ataaccatca gctgtggcct gcacagttcg
     4741 ccgcagggct tgtttgtttc gtgccatcag gcctgtctcc ccagctgttc ccagatatcc
     4801 agcgatgtat cgattggcgc gaaggccata atgaatgcat cagcaacgtt tggtgacggt
     4861 atctcgcgtt ttgcgaggtc ttttttactt tcgaccatca cacgtccgtt acggtcgaaa
     4921 tcacgatgag gtgttgtcag ttccagtttc agcttttcaa gcaacggaca acgagaatct
     4981 atgctgatca gctcatccac aggatactgt tctccgttgt taatggcgtt aaacgtattt
     5041 ctgaaacggt cagccaccat gcctgagcct taagatttgc gaaaaagtct ttgttgggga
     5101 tgccgttgta ctcgtcatct ggctcatgca caccagcacc ggcgttaaac ctctggtaat
     5161 tcacacgtcg cgcgtatgcg ttc
//
LOCUS       DAJTRN010000176         5166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRN010000176 DAJTRN010000000
VERSION     DAJTRN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..762
                     /locus_tag="OO328_003847"
     CDS             <1..762
                     /locus_tag="OO328_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5932224.1"
                     /translation="GSITLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYK
                     GSLYWNNVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIH
                     MYKIATLGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYC
                     SSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVN
                     KSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1096..3375
                     /gene="ydeP"
                     /locus_tag="OO328_003848"
     CDS             1096..3375
                     /gene="ydeP"
                     /locus_tag="OO328_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5932225.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3622..3825
                     /gene="safA"
                     /locus_tag="OO328_003849"
     CDS             3622..3825
                     /gene="safA"
                     /locus_tag="OO328_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5932226.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3893..4654
                     /gene="ydeO"
                     /locus_tag="OO328_003850"
     CDS             3893..4654
                     /gene="ydeO"
                     /locus_tag="OO328_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5932227.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5056..>5166
                     /locus_tag="OO328_003851"
     CDS             5056..>5166
                     /locus_tag="OO328_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5932228.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKA"
BASE COUNT         1472 a         1099 c         1158 g         1437 t
ORIGIN      
        1 ggcagcatta ccctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc
       61 ggctggtacg acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta
      121 cagtcatata aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca
      181 aataccaacg tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat
      241 attaccccag ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt
      301 attcatatgt ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat
      361 attatttcaa ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat
      421 gtcaccgtgg atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc
      481 tcaagcgaac aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa
      541 gtgtttgcta acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg
      601 cgtaatggaa aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc
      661 aaagtacctc tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga
      721 accgtccagt cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag
      781 tctatgcgct ccattcacga aaattgcgcg gataattatt ttgtgaaggc tattatccta
      841 cacctgtaag aaaatccgcg cacaattgaa gccagagtct tattttttat attggaaaat
      901 atgttaattt aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta
      961 acgataatag attaaaaata gaaagagagt agtcccttac tcttatatat ccatgttggc
     1021 gataatccct ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc
     1081 acacaggata agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg
     1141 ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt
     1201 attgccatgt ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca
     1261 gatcctaaga acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcttgg
     1321 gaagtcacgg ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta
     1381 cttacctggg gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat
     1441 gatgccgtca gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc
     1501 gcacgccttc aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact
     1561 tccaatgaag ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt
     1621 cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt
     1681 gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt
     1741 gggcataacc ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa
     1801 cggggagcga aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc
     1861 gcaccgcaaa acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac
     1921 tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt
     1981 gagcgcgatg atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt
     2041 caaacgcata ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa
     2101 gatatcgaac gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat
     2161 gccgctgccg aacgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt
     2221 acccagaacg tgcagcaact ggtcaatttg ctgttgatga aaggtaacat tggcaagcct
     2281 ggtgcgggta tctgcccact acgtggtcac tctaatgtac agggcgaccg aaccgtcggt
     2341 atcaccgaga aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcccc
     2401 ccacctcatg cacctggaca tgctgcaatt gccagcatgc aagcaatatg tacggggcag
     2461 gctcgagcat tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg
     2521 agcgctgtac cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc
     2581 tctcatctgc tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt
     2641 gatatgcaaa aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat
     2701 gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc
     2761 gcgggaatcg cgcaggcagc actaccccag agcgtggtag cctgggagta tctggtggaa
     2821 gattatgatc gcattcgcaa tgacattgaa gctgtgctgc cagagttcgc cgactataac
     2881 cagcgcatcc gtcatcccgg tggttttcac ctgataaatg cagctgctga aaggcgctgg
     2941 atgacgccgt caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct
     3001 tcagcgttta acagtaagct ggtcatggcg acagtacgca gccacgatca gtacaacacg
     3061 acgatttatg gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt
     3121 atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt
     3181 gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt
     3241 taccctatgg ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaaca
     3301 cttgataacc acgatccatt aagtggtatt cctggctata aaagtattcc ggttgaatta
     3361 gaaccatcaa attaatgcct cttctcattt cttctgctgt catccgcaca gcagaagaat
     3421 tcctcattga ctattattgc gcaatttgct cacatggatt aaattaaact acatactata
     3481 agatataaac ttctgcctac agctgtaaga aactccgctc agtactgaag caccagtcct
     3541 atttcttctt ttctccagcc tgttatatta agcatactga ttaacgactt ttaacgttat
     3601 ccgctaaata aacatatttg aatgcatgcg accacagtga aaaacaaaat cacgcaaaga
     3661 gataactata aagaaatcat gtctgtaatt gtggttgtct tattactgac acttacgttg
     3721 atagccattt tttcggcaat tgatcagctg agtatttcag aaatgggtcg cattgcaaga
     3781 gatcttacac atttcattat caatagtttg caagactgga aataaacagc aaattataaa
     3841 tgtgaaatgt ttaaaaggta tcgataaaac tttattgttt taaggagata aaatgtcgct
     3901 cgtttgttct gttatattta ttcatcatgc tttcaacgct aacattttag ataaagatta
     3961 cgccttctcc gacggcgaga tcctgatggt agataacgct gttcgtacac attttgaacc
     4021 ttatgagcgg cattttaaag aaatcggatt taatgaaaat accattaaaa aatatctaca
     4081 atgcactaac atccagacag tgacggtgcc tgttcctgcg aagtttttac gtgcttcaaa
     4141 tgtaccgact ggattgctta atgaaatgat tgcttatctc aactcggaag aacgcaatca
     4201 tcataatttt tcagaacttt tgcttttttc ttgcctgtct atttttgccg catgcaaagg
     4261 tttcattaca ctattaacta acggtgtgct atccgtttcc gggaaagtga gaaatattgt
     4321 caacatgaag ctggcgcacc catggaagct gaaagatatt tgtgactgcc tgtacatcag
     4381 tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg acattctcac agattctatt
     4441 agatgccaga atgcagcacg caaaaaattt gatacgcgta gaaggttcag tcaataaaat
     4501 tgccgaacaa tgtggttatg ccagtacatc ttattttatt tatgcgttcc gcaaacattt
     4561 cggcaacagt ccgaagagag tttctaagga gtaccgttgt caaaggcaca cgggtatgaa
     4621 tacgggcaac acgatgagtg ctttagctat ttgattattt gctaacgagt agtcaaccac
     4681 gcacgctacg taagaattat atggggcagc cattccctgc cccgcgttgt ttttaggcga
     4741 tatatttatt gaaataaata agtgacatcc atcacatatt tatgcacttg cataacctgt
     4801 tgcatgatta tttatgatct caattcggca ttttgtcagt aaaatgcaat aatttattaa
     4861 atatcaataa attagttgtt tatcggcgag aaattactta atagaacaga aagtaatgtc
     4921 aacgctttat ggactgttat ttcccttttt ttagctaaat ctgctatctc tttatgtgac
     4981 taacttcact tacatccact tatttctctt cgtaaaatta cttcggaatt aagtacaata
     5041 agaagaggaa catttatgaa gtctgcatta aagaaaagtg tcgtaagtac ctcgatatct
     5101 ttgatactgg catctggtat ggctgcattt gctgctcatg cggcagatga tgtaaagctg
     5161 aaagca
//
LOCUS       DAJTRN010000177         5151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRN010000177 DAJTRN010000000
VERSION     DAJTRN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            190..447
                     /locus_tag="OO328_003852"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO328_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO328_003853"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO328_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5932229.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO328_003854"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO328_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5932230.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO328_003855"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO328_003855"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5932231.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO328_003856"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO328_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO328_003857"
     CDS             complement(3605..4420)
                     /locus_tag="OO328_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5932232.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTCYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="OO328_003858"
     CDS             4517..>5151
                     /locus_tag="OO328_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5932233.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcacgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAJTRN010000178         5086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRN010000178 DAJTRN010000000
VERSION     DAJTRN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..224
                     /locus_tag="OO328_003859"
     CDS             <1..224
                     /locus_tag="OO328_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5932234.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO328_003860"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO328_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5932235.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO328_003861"
     CDS             518..1108
                     /locus_tag="OO328_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5932236.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO328_003862"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO328_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5932237.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO328_003863"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO328_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5932238.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO328_003864"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO328_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5932239.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OO328_003865"
     CDS             3457..3648
                     /locus_tag="OO328_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932240.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3878..5065)
                     /locus_tag="OO328_003866"
     CDS             complement(3878..5065)
                     /locus_tag="OO328_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5932241.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNTTHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
BASE COUNT         1322 a         1324 c         1254 g         1186 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt tttgctccgt
     3841 ttccctcaaa atggacgcaa cttcccctct gcggctctca gccgcaccac cgcatccggg
     3901 ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc agccccgtaa
     3961 aacgcatctg actcccgcac agcacgcact tcagcgggtc aaccttcagt aacctctgat
     4021 acatccctct ccaggtgatt tgcatcgccg tttttctcac tgtctccgtt atgatgtaca
     4081 ccacttcttc cagtaaccgc cgtttcgccg gactcaaaaa accgtagtac ctcaccatac
     4141 ggaacccctt atccgccaca tgccaggaga acctttccat gaactcatct ccactcatca
     4201 acaggtattc ttcccgtttt gttcggtgac tgttgtaacg cagaccgatt tcatcctgac
     4261 cggcataatg ctccagacga ctcatcggca ccggtggctt tttcaggtaa gagccaaagt
     4321 acaccgccac atgggtggta ttatccatta cccgggatac gttgacattc cagccacggc
     4381 ggtaatgcgt gtccaggaag cgattccatt cccgtttact gcttccttct gctgccagcg
     4441 catccggcat caccaggtca gggtatttcc gtgacagcaa ccgtgttatc cggtagcgcc
     4501 acatgctcat caccttacgg gcgtaaaaat gaagattttt ccaggtgtgg cccgacgtca
     4561 caccaccggc agttgtcgat aaatggatat gcggatgcca ctgctggtca cgcccccatg
     4621 tgtggatcac cgtgaatatc cccggctcca catctgcctg atggcagatt tccagtatca
     4681 catccgctgc aatgcggctc atctctgcca gtaaccaccg gttgtggaac accagggacc
     4741 agtactggca gggaagtgtg aacacaatat gctgccacgg gcagtagggg accaggctca
     4801 gcagatactg tatccactgt gcgccagcct tcaccccgca gtgcgggcag gagcggcttt
     4861 tacaccggaa gcagaccttt tttgtatggc aacagtccgg tgatgaacaa caccactgcg
     4921 tgtagcccat cagtgtggtc ccgcaggcca tgatttttgt cactgattca attaccaccg
     4981 gacggactgc cccttccggt tgtttctcca gccagttaag ccagcggttt ccctgctgaa
     5041 aaatatcggc aaaacggggg agcatcagaa gggcggggcg actccg
//
LOCUS       DAJTRN010000179         5037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRN010000179 DAJTRN010000000
VERSION     DAJTRN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..259
                     /locus_tag="OO328_003867"
     CDS             <1..259
                     /locus_tag="OO328_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5932242.1"
                     /translation="LVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAVLVASQL
                     IGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(260..493)
                     /locus_tag="OO328_003868"
     CDS             complement(260..493)
                     /locus_tag="OO328_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5932243.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(579..752)
                     /gene="ortT"
                     /locus_tag="OO328_003869"
     CDS             complement(579..752)
                     /gene="ortT"
                     /locus_tag="OO328_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5932244.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            947..1042
                     /gene="yncL"
                     /locus_tag="OO328_003870"
     CDS             947..1042
                     /gene="yncL"
                     /locus_tag="OO328_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5932245.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1444..2253)
                     /locus_tag="OO328_003871"
     CDS             complement(1444..2253)
                     /locus_tag="OO328_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5932246.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2463..3887)
                     /gene="patD"
                     /locus_tag="OO328_003872"
     CDS             complement(2463..3887)
                     /gene="patD"
                     /locus_tag="OO328_003872"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5932247.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3909..4703)
                     /locus_tag="OO328_003873"
     CDS             complement(3909..4703)
                     /locus_tag="OO328_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5932248.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4693..>5037)
                     /locus_tag="OO328_003874"
     CDS             complement(4693..>5037)
                     /locus_tag="OO328_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5932249.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1356 a         1298 c         1075 g         1308 t
ORIGIN      
        1 actggtgtcc agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt
       61 cttcgccagt atcagcggat atcagaatgt gggagcggcg acaactattg cggtactggt
      121 cgccagccag ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact
      181 acgggcgttg ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt
      241 ggccagacgc tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg
      301 cttccatatc ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct
      361 gctgggtgta gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt
      421 cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat
      481 ccagatgcga catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat
      541 ataagtctgc gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc
      601 gccaccggga aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg
      661 cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca
      721 taaaaaacaa gcatatgttg atagagaaac atgtacgggc gtccagccat atgaataaaa
      781 cgcgcgcaaa tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa
      841 aatcatcaaa cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc
      901 ttaacccgta ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag
      961 tagaactgta gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat
     1021 gagatttggc tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa
     1081 tcaactctct gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt
     1141 aatatttact tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg
     1201 tcgtctatac aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac
     1261 gaatacttta tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat
     1321 ataaaaagat ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacgct
     1381 gccccattga aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga
     1441 taattatagg cctctgaata cctcagaaag attgaatttt ttatagtccc tctcttgaat
     1501 atttccattt gatatcaatc gattcatctc ttcaacaaaa taatttatat catcacactt
     1561 attattatcc ggaagatctg agtagttata attccaccat tttattctca acagttcatc
     1621 aataacattt gaatcaaacc taaatctgat tatttttgca ggaactcctg caactatggc
     1681 atatgggggc acatctttgg taacaacgga attggcagca ataacagcac catcacctat
     1741 tgcgatgcca cctttcaata caacatcatt tcctatccag acatcatttc ctataatcgg
     1801 attttcaggt ttggtaatga atggtttcaa tgtccagcta cgttcaaatt cactcatagc
     1861 aaacttatca aattcaccgt tataagtcat catgtgagta gtaaaacgat tcaaaggatg
     1921 atttccaccc attattttta catttgatgc aatactacaa aatcgaccta tacgtatatt
     1981 ccctgaaaaa tggcaggtcg gaaatgaaaa agcgccgaca ctggagaaat ttttcacagg
     2041 cattgttgaa tattcttcag caataacatt atcttcaatt gttatcttgt caccttttga
     2101 gaaaagaaat aaattccttt ctttatacga cttcttcccc ttatgattta aaaagatttt
     2161 attatccata caaaactcaa agtgtttttt cgtccatctg aaggtaaggg ttttattttt
     2221 atcttccgtg aatttgtaca attgaacttt cattgttaat acttctactt ataaagtatt
     2281 agtttaataa cgatactcta aagccttatg ctaatcaact gtgacaggat gaaaaatcgc
     2341 catcaaacat ttagcctaat ttttacgcca gttattaatg taatcattct aatacttaac
     2401 cgccattaaa ctcagaacct aaaacgatag tggccgtcct ataacagcca ctaacatcta
     2461 acttaatgtt taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac
     2521 atatccttgc cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg
     2581 aaatgggtat tgacccaggt acaaccatat tgcagccgtg cgctgacgcg atgcgccctg
     2641 cccacatctt tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttc
     2701 accacctgtt cttcgttgtc gaagggcgta acactcacta ctggaccaaa tacctctttt
     2761 tgcacgatgg catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca
     2821 ttacccttgc gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct
     2881 tctactgcct tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta
     2941 gactcgtcat ccggcgcacc agattttaac gttgccaccg cagcacccag tttttccacc
     3001 agcgtatcgt aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtcca
     3061 gcattgtaat agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca
     3121 aaaacaatca ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacgacgcg
     3181 gtatggctga tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact
     3241 ttgggatgac cggtcagcgg atcacccacc gttttgcctc tgccaaacag tatgttaatc
     3301 acgcctgccg ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta
     3361 atttctgatg gtttaagcac tacgcagttc cctgccgcca gcgccggagc aagtttccac
     3421 gcggccatca tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg
     3481 cgacggatca tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag
     3541 cgcgccgcac ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac
     3601 gcactatgca gcggtttgcc acaattacgg gattccagtt cggcaaaaac ctgaccattt
     3661 tcttcgataa catccgccag tttcagcagg cattccgcac gcgctttcgg tgtggtttgc
     3721 ccccattcag caaatgctgc atctgccgca cgcacagcag catcgacctg ctctgcggat
     3781 acctcggcaa tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc
     3841 ccttcgccgc taacccgttc tccgttaatc agtaacttat gttgcatagc ttattcctgt
     3901 atcagtggtt attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc
     3961 aaggttgtta ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt
     4021 cgcccaagct gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc
     4081 gtaacgatga tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc
     4141 gaactgagat tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc
     4201 attgacgcct caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac
     4261 accaccacta cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga
     4321 ttgatggttt taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg
     4381 ggcagcagca ataacagcga aatggcgttt ttgccgaaaa agtctcgtct ccacagcgcg
     4441 gcagctgcca gcgtccctaa caccagcgca attaatgtcg ccagcgccgc cactttaagt
     4501 gacagcgtca cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc
     4561 gtcaggccct gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg
     4621 attatcagga tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa
     4681 aacggtgcgc gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ccaggtacag
     4741 tgcgatgaga ataatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc
     4801 cccctgctgg gaataaacca tattgccgat aaaatatcct ggaggaccaa ccagctgcgg
     4861 gacgataaaa tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg
     4921 gattgccagc ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc
     4981 cgacgcctgc aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg gcaggat
//
LOCUS       DAJTRN010000180         5020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRN010000180 DAJTRN010000000
VERSION     DAJTRN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(684..1100)
                     /locus_tag="OO328_003875"
     CDS             complement(684..1100)
                     /locus_tag="OO328_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932250.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1127..1267
                     /locus_tag="OO328_003876"
                     /pseudo
     CDS             1127..1267
                     /locus_tag="OO328_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1267..2292
                     /locus_tag="OO328_003877"
     CDS             1267..2292
                     /locus_tag="OO328_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5932251.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2345..2434)
                     /locus_tag="OO328_003878"
     tRNA            complement(2345..2434)
                     /locus_tag="OO328_003878"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2398..2400),aa:Ser,seq:cga)
     gene            2528..3325
                     /gene="mtfA"
                     /locus_tag="OO328_003879"
     CDS             2528..3325
                     /gene="mtfA"
                     /locus_tag="OO328_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5932252.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3426..3501
                     /locus_tag="OO328_003880"
     tRNA            3426..3501
                     /locus_tag="OO328_003880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3459..3461,aa:Asn,seq:gtt)
     gene            3663..4925
                     /locus_tag="OO328_003881"
                     /pseudo
     CDS             3663..4925
                     /locus_tag="OO328_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1387 a         1183 c         1124 g         1326 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta attgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc gccctggtaa tgctgccacc agcgaaaatg
     1141 gttaatggaa aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg
     1201 ggatctattt acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc
     1261 gatctgatgg gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac
     1321 ccaaataaat atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc
     1381 atcaaggatg gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat
     1441 cgtgcaatga cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac
     1501 ctcagtccaa gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc
     1561 ggtaaggtgc cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga
     1621 ggggaacaga gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt
     1681 tgggggtatg agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag
     1741 gccaaaaacc gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca
     1801 cctcttcctg tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat
     1861 gtgttatcgc tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa
     1921 acaggaaaaa aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca
     1981 aaacagttac caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac
     2041 aaaggcttta acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt
     2101 ttaaccgact tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc
     2161 agtcgggata agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac
     2221 aggaaggtta aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa
     2281 tattccaagt aattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga
     2341 attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg
     2401 agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca tgatgccagg
     2461 aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag
     2521 caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc
     2581 ctggcaggaa gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa
     2641 attggtcgct cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt
     2701 tgagctgaat tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga
     2761 gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt
     2821 ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc
     2881 aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt
     2941 tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa
     3001 cggcgatcgc gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca
     3061 cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc
     3121 ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga
     3181 atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt
     3241 ctgtcaattt tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc
     3301 gttttcagcg acgaatgttc attaattaac aactttgaag attaattaac caattgaaat
     3361 ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc
     3421 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     3481 cgagtccagt cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga
     3541 tgttgattca acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc
     3601 atcgggaaaa attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc
     3661 atatatccct taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag
     3721 tctccgattc tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc
     3781 tcaaataccg tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct
     3841 ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca
     3901 acccggtaca gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa
     3961 acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc
     4021 tgcttgccag cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac
     4081 ttaaaccccg tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg
     4141 ttgcgtcccg cacacggcag cacctgagta acataatgcg ccatgcggtc catcaggagt
     4201 taatcgatac gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc
     4261 actatcctgc cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc
     4321 atcagggccg tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct
     4381 ccagtgaact gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga
     4441 tacccgcgac gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc
     4501 gaatgccgca tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg
     4561 atatcaccgg taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt
     4621 gtgaaaacac ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct
     4681 gcggtcacgg cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa
     4741 aggacgcagt agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata
     4801 ttcataaggc agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc
     4861 tggaagcatg ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc
     4921 catagtatac taacgaatcc cacactctgg caaattacca ccacctctac aggaggtggt
     4981 tctatataga cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DAJTRN010000181         4998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRN010000181 DAJTRN010000000
VERSION     DAJTRN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..359)
                     /gene="nudG"
                     /locus_tag="OO328_003882"
     CDS             complement(<1..359)
                     /gene="nudG"
                     /locus_tag="OO328_003882"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5932253.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIP"
     gene            446..1066
                     /locus_tag="OO328_003883"
     CDS             446..1066
                     /locus_tag="OO328_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5932254.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1075..2382)
                     /gene="ynjE"
                     /locus_tag="OO328_003884"
                     /pseudo
     CDS             complement(1075..2382)
                     /gene="ynjE"
                     /locus_tag="OO328_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2449..3102)
                     /locus_tag="OO328_003885"
     CDS             complement(2449..3102)
                     /locus_tag="OO328_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5932255.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3102..4573)
                     /locus_tag="OO328_003886"
                     /pseudo
     CDS             complement(3102..4573)
                     /locus_tag="OO328_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(4546..>4998)
                     /locus_tag="OO328_003887"
     CDS             complement(4546..>4998)
                     /locus_tag="OO328_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5932256.1"
                     /translation="WREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKIASGDLP
                     ASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVVWGDPSV
                     LDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
BASE COUNT         1151 a         1378 c         1323 g         1146 t
ORIGIN      
        1 ggaatgtcag caggggccag cggatattgc agcgcctctt caggtgagca ccagaccagc
       61 gcctgatgtt catgtgcctg taacgtcccg tggaagtcgg gtacgtgcca ggcatgaaga
      121 tggataatcc ggcccgaaac ttctcgctga tggctggcaa catactcacc cacagttgct
      181 tcgatgtcca attcttcgtt taactcacgc accagcgcct gctgctggct ttcatccagc
      241 tcgactttac ctccggcaaa ctcccataat cccgcctgat cgctctgggc ggggcgttgc
      301 gcgagtaaaa ttttgccatc acgttcaatg atggcggcaa caacttcaat cattttcata
      361 aattacacac gggcagataa gcgaaaaatg ccataatatc cgcactgttg ctcaatcaga
      421 aaggctttat atttaaggag tgcctgtgct agaccgccat cttcatcccc ggattaaacc
      481 gttgttgcat cagtgcgtgc gggttcttga taaaccgggc attacgccag atggtttaac
      541 attagtggga tttgccatcg gcgtgctggc tctgccgttt ctggcgctgg gctggtatct
      601 ggcggcgcta gtcgtcattt tgttgaacag gctgcttgat ggtctggacg gtgcgctggc
      661 gcggcgcaga gggcttaccg acgcgggcgg ctttcttgat atttctctcg attttctctt
      721 ttacgcgctg gtgccgtttg gctttattct tgctgcaccg gagcaaaatg cgctggcagg
      781 cggctggttg ttgtttgcgt ttatcggcac gggcagcagt tttctcgcct ttgctgcact
      841 ggcagcaaaa catcagattg ataaccccgg ttatgcacat aagtcgtttt actatctggg
      901 gggattaacc gaaggcacag aaacgatctt actgtttgtg ctgggatgtt tattccctgc
      961 atggtttgcg tggtttgcgt ggatcttcgg tgcgttgtgc tggatgacga cctttacgcg
     1021 ggtgtggagt gggtatctga cgctgaagtc gctccagcgt cagtaattgc aatgttattt
     1081 gctactgccc gggccgcgtt caccggttgc aaccggattt tttggatcgc tgctccattc
     1141 gtaccagccg ccgtcataca cggaaacatt cttccaaccc atggcgcgtg cgtacataaa
     1201 ggtttcggac gcgcgccagc cggtgccgca gtagaatgaa acttgctgat ctggtttgat
     1261 attccatgct ttccacatag cggtaatatc atcggcgcta cgcatggtgc catccgggtt
     1321 atagaaatct tccatatgcg tcgagtcgct accagcgtgt ccccaacgtg ctccggctat
     1381 ttcacctttt ggtttaatat agctgtaacc gctggtcgta ccgataaatt ctggccacga
     1441 acgaatgctc accagcgatg catcctggcg atgcagcagt ccacgggcct gttccatatc
     1501 gagcatcaac tgcggttgtg ccgggatctt cacgccgaaa tccggttccg ctttcacttt
     1561 cggtggcgtt cctcgctcaa caggcagtcc cgcgtcggac caggtttgcc agccgccatc
     1621 cagcagacgc acatctttca cgcctgcgta gagcataatt tgcgccacac gcgctccagc
     1681 gtatacgtca cgcccgtaca gaatgaccgt ggtgtcatgg cgaatgccgt gttttgccag
     1741 catcgctttt agttgttcat cagaaacttt gttccacagc ggttcgcttt ccacttcgtt
     1801 ggtatcgatg tagtcagcgc caggaatatg gctgataagg taaagcttag gagcgcccta
     1861 ggccgcttca atgactttcc agtcaccgac aggtttcgcc gtaacctctt taccttgttg
     1921 caggtcatgc agccattgcg gataaaccag ctgctcaaag tgcggcagtt tttgtagacg
     1981 ggaaggttcg cttagcgcgt cactcagaat ggagatatgc gtaaaacctg ctttttgcag
     2041 tcgcgttttg acggcgtcaa catctttgcc attaccgtac agcgccaccg gggcatcggc
     2101 tttcaggtta tgttgcttga tccacgcgtt gagctgttcg gtgctcattt tgtccagcca
     2161 gctagcagag aggtttaagg cggcaggttc atgaccagaa gggccattta aggtttgtgg
     2221 ccagccgtta taaaacgcgc tggggcgagt atctatcgct ttgccatttt gttgctgaag
     2281 ttggtcaagt gtaagaggct tcgccagttc agcggcccac gaagaagcgc aagccagccc
     2341 taaagccatt gccagcgcgg tcatttgaga aacacgtttc atcgaaaatc ccgacattgt
     2401 agttttgagt gcgccattct gaactgacgc attgagaaaa actttgcgtt atcgcagttt
     2461 gttgtaattt tctgaccact gcgccatatc cagaacagaa ctatcagcag gaacatcctg
     2521 gagatcgtgc gttacctgaa cgacggggat cgccagggcg cgaacttcgc tgaacaccca
     2581 ctggcgaaaa ttatcgcgca gagccacatc aagacggctg aatggctcat ccaggagtaa
     2641 cgcttttggc tgggcgagaa gggcgcgtag tagagcaacg cgcgctcgct gaccgccaga
     2701 caaagtggca ggatcctgat ggaaagctcc gtcaaggcct gaacgctcaa gtgcatcatt
     2761 cacggcatta cgtcgggcat tccccttaag tgtcgccggt agcgccagca gtaaattttg
     2821 cccgacgctg aactggtcaa ataacagtgc atcctgaaaa agaatgccaa tctgacgctg
     2881 tgcggtgggt agcatgtcaa tccgttgctc attgagccat agctcacctg tacaagaaaa
     2941 ctgtccggcc agtgcaccaa tcatccatga aaacagagtg gattttccac agccagacgg
     3001 ccccattaac gtgacgatgt cacctttatc aaccgtaaag ttaacgtttg tcagcaagcg
     3061 gctttctggt aaacgtagcg aaacattttt cacgcagagc attagcggat tccttgtctg
     3121 acataaccta cccattttgc gactaacgcg gtcagggcaa aaataataag cggtaacaac
     3181 agttgccata acgcctgggc ggcgagaata ccgttgctgc cgccgctgct taatgccacc
     3241 gcctcagtgg tgagcgtcgg aaaacgcccc gcgcccagcc acagcgtcgg catatactgc
     3301 gcaatactga ctgaaaatcc caccgcgaag gcaatcagca cagggcgcaa catgagtggg
     3361 catttcacgt aaaagaagat tttggcccgc gaccagccca gtgtttgcgc aatcaaaatt
     3421 aaccgtgaat caatgcgctg ccaggcgggt tgcaggataa acagcatcca cggcattacc
     3481 cacagcagat gcccccagac caccgccgtc cagcttccat ccaggttcag ccatagcgcc
     3541 agcgtgtact ggcccgccac cagtggcaga gcgggcaata gaatgggcaa ccatagccac
     3601 aactggcgac gctgtgcccc ccattccagc cacagcagta acaggagcaa agcgatgaat
     3661 gttgctacca gccccattgt cagactgttg atgagtgctt cactattgat cgtcgactga
     3721 tccgcgagga tcgccagcag aaccacacac agcacaccgg ttaagggtaa aaaactcgcc
     3781 agcgtagtgc ctggtaataa aggtgtggca ggcttgcgaa tgccatctac gcggggaata
     3841 gtgcgccgcc agctgcgcca aaacgtaggc ggcgagtaac agcatcagca gcagactggc
     3901 gagtgcgcct ttcgtttgtt gatccgcgtc gccctgagtt aaccactgcc agctaattac
     3961 tgccagcgtc ggtggattac ctggcccaag aataatcgcc acatccacga ccgaaagcga
     4021 ccaggcaaca atcgccagca tcgccattgc cagcggctgt agcccagcga atccagcaca
     4081 atgacctgct gcaacagccg tttttcgctc aatactgcca ctaagatcca cagcagaaat
     4141 gcgctttctt tcactgcaag ggtgaggccc agaccgatcc aaatcggtcc attggcggag
     4201 tgaaatacgg gaaatagtca tatagcagcc ctccgtcagc aaagagcagc agggcgctgg
     4261 tggcaaaagc cacatgggga atggcgagca gccacggcag acgggcgcac atacgctgcc
     4321 atttcggccc aggccatagc gccacaatca ccagcagggc aatcaacaat gccccgaccg
     4381 ccgcgatggt tgtcgacacc agcgttgcca gtaatgcctg cggtaactgt ggatcggcaa
     4441 ataacgcctg ccagcgtgtc aacgacaacg caggcgatat cagcgtgaga gctgccggga
     4501 tcagcggtgc ataaatcacc gccaccatcg cccacagcaa aaagattaat gcgtaccgta
     4561 acggcgtaac cattcttgtt ccagtgcatt cacccatcct gcgtgcggtt cagccagtac
     4621 cggcggcaga tcctgcggca ttcttgattg caatatttcg cgctgcccgt caggcagttt
     4681 ttgcggatcg agaacagaag gatcgcccca gacaacggga tctgctttac gcagttgcgc
     4741 atcgggtgag agcaggaaat tggcaactac cttcgccgca gcactggcat tcgcgttggc
     4801 tggaatggtg acgaaatgca cgttgccaat catcccctcg cgaaagccaa aactgtaact
     4861 gcttgccggc aaatcgccgc tggcgatttt ttgctgcgca tgtgcggggt taaaggtcag
     4921 cgacaggcgc aatgtgccgg ctttcagcag agcatccatc cgcgcgggcg aaggcgggaa
     4981 atcctttcct tcgcgcca
//
LOCUS       DAJTRN010000182         4979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRN010000182 DAJTRN010000000
VERSION     DAJTRN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            218..402
                     /locus_tag="OO328_003888"
                     /pseudo
     CDS             218..402
                     /locus_tag="OO328_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            545..766
                     /locus_tag="OO328_003889"
                     /pseudo
     CDS             545..766
                     /locus_tag="OO328_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(787..849)
                     /locus_tag="OO328_003890"
                     /pseudo
     CDS             complement(787..849)
                     /locus_tag="OO328_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1299..2711
                     /gene="ospC1"
                     /locus_tag="OO328_003891"
     CDS             1299..2711
                     /gene="ospC1"
                     /locus_tag="OO328_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5932257.1"
                     /translation="MNISETLNSANTLCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2815..2964)
                     /locus_tag="OO328_003892"
                     /pseudo
     CDS             complement(2815..2964)
                     /locus_tag="OO328_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3040..4737
                     /gene="ospD3"
                     /locus_tag="OO328_003893"
     CDS             3040..4737
                     /gene="ospD3"
                     /locus_tag="OO328_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5932258.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4854..4973
                     /locus_tag="OO328_003894"
                     /pseudo
     CDS             4854..4973
                     /locus_tag="OO328_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1708 a          909 c          941 g         1421 t
ORIGIN      
        1 caaagcgcag aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga
       61 catagcgggg aagtttgctt ctttgagagg cgggtttacg tttacgggat ttagctgaac
      121 gggccatata accacctgaa agacaatgac attgcctgtt tttataacgg taattgcaga
      181 ccatgacaaa ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat
      241 atgccggcag cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt
      301 tatgggggcg gtgaagaatt atctaacctt gtcttgttac atccaaattg ccatcgacag
      361 ttacacagtt gtgaagccgg ctctctcaaa aagggcttat aaaggcttga gcggtgtacc
      421 gggaaaccgg tatgcaccgt tcttagggga gggcagtcca gcaatgaact gtccttaccc
      481 gacaaacatc caaaacgaac ggaacgtgct ggaatccgca tataacccgt tgtaaagtga
      541 ggaaatggac acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca
      601 acaggacact atccaccaga tggctgaata caaccgcctg ctctcacagc ggatggcggc
      661 ttatgcttcc gaaatcaacc gactgaaggc gctggttgcg aaactgcagc gtatgcagtt
      721 cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg cagattccat tttcacgggc
      781 gatctacgcc acacaggctc tgggttgcag tgactgctcc attaaggcga ttttgaactc
      841 ataagggaag taacaaaaaa actgcaaaag tacaataatt atatatggcc aaactaacct
      901 ctattaaaaa acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat
      961 ttcattgcag aatacacatt ccactaactg gaagcactcc ataagaggaa tcagagaaag
     1021 tatcgttttt ttatagtaaa attcattgct gttcaataaa attgatatag ttattaaatt
     1081 atgaaaataa tttcacaaaa aagattaact tgagttctta agcatcggat tttagatatt
     1141 ttgaaatatc tggcatcaca taacatcaaa agtatataat actcttatct aattacatgc
     1201 agtatattgt atatatactc aaaaattgta attcataccc tgctatatct tgtagggaaa
     1261 gatattaatt aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga
     1321 actcagcaaa taccctatgc aatatagatt ctatggataa cagattacat acattgtttc
     1381 caaaagtgac atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa
     1441 aagatagtgc aaatattatt aaaagtttct ttaggaaaac tatagcagca cagagttata
     1501 gtagaatgtt ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat
     1561 cagacgctaa agcctcattt aaggctattg agcaccttga cagattatcg aagcattata
     1621 tatctgaaat aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc
     1681 taattattaa ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa
     1741 ttttaaacat taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca
     1801 catacgctga tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa
     1861 tctctaacca tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg
     1921 caaatgcgta tatcattgat catgactctc catatggata tatgacatta accgatcact
     1981 ttgataatgc tattccacct gttttttacc atgagcacca atcattttta gataaatttt
     2041 cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac
     2101 caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta
     2161 gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg
     2221 atctggatag aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa
     2281 gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag
     2341 catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc
     2401 tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg
     2461 agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac
     2521 ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg
     2581 atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa
     2641 aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata
     2701 aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg
     2761 atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt
     2821 cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag
     2881 cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg
     2941 acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa
     3001 gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc
     3061 ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca
     3121 atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta
     3181 ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta
     3241 gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt
     3301 gcttttccgg atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat
     3361 gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt
     3421 ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat
     3481 cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt
     3541 cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac
     3601 ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt
     3661 tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg
     3721 tcctccataa tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg
     3781 cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt
     3841 tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg
     3901 cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa
     3961 acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga
     4021 cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac
     4081 ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg
     4141 accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa
     4201 aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc
     4261 aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt
     4321 ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat
     4381 gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt
     4441 ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg
     4501 aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa
     4561 ggcccaaatg gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata
     4621 aaaacttata tggaaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac
     4681 ttggacaaaa aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata
     4741 ttatctcgtg gtgatacact gaatttgagg taaccggctc atttaaaccg tctggtctgt
     4801 tacctccggt tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat
     4861 accggacctg gatcactgaa gctttacgcc ttcatttcga agaacattta ccacgggttg
     4921 tggccggacg tcgcctgggc gtaccaaaat caactgtctg tggtatgttc gtgtgaacc
//
LOCUS       DAJTRN010000183         4819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRN010000183 DAJTRN010000000
VERSION     DAJTRN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1101)
                     /locus_tag="OO328_003895"
     CDS             complement(<1..1101)
                     /locus_tag="OO328_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5932259.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO328_003896"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO328_003896"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5932260.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO328_003897"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO328_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5932261.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO328_003898"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO328_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5932262.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO328_003899"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO328_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5932263.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO328_003900"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO328_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5932264.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="OO328_003901"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="OO328_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5932265.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..>4819)
                     /locus_tag="OO328_003902"
     CDS             complement(4502..>4819)
                     /locus_tag="OO328_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5932266.1"
                     /translation="ESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFND
                     PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDA
                     LSD"
BASE COUNT         1196 a         1215 c         1144 g         1264 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattc
//
LOCUS       DAJTRN010000184         4615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRN010000184 DAJTRN010000000
VERSION     DAJTRN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            191..1171
                     /gene="rluD"
                     /locus_tag="OO328_003903"
     CDS             191..1171
                     /gene="rluD"
                     /locus_tag="OO328_003903"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5932267.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1168..1899
                     /gene="pgeF"
                     /locus_tag="OO328_003904"
     CDS             1168..1899
                     /gene="pgeF"
                     /locus_tag="OO328_003904"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5932268.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2029..4602
                     /gene="clpB"
                     /locus_tag="OO328_003905"
     CDS             2029..4602
                     /gene="clpB"
                     /locus_tag="OO328_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5932269.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1156 a         1107 c         1271 g         1081 t
ORIGIN      
        1 cccgccaaaa acaggcttag tgtggcggct gccaccagat atttcatgcg cgtcatgacg
       61 ttttgacttt cctcaaaatg taatacggga gattctctgt tcctgcaccc ggttaagacc
      121 agctacaata gcacactata ttaaacggca aagccgtaaa accccaacga taaacgaaga
      181 agcagtatat atggcacaac gagtacagct cactgcaacg gtgtccgaaa accaactcgg
      241 tcaacgctta gatcaggctt tggccgaaat gttcccggat tattcacgtt cgcgaataaa
      301 agaatggatc ctcgaccagc gcgtgctggt aaacggcaaa gtttgtgata agccgaaaga
      361 aaaagtattg ggtggcgagc aggttgccat caacgctgag attgaagaag aagcgcgttt
      421 tgaaccgcag gatatccctc tggatatcgt ctatgaagat gaagatatca tcgtcattaa
      481 taaaccgcgc gacctagtgg tacatcctgg cgcgggtaac ccggatggca cggtactgaa
      541 tgcgttgctt cattactatc cgcccattgc cgatgtacca cgtgcgggca tcgtccatcg
      601 tctggataaa gacaccactg gcctgatggt tgtggcaaaa accgttccgg ctcagacgcg
      661 tttagtcgaa tctttgcaac ggcgtgaaat tactcgtgag tatgaagcgg tggcgattgg
      721 tcatatgacc gctggcggta cagtagatga acccatcagc cgccacccga ccaaacgtac
      781 tcacatggcg gtgcatccga tgggcaaacc ggcggtgact cactatcgca tcatggaaca
      841 cttccgtgtg cacacgcgtc tgcggttgcg tctggaaact ggacgtacgc accagatccg
      901 cgtgcatatg gcccatatca ctcatccgct ggtgggcgat ccggtttatg gtggccgtcc
      961 gcgtccgcca aaaggtgctt cggaagcatt tatctccacg ctgcgtaagt ttgaccgcca
     1021 ggcgcttcat gcaaccatgc tgcgtcttta tcacccgatc tccggcatcg aaatggaatg
     1081 gcatgcgcct attccacaag atatggtgga gctgattgag gtgatgcgcg ccgatttcga
     1141 agagcataag gatgaagtgg actggttatg agtaagctga ttgtcccgca gtggccgctg
     1201 ccaaaaggtg ttgcggcctg tagctccact cgtatcggcg gcgtgagctt gcccccgtat
     1261 gactcactca acctcggtgc ccattgtggc gataacccgg atcacgttga ggagaatcgc
     1321 aagcgacttt ttgctgcggg caatttgcct tctaaaccgg tctggcttga gcaggtacac
     1381 ggcaaagatg tgcttaagct tactggcgaa ccttatgcct caaaacgagc agatgcctct
     1441 tatagcaata cgcctggcac ggtttgcgca gtgatgactg ccgactgcct ccctgtgctg
     1501 ttttgcaatc gagcgggaac ggaagtcgcc gccgttcatg ctggctggcg tggactgtgc
     1561 gcaggcgtgc tggaagagac ggtttcctgt tttgctgata aacctgaaaa cattctcgcc
     1621 tggttagggc cggcaattgg tccacgcgcg ttcgaagtgg gggcggaggt tcgcgaggcg
     1681 tttatggcag tagacgctaa agcaagtgca gctttcattc agcatggtga taagtatctg
     1741 gcggatattt atcagcttgc ccggcagcgt ctggcgaacg tgggtgttga gcaaattttc
     1801 ggcggcgacc gttgtacata tacggaaaat gagactttct tctcttatcg tcgcgacaag
     1861 accaccggtc gtatggcaag tttcatttgg ctgatataac ctaaagaatc aagacgatcc
     1921 ggtacgcgtg attttctttt cacattaatc tggtcaataa ccttgaataa ttgagggatg
     1981 acctcattta atctccagta gcaactttga tccgttatgg gaggagttat gcgtctggat
     2041 cgtcttacta ataaattcca gcttgctctt gccgatgccc aatcacttgc actcgggcac
     2101 gacaaccaat tcatcgaacc acttcattta atgagcgccc tgctgaatca ggaagggggt
     2161 tcggttagtc ctttattaac atccgctggc ataaatgctg gccagttgcg cacagatatc
     2221 aatcaggcat taaatcgttt accgcaggtt gaaggtactg gtggtgatgt ccagccatca
     2281 caggatctgg tgcgcgttct taatctttgc gacaagctgg cgcaaaaacg tggtgataac
     2341 tttatctcgt cagaactgtt cgttctggcg gcacttgagt ctcgcggcac gctggccgac
     2401 atcctgaaag caacaggggc gaccaccgcc aacattactc aagcgattga acaaatgcgt
     2461 ggaggtgaaa gcgtgaacga tcaaggtgct gaagaccaac gtcaggcttt gaaaaaatat
     2521 accatcgacc ttaccgaacg agccgaacag ggcaaactcg atccggtgat tggtcgtgat
     2581 gaagaaattc gccgtaccat tcaggtgctg caacgtcgta ctaaaaataa cccggtactg
     2641 attggtgaac ccggcgtcgg taaaactgcc atcgttgaag gtctggcgca gcgtattatc
     2701 aacggcgaag tgccggaagg attgaaaggc cgccgcgtac tggcgctgga tatgggcgcg
     2761 ctggtggctg gggcgaaata tcgcggtgag tttgaagaac gtttaaaagg cgtgcttaac
     2821 gatcttgcca aacaggaagg caacgtcatc ctgtttatcg acgaattaca taccatggtc
     2881 ggcgcgggta aagccgatgg cgcaatggac gccggaaaca tgctgaaacc ggcgctggcg
     2941 cgtggtgaat tgcactgcgt aggtgccacg acgcttgacg aatatcgcca gtacatcgaa
     3001 aaagatgctg cgctggaacg tcgtttccag aaagtgtttg ttgccgagcc ttctgttgaa
     3061 gataccattg cgattctgcg tggcctgaaa gaacgttacg aattgcacca ccatgtgcaa
     3121 attactgacc cggcaattgt tgcagcagcg acgttgtctc atcgctacat tgctgaccgt
     3181 cagctgccgg ataaagccat cgacctgatc gatgaagcag catccagcat tcgtatgcag
     3241 attgactcaa aaccagaaga actcgaccga ctcgatcgtc gtatcatcca gctcaaactg
     3301 gaacaacagg cgttaatgaa agagtctgat gaagccagta aaaaacgcct ggatatgctc
     3361 aacgaagaac tgagcgacaa agaacgtcag tactccgagt tagaagaaga gtggaaagca
     3421 gagaaggcat cgctttctgg tacgcagacc attaaagcgg aactggaaca ggcgaaaatc
     3481 gctattgaac aggctcgccg tgtgggggac ctggcgcgga tgtctgaact gcaatacggc
     3541 aaaatcccgg aactggaaaa gcaactggaa gccgcaacgc agctcgaagg caaaactatg
     3601 cgtctgttgc gtaataaagt gaccgatgcc gaaattgctg aagtgctggc gcgttggacg
     3661 gggattccgg tttctcgcat gatggaaagc gagcgcgaaa aactgctgcg tatggagcaa
     3721 gaactgcacc atcgcgtcat tggtcagaac gaagcggttg atgcggtatc taacgctatt
     3781 cgccgtagcc gtgcggggtt ggcggatcca aatcgcccga ttggttcatt cctgttcctc
     3841 ggcccaacgg gtgtggggaa aactgagctt tgtaaggcgc tggcgaactt tatgtttgat
     3901 agcgacgagg cgatggtccg tatcgatatg tccgaattta tggagaaaca ctcggtgtct
     3961 cgtctggttg gtgcgcctcc gggatatgtc ggttatgaag aaggtggcta cctgaccgaa
     4021 gctgtgcgtc gtcgtccgta ttccgtcatc ctgctggatg aagtggaaaa agcgcatccg
     4081 gatgtcttca atattctgtt gcaggtactg gatgatgggc gtctgactga cgggcagggg
     4141 agaacggtcg acttccgtaa tacggttgtc attatgacct ctaacctcgg ttccgatctg
     4201 attcaggaac gcttcggtga actggattat gcgcacatga aagagctggt gctcggtgtg
     4261 gttagccata acttccgtcc ggaattcatt aaccgtattg atgaagtggt ggtcttccat
     4321 ccgctgggtg aacagcacat tgcctcgatt gcgcagattc agttgaaacg tctgtacaaa
     4381 cgtctggaag aacgtggtta tgaaatccac atttctgacg aggcgctgaa actgctgagc
     4441 gagaacggtt acgatccagt ctatggtgca cgtcctttga aacgtgctat ccagcagcag
     4501 atcgaaaacc cgctggcaca gcaaatactg tctggtgaat tggttccggg taaagtgatt
     4561 cgcctggaag ttaatgaaga ccggattgtc gctgtccagt aatgataaaa cgagc
//
LOCUS       DAJTRN010000185         4613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRN010000185 DAJTRN010000000
VERSION     DAJTRN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(96..560)
                     /locus_tag="OO328_003906"
     CDS             complement(96..560)
                     /locus_tag="OO328_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5932270.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(557..1432)
                     /gene="nanK"
                     /locus_tag="OO328_003907"
     CDS             complement(557..1432)
                     /gene="nanK"
                     /locus_tag="OO328_003907"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5932271.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1429..2118)
                     /locus_tag="OO328_003908"
     CDS             complement(1429..2118)
                     /locus_tag="OO328_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5932272.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2166..3656)
                     /gene="nanT"
                     /locus_tag="OO328_003909"
     CDS             complement(2166..3656)
                     /gene="nanT"
                     /locus_tag="OO328_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5932273.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3766..>4613)
                     /gene="nanA"
                     /locus_tag="OO328_003910"
     CDS             complement(3766..>4613)
                     /gene="nanA"
                     /locus_tag="OO328_003910"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5932274.1"
                     /translation="DQQQALDKASLRRLVQFNIQQGIDGLYVGGSTGEAFVQSLSERE
                     QVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDAVSAVTPFYYPFSFEEH
                     CDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPGVGALKQTSGDLYQMEQ
                     IRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQGIVKALKEGDIQTAQK
                     LQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGPVDEKYLPELKALAQQL
                     MQERG"
BASE COUNT         1105 a         1336 c         1187 g          985 t
ORIGIN      
        1 aagaatacaa cgtcaggtaa tggcacagag cggtccgtca gttcaggttt ggctctgtgc
       61 caaagatggg tcaggtcaca cctgatctaa taaatttaag ccattaaatc agccttaacc
      121 ttcaccacca cctttttaat ctcacccggc tcgccaacaa cgcatcccgg cttatgcggc
      181 tctcctggca taaacacggc gaacattccc ggtttcagaa tgatggtttg ctcgttttca
      241 atggcgctac aaagctggta atcatcttca tggtggaact cttcacattg gcgcgcagtg
      301 cctgccatgc caaacagaat ccgttcctca ccgtttaata acagctggat atcaatgtat
      361 tgctcgtgca attccgcttt tttctcgacg ggcgattgag tgttaaacgt catgacattc
      421 ataaaaatat tgtcgccctg taattcgtaa cgacccggcg ctttttcttg cggtctggca
      481 gctaaggcca gcgttaacgc gtcctgtaac gcaggatgta acccagcaga cggtaatgac
      541 tgtacttcac ccatcatcat aatttttctc cctgggccaa cagcgcagcc ccaagtaaac
      601 ctgcatcatg gcggtaatgc gccgccagta aatcaacatg aaatgccgct ggctcctgcg
      661 ccagatacat ttccaccaac gccagatacc cttctgccag accaacgctg ccaccaacca
      721 ccacgcactg gcaatcagtt gtggctttaa tatcagcgat cagccttgca agcgtacgtg
      781 cggagcgatg aatcagttgc tgcgcctgct catcgccctg cccggcgcgt gtgaaaatag
      841 ttctcgcatc cgcgccagcc agctcgccct gcgctgccgc cgcaatgccg cgaccagaag
      901 caattgcttc cacgcaacct gtgcgtccac agccgcagac tgggccgtgt ggatcggcaa
      961 gcgtatgccc gatgtgcccc gccagaccgc cagggccggt aagcagtttg ccgccgctca
     1021 ctacaccgcc gccgacgcca gtggaaacgg tgataaagac catatcggtt atatcgcctt
     1081 ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc gttaatggca atggtcggca
     1141 aattggtaag ttgttccagc gttttgacta acggaaagtg tagcaatcca ccgagattat
     1201 gcggattcag cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg atggcaaccc
     1261 gctgcgcatg agcttgcaac ggagagacta atgcggataa ggcatcacgc aaggcttctg
     1321 gtgtctggct ggctggcgta ggaagttcac gacgatcgcg gatctgcccg tcagcgccaa
     1381 tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc cagtatggtc atagcaccgc
     1441 ctttttcatc gctgtgttgt accactgaca aatgtgttca agacgcgtaa tggcagaacc
     1501 gaccgtcacc gcccacgcgc cgtggcgcat cgcatctgcc gcctgagcag gcgtgttgta
     1561 acgcccttcg gcaatcaccc gacatccggc ttcgctcaac gttttcacca gcgccagatc
     1621 cggctcttct ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa taatttcggc
     1681 tcccagcttt tggcatgcca ggccgtcttc cggcgttgag cagtcggtca tcgccagtaa
     1741 accgtgatga tggatacgtg ccagcagcgt ttcaacaggc accggacgcg ggcggtcggt
     1801 gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc gcatcgacat cttcaatata
     1861 ggccgtgatg cgtaccggag aatcctccag atcgcgtttc acaattccaa taatcggcac
     1921 gctcaccacc gcacgcgtgg cttgcagatt tgccacacct tcgatgcgaa tggcaaccgc
     1981 gcccgcctgc tctgccgcta atgccatggc tgcgacgatt tcgggtttat cgagcgggct
     2041 gtcaggaacc ggctggcagg agacaatcag gccaccgtta gcagcgattt tttgatccag
     2101 ttgtgcaagt aacgacatac atcttccctt agcgaaaggc ccggtacata gaccgggcaa
     2161 caggattaac ttttggtttt gactaaatcg tttttggcgc tgccaaatgg cacggcaccg
     2221 ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg cttccgggcg cagccaacgc
     2281 tgaacgcggg aaggcatatc cagaccaatc agcaggatca ccacaaatgt caggctgaac
     2341 gagagcgatg ccagcgcagt acccagatcc agacgttgag cgatcaacgc gccgatgatt
     2401 ggggccagtg cgccacccaa tgcgccaacg ttgtaggtaa agcccaggcc cgctgcacgc
     2461 tggtcggtat cgaaataacc gccaatcagt ttcggtaaga tcccggcgat cccttgtcca
     2521 agcatttgct ggaagaacag taacagaccg agcacccaga cgtttgcgcc gccaatcgca
     2581 aataccggaa taatcagcag ctgcgaggcc agcaggctac aaacgtacgc tttgcgggtt
     2641 cccagccagt caccgaggaa gccacctacg cagcatccca cggccgcgcc aaagccacta
     2701 aagaacagca cattggcgac agtatgcggg ttataagcca gatcggtttt cagatacgtt
     2761 ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa acaacacgac caccatcagc
     2821 attacgcccg ttggccagcg tttacccgta ctttgcacca taaagctgat aaagattgcg
     2881 gcgcataaca gcccgagaac agcgacgatt gcagcatttt gcaggttacc ggcgaagcag
     2941 aaccacagcg cagtagccgc cgccagtgtc attacgatat tggcaatgcg atgttcacca
     3001 cggtagagaa tatccaccat tgtgcgtact ggtgctttac ctgcgtgttt ctctttccag
     3061 tcttccgctt ccgggatgtt tttacgcagc cagagagcaa agatgattgg caaaatgccg
     3121 ataaagaaca gcgcacgcca accccagacc ggaaccacca ggctatagac ctgagcggca
     3181 acgacggccc ccacagagaa gcctgaaatc aaaaaaccac tggctttgtt acgcagatgt
     3241 tttggccagc tttcaatgac ataggtggcg ctggaaccgt attcacccgc catccccatg
     3301 ccgatgacca gacgagcgat aaacatggtg atgtagcctg gcgcaaagcc gcaggccagc
     3361 gtcccggccg agaagagaac gatgctggtg accattgcca gacgacgccc gtagcggtca
     3421 cccatagcgc cgagcatcag gccgccgaac cagcgagaga taaaggctgc ggagatcaga
     3481 cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta cttcggtgag taccagggcg
     3541 attaaaacga aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga aaatgcgcgc
     3601 cattgtgcac ggttgagatg gcgataccac gggatattct gggttgtagt actcattgtg
     3661 agtctcccgc ggtgggcgat gcccacacgc tttggtatga aaattgtagg gtacagatgc
     3721 gtttatttcc cctcacccgg taggggcgag cgaggggaaa acaactcacc cgcgctcttg
     3781 catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta ccggtccaaa
     3841 cggtttgcgg cacagcggca cagaaacgac atccatataa tgcaggacag ttttcaggcc
     3901 gcggaatacg cccgttttga tcagtaaatc aatgactttg ttgcactcag tttgcagttt
     3961 ctgcgcggtc tggatatcgc cttctttcag cgccttcacg atcccctgat atcgccagcc
     4021 catgatgttg taagtactgc cgataccacc atcagcgcct gccagcaagc cggaagcgaa
     4081 gatttcgtcg taaccgttat agagcaccag atcaggatgt tcacgacgga tctgttccat
     4141 ctgatagaga tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg taaccagggt
     4201 gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt acaccaccat
     4261 cggcaaacca tccgctgaat caataattgc ccgatagtga tcgcagtgtt cttcaaagct
     4321 gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac gtttagccga
     4381 tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga tgagtttaat
     4441 cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg aaaggctttg
     4501 tacaaaggct tcgccggtcg aaccacccac gtataaaccg tcgatgccct gctgaatatt
     4561 gaactgaacc aggcggcgca gactcgcttt atccagtgct tgttgttgat caa
//
LOCUS       DAJTRN010000186         4550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRN010000186 DAJTRN010000000
VERSION     DAJTRN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1222
                     /locus_tag="OO328_003911"
     CDS             <1..1222
                     /locus_tag="OO328_003911"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5932275.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="OO328_003912"
     CDS             1222..1956
                     /locus_tag="OO328_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5932276.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2423..3184
                     /locus_tag="OO328_003913"
     CDS             2423..3184
                     /locus_tag="OO328_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5932277.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3228..4307
                     /locus_tag="OO328_003914"
     CDS             3228..4307
                     /locus_tag="OO328_003914"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5932278.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4304..>4550
                     /locus_tag="OO328_003915"
     CDS             4304..>4550
                     /locus_tag="OO328_003915"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5932279.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDWICPY"
BASE COUNT         1247 a          866 c         1121 g         1316 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt
     2221 gtaggctttt gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata
     2281 acaaaattaa gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag
     2341 taaggcgttg taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt
     2401 tgtcgaaatg gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg
     2461 cttgtcgttt ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca
     2521 aaacacttca gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc
     2581 ggatggcgca gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa
     2641 agtctttgaa gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag
     2701 gcttaatcca ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga
     2761 cagggaaaaa ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg
     2821 ggccgagaaa gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt
     2881 acatacctgc ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc
     2941 gagtgagaag ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc
     3001 gttctacatc aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta
     3061 tattgcgccg ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa
     3121 atggtctgtg attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc
     3181 ataatgtatt acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac
     3241 tgtcattaat tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat
     3301 ttgattcttc acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg
     3361 gagagcaact ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg
     3421 ttggggagtt tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt
     3481 taaccgttga tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg
     3541 acaataaaaa gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg
     3601 attttgatag ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa
     3661 gtagtttagc accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc
     3721 agacaaatat tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt
     3781 atggcaggtt cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat
     3841 ggagcaagga acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag
     3901 gcataaatgc gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt
     3961 ttgacagcat accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg
     4021 aggacagcta ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta
     4081 ttaaacaaaa tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg
     4141 ataatttacc aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg
     4201 gcgaggagca gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat
     4261 atttcaaata tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt
     4321 ctccgtgggc gaaatcacgg cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg
     4381 gattcaaaac gccactcatt ttaatgcaat atctacgggt attggcattt ctctggggct
     4441 gttagggagt ttatcgacag atattacgcg ttcgattgcg aatttatatt atgggaataa
     4501 atatcgtatt cgttatagta aatccattag tgattggatt tgcccctata
//
LOCUS       DAJTRN010000187         4449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTRN010000187 DAJTRN010000000
VERSION     DAJTRN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..528
                     /locus_tag="OO328_003916"
     CDS             <1..528
                     /locus_tag="OO328_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5932280.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            725..2164
                     /gene="aldA"
                     /locus_tag="OO328_003917"
     CDS             725..2164
                     /gene="aldA"
                     /locus_tag="OO328_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5932281.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2206..3206)
                     /gene="gap"
                     /locus_tag="OO328_003918"
                     /pseudo
     CDS             complement(2206..3206)
                     /gene="gap"
                     /locus_tag="OO328_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="OO328_003919"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="OO328_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5932282.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="OO328_003920"
     CDS             4170..4343
                     /locus_tag="OO328_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932283.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT         1140 a         1025 c         1117 g         1167 t
ORIGIN      
        1 cgcaccactg gcagagcaga agcgaccatc ctggtggata tcgctcatca gttctggcat
       61 attccgcatg aaaaaatctg gattgaagac cagtcaacaa actgcggtga aaacgcacgc
      121 tttagcatca cgctattgaa tcaggccgta gaacgagttc atacggctat cgttgtacag
      181 gaccccacca tgcagcggcg cacgatggca acgttccgcc gtatgaccgg ggacaatccc
      241 gatgcaccac gctggttaag ttatcccgga ttcgttcctc agttaggaaa taacgcagac
      301 agtgtaatct ttattaatca gttacaagga ttatggccgg ttgagcgtta tctctcacta
      361 ctcactggcg agctgccgcg tttacacgat gatagcgatg gctacggtcc ccgcgggcga
      421 gattttatcg ttcacgttga ttttccggca gaagtcatcc atgcatggca aacgctgaaa
      481 catgatgcgg tgctcatcga ggcgatggaa agtcgctcgt tacgttaaaa attgcccgtt
      541 tgtgaaccac ttgtttgcaa acgggcatga ctcctgactt ttatttctgc cttttattcc
      601 ttttacactt gtttttatga agccctccac agacttgtcc tttcccgatg ccgtctctct
      661 gatgattgat gttaattaac aatatattca ccgaaaacaa acatataaat cacaagagtc
      721 gcccatgtca gtacccgttc aacatcctat gtatatcgat ggacagtttg ttacctggcg
      781 tggagacgca tggattgatg tggtaaaccc tgctacagag gctgtcattt cccgcatacc
      841 cgatggtcag gccgaggatg cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga
      901 atgggaagcg ttgcctgcta ttgaacgcgc cagttggttg tgcaaaatct ccgccgggat
      961 ccgcgaacgc gccagtgaaa tcagtgcgct gattgttgaa gaagggggca agatccagca
     1021 gctagctgaa gtcgaagtgg cttttactgc cgactatatc gattacatgg cggagtgggc
     1081 acggcgttac gagggcgaga ttattcaaag cgatcgtccg ggagaaaata ttcttctgtt
     1141 taaacgtgcg cttggtgtga ctaccggcat tctgccgtgg aactttccgt tcttcctcat
     1201 tgcccgcaaa atggctcccg ctcttttgac cggtaatacc atcgtcatta aacccagtga
     1261 atttacgcca aacaatgcga ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg
     1321 cggcgtgttt aaccttgtac tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa
     1381 cccaaaggtc gcaatggtca gtatgacagg cagcgtctct gcaggtgaga agatcatggc
     1441 gactgcggcg aaaaacatca ccaaagtgtg tctggaattg ggtggtaaag caccggctat
     1501 cgtaatggac gatgccgatc ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat
     1561 taatagtggg caagtgtgta actgtgcaga acgtgtttat gtacagaaag gcatttatga
     1621 tcagttcgtc aatctgctgg gtgaagcgtt gcaggcggtt caatttggta accccgctga
     1681 acgcaacgac attgcgatgg ggccgttgat taacgccgcg gcgctggaaa gggttgagca
     1741 aaaagtggcg cgcgcagtag aagaaggggc gagagtggcg ttgggtggca aagcggtaga
     1801 ggggaaagga tattattatc cgccgacatt gctgctggat gttcgccagg aaatgtcgat
     1861 tatgcatgag gaaacctttg gcccggtgct gccagttgtc gcatttgaca cgctggaaga
     1921 tgctatctca atggctaatg acagtgatta cggcctgacc tcatcaatct atacccaaaa
     1981 tctgaacgtc gcgatgaaag ccattaaagg gctgaagttt ggtgaaactt acatcaaccg
     2041 tgaaaacttc gaagctatgc aaggtttcca cgccggatgg cgtaaatccg gtattggcgg
     2101 cgcagatggt aaacatggcc tgcatgaata tctgcagacc caggtggttt atttacagtc
     2161 ttaatgagtg aaagaggcgg aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa
     2221 tttttcgagg gtgcgaataa gctgcgtgac gaagccatat tcgttatcgt accaggcgac
     2281 cgttttcacc agttgtaaat cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac
     2341 tgaaccgaaa tggctgccaa tgatatcgga agagactatt tcttcatcgg tataaccaaa
     2401 tgactcgtta ttggtggttg cttgtttaag tgcgttattc acctcttcgg cagtcacttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacct
//
LOCUS       DAJTRN010000188         4370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTRN010000188 DAJTRN010000000
VERSION     DAJTRN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            38..112
                     /gene="tnaC"
                     /locus_tag="OO328_003921"
     CDS             38..112
                     /gene="tnaC"
                     /locus_tag="OO328_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5932284.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            333..1748
                     /gene="tnaA"
                     /locus_tag="OO328_003922"
     CDS             333..1748
                     /gene="tnaA"
                     /locus_tag="OO328_003922"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5932285.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1839..3086
                     /gene="tnaB"
                     /locus_tag="OO328_003923"
     CDS             1839..3086
                     /gene="tnaB"
                     /locus_tag="OO328_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5932286.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3218..>4370
                     /gene="mdtL"
                     /locus_tag="OO328_003924"
     CDS             3218..>4370
                     /gene="mdtL"
                     /locus_tag="OO328_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5932287.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAH"
BASE COUNT          987 a         1042 c         1080 g         1261 t
ORIGIN      
        1 ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac atgtatgtgt
       61 gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt aatttaccct
      121 tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta gtttgctata
      181 tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt agtaaatgat
      241 ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat tcacagggat
      301 cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat ctccctgaac
      361 cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat cgtgaagaag
      421 caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg tttatcgatt
      481 tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg atgatgcgcg
      541 gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca gtgaaaaata
      601 tctttggtta tcaatatact attccgactc accagggccg tggcgcagag caaatctata
      661 ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg
      721 cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac ggctgtaccg
      781 tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac tttaaaggca
      841 actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat aacgtgccgt
      901 atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca ctggcaaact
      961 taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg gactccgcac
     1021 gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa gactggacca
     1081 tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg tccgccaaga
     1141 aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg
     1201 tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca tatggcggct
     1261 tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg aatctcgact
     1321 ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa gagattggcg
     1381 ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa ctgctgccgc
     1441 atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg
     1501 gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa accggtaaac
     1561 aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa
     1621 cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg gcgaatatta
     1681 aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca aaactgaaag
     1741 aagtttaatt aatactacag agtggctata aggatgttag ccactctctt accctacatc
     1801 ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa
     1861 agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt ggaggtatgt
     1921 ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt atccttatca
     1981 ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat ttaaattatc
     2041 ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc tggaacatta
     2101 tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat atctctgcta
     2161 atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct aatccacgta
     2221 ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt tcgttagccg
     2281 ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg
     2341 gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc accactgcgg
     2401 gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg tcatttggtt
     2461 tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat aagttaatca
     2521 aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg ctctattgca
     2581 cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc ggcaacattg
     2641 attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag ttttgcctgc
     2701 tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg gggttgttcg
     2761 attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc aaaaccgtgc
     2821 tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc tttatttacg
     2881 ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc gcagtgcttg
     2941 caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc ggcaatctta
     3001 ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc ggcaacgtct
     3061 ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg ctggcttctt
     3121 gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct tcaccacgta
     3181 tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt tgatttgtag
     3241 ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg gtttaccgcg
     3301 catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct ccgtatatct
     3361 ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt cagggagaaa
     3421 gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct gttcactggc
     3481 tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg caggctgttg
     3541 ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc gggctaaagt
     3601 gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag tgctcggaca
     3661 tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa tgatgggcat
     3721 cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc
     3781 ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc tcagccgtgt
     3841 tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat cgccggtatt
     3901 gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg cgctgaccgc
     3961 tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt ttaagccacg
     4021 tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc ttgccgtttc
     4081 accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt tctcggtagg
     4141 ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg gcgtagccag
     4201 ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc tggccgcggt
     4261 ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta gcatagtgag
     4321 cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
//
LOCUS       DAJTRN010000189         4300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRN010000189 DAJTRN010000000
VERSION     DAJTRN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(327..572)
                     /locus_tag="OO328_003925"
     CDS             complement(327..572)
                     /locus_tag="OO328_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932288.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1349..2191)
                     /locus_tag="OO328_003926"
     CDS             complement(1349..2191)
                     /locus_tag="OO328_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5932289.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2276..2473)
                     /locus_tag="OO328_003927"
     CDS             complement(2276..2473)
                     /locus_tag="OO328_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932290.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2493..2981)
                     /locus_tag="OO328_003928"
     CDS             complement(2493..2981)
                     /locus_tag="OO328_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932291.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2978..3355)
                     /locus_tag="OO328_003929"
     CDS             complement(2978..3355)
                     /locus_tag="OO328_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5932292.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3402..3779)
                     /gene="cbtA"
                     /locus_tag="OO328_003930"
     CDS             complement(3402..3779)
                     /gene="cbtA"
                     /locus_tag="OO328_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR5932293.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3859..4080)
                     /locus_tag="OO328_003931"
     CDS             complement(3859..4080)
                     /locus_tag="OO328_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5932294.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4167..>4300)
                     /locus_tag="OO328_003932"
     CDS             complement(4167..>4300)
                     /locus_tag="OO328_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932295.1"
                     /translation="PSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         1023 a         1018 c         1112 g         1147 t
ORIGIN      
        1 ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta acaaattcaa
       61 tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta atgctctaac
      121 ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct tttaatctca
      181 ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt ttgatgttga
      241 tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac tgttgcgata
      301 taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac cagaccctga
      361 ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt gtgtctgcct
      421 ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag ctgcacgttg
      481 agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca gtgcatccgc
      541 tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt gactgtcgtg
      601 ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta aattgctttc
      661 caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata tgcaatgggc
      721 tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa cgttctatct
      781 ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc ctatggatca
      841 tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat cagagtgaaa
      901 aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg tatgcgtttt
      961 acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt agccaggtgc
     1021 tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc acagtgacca
     1081 gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc agtgacatca
     1141 gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca gcccgtaggc
     1201 tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat catattgata
     1261 ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg cagtcccttt
     1321 tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga aagtacctgg
     1381 ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg tgtgcagatc
     1441 ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta ccctgaacgg
     1501 actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg atcggtttgc
     1561 cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt tgcccggtga
     1621 taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt ttactgccaa
     1681 atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg ttgatcactc
     1741 cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc agaatgttcg
     1801 cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg tcccgggtct
     1861 gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag agttcgcgat
     1921 caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc cagcaaccgc
     1981 agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata ctttctgtca
     2041 ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat gtactctttc
     2101 gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc ccgttgacca
     2161 cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc agataaacga
     2221 aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt aagactcacc
     2281 ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg gcctgttcaa
     2341 tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag ataatccctg
     2401 attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc gtttctgtgg
     2461 ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg gaaccccggc
     2521 agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg ggtgagagtg
     2581 acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag aaccgggtac
     2641 gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca ttctgtttcg
     2701 ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt ttccgtgata
     2761 tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt actggttgcg
     2821 tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac cttgttaatc
     2881 agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt cagtgcctgc
     2941 acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct tccggatgct
     3001 tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg ccggttgccc
     3061 ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct gcgctgaatc
     3121 ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc gcgtcacaca
     3181 gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac ggtgtgtcat
     3241 tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc cagatggtga
     3301 tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta ttcatcttaa
     3361 gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt ggttgcgggc
     3421 gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag ggtgtcggct
     3481 tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg attcagttca
     3541 ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg gtccaggtgg
     3601 tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag gtaatgcagg
     3661 cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca gggcagcccc
     3721 caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt gtctgacacg
     3781 ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa ttctgctcat
     3841 cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat acggaccaaa
     3901 actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct gcacttcacg
     3961 gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga acggaaaaag
     4021 acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga tgattttcat
     4081 gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa ctgaagtgat
     4141 atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa ggagaaaacc
     4201 tggctgccac cgactatcag atggtccggt acccggatat caaccagggc cagtgcctgt
     4261 accagacgct cagtgataag gcggtctgcc ttgctggggg
//
LOCUS       DAJTRN010000190         4217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRN010000190 DAJTRN010000000
VERSION     DAJTRN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..584)
                     /gene="kptA"
                     /locus_tag="OO328_003933"
     CDS             complement(<1..584)
                     /gene="kptA"
                     /locus_tag="OO328_003933"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5932296.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            834..1517
                     /locus_tag="OO328_003934"
     CDS             834..1517
                     /locus_tag="OO328_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5932297.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1514..1975
                     /locus_tag="OO328_003935"
     CDS             1514..1975
                     /locus_tag="OO328_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5932298.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1988..3160
                     /gene="iadA"
                     /locus_tag="OO328_003936"
     CDS             1988..3160
                     /gene="iadA"
                     /locus_tag="OO328_003936"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5932299.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3225..4136
                     /gene="hypT"
                     /locus_tag="OO328_003937"
                     /gene_synonym="qseD"
     CDS             3225..4136
                     /gene="hypT"
                     /locus_tag="OO328_003937"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5932300.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4129..>4217)
                     /gene="iraD"
                     /locus_tag="OO328_003938"
     CDS             complement(4129..>4217)
                     /gene="iraD"
                     /locus_tag="OO328_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="inhibitor of RssB activity in response to DNA
                     damage; blocks degradation of stationary phase sigma
                     factor RpoS; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5932301.1"
                     /translation="LYHDDEPLTFDLVWDNGGWRSATLENVS"
BASE COUNT          956 a         1127 c         1085 g         1049 t
ORIGIN      
        1 tattgttttt gtaccccagt cggcataaac gaccactcaa gaaactcaac agcaaccgtt
       61 gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga tgcctcgttt cgccatttcc
      121 tgtgctttga cggttaagac aaccggcgat ccgtgtcttg ctcccacttt gcgtgccgtt
      181 gcttcatcag cagagagatg cacgtaatga cgatcccctg caatcagccc ctgttttttt
      241 atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa actgtggcgg tgttttttcg
      301 gcaaaggaaa tagcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat gcacctacca
      361 tcactggaat aactaaaacg ctttttatcg ctggttgcga ccacggtatc cagcaatgca
      421 cgggttaatc gcttgcccgc tttttgggca caaagtatga gcttattgat atccgcccat
      481 ccttcacggt ccagtacgat accaatcgcc tccggtttgt gtcggaggac aaaacttaaa
      541 aacttgctcg tatccgccag ctctttttcg ttgtactttg ccatcgcgtt ttcgtccctg
      601 gattttctgt ttttatttac taatgtcatt gtcactgggt ttatgaattt tctgcaactc
      661 aatcctgcgc agaacctttt ccacacagcg taatttttcc cctatgcaaa aaacgcatag
      721 ccagaaacat acttgcattt tccccatacc cacatggctg ataaggtttt tcagttccag
      781 gaattttcct ggctcaagca acacacaaac acaaaaaaac aatggggatt gttatgacac
      841 aacaagggga tgctgtagcc ggggagctgg cgacagaaaa agtgggcatt aaagggtatc
      901 tggctttttt tctcaccata atcttttttc ccggtgtttt ttccggaaca gacagctggt
      961 ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg gcaactgcct ggtgcgaatg
     1021 gagccaccac ctcatttcgc ggggtgggtg gcgcgggggc taaagatggc tttctctttg
     1081 cgctggagct ggcaccgtca gtcattctct ctctggggat tatttcaatt accgatggtc
     1141 ttggcggatt acgcgccgcg cagcagttaa tgacacctgt gctaaaaccg ttgttgggca
     1201 ttccagggat ctgctcgctg gcactgatcg ctaacctgca aaacacggat gccgccgccg
     1261 gtatgaccaa agagctggcg caggaaggtg aaattaccga acgcgataaa gtgattttcg
     1321 ccgcatatca aaccagcggt agcgccatca tcaccaacta cttctcttcc ggcgtcgcgg
     1381 tgtttgcctt tctgggcacg tccgtaatcg tacctttagc cgtcatcctg gtgtttaaat
     1441 ttgtcggagc caacattttg cgcgtctggc ttaacttcga agaacgtcgc aacccaacgc
     1501 aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc atggatatgt ttatcgatgg
     1561 cgcgcgccgt ggctttacta tcgccacgac caacctgctg ccaaacgtgg tgatggcgtt
     1621 tgtcatcatt caggcgctga aaattaccgg cctgctcgac tgggtggggc atatctgtga
     1681 acccgtaatg gcgctgtggg gattacccgg tgaagcggca accgtccttt tagccgcatt
     1741 aatgagtatg ggcggcgcag tcggtgttgc cgccagtctg gcaaccgctg acgcattaac
     1801 cggccacgat gtcaccgttt tactgcccgc aatgtattta atgggcaacc cggtgcaaaa
     1861 tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa tattacccgc atatcattac
     1921 ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg cagcttattg tttaacaagg
     1981 agttaccatg attgattata ccgcagccgg ttttaccctg ctgcagggag cgcatttgta
     2041 tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa ttatcgccgt
     2101 tgccagcaat atcccttctg acattgtacc gaactgcacg gttgtcgatc tcagcgggca
     2161 gatcctctgc ccaggtttta ttgatcaaca cgtccatttg attggcggtg gcggcgaagc
     2221 aggtcccacg acgcgcacgc cggaagtggc gctaagtcgc ctgacggaag cgggcgtcac
     2281 gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc cacccggaat ccctgctcgc
     2341 caagacccgt gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg gcgcttatca
     2401 tgtcccttcc cgcactatta cgggttccgt ggaaaaagac gtggcgatta tcgatcgtgt
     2461 gattggcgtg aaatgcgcca tctctgatca ccgttctgcc gcaccggacg tttatcacct
     2521 ggctaatatg gcggcagaat cccgcgttgg cggtttgctc ggcggtaaac ctggcgtcac
     2581 cgtgttccac atgggcgaca gtaaaaaggc gttaaagcct atttatgacc tgctggaaaa
     2641 ctgcgatgtg ccgatcagca agctgctgcc gacccacgtt aaccgcaacg taccgttgtt
     2701 tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc gatatcacca gcagcattga
     2761 cgaaccggtc gcccctgccg aaggtattgc ccgcgccgtt caggcgggta ttccgctggc
     2821 acgcgtcacc ctcagctccg acggcaacgg tagccagccg ttcttcgatg acgaagggaa
     2881 tttaacccat atcggtgttg caggttttga aacgttgctg gaaaccgtgc aggtgctggt
     2941 caaagactat gatttcagta tcagcgatgc cctgcgcccg ctcaccagta gcgtagccgg
     3001 tttccttaac atgagcggga aaggcgaaat tctgccaggc aatgacgcag acttactggt
     3061 catgacgcca gaattgcgca ttgagcaggt atacgctcgc ggcaaactga tggtcaaaga
     3121 cggcaaagcc tgcgtgaaag gaacgtttga aacggcttaa acggttgtca gccgccctaa
     3181 tggacgtaga atgcccccaa gggcggctga cagagtaaaa cgtaatggat gactgtggtg
     3241 cggttttgca taatattgaa accaaatggc tttatgattt tctgactctg gaaaaatgcc
     3301 gcaatttttc ccaggcggca gtcagtcgca acgtctcgca accggcattc agccgccgca
     3361 tccgtgcact ggaacaggcg attggcgttg aattgtttaa ccgccaggtg acgccactgc
     3421 aactctcaga acaagggaaa atcttccatt cgcagatccg ccatctgttg caacagttag
     3481 aaagcaacct tgcagagctg cgtggcggca gcgattacgc gcaacgtaaa atcaagatag
     3541 ccgctgcaca ctctctttcc ctcggtctgt taccgtccat tatcagccag atgccgccgc
     3601 tctttacctg ggcaattgaa gccattgatg tcgatgaagc ggtcgataaa ctgcgtgaag
     3661 ggcaaagtga ctgtattttt tcctttcacg acgaagattt gctggaagcg ccgtttgacc
     3721 acattcgctt atttgaatct caattgttcc ctgtctgcgc cagtgacgaa cacggagaag
     3781 cactttttaa cctcgcgcag ccacactttc cgttactgaa ttacagccgc aactcctaca
     3841 tggtgcgatt gattaatcgc accctgacgc gccacagtga gttaagtttc agcacctttt
     3901 ttgtctcttc gatgagcgag cttttaaagc aggttgccct cgacggctgt gggattgcct
     3961 ggctgccgga gtatgccatc caacaagaaa ttcgcagcgg gcagctcgtt gtgctgaatc
     4021 gggacgaaca ggtgatcccg attcaggcct acgcataccg gatgaatacc cgcatgaatc
     4081 cagttgccga acgcttctgg cgtgaactgc gcgagctgga gattgtgctt agctgacatt
     4141 ctcaagcgtc gcactgcgcc aaccgccatt atcccagacc agatcaaacg ttagcggttc
     4201 atcgtcgtgg tacagca
//
LOCUS       DAJTRN010000191         4211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTRN010000191 DAJTRN010000000
VERSION     DAJTRN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO328_003939"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO328_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5932302.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO328_003940"
     tRNA            complement(324..399)
                     /locus_tag="OO328_003940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO328_003941"
     tRNA            complement(406..480)
                     /locus_tag="OO328_003941"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO328_003942"
     tRNA            complement(597..681)
                     /locus_tag="OO328_003942"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO328_003943"
     tRNA            complement(690..765)
                     /locus_tag="OO328_003943"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO328_003944"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO328_003944"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5932303.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO328_003945"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO328_003945"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5932304.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO328_003946"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO328_003946"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5932305.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1047 c          937 g         1138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt c
//
LOCUS       DAJTRN010000192         4200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRN010000192 DAJTRN010000000
VERSION     DAJTRN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            84..752
                     /locus_tag="OO328_003947"
     CDS             84..752
                     /locus_tag="OO328_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932306.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO328_003948"
     CDS             complement(995..1690)
                     /locus_tag="OO328_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5932307.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO328_003949"
     CDS             complement(1683..3110)
                     /locus_tag="OO328_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5932308.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO328_003950"
     CDS             complement(3121..3840)
                     /locus_tag="OO328_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5932309.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1120 a         1085 c          869 g         1126 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcggaat
//
LOCUS       DAJTRN010000193         4109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTRN010000193 DAJTRN010000000
VERSION     DAJTRN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..437)
                     /locus_tag="OO328_003951"
     CDS             complement(<1..437)
                     /locus_tag="OO328_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5932310.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRR"
     gene            complement(1044..2309)
                     /gene="efeB"
                     /locus_tag="OO328_003952"
     CDS             complement(1044..2309)
                     /gene="efeB"
                     /locus_tag="OO328_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5932311.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2315..3442)
                     /gene="efeO"
                     /locus_tag="OO328_003953"
     CDS             complement(2315..3442)
                     /gene="efeO"
                     /locus_tag="OO328_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5932312.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3500..>4109)
                     /locus_tag="OO328_003954"
     CDS             complement(3500..>4109)
                     /locus_tag="OO328_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5932313.1"
                     /translation="VAVIAVVILTWMVFWMRKVSRNVKVQLEQAVDSALQRGNHHGWA
                     LVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLATAVVLGFLLYWGGIRLN
                     LGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFDMSAVLSTHSLFGTLME
                     GIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA"
BASE COUNT          957 a         1151 c         1002 g          999 t
ORIGIN      
        1 aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt ttctgcgata
       61 tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt gacgataatc
      121 ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat ccaggttttt
      181 ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc ctgcagatag
      241 tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata
      301 ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc ttcacgctgc
      361 ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc tttgatcact
      421 gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca cgcgccgttg
      481 gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc atagtttgct
      541 tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt aacacgagta
      601 aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag aaatctccgc
      661 gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc atccgcgatc
      721 tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa gtacaacaga
      781 actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc atttcggtga
      841 cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag ctgtgtgctt
      901 tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc ggcgcgaacg
      961 ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc gataaacctg
     1021 cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag ataatcgttc
     1081 gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt aacgtattcc
     1141 tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc cagatcgtgt
     1201 tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac ccccaacgaa
     1261 taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg gggattcgcc
     1321 agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc gtaatcaggc
     1381 acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg gccaaaaatc
     1441 gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg aatcaagcgt
     1501 actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc cgttacccac
     1561 accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc gtctttgaaa
     1621 cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc ggaaataaac
     1681 ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat atcgcgcagc
     1741 gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc accatgacat
     1801 aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt ttttggcatc
     1861 tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa cgtgatggtg
     1921 agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg caggcgcgga
     1981 tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg agtcaacaag
     2041 cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc caccagcatc
     2101 attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa cggctgtttc
     2161 tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg tgcatgagcg
     2221 accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg tgacggttca
     2281 ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc acaccgcgaa
     2341 gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc cggtcggcat
     2401 cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc agaatggtat
     2461 cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct ttttgcagtt
     2521 gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg aaatcccaca
     2581 gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc acttcctcaa
     2641 tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca ctgatgcgtt
     2701 tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc cctttggtgg
     2761 tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt gggtcggcgg
     2821 ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg gagaacagtt
     2881 cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt gctttcgctt
     2941 tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc accagctgcg
     3001 tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga cttaacagcg
     3061 catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc cctttcgggt
     3121 tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc gtcattttct
     3181 ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct ttgaggatct
     3241 cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc ccggcgttaa
     3301 ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc tgcggcacat
     3361 cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac tgcaatgcgt
     3421 tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg taactaagca
     3481 agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg cgtggtggca
     3541 gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact tcgctgacgc
     3601 tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc gagtgagttg
     3661 agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat ccggcttcat
     3721 gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata aacaggctgg
     3781 tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc aggaagccga
     3841 gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc ccgacatctt
     3901 gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca acggcaaaaa
     3961 agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg ctatcgactg
     4021 cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa accatccagg
     4081 taaggatcac cacggcgatc accgccacg
//
LOCUS       DAJTRN010000194         4002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTRN010000194 DAJTRN010000000
VERSION     DAJTRN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..320
                     /locus_tag="OO328_003955"
     CDS             <1..320
                     /locus_tag="OO328_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5932314.1"
                     /translation="SGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKL
                     AAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAE
                     SAH"
     gene            342..2333
                     /gene="cyoB"
                     /locus_tag="OO328_003956"
                     /pseudo
     CDS             342..2333
                     /gene="cyoB"
                     /locus_tag="OO328_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2323..2937
                     /locus_tag="OO328_003957"
     CDS             2323..2937
                     /locus_tag="OO328_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5932315.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            2937..3266
                     /locus_tag="OO328_003958"
     CDS             2937..3266
                     /locus_tag="OO328_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5932316.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3278..>4002
                     /gene="cyoE"
                     /locus_tag="OO328_003959"
     CDS             3278..>4002
                     /gene="cyoE"
                     /locus_tag="OO328_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5932317.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSV"
BASE COUNT          819 a         1015 c         1098 g         1070 t
ORIGIN      
        1 acagcgggcc gggcttctca ggcatgaagt tcaaagctat tgcaacaccg gatcgcgccg
       61 aattcgacca atgggtcgca aaagcgaaac agtcgccgaa caccatgtct gacatggctg
      121 cgttcgaaaa actggccgcg cctagcgaat acaaccaggt ggaatatttc tccaacgtga
      181 aaccagactt gtttgccgat gtgattaaca agtttatggc tcacggtaag agcatggaca
      241 tgacccagcc agaaggtgag cacagtgcac acgaaggtat ggaaggcatg gacatgagcc
      301 acgcggaatc cgcccattaa aggggttgag gaagaataaa gatgttcgga aaattatcac
      361 ttgatgcagt cccgttccat gaacctatcg tcatggttac gatcgctggc attattttgg
      421 gaggtctggc gctcgttggc ctgatcactt acttcggtaa gtggacctac ctgtggaaag
      481 agtggctgac ctccgtcgac cataaacgcc tcggtatcat gtatatcatc gtggcgattg
      541 tgatgttgct gcgtggtttt gctgacgcca ttatgatgcg tagccagcag gctcttgcct
      601 cggcgggtga agcgggcttc ctgccacctc accactacga tcagatcttc accgcgcacg
      661 gcgtgattat gatcttcttc gtggcgatgc ctttcgttat cggtctgatg aacctggtgg
      721 ttccgctgca gatcggcgcg cgtgacgttg cgttcccgtt cctcaacaac ttaagcttct
      781 ggtttactgt tgttggcgtg attctggtta acgtttctct cggcgtgggc gaatttgcgc
      841 agaccggctg gctggcctat ccaccgctat cgggaataga gtacagtccg ggagtcggtg
      901 ttgattactg gatatggagt ctccagctat ccggtatagg tacgacgctt accggtatca
      961 acttcttcgt taccattctg aagatgcgcg caccgggcat gaccatgttc aagataccag
     1021 tatttaccta ggcatcactg tgcgcgaacg tactgattat tgcttccttc ccaattctga
     1081 cggttaccgt cgcgttgttg accctggatc gctatctggg cacccatttc tttaccaacg
     1141 atatgggtgg caacatgatg atgtacatca acctgatttg ggcctggggc cacccggaag
     1201 tttacatcct gatcctgcct gttttcggtg tgttctccga aattgcggca accttctcgc
     1261 gtaaacgtct gtttggttat acctcgctgg tatgggcaac cgtctgcatc accgtgctgt
     1321 cgttcatcgt ttggctgcac cacttcttta cgatgggtgc gggcgcgaac gtaaacgcct
     1381 tctttggtat caccacaatg attatcgcca tcccgaccgg ggtgaagatc ttcaactggc
     1441 tgttcaccat gtatcagggc cgcatcgtgt tccattctgc gatgctgtgg actatcggtt
     1501 ttatcgtcac cttctcggtg ggcggtatga ctggcgtgct gctggcagta ccgggcgcgg
     1561 acttcgttct gcataacagc ctgttcctga ttgcgcactt ccataacgtg atcatcggcg
     1621 gcgtggtctt cggctgcttc gcagggatga cctactggtg gcctaaagcg ttcggtttca
     1681 aactgaacga aacctggggt aaacgcgcgt tctggttctg gatcatcggc ttcttcgttg
     1741 cctttatgcc gctgtatgcg ctgggcttca tgggcatgac ccgtcgtttg agccagcaga
     1801 ttgacccgca gttccacacc atgctgatga ttgcagccag cggtgcagta ctgattgcgc
     1861 tgggtattct ctgcctcgtt attcagatgt acgtttctat tcgcgaccgc gaccagaacc
     1921 gtgacctgac tggcgacccg tggggtggcc gtacgctgga gtgggcaacc tcttccccgc
     1981 ctccgttcta taactttgcc gtagtgccgc acgttcacga acgtgatgca ttctgggaaa
     2041 tgaaagagaa aggcgaagcg tataaaaagc ctgaccacta tgaagaaatt catatgccga
     2101 aaaacagcgg tgcaggtatc gtcattgcag ctttctccac catcttcggt ttcgccatga
     2161 tctggcatat ctggtggctg gcgattgttg gcttcgcagg catgatcatc acctggatcg
     2221 tgaaaagctt cgacgaggac gtggattact acgtgccggt ggcagaaatc gaaaaactgg
     2281 aaaaccagca tttcgatgag attactaagg cagggctgaa aaatggcaac tgatactttg
     2341 acgcacgcga ctgcccacgc gcacgaacac gggcaccacg atgcaggcgg aaccaaaatc
     2401 ttcggatttt ggatctacct gatgagcgac tgcattctgt tctcaatctt gtttgctacc
     2461 tatgccgttc tggtgaacgg caccgcaggc ggcccgacag gtaaggacat tttcgaactg
     2521 ccgttcgttc tggttgaaac tttcttgctg ttgttcagct ccatcaccta cggcatggcg
     2581 gctatcgcca tgtacaaaaa caacaaaagc caggttatct cctggctggc gttgacctgg
     2641 ttgtttggtg ccggatttat cgggatggaa atctatgaat tccatcacct gattgttaac
     2701 ggcatggggc cggatcgcag cggcttcctg tcagcgttct ttgcgctggt cggcacgcac
     2761 ggtctgcacg tcacttctgg tcttatctgg atggcggtgc tgatggtgca aatcgcccgt
     2821 cgcggcctga ccagcactaa ccgtacccgc atcatgtgcc tgagcctgtt ctggcacttc
     2881 ctggatgtgg tttggatctg tgtgttcact gttgtttatc tgatgggggc gatgtaatga
     2941 gtcattctac cgatcacagc ggcgcgtccc atggcagcgt aaaaacctac atgacaggct
     3001 ttatcctgtc gatcattctg acggtgattc cgttctggat ggtgatgaca ggggctgcct
     3061 ctccggccgt aattctggga acaatcctgg caatggcagt ggtacaggtt ctggtgcatc
     3121 tggtgtgctt cctgcacatg aataccaaat cagatgaagg ctggaacatg acggcgtttg
     3181 tcttcaccgt gctaatcatc gctatcctgg ttgtaggctc catctggatt atgtggaacc
     3241 tcaactacaa catgatgatg cactaagagc ggcggttatg atgtttaagc aatacctgca
     3301 agtaacgaaa ccaggcatca tctttggcaa cctgatctcg gtgattgggg gattcctgct
     3361 ggcctcaaag ggcagcattg attatcccct gtttatctac acgctggttg gggtgtcact
     3421 ggttgtggcg tcgggttgtg tgtttaacaa ctacatcgac agggatatcg acagaaagat
     3481 ggaaaggacg aagaatcggg tgctggttaa aggcctgatc tctcctgctg tctcgctggt
     3541 gtacgccacc ttgctgggta ttgctggctt tatgctgctg tggtttggcg cgaatccgct
     3601 ggcctgctgg ctgggggtga tgggctttgt ggtttatgtc ggcgtctata gcctgtacat
     3661 gaaacgccac tcagtctacg gcacattgat tggttcgctc tccggcgctg cgccgtcggt
     3721 gattggctac tgtgcagtaa ccggcgagtt tgatagcggc gcagcgatcc tgctggctat
     3781 cttcagcctg tggcagatgc ctcactccta tgccatcgcc attttccgct ttaaggatta
     3841 ccaggcggca aacattccgg tattgccagt ggtaaaaggc atttcggtgg cgaagaatca
     3901 catcacgctg tatatcatcg cctttgccgt tgccacgctg atgctctctc ttggcggtta
     3961 cgctgggtat aaatatctgg tggtcgccgc ggcggttagc gt
//
LOCUS       DAJTRN010000195         3929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRN010000195 DAJTRN010000000
VERSION     DAJTRN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(580..771)
                     /locus_tag="OO328_003960"
     CDS             complement(580..771)
                     /locus_tag="OO328_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5932318.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            836..1159
                     /locus_tag="OO328_003961"
     CDS             836..1159
                     /locus_tag="OO328_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5932319.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1101..1859
                     /locus_tag="OO328_003962"
                     /pseudo
     CDS             1101..1859
                     /locus_tag="OO328_003962"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1837..2367
                     /locus_tag="OO328_003963"
     CDS             1837..2367
                     /locus_tag="OO328_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5932320.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2374..2607
                     /locus_tag="OO328_003964"
     CDS             2374..2607
                     /locus_tag="OO328_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5932321.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3153..3545)
                     /locus_tag="OO328_003965"
     CDS             complement(3153..3545)
                     /locus_tag="OO328_003965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932322.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          943 a          891 c         1165 g          930 t
ORIGIN      
        1 acctacaccg aactgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc ggtttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccatc
      241 ggcgcggcct tcttcctgtt cctggtcttt tgctcacatg ttctttccgg cgttatcccc
      301 tgattctgtg gataacagca tttcgttttt gagtgagtag ataccgctcg ccgcagtcga
      361 acgaccgagc gtagcgagtc agtgagcgag gaagcggaaa agcgcctgga cgtgcatttt
      421 ctccttacgc atctgtgcgg catttcacac ccggtatggc gcacttttca tacaatccgc
      481 actgatgccg catggttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
      541 ctgcgccccg acacccgcta acacccgctg acaagcccgt cagaggtttt caccgtcatc
      601 cccgaagcga gcgaggcaac tgcggtaaag ctcatcggcg tggtcgtaaa gcgattcaca
      661 gatgtctgcc tgttcatccg cgtccagttc gttgagcttc tccagcagcg ttaatgtctg
      721 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
      781 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgcg agaggatgct
      841 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgctgtg agggtaaaca
      901 gctggcggta tggatgcggc gggtctgcct gggtgagccg gttgcccgtt ccgggaggct
      961 gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcgggaata acctgaatca
     1021 ggcagcgagg aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc
     1081 cgcactgatg gccatcgggg atgagctgcg ccggctgcgt ctggccgtca gggaacaggg
     1141 gacgcgggat gatagttaag tttcatgcca ggggaaaagg cggtggcagt ggtccggttg
     1201 attacctgct gggaagggag cgtaaccggg aaggggcaac ggtgcttcag ggtaatccgg
     1261 aagaagtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc
     1321 tctcgttcgc ggagaaggag ctgccgccgg gagggcgtga aaaagtgatg gcgagctttg
     1381 agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag cattctgtgg gtggagcacc
     1441 aggacaaggg gcggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa
     1501 aacgcctcca gccgtactac gaccgcgcag acaggccgag aattgatgcc tggcagacgc
     1561 tggtgaatca ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc cggacgctga
     1621 ctctccctga taacctgccg gaaacgaaac aggcgcttgc tgagagcatt acacggtgga
     1681 tagacgctct ttatcatgtc ggggagataa aaggccgtca ggatgtgatt caggcgctca
     1741 cagaggcggg gcttgaagtg gtcagggtga cgcgaagcag tatcagcatt gcagacccga
     1801 acggcgggaa gaatatcaga ctgaaaggag cattctatga gcaatctttt acagacgggc
     1861 gcggagttcg agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgagcgac
     1921 gagttcagga agctcgaaga atctgtaagc aaggctgtga catcaaacga gacgaaaatc
     1981 aaagacgcta tagccctgtt cacagcttcg acagaggaat cactgaaaaa acatcgggaa
     2041 ggggtgaaag aggcgatgat gcagcacagg aaggacgtgt taaagctggc agggaatacg
     2101 ggcatgatgt tactggggat ggtcattttc ctgtttaccg tgagtggcgg gacgctctgg
     2161 tatcttggcg ggatgataca ggcgaacctg gaagaaatca ggaagcagaa cgagagtctg
     2221 gagaagctga acgcgaagac atggggcgtg cgttatcacg aggggagcaa cggacgtttt
     2281 ctggtgttgc ccgaggggat gaaagccgaa acgaactgga cgatggaaaa cgggaaacag
     2341 aatgccgtaa ggctggtaca ggagtgacag agcatgacgg agctggaaag ccatttgctg
     2401 aacgcattag agcagctgca acaggactat atgcagcggc tgaacgaatg ggagagcgcc
     2461 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
     2521 cgggtcatgc agttgagtca gcaggtgctg cacttgagcg agcagacaga acgcttgagc
     2581 cagttataca gcgagaactg gagataagag aggaacggtt gatacaggag cgcgaacatg
     2641 cgttattcct ggaacgggaa cgccagccgg aaatacagga acggacactg gatggccctt
     2701 cgctgggatg gtaatcagag acaggctaaa atgttgtcag cgcgtttccg gtgcattttt
     2761 tgggataatg gttggttcgg gggcaccggg aacgcgcttc agtcagacct tgctgaaaac
     2821 acaggaacga gttaaaacgc atacgggcgc gattctggtg tgtcttttgt cgctgtgttt
     2881 tgcctctatt gccagatcgc gctcattccc ctgaacttct gttttcccgc tcaggtcccc
     2941 gtcttcttcg cttccgaaag catgaataaa atccgtttca gctgtctctg gctggcaatt
     3001 cctgcgtgat tcacatggct gcatagctat gcatgtggtt aaaatttacc gggtgcgatc
     3061 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgccg
     3121 aacgcgtttc agcggtgcgt acaattaaga tattacgtta aattgtgagg gaatggtccc
     3181 catatactga ctttatcata gggatagggt tggtagtggg tcgattgggt ttcggcattg
     3241 tttccgttaa tgccaattgc atttttgtat ttgatgcaac agaaggcgtg agattctcct
     3301 gggcctggaa aggctaagtg tttagaattg atggcaaaat atcttccgtc cgatttttga
     3361 agtagttgtt gtttggctgt ctcccaaagg gtgctgttac aaacttcttg aatttgtagt
     3421 ttttgcagga ctcttattac tgcgttgtca tgttccaatt ctttagggga gtgtattatt
     3481 ctctctttta ataagctgtt taccacaggg tttagttgtt gtctaaagca cgcagataat
     3541 gccattgcta cacaattttg ttcccgagga agcatgtttg gcggggtatg ccgtgtttga
     3601 tgtgatatgt ctattctcat agaaggtctc tggcttatag catcaacaaa tacggctata
     3661 aaacaaaatt ttcatagagt gattcaagat cttcttgaga tccttttttg cggcgcgtaa
     3721 tctgctgcct gcaaacaaaa aaaccaccgc taccaacggt ggtttgtttg ccggaatcta
     3781 gagctaccaa ctctttcttc cgaaggtaac tggcttggtg gagcacagat accaaatgct
     3841 gtccttctag tgtagccgca gttaggccac cacttcaaga actcttaata tctcaatcca
     3901 ccttgtccag ttaccaatgg ctgctgcca
//
LOCUS       DAJTRN010000196         3878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRN010000196 DAJTRN010000000
VERSION     DAJTRN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..238
                     /gene="tauB"
                     /locus_tag="OO328_003966"
     CDS             <1..238
                     /gene="tauB"
                     /locus_tag="OO328_003966"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Part of the ABC transporter complex tauABC involved
                     in taurine import; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5932323.1"
                     /translation="TGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFARRF
                     VAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            235..1062
                     /gene="tauC"
                     /locus_tag="OO328_003967"
     CDS             235..1062
                     /gene="tauC"
                     /locus_tag="OO328_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5932324.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            1059..1910
                     /gene="tauD"
                     /locus_tag="OO328_003968"
     CDS             1059..1910
                     /gene="tauD"
                     /locus_tag="OO328_003968"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5932325.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2016..2990)
                     /gene="hemB"
                     /locus_tag="OO328_003969"
     CDS             complement(2016..2990)
                     /gene="hemB"
                     /locus_tag="OO328_003969"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5932326.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            3514..>3878
                     /locus_tag="OO328_003970"
     CDS             3514..>3878
                     /locus_tag="OO328_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5932327.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTT"
BASE COUNT          889 a          964 c         1030 g          995 t
ORIGIN      
        1 gacgggcaag caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac
       61 tgaactggtt ctgctttcat ccggccctgg ccgtgtgctg gagcggctgc ggctcaactt
      121 tgctcgccgc tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat
      181 cgccatgcgc gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt
      241 gtgctcatta atgaaaaact gcattcgcgg cggctgaaat ggcgctggcc gctctcgcgt
      301 caggtgacct taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg
      361 gcgctgcaac tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg
      421 ctcaccattg ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc
      481 agcctgacgc gcattgtgct ggcgctactc gcggcggtgg tgatcggtat tccggtcggg
      541 atcgccatgg ggctaagccc gacggtgcgc ggcattctgg atccggtaat cgaactttat
      601 cgtccggtgc caccgctggc ttatttgccg ctgatggtga tctggtttgg tattggtgaa
      661 acctcgaaga tcttactgat ctatttagcg atttttgcgc cggtggcgat gtcggcgctg
      721 gcaggggtga aaagcgcgca gcaggttcgc attcgtgccg cccagtcgct gggtgccagc
      781 cgtgcgcagg tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga
      841 ttacgcattg gtctgggtgt gggctggtct acgctggtag cggcggagct gattgccgcg
      901 acacgcggtt taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggta
      961 ctggcgggga tcgcggtgat tgcgattatc gcctttcttt tagaactggg tctgcgcgcg
     1021 ttacagcgcc gtctgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta
     1081 ccccgctggg gccgtatatc ggcgcacaaa tttcgggtgc cgacctgacg cgcccgttaa
     1141 gcgataatca gtttgaacag ctttaccatg cggtgctgcg ccatcaggtg gtatttctgc
     1201 gcgatcaagc tattacgccg cagcagcaac gcgcgctggc ccagcgtttt ggcgaactgc
     1261 atattcaccc tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc
     1321 ataacgataa tccgccggat aacgacaact ggcataccga tgtgacattt attgaaacgc
     1381 cacccgcagg ggcgattctg gcagctaaag agttaccttc gaccggcggt gatacgctct
     1441 ggaccagcgg tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagct
     1501 ggctgcgtgc ggagcatgat ttccgtaaat cattcccgga atacaaatac cgcaaaaccg
     1561 aggaggaaca tcaacgctgg cgcgaggcgg tcgcgaaaaa tcctccgttg ctacatccgg
     1621 tggtgcgaac gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta
     1681 cgcgaattgt cgatgtcagc gagaaagaga gcgaagcctt gttaggcttt ttattcgccc
     1741 atatcactaa accggagttt caggtgcgtt ggcgctggca accgaatgat attgcgattt
     1801 gggataaccg cgtgactcag cactatgcca atgccgatta cctgccacag cggcggatta
     1861 tgcatcgggc gacgatcctt ggggataaac cgttttatcg ggcggggtga tacgagtgcg
     1921 gacggttccc tcgccccctt ggggagaggg ttagggtgag ggggcgttca ctgtactttc
     1981 aacaggttaa ctcccccttt ctgagaggaa aaaaattaac gcagaatctt cttctcagcc
     2041 aaatccagcg caaagtagct gaaaatcaga tccgcacccg cacgcttaat tgaacctaag
     2101 ctttcgagca cgactttctc ttcatctata gcacccgcca gcgcggcgaa cttaatcatc
     2161 gcgtactcac cgctcacctg atacgcgcca atcggcaatt cagtacgttc acgcagctca
     2221 cgcacgatgt cgaggtacgc tccagcaggt ttaaccatca ggcagtctgc gccctgggct
     2281 tcatccagca gtgactcacg aatcgcctcg cgacggttca ttgggttcat ctgatagctt
     2341 ttgcggtcgc cttttaatgc gcttcctgca gcttcacgga acgggccata aaaggaagag
     2401 gcgaacttgg tcgaatacga cataatcgcc gtatctttaa agcctgcagc gtccagcgcc
     2461 tgacgaatcg cctgtacctg gccgtccatc gcggcggaag gggcgatgaa gtctgcgcct
     2521 gcagcagctg caaccacggc ttgcttgcct aaattttcca gagtcgcgtc gttgtcgacg
     2581 ccatgctcgc acagcacacc gcagtgaccg tgagaagtgt attcacagaa acaagtgtct
     2641 gacatgacga tcatttctgg cacagtctgc ttgcagatgc gcgacatacg cgccaccagt
     2701 ccatcttccc gccaggcgtc gctgccggtt tcatcggtat ggtgagagat gccaaaagtc
     2761 attacggaac ggataccggc gttggcgatg cgttcaattt cgcgtgccag atgtttctct
     2821 ggaatgcgca tcacgcctgg catggcttca acggctttgt agtcgtcaat ttcttcttca
     2881 acaaagatcg gcaacaccag gtcgttaagg ctaagtgttg tctcttcaaa catagcgcgc
     2941 agcgcaggag atttgcgcag gcgacgaggg cgttggatta agtctgtcat ggtctgcctg
     3001 atgtttgtgg aattaaggtg cacagtatac ctgaagcggg gcaggagtgt tttacgaaag
     3061 ttgtcgcgat gttgacaaga agagaatgaa agagagacca ggacatcttt gtaatagaaa
     3121 tagcaataat ttcatattca ctgaaaatat ttttaatctt tatttatagc tcttggttat
     3181 tattttttgg cgtttggttg tgctgctata aattgaataa ttcaaacttt gttgtatatt
     3241 gataagatat ttatctggtt tgtatttttg atatttataa cgcgtatgca tcaaaaataa
     3301 cattatctgc aacatactga aatatcgtcc ttttatcaat ttgatgcgtg tttttctcca
     3361 taaattatat atgtccacat tcggacttag gggaaagaat aattgaacca ttcgtctgta
     3421 acgcagcata atcgttagcg cgaaacataa tatgttttct atgcattgat aattgatgga
     3481 tcaacttatt acgtccctga ggagggatga caaatgcact cctggaaaaa gaaacttgta
     3541 gtatcacaat tagcattggc ttgcactctg gctatcacct ctcaggctaa tgcagcgacc
     3601 aacgatattt ctggtcaaac ttacaatact ttccatcact acaacgacgc cacctatgct
     3661 gatgacgttt actatgatgg ttatgtaggc tggaacaact atgccgctga tagctattac
     3721 aacggcgata tctacccggt cattaataac gctaccgtta acggcgtgat ttctacctac
     3781 tatctggacg acggtatttc taccaatacc aacgccaata gtctgacaat caaaaacagc
     3841 actattcacg gtatgattta ttccgagtgc atgacaac
//
LOCUS       DAJTRN010000197         3798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTRN010000197 DAJTRN010000000
VERSION     DAJTRN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(118..864)
                     /gene="ydfG"
                     /locus_tag="OO328_003971"
     CDS             complement(118..864)
                     /gene="ydfG"
                     /locus_tag="OO328_003971"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5932328.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            1001..3046
                     /gene="dcp"
                     /locus_tag="OO328_003972"
     CDS             1001..3046
                     /gene="dcp"
                     /locus_tag="OO328_003972"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5932329.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(3091..3609)
                     /locus_tag="OO328_003973"
     CDS             complement(3091..3609)
                     /locus_tag="OO328_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5932330.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT          946 a          923 c          940 g          989 t
ORIGIN      
        1 gtgggattaa gttgcgtttc gacggtcatt cgttcttgct tgtgagtgag ttaactgcat
       61 gagtctacca cttttttgtg acaagcaata acccggcagt tacgccgggt ataaaaatta
      121 ctgacggtgg acattcagtc cggcatagct ttgggtaacc ggcatcattt ccagggtatt
      181 gatattgacg tgagcaggca gcgttgacac ccaccagacg gcttcgctga catcttctgg
      241 cgtcaatgca acggtatttt gataggtttt ttccgcttta ccgtcatcgc ctttaaagcg
      301 gacattggaa aactcggtgc cacccaccag acccggttcg atgtcggtga cgcgcaccgc
      361 cgtaccatgc agatccgtac gcagattcag gctaaactga cgaacaaacg ctttcgtcgc
      421 accgtaaacg ttaccaccgg catacggcca gctgcctgcc gttgagccaa tgttaataat
      481 atgaccatga ttacgttcaa ccatacccgg taagacggcg cgcgtcatat atactaggcc
      541 tttgttgttg gtatcaatca tcgtttccca gtcttcaacg ctggctttat gcgcaggctc
      601 catgcccaac gccaggccgg cattatttac caggatatca atattgctcc actcggcagg
      661 gagcgatgcc agcatctctt caatagcggc gcggttgcga acgtccagtt gggcgatata
      721 cagattatct cccagttcgt ctgttaactc ctgcaaccgc tcctggcgac ggccagtggc
      781 gataacttta tgcccttgtt gaataaaacg acgagtaatg cattcaccaa aacctgccgt
      841 tgctccagtt actaaaacga tcatctcact gttcctcaac gcttttgtgt gtcaataaca
      901 tagcacgtga tgcttaagag cgggtaatac tggtttgcaa gattgcactc cgttatcagt
      961 aagcctacgc tatggaacac ctcgcgttca ggagaagaaa atgacaacaa tgaatccttt
     1021 ccttgtgcaa agcacactgc cgtatctggc tcctcatttt gatcaaattg ccaatcatca
     1081 ctatcgcccg gcattcgatg agggaatgca acaaaagcgg gcagaaattg ctgccatcgc
     1141 gcttaacccg caaacgcctg atttcaacaa tactattctg gcactggaac aaagcggaga
     1201 attaattacc cgcgttacca gcgtcttttt tgcgatgact gcggcgcata ccaatgatga
     1261 attacagcgt cttgatgaac agttttccgc tgaactggcg gaactggcta atgatatcta
     1321 tctgaacggt gaattattcg cgcgggtaga tgctgtctgg cagcgccgtg aatccctggg
     1381 gcttgatagt gaatccatcc gcctggtgga ggttattcat caacgttttg tccttgccgg
     1441 agccaaactt gcacaagctg ataaagcaaa attaaaagta ctgaatacag aagctgcgac
     1501 cctgaccagc cagtttaacc agcggttact tgcagcaaat aaatccggcg gtctggttgt
     1561 gaacgatatc gcgcagctgg caggaatgag tgagcaagag attgcgctgg cggcagaggc
     1621 ggctcgcgag aaaggtctgg ataacaaatg gctgattccg ctgctgaata ccacccaaca
     1681 accggcgctt gccgaaatgc gcgatcgtgc gacgcgtgaa aaactgttta ttgcgggctg
     1741 gacgcgagcg gaaaaaaatg atgccaatga tacccgcgct atcattcaac gtctggtaga
     1801 gattcgcgca cagcaggcga aactgcttgg ttttcctcat tatgccgcat ggaaaatcgc
     1861 cgatcagatg gcaaaaacgc cagaagcagc actcaacttt atgcgggaaa ttgttccagc
     1921 ggcgcgtcaa cgtgcgagcg atgaattagc ctccatacag gcggttatcg ataagcagca
     1981 gggcgggttt agcgcgcagc cgtgggactg ggcattttat gcggaacagg tacggcggga
     2041 gaaatttgat cttgatgagg cgcagctcaa gccatatttt gaattaaaca cggtgttaaa
     2101 tgaaggtgta ttctggaccg cgaatcagct cttcggtatt aagtttgtcg aacgttttga
     2161 tattccggtc taccatcctg acgttcgtgt gtgggaaatt tttgatcata atggcgtggg
     2221 actggcgtta ttttacggtg atttcttcgc ccgtgattca aaaagcggcg gtgcatggat
     2281 gggcaatttt gttgagcaat caacgcttaa tgaaacgcat ccggtaattt ataacgtctg
     2341 caattatcag aaacccgctg ccggtgagcc tgcgttgtta ctctgggatg atgtaataac
     2401 cttattccat gaatttggtc atacgctgca cggccttttt gcccgccagc gttatgccac
     2461 gctttccggc accaacacgc cgcgtgattt tgtcgaattt ccgtcgcaaa tcaacgaaca
     2521 ctgggcaacg catccgcagg tattcgctcg ctacgcccgg cattatcaga gcggggcagc
     2581 aatgcctgac gaactgcaac agaaaatgcg taatgccagc ctgttcaaca aagggtatga
     2641 gatgagcgaa ctgcttagcg ccgcacttct cgatatgcgc tggcattgcc tggaagaaaa
     2701 cgaagcaatg caggatgtcg atgattttga attgcgggcg ctggtggcgg aaaatatgga
     2761 tcttcctgct ataccgccac gctatcgcag cagttatttc gcccatattt ttggtggcgg
     2821 atatgctgca ggttattacg cttatctgtg gacgcaaatg ttggccgatg atggttacca
     2881 gtggtttgtt gagcagggcg gattaacgcg tgaaaatggg cagcgttttc gcgaggcgat
     2941 cctttccaga ggtaacagcg aagatctgga acgcctgcat cgacaatggc gtggtaaggc
     3001 tcctcagatt atgccgatgc tgcaacatcg tggcttgaac atataaacac caccaatggc
     3061 tggcctggag atatcgccag gccagcctgg ttatagcagt aattgcagca gctgggcgag
     3121 ggcaaacctc accgctaaag ctaataccgt ttcgcagtcg ccagcaaaat gcataaccgc
     3181 agtgtagttc tggcctttaa tatgccacgc gaaccagacg gtacccgctg gcgtaccatc
     3241 ttcaccgccc tccggtccgc cgtagccggt aatggcaata ctgacatcag catccgcacg
     3301 ctctatggca cctgttgcca tttctgctgc cactttctca ctcaccgcag aatatcgttc
     3361 aagagactgc tggcttacgc tgaggatttt catctttgcc tgatcggtga aagtaacaaa
     3421 gcctgcaccg taaaatttgg gtgtatcttc agctgcacac agggcgctag ccaacttacc
     3481 gccggtgcat gattcggcgg tagttaagcg taattgatcc gcgataagtc tttgactcaa
     3541 cgcggatgtc aggttttcaa tagtgtcggt atctgcgagt tgtacaattt tatctttatt
     3601 aatgttcact gttttttgtc cacgctatta tatgtaaagg tgatttgctt tttctgtatg
     3661 ctcgtccaat ataataattt tcaaacatat aaatccgatt attctgaaaa catgtttaag
     3721 actcttcaat cttcttaact ttagtgagtc aaattctctg aatatttgac agctagtggg
     3781 ctgattttaa atgcctta
//
LOCUS       DAJTRN010000198         3693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTRN010000198 DAJTRN010000000
VERSION     DAJTRN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            324..1976
                     /locus_tag="OO328_003974"
     CDS             324..1976
                     /locus_tag="OO328_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5932331.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(1987..2157)
                     /locus_tag="OO328_003975"
                     /pseudo
     CDS             complement(1987..2157)
                     /locus_tag="OO328_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2290..2409)
                     /locus_tag="OO328_003976"
                     /pseudo
     CDS             complement(2290..2409)
                     /locus_tag="OO328_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2483..3413)
                     /locus_tag="OO328_003977"
                     /pseudo
     CDS             complement(2483..3413)
                     /locus_tag="OO328_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1016 a          852 c          847 g          978 t
ORIGIN      
        1 tctccaggaa acttggggcg gttcacgtca gtatcagacc atattaattg ctgaaactga
       61 taaacatcaa accttataga agggtgaatg ctgaaagggg gctatggtga tcgacgtgat
      121 gacaacgaca ggtaagaccg tgacctgata aacctgaatt gtgtcctaga cataaagtcc
      181 gtgaagggaa taaggctcag gtcagcgaat cttatagtgg cccgcagctc tgactgcaat
      241 aaggccggat acagagcagc aactactcgt cgatgtcaga acaacggaag ccttgcaaac
      301 ggggatgcgt tcatacagga acaatgatac cgttaaaaaa aaacattacg ctaataatgt
      361 tcaccctgtc attactgaca ggaaatcctg ctatagcgta tgaaacagat aaagtatata
      421 aaataaccgt tctgcataca aatgaccacc atggacactt ttggcgtaac aatcatggtg
      481 agtacgggtt atcctcccaa aaaactctgg tggacaatat ccggcagaaa gtaataaata
      541 atggcggaag tgttctatta ttatctggtg gtgatattaa caccggtgta cctgaatcag
      601 accttcagaa ggctgaaccc gatattcgtg gaatgaatct aattggttat gatgcaatgg
      661 ccgtcggtaa tcatgaattt gataatcctc taaatatatt acgtcagcag gaaaaatggg
      721 ccacatttcc tttcctttct gctaacatat atcaaaaaag tacagggagg cgtttattca
      781 gtccatggaa aattttcatc cgtcagaatt tgaaaattgc agtcattggc ttgacgacag
      841 atgatactgc gaaaacagga aattctgaat attttactga tattgagttc cgtcaacctg
      901 ctgctgaggc gaggagtgtc attgatgaac ttaatcagca ggaaaagcca gatattatta
      961 ttgcggcaac acatatgggt cactatgaca atggcgagtc aggttcgaat gctcctggtg
     1021 atgttgaaat ggctcgcagt ttgccgactg gctcattagc aatgattgtg ggaggacatt
     1081 cacaagctcc tgtctgcatg gccagcgata ataaaaaaca gtggaattat attccgggga
     1141 caacctgtgt accagataaa caaaatggca tctggattgt acaagcgcat gagtggggaa
     1201 aatatgtcgg tcaggctgat tttgaattct gtaacggtac aatgaaactg gttaattatc
     1261 agttacaccc agttaatctc aaaatgagaa ttaccagaga agatggaaaa acggaatttt
     1321 ctttttacac ccccgaaatt actgaagatc cacagatgtt atccttactg acaccattcc
     1381 agaataaagg caaagcacaa ctggatgtga aggttggggt tgtgaacgga cgtctggaag
     1441 gggacagaag taaagttcgt tttgttcaga ccagtatggg ccatctgatt ctgagcgctc
     1501 tgacagaacg tattgacgca gattttgctg ttgtcagtgg tggggaaatt cgagattcca
     1561 tagagagtgg taacatcaca tacaaagata tactgaaagt tcagccattt ggtaatacgg
     1621 tagtaagtat tgatttgacg gggaaagaag tagctgacta cctagctaca gtggcacaga
     1681 tgaaaccgga ttccggagcc tacccacaat ttttgaatac cagctttgta gtcaaaaaag
     1741 gaaaaatcga aatgctaaaa ataaaaggta aatcggttga ccttaacaaa aaatatcgta
     1801 tgacaacatt tagctttaat gctactggtg gggatggcta tccacgtatt gataatagac
     1861 ccggatacat aaataccgga tttattgatg ctgaagtact tatcgaatat atccggaaac
     1921 attcaccgct ggatgctgct tcatatgaac caaaaggtga agtcagttgg cagtgataac
     1981 gtaattgacg atcatcacgt gttttttgct gacagtcgct gtgactttgt gaaagtagtc
     2041 tctgcgtatg tttgcgatat gggaatgcag ttgctgtatt ttgtgtttct gcttctgcca
     2101 gttgttgctg aatttaacct tgcggcttaa ctgatgttgg agcgctgcca gcttcttcgt
     2161 caatttcgct gattaaagtt tattcattat ggcatgatct gcgtaagcgg ctcgtcagaa
     2221 ccatattgat atttactaag agctcagatc aactttccag ggcaacagat cgcgtacccg
     2281 gtttgccggt cagtcctgga tatgctcaat gacgtaacgc agccattttt ctggctccac
     2341 attgttcaga cggcatgtgc cgatcagcga gtacaacacc gccgcatgtt cgccaccgct
     2401 atcggaaccc cgccggagag agtttcactc tccggctgct cacggaaccg tacgtgacac
     2461 tctcgcgtca tacggctctt gatcactcat tcgatgcacg tagtaaatac cagtgggcaa
     2521 atagcttcgg atactgtttc cttaccagcg ccagctttcg ctgagccttc cggttcgaaa
     2581 gtctgtattt agactgcatc cacttaagca aacgtgactg cattgcactc cacagtcgat
     2641 agctgaagtt tctggaccag aactttccat agtactcaat ccagcctctc actatcgcat
     2701 tgtagcgcct cgcaaaatcc agcaaactct cagctgttga gcgatgtata cgccacttct
     2761 tgattgtttc tgttattttg cacattgctg catttgatgc acccggcata cattttctgt
     2821 acaattcgcc tttgaaattc ttcagagtac gcactttgaa gtcatatccg agagtggcat
     2881 acaacatcat cggcgtacct ttcgaaggga acgccgggaa actgacgttg catccacata
     2941 tcaaaggcat agtgcaggaa cagatttgag agcagggggg agattacgcc gccctgcggc
     3001 gttcctcgac cccgtgccag cattgctcca tccggcattt gcaccggtgc cttcagcctg
     3061 cgctcaatgt acatcaccac ccagcgtgcc tgagtatgat gctgcactgt cttcagtagc
     3121 aggtcatgat cgatatcatc aacgaagcct ttaatatcga cttccaccac ccagtcaaac
     3181 ttccagcagc gtttcctcgc ctgcgcaatc gcctggtgtg cggatttacc cggtcgataa
     3241 ccatacgagt ctgcatggaa caggggttcc agtaccggct cgagtatctg cttaacgacc
     3301 atctgggcga tccggtccga cacggccgga ataccgagcg gacgtacacc accgtcagct
     3361 ttgggaatgt ccacacgcct taccgcctgc ggcatataac tgccggaagc cattctgttc
     3421 cacagtttgt agagattgtc tgtaacattc tcgtcaaacc cggccagtgt ctgcccgtca
     3481 atgcctgccg ccccacggtt ggcttttact cgtttataag cttcccatac ctgtcgttta
     3541 cttacaggaa atggctttgc cgattttaca gcagtcctcc catcggcatt gtcgcccgtg
     3601 gttgccgccg caccatcagc acaatggggc aagccctttg ctccggaccc attacggtcc
     3661 ttcaccgcta ctacggttta ctccgtccct gta
//
LOCUS       DAJTRN010000199         3582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTRN010000199 DAJTRN010000000
VERSION     DAJTRN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..504)
                     /gene="sitD"
                     /locus_tag="OO328_003978"
     CDS             complement(<1..504)
                     /gene="sitD"
                     /locus_tag="OO328_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5932332.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQV"
     gene            complement(501..1358)
                     /gene="sitC"
                     /locus_tag="OO328_003979"
     CDS             complement(501..1358)
                     /gene="sitC"
                     /locus_tag="OO328_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5932333.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1355..2182)
                     /gene="sitB"
                     /locus_tag="OO328_003980"
     CDS             complement(1355..2182)
                     /gene="sitB"
                     /locus_tag="OO328_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5932334.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2182..3096)
                     /gene="sitA"
                     /locus_tag="OO328_003981"
     CDS             complement(2182..3096)
                     /gene="sitA"
                     /locus_tag="OO328_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5932335.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT          973 a          883 c          839 g          887 t
ORIGIN      
        1 tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa tcccgccatt ttacgctaat
       61 aacaagtgta accagcccgg caataatcac cgtctggatt atgtcgccga tggttatccc
      121 gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct ggtttaattg caatatacaa
      181 gatcagacct gcggcaaaca tgccagaaaa aacaatcccc ataactgtat cttgcttgat
      241 tcggctgttg tctttcaggt atcccgtcgc caccgcgcag aatactccgg cgacgaatgc
      301 ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg atgccaggaa aaaccgcatg
      361 actcatcgcg tcacccatca gtgcccaacc tttcaggacc agaaagaccg acagcagcgc
      421 acagggtatg gaaactacca gtgaaatcag cagtgcgtga ctcataaagg tgaactgcag
      481 tggctccagt aataatgcca tcatgagcga tcctccagcg ctttatgcgc acgccgacga
      541 tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta aaagaaacag cagtgtctgc
      601 gccaccacga tgattccacc tgtcgcgccg tccagaaagt aactgaccca cgcgccgagg
      661 aaactggtta cactgccaat agttacagca attatgagca ggcggggaaa acgatcggtt
      721 aacaaccatg ctgttgcacc gggagtcacc accagacaga tcaccagaaa ggcaccaacc
      781 gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga agaagaggat ttttaatctg
      841 cccggatgta atccaatagc acgggcgtga ttctcgtcga aaaaggtcac cataagatct
      901 ttccatttga aaaaaagcac gatgatcgag agaatgccaa tgatagtgag ttgcaggata
      961 tccgccggat cgattgccag aatattgccc aggacgatgg tctgaatgtt cacggatgtc
     1021 gggttcaatg acaccataaa cagccccagg ccaaaaaagg acgagaagat caggccgatg
     1081 atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca gcatgctgcc cgctgccagt
     1141 ccgccggaaa agaaggctcc cagagaaaac ggaagcccca gcatatatgc ccccgcaaca
     1201 cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg accagccttt tagcatcaga
     1261 tagcatgaca ggaaagcgca gagaccgccg accattgccg agacccacat cgcattgagc
     1321 atatattcat agctgaaggg ttccagtagc acgttcatct tgcttccctc tgcaccgctg
     1381 acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat aatgctttct tccgagccgt
     1441 taagtgtgac atggcgcagt acgccgctaa aagccagctc caggttctcg gctgtaaaag
     1501 tggtgtctgt cggcccgctt gccaggacgg tgcctttgac catgaccgta taatcgcaaa
     1561 atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat cgttttgcct tccgcgcgta
     1621 actccctcaa caggctgatg attttcgctt cggttttcac atcaacgccg gtaaaaggtt
     1681 catccagcag gatcacatca ccctgctgtg caattgctct cgccagaaag acgcgtttct
     1741 tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa atccaccata tcgacacgtt
     1801 ccagcgcatc ggtgacaatc tgacgatccc tctttttggc tatacgcaac atacccatat
     1861 gcccatagcg gcccatcatc accacatctt ccaccagtac aggaaatgac cagtcaacct
     1921 cttctgactg agggacgtag gcaaccaggt ttttctgtag cgcctgtcgc gtgggaatac
     1981 ccagaacaga tatctttccg ctggtcagac gcacaaatcc cataatcgct ttaaacaacg
     2041 tcgattttcc ggaaccgttt accccaacca gagcggcaat agatccactt ggtactgtga
     2101 aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt gacagtaaca tcattaacga
     2161 caatgcctgc agattgcatc attatttctc ccttttcccg gctttaattc cctgtaccag
     2221 cgtactggta gtaaccttaa gaaggtcgat atacgtaggt accgggccgt tttctgtgct
     2281 cagggaatcg acatagagca caccgccgta gtgtgcgccg gtttcacgcg caacctgacg
     2341 cgctggttta tcggaaatcg tactctcgct aaagactgcc gggatatgat ttttcttaac
     2401 tatatcaaca accttacgta cctgctgcgg tgttccttgt tgatcggcat taatcggcca
     2461 cagataaagc tcttttagcc ccaaatcgcg tgcgagataa gaaaaagccc cttcactggt
     2521 gaccatccat cgctgattct cagggagttc cgtaatctgc ttacgcaggg gggcaagggt
     2581 ttgggtaatc ttggctttat aagtatcggc attacgttgg taggtttgtg catttgccgg
     2641 atcgtatttt atcaacgcat cacgaatatt atcgacgtaa atcagagcat tatctggcga
     2701 catccatgca tggggattag gtttgccctc atagggtcct tcggtgatcc ctactggcgt
     2761 cacacccgaa gagacaatta cttctggaac cccattgaga tgctggtaaa agcgttggaa
     2821 ccacaattcc agattcatac cattggcgag aatcagttgt gccccctgcg cacgtttaat
     2881 atcgccaggg gtaggctgat actcatgaat ttctgcacca ggcttggtta tggatgagac
     2941 ttctgcagca tctccagcca cgtttttggc catatccgcg atgatggtaa atgttgtaat
     3001 gaccttgaat ttttcagttg cacttgcctg aaatgcgatc gagcaggtga gggcgaggca
     3061 ccccaagagc atggttactt tttttattga gtgcataatt tagtacctat agtgagcaaa
     3121 attagaacaa acatagcaac ggctatgttt tatagcataa cctatacaaa atgaaaataa
     3181 ttatcattaa cagctttgag gcctaaaaca gggattaggt tgtttcctgc tggcgacatg
     3241 atattgagga aaaaagtaga gcagctatta ggctttgaaa tgactttatg aggaaaattg
     3301 ttgatggcaa aaatggggca aaaaagagtg ggttatcgta gcttattgtt gtcgctgata
     3361 atgtttaaca tattgaaaaa taaataaaat acttatgagt cagatagttg tgatttttgc
     3421 ccttacttgt tcaggttgta ttgttctttc tttgtaattt gttgattttc ttgcattatt
     3481 tcagttctct ggtactaaat ggggcaaatt gggggcaaac tttgcaacta cgataaccgc
     3541 gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa tc
//
LOCUS       DAJTRN010000200         3514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTRN010000200 DAJTRN010000000
VERSION     DAJTRN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            314..649
                     /gene="iraM"
                     /locus_tag="OO328_003982"
     CDS             314..649
                     /gene="iraM"
                     /locus_tag="OO328_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5932336.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(930..1038)
                     /locus_tag="OO328_003983"
                     /pseudo
     CDS             complement(930..1038)
                     /locus_tag="OO328_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1561..1637)
                     /locus_tag="OO328_003984"
     tRNA            complement(1561..1637)
                     /locus_tag="OO328_003984"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Arg,seq:tct)
     gene            complement(1739..1814)
                     /locus_tag="OO328_003985"
     tRNA            complement(1739..1814)
                     /locus_tag="OO328_003985"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:Ile,seq:cat)
     gene            complement(1959..2780)
                     /locus_tag="OO328_003986"
     CDS             complement(1959..2780)
                     /locus_tag="OO328_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5932337.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2795..3151)
                     /locus_tag="OO328_003987"
     CDS             complement(2795..3151)
                     /locus_tag="OO328_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5932338.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3164..>3514)
                     /locus_tag="OO328_003988"
     CDS             complement(3164..>3514)
                     /locus_tag="OO328_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5932339.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1015 a          936 c          668 g          895 t
ORIGIN      
        1 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
       61 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      121 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      181 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      241 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      301 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      361 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
      421 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
      481 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
      541 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
      601 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
      661 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
      721 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
      781 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
      841 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
      901 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
      961 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1021 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1081 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1141 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1201 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1261 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1321 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1381 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     1441 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     1501 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     1561 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     1621 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     1681 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     1741 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1801 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     1861 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     1921 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     1981 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2041 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2101 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2161 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2221 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2281 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2341 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     2401 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     2461 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     2521 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     2581 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     2641 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     2701 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     2761 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     2821 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     2881 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     2941 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3001 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3061 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3121 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3181 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3241 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3301 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3361 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     3421 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     3481 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTRN010000201         3465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRN010000201 DAJTRN010000000
VERSION     DAJTRN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..648
                     /locus_tag="OO328_003989"
                     /pseudo
     CDS             1..648
                     /locus_tag="OO328_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="OO328_003990"
     CDS             645..1031
                     /locus_tag="OO328_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932340.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="OO328_003991"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="OO328_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5932341.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTHDVSE"
     gene            3292..>3465
                     /locus_tag="OO328_003992"
     CDS             3292..>3465
                     /locus_tag="OO328_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR5932342.1"
                     /translation="MTSLYRIQEGCFALPETFLDRTVNIFVPSGNERATPSLNIFRDT
                     LRPDENLTTYIDRQ"
BASE COUNT          940 a          742 c          914 g          869 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccacgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccag
//
LOCUS       DAJTRN010000202         3399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRN010000202 DAJTRN010000000
VERSION     DAJTRN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..211)
                     /gene="ychE"
                     /locus_tag="OO328_003993"
     CDS             complement(<1..211)
                     /gene="ychE"
                     /locus_tag="OO328_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5932343.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQ"
     gene            688..3363
                     /gene="adhE"
                     /locus_tag="OO328_003994"
                     /gene_synonym="adhC"
     CDS             688..3363
                     /gene="adhE"
                     /locus_tag="OO328_003994"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5932344.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT          915 a          835 c          812 g          837 t
ORIGIN      
        1 gttgtagaat cgtgtcgccg agaaaaagcg agatccacaa gataatggcc acagacaggt
       61 tggctgtaag gttagttttg tttcgcgctg ccgctgtctg ataactggtc atgctgataa
      121 agacgggaat aatccctacc gggttgacca gcgcaaataa cccgatgaaa aatttgaagt
      181 aaacgggaaa atcaaaaagg gtctgaatca cggttcgctc cgaagcaaaa gccggataat
      241 gttagccata aataaggttg aaaagacgcg ctgacaatac gccttttgac agcatttttc
      301 acctcctaac tacttaaaat tgctatcatt cgttattgtt atctagttgt gcaaaacatg
      361 ctaatgtagc caccaaatca tactacaatt tattaactgt tagctataat ggcgaaaagc
      421 gatgctgaaa ggtgtcagct ttgcaaaaat ttgatttgga tcacgtaatc agtacccaga
      481 agtgagtaat cttgcttacg ccacctggaa gtgacgcatt agagataata actctaatgt
      541 ttaaactctt ttagtaaatc acagtgagtg tgagcgcgag taagcttttg attttcatcg
      601 gttaagcaaa tcatcaccgc actgactata ctctcgtatt cgagcagatg atttactaaa
      661 aaagtttaac attatcagga gagcattatg gctgttacta atgtcgctga acttaacgca
      721 ctcgtagagc gtgtaaaaaa agcccagcgt gaatatgcca gtttcactca agagcaagta
      781 gacaaaatct tccgcgccgc cgctctggct gctgcagatg ctcgaatccc actcgcgaaa
      841 atggccgttg ccgaatccgg catgggtatc gtcgaagata aagtgatcaa aaaccacttt
      901 gcttctgaat atatctacaa cgcctataaa gatgaaaaaa cctgtggtgt tctgtctgaa
      961 gacgacactt ttggtaccat cactatcgct gaaccaatcg gtattatttg cggtatcgtt
     1021 ccgaccacta acccgacttc aactgctatc ttcaaatcgc tgatcagcct gaagacccgt
     1081 aacgccatta tcttctcccc gcacccgcgt gcaaaagatg caaccaacaa agcggctgat
     1141 atcgttctgc aggctgctat cgctgccggt gctccgaaag atctgatcgg ctggatcgat
     1201 caaccttctg ttgaactgtc taacgcactg atgcaccacc cagacatcaa cctgatcctc
     1261 gcgactggtg gtccgggcat ggttaaagcc gcatacagct ccggtaaacc agctatcggt
     1321 gtaggcgcgg gtaacactcc agttgttatt gatgaaactg ctgatatcaa acgtgcagtt
     1381 gcatctgtac tgatgtccaa aaccttcgac aacggcgtaa tctgtgcttc tgaacagtct
     1441 gttgttgttg ttgactctgt ttatgacgca gtacgtgaac gtttcgcaac ccacggcggc
     1501 tatctgttgc agggtaaaga gctgaaagct gttcaggatg ttatcctgaa aaatggtgcg
     1561 ctgaacgcgg ctatcgttgg tcagccagcc tataaaattg ctgaactggc aggcttctct
     1621 gtaccagaaa acaccaagat tctgatcggt gaagtgaccg ttgttgatga aagcgaaccg
     1681 ttcgcacatg aaaaactgtc cccgactctg gcaatgtacc gcgctaaaga tttcgaagac
     1741 gcggtagaaa aagcagagaa actggttgct atgggcggta tcggtcatac ctcttgcctg
     1801 tacactgacc aggataacca accggctcgc gtttcttact tcggtcagaa aatgaaaaca
     1861 gctcgtatcc tgattaacac cccggcttct cagggtggta tcggtgacct gtataacttc
     1921 aaactcgcac cttccctgac tctgggttgt ggttcctggg gtggtaactc catctctgaa
     1981 aacgttggtc cgaaacacct gatcaacaag aaaaccgttg ctaagcgagc tgaaaacatg
     2041 ttgtggcaca aacttccgaa atctatctac ttccgccgtg gctccctgcc aatcgcgctg
     2101 gatgaagtga ttactgatgg ccacaaacgt gcgctcatcg tgactgaccg cttcctgttc
     2161 aacaatggtt atgctgatca gatcacttcc gtactgaaag cagcaggcgt tgaaactgaa
     2221 gtcttcttcg aagtagaagc ggacccgacc ctgagcatcg ttcgtaaagg tgcagaactg
     2281 gcaaactcct tcaaaccaga cgtgattatc gcgctgggtg gtggttcccc gatggacgcc
     2341 gcgaagatca tgtgggttat gtacgaacat ccggaaactc acttcgaaga gctggcgctg
     2401 cgctttatgg atatccgtaa acgtatctac aagttcccga aaatgggcgt gaaagcgaaa
     2461 atgatcgctg tcaccaccac ttctggtaca ggttctgaag tcactccgtt tgcggttgta
     2521 actgacgacg ctactggtca gaaatacccg ctggcggact atgcgctgac cccggatatg
     2581 gcgattgtcg acgccaacct ggttatggac atgccgaagt ccctgtgtgc tttcggtggt
     2641 ctggacgcag taactcacgc catggaagct tatgtttctg tactggcatc tgagttctct
     2701 gatggtcagg ctctgcaggc actgaaactg ctgaaagaat atctgccagc gtcctaccac
     2761 gaagggtcta aaaatccggt agcgcgtgaa cgtgttcaca gtgcagcaac tatcgcgggt
     2821 atcgcatttg cgaacgcctt cctgggtgta tgtcactcaa tggcgcacaa actgggttcc
     2881 cagttccata ttccgcacgg tctggcaaac gccctgctga tttgtaacgt tattcgctac
     2941 aatgcgaacg acaacccgac caagcagact gcattcagcc agtatgaccg tccgcaggct
     3001 cgccgtcgtt atgctgaaat tgccgaccac ctgggtctga gcgcaccggg cgaccgtact
     3061 gctgctaaga tcgagaaact gctggcatgg ctggaaacgc tgaaagctga actgggtatt
     3121 ccgaaatcta tccgtgaagc tggcgttcag gaagcagact tcctggcgaa cgtggataaa
     3181 ctgtctgaag atgcattcga tgaccagtgc accggcgcta acccgcgtta cccgctgatc
     3241 tccgagctga aacagattct gctggatacc tactacggtc gtgattatgt agaagatgaa
     3301 actgcagcga aaaaagaagc cgctccggct aaagctgaga aaaaagcgaa aaaatccgct
     3361 taatcagtag cgctgtctgg caatataaac ggccccttc
//
LOCUS       DAJTRN010000203         3398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRN010000203 DAJTRN010000000
VERSION     DAJTRN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(106..1815)
                     /locus_tag="OO328_003995"
     CDS             complement(106..1815)
                     /locus_tag="OO328_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5932345.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO328_003996"
     CDS             complement(2247..2966)
                     /locus_tag="OO328_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5932346.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3398)
                     /locus_tag="OO328_003997"
                     /pseudo
     CDS             complement(3091..3398)
                     /locus_tag="OO328_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1002 a          682 c          581 g         1133 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagca
//
LOCUS       DAJTRN010000204         3344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRN010000204 DAJTRN010000000
VERSION     DAJTRN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..134
                     /locus_tag="OO328_003998"
     CDS             <1..134
                     /locus_tag="OO328_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932347.1"
                     /translation="PSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            197..418
                     /locus_tag="OO328_003999"
     CDS             197..418
                     /locus_tag="OO328_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5932348.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            581..955
                     /locus_tag="OO328_004000"
     CDS             581..955
                     /locus_tag="OO328_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5932349.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1002..1376
                     /locus_tag="OO328_004001"
     CDS             1002..1376
                     /locus_tag="OO328_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5932350.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1373..1864
                     /locus_tag="OO328_004002"
     CDS             1373..1864
                     /locus_tag="OO328_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932351.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            1876..2073
                     /locus_tag="OO328_004003"
     CDS             1876..2073
                     /locus_tag="OO328_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932352.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2158..2718
                     /locus_tag="OO328_004004"
                     /pseudo
     CDS             2158..2718
                     /locus_tag="OO328_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            2790..>3344
                     /locus_tag="OO328_004005"
     CDS             2790..>3344
                     /locus_tag="OO328_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5932353.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHY"
BASE COUNT          833 a          891 c          816 g          804 t
ORIGIN      
        1 cacccagtaa ggcagaccgg cttatcaccg aacgtctggt acaggcactg ggcctggtgg
       61 atatccgggt gccggaccat ctgatagtcg gtggcaacca ggttttctcc tttgccgaac
      121 atggtctgct ttaacccgtc acaaccacat cacacctgtt ttcactttta tcttctgtct
      181 tcagaggtat cacatcatga gaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca
      241 gcatcctgca tcccgtcttt ttccgttctg taccggtaag tatcgctggc acggcagtgc
      301 tgaagcgtat actggtcgtg aagtgcagga tattcccggc gttcttgccg tgtttgcaga
      361 acgccgtaag gacagttttg gcccgtatgt ccggctgatg agtgtcaccc tgaactgatg
      421 ttccgtcacc cgggaaacga gtacgaccag atgacctcag acgccgctgt ggatgaggcc
      481 attctcatca atgcatacat cttcaccgaa accggtcagc gcgccggctg acctgtcatc
      541 gttaaaccat tcttaaccac ttttcagaga ggattttatc gtgtcagaca aactctccgg
      601 gataactcat cccgatgaca atcacgaccg cccctggtgg gggcttccct gcacagtgag
      661 gccctgtttt ggcgctcgtc tggtgcaaga gggtaaccgc ctgtattacc ttgccgaccg
      721 cgccggtatc agaggccgct tcagcgacgc agatgcatac catctggacc aggcctttcc
      781 gctgctgatg aaacaactgg aactcatgct caccagcggt gaactgaatc cccgatatca
      841 gcataccgtc acgctgtatg caaaagggct gacctgcgaa gccgacaccc tcggctcctg
      901 tggttacgtt tatctggctg tttatccgac accagcaccc gcaaccacct cataaccccg
      961 aagctctcac ctttagcact accagttaca gagaacttaa gatgaaaaca ttatccgaca
     1021 cacatgtacg ggaggtatct cgctgcccgt ctcccgtcac catctggcag acactgctca
     1081 tccgactgct ggaccagcat tatggcctca cactgaatga cacaccgttt gtcgatgaac
     1141 gtgtgattga gcagcatatt gaggcaggca tttcactgtg tgatgcagtt aattttctcg
     1201 tggaaaaata cgcgctggtg cgtaccgacc agccgggatt cagcacctgt ccccgctctc
     1261 agttaataaa cagtattgat atcctccggg ctcgccgggc gaccggcctg atgacccgcg
     1321 acaattacag aacggtaaat aacattaccc tgggtaagta tccggaggcg aaatgatgaa
     1381 actggccctg acactggaag ccgacagcgt taacgtacag gcactgaaca tagggcgcat
     1441 tgtcgttgac gtcgatggta ttgagctcgc tgaactgatt aacatggtct gcgataacgg
     1501 ctactccctt cgtgttgttg atgaatctga ccagacctca gcagaatgca cgccaccatt
     1561 tgctaccctt accggcatac gctgcagtac cgcacatatc acggaaacgg acaacgcctg
     1621 gttgtactcg ctgtcacacc agaccagtga cttcggtgaa tcagaatgga ttcattttac
     1681 aggtagcgga tatctgttac gtaccgatgc gtggtcatac ccggttctgc ggcttaaacg
     1741 cctggggctg tcaaaaacgt tccgtcgtct ggtcgtcaca ctcatccggc gttatggcgt
     1801 cagtctcatt catctggatg ccagcgctga atgcctgccg ggtttaccca ctttcgactg
     1861 gtaaccagga acaacatgaa atcattaacc acggaaacag cgctggatat tctgattgcg
     1921 tggctgcagg acaatatcga ctgcggatcg ggaattatct ttgacaacga tgaggataaa
     1981 acggattcag cagcactgtt gccctgtatc gaacaggccc gggaggatgt ccgtaccctg
     2041 cgtcatctgc agcttctgca ccagaaccgg tgaagctcac tcaccagact tcattcctct
     2101 gacgccagcc tgcacagggc tggcgttttc atttatctgc aaaaaggaat atcgattatg
     2161 tcagatatca caatctcccg tccggaagtg gtcaacgggc atacggacgt tatctgctca
     2221 accagcattc gccacattct ggctgtacga aagagtacac tgttgcaaat cgacacactt
     2281 atccgacaac tggctgaaat ctcggtgctg acggaaagca tcggaggtaa aactgccccg
     2341 gactgggcca tgaaacagga ttttcgctgc ggctgctggc tgatggagaa acccgaaaca
     2401 gcaatgaaag ccattacccg gaatcttgat cgcgaaatct ggcgtgacct gatgcaacgt
     2461 tccgggatgc tgtcattaat ggatacgcag gcccgtgata catggtaccg gtcactggag
     2521 tacgataatt ttccggaaat cagcgaagcg aacattctga gcacatttga acaactgcac
     2581 cagaataagg atgaggtgtt tgagcgaggg gtgatcaacg tcttcagagg gctgaactgg
     2641 aattacaaaa ccaattgccc ctgtaaattt ggcagtaaaa ttatcgtcaa caatctggtg
     2701 agatgggacc ggtggggata atgatatggt cacccccacc ttaacagcag tacgctgtta
     2761 aggtaaatcc ctttaacgga gagaccatca tgcagaacat cacacttatt ggtatcgatc
     2821 tcggaaagca ctccttccac attcattgtc aagacaagtc tggtaaggct cttctgcgta
     2881 aaaaatttac ccgtactaaa ctgatgcagt ttctggcgac atgtccgccc tcagttgttg
     2941 taatggaagc ctgtgcgggt gcacacttta tggctcgctg tatctctgat tttgggcatg
     3001 aaactaagct gatttctcca cagttcgttc gcccgtttgt caaaagtaat aagaatgatt
     3061 ttgttgatgc agaagccata tgcgaagcgg cttcacgacc atcaatgcgt tttgtccagc
     3121 caagaactga gacccagcag gccatgaggg cgttacaccg ggtcagagaa tcgctcatca
     3181 gagataaggt gaaaacaacc aaccagatcc acggtttctt gctggaattt ggaatcagcc
     3241 tgccgatagg tgatgctgta ataaaacggt tgtctctcgt gttggctgaa cacgaaatcc
     3301 ccgaatatct gagccgcctt ttggtgaggt tgcataccca ctac
//
LOCUS       DAJTRN010000205         3269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRN010000205 DAJTRN010000000
VERSION     DAJTRN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1447)
                     /locus_tag="OO328_004006"
     CDS             complement(<1..1447)
                     /locus_tag="OO328_004006"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5932354.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGI"
     gene            1579..2688
                     /gene="apbC"
                     /locus_tag="OO328_004007"
     CDS             1579..2688
                     /gene="apbC"
                     /locus_tag="OO328_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5932355.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            2950..3231
                     /locus_tag="OO328_004008"
     CDS             2950..3231
                     /locus_tag="OO328_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5932356.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          801 a          806 c          846 g          816 t
ORIGIN      
        1 tgatgcccat tgagcaaatc gcctgcaggt cagcatcgcg accttcctgt tttgcgacta
       61 cccacggagc ctgctcgtcg acatagcggt tagccaggtc agccagtgcc atgatttcgc
      121 gcactgcttt accaaattca cggctttccc acgcttcacc aatcacttca gcggcatcgg
      181 taaaggtttt gtacagctca gggtcagcca gctcgcttgc cagcacaccg tcaaaacgct
      241 tgttgataaa gcccgcatta cgggaggcca ggttaaccac tttgttcacg atatcggcat
      301 tcacacgctg aacgaaatct tccaggttga gatcgatatc atcaatgcgc gaagagagtt
      361 tcgcagtgta gtagtaacgc aggctgtcag cgtcaaaatg attcagccag gtgctggctt
      421 taataaaggt gccgcgagac ttggacatct tcgcgccgtt caccgtcaca tagccatgaa
      481 caaacaggtt ggtcggcttg cggaagttgc tgccttccag catggcaggc cagaacaggc
      541 tgtggaagta aacgatatct ttaccgatga agtggtacag ctcggcggtg gagtctttct
      601 tccagtattc atcgaagctt acgctgtcgc cgcgcttgtc gcacagattc ttgaaagaac
      661 ccatgtagcc aatcggtgcg tccagccaga cgtagaaata tttgcccggt gcgttcggaa
      721 tttcaaaacc aaagtatggc gcgtcgcggg agatatccca ctgttgcagg ccggattcaa
      781 accactcctg cattttgttt gccacctgct cctgcaacgc accgctgcgg gtccatgcct
      841 gcagcatttc gctgaaagag ggcagatcaa agaagaagtg ttcagaatca cgcatcactg
      901 gtgtagcacc agaaaccacc gatttcggct cgatcaattc agtcgggctg taggtcgcgc
      961 cgcagacttc gcagttatcg ccgtattgat ccggggattt acatttcggg caggtgcctt
     1021 tcacaaaacg gtccggcagg aacatgcctt tttccggatc gtacagctga gagatggtgc
     1081 ggtttttaat aaaaccgttt tctttcaggc gagagtagat aagttctgac aactggcggt
     1141 tctcttcgct gtgcgtcgag tgatagttgt catagctgat gttaaagcct gcgaaatcag
     1201 tctgatgctc ctgactcatt tcgccaatca tctgctccgg ggtgataccg agctgctgag
     1261 ctttcagcat gatcggtgta ccgtgggcat cgtcggcgca gatgaagttg acctcgtggc
     1321 cgcgcattcg ctggtaacgg acccagacat cagcctggat gtgctccagc atatggccga
     1381 ggtggattga gccgttagcg tacggcagtg cgcacgtcac cagaattttc ttcgcgactt
     1441 gagtcatagt aggcattact tcttaatagt gaaaaggggt tagatagtac caaatgggaa
     1501 aatgttaagt aagtacttct ctatattctt caggtaaact atcagctgat ggtttatcta
     1561 tacaaaaaag gagtcgggat gaacgaacaa tcccaggcca aatcaccaga agccttgcgc
     1621 gcaatggtcg ccgggacact ggccaatttt cagcacccaa ccctgaagca taacctcacc
     1681 acgctgaaag cgttgcacca tgtcgcctgg atggacgaca cgttgcatgt ggaactggtt
     1741 atgccgtttg tctggcatag cgcgttcgaa gagttaaaag agcaatgtag cgccgaactg
     1801 ctgcgcatca ccggggcgaa agctatcgac tggaaactgt cgcataacat agccactctg
     1861 aaacgcgtga agaatcagcc gggaattaac ggtgtgaaga acattatcgc ggtgagttcc
     1921 ggtaaaggcg gcgtgggtaa atcgtccacg gcggtaaacc tggcgctggc actggcggcg
     1981 gaaggggcga aagtcggcat tctggacgcc gatatctacg gcccatcaat tcccaccatg
     2041 ctgggcgcag aaaatcagcg tccaacttcg ccggacggta cccacatggc accgattatg
     2101 tctcatggcc tggcaaccaa ttcaattggt tatctggtca ctgacgacaa tgcgatggtg
     2161 tggcgtggac cgatggcggg caaggcgtta atgcagatgt tgcaggaaac cttgtggccg
     2221 gatctcgact atctggtgct tgatatgccg ccaggcaccg gtgacattca attgacgctg
     2281 gcgcaaaaca ttccagtaac tggtgcagtc gtggtcacca ccccgcagga catcgcgttg
     2341 atcgatgcga aaaaaggcat tgtgatgttc gagaaagtcg aggttccggt gctcggtatt
     2401 gtcgagaaca tgagtgtgca tatttgcagt aactgtggtc accatgaacc gattttcggt
     2461 accggtggtg cagagaaact ggctgaaaaa taccatacac agctgctggg gcagatgccg
     2521 ttacacatct ccttacgtga ggatctcgat aaaggcacgc caacggttat cagccgtccg
     2581 gagagcgaat ttaccgctat ctaccgccag ctggctgacc gcgttgcagc tcagctctac
     2641 tggcagggtg aagtcattcc tggcgagatt tccttccgcg cggtctaacg cctattaacc
     2701 attctggtta ttcttcatac accgcagaga tgttatattg atgcggtgta tttgtttttg
     2761 ccgcattaat gaacacttat ttaatactgc gaatattctg cggaccttcg tccgaaactc
     2821 tacaaagtcg agtgatcgta caggcaatgc ttcattatca tccttcgtgc tgtgtagtgg
     2881 atgtacagct atagtttgtt atgcactcat tctatccagg cataaccttt actacaggga
     2941 taacgttcaa tgaataagta ttggctgtca ggaattattt ttcttgccta tggattggct
     3001 tctcccgcag tctcttcaga gactgccaca ttaacgatta atggcaggat cagtccccca
     3061 acatgcagta tggctatggt taatagtcaa ccacaacagc attgtggtca gttaacgtat
     3121 aatgttgaca cccgtcatca ggtttcttcg cccgtcaaag gtgttacgac agaagtggtt
     3181 gttgcaggta gcgatagcaa acgtcgaatc gtgttaaatc gctacgatta atttggcaac
     3241 ctgattttaa ttgcatttag aaagacgaa
//
LOCUS       DAJTRN010000206         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTRN010000206 DAJTRN010000000
VERSION     DAJTRN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..442
                     /locus_tag="OO328_004009"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO328_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO328_004010"
     CDS             1026..1742
                     /locus_tag="OO328_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932357.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO328_004011"
     CDS             2294..2698
                     /locus_tag="OO328_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932358.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="OO328_004012"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="OO328_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAJTRN010000207         3225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTRN010000207 DAJTRN010000000
VERSION     DAJTRN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..3087
                     /locus_tag="OO328_004013"
     CDS             <1..3087
                     /locus_tag="OO328_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5932359.1"
                     /translation="TFSINFDDSVDKTAETDEEREARYAALQQEWRQYLYDSERLLKS
                     VAGDWIEKPEQYELAEHGYIVKTAQSGGASSHILSLYDHLLVCNKDVPLFNRFASREV
                     HAAESLLAPGAKFSDRLGHSGDKFPLAKAQRDALSHFLDARHGDILAVNGPPGTGKTT
                     LVLSIIATQWARAALEKSEPPVIIATSTNNQAVTNIIEAFGKDFSQGSGAMAGRWLPE
                     LKSFGAYFPSSSRKAEAAKKYQTEDFFNQVESKEYVEDALLFYLEKAKAAFPGKECSS
                     PEKVIELLHGQLAAKSEQLIRLNATWQTLSQIRAARELIANDIEQYLDNLNKLLSGQE
                     QKVTLLKSAKTEWKKYRAGESLIYSLFSWLPAVRNKRQYQIQLFLEDKLGALIAGNQW
                     SDPETIERNIDGLLNSAEREQTTYRQQIDSAHEIVLKEQQAVQEWQRLAFDLGYEGDE
                     ELSFSQADELADTQIRFPAFLLTTHYWEGRWLMDMASIDDLQDEKKKKGAKGVTARWQ
                     RRMKLTPCVVMTCYMLPGNMQISEHKGQRKFEKSYLYDFADLLIVDEAGQVLPEVAAA
                     SFALAKKALVIGDTEQIPPIWSIAPAIDVGNMLAEKILSGSTQEEITEKYTAIADLGK
                     SAASGSVMKIAQFASRYQYDPELARGMYLYEHRRCYDNIIGYCNTLCYHGKLLPKRGR
                     EESNLMPAMGYLHIDGKGELASSGSRYNLLEAETIAVWLAENQQNIEAHYGKSLHEVV
                     GIVTPFSAQVSTIKQVLGKQDISTGTNEKSLTVGTVHSLQGAERAIVIFSPVYSKHED
                     GGFIDSDNSMLNVAVSRAKDSFLVFGDMDLFEVQPASSPRGLLAKYLFESEKNALSFD
                     YKERKDLKTAGTKIYTLHGVEQHDNFLNQTFENTSKHITIISPWLTWQRLEQTGFLDS
                     MIAACSRGINVTIVTDRSYNTEHNDFEKRKEKQQNFKAALEKLNALGIATKLVNRVHS
                     KIVIGDDGLLCVGSFNWFSATREARYERYDTSMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT          909 a          668 c          837 g          811 t
ORIGIN      
        1 acgttctcta tcaactttga tgacagcgtt gataagactg ccgaaacgga tgaagaacgg
       61 gaagcacgat atgccgcctt gcagcaggag tggcgtcaat atctgtatga ctcagagagg
      121 ctactgaaga gcgttgccgg cgactggatt gaaaaacctg agcaatatga actcgctgag
      181 cacggttata ttgttaaaac ggctcaatct ggcggtgcca gttcccatat cctttctctt
      241 tatgatcacc tgcttgtttg caataaggat gtgccgctct tcaatcgctt cgcctcgcga
      301 gaggttcatg ctgcagagtc tttgctggcc ccaggagcaa aattcagcga caggcttgga
      361 cactccggag ataagtttcc gctggcaaag gctcagcgcg atgccttaag ccattttctg
      421 gatgcaagac atggcgatat ccttgctgtt aatggccctc cgggaaccgg aaaaaccacg
      481 ctggtgcttt ctatcatcgc cacgcagtgg gccagagcgg ctctcgaaaa atctgagcct
      541 ccggttatta tcgcgacttc aacgaataac caggctgtaa cgaacattat tgaggcattc
      601 gggaaagact tttcgcaagg ttcaggtgcg atggccgggc gatggttgcc agagctgaaa
      661 agcttcggtg cttattttcc ctcaagcagt cgtaaagctg aggcagccaa aaaatatcaa
      721 actgaagatt tcttcaacca ggttgagtca aaagagtatg tagaggatgc actgctgttt
      781 tatctggaaa aggctaaggc agcctttcct ggaaaagagt gttcatcccc tgaaaaggtc
      841 attgaactct tgcatggtca gttggcagca aaatctgagc aactgataag actgaacgca
      901 acatggcaaa cgttaagcca gattcgggct gcgcgtgagc ttattgctaa tgatattgag
      961 caatatctcg ataatttaaa taaattactt tccggacaag aacaaaaagt cactctactg
     1021 aagagtgcta aaacggaatg gaaaaaatat cgcgccggtg aatcactgat ctattcatta
     1081 ttttcctggc tcccggcggt tcgcaataag cgacagtacc aaatacagct gtttctcgaa
     1141 gataaattag gcgcgctgat tgcaggaaat cagtggtctg atcctgaaac tatcgaacgt
     1201 aatattgatg ggctgctcaa ttccgctgag cgcgagcaaa caacataccg gcagcagatt
     1261 gactccgccc atgaaatcgt tcttaaagaa cagcaggcgg ttcaggagtg gcagaggctg
     1321 gcatttgatt tagggtatga gggcgacgag gaactgagct tctcacaggc cgatgaactg
     1381 gctgatacgc agattcgctt ccctgcattt ttactgacga ctcactactg ggaaggtcgt
     1441 tggctgatgg atatggccag cattgatgat ctgcaggatg agaagaagaa aaaaggtgct
     1501 aaaggggtaa ccgcccgttg gcaacgtcga atgaaactca cgccatgtgt ggtgatgaca
     1561 tgctatatgc tgcccggtaa tatgcagata agtgagcaca aaggacaacg taaattcgag
     1621 aaaagttatt tgtatgattt tgccgattta ctcattgtcg atgaagccgg gcaggtgctt
     1681 cctgaagtgg ctgctgcctc gtttgcatta gctaagaagg cattagtgat tggcgatacg
     1741 gagcagatcc cgccaatatg gagtattgct cctgcgattg atgtcggtaa catgctggcg
     1801 gaaaaaattc tgtctggcag tacgcaagaa gagattaccg agaaatatac ggcaatcgca
     1861 gaccttggta aaagtgccgc atctggcagc gttatgaaaa tagcgcagtt tgcttcgcgc
     1921 tatcaatatg atcccgaact ggctcgtggt atgtacctat atgaacaccg ccggtgctac
     1981 gacaatatta ttggatactg taatacgctc tgctatcacg gtaagttgtt gcctaaaaga
     2041 gggcgtgaag agagcaattt aatgcccgca atggggtatc tccatattga tggtaaagga
     2101 gagctggcaa gtagtggaag tcgatataat ttgcttgagg ctgaaacgat agcggtctgg
     2161 ttggcagaga accagcaaaa tattgaagcg cattacggta aatcgcttca tgaagttgtc
     2221 ggtattgtga cgccttttag cgctcaggta tccactatca aacaggtgct gggcaaacaa
     2281 gatatcagta caggcacgaa tgaaaagtcg ctcacagtgg gcaccgtgca ctctcttcag
     2341 ggagcggaaa gagcgattgt gatattctcg ccagtctatt caaaacatga agacggcggg
     2401 tttattgata gcgataacag catgctgaat gttgcagtct cccgtgcgaa ggacagtttt
     2461 ctggtcttcg gcgatatgga cctgtttgag gtccagccag cctcatcgcc acggggatta
     2521 ctggcaaaat acctctttga gtcagagaag aatgcgctct cttttgatta taaagagcgt
     2581 aaggatttaa aaaccgccgg gaccaaaatc tacacacttc atggtgtgga gcaacatgat
     2641 aatttcctga atcagacatt tgaaaatacc agtaaacaca tcacgataat ttctccatgg
     2701 ctgacctggc aaaggctgga gcaaaccggt tttcttgatt ccatgattgc ggcgtgttca
     2761 cgtggaatta acgtcacgat agtcactgac agaagctaca acactgaaca taatgatttt
     2821 gagaagcgaa aagagaagca gcagaacttt aaagcggcgc tggagaaact gaatgcgctg
     2881 ggtattgcta caaagctggt aaaccgtgtt catagcaaaa ttgttattgg tgatgatggt
     2941 ttgctgtgtg tgggatcgtt caactggttt agtgcgacac gggaagcgcg atatgaacga
     3001 tacgatacat caatggttta ttgcggtgat aacctgaagg gtgagattga ggctatttat
     3061 aatagtcttg agaggcgtca ggtttagagc aggaatggtg agtagccagc ttaccgatcg
     3121 ttttcgagcg taagatggct gaatggaatg gctattattg cacagtcctt aattataaca
     3181 ttcataccga catgattatc ttctgtccgg aagaatcaga ggctc
//
LOCUS       DAJTRN010000208         3175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRN010000208 DAJTRN010000000
VERSION     DAJTRN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            9..647
                     /locus_tag="OO328_004014"
     CDS             9..647
                     /locus_tag="OO328_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5932360.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            650..1813
                     /locus_tag="OO328_004015"
     CDS             650..1813
                     /locus_tag="OO328_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5932361.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1897..>3175
                     /locus_tag="OO328_004016"
     CDS             1897..>3175
                     /locus_tag="OO328_004016"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5932362.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          703 a          755 c          927 g          790 t
ORIGIN      
        1 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
       61 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      121 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      181 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      241 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      301 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      361 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      421 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      481 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      541 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      601 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      661 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      721 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
      781 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
      841 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
      901 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
      961 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1021 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1081 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1141 tgagctttac agtcgggaac tgttttattt ttgctgctgt caggacaccg atgaagacag
     1201 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1261 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1321 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1381 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1441 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1501 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1561 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1621 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1681 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1741 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     1801 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     1861 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     1921 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     1981 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2041 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2101 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2161 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2221 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2281 gctgggtcgt tatgtccagt aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2341 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2401 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2461 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2521 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2581 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2641 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2701 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     2761 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     2821 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     2881 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     2941 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3001 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3061 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3121 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggtt
//
LOCUS       DAJTRN010000209         3150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRN010000209 DAJTRN010000000
VERSION     DAJTRN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            801..1910
                     /gene="fepE"
                     /locus_tag="OO328_004017"
     CDS             801..1910
                     /gene="fepE"
                     /locus_tag="OO328_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5932363.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2935..3129)
                     /locus_tag="OO328_004018"
                     /pseudo
     CDS             complement(2935..3129)
                     /locus_tag="OO328_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          798 a          652 c          759 g          941 t
ORIGIN      
        1 ggtggtaatt gtaagacgtt gcgggcgacg gtattacaat tgcacatcct gtcctgtttc
       61 tttaggacgt atcatggcac agcatatcaa ctgataaata atccctgtgc agtgcagccg
      121 ctgttgcata aaatttcgct ctgcgtcgag tgtttcttaa tcaataattc cgctgatact
      181 ccggaaaaac gcgagtctgt tctcatgcct gaaatgccca caccccacgc aaaaacaagt
      241 ttttgctgat ttttctttat aaatagagag ttatgacaaa ttagttcttc ttgctctctt
      301 tgtgatattt aaaaaagcgg tgtcggcgcg gtgttgtagc tgcgccaaca ccgcttttta
      361 ggggtggtac tgactatttt cataaaaaaa acattatctt atattagggg tgctgctagc
      421 ggcgcggtgt gtttttttac aggacgcccc tgggggcgct gctaggggtg tctgttcaga
      481 tatgtagcaa tgagtcaggg attcacgaac tgaaaatgtc cgaaaatgtg tctcatgggc
      541 actgtatgcg cccaaaccga tcgtcattca ctttcatgca tagctatgca gtgagctgaa
      601 agcgatcctg acgcattttt ccggtttacc ccggggaaaa catctctttt tgcggtgttt
      661 gcggcagaat cgcgttcagc gcgttttagc ggtgcgcgca ataaaatgtt atgttaaatg
      721 tctgctgaat ggtttttagg ttttttccta acatatgata gaataaaaaa accatatgtt
      781 aatatattgt gtttttttca atgaaaatct caaaggtctc tgacgaacat ttttctaatt
      841 acaacatgtt gccgaatggt aatgaagaaa ttgacctttt tggcttgatt agcgttttat
      901 gggcagcaaa aataaaaatt gttggggtga ttctattttt tgcttgtatt ggctttcttg
      961 tttcttttgt tttgccacaa aaatggacta gttctgctgt ggttactcct gctgagtctg
     1021 ttcaatggag tgaattaaat aaggagcttt ccaagcttca tgttctggga gtggattttg
     1081 atatcaatcg tgatagcgca tttgctctgt ttattaagaa atttcagtcc gttaattcgt
     1141 tgaatgagta tatgagatca tctccttatg ttatggagat tattaaaaag aataacatta
     1201 gcgctctcga actgcatcgt gcaattgttg agttgagtaa aaatatgaag tcgattgatg
     1261 ataatgccag taagaaaaac gacaagtcat cattgtatgt atcatggact ctgagtttta
     1321 ctgctccaac aagtaaagaa gctcacgatg tgttgtctgg gtatattaat tatgtttctt
     1381 cccttgttgt aagggatttg atggaagata taagaaataa actagaagtt aaaactaatg
     1441 ttgaaaaaga aattcttgca ctggatgaga taaaaattag aaaccagctg aatgcagata
     1501 ttcgacgcct caattattca ctggaggttg ctaatgcggc tggaataaaa aaacctgtat
     1561 acagcaatgg tcagattatg aaggatgacc cagattttcc tgtggctctc ggttctgatg
     1621 gtatagcaac taaattgaac atcaaaaaat caatcaagga tgtttcggaa ttgagtgggg
     1681 agttgcgaaa tcgtcaatat gttgtgaatc aattggttgt ggcgaaagtg ggggatgttg
     1741 attttatgcc atttcagtat caattgagcc caacgttacc ggtcagaaag gatgggccaa
     1801 gtaatgccat aattgtaatt ctttcttctg tcattggagg gatgcttgca tgtggctata
     1861 tacttttacg tcatgccatg gaggccagaa agatgagtgt gattgagtaa aagttgtgct
     1921 gatgcatcat tttataagca acttttataa aaagttcaaa ggatcttctt gagatcattc
     1981 ccttacagcc gtagtcttct actctagaaa acgaaaaaac cacctgggga ggtggttttt
     2041 cgaaggttca gtaagttggg gaacttctga accgtggtaa cagtcttgtg cgagacatgt
     2101 caccaaatct gtccttctag tgtagcctca gtaaggctgc cacttcaaga actctcaata
     2161 catctctcgc acatcctgtt taccagtggc cgctgccaat ggcgttaagt cgtgtcttac
     2221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2281 ttcgtgcaca cagcccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     2341 aatgagacga acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     2401 gagagcgcac gagggagcca ccagggggaa acgcctggta tctttaagtc ctgtcgggtt
     2461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2521 gaaaaacgcc accggcgcgg ccttttttcg gggcctcttt tttgctcaca tgttttttct
     2581 ggtgttatcc cctgattctg tggataaccg tatatccgtc tttgcgtgag cagacaccgc
     2641 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccg
     2701 gaagctgcat tttctcctta cgtatctgtg cagcatttca cacctcatat gtcgctgttc
     2761 tctcaccatc tgctctgccg cctcataatt aagccagtat acactccgtt atcgctacgt
     2821 gactgggtca tggctgcgcc ccgacacccg ctaaaacccc ctgacgcgcc ctgacgggct
     2881 tgtctgctga cgaaaacgcc cacgggaaag ccgtgaccgc ctccgtcagc ttcgttatgt
     2941 tcctgtttca ctttcggcct caaagcgaat ctggatgctg ttctggagtt cttctgcgag
     3001 ttcgtgcagt cgtttgcaca tggccgcctg ttcgtcggca tccagagcat ccagtttttc
     3061 aagcagtgtc aggctctggc ttttgataca tcgcgccata ttcagcgcag tttgttgtta
     3121 tttgttcacc ttccgttgtt ctccgtcagt
//
LOCUS       DAJTRN010000210         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRN010000210 DAJTRN010000000
VERSION     DAJTRN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO328_004019"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO328_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5932364.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO328_004020"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO328_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO328_004021"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO328_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5932365.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO328_004022"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO328_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5932366.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OO328_004023"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OO328_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTRN010000211         3110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRN010000211 DAJTRN010000000
VERSION     DAJTRN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            453..1280
                     /locus_tag="OO328_004024"
     CDS             453..1280
                     /locus_tag="OO328_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5932367.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1324..2073
                     /locus_tag="OO328_004025"
     CDS             1324..2073
                     /locus_tag="OO328_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5932368.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2070..2639
                     /locus_tag="OO328_004026"
     CDS             2070..2639
                     /locus_tag="OO328_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932369.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2763..3005
                     /locus_tag="OO328_004027"
     CDS             2763..3005
                     /locus_tag="OO328_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5932370.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
BASE COUNT          872 a          754 c          792 g          692 t
ORIGIN      
        1 cgccatcctg acaagcgttt tatcttcaac gggaatgaga tatggaaacg tggagaatca
       61 accacagagt atacccgcgc acaccagcga acaatcagac gactgcgcag actcatcgcc
      121 aggaaacagt atacagaaaa atggttcaga aaatatgaca catggagcgc cggatattac
      181 gaactgatgg caacaaaacc attctgacgt aaacgaaatt aaccatgacg caattaaata
      241 aggcaagccg aatacatcag gaggaccatg aacatttatt tcagaatagt tatatcactg
      301 gcaattatcg catgtattta cggattaatc gttccggccc tcatatcaat gaaggatacg
      361 gtagcagtga tttctggctt tgctctggcg tgtctgaccc cgccctgcat ttatgccatt
      421 tataagggtc tttctttttc taaggataaa agatgaaaaa agttattcct gctttagccc
      481 ttgttctgct gaccaccgga cttgtcggtt gcgatcgcgt tgagcctggt aatgttggca
      541 tcaaggtaaa caaactgggc gacgataaag gcgtcggaga agtggtcggt gttgggcgct
      601 actggacggg atggaacact gaggtttaca tcttcccgac cttcaaacaa atgaagacct
      661 acgatgagcc attcagcttc cagatgagtg acggtacaac catcggctat cacatcggcg
      721 tggcctacaa agttgatcca tccaaagtta ccacagtctt tcagacctac cgcaaaggcg
      781 tggacgacat taccgacacc gacctgcgcc agaagatagc cgacgcactc aaccgactgg
      841 ccagcaaaat gaccaccgac aaatttatcg acggcggcaa gtctgagctg ctggatgcag
      901 ctcttaaaga cattcaggaa gaaatgacgc ccatcggtat tcaggtaatg agcctctcat
      961 atgtgggtaa gccggagtac ccgcctactg ttatcgacag cattaatgcc aaagtcacgg
     1021 cgaaccagaa aaccctgcaa tgcgagcagg aagtaaaaca gcgcgaagcg gaagccaaca
     1081 tgttgcgcgc ggaagctgcc ggacaggcag atgcgattcg cacaaaagcc caggccgaag
     1141 ccgatgctat tcgtttacgc ggtgaagctc tgcgccagaa cccaaacgtt atggagctgg
     1201 aagccatcaa caagtggaac ggtacactgc cgcaatacat gaccagcggt gccaacacac
     1261 catttattca ggttaagtaa tacatatgcc cggtattaca cgccgggctg tctggagata
     1321 aaaatgaata ctgtaaccat caacaacaaa cagtttccgg taatcgaata tcgcggtcag
     1381 cgtgttgtga cgctggcgat gattgatgaa gtccaccagc gaccggaagg aaccgcgcgt
     1441 gcagcattca accgaaaccg tgagcatttc atcagcggtg tggattatgc cgaattaggt
     1501 gcggacgtaa tacgtacgga cctcccggaa ggaacattct ctaaatttgc accatcgggg
     1561 attgtacttt ttgaatcagg ttacctgatg ttgacgaagc cattcaatga cgcccttgcc
     1621 tggcaggttc agcgcgaact ggttaacagc tacttccgca ctcgcacacc gctaacggaa
     1681 atcgagatga tcgctgcgat agccgccgac gctgttcgtc aacagaagcg cctgaatcat
     1741 gtggaagaac agctcgaaac agtcacagaa gcggtggaaa ccatcaaacg cggaaatatg
     1801 cgcgccggat atgtcggtta ccgccaggtg gtagccaaaa gtggaatgag tgacaccaag
     1861 tgtcggaatc tggtgaacgc atatcaaatt cctacagaca cccacgaatt catgactccg
     1921 gacggtctgt tgtctcgcag ggctatcgtc gagcttgagc catttatggc ggcgttccac
     1981 caaatgatgt cagaggctga cccacgcggc acacgctggt atcacccgaa gatggggctt
     2041 ttccaggtga tcggatggga ggataaagca tgatcatcca gtcaaaactt attcgcgccg
     2101 ctctggtgtg cgctgctaaa aacgacgttc gttattacct gaacggtctt cacatcacgc
     2161 cgaaacatat tgaggcaacc aatggttccg tggcactgcg catggctcac ggcatccgga
     2221 cgaagaaaaa catcattgtc cagttcgaag ggggcgtccc ggccaaagcc gaaacgacag
     2281 agctgatttt tagtaaagag ccgatcgctg ttcatcgcga ccagtttcag cgacgactgt
     2341 ccattaccgg cattaaactg gtggacggtt gttttccgga tttggatcgc atcattccga
     2401 aaaagtttga ccgctgtact cacccggtgc tccaggcggg ttacctgagt tatccagaga
     2461 agatgtttgg tcgtgagcgt aaatttattc ccgtccagtt acgtccctcc ggtgacgggc
     2521 aagcggtcag aattcagttt gattccatca tcaactcaat gtatggcaat cctgaatttg
     2581 ttgtgatgcc ttgtcgtgat catggcgatt tcaacgtggc tcaggagcat ccggaatgaa
     2641 aatcgaatac caggacgccg caggaggtga atcaatgagc tggcctgatg caatcgtaac
     2701 tctggggatg gtattcgcag tagcgtttgt tgtgtactcg atttgtcgat ggggataacc
     2761 acatgttcgc tttgattcaa cgcgggcaga tatacacaga cagagccgga tacccagtag
     2821 aaattactcg cagtactgag cactcagtgt tctttcggag gatggacgga cgtaccgggc
     2881 gagtacgcat cggtgaattc agtagcctgt tcgagcatat tgaccacctg gaatatcaca
     2941 agattctggc tgaaacagaa caagaaaagc acctgaagaa attacgcgca atgcaaagga
     3001 agtaaaaaat gaataaagcg tttgagctat gggtccacca gcgttacggc aaccactacg
     3061 acctgacgcg agatgttgac ggtttctact gtcgtgaaat tgtgaaacga
//
LOCUS       DAJTRN010000212         3101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRN010000212 DAJTRN010000000
VERSION     DAJTRN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            2..2905
                     /locus_tag="OO328_004028"
     rRNA            2..2905
                     /locus_tag="OO328_004028"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3001..>3101
                     /gene="rrf"
                     /locus_tag="OO328_004029"
     rRNA            3001..>3101
                     /gene="rrf"
                     /locus_tag="OO328_004029"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          817 a          684 c          970 g          630 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     3061 cgtagcgccg atggtagtgt ggggtctccc catgcgagag t
//
LOCUS       DAJTRN010000213         3088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTRN010000213 DAJTRN010000000
VERSION     DAJTRN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..2181
                     /locus_tag="OO328_004030"
     CDS             <1..2181
                     /locus_tag="OO328_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5932371.1"
                     /translation="QLRYQGEKVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTM
                     PLVPDGLPVSGHATATLNLPQEPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQIT
                     RQQLTVSDGRWSWLYAGFPLSGRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAV
                     LNFGPGKLSMDNSQLPLQLTGEAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSK
                     GRVIDSLDIDEIRWPLAGVKVTQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPD
                     AGRWQWRYWGKGSFTPMNATWDVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRL
                     ILDKPVVWVRDAQHPSFSGALSLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGD
                     LHAGEIGPVRVNGRWDGIRLRGNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFS
                     AAPEQGFRAGGHGVLKGGSAWMPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEV
                     INLVTAKNITADLQGRYPWTEEEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNN
                     LSSSELVSAVNPKQFAMSGAFSGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADA
                     VVKDNMTAGSAINWLRYMEISRSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNY
                     HHEENIFTLWRSLRFGDNLQAWLEQNARLPGNDCPQGKECEEKQ"
     gene            2178..2363
                     /locus_tag="OO328_004031"
     CDS             2178..2363
                     /locus_tag="OO328_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5932372.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2368..2697
                     /locus_tag="OO328_004032"
     CDS             2368..2697
                     /locus_tag="OO328_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5932373.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(2869..>3088)
                     /locus_tag="OO328_004033"
     CDS             complement(2869..>3088)
                     /locus_tag="OO328_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932374.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
BASE COUNT          813 a          681 c          849 g          745 t
ORIGIN      
        1 caactgcgtt atcagggcga aaaagttaaa tttcaaggcc agctgaaagg gcaacaactt
       61 acagtcagcg aactggatgt cgtcgcgttt gaaaatcagc cgccggttaa actggtgggg
      121 gaatttacta tgccgctcgt gccggatgga cttcctgtaa gtgggcatgc tactgcgacg
      181 ttaaacttgc cgcaggaacc gtcactggtg gatgccgagc tggactggca ggaaaatagc
      241 gggcaattga tcgtgctggc acgggataac ggcgatccgt tgctcgattt gccgtggcaa
      301 attactcgtc aacaattgac cgtaagcgat ggtcgctgga gctggctgta tgctggtttt
      361 cctttgagtg gccgactggg tgtcaaagtc gacaactggc aggcagggct tgagaacgct
      421 ctggtcagcg gacgactgaa tgtgctgacc caggggcaag cgggtaaggg caacgcggtg
      481 cttaattttg gcccaggaaa attaagcatg gataacagtc aactgcctct gcagctgacc
      541 ggtgaagcga aacaggcgga cctcatttta tatgcccgtt tacctgcgca gctaagtgga
      601 agtctgtctg acccaacgct gacctttgag ccaggcgcgt tacttcgttc aaagggaaga
      661 gtcatcgatt cgctggacat cgatgaaatc cgctggcctt tagcgggtgt aaaagtcacc
      721 caacgtggtg ttgacggacg tttgcaggcc attttgcagg cgcatgaaaa tgaactgggc
      781 gatttcgtgc tgcatatgga tgggctggcg aatgattttc tccctgacgc tggccgctgg
      841 cagtggcgct actggggaaa agggagtttt acaccgatga atgccacctg ggatgtcgca
      901 ggaaaaggtg agtggcatga cagcacgatt acgctgaccg atctctccac cggtttcgac
      961 cagttacaat acggtacgat gacggtagaa aagccgcgat taattctcga caagcccgtc
     1021 gtctgggtac gtgacgcaca gcatccctcc ttcagcggcg cgctgtcact ggacgccggg
     1081 caaacgctgt tcactggcgg cagtgtgtta ccgccatcaa ccttaaaatt tagcgtcgat
     1141 gggcgcaatc cgacctattt cctctttaaa ggcgatttac atgctggtga gattggcccg
     1201 gttcgggtaa atggtcgctg ggacggtatt cgtctgcgcg gtaacgcctg gtggccaaaa
     1261 caatcactga ctgtattcca gccgctggtg ccacccgact ggaagatgaa cttacgcgat
     1321 ggtgaactgt atgctcaggt tgcattttct gctgcgcctg aacaaggatt ccgcgcggga
     1381 gggcacggcg tgttgaaagg cggtagtgcc tggatgccag ataatcaggt taacggtgtc
     1441 gattttgtcc tgcctttccg ttttgccgat ggagcctggc atctggggac tcgcggcccc
     1501 gttacgttgc gaattgccga agtgattaat ctggtgacag cgaaaaatat tacggctgat
     1561 ttgcaagggc gttatccgtg gactgaagaa gaacccttgc tgttgactga tgttagcgtc
     1621 gatgtgttag gcggtaacgt gctgatgaaa caattacgta tgccgcaaca tgacccggcg
     1681 ctgttgcggc tgaataatct ctcatccagc gaactggtta gcgccgtcaa tccgaaacaa
     1741 ttcgccatgt ccggggcatt tagtggtgca ttgccgttat ggctaaacaa cgaaaaatgg
     1801 atagtgaaag atggttggct ggcaaatagc gggctaatga ccttgcggct ggataaagat
     1861 accgcagatg cggtggtgaa agacaatatg actgcgggtt cagcaattaa ctggttgcgc
     1921 tatatggaaa ttagccggtc atcgacaaaa attaacttag ataatctcgg tttattaacc
     1981 atgcaggcca acattacagg taccagtcgc gttgatggta aaagcggtac ggtaaacctt
     2041 aattaccatc atgaagagaa tatttttacg ctgtggcgca gtttacgctt tggcgataat
     2101 ctccaggcat ggttggagca gaacgcacgt ctgccgggaa atgactgtcc gcaaggaaaa
     2161 gagtgtgagg aaaaacaatg aaaattttac tggctgcgtt gacgtcatct tttatgctgg
     2221 ttggctgtac gcctcgcatt gaagtcgctg cacctaagga accgatcact atcaatatga
     2281 acgttaaaat tgagcatgag atcatcatca aggcagacaa agatgtcgaa gagctgcttg
     2341 aaactcgtag cgatcttttc tgaggcgatg atgaagaaaa cattacttct ttgtgcgttt
     2401 cttgttgggc tggtaagcag caatgtaatg gcattgactc tggatgaagc cagaactcag
     2461 gggcgggtag gtgaaacatt ttatggttat ctggttgcgc tgaaaacgga tgctgaaaca
     2521 gagaaattag taaccgaaat taatgccgaa cgtaaagcga gttaccaaca acgggcaaag
     2581 caaaataatg tgtctgtaga tgatatcgcg aaactcgccg ggcaaaagct cgtagctcgg
     2641 gccaaaccgg gggaatatgt acaagggata aacggtaaat gggtgcgaaa gttttaatag
     2701 caaaaccgca atatcgggat aacgctacag gagaaagcga ttgatctatt ttcctgaaac
     2761 aaggtgaata ttcaaaaact cctgtcaaat tgcctttcgc cctgaaaaat gcatagtcaa
     2821 atatctgttt taactaattg gcgttgcagt acatgcaacg ccaattagtt agcggaattt
     2881 acgtcgatac tcgcctggcg tcatcccaaa gcgttgctta aataccgttg aaaaatgact
     2941 ctggtcagaa aatccccaat gaaagccgat gcctgccagt ttttcatcat ctgcggcatg
     3001 gcgaatcgca tctgcacaaa aatcgagacg acggttacga atatattgcg cgactaccaa
     3061 acctttatcg gcaaacattc ggtacaaa
//
LOCUS       DAJTRN010000214         3016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTRN010000214 DAJTRN010000000
VERSION     DAJTRN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..498
                     /locus_tag="OO328_004034"
     CDS             <1..498
                     /locus_tag="OO328_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5932375.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="OO328_004035"
     CDS             578..1273
                     /locus_tag="OO328_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5932376.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3016
                     /locus_tag="OO328_004036"
     CDS             1302..>3016
                     /locus_tag="OO328_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5932377.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIP"
BASE COUNT          875 a          704 c          675 g          762 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgtt
//
LOCUS       DAJTRN010000215         2941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTRN010000215 DAJTRN010000000
VERSION     DAJTRN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO328_004037"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO328_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5932378.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO328_004038"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO328_004038"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5932379.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2941
                     /locus_tag="OO328_004039"
     CDS             2603..>2941
                     /locus_tag="OO328_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5932380.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKI"
BASE COUNT          653 a          700 c          776 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 t
//
LOCUS       DAJTRN010000216         2898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTRN010000216 DAJTRN010000000
VERSION     DAJTRN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..312)
                     /locus_tag="OO328_004040"
     CDS             complement(<1..312)
                     /locus_tag="OO328_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5932381.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="yehT"
                     /locus_tag="OO328_004041"
     CDS             complement(359..1078)
                     /gene="yehT"
                     /locus_tag="OO328_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5932382.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="OO328_004042"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="OO328_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5932383.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctatag
//
LOCUS       DAJTRN010000217         2703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRN010000217 DAJTRN010000000
VERSION     DAJTRN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..849
                     /locus_tag="OO328_004043"
     CDS             <1..849
                     /locus_tag="OO328_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5932384.1"
                     /translation="FIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGP
                     NMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVE
                     MTETANILHNATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFE
                     LTQLPEKMEGVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRA
                     RQKLRELESISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIY
                     RLKSLV"
     gene            955..1607
                     /gene="pphB"
                     /locus_tag="OO328_004044"
                     /pseudo
     CDS             955..1607
                     /gene="pphB"
                     /locus_tag="OO328_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(1658..2425)
                     /locus_tag="OO328_004045"
     CDS             complement(1658..2425)
                     /locus_tag="OO328_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5932385.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
BASE COUNT          703 a          677 c          634 g          689 t
ORIGIN      
        1 ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta
       61 ctgaatgagc catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatt
      121 attaccggcc cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg
      181 ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat
      241 cgcatcttta cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg
      301 gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta
      361 atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc
      421 gcagaaaatc tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag
      481 ctgacccagc taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag
      541 cacggcgaca ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac
      601 ggtctggcgg ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa
      661 aaactgcgtg agctggaaag catttcgccg aacgccgccg cgacgcaagt ggatggtacg
      721 cagatgtctt tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc actggaaaat
      781 ctcgacccgg attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt
      841 ctggtgtaat aacaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg
      901 aatcaaataa aaataccctg tataatagga aagcttattt tacagggtaa aaccatgcca
      961 tctacacgct accaaaaaat caatgcccat cactatcgcc atatatgggt cgttggtgat
     1021 attcatggtg aatatcagtt attacaatcc cgcttacatc aactctcttt tttccccgaa
     1081 accgacttac ttatttctgt cggcgataat attgatcgtg ggccggagag tcttgacgtc
     1141 ctgcgcctgc taaaccagcc ctggtttatc tccgttaaag gcaaccacga agcaatggcg
     1201 ctggatgcgt tcgcgaccgg cgatggcaat atgtggcttg ccagcggtgg tgactggttt
     1261 ttcgatttaa atgattgcaa caagaggcaa tagttctgtt gctgaaattc catcaccttc
     1321 cacatattat tgaaatcact aacgacataa taaaatatgt catcgcacat gcagattatc
     1381 cgggtgatga atatctcttt ggcaaagaaa tagcggagag cgaattactc tggcctgttt
     1441 atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat aaacggcgct gattatttta
     1501 tatttggaca tatgatgttt gataacattc agacgttcgc taaccagatt tatattgata
     1561 ccggatcgcc gaaaagtggg cggctgtcat tttataaaat aaggtagtct catgcttctt
     1621 ctgtgaagca tgagtaacca gatgttattt caggccatta ttcatttttc gctaccagca
     1681 aagagagatc ctgcttcgcc agcgcgcgac tggcactctc cggcaaaccg tcgtctgtaa
     1741 taatctgatc aaactcgctt aatggtaacg ccagccatgt cgccacctga ccatatttcg
     1801 tcgcatcaca gaccaaaact cgctggcggc tggcactggc aatcgcccgt ttcaccgtga
     1861 ctttatcttc cgctggcgta gaaatccccc gcacactcca tgacgatgca gaaataaaag
     1921 cctgatcaat catcaggctg cacagcatgg tcgcagcggc ttccccgaca caggaacggt
     1981 tttcccgaca cactgcaccg ccagtgtgaa taattgtgca attactgttg tcgagcaagt
     2041 agttcgcaat aacgaaatcg tttgtgacca cagtcagtga ctccatgtga atcagatgct
     2101 gtgctatcgc taacgtggtc gttcccgcat ccagatagat acaacttccc ggcagaacaa
     2161 gacttgccgc cagcttgcca atagccgctt tttgcgtcat tgccagcgca gtttttacct
     2221 gatgagaagg ttcatgcgcc acgcgtcccg gagactggac gcctccggac accagcacaa
     2281 cggctccctg ctgctccagt ttttgtaaat cccgacgaat ggtcatatgt gacacattaa
     2341 ttctgtccgt tagttcagca atactgacaa tgcctttttc agctaccatc tcaaggatga
     2401 tttggcgacg ctctacgggt atcaactttt gctccttcct ttgtcctgct gacattctac
     2461 gctatttgcc tgcgaaacgt gcgcggcgca actaacgctt agttcacata aaataacaca
     2521 aaatgttaat ttatgtgaat catctcacca tatcgttatc tcccagcgct tatgttcaca
     2581 atatcaaaca aaatatcact taaattaaca aggagagcag atgaaaacgg gatctgagtt
     2641 tcatgtcggt atcgttggct tagggtcaat gggaatggga gcagcactgt catgtgtccg
     2701 cgc
//
LOCUS       DAJTRN010000218         2629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTRN010000218 DAJTRN010000000
VERSION     DAJTRN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..127
                     /locus_tag="OO328_004046"
     CDS             <1..127
                     /locus_tag="OO328_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5932386.1"
                     /translation="QISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            166..969
                     /gene="agaC"
                     /locus_tag="OO328_004047"
     CDS             166..969
                     /gene="agaC"
                     /locus_tag="OO328_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5932387.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            959..1750
                     /gene="agaD"
                     /locus_tag="OO328_004048"
     CDS             959..1750
                     /gene="agaD"
                     /locus_tag="OO328_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5932388.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1751..2506
                     /locus_tag="OO328_004049"
     CDS             1751..2506
                     /locus_tag="OO328_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5932389.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          643 a          618 c          655 g          713 t
ORIGIN      
        1 gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac gttttattaa
       61 acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag aacaaatccc
      121 tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca tgaaataacc
      181 ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga tttttggctg
      241 gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc tattctcggt
      301 gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc cggattaacc
      361 cctgcaggtg gtgttcagcc gcccaacccg attatggcag gtctgatgac caccgtcatt
      421 gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc gtttagtttg
      481 ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat gaccaaagcc
      541 gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg gacaacgatg
      601 ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct ggcacaaggg
      661 gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt tgaagtggct
      721 ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt caaagcgcaa
      781 tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt cagcaacctg
      841 ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa tgcgaaatcc
      901 cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga ctacagcaat
      961 gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt cgctgctgca
     1021 agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga tgttgccgat
     1081 cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga aagataacct
     1141 cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat taatttcgat
     1201 ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac tgtttggccc
     1261 aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta tggcgggaat
     1321 ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt tcgccgttta
     1381 cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag tcggcgtgaa
     1441 ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa ccatcctcgg
     1501 gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg tgacatcgtt
     1561 tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata aagtcttccc
     1621 gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg tgaaaaaagc
     1681 gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt ccgcattcgg
     1741 cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta aggaatttca
     1801 tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac gagccagcga
     1861 atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg cgaccggagc
     1921 cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc aggttgatat
     1981 cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa cgatgcctgg
     2041 cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac gtgaagacca
     2101 gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg taacgaacct
     2161 gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa acggtcatct
     2221 tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc aacttgatgc
     2281 cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc gtgggatcac
     2341 cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga ctggcgaagg
     2401 aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta tcccggcttc
     2461 atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag atgaaaaatt
     2521 tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa taacatttga
     2581 ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattt
//
LOCUS       DAJTRN010000219         2593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTRN010000219 DAJTRN010000000
VERSION     DAJTRN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            50..2572
                     /locus_tag="OO328_004050"
     CDS             50..2572
                     /locus_tag="OO328_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5932390.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
BASE COUNT          701 a          560 c          651 g          681 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gta
//
LOCUS       DAJTRN010000220         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRN010000220 DAJTRN010000000
VERSION     DAJTRN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(64..822)
                     /locus_tag="OO328_004051"
     CDS             complement(64..822)
                     /locus_tag="OO328_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5932391.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO328_004052"
     CDS             complement(830..1933)
                     /locus_tag="OO328_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5932392.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO328_004053"
     CDS             complement(1943..>2572)
                     /locus_tag="OO328_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5932393.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTRN010000221         2565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTRN010000221 DAJTRN010000000
VERSION     DAJTRN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            108..2105
                     /gene="betT"
                     /locus_tag="OO328_004054"
     CDS             108..2105
                     /gene="betT"
                     /locus_tag="OO328_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5932394.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..>2565
                     /locus_tag="OO328_004055"
     CDS             2168..>2565
                     /locus_tag="OO328_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5932395.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRV"
BASE COUNT          580 a          596 c          664 g          725 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagt
//
LOCUS       DAJTRN010000222         2542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTRN010000222 DAJTRN010000000
VERSION     DAJTRN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..161
                     /locus_tag="OO328_004056"
     CDS             <1..161
                     /locus_tag="OO328_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5932396.1"
                     /translation="QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFID
                     RLKYKGAV"
     gene            498..2111
                     /gene="mppA"
                     /locus_tag="OO328_004057"
     CDS             498..2111
                     /gene="mppA"
                     /locus_tag="OO328_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5932397.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2162..>2542)
                     /gene="ynaI"
                     /locus_tag="OO328_004058"
     CDS             complement(2162..>2542)
                     /gene="ynaI"
                     /locus_tag="OO328_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5932398.1"
                     /translation="NSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREM
                     LKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQS
                     HGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          701 a          557 c          605 g          679 t
ORIGIN      
        1 atcaggttga gcgggctatt aaagaaaaaa aactgatacg tgttttggat aaattcactc
       61 cagatttacc cggttatcac ctgtactatc cacaccgtcg acatgctggc tcggcattct
      121 cattatttat agataggctg aagtataaag gtgctgttta gcactacttg ctgatacatt
      181 aatttaattc ttctcttaac gtattctcag ttcctttcaa cgttttggtt attttttatt
      241 cttcgtacaa tggcgacaga tgctgattat gataccgaaa actggtttga acgtgcgaag
      301 cccgaacgta gtgttcggtg ttctatgtgc tttgccacat tttggcgtct attatttaca
      361 ctaaatctga tttgatatat tgatacttaa aacatttgat gcttcctttg tcactttttt
      421 gatggaagtt gtttgcattt ctttaaggcg aaacaaataa ttacgcatca attttaatgt
      481 cggttagagg gaaacttatg aagcactctg tttcagttac gtgttgtgcg ctgttggtca
      541 gcagcatttc tctttcgtat gctgcagaag ttccgagcgg cacagtactg gcagagaagc
      601 aggagctggt gcgccacatt aaagatgagc ctgcgtcgct ggatcccgct aaagccgtgg
      661 gcctgccaga gattcaggtc attcgcgatc tgtttgaagg tctggtgaat cagaacgaaa
      721 aaggggagat tgtccccggc gtcgcgactc aatggaaaag taatgacaac cgtatctgga
      781 cttttaccct gcgcgataac gcaaaatggg cggatggcac accggtaacg gcgcaagatt
      841 ttgtctacag ctggcaacgt ctggtggacc caaaaacatt gtcgccattt gcatggtttg
      901 ccgcgctggc gggaatcaac aacgcacagg cgattattga tggtaaagct acgcctgatc
      961 agcttggcgt caccgcagtt gatgcccata ctttgaaaat tcagcttgat aaaccgttgc
     1021 cgtggtttgt gaatttaacc gctaactttg ccttcttccc ggtgcaaaaa gccaacgtag
     1081 aaagcggtaa agagtggacg aaacccggaa atctgatcgg caatggcgct tatgttctta
     1141 aagagcgcgt agtcaatgaa aaactggtcg tggtaccgaa tacccattat tgggataacg
     1201 ccaaaacggt gctgcaaaaa gtgaccttcc tgccaattaa tcaggaatcc gcagccacta
     1261 agcgttacct cgcgggggat attgatatca ccgaatcctt cccgaaaaat atgtatcaga
     1321 agctgttgaa ggatattccg gggcaggttt atacgccacc gcagctcggg acctattatt
     1381 atgcctttaa cacgcaaaaa gggccgacgg cagatcagcg cgttcgcctg gcattaagta
     1441 tgacgataga tcgccgcctg atgaccgaaa aagtgttggg gacgggcgaa aagccagcgt
     1501 ggcattttat accagatgtt accgccggat ttacgccgga accttcgccg tttgaacaaa
     1561 tgagtcagga agaactgaat gcgcaggcaa aaactttgtt gagcgcagct ggttatggtc
     1621 cgcaaaaacc gctgaagctg acgcttttgt ataacacttc agaaaaccat caaaaaattg
     1681 cgattgctgt agcatcgatg tggaaaaaga accttggcgt agatgttaaa ttgcaaaatc
     1741 aggaatggaa aacctatatc gatagccgta acaccggcaa ttttgatgtt atccgcgcct
     1801 catgggtggg ggattataat gaaccctcca ctttcctgac attattaacg tcaatgcatt
     1861 caggaaatat ttcacgcttt aacaatccgg catacgacaa agttctggcc caggcatcga
     1921 cggaaaatac cgttaaagcg cgtaatgccg attacaacgc ggcagaaaaa atcctcatgg
     1981 agcaagcacc gattgcacca atttatcaat ataccaatgg acgattaatc aaaccgtggc
     2041 tgaaaggtta tcccattaat aaccctgaag atgtagcgta cagtcggact atgtatattg
     2101 tgaagcattg atgtgatggg aactggcgtt acccttgtgg ataacgccag tgatgtctga
     2161 tttagcgccc ctgttccggc ggtgtaatgt tatccatata cagcgtctgg ctggggaagg
     2221 caaagtccgc gccgtgtgac tgtacaatat cgataatctt caaataaacg tcttgctgtg
     2281 cagcaagcca ctcagcccat accgtggttt tggtaaagca ataaaccata atattcaatg
     2341 acgagtcagc aaactggttg aaataaacca gtaaggtttg tcgctggtcg atggccgggt
     2401 gatttttcag catctcacgt acggcttcga caataacgcc cacttttgcc gcatcctcat
     2461 aacgtaaacc aatggtcgtg gtaatgcggc ggttggtcat tcgtcctggg ttttctacgc
     2521 tgatcgacga aaacagcgag tt
//
LOCUS       DAJTRN010000223         2521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRN010000223 DAJTRN010000000
VERSION     DAJTRN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..430
                     /locus_tag="OO328_004059"
     CDS             <1..430
                     /locus_tag="OO328_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932399.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="OO328_004060"
     CDS             550..723
                     /locus_tag="OO328_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5932400.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="OO328_004061"
     CDS             823..1644
                     /locus_tag="OO328_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5932401.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            1986..2459
                     /locus_tag="OO328_004062"
     CDS             1986..2459
                     /locus_tag="OO328_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5932402.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT          618 a          665 c          653 g          585 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctgaggcagc atgtccctga aggcgtgatt caggcaccag
     1681 aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat gatgacctgt
     1741 gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc agactgagta
     1801 tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt acggctgtcc
     1861 ggaatttcag ccgaatatca ttcatccctg atattaccgg gtcatcacgg tcagtgatga
     1921 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
     1981 catacatgaa aacattgtct cagaacacca cctcttcggc ctgtgcaccg gagactgacc
     2041 tgcaacaact ggttgccacc ctcgtccctg atgaacagcg catcagcttc tggccgcagc
     2101 actttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
     2161 gtctgtgcga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     2221 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     2281 tgaacggtaa ccgcgctgaa atgagtccgg aagctgccgg cattgctgcc tgtctgatga
     2341 cgtacagtca tcatgcctgt cgtacggagt gttatgccat gacggtccat tattaccggc
     2401 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     2461 tggtggcagg gacaatgcag cagctttcct ttctgcccgg agaaatgaca accagagagc
     2521 g
//
LOCUS       DAJTRN010000224         2489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRN010000224 DAJTRN010000000
VERSION     DAJTRN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1174
                     /gene="tet(B)"
                     /locus_tag="OO328_004063"
     CDS             <1..1174
                     /gene="tet(B)"
                     /locus_tag="OO328_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5932403.1"
                     /translation="TLLDAMGIGLIMPVLPTLLREFIASEDIANHFGVLLALYALMQV
                     IFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLSGITGATGAV
                     AASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFIAALLNIVA
                     FLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQLIGQIPAT
                     VWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLGFIADSS
                     AFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVSLTNAT
                     GVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQETSA"
     gene            complement(1287..1880)
                     /gene="tetC"
                     /locus_tag="OO328_004064"
     CDS             complement(1287..1880)
                     /gene="tetC"
                     /locus_tag="OO328_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5932404.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2013..2384
                     /locus_tag="OO328_004065"
     CDS             2013..2384
                     /locus_tag="OO328_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932405.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          657 a          438 c          531 g          863 t
ORIGIN      
        1 tacgttactc gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg
       61 tgaatttatt gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc
      121 gttaatgcag gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg
      181 cccagtgctg ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc
      241 aagtgcgctt tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg
      301 ggctgtcgcg gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt
      361 cggttggtta ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt
      421 tgcaggagag atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc
      481 tttccttgtg gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac
      541 cgaagtaggg gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc
      601 cattttgttg attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg
      661 ggtgctattt accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc
      721 gggtcttggt cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa
      781 atggggcgaa aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt
      841 tttagcgttt atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg
      901 tgggatcgct ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca
      961 aggtgcttta cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt
     1021 actgtttgct gttatttata atcattcact accaatttgg gatggctgga tttggattat
     1081 tggtttagcg ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca
     1141 agctcagggg agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt
     1201 ccgcgaaata taatgaccct cttgataacc caagagggca ttttttacga taaagaagat
     1261 ttagcttcaa ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg
     1321 taaatagcaa taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt
     1381 gcgatatgca taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta
     1441 agttggttct cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca
     1501 aggctaattt tttcaagttc attccaacca atgataggca tcacttcttg gatagggata
     1561 aggtttttat tattatcaat aatataatca agataatgtt caaatatact ttctaaggca
     1621 gaccaaccat ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc
     1681 tgcttataac aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct
     1741 ttggtcacca acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca
     1801 acaaacaata ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt
     1861 tgatgatttt tattttccat gatagattta aaataacata ccgtcagtat gtttatggta
     1921 tcatgatgat gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac
     1981 agcattctcg ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt
     2041 ttcatacaac ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt
     2101 tactgcttga tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt
     2161 tcaaaaaagt aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag
     2221 cggctgttga gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat
     2281 ttgattccca acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt
     2341 attatcggcg taataaatta tgggaattgg aggcaatgca ctgagagggt gatgctgcca
     2401 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
     2461 agctgtccct cctgttcagc tactgacgg
//
LOCUS       DAJTRN010000225         2435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRN010000225 DAJTRN010000000
VERSION     DAJTRN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(38..583)
                     /locus_tag="OO328_004066"
                     /pseudo
     CDS             complement(38..583)
                     /locus_tag="OO328_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1245..2234)
                     /locus_tag="OO328_004067"
     CDS             complement(1245..2234)
                     /locus_tag="OO328_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932406.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          652 a          556 c          584 g          643 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctgag ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg agcctacgac aaatcgaaga
      841 caaccagcta acctattgtt tcttaaagac aggtgaatcc agttggttgt agtgatagtc
      901 acacatttag agatagaagg tcaagacttg aagcacgtaa tattccctct aaaggaatag
      961 aactcacggt ctgactaccc ggcccttttg gtctggctca ggtcaggcta tgggggcttt
     1021 tggctcgctc tttatattag atgaggtctc acatttttca gtcaaaactc atcgtctaat
     1081 agcccatagc cagaccacca gtcccagctt tagcaggaag accacgagat agactttgtg
     1141 taggtccatt tggccctcct actttctgta ttcacaaact agattactag tacaaaataa
     1201 aggccagaca tccagttcgg aagtctgacc aatagtcgtt attgttagtt agcttcttta
     1261 gtgaagctca ggcgtttgac tacaggcagg acagcagtag ccgagccagt acccatcttg
     1321 atggtcgtag cagcttcagc tacaacaaac accttgccga gaatctcaag gtcgtgagac
     1381 tgggagtctc catcacgtga aaggaccaca gtaatgctct cgccatcgtg acggaagcaa
     1441 ccgaagctca gagccgtaaa acgcttagcc atcatgaaca cgccacggtc ttcaccgttg
     1501 taataggcga cgtcagtccc cagcatatcg acgatagcgt cttcaccacc gaagccctgc
     1561 ttagcagcca gacgggagag agccttgagg tgtgactcat gggcgataat gccgtagtca
     1621 gaaacctcag aaccgaaaga agactccgtg ttctcgtaca gggcatccat gatagcgtca
     1681 gccttagcgg tcaggctacc agtcagggcc ggaagttcta cggtcttcag cagctttgag
     1741 gactccagca cgtcaaccat cttagagtta atggtacgaa tcacgttatc gcagatgttc
     1801 agtgcgtcct gctggagcgt cccctgagtg aaaccagtgg tcgacttctg gtcccaaagc
     1861 tttgtttggt gagacacgag gatcgcctgt tcaatcggct caacggtgat ttcagtgcca
     1921 gacggagcgg tcacaacttc aggtcgctta ccaccacgga cagggaccag cacagagcgg
     1981 tcagccagag tgtgagcaat gattggagtc ggtaagctgt tacgggtcgg caggacttct
     2041 acagggaacg ggatgttatc ttgagcgtct ttaagtacac cgttcagcat agccttaaca
     2101 gtcttagcta actggagggc atagacctga gggtcggcgt tagtcggcag accttcattg
     2161 acctgaatgt tgtcggtatc ggtgatgcgt actggctcaa tgccaaactg gaaagcgttt
     2221 ttagtcatat tcattataat taatctcctt gagtcaaata cttgaagtcc ctaacaggca
     2281 gtcagggacc gtgtgttctc agatgaggga cgcttgctta caagctgtcc atccagttac
     2341 cgcctgactc aaggtcagcg acgatagcgg cacgtgagcg accagcggaa tgagtagcgc
     2401 tgcggaggtt ttcaccatag gtccggcgtt cagct
//
LOCUS       DAJTRN010000226         2328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRN010000226 DAJTRN010000000
VERSION     DAJTRN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1015
                     /gene="ccp"
                     /locus_tag="OO328_004068"
     CDS             <1..1015
                     /gene="ccp"
                     /locus_tag="OO328_004068"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5932407.1"
                     /translation="RYTALHWAGKVSDEERAEILAWIAKQRAEYYASNDTAPEHRNEP
                     VQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGVDGRKTSIGVGGA
                     VGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSWDEIIAKLEKDPQ
                     LKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENALTAQQKKGYQLF
                     KDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKEERDKLRQKVPGL
                     RNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQ
                     DKQ"
     gene            1226..>2328
                     /locus_tag="OO328_004069"
     CDS             1226..>2328
                     /locus_tag="OO328_004069"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5932408.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          592 a          532 c          619 g          585 t
ORIGIN      
        1 gcgttatacc gcgctacact gggcgggtaa ggtgagtgat gaagagcggg cggaaatact
       61 ggcctggatt gcaaaacagc gcgcggaata ttacgccagc aatgatactg ctccggagca
      121 tcgcaatgaa ccggtgcagc ccatcccgca aaaactgcct accgatgcgc aaaaagtggc
      181 gttgggcttt gcgctgtatc acgatccccg tttatcggct gatagcacca tttcatgcgc
      241 tcattgccat acgttgaatg cggggggcgt cgatggcaga aaaacatcga ttggtgttgg
      301 tggcgcagtt gggccgatta atgcgccgac ggtatttaac tcagtattta acgttgagca
      361 gttctgggat ggtcgtgcgg caacattgca ggatcaggca ggtggaccgc cgttgaaccc
      421 gattgaaatg gcgtcgaaat cctgggacga aattattgct aagctggaaa aagatccgca
      481 gcttaaagcg cagttcctcg aagtttatcc gcaaggtttc agtggcgaaa atattactga
      541 tgccattgct gaatttgaga aaacattaat tacgccggat tccccatttg ataaatggtt
      601 gcgtggagat gaaaatgcgc tgacggcgca acagaaaaaa ggctatcaat tatttaaaga
      661 taataaatgt gcaacttgtc atggtggtat tattctcggc ggacgttcct ttgaaccgtt
      721 ggggctgaaa aaagacttta actttgggga aattacggcg gcggatattg gtcgtatgaa
      781 tgtgactaaa gaagagcgtg ataaattgcg tcagaaagta cccggtttac gtaacgttgc
      841 tttaacggca ccgtacttcc atcgcggtga cgtgccgacg ctggacgggg cggtgaaact
      901 gatgctgcgc tatcaggtag gcaaagagct gccgcaggag gatgtggatg atatcgtagc
      961 tttcctgcac agtctgaacg gggtgtacac gccgtatatg caggataaac aataattaat
     1021 ttgatcgccc gaacaggaat gtttgggcga tttttattac gataataaag tctgttttta
     1081 atattatcat gttaaatgtt tatattataa aaagtcgttt ttctgcttag gattttgtta
     1141 tttaaattaa gcctgtaatg ccttgcttcc attgcggata aatcctactt ttttattgcc
     1201 ttcaaataaa tttaaggagt tcgaaatgga ccagaagctg ttaacggatt tccgctcaga
     1261 actactcgat tcacgttttg gcgcaaaggc catttctact atcgcggagt caaaacgatt
     1321 tccgctgcac gaaatgcgcg atgatgtcgc attccagatt atcaatgatg aattatatct
     1381 tgatggcaac gctcgtcaga acctagccac tttctgccag acctgggacg acgaaaacgt
     1441 ccataaattg atggatttgt cgatcaataa aaactggatc gacaaagaag aatatccgca
     1501 atccgcagcc atcgacctgc gttgcgtaaa tatggttgcc gatctgtggc atgcgcctgc
     1561 gccgaaaaat ggtcaggccg ttggcaccaa caccattggt tcttccgagg cctgtatgct
     1621 cggcgggatg gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag gcaaaccaac
     1681 ggataaacca aacctagtgt gcggtccggt acaaatctgc tggcataaat tcgcccgcta
     1741 ctgggatgtg gagctgcgtg agatccctat gcgccccggt cagttgttta tggacccgaa
     1801 acgcatgatt gaagcctgtg acgaaaacac catcggcgtg gtgccgactt tcggcgtgac
     1861 ctacaccggt aactatgagt tcccacaacc gctgcacgat gcgctggata aattccaggc
     1921 cgacaccggt atcgacatcg acatgcacat cgacgctgcc agcggtggct tcctggcacc
     1981 gttcgtcgcc ccggatatcg tctgggactt ccgcctgccg cgtgtgaaat cgatcagtgc
     2041 ttcaggccat aaattcggtc tggctccgct gggctgcggc tgggttatct ggcgtgacga
     2101 agaagcgctg ccgcaggaac tggtgttcaa cgttgactac ctgggtggtc aaattggtac
     2161 ttttgccatc aacttctccc gcccggcggg tcaggtaatt gcacagtact atgaattcct
     2221 gcgcctcggt cgtgaaggct ataccaaagt acagaacgcc tcttaccagg ttgccgctta
     2281 tctggcggat gaaatcgcca aacaggggcc gtatgagttc atctgtac
//
LOCUS       DAJTRN010000227         2281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTRN010000227 DAJTRN010000000
VERSION     DAJTRN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..798
                     /locus_tag="OO328_004070"
                     /pseudo
     CDS             1..798
                     /locus_tag="OO328_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            913..1254
                     /locus_tag="OO328_004071"
                     /pseudo
     CDS             913..1254
                     /locus_tag="OO328_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1237..2074
                     /locus_tag="OO328_004072"
                     /pseudo
     CDS             1237..2074
                     /locus_tag="OO328_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2124..>2281)
                     /locus_tag="OO328_004073"
     CDS             complement(2124..>2281)
                     /locus_tag="OO328_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5932409.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          553 a          555 c          692 g          481 t
ORIGIN      
        1 ctgggccttg cccgtagcag ttatttttat caccgggctg cactgaaagc cggtgataaa
       61 tacgccacga tacgtaagat gctgacagat atctttaaca gtaattacca gtgttatggc
      121 tatcgtcgcc tgcatgcgat gctcaggcat gagggggggc ggctatcaga aaaggttgta
      181 cgcagactta tggtggagga acagcttgtc gtcagtcgta accgtcgtcg tcgctacagc
      241 tcatattgcg gagaaatcgg accggctccg gataacctta tcgccagaga ttttaaggcg
      301 gagcaaccta atcagaaata gctgacagat atcacggagt tccagctccc tgcaggtaaa
      361 gtctggctgt caccagtggt ggactgcttc gatggaaaag ttgtgagctg gtctctcagt
      421 acacgccctg atgctgaact ggtcaacact atgctggata gcgctgtcga aacgttaaat
      481 gctggcgaac gaccggtgat acacagtgac agaggtgggc attatcgctg gccaggctgg
      541 ctggaaagag tgaatgcagc aggtcttatt cgctcaatgt cccgtaaagg atgttcacct
      601 gataatgctg catgcgaagg ctttttcggc agactgaaaa cggaaatgta ttatgggcgt
      661 aaatggtcgg gcatcacgcc agaaaagttc atgcagcaag tggatgctta catcagatgg
      721 tataacgagc ggcgtataaa attatcgctg ggtgccgtca gccccaaaat gtaccgccaa
      781 caatgcgggc tggaatgata aagcagtcca ggaaatcgtc cgcatccccc cttcaccatt
      841 tggattgtcc gctccaccca acatgttgtt tccttaaggt tcctacacca caaaggaaac
      901 tctcatgctg cgcagtggat ggctgacgtt gccgacaggc gggaacttcg ccagttcagg
      961 cagacaccgg aacagcgctt cacgcaggaa caggagcatc tgcagccgct gctgggtacg
     1021 gacttcgata tccgtcatgt ctcctgggat ggctatatcg aggttggcgg gaaccgttac
     1081 agtgtgccgg aaagcctgtg tggtcagctg gtatcaatac ggatctcgct ggatgatgaa
     1141 ctgcggatct acagtaatga gcaacaggta gcatcgcacc gtctctgttc ggctgcatac
     1201 ggctggcaga ccgtgccgga gcatcacgct ccactctggc agcaggcttc ggagcgcatg
     1261 gccgaatggc tgggggagat acagaaacgg gtaatcactg tctgtgaccg ggaagcggat
     1321 atctggcatt acctgtatta taaagtcagc cacggacagc gtggtgcgtg ctgcacagaa
     1381 tcgccggctg gaagaggcac ccggcaagct cttcgaactg ccggaagtcc tggcaaccgc
     1441 aggaagccac acgctgaatg tgatgcaaaa aggcgggcgg gcagcccgtc aggcccggat
     1501 gttcatcagt tacagcgaag tcagcataaa aaatcccgac aacagcggcc aggcgctccc
     1561 gctcacgtat gtctgttgcc gggagcaggc agaggacggt gcctgctggc atctgctgac
     1621 gtcaggaaaa gcggcgagtg ccgccgatgc acgacgtatt gtcagccatt acgagcgacg
     1681 ctggctgaca gaggaatacc acaaggcgtg gaaaagtggt ggtacatgga atcgctgcga
     1741 atgcagaccc gggataacct tgagcgcatg gtggttatcc tggcgtttat agcggtacgg
     1801 gtgctgggcc tgcggcaggg aggtgtcagt gaagaaacac aaaatgacag ctgtgagaaa
     1861 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gccgcttccg
     1921 gtgcaggcac cgaccctgaa atgggcctgg ggcgatggca tgacagcaaa cgcacaggtc
     1981 gtcccggttg gggcgtcatg tgggatggct ggtcagactt caggatatgg ttgagggtta
     2041 cctggtgatg aagtctcttg atcaggagct ctgatcaaga gacagcagct taggggggtg
     2101 cccctattaa gttgtcaagc atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc
     2161 atcatggcga acagaacgtc gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt
     2221 cgttttccct gactcatttt gcgggtgtag taagccctgg agagcggatc cctgagcgcg
     2281 g
//
LOCUS       DAJTRN010000228         2240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRN010000228 DAJTRN010000000
VERSION     DAJTRN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            661..2169
                     /gene="ltrA"
                     /locus_tag="OO328_004074"
     CDS             661..2169
                     /gene="ltrA"
                     /locus_tag="OO328_004074"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5932410.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          573 a          550 c          662 g          455 t
ORIGIN      
        1 gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt
       61 aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca
      121 gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga
      181 tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt
      241 taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca
      301 ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga
      361 aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa
      421 gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac
      481 cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg
      541 agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga
      601 atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa
      661 atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg
      721 ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca
      781 aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg
      841 gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc
      901 gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg
      961 ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt
     1021 gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc
     1081 acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc
     1141 gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc
     1201 aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc
     1261 gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg
     1321 ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc
     1381 gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg
     1441 gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat
     1501 tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg
     1561 ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat
     1621 gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc
     1681 gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg
     1741 acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac
     1801 cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc
     1861 agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt
     1921 acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa
     1981 acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg
     2041 ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg
     2101 aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc
     2161 agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg
     2221 cagggaaata gtccgactac
//
LOCUS       DAJTRN010000229         2191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRN010000229 DAJTRN010000000
VERSION     DAJTRN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            52..1038
                     /locus_tag="OO328_004075"
     CDS             52..1038
                     /locus_tag="OO328_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5932411.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVS
                     IAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            1039..1404
                     /locus_tag="OO328_004076"
     CDS             1039..1404
                     /locus_tag="OO328_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5932412.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            1401..2084
                     /locus_tag="OO328_004077"
     CDS             1401..2084
                     /locus_tag="OO328_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5932413.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          582 a          501 c          604 g          504 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcctcac
      661 gcccaccagc aacagaatcc accacaggaa gagacggtgg tcagcattgc cgttgatccg
      721 gaatctccgg ctcagtatct ccagcgccag aaaccacaac gggaagagat gcctgtatac
      781 acgcgctggg taaaaacgca gaaatgcatg acgtgtggca atcaggcaga tgatccgcac
      841 cacatcattg gtcatggact gggagggatg ggaacaaagg ctgacgattt gtttgttatt
      901 ccgctgtgtc gtaaatgcca taacgaacta cacgccgggg taaaagattt tgaagaaaaa
      961 cacggtagtc agttgttgtt gctgattcgt tttttaatgc acgcgagaaa ttcgggtgtt
     1021 ctgaagtgga aagcataaat gaccgaacgc atagaatttg ttttgcctta cccgccaacg
     1081 gtgaacacct actggcgacg tcgtggcagc acatattttg tatcaaaagc cggtgagcgt
     1141 tatcgccgtg atgtggcgct aattgttcgc cagcagcgac tgaaattaaa cctgtccgga
     1201 aggctggcga taaagattat tgcagagcca ccggataagc gccgtcgtga cctggacaat
     1261 atcctgaagg caccgctgga tgcgctgacg catgctggac tacttataga cgacgagcag
     1321 tttgatgaaa tcaatattgt gcgcggtcag ctcgttcctg gtgggcggct ggggataaaa
     1381 atcacagaac tggggtgcgc atgaataacc agtatttaca gtttgtgcgt gagcagctca
     1441 ttatcgccac cgctgatttg agtggggcaa caaaaggtca gcttgaagcc tggcaagaga
     1501 atgccatgtt cgatacaggg cgttacaggc gtaaaaaaat ccggtaccgc gatgaagtga
     1561 ctggaaaaat gataacgcgg gataatccac caatcccggg aaagcaatcg ctggcgaagg
     1621 ggacgtcaat tcctctggtc agtccggttg agttttcgac atcatcgtgg cggcgggctg
     1681 ttctgtctct tgaagagcac tataaagcct ggttgttgtg gtgttacagc gggagtattt
     1741 gttgggaata tcagatcgcg ataacacagt gggcgtggaa tgaatttaat gctcaatccg
     1801 gtaccagaaa aattgcaggg aaaacgcagg aacgcctgaa aaaattaatc tggctggcgg
     1861 cgcaggcagt aaaagcagaa ctttttggtg gggaaggtta tgaataccag gagctggcat
     1921 tactggcggg agtgacaact aaaaactggt ccaaaacatt tactcgtcac tgggttgcaa
     1981 tgaaacacat ttttcaccga ctggatagtg aggctttatt gtttgtaatg agaacacgtt
     2041 caaaaaaaag acggcatttt caaagcaaag tgttgcaaaa gtagattgaa aggcatatat
     2101 tcatgcaaat ctgatacttt gccgattttg tacgtgatgg caaaagcaaa caaaacccgc
     2161 ccacaagcgg gttttttgtg ccacttatct c
//
LOCUS       DAJTRN010000230         2162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRN010000230 DAJTRN010000000
VERSION     DAJTRN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            80..259
                     /locus_tag="OO328_004078"
     CDS             80..259
                     /locus_tag="OO328_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932414.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO328_004079"
     CDS             311..505
                     /locus_tag="OO328_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932415.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="OO328_004080"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="OO328_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAJTRN010000231         2112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRN010000231 DAJTRN010000000
VERSION     DAJTRN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            27..317
                     /gene="lsrG"
                     /locus_tag="OO328_004081"
     CDS             27..317
                     /gene="lsrG"
                     /locus_tag="OO328_004081"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5932416.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            357..1115
                     /gene="tam"
                     /locus_tag="OO328_004082"
     CDS             357..1115
                     /gene="tam"
                     /locus_tag="OO328_004082"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5932417.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1119..2033)
                     /locus_tag="OO328_004083"
     CDS             complement(1119..2033)
                     /locus_tag="OO328_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5932418.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEEPEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          569 a          504 c          542 g          497 t
ORIGIN      
        1 taactgcgga tttaaggaga agaattatgc acgtcacact ggttgaaatt aacgttcatg
       61 aagacaaggt tgacgagttt atcgaagttt ttcgccagaa ccacctgggc tctgtacagg
      121 aagaaggtaa tttgcgcttc gatgtcttac aggacccgga agtgaattcg cgcttttata
      181 tttacgaagc ctataaagat gaagacacag tggcattcca taaaaccacg cctcactaca
      241 aaacctgtgt cgcgaaactg gaatctttaa tgaccgggcc gcgtaaaaaa cgtctgttca
      301 atgggttgat gccgtgaggc tacaattggg gtaacgcgct gacgggagta aaaaaaatgt
      361 ctgactggaa cccctcttta tatctgcact tttccgctga acgatcgcgt ccggcggtgg
      421 agctgcttgc cagagtgccg ctggagaatg tcgaatatgt tgccgatctt ggctgtggcc
      481 caggtaacag caccgccctg ctgcatcaac gttggcctgc ggccaggata acaggcatcg
      541 actcgtctcc ggcgatgatt gctgaagcgc gcagtgcttt gccagactgc cagttcgtgg
      601 aagcagacat ccgcaactgg caaccggaac aggcactcga tctgattttt gctaatgcct
      661 cactgcaatg gctgcccgac cactacgaat tgtttcctca cttggtttct ttacttaatc
      721 cgcagggcgt gctggcagta cagatgccag ataactggct ggagccgacc catgtgctca
      781 tgcgcgaagt tgcttgggaa caaaactacc cggatcgcgg gcgtgagccg ttggctggcg
      841 ttcatgctta ctacgatatt ttgagcgaag ccggatgtga ggtcgatatc tggcgaacca
      901 cttactatca ccagatgccg tcgcaccagg cgattatcga ttgggtgacc gccactggat
      961 tacgtccgtg gttacaggat ctgaccgaga gcgaacagca gctttttctt acgcgctacc
     1021 atcagatgct ggaagagcag tatccactgc aagagaacgg acagatactg ctggcatttc
     1081 cgcgtctgtt tattgttgcc cgccgtacgg agtaattatc acgtcagctg gtaatgacga
     1141 tcgggaagaa tttttgctgg aatttctgct tcatcgttca tctgtaacag gtcaatttca
     1201 atagcgttgc agatggcatc cagcggtaaa tcattgtttt cagtaccgaa cggatcttcc
     1261 ggttcttccg ccagacaatc cagcgaaata aaagtgtagg aaatcagcac agagataaaa
     1321 ggcgtcatgt aatgcaggtc cacgaccagc gcgaacggca gcatgataca aaacagataa
     1381 acggtacgat gcaaaatcag ggtgtaggca aagggaattg gcgtgtaggc aatgcgctca
     1441 cagcctgcca gaaccgctga aatatcatta agccgatcgt tgaggctaat aaacaggata
     1501 tctgaaagct gtccattgcg gcgctgaacc gccaaccatt ctcccattat taacaagata
     1561 cggttagctg gagagttcga agccagtaca cgctgaagat ctccagtctt gagataatga
     1621 gccagcactt ccgcctgtgg ctgtttgcgt aatgtcatgc gtaaacagtg ggcgaaggcg
     1681 atttgcagcc tggcaaactc ccttacactt gccgaatccg gcaatgtcgt ttttacctct
     1741 cgcagtaacg accgtgaggc aatcatcaac tgcccccaaa gttttcgcgc ttcaacgtaa
     1801 cgggcgtacc cggcattatt acgaaaacca agaaaaatgg cgatggcgac accgagaatg
     1861 ctgaacggtg cgagggtgaa tttgatgccc agatgcgtgt accagggcag catgaatata
     1921 acagcaatag aaaagagaaa attgagtagt aagcgcgagg atatcttgga taatactgag
     1981 ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg gacgaacaat catggttatc
     2041 ttcaggcgtg gaaaagtcgc cctattaaac gtgattacga tcacattctc aagacgctac
     2101 ttacaaatta cc
//
LOCUS       DAJTRN010000232         2109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRN010000232 DAJTRN010000000
VERSION     DAJTRN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..216
                     /locus_tag="OO328_004084"
     CDS             <1..216
                     /locus_tag="OO328_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR5932419.1"
                     /translation="INQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            174..851
                     /gene="bglJ"
                     /locus_tag="OO328_004085"
     CDS             174..851
                     /gene="bglJ"
                     /locus_tag="OO328_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5932420.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(889..1677)
                     /gene="fhuF"
                     /locus_tag="OO328_004086"
     CDS             complement(889..1677)
                     /gene="fhuF"
                     /locus_tag="OO328_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5932421.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            1818..2054
                     /locus_tag="OO328_004087"
     CDS             1818..2054
                     /locus_tag="OO328_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5932422.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          550 a          452 c          556 g          551 t
ORIGIN      
        1 atcaaccaga tagcttcatt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac
       61 agtgcgatgc gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc
      121 gcgcagggtg caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac
      181 acagccgaat taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agtagtatcg
      241 gtattgagag tttattcaga aagtttgcgg gcaaccctta taagctccat acctatacca
      301 gtcaggagtc atttcaggat gccatgtcgc ggatctcgtt tgcggcggtc attttttctt
      361 tttctgccat gagaagtgag cgcagagagg gattatcttg cctgactgag ctggcgatta
      421 agtttccgcg tacccggcgt ttagttattg tggatgatga tattgaagct cggctgattg
      481 gttcattgtc gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt
      541 ttcatcagga actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca
      601 atcagtggta cattaaccaa agccggacgt taagcccgac ggagagagaa atattgcgct
      661 ttatgtcgcg tggctactca atgacacaaa ttgccgagca gcttaaacgt aatatcaaaa
      721 cgatccgagc gcataaattt aatgtgatgt cgaaactggg cgtcagttct gatgcagggt
      781 tgttggaggc cgaagatatt ctgttatgta tgcggcattg cgaagcaagt aatgtgttgc
      841 atccctatta atccgcatga tgccgggttt acttccccgg cagtgctttc atttcagcgt
      901 acaatagcca cattgctgca catccggtaa gcgataacgc tggcagcaag tgcggcgcac
      961 cagcaggccg tcgcgcagta ccacggtacg ccagagtgga ttatcttcac cgttcgtgag
     1021 cgttttctca aaaaagaggg catggcgcag cgattcaaca gtagcctcgc cgagcagttg
     1081 cttcatctca gtgagatacc agttgatcaa ataaccggta ttactccaga taagtttgcc
     1141 gttgatctct ccggtcgctt ctagtgcttg cacaaccgga accagcgcct ggctgattaa
     1201 cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt ttatcttcac acacatcgac
     1261 ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca tggaaatgtt ccggcgacac
     1321 atctaatgct ttttcctgcg tcagtagcgc cagcattaat ggtggcacca tcaggccgat
     1381 ataccattgt gcccatagtg agatcagcgg tttgttctcg cggatcatcg ttggttggtt
     1441 gcgatagata tgatcggaat agaccgccag tagagaactt agcgcattcg gtgatgacca
     1501 ttgcgccagc gtcatggcgt taagcggagc gggttcatcc aggcggataa actccagcaa
     1561 atgttcacga tgtttggcga tcatcgcccg tacggcttgt gcaagcccag catcctgcgg
     1621 ctggagatgc gttcgccaga tgacatcttc atagagcggt gcggaacgat aggccataat
     1681 cgtgatagta atctaaatga taatgattgc taatcctagc gataggttta ctcgatagca
     1741 agggatttct ctggcttgca aatgataaaa attatcatat gatattggtt ttcattatca
     1801 atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag tggctgggga gtgttgctgc
     1861 cgggattgct gattgcaggg ctgatgtatg cggatttatc gccggatcag tggcggattg
     1921 tcattctgat gggattagta ttgacgccgg tgatgctgta tcacaaacag ttgcggcatt
     1981 acattttgct accatcgtgc ctggcgctta ttgctggcat catgctgatg ataatgaatt
     2041 tgaatcaggg atgacaaaac agggaagaaa caagaaagga agtaaagata attggtgcga
     2101 gggggggga
//
LOCUS       DAJTRN010000233         2085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTRN010000233 DAJTRN010000000
VERSION     DAJTRN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..588
                     /locus_tag="OO328_004088"
     CDS             <1..588
                     /locus_tag="OO328_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5932423.1"
                     /translation="GIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGE
                     CGLDMELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTG
                     DVLNDDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGK
                     HSWKSLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            610..1176
                     /gene="yieF"
                     /locus_tag="OO328_004089"
                     /gene_synonym="chrR"
     CDS             610..1176
                     /gene="yieF"
                     /locus_tag="OO328_004089"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5932424.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1230..>2085)
                     /gene="adeP"
                     /locus_tag="OO328_004090"
     CDS             complement(1230..>2085)
                     /gene="adeP"
                     /locus_tag="OO328_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5932425.1"
                     /translation="KNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHA
                     AVLVSIVVTTLLGWMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLV
                     NLFDSSGTLIGVTDKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSS
                     GVSVGGRTGLTAVVVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQ
                     DLTESVPAFITAVMMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFIL
                     KIVFIDAH"
BASE COUNT          562 a          553 c          541 g          429 t
ORIGIN      
        1 ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt tttcagtgat
       61 aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca
      121 accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc
      181 ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat cagtaaacaa
      241 aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt gctaaaacta
      301 accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat tgccggacgc
      361 ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg
      421 tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt agatggcaaa
      481 cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg
      541 atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata gacaaccaca
      601 ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc
      661 tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag catggaagtc
      721 aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca gcaggaagaa
      781 ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg
      841 atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc catcgactgg
      901 ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca gaccagctca
      961 atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct ggttttcctc
     1021 gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa caaagttgat
     1081 ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg gcaattgacc
     1141 gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc agtaatcatg
     1201 catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac aatcttcagg
     1261 ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg cccggtacca
     1321 atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat cgaaaagctg
     1381 aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc ctgccagttc
     1441 acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc agctgcagcg
     1501 tagtctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa cagcagacca
     1561 acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga ggactcaata
     1621 taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat actgtcgaca
     1681 tacagcgcct gcttcatgcg cgggaatttc cccttctcat ccgccagacc tgctttatcg
     1741 gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa agagaaaatc
     1801 acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac aactgtcatt
     1861 acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat ccagcccagc
     1921 agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga ggccagaata
     1981 gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag attaccgatg
     2041 ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttc
//
LOCUS       DAJTRN010000234         2065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRN010000234 DAJTRN010000000
VERSION     DAJTRN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(210..374)
                     /locus_tag="OO328_004091"
     CDS             complement(210..374)
                     /locus_tag="OO328_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932426.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1276..1560
                     /locus_tag="OO328_004092"
     CDS             1276..1560
                     /locus_tag="OO328_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5932427.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1548..2033
                     /locus_tag="OO328_004093"
     CDS             1548..2033
                     /locus_tag="OO328_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5932428.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          533 a          431 c          593 g          508 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      481 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      541 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      601 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      661 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      721 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      781 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      841 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      901 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      961 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
     1021 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1081 aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg ggggcggtgc
     1141 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1201 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1261 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1321 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1381 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1441 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1501 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1561 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc agtgaagctg
     1621 tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga gccacccgga
     1681 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1741 gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1801 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1861 cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat atcggtgtgc
     1921 gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt catcatggtt
     1981 ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa tagggagtaa
     2041 tgccgatcag ttaaggatca gttga
//
LOCUS       DAJTRN010000235         2050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRN010000235 DAJTRN010000000
VERSION     DAJTRN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..158
                     /locus_tag="OO328_004094"
     CDS             <1..158
                     /locus_tag="OO328_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5932429.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
     gene            complement(175..534)
                     /locus_tag="OO328_004095"
                     /pseudo
     CDS             complement(175..534)
                     /locus_tag="OO328_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(534..1454)
                     /locus_tag="OO328_004096"
     CDS             complement(534..1454)
                     /locus_tag="OO328_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5932430.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1592..>2050
                     /gene="pgrR"
                     /locus_tag="OO328_004097"
     CDS             1592..>2050
                     /gene="pgrR"
                     /locus_tag="OO328_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5932431.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          510 a          511 c          482 g          547 t
ORIGIN      
        1 ccgcgctcag ggatccgctc tccagggctt actacacccg caaaatgagt cagggaaaac
       61 gacacaatca ggcgcttatc gccctggcga gacgacgctg cgacgttctg ttcgccatga
      121 tgcgcgacgg gactttttat accccgcagg ggtcataaca tgcttgacaa cttaataggg
      181 gcacccccca cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac
      241 tgcataatcg ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg
      301 aggctaccca ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt
      361 ttcagccaat gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg
      421 atggacgctg ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat
      481 attggcagca atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga
      541 gtttcctcaa agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca
      601 taaagatcca tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc
      661 cgatcgtaca ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc
      721 atcggctgag tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta
      781 aggctgcgca aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa
      841 tactcccagg cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt
      901 ggcataatgg cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca
      961 atgtgtggca gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa
     1021 ccaatattga ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca
     1081 ttggctgtat atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca
     1141 taagaaagcg ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg
     1201 ttttctaact gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata
     1261 gtgacaaatc ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg
     1321 cccccacctg ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga
     1381 gggaaataga tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact
     1441 ttattattca tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat
     1501 cactatagat attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt
     1561 aaatgattaa tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat
     1621 ggcgtttgtc gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat
     1681 ggtgcagtca gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct
     1741 tctgacgcga accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt
     1801 tcttggccct atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa
     1861 ccgcccatcc gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt
     1921 accagccatg cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga
     1981 ttatggtttg accgatgtcg tttctgaacg ttttgatgca ggcgtcctga ggtgtactgg
     2041 caatagcgga
//
LOCUS       DAJTRN010000236         2029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTRN010000236 DAJTRN010000000
VERSION     DAJTRN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..413)
                     /locus_tag="OO328_004098"
     CDS             complement(<1..413)
                     /locus_tag="OO328_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932432.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPW"
     gene            621..1610
                     /gene="ldhA"
                     /locus_tag="OO328_004099"
     CDS             621..1610
                     /gene="ldhA"
                     /locus_tag="OO328_004099"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5932433.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1721..>2029
                     /gene="hslJ"
                     /locus_tag="OO328_004100"
     CDS             1721..>2029
                     /gene="hslJ"
                     /locus_tag="OO328_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5932434.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT          551 a          464 c          510 g          504 t
ORIGIN      
        1 tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcag catggactgc
       61 cactgcgcga gggtttttgg agcagctggc gattgctccg tctgcggcaa tttcgccaga
      121 caagcagaat caagttctac cgtgccgacg ttcaataacc agcggctggg atgtgaaagg
      181 ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag atcgggaatg
      241 attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt cccgagcggt
      301 agccagatgt ccgccagcgt gggaacccac agcccgagcg tcatcagcag cgtcaacggc
      361 acaagaataa tcagtaataa cagcgcgaga acggctttat atttacccag catgggtagt
      421 taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc atgtttaacc
      481 gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt caatatcgcc
      541 atggctttca attatatttg aaattttgta aaatattttt agtagcttaa atgtgattca
      601 acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag tacgacaaga
      661 agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt gactttctgc
      721 tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg
      781 atgacggcag ccacccggtg ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc
      841 tgcgctgtgc cggtttcaat aacgtcgacc ttgatgcggc aaaagaactg gggctgaaag
      901 tagtccgtgt tccagcctat gatccagagg ccgttgctga acacgccatc ggtatgatga
      961 tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct aacttctctc
     1021 tggaaggtct gaccggcttt actatgtatg gcaaaacggc aggcgttatc ggtaccggta
     1081 aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg ctggcgttcg
     1141 atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc
     1201 tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa aactaccatc
     1261 tgttgaacga agccgccttc gatcagatga aaaatggcgt gatgatcgtc aataccagtc
     1321 gcggtgcatt gattgattct caggcggcaa ttgaagcgct gaaaaatcag aaaattggtt
     1381 cgttgggtat ggacgtctat gagaacgaac gcgatctgtt ctttgaagat aaatccaacg
     1441 acgtgatcca ggatgacgta ttccgtcgcc tgtctgcctg ccacaacgtg ctgtttaccg
     1501 ggcaccaggc attcctgaca gcagaagctc tgaccagtat ttctcagact acgctgcaaa
     1561 acttaagcaa tctggaaaaa ggtgaaacct gcccgaacga actggtttaa tcttgcagct
     1621 cccctgcaat ccaggggagc tgattcagat aatccccaat gacctttcat tctctattct
     1681 taaaatagtc ctgagtcaga aactgtaatt gagaaccaca atgaagaaag tagccgcgtt
     1741 tgttgcgcta agcctgctga tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc
     1801 agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag
     1861 cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg
     1921 taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgacagcca aagggctggc
     1981 aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataac
//
LOCUS       DAJTRN010000237         1946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRN010000237 DAJTRN010000000
VERSION     DAJTRN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..491)
                     /locus_tag="OO328_004101"
                     /pseudo
     CDS             complement(1..491)
                     /locus_tag="OO328_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(484..1631)
                     /gene="flhB"
                     /locus_tag="OO328_004102"
                     /pseudo
     CDS             complement(484..1631)
                     /gene="flhB"
                     /locus_tag="OO328_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          518 a          537 c          471 g          420 t
ORIGIN      
        1 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
       61 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
      121 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
      181 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
      241 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
      301 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
      361 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
      421 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
      481 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
      541 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
      601 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
      661 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
      721 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
      781 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
      841 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
      901 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
      961 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     1021 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     1081 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     1141 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     1201 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     1261 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     1321 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     1381 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     1441 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     1501 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     1561 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     1621 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     1681 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     1741 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     1801 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     1861 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     1921 accaacatag catttttaca tcccct
//
LOCUS       DAJTRN010000238         1936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRN010000238 DAJTRN010000000
VERSION     DAJTRN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(64..471)
                     /locus_tag="OO328_004103"
     CDS             complement(64..471)
                     /locus_tag="OO328_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932435.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            548..775
                     /locus_tag="OO328_004104"
     CDS             548..775
                     /locus_tag="OO328_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5932436.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            759..1181
                     /locus_tag="OO328_004105"
     CDS             759..1181
                     /locus_tag="OO328_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932437.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1259..1897
                     /locus_tag="OO328_004106"
     CDS             1259..1897
                     /locus_tag="OO328_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932438.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGDTGTGKIPSAGFYT"
BASE COUNT          524 a          403 c          515 g          494 t
ORIGIN      
        1 gtaaaaaaca caagtgtttg aaagtttgtg ctttttatta cagggtgtgg agaaaaaagg
       61 ggattatttg tttgcgcttc ttttgcgagc tttgagtagt tcttcaaaaa gtttgttgaa
      121 attttcaact cgagcacgca tctctgacaa cagggccttt tgctctgact caggcagtgc
      181 gtcgaacagt tgaagtaact ctttttgatc ttctgtcaga atggctggct gattatccgg
      241 gatcggttcg cctggttgtt tatcttcatc cccaaaaaga agccaagtcg gtgagcactg
      301 aagcgcttgg ctcagtgcga ataatctttt ccccgccggc tgtgtttcat ctctttccca
      361 ttgagaaatt gttacgtgag ccactttgac cagcttacct aatgcggcct gagacagttt
      421 taatttttta cgcctatgta agaggcgagc accgaaggtt tcgtttttca tattagggaa
      481 ttctaatttt tcttgactta ggtttctcta cggcctggtt tccttaggaa aatctaagga
      541 gctcgatatg ttgaaaattg atgctatagc gttttttggc agcaaaacaa agcttgccaa
      601 tgccgcagga gttaggctgg caagcattgc tgcatggggg gaactggttc ctgaaggtcg
      661 cgcgatgcgc ctgcaagagg catctggcgg ggaacttcag tacgacccca aagtttatga
      721 cgaatatcgt aaggcaaaac gacctgggaa ggtgattcat gaaaatcagg catgagcaca
      781 tcgaatcagt gctgttagcc ctggcagccg aaaaagggca ggcgtgggtc gctaacgcaa
      841 ttactgaaga atatctgcgg caggggggcg gcgaattgtc tctggtacca ggcaaggact
      901 ggaacaatca gcagaatatc tatcaccgtt ggttgaaagg tgaaacgaaa gcgcaaaggg
      961 aaaaaattca gaaactgatc cctgcggttc tggcaattct tccgcgtgag ctgcgtcacc
     1021 gactctgtat cttcgatacc ctggaacgcc gtgcattact ggcggcgcag gaagcgttga
     1081 gtacggcaat tgatgcgcat gatgatgctg tccaggccgt ttaccggaaa gcacatttca
     1141 gcggtggtgg gtcgcccggc gattctgtcg tagtgcattg attgaaatta atcgtgccgg
     1201 attgttttgt tcggtatcag ttaaatgtaa cgctgcgagc gttacaaggt gaaaacaaat
     1261 ggcttcaaac tggataaagc tcgaggttat tacgccggat aagccggaaa tattcaggct
     1321 tgctgagatt ctgaatattg atccagatgc cgcattaggg aaggttattc gcttctgggc
     1381 atgggcggat caacaaatga tagacggtaa tgcagattgt aacgctcgcg gcgttacaaa
     1441 aagtgcaata gatcgcatca cttttatgtc tggttttgct gatgcgttaa ttcaggttgg
     1501 atggctggtc gaaaatgacg gtgggctttc tctacctaac tttgaacgtc ataacggaaa
     1561 aagctctaaa aaacgggcgg ttacaaacga gcgagtaaca aaaatacgcg aactgaaacg
     1621 aaaaggtaac gctgccagcg ttacacaaac ggatcaaaaa gcgttaccag tggaagagga
     1681 agaggaagat ctaaatactg atctccccct aaatccccct cgccaaaaac gagcgtctaa
     1741 aaaattcgag ccggaggcta ttgagctgcc cgattggttg ccggaaacac tctggcatga
     1801 gtgggtccgg ttcagacagg cattgcgaaa accgattcga acggagcagg gcgatacggg
     1861 aactggaaaa attccgtcag cagggtttta cacctgagca ggtgattcga cacagcatcg
     1921 ccaatgaata ccaggg
//
LOCUS       DAJTRN010000239         1933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRN010000239 DAJTRN010000000
VERSION     DAJTRN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..371
                     /locus_tag="OO328_004107"
     CDS             <1..371
                     /locus_tag="OO328_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932439.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            371..718
                     /gene="tnpB"
                     /locus_tag="OO328_004108"
     CDS             371..718
                     /gene="tnpB"
                     /locus_tag="OO328_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5932440.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            738..>1933
                     /locus_tag="OO328_004109"
     CDS             738..>1933
                     /locus_tag="OO328_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5932441.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDILRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSEMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHVRRKIHDVHARVPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLM"
BASE COUNT          456 a          535 c          548 g          394 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag ccggataata aaatcccggc tccggcacag gaaccagagc
      241 agataaatac accgtccgac agtctgtgct gtgagctggt tctgccggcc ggaactctca
      301 ggcttaaagg taaactgacg ccggcgttat tacagatact tatccgcgaa ataaaaggga
      361 gcagccactg atgatatctt tccctgcagg ttcgcgtatc tggctggttg caggtatcac
      421 cgatatgcga aatggcttta acggcctggt ctcaaaagtt cagaacgtcc tgaaggatga
      481 cccgttctcc gggcatctgt tcatcttccg cggacgccgg ggtgaccaga taaaagtgtt
      541 gtgggctgac agtgacggac tgtgcctctt taccagacgc ctggagcggg gccgcttcgt
      601 ctggccggtc acccgtgacg gcaaggtgca ccttactccg gcccagttat ccatgcttct
      661 tgagggcatc gactggaaac acccgaaacg aacggaacgc gctggcatcc gcatataacc
      721 cgttgtaaag tgaggatatg gacacctcac ttgctcatga gaatgcccgc ctgcgggcac
      781 tgttgcagac gcaacaggac accatccgcc agatggcgaa atacaaccgc ctgctctcac
      841 agcgggtggc ggcttatgct tccgaaatca accggctgaa ggcgctggtt gcgaaactgc
      901 agcgcatgca gttcggtaaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagatac
      961 tggaagcaca ggagcgaatc agcgcacttc aggaagaaat ggcggaaacg ctgggtgagc
     1021 aatatgaccc ggtactgcca tctcccctgc gccagtcttc agcccgtaaa ccgttaccag
     1081 cctcacttcc ccgtgaaacc cgggtcatcc gtccggaaga ggaatgctgc ccggcctgtg
     1141 gtggcgaact cagttctctg ggatgtgatg tgtcagagca gctggagctt atcagcagcg
     1201 cctttaaggt tatcgaaaca caacgtccga aactggcctg ttgccggtgt gaccatatcg
     1261 tgcaggcacc ggtgccttca aaacccattg cacgcagtta tgccggagcg gggcttctgg
     1321 cccatgttgt tgctggaaaa tatgcagact atctgccgtt ataccgccag tcagaaatat
     1381 accgtcgtca gggcgtggag ctgagccgtg ccacactggg gcgctggaca ggtgctgttg
     1441 ctgaactgct ggaaccgctg tatgacatcc tgcgccagta tgtgctgatg cccggtaaag
     1501 tccatgccga tgatatcccc gttccggtcc aggagccggg cagcggtaaa acaaggactg
     1561 cccggctgtg ggtctacgtt cgtgatgacc gtaacgccgg ttcggaaatg cctccggcgg
     1621 tctggttcgc gtactcaccg gaccggaaag gtatccatcc acagaatcat ctggccggtt
     1681 acagcggagt gcttcaggcc gatgcttacg gtggttaccg ggcgttgtac gaatccggca
     1741 gaataacgga agccgcatgt atggctcatg tccggagaaa aatccacgat gtgcatgcaa
     1801 gagtgccaac cgacatcacc acggaagccc tgcagcgtat cggtgaactg tatgccatcg
     1861 aggcagaagt ccggggatgt tcagcagaac agcgtctggc ggcaagaaaa gccagagccg
     1921 cgccactgat gca
//
LOCUS       DAJTRN010000240         1909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRN010000240 DAJTRN010000000
VERSION     DAJTRN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..170)
                     /locus_tag="OO328_004110"
     CDS             complement(<1..170)
                     /locus_tag="OO328_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932442.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..>1909
                     /gene="mutS"
                     /locus_tag="OO328_004111"
     CDS             246..>1909
                     /gene="mutS"
                     /locus_tag="OO328_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5932443.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVL"
BASE COUNT          454 a          522 c          536 g          397 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgct
//
LOCUS       DAJTRN010000241         1902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTRN010000241 DAJTRN010000000
VERSION     DAJTRN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..128
                     /locus_tag="OO328_004112"
     CDS             <1..128
                     /locus_tag="OO328_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5932444.1"
                     /translation="RRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            477..1649
                     /locus_tag="OO328_004113"
     CDS             477..1649
                     /locus_tag="OO328_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5932445.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
BASE COUNT          619 a          363 c          331 g          589 t
ORIGIN      
        1 ttcgtcgata tgctcacctt gcacctaatc accttaccga acacgcacgg caaatagact
       61 cgatcctgaa cccatcggtc ccaaattcgt cccagtcaaa aaataaggaa ggtactaatg
      121 atgtgtaact tattgattta aatggtgccg ataataggag tcgaacctac gaccttcgca
      181 ttatgaatct gtagcaccaa tcataactat ctgttttagc aagcattaac cgcattcact
      241 aagctatagt tgatgacaca aacagaaagt tgatgcatga tgttgccata tatatgtcac
      301 aaatacggca caacgatctt caaacatata gccacacatt cacagaagag cacaaagcct
      361 tgcaatccag tgcaaagctt ggtgtgtctc cgttttgttt catcagttac acataactat
      421 atgatcatta actaaaagtc agtatacttg gagaaatttt aattgcgtaa tagagcatgc
      481 atatgattga aattaacagt ttattattaa taacatccgt gatattgatg tcgttattag
      541 ctgtggggtt atttgataaa atttccccaa taaatcttgt tgagcacggc aggaataatc
      601 aaatagatgg aatgcggggg ttcttagcaa ttttcgtgct tattcatcac gcagcaattt
      661 ggaatggata cttgtcatct ggagtatggg aagcaccttc atcaaatctg ttagcaaatt
      721 taggccaagt tggtgtgtca ttctttttta tgattactgg ttatctgttc ttttcaaaga
      781 ttatctctgg agatcaggac tggacaaggc tttatgtatc aagattacta cgattaaccc
      841 caatgttcat agttagccta tgtctcattt ttatcattgt aggttttaag tctggatgga
      901 gaatgcaggt atccacagaa gagctttttg tgtcaataat gaagtggttg ccattcactg
      961 cgctaggtat gccgaacatt aatgacgtaa aagattcatt tactatcaat gccgctgtaa
     1021 catggacact tgtatatgaa tggttctttt atttttctct tccggtaatt tccgcgctca
     1081 taaaaagaaa agtcagtatt tacatggtta tgattagcgc aatatcgcta tttgttttca
     1141 ttttattttt cagtaaaata cacatagcat catttctttt tgggctcctg gcatttttgc
     1201 taaataaatc aaagatagtc aatggaattg ccaaagcaaa agtaacacca attataataa
     1261 ctgcgataat ggtttttgaa atgacgtatt tcaaaacaac ttacgcaccg ctaccactga
     1321 ttctttgtgg aataacattt atcattatcg catcaggttg tgatttgtat ggaatattaa
     1381 gattaaatat aaccagaaag ctaggagaaa ctacctacag cgtttacctt ctgcatggga
     1441 tattccttta ttgcctaatg acgtggatta ttcctaataa ttacactgaa aatacattta
     1501 tcatactggt aagcacaact gcatttctta ttacgttcac atcatgccta acttttaaat
     1561 taatagagac gccattcatc aaattaacaa aacaaaccac aactttagta aaagaattaa
     1621 tacccacatt aacaaacaac aatcaataaa caataaacat aagccgccct acatatatgt
     1681 ccaaatgggg cggcgaatgt atttatatta gtctggtaat gctaaatgca aacgaactgg
     1741 agattcctgc cccgctaagc gtattgcgca gggtcaccct aaatgtatct acgccaatat
     1801 aagtaacatc acaactaact gaatcaggcg cagatatagg tgttatctgc acaatataac
     1861 cgctagcaac tgctgcgagc ggatgagaaa agctaacatc at
//
LOCUS       DAJTRN010000242         1894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTRN010000242 DAJTRN010000000
VERSION     DAJTRN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(43..1692)
                     /locus_tag="OO328_004114"
     CDS             complement(43..1692)
                     /locus_tag="OO328_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5932446.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          445 a          502 c          485 g          462 t
ORIGIN      
        1 attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt cgccgtacaa
       61 accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca acggatactc
      121 gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga tcattttgtg
      181 ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt catcacccag
      241 aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg gtgcccagcc
      301 gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc cgccaggtgt
      361 cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt tcgccatgcc
      421 gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc gtcgccagtc
      481 gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg catcgcgacg
      541 ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca gcgccgagat
      601 gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg ggatgaactg
      661 ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag agtaatccca
      721 accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc gaccagaatg
      781 tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc gatcgtccca
      841 gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg cctgattagg
      901 gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt ggtcgagata
      961 gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg caaaaaccgg
     1021 tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac cgtagttttc
     1081 gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac cactttccgc
     1141 cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac ggccgcccgg
     1201 gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat cctgtggttc
     1261 gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt gcgggtccga
     1321 tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa acttgcgcaa
     1381 gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca gcgggtccat
     1441 tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg actgaacatg
     1501 ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg gcagcccttc
     1561 aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt catacgggtc
     1621 cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt tttgaatttt
     1681 ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg cttttcttta
     1741 agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca cgtttattaa
     1801 cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg gatttggtgt
     1861 tgtttttcat tttcagaaca ttcaatcctg ctct
//
LOCUS       DAJTRN010000243         1851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTRN010000243 DAJTRN010000000
VERSION     DAJTRN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..249)
                     /locus_tag="OO328_004115"
     CDS             complement(<1..249)
                     /locus_tag="OO328_004115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932447.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI"
     gene            complement(1002..1475)
                     /locus_tag="OO328_004116"
                     /pseudo
     CDS             complement(1002..1475)
                     /locus_tag="OO328_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1403..1769)
                     /locus_tag="OO328_004117"
                     /pseudo
     CDS             complement(1403..1769)
                     /locus_tag="OO328_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          516 c          437 g          473 t
ORIGIN      
        1 aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt ttttgtttcc
       61 ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt
      121 atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt gaagttttac
      181 gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg gtttagctga
      241 acgggccata taactacctg aaagacaatg atattgtctg tttttataac ggtaatcaca
      301 ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa cgcttattta
      361 gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat tgtccttatt
      421 ccactaatac aaactttttg taaagtatat taaatatact tcatttcata ctgaaatccc
      481 cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca attaagttgt
      541 caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca ttggtactct
      601 ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca ggtccacgtt
      661 ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc gcaggcagta
      721 aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg ctttcgacac
      781 atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc tcacgtccga
      841 ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg atggtttggt
      901 gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct gcatggctgc
      961 acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc atagcaacag
     1021 taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg gcctcgctgg
     1081 atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta gcgtttattc
     1141 agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat cacaccgccc
     1201 tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg ttttccacag
     1261 cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca gcagccggtg
     1321 atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc ccgtgtttcc
     1381 ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg ttcgggccac
     1441 tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt gcacggtaac
     1501 tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc agactggctg
     1561 ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca ggcgtggggc
     1621 accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg ctgacggaac
     1681 tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc gctgcgggcc
     1741 acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg cggcaacggc
     1801 tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa g
//
LOCUS       DAJTRN010000244         1838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTRN010000244 DAJTRN010000000
VERSION     DAJTRN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..301
                     /locus_tag="OO328_004118"
                     /pseudo
     CDS             1..301
                     /locus_tag="OO328_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            470..1834
                     /locus_tag="OO328_004119"
     CDS             470..1834
                     /locus_tag="OO328_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5932448.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          478 a          351 c          414 g          595 t
ORIGIN      
        1 gagacgtaca gtggttttgt ccgctcacac cagtacatac aggtacggaa ataaaaccag
       61 tcgaaatgcc ggtaacgacg attactcctg tctctgatgc cggtggacta caagatttta
      121 tttactggcg tcctgatgcg gacagggctg gtatggaacc tgtttatgtg atacttaatg
      181 atcctctgga ttccgggcga tttacgcgta aacaactaaa taaaaatatc ttaaacatgc
      241 aagtaatttt ggtattctcg atacaagaat aaatagtagt aactactata gctgcaaata
      301 atgttaaaaa catttctatg atcatcttta catttttcat gattgacaat tgtttttggt
      361 taaaaaaaga caactaatga gtgtgataat gcaatacttt gagtgattat ataatgataa
      421 gtaaacaggt ttgcgacaat tggttatcag tattacagaa ggattaaata tgtcattatt
      481 cacagaacct aaggaaattg aaagattacc ctctgaagag attgaacgat tatatcccgt
      541 cctgcgatac agggtcttta tcagcatttt cctcggatat atggggtatt attttgtccg
      601 aaacaccacc agtgtgttat ccggtgtgct gcacatgtct gcaaccgaga tcggcattat
      661 ctcctgtgcc ggttttttat cctatggtat atcaaagttt gtttccggac ttatttctga
      721 cagaagtaat tcaaaagtat ttttgtctct tggtttattc ctgtccggac tggtaaattt
      781 tctgatagga tatattcccg ggatcatcac atccgttacg ttattttcaa caatgtattt
      841 acttaacggc tggatccaag gtatgggata tcctcctggt gccaaaacac tggtgttctg
      901 gtatgaacat cgggaaagga tcacctgggc cacactgtgg aatttatcgc acaatgtcgg
      961 gggagctctg gcccccgttc tgattggttt cagttttggt tttttcggcg acagtgctct
     1021 ggatcatgcc cgggccgcat ttatcttccc aggagtactg tgtatggcaa tgagtgtgct
     1081 gatttacttt attcaggtgg acagacctgt atcagtgggg cttcctccca ttgaagaatg
     1141 gaaaggtaac gtcgtttcac acccggctaa aggccgtgaa caaggtccga gactctccat
     1201 tcctgatatc atcagaaaac atatcatccg aaataataaa ctcatttact gctgtatcta
     1261 tggttcgttt gtttatattc tgagatacgg tattgtttcc tgggctccta agtttttaag
     1321 tgacagtctg gatgtcggtg gtaaggatat gggaaaactg gcatcaatgg gggggggttc
     1381 tgtttttgaa atcggtggtg tggctggtat gcttcttgcc ggatacctgt ctgtcagact
     1441 gttcagaaac tcaaaaccat taacaaatac tttatttctg gcacttacca ttattttatt
     1501 aatcgcgtac tggtacgtgc catcaggcaa tgaatatctc tggctaaact atacaatcct
     1561 tattctgttg gggctggcag tgtatggtcc ggtgatgttt attggtcttt attcaatgga
     1621 gcttgttcct aaagaagctg ccggagccgc atcagggctg tcaggcacat tttcctatat
     1681 cttcggctca attgttgcga cactgggaat gggacttgtt gtcgattatc tgggatgggg
     1741 ggccacattt atcgtactga tactgtctgc tgtttttgcc atcattttca cactgatgag
     1801 tcgtgaacgc tcactggagt tcgaaaaaga gtaatttt
//
LOCUS       DAJTRN010000245         1817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTRN010000245 DAJTRN010000000
VERSION     DAJTRN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="OO328_004120"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="OO328_004120"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5932449.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..>1817
                     /locus_tag="OO328_004121"
     CDS             1284..>1817
                     /locus_tag="OO328_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5932450.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDN"
BASE COUNT          416 a          469 c          535 g          397 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataat
//
LOCUS       DAJTRN010000246         1816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRN010000246 DAJTRN010000000
VERSION     DAJTRN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(198..599)
                     /locus_tag="OO328_004122"
                     /pseudo
     CDS             complement(198..599)
                     /locus_tag="OO328_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1028..1816
                     /gene="virF"
                     /locus_tag="OO328_004123"
     CDS             1028..1816
                     /gene="virF"
                     /locus_tag="OO328_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5932451.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          566 a          329 c          336 g          585 t
ORIGIN      
        1 tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac
       61 attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga
      121 gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt
      181 cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag
      241 agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg
      301 ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc
      361 atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc
      421 agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg
      481 gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat gacatcctgc
      541 accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa
      601 tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc
      661 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
      721 ataggatatt atgatgctgg agttttgcaa agctccactc ctttccgaac atttacacag
      781 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      841 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      901 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      961 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
     1021 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
     1081 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
     1141 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
     1201 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
     1261 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
     1321 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
     1381 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
     1441 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
     1501 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
     1561 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
     1621 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1681 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1741 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1801 tcataaaaaa ttttaa
//
LOCUS       DAJTRN010000247         1810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRN010000247 DAJTRN010000000
VERSION     DAJTRN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..234
                     /locus_tag="OO328_004124"
                     /pseudo
     CDS             1..234
                     /locus_tag="OO328_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OO328_004125"
     CDS             655..1233
                     /locus_tag="OO328_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932452.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OO328_004126"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OO328_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..1810
                     /locus_tag="OO328_004127"
                     /pseudo
     CDS             1584..1810
                     /locus_tag="OO328_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          588 a          322 c          423 g          477 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggaaggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc
//
LOCUS       DAJTRN010000248         1785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRN010000248 DAJTRN010000000
VERSION     DAJTRN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..325)
                     /gene="flhe"
                     /locus_tag="OO328_004128"
     CDS             complement(<1..325)
                     /gene="flhe"
                     /locus_tag="OO328_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5932453.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEV"
     gene            complement(325..1785)
                     /locus_tag="OO328_004129"
                     /pseudo
     CDS             complement(325..1785)
                     /locus_tag="OO328_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
BASE COUNT          413 a          553 c          442 g          377 t
ORIGIN      
        1 gcacttccca gataaatcgc aacggttctg ctgccgctat accggaaaag gccacggtgg
       61 taccgctctg cccctctaat tcgacacaac gagattgcga acacaagcga acccgcagtc
      121 ctgcaggtgt cgggccgata agctgataac gccacgccac cagcgtcatc aatcctgaag
      181 caggttgtcg cgcagaaaga ggtgcagacg acatcgactc accgcgatga tttagcgtaa
      241 ttccgacact acttgcctgc cacatcccct ccccggcggc ttgcaccagc agcggaaaca
      301 ataatatcgc taataaggtt ctcatcattt accgccaatt gtcgccgtca tgcggatatg
      361 tcggttatca gacagttcca gattcgacag gaccactaac tgcggcaagc tgcggcgcag
      421 gaagcgagac aataatggtc gcagcgcgtg gttcaccaac aatactggcg gcgcacccag
      481 catctcctga cgggatagag cttcctgagt ttgcgccagt aaacgatccg ccagccctgg
      541 ctccagtcct cccccgccct gcagcgcctg tagtaacaaa cgttccagcg gtgtatcgag
      601 gccaataaca tggacttcat ctttgccagg aaaccactgc tgggtaatcg cccgtcccaa
      661 cgccacgcgc acgacggcgc ttaattcatg tggatcgctt tggatgggcg catgttccgc
      721 cagcgtttcg agaatggtgc gcatatcgcg aatcggtact ttttcatcga ggagattttg
      781 cagcacttta tgcagtgtgg tgagcgtgac gacgccagga acgagatctt ccgtcagctt
      841 tggcatctcc tgggcgacgc gatccaacag ctgttgcgcc tcctgacgac caaacagctc
      901 tgcggcatgc tggctaatga ggtggttaag atgcgttgct accaccgtgc tggcctcaac
      961 cactgtgtac ccctgaatct gcgcctgttc ttttagcgca ctttcaatcc agatagcatt
     1021 caggccaaat gccggatcga cggtcgcctc accaggtaac gtcccggcag cggttccagg
     1081 gttaatcgcc agccagcgcc ccggataagc atcaccactg ccaatctcca cgcctttcat
     1141 caaaatgcga tagcgggcag gttgcagatc catattgtcg cgaatgtgca ccactggcgg
     1201 cagaaatccc atctcctggg caaatttctt gcggatactg cgtatacggc ccaacaactc
     1261 accatcctgc tggaaatcga ccatcgggat cagtcgataa cccacttcca ttcccagaga
     1321 atcttccagt tgtacatcgt tccacgtcgc ttcgacaacg gtattattct ctgccatttt
     1381 taccggtttg ggttcggcag gcgctttttg ttcgcgtccg cgtatccacc aggccagccc
     1441 cagcaatccg gcagtgaaca gcaaaaatac caggttcggc attccaggca ccaggccgag
     1501 taaaccgagc acggcggcgc ttaacaacat aacgcttggg ttactgaaaa gctgattcac
     1561 catctgctcg ccaacatcct gatcggtgct gacacgcgta acgatgcccc ccgcggcggt
     1621 agaaatcacc agcgccggaa tttgtgccac cagaccgtcg ccaatggtca atagcgtata
     1681 actttccgcc gcgtgtccca tgctcatgcc agttgcagca cgccgaccag caacccgccg
     1741 acaacgttaa tgaccatgat gaggatcccg gcaatggcat cgccg
//
LOCUS       DAJTRN010000249         1753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTRN010000249 DAJTRN010000000
VERSION     DAJTRN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO328_004130"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO328_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5932454.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO328_004131"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO328_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1753)
                     /locus_tag="OO328_004132"
                     /pseudo
     CDS             complement(1292..1753)
                     /locus_tag="OO328_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          516 a          310 c          396 g          531 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tat
//
LOCUS       DAJTRN010000250         1716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTRN010000250 DAJTRN010000000
VERSION     DAJTRN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..326
                     /locus_tag="OO328_004133"
     CDS             <1..326
                     /locus_tag="OO328_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5932455.1"
                     /translation="LKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKPVLSAL
                     KAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDTALETFWQALHRRDVQVPDLLSLR
                     GNAHA"
     gene            319..1278
                     /gene="ssuA"
                     /locus_tag="OO328_004134"
     CDS             319..1278
                     /gene="ssuA"
                     /locus_tag="OO328_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5932456.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1275..>1716
                     /locus_tag="OO328_004135"
     CDS             1275..>1716
                     /locus_tag="OO328_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5932457.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGET"
BASE COUNT          388 a          478 c          459 g          391 t
ORIGIN      
        1 cgctgaaaac cctgctcgac ctgctgccag agcgcgcttt gcaaggcaaa gtggtgctac
       61 cgctggcgac gggcggtacc gtggcccatc tgctggcggt cgattatgcc cttaaaccag
      121 ttttaagcgc actgaaagct caggagatcc tgcacggcgt gtttgccgat gactcacaat
      181 taattgatta ccatcacaga ccccagttca cgccaaatct gcaaacccat cttgataccg
      241 cgctagaaac tttctggcag gcattgcacc gccgcgatgt tcaggttcct gaccttctgt
      301 ctctgcgagg taatgcccat gcgtaacatc attaaactgg cgctggcggg attgcttagc
      361 gtctctactt ttgcggttgc tgcagaatcc tcgcctgaag cgttacgtat aggctatcag
      421 aaaggcagta ttggtatggt gctggcaaaa agccaccagt tactggaaaa acgctatccg
      481 caatcaaaaa tctcctgggt ggagttcccc gcgggtccgc aaatgttgga agcgttaaac
      541 gttggcagta ttgatctcgg cagtaccggg gatattccgc caatctttgc ccaggctgcc
      601 ggggctgatt tggtatatgt gggcgtcgag ccaccgaagc ccaaagccga agtgattctg
      661 gtggcagaaa acagcccgat caaaaccgta gccgatctta aaggtcacaa agttgccttt
      721 cagaaaggtt ccagttcaca caacctttta ctgcgtgcac tgcgtcaggc cggacttaaa
      781 ttcaccgata tccagcccac ttacctgacg cctgccgatg cccgcgccgc gttccagcaa
      841 ggtaacgttg acgcctgggc tatctgggat ccctactact ccgctgcgtt attacagggc
      901 ggcgtgcggg tgctgaaaga cggcaccgat ctcaatcaaa ctggatcgtt ttatctggca
      961 gctcgtccct atgcagaaaa aaacggcgct tttattcagg gcgtactggc aacctttagt
     1021 gaggccgatg cgttaacccg cagccagcgc gaacaaagca tcgctttact ggcaaaaacg
     1081 atgggcttac cggcaccggt gattgcctct tacttagatc atcgccctcc taccaccatc
     1141 aaaccggtta acgccgaggt tgccgcctta cagcagcaaa cggcagatct gttttatgaa
     1201 aaccgtctgg tgccgaaaaa agtcgatatt cgccagcgca tctggcagcc cactcaactg
     1261 gaaggaaaac aattatgagt ctgaatatgt tctggttttt accgacccac ggtgacgggc
     1321 actatctggg aacggaagaa ggttcacgcc cggttgatca cggttatctg caacaaattg
     1381 cgcaagcggc ggatcgtctg ggctataccg gtgtgctgat cccaacgggg cgatcgtgtg
     1441 aagatgcgtg gctggttgca gcgtcgatga tcccggtgac gcagcggctg aagtttcttg
     1501 tcgccctgcg tcccagcgta acctcaccta ctgttgccgc ccgccaggcc gccacgcttg
     1561 accgtctctc aaatggtcgt gcgttgttta atctggtcac aggcagcgat ccacaagagc
     1621 tggcaggcga cggcgtgttc cttgatcata gcgagcgcta cgaagcctcg gcggaattta
     1681 cccaggtctg gcggcgctta ttgctggggg aaaccg
//
LOCUS       DAJTRN010000251         1708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTRN010000251 DAJTRN010000000
VERSION     DAJTRN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..541)
                     /locus_tag="OO328_004136"
     CDS             complement(<1..541)
                     /locus_tag="OO328_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR5932458.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKI"
     gene            complement(492..1256)
                     /locus_tag="OO328_004137"
     CDS             complement(492..1256)
                     /locus_tag="OO328_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932459.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1451..>1708
                     /locus_tag="OO328_004138"
     CDS             1451..>1708
                     /locus_tag="OO328_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5932460.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGH"
BASE COUNT          415 a          429 c          380 g          484 t
ORIGIN      
        1 taattttgcg gatgagagtg ccttcgggcg gatcaacaac gaagttgcgc cagacgaaat
       61 cagtaacaag tccggggttg aatatgaacc agcattccga accctcttta cggatcgttg
      121 gctccagaat tttccactgg tattccgtca gtgcgtgggc ttcttccaac cacaacatat
      181 cgatcccctc cagtgactta atttcttcga tgttgcgcca taatccataa aaaacaaatt
      241 ccgagccagt aacccggtta atgattttgt tgttcagaat acggaaacgg tgccgcagac
      301 caaacctgtc tatctgaatt ttgagcaggg tatacaccga ctcttcgatt ttattctgga
      361 tctgacgggc acaacaaaaa cgcagggtgt atttattcga cagaaatatg gcaatgccag
      421 cggcatccca tgattttgac gatgaccgac caccataaag cactttgtta cgtgcccgca
      481 tagtccagaa actacgtaaa gccggattca gcgtcggttt ggatgtcaga gtagaagtca
      541 ttgaggtcac gctctccatt gccatcatca atacctgcat cacggcgaag acgatcagcc
      601 tccagagaca ccttatcagt ggcggccttg cggtaggctg tatcagcaaa tatttttcct
      661 accgtcgcaa gcgtgccaac gatggactca atacgaactg tattgcgcat cattgctttc
      721 tcggcggcgc tgatattttc aatcagcatc ttcctttcct ggtccccatt agcatcttcc
      781 agcgacacca accaccgacc aatattctct gcagcgacaa ggttgttagc acgaaggcga
      841 aataattcgt cctcgagcgt caacgcttta gcgtcctcta tcacctcatc tttgagcaga
      901 aggcgacggg cataaccacc gtgttttaat gcctgctggt taccgggttg gaatgggtta
      961 gtcgggggat cggtacgcat tccgcgtatc ggtttcgtat ccggtgtaga ttctgttttt
     1021 agttgcgtac ttttttgtgt aaggccagta atggcaggct ttctgctggt acgcactttc
     1081 cttttttgcg taccattttt gcaaacctgc gtaccgccac tgcgtgccca atcaagcttt
     1141 ttggccctct tcctgatagc cccttctgta acgccgtatt tctcgcctat atcacggagg
     1201 ctaaggactc cggcccggta tgccgattcg atggcctccc agtccggttt tgccatgatt
     1261 atgcttcctg tgatttactg ttatcgctgc ctgtcacaat aggttgcttt aattcttcct
     1321 catcactcac tcctgcgacg ctccgtggca gggcccccca caggagtacc ttgtaaatgt
     1381 aaggtgttta agcctaaatt aagtcgtatc acggttaaat agcaaagaaa aattcacaga
     1441 aggaaagaca ttgatggagt ggataaatgc tttgatgaac tattttgtac ttcatccata
     1501 tcgcctatgg gggcttcttt ttataatagc gttcacaaaa tccactttac tcatttcggc
     1561 gatattgcca ccagcatcaa ttatgatggc cactgtaatt acagtcagca agacgactct
     1621 ttcaccatgg gaagctggaa tcacggtcat gagtggcgca tggtgcggta ctatcgttaa
     1681 ttaccatctt ggtataataa ttgggcat
//
LOCUS       DAJTRN010000252         1670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRN010000252 DAJTRN010000000
VERSION     DAJTRN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            230..499
                     /locus_tag="OO328_004139"
                     /pseudo
     CDS             230..499
                     /locus_tag="OO328_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            496..722
                     /locus_tag="OO328_004140"
                     /pseudo
     CDS             496..722
                     /locus_tag="OO328_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            751..1341
                     /gene="ospG"
                     /locus_tag="OO328_004141"
     CDS             751..1341
                     /gene="ospG"
                     /locus_tag="OO328_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5932461.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          493 a          347 c          388 g          442 t
ORIGIN      
        1 ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag tgataggaca
       61 ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta ctcctctgcc
      121 atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct ggtgacgata
      181 tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt
      241 taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg ctgcaaaaag
      301 catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg ttgaacggca
      361 gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg aatgcggtga
      421 tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg aaatgagcag
      481 gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca ggacaggaag
      541 cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg gacgaacgga
      601 ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg
      661 aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt
      721 gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat tattcaaaca
      781 ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc cattctcggt
      841 aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga ttcatctgct
      901 ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga aatggcttgg
      961 caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt tatacagtat
     1021 ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag tgacattgat
     1081 acagctgata tccctgataa tatagagagc ctttatctac agttgatatg taaattgaat
     1141 gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga taaagaaagt
     1201 gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc tgcaaccaaa
     1261 aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt ttattcgtta
     1321 ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg ctcttctatg
     1381 agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta tgcaccgcca
     1441 gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa
     1501 aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca acaaggcgaa
     1561 tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt aaaccgtctg
     1621 gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
//
LOCUS       DAJTRN010000253         1665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRN010000253 DAJTRN010000000
VERSION     DAJTRN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            3..749
                     /locus_tag="OO328_004142"
                     /pseudo
     CDS             3..749
                     /locus_tag="OO328_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            764..1186
                     /locus_tag="OO328_004143"
     CDS             764..1186
                     /locus_tag="OO328_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5932462.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1244..1600
                     /locus_tag="OO328_004144"
     CDS             1244..1600
                     /locus_tag="OO328_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932463.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          458 a          358 c          480 g          369 t
ORIGIN      
        1 ccatgaaaaa tattgcgaca ggcggcgttc tggaacgcat ccgccgactg accccgcaac
       61 atgtaaccgc cccattcaga acggtagcgg agtggcgcga gtggcaactt gctgaaggcc
      121 agaaacgtag cgaggagatc aaccgcctga atcgccagtt gcgggtggaa aaaattctga
      181 atcgctcagg catccagccg ttgcaccgta aatgctcgtt tgcgaattac caggtgcaga
      241 acgacggcca gcgatacgcg ttaagccagg cgaaatccat cgccgatgaa ctgatgaccg
      301 ggtgtacaaa ttttgcgttc agcggaaaac ctgataccgg gtagaatcac ttagcggcag
      361 ctatcgggaa tcgcctgctg aaagacggtc agacagtgat tgtggttacc gtggctgatg
      421 ttatgagcgc cctgcacgcc agctatgacg acgggcagtc aggcgaaaaa tttttgcggg
      481 agctgtgcga agtggatctg ctggttcttg atgaaattgg cattcagcgc gagacgaaaa
      541 acgagcaggt ggtgctgcac cagattgttg atcgccggac agcgtcgatg cgcagcgtgg
      601 ggatgctgac aaacctgaac tatgaggtga tgaaaacatt gctcggcgag cgggtgatgg
      661 atcgcatggt catgaacggc gggcgctggg ttaattttaa ctgggagagc tggcgtccga
      721 atgttagcca ttcgagggtt gttaagtagt ttcaggagga tttatggcga aaccttttac
      781 tcccgaacag cgggaagaac tgaagacgcg aattgtggaa ctcgtgcatc aggacggtcg
      841 ggtcacgatt cggcagttgt ccgatgaaac aggtatcagt cgtgcgtctg tcggtcgctt
      901 atgcatagaa ctggtcgcaa gtggtgatgt atataattct ggctacggct tattcccgtc
      961 tgaacaggct cgcaaggact ggcaaagcgc ccgcaaaaaa ctctcgagag taaaggtgag
     1021 gaaaccggtt gttgttgatc cggaccttat ctggtcatta cctgacggag aaatacgccg
     1081 ctacgacagg cgcctgaata taatctgtcg cgagtgccgg aagagcgaag ctatgcagcg
     1141 tgtactggct ttctatcagg gtaattttca ggaggcgata ctgtgaatga aattagctat
     1201 caggcttcaa ttaccgctgg cattcgcatc aaaggagagg agcatggaaa taaaaccaga
     1261 agatgagtta agcaatatcg ttttatttcc gataaaagag gatgaccctc gtaatcaggt
     1321 taattttctt tataagccat cggaaagacc atattgccat cacgcctctg tacgggttga
     1381 cgaaaaagag cgtcaggtcc gctgtaaaat ctgcggtgcg gttgtggagc cgtttgactg
     1441 gatgctctct gtggcaaaaa gagaaaccag actggcagat gatgtaaggc tattgcgcca
     1501 ggaggaacag gaaaggcgga gaaatataga aaagctgata cagattgagc gtaacgcgaa
     1561 agcgcggata cgcagggcga caaaatccag aactgaataa ttaaatttag cactgttaaa
     1621 aatttaatcc ttaaccggag ggatttctgc accctcaaat catca
//
LOCUS       DAJTRN010000254         1636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTRN010000254 DAJTRN010000000
VERSION     DAJTRN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(428..793)
                     /locus_tag="OO328_004145"
     CDS             complement(428..793)
                     /locus_tag="OO328_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5932464.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            complement(793..1458)
                     /locus_tag="OO328_004146"
     CDS             complement(793..1458)
                     /locus_tag="OO328_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932465.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            complement(1455..>1636)
                     /locus_tag="OO328_004147"
     CDS             complement(1455..>1636)
                     /locus_tag="OO328_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5932466.1"
                     /translation="CNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVEMKSD
                     PVVFWFEIYQEGATA"
BASE COUNT          415 a          452 c          344 g          425 t
ORIGIN      
        1 gtaagaggct aacctacatt tgtgagacat agattgggcc tcagattaat gttaagcgtc
       61 ttgcaggacg cgtaatgtta actggggctt ttctctgtct gccttacagt ggcatgcccg
      121 aggcagacag cctcaagcac ccgcagcaat cttaccgata ccgataagaa aacgctattt
      181 tttattgcca gcaccttcta tccaggctaa tttatctgcg tcagaacggc gcaatgcgct
      241 cgcctgagat acgtttactt gccatggtaa caacaggtaa cggcaattgc tgtttctgtt
      301 ttgtttcctt caaaaacccc ggaccatcaa tccggggttt ttgtttgtta accccaacgg
      361 caaatcgaat acaccaccag cgccaccgcc atcgcaattc ctaccgtggt gaatgcctca
      421 ggccaggtca ttgattcacc tcctgcggtg gttccggtag cggcatccag tgagttgagc
      481 tctctacttc aacgccgtct ttgtccacaa aagccatctt attcccgcca cgagtggggc
      541 aaaaaacctt atcccaggaa ccggggaata cattcccgag atcatcgaga agaatcacat
      601 cgcaatattc agcaggaact gcatcactac agcttatcca gccagccaga gttaccggag
      661 agttgccagc cttacgcatg gccatttcca cgatttcaac catatctcct ggtggaattt
      721 tacaaagttg cccaatacat ttctgctgcc tggcatattc gagaatgtgc tccagtttgg
      781 ttcgattaat cattatttat ctcccttaag catggcagcg cggcaggcgt tccagccatc
      841 aacataatca aacgtattac tatcgtttgg cccgatttca tccggcacta tcggctctgg
      901 ttggatagtg acgttggcaa aggcagcacg caaaccggtt ttaatttctt cgatttcatc
      961 agagccaagc gatgaatctg acagtgcgtg atggaatgcg taagccatgt cgtcgtttac
     1021 tgcaaccggc actgccggcg ctgcgtaaag cggtgttata tctgcccgaa aatcacatgc
     1081 tttatgcagt cgtacccaac gctcaacttc cgctttgtaa aaatatatag cagtgaacgt
     1141 attagattcc tggtcaactt gagtaaacgt caccttccac gctacagcta cggctttcca
     1201 tccatcaggt tgtcgcattc gcggggcagc ataaagctca tgcattccat caggcagcga
     1261 gtgacccaca tactcaccga acccatcaat acacatgccc ccatcttcaa caatgcattc
     1321 cgctacaggc tctgcttcca gtgatgccag agcgataagc gccagttcac gaatttcacc
     1381 gccgtctata tcgtcaatat catcgcggcc agaaatgtta gccagccatt gcagtcgctc
     1441 tttggtaata gtagtcatgc cgtagcccct tcttgatata tttcaaacca gaacacaacc
     1501 gggtcagatt tcatttcaac caatcccata cgaaccagcg ctttgccttt cccggacgca
     1561 aggaattcac gacgaccatc actgataatt cgtcgataat cttccaggct actgcaatgc
     1621 ttgtgcagat tgcatg
//
LOCUS       DAJTRN010000255         1584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTRN010000255 DAJTRN010000000
VERSION     DAJTRN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..1268)
                     /gene="yiaN"
                     /locus_tag="OO328_004148"
     CDS             complement(<1..1268)
                     /gene="yiaN"
                     /locus_tag="OO328_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR5932467.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1271..>1584)
                     /locus_tag="OO328_004149"
     CDS             complement(1271..>1584)
                     /locus_tag="OO328_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5932468.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          483 a          401 c          407 g          293 t
ORIGIN      
        1 catttcaaag ggaggatgat gagatcggga ataaaaacaa atacgactaa taatgaatat
       61 aaaaccagga cgtaagggaa aacgcctctg actgcatcat cgaatttgag ttttgccacc
      121 cctgaaataa cgttaagtac attaccgata ggcggtgtaa ttaagccgat tgagcagtta
      181 atgataaaca tgacaccgaa ataaatcgga tcgattcctg cctctttaac taaaggcatt
      241 aataccgggg taagaattaa taccgtcggc gttaaatcca tgaccatgcc gacaatcagg
      301 atagccacca taatgacgat aaacagcaga cgcggtgaat cgaccaacgg ttgcagtaaa
      361 tcagaaacca tcatcggcag ttcagcaatg gtaatcagcc aggcggagac ttgtgccgag
      421 gccaccagaa acataaccac tgaggtggtt ttcgccgcgc caatgagtac atgccaaagt
      481 gtggcgaagg tcatttcacg gtaaataact gtggcgacaa acagcgcata gaaagcagca
      541 acggccccgg cttcggtagg ggtaaacaga ccggagcgaa acccgccaat aatgatgact
      601 ggaagaaaca gcgcccagat accggagaca aaagagtgcc agatttcctg catcgttgct
      661 ttttgctggc ggggtaaatt aaggcgactg gcctgccgcc accaggtaag catcagcgtt
      721 gcgcccatca ttatgccagg ggcaatgccc gccataaaca gcttactgat ggataatccg
      781 ctggaaacac cgaagataat aaacggaatg gaagggggaa taattggcgc gataatgccg
      841 ccagaggcaa tcagccccgc cgcccggttg accgggtagt tggcgctgcg catcatcggc
      901 accagtagag cggcaacggc ggcggtatcc gcaacggcag aaccagaaag gctcgccata
      961 atcatagctg ccagcacgcc gacgtagccc agcccgcctg gtttatgccc caccagtttc
     1021 atcggcaggt caacaatgcg ttttaacaag ccgcccgcat tcatgatttc acccgccagc
     1081 acaaagaacg gaatcgccag gagggagaag ctatcggctc cgttcaccag cgtttgtgcc
     1141 atgatctgga catcaaacat gtccagccag aacattaacg ccgccccgca caacaacagt
     1201 gcccaggcaa taggcaaacc gatagcaata ccacccaaca gacagcccag aaaaatcagc
     1261 acagccatga ttaagctcct tgcggtggcg acgttagaga attgctacgc gtgatgagtt
     1321 gatataaatg acgcagttca aagaatgcga taacgaggct ggtgggcaga caagcggcat
     1381 acatcaggcc gatgggcaaa ccgaggatcg gtgaataatc gctccagtcc tgaattgttt
     1441 ttagcgttgc gccccaggcc agtgcgccac aaataaataa gattaaggaa tgggtaacca
     1501 gagcgactcg tcgttgccat gcgggggaga gtttctccac cagaaaggtg acctgaacgt
     1561 gggcgttatc cataaaagcc acaa
//
LOCUS       DAJTRN010000256         1582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTRN010000256 DAJTRN010000000
VERSION     DAJTRN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..600
                     /locus_tag="OO328_004150"
     CDS             <1..600
                     /locus_tag="OO328_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate oxidoreductase"
                     /protein_id="HCR5932469.1"
                     /translation="KLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQE
                     QAPTLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHD
                     SKFDLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIK
                     AIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(636..839)
                     /gene="ydfZ"
                     /locus_tag="OO328_004151"
     CDS             complement(636..839)
                     /gene="ydfZ"
                     /locus_tag="OO328_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5932470.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1017..>1582)
                     /gene="ydfH"
                     /locus_tag="OO328_004152"
     CDS             complement(1017..>1582)
                     /gene="ydfH"
                     /locus_tag="OO328_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5932471.1"
                     /translation="SVRFNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRN
                     GSFIRQAIECAVARRAASMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLL
                     TQIADCQLAWDTIENLKATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVER
                     AMTQHLQEISESVRQIRQENSDWFSEE"
BASE COUNT          386 a          366 c          419 g          411 t
ORIGIN      
        1 aagttgcgca tgctcaacgg cagtcattca ttcttggcat atctgggtta tcttgctgga
       61 tatcagcaca ttaatgactg tatggaagat gaacattatc gtcatgcggc gtatggcatg
      121 atgttgcagg aacaagcgcc gacgctgaaa gtgcagggcg ttgatttgca agattacgct
      181 aaccgattaa ttgcacgcta tagcaacccg gcgttacgtc atcgaacctg gcagattgcg
      241 atggatggca gccagaaatt gccacagcgg atgttggatt ctgttcgctg gcatctggcg
      301 catgacagca agttcgatct gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt
      361 ggtgttgatg aacagggaaa tccgatagaa atcagtgacc cactgttacc tgttattcag
      421 aaggctgtac aaagtagtgc cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag
      481 gcaatctttg gtgatgattt gccagacaat agcttgttta ctgcaaaagt gacggaagcg
      541 tacttgtctt tattagcgca tggcgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa
      601 aaaaagcaat gccgccatcg gcggcattca cacacttagt tcatgccgag acgaatcagg
      661 tccagaggtg ccagtttctc ttcacaacct tcaacaacta cggtttttcc gcggcggatt
      721 tgctcagcgg tcagaccttc agtatcaatc gacaggatct tgccagtgtg accggtgccg
      781 ctaaccataa cacggctgcc agtggtgatt gcgttacggt tacgatcgta ggtcgtcata
      841 ggtgttttct cctttctgat ttacagtcga agcccacgta acgggcgcaa tataaatact
      901 cctgtcgagt gaaatatttt ttgtttttga tcaagctcac atcttttttg atgcatgcat
      961 gagccgtaag gcggggggaa aagcctctca tcccggatgg gatgagagta aatgaattac
     1021 tcttcgctaa accagtcgct gttttcctgg cggatctggc gcacggattc gctgatttcc
     1081 tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat cacgtttttg cagggcagag
     1141 aaaatatcaa gatgctggcg taacagcatt tctggtggag aaacatggtc gaaactcata
     1201 tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg tatcccacgc cagttgacag
     1261 tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt
     1321 tgcttgcgct caatggcaat gcgttgctgg tgaagatttt gttccagttg gtagcactgg
     1381 ctttcggtaa tcatgctcgc cgcccgacgc gccaccgcgc actcaatggc ctgacggata
     1441 aaactgccgt tgcgcacctg ggccatggaa attttgttga cgtagctgcc acgctgcgga
     1501 cgaatttgaa tcaggccgtt ttccgccagt ttaataaagg cttcacgaac cggctggcgt
     1561 gacacattga aacgaacaga aa
//
LOCUS       DAJTRN010000257         1569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRN010000257 DAJTRN010000000
VERSION     DAJTRN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>1569
                     /locus_tag="OO328_004153"
     CDS             <1..>1569
                     /locus_tag="OO328_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5932472.1"
                     /translation="ENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQS
                     FVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNM
                     QDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSS
                     LNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALS
                     PAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGAK
                     VILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWS
                     AENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQA
                     LKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITF
                     VIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQ
                     WKRYDSRTPVNLATR"
BASE COUNT          460 a          373 c          377 g          359 t
ORIGIN      
        1 tgaaaatcgc gtctggccga caaaagccga aaaccacctg cgctttaata tcattaatga
       61 taaagaaaac cgggtgaacg gcatcgcact ctattatcgc ctgccaatgg tacaagtgaa
      121 cgatgagcaa agctttgtcg aacaagctga atggagcatg ttagttcagc tgttcaatca
      181 acgtctgcag gaacgcatac agtcgggcga gttgaagact atttctggcg gcactgcgcg
      241 cagcgttaaa attgcacccg attatcagtc gctgtttttc cgtgtaaatg cacgagacga
      301 taatatgcag gatgctgcga atgcattaat ggcagagttg gcaaccattg atcagcatgg
      361 tttttctgct gaagaactcg atgatgtcaa atctacccgc ctcacctggc tgaaaaatgc
      421 ggttgatcag caagctgaac gtgatttacg tatgctgacc agtcgcctgg catccagctc
      481 attaaataat acgccgttct tgtcgccgga agagacatat caactttcga aacgtctgtg
      541 gcagcaaatt accgtgcaaa gtctggcgga aaaatggcag cagttaagaa agaaccagga
      601 cgcattttgg gagcaaatgg taaacaatga gcttgccgcc aaaaaagcat tgtctcctgc
      661 ggctatcctg gcgctggaaa aagagtacgc caacaaaaag ctggcggctt acatcttccc
      721 aggcagaaat ttatcgttaa cagtagacgc tgacccacag gcggaaatta gcagcaaaga
      781 aacgctggct gagaatctga catcattaac actttccaat ggtgccaagg ttattctggc
      841 aaaatccgcg ggtgaagagc aaaagctaca aattattgcc gtatcgaata aaggcgattt
      901 aagtttccct gcgcagcaaa aatcacttat cgcgctggca aataaagcag ttagcggaag
      961 cggcgttggc gaactctcct cttccagcct gaaacgctgg agtgcggaaa actcggtaac
     1021 catgagcagt aaagtcagtg gcatgaatac gttgctctcc gttagcgcgc ggactaataa
     1081 ccctgaacct ggtttccagt tgattaacca gcgaatcacc cacagcacga ttaacgacaa
     1141 tatttgggca tcgctacaaa atgctcaaat tcaggcgttg aaaacgctcg accagcgtcc
     1201 agcggagaaa ttcgcccagc tgatgtatga gacgcgctat gctgatgacc gcacgaaatt
     1261 actgcaagaa aatcagattg cacagtttac tgccgcagat gcgctggctg ccgatcgcca
     1321 gttgttttca tctccagcgg atatcacgtt tgtcattgtc ggtaatgtcg cagaagacaa
     1381 actcgtggcg ttaattacgc gttacttagg ctcaatcaaa cactctgatt cgccattagc
     1441 cgcaggtaaa ccattaactc gcgcgacgga caacgcatcg gttactgtaa aagaacaaaa
     1501 tgaacctgtg gcacaggttt cacagtggaa gcgttatgat tcccggacac ctgttaatct
     1561 ggcgacgcg
//
LOCUS       DAJTRN010000258         1567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRN010000258 DAJTRN010000000
VERSION     DAJTRN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..496
                     /locus_tag="OO328_004154"
     CDS             <1..496
                     /locus_tag="OO328_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5932473.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            560..>1567
                     /locus_tag="OO328_004155"
     CDS             560..>1567
                     /locus_tag="OO328_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5932474.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NG"
BASE COUNT          373 a          381 c          493 g          320 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg tggcggcagc
      961 cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg atgcaaccgt
     1021 cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag caaatgcagc
     1081 cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag aacaggccaa
     1141 aaatgacatt gatgctgcgc tgaccggcac tctgaaaatg gctaaccatc tgtcagaaat
     1201 cgcagcagca ggcgaaaagg cacaacagaa gtcccgggat aatctggggc tgaaaagtgc
     1261 ggccacgatg gaagcacaga gcgacattta cgaccggaca aaaggccgtc tggcgatacc
     1321 cggcgcattc ggctttgggt gtgcttttct gcctgaagat gttatccgtt ttgacactaa
     1381 gagtgatttc ctggcctggg taaggaatgc gctgccaggt gaatattccg ttgctggccc
     1441 ctacggcatc atcatacccg acacacggtt tgaaggggtg ctcagcatcc ggtggactga
     1501 tgcacgccct gagacaacag aaccgcggta cagagccaaa tcccttactt tttacggcat
     1561 taacggc
//
LOCUS       DAJTRN010000259         1443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRN010000259 DAJTRN010000000
VERSION     DAJTRN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..945
                     /gene="tauA"
                     /locus_tag="OO328_004156"
     CDS             <1..945
                     /gene="tauA"
                     /locus_tag="OO328_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5932475.1"
                     /translation="NTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESG
                     ASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEAL
                     VVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQ
                     RGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAF
                     AKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAEL
                     TGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            958..>1443
                     /locus_tag="OO328_004157"
     CDS             958..>1443
                     /locus_tag="OO328_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5932476.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AF"
BASE COUNT          347 a          371 c          443 g          282 t
ORIGIN      
        1 aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc
       61 gtggcgtatc aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct
      121 aaagagagcg gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg
      181 gcgctggctt ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca
      241 gccagccaac aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa
      301 gcgctggtgg taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc
      361 gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt
      421 aaaccagggc tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag
      481 cggggtgata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac
      541 ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc
      601 tgggtggtgc gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa
      661 agcgccatcg atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg
      721 gaaaacatca gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg
      781 gtgaagggaa atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg
      841 aacaaagcaa tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta
      901 gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg
      961 ctgcaaatct ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac
     1021 ctgacgctgg aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc
     1081 accctgctga atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg
     1141 ggtaagcgta ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg
     1201 ctaccgtggc gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag
     1261 aaaatgcagc gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca
     1321 gaaaaacgct atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc
     1381 gcgctggcgg cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc
     1441 ttc
//
LOCUS       DAJTRN010000260         1442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTRN010000260 DAJTRN010000000
VERSION     DAJTRN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..304)
                     /locus_tag="OO328_004158"
     CDS             complement(<1..304)
                     /locus_tag="OO328_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5932477.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEH"
     gene            complement(370..609)
                     /locus_tag="OO328_004159"
     CDS             complement(370..609)
                     /locus_tag="OO328_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5932478.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(744..1349)
                     /locus_tag="OO328_004160"
     CDS             complement(744..1349)
                     /locus_tag="OO328_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5932479.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          442 a          324 c          250 g          426 t
ORIGIN      
        1 catgttcatc aatagctaac gaaagaacag ttaacagctt gacatcaccg ccgccaatta
       61 tccctgcgga aaaaagaaag aagcccacga gcaaaacgct cccggctgca accagattgg
      121 gtttttgatg ctgtaaaatg agtgccaaaa aaataagcac ggttaacacc agtaaataac
      181 gatggctaat aattctgagt aatatatccc cccatgaaat aaaaataaga aagagcgcaa
      241 caaataaaag atcgactctt agtaaccata ttgctgccat tgaaagactc catgtaccat
      301 acaaggttgc tgccagacat attcctttac gtctgacagc ctctagagag tttttttatt
      361 ttttataagt tacggattac tcgttccgga aatcgttacc tgttgaattg ctgcagcgat
      421 agcgtcgaat gcatctttaa ttgcgcccag gaagcctgag ttctgatcgc caaagatgaa
      481 agcaagcaga gttgccatag caacaccaat caatgcatat tcgattgcag taacaccacg
      541 ttcatccttc gcgaaacgcg caaagcactg tttagtttta ccatagtact tagtaataaa
      601 agtattcatg ttttcctcgt atattattat ttaaattcaa accaccagct tcatctgcgt
      661 gtcaccggta tcccatattt ttgggatgtg gctataatta tctattcccg ctatttagtc
      721 ttcaaaaaaa attacattta atatcattta tcttgattaa gaaatagtaa gactaaaaaa
      781 acaagcgtcg tcagccagca cactgctaac acggtaagaa cgctaataaa aacactccac
      841 tgcttcgttc tataccattc aatatttttg tcagtatgtt ttattgggag cagctcttca
      901 gtagagaatt cctggctgtt atcattctca ggaatgtttg catcagtgtt tacttccctg
      961 gcctcgctat cgttggtata gctactactg tcttcatcac tgtcacgctc attctcacag
     1021 acatgtttct cgacctttcc cagatatctg acgccttttc gcggaatggt tttaattaga
     1081 gaactcgaca aacctgcgac gtctaatgcc tttctaagca tgtaaatctg cccataataa
     1141 ctgctgtcgc ttaccgaacc tttctgctcc cgccaaacct cattgataat atgttgctta
     1201 tcggattctc cctgtagtaa aaggagtaaa aatcgatagc cgttttcaga cagattgatt
     1261 acattgcctg tactggtggt gatacgtcga gacgctgggt aaaacttaac gccctgccct
     1321 aaagaaaaat aaaaacggtc atcgctcata agtaccgacc cctgttttct tcttcacatg
     1381 actactcatt acaactttgt atgaagtaag atgcaaaatc atattgttta cattaagtta
     1441 ag
//
LOCUS       DAJTRN010000261         1438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTRN010000261 DAJTRN010000000
VERSION     DAJTRN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..543)
                     /locus_tag="OO328_004161"
                     /pseudo
     CDS             complement(1..543)
                     /locus_tag="OO328_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(591..1163)
                     /locus_tag="OO328_004162"
     CDS             complement(591..1163)
                     /locus_tag="OO328_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5932480.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          427 a          304 c          279 g          428 t
ORIGIN      
        1 ctctgctatc agatttcgcc cttcccggcg ccatttcact ttcggcgcag catcttcggg
       61 aataaggcgg atatttgctg gtcgccagaa cagcttgata cgcgttttta ctgctatttc
      121 cagcgagttc gtcgctttgt tgctcgctga tggcggaata tttttaatat tcagccagaa
      181 gactgactcg cgatcatcag gcaatgacac acctttggtg tgaataatgc gtaatatttt
      241 ttcttgcgaa ggttctaatc gtgatactgg cggaataacg gtaaacggcg ctttgctttt
      301 atcctcttta ttaaatggat ctacccaagc ctggatgaga taaggtattt tttcttcttt
      361 gctaaataca gaaatagatt tatcagcatt attacttaag taaaccaccc gcgtactacc
      421 aatgacaacg cctgcataag ttaatgctgg ttggcctaaa aaaacgctga gtataatcgc
      481 ggtaaataac ttacattaaa ctgttgtttt aaagagcctg attaaacaac tttttctgga
      541 cataacgact ccctggccaa agccaactga ataaaaaggc ccttgataaa ttatttctta
      601 tattcgacag taaattgtga atcagcgttc gccgtaccag ccgttacggt cgcattggtt
      661 gccacatagc gtgcaataaa cttcatttca gccttgcctt cagcatcgat agcggtataa
      721 gcagagtcgt tataaagttt aacctgcgaa ttatcggact ggttaaaaat acgaatacca
      781 acgccggttg ccgcaatcgc ttgtcctgta ccggtaaaat caccagggtt gcctgctgta
      841 agcggcgtac ccactttcag gtcgccgtcg ccgccaggcg cttctgtacc atcaaaacga
      901 acggctgctt gagtgtacgt tgccgggcaa ttttcaagtt taatactgaa cttctgtggc
      961 gacgccagac tacctacacc tgaaaatgca tttttactga ctttacccag tgggacaatt
     1021 tgatcggctg tatcggaggt aacttcacaa gtagtgtcga caatttcacc gacgaagtta
     1081 acaacaccat cttctgccag tgcatgcccg gccagcatta gagatgccag agtgaatgca
     1141 ccgcctataa tattacgctt cataaaaact ccattgatat aatatatcct ttaccatgaa
     1201 tcatcactta tattaataag tgtgtttttg attcaaagga atagtacacc aaagagacgg
     1261 ctttatctaa aaataatatt aattacgcaa aaataaaata tagatttatt gtttaaatat
     1321 tatttaaaca gtaaaaaatc aagtttgtta tcagacatta atgataattc agaatatatt
     1381 tatatagaaa gatagatgaa tttattgatg tcagttataa aaaagggcca ccggtcgg
//
LOCUS       DAJTRN010000262         1433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRN010000262 DAJTRN010000000
VERSION     DAJTRN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            82..1275
                     /locus_tag="OO328_004163"
     CDS             82..1275
                     /locus_tag="OO328_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5932481.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1433)
                     /locus_tag="OO328_004164"
                     /pseudo
     CDS             complement(1295..1433)
                     /locus_tag="OO328_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          421 a          309 c          336 g          367 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aac
//
LOCUS       DAJTRN010000263         1405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRN010000263 DAJTRN010000000
VERSION     DAJTRN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..168
                     /locus_tag="OO328_004165"
     CDS             <1..168
                     /locus_tag="OO328_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5932482.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            153..791
                     /locus_tag="OO328_004166"
     CDS             153..791
                     /locus_tag="OO328_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5932483.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..>1405
                     /locus_tag="OO328_004167"
     CDS             1019..>1405
                     /locus_tag="OO328_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932484.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          394 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgatttct ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ctctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat gcaggaaaac gcctgagggg ctggttgcct gtagcctgca
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa gatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagt
//
LOCUS       DAJTRN010000264         1368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRN010000264 DAJTRN010000000
VERSION     DAJTRN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            602..886
                     /locus_tag="OO328_004168"
     CDS             602..886
                     /locus_tag="OO328_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5932485.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            886..1161
                     /locus_tag="OO328_004169"
     CDS             886..1161
                     /locus_tag="OO328_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5932486.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          367 a          343 c          341 g          317 t
ORIGIN      
        1 cagataatcg acaacaagtc ccattcccag tgtcgcaaca attgagccga agatatagga
       61 aaatgtgcct gacagcccta acagaataag tttacgcgta aatcgccctg aatccagaag
      121 agcattaagt atcacataaa caggttccat accagtcctg accgcatcag gacgccagta
      181 aataaaatct cgtagtccac cggcatcaga gacaagagta atcgtcgtta ccggtatttc
      241 gactggtttg tactggtgtg agcggacaaa actactgtac gtctctcata cacgctcgta
      301 agcggccctc acggcccgct aacgcggaga tacgccccga ctgcgggtaa acccttgtcg
      361 ggaccactcc gaccgcgcac agaggcattt tcatcgctga caggccgcat ggcctcgcag
      421 agccggaggg ggactggtga tattcggacg accgtaccgg cttgcgccgg gcttcggcgg
      481 ttttacacct gtgctacatg taaccaccgt atgccgcctt atatgcgctg actcgcatta
      541 tttgccttgt tccgtctgaa agtaatcact atgattaaat atgattaaca actaatcggg
      601 tatgcaaatg aaaaacaata ccgcacaagc aacaaaagta attaccgcgc atgtgccatt
      661 acctatggct gataaagtcg accagatggc cgccagactg gaacgctccc ggggctgggt
      721 tatcaaacag gcgctttctg catggcttgc ccaagaggag gagcgtaatc gcctgacgct
      781 ggaagccctg gacgatgtga catccggaca ggttatcgac catcaggctg tacagtcctg
      841 ggcggacagc ctcagtactg accatctgtt accggtgcca cgctgatgga actgaagtgg
      901 accagtaagg cactttctga tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa
      961 cctgctgccg ccagaacggt gcagtccctg acacaggcgc cggtcattct gttaactcat
     1021 ccacgtatgg gagaacagtt gtttcagttt gaacccaggg aggtcagacg gatttttact
     1081 ggcgagtacg aaatccgtta cgaacttact ggccagacta tttatgtatt gcgcctgtgg
     1141 cacacacgag agaacaggta gattgggtgt ggaggcaact ttaacatagc ctgcaggtat
     1201 taattctgga gcattgttat caggccagat attttgcatt ccattaaata atcttgtagg
     1261 agataaaata aattacgatg aaataacgaa aattaccgct cgtgactggc ggcaatatcg
     1321 cgctccgggc tggcaaataa cccatcagaa acgttattgc cagacatt
//
LOCUS       DAJTRN010000265         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRN010000265 DAJTRN010000000
VERSION     DAJTRN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            400..1149
                     /locus_tag="OO328_004170"
     CDS             400..1149
                     /locus_tag="OO328_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5932487.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTRN010000266         1337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRN010000266 DAJTRN010000000
VERSION     DAJTRN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            292..>1337
                     /locus_tag="OO328_004171"
     CDS             292..>1337
                     /locus_tag="OO328_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5932488.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          436 a          297 c          215 g          389 t
ORIGIN      
        1 tgattattag caacaaatat taccaagtca agtagtcagg ctggctaaaa tagttggaga
       61 tgttgtagta tcttactcaa gactaatgtc ttctggtata aatcctctgc attgtgaata
      121 tgtaaaacat caaaaaaaga tgaaccaaat atacgaaaaa agcaaatata cttaccaggg
      181 tagaaagcat gatataacac tcattccctt atggcaaata ttctgaaaca ccctcctcat
      241 taggagagat acattaaata caatttattc acaatctaat tccatatcca tatgcgagaa
      301 attaatatgc tcagaaacat ttcatcctgt ttatttccac atatcagcac aattacatcc
      361 cccaaccatt atttgtccga atgggatgat tgggagaaac aggggttacc ggaagaacag
      421 cgtactgagg cggtaagaag acttcgtgca tgtcttacct ctaaggggca taaactggac
      481 ctgcgagcct tggcgctttc ctcgctacct gtactccctg cttgcattaa aaagcttgat
      541 gtgagctgta ataaattaac catccttact gatttacctg aaaatattaa agaacttatt
      601 gcaagagata atttcttaac acatatatct gcattaccac attatctaat aactttggat
      661 gtgtccgaaa atcaattaga gaatctgccg ttattaccag acaccatcaa atcactaagc
      721 gcagagtata ataggttatc cacactgcct tcattaccct tgaatttaaa aaaacttgag
      781 gttaggaaca acgaactgca aactcttcca tctctgcctt ctaatcttaa gatacttaag
      841 gttgcgcaca accatcttac tgaactgccc cctttaccta ggagactgca acttcttttt
      901 gcatatagca atagattaag caacttacca aacatccaag aaaatattat catgagaaga
      961 tttttttatt ttgaaaacaa ccaaataact acaatcccga caaatctttt tcgtttagat
     1021 cctcatataa ctattgagat tgcaaataac cccttatcag atcaaactct gctattctta
     1081 atacagcaaa cttcggttcc aaattttaac gggcctcagt ttcgtatttc cctgtcagac
     1141 caaaacagac tgtttttacg ccagatgttg ccgcaaaatt tacattcgcg ccatatcaga
     1201 gtcatcactg aaggggggca gaactttcag atcccccctc ttcccgaaac tgtggcagcc
     1261 tggtttcctg aagcagatcg tcgggaggtt tctacacaat ggacttcttt ttccaccgag
     1321 gagaattccc gggcatt
//
LOCUS       DAJTRN010000267         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRN010000267 DAJTRN010000000
VERSION     DAJTRN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO328_004172"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO328_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5932489.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTRN010000268         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRN010000268 DAJTRN010000000
VERSION     DAJTRN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            34..435
                     /locus_tag="OO328_004173"
     CDS             34..435
                     /locus_tag="OO328_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5932490.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO328_004174"
     CDS             447..818
                     /locus_tag="OO328_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5932491.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO328_004175"
     CDS             805..>1275
                     /locus_tag="OO328_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5932492.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTRN010000269         1259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRN010000269 DAJTRN010000000
VERSION     DAJTRN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..705)
                     /locus_tag="OO328_004176"
     CDS             complement(<1..705)
                     /locus_tag="OO328_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5932493.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIP"
BASE COUNT          332 a          194 c          312 g          421 t
ORIGIN      
        1 cgggatatga cttatctgat ttatgtcaaa ataaccgttc cttaaatttt ccggcaggta
       61 tggaatatcg gttaacatat tgtcgattgc ccatatggag tgaatgttgt gaggtagagg
      121 ggggagttca ctaagaagat tccctattgc gctaatctct tgtaaagaaa atggtaaagg
      181 tggtaaaact gccagcccat tacatgatac atctaatgtt tccagtagtt ctggtaaaac
      241 aggaagagaa cataattgat tatttgagac atgaagctcc ttcaaagata tggggagtgt
      301 gggtagtgtg attagttgat tgtgggacgc attcaataat ttaagtcctt gaggcaaagc
      361 aggcagttca ataagtctgt tatagctgac attaagttgt gtaagggacg caggcaatgg
      421 ggagattaag cttaaattat ttttacttat attaattgat ttaattcccg gggggatttc
      481 aggtaatgtt gtcaggccta attcagacaa gtccaggttc gtctcttggt tttgtagaca
      541 tgatactagt cgctgaaaag cgatgtctcg ttgttcttct tgtatgcggt tatttttcca
      601 ttcagtccaa tgggttagat aattttcata tgcgcggcta gtgtcgattg aataagtgga
      661 gaacgaattt gaaattaatc tgtgattgtt atttgtcggg agcataaata tcagggtgta
      721 ttctttatgt tgagaggtgt tggattcttt tttatctgct ttgcaatgtt attggttccc
      781 ttcagcagta gacaggaact tctggaggag gcggatatat agtttgtgta agcatattaa
      841 ctccatatgt tatatattga agaacttctg ctttacacta tcctaaactg gatcggttta
      901 atgtaaaaaa acgatacttt ttgggggggg gcaatttttc cttaaggtat gaaaaaagga
      961 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1021 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1081 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1141 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1201 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtg
//
LOCUS       DAJTRN010000270         1258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTRN010000270 DAJTRN010000000
VERSION     DAJTRN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..1018
                     /locus_tag="OO328_004177"
     CDS             <1..1018
                     /locus_tag="OO328_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5932494.1"
                     /translation="DNYTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWD
                     IFADQTSMITAGYNRYYGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELA
                     MGLQQKIDKNVIARASEAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAE
                     PLHIRQVDINPQIVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFN
                     NPLKISLNMDFTHQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKL
                     DSSLTWDTRLSWTPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDV
                     SMKF"
     gene            1075..>1258
                     /locus_tag="OO328_004178"
     CDS             1075..>1258
                     /locus_tag="OO328_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932495.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIH"
BASE COUNT          373 a          281 c          283 g          321 t
ORIGIN      
        1 tgacaactac acgctgtata tggcggatca cattagctgg cggaatgtgt cgttaatgcc
       61 cggtgtgcgt tatgactatg acaactatct gtcaaaccac aatatctccc cgcgctttat
      121 gacggaatgg gatatttttg ctgatcaaac ctcaatgatt accgccggtt ataaccgtta
      181 ctatggcggg aatattcttg atatgggatt acgtgatatc cgcaatagct ggacggaatc
      241 ggtatcaggt aataaaaccc tgacgcgtta tcagaatttg aaaacgcctt ataacgatga
      301 actggcaatg ggattgcagc aaaaaatcga taagaacgtt attgcacgcg caagtgaagc
      361 gcatgatcaa atcagcaaaa gcagtcgtac cgacagcgcg actaaaacca ccattactga
      421 atataacaac gatggcaaaa ccaaaacgca ttcgtttaac ctcagttttg aactggccga
      481 acccctgcat atccgccagg tagatattaa cccgcaaatt gtctttagct atatcaagag
      541 caagggcaac ttgtcgttaa acaatggtta tgaggagagc aataccggtg ataaccaggt
      601 ggtttataac ggtaatctgg tctcttacga tagcgttcca gtggcagatt ttaataaccc
      661 attaaagatc tccttaaaca tggatttcac gcatcaaccg agcgggttag tgtgggcgaa
      721 tacgctggcc tggcaagaag cgcgtaaagc tcgcattatc cttggtaaga caaatgcgca
      781 atacatcagc gaatattcag attacaagca gtatgttgac gaaaaactgg atagcagcct
      841 gacctgggac acccgcttgt cctggacgcc acaatttctg aaacaacaaa acctgacgat
      901 cagtgccgat attctcaatg tactggatag caaaaccgct gttgatacaa cgaacaccgg
      961 tgtggcgacc tacgccagtg gccgtacttt ctggcttgat gtcagcatga aattttaata
     1021 gttcggattt tacttaaccg ggtaaattcg cccggttttc gcatggagat aattatgaga
     1081 aacctctgtt tcttactgac gttagtggca actctgttgc tccccgggcg gctgattgcc
     1141 gccgccttac cgcaggatga aaagttaatt accgggcaac tggacaatga cttgcgatat
     1201 atgatttatc cgcatgctca tccaaaggat caggtaaatt tatggctgca aattcata
//
LOCUS       DAJTRN010000271         1226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTRN010000271 DAJTRN010000000
VERSION     DAJTRN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            114..>1226
                     /locus_tag="OO328_004179"
     CDS             114..>1226
                     /locus_tag="OO328_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5932496.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQ"
BASE COUNT          350 a          299 c          289 g          288 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaa
//
LOCUS       DAJTRN010000272         1224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTRN010000272 DAJTRN010000000
VERSION     DAJTRN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..114
                     /locus_tag="OO328_004180"
     CDS             <1..114
                     /locus_tag="OO328_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5932497.1"
                     /translation="RLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            229..>1224
                     /locus_tag="OO328_004181"
     CDS             229..>1224
                     /locus_tag="OO328_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5932498.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSP"
BASE COUNT          334 a          316 c          324 g          250 t
ORIGIN      
        1 cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag aaattacctg
       61 gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa ttaagggaca
      121 cagcctaaat agctgcgcgg aatagtagat ccctttgagg gaacttagcc cggattgtgc
      181 gatctgatca atcgccaaat caaaacaaat caccaaccgg actgagcaat gccgatcata
      241 gcaccaattt cccgtgacga acgacgcctg atgcagaaag ccatccataa aacacacgat
      301 aaaaattatg cccgcagact gactgccatg ctgatgctgc accggggcga ccgtgtcagc
      361 gacgttgcca gaacgctctg ctgcgcccgt tcctctgttg gacgctggat taactggttc
      421 acgcagtcgg gtgttgaggg actgaaatca ttacctgccg ggcgtgcccg tcgctggccg
      481 tttgagcata tctgcacact gttacgtgag ctggtaaaac attctcccgg cgactttggc
      541 taccagcgtt cacgctggag tacagaactg ctggcaataa aaatcaatga gataaccggc
      601 tgccagttaa atgccggaac cgttcgccgc tggttgccgt ctgcggggat tgtgtggcga
      661 agggctgcgc caactctgcg tatccgtgac ccgcataaag atgaaaagat ggcagcaatc
      721 cataaagcac tggacgaatg cagcgcagag catccggtct tttatgaaga tgaagtggat
      781 atccatcttc atcccaaaat cggtgcggac tggcaactgc gcggacagca aaaacgggtg
      841 gtcacgccgg gacagaatga aaaatattat ctggccggag cgctgcacag cgggacaggt
      901 aaagtcagct atgtgggcgg caacagcaaa agttcggcgc tgttcatcag cctgctgaag
      961 cggcttaaag cgacataccg tcgtgcgaaa accatcacgc tgatcgtgga caactacatt
     1021 atccacaaaa gccgggaaac acagagctgg ctgaaggaga acccgaagtt caggggcatt
     1081 tatcagccgg tttactcgcc atgggtgaat catgttgaac ggctatggca ggcacttcac
     1141 gacacaataa cgcgtaatca tcagtgccgc tcaatgtggc aactgttgaa aaaagttcgc
     1201 cattttatgg aaaccgtcag ccca
//
LOCUS       DAJTRN010000273         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTRN010000273 DAJTRN010000000
VERSION     DAJTRN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..263)
                     /locus_tag="OO328_004182"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO328_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO328_004183"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO328_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5932499.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          353 a          290 c          183 g          364 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg
//
LOCUS       DAJTRN010000274         1141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTRN010000274 DAJTRN010000000
VERSION     DAJTRN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..848
                     /locus_tag="OO328_004184"
     CDS             <1..848
                     /locus_tag="OO328_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5932500.1"
                     /translation="LILITLLRWIQHLTSLGRVGETTAQVEQAAIETFIARARNPCLG
                     GYPWLENNEQPKGTVAVYPKKIGYVEYIDMVKLSKLLTNDPRHVYLVAQPGSFIHPSM
                     PVLYLSQGQESSINTDLLETIIVSDARSFAQDPRFCLSVMAEIACRALSPAVNDPGTA
                     IDVIGRGVRILSTYAQNKSDEIEVKYPSVHVAPLQNNDLLEDFFSPVARDGASMREIQ
                     IRVLKGLSMLSKGWPEIFAEAAHTLAFETLEHATRADHIDSDRYLIKSTYYNLFSGEY
                     SNKKT"
BASE COUNT          318 a          250 c          272 g          301 t
ORIGIN      
        1 ccttaatcct catcacattg cttcgctgga tacagcattt gacctctctg gggagggttg
       61 gtgagacaac ggcacaagta gaacaggcgg ccatcgaaac atttattgcg agagcaagaa
      121 atccctgtct cggtggatat ccatggcttg agaacaatga acagccgaaa ggaacggttg
      181 cagtttatcc gaagaagatt ggctatgttg aatatattga tatggtgaaa cttagcaagc
      241 tgctgaccaa tgatccccgt catgtatacc tcgtggcgca gccaggcagt ttcatacatc
      301 cgtccatgcc agttttgtac ctgagtcagg gccaggagtc gtcaatcaac accgatttac
      361 ttgagacgat tattgtctcg gatgcacgtt catttgctca ggatcctcga ttttgtctta
      421 gcgtcatggc cgaaatagcc tgccgggccc tttcccctgc agtgaacgat cctggaaccg
      481 ccattgatgt cattggcaga ggtgttcgta tactttccac ttacgcgcag aataaatctg
      541 atgaaataga agtgaaatat ccttcagtac atgttgcacc acttcagaat aacgatctac
      601 tggaagactt tttctcacct gtcgcgcgcg atggtgccag tatgagggag attcagataa
      661 gagtccttaa aggactgtcg atgctgagta aaggttggcc tgagatattt gctgaagccg
      721 cacataccct ggcatttgaa acattagagc atgcaactcg tgctgaccat atagattctg
      781 atagatatct aataaaatca acttattata atttatttag tggcgaatat tctaataaaa
      841 aaacatagct gcagagcaag caattcggtt gttggcaagt caagggcaaa gcattcgcgt
      901 gagaatgggc gtatgctttg tgccaaaagc aaacgttgct aattctgcac tgttcagcga
      961 tggcgcagta gagacaggca ctgccactgt ccagtcggca aaagcgaaca ctttgccact
     1021 gcggggataa actgattgaa gctctcgcgc aaaatcactt attcttttgc gttaataaaa
     1081 tgtaggagat gggtcattga tcctacccac gtaatatgga cacaggccta agcgaggttc
     1141 t
//
LOCUS       DAJTRN010000275         1139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTRN010000275 DAJTRN010000000
VERSION     DAJTRN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..231)
                     /locus_tag="OO328_004185"
     CDS             complement(<1..231)
                     /locus_tag="OO328_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5932501.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRL"
BASE COUNT          327 a          272 c          250 g          290 t
ORIGIN      
        1 gagacgcaca gggttgctac aaccggtgac taccgaaacc ggccagcctt gcggctgccc
       61 tgcacgcccc accataatct gaatgtggct gtgcttgacg cataaaaaaa ccgcctgagc
      121 gcggttatgc gcagtagtca tcgtcggggt agcaatcccg gcacccgttt tatgaggtgc
      181 aggtgcacta taattccacc cgttctggtt ttcaatagct acattcaaca ttttctctta
      241 cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa
      301 ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac gtgggaacta
      361 atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca aaattcactt
      421 taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat
      481 ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc
      541 aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc
      601 ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa atttgtataa
      661 tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg tcagctctca
      721 aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct gaggttgatc
      781 gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat ttgtcatctc
      841 attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa actcacggag
      901 atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat tactggaacg
      961 attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta tttttgtcca
     1021 gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga ggaaggcgat
     1081 aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga ctacaattt
//
LOCUS       DAJTRN010000276         1123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTRN010000276 DAJTRN010000000
VERSION     DAJTRN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..859)
                     /locus_tag="OO328_004186"
     CDS             complement(<1..859)
                     /locus_tag="OO328_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5932502.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSV"
     gene            complement(1006..1082)
                     /locus_tag="OO328_004187"
     tRNA            complement(1006..1082)
                     /locus_tag="OO328_004187"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1046..1048),aa:Pro,seq:tgg)
BASE COUNT          285 a          303 c          272 g          263 t
ORIGIN      
        1 tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat
       61 tggcaaacgc ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt
      121 ggtcaaaaat cgccaccaga aattcaccaa attcatcggg gcgcacagac cattccgtca
      181 gttccccctg aatatcacca ggcgcatgta gtttcagtcc atcgcggaca gctgtttcat
      241 cagccaggcg ctcgaccacc ggaataaact ggatgaattc gactcccgca tcgcacaaaa
      301 aatcataaac ctgtaacggt tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat
      361 agtcgacatg atgtttttgc aggagcgtca gggcacgcat caccagttta tgcgtgggtc
      421 tgccgccttt ggtcacgcga tattgattgt ggatctcagc cggaccatcc agcgataacc
      481 caacaagaaa attattttct gccagaaatg cacaccattc gtcatcgagc agcacgccgt
      541 tagtctggaa actgttactt atcttcctgc cagcaccata tttcgcctgt agcgccacgg
      601 cacggcggta aaactccagc ccgagtagcg ttggttcgcc gccctgccag gtaaaagcca
      661 cttcgttttg cagttcgctg gcggcgatat agtgacggat atacgcttcc agcgtgtcat
      721 cgtccatatg cgtgactggc ttttcgcggt aaagggattg tttttcgaga taaaaacagt
      781 agtcacagtt cagattacaa tcggaaccgc tcggtttcgc tatcacatga aaagcacgcg
      841 ttggaacctg ttgcagcatg gtcgctcctt gcctgaagtc atccttcaat acgttaaggg
      901 cgaaatcata ccgtggggca atgagttatt acgggcgttt attgcgaagg gagggtgacg
      961 aaatgcacag aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag
     1021 gattcgaacc tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc
     1081 cgaaatactg ctttttgaat ttttagttca attctttaaa gtc
//
LOCUS       DAJTRN010000277         1102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTRN010000277 DAJTRN010000000
VERSION     DAJTRN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..954)
                     /gene="yjfF"
                     /locus_tag="OO328_004188"
                     /pseudo
     CDS             complement(1..954)
                     /gene="yjfF"
                     /locus_tag="OO328_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(941..>1102)
                     /locus_tag="OO328_004189"
     CDS             complement(941..>1102)
                     /locus_tag="OO328_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5932503.1"
                     /translation="ALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLI
                     KGVRSHDKT"
BASE COUNT          325 a          324 c          261 g          192 t
ORIGIN      
        1 acgattctcc cacagcaccg ttaacccacg ctgtaatgct ataaaaataa acaacaaaat
       61 accgatggcg attttcgtcc actaggaact cagcgtgccg tcaaagttga tgtaagtctg
      121 gatcagtccc tgaattgcca cgccaaaaag cgtcccaagc accgtcccga cgccaccact
      181 taacaacgta ccgccaatca ccaccgacgc gatggcgtcc agttccacac ctacgcccgc
      241 cagtgcatat ccggcttggg tataaatcga gaagacaatc cccgccagtg ttgccagtcc
      301 ggtggagagc atataaatgc gaatagtggt gctgcgagta gaaatcccca tcaggttcgc
      361 cgacgtcgcg ttgccgccaa tagcgtacac ctgattgcca aaacgggtac aatgcgcaag
      421 gaaaataccg ataaccacca ccgccagcat caacagcccc atcgcactaa ggcgaccgcc
      481 gccggggatt ttccacgcaa ggcttgagag cgtgtcataa atgggatggt ttatcggaat
      541 cgactcttcc gaaacgagat agctgacgcc gcgcaaaaag aacatccccg caagcgtaat
      601 gataaatgcc gggatcttca gggcgtcgat cagaagcccc ataaatgcgc cgaaggcaca
      661 gcccatcacc agcactagcg gaaacgccag cagcggcgag aggccgaaat cgccaaccac
      721 ttttgccaga aacacgccag taaaggcgat caccgaaccg acggagagat cgataccgcc
      781 agagaggatc acaaaggtca tgccaacggc gattatccca agaaaggcgt tatcggtcag
      841 gatattgcag atcacccttg tggaagcaaa accgggaaac tgggtcaggc agtagagata
      901 gcccagcaca aagacgccga tggtgatcat cagcggcaag ttacgtttta tcatggctac
      961 gtactccttt aatcagactg ataaagcgtt gcgactgaac aatcagcaca caaagcacca
     1021 ccaccgcttt caccacctgg ttcatctccg gcggaaagcc cgaaagcaaa attccggtgt
     1081 tcatcccctg aataatcagc gc
//
LOCUS       DAJTRN010000278         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRN010000278 DAJTRN010000000
VERSION     DAJTRN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO328_004190"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO328_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5932504.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          347 a          158 c          194 g          389 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt
     1081 gcttgcgt
//
LOCUS       DAJTRN010000279         1042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRN010000279 DAJTRN010000000
VERSION     DAJTRN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..342
                     /locus_tag="OO328_004191"
     CDS             <1..342
                     /locus_tag="OO328_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="3'-5'exoribonuclease that acts nonspecifically on
                     poly(A), poly(U) and ribosomal RNAs; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5932505.1"
                     /translation="RLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTAREKAKK
                     GDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAATK
                     AKRAAKKKVAE"
     gene            522..>1042
                     /gene="rlmB"
                     /locus_tag="OO328_004192"
     CDS             522..>1042
                     /gene="rlmB"
                     /locus_tag="OO328_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5932506.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGT"
BASE COUNT          295 a          259 c          297 g          191 t
ORIGIN      
        1 cgcctgggcg atcgcgtgga agttcgcgtc gaagcggtta atatggacga gcgcaaaatc
       61 gactttagtc tgatctccag tgaacgcgca ccgcgcaacg tcggtaaaac ggcgcgcgag
      121 aaagcgaaaa aaggcgatgc aggcaaaaaa ggcggcaagc gttgtcaggt cggtaaaaag
      181 gtaaactttg agccagacag cgccttccgc ggtgagaaaa aaacgaagcc gaaagcggcg
      241 aagaaagacg cgagaaaagc gaaaaagcca tcggcgaaaa cgcagaaaat agccgcagcg
      301 accaaagcga agcgtgcggc gaagaaaaaa gtggcagagt gatcaatacc ctctttaaaa
      361 gaagagggtt agattgctga caaaatgcgc tttgttcatg ccggatgcgg cgtgaacgcc
      421 ttatccggcc tacataatca cgcaaattca atatattgca gagatcatgt aggccggata
      481 aacgtagtgc atcaggcaaa acgtaaacaa cgagtacatt aatgagcgaa atgatttacg
      541 gcatccacgc agtgcaggcc ctgctggagc gcgcccctga acgttttcag gaagtcttta
      601 ttttaaaagg ccgtgaagat aaacgtctgt taccgctgat tcacgccctt gagtctcagg
      661 gcgtggttat ccagttggca aaccgccaat atctcgacga gaaaagcgac ggtgccgtgc
      721 atcagggcat tatcgcccgc gtgaagccag gacgtcagta tcaggaaaac gatctgccgg
      781 atctgatcgc ttcgctcgat caaccgttcc tgctgatcct cgacggcgta accgatccgc
      841 acaacctcgg cgcgtgcctg cgtagtgcgg acgccgcagg cgttcatgcg gtgattgtgc
      901 cgaaagatcg ctccgcacag ctcaacgcta cggcgaaaaa agtagcctgc ggcgcggcag
      961 aaagcgttcc gctgattcgg gtgactaacc ttgcgcgcac catgcgtatg ttgcaggaag
     1021 agaatatctg gatcgtcggt ac
//
LOCUS       DAJTRN010000280         1027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRN010000280 DAJTRN010000000
VERSION     DAJTRN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..297)
                     /locus_tag="OO328_004193"
     CDS             complement(<1..297)
                     /locus_tag="OO328_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932507.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            362..1010
                     /locus_tag="OO328_004194"
                     /pseudo
     CDS             362..1010
                     /locus_tag="OO328_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          273 a          215 c          200 g          339 t
ORIGIN      
        1 tttcaggcgc ttcgcgaagt atgtcgcggc cttttggagg atggacagtt cctcagcctg
       61 ctccgccagt tgtcgtttaa ggcggacatt ttcagcggcc agttcgcttt cgcgctctga
      121 cgaactcatt tgttgctgct gtttactgcg ccaggcataa agctgagatt catacaggct
      181 gagttcacgg gctgcggcgg ccacaccgat gcgttcagcg agtttcaggg cttcgttacg
      241 aaattcaggc gtatgttgtt tacggggctt cttgctgatt gatactggtt ttgtcatgag
      301 tcacctctgg ttgagagttt actcacttag tcctgtgtcc actattggtg ggtaagatca
      361 ccctccatta aaactaaaac gggcgctttc tgatatatca tctgtaagcg cagtaatacc
      421 attagtagca cctcgcgcat tgattattga atattataac tggaatgtta tatttcttgt
      481 tatgatgctg ttcagtatta taataatgtt attatcacca aagataactc atgcaaatga
      541 gaaagtaata tgtcaaaaca gtgcatcctt tgtgaccaaa gagtttatct gcggcactat
      601 acttgtctca ttaatactca gttctctatt ttgctacata tcaatatcac catcaatttt
      661 catgcatact tatggcctga gtacttttag ttactcaatt attttctcct tctcagtttt
      721 atttttcata atcggaaatc aattatcaaa gattgaaaaa ttatccaacc tatggttttt
      781 attacccaat agccaatttt atcgcattac aaataaatgg ttcaaaaaca ggatttgccg
      841 catctattac aggttttact caaacaataa gcgctggtac aatcagtttc ctgtcagtaa
      901 ccgtaagcga caacggtgtt ggtcccggaa ctactttagg tcttggttca ctaataactt
      961 cattactgtc aatagccgta atcgccattg caggaaataa taaaaagtaa gtgtactctg
     1021 actgcgt
//
LOCUS       DAJTRN010000281         1007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRN010000281 DAJTRN010000000
VERSION     DAJTRN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(237..395)
                     /locus_tag="OO328_004195"
     CDS             complement(237..395)
                     /locus_tag="OO328_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5932508.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            complement(379..690)
                     /locus_tag="OO328_004196"
     CDS             complement(379..690)
                     /locus_tag="OO328_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5932509.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(874..>1007)
                     /locus_tag="OO328_004197"
     CDS             complement(874..>1007)
                     /locus_tag="OO328_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="converts protoheme IX and farnesyl diphosphate to
                     heme O; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5932510.1"
                     /translation="VADDRIWARKLFGFSIIAITALSVMMSVDFMVPDSHTLLAAVW"
BASE COUNT          267 a          238 c          220 g          282 t
ORIGIN      
        1 gaattttttt tacatgttca aaaaaatcac atagtaggtc ctgaccaaag ccccgctttt
       61 gatacttccg cgctcccccc aacatgacta atctgacaac accaatttct gaaggttgtg
      121 aaccctgaag gacgccagaa acgcgttgtc tttccagcga atatgccgta aaagtacaga
      181 tgccaatcag ttcaccactt tgtctgtcaa taagtgcttt agccgcacag tcgctattac
      241 gcacactttt ctttagcgat gcgcgtagcg cgtacaaatt tatcgataac agggttaccg
      301 caatcaaact gtttttgccc aggataagta atatcagact gataattaca ggagaggact
      361 atgggcagag taatctcttc atgcttgtcg accatcgctg tttttccttt tcattaacgc
      421 cttgagggcg agcgtcggtt gagcaggttc agcgatgagt tggttcatca gttcccagct
      481 ttgattcgaa agctcacggc gacgttggtt atcaagcaca ctttcggcgc gttccatagc
      541 agcgctcaaa ataaaagcgc ttaaatctac cccaacggct tgcgcagctt cacgaagttt
      601 gtttttggcg tcgggactag ttttgagttc gacccgcgca tttttcagcg ttttatcgac
      661 atcgtataga gcggctgttt ttccaggcat tatgacttct ttgattaact gaatgaaatc
      721 gtagggagtc gatctcatac ataatacggc tatcaaccgt atgattaatt tatgtgcatc
      781 tgctcactct gtcaagagat acggttatta gccgtatctc tgatctcaga caataaaaag
      841 gtgccgtagc acctttttaa tagagaggtt ttgttaccac acagcagcca gcagcgtatg
      901 cgagtccggt accataaaat caacggacat catcaccgag agggcagtga tggcgatgat
      961 agagaagccg aacagcttgc gcgcccagat tctgtcatca gcaactt
//
LOCUS       DAJTRN010000282         1007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRN010000282 DAJTRN010000000
VERSION     DAJTRN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..989
                     /gene="fbp"
                     /locus_tag="OO328_004198"
     CDS             <1..989
                     /gene="fbp"
                     /locus_tag="OO328_004198"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5932511.1"
                     /translation="GEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAGLVDI
                     LGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEHAKYV
                     VLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVVYGSS
                     TMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVKKYIK
                     FCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYECNPMA
                     FLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
BASE COUNT          246 a          262 c          259 g          240 t
ORIGIN      
        1 taggtgaatt tattgtcgaa aagcagcacg agttttctca tgctaccggt gagctcactg
       61 ctttgctgtc ggcaataaaa ctgggcgcca agattatcca tcgcgatatc aacaaagcag
      121 gactggttga tatcctgggt gccagcggtg ctgagaacgt gcagggcgag gttcagcaaa
      181 aactcgactt gttcgctaat gaaaaactga aagccgcact gaaagcacgc gatatcgttg
      241 cggggattgc ctctgaagaa gaagatgaga ttgtcgtctt tgaaggctgt gaacacgcaa
      301 aatacgtggt gctgatggac cccctggatg gctcgtccaa catcgatgtt aacgtctctg
      361 tcggtaccat tttctccatc taccgccgcg ttacgcctgt tggcacgccg gtaacggaag
      421 aagatttcct ccagcctggt aacaaacagg ttgcggcagg ttacgtggta tacggctcct
      481 ctaccatgct ggtttacacc accggatgcg gtgttcacgc ctttacttac gatccttcgc
      541 tgggcgtttt ctgcctgtgc caggaacgga tgcgcttccc ggagaaaggc aaaacctact
      601 ccatcaacga aggaaactac attaagtttc cgaacggggt gaagaagtac attaaattct
      661 gccaggaaga agataaatcc accaatcgcc cgtatacctc gcgttatatc ggttcactgg
      721 ttgcggattt ccaccgtaac ctgctgaaag gcggtattta tctctaccca agcaccgcca
      781 gccacccgga cggcaaactg cgtttgcttt atgagtgcaa cccgatggca ttcctggcgg
      841 aacaagcggg cggtaaagcg agcgatggca aagagcgtat tctggatatc atcccggaaa
      901 ctctgcacca gcgccgttcg ttctttgtcg gcaacgacca tatggttgaa gatgtcgaac
      961 gctttatccg tgagttcccg gacgcgtaat ctcctgcgcc tctccac
//
LOCUS       DAJTRN010000283         1003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRN010000283 DAJTRN010000000
VERSION     DAJTRN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            37..285
                     /locus_tag="OO328_004199"
                     /pseudo
     CDS             37..285
                     /locus_tag="OO328_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(332..>1003)
                     /gene="deoR"
                     /locus_tag="OO328_004200"
     CDS             complement(332..>1003)
                     /gene="deoR"
                     /locus_tag="OO328_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5932512.1"
                     /translation="LLGVSEMTIRRDLNNHSAPVVLLGGYIVLEPRSASHYLLSDQKS
                     RLVEEKRRAAKLAATLVEPDQTLFFDCGTTTPWIIEAIDNEIPFTAVCYSLNTFLALK
                     EKPHCRAFLCGGEFHASNAIFKPIDFQQTLNNFCPDIAFYSAAGVHVSKGATCFNLEE
                     LPVKHWAMSMAQKHVLVVDHSKFGKVRPARMGDLKRFDIVVSDCCPEDEYVKYAQTHR
                     IKLMY"
BASE COUNT          263 a          252 c          251 g          237 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaccg tgaccatacc gcgtgggcag
       61 ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc agcttaccgc accgctgaaa
      121 aaaggtcagg ttgtcgggac cattgatttc cagcttaacg gtaaatccat tgagcagcgt
      181 ccgctgatcg tgatggaaaa tgtggaagag ggcggattct ttggtaggat gtgggatttc
      241 gtgatgatga aattccatca gtggttcggc agctggttct cttaatcttc tgataaccgg
      301 atggcgcgaa acgtcatccg gttatacgtc attaatacat caacttaata cggtgcgtct
      361 gcgcgtactt cacatactca tcttccgggc aacaatcact caccacaata tcaaagcgtt
      421 tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt actgtggtcg acaaccagca
      481 catgcttttg cgccatcgac atggcccagt gttttaccgg caactcttca agattaaaac
      541 aggtagcgcc tttactgaca tgcacgcccg ccgcggaata aaaagcgata tccgggcaaa
      601 aattattcaa cgtttgctga aaatcgatgg gtttgaaaat ggcgttgctg gcgtgaaatt
      661 caccaccgca cagaaatgcg cggcaatggg gtttctcttt cagcgccaga aaggtattta
      721 gcgaataaca aacggcggta aaagggattt cattatcaat cgcttcaata atccacggcg
      781 tggtggtgcc acagtcaaaa aagagggtct gatcgggttc taccagtgtc gcagccagtt
      841 ttgccgcccg gcgtttttct tccaccaggc gggatttttg atcgcttaac aggtaatggc
      901 tggcactgcg cggttccaga acaatatagc cgccgagcaa aacgacgggc gcactgtggt
      961 tgttcagatc gcgacgaatc gtcatctccg aaaccccaag cag
//
LOCUS       DAJTRN010000284          993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRN010000284 DAJTRN010000000
VERSION     DAJTRN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            144..494
                     /locus_tag="OO328_004201"
     CDS             144..494
                     /locus_tag="OO328_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5932513.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            547..942
                     /locus_tag="OO328_004202"
     CDS             547..942
                     /locus_tag="OO328_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5932514.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
BASE COUNT          227 a          236 c          227 g          303 t
ORIGIN      
        1 ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact catagctaac
       61 cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta ggaaccatct
      121 gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt catgcatcac
      181 gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg
      241 ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa
      301 accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag accactacgc
      361 cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta cataccctgt
      421 gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt ccacagcaat
      481 cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat tttaaaggga
      541 gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat gaagttctct
      601 tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg tggttttagc
      661 gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc catcgcgatt
      721 gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca tatttgcggc
      781 atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg tctgtttcgc
      841 tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat
      901 ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat acgccacttt
      961 ctttttcccg gatttttacg ttatgtctca ccg
//
LOCUS       DAJTRN010000285          970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRN010000285 DAJTRN010000000
VERSION     DAJTRN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..363)
                     /locus_tag="OO328_004203"
     CDS             complement(<1..363)
                     /locus_tag="OO328_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5932515.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFQNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLR"
     gene            complement(431..>970)
                     /locus_tag="OO328_004204"
     CDS             complement(431..>970)
                     /locus_tag="OO328_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR5932516.1"
                     /translation="DYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALR
                     IKLSNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDE
                     KAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIK
                     RGQNNLWNNGGIQYAPPVR"
BASE COUNT          275 a          248 c          224 g          223 t
ORIGIN      
        1 tcgtagcagc cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc
       61 gaacacaata cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg
      121 tccgtaggtg tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc
      181 gcgatccaga aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt
      241 ttgaaataac caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca
      301 ggcacgaacc gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgagg
      361 catagaaacc tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc
      421 tcacaccgac ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat
      481 tttgcccacg tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg
      541 agtagttact cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct
      601 tgccgtaatc gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg
      661 ctttttcatc gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa
      721 gcgtccagcg tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg
      781 gttctttaga gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc
      841 gcaaggcata cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg
      901 ccttcgctga ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct
      961 tgaaatagtc
//
LOCUS       DAJTRN010000286          964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRN010000286 DAJTRN010000000
VERSION     DAJTRN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            266..>964
                     /locus_tag="OO328_004205"
     CDS             266..>964
                     /locus_tag="OO328_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5932517.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGL"
BASE COUNT          295 a          228 c          218 g          223 t
ORIGIN      
        1 cccattcact gtggttgata tcgacggtcc atcaggcaac gttaaaacac ttaacgatgg
       61 tgtaaaaaaa atgtgtctgg tgcacatagg aaagaatctg cccgcagaaa aaaaagccga
      121 atttctggca actctgattg caatgaaatt aaaaggtgaa atctgaaaaa aagaaagcct
      181 gcacactgtg caggcctgaa tgaagaacct gggacattta ttcatcactc gcagtaattt
      241 taatctgagt tgaggttaaa aaacaatgaa caccgataaa caagtttacc cactgtatta
      301 cgaagcaaaa aatgacaaag taagaaaacg tctcggtatt aaaggcggtt tttactgggc
      361 tgaagcgaaa aaattatcca ttgccatctc ccgtggtgct gttgcgattg acgatgcagg
      421 ctacgatgaa gatgacttta aaaaacctgt tcgcgtcaat ttgcccgttg ttgatgacct
      481 cccgccagaa ggcgtatttg atacggaatt ctgcaaccgt tacgaaaaag gcggggaaga
      541 tggcatcaca atggtattta tcgcatcttc tccctctgtt caggacaaac cggccagcac
      601 tgacaatacc aacgtcaacg gcgaagacat gacagaaatt gaggagaaca tgctcctgcc
      661 agtttccggt caggaactgc ccattcgctg gcttgcgcaa cacggcagcg aaaaaccagt
      721 aacgcacgtt tcacgcgacg aactccaggc attacacatt gctcgggatg aagaactacc
      781 agctgttact gccctggctg tttcccacaa aaccagcctg ctcgatccgc tggaaattcg
      841 cgatctccac aaactggtgc gtgatactga cagagttttc cctaatccag gcaattcaag
      901 cctggggctg atgactgctt ttttcgaagc atacatggac gcagactaca ccgatcgcgg
      961 tctg
//
LOCUS       DAJTRN010000287          950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRN010000287 DAJTRN010000000
VERSION     DAJTRN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..511
                     /locus_tag="OO328_004206"
     CDS             <1..511
                     /locus_tag="OO328_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5932518.1"
                     /translation="KTAEQYQMTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGD
                     GRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERD
                     ALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIE
                     WPASPEGSS"
     gene            535..>950
                     /locus_tag="OO328_004207"
     CDS             535..>950
                     /locus_tag="OO328_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5932519.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYAR"
BASE COUNT          262 a          214 c          270 g          204 t
ORIGIN      
        1 gaaaacagca gaacaatatc agatgacaaa acgatattcg gtcacctggc ttttttcaga
       61 agacggaaaa aactggtatg aagagctgaa gaatttcgcc agcgacacaa taaaaatagc
      121 ttacaccgga gatggtcgcg tggtgtgggt cggtaaggat gtgacaggca tcgagccacg
      181 caatgccagc gttatcgaag ttccggatat taccgctaac cgacggatta ccgcgccggg
      241 ttactggttt taccgcaatg atgaatttgt ctttgactac agactcaaag cggaagatga
      301 gcgtgatgcc cttctggctc aggtcagtgc ccggacaggg gaatgggaag aagacctgct
      361 gctggggtta atcagcgacg aagataaaga aaagctgaaa gcctgtcgta tttacgcgaa
      421 atcgctgcag gcgatggatt tcagcaccat cactgataaa tcctcataca acgccattga
      481 atggcccgcc tctccggaag gttcttcctg atttaattta tcgcgagaaa aacaatgtct
      541 gtagtgatat caggtgcgct gactgatggc gcaggtatcc ccatgtccgg ataccatatt
      601 attctgaaat cccgggtaaa caccccggaa gtggtgatga acactgttgc tgatgtgatg
      661 acaggaaacg atggtgaata ctgtttccat gcgcggactg gaaaatatgg tgtgtatctg
      721 aaacaggact ggcgcaacga gtacaacgtt ggcgacattg ctgtatatga ggactcaaag
      781 cccggcacgc tgaatgactt tctgattgct cctgatgagg gcgacctgaa accggatgtc
      841 gtcaaacgct ttgaggaaat ggtggcgcag gcgcagcaga gcgccggggc cgcagccgga
      901 aacgcacagc agacggcgca ggatgtggcg gcagccgcag gttatgcccg
//
LOCUS       DAJTRN010000288          937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRN010000288 DAJTRN010000000
VERSION     DAJTRN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(191..856)
                     /locus_tag="OO328_004208"
     CDS             complement(191..856)
                     /locus_tag="OO328_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5932520.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          242 a          231 c          211 g          253 t
ORIGIN      
        1 aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc ttgccggacg
       61 cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct gaagcagata
      121 gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat cagaaaggtg
      181 ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt ttgataagag
      241 ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca cagccacaat
      301 attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac gagccgccct
      361 gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg gctcgccgcc
      421 cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa ctgctgatca
      481 cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca
      541 tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt tcaaaccagt
      601 tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca aagcgaatat
      661 tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg tagaaaaaga
      721 tggtgtagtc gatttccgag gcctgaagcg cctccatcac ctcaccggaa catggagcac
      781 agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg ggccgtttga
      841 aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag agtgtagcat
      901 tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTRN010000289          934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRN010000289 DAJTRN010000000
VERSION     DAJTRN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..364)
                     /locus_tag="OO328_004209"
     CDS             complement(<1..364)
                     /locus_tag="OO328_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5932521.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(431..>934)
                     /locus_tag="OO328_004210"
     CDS             complement(431..>934)
                     /locus_tag="OO328_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HCR5932522.1"
                     /translation="EDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLI
                     GYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGV
                     QEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLPVARLE
                     DGTLLNW"
BASE COUNT          279 a          245 c          201 g          209 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aataattaag
      421 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatact
      481 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      541 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtacgggaaa acttcaagca
      601 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      661 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      721 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      781 tgatcgtcgt gcgaatatag ccaatcagct gctcttcgat taactgcttg cagtcccaga
      841 tactgttaaa cacttcaccg accgcaatcg gcgtcacggt atgctggcga atcagacgaa
      901 aacattcctg gttctcggca ggcgtcggat cttc
//
LOCUS       DAJTRN010000290          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRN010000290 DAJTRN010000000
VERSION     DAJTRN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(41..100)
                     /locus_tag="OO328_004211"
     CDS             complement(41..100)
                     /locus_tag="OO328_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5932523.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..>933)
                     /locus_tag="OO328_004212"
     CDS             complement(339..>933)
                     /locus_tag="OO328_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932524.1"
                     /translation="EAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRA
                     KAEKEAAEMQAAAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQA
                     LPAVIEKSVEPMKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRT
                     QAPLIDSLLNEIGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          194 a          249 c          226 g          264 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tct
//
LOCUS       DAJTRN010000291          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRN010000291 DAJTRN010000000
VERSION     DAJTRN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>933
                     /locus_tag="OO328_004213"
     CDS             <1..>933
                     /locus_tag="OO328_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932525.1"
                     /translation="SALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREA
                     AREIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFY
                     QDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGT
                     WTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSR
                     GFTKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSL
                     SNGSVKFSNVEPYMIERMKAELPDYNFSGDGKDWL"
BASE COUNT          238 a          198 c          267 g          230 t
ORIGIN      
        1 ctcggcgtta tccgtgccgt ttggtggcgg gaaaggcgct aagcaacagt ggattgagtt
       61 tgagtatcac aacctcgaca ggtttgagga aatattcatc tcgatggacg ttgatgatgt
      121 tggtcgtgaa gccgcaaggg aaatcgtaag ccgactcggt gaacatcgtt gccgtcttgt
      181 tactctgccg tacaaagaca tcaacgaatg cctgatgaac ggtgttaccg aggatgaaat
      241 ctggcagtac atcggcacgg catcctactt cgatcctgaa gaactctaca gtgcgcgaga
      301 gttttaccag gacactatca acgctttcta cggcaagcag cagtatctgt ttaatccacc
      361 gtgggaatct ctggcagata aattccagtt ccgtgaggca gagttgacgc tggtcaatgg
      421 tgtgcacggt cacggaaagg catgcccact gaatgagcct attcttttag ctgatgggac
      481 atggactact cacgggaatg taaaaattgg cgatcaggtg gcgtcagtag acggcaatcc
      541 gtcaactgtc actgggatat tcccgcaggg tgttagagat gtttaccgag tcacatttga
      601 agatggtcgt tatgttgatt gcgcaggcga tcacctatgg gaggtcacta gtcgtggatt
      661 cacgaaaggc gagaaacgcc gcgtgataga taccttcggg ttgaagcggt tgagtgaaac
      721 gaagaggcac aaaaatggcg ttaggattcc tgaaataact ggtgactttg gcgaccactc
      781 agagccatta gcatgggtta tcggctccct tctcggggat ggtagtctta gcaatgggag
      841 cgtgaagttt tcaaacgtcg agccatacat gatcgagcgt atgaaggctg aactgcctga
      901 ttacaacttc tctggagatg gtaaggactg gct
//
LOCUS       DAJTRN010000292          900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRN010000292 DAJTRN010000000
VERSION     DAJTRN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..900
                     /locus_tag="OO328_004214"
                     /pseudo
     CDS             1..900
                     /locus_tag="OO328_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          242 a          229 c          208 g          221 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
//
LOCUS       DAJTRN010000293          875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRN010000293 DAJTRN010000000
VERSION     DAJTRN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..88)
                     /locus_tag="OO328_004215"
     CDS             complement(<1..88)
                     /locus_tag="OO328_004215"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932526.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
     gene            300..866
                     /locus_tag="OO328_004216"
     CDS             300..866
                     /locus_tag="OO328_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5932527.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT          285 a          176 c          181 g          233 t
ORIGIN      
        1 ctctggataa ttgagatagt gtcttcccct tcatacgaac agcagccttt atctgctctg
       61 cgtgccagtc ttggtttttt gctgacattc tgcgatcctt actctcttaa cgttttcggt
      121 aactgatacc ggctaccgct atcgggaaaa acgatagcgc agaaaaatgc tcaattcaaa
      181 agctatgcgc atttttattt taaccagtgc aaatcaaaga attctgtaaa attctttata
      241 taacagagag attgggcatg acaggtgaaa aagaaagcaa aaatgcagag cgcattttta
      301 tgcccccgga aataatgcgt ttcaaggaac gccttgaaga ggcaatgaat ggagaatcaa
      361 gccgggcgtt tgcagctaag tgcggacttt ctgatggggt tatcagaaac tacctgtctg
      421 gcaagacata cccgtcttta gatcgattag cacaaatagc ctacgcaaca gggcgtccaa
      481 tagagtggtt tatccaaagt cagagcatgc caatggttga gcataaaaat aatcaagaga
      541 ataaaaattc tcaacctgac cgcagcacat tgaaaactaa actgatcgca attgtcgaat
      601 tgttagagga caaggaactg cattctgcga ttgagttgtt cagaaagaaa ggatttgaag
      661 ctcttatgcc tgagattttt tcttccgatg attccatcca ggctgatttg caaggtattt
      721 cgcagcaaac cttacaaaca gccaaaatgc ttgaatcatt accaacagat actcgtaaaa
      781 agattttgtc aaagtatggt atttatgaac aggaaggtct tgtagctcct tcacaagaac
      841 cacaagatgt aaaaaaagcc gtataacaac catga
//
LOCUS       DAJTRN010000294          867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRN010000294 DAJTRN010000000
VERSION     DAJTRN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..838
                     /locus_tag="OO328_004217"
                     /pseudo
     CDS             1..838
                     /locus_tag="OO328_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
BASE COUNT          192 a          219 c          262 g          194 t
ORIGIN      
        1 gaaaatcatc gggcagagtt cagcatcaaa gcgatgtgtc gtgtacttcg ggttgcccgc
       61 agcggctggt atgtctggct caggcgtcgt caccagatga gcctgcgcca acagtttcgg
      121 ctcacctgcg atgccgctgt ccataaggca ttctttgagg caaagcagcg atacggtgct
      181 ccccgccttg ctgacgaact gccggagttc aatattaaaa ccattgccgc cagcctgcgt
      241 cgtcaggggc tgcgggcgaa agccagccgg aagttcagcc cggtcagcta ccgtgcacat
      301 ggcctgcccg tattggagaa tctgctggag caggacttca gcgccagtgg cccgaaacca
      361 gaagtgggcg ggtgacatca cgtacttgcg taccgatgag ggctggttgt atctcgcagt
      421 agtcatcgac ctgtggtcac gcgccgtcat tggctggtcg atgtcaccgc gaatgacagc
      481 acaactggcc tgtgatgcac tgcaaatggc gttgtggcgg agaagacgcc cggaaagcgt
      541 cattgttcat acggaccgtg gtggtcaata ctgttcaggg gattatcagg caatgctgaa
      601 gcgacacaac ctgcgtggca gtatgagtgc gaaaggctac tgttatgaca atgcctgtgt
      661 ggaaagcttc tttcattcgc tgaaggtgga atgtatccac ggggaacgct ttagcagccg
      721 ggaaataatg cgggcaacgg tgtttaatta tatcgagtgt gattacaatc gctggcgtcg
      781 tcacagtgcc tgtggcggtc tcagcccgga acaatttgaa aaccataatc tcgcttaggg
      841 ccgtgtccac attacgtggg taggatc
//
LOCUS       DAJTRN010000295          854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRN010000295 DAJTRN010000000
VERSION     DAJTRN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            152..>854
                     /locus_tag="OO328_004218"
     CDS             152..>854
                     /locus_tag="OO328_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5932528.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT          280 a          163 c          159 g          252 t
ORIGIN      
        1 tgattcacat aatctctcat cattaatgag aacattgtca catatttaca atataaaact
       61 gttactttta gcaagctgtt agtaaccaac cgttactaat aattttaatt cttaatactt
      121 tgttcattca ataaatgagt tgagagaata tatgaataaa atatactccc ttaaatatag
      181 tgctgccact ggcggactca ttgctgtttc tgaattagcg aaaagagttt ctggtaaaac
      241 aaaccgaaaa cttgtagcaa caatgttgtc tctggctgtt gcaggtacag taaatgcagc
      301 aaatattgat atatcaaatg tatgggcgag agactatctt gatcttgcac aaaataaagg
      361 tattttccag cccggagcaa cagacgtaac aatcacttta aaaaacggag ataaattctc
      421 tttccataat ctctcaattc cggatttttc tggtgcagca gcgagtggcg cagctaccgc
      481 aataggaggt tcttatagtg ttactgttgc acataacaaa aagaaccctc aggccgcaga
      541 aactcaggtt tacgctcagt cttcttacaa ggttgttgac agaagaaatt ccaatgattt
      601 tgagattcag aggttaaata aatttgttgt ggaaacagta ggtgccaccc cggcagagac
      661 caaccctaca acatattctg atgcattaga acgctacggt atagtcactt ctgacggttc
      721 aaaaaaaatc ataggttttc gtgctggctc tggaggaaca tcatttatta atggtgaatc
      781 caaaatctca acaaattcag catatagcca tgacctgtta agtgctagtc tatttgaggt
      841 cacccaatgg gact
//
LOCUS       DAJTRN010000296          837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRN010000296 DAJTRN010000000
VERSION     DAJTRN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..605
                     /locus_tag="OO328_004219"
     CDS             <1..605
                     /locus_tag="OO328_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5932529.1"
                     /translation="VNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQ
                     EIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSP
                     LDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASA
                     SLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(607..837)
                     /locus_tag="OO328_004220"
                     /pseudo
     CDS             complement(607..837)
                     /locus_tag="OO328_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          220 a          206 c          179 g          232 t
ORIGIN      
        1 tggtgaataa acccgcgcca tccatgccag taatccatga cgttccaaaa aatcatcgtg
       61 atgacttacc tattgcaatt ctggctgccg gaatggttaa tgaaaatgat ctgatcttct
      121 ttgataatgg ccaggagata ccactcgtta taagcatgat cccggatgca atcaccttca
      181 ccggcatctg ttactcacat cgcgtctttg ttgcgttgaa tgaaaagcct aatgtaacag
      241 caatactttg tggtggtacg tatcgtgcca gaagtgatgc tttttacgat gccagtaact
      301 cttcgccatt agactctctc aatccgcgaa aaatatttat ttccgccagc ggtgtgcata
      361 atcactttgg cgtcagctgg tttaaccctg aagatcttgc cactaagcgt aaagtgatga
      421 accgtggact acggaaaatt ttgctcgccc gccacgcgtt gttcgatgaa gtggcctctg
      481 ccagcctcgc accgatctct gcatttgacg ttctgattag cgatcgtccg ttaccggcag
      541 attatgttac gcactgccag aatggttctg taaagatcat tacacctgat tcagaagacg
      601 aatgacttac tgaaaaaaca ccacaatctt gttaaacatc gtcgggttgg actgattacg
      661 ttgcactttc accacatatt ccagcttatc tatttggctt atcacctact ccagacgctg
      721 gtcatccttg accagtagcc agatatggct tttgtcgcag tcctgaatcg gcaggcagag
      781 aatgtcttca acgttaagag cgcgacgggc aaaagcccac aaaggtgagt cattaca
//
LOCUS       DAJTRN010000297          820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRN010000297 DAJTRN010000000
VERSION     DAJTRN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            389..>820
                     /locus_tag="OO328_004221"
     CDS             389..>820
                     /locus_tag="OO328_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HCR5932530.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDIN"
BASE COUNT          214 a          164 c          207 g          235 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgttg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac
//
LOCUS       DAJTRN010000298          792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRN010000298 DAJTRN010000000
VERSION     DAJTRN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            196..447
                     /locus_tag="OO328_004222"
     CDS             196..447
                     /locus_tag="OO328_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932531.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            514..792
                     /locus_tag="OO328_004223"
                     /pseudo
     CDS             514..792
                     /locus_tag="OO328_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          211 a          189 c          190 g          202 t
ORIGIN      
        1 cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc cgcttcggcg
       61 gttttttgtt tttattttca acgcatttga agttctggat ggtgccggaa tagaatcaaa
      121 aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga gtgctactta
      181 tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg tgaagatggc
      241 ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat tgtgtgtttg
      301 caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga cggatttatc
      361 ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg aataaaagct
      421 ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg gctgaactcc
      481 cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa atattacaat
      541 catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc atttaagtca
      601 gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat tcagtgttac
      661 cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca ctagataatc
      721 tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt ccagcaacgg
      781 atctggaggt aa
//
LOCUS       DAJTRN010000299          792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRN010000299 DAJTRN010000000
VERSION     DAJTRN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            71..694
                     /gene="tetR(B)"
                     /locus_tag="OO328_004224"
     CDS             71..694
                     /gene="tetR(B)"
                     /locus_tag="OO328_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5932532.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(672..>792)
                     /locus_tag="OO328_004225"
     CDS             complement(672..>792)
                     /locus_tag="OO328_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5932533.1"
                     /translation="GAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
BASE COUNT          264 a          145 c          169 g          214 t
ORIGIN      
        1 ctatcactga tagggagtgg taaaataact ctatcaatga tagagtgtca acaaaaatta
       61 ggaattaatg atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct
      121 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
      181 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
      241 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
      301 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
      361 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
      421 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
      481 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
      541 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
      601 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
      661 agaaaaacaa cttaaatgtg aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg
      721 gtaacttcac ggtaaccaag atgtcgagtt aaccaccctt tagattcata aagcgaaaat
      781 aatgcggctc ca
//
LOCUS       DAJTRN010000300          790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRN010000300 DAJTRN010000000
VERSION     DAJTRN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..480
                     /locus_tag="OO328_004226"
     CDS             <1..480
                     /locus_tag="OO328_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5932534.1"
                     /translation="WNIDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPV
                     MDDPCPFNQAPFYNGKSDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFG
                     KKRRAYVLNNGGLRMSYVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            467..>790
                     /locus_tag="OO328_004227"
     CDS             467..>790
                     /locus_tag="OO328_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5932535.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATT"
BASE COUNT          188 a          183 c          226 g          193 t
ORIGIN      
        1 tggaacatcg ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc
       61 agtcgctggc tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg
      121 gacaccccgg taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa
      181 tcggataccc ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa
      241 gccatgtcga acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg
      301 ttcggaaaaa aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc
      361 tttgagtccg cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca
      421 tccccgccgg gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga
      481 taaaccccgc cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga
      541 caaggcgaca cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc
      601 ggtgaccatg acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg
      661 tctgatgtac gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta
      721 taatgccggt tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt
      781 cgcaacaaca
//
LOCUS       DAJTRN010000301          757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRN010000301 DAJTRN010000000
VERSION     DAJTRN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(67..>757)
                     /gene="arsB"
                     /locus_tag="OO328_004228"
     CDS             complement(67..>757)
                     /gene="arsB"
                     /locus_tag="OO328_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5932536.1"
                     /translation="DIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFVLEPL
                     GIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLT
                     EYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEA
                     MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTL
                     AALALRLSFTL"
BASE COUNT          208 a          203 c          195 g          151 t
ORIGIN      
        1 cgtattacgc gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt
       61 atctcattac aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac
      121 aggcagggtc atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt
      181 ctgcgaaagt acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg
      241 ccccaaatcg cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc
      301 cgtgctgcca tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga
      361 agagaggaag gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc
      421 cagcacgttg agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac
      481 caccagatac atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt
      541 acccgtatta atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc
      601 tgcaatggcg ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag
      661 cagtaaaaca acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga
      721 tttcagcagc gccatatcgt agttctgcgg aatatct
//
LOCUS       DAJTRN010000302          755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRN010000302 DAJTRN010000000
VERSION     DAJTRN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..337
                     /locus_tag="OO328_004229"
     CDS             <1..337
                     /locus_tag="OO328_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5932537.1"
                     /translation="VLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGI
                     DTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVR
                     SVEEILHAL"
     gene            692..>755
                     /locus_tag="OO328_004230"
     CDS             692..>755
                     /locus_tag="OO328_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein DinI"
                     /protein_id="HCR5932538.1"
                     /translation="MRFEICIAKEKMTKMPTGAVD"
BASE COUNT          218 a          177 c          169 g          191 t
ORIGIN      
        1 agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg acagtgatat
       61 cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt tgcaactgcg
      121 ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg gtgttgaatc
      181 caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag acgcctgtag
      241 tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc gcatcgcccg
      301 tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct accgcaatgg
      361 tcacatccta aatgggtgcg gttggggatc accagccttg aagagtatgc ccgccacttt
      421 aactgcgtga tacgggattt ttaaaaatca ctaaagaacg cccaagagca tgtgttttct
      481 ttagaataat caaagcgttg gattgccatg tcatatgaaa atcggcaaag ttcatatgta
      541 acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc cccatgcatg
      601 ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt aaaaataata
      661 ctgtataaaa acacagtgca gagggatttt tatgcgtttt gaaatctgca tagccaaaga
      721 gaaaatgact aaaatgccaa ccggtgctgt ggatg
//
LOCUS       DAJTRN010000303          752 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRN010000303 DAJTRN010000000
VERSION     DAJTRN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>752
                     /locus_tag="OO328_004231"
     CDS             <1..>752
                     /locus_tag="OO328_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5932539.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKAL"
BASE COUNT          208 a          153 c          182 g          209 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      721 atccatcggt tactcctatt ctaaagcact gg
//
LOCUS       DAJTRN010000304          746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRN010000304 DAJTRN010000000
VERSION     DAJTRN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>746
                     /locus_tag="OO328_004232"
     CDS             <1..>746
                     /locus_tag="OO328_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="catalyzes the reduction of trimethylamine-N-oxide
                     to form trimethylamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR5932540.1"
                     /translation="AFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVE
                     FDHPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSH
                     GGPGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNG
                     DVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTID
                     IGTSQLAQATSAHTTLVEIEKYNGTVEQVT"
BASE COUNT          178 a          188 c          229 g          151 t
ORIGIN      
        1 agcctttacc gaagggctgg acgaaatggg ctggctgaaa cgcatctggc aggaaggtgt
       61 acaacaaggc aaaggacgcg gcgttcatct gccagcgttt gatgacttct ggaataacaa
      121 agagtacgtc gagtttgacc atccgcagat gtttgttcgc caccaggcat tccgcgaaga
      181 tccggatctc gaaccgctgg gcacgccgag tggcctgatt gagatctact cgaaaaccat
      241 cgccgatatg aactacgacg attgtcaggg gcatccgatg tggtttgaga aaatcgaacg
      301 ctcccacggt gggcccggct cgcagaagta tccgttgcat ctgcaatctg tgcatccgga
      361 tttccgactt cactcgcagc tatgtgagtc ggaaacgctg cgtcagcaat atacggtagc
      421 gggtaaagag ccagtgttca ttaacccgca ggatgccagc gcgcgcggta ttcgtaacgg
      481 tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg gcaggagcag tagtttctga
      541 ccgctatgca cccggcgtgg cgcgaattca cgaaggggca tggtacgatc cagataaagg
      601 cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac gtgctgacca tcgacatcgg
      661 gacttcgcag ctggcgcagg cgaccagtgc gcacactacg ctggtggaaa ttgagaagta
      721 caatggaaca gtggagcagg tgacgg
//
LOCUS       DAJTRN010000305          743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRN010000305 DAJTRN010000000
VERSION     DAJTRN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO328_004233"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO328_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5932541.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          273 a           95 c          145 g          230 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattag taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa aaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca aca
//
LOCUS       DAJTRN010000306          732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRN010000306 DAJTRN010000000
VERSION     DAJTRN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..597
                     /gene="nanR"
                     /locus_tag="OO328_004234"
     CDS             <1..597
                     /gene="nanR"
                     /locus_tag="OO328_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5932542.1"
                     /translation="NVGRPSVREALAALKRKGLVQINNGERARVSRPSADTIIGELSG
                     MAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSLDNNAA
                     FIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQQHIAI
                     VDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT          182 a          191 c          174 g          185 t
ORIGIN      
        1 aacgtcgggc gtccttctgt gcgtgaagcg ctggcagcgt taaaacgcaa aggtctggtg
       61 caaataaaca acggcgaacg cgctcgtgtc tctcgacctt ctgcggacac tatcatcggc
      121 gagctttccg gcatggcgaa agatttcctt tctcatcctg gtgggattgc ccatttcgaa
      181 caattacgtc tgttctttga atccagcctg gtgcgctatg cggctgaaca cgccaccgat
      241 gagcaaatcg atttgctggc aaaagcactg gaaatcaaca gtcagtcgct ggataacaac
      301 gcggcattca ttcgttcaga cgttgatttc caccgcgtgc tggcggagat ccccggtaac
      361 ccaatcttca tggcgatcca cgttgccctg ctcgactggc ttattgccgc acgcccaacg
      421 gttaccgatc aggcactgca cgaacataac aacgttagtt atcaacagca tattgcgatc
      481 gttgatgcga tccgccgtca tgatcctgac gaagccgatc gtgcgttgca atcgcatctc
      541 aacagcgtct ctgctacctg gcacgctttc ggtcagacca ccaacaaaaa gaaataatgc
      601 cactttagtg aagcagatcg cattataagc tttctgtatg gggtgttgct taattgatct
      661 ggtataacag gtataaaggt atatcgttta tcagacaagc atcacttcag aggtatttat
      721 ggcaacgaat tt
//
LOCUS       DAJTRN010000307          721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRN010000307 DAJTRN010000000
VERSION     DAJTRN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     misc_feature    complement(51..174)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(150..191)
                     /gene="hisL"
                     /locus_tag="OO328_004235"
     CDS             complement(150..191)
                     /gene="hisL"
                     /locus_tag="OO328_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5932543.1"
                     /translation="MTRVQFKHHHHPD"
     gene            474..>721
                     /gene="yefM"
                     /locus_tag="OO328_004236"
     CDS             474..>721
                     /gene="yefM"
                     /locus_tag="OO328_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5932544.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
BASE COUNT          217 a          148 c          157 g          199 t
ORIGIN      
        1 tcattttctt attcctcttt aaacctgtct gaaccgttaa caaccgcgca ataaaaaagc
       61 ccccggaagg tgatcttccg ggggctttct catgcgttca tgcaccactg gaagatctga
      121 atgtcttcca gcacacatcg cctgaaagac tagtcaggat gatggtggtg tttaaattga
      181 acgcgtgtca taaaaatctc tgtgaatgtt tattcaactg atgtctttta acctaaacca
      241 ctttcacgtt agaaagcaag ggctttttta tatattttat ggcgatggat ttgtttaatg
      301 aatgagcggg gaaaggaggg ggctaagagg actgtcctct aacttgacag caccaaaaag
      361 agcaaagtct gatttttcgt ctcgctggca atgtcttgat ctacaaacta attaataaat
      421 agttaattaa cacctatcat tgtacaatga actgtacaaa ataggagatt tacatgcgta
      481 caattagcta cagcgaagcg cgtcagaatt tgtcggcaac aatgatgaaa gccgttgaag
      541 atcatgcccc gatcctcatt actcgtcaga atggagaggc ttgtgttctg atgtcactcg
      601 aagaatacaa ttcgctggaa gagacggctt atctactgcg ttcccccgct aacgcccgga
      661 gattgatgga ctcaatcgat agcctgaaat caggcaaagg aacggaaaag gacattattg
      721 a
//
LOCUS       DAJTRN010000308          720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRN010000308 DAJTRN010000000
VERSION     DAJTRN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>720
                     /locus_tag="OO328_004237"
     CDS             <1..>720
                     /locus_tag="OO328_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5932545.1"
                     /translation="SYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHG
                     IASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQ
                     EKITKDKDIVFTNGGNVLFKDNLDFGCGGIIFDEGHEYNINGQRFTFKGAGIDIGKES
                     IVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGK
                     VILNKDNSLGNDQYAGIFFTKR"
BASE COUNT          285 a           98 c          143 g          194 t
ORIGIN      
        1 tcatacggca tgatgattta taaaaatgat aaaacatttc gtaatcttga aatattcgga
       61 gacagcggct ctggagcata cttatatgat aacaaactag aaaaatgggt attagtcgga
      121 acaacccatg gtattgccag cgttaatggt gaccaactga catggataac aaaatacaat
      181 gataaactgg ttagtgagtt aaaagatacc tatagtcata aaataaatct gaatggcaat
      241 aatgtaacca ttaaaaacac agatataaca ttacaccaaa acaatgcaga taccactggt
      301 actcaagaaa aaataactaa agacaaagat attgtgttca caaatggggg aaatgttctg
      361 tttaaggata atttggattt tggttgcggt ggtattatct ttgacgaagg ccatgaatat
      421 aacataaacg gtcagagatt tacatttaaa ggagcaggaa ttgatatcgg aaaagaaagc
      481 attgtaaact ggaatgcatt gtattccagt gatgatgttt tacacaaaat aggccccggt
      541 actctgaatg ttcaaaaaaa acagggggca aatataaaga taggtgaagg aaatgttatt
      601 cttaatgaag aaggaacatt taacaatata taccttgcaa gcggaaatgg taaggtaata
      661 ctaaataaag ataattccct tggcaatgat caatatgcgg ggatattttt tactaaacgt
//
LOCUS       DAJTRN010000309          709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRN010000309 DAJTRN010000000
VERSION     DAJTRN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>709
                     /locus_tag="OO328_004238"
     CDS             <1..>709
                     /locus_tag="OO328_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5932546.1"
                     /translation="VIQFQYRYRWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSG
                     TDPRTYFEEKVENSMTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPA
                     SSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGS
                     RAEVGDRVLSYHELAKIWLAIERSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLL
                     DNVWTVPKEHSKMGNVIR"
BASE COUNT          186 a          172 c          185 g          166 t
ORIGIN      
        1 gggtcatcca gttccagtac cgctatcgct ggcatggcaa gcctaatcga cttgggcttg
       61 gtcgataccc atccctgtct ttgaaggatg ccagacagat cactgctgac ttgcgaaagc
      121 tctatttctc aggaacggat ccacgcacct attttgaaga gaaggtggag aactccatga
      181 cggtcgccca gtgtctcgac tactggttcg acaactacgt ctctacaact ctcagagaaa
      241 agacccaggc actttatcga tcaacggtta tgaagcgcat gcatgacgcc ttccctaatc
      301 gtccggcatc ttctatcacg gttaagcaat gggttgacct gcttaccgaa gaagaaaaag
      361 ataatccacg ccgagcaagg caggtgctaa gtcaactaag atcagcaatt agttggtgca
      421 tgcggcgtca gttgatagat agttgcgcaa ttatgagcat ccagccaagg gacttcggct
      481 cccgcgctga ggtaggggat cgggtactgt cgtatcacga actggctaag atttggcttg
      541 ctattgaaag aagccgtgcg tctacgtcaa ataagctcct tcatcagatg cttatgctgt
      601 ggggggcgag gctctcagag cttaggctgg caacaaagac agaatttgac ctgctggaca
      661 acgtatggac cgtaccgaaa gagcatagca agatgggtaa tgttatccg
//
LOCUS       DAJTRN010000310          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRN010000310 DAJTRN010000000
VERSION     DAJTRN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>702
                     /gene="lsrF"
                     /locus_tag="OO328_004239"
     CDS             <1..>702
                     /gene="lsrF"
                     /locus_tag="OO328_004239"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5932547.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMM"
BASE COUNT          166 a          147 c          210 g          179 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat ga
//
LOCUS       DAJTRN010000311          700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTRN010000311 DAJTRN010000000
VERSION     DAJTRN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            459..>700
                     /locus_tag="OO328_004240"
     CDS             459..>700
                     /locus_tag="OO328_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5932548.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          236 a          135 c          114 g          215 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa
//
LOCUS       DAJTRN010000312          696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRN010000312 DAJTRN010000000
VERSION     DAJTRN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            103..>696
                     /locus_tag="OO328_004241"
     CDS             103..>696
                     /locus_tag="OO328_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5932549.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLAL"
BASE COUNT          242 a          134 c          119 g          201 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctg
//
LOCUS       DAJTRN010000313          687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRN010000313 DAJTRN010000000
VERSION     DAJTRN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..651
                     /locus_tag="OO328_004242"
     rRNA            <1..651
                     /locus_tag="OO328_004242"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          163 c          200 g          149 t
ORIGIN      
        1 acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg gtggagcatg
       61 tggtttaatt cgatgcaacg cgaagaacct tacctggtct tgacatccac ggaagttttc
      121 agagatgaga atgtgccttc gggaaccgtg agacaggtgc tgcatggctg tcgtcagctc
      181 gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa cccttatcct ttgttgccag
      241 cggtccggcc gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga
      301 cgtcaagtca tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa
      361 agagaagcga cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga
      421 gtctgcaact cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg
      481 tgaatacgtt cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa
      541 gaagtaggta gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg
      601 aagtcgtaac aaggtaaccg taggggaacc tgcggttgga tcacctcctt accttaaaga
      661 agcgttcttt gtagtgctca cacagat
//
LOCUS       DAJTRN010000314          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRN010000314 DAJTRN010000000
VERSION     DAJTRN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..90)
                     /locus_tag="OO328_004243"
     CDS             complement(<1..90)
                     /locus_tag="OO328_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5932550.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMF"
     gene            255..>683
                     /gene="adeP"
                     /locus_tag="OO328_004244"
     CDS             255..>683
                     /gene="adeP"
                     /locus_tag="OO328_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5932551.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVS"
BASE COUNT          151 a          166 c          166 g          200 t
ORIGIN      
        1 aaacatcgtt acatatgcgc gggagcaaat gacttcactg tcgaccagcg taccgtcgca
       61 gtcgaaaaat accgcttcta tccgggacat gcctttcctt tttaatcagt ttaacgtttt
      121 cgtagagccg atttgcgcaa cgcaatcgtt gccgtataag taaatccaat aaaaaagtct
      181 ctcacaaacg cgcctattgt cgcattttgg tatacgatag cgacggattt tccctccttg
      241 ttcggaaata gataatgagt catcaacata ccacccagac ttctggtcag aggatgctgg
      301 aacgtgtttt taaactgcgc gaacatggta ctacggcacg aactgaagtg attgccgggt
      361 tcaccacctt cctgacgatg gtctacatcg tatttgttaa cccacaaatt ctgggcgttg
      421 ctggcatgga caccagtgcc gtcttcgtaa caacctgtct gattgctgct ttcggtagca
      481 ttatgatggg attgtttgca aaccttccgg tggcgctggc tcctgcgatg gggcttaacg
      541 cctttttcgc ttttgtggtt gtccaggcaa tggggctgcc gtggcagatc ggtatgggcg
      601 caatcttctg gggcgctgtc ggactgcttc ttttgaccat tttccgcgtt cgttactgga
      661 tgattgctaa cattccggta agt
//
LOCUS       DAJTRN010000315          681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRN010000315 DAJTRN010000000
VERSION     DAJTRN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..276)
                     /locus_tag="OO328_004245"
     CDS             complement(<1..276)
                     /locus_tag="OO328_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932552.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLE"
     gene            complement(273..656)
                     /locus_tag="OO328_004246"
     CDS             complement(273..656)
                     /locus_tag="OO328_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932553.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          147 a          196 c          176 g          162 t
ORIGIN      
        1 ctccagcacg accggtaatg gcgataaaaa catcgccata tcctcaatgt aagggtggga
       61 cttctccgga ttcagcacca cacagtccgc cttctgttgc tcactcaggg catgtaaatc
      121 atgctcaaac cacgccccct gagcatctgt ctgcagaatc aaccggccat ggcaggaaag
      181 gcagaaacac tgccggatat ttctgctgag cgcagagtcg gcactgataa tgtgctcacc
      241 cggcgtgatc cccagccccg tttttatacc gttcattctg ctcccccctc ctcactgaag
      301 cgcccggtct ggcagtgtgt gcagtactgc tccccataat aatcgtggtg acattgcctg
      361 cagtgccagc tggctttacg tactacgggt aaggtatccg ggacgaattg ctgcagacgt
      421 ttaatcaacc gtatttctct gcgttccggc ctgacataag ggcactcatg accgtgctcc
      481 gtcagccctt cgtcagcgtg ttcaaaccag ggccgttcag tgtcgtattg cgggtggtat
      541 ctgagtgcac tgccgcaaag gtggcaggtg tagctatcgt aaggtgcagt ctgtgcggta
      601 cgggcaccgg tcagacgtcc gttgccatca agtgcgagaa aagattttgc gtacatagta
      661 tatgttcctc agccgccaga c
//
LOCUS       DAJTRN010000316          680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRN010000316 DAJTRN010000000
VERSION     DAJTRN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(125..255)
                     /locus_tag="OO328_004247"
                     /pseudo
     CDS             complement(125..255)
                     /locus_tag="OO328_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(253..348)
                     /locus_tag="OO328_004248"
                     /pseudo
     CDS             complement(253..348)
                     /locus_tag="OO328_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          195 a          123 c          163 g          199 t
ORIGIN      
        1 tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt tattttttta
       61 cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg ggtggcgctt
      121 ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt ggatttccac
      181 ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc ggagggtcag
      241 gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg tcacctgttt
      301 atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc cacacaatta
      361 aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc ctcgcagctt
      421 tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg agactccaca
      481 aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga ttcacttcat
      541 caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg acgatttcct
      601 ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct attgagcttt
      661 atttcaaata tggtaagaag
//
LOCUS       DAJTRN010000317          679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRN010000317 DAJTRN010000000
VERSION     DAJTRN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(27..215)
                     /locus_tag="OO328_004249"
     CDS             complement(27..215)
                     /locus_tag="OO328_004249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932554.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          167 a          134 c          166 g          212 t
ORIGIN      
        1 tgaatcggag aacaaactgt gagctatcag cctggtggtc tggttttgcc tgatttaagc
       61 cgccgtgggt atttgtccaa gaatgcttca agctccaatc gttttggcct ggtgaatgga
      121 gatgggttaa gcaaagactc tcgttgctgg tgtacttttt cgcgctggcg ctcaagcatg
      181 gataatgcga ttgcctgtag ttcttcgtta gtcattgtct gcacctggaa agcaatttac
      241 cggagtacct cacactacgg caaaaatgtt ttattgttgc aacttgcgta attattcagg
      301 tcagtttgga acgcactgta taaacataaa taacctgata ctgttgtgtg ttttcaaaga
      361 aaacacaggt atttttcaca taaaccttcc ccttttcata ttcgaccact taatcaaaaa
      421 acggttttat acttgttgcc cccactgggg ttaaatgtga gacatgtcgc actttgtgcc
      481 atgtgtcctc gctgatttta ggcgcggttc attcattctt tctgttggca taaagtatgg
      541 tatcggttgg gcgaatgaaa gggactcggg tcatggtcag acaatacctg aggcgttaat
      601 tcgttgaaag agattgtgaa tgggggaata actgagtctt aactgctgtg cgatagcccg
      661 gacagatagt tgctcgtcg
//
LOCUS       DAJTRN010000318          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRN010000318 DAJTRN010000000
VERSION     DAJTRN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>668
                     /locus_tag="OO328_004250"
     CDS             <1..>668
                     /locus_tag="OO328_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5932555.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJTRN010000319          662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRN010000319 DAJTRN010000000
VERSION     DAJTRN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            148..603
                     /gene="csgB"
                     /locus_tag="OO328_004251"
     CDS             148..603
                     /gene="csgB"
                     /locus_tag="OO328_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5932556.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          216 a          115 c          153 g          178 t
ORIGIN      
        1 aaatacaacg cgcgggtgag ttattaaaaa tatttccgca gacatacttt ccatcgtaac
       61 gcagcgttaa caaaatacag gttgcgttaa caaccaagtt gaaatgattt aatttcttaa
      121 atgtacgacc aggtccaggg tgacaacatg aaaaacaaat tgttatttat gatgttaaca
      181 atactgggtg cgcctgggat tgcagccgca gcaggttatg atttagctaa ttcagaatat
      241 aacttcgcgg taaatgaatt gagtaagtct tcatttaatc aggcagccat aattggtcag
      301 gctgggacta ataatagtgc tcagttacgg cagggaggct caaaactttt ggcggttgtt
      361 gcgcaagaag gtagtagcaa tcgggcaaag attgaccaga caggagatta taaccttgca
      421 tatattgatc aggcgggcag tgccaacgat gccagtattt cgcaaggtgc ttatggtaat
      481 actgcgatga ttatccagaa aggttctggt aataaagcaa atattacaca gtatggtact
      541 caaaaaacgg cagttgtagt gcagagacag tcgcaaatgg caattcgcgt gacacaacgt
      601 taattaccat tcgactttta aatcaatccg atgggggttt tacatgaaac ttttaaaagt
      661 ag
//
LOCUS       DAJTRN010000320          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRN010000320 DAJTRN010000000
VERSION     DAJTRN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..249
                     /locus_tag="OO328_004252"
     CDS             <1..249
                     /locus_tag="OO328_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IID with IIC forms the translocation channel;
                     involved in N-acetylgalactosamine transport; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIA"
                     /protein_id="HCR5932557.1"
                     /translation="FLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVLEREG
                     LSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            246..>659
                     /locus_tag="OO328_004253"
     CDS             246..>659
                     /locus_tag="OO328_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5932558.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT          146 a          166 c          214 g          133 t
ORIGIN      
        1 tttctgacgg atttgctggg cggcacgccg tttcgcgtag cttcaacgct ggcgatgcaa
       61 aaaccgggct gtgaagtgat taccggcacc aatttgcaac tgctactgga gatggtgctg
      121 gagcgcgaag ggttaagcgg tgaagaattc cgtgtacagg cgctggagtg tggacaccga
      181 gggctgacca gtctggtgga cgagttaggc cgctgccatg aagaatgtcc ggtcgaggaa
      241 ggaatatgac acacgttctg cgcgccagaa ggctgctgac tgaagaggga tggttcgatg
      301 accatcagtt gcgtgttgct gacggtgtca tcgcagcaat cgaaccgatt ccagtgggcg
      361 tgactgaacg cgatgcggaa cggctctgcc ccgcttacat cgacacccat gtacacggtg
      421 gtgcgggcgt tgatgtaatg gatgacgcgc cggatgtact cgacaagctg gcaatgcaca
      481 aggcacgcga aggtgtcggc agttggttac cgaccaccgt aaccgcgccg cttaatacca
      541 ttcatgcggc gctgaaacgt attgctcaac gttgccaacg cggcggacct ggtgcgcaag
      601 tgctggggag ttatctcgaa ggaccgtact tcacgccgca gaataaaggc gcgcatccg
//
LOCUS       DAJTRN010000321          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRN010000321 DAJTRN010000000
VERSION     DAJTRN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            24..657
                     /locus_tag="OO328_004254"
                     /pseudo
     CDS             24..657
                     /locus_tag="OO328_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          197 a          164 c          166 g          130 t
ORIGIN      
        1 ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg
       61 aatccgctca actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg
      121 atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca
      181 aaacaccaaa agcctccccc attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      241 agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      301 cagactccct gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac
      361 actctgccat gtgttcgggg ttcatcgcag cagctacaga tactggaaaa accgtcctga
      421 aaaaccagac ggcagacggg ctgtattacg cagtcaggta cttgagctac atggcatcag
      481 ccacggttcg gccggagcaa gaagcatcgc cacaatggca acccggagag gctaccagat
      541 gggacgctgg cttgctggca ggctcatgaa agagctgggg ctggtcagct gtcagcagcc
      601 gactcaccgg tataaacgtg gtggtcatga acatgttgct atccctaact accttga
//
LOCUS       DAJTRN010000322          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRN010000322 DAJTRN010000000
VERSION     DAJTRN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          207 a          156 c          127 g          165 t
ORIGIN      
        1 gctatcggaa agaaaaaagg ccatcagcaa aactctgatg acctgaatca cctttaccaa
       61 tattgtatga aaaaacacgc acgactaatt gacaataatt ttcatttcca ttaaaaaaga
      121 tcgcgtagca ttcttaattc atgaggaact ttacccgcca gcaatctgag tagcgaaagc
      181 tgttcatccc caatgttttt gctggcgggt ccttttttct acggtcctcc tccccggagg
      241 ggggcatttc attatttttc taatcgttac tggtgagaag gcggaagaaa aagccagttc
      301 ttcgccccta aataacagca tgattccagt ccatgcaaaa tctgatttcc agctcacaca
      361 taccgagcgc tgtacaaaaa accgccagcc aaggcagtta agagtgtgtt cccggggttt
      421 gcttagaata ttttgtaagt tgtccgaaga gatatttaca acaccagaat gatgcattat
      481 cagtcccctg ccagaggttc actacgtaac ctgaccgaca atgcaccatt ctggtgctga
      541 aaaaagagca ctggaactgc aacaaaacaa catcacagaa caggagatgg taaggagtca
      601 gacattccca cacaatattg tgtcagtgca aataacaacc ttcgtctcaa atctc
//
LOCUS       DAJTRN010000323          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRN010000323 DAJTRN010000000
VERSION     DAJTRN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            389..>648
                     /locus_tag="OO328_004255"
     CDS             389..>648
                     /locus_tag="OO328_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5932559.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLP"
BASE COUNT          232 a          117 c          106 g          193 t
ORIGIN      
        1 gttaatttca gactcgttta agctccattt tcaaaaaaaa tatatcaggg tttgttacta
       61 ataacttctg aaaacagaaa aaaatagagc agtaattcta tttcagagag gagtaactcc
      121 atctatcctc gatacgtact gaacgttatc gtgatatatt catcatgctt tcttcaccat
      181 tattcgagta tagggagaga cgcaatgacc ttaaatacct ttttatggct gaattaccat
      241 tctcaggaat aacagcagac tccttcccat agacggctta gggggaaagc aatacccgga
      301 gaaagggtac aaagcaaact gttttaaaat taataagcca tattctattt aactagaatt
      361 cttatcgttg ataagaattc tagttataat gattaaatca accaatatac aggcaatcgg
      421 ttctggcatt atgcatcaaa taaacaatgt atactcgtta actccattat ctttacctat
      481 ggaactgact ccatcttgta atgaatttta tttaaaaacc tggagcgaat gggaaaagaa
      541 cggtacccca ggcgagcaac gcaatatcgc cttcaatagg ctgaaaatat gtttacaaaa
      601 tcaagaggca gaattaaatt tatctgagtt agatttaaaa acattacc
//
LOCUS       DAJTRN010000324          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRN010000324 DAJTRN010000000
VERSION     DAJTRN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            490..>648
                     /locus_tag="OO328_004256"
     CDS             490..>648
                     /locus_tag="OO328_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932560.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLD"
BASE COUNT          237 a          115 c          129 g          167 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 ggggggggga tgaaaatacc tgaagcagta aatcatatta atgttcagaa taatattgat
      541 cttgttgatg gaaaaacgaa tccgaataaa gcaacaaaag cattacaaaa aaatgtatta
      601 cgcgtaacaa actcatcttc ttctggcata agtgaaaaac atttagat
//
LOCUS       DAJTRN010000325          638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTRN010000325 DAJTRN010000000
VERSION     DAJTRN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>638
                     /locus_tag="OO328_004257"
     CDS             <1..>638
                     /locus_tag="OO328_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5932561.1"
                     /translation="TGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQN
                     SMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGN
                     IYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIW
                     HLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          203 a          136 c          137 g          162 t
ORIGIN      
        1 tacaggtggc atcactgcac ataacagcca aatagccata ggtgatcaag ctgtagttac
       61 acttaatggt gcaacctttc tgaataatac tcctataagt atagataaag gagcaaaagt
      121 tatagcacaa aattccatgt tcacaacaaa aggtattgat atctccggtg aactgactat
      181 gatgggaatc cctgaacaga atagtaaaac tgtaacgccg ggtctccact acgctgctga
      241 tggattcagg ctgagtggtg gaaatgcaaa tttcattgcc agaaatatgg catctgtcac
      301 cggaaatatt tatgctgatg atgcagcaac cattactctg ggacagcctg aaactgaaac
      361 accgactata tcgtctgctt atcaggcatg ggcagagact cttttgtatg gctttgatac
      421 cgcctatcga ggcgcaataa cagcccccaa agctacagtt agcatgaata atgcgatctg
      481 gcatctaaat agccagtcat caattaatcg tctagaaaca aaagacagta tggtgcgttt
      541 tactggtgac aatgggaagt ttacaaccct tacagtggac aaccttacta tagatgacag
      601 tgcatttgtg ctgcgtgcaa atctggccca agcagatc
//
LOCUS       DAJTRN010000326          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTRN010000326 DAJTRN010000000
VERSION     DAJTRN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            18..491
                     /locus_tag="OO328_004258"
     CDS             18..491
                     /locus_tag="OO328_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5932562.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTRN010000327          622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRN010000327 DAJTRN010000000
VERSION     DAJTRN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          165 a          122 c          149 g          186 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tc
//
LOCUS       DAJTRN010000328          616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRN010000328 DAJTRN010000000
VERSION     DAJTRN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            86..583
                     /locus_tag="OO328_004259"
                     /pseudo
     CDS             86..583
                     /locus_tag="OO328_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          149 a          153 c          190 g          124 t
ORIGIN      
        1 tctccaggaa acccggggcg gttcaaagcg ccagccccgt cccgcccgat agagcgcggc
       61 atagcgggcc ccggattact tgcgtgcgac ggcagaagaa gccctgaagc gaatcagtga
      121 actgtacgcc atcgaggatg aaatacgggg attaccagag tcagagtgtc ttgcagtcag
      181 gcagcagcga agcaaagcgt tactgacgtc gctgcatgaa tggatggtgg agaagaatgg
      241 cacgctgtcg aaaaaatcca gactgggcga agcattcagc tatgtactga atcagtggga
      301 cgccctctgt tattacagtg atgacggtct ggcggaggcg gacaataaca cagcggaaag
      361 agcgcttcgt gcagtctgtc tcggaaagaa aaattacgtg ttcttcggta gcgatcacgg
      421 cggcgagcgt ggtgcactgc tgtacgggct gatcggcacc tgccgtctga acggtatcga
      481 tccggaagcg tatctgcgcc atattctgag cgtactgccg gaatggcctt ccaaccgagt
      541 tgacgacctc ctgccatgga aggtggtgct accatcagga taatcgtcaa tacggtgttc
      601 ggtgaacgtt tacttg
//
LOCUS       DAJTRN010000329          609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRN010000329 DAJTRN010000000
VERSION     DAJTRN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            177..>609
                     /locus_tag="OO328_004260"
     CDS             177..>609
                     /locus_tag="OO328_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR5932563.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKEL"
BASE COUNT          152 a          140 c          159 g          158 t
ORIGIN      
        1 ttcgcaccat tgcggggcgc tgtttttatt atttcctgtc tacactcagc ttattgcgac
       61 gcgaaatcgt gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg
      121 taaaagacgt ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga
      181 aaaagatgat gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg
      241 cgcatgctgg cgcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca
      301 gtgatggatt acctaggttc tcttatgccg atgcagacgg taagttttca ggtattgatg
      361 ttgatgtttg tcgtggtgtt gccgctgctg tatttggtga cgacacgaaa gtgaaatata
      421 ccccactcac agcaaaagaa cgcttcaccg ctttacagtc aggggaagtg gatttgctct
      481 cccgtaatac gacctggact tcatctcgcg atgccgggat gggaatggca tttaccggcg
      541 tcacttatta cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga
      601 aagaactgg
//
LOCUS       DAJTRN010000330          603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTRN010000330 DAJTRN010000000
VERSION     DAJTRN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>603
                     /locus_tag="OO328_004261"
     CDS             <1..>603
                     /locus_tag="OO328_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5932564.1"
                     /translation="ITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSK
                     INQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNR
                     IEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIR
                     GDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHK"
BASE COUNT          193 a          105 c          120 g          185 t
ORIGIN      
        1 attaccgcga acgaaaataa ggttggaacg acatttaaca ccactgtaac taatggatcc
       61 tcatatcttg gacgctgtca ttgtgatact ggtaacgtag gagaattccc ctatatctat
      121 tatacgtcaa aaataaacca ggcattaact tatgctgggg ttcactctaa tattaattat
      181 tatgatctta atccgaacct tgatgttggg attgctatag atattcttgg ggttgggtat
      241 gtgaatgcac cttttgaata tcatgctaac aacccgtctg gtaatacaaa gtacaattgc
      301 aaccgcattg aacctttaag tatatccagt ggggctaaag cgatagtata tttttatatc
      361 aagaagacat ttgcaggaaa attgattatt cctgaaacaa agatagtgac attgtatgga
      421 acaattagcc gtgacacccc ggtggattac tcacagccga tggctgatgt ttatattcgg
      481 ggcgatatta cagctccgca aagttgcgaa ataaataatt taaagccagt ttgttttgat
      541 tttaaagaaa taccagctgc agatttttca tctgttgttg gaagtgcggt aacaacacat
      601 aaa
//
LOCUS       DAJTRN010000331          599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRN010000331 DAJTRN010000000
VERSION     DAJTRN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..599)
                     /locus_tag="OO328_004262"
                     /pseudo
     CDS             complement(1..599)
                     /locus_tag="OO328_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT          149 a          168 c          147 g          135 t
ORIGIN      
        1 taacccgcgt tactggcgct ggtgaacata ttcaatgctg ttgaggccaa tggcagcggg
       61 aagtgcaggg ctttggctgt atcggcaacc agaccaagat ccttaacaaa aatatcgacg
      121 gctgaatgcg gggtgtaatc gccatccacc acatgacgca tccggttttc gaacattcag
      181 gaatttccgg cggcattggt cacgacgtca tacatcacat ccagcgggat ccccgcacgg
      241 gctgcaagtg ccatcgcttc ggctccggca gcaatatgta cgcccgctaa caactggtga
      301 ataattttta cggtcgaacc tagtcccggt tctgcaccta tgcgataaac ttttccggca
      361 acggcttcca gcacgggtac cagtcgttca aaggcaatat cgctaccgga ggccatgaca
      421 gtcatttcac cgttagcggc ttttactgca ccaccagaaa ctggcgcatc cagcatttcc
      481 agatcgaatc cagccagagc ggtagcaatt tcttgcgcat cagcactagc gatagtggaa
      541 gaaaccatta ctgccgtacc gggtttcaga tgttgtgcaa cgcctgtttc accaaacag
//
LOCUS       DAJTRN010000332          598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTRN010000332 DAJTRN010000000
VERSION     DAJTRN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>598
                     /gene="wcaD"
                     /locus_tag="OO328_004263"
     CDS             <1..>598
                     /gene="wcaD"
                     /locus_tag="OO328_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5932565.1"
                     /translation="QSLDAGKYVTSTMLFVYIVIIIGMVWSIRFKTISPHNHRKILRF
                     FYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVLNFIKFGGKRTTALYF
                     EPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGVMTFILFYLLEWAFQY
                     LNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
BASE COUNT          144 a          117 c          141 g          196 t
ORIGIN      
        1 cagtcgttgg atgccggtaa atacgtcact tcaactatgc tgtttgtcta tattgtgatc
       61 attattggca tggtgtggag tattcgtttt aaaacaattt cgccacacaa ccatcgtaag
      121 atattacgtt tcttttatct ggtggtcgga ctggtggtgg cgctggcggc ggtggagatg
      181 gcgcaaatta tcctcaccgg tggcagcagt attatggagt cgatttcgaa atatctgatt
      241 tacagcaaca gctatgtgct gaatttcatt aaattcggcg gcaagcgcac gacagcactt
      301 tatttcgaac cggcattttt cgctctggca ttaatctcaa tttggctcag catcaaacag
      361 tttggtatca aaacgcctaa aacagatgcc ctgattctcg cagggataat attatccgga
      421 tcgttttcag gggttatgac ctttatcctg ttttatttgc tggagtgggc atttcaatat
      481 ctgaataaag aggcgattaa gaaaaaatta ccgttagcat tgatttctct ggctgtattc
      541 cgggttggtg tggtaatcgc gtttccttat atttccaccc gtctgggcga tttaggta
//
LOCUS       DAJTRN010000333          595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRN010000333 DAJTRN010000000
VERSION     DAJTRN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..595
                     /locus_tag="OO328_004264"
                     /pseudo
     CDS             1..595
                     /locus_tag="OO328_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          227 a          108 c          107 g          153 t
ORIGIN      
        1 agaattgccg ccactgacga tggaacaaca ataactaact cagatacaaa gaaagaagcc
       61 gttctggcaa tcaataacga agactcctac atatatcatg ggaacataaa tggcaatata
      121 aaactaacgc acaatattaa ttctcaggat aagaaaacta atgcaaaatt aattctggat
      181 ggtagtgtca acacaaaaaa tgatgttgaa gtcagtaatg ccagtcttac catgtaaggc
      241 catgcaacag agcatgcaat attcagaagc acagcgagtc attgctccct ggtatttctt
      301 tgtggaacgg actgggtcac cgttttgaaa gaaacagaga gttcatataa taaaaagttc
      361 aattctgatt acaaaagtaa taatcagcag acctcatttg atcagcctga ctggaaaacc
      421 ggggtgttta aatttgatac attacacctg aacaatgctg acttttcaat atcacgcaat
      481 gccaatgttg aaggaaatat atcagcaaat aaatcagcta tcacaatcgg cgataaaaat
      541 gcttacattg ataatcttgc agggaaaaat attactaata atggttttga cttca
//
LOCUS       DAJTRN010000334          574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRN010000334 DAJTRN010000000
VERSION     DAJTRN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            154..574
                     /locus_tag="OO328_004265"
                     /pseudo
     CDS             154..574
                     /locus_tag="OO328_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          135 a          121 c          195 g          123 t
ORIGIN      
        1 ggagcgggaa tttgcgacaa ttcctgccgg tatcaacgcc gacggcatgt attgccctga
       61 atgcggtacg gttcactggc cggatggcgt tattccgcct ttctgatgct tccccgtttt
      121 gccgatattt ttcagcaggg aaaccgatgg cttaactggc tggagaagca gccggtgcag
      181 atgagccgcc tggaacatta tgccggtcag gatgaaattg gcctgcgtta caacagccac
      241 cggacaaaac gagaagaaaa ccttgtgatg agtggcgacg agtttatgga aaggttctcc
      301 tggcatgtgg cggataaggg gttccggatg gtgatacggg gtcctgagtc cggtgaagcg
      361 gcgattactg gaagatgtgg tgtacgccat aacggagaca gtgagaaaaa cggcgaggca
      421 aatcacaagg agagggatgt atcagcagtg tctgaaggtt gacccgctga agtgcgagct
      481 gcgcggaggt cagatgcggt ttacggggct gaagaggggt taccgtctgg cagagcaggt
      541 tatgatgcat gagccgctgg ctcgaatacg gtgg
//
LOCUS       DAJTRN010000335          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRN010000335 DAJTRN010000000
VERSION     DAJTRN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..539
                     /gene="bamD"
                     /locus_tag="OO328_004266"
     CDS             <1..539
                     /gene="bamD"
                     /locus_tag="OO328_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5932566.1"
                     /translation="SQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTHPNIDYVMYM
                     RGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYTTDATKRLVF
                     LKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLMENAYRQMQM
                     NAQAEKVAKIIAANSSNT"
BASE COUNT          152 a          152 c          142 g          125 t
ORIGIN      
        1 attcgcagca ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt
       61 tagcgcaggc tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt
      121 atgtcatgta catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt
      181 tctttggcgt tgaccgtagc gatcgcgatc ctcaacatgc acgagctgcg tttagtgact
      241 tttccaaact ggtgcgcggc tatccgaaca gtcagtacac taccgatgcc accaaacgtc
      301 tggtattcct gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tactatacag
      361 aacgtggcgc atgggttgcc gtcgttaacc gcatagaagg catgttgcgc gactacccgg
      421 atacccaggc tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga
      481 tgaatgcgca agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac
      541 agaaacctga aacacaaaac ggcagccctt g
//
LOCUS       DAJTRN010000336          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRN010000336 DAJTRN010000000
VERSION     DAJTRN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..89)
                     /locus_tag="OO328_004267"
     CDS             complement(<1..89)
                     /locus_tag="OO328_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5932567.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSN"
     gene            complement(80..382)
                     /locus_tag="OO328_004268"
     CDS             complement(80..382)
                     /locus_tag="OO328_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5932568.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          160 a          132 c          116 g          163 t
ORIGIN      
        1 cagttactta gcacctcatt tccacagtca aatggctgaa gtatatgctc atccgtcaac
       61 aatgctggcg cggtgacatt tatttccatt caggctttac cgccatcaga cgttcacgcc
      121 cttctgcatt ctgcacagga gcttccagtt gcgtaataaa cgaatcataa cgttcgttat
      181 ccagcacaaa taaacgttga tccaggataa tgtttttagc ttcctggtac gccagttcca
      241 tgatgaaatc ggtacggttc ttattgacca agctcgcagc ataatcaata acggctcgtt
      301 cctcatcagt tgcccggatg ttaatctgat gttctcgcgg tgttttacgg acgcttgcag
      361 ctgtagacat aaaaacctcc attggtaaca atttgctatg caattaatgt tatcgcaaat
      421 ggtaacagaa cgctatacag aatcacttac gggaaaagcg atcccctgat aaaaacaatg
      481 gcagagacct gaacattgtg acgatgatct gtgtcggcgc acaaatcatg tctgattacg
      541 gccgtttaat gtgattttga taatgaaatg c
//
LOCUS       DAJTRN010000337          570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRN010000337 DAJTRN010000000
VERSION     DAJTRN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            53..>570
                     /locus_tag="OO328_004269"
     CDS             53..>570
                     /locus_tag="OO328_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5932569.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKT
                     GIKMKAETETGY"
BASE COUNT          167 a          120 c          152 g          131 t
ORIGIN      
        1 ggcgcttttt caacgagcaa taaacacaca ggcctatcta aaagcaggaa ttatggggtt
       61 tgctggcgac ggcaaaacct acacagcaag cgaactggct attggtctgg taatgcttat
      121 gcggcaacgg ggaattgaag ctggtaaccg cccggtaatg tttctggaca cagaaacagg
      181 ctctgactgg gttaaacccc gctttgatgc ggaaaatatc gaattattta cggcaaaaac
      241 ccgctcgttt gttgatctgc ttgaagccat taatgaagcg gaaagcagcg gttccgtaat
      301 gatcatcgac tccatcagtc acttctggac cggattatgc gacgagtatg ccagacgccg
      361 aaaccgtaaa cgaggactgg aattttcaga ctgggcgtgg ctcaaacagg agtggcgacg
      421 ctttaccgat cgatttgtta acagtcaggc gcacattatt atgtgcgggc gtgcaggata
      481 tgagtatgac tttttcgaag gtgatgatgg caaacgccag ttggcaaaaa ccggtataaa
      541 aatgaaagca gaaacagaaa ccggttatga
//
LOCUS       DAJTRN010000338          546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRN010000338 DAJTRN010000000
VERSION     DAJTRN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..537)
                     /gene="arsB"
                     /locus_tag="OO328_004270"
     CDS             complement(<1..537)
                     /gene="arsB"
                     /locus_tag="OO328_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5932570.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVM"
BASE COUNT          157 a          171 c          138 g           80 t
ORIGIN      
        1 catcaccgag gcgtattcgc gaaagccgag gccaaagaaa tcagcggaaa cgatattcac
       61 caggttggag acaataagcg gcaggctggc ggtatcggca atgaatccgg ccgccatcac
      121 gaacgccagc gtcgtgcctt tactgaatcc taaagccagc agcatggcga tgacaatcgg
      181 cgtcaaaata agcgccgcgc catcattggc aaacagggcc gcaacggcgg caccgagcag
      241 gacaatccag gtaaacagca agcgaccgcg tccattgccc cagcgtgaaa cgtgcagcgc
      301 tgcccattca aaaaagccgg actcatccag cagcaggctg atgataatga cggcaataaa
      361 cgccgccgtc gcgttccaga cgatattcca caccaccgga atatcacccg gatgaaccac
      421 gcccgtaacc aacgccagta ccgcgccgag cgttgcactc cagccgatgc ctaaaccttt
      481 cggctgccag ataaccaata cgatggtcag gacaaagata gcgcctgcca gtaacataat
      541 gcctcc
//
LOCUS       DAJTRN010000339          540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRN010000339 DAJTRN010000000
VERSION     DAJTRN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            72..233
                     /locus_tag="OO328_004271"
     CDS             72..233
                     /locus_tag="OO328_004271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932571.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            383..>540
                     /locus_tag="OO328_004272"
     CDS             383..>540
                     /locus_tag="OO328_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5932572.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
BASE COUNT          135 a          113 c          163 g          129 t
ORIGIN      
        1 accccttata ccctgatgca ggcgattgca gcagaaggtg atgtggtggt cagtggtgca
       61 actgagccgg agtgatacta cgtcatcgta ctggcccggc attccaggcc ataaaagaca
      121 gtctggcagt gggactaaat gcactgacgc tgacggatat taccaaaaat gcaacgtatg
      181 gcgttgagat agaaagtctg gtgctggaga taaatgcacc ggcatcatca taaaaagtga
      241 gccagtcaaa tggaaggtat cgttaaactc accggtagtg tcagtgggtc gtctgagacg
      301 cttgcatgag ttatcagagc catcagtagt taactggtgg cttttttatt gttgtcagct
      361 tccggataac gggagacggg gtatggacca gatggaaaaa atcacaacag gtgtgtcata
      421 caccacgtca gcggtgggaa cgggctactg gttcctgcag ttgctggaca gggtttcccc
      481 gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg tttgggctgc tgacatatct
//
LOCUS       DAJTRN010000340          535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRN010000340 DAJTRN010000000
VERSION     DAJTRN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..206
                     /locus_tag="OO328_004273"
     CDS             <1..206
                     /locus_tag="OO328_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR5932573.1"
                     /translation="FWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRYALLD
                     YIHETTIRSLDAYPVHSEHQNLN"
     gene            271..>535
                     /locus_tag="OO328_004274"
     CDS             271..>535
                     /locus_tag="OO328_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5932574.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMF"
BASE COUNT          167 a           97 c          114 g          157 t
ORIGIN      
        1 atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta ccaacccagg
       61 tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgggtac cgaaaacgat
      121 acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca tatcctgtac
      181 actcagaaca tcagaattta aattaaggtt tgatgtattc tctgaataaa acagtaatga
      241 taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga gtttattcct
      301 gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct tttgggaatg
      361 attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc aaaaataggg
      421 cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa tttatcgctt
      481 tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat gtttg
//
LOCUS       DAJTRN010000341          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRN010000341 DAJTRN010000000
VERSION     DAJTRN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>534
                     /gene="metG"
                     /locus_tag="OO328_004275"
     CDS             <1..>534
                     /gene="metG"
                     /locus_tag="OO328_004275"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HCR5932575.1"
                     /translation="KPVLPKLTERAEAFLNTELTWDGIQQPLLGHKVNPFKALYNRID
                     MKQVEALVEASKEEVKATTAPVTGPLADDPIQETITFDDFAKVDLRVALIENAEFVEG
                     SDKLLRLTLDLGGEKRNVFSGIRSAYPDPQALIGRHTIMVANLAPRKMRFGISEGMVM
                     AAGPGGKDIFLLSPDAG"
BASE COUNT          128 a          141 c          146 g          119 t
ORIGIN      
        1 tgaagcctgt actgccgaaa ctgaccgagc gtgcagaagc cttcctcaat acggaactga
       61 cctgggatgg tatccagcaa ccgctgctgg gccacaaagt gaatccgttc aaggcgctgt
      121 ataaccgcat cgatatgaaa caggttgaag ctctggtgga agcctctaaa gaagaagtga
      181 aagccactac cgcgccagtt actggcccac tggcagatga tccgattcag gaaaccatca
      241 cctttgacga cttcgccaaa gttgacctgc gcgtggcgct gatcgaaaac gcagagtttg
      301 ttgaaggttc tgacaaactg ctgcgcctga cgctggatct cggcggtgaa aaacgcaatg
      361 tcttctccgg tattcgttct gcttacccgg atccgcaggc actgattggt cgtcacacca
      421 ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg tatctctgaa ggcatggtga
      481 tggctgccgg tcctggcggg aaagatattt tcctgctaag cccggatgcc ggtg
//
LOCUS       DAJTRN010000342          531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRN010000342 DAJTRN010000000
VERSION     DAJTRN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..531
                     /locus_tag="OO328_004276"
                     /pseudo
     CDS             1..531
                     /locus_tag="OO328_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          136 a          130 c          142 g          123 t
ORIGIN      
        1 ggtggtcaac aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
       61 cgtgctctgg cacgtcagaa tgagaatcat aaggccggta ataaatacat cccttacggg
      121 aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat
      181 tctccctcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
      241 ttccggaagc gggctgcttt tccccgattc aaaaagcggt gacagaatga tgcattccgc
      301 tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc
      361 tggatgcgct accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc
      421 cagtcctgcg gtacgtggta catcagtatt cagacagaaa gtgaagtatc cactccggct
      481 cacccttcag catcaatggt cgggctggat gctggcgtgg ctaaactcgc c
//
LOCUS       DAJTRN010000343          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRN010000343 DAJTRN010000000
VERSION     DAJTRN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(108..>528)
                     /locus_tag="OO328_004277"
     CDS             complement(108..>528)
                     /locus_tag="OO328_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5932576.1"
                     /translation="LPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDA
                     MVFDGLRLMKNGYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAY
                     AQAIMGHSSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          150 a          152 c          101 g          125 t
ORIGIN      
        1 cctatagtga gtcgtattaa ttatcccttg acaagtagag aaaactatga gtcaaaaaca
       61 tgaccacaca ggtctgtagt aagtgtcatc ctagactatg gtctcctcta ggtctcctta
      121 taaacatcag ccatcacatt gaccagccta tcaacctcga cctctttagc ataacggtcg
      181 tacgtaatag accctgaact atgccccatg atcgcctgtg catacgccaa ctcgacccca
      241 tgttgtttta actgggccgc aactgtatgt ctaaacgagt gaaacgctaa gccgctacgg
      301 tctacgagga ctttaggcaa caacgtccga ttaaaccatt tactcacctg ctcaccgtaa
      361 ccgttcttca tgagacgcag accatcgaac accatagcgt catcaccgtc agcgcctcta
      421 cgggtctcta caagcgccat aaagtcagcc aatataaaac cataggcccc atcgaccaga
      481 ggcacacagc ggtcactatg agcgttcttg atgctcttac cgggtaga
//
LOCUS       DAJTRN010000344          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRN010000344 DAJTRN010000000
VERSION     DAJTRN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..258)
                     /locus_tag="OO328_004278"
     CDS             complement(<1..258)
                     /locus_tag="OO328_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCR5932577.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVN"
     gene            complement(255..338)
                     /gene="idlP"
                     /locus_tag="OO328_004279"
     CDS             complement(255..338)
                     /gene="idlP"
                     /locus_tag="OO328_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5932578.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT          160 a          118 c          108 g          139 t
ORIGIN      
        1 attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg
       61 gcaataccag ctctggagat gtttgtcgct cgggttatgc acttgccact cgggcatgcc
      121 tgaaacaagt aatgacggta atgccgaatg aggagaatta aaaagagtga ggagatcgct
      181 gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta aaacagcctg
      241 aagtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct
      301 gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta
      361 catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa ccttacttgc
      421 ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact
      481 caaaaaaaaa acgctagtat attctgaata ttccgaatag ttacg
//
LOCUS       DAJTRN010000345          522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRN010000345 DAJTRN010000000
VERSION     DAJTRN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..522
                     /locus_tag="OO328_004280"
                     /pseudo
     CDS             1..522
                     /locus_tag="OO328_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          129 a          148 c          146 g           99 t
ORIGIN      
        1 gttactgccg cagcccgtga actcagcctg tatgaatcac agctctacaa ctggcgcagt
       61 aaacagcaaa atcagcagac gtcttctgaa cgtgaactgg agatgtctac cgagattgca
      121 cgtctcaaac gccagctggc agaacgggat gaagagctgg ctatcctcca aaaggccgcg
      181 acatacttcg cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat caggctgagt
      241 tcagcatcaa agcaatgtgc cgcgtgctcc gggtggcccg cagcggctgg tatacgtggt
      301 gtcagcggcg gacaaggata agcacgcgtc agcagttccg ccaacactgc gacagcgttg
      361 tcctcgcggc ttttacccgg tcaaaacagc gttacggtgc cccacgcctg acggatgaac
      421 tgcgtgctca gggttacccc tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg
      481 gactgagggc aaaggcctcc cggaagttca gcccggtcag ct
//
LOCUS       DAJTRN010000346          521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRN010000346 DAJTRN010000000
VERSION     DAJTRN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            92..>521
                     /gene="mcbR"
                     /locus_tag="OO328_004281"
     CDS             92..>521
                     /gene="mcbR"
                     /locus_tag="OO328_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5932579.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIY"
BASE COUNT          158 a          106 c          129 g          128 t
ORIGIN      
        1 gggtggttta tgattattca ctttaataca ctaggtgaac tccttctgcc atgcaggcag
       61 ggttggacag aaaacacgcg aaggaattaa aatgcctgga acgggaaaaa tgaaacatgt
      121 cagtttgact ctgcaggttg agaacgacct gaaacatcag cttagtattg gcgcattaaa
      181 acctggcgca cgcctgatta ctaaaaatct ggcggagcaa ttaggtatga gtattacacc
      241 tgtgcgtgaa gcattattac gtctggtttc ggtgaatgcg ctttctgtcg cacctgcaca
      301 agcatttaca gtaccggaag tgaggcaaag tcagttagac gaaatcaatc ggatccgcta
      361 cgaactggaa ttaatggcag ttgccctggc tgttgaaaac ctctcctcgc aagacatagc
      421 agaacttcag gaattgctcg ggaaattaca gcaggcgcaa gaaaagggcg acatggaaca
      481 aatcattaat gtgaacaggc tatttcgctt agcgatttat c
//
LOCUS       DAJTRN010000347          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTRN010000347 DAJTRN010000000
VERSION     DAJTRN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>518)
                     /locus_tag="OO328_004282"
     CDS             complement(<1..>518)
                     /locus_tag="OO328_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5932580.1"
                     /translation="GSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDNLQVYSKE
                     QSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQGENGD
                     IGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDNDTGFKQ
                     NGDGILDVYANS"
BASE COUNT          133 a          150 c          102 g          133 t
ORIGIN      
        1 gactattagc ataaacatcc agaataccgt ctccattctg tttaaatccg gtgtcattat
       61 caccaagaac aattgaattc ccccccaggg cgttctgaac gccgataccc agcgcaccat
      121 tgacctgcga accaccgcca acagacactt tatgcgacat ggatatttca cccgtccgca
      181 aattaatggt gaacgggcgc aggggaccaa tatcgccatt ttccccctga ccttcactgg
      241 tgggaataag gtgcaggcac tcttccgaac gacgaaaaat caggccaaaa gcttcgttga
      301 aaatcctcag cgcattaaca ccacggattt tcagctctcc ggttatttta tcgccttccc
      361 gatgaacata gcgttgatct gactgctctt tactgtaaac ctgaagatta tcccgtgccg
      421 tccctttatt ctgaaggtct gacagattgt tcttctgccg caggaatatc cttttcggat
      481 cctcaagaag atttacccag cccgcactgt cggagcct
//
LOCUS       DAJTRN010000348          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRN010000348 DAJTRN010000000
VERSION     DAJTRN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            35..>518
                     /locus_tag="OO328_004283"
     CDS             35..>518
                     /locus_tag="OO328_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5932581.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     L"
BASE COUNT          132 a          130 c          154 g          102 t
ORIGIN      
        1 tgcgatcccg ccaggcttca gggggtaaca gaccatgaaa aatattgcga caggaggcgt
       61 tctggagcgt atccgcagac tgaccccacc acatgtaacc gccccattca gaacggttgc
      121 ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt tgcgaggaga tcaaccgcct
      181 gaatcgtcag ttgcgggtgg aaaaaatcct gaaccgctcc ggcatccagc cgttgcaccg
      241 gaagtgttcg tttgcgaatt accgggcgca gaacgacggt cagcgacatg cactgagtca
      301 ggcgaaatcc atagctgacg aactgatgac aggctgtacg aattttgtgt tcagcggtaa
      361 gcccggcacc ggaaaaaatc atcttgcagc cgcgattggc aaccggctga tggcgaaggg
      421 gcgtagcgtg attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg agagctacga
      481 caacggcaaa tccggtgaaa aatttttaca ggagcttt
//
LOCUS       DAJTRN010000349          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTRN010000349 DAJTRN010000000
VERSION     DAJTRN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..248)
                     /locus_tag="OO328_004284"
     CDS             complement(<1..248)
                     /locus_tag="OO328_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932582.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFC"
     gene            273..>511
                     /locus_tag="OO328_004285"
     CDS             273..>511
                     /locus_tag="OO328_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5932583.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
BASE COUNT          146 a          105 c          124 g          136 t
ORIGIN      
        1 aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt cacaaagtat
       61 atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg acctgcacaa
      121 tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat caccgttctt
      181 acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg cgatacttgt
      241 aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg cgtattgacg
      301 atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact ccttctgaag
      361 cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc aaacagacac
      421 tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt cgtaatccta
      481 agccagtacg tgtgacgctg gatgaactct g
//
LOCUS       DAJTRN010000350          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRN010000350 DAJTRN010000000
VERSION     DAJTRN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..511
                     /locus_tag="OO328_004286"
                     /pseudo
     CDS             1..511
                     /locus_tag="OO328_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAJTRN010000351          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRN010000351 DAJTRN010000000
VERSION     DAJTRN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            6..>508
                     /locus_tag="OO328_004287"
     CDS             6..>508
                     /locus_tag="OO328_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5932584.1"
                     /translation="MFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILGVKEYICD
                     KPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVF
                     ESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLN
                     KHGQWKKL"
BASE COUNT          109 a          142 c          152 g          105 t
ORIGIN      
        1 aacgtctgtt cacggccaac cagtgctgga catccttcct ggatgcgggc ggtctgcgcg
       61 atatcgaggt tgaggctgtc acaaaaatgc tggcctgcgg cacgcggata ctgggcgtga
      121 aggaatacat ctgcgataaa cctgagtgcc cccacgtcag atacgtcact aactcatgcg
      181 gcagccgtgc ctgcccgtcc tgcggaaaaa aggccacaga cctgtggata gcgacacagc
      241 tgaatcgtct tcctgactgc gactgggtac acctggtctt caccctgccg gacacgcagt
      301 ggccggtgtt cgaaagcaac cggtggctgc tgaatgacgt gtgccgtctg gcggtggaga
      361 atctgctgta tgccgcccgg aaacgggggc aggaacccgg tatcttctgc gccatccaca
      421 cgtatggccg tcgtctcaac tggcatccgc atgtacatgt gtctgtaacc tgtggaggtc
      481 tgaataagca tggtcagtgg aaaaagct
//
LOCUS       DAJTRN010000352          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRN010000352 DAJTRN010000000
VERSION     DAJTRN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..143)
                     /locus_tag="OO328_004288"
     CDS             complement(<1..143)
                     /locus_tag="OO328_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA-like transporter family protein"
                     /protein_id="HCR5932585.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVL"
     gene            complement(140..>508)
                     /gene="mddA"
                     /locus_tag="OO328_004289"
     CDS             complement(140..>508)
                     /gene="mddA"
                     /locus_tag="OO328_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5932586.1"
                     /translation="YPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGK
                     GLGRKLLSRLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHW
                     LDLTFMQLQLDERTEPDAIG"
BASE COUNT          135 a          145 c          121 g          107 t
ORIGIN      
        1 agcacaatcc ccaccagtga gttcagcaac atggcgatga gaatggtaga ggaggtctgg
       61 gtgatgcgcg ccatcagcgt gttctggact accagaccaa ttccggcggc aatcaaaaaa
      121 gcaagggtga gcgactgatt catccaatcg cgtccggttc agtgcgctcg tcgagttgca
      181 actgcataaa tgtcagatcc agccaatgac caaatttagt gcctacctgc ggcatttgtg
      241 cggtgacgac aaatcccagc gactggtgca gatgcagcga agcctgattt tgcgattcga
      301 tcccggcgac catgacatgc ttcccgcaat cccgcgcttc atcaatcaat cgacttaaca
      361 atttacgccc cagacctttg ccctgatgat cgggatggac ataaaccgaa tgttccacgg
      421 tatggcgaaa accatcgaaa ctacgccagt cgccaaacga ggcatatccc gtaactacgc
      481 catcttcttc acttaccagc accggata
//
LOCUS       DAJTRN010000353          500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRN010000353 DAJTRN010000000
VERSION     DAJTRN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            1..500
                     /locus_tag="OO328_004290"
                     /pseudo
     CDS             1..500
                     /locus_tag="OO328_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          131 a          127 c          130 g          112 t
ORIGIN      
        1 cccgtaatgc ccggaatatt ctggaagcca tccggggtga aatactctcc acggtagaaa
       61 acgtggtgga tgtttacgtc acccataatc cgaaaaaaac agaacaaaaa gccggagtca
      121 gtatccatta acacccggtt cgctttatgt tggcgtgtac ggcggcagcc cggcagatat
      181 cgcggcggcc atctggcgta aggctccgcc gggtattgat atgaatggca acacaacgtt
      241 cactgttgca gataaggagt acgatccgcc gtatcctgaa tacgtgatca gctggcagac
      301 actcaaaccc gtcagtctgc atgtcagtgt gacgctgaaa aaaagtgact acctgccttc
      361 agatattacc cgacaggtac agcaatccgt gctggacgca tttaatggta cagatggtgg
      421 tctgcgggca agagttgcct ctgttgtctc tgccgggcgc tactatgccg gcatttacaa
      481 aaccgatccg gaacatattg
//
LOCUS       DAJTRN010000354          500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTRN010000354 DAJTRN010000000
VERSION     DAJTRN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..181)
                     /locus_tag="OO328_004291"
     CDS             complement(<1..181)
                     /locus_tag="OO328_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5932587.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDA"
BASE COUNT          191 a           91 c           78 g          140 t
ORIGIN      
        1 tggcatcgcg gccaattagt tgtggacaaa gatgatcttt cagataagaa gcgaccgaca
       61 attcgcgacc atttaacgtg gcatctccta atccacaaag tccgctttca gtggttattt
      121 taagcgtaac gaaattacgc cctgggcaag taacaaaaac atctgcagca ataattttca
      181 ttattgcaat ctcctgtgtt gtaaatagac gtttcaaacc taattttacg cctactacca
      241 tacaagagag aaaaagcgat tattcaaccg tgatcacatt aaaaacaaaa atttctaccg
      301 aggagaaaaa ataccctcaa aaaccacctg aataattaac cattattgtt aaaacaacac
      361 gttgaataat cacataacaa actccattaa tcattgaaaa tgagcacaga aatagaaatt
      421 aattatattc aattaattaa aatgtgtatt gcttgatgta tttaaaaata ttattttcat
      481 ggtgagagtg gaggtaaaga
//
LOCUS       DAJTRN010000355          499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTRN010000355 DAJTRN010000000
VERSION     DAJTRN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>499)
                     /gene="yidZ"
                     /locus_tag="OO328_004292"
     CDS             complement(<1..>499)
                     /gene="yidZ"
                     /locus_tag="OO328_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5932588.1"
                     /translation="FDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQT
                     PRGLKFELAAESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFS
                     GRESHPRSRELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISIC
                     WEQSDT"
BASE COUNT          123 a          130 c          128 g          118 t
ORIGIN      
        1 aggtgtcgct ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat
       61 tccacgtttc atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa
      121 acagcacttc ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat
      181 ggctttcgcg accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat
      241 cgtaatccca gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg
      301 acagcgcatt aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc
      361 cgcgcggtgt ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg
      421 ccagattttg ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca
      481 caaagagcgg gtcgtcaaa
//
LOCUS       DAJTRN010000356          498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTRN010000356 DAJTRN010000000
VERSION     DAJTRN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..438
                     /gene="curA"
                     /locus_tag="OO328_004293"
     CDS             <1..438
                     /gene="curA"
                     /locus_tag="OO328_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5932589.1"
                     /translation="DHHADDFAEQLAKACPKGIDIYYENVGGKVFDAVLPLLNTSARI
                     PVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYGHRIHEFQKEMGQW
                     VKEDKIHYREDITDGLENAPQTFIGLLKGKNFGKVVIRVAGDD"
BASE COUNT          138 a          106 c          130 g          124 t
ORIGIN      
        1 gatcaccacg cggacgattt tgccgaacaa ctggcgaaag cgtgcccaaa aggtattgat
       61 atctattatg aaaacgtggg cggtaaggta tttgatgcag tgctaccatt acttaataca
      121 tctgcgcgca ttcccgtttg tggcttagtg agcagctata acgctacaga gctaccaccc
      181 ggtccggatc gtttacccct gttgatggca acggtgctga aaaaacgtat tcgcttgcaa
      241 gggtttatta tcgctcagga ttatggtcac cgcatccatg agtttcagaa ggagatgggg
      301 caatgggtga aagaggataa aatccactac cgcgaagata ttactgatgg tttagagaat
      361 gcgccacaga cgtttatcgg cctgctgaag ggtaaaaact tcggcaaagt ggtgatccgc
      421 gtcgcgggtg atgattaaag tttcaacggc ggcgtaagcc gccataaaaa tccaggcaaa
      481 aagcttattt tcagcttt
//
LOCUS       DAJTRN010000357          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTRN010000357 DAJTRN010000000
VERSION     DAJTRN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          176 a           98 c           70 g          153 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgc
//
LOCUS       DAJTRN010000358          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTRN010000358 DAJTRN010000000
VERSION     DAJTRN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..473
                     /locus_tag="OO328_004294"
     CDS             <1..473
                     /locus_tag="OO328_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5932590.1"
                     /translation="QALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIEL
                     SPYLQNRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMG
                     EGYSVIPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          138 a          115 c          119 g          121 t
ORIGIN      
        1 tgcaggcgct gctggaagcc aaaaaacaag ggctgacgcg tgagatcggt atttccaact
       61 tcacgatccc attgatggaa aaagtgattg ctgctgttgg cgctgaaaac atcgctacca
      121 accagattga actctctcct tatctgcaaa accgtaaagt ggttgcctgg gctaaacagc
      181 acggtattca tatcacttcc tatatgacgc tggcttatgg taaggcactg aaagatgagg
      241 ttattgctcg tattgcagct aaacacaatg cgattccggc acaagtgatt ctggcgtggg
      301 ctatggggga aggttactca gtaattcctt cttctactaa acgtaaaaac ctggaaagta
      361 atcttaaggc acaaaattta cagcttgatg ccgaagataa aaaagcgatc gccgcactgg
      421 attgcaacga ccgcctggtt agcccggaag gtctggctcc tgaatgggat taagcctctc
      481 tgacagctcc tcc
//
LOCUS       DAJTRN010000359          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTRN010000359 DAJTRN010000000
VERSION     DAJTRN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>490
                     /locus_tag="OO328_004295"
     CDS             <1..>490
                     /locus_tag="OO328_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5932591.1"
                     /translation="NERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYT
                     SYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAK
                     WRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDI
                     DS"
BASE COUNT          137 a          114 c          122 g          117 t
ORIGIN      
        1 acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc acaaaaacat
       61 ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt ggtcgcgatg
      121 ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg actacgcaga
      181 aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc gcaacctttg
      241 aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt gcccatcagg
      301 atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat acccgtaatt
      361 tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg gcacaattgc
      421 gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg gtcggcgata
      481 tcgacagtaa
//
LOCUS       DAJTRN010000360          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTRN010000360 DAJTRN010000000
VERSION     DAJTRN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>490
                     /locus_tag="OO328_004296"
     CDS             <1..>490
                     /locus_tag="OO328_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5932592.1"
                     /translation="MLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESAEIAEAL
                     KVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQMAESGVTLHADAAALTQLQAG
                     PAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRF
                     VNE"
BASE COUNT          134 a          118 c          131 g          107 t
ORIGIN      
        1 catgctgatc ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat
       61 cccggtgatc acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgcagagat
      121 cgctgaagca ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac
      181 ggttgaaacg ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa
      241 acaaatggcg gaaagcggcg tgacattaca cgcagatgca gctgcgctga cacagttgca
      301 ggcaggccct gcgaaggtgg tggcggttaa agccgaagag tatgacgatg agtttctgtc
      361 attagatttg aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga
      421 acacggcaca caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt
      481 tgttaacgaa
//
LOCUS       DAJTRN010000361          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTRN010000361 DAJTRN010000000
VERSION     DAJTRN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..334
                     /locus_tag="OO328_004297"
     CDS             <1..334
                     /locus_tag="OO328_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HCR5932593.1"
                     /translation="YPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQLPAQCRSCNVL
                     KACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGRPASDIMQ
                     AHLMVVNK"
BASE COUNT          119 a          111 c          136 g          123 t
ORIGIN      
        1 ttatccgcaa tatcggctgg ggaatatgca tcagcaaaca attgcagaaa tgatcgattc
       61 cccgcaacag caggtgtttg gtgaagataa atttaagcaa ttaccggcgc agtgtcgcag
      121 ttgtaacgtg ttaaaagcat gttggggagg ctgcccgaaa caccgcttca tgctcgatgc
      181 cagcggcaaa ccggggctga attatttgtg tgccgggtat cagcgttatt tccgccatct
      241 accgccatat cttaaagcaa tggctgattt gctggcgcac ggtcgcccgg ccagtgacat
      301 tatgcaggca catttgatgg tggtgaataa gtaaaaaagc ggccatttgg aagccgcctg
      361 cggttgattg ccggatgcgg cgtaaacgcc ttatccggcc tacatgatcg tgcaaattca
      421 ataaattgca gcgttctgtg gatactggtt tgggtgatcg tatccggcaa agcctttacg
      481 tgcggtggc
//
LOCUS       DAJTRN010000362          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTRN010000362 DAJTRN010000000
VERSION     DAJTRN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            206..>488
                     /locus_tag="OO328_004298"
     CDS             206..>488
                     /locus_tag="OO328_004298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5932594.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          142 a           91 c          118 g          137 t
ORIGIN      
        1 ccagtgcgta agtgttagtg tcagctcctg ccatttcagc agaggaaatt attctgctga
       61 tgaaagcttt ggcatcagga ttcagcctca aacgtatgcg tggtcgtcag aggcagttag
      121 gtcagaagca ataattttag acagtgagac catgtgcatt ggttttaaaa atgccgtcta
      181 tgttcatgat tgccttgatt tgcatatgga acaactggat ttagattatt gcggctcaac
      241 aggcgtggtt atagagaatg taaacggagg attttctttc tcaaactcat ggatagcagc
      301 agatgccgat ggcactgaac aatttacggg gatatatttt agaacgccgg cctcaacgca
      361 gtcacataaa attgtcagtg gtgttcatat caacactgca aacaaaaaca cggctgcaaa
      421 caatcaaagt atagcgatag aacagtcggc gatcttcgtc tttgtaagtg gttgtacgtt
      481 aactggtg
//
LOCUS       DAJTRN010000363          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTRN010000363 DAJTRN010000000
VERSION     DAJTRN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..90
                     /locus_tag="OO328_004299"
     CDS             <1..90
                     /locus_tag="OO328_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932595.1"
                     /translation="DEKPETIQPQSRPSSAITVSKTGYVFFDR"
     gene            198..>486
                     /locus_tag="OO328_004300"
     CDS             198..>486
                     /locus_tag="OO328_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932596.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDH"
BASE COUNT          138 a          104 c          131 g          113 t
ORIGIN      
        1 gacgagaagc cagaaaccat tcaaccacaa tccagaccat catccgcaat caccgtatcg
       61 aaaactggct atgtgttttt cgacaggtga accatgaaat caaaaatcaa atcgctactg
      121 gtcgctggtt ataaccacgg ctggttaagt atttcgtttg tcgatttctg gtttaaaaat
      181 ctcaatctga gggaatcatg aggccaagtg aacttagcga cctgctttgg gcgcaggttg
      241 acagggtggc tccgcacctg ttgccaaacg gcaagataga ggggcatgag tgggttgccg
      301 gtaacgtcaa cggtgacaag ggaaacagcc ttaaggtcaa ccttatcggc aagaaaaaat
      361 gggctgattt cgctgaggga gacggcggtg acatgcttga tttgtggatg gcatgtcgtg
      421 gaattaacct gcatcaggct atgcaggaag cgaaagcatt tctcggtatc aaggatgacg
      481 atcacc
//
LOCUS       DAJTRN010000364          482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTRN010000364 DAJTRN010000000
VERSION     DAJTRN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>482)
                     /locus_tag="OO328_004301"
     CDS             complement(<1..>482)
                     /locus_tag="OO328_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5932597.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     "
BASE COUNT          129 a          149 c          111 g           93 t
ORIGIN      
        1 aggaatcgcc gctacccaac cggttttcgc cagactgaaa ccttgttcgg cttgcaaata
       61 accgggtaac cacgcaatgt aaagccaacc ggtataattg acgccagaga aacctaaaat
      121 catcccccag gttgtccgat gcttaaacag cgccagccac tcgctaaatt gcagctgtgg
      181 acgcggttta accgacgcgg agagatacgt ccgttcttcg tcagtcagga caaactgcgc
      241 ccggttgcga taccacgcgt accaacagat cccaaccaga atccctgcca cgccgatgat
      301 aacgaacatc gtccgccagc cccatgccag ctgcatcaat acaagagcag gaggcgcgat
      361 agcctgaccg ataacggtag atgagttaaa aatccccagc gccgtgccgc gttctttttg
      421 tgcataccag tcggtgatcg actttactcc cgcaggcata aatggcgctt cgccaatacc
      481 ca
//
LOCUS       DAJTRN010000365          475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTRN010000365 DAJTRN010000000
VERSION     DAJTRN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..297
                     /gene="sitD"
                     /locus_tag="OO328_004302"
     CDS             <1..297
                     /gene="sitD"
                     /locus_tag="OO328_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5932598.1"
                     /translation="IVATLKAVGIILSISLLIAPGAIAVLLTQRFHIALLLATGISVI
                     VSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNAHTQDLLSPN"
BASE COUNT          123 a          130 c          104 g          118 t
ORIGIN      
        1 attgtggcga cgctgaaagc agtaggtatc attctctcta tttctctgct cattgcgcct
       61 ggcgccattg cagtactcct gacacaacgc tttcatattg cgctcctact ggcgactggc
      121 atatcggtaa tagtgtcaat gactggtgtc tggctgtcct tttttatcga cagcgcacca
      181 gctccgacga ttgtcgtctt attcgcggtc gtgttcatca tgacgtttac cgttaccagc
      241 atcaacgcac gcacaaaggg aaacgcccat acacaggatc tgttatcacc caattagcac
      301 aacatccgag gacagcatcg tccgccagcc tacagaagtc cggaaagcca tggaaggtcg
      361 ttgggaggag gtacgaattc tatcatgcaa aaaatacgta aaatcgataa cgcctagaaa
      421 tcattcaata ctcgcactat cgaaagttta ccagccaacc gcggcacgtt cctgc
//
LOCUS       DAJTRN010000366          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTRN010000366 DAJTRN010000000
VERSION     DAJTRN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(117..>473)
                     /locus_tag="OO328_004303"
     CDS             complement(117..>473)
                     /locus_tag="OO328_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5932599.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          117 a          119 c           84 g          153 t
ORIGIN      
        1 ttaacgtcct gtgccatttt tcaggcataa aaaaaccgct cggcggcggg ttttgcttac
       61 tttaccatcg cgtacaaaat cggcaaagta tcagatttgc atgagatata tgccttttaa
      121 tctacttttg caatactttg ctttgaaaat gccgcttttt gttctgaacg tgttctcatc
      181 acaaacaata aagcctcact atccagtcgg tgaaaaatgt gtttcattac aacccagtga
      241 ccagtaaatg ttttggacca gtttttggtt gttactcccg ccagtaatgc cagctcctga
      301 tattcataac cttccccacc aaaaagttct gcttttaccg cctgcgccgc cagccagatt
      361 aattttttca ggcgttcctg tgttttctct gcaattttcc tagtgccaga atgagcctta
      421 aactcttccc acgcccagtg ggttatggtg atctgatact cccagcaaat act
//
LOCUS       DAJTRN010000367          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTRN010000367 DAJTRN010000000
VERSION     DAJTRN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..361
                     /locus_tag="OO328_004304"
     CDS             <1..361
                     /locus_tag="OO328_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932600.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAJTRN010000368          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTRN010000368 DAJTRN010000000
VERSION     DAJTRN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>450
                     /locus_tag="OO328_004305"
     CDS             <1..>450
                     /locus_tag="OO328_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5932601.1"
                     /translation="TLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPE
                     FNRLNFKPVTYTDAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAE
                     LRQNNTPPADKMIPGDGRTLVVHFDKFGNIRFTETVPDGALVCTLDT"
BASE COUNT          119 a          126 c          100 g          105 t
ORIGIN      
        1 taccctttct caccctgacg caaccatcga aaatggtcgc gctgtcacta cgtctgttgc
       61 ggttgcggag ttcttccgga aaatgcacaa aaacgttatt cagaagatcg aaactctcga
      121 atgctcgccg gagtttaacc ggcttaattt taagcccgtc acctataccg acgccaaagg
      181 cgaaaaacgc ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg tgatgggctt
      241 cactggcaaa aaagccgccg cattcaagga agcctacatt gctgagttcg accgcatgga
      301 ggccgaactg cgccagaata acaccccacc tgcagacaag atgattccgg gtgatggtcg
      361 cactctggtt gttcacttcg acaaattcgg caatatcaga ttcaccgaaa ctgttcctga
      421 cggtgcactg gtctgtaccc tggatacttt
//
LOCUS       DAJTRN010000369          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTRN010000369 DAJTRN010000000
VERSION     DAJTRN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..272
                     /locus_tag="OO328_004306"
     CDS             <1..272
                     /locus_tag="OO328_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR5932602.1"
                     /translation="YIEQVPRTGAKKLDTEQLNLLKKSQRAWLDFRDKECELILSNED
                     VQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLTRG"
BASE COUNT          139 a           92 c           82 g          134 t
ORIGIN      
        1 gttatataga acaagtgcca cgcacaggtg caaaaaaact tgatactgaa caacttaact
       61 tgcttaaaaa atcacagcga gcctggctgg attttaggga caaagaatgt gaattaatcc
      121 tttcaaatga ggatgttcag gatttaagtg acccctattc tgaatcagaa tggctatcat
      181 gtatgatcat acagaccaat acgcgaactc gccagttgca gctataccgt aactctgaag
      241 atttttatcc aagccctttg acaagaggat aattcacatc tttttggcat gttttgttgc
      301 aagctattcc tgataaatga ttgcaacaaa acatccagcc ttttgactga gttattaaac
      361 atactcgcga gcgcgtaatt ttttgtcttt cagcgataat tcacaatcac ttcattccgt
      421 tgtaccttta gcggtggaat taaccgc
//
LOCUS       DAJTRN010000370          446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTRN010000370 DAJTRN010000000
VERSION     DAJTRN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>446
                     /locus_tag="OO328_004307"
     CDS             <1..>446
                     /locus_tag="OO328_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5932603.1"
                     /translation="TLRPQHLIRSGSQIQLSRPSEINRLKALVAKLQRMQFGKSSEKL
                     RAKTERWIQEAQERISALQEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVI
                     RPEEECCPACGGELSPLGCDVSEQLELISSAFKVIEKQRPKLACRR"
BASE COUNT          111 a          127 c          122 g           86 t
ORIGIN      
        1 actttacgcc cacagcacct aatcaggtct gggtcgcaga ttcagttgtc caggccttcc
       61 gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc gtatgcagtt cggtaaaagc
      121 tcagaaaaac ttcgcgcaaa aaccgaacgg tggatacagg aagcacagga gcgaatcagc
      181 gcacttcagg aggaaatggc ggaaacactg ggtgagcaat atgacccggt actgccatct
      241 tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct cacttccccg tgccccccgg
      301 gtcatccggc cggaagagga atgctgtccg gcctgtggtg gtgaactcag tccactggga
      361 tgtgatgtgt cagagcagct ggagcttatc agcagcgcct ttaaggttat cgaaaaacaa
      421 cgccctaaac tggcctgtcg ccggtg
//
LOCUS       DAJTRN010000371          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTRN010000371 DAJTRN010000000
VERSION     DAJTRN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..76)
                     /locus_tag="OO328_004308"
     CDS             complement(<1..76)
                     /locus_tag="OO328_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5932604.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFF"
     gene            complement(51..239)
                     /locus_tag="OO328_004309"
     CDS             complement(51..239)
                     /locus_tag="OO328_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5932605.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(250..>442)
                     /gene="cspG"
                     /locus_tag="OO328_004310"
     CDS             complement(250..>442)
                     /gene="cspG"
                     /locus_tag="OO328_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5932606.1"
                     /translation="LVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNENQKV
                     EFSIEQGQRGPAAANVVTL"
BASE COUNT          133 a           94 c          106 g          109 t
ORIGIN      
        1 tgaagaaaaa taaggcagat ataaaaaagg ctccacgagg gagcctgata ttattttgtg
       61 aaatccagcg gcgcatggtc agtgcgtatt tgcagcgcga cgttatcaaa ggtgatcata
      121 gtcgatttac aaaaaataca cttagcgccc aaagggtttc gctccgtcac atcgaatgcc
      181 gatgtacggt actgtgaacc atgacaacat ggacagcgga aatggagatg ttgagtaata
      241 acggcaacct tagagcgtaa caacgttcgc tgccgcgggg ccacgttgcc cctgctcaat
      301 agaaaattca actttctgat tttcgttcag cgtgcggaat tcattgctct ggatggcggt
      361 gaaatggacg aaaacgtctt tgctgccatc atcaggagtg ataaagccaa aacctttatc
      421 tgcgttaaac cattttacta aa
//
LOCUS       DAJTRN010000372          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTRN010000372 DAJTRN010000000
VERSION     DAJTRN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..269
                     /locus_tag="OO328_004311"
     CDS             <1..269
                     /locus_tag="OO328_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5932607.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>439
                     /gene="tnpB"
                     /locus_tag="OO328_004312"
     CDS             266..>439
                     /gene="tnpB"
                     /locus_tag="OO328_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5932608.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILW"
BASE COUNT          109 a          114 c          125 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtgg
//
LOCUS       DAJTRN010000373          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTRN010000373 DAJTRN010000000
VERSION     DAJTRN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(241..>439)
                     /locus_tag="OO328_004313"
     CDS             complement(241..>439)
                     /locus_tag="OO328_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932609.1"
                     /translation="HGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGTISHMYMK
                     GGAIPWQRYPFVTMISVERKG"
BASE COUNT          119 a          108 c          107 g          105 t
ORIGIN      
        1 aggcagcggt gaacatagcc gtttgcaggt ggcattccag ggccagggaa tcaaatggct
       61 ggtggcagct tacgcccggc tggagtcggt gtaacgttgc cggatgcggt gctgcgcacc
      121 ttatttggcc taaaaaatca ttcagattca ataaattgca acgtcatgta ggccggataa
      181 ggcgtttacg ccgcatccgg catcagcagc atatttagta tctgacacaa agctatcatt
      241 ctaacctttc cgctcgacgg aaatcatggt gacaaaagga tagcgttgcc acgggattgc
      301 cccgcctttc atatacatat gtgaaatcgt gccaccaaga taaagcagct gctcaacgtt
      361 cagtttcgct ttggcataac aggcaaaatc ataaaaattt gtcgcctgct ggctcaacaa
      421 aaacacggca ttcccatgt
//
LOCUS       DAJTRN010000374          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTRN010000374 DAJTRN010000000
VERSION     DAJTRN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..279)
                     /locus_tag="OO328_004314"
     CDS             complement(1..279)
                     /locus_tag="OO328_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932610.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           95 a          107 c          135 g          102 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgcc
//
LOCUS       DAJTRN010000375          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTRN010000375 DAJTRN010000000
VERSION     DAJTRN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          102 a          117 c           89 g          129 t
ORIGIN      
        1 cgatactacc attggcgtta atttttcccg agcgtgtgac tgacaatgca gaagccgatg
       61 ttaccttact ggttgtttct gtaaaaacac acccaatgtc aactacctcg caattagctc
      121 ctgttatcca gtatggttgt tctgcacttg gctgataatc ccctgcgctc tggaacgttg
      181 tgccgttgag tatgactgta acttttcccc cacctgagct tgttacttga atattcgtgt
      241 gcgtgtatct tgcactgctc acgcgatgaa aatttccacc gtttataacc acagttacag
      301 ggcgcgtccc agtattatct atagcaaggt ctgccccccc ggcattagcc tcaaagtatg
      361 ggttgtttaa taccaaagcg cacgaaccgt taaggccatt tatagctaat tgcgcaccac
      421 ctgtactgga atccccc
//
LOCUS       DAJTRN010000376          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTRN010000376 DAJTRN010000000
VERSION     DAJTRN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            35..434
                     /locus_tag="OO328_004315"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO328_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTRN010000377          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTRN010000377 DAJTRN010000000
VERSION     DAJTRN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>434)
                     /gene="lysO"
                     /locus_tag="OO328_004316"
     CDS             complement(<1..>434)
                     /gene="lysO"
                     /locus_tag="OO328_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5932611.1"
                     /translation="ERGLPWRNHHQQEKLPSRIAMALESLKLCGVVVIGFAIGLSGLA
                     FLQHATEASEYTLILLLFLVGIQLRNNGMTLKQIVLNRRGMIVAVVVVVSSLIGGLIN
                     AFILDLPINTALAMASGFGWYSLSGILLTESFGPVIGSAAFF"
BASE COUNT          134 a          114 c           99 g           87 t
ORIGIN      
        1 ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt cggtcaataa aataccggaa
       61 agagaatacc agccgaaacc ggaggccatt gccagcgcgg tattgatggg gagatcaaga
      121 ataaaggcgt taattaaacc accaattaat gaactgacaa ccaccaccac ggcgacaatc
      181 attccccggc gattaaggac aatctgcttt aaggtcatgc cattattgcg caactgaata
      241 ccaacgagga aaagtagcaa aattaacgtg tattcactgg cttcggtcgc gtgttgtaag
      301 aaagccagtc cactcagacc aatggcaaaa ccaatcacta ctacgccgca cagttttagc
      361 gactccagcg ccatcgcaat acgcgacggg agtttttctt gctgatggtg gttgcgccac
      421 ggcaggcctc gctc
//
LOCUS       DAJTRN010000378          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTRN010000378 DAJTRN010000000
VERSION     DAJTRN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..163
                     /locus_tag="OO328_004317"
     CDS             <1..163
                     /locus_tag="OO328_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5932612.1"
                     /translation="NPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRRLSESV
                     PCMPHKKAG"
     gene            184..>433
                     /locus_tag="OO328_004318"
     CDS             184..>433
                     /locus_tag="OO328_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR5932613.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          142 a           97 c          101 g           93 t
ORIGIN      
        1 taatcctagt tttatcgcaa gccagatggg ccacacaaac gcacaaatgg tattcaatgt
       61 ttacggagca tggatgaaag acaacaatca cgaacagata gaacttctta acagaagact
      121 atctgaaagt gtcccatgca tgccccataa gaaagcgggg taaaataaaa acttgcaaaa
      181 tcaattggtt tacctttaat ccctgtcacg ttacgcgcgt ggcagaggcg ttacggattg
      241 ctgaaaccgc aacggacaga cggcggtcat cggctgttca acgatgccga tattgaccgg
      301 atccgcgaga tcaaacgctg gatcgacaac ggcgtgcagg tcagcaaagt taaaatactg
      361 ctcagtaatg aaaatgttga tgtgcagaac gtctggcgcg atcagcaaga aacattactg
      421 acttacctgc aaa
//
LOCUS       DAJTRN010000379          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTRN010000379 DAJTRN010000000
VERSION     DAJTRN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..310
                     /locus_tag="OO328_004319"
     CDS             <1..310
                     /locus_tag="OO328_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5932614.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
BASE COUNT          119 a          108 c           91 g          102 t
ORIGIN      
        1 actggacaat gtgttacagg agctggggcg cgaacgcacg attgctatga gcctgccgga
       61 attcgagcag tcactgttta tggcagcgca acccgacaat ctgctactgg cgaccgcgcc
      121 gcgctactgt cagtactaca atcaactcca tcaactgccg ttggttgctc ttcctctccc
      181 gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt ggcataaacg
      241 gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc tttacgcgtc
      301 gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataaaatg taaaaaagtt
      361 gtaataagct tgtctgaatc gaacttttag ccgctttagt ctgtccatca ttccagtaaa
//
LOCUS       DAJTRN010000380          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTRN010000380 DAJTRN010000000
VERSION     DAJTRN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>419
                     /locus_tag="OO328_004320"
     CDS             <1..>419
                     /locus_tag="OO328_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5932615.1"
                     /translation="NDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVV
                     MTAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPK
                     RTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAAS"
BASE COUNT           79 a           90 c          113 g          137 t
ORIGIN      
        1 taaatgatcc tgttccggcg ggtatcgcct gtattgctat cgtctgggta tttacctttg
       61 taaatatgct cggcggtacc tgggtaagcc gtttaaccac tattggtctg gtgctggttc
      121 ttattcctgt ggtgatgact gctattgttg gctggcattg gtttgatgcg gcaacttatg
      181 cagctaactg gaatactgcg gataccactg atggtcatgc gatcattaaa agtattctgc
      241 tctgcctgtg ggccttcgtg ggtgttgaat ccgcagcagt aagtactggt atggttaaaa
      301 acccgaaacg taccgttccg ctggcaacca tgctgggtac tggtttagca ggtattgttt
      361 atatcgctgc gactcaggtg ctttccggta tgtatccgtc ttctgtaatg gcggcttcc
//
LOCUS       DAJTRN010000381          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTRN010000381 DAJTRN010000000
VERSION     DAJTRN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(23..>418)
                     /locus_tag="OO328_004321"
     CDS             complement(23..>418)
                     /locus_tag="OO328_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932616.1"
                     /translation="NAAFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQV
                     IPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTG
                     RNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          110 a          109 c           84 g          115 t
ORIGIN      
        1 atatttttta aatgcaacat tgttaaatac tcgctacgtt ataccaggtg ccattaatca
       61 gtttttgcag tggtcggtaa tacacaccgt caacgttatc cgctgagtta cggccggtat
      121 ccgatatcgc catccctgac agcccgtgtc ccgaaggtgc gcaaaatctc cacgaaacgg
      181 tgttactgcc ggggctgtaa tacatttcat ggccataccg tacatcctgt accccctctg
      241 ttcgatattt atagcgggca tcgaagtttc cgtaatttaa cggaattacc tgtccgttaa
      301 cagtgaacac tatgctgtta tccgggtttc tctggctgaa aaaatgccag cctgaatcat
      361 caccaagctc tgcaacaaca ggcctggatg gattacccca caaataaaac gctgcatt
//
LOCUS       DAJTRN010000382          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTRN010000382 DAJTRN010000000
VERSION     DAJTRN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>418
                     /gene="curA"
                     /locus_tag="OO328_004322"
     CDS             <1..>418
                     /gene="curA"
                     /locus_tag="OO328_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5932617.1"
                     /translation="YLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDY
                     QPGDWVLGYSGWQDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPK
                     EGETLVVAAATEPVGATVGQIGKLKGCRVVGVAGGAE"
BASE COUNT           84 a           87 c          151 g           96 t
ORIGIN      
        1 tatttgtccc tggacccgta tatgcgtgga cgtatgagcg atgagccatc ttattcaccg
       61 ccggttgata ttggcggcgt gatggtcggc ggtacggtga gccgtgtcgt ggagtcgaat
      121 catcctgatt atcagcctgg cgactgggtg ctgggctaca gtggatggca ggactatgac
      181 atatccagtg gtgatgacct ggtgaaactt ggcgatcatc cgcaaaatcc atcgtggtcg
      241 ctgggtgtgc tggggatgcc aggctttacc gcttatatgg ggctgctgga tatcggtcag
      301 cctaaagagg gcgaaacgtt ggtggtagct gcggcgacag aaccagtggg ggcgacggtg
      361 gggcaaatcg gcaaacttaa aggttgcaga gtggtggggg tagccggtgg cgcggaaa
//
LOCUS       DAJTRN010000383          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTRN010000383 DAJTRN010000000
VERSION     DAJTRN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..394
                     /gene="ychE"
                     /locus_tag="OO328_004323"
     CDS             <1..394
                     /gene="ychE"
                     /locus_tag="OO328_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5932618.1"
                     /translation="LVVTIAMSMISGKLGEDKQNKQEKSETAVRESIGVVPLALPLMA
                     GPGAISSTIVWGTRYHSISYLFGFFVAIALFALCCWGLFRMAPWLVRVLRQTGINVIT
                     RIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT           89 a           81 c          125 g          122 t
ORIGIN      
        1 cctggtggtg acgatagcga tgtcgatgat cagcggcaag cttggcgagg ataaacagaa
       61 caagcaagaa aaatcagaaa ccgcggtacg tgaaagtatt ggtgtggtgc cattggcgtt
      121 gccgttgatg gcggggccag gggcgatcag ttctaccatc gtctggggta cgcgttatca
      181 cagcattagc tatctgtttg gtttctttgt ggctattgca ttgttcgctt tatgttgctg
      241 gggattgttc cgcatggcac cgtggctggt acgggtttta cgccagaccg gcatcaacgt
      301 gattacgcgt attatggggc tattgctgat ggcattgggg attgaattta tcgttactgg
      361 tattaagggg attttccccg gcctgcttaa ttaattcctt tcaaatgaaa cggagct
//
LOCUS       DAJTRN010000384          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTRN010000384 DAJTRN010000000
VERSION     DAJTRN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..233
                     /locus_tag="OO328_004324"
     CDS             <1..233
                     /locus_tag="OO328_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5932619.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(315..413)
                     /locus_tag="OO328_004325"
                     /pseudo
     CDS             complement(315..413)
                     /locus_tag="OO328_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT           97 a           88 c          109 g          119 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 gtcgtcagag ctgcgatata tctccttcct ccatgggctg tctcttatac acaaatcccc
      301 acccggggat ttgtgatgtc agatgtcccc agtaagacta tgttttcatt gcgctctatg
      361 aagctgagtg agcgtaacga ctggagctgc ttctgcggtg ctccggtggc gaa
//
LOCUS       DAJTRN010000385          412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTRN010000385 DAJTRN010000000
VERSION     DAJTRN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          143 a           68 c           59 g          142 t
ORIGIN      
        1 ccggaaagta tgcagaccat ctgccattat accgccaaag tgatctacta ttccacacag
       61 ctatttagct ctctttttgt tgagctccca tgattctttc atttattgtt actaattatt
      121 ttatgttatc atgatgttag aacacgctct gctctgtgtc tctattttat ctatcatatt
      181 ctgaggatta actacataac aaggcgcaag atatatatac aaaaacaaat aataccgttg
      241 caataattcc ttttagtaaa ggaggtgtga ttaatttcag gggatttcag agtaaaaatt
      301 gtacaacata ttaaaatatg ttcatttcgt tataatacac acaaggaaat attaagttag
      361 ataatatatt ctaaataacc atagataaaa acgcacataa tggggaatat tt
//
LOCUS       DAJTRN010000386          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTRN010000386 DAJTRN010000000
VERSION     DAJTRN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..260)
                     /locus_tag="OO328_004326"
     CDS             complement(<1..260)
                     /locus_tag="OO328_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5932620.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLR"
BASE COUNT          114 a           84 c           92 g          120 t
ORIGIN      
        1 cgcaattttt gcaggctctc tttcagactc gggatcaatt tgtctttgct gagattttca
       61 atccagattt tagtggtgat gtagagttca tgacgtggca cgccactttc tgcaatcgcc
      121 tgacctactg cggcttcgtt atcatagatt tgtgcggtat caattgcgcg ataaccaagt
      181 tcaagcgccg ttttcacaga tgaaataaca acgtcgtctt tcagacggaa agtacctaaa
      241 ccaaatgcag ggatagccat aatatgcctc ttttaatgct attttcgtta actgaggtga
      301 ttatgggctt cagactgttg cgggaaaaga gcgtaaaacg cagaggattt ttgcgattct
      361 agcaataata gatatccaga cgcaaaaaaa catcgagcgt taactcgagg
//
LOCUS       DAJTRN010000387          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTRN010000387 DAJTRN010000000
VERSION     DAJTRN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..315)
                     /locus_tag="OO328_004327"
     CDS             complement(<1..315)
                     /locus_tag="OO328_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5932621.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYL"
BASE COUNT          114 a           92 c          104 g           98 t
ORIGIN      
        1 caggtaaccg ctggttaaac gttcatagcg cccttcatct tttagcgcct gaatagaaag
       61 gggaccaaaa acttcttccg gcgtggagaa gcgggtcatc agatattcaa acgcgcgggc
      121 attctgaaag gcgaatttta agcgccgggc gatcaaactt ttggcaatac ctggcgggcc
      181 aaggaggaac acactttcac cacttaatgc cgctaataaa cacaagcgga tggcgtggct
      241 acgttcataa agcccctttt ccaacgaact gctcaggcgg gaaattcttt ccgctaataa
      301 atgagggtga gccataatga agtggcgtcc tttcgtcaaa agttctgcgt aaattgcgag
      361 tatagacgtt tcttgctggt gactaaaata gtctcaaagg ggggtaat
//
LOCUS       DAJTRN010000388          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTRN010000388 DAJTRN010000000
VERSION     DAJTRN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>408
                     /locus_tag="OO328_004328"
     CDS             <1..>408
                     /locus_tag="OO328_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5932622.1"
                     /translation="RHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALL
                     RAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLR
                     RSNIINLEWQQIDMQRRVAWINPEESKSNRAIG"
BASE COUNT          138 a           91 c          104 g           75 t
ORIGIN      
        1 ggcgtcatga ggaaaactgg agactcaggg cagaagcatg cagaaaaaaa gggaaacctg
       61 ttccagaata cacgccaaaa ccagcgtccg ttgcaacgaa ggctacgcat ctttcattta
      121 taaaggccct actaagagcc gcagagcgtg aatggaaaat gctggataag gcaccaatta
      181 ttaaagtgcc tcaaccaaag aataaacgga tccgctggct ggagccccat gaagcacaaa
      241 ggctgattga tgaatgtccg gagccattaa agtctgttgt tgaatttgca ctggcaacag
      301 gcttaagacg ctcgaacatc atcaaccttg aatggcaaca aatagatatg cagcgccggg
      361 tggcatggat aaacccggaa gagagtaaat caaaccgcgc aattggcg
//
LOCUS       DAJTRN010000389          399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTRN010000389 DAJTRN010000000
VERSION     DAJTRN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            135..>399
                     /locus_tag="OO328_004329"
     CDS             135..>399
                     /locus_tag="OO328_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932623.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGL"
BASE COUNT          109 a           97 c           94 g           99 t
ORIGIN      
        1 gctgttcttt gcctggttct gatacccgtg gaagtcgtta ccggaggaaa cacgttcacc
       61 ctactacctg agaacatctt cttcaaaaat tacggcaacg cttcttcctg tgttacagaa
      121 cacgaggaag aaacatgcaa acttataaca ccaatcccgc ttacgaaatg gctccccagc
      181 tcctggagca ctttaaccag catctggatt cattattcgg ggtctattcg aaactgttac
      241 cgttcagaat ggattttgct taccgaaaaa acaccctcag ctacagatgt gcctgcaggt
      301 acgccatgtg cgcggagatg ctgcaactga ttaacgaggt gggagagaaa ctggtcgggt
      361 atgcctgggt gatggaatat acagaacgta aagggcttc
//
LOCUS       DAJTRN010000390          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTRN010000390 DAJTRN010000000
VERSION     DAJTRN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(78..>397)
                     /locus_tag="OO328_004330"
     CDS             complement(78..>397)
                     /locus_tag="OO328_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5932624.1"
                     /translation="EIGKVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLG
                     ENVTVIVNGDITQCDLPRGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLH
                     AYS"
BASE COUNT          101 a          121 c           85 g           90 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccg
//
LOCUS       DAJTRN010000391          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTRN010000391 DAJTRN010000000
VERSION     DAJTRN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..230
                     /locus_tag="OO328_004331"
     CDS             <1..230
                     /locus_tag="OO328_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5932625.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT          105 a           88 c          113 g           89 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 acgtacttct cggggcgctt accatacggt ggtcggttta cgcttaccgc aggacaggtg
      301 gtaactgaaa aatagtttgc tgaggcgact ggaaacagaa gggcaagatc gattccgaca
      361 gcagtgatgg tcagctacct catcgataac tttgc
//
LOCUS       DAJTRN010000392          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTRN010000392 DAJTRN010000000
VERSION     DAJTRN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.402"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>387
                     /locus_tag="OO328_004332"
     CDS             <1..>387
                     /locus_tag="OO328_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5932626.1"
                     /translation="HRALSEHKTLSVSWSHYLNGKVIAPACTLVAATKDSVVTLPNVS
                     ATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAAT
                     NVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          110 a           90 c           86 g          101 t
ORIGIN      
        1 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta ccttaatggt
       61 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
      121 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
      181 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
      241 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
      301 gcgacgaatg ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt
      361 accgaaacgt cgaatattca gttagcg
//
LOCUS       DAJTRN010000393          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTRN010000393 DAJTRN010000000
VERSION     DAJTRN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>384)
                     /locus_tag="OO328_004333"
     CDS             complement(<1..>384)
                     /locus_tag="OO328_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932627.1"
                     /translation="MALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLED
                     YAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQLWSSEKYLDFT
                     PKLFEAVRNKFGFNEHLLHDMHHRLT"
BASE COUNT           94 a          110 c           82 g           98 t
ORIGIN      
        1 ggtcaggcga tggtgcatgt catgcaacag atgttcgtta aaaccgaact tattacgcac
       61 cgcttcgaat aacttcggcg taaaatcgag atacttttct gaagaccaca actgctcttc
      121 tggccacagg cctttggttg ccggttcata cgccagtcct ttgcccttcg ccaggccata
      181 agtggtttgc atacccggta caccgcactg cacacgaatc gccttaaagc ccatctgctg
      241 gtgctttgca tagtcttcaa ggacttcatc aatcgtacgc ccggtggtgt ggcaataaac
      301 catcacgcct tcacgagaag caccaccaag cagttgatac aatggcatat ttgccgcttt
      361 agctttgata tcccacaacg ccat
//
LOCUS       DAJTRN010000394          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTRN010000394 DAJTRN010000000
VERSION     DAJTRN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            171..379
                     /locus_tag="OO328_004334"
                     /pseudo
     CDS             171..379
                     /locus_tag="OO328_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           95 a           87 c          116 g           81 t
ORIGIN      
        1 ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg aaattctccg cgacagaaat
       61 cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt cttgcggcgc agtgatatcg
      121 caaggcacac cgagcaggga attctggtta tcggtggggc tgttcgccag aaaaaatttg
      181 acccgttgtc agttgatctg cccgactggt tgtcagaaac actctggcat gagtgggtcc
      241 agttcaggca ggcattgcga aaaccgattc gaacggagct gggcgctaac ggggcgatac
      301 gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt cgacacagca
      361 tcgccaatga ataccaggg
//
LOCUS       DAJTRN010000395          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTRN010000395 DAJTRN010000000
VERSION     DAJTRN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.405"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          130 a           78 c           66 g          103 t
ORIGIN      
        1 cagaatggcc tgacattatc ccgaaagtct ttccacttcc ctgtttctcc ggtaataaaa
       61 tattttaaat gctgaaggta taaagccata accagatatt gcaattaacg tagcattaca
      121 catatcaacg taccagataa gtaacagagg tacaggattg aacaacacgc cggaagactg
      181 tttaatgaca taaggtatac tcttttacga gtaagattat gtattaccac taatactcat
      241 caggtgagtt gcaggagcag attattaaca gaaatccttc aaacacagtt atccaggata
      301 ttaacacggg tgaagccata tagactggcc ccctgaatct ccagacaacc agtatcactt
      361 atttaagtga tagtctt
//
LOCUS       DAJTRN010000396          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTRN010000396 DAJTRN010000000
VERSION     DAJTRN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.406"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>374
                     /locus_tag="OO328_004335"
     CDS             <1..>374
                     /locus_tag="OO328_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5932628.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAJTRN010000397          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTRN010000397 DAJTRN010000000
VERSION     DAJTRN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.407"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          132 a           54 c           60 g          126 t
ORIGIN      
        1 ccgacaggtg agaattcaga atctataaag gggtatagcg aaaaaacatt gcattagaaa
       61 agtatgtttt cgaacattca ataaataaat agagatatgc atcacaaata caataacaaa
      121 caaaatgttg attttgtgat gaacataaca aatacaaggt tgactttaaa aatatcaatt
      181 tcatattgtt gttatttaag gttttattat tttggtttgt ggggtttttc ttctcgcgta
      241 cccaattgct ttcattaaac cgcacgagta ttttattttt accgccggac ggtaccctaa
      301 aaataaaata aggataattt atgcggctta gtgattattt tccagaatca tcaatctcag
      361 ttatacattc ag
//
LOCUS       DAJTRN010000398          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTRN010000398 DAJTRN010000000
VERSION     DAJTRN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.408"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..311)
                     /locus_tag="OO328_004336"
     CDS             complement(<1..311)
                     /locus_tag="OO328_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932629.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTRN010000399          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTRN010000399 DAJTRN010000000
VERSION     DAJTRN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.409"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(263..>371)
                     /locus_tag="OO328_004337"
     CDS             complement(263..>371)
                     /locus_tag="OO328_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5932630.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          103 a           84 c           75 g          109 t
ORIGIN      
        1 acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa ggaaccgtca
       61 gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag gcacagctat
      121 ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa ttcactgtca
      181 tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat cgacgacatt
      241 attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt gctccatttt
      301 ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca agcatagtgt
      361 ccccactatt t
//
LOCUS       DAJTRN010000400          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTRN010000400 DAJTRN010000000
VERSION     DAJTRN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.410"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>370
                     /locus_tag="OO328_004338"
     CDS             <1..>370
                     /locus_tag="OO328_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR5932631.1"
                     /translation="LPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQ
                     LTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAP
                     WSALCYLRIPGMEMLVKNYGI"
BASE COUNT           74 a           95 c           91 g          110 t
ORIGIN      
        1 ctgcccctga tgatgtgggg agcaatgcgc tatggttata agctgatttc gctgctctgg
       61 gcggtcgtgt tgatgatcag catccacagc tatcaaaatt acattcccat ttatcctggc
      121 tataccacgc agctgaccat aacctcctcc agttatctgg tattctcttt tattgtcaat
      181 tatatggctg tactggcaac tcgtcagcga gcggtagtca gacgcattca gcggcttgcg
      241 tatgtggacc cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc gttgcgtgat
      301 gccccctggt ctgcgctttg ttatttacgc atccctggca tggaaatgct ggttaagaac
      361 tatggcatca
//
LOCUS       DAJTRN010000401          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTRN010000401 DAJTRN010000000
VERSION     DAJTRN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.411"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            163..>370
                     /locus_tag="OO328_004339"
     CDS             163..>370
                     /locus_tag="OO328_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5932632.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMK"
BASE COUNT          115 a           78 c           91 g           86 t
ORIGIN      
        1 ttcagaatca tcttgctgct gcttcgattc agcaaggata aagggtatga tagtgaaaag
       61 ggataaaagc attgtcatct gcggcagcta tgagtaatgt tggccctaac gaatagcggt
      121 tgcttaaacg aatccgactc tcacattatc aggggtataa aaatggaaac taccaagcct
      181 tcattccagg acgtactgga atttgttcgt ctgttccgtc gtaagaacaa actgcaacgt
      241 gaaattcagg acgttgagaa aaagatccgt gacaaccaga agcgcgtcct gttgctggac
      301 aacctgagcg attacatcaa gccagggatg agcgttgaag caatccaggg catcatcgcc
      361 agcatgaaag
//
LOCUS       DAJTRN010000402          369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTRN010000402 DAJTRN010000000
VERSION     DAJTRN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.412"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..171
                     /locus_tag="OO328_004340"
     CDS             <1..171
                     /locus_tag="OO328_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932633.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..>369
                     /locus_tag="OO328_004341"
     CDS             276..>369
                     /locus_tag="OO328_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932634.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHEL"
BASE COUNT          113 a           62 c           83 g          111 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgc
//
LOCUS       DAJTRN010000403          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTRN010000403 DAJTRN010000000
VERSION     DAJTRN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.413"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>368
                     /locus_tag="OO328_004342"
     CDS             <1..>368
                     /locus_tag="OO328_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5932635.1"
                     /translation="SSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYKARL
                     KEAKLLASTGSTGDSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNR
                     RLLERLGHIPPAEAEKAYYA"
BASE COUNT          105 a           87 c          104 g           72 t
ORIGIN      
        1 ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt tgtgggcccg
       61 tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt cactggccta
      121 taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg gtgactcgta
      181 tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa tacaccgtaa
      241 gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg actggtataa
      301 caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag aaaaagctta
      361 ttatgctt
//
LOCUS       DAJTRN010000404          367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTRN010000404 DAJTRN010000000
VERSION     DAJTRN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.414"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(35..307)
                     /locus_tag="OO328_004343"
     CDS             complement(35..307)
                     /locus_tag="OO328_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932636.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           84 a           85 c           98 g          100 t
ORIGIN      
        1 tgtgaacagg cgtttcagcg gtcggggctg aaaatcacca ccacttcggg tcatccgcct
       61 tcatctccgc ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt
      121 attcgtacgg tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg
      181 agggatgacc ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg
      241 cgacggcttt cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt
      301 gtgacatggg aactcattct ggatggttac tctgaaagct catattctgc cacaccccga
      361 tttgcag
//
LOCUS       DAJTRN010000405          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTRN010000405 DAJTRN010000000
VERSION     DAJTRN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.415"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>365
                     /locus_tag="OO328_004344"
     CDS             <1..>365
                     /locus_tag="OO328_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5932637.1"
                     /translation="YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAA
                     GFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEY
                     AAIFVPGGHGALIGLPESQ"
BASE COUNT           97 a           85 c           88 g           95 t
ORIGIN      
        1 gtatcgcggt aaacataaaa ttctggtgat cgccgcggac gaacgttatt tgccgaccga
       61 taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc cgttgtatca
      121 tctccatgct gcaggtttcg aattcgaagt ggcgaccatt tccggtctga tgaccaagtt
      181 tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg aacagcataa
      241 atcgttattc cgtaatccga agaaactcgc ggatgttgtc gccagcctca acgctgatag
      301 cgaatatgca gcaatctttg ttcctggtgg gcatggcgca cttattggtt taccagaaag
      361 ccagg
//
LOCUS       DAJTRN010000406          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTRN010000406 DAJTRN010000000
VERSION     DAJTRN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.416"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>365
                     /locus_tag="OO328_004345"
     CDS             <1..>365
                     /locus_tag="OO328_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5932638.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAJTRN010000407          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTRN010000407 DAJTRN010000000
VERSION     DAJTRN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.417"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          117 a           71 c           53 g          120 t
ORIGIN      
        1 ccatgataat taacatggaa gacgtttcca tcgtattatt catttcatca ctgttttagc
       61 atcacgtttc aatagtatcc tttcataggg ctacccagga tgcggtcacc ttcattgtcg
      121 ctctaagcga ctagatcatc agaaatctcc agttagattg catacttcct tacacacttt
      181 cgtgaaactt cagggtaagg tgagaaagcc gtcatccctt tttctaattc cagcatctaa
      241 ataaattccg aattttgtta ttagtatcgc taccgcaata aaaacagata gtaaagagag
      301 taatatatac tttattgata tattaagaat aattaattgt taacatatct aatccaataa
      361 g
//
LOCUS       DAJTRN010000408          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTRN010000408 DAJTRN010000000
VERSION     DAJTRN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.418"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>360)
                     /locus_tag="OO328_004346"
     CDS             complement(<1..>360)
                     /locus_tag="OO328_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5932639.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVRGDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAP"
BASE COUNT           71 a           97 c          113 g           79 t
ORIGIN      
        1 agggcgcagt gacacgggcg aaagtcgcat cgtccggcgc ttcttttaat gcggcgggat
       61 ctggcgtcag cataatcagc aactgttcaa gaaacgccgt gccggtaaag tccggtgggc
      121 gcggataagt aaccgtgccg ggattggctt tagcaaactc cagtaaggct tgcggcgttt
      181 gtggtggctg tggcgtaaca tcgcggcggg cgataaacgt cagttgtgcg ccgccccagg
      241 gcgattccgc accttgtgtc ggcactgaaa aatctccccg caccggcagc tgtgtgtcga
      301 catagcgcca gttgggcaga gtctccgccc agcccgtttg taataaattg gcctctttta
//
LOCUS       DAJTRN010000409          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTRN010000409 DAJTRN010000000
VERSION     DAJTRN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.419"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..268
                     /locus_tag="OO328_004347"
     CDS             <1..268
                     /locus_tag="OO328_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932640.1"
                     /translation="KERISSLDVRDDIERRAYEIALLSLEVEPDEREAYELFMEKRFG
                     DLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            184..>360
                     /locus_tag="OO328_004348"
     CDS             184..>360
                     /locus_tag="OO328_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932641.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRL"
BASE COUNT          106 a           79 c           90 g           85 t
ORIGIN      
        1 taaagagcgt atcagcagcc ttgacgtgcg agacgatatt gagcgccgtg cttatgaaat
       61 cgcactccta tctctggaag tagaaccaga tgaacgcgaa gcttatgaat tattcatgga
      121 aaagcgtttt ggtgacttag tagatcgtcg gagagcaaaa aacggcgata acgaatacat
      181 ggcatgggat atgactctcg gttggatcgt ctggcagcaa cgagctggta tccatttctc
      241 aacaatgtca cagcaagagg tgaaataatg gagccataca gcctcacact cgatgaggcc
      301 tgtcattttc tcaagatatc cagaccgact gccattaact ggatacgcac agggcgtctt
//
LOCUS       DAJTRN010000410          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTRN010000410 DAJTRN010000000
VERSION     DAJTRN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.420"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(3..>359)
                     /locus_tag="OO328_004349"
     CDS             complement(3..>359)
                     /locus_tag="OO328_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5932642.1"
                     /translation="ASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVP
                     VVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERF
                     GFVQSVVAPNTLFYKV"
BASE COUNT          125 a           94 c           72 g           68 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagc
//
LOCUS       DAJTRN010000411          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTRN010000411 DAJTRN010000000
VERSION     DAJTRN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.421"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..107)
                     /locus_tag="OO328_004350"
     CDS             complement(<1..107)
                     /locus_tag="OO328_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5932643.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGD"
     gene            complement(248..>351)
                     /locus_tag="OO328_004351"
     CDS             complement(248..>351)
                     /locus_tag="OO328_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5932644.1"
                     /translation="CCSRVDVWLNLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT           97 a           98 c           90 g           66 t
ORIGIN      
        1 ttatcgccac cgacgacgcc aatcgaactg gtggcgaact gtgcagggtt ggcgtaaccg
       61 ggtacggaaa ccggcgagcc tttcggctcc cccacacagc ccgccataaa tcgcgaatgt
      121 gactgtgcaa acgatatgaa aaaagacgcg ggcgcgtctc atatcgctcc gtaaacatcc
      181 gggacgccaa tcccgacgcc agattttgct ggcgcgtgag gaatatagcc ccggataaca
      241 gattgagtca acaaacggtt tttagatccc cggaagagaa tgcatcacgc atcggtagat
      301 agagcatata ttcagcaaga tttagccata catctacccg actgcagcac g
//
LOCUS       DAJTRN010000412          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTRN010000412 DAJTRN010000000
VERSION     DAJTRN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.422"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>348
                     /gene="ynaI"
                     /locus_tag="OO328_004352"
     CDS             <1..>348
                     /gene="ynaI"
                     /locus_tag="OO328_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5932645.1"
                     /translation="KLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDI
                     TSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSG
                     IMLYFDRPFSIGD"
BASE COUNT           79 a           71 c           77 g          121 t
ORIGIN      
        1 ataaactcaa atttatcacc ccaggcgtta tcgattttat ctgtacgtcc ctgattgcgg
       61 ttattctgac gattaagcta tttctgctga ttaatcagtt tgaaaaacag cagattaaaa
      121 aaggtcgcga tatcaccagt gcgcggatta tgtcgcgcat tatcaaaatc accattattg
      181 tagtgcttgt tctgctttat ggcgaacatt tcggcatgag cctttctggc ttgctgacct
      241 ttggtggtat tggtggtctg gctgtcggta tggccggtaa agatattctg agtaatttct
      301 tttccgggat catgctctat ttcgaccgtc ctttcagtat tggcgact
//
LOCUS       DAJTRN010000413          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTRN010000413 DAJTRN010000000
VERSION     DAJTRN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.423"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..251
                     /locus_tag="OO328_004353"
     CDS             <1..251
                     /locus_tag="OO328_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HCR5932646.1"
                     /translation="VAEAIEAVAHGDESTSRVVGRVIDEIKLPPGTIIGAVVRGNDVM
                     IANDNLRIEQGDHVIMFLTDKKFITDVERLFQPSPFFL"
BASE COUNT          100 a           73 c           78 g           96 t
ORIGIN      
        1 gcgtagcaga agctattgaa gccgttgctc acggtgatga aagcacctca cgcgttgtcg
       61 gcagagtcat tgacgaaatc aagctaccgc caggaacgat tattggagcg gtggtacgtg
      121 gaaacgacgt gatgattgcc aatgacaatc tgcgcattga gcaaggcgat cacgtaatta
      181 tgttcctcac agataaaaag tttattaccg acgtcgaaag actcttccag ccaagccctt
      241 tcttcttgta attaataagg cgtctaatga cgccttatta tttccctttg attatcaagg
      301 attaattaaa ttcattcctg gcaggaaaat ggcttaacat ttgttag
//
LOCUS       DAJTRN010000414          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTRN010000414 DAJTRN010000000
VERSION     DAJTRN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.424"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>344)
                     /locus_tag="OO328_004354"
     CDS             complement(<1..>344)
                     /locus_tag="OO328_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5932647.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALT"
BASE COUNT          101 a           80 c           61 g          102 t
ORIGIN      
        1 ctgtcagtgc tcgcttaatc tcttcttcac ttcgccccat cagacgagaa tagccaatca
       61 tatttctaaa aagttcttca gaaccttgaa tgcctagaga ctctctagct gaagcaccga
      121 aaccagcaaa ctgtgattgc agttcagcga tatctaaacc cgttgtttga gctaatgact
      181 ttgtgaaatc ttttacttga tttgcatctt tagaaaatac gaaatcaatt gaacgttcag
      241 cacttgcacg ttgaaggcca acttccatta ctcgctgata agcggatagt acagcctgaa
      301 agctgataag tacacctgct gttttaactg ctgctgaact taaa
//
LOCUS       DAJTRN010000415          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTRN010000415 DAJTRN010000000
VERSION     DAJTRN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.425"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO328_004355"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO328_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5932648.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTRN010000416          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTRN010000416 DAJTRN010000000
VERSION     DAJTRN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.426"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(158..>342)
                     /locus_tag="OO328_004356"
     CDS             complement(158..>342)
                     /locus_tag="OO328_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5932649.1"
                     /translation="NSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASW
                     SALYVGSFSFVLPFFS"
BASE COUNT          112 a           71 c           81 g           78 t
ORIGIN      
        1 atcatcagcg accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt
       61 ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg
      121 ggggacgctg gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga
      181 agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat
      241 acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca
      301 cggcgataaa gcgcgaagtg aaaagtattg acacagagtt at
//
LOCUS       DAJTRN010000417          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTRN010000417 DAJTRN010000000
VERSION     DAJTRN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.427"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..90)
                     /locus_tag="OO328_004357"
     CDS             complement(<1..90)
                     /locus_tag="OO328_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5932650.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRM"
     gene            complement(87..>335)
                     /locus_tag="OO328_004358"
     CDS             complement(87..>335)
                     /locus_tag="OO328_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5932651.1"
                     /translation="VRIPGYCNHNPETSVLAHYRLAGTCGTATKPHDMQAAIACSSCH
                     DLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
BASE COUNT           67 a           86 c           79 g          103 t
ORIGIN      
        1 catgcgcagt tttccctgaa tccagacact ttccagcgtg gtgagacgaa ggtgttcccc
       61 gcttttgcct gtatttgttg ggtaaatcat aaataacctt cctttctcca gatttcttgt
      121 gtgcgaaaaa caccttctgc atgcatcagg cgtaattctt ctttggtgta atcgctggtt
      181 tttacccgcc cgtcgattaa atcgtggcat gagctacagg caatcgctgc ctgcatatcg
      241 tgtggttttg tcgctgttcc gcacgtcccc gccagcctgt aatgcgccag cacagaagtt
      301 tcgggattgt gattgcagta gccaggaatt ctgac
//
LOCUS       DAJTRN010000418          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTRN010000418 DAJTRN010000000
VERSION     DAJTRN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.428"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>334)
                     /locus_tag="OO328_004359"
     CDS             complement(<1..>334)
                     /locus_tag="OO328_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HCR5932652.1"
                     /translation="VIVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTM
                     ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLAR
                     LGRKSLSFS"
BASE COUNT           71 a          110 c           81 g           72 t
ORIGIN      
        1 ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat
       61 gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg cagctttccc ttcaggcggg
      121 attcatacag cggccagcca tccgtcatcc atatcaccac gtcaaagggt gacagcaggc
      181 tcataagacg ccccagcgtc gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct
      241 tccggagcct gtcatacgcg taaaacagcc agcgctggcg cgatttagcc ccgacgtatc
      301 cccactgttc gtccatttcc gcgcagacga tgac
//
LOCUS       DAJTRN010000419          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTRN010000419 DAJTRN010000000
VERSION     DAJTRN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.429"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            39..>332
                     /locus_tag="OO328_004360"
     CDS             39..>332
                     /locus_tag="OO328_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HCR5932653.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIELGYKSFNLEAV"
BASE COUNT           89 a           71 c           81 g           91 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattgagc
      301 ttggatataa atcttttaac ctcgaggccg tg
//
LOCUS       DAJTRN010000420          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTRN010000420 DAJTRN010000000
VERSION     DAJTRN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.430"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          107 a           49 c           83 g           91 t
ORIGIN      
        1 gtatcagacg tcttctgcgg tttctatgat agagcaattc tgtggcggat attaagataa
       61 cgggtatctg ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt
      121 gaaaaaatac acttgcctca atactggata tttaaacagg tctggagatg ctgtcacgat
      181 tagggtgcgg aaaaaggaag cgtaaataag aagaatcaaa agtacgacgg caatgggttg
      241 attgacaagg gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta
      301 ctctgaataa atgggagggt gacttgcctc
//
LOCUS       DAJTRN010000421          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTRN010000421 DAJTRN010000000
VERSION     DAJTRN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.431"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(50..190)
                     /locus_tag="OO328_004361"
     CDS             complement(50..190)
                     /locus_tag="OO328_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932654.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
BASE COUNT          118 a           46 c           46 g          119 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgcta
//
LOCUS       DAJTRN010000422          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTRN010000422 DAJTRN010000000
VERSION     DAJTRN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.432"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          143 a           47 c           61 g           78 t
ORIGIN      
        1 taaagatgat gacgggatca gaacagttat taatccatac aacaaaatac agaaaacaac
       61 agaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaataacaga cattacttaa cgaagaacat
      121 tacagtcagt gtgtacatta cagatttata ttcaggtgtt gcgggactac attacagcag
      181 agaggggtaa ggacagagtt acaagtacta ctagagagaa tatcactgca cagatacttc
      241 tatgagtata tctttatgag agatccgtct acaggattgg gttataaggt gtatttacaa
      301 ggtataatca caggatcatt tacatgacc
//
LOCUS       DAJTRN010000423          328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTRN010000423 DAJTRN010000000
VERSION     DAJTRN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.433"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          103 a           82 c           37 g          106 t
ORIGIN      
        1 cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta tatccgtgat
       61 gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt cagaaaacaa
      121 acttattttt attaaaatca attctttttc tttgaacatt caactgcgat atttgtaaaa
      181 acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat tccccgcata
      241 tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc aatacgcacg
      301 cgctacatta ctgtcaaatc gtggaaga
//
LOCUS       DAJTRN010000424          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTRN010000424 DAJTRN010000000
VERSION     DAJTRN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.434"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
BASE COUNT          117 a           60 c           42 g          106 t
ORIGIN      
        1 tggcaggttt ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg
       61 cgaaaaatta atgtaacgca gataaacaca tcccgattga attattcata agactattca
      121 caagcattat gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta
      181 atgaaaaatc acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca
      241 aaaaatataa catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac
      301 acggagttca acaatgggta atgct
//
LOCUS       DAJTRN010000425          324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTRN010000425 DAJTRN010000000
VERSION     DAJTRN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.435"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>324
                     /locus_tag="OO328_004362"
     CDS             <1..>324
                     /locus_tag="OO328_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5932655.1"
                     /translation="PQIREAAFRLLCLNHTFTSYISALGAHREQLTNPEILAFLDDAV
                     CYVDDALHHQPADEERVNQALAGLKQRMQQLEPRADSKEPLVVQQVGLLIALLPEIGR
                     LQRQI"
BASE COUNT           81 a           83 c           77 g           83 t
ORIGIN      
        1 ccccgcaaat tcgcgaagcc gcgtttcgct tgctgtgcct taaccatacg tttaccagct
       61 atatctcagc cctcggtgct caccgggagc agttaactaa tcctgaaatt ctggcgtttc
      121 ttgatgacgc agtttgctat gttgatgacg cgttacatca tcaacctgct gatgaagaac
      181 gcgtcaatca ggcattagct ggcctgaaac agcggatgca gcaacttgaa ccacgggcag
      241 atagcaaaga acctctggtc gtacaacaag ttgggttatt gattgcatta ctgccagaga
      301 ttggtcgtct gcaacgccag atta
//
LOCUS       DAJTRN010000426          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTRN010000426 DAJTRN010000000
VERSION     DAJTRN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.436"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..>318
                     /gene="csgA"
                     /locus_tag="OO328_004363"
     CDS             <1..>318
                     /gene="csgA"
                     /locus_tag="OO328_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5932656.1"
                     /translation="NSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADV
                     GQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQ
                     VGF"
BASE COUNT           79 a           78 c           83 g           78 t
ORIGIN      
        1 ataatagcgg cccaaattca gagctgaaca tttaccagta cggtggcggt aactctgcac
       61 ttgctctgca aactgatgcc cgtaactctg acttgactat tacccagcat ggcggtggta
      121 atggtgcaga tgttggtcag ggctcagatg acagctcaat cgatctgacc caacgtggct
      181 tcggtaacag cgccactctt gatcagtgga acggcaaaaa ttctgaaatg actgttaaac
      241 agttcggtgg tggcaacggt gctgcagttg accagactgc atcgaactcc tccgtcaacg
      301 tgactcaggt tggctttg
//
LOCUS       DAJTRN010000427          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTRN010000427 DAJTRN010000000
VERSION     DAJTRN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.437"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..72)
                     /locus_tag="OO328_004364"
     CDS             complement(<1..72)
                     /locus_tag="OO328_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5932657.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEF"
BASE COUNT          102 a           57 c           68 g           89 t
ORIGIN      
        1 gaactcggtt tgattcgggt cgcgcttttg gacatggttg aggaatgact ccagagaata
       61 tgtctgatcc atagatataa aaaccttata tattaatacg attgtgctta tgttgtttat
      121 gtcatgtgct tttgcagttt tcgactatac catcaagaat gtgacggtaa gcaagaaaaa
      181 aatgacccag gaaagcaaat taataacgag agtaatctca taaccaaatg cgataactca
      241 gacgctaaag ttttgccacc cggctaccgt tatcagtagc agagacaata acgttaagga
      301 gttccctgtg agtcga
//
LOCUS       DAJTRN010000428          308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTRN010000428 DAJTRN010000000
VERSION     DAJTRN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.438"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>308)
                     /locus_tag="OO328_004365"
     CDS             complement(<1..>308)
                     /locus_tag="OO328_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932658.1"
                     /translation="KDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKNTDAV
                     LPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKTATFD
                     "
BASE COUNT           78 a           63 c           83 g           84 t
ORIGIN      
        1 gtcaaatgtg gctgttttat tatcccgttg cgacacgcta caggctaagc gacgtaattg
       61 ggtcaatttg tctgcttgct gaacaggaac tccgggaaag cggcgatgac ttaacgtggt
      121 gtgtacaact tcgggcggaa aaacgacagc aatatctttc gccagccaag gtaaaacagc
      181 atctgtattt ttgtcagccg cacgggtttc tgctacgaaa gagggttgtt cctgaataat
      241 ggtggataag tccgctacct tgagagagtc cgttaccgtt tgggaaggtt gcaaagaatt
      301 atctttcg
//
LOCUS       DAJTRN010000429          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTRN010000429 DAJTRN010000000
VERSION     DAJTRN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.439"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>300)
                     /gene="arsR"
                     /locus_tag="OO328_004366"
     CDS             complement(<1..>300)
                     /gene="arsR"
                     /locus_tag="OO328_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5932659.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLAR"
BASE COUNT           73 a           78 c           77 g           72 t
ORIGIN      
        1 tctggccagg ttgcggacaa ttgcctgaat cttttcctgt tcacatcgcc aggcttcatc
       61 aataattttc gccgcccatg ctggaatatg cggcgataag cggtaatgaa cccacttacc
      121 ttgcttgcgg tccagcaata gcccgctttc acgcagcaat gccagatggc gggatatctt
      181 gggctgcgac tgatcgaggg cagtgcagag atcgcagacg cataactctc ccagttcgct
      241 aagcagtaaa acgatgccca gacgggtttc atcagcaaga attttgaaca attggatggg
//
LOCUS       DAJTRN010000430          297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTRN010000430 DAJTRN010000000
VERSION     DAJTRN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.440"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..>297)
                     /locus_tag="OO328_004367"
     CDS             complement(<1..>297)
                     /locus_tag="OO328_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5932660.1"
                     /translation="FSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIKIALP"
BASE COUNT           95 a           72 c           67 g           63 t
ORIGIN      
        1 tatggcagtg ctattttgat tcgacgttga tcaagtttct gataattcca acgacggtcg
       61 acgggcacca tccagtcaat caacatacct accgccagcc ctgtgatgac atcaataaaa
      121 tgatgctgcc atgtcgtcag cgtcgaaatg gcgatgagta aaaaccatcc gccacagact
      181 ttacgccacc tcacagccag atgctgacga aagtgacgcc aaagtagcca gcagagaata
      241 atatgcagcg aaggagactg gttataaggc agatcaaaca gttcaagttg cgaaaat
//
LOCUS       DAJTRN010000431          296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTRN010000431 DAJTRN010000000
VERSION     DAJTRN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.denovo.441"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(1..72)
                     /locus_tag="OO328_004368"
     tRNA            complement(1..72)
                     /locus_tag="OO328_004368"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Gln,seq:ttg)
     gene            complement(107..181)
                     /locus_tag="OO328_004369"
     tRNA            complement(107..181)
                     /locus_tag="OO328_004369"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:Gln,seq:ttg)
     gene            complement(205..289)
                     /locus_tag="OO328_004370"
     tRNA            complement(205..289)
                     /locus_tag="OO328_004370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Leu,seq:tag)
BASE COUNT           73 a           84 c           81 g           58 t
ORIGIN      
        1 ctggggtacc tggattcgaa ccagggaatg ccggtatcaa aaaccggtgc cttaccgctt
       61 ggcgataccc caataaccgg gcggtgaacc gcttactcga agaagatggc tggggtacct
      121 ggattcgaac cagggaatgc cggtatcaaa aaccggtgcc ttaccgcttg gcgatacccc
      181 atccgtacaa cgctttctgg tgaatggtgc gggaggcgag acttgaactc gcacaccttg
      241 cggcgccaga acctaaatct ggtgcgtcta ccaatttcgc cactcccgca aaaaag
//
LOCUS       DAJTRN010000432         5717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.168, whole genome shotgun sequence.
ACCESSION   DAJTRN010000432 DAJTRN010000000
VERSION     DAJTRN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.168"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO328_004371"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO328_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5932661.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO328_004372"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO328_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5932662.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO328_004373"
     CDS             2399..3235
                     /locus_tag="OO328_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5932663.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO328_004374"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO328_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO328_004375"
     CDS             complement(3790..4179)
                     /locus_tag="OO328_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5932664.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO328_004376"
     CDS             4397..4837
                     /locus_tag="OO328_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5932665.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1394 a         1516 c         1479 g         1328 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggtttgttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     5641 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     5701 aatccagttg ttcatca
//
LOCUS       DAJTRN010000433         4185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.194, whole genome shotgun sequence.
ACCESSION   DAJTRN010000433 DAJTRN010000000
VERSION     DAJTRN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.194"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            24..383
                     /gene="frdD"
                     /locus_tag="OO328_004377"
     CDS             24..383
                     /gene="frdD"
                     /locus_tag="OO328_004377"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5932666.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            446..1579
                     /locus_tag="OO328_004378"
     CDS             446..1579
                     /locus_tag="OO328_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5932667.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1668..2201
                     /gene="blc"
                     /locus_tag="OO328_004379"
     CDS             1668..2201
                     /gene="blc"
                     /locus_tag="OO328_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5932668.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2198..2515)
                     /gene="sugE"
                     /locus_tag="OO328_004380"
     CDS             complement(2198..2515)
                     /gene="sugE"
                     /locus_tag="OO328_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5932669.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2691..2837)
                     /gene="ecnB"
                     /locus_tag="OO328_004381"
     CDS             complement(2691..2837)
                     /gene="ecnB"
                     /locus_tag="OO328_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5932670.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2948..3073)
                     /gene="ecnA"
                     /locus_tag="OO328_004382"
     CDS             complement(2948..3073)
                     /gene="ecnA"
                     /locus_tag="OO328_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5932671.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(3125..3691)
                     /gene="efp"
                     /locus_tag="OO328_004383"
     CDS             complement(3125..3691)
                     /gene="efp"
                     /locus_tag="OO328_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5932672.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3733..>4185
                     /locus_tag="OO328_004384"
     CDS             3733..>4185
                     /locus_tag="OO328_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HCR5932673.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYV"
BASE COUNT         1041 a         1077 c         1052 g         1015 t
ORIGIN      
        1 cctgtactgg taaggagcct gagatgatta atccaaatcc aaagcgttct gacgaaccgg
       61 tattctgggg cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga
      121 tcctgctggt gggtattctg ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg
      181 agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg
      241 ttctgccgct gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaatcc
      301 acgtgcctgc gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc
      361 tgattggtgt cgttacaatc taacgcatcg ccaatgtaaa tccggcccgc ctatggcggg
      421 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
      481 cgcctcttac tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      541 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      601 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      661 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      721 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      781 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      841 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      901 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      961 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
     1021 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
     1081 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
     1141 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
     1201 caatttaccc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
     1261 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
     1321 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
     1381 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1441 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1501 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1561 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1621 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1681 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagtttt cctacgccgc
     1741 cgcgtggcgt gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg
     1801 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1861 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     1921 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     1981 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     2041 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     2101 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     2161 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     2221 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
     2281 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     2341 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     2401 tcgccgtcac ggtaataaca ctcggcgtca aacggctaaa gccgtgggta tatttcaggc
     2461 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2521 gctccggaaa tttggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2581 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttatga
     2641 cgatcgcaga aaaaacaaac ggcacgacac tgctgtcgta ccgttattgc ttattgctgc
     2701 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     2761 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     2821 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
     2881 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga
     2941 agaaaaatta gctggcagcg cgagagatgg agttaccgag atgtttgatg tcttcgccga
     3001 aaccgcaagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa
     3061 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
     3121 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
     3181 cgatttgtac gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc
     3241 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa
     3301 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg
     3361 cctgatccag cagccattta gcattgtcgc caattgcttt tgcatcagca gacagctgct
     3421 cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt
     3481 tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc
     3541 gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt
     3601 ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag
     3661 cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag
     3721 ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta
     3781 acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac
     3841 gcggacgaaa aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc
     3901 cgctcattta tcgatcgcat ggagaaaggc aatccggacg atcctctttt gcgtcaggta
     3961 cttacctcgc aagatgagtt tgtcgtcgcg tccggattct ccaccgaccc actggaagaa
     4021 cagcacagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc
     4081 aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa
     4141 aatcagggca acaagcgtaa ctggcagact gcacttgagt atgtt
//
LOCUS       DAJTRN010000434         3747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.200, whole genome shotgun sequence.
ACCESSION   DAJTRN010000434 DAJTRN010000000
VERSION     DAJTRN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.200"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            558..797
                     /locus_tag="OO328_004385"
     CDS             558..797
                     /locus_tag="OO328_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5932674.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO328_004386"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO328_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /locus_tag="OO328_004387"
     CDS             2294..2767
                     /locus_tag="OO328_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5932675.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="OO328_004388"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="OO328_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5932676.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..>3747
                     /locus_tag="OO328_004389"
     CDS             3444..>3747
                     /locus_tag="OO328_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR5932677.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRY"
BASE COUNT         1067 a          806 c          848 g         1026 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatc
//
LOCUS       DAJTRN010000435         3241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.210, whole genome shotgun sequence.
ACCESSION   DAJTRN010000435 DAJTRN010000000
VERSION     DAJTRN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.210"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            complement(<1..346)
                     /locus_tag="OO328_004390"
     CDS             complement(<1..346)
                     /locus_tag="OO328_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5932678.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFA"
     gene            complement(472..822)
                     /locus_tag="OO328_004391"
     CDS             complement(472..822)
                     /locus_tag="OO328_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5932679.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1025..2038)
                     /gene="intI1"
                     /locus_tag="OO328_004392"
     CDS             complement(1025..2038)
                     /gene="intI1"
                     /locus_tag="OO328_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5932680.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2248..3078
                     /locus_tag="OO328_004393"
     CDS             2248..3078
                     /locus_tag="OO328_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5932681.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          851 a          865 c          825 g          700 t
ORIGIN      
        1 cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct tcgccagtaa
       61 aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc gtttgcacga
      121 tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc accacggtgt
      181 cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca tccttgccgg
      241 atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt tccgggttct
      301 ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa ttccctgcaa
      361 aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca aaattcaatc
      421 ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc ctcaacctga
      481 cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc tggcccgcgc
      541 ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg ccccttccgg
      601 cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg tgctcggttc
      661 attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg ccgccgccgg
      721 catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc gcaaccacgc
      781 aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt tcagaagacg
      841 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atccatcagg
      901 caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc aaccggccgt
      961 tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat tgacttgcgc
     1021 tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc actccggcac
     1081 cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa tggccgagca
     1141 gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg aacgagtggc
     1201 ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca cgtttgaagg
     1261 cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc cgtggatcgg
     1321 tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg gcgcgcggat
     1381 acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc tggtccttca
     1441 gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag ctctcgggta
     1501 acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga
     1561 aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc gttccataca
     1621 gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat
     1681 ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc
     1741 agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac
     1801 gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt
     1861 cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     1921 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     1981 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2041 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2101 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2161 gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc cgttaaaatt
     2221 aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa cttcgctatt
     2281 tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga tatctctact
     2341 gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga tgcatccaca
     2401 aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc accagattca
     2461 actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga tcagaaaacc
     2521 atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa tcatacacca
     2581 aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca aaaaattgga
     2641 ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga cttctctgga
     2701 gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt aaaaatttca
     2761 ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc agttaaaaac
     2821 tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa tagtacaaaa
     2881 ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa cggatggttt
     2941 gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc acttacagga
     3001 aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat caccattcta
     3061 aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca atcaagtcgg
     3121 gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct tatttcaaac
     3181 gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3241 t
//
LOCUS       DAJTRN010000436         3046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.218, whole genome shotgun sequence.
ACCESSION   DAJTRN010000436 DAJTRN010000000
VERSION     DAJTRN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.218"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            263..922
                     /locus_tag="OO328_004394"
     CDS             263..922
                     /locus_tag="OO328_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5932682.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO328_004395"
     CDS             complement(1123..1500)
                     /locus_tag="OO328_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5932683.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>3046)
                     /locus_tag="OO328_004396"
     CDS             complement(1567..>3046)
                     /locus_tag="OO328_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5932684.1"
                     /translation="SLKQSSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAANDLP
                     DAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHFTHL
                     KSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALA
                     ELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTHISD
                     QYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRF
                     APRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTA
                     SLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNAL
                     ARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDD
                     SLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          668 a          784 c          867 g          727 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctg
//
LOCUS       DAJTRN010000437         2488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.230, whole genome shotgun sequence.
ACCESSION   DAJTRN010000437 DAJTRN010000000
VERSION     DAJTRN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.230"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            31..1236
                     /gene="tet(B)"
                     /locus_tag="OO328_004397"
     CDS             31..1236
                     /gene="tet(B)"
                     /locus_tag="OO328_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5932685.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1349..1942)
                     /gene="tetC"
                     /locus_tag="OO328_004398"
     CDS             complement(1349..1942)
                     /gene="tetC"
                     /locus_tag="OO328_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5932686.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2075..2446
                     /locus_tag="OO328_004399"
     CDS             2075..2446
                     /locus_tag="OO328_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932687.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          674 a          431 c          529 g          854 t
ORIGIN      
        1 cactccctat cagtgataga gaaaagtgaa atgaatagtt cgacaaagat cgcattggta
       61 attacgttac tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta
      121 cgtgaattta ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat
      181 gcgttaatgc aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg
      241 cgcccagtgc tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt
      301 tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact
      361 ggggctgtcg cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg
      421 ttcggttggt taggggcaag ttttgggctt ggtttaatag cggggcctat tattggtggt
      481 tttgcaggag agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc
      541 gctttccttg tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat
      601 accgaagtag gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg
      661 cccattttgt tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg
      721 tgggtgctat ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta
      781 gcgggtcttg gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact
      841 aaatggggcg aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc
      901 tttttagcgt ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt
      961 ggtgggatcg ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag
     1021 caaggtgctt tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca
     1081 ttactgtttg ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt
     1141 attggtttag cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct
     1201 caagctcagg ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta
     1261 ttccgcgaaa tataatgacc ctcttgataa cccaagaggg cattttttac gataaagaag
     1321 atttagcttc aaataaaacc tatctatttt atttatcttt caagctcaat aaaaagccgc
     1381 ggtaaatagc aataaattgg ccttttttat cggcaagctc ttttaggttt ttcgcatgta
     1441 ttgcgatatg cataaaccag ccattgagta agtttttaag cacatcatca tcataagctt
     1501 taagttggtt ctcttggatc aatttgctga caatggcgtt taccttacca gtaatgtatt
     1561 caaggctaat tttttcaagt tcattccaac caatgatagg catcacttct tggataggga
     1621 taaggttttt attattatca ataatataat caagataatg ttcaaatata ctttctaagg
     1681 cagaccaacc atttgttaaa tcagtttttg ttgtgatgta ggcatcaatc ataattaatt
     1741 gctgcttata acaggcactg agtaattgtt ttttattttt aaagtgatga taaaaggcac
     1801 ctttggtcac caacgctttt cccgagatct catctattga aacagcttga tagccttttt
     1861 caacaaacaa tattcgtgct gagttaacca gtgattgata ggtactctta aaattttctt
     1921 gttgatgatt tttattttcc atgatagatt taaaataaca taccgtcagt atgtttatgg
     1981 tatcatgatg atgtggtcgt gacaatctta agaacattta ggttatttta tgtatattga
     2041 acagcattct cgctatcaaa ataaagctaa taacatccaa ttaagatatg atgataagca
     2101 gtttcataca acggttatca aagatgttct attatggatt gaacataatt tagatcagtc
     2161 tttactgctt gatgatgtgg cgaataaagc gggttatacc aagtggtatt ttcagcggct
     2221 gttcaaaaaa gtaacagggg tcacactggc tagctatatt cgtgctcgtc gtttgacgaa
     2281 agcggctgtt gagttgaggt tgacgaaaaa aactatcctt gagatcgcat taaaatatca
     2341 atttgattcc caacaatctt ttacacgtcg atttaagtac atttttaagg ttacaccaag
     2401 ttattatcgg cgtaataaat tatgggaatt ggaggcaatg cactgagagg gtgatgctgc
     2461 caacttactg atttagtgta tgatggtg
//
LOCUS       DAJTRN010000438         2488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.231, whole genome shotgun sequence.
ACCESSION   DAJTRN010000438 DAJTRN010000000
VERSION     DAJTRN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.231"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            31..1236
                     /gene="tet(B)"
                     /locus_tag="OO328_004400"
     CDS             31..1236
                     /gene="tet(B)"
                     /locus_tag="OO328_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5932688.1"
                     /translation="MNSLTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1349..1942)
                     /gene="tetC"
                     /locus_tag="OO328_004401"
     CDS             complement(1349..1942)
                     /gene="tetC"
                     /locus_tag="OO328_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5932689.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2075..2446
                     /locus_tag="OO328_004402"
     CDS             2075..2446
                     /locus_tag="OO328_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5932690.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          674 a          430 c          529 g          855 t
ORIGIN      
        1 cactccctat cagtgataga gaaaagtgaa atgaatagtt tgacaaagat cgcattggta
       61 attacgttac tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta
      121 cgtgaattta ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat
      181 gcgttaatgc aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg
      241 cgcccagtgc tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt
      301 tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact
      361 ggggctgtcg cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg
      421 ttcggttggt taggggcaag ttttgggctt ggtttaatag cggggcctat tattggtggt
      481 tttgcaggag agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc
      541 gctttccttg tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat
      601 accgaagtag gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg
      661 cccattttgt tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg
      721 tgggtgctat ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta
      781 gcgggtcttg gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact
      841 aaatggggcg aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc
      901 tttttagcgt ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt
      961 ggtgggatcg ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag
     1021 caaggtgctt tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca
     1081 ttactgtttg ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt
     1141 attggtttag cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct
     1201 caagctcagg ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta
     1261 ttccgcgaaa tataatgacc ctcttgataa cccaagaggg cattttttac gataaagaag
     1321 atttagcttc aaataaaacc tatctatttt atttatcttt caagctcaat aaaaagccgc
     1381 ggtaaatagc aataaattgg ccttttttat cggcaagctc ttttaggttt ttcgcatgta
     1441 ttgcgatatg cataaaccag ccattgagta agtttttaag cacatcatca tcataagctt
     1501 taagttggtt ctcttggatc aatttgctga caatggcgtt taccttacca gtaatgtatt
     1561 caaggctaat tttttcaagt tcattccaac caatgatagg catcacttct tggataggga
     1621 taaggttttt attattatca ataatataat caagataatg ttcaaatata ctttctaagg
     1681 cagaccaacc atttgttaaa tcagtttttg ttgtgatgta ggcatcaatc ataattaatt
     1741 gctgcttata acaggcactg agtaattgtt ttttattttt aaagtgatga taaaaggcac
     1801 ctttggtcac caacgctttt cccgagatct catctattga aacagcttga tagccttttt
     1861 caacaaacaa tattcgtgct gagttaacca gtgattgata ggtactctta aaattttctt
     1921 gttgatgatt tttattttcc atgatagatt taaaataaca taccgtcagt atgtttatgg
     1981 tatcatgatg atgtggtcgt gacaatctta agaacattta ggttatttta tgtatattga
     2041 acagcattct cgctatcaaa ataaagctaa taacatccaa ttaagatatg atgataagca
     2101 gtttcataca acggttatca aagatgttct attatggatt gaacataatt tagatcagtc
     2161 tttactgctt gatgatgtgg cgaataaagc gggttatacc aagtggtatt ttcagcggct
     2221 gttcaaaaaa gtaacagggg tcacactggc tagctatatt cgtgctcgtc gtttgacgaa
     2281 agcggctgtt gagttgaggt tgacgaaaaa aactatcctt gagatcgcat taaaatatca
     2341 atttgattcc caacaatctt ttacacgtcg atttaagtac atttttaagg ttacaccaag
     2401 ttattatcgg cgtaataaat tatgggaatt ggaggcaatg cactgagagg gtgatgctgc
     2461 caacttactg atttagtgta tgatggtg
//
LOCUS       DAJTRN010000439         1775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.256, whole genome shotgun sequence.
ACCESSION   DAJTRN010000439 DAJTRN010000000
VERSION     DAJTRN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.256"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            174..632
                     /gene="gpt"
                     /locus_tag="OO328_004403"
     CDS             174..632
                     /gene="gpt"
                     /locus_tag="OO328_004403"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5932691.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            724..>1775
                     /gene="frsA"
                     /locus_tag="OO328_004404"
     CDS             724..>1775
                     /gene="frsA"
                     /locus_tag="OO328_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5932692.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLG"
BASE COUNT          399 a          450 c          481 g          445 t
ORIGIN      
        1 gatatgtatt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
       61 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
      121 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
      181 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
      241 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg
      301 gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
      361 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
      421 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgctgttgcg attcgtgaaa
      481 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
      541 atgactatgt tgttgatatc ccgcaagata cctggatcga acagccgtgg gatatgggcg
      601 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ttgccgggcg
      661 ttgttctttt taacttcagg caggttacaa tagtttccag taagtattct ggaggctgca
      721 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
      781 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt
      841 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
      901 ggtattgacc cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa
      961 cgtaccgacg atgatttata cgataccgtg attggctacc gtggtggcaa ctggatttat
     1021 gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct gtacggaaga agatccgcaa
     1081 ctcagtggtc gacactggct gcatgcggct acgttgtaca acattgccgc ctatcctcat
     1141 ctgaaaggag atgacctggc cgagcaagcg caggctttat caaaccgcgc ctatgaagag
     1201 gccgcccagc gtctgccggg cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg
     1261 cccatcaccg gctttttgca tatgccgaaa ggcgatggcc cgttcccgac agtattaatg
     1321 tgtggtggtc tggatgcgat gcagacggac tattacagcc tgtatgaacg ttattttgcg
     1381 ccgcgcggca ttgcgatgct gactattgat atgccgtcgg tgggcttttc ttcaaaatgg
     1441 aagctcaccc aggactccag cctgttgcat cagcacgtct taaaggcgct gcctaacgta
     1501 ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc
     1561 gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta
     1621 gttcataccc tgttgagtga ttttaagtgc cagcaacagg tgccggaaat gtatcttgac
     1681 gttctggcga gtcgtttggg gatgcatgat gcttccgacg aagcgttacg cgtggagctg
     1741 aatcgctatt cattaaaagt gcaaggattg ctggg
//
LOCUS       DAJTRN010000440         1405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.271, whole genome shotgun sequence.
ACCESSION   DAJTRN010000440 DAJTRN010000000
VERSION     DAJTRN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.271"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..496
                     /gene="sat2"
                     /locus_tag="OO328_004405"
     CDS             <1..496
                     /gene="sat2"
                     /locus_tag="OO328_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5932693.1"
                     /translation="AETLDAENHFIVREVFDVHLSDQGFELSTRSVSPYRKDYISDDD
                     SDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSLIEFAKKWA
                     LSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGA
                     QDDA"
     gene            554..1345
                     /gene="aadA1"
                     /locus_tag="OO328_004406"
     CDS             554..1345
                     /gene="aadA1"
                     /locus_tag="OO328_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5932694.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          363 a          349 c          372 g          321 t
ORIGIN      
        1 ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct
       61 atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat
      121 ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct
      181 tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt
      241 tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa
      301 gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc
      361 tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta
      421 taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc
      481 acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca
      541 ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      601 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      661 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
      721 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      781 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      841 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      901 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      961 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     1021 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     1081 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1141 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1201 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1261 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga
     1321 gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc
     1381 ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAJTRN010000441         1402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600617
            SAMEA8900386-rid15818663.guided.273, whole genome shotgun sequence.
ACCESSION   DAJTRN010000441 DAJTRN010000000
VERSION     DAJTRN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900386
            Sequence Read Archive: ERR6004791
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477705.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:03:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,408
            CDSs (total)                      :: 4,341
            Genes (coding)                    :: 3,980
            CDSs (with protein)               :: 3,980
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 170 of 361
            Pseudo Genes (incomplete)         :: 87 of 361
            Pseudo Genes (internal stop)      :: 141 of 361
            Pseudo Genes (multiple problems)  :: 36 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900386-rid15818663.guided.273"
                     /serovar="S. flexneri 2a"
                     /isolate="600617"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-02"
     gene            <1..496
                     /gene="sat2"
                     /locus_tag="OO328_004407"
     CDS             <1..496
                     /gene="sat2"
                     /locus_tag="OO328_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5932695.1"
                     /translation="AETLDAENHFIVREVFDVHLSDQGFELSTRSVSPYRKDYISDDD
                     SDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSLIEFAKKWA
                     LSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGA
                     QDDA"
     gene            554..1342
                     /gene="aadA1"
                     /locus_tag="OO328_004408"
     CDS             554..1342
                     /gene="aadA1"
                     /locus_tag="OO328_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5932696.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          360 a          349 c          372 g          321 t
ORIGIN      
        1 ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct
       61 atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat
      121 ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct
      181 tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt
      241 tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa
      301 gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc
      361 tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta
      421 taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc
      481 acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca
      541 ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      601 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      661 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
      721 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      781 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      841 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      901 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      961 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     1021 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     1081 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1141 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1201 gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg gacaagaaga
     1261 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga aaggcgagat
     1321 caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc gcttcgcggc
     1381 gcggcttaac tcaagcgtta ga
//
